LOCUS       DAYYNG010000001        61102 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000001 DAYYNG010000000
VERSION     DAYYNG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.001"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHBFA_000001"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHBFA_000001"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9393532.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACHBFA_000002"
     CDS             complement(937..1518)
                     /locus_tag="ACHBFA_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9393533.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHBFA_000003"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHBFA_000003"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9393534.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHBFA_000004"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHBFA_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9393535.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHBFA_000005"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHBFA_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9393536.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACHBFA_000006"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACHBFA_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9393537.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACHBFA_000007"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACHBFA_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9393538.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHBFA_000008"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHBFA_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9393539.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACHBFA_000009"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACHBFA_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9393540.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACHBFA_000010"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACHBFA_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9393541.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACHBFA_000011"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACHBFA_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9393542.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACHBFA_000012"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACHBFA_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9393543.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHBFA_000013"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHBFA_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9393544.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACHBFA_000014"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACHBFA_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9393545.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHBFA_000015"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHBFA_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9393546.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACHBFA_000016"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACHBFA_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9393547.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHBFA_000017"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHBFA_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9393548.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHBFA_000018"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHBFA_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9393549.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACHBFA_000019"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACHBFA_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9393550.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACHBFA_000020"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACHBFA_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9393551.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACHBFA_000021"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACHBFA_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9393552.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACHBFA_000022"
     CDS             13972..14157
                     /locus_tag="ACHBFA_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM9393553.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHBFA_000023"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHBFA_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9393554.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACHBFA_000024"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACHBFA_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9393555.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACHBFA_000025"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACHBFA_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9393556.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHBFA_000026"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHBFA_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9393557.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHBFA_000027"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHBFA_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9393558.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACHBFA_000028"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACHBFA_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9393559.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACHBFA_000029"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACHBFA_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9393560.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACHBFA_000030"
     CDS             18232..18879
                     /locus_tag="ACHBFA_000030"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9393561.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACHBFA_000031"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACHBFA_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9393562.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHBFA_000032"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHBFA_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9393563.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHBFA_000033"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHBFA_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9393564.1"
                     /translation="MARIAGVDIPRDKHVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHBFA_000034"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHBFA_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9393565.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACHBFA_000035"
     CDS             20317..21255
                     /locus_tag="ACHBFA_000035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9393566.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHBFA_000036"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHBFA_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9393567.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACHBFA_000037"
     CDS             21935..22774
                     /locus_tag="ACHBFA_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9393568.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACHBFA_000038"
     CDS             22750..23619
                     /locus_tag="ACHBFA_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9393569.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACHBFA_000039"
     CDS             23609..24406
                     /locus_tag="ACHBFA_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9393570.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACHBFA_000040"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACHBFA_000040"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9393571.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="ACHBFA_000041"
     CDS             complement(25688..28357)
                     /locus_tag="ACHBFA_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9393572.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /gene="rpmB"
                     /locus_tag="ACHBFA_000042"
     CDS             complement(28595..28783)
                     /gene="rpmB"
                     /locus_tag="ACHBFA_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9393573.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="ACHBFA_000043"
     CDS             29053..29415
                     /locus_tag="ACHBFA_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9393574.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="ACHBFA_000044"
     CDS             29467..31149
                     /locus_tag="ACHBFA_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9393575.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="ACHBFA_000045"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="ACHBFA_000045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9393576.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="ACHBFA_000046"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="ACHBFA_000046"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9393577.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="ACHBFA_000047"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="ACHBFA_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9393578.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="ACHBFA_000048"
     CDS             34984..35988
                     /locus_tag="ACHBFA_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393579.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="ACHBFA_000049"
     CDS             35989..36933
                     /locus_tag="ACHBFA_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393580.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /gene="opp4B"
                     /locus_tag="ACHBFA_000050"
     CDS             36933..37895
                     /gene="opp4B"
                     /locus_tag="ACHBFA_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9393581.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="ACHBFA_000051"
     CDS             37922..38836
                     /locus_tag="ACHBFA_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9393582.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="ACHBFA_000052"
     CDS             38862..40643
                     /locus_tag="ACHBFA_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9393583.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /gene="rnc"
                     /locus_tag="ACHBFA_000053"
     CDS             40991..41677
                     /gene="rnc"
                     /locus_tag="ACHBFA_000053"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9393584.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="ACHBFA_000054"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="ACHBFA_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9393585.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="ACHBFA_000055"
     CDS             45287..46096
                     /locus_tag="ACHBFA_000055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9393586.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="ACHBFA_000056"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="ACHBFA_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9393587.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="ACHBFA_000057"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="ACHBFA_000057"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM9393588.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="ACHBFA_000058"
     CDS             complement(49648..50100)
                     /locus_tag="ACHBFA_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9393589.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="ACHBFA_000059"
     CDS             50250..51200
                     /locus_tag="ACHBFA_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9393590.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="ACHBFA_000060"
     CDS             51193..52152
                     /locus_tag="ACHBFA_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9393591.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="ACHBFA_000061"
     CDS             52149..52904
                     /locus_tag="ACHBFA_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393592.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="ACHBFA_000062"
     CDS             52927..53880
                     /locus_tag="ACHBFA_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9393593.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54524..54820)
                     /locus_tag="ACHBFA_000063"
     CDS             complement(54524..54820)
                     /locus_tag="ACHBFA_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM9393594.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55175..55531
                     /locus_tag="ACHBFA_000064"
     CDS             55175..55531
                     /locus_tag="ACHBFA_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9393595.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55541..56440
                     /locus_tag="ACHBFA_000065"
     CDS             55541..56440
                     /locus_tag="ACHBFA_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9393596.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            56960..57688
                     /locus_tag="ACHBFA_000066"
     CDS             56960..57688
                     /locus_tag="ACHBFA_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9393597.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            57833..59860
                     /locus_tag="ACHBFA_000067"
     CDS             57833..59860
                     /locus_tag="ACHBFA_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9393598.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            59968..61065
                     /locus_tag="ACHBFA_000068"
     CDS             59968..61065
                     /locus_tag="ACHBFA_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9393599.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT        20145 a        10969 c        13053 g        16935 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacatgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    54301 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacgatgtt ccatctgctg
    54361 cttctcggca ataagcccac tagactagaa aatatgagaa gctattttct gtccagcgtt
    54421 cttattactc aaagcaaatc gcagttgtca caatctcttc taaacaatgt tcaatcagct
    54481 gaccttcggt attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag
    54541 caagcggttg atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc
    54601 accgttttaa taattgatag aaataactct cttctgcttt gattttatac tcgcttaata
    54661 tcaatccaaa actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg
    54721 ttttatcttc attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg
    54781 tttcttcgat aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt
    54841 cttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa caaaaagtaa
    54901 ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt gttatttaag
    54961 aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg ttcaagaaac
    55021 tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa taaacgaatg
    55081 acttactttc caataaattg agaacagtca gaaccagaca aattagttgc atctgatgaa
    55141 taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga caaaagaagc
    55201 acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag atatcgaaaa
    55261 cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggatacgg catttcgatt
    55321 gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag tggctgatac
    55381 accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg atccatccac
    55441 acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg gcttgagaaa
    55501 aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat tggcgatcat
    55561 cagcgaccta catgtggaca tcaataaatt cggcgaatca gagctgatgc agctttggca
    55621 agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg ctaataaagt
    55681 agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta cgacatttca
    55741 tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc attttccaga
    55801 cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc ttttagggat
    55861 gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa tcgtacgaat
    55921 gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa
    55981 tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa aaaaacttga
    56041 ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc aatctgctcc
    56101 ttatgagcgt tggaacaaat taaatgcatt tttaggatca gaatcattcg gagaattact
    56161 tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc gctttgaaga
    56221 taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt atgaatggca
    56281 gctgacccga gattttgtct tgaaagaaca gttgctagac agctatgatc ctatgaaatt
    56341 acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac aacatttgca
    56401 agaggaattt gaacaagcga tgacaatcgt tccgtattga gacagcatta gtaatgtgta
    56461 aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat ttatcgtgga
    56521 tatgctgctt tttttgcgga aaataatcag cgaatgttta tttgatcgga tgatttgtaa
    56581 ctcgcagagt actagatgca agcgtgtatt ctttttttga acgataatca caaaaaaaaa
    56641 tataatatac attttttatt taattataat taatattgat tgaagtctag gcagtcaagt
    56701 cacattttga tagataggag ggggacttac gaattatttt aaaggctatg acagctgtat
    56761 ttctagattg tgtttgcatc aagttgcaat aaaaactaga aatcaaatcg ggctgataat
    56821 catttataac taatcgatac ataggaccaa atataggtct atgttctttt atttcataag
    56881 tatcgtaaaa cagaattttg ctatggatta atccaatcga ctgatatgtt tagtaaataa
    56941 agaaagagga atgtcaatga tgaaaaaaga aatcgtatta acagcagcaa tgatgttatt
    57001 cagcatggta gcaagtacga cttttgtttc tgcaactgaa gtatacccaa aggaatataa
    57061 cacagaagga acgattacat ttgaagcagg agatgagggt gtaactcctc ctgttgatcc
    57121 agagaatcct gatcccaata aacctgtcga tccatcagat cctccaagcc ctggaacagg
    57181 aggggcatta tcgattgatt atggatcaaa attcaaattt gggactcaaa aaatttctac
    57241 tgcggataaa acgtattatg cagctgctga tgtgatgaac gatgggtcta gaaaaccgac
    57301 atatgtccaa gtgacagata ggagaagtac cttatctggg tggaaattaa gtgtctctca
    57361 accagaacag tttaaaactg ccagtgggga tgaactagtt ggagcacagc tgaaatttac
    57421 gaaagggcag gctgtttctt tggttgatcc aacatatacg ccgcaaacgg tgaattctga
    57481 attaacgttg acaccgggag gaaacaatac gttagcaatt aatgctaaat caggaacagg
    57541 tgtggggact tgggtctatc gatttggtgc aaatgaaaat gaaaatcaag atgcggtaca
    57601 gctttcggta cccggtaaat ctgtcaagtt agcgcaacaa tattccacta aattagtttg
    57661 gacattagaa gatacaccta acaactagtt tactgaaaag aactgttaaa aataaatacg
    57721 tagtcatatt gtcttgcgag gtaatcgagc aaaaattgtc aaaggctagg ctaaatttgt
    57781 ttggatacga atagttcatc taggaattta acgatcagga ggaaaagata tattgaaaaa
    57841 gaggaggatc gtgctcattt taagcagtct cttagtgact aatatctttc cgatcatagg
    57901 gtactctttg gaattgaatc agttggagca tggaaataga agagacgaag aaagcaaact
    57961 cacttcgaca ttcgacagtt taatagaatc caaccagaca caagaaagca gtgaggattt
    58021 aagtatttcg agtagtggag agaccactga caacgtagaa gctgagggtt cggacaaaca
    58081 aggactagaa gatgcaccaa tttctgcggt tgagctgcct gacgatccca atattatttc
    58141 tatcgataaa atttttcaaa cacctatcgg tactagcacg tccatattgg aaaatgggaa
    58201 gttactgcag ttgaacccag cagaaaggtc tcaaagagga gctatttggt caaataagaa
    58261 gattagttta ctctcggatt ttacatttaa atcttattta tacttaggaa atcaacgaga
    58321 tagagcggga gatggcatga cctttacatt gactggcgat cctagaatgg ctagtaatcc
    58381 cagtcaagtt attggatcgc caggtatggg aattggctca tattctactc gttctggcca
    58441 accttacatt gcgaatgcgc tctcgattga gtttgatacg tataaaaata gtggttcttc
    58501 tgatcgaatg gatcgagaga taactgctga taatagaggg catggacatg tcgcttttgt
    58561 cacgcctaaa ccaaataata ataattattc tggagagcat actggcgtga cggtagctcc
    58621 tacatttctt tctaatggca catggcgctt gttaataatt aaatgggacg cggagcaaag
    58681 aagtcttact tatgatttgg aaggtgtcgg tcactcttct cggattatac ctgatctcat
    58741 taggcagttt ggttctacag aagtaaattg ggggtttact tcttcaacag gtactttttt
    58801 tcaagaaaat gctgtcgctg tcacgcgact tccatcgaat gtcacatcaa cagctgatgt
    58861 aaaagtcaac gatagtgatt atggggccat cgctgaagct ggaggaaacg ataagataac
    58921 tatcagaaat acattgaagg tcgaggggga ttttcttgaa tataagaaaa tgcagactag
    58981 tatcgacctc ccacaagaat taactattcc cgagcatatc ttgatcaatg gaaatcaaat
    59041 tgatgccaaa gatatcttga taaccggaaa tcgagtcacg ctaaatctga cagattattt
    59101 tattgctgga gatacgttaa taatcgaatt ggagacaact ttgcaggaag taattcctga
    59161 aaaagtactc actatgcatt ttgaatacct tgaaaacggg aatttgattg agcaatcgaa
    59221 tgaaataaac ctaaagatcg caaagctgaa ggagatagat atccatgttt attataaaga
    59281 taaagacagt cagattgata ttgctgaaat gaaaacaatc actgggaaaa taggagagaa
    59341 gtataaagaa actcctattg atattgaagg gtatgttttt gactcggatt cagcgaatac
    59401 agaaggcatc ttctccgaaa atacagagga tatctatttt tattacagaa aaggcaaact
    59461 ttatctctca actgctcctc aatatgtcaa ttttggaaaa cataaaatat ccagtagacc
    59521 attaagtgaa ttcggacagt tttcaggtgg gttgaaaatt atagatgaac gggcttttgg
    59581 aagttggaaa ttacaattga aacaagagaa agttcttaca aatggagatt ttgagcttcc
    59641 agaagctctt tcctttgtta cctctgaaaa tacaacggtt atagggacat ccgctgttac
    59701 gattttcgaa agtgatcaaa agggtgaaag cgatttgtcc tctcttttag attctacagc
    59761 tcatcgtgga atcagaatag atattcctgt agaaaaccaa agaatcggaa cctttgaggg
    59821 gaaactatct tggatacttg cagatgtccc aggaaattaa caggaagaga agaatataac
    59881 aaaattattg agtttcaaga agggcacagg taccataggc tcttgtgtcc cacagagaca
    59941 agattttgtt atagtcttct cttacatatg attaaaggag cgattttctt gagaccaaaa
    60001 caacaattat ttataacact cctagttatt ttaggggcac tttttaacaa tatatcagtg
    60061 tcagctagtg catttaattt ttcagtgacg ccgataactt cagagaatca aatcgacaag
    60121 agaaaaactt acttcgatct tcaacttgat ccggatcagg aagtagaagt aaaagcggaa
    60181 ttgagaaatg atacagaaaa agaagtgaaa atcgatattt ctgtgaatag cgcaaccacc
    60241 aattcaaatg tgatggttga atacggaaaa aacgagattg aaaaagacat gagcctgatc
    60301 tttgatctgg tagattatgt gagctatcca cagatagtga cgctaaaacc taaaagtgtc
    60361 caaaccgttg cttttcatgt caggatgccg aatgagcgat ttgatggtgt attggctggt
    60421 gggatcacat tccaagaaat ccagacggaa aaggaccaaa cagaaactaa ggatcaaagc
    60481 ttatcgattg agaatgaata cacctatatt attgctttgt tgatgcaaca gacactgaac
    60541 gaagttgctc cgaaccttac tcttcatgaa gttaaaccag atcaaatcaa tgcaagaaat
    60601 gtcatcttag caaacgttca aaatgatcaa aaaacctata tcaatcaagt agttattgaa
    60661 acaaaaatta caaaaaaagg acattctgaa gttttatatc aagaggaaaa agaaggattg
    60721 cagattgcgc cgaatacaaa tttttctttt ccaactgctt taaatggtca accgctaacc
    60781 ccaggtgaat atcaccttac aatgactgtt ttaggaaatg aaaatgaaaa tggaaaattt
    60841 tcgagaaaaa aaggaaatac tacgatcaac tataccaatc aatgggtatt tgaaaaagat
    60901 tttacaatag acggaaaagt agccaaggaa ttgaacacga cggatgttac gattaaaaaa
    60961 gacaataatt ggatttatat cttagtagct tcacttttct tattgtttat tttattatgc
    61021 atacttttgt ttgttttgag aaaaaaagaa gagaaagaaa agtaaggtta agataacaaa
    61081 tagtaaattt ttattattgt ag
//
LOCUS       DAYYNG010000002        47363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000002 DAYYNG010000000
VERSION     DAYYNG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.002"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..1172
                     /locus_tag="ACHBFA_000069"
     CDS             108..1172
                     /locus_tag="ACHBFA_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9393600.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1418..1843
                     /locus_tag="ACHBFA_000070"
     CDS             1418..1843
                     /locus_tag="ACHBFA_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9393601.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1836..2285
                     /gene="nusB"
                     /locus_tag="ACHBFA_000071"
     CDS             1836..2285
                     /gene="nusB"
                     /locus_tag="ACHBFA_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9393602.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2382..3236
                     /locus_tag="ACHBFA_000072"
     CDS             2382..3236
                     /locus_tag="ACHBFA_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9393603.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3237..4574
                     /gene="xseA"
                     /locus_tag="ACHBFA_000073"
     CDS             3237..4574
                     /gene="xseA"
                     /locus_tag="ACHBFA_000073"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9393604.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4579..4803
                     /locus_tag="ACHBFA_000074"
     CDS             4579..4803
                     /locus_tag="ACHBFA_000074"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9393605.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4805..5695
                     /locus_tag="ACHBFA_000075"
     CDS             4805..5695
                     /locus_tag="ACHBFA_000075"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9393606.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5704..6522
                     /locus_tag="ACHBFA_000076"
     CDS             5704..6522
                     /locus_tag="ACHBFA_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9393607.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6629..7078
                     /locus_tag="ACHBFA_000077"
     CDS             6629..7078
                     /locus_tag="ACHBFA_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9393608.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7096..8778
                     /gene="recN"
                     /locus_tag="ACHBFA_000078"
     CDS             7096..8778
                     /gene="recN"
                     /locus_tag="ACHBFA_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9393609.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8833..8949)
                     /locus_tag="ACHBFA_000079"
     CDS             complement(8833..8949)
                     /locus_tag="ACHBFA_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9393610.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9022..9978)
                     /locus_tag="ACHBFA_000080"
     CDS             complement(9022..9978)
                     /locus_tag="ACHBFA_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9393611.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10149..10517
                     /locus_tag="ACHBFA_000081"
     CDS             10149..10517
                     /locus_tag="ACHBFA_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9393612.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10687..11118
                     /gene="mraZ"
                     /locus_tag="ACHBFA_000082"
     CDS             10687..11118
                     /gene="mraZ"
                     /locus_tag="ACHBFA_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM9393613.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11139..12098
                     /gene="rsmH"
                     /locus_tag="ACHBFA_000083"
     CDS             11139..12098
                     /gene="rsmH"
                     /locus_tag="ACHBFA_000083"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9393614.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12103..12504
                     /gene="ftsL"
                     /locus_tag="ACHBFA_000084"
     CDS             12103..12504
                     /gene="ftsL"
                     /locus_tag="ACHBFA_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9393615.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12505..14697
                     /locus_tag="ACHBFA_000085"
     CDS             12505..14697
                     /locus_tag="ACHBFA_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9393616.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14723..15685
                     /gene="mraY"
                     /locus_tag="ACHBFA_000086"
     CDS             14723..15685
                     /gene="mraY"
                     /locus_tag="ACHBFA_000086"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9393617.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15699..17060
                     /gene="murD"
                     /locus_tag="ACHBFA_000087"
     CDS             15699..17060
                     /gene="murD"
                     /locus_tag="ACHBFA_000087"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9393618.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17086..18174
                     /gene="murG"
                     /locus_tag="ACHBFA_000088"
     CDS             17086..18174
                     /gene="murG"
                     /locus_tag="ACHBFA_000088"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9393619.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGAIKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18189..19409
                     /locus_tag="ACHBFA_000089"
     CDS             18189..19409
                     /locus_tag="ACHBFA_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM9393620.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19565..20890
                     /gene="ftsA"
                     /locus_tag="ACHBFA_000090"
     CDS             19565..20890
                     /gene="ftsA"
                     /locus_tag="ACHBFA_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9393621.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20913..22154
                     /gene="ftsZ"
                     /locus_tag="ACHBFA_000091"
     CDS             20913..22154
                     /gene="ftsZ"
                     /locus_tag="ACHBFA_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9393622.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22171..22848
                     /locus_tag="ACHBFA_000092"
     CDS             22171..22848
                     /locus_tag="ACHBFA_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9393623.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22862..23461
                     /locus_tag="ACHBFA_000093"
     CDS             22862..23461
                     /locus_tag="ACHBFA_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9393624.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23542..23763
                     /locus_tag="ACHBFA_000094"
     CDS             23542..23763
                     /locus_tag="ACHBFA_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9393625.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23812..24594
                     /locus_tag="ACHBFA_000095"
     CDS             23812..24594
                     /locus_tag="ACHBFA_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9393626.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24615..25322
                     /locus_tag="ACHBFA_000096"
     CDS             24615..25322
                     /locus_tag="ACHBFA_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM9393627.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25584..28382
                     /gene="ileS"
                     /locus_tag="ACHBFA_000097"
     CDS             25584..28382
                     /gene="ileS"
                     /locus_tag="ACHBFA_000097"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9393628.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28431..29957)
                     /gene="zwf"
                     /locus_tag="ACHBFA_000098"
     CDS             complement(28431..29957)
                     /gene="zwf"
                     /locus_tag="ACHBFA_000098"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9393629.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29972..30619)
                     /locus_tag="ACHBFA_000099"
     CDS             complement(29972..30619)
                     /locus_tag="ACHBFA_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9393630.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30803..31132)
                     /locus_tag="ACHBFA_000100"
     CDS             complement(30803..31132)
                     /locus_tag="ACHBFA_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM9393631.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31309..32037
                     /gene="sfsA"
                     /locus_tag="ACHBFA_000101"
     CDS             31309..32037
                     /gene="sfsA"
                     /locus_tag="ACHBFA_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM9393632.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32053..33066
                     /locus_tag="ACHBFA_000102"
     CDS             32053..33066
                     /locus_tag="ACHBFA_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9393633.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33066..34343
                     /locus_tag="ACHBFA_000103"
     CDS             33066..34343
                     /locus_tag="ACHBFA_000103"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9393634.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34406..37108
                     /locus_tag="ACHBFA_000104"
     CDS             34406..37108
                     /locus_tag="ACHBFA_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9393635.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37260..37577
                     /locus_tag="ACHBFA_000105"
     CDS             37260..37577
                     /locus_tag="ACHBFA_000105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9393636.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37607..37927
                     /locus_tag="ACHBFA_000106"
     CDS             37607..37927
                     /locus_tag="ACHBFA_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9393637.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38035..39495
                     /locus_tag="ACHBFA_000107"
     CDS             38035..39495
                     /locus_tag="ACHBFA_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9393638.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39563..39784
                     /locus_tag="ACHBFA_000108"
     CDS             39563..39784
                     /locus_tag="ACHBFA_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393639.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39815..39997
                     /locus_tag="ACHBFA_000109"
     CDS             39815..39997
                     /locus_tag="ACHBFA_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM9393640.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39997..40410
                     /locus_tag="ACHBFA_000110"
     CDS             39997..40410
                     /locus_tag="ACHBFA_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM9393641.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40533..41714
                     /locus_tag="ACHBFA_000111"
     CDS             40533..41714
                     /locus_tag="ACHBFA_000111"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9393642.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41785..41949)
                     /locus_tag="ACHBFA_000112"
     CDS             complement(41785..41949)
                     /locus_tag="ACHBFA_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393643.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42103..42176
                     /locus_tag="ACHBFA_000113"
     tRNA            42103..42176
                     /locus_tag="ACHBFA_000113"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42137..42139,aa:Arg,seq:cct)
     gene            complement(42222..42335)
                     /locus_tag="ACHBFA_000114"
                     /pseudo
     CDS             complement(42222..42335)
                     /locus_tag="ACHBFA_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(42718..43950)
                     /locus_tag="ACHBFA_000115"
     CDS             complement(42718..43950)
                     /locus_tag="ACHBFA_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9393644.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            44204..44773
                     /locus_tag="ACHBFA_000116"
     CDS             44204..44773
                     /locus_tag="ACHBFA_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9393645.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(44951..45391)
                     /locus_tag="ACHBFA_000117"
     CDS             complement(44951..45391)
                     /locus_tag="ACHBFA_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9393646.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            45549..46313
                     /gene="map"
                     /locus_tag="ACHBFA_000118"
     CDS             45549..46313
                     /gene="map"
                     /locus_tag="ACHBFA_000118"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM9393647.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            46345..47268
                     /locus_tag="ACHBFA_000119"
     CDS             46345..47268
                     /locus_tag="ACHBFA_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9393648.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT        15690 a         8104 c        10090 g        13479 t
ORIGIN      
        1 tatttaaaat aaaaagaagt tttccggaaa tttttattta actgtcgtgt acgaagaaaa
       61 gtttgctata atggaaaaaa gcgtgcaaag aaagagggag tttcattatg atgctacgtg
      121 tagaaaaatt gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg gttaccagtc
      181 cgtacaatct tcgttactta acgaacttta caggtacgac aggattagct gttattactt
      241 tggaaaaagc attttttatt actgattttc gttataccga gcaggcggca gcgcaggctc
      301 aagggtttga aattattaaa aatgtcggac ctatttttga tgaggtagca aacttagtac
      361 gcaaagaaga actgcactca ttagcttttg aggaaacaac agtctcattt ttagaatatt
      421 cggttttaga agaaatcatc gatgcggaat tagtacctgt ttcacagatg atcgaagaac
      481 tcagagaagt gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt catattgcgg
      541 atatggcata tgatcacatc ctgaaaatga tccaaccggg catgactgag attgaagtcg
      601 caaatcaact agacttttat atgcgttctc ttggcgcttc aggtgtttct tttgatacga
      661 tcgttgctag cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg
      721 aacaaggtga cttgatcaca ttggactttg gttgctatta cgaagggtac gtttccgata
      781 tgacacgtac attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag
      841 tcttagatgc tcaattagca gttattgatg ctgcaaaacc aggaatgact ggtgtccaat
      901 tagatgcagt tgctagagat tatatttcaa aacatggtta cggggaagca tttggacatt
      961 ctacaggaca tgggatcggt ttagagatcc acgaaggacc aaacgtttct tctcgtgcag
     1021 aaaagcaatt tgtaccagga aatgtgatca ctgatgaacc agggatctac ttaccaggaa
     1081 tcggtggagt acgtattgag gatgacttgt tgatcacaga agatggaaat cgtgtgttga
     1141 ctcgttcacc aaaagaattg atcatcttat agtttcatca atagaagaag taagcgtaaa
     1201 aatcagtgtt cacagacttt gttatagaaa atcactcgat ttaatgatac actatagaag
     1261 aacttttaag ctgtaagaga taataaagtt gggtgttttc tccaattcat agcagtttgc
     1321 agctgatcgg cggcagtttt cttaccacgt gatcactatg ccgcatcata gagaattcgg
     1381 atgtatctcg tgcttaggca aagggaggaa taaataaatg gcagatgaaa aaaatttgat
     1441 tttaaacgca aaccaagatt taggagagat cgtgatcgct cctgaagtca tcgaagtaat
     1501 tatcgggatt gcagcttcta aagtagaagg cgtatacgga atgcgcggaa cttttgccag
     1561 caatgtcact gaattattag gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga
     1621 ggaagatggc ttgaaagtag atctttactg ttatctagaa tatggtgttt cagttcctaa
     1681 agtagcaatg gaaatgcagg aacgggtcag ccagcaagtc cttttcatga cggatatcga
     1741 tttatctgaa gtcaatatcc atgtagtagc tgttgttcct gaaaaattag aacaaccaac
     1801 aatcgaagaa ctttttgaag acgaagagga agagaatgaa taaagaatta tcaagacacg
     1861 agattcggga aatggcattg caggctttat ttccattaga cttcaatgca gacctaacaa
     1921 aagaagatgc gattttcaac gcaatcgaat tagaccaccg cgacatgatc aacgaagatg
     1981 aatcagagtt tgttcctgtt tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag
     2041 atgaattgga taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc aatcggatct
     2101 caaaaatgga tttggtgatc ttgcgtatcg cgatttttga aatgacttat gtgacagatg
     2161 taccagcggc tgttgcttta aatgaagcaa tcgagttggc aaaaacattt agtgatgacc
     2221 gttcacgtaa attcgtcaac ggtgttttat ccaatgtatt gaaagaatta gaagctgggg
     2281 cgtaagtcct agcttttttc tatgaatcat cataaacctg ttataaatcg ggaaaattct
     2341 atggtagaat gaaaacacaa aaaatatctg ggagtggtta gatggcagaa ttgattaacg
     2401 gaaaagaatt agctgaaaaa atgcaggcag agattgctga aaaaatccaa aaattgaaag
     2461 ataatggtat ccatcccgga ttagtcgttt tattggtagg ggaaaatcca gccagccaaa
     2521 tctatgtaag aaataaagaa cgtgtagcaa aagaaatcgg aatccattca ttggtagagc
     2581 gttatcctga gacgatttct gaagacgaac tacttgcaga aattgaaaaa tacaatcaag
     2641 atcctagttt tcatggtata ttggtccaat taccattgcc aaagcatata gatgaagaaa
     2701 agatcctgtt agctatccgc cctgaaaaag acgtagacgg ttttcatccg atgaatttag
     2761 gcagactcct tgcgggtaaa ccggacaaga ttccttgtac tccatacggc atcatgaaaa
     2821 tgtttgaagc ttattctatc gatccggcgg gtaaacgagc agtagttatc ggacgaagca
     2881 atatcgtcgg taaaccgatg gctcagctac tcttgatggc agatgcaact gttactgttg
     2941 ctcattctaa aaccgaaaat ctggctgaac tggcacaaga agcagatatt ttagtcgtag
     3001 caataggacg tggacatttt gtcacaaaag aattcgtaaa accaggtgct gtagtgatag
     3061 atgtagggat gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa tttgatgaag
     3121 tggagcctat tgcaggctgg atcactcctg tgccaaaggg tgtagggcca atgactatca
     3181 cgatgctgat gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg
     3241 cacaagaata tttaactgtc actacattaa cgaagtattt gaaaagaaaa tttgatgccg
     3301 atccttattt agaacgcgta tatttaacag gcgaaatatc gaatttccgt ttacgtgcca
     3361 atgcccatca atatttcagc ttaaaagatg accatgcaaa aatatctgct attatgttca
     3421 aaggagcatt ccaaaaatta cgattccagc caaaagaagg aatgaaagtc cttgtcgttg
     3481 ggcgtatttc attatatgaa gcgggaggtt cttatcaaat ctatgtagag cacatggagc
     3541 ctgatggaat aggtgcttta tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg
     3601 aaggcctttt tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc
     3661 ttaccagtcc aagcggagcg gttatccgcg atatcatcac gacggtgaaa agaagatatc
     3721 caattgtcca attggttttg ttcccaacag ttgtacaagg aaaccaagca gcggacaatg
     3781 ttgtcaggaa tatccagaga gtagaagaga tgggcaattt cgatacaatg atcatcggtc
     3841 gtgggggtgg ttcgattgag gatttatggc cgtttaatga agagcgggtt gctcgggcga
     3901 tttatcaagc taatacgccg gtgatttcat ctgtaggaca tgaaacagat accacgatcg
     3961 ctgatttagt agcagatgtt cgggctgcca ctccaacagc agctgcagaa ttagctgttc
     4021 ctgttttgtc agaagagttg atgcgcattc aagaacggca agctagattg cagcaggcgt
     4081 ttgtacgcca gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat
     4141 ttcggcagcc tgaaagattg tatgaaggtc agaccatcaa attagaccag ttgaaacagc
     4201 gattatatca agctacacag caaatttatc atgcgaaaga aaaacaaacg atcgcattaa
     4261 gccaccaatt ggaacaagtg gctccgcttt atcgagttaa agcagctcgc caagaaactg
     4321 cttatctaga aaaacgctta gaagagaaaa tccaacaata tatgaaacaa cagcagcagc
     4381 gttttgctca agctgttcaa tctcttgatt tgttgagtcc attgaaaatc atgggaagag
     4441 gctacagcta tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag attcatcaag
     4501 gggatttgct gacgatccat tatcaagatg gaatcgttaa aggacaagta caagagatag
     4561 acaaaggaga ataaaaatat ggaaaatcca acttttgaag agtcactgca agaattagaa
     4621 aaaattgtca tgcagttaga acaaggagac gttccattag aatcagctct agattcgttt
     4681 aaacgaggaa tggaattaag caaacattgc caagatacat tagctaaagc agaagagacc
     4741 ttgacgaaaa tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc
     4801 tgagatgaga ttaactgact tttcagctat tcatctacct gctgtagaaa aagaaatctt
     4861 gtctttttta gatgagcata caacggatag aggactctat gatgcgatga gttattcagt
     4921 gaaagcaggc gggaaaagaa tacgtccctt attactttta acggccgttg cttcctttga
     4981 tgagcctatt gatgttcccg tctatcaagt agcagctgct ttagaaatgg tgcatacgta
     5041 ttccttgatc cacgatgatt tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc
     5101 taccaatcat aaagtattcg gcgaagcatt agctatattg gccggagatg gtttgcttac
     5161 aggagcattc cagctaatca gtatggcaca cttaggtaat tctcctaagc ttctgctgct
     5221 tcagcaatta gctgtttgtg cgggatcaca aggtatggtt gctgggcaag ctgcagatat
     5281 cgagggcgaa tcgaaaaagc tttctttaga agaattagca tttattcatg aaagaaagac
     5341 agggcatttg attcgttatg ccttgctggc tggtggcata ttagcgaagc aacctgaaga
     5401 aatcttattg ctacttcaac gattagccga acatttaggt ttagcttttc agataagaga
     5461 tgatctactc gatgtgattg gtacaacgaa aacattaggg aaaacagctg gtaaagatga
     5521 acgtatggaa aaaaatactt acccgcgttt attaggccta gaaaagacac gtgaggcgtt
     5581 ggagattgag ctgttatccg ccaacaagat catagacaaa ttagaagagg acgttttttc
     5641 ttttgatggc gggctgatgc gccagatgat caagcaattt gatgtggaga aatgagggaa
     5701 agaatggaaa aagaacgcgt agatgtatta gcggtcaaac aaggattatt tgaaacaaga
     5761 gaacaagcca aacgttcagt gatggctggt ttgatttaca acgaaaaaaa cgagcggttt
     5821 gataaaccgg gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg taaaaaactt
     5881 ccttatgttt ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt tgatctatct
     5941 gttgaaggaa aaacattgtt agatattggt gcatccactg gcggatttac tgatgcagct
     6001 ttgcaaaatg gcgcaaaaat gagttatgct ttagacgttg gctacaatca gctagcttgg
     6061 aaaataagac aagatcctag agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa
     6121 cccgaagact ttaccttagg aattccagaa gttgctgtta tcgatgtttc ttttatttca
     6181 ctaaaactga tgctgcctcc attacatgct attttaaaag aagagggaga agtgattgct
     6241 ttaatcaaac cccaattcga agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt
     6301 gaccctcaga cccatcaaaa agttttagaa gacattttgt catttgcggc acaaggtgga
     6361 tatgatgtgc tggaattaag ttattctccg atcacggggg gagaaggaaa tatcgagttc
     6421 ttggctcact tgaggaaagt acccgaaagc ggaacaatca attcagctat taatatggca
     6481 gaagtggttt caaatgcaca cgaacagttt gaccataaat aaattgtaac tgtgaaaatc
     6541 aagtgagaag tatgcggatt cttctttatt ataccaagga aataagctat cattgattaa
     6601 gaagaatatg ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc gtttgattac
     6661 tcgattgttg agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag
     6721 caaaggaata tctgttactc aagctacgat ttccagagat ataaaggaac tgaaactgat
     6781 caaggtaccg gcttcagatg gcggctaccg ttatagcata ccagctgaaa caaacgaaga
     6841 tgtaaatact aaattggaaa aactattaaa agacgctttc gtggcagttg accaaatgga
     6901 aaaatttgtt attttgaaaa cattgccggg aaatgcttcg gcagcagcaa atttgattga
     6961 taaacgttac aagaaagaat tattttcgat tatcaatgat gacgataatg ttttgatgat
     7021 tacacgaact gaagaccagg cagttttatt gaagaaagac ttcctgcatt acctttaagt
     7081 tgaggagcga atgtgatgct acaagaaatc agtattacta actttgcgat tattccggaa
     7141 ttgcggcttt cttttcacga aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa
     7201 tccattatta tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag cgattatata
     7261 cgtcaaggag cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa acaagaagga
     7321 ttctctgaac tgatggttga attagggatc gaaacagatg aggacaatct gattgttcga
     7381 cgggatatgt ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat tatcacatta
     7441 gctaatctga gaagaatcgg cagttattta gtagatatcc aaggacaaaa cgaacatcag
     7501 gaactgctcc agcctgaatc acatcttgct ttattggatc gttttggaga tgtagctttc
     7561 caacagaaga aaaagagtta tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg
     7621 gtacgaaaag tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat gctccatttc
     7681 cagcaagaag aaatagctgc tgctgatttg caagtcggag aagaagagaa attaaaagaa
     7741 gaaagagaaa aacttagcaa ttatcaaaaa attgctgatg gattagctgc aggttacgga
     7801 gcactgacga ataatgaaca aaacagtgta gatggtgttg gcttggccgt ttcagaactg
     7861 caagggatcg cccatctaga tgtggagtat gaagcaattt atgaaaatat ccaaagtgct
     7921 tactatttgc tgcaagatgc aatcggcgat atgagtcgtc agattgattt attggagtta
     7981 gatgagagtc gtttagaaga agttacgcaa cgtttagaat taatccgaca gttgaaacgt
     8041 aaatacggcg aatccattga gtcaatattg gcttactatg acgaaatcac cgaagagcta
     8101 gcttcttctg atttttcaga aggtcaattg gacaaaatga aagaggaact agagcaaaaa
     8161 gagcttctac ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa
     8221 gaattggaaa aatccattct gcatgaattg aaaagtctgt atatggaaaa cactgagttt
     8281 gaggtacgtt tcttaaaaga agaaaataga caattgaatc atgatggatt tgaccaaatc
     8341 gaattttaca ttacgaccaa tccaggagag ccactgaagc ctttggtgaa agtcgcttct
     8401 ggtggcgaac tgtctcggat gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt
     8461 gtgacaagta ttatttttga tgaagtggat acaggtgtga gcggaagagt tgcacaggcc
     8521 atcgcagata aaatttctca aatatcgaaa tactctcaag ttttgtgcat cactcatctg
     8581 cctcaagttg ctgcagtcgc cgactatcaa tattatattg tgaaagcagt gattgatgga
     8641 cgtacgcaaa cttctgtttc agagttgaaa acaaaagaaa gagaagaaga aattgcacgt
     8701 atgctggctg gaagtgagat tacaaaactc acactcgaac atgcaaaaga actattgaaa
     8761 ctcgcaaaaa ggacctgagc cacctcgcag tggttcaggt ccttttcagc agctgatagc
     8821 tatattttat ttttacatga cactcattac agcagtcaat acaacagatc caatcgttaa
     8881 aatcagcatt gtccaaatga caaattttgt tactttagca aaagtacttg tttgtttttt
     8941 ttcacgcatt gttttcaacc tcgtcattaa ttagttgatt caagtgtaca atcttttttc
     9001 catttccaca agagggcttt tttataaaaa cttccctttt tttaaataat aggtagttac
     9061 gatacaaata aataatgtta atagaactag ccatataaag gcatcatccc tttcagcaat
     9121 aggtaactta atgttcattc catagatact gccgacgatt gtcggtatag ttaaaataat
     9181 cgttaaagaa gttaaaattt tcataatatt attcagatta ttcgatacga ttgctgaaaa
     9241 agtctgattc atttgttcta ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc
     9301 tgtttccacc aatacatcac gtaaatgatt tttcaaagca tgattttctt ggaaaagttt
     9361 attttgcgct agttctttca atgcatctaa attagcagaa gtcgcttctc tgaaataaac
     9421 gaggcttttt tggatatcca tgatttgata aagttgtttg ttttcagtgg atacttgaag
     9481 tttggcttcc aaagtatcgc attgagattg aagcgtagct aaataactgt tgtaagagag
     9541 tgtgacctgc caaatgaatt gaaagaagat attgagatta ggcgaaatat cactttttgg
     9601 aaaggtctgg ctaaagactt tctttaaaaa cagtggttca tgattcgtaa ttgtgataag
     9661 cttctttttg ggcgtcaaaa taatagagag cgggtatgta tccacttgaa aataaccgct
     9721 aggacttgtt ttagcaaaag gatattgtaa caataataac gctggctttg tcaactttga
     9781 ttgattgaat tcttctgtac gggaattttc tgcatcgtcc aatatggaag tgatgtaatc
     9841 tgacggaatt tcaaattgat gtgtcagctg atgaatctct tcttgattag gacgttctac
     9901 acaataccag atcgtttggt cagtttcctt tccggtatta cgcagtttct cattatctaa
     9961 tgtaaaatat ttgatcatga ttttcctcct gttgcctgtt tcttttatta taacggatag
    10021 ggaattggaa tacatggaat gtgtttgtaa gatatagaat ataactgcat aaatttaaat
    10081 aaaatgaaac agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt
    10141 ttacgttcat gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg
    10201 ttttgtttgg ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa
    10261 aagcccctga tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata
    10321 cagggacaat ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta
    10381 ttgctgtttt ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga
    10441 cttggcgttt ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca
    10501 tactttctta cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg
    10561 cagcttgatt tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa
    10621 aaggtggtag aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg
    10681 ctttcaatgt tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg
    10741 ccctcaaaac ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc
    10801 tgtcttttcg gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg
    10861 ccgttggcta aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa
    10921 tgcgaacttg ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct
    10981 ttgacaaaag gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca
    11041 cgatggcaag atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg
    11101 attgatttcg gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact
    11161 atacagtcat gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg
    11221 tagactgtac actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta
    11281 aagggcatct gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt
    11341 tagcttctta tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag
    11401 aagaacgctt gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt
    11461 cctctcctca gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg
    11521 atatgcgcat ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct
    11581 accatgaatt agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg
    11641 ctcgtgaaat cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg
    11701 aaatcattaa acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa
    11761 gaatttttca agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt
    11821 tagaacaggc aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt
    11881 cattggaaga cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac
    11941 caccgggact tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta
    12001 aaccaatcct tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt
    12061 tacgtattgc agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa
    12121 atgcaagatt acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct
    12181 gattcttctt cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag
    12241 atttctgctt tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt
    12301 gttttaacag ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata
    12361 caaacagaaa tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa
    12421 ctttctcgta ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac
    12481 gacaatttaa ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa
    12541 aagaaaaatc taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg
    12601 agtattggat tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat
    12661 gttgctggta cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta
    12721 aaagcaaaaa gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc
    12781 tcttattcaa ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat
    12841 gttgaagaaa agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac
    12901 aaaaaagatg cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa
    12961 tttggtagct atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg
    13021 aaaaaagaag gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat
    13081 ggcgtctttt cttctcattt tattggatac gcagttccag acaaagatga gaatggctta
    13141 gttggtaaac taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag
    13201 attatttatc aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa
    13261 aaagctgtcg acggacaaga tatctacacg actttagaca gcagactaca aagttattta
    13321 gaaacactga tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg
    13381 atgaaagcaa aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa
    13441 acaatggaag gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt
    13501 tatgaaccag gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa
    13561 ttcaacgaga atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat
    13621 gaccatgact ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt
    13681 aacgtcggga tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa
    13741 aaactgggat ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta
    13801 cctacattaa atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg
    13861 aatttccaaa tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct
    13921 cgatatatca gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa
    13981 gttctagggc aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat
    14041 gttgtcgaaa gcgaaaacta cggtattgct tatggtgtat atagtgtacc tggatacaat
    14101 gtatcggcaa aaaccggaac agcccaaatc gcttctgata cagggggata tcaaacagga
    14161 gatacagcct acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc
    14221 ttgtatctaa caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca
    14281 aatccattga tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa
    14341 acaaaaacag aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca
    14401 gctgatgctc aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa
    14461 aaacaatcta ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact
    14521 ggtggtgata agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg
    14581 gggaaaatcc tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa
    14641 cttgctccat atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa
    14701 gagaaatagg agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag
    14761 ttgtgcaatg acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca
    14821 aggtcaggcg attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac
    14881 aatgggcggc ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg
    14941 gcaaaaacag ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt
    15001 aatcggcttt cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc
    15061 gaagcagaaa ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc
    15121 tgaaggttat ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta
    15181 tggtattttc gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg
    15241 catcgatgga ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc
    15301 ttggcatcag catcagtatg atgtattaat catttgctta agtgttttag gaggtttact
    15361 gggtttcttc gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt
    15421 agcgttgggc ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct
    15481 tttgattggt ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt
    15541 taagcttaca ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg
    15601 cggatggtcg gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt
    15661 gatcacatta tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg
    15721 aaaataagaa agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat
    15781 tacatgaatt gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg
    15841 aagcacaaga gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat
    15901 tgttggatga agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt
    15961 tagtagcaaa agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg
    16021 aagtagcgga atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa
    16081 cgatgacagg actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta
    16141 atattgggta tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga
    16201 tggaattgtc tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga
    16261 ttaccaatat ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag
    16321 ctaaatggaa ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc
    16381 agtcagaact acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa
    16441 aagaagtgtt ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa
    16501 aaataatgga aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag
    16561 ctgctatttc tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc
    16621 attttttcca tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat
    16681 tttataatga ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg
    16741 aaacgtctaa ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat
    16801 taattccttc attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt
    16861 tgatggatgc tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac
    16921 aagcagttcc tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag
    16981 cgaatgccag ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag
    17041 ctgtgaaccg attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt
    17101 acaggcggag gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag
    17161 actcaagaac cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa
    17221 atcgtccctg atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa
    17281 atttccatgc ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa
    17341 aaaatcctca gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga
    17401 gcggtagtgt acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt
    17461 gtaccaggtg ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt
    17521 aaagatgcgg cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca
    17581 caagaagtag cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa
    17641 aagaaaacag tcttgatttt tggcggtagt caaggagcaa ttaaaatcaa tcaagcagta
    17701 tcagtttttt taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat
    17761 agatattatg aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag
    17821 ccttatatca aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct
    17881 ggtgcaactt cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc
    17941 ccatatgtta cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca
    18001 gcaaaaatga ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa
    18061 atcatgggtg atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc
    18121 cctgatgctt caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg
    18181 tggtcttagt gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa
    18241 acaatttgac tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa
    18301 accaagagcc aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag
    18361 aaaaaacggt tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt
    18421 ttgaagagat caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc
    18481 tgcccaattt aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca
    18541 ctgtattggg tataccgctg atttttttga tctattatgt ttctccttat agcaaattac
    18601 aagcagttgc agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa
    18661 aattgtcatt aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc
    18721 gccttaaaaa agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg
    18781 aattcgattt ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat
    18841 actacccagt gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa
    18901 atctccctat cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat
    18961 acaatcagtt atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag
    19021 caagcaataa aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata
    19081 tcaataatct tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa
    19141 aaggtgtgat cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa
    19201 cggaagaatc gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa
    19261 acgaatcaag tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa
    19321 acggatcatc tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta
    19381 caactccgtc aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt
    19441 tctaaaaacc agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat
    19501 attttaaaac ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc
    19561 attcatggca aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt
    19621 tgtcgtggct gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc
    19681 agaagggatt aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg
    19741 agcagtacga caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt
    19801 gccagcaaac atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc
    19861 aaaggaaatc accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat
    19921 acctcctgaa cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga
    19981 gggaatcaaa gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt
    20041 tacaggacct aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat
    20101 cgtcaacgaa atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga
    20161 aaaagatttt ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat
    20221 gcatgataag caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa
    20281 agatatctct attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta
    20341 cggggatgca tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg
    20401 tcagtcagaa ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat
    20461 ggaacagatt ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc
    20521 aggtggcgtc gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca
    20581 agaaatcttc ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc
    20641 ggtatttaca aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca
    20701 attggcaaaa attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca
    20761 agaaacaaca aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc
    20821 ggaaccaaaa gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat
    20881 ttttgattaa ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca
    20941 atgacggcgc agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc
    21001 gaatgattga agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag
    21061 cattaaaaaa ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt
    21121 taggtgctgg ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct
    21181 tacgcgaagc tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa
    21241 ccggtacagg tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg
    21301 ttggtgtcgt gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag
    21361 aaggtatcgc tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt
    21421 tattagaagt tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg
    21481 ttttacgaca aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc
    21541 ttgactttgc tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg
    21601 gtgtggctag tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat
    21661 tgcttgaaac atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg
    21721 atatgacatt gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg
    21781 atgtaaatat cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga
    21841 cggtcatcgc aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta
    21901 gacaagcaca aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac
    21961 caacttcttc agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg
    22021 ttcgtcctag agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat
    22081 tcaaccgcga agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc
    22141 gtcgtaaaaa ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag
    22201 caagaaattc aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta
    22261 gctgtaacaa aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg
    22321 aattgtgcag aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt
    22381 cctgaaataa agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta
    22441 aatgaaattg attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag
    22501 cggttagaga aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa
    22561 catggaattt caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa
    22621 atcaaggttg ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat
    22681 aaagtgtttg gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat
    22741 gcaccatgta gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa
    22801 ggtgcaacat ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa
    22861 aatgccgctt ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga
    22921 gtacgaatac caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa
    22981 tacaacacag aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa
    23041 aacggcgact tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt
    23101 aattgaacta catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac
    23161 ctctacacaa cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta
    23221 ttctgaagct atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt
    23281 tcatttagta gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta
    23341 tgcactagat ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg
    23401 gatggagatt gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta
    23461 agaggaggaa ctacgattta tactattgca ctattacttc atttacttca acgggcagcc
    23521 tatttctata gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat
    23581 caatcagggc taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat
    23641 cgtctgcctt tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg
    23701 aacttagccg tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat
    23761 tgattgaagc aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca
    23821 aatgtatacc agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg
    23881 attgaacagg ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa
    23941 tcctatattt tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc
    24001 ggatatgaac aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta
    24061 gaggaagatt ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta
    24121 agtcatggga aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc
    24181 gatatcatct cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt
    24241 gttgctgcac aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac
    24301 acagaaatga ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg
    24361 cgactagaca gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg
    24421 atcgaagctg gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt
    24481 gaactattag atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag
    24541 gggaagacga aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa
    24601 aaggtggttt actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa
    24661 aaatgcgggg atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact
    24721 atgaggaatt gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa
    24781 aacttgagta cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag
    24841 atacggctga taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg
    24901 cacaaaacaa agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa
    24961 ctgatgcaga agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc
    25021 ttgcgacaga aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct
    25081 tgatgcttga gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac
    25141 cattctccag ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc
    25201 ttgacaatga aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga
    25261 cggcatttga tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt
    25321 agaacagaaa agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag
    25381 acactgcatg gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag
    25441 cagcggctga cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt
    25501 gggtggtacc acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa
    25561 caaataaagg agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa
    25621 aattccctat gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag
    25681 aaaatcgaat ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc
    25741 atgatggacc tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca
    25801 gtaaagacat tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag
    25861 gatgggatac acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta
    25921 aagaaatgtc catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag
    25981 ataagcaacg agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg
    26041 ttacattaga tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa
    26101 aaagctatat ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt
    26161 tagcggaagc tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca
    26221 atgtcaaaga cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa
    26281 caccatggac attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat
    26341 tggtggaagc cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat
    26401 cagaaatcgg ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat
    26461 atatgactgc aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg
    26521 ttactttaga cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact
    26581 atatcgctgg tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt
    26641 ttacagaaga agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca
    26701 ctgatctatt aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc
    26761 ctcacgattg gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat
    26821 ctatcgataa attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc
    26881 catggggtaa aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc
    26941 gtcagcgtgc atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca
    27001 tcacaccaga aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct
    27061 ggtttgaaaa agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta
    27121 atggcgaatt tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc
    27181 atgaagctgt tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag
    27241 gatcagacca atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg
    27301 gtgtagcacc atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta
    27361 agatgagtaa atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg
    27421 cagatatcct tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta
    27481 tggatatctt aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct
    27541 tattagcgaa tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac
    27601 gttctgtaga caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag
    27661 aagggtacga caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg
    27721 ttgatctatc gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag
    27781 atgattatca acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa
    27841 aattattgac tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag
    27901 aagaggagta cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag
    27961 aattgatgga tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg
    28021 aagaagcaag aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc
    28081 caaaacaaca agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga
    28141 tcgtttcacc agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta
    28201 tggtatttga tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct
    28261 gtcgccaagt tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt
    28321 gtgcaaaaat cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt
    28381 aaaagggaaa agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt
    28441 aattttccac gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt
    28501 tcaagtagtt cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc
    28561 ggttgtgttt ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact
    28621 tcttcccaat gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg
    28681 taagcttctg ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt
    28741 tccgtttcaa atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt
    28801 ggctgaatat agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg
    28861 acagatgttt tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct
    28921 ttttcagtca aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta
    28981 tcgatagtga atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct
    29041 tcttcgcgat agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga
    29101 acgaagtttt ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc
    29161 ttttctgtac ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct
    29221 acaacctgta aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag
    29281 cccccgcgat cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag
    29341 cgattgttcc attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg
    29401 atcatttctt ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct
    29461 ctgatttgct ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg
    29521 atcaaacgat tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc
    29581 gttccaaaaa attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag
    29641 tgtccatcta atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca
    29701 tcatgggatt gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc
    29761 aatgcacgga ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga
    29821 gcggttccga ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa
    29881 gacgggtaca atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca
    29941 tttttttcat tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg
    30001 ccgccttaaa gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat
    30061 aagctgcgat gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt
    30121 aatccaacaa ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat
    30181 aggactccaa tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc
    30241 ctccatgagg acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt
    30301 ccgaagtcac atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta
    30361 aatgttcaac taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt
    30421 gcagtccgct tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt
    30481 tcacaagttt agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga
    30541 tttgtttatt gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat
    30601 cttctcgatt tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag
    30661 tgtaacaaaa aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac
    30721 gtttttacaa caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat
    30781 atatatatac attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag
    30841 attcgtcata aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac
    30901 gtacgactaa acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga
    30961 tcaaaaacgt gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga
    31021 accatgtacc ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca
    31081 agtaaataaa acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta
    31141 tcaccaaatc actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt
    31201 cattgtaccg aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt
    31261 ttctacaaat tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag
    31321 caagtggaaa tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa
    31381 aaagacggag aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg
    31441 ataccaggcg ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat
    31501 gatctgattg ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat
    31561 cggctggcaa ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca
    31621 tttacggtat acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag
    31681 acggtggtcg gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg
    31741 attggtgctt ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg
    31801 tatgccgcac aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt
    31861 catctggcaa cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg
    31921 cagcaaagcg gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact
    31981 gtcaagcagg ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa
    32041 gaaggtaaaa taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt
    32101 gtccaagcag ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg
    32161 gaaaaagcac aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg
    32221 aagacttttt tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt
    32281 cactttagtc aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca
    32341 gctttttcga cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg
    32401 tatttttttg aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac
    32461 ttaccattaa aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc
    32521 tatgaccaag tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt
    32581 gcattgatgg atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca
    32641 aaagaagtcc attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg
    32701 atcttacgtt atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat
    32761 ttaccgtcag agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt
    32821 gaacaagcac aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct
    32881 gcagaaaatc caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa
    32941 gatccagctt ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa
    33001 gtgatttatg atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat
    33061 ttttaatgaa cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata
    33121 aagggtttat ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac
    33181 aaagtatagt agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc
    33241 tactactaaa cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag
    33301 aactagctat tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag
    33361 tccaatcttt ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac
    33421 cagaaatctt gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa
    33481 aagcacatca agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga
    33541 acagccaaat cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt
    33601 tttcagcgac agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg
    33661 agacaattga cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac
    33721 gtgtaccgat acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc
    33781 atggtctgat tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt
    33841 atagaggtgt accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag
    33901 cgctagaaca attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta
    33961 gaggtttaga tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag
    34021 aagcttattt gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta
    34081 cactcgtaca agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa
    34141 cacttgaaga gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag
    34201 atgcagctcc tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc
    34261 cagaatataa caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa
    34321 ataaaggagc aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa
    34381 aattggttgc cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt
    34441 gacagaaaac caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg
    34501 aagcaatgca agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc
    34561 tggccgacca gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt
    34621 cgactcttct acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt
    34681 cagcatacag caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat
    34741 ttttggagaa actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat
    34801 cgattcagag atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatgc
    34861 tcaaaatccg caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg
    34921 agcagctcaa gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga
    34981 tgagatccac cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg
    35041 tatttaccgt ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg
    35101 ggcgacgact gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc
    35161 aatcaccttg ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct
    35221 ttttatcaaa caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat
    35281 tcttgccttc atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa
    35341 actagtctgt gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat
    35401 tcgaaaagaa gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc
    35461 ggaacgtctc gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt
    35521 tgctgatgtt gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga
    35581 ggaaaaagtc acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat
    35641 ttcaaaagat gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat
    35701 tcccaaagat cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc
    35761 tgaaaaacgg ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct
    35821 gcaaagtaca ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa
    35881 cgtacggaaa aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga
    35941 agaagaagaa caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc
    36001 tatcaatcta ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat
    36061 gcacggcaga cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa
    36121 agtagggaaa gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt
    36181 gttacaatat gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga
    36241 tatgggatca ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa
    36301 agctatcacg atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg
    36361 aagaagctta gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga
    36421 tcagttcaaa gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg
    36481 ctttactggt gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca
    36541 aaataaggta gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat
    36601 cgatgcctta ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta
    36661 tcaaaatatc ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt
    36721 aaagcgtata gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc
    36781 attaagcgat gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca
    36841 gatccagacc gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca
    36901 tttagccttc atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact
    36961 gtctattttc tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc
    37021 actagaacga aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat
    37081 gtttcttgaa aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca
    37141 tctgtgtgcg cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt
    37201 tttattggca tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa
    37261 tggcaaaaaa aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca
    37321 caaaaatgca aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg
    37381 cctcagatgc agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc
    37441 aagtacgttt catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag
    37501 atgtcatcaa tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag
    37561 aaaatctaat gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca
    37621 aaatctagaa gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat
    37681 ggaagcgatt gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga
    37741 tgcagaagaa tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc
    37801 acaaggaaat catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac
    37861 atctattgcg tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag
    37921 cgaataaata caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat
    37981 tttgttataa ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac
    38041 ggactatcaa gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa
    38101 aaacatttag ttgctttgcg tgatgctttt atcggcacac tgcccgcaac aatggcggga
    38161 tcagtcgctg taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc
    38221 tatgctgaca gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga
    38281 atcaatggtt ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct
    38341 tgggggtata atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt
    38401 ttagctacct tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg
    38461 aatgttcctt caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca
    38521 gatggcacac tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac
    38581 gcttatttta cagttatgat catgggagct atttcagtca ttattttttg taaattgatg
    38641 cttgccaaca ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt
    38701 gcagccattt taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta
    38761 ggtcgtgtag ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagaacca
    38821 ttcctaggat tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta
    38881 ttctggttct ttggtattca tggatcaaat gtattggcac cagtgctaga gggaatctgg
    38941 ggacaagcac aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt
    39001 acaccagccg tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg
    39061 ttcgatgctt ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc
    39121 ttgattttct ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt
    39181 atcttcaata tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg
    39241 tttatcccat tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat
    39301 cttggcctag tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg
    39361 tcattcttag caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc
    39421 gtgactttct tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca
    39481 ggcgaacaac aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat
    39541 gaattttcga ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat
    39601 caaagaattg ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca
    39661 aggaatcaag ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc
    39721 aaaggaacta atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt
    39781 ttagaaaatg gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg
    39841 tagtattggt tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt
    39901 gtatagtatg gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg
    39961 gaaaaaaaga gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat
    40021 attccagcat cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa
    40081 gcgaccggca aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc
    40141 tcgcaaaata accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag
    40201 tttcgtacat cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac
    40261 gattcgacct gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag
    40321 ttgaatgata tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa
    40381 atgctagata tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt
    40441 gaaaaatgaa gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc
    40501 tcactagctt ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg
    40561 tatcttcgat tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac
    40621 ctgaaaggcg agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg
    40681 atgaggagga tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg
    40741 gacaagagta cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac
    40801 aaggcgggat caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa
    40861 gcttatctta tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat
    40921 catttgataa aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat
    40981 ctggaaaaat aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg
    41041 aacagatgcc ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata
    41101 aattcaagca aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa
    41161 cgggatatat cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt
    41221 tggatcatga tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg
    41281 aaacatttga atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc
    41341 catttgcttc tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt
    41401 ttttggaaaa ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag
    41461 atcattatgc gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc
    41521 atctctcaaa agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca
    41581 cgcagctatt attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg
    41641 tgacaaaaga atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta
    41701 atgactattt ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt
    41761 tcatttggca tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt
    41821 tttgttttct tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat
    41881 cttttcccga ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt
    41941 atcattcatt ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt
    42001 taaggactag tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt
    42061 gcaagtaaaa aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag
    42121 gatagagcaa tcgcctccta agcgataggt cgctggttca attccagtca ggaacgtaca
    42181 aataacccct gtatcctttg cggtacaggg ggttattttg tataagtaga cattgtagtt
    42241 tctaaactgc tatgtcctaa acgtttcgat acgctaagta tatttactcc ttgataaagt
    42301 aaaatagatg catgcgtatg tcttagtcca tcaataaaca gtcgaagaga aaatagggaa
    42361 aaattgatta ctaaagaaaa acttactagg tcgacggtgt agttgatttt catacttcaa
    42421 tagattcgaa ttaaacgatt tcgattggct tataccagtc ctatccaacg aaagatatat
    42481 cgttcttaac tctagcggta taacttgaag ctttacgtaa agccaatggg aatgaactgg
    42541 agatgacttt ttattatttt tagtaatagc tgatgggtta atacttacaa ctttcctttt
    42601 caaagtacca tataaaaaaa cggtggggtt gtttagcgaa ataaaggatc taagccaata
    42661 gcttagatcc tttattctat gctttatatt attttttgca tgtgtgcgaa agtatactca
    42721 agcccaatcg ccatttcgga aaatcggtac tgtagagcca tcttcacgga ttccgtcgat
    42781 atccatcttg tcagatccta ccatgaaatc tacgtgggtt tgacttctat tcaatccagc
    42841 ttctgctaat tcttcatcgg acatttctgt accgcctttc acactgaagg cataagcaga
    42901 acctagtgct aagtgattcg atgcgttttc atcaaataat gtattgaaaa agatgatgcc
    42961 agattgagaa ataggtgaag ggtcaggtac aagtgcgact tctcctaaac gacgcgcacc
    43021 ttcatctgta tcaagcagtt ttgctaagac atcttctcct tgttcggcag aaaaatcaac
    43081 aacttttcca tctttaaaag taaatttcat acccgagatg atggtgccag cataactcag
    43141 tggctttgta cttgaaatgt aaccatctac tcgatgactg tcaggtgctg tgaacacttc
    43201 ttccgtcggc atatttgcca tgaatttttc tccacgagca ttatagcttc cagcgccttc
    43261 ccaaagatgg tttttaggca atccgataat gatatctgta ccgggagcag tatagtgaag
    43321 ggcagaaaat tgttctcgat tcaattcttc tgcttttttt gctaattttt catcatgctt
    43381 cttccatgct aagacagggt cttcttcata gacacgagtc gttttgaaaa tctgatccca
    43441 taatgcctca acttgttttt cttctggcag ttctgggaag acttttgctg cccattgttt
    43501 accagccgca gccacgacag tccaactgac tttatttgct tgagttgcct tgcgtaaatt
    43561 cattagtgct ttgccattcg ctgattgata agacgctact cgatggctat ctacaccagc
    43621 aaatgcatcg ggatcggcag aaacaacgct gatcctgctt gctccttttt caagccattc
    43681 atccgcttga tcgattttag attgtggtac attctcaata cgatccgttg cagcatgtaa
    43741 aagaaattct ctttggatct ggtcgtcagt ccattgaacg ataacctcag cggctcctag
    43801 tttataagct tcttgtgtga tcaatcgagc aaggggtgcc tgttccacgc tgatctgtaa
    43861 aacaactgtg tgtccttttt ctgtggctac gccagtttct gcaattaagc gtgcatattt
    43921 ttttagaagt tcattaaaat cagataacat aataaaaatt cctcctcacc attttttctg
    43981 gtaactatta gcatgatacc atttaattat gattcaaaca atgaggcttt aaataaaaaa
    44041 aggaggttat ttttttaagg ttaagtttca tgatatatgt tactacttgc tatttcagct
    44101 tgtgaagaaa atcaaaaact aattttgcat atcaaaaaaa catgttacaa tgaaattaat
    44161 caatggtgga agcgttataa aaaaattaag gagattaaaa aatatggcaa gaaaaaagac
    44221 gattactaga gaacagattt tggcagctgc ttatgaagta gctgccaccg atggatttac
    44281 acgatttact gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac cgatttatct
    44341 tgagtttaaa aatatggatg acttgagaca agctcttttt gagcagatct atgatcattt
    44401 acaatatgaa gtgttcactg ttgagcatac agggaatacg attatcgatc ttgcttttaa
    44461 ttatatccat tttgcaagaa gagagaagaa gttgtatcgt tcactgtact tagaagaata
    44521 tggtggagga aaagagatgc agaatttctc ttatacttac tttgtgaata cagtgaaacg
    44581 cgatccagct tatgtgaatc tttcagaaga gcagatcgaa tctttgtata atgggacatg
    44641 gatcatagct actgggatcg cagctttgat gtcttcaagc atcatccatc caactgatga
    44701 acagattgaa aagatgatcc aagacagtat agatgctatc ttgaaattca aagaaccaat
    44761 cgaaatcgat tgaatatact caaaagttcc acaaatcagt aggtaacaag agatgtgtca
    44821 aaataaggaa aagttcgttt ttcttacagc gtatgcattt ttaactcccg aagaactctt
    44881 ccatgaatgc aatgaataaa agagccatac agattgaata aatctggatg gctcttttat
    44941 tgcaagtata ttaaattttt gcgactagtt ctttagcgaa agcttctaaa ttttcgatat
    45001 cttcttcttc agcattcaga tccactttaa cattttcggc tccttttgat gcgcctgttt
    45061 ttgcaaaagc agcatcgaaa tcatccaccg atttgcagaa ttcatcgtaa aaagtatcac
    45121 cagatccgca gacaccgtaa atttttccca atagatccaa ttcttgcaag tcttcataaa
    45181 agtctacgat ctcatcaggc agatcgccat catcgtatgt ataagtagct actacgcaaa
    45241 tatctgcctc ctcgaattca tctgcatcta cttgggtaca ttcgttgatt tcgacttcga
    45301 tatccagatt ttcaaatgct tctgcaacaa tatcggcgat ttcttcagtg tttcctgtca
    45361 tgctagcata tacgattttt gctaaagcca tgatgtgcct cctcgtttat aattatttaa
    45421 caattatgaa ttataccaaa acatcattga aaagaaaata aaacctaaag acacctgggg
    45481 aatgtcattt ggctaaatgt atgccgctat gttaaaatga gagggatttg taaaaaggag
    45541 acgaaaatat gattacttta aaatcagcac gtgaaattga aatgatggca gaatctggag
    45601 ctttattagc agatgtacat aaaaatctgc gtgattttat aaaaccagga atcacaagtt
    45661 gggatattga ggtatttgtc agaaactata tcgagagtca tggcggaatt gctgcgcaaa
    45721 ttggctttga agggtatgaa tatgcgactt gtatcagtat aaatgatgaa atttgtcatg
    45781 gattccctcg taaaaagcct ttgaaaaacg gtgatttggt aaaagtcgat atgtgtatcg
    45841 atttgaaagg cggcatttct gattcttgtt gggcctatgt agttggagaa tctacacctg
    45901 agatcgaccg attgatggaa gtaacgaaga aagctttgta catcggtatt gagcaagcac
    45961 aagtagggaa tcggattggt gacatcggtc atgctatcca aacttatgta gaaggcgaaa
    46021 atctagcgat cgttcgtgat tttattggtc atggcgttgg tcctacgatt catgaagaac
    46081 cagcagttcc tcattacgga gaagctggaa aagggttgcg gttgaaagaa ggcatggtta
    46141 tcacgatcga acctatggtt aataccggaa cttggaaaat gaaaatggat cctaacggct
    46201 ggactgcgta tacaagagac ggcggacttt cttgccaata tgaacataca ttggctatta
    46261 ctaaagatgg tccaagaatt ttgacatcac aaggagaaga aggcacttat tgatctatcc
    46321 agtcaatagg tacgggagag gtacatgaaa gtttggaaca aaataaaaca aaatgaaaat
    46381 ctgatgcgct ttattgagac gactcaaaag cgcatcactg attcagaaat gagtacgacc
    46441 tctgtcgtcg ttgcttatta tttactcttg tctttatttc cattgattat tgcttttggt
    46501 aatattttac cgtttctcca catcgatcaa gagactgtac tgacctattt acgagaagtg
    46561 attcctgaga ccgtttacca gtttatcggt cctgcaatca aagacctttt gacacaaagt
    46621 tcaggaggct tgttatccgt ttctgcatta gcggctttat ggtctgcaag tcaaagtatc
    46681 aatgctttgc aaattgcgat gaataaagcg tatggcgtag aaaatagaaa aaattttttg
    46741 atcgtccgtt tcttctcact aattgtcatt gtcttgttca tgattgctat tagtggggta
    46801 actatcgttc ttgggttggg acagctgatt ttggatgcta ttcagccaat cgtacaaata
    46861 cccgtgaact ttatcgatca gttccaaact ttgaagtggc caatcacatt ggtagcttta
    46921 tttgtcatca tgttcttgat ttatttaatt gtccctaatg ctcagttgaa gttgaagacg
    46981 atcattccgg gcacgatctt cgctactgtc ggctggatgc tgttatcaca agtgtttagt
    47041 atctatgcta ggtattttgc aacgaggatc agcggttatc agatcatagg aagttttatt
    47101 gtactcatgc tttggcttaa ttttgctgct acgatcatca ttttgggcgg aatcatcaat
    47161 gcagttgttc aagaatacgt gacgggtcat caaatcaaaa aacgtcgaga tccaagccga
    47221 aaatggttca ataaattgaa aactaaattt tctcataaaa aaaagtagaa acaagagttc
    47281 ttattgtaaa tttatatcag taatttataa taattatgac tcttaattaa agtaggtgcc
    47341 catatgcttg tttcttttag tat
//
LOCUS       DAYYNG010000003        44552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000003 DAYYNG010000000
VERSION     DAYYNG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.003"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..534
                     /locus_tag="ACHBFA_000120"
     CDS             34..534
                     /locus_tag="ACHBFA_000120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9393649.1"
                     /translation="MTFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQ
                     GNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPG
                     VVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRR
                     RTQKFL"
     gene            662..1345
                     /locus_tag="ACHBFA_000121"
     CDS             662..1345
                     /locus_tag="ACHBFA_000121"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9393650.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1654..1727
                     /locus_tag="ACHBFA_000122"
     tRNA            1654..1727
                     /locus_tag="ACHBFA_000122"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1688..1690,aa:Arg,seq:tct)
     gene            1886..2245
                     /locus_tag="ACHBFA_000123"
     CDS             1886..2245
                     /locus_tag="ACHBFA_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9393651.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2368..3999
                     /locus_tag="ACHBFA_000124"
     CDS             2368..3999
                     /locus_tag="ACHBFA_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM9393652.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4061..4702)
                     /locus_tag="ACHBFA_000125"
     CDS             complement(4061..4702)
                     /locus_tag="ACHBFA_000125"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9393653.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4888..4964
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4984..5292
                     /gene="rplU"
                     /locus_tag="ACHBFA_000126"
     CDS             4984..5292
                     /gene="rplU"
                     /locus_tag="ACHBFA_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9393654.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5319..5663
                     /locus_tag="ACHBFA_000127"
     CDS             5319..5663
                     /locus_tag="ACHBFA_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9393655.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5676..5969
                     /gene="rpmA"
                     /locus_tag="ACHBFA_000128"
     CDS             5676..5969
                     /gene="rpmA"
                     /locus_tag="ACHBFA_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9393656.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6062..7210)
                     /locus_tag="ACHBFA_000129"
     CDS             complement(6062..7210)
                     /locus_tag="ACHBFA_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9393657.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQAVAERYNVYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7222..7533)
                     /locus_tag="ACHBFA_000130"
     CDS             complement(7222..7533)
                     /locus_tag="ACHBFA_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393658.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7586..7981)
                     /locus_tag="ACHBFA_000131"
     CDS             complement(7586..7981)
                     /locus_tag="ACHBFA_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9393659.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8017..8364)
                     /locus_tag="ACHBFA_000132"
     CDS             complement(8017..8364)
                     /locus_tag="ACHBFA_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9393660.1"
                     /translation="MTTFERVKMLADKRKISIVELEEKLNFSKNSLYAWKKSKPSIDK
                     LNAVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREIL
                     SAMIEAYLEKKDN"
     gene            8662..8883
                     /locus_tag="ACHBFA_000133"
     CDS             8662..8883
                     /locus_tag="ACHBFA_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393661.1"
                     /translation="MKQNKKTPSAKGALITIHFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGNLF"
     gene            9009..9785
                     /locus_tag="ACHBFA_000134"
     CDS             9009..9785
                     /locus_tag="ACHBFA_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9393662.1"
                     /translation="MNTPQIFNFGQQEVRTVLLNDEPYFVGKDVAEILGYERADNAVR
                     NHVDEEDRLMHRISASGQNRNMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLRNKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEER"
     gene            9788..10000
                     /locus_tag="ACHBFA_000135"
     CDS             9788..10000
                     /locus_tag="ACHBFA_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9393663.1"
                     /translation="MSQDFILKVRVALATHDKSQAWLAEKLGISTAYMSDIMNGRRKP
                     DKQIKPIEAVLAELEKEKNYANNNSR"
     gene            9978..10262
                     /locus_tag="ACHBFA_000136"
     CDS             9978..10262
                     /locus_tag="ACHBFA_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393664.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLEERLHFMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWINLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10283..10471
                     /locus_tag="ACHBFA_000137"
     CDS             10283..10471
                     /locus_tag="ACHBFA_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393665.1"
                     /translation="MSYTLQQEHQILRLIKQRRKQLQDDREALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10468..10641
                     /locus_tag="ACHBFA_000138"
     CDS             10468..10641
                     /locus_tag="ACHBFA_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393666.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            10610..10738
                     /locus_tag="ACHBFA_000139"
     CDS             10610..10738
                     /locus_tag="ACHBFA_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393667.1"
                     /translation="MAREEALKIGKVIADNWWTNNRPIILSKQHIEKQKAWQQIKK"
     gene            10794..11135
                     /locus_tag="ACHBFA_000140"
     CDS             10794..11135
                     /locus_tag="ACHBFA_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393668.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            11128..11799
                     /locus_tag="ACHBFA_000141"
     CDS             11128..11799
                     /locus_tag="ACHBFA_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9393669.1"
                     /translation="MSEIKGITNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            11805..12491
                     /locus_tag="ACHBFA_000142"
     CDS             11805..12491
                     /locus_tag="ACHBFA_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9393670.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRSISLK
                     DESSSTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            12494..13324
                     /locus_tag="ACHBFA_000143"
     CDS             12494..13324
                     /locus_tag="ACHBFA_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM9393671.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLGSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSNEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            13341..14192
                     /locus_tag="ACHBFA_000144"
     CDS             13341..14192
                     /locus_tag="ACHBFA_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9393672.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDLQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            14189..14548
                     /locus_tag="ACHBFA_000145"
     CDS             14189..14548
                     /locus_tag="ACHBFA_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9393673.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            14563..14724
                     /locus_tag="ACHBFA_000146"
     CDS             14563..14724
                     /locus_tag="ACHBFA_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9393674.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            14721..15032
                     /locus_tag="ACHBFA_000147"
     CDS             14721..15032
                     /locus_tag="ACHBFA_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393675.1"
                     /translation="MSVDNKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK
                     C"
     gene            15026..15316
                     /locus_tag="ACHBFA_000148"
     CDS             15026..15316
                     /locus_tag="ACHBFA_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393676.1"
                     /translation="MLDMKIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            15316..15633
                     /locus_tag="ACHBFA_000149"
     CDS             15316..15633
                     /locus_tag="ACHBFA_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393677.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            15630..16445
                     /locus_tag="ACHBFA_000150"
     CDS             15630..16445
                     /locus_tag="ACHBFA_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM9393678.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            16584..16898
                     /locus_tag="ACHBFA_000151"
     CDS             16584..16898
                     /locus_tag="ACHBFA_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM9393679.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGSGVR
                     TSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            17075..17872
                     /locus_tag="ACHBFA_000152"
     CDS             17075..17872
                     /locus_tag="ACHBFA_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9393680.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLKQL
                     DEPKKLVIPQLVAGWLEKFTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKSKWVVNEGDLVICKGKHEAKVYFVESVDDDGI
                     LLVNGIKDEFFTDLDDRSVDEESINYFYENFRLLAKKENLEAEKVEV"
     gene            17874..18062
                     /locus_tag="ACHBFA_000153"
     CDS             17874..18062
                     /locus_tag="ACHBFA_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM9393681.1"
                     /translation="MNITEEAEYFIELPAKQNKLYVNYDIEENISIDAIRVTRFTEAE
                     IKAIDERYWPFAVPVEKV"
     gene            18073..18363
                     /locus_tag="ACHBFA_000154"
     CDS             18073..18363
                     /locus_tag="ACHBFA_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393682.1"
                     /translation="MIKKIKTLIDGFLLERKLVKVRELIKIHIDSGERSMYWVATDSE
                     KQNVMNMIRFFEIAFEDGYFATGEYFDASSWMSSNPEEVWQIYLEMKEEAEG"
     gene            18432..18626
                     /locus_tag="ACHBFA_000155"
     CDS             18432..18626
                     /locus_tag="ACHBFA_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393683.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            18657..18869
                     /locus_tag="ACHBFA_000156"
     CDS             18657..18869
                     /locus_tag="ACHBFA_000156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393684.1"
                     /translation="MNKKELTVEKEPKYIVKIGKLYLKEPLGDTSNFTILTTWDKKCA
                     YPFSSFHMADIHAAKFEGTVEEVAEG"
     gene            18866..19252
                     /locus_tag="ACHBFA_000157"
     CDS             18866..19252
                     /locus_tag="ACHBFA_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393685.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLES
                     EDLRQIEKSIQLNDNQQIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLAAFAQWGLEQEEAE"
     gene            19249..19449
                     /locus_tag="ACHBFA_000158"
     CDS             19249..19449
                     /locus_tag="ACHBFA_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393686.1"
                     /translation="MNYKVGWWKKGEQYRYCLGMGLDGLVYYRTKRQVLGNSRCITGR
                     HPDFDKWFEKAEYVGLELEDTE"
     gene            19446..19655
                     /locus_tag="ACHBFA_000159"
     CDS             19446..19655
                     /locus_tag="ACHBFA_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393687.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQAYELVTVDHEEVER"
     gene            19652..19951
                     /locus_tag="ACHBFA_000160"
     CDS             19652..19951
                     /locus_tag="ACHBFA_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393688.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVARLVDMEVINNGI"
     gene            19941..20099
                     /locus_tag="ACHBFA_000161"
     CDS             19941..20099
                     /locus_tag="ACHBFA_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393689.1"
                     /translation="MEFKIFEEDTRYELEEKLNEFAKNNEIQHISLTASKSGCTTYYA
                     AVVSYVSQ"
     gene            20193..20609
                     /locus_tag="ACHBFA_000162"
     CDS             20193..20609
                     /locus_tag="ACHBFA_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9393690.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            20871..21212
                     /locus_tag="ACHBFA_000163"
     CDS             20871..21212
                     /locus_tag="ACHBFA_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393691.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRVTSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(21205..21396)
                     /locus_tag="ACHBFA_000164"
     CDS             complement(21205..21396)
                     /locus_tag="ACHBFA_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFM9393692.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            21672..21809
                     /locus_tag="ACHBFA_000165"
     CDS             21672..21809
                     /locus_tag="ACHBFA_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393693.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            22319..22543
                     /locus_tag="ACHBFA_000166"
     CDS             22319..22543
                     /locus_tag="ACHBFA_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393694.1"
                     /translation="MRNYWYISLTNKYPQPNADDPIRVVRSVQIKKKYSIIEMTREAT
                     PKEVDKYNLRYCGHGYFSEQNIQTNIKKYH"
     gene            22569..23024
                     /locus_tag="ACHBFA_000167"
     CDS             22569..23024
                     /locus_tag="ACHBFA_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFM9393695.1"
                     /translation="MSKLNPKQQAFADEYIITGNAYQSALKAGYKENYAKNAQEKLVE
                     KGGKVSDYIQEKLKEVQTKRHLTMEEALAITASIAKGEPQRFEVVKRDPYTNEIIERE
                     VSEYSAGFKERNQALEHYYKINAAFVDKQKVEISEIPTFIDDISSDDDG"
     gene            23017..24315
                     /locus_tag="ACHBFA_000168"
     CDS             23017..24315
                     /locus_tag="ACHBFA_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFM9393696.1"
                     /translation="MAKKLSEFLPPKFHSVWRATLNQDILNIVCKGGRGSGKSSDIAH
                     IVTQLLMRYAVNAVGIRYVDNTLEQSIYEQMKWAIEKQGVSRLFKFNKSPLKITYLPR
                     GNYMIFRGAQNPERIKSLKDSKFPFAIGWIEELAEFKTEDEVTTITNSLLRGELDDGL
                     FYKFFYSYNPPKRKQSWVNKKYETSFQPDNTFIHHSTYRDNPFISKEFLKEVEATRER
                     NPRRAEWEYDGKAVGSGVVPFDNLQVKKGSITDDMVANFDNIRNGLDYGYATDPLAFV
                     RWHYDKKKNGIYAIDEIYGVKISNREFANKAKSKGYQNEEIFSDSAEPKSNAELVNEH
                     GMKGIKGVKKGPDSVEYGEQWLDDLAFICIDPLRTPNIAKEFENIDYQTDRDGNPKPR
                     LEDKDNHTIDATRYAFNEDMWAKKKSTVTKEQRNKIRRMF"
     gene            24328..25827
                     /locus_tag="ACHBFA_000169"
     CDS             24328..25827
                     /locus_tag="ACHBFA_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9393697.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKDRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQTGIFDSDGEEVIYNKKDETGE"
     gene            25832..26758
                     /locus_tag="ACHBFA_000170"
     CDS             25832..26758
                     /locus_tag="ACHBFA_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM9393698.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLLRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNIQRLMVTETTRVQVGIQERSYRDAGVTQYIYIAEPTACKLCIPLNNQAF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            26885..27487
                     /locus_tag="ACHBFA_000171"
     CDS             26885..27487
                     /locus_tag="ACHBFA_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM9393699.1"
                     /translation="MEKRLFMPMNLQFFSEPGDGGSGDGGQQGNPPAGSQETPPAVKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELEAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            27500..28423
                     /locus_tag="ACHBFA_000172"
     CDS             27500..28423
                     /locus_tag="ACHBFA_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9393700.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDESVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRSA
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            28441..28704
                     /locus_tag="ACHBFA_000173"
     CDS             28441..28704
                     /locus_tag="ACHBFA_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393701.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAATVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            28716..29045
                     /locus_tag="ACHBFA_000174"
     CDS             28716..29045
                     /locus_tag="ACHBFA_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFM9393702.1"
                     /translation="MMIADDIKKLLKGTLDEKLEVIERRTNERMKILLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRLKFI"
     gene            29042..29368
                     /locus_tag="ACHBFA_000175"
     CDS             29042..29368
                     /locus_tag="ACHBFA_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393703.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            29352..29690
                     /locus_tag="ACHBFA_000176"
     CDS             29352..29690
                     /locus_tag="ACHBFA_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9393704.1"
                     /translation="MKAQLEYKGIDQLMRHLKKVATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            29690..30082
                     /locus_tag="ACHBFA_000177"
     CDS             29690..30082
                     /locus_tag="ACHBFA_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFM9393705.1"
                     /translation="MIKTRDQSVFDELFKISQEKLGYKTYDYKPLDNVGYPFVEFENT
                     QTIHEANKTDIKGTVIVVLSVWGLQKKRKQVSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            30093..30605
                     /locus_tag="ACHBFA_000178"
     CDS             30093..30605
                     /locus_tag="ACHBFA_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HFM9393706.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDD
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKVTSTGA"
     gene            30651..31019
                     /locus_tag="ACHBFA_000179"
     CDS             30651..31019
                     /locus_tag="ACHBFA_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFM9393707.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            31073..31360
                     /locus_tag="ACHBFA_000180"
     CDS             31073..31360
                     /locus_tag="ACHBFA_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393708.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            31377..34199
                     /locus_tag="ACHBFA_000181"
     CDS             31377..34199
                     /locus_tag="ACHBFA_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9393709.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIRKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIAIAIGPVLYALGMLVKAFQTMKVGLGVLGNGISLFKKLGSAIGFLTSPVGLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGAENVWTTIQEAPGKAADWIKNKWTETKEFFSSIWDGIKEAASSAWEGIVNILAP
                     YVIAIKNVFQPMIDFFTNLWSQIGSIAGSAWEIIKTAVMGPILLLIDLITGNFNQLKE
                     DASMLWTTLTTNIQNIITTFVDIVVGYYTALKDTVINIWNVLTSTIKDVWNSFTTWIK
                     ETTNNIVNSIKQEWNNLKQGTIDLFNNMIQGAKDLWNSFKAWFINLVIGTKDNIIQGW
                     ENLKQGTIDTFNNLVSGAQKVWDNLVNAVSDTVDRVAGWFDNLKNIDLLAAGKAIMDS
                     FLEGLQNAWKSVQDFVGGIGDWIREHKGPIRYDRKLLIPAGQAIMNGLNAGLTNGFAS
                     VQSNVGNMANMIADSFTRTPDIDLSANLKNANRNFTTQIEHSVNYGKNKRPAVFNIRL
                     GNQVFEAFVEDISNIQGKEADINLLF"
     gene            34214..34999
                     /locus_tag="ACHBFA_000182"
     CDS             34214..34999
                     /locus_tag="ACHBFA_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HFM9393710.1"
                     /translation="MMEWHNPMYEFRDTVKNDSQKTWIPTSALNYDGKFIENYIEGYQ
                     TLYVEGREMVSLEIESEAVSIGVRISSQRLPERILTIHFKLEEKNPIEFQRSFNKLMR
                     LLYRDKDVEIHFNDELDMYYYGRYQTCDNIPGNVHSVISSFSIICSDPRKYTRIFETN
                     GIVSEYLPYEVAPISISLKANNDGSLRITNGRQNISVTNSMIKKGDFIEMDIAEGKVF
                     VNGVNKTRILDLTSSFKNFMVRTGDLVKCDNGTPLIRYRGVWL"
     gene            34999..37887
                     /locus_tag="ACHBFA_000183"
     CDS             34999..37887
                     /locus_tag="ACHBFA_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9393711.1"
                     /translation="MDKDVYFFDDSQKLIKIVGEDRLFSVVQEKEITPSKDELINDKL
                     AVSMEFDNEIKESAYMAVRESESSFSMYKIIGIADPGSLLIFTGINFGPDELDAYIIN
                     DIRPANEFFQKTIQRVIDFTSGEWRVGHLDSTLPAVSMTFYYCSIREALKNLQTLGCE
                     IVFRCNLSGEGITDKWIEVYKQIGEYSNERYEYGDKALTIEKEVDRSNIYTSLIGRGR
                     GEEVGDGYGRRIEFDQVYWSKSKGDPLNKPTGQIYLEIPEMTEKYGIPTKNGKRRKRE
                     KVIIFEDCEDPVELIQLTYQELVNCSRPLVQFKATIFGADSLGNIIRIHRDDRGYHYE
                     TRIFSVKIDRLTGKVETGLGDNLNTSSTRQASNTQTAIQTLDEKKMTFYESTEVSKWQ
                     SDIIRGAKGGSIIMMNPWDTGKGESRQPYQMVWMNGDSIDTSNHFLVANSEGIGFIDG
                     KFNESNFKTAWTIDGNFNANYIQSGRIRADIFETSFNAVGDQLKLVKGALQIVNSNKK
                     IMELTKKGMEFWNAKESIGTIGTTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILI
                     SPKEGKGWVILGNGTSILFGSLNLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQG
                     GSGNNDGGSWNGMYPPEVTSQADKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAM
                     NPDIAQVGGPAYGIVQWDGSAYPLVPPATWDGRTYVQNLMRAAGITEDYRTMSAQGKL
                     LDWTMYNGQWLGIVQPASVSGFKAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYN
                     KFKDLQIPSAGSILSTAKSLMGYFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTK
                     AGYKTATRGTLWYTGSMAADARGPRQYLTEISPNEAKAGDIIIVNQGAGAGNDGHTAI
                     LAEDWKGYSTSIVEMGGMNSNGVGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            37898..38416
                     /locus_tag="ACHBFA_000184"
     CDS             37898..38416
                     /locus_tag="ACHBFA_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFM9393712.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTK
                     YEIKVRHGNVFSAAEIGRAYPWALYLWTVIVHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            38382..39290
                     /locus_tag="ACHBFA_000185"
     CDS             38382..39290
                     /locus_tag="ACHBFA_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9393713.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYKDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMSAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            39290..40078
                     /locus_tag="ACHBFA_000186"
     CDS             39290..40078
                     /locus_tag="ACHBFA_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393714.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NAEVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGDGEGGGVTQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            40083..40451
                     /locus_tag="ACHBFA_000187"
     CDS             40083..40451
                     /locus_tag="ACHBFA_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393715.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTTASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGLANITFEKVGTV"
     gene            40451..40975
                     /locus_tag="ACHBFA_000188"
     CDS             40451..40975
                     /locus_tag="ACHBFA_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393716.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            41003..41410
                     /locus_tag="ACHBFA_000189"
     CDS             41003..41410
                     /locus_tag="ACHBFA_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393717.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNTALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            41410..41547
                     /locus_tag="ACHBFA_000190"
     CDS             41410..41547
                     /locus_tag="ACHBFA_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9393718.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRIFVPLCITDEEADKIISKEESA
                     S"
     gene            41582..41824
                     /locus_tag="ACHBFA_000191"
     CDS             41582..41824
                     /locus_tag="ACHBFA_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFM9393719.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNVEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            41839..42036
                     /locus_tag="ACHBFA_000192"
     CDS             41839..42036
                     /locus_tag="ACHBFA_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9393720.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            42047..43066
                     /locus_tag="ACHBFA_000193"
     CDS             42047..43066
                     /locus_tag="ACHBFA_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9393721.1"
                     /translation="MKKKITITVMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKAIFEKNYKAYVELARDSAMKYGIPLTLDTPYNQLGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQDYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(43176..43724)
                     /locus_tag="ACHBFA_000194"
     CDS             complement(43176..43724)
                     /locus_tag="ACHBFA_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFM9393722.1"
                     /translation="MPKDLKKTYNQYLTNLDGKTAILTTPFTPLDCFYIKFDILQLPH
                     LLGLHKIYNEPPKSLCSKLASSSITYEKIQRHKMFGSIKDRITLFDFILDIFLEDYNG
                     SVIYVSEADRNGSSMKLDIAFNHPYKNKILTLGLREISPAIYIPVTFYVKKKIIAQDF
                     QQSKRAKILSLEFIGTKNILLR"
     gene            44446..44519
                     /locus_tag="ACHBFA_000195"
     tRNA            44446..44519
                     /locus_tag="ACHBFA_000195"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44480..44482,aa:Ile,seq:cat)
BASE COUNT        15403 a         6968 c         9483 g        12698 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaataactt ttataagttc tataacgttg
       61 attatatttt taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact
      121 ccttatatta ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat
      181 gtacgaccga aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat
      241 tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt
      301 tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt
      361 atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa aacggcattt
      421 gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg
      481 aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt
      541 taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa
      601 cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag
      661 tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg
      721 tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc
      781 agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct
      841 tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc
      901 cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga
      961 gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg
     1021 tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag
     1081 tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg
     1141 cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat
     1201 tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga
     1261 tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa
     1321 ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga
     1381 atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga
     1441 tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg
     1501 ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg
     1561 ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa
     1621 ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca
     1681 tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt
     1741 agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa
     1801 aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa
     1861 actcatcaga aaagaggaag aagatatgac gatactatct agaattttag tgactctagt
     1921 tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac
     1981 aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt
     2041 taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct
     2101 ttcttccgca tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt
     2161 atatggcagt ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt
     2221 ggcattgctt tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac
     2281 tttgtgaaaa atattacttt atctcatgaa atatgatatt ctaatgaatg gtattttaga
     2341 aaatttcatg aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc actaaaaaaa
     2401 gaattttcag gttatgatct ttcaagattt cagaaagatc ttttggctgg gatcacagta
     2461 gctgctgtag cattgccttt agccttggct tttggggtat caagcggagc tgatgcggcc
     2521 gcaggtttga ttacggctat cattgccggt atcgttattg gcggtttatc aggcggtttt
     2581 tatcaaatat caggtccgac aggtgcgatg gcagctatct tgatgtctat tgcggcaaca
     2641 aagggaatgc aaggtgtatt gcttgctact tttttagcgg gtctattttt acttgcagca
     2701 ggacttttcc gattaggaac actgacttct tttatcccag cacccgtaat tacggggttt
     2761 acttcaggta ttgctatcat tattgcatta ggtcagatcg ataatttatt tggtgttcac
     2821 tcggaaggta caaacgttat tgaaaaacta agtagttatc aaacgatagg atttccgata
     2881 aacacagcct ctttgatcat gggagggcta gtcattttag ggatgtttat ctatccaaaa
     2941 aaatgggcga aacgaatgcc tagttcgctg ctggctatca ttttggcaac agcagttatg
     3001 ctattgttcc atttaccagt agcaactgtg ggtaagattc cacaaacatt gatcagtagt
     3061 aatcgtttga atatgggaga ttttagcttt tctgccttgc aacaagtggc agttccagct
     3121 attagcattg ctttattagg aatgattgaa agcttgctat gtggtgcttc ggctggccga
     3181 atggcaaata agcccttaga tagcaatcaa gaattaatgg cgcaaggaat tggaaatctg
     3241 ttgcttcctt tctttggcgg gattcctgct actgcggcca tcgcccgtac aagcgtggcc
     3301 atcaaatctg gtgcacaaac tagattagct ggtatgatac atgcagttgt cttactgctc
     3361 tccatgcttg tttttgcgcc agtgatgtca cagattccaa tgccagcact tgcaggtgtt
     3421 ttgattgtga cggcttggcg tatgaatgaa tgggaaacga tcaaggagct atttactaat
     3481 aaatattggt cagcagttct attgtttatc cttacgatga tttgtacagt tatctttgat
     3541 ttaagtattg ctatcgtgat cggggtgatc ggcggctgtg tattttttgt tgtcaaaagc
     3601 gctgctatca cgatttcatt agaaacgatc gattggcaaa gaatggggct gccagaaacg
     3661 gaaaagctga ataactggac agtagtgtat attagtggtc cactgttttt tatgtcttca
     3721 gaaaaactca aaaataaatt ggaagaacta aatcatcacg atggtgtcat tttctccatg
     3781 cgaggcgtac caagtattga tttgacggca cgtgcagttt ttgatgaatt tcaagaaaaa
     3841 gcagagcaaa gaggacaaac gattatctat acttccttgc agccagaagt agaaaaacaa
     3901 ctttctcact tgtgggaaca tacattcacg catcaacatc aaacagttgc tcatgcgttg
     3961 acagctttac atgcgaaata tactgcaatt agccattaaa acaatgaaaa agaacagaca
     4021 agactgtaaa ctgaagattt tatggtcttg tctgttcttt ttaatattgc tgtaaaagtg
     4081 aagctagtac ttgccagtct tgatggataa caggtacaag ggagcgattg tagaaaatag
     4141 ctgctggatg aaaaagtgga agaattcgat agtttttttc tgaccattcg taggcatcct
     4201 tttgttctgt taattgtaag acaggccctg tatagagttg accatgattt ttggtaaccg
     4261 tatattcttt gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca attaattttg
     4321 gctggacctt catcagttcg taatccaata ttggtgcatg cgcaaatact tcggaacgag
     4381 taggtgtccg atttggataa acagtttctg actgttgtgt ccgtttattg atccgatgag
     4441 tcacgcgata cgggcggctc cttacagcac tggtgatata cacctcttct cgtgttaagc
     4501 cagttgaaga aagtgcctgc atcaattctt ttccagcttg accgctaaaa ggaacatgcg
     4561 tatcaatctc tgttttccct ggggcttctc ccacgagcat cagtttagga tgaagaggtc
     4621 cttgcccagc gataaatcct tctacaggac ggccgttcat tcttaaagaa gcttcttgta
     4681 tcagttcttt tgggtattcc atttatgctc ctcctttttc ttttattata agaataaaag
     4741 aaacacaatg cgactatttt atttggtaga actgtgagga tagaaaagca gaagtattgc
     4801 ttgaattttt gttttacttt ttgattttaa atttagttga ctaacggttg ctgttctggt
     4861 aagctagtac tgttgtaatt gtggactcca cagctacaac cgctcagaac aagtaataag
     4921 cacagcatat gctgtcactt cggatggcga gtctaagtat atcagaagga ggtgctttat
     4981 agcatgtacg caattattaa aactggtggt aaacaagtaa aagttgaagt gggacaagca
     5041 atctatgttg aaaaacttga cgttgaagca ggagaaaaag ttgtttttga cgaagttatc
     5101 ttagtaggcg gagaatctac aaaagtaggc gctccaactg ttgcaggtgc aactgttgaa
     5161 ggtactgttg aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata caaacctaaa
     5221 aaacattcac accgtaaaca aggtcatcgt caaccatata caaaagtggt tatcgacgcg
     5281 atcaacgctt aatagcgttt gagaaagaag gcctatatat gatcaaagga acatttaagc
     5341 gaaatgacgc aggtcagatc gtttcattca ctttgaccgg gcatgctgat gctggtcctt
     5401 atggaagtga tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact gtcaacggga
     5461 ttgcctcatt agccggtttt gagccgattg ttgaaatgaa tgaagaagaa ggcggttatc
     5521 tttataccga agtcacttca ggaatgacac aagaacaaaa caatattgct gagattctat
     5581 tagaaaatct tttattagga ttacaatcga tagaagctga aaactcagaa tatatccaaa
     5641 ttaaaacaat taatgataaa taggaggtgc agaccatgtt gttatctatg aatctacaat
     5701 tatttgccca caaaaaaggt ggcggttcta catctaacgg ccgtgactca gaatcaaaac
     5761 gtctaggtgc taaacgtgct gatggacaaa cagttacagg tggatcaatt ctttaccgtc
     5821 aacgcggtac aaaaatctat ccaggtgtaa acgtgggtat cggtggagac gacactttat
     5881 ttgcaaaagt tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa aaacaagtgt
     5941 ctgtttatcc agtagctcaa gaagcttaat aaataaactg ctctatctct tgtgtggcaa
     6001 gggatggagc ttttttgtaa attatgggat tttttttgac catacttttg accatacttt
     6061 cttagaagtt aatataattt gttagttttt gtgttgtttc ttcttttttg ctgttagtaa
     6121 tgtgtgtata tgtgtccata gtagttttta tatttgcgtg gcccattctt tcttggactt
     6181 ctttgtgatc tgcgcctgcc tcatataaca ttgatgcatg tgtatgtcta aaaccatgca
     6241 gaccgatata aggtaagccg gcacgctcac gatatacatt gtatctttct gctactgcct
     6301 gattaattgt gaaattattt tcgtcattgg taaacaaata attagcttta gtaaatccta
     6361 attgaaataa gtactttctt tgttcgagct tccaaaattt tagttgcttt atagtttttt
     6421 catctaaata aattattcga tttgattttt tggttttagg agtttcagat atataatatt
     6481 tatctgaacg agctacagtt tttttaatgg ccatttcatt tgttttaaaa ttaatattgt
     6541 cccaagtgag tgctaatatt tcgccgattc tacatccgct gaatgctaat agtctaaaga
     6601 gcgtatagtc tctgttttta aaatatggat tctgttcttg agatacttca tttagaaata
     6661 attccaactg ttcttttgta tataatttta atttcttttc tgttttaatt tgtggctttg
     6721 gaataagtaa atttaatgtt ggattatcag atgttaaacc aatatttata gcatacttaa
     6781 aaactttgtt catatagttt atgaaaagag ggtactgctt tgaagttcct ttttcactcc
     6841 aatcatttac taccttttga caatatgccg tattaatttt agaaagctta atatttccaa
     6901 atttgggata tatatgttta ttaaagatta ttttagtcct tgaatatgaa ctttctttta
     6961 cagttttttt gtagctttct agccataatt ctgctgcttc tttgaaagta gtatttgtac
     7021 ttgtgggcat tcctgttttt tgtaattcta gttctaacct agaaagtgct gtttttgctt
     7081 ctttttgtgt tctaaatcct cgtttagtag tatatttttt ctttccagtt agcggatcaa
     7141 taccgagata agttttaaag taccatgctt tttcgccatt tttcttttta tattgtttta
     7201 tcattgccat caatattacc cctattcaaa ttctaattct gccatgctat cattgaaaaa
     7261 ttcgatcagt ccttgttcct ctataaattc aagccatatt tccttcccgt taattaatgg
     7321 aactcctttt tctttacgaa ttgtttgata caagttagtc acgacttgtt tccacatttc
     7381 taatacagct ttatctattt tcttatcatc ccagcgtttt ttttggggtc tatttttgtt
     7441 tttttcaatt tgataattag tagctttgat gataaatgtt tcgtatccgg ttgtgctttt
     7501 ttctaaccat tctttgaatt ctgtatatat catttttgtt cacctttcta tttttgaacg
     7561 tatgttcttt tttgcttaaa aaattttaga gagctgcata acaatagatg cgagctttta
     7621 ataattcttt cacaagtggt tcgaagcgaa cgcttaaatt attatcttct ataaatttca
     7681 tgtaattgat attgtgtggt tctaattcag actttgcaat atatctatct aataattttt
     7741 caatcatgaa gcgatcagcc tcatattcca tttttgaatg gagagagaat gctaagttat
     7801 ataaaaaata attatcatga tgtttggaag cgtgtcctaa ttcatgtaat aaggttcttt
     7861 gtcttctata cttagataaa ttagctttta ttactatggt attcatacaa gcaacataat
     7921 ggccatcggc atcaaggttt tcacgctctt ctaccttaac gccgagttca ttaattatca
     7981 tttcaatttg actgtccaaa tgactcacct acttatttag ttatcttttt tctctaaata
     8041 tgcttcaatc atagctgata gtatttccct atcattatcg gtgagcggtt ttccgtcgct
     8101 actcataaca gatgctaaag cttcttccac ggttagtttt ctttcctctg gtgcaactcc
     8161 cgcgttagga tcatctgtgc gtcctagcaa ataatctgtt gaaacatgga aatagtcagc
     8221 aactgcattt agtttatcaa tggatggctt actctttttc cacgcataaa gtgaattttt
     8281 actaaaatta agtttttctt ccaattcgac aatagatatt ttgcgtttat ctgctaacat
     8341 ttttactctc tcaaatgttg tcataataat gattctccga tcatgcctaa aacacatctt
     8401 aaaaactttg tagataatcg ttgacatcta caaaacttta tattaatata tgttcgtaag
     8461 ctaatttaat aagctaacaa gcaataagaa agacacctaa taaagtaaac aaattcaagg
     8521 tcgggaaact taggatgtta atttataggg taattctgtg cttatttaac tatgcactta
     8581 ttctataaaa ctttttagaa actgtcaaca aaattagcta attttattag cttacatatt
     8641 taaagaaaag gaggaaacat catgaaacaa aataaaaaaa ctccttccgc aaaaggagct
     8701 ttaataacta tacattttaa agaccaaaag tttagtgctg ttgttgatga aattcatcca
     8761 caagagcatc aaaaaaattc ttttgcaact gacgagaaaa agactcagaa acgctctgag
     8821 aaagttcttc gaataattca atttctgttg gaaaagattc gtcattttgg aaatctattt
     8881 tgaattcttt attaacagtc ttgaatacgg tttgtttgaa taaagcgtcg acatccatat
     8941 taaattcttc catactattt tcacctcgct ttctattcaa aattatacca aagaaaggaa
     9001 tgagcaaaat gaacacacca caaattttta atttcggaca acaggaagta agaacagttt
     9061 tattaaatga tgaaccgtat tttgtaggta aagatgtagc tgagattctt ggatacgaac
     9121 gtgcagacaa cgctgtcaga aatcatgttg acgaagagga taggctaatg caccgaatta
     9181 gtgcatcagg tcaaaatcgt aatatgacaa tcatcaacga gtcaggcctt tacagtttaa
     9241 tcttaaaatc aaaacttcct tctgccaaaa aattcaaacg atgggtaaca agtgaagtgt
     9301 tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa gaagccttgc
     9361 ttaatccaga tacaatcatt caattagcaa ctaaattgaa agaagaacgt actggaagac
     9421 taatcgcaga acaaaagatt gcagaatacg aacccaaaat ctcatattta gatagcatat
     9481 tatcttctac agattcagta acaattagtc agattgcagc agattatggg atgtctcccc
     9541 aacagatgaa taaattactt cataaactag gtattcagaa aaaagtaggc aaccaatggt
     9601 tattacgcaa taaacatatg agacaaggat atacaaaatc tcatacaact gagattccga
     9661 aatctgatgg tggaacaaaa gttgtgatga atacaaaatg gactcaaaaa ggacgtttgt
     9721 tcatttatga gtcgctaaaa aaagaaggat atattccaga aattgatcta ttagaggaga
     9781 gatagttatg tcgcaggact tcattttaaa agtgcgtgtg gcgctagcta cgcatgacaa
     9841 gagtcaagct tggttagctg aaaagcttgg tatttcaaca gcatacatgt cggatattat
     9901 gaatggacgt agaaagccag ataaacaaat caagccaatt gaggcagtgc tagcagaatt
     9961 agaaaaggag aaaaattatg caaataacaa ttccagataa tttagtagtt tccgaattaa
    10021 ctacacagat cacgaatgca gtgcttaatt ctttggaaga acgattacac tttatgaata
    10081 aatcagtgga gcttcctcca tatccaaaca aatcagaggt aaaaaaagtt ttaggcattg
    10141 gtgatgacaa attaacacat tggataaact taggcttaaa aacacagcag tggagtaagt
    10201 tagacatcag aattgaacga tcagaactcc aaagattttt gaaagaaaac tttgagttct
    10261 aaaggcaaag gagaatgatt ttatgtccta cacattgcaa caagaacatc aaattctccg
    10321 tttgattaaa caacgcagga aacaattaca agatgatcgt gaagcgctta gaaaagccga
    10381 tgagctatca gatagacaag ctgaactaat cgcttctgaa cttgaggatt tgagaatgct
    10441 agaaataaaa aatagggaga ttagattatg aagaagacag acacactttt tataggattc
    10501 atcctagggc tattagtgat agtagcacac caaagtatta tcgggggaag tttgttcgca
    10561 gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa ggagcaacta tggcacgaga
    10621 agaagcgcta aaaatcggta aagtgattgc tgataattgg tggaccaata accgtcctat
    10681 tattttaagc aagcagcata tcgaaaagca aaaagcatgg caacaaataa aaaagtgact
    10741 ccgccggcaa gcatagagtc acaaagaaaa tacatctaag gagatgttac cacatggaaa
    10801 aagaactttc cactctagat caatatttga ctgatcctag ttggggcaaa tcgaatatca
    10861 aggaaacaag caatcgaaaa atcagacgta atcttttgac ggatgaagaa ctagcatgtg
    10921 atcaagacga tttgggaaat tttgtgagta tttgggatca tgtctatctt atccatttat
    10981 cgaggaagtc caaaaaacct gaatatatct atgtcatcga agatggcttg attgatgcgc
    11041 tagaagagta tgacagagat aacttgatcg atatctctta ttacggacca ggtaagaaat
    11101 acattgctga aatggaggca gaatttgatg agtgaaatca aagggataac gaactttgaa
    11161 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
    11221 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
    11281 aatgagtata aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
    11341 tacaaacctt ttcttatgga cgaaacaggg acttatgtat ctgtctcagt aacggttaaa
    11401 ggacacacgg aaaccgagtt atttcctgtt ttagattatc gaaaccaacc agttgttaaa
    11461 ccaaatgcta tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc attggctcta
    11521 cacggtctgg gattatatgt atttcaaggg gaagatattc caacaccacc tagaatcgat
    11581 acaaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca gatgggtaaa
    11641 gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt agggctctta
    11701 gctgataacg ttgaaactat tgaacagtta agctatgagc aatgcgcctt gatggagcga
    11761 gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt aaaccattaa
    11821 tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataggc gcaacgatca
    11881 atgaagaagt gaacattgat cgactaaaga cgatgtatga cggctacgat ggcgatcgag
    11941 tcattgaaat tcgttttatt gatcctagac gtttcactgt acagcaacga aacttcatct
    12001 atgcgctcat aggcgatatt ttcatcgata caggaatgcc aacggacttc tggaaggaat
    12061 tcttctactt ccgttttgaa ggtgtcacag gacgttctat aagccttaaa gacgagtcaa
    12121 gctcaacagt gagcgatgca aatgtattgg caaatatcat cttagatttt atttttgaac
    12181 accatatccc ttttaaagaa ggttatgaga ttttaccagc gaatcaagaa tattacttct
    12241 acaaatgcat cacaaaaaga gtttgttgca tctgtggcaa aacaggagct gatatcgatc
    12301 actttgacaa agcgctagga agacgaaagc gcaaagaagt tgatcattca gagtacacat
    12361 ttgcagcact ctgcagaatc catcacacag agaagcacaa gataggtgtg atcaatttca
    12421 aaaataaata tcaaatcaaa ggaatcaagt taaaccagga aacaattaaa aagttaagga
    12481 taggaggata aatttggctg agataagttg gatcaaactt aaaactacta tgtttgacga
    12541 tgaaaaaata cgattaatcc aagctgttcc tgagtcggat gccatcatcg ttatatggat
    12601 tcgattacta gtcttagcag gaaagactaa cgacgatggt ctgatataca tccagaggaa
    12661 catgccttat accgaagaaa tgcttgctac attgttcggc aaaaacgtaa atacagttcg
    12721 cctagcgcta actacattgg caaatttcaa catgattgat ctaggcagtg atggactaat
    12781 tgccatcagt aattgggaaa aacatcaaaa catcgaaggg atggataaag taaggctaaa
    12841 aaatgctgaa agaaaccgta aatacagaga aaggaagaga caggaacgtc tcaaattgga
    12901 aaatgacgtt agcgtgacgt cacgtgacgg tacagataaa gatatagaag aagataaaga
    12961 tatagataaa gaagaaaaga aaggtaagta ttctaacgaa cacttacgcc ttgctaaaaa
    13021 gttgcaaagt aatttaactg aagattttcc aaaagaaatg aataaagtag atatcgaaaa
    13081 atgggcagac acaatcaggt tgatggaaga aagagataaa gcatctatag aagcgattga
    13141 gtatgtgatc aattggctac ctacaaatga attttggttt ggaaatatta gaagtgctaa
    13201 gaaattgaga gaaaaatttg agaagctcaa attcgaaatc aaagcagaca agaataatca
    13261 taaaaagcaa agtcaaaaac tacagtacag caatcctagt gaatatgacg acttgccaat
    13321 ttaaaaagga gatgcatcac atggaaagcc tagcaaatgc tatggagaaa ctaataagaa
    13381 gagtattagt gcaaagtgga aaatgtccag aatgtagcga acctttgtat agttggcgag
    13441 ctaaaaataa ggatggttca gaacgttgta aaccaacatg catgagttgt ggttataaag
    13501 cgttacgtgt gaaagaggat ttacagaccg aacggatata taacgacagc ttaaaagcac
    13561 gagcattgag tttttttcaa aatggttcgg tattaacaga taaaactttg tttaaatgca
    13621 aaatggagaa ttatcacgta gtggaccaag aaacgaaaat tgctttagaa agagctaaaa
    13681 gctttgtaaa tgatgtctta ctgaaccatc ctgcacattt cattctatca gggaaatcag
    13741 gaagcggaaa aagccacttg tcaatggcga cagcttggga aatacttgag cgctcaaatt
    13801 atgacaagaa aatacttttt ataagctatc aagagttatt agagcaaata aagttttctt
    13861 ataacaatgc tgaactgaga aaagaaattg aaggatcact tatagccgat attaaaacaa
    13921 ctgatttggt ggtttttgac gatattggag ctgaattagg tagcggggta tcaaatagta
    13981 ggcagtttac aaacaacacg ttaaacacgc tcttggaagc cagacagaac aaggcaacga
    14041 tcatcacaac aaacttatct ggtcctgaac taagagaagc ctacggcgaa agaattgttt
    14101 ctaggatatt taagaattca gaaggttatg cgctgaaatt ccaacaaaca gcagacaagc
    14161 gcataaaacc agtgaaaggt agtatcgcat gaataaatac cgtaataaaa aaactgttca
    14221 tcgaggtatc aagtttgatt ctatcgcgga agcagagtat tacgatctag ccttgtggca
    14281 agctgaagcg aatggctgga aagtaaaact tcaggaaaga tttgagctga tgccgaaatt
    14341 tgaactagac ggaaagaagt atcgcaagat cgagtatatt cccgacttca cattttataa
    14401 aaacggcaaa cttgtcaaag tcgtagatgt caaaggaatg cagacaaaag actttaagat
    14461 caaggcaaag ttgttctgtc atcaatatca agtgccgttg atattagcta aaaaatatcg
    14521 gaatacgttc aaggaagagc gtttttaacg aggtggtcca tcatgacaac agaagaagtg
    14581 attcaaatgc gtattcgaaa cattcagcgt gaaattgacg atctggaacg aacaaaggca
    14641 gtgatggtca atgaaacggc gagaaaggca atcgatttgc acatagagaa tttaagaagg
    14701 gaaattcgta gattggagga atgagtgtgg ataataaggc tgcaatgaaa cgaatcatcg
    14761 aactgacaca ttctgagaat tggcaagaag acaaagaaat agttgcagaa gtccaaaagc
    14821 tcggcaaatc aatgtggact gaaaagccta aacggaaaac gccgagaaaa attgcaatct
    14881 ggcatggtga tcgaattcta gtaacgggta cagctgaaca gttatctgaa attactggac
    14941 tgagcaaaaa cattatttgg gatagagcta ggagcttatg gattgattca aaaggacgac
    15001 agtttaggta tgtggaggag aaaaaatgct agatatgaaa atcgaagatt atcgaattac
    15061 cagtgattct agaaacattg tcttatcgaa ggtaagacga gatgaggaag gaaacatccg
    15121 ctacaccgaa gcaaaagaag aatcacgagc agatatcgga tactttcaaa cggtctcatc
    15181 gtgtttaaag gcgatacaac gcgattacgt gttaagtgaa gaaagaacga taaaaagtat
    15241 tatcgagtac aaaaaagcgt tagaaaacat cactagacag tttgaacagg catgtgagat
    15301 cgaggaggag aaataatgga attcgtagac ttaaatgtac gtgggatcaa atgcgataat
    15361 ccagaatgtg attattctga tatggcagta aaatacgaaa attatccgca atggttgaac
    15421 aagccatgtc cgaaatgcgg cgctaatctt ctgacgcaag aagacttgga tgcaacagag
    15481 cagcttatgg agattgtcaa tcttacaaac gagatcctca aagattcagg attggagaag
    15541 caggatatga ataaatatat cgtacctgta gaagcgaatg gaaccggtga attgtcattt
    15601 ggagaaataa aaaagctgga ggaagaaaaa tgaacgaaaa taaattaatc aaactaggtg
    15661 tggcaggagt agtaatagta ggtattggag ttatcggagg atttaagttt ttcgaaaaaa
    15721 ttgataacgg atatgtgggt gtgcgctatt caatgaatgg cggtatcaaa gatgaagcac
    15781 tgacgcaagg tgtgaaattt gttgggattg acaaagtgat ccaatatcca attcgcttgc
    15841 aaactatcca atcaaaaaac atttcagtat caacaagcga tggcaaaaag acaacgattg
    15901 atatcaaata tgactacaaa gttgattcaa ctaaagcagc aaaaatgtac aaagaatttg
    15961 ggaatatcac ttcggaagat atcgaaagtg gatggttaaa atctaagctt caaaaagtag
    16021 cgcgtgaagt ttatgccaaa tatagcctgc ttgatgtcct ttcaggagat tcctctaaag
    16081 ttgaagctga ggtactaacg aactttgcta aatcagttga atctaaaggg tttgaagtcg
    16141 aagacgtaac acttggtgtt ccagatgtcg ataaagaaac acaaaaatca attgatgcga
    16201 tcattcgagc tggtcaagaa aatgaaaaag cgaagctaga tgcagaaact gcaaaaactc
    16261 aagctgatag tgaagcttac aagaaaacaa aagctgcaga agcggaagct gaatcaaacc
    16321 gtaaagttgc cgaatctgta acagataatt taattcgtta tgaagaagct caagctcgaa
    16381 aagagcatgg atgggtaaca gtaaacggag cagatactgt agttacggat gaaacaggta
    16441 aataatatgg gattctttat agctaaaatt ctcttgttct taggtttggt aggagtggca
    16501 tatctcgtgt atgccctcct ttccaaaact gatgacaaag aagatgacaa cgacgatgaa
    16561 atgaaatttt aggaggagaa gtaatggatc tcattacaca atacagtgac atcatcctca
    16621 agaaaatcat gatgaagatt cagaaagaca aaaaatcaaa agaacgagcg gaattagtta
    16681 agttggaaat ggctgaaaca ggatcaggag ttcgaacatc gaggcattgg aaagcagcag
    16741 caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg
    16801 atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt gtagaaaagt
    16861 atagagagat actgaatgag tatttcgagg aggactagat gtttaattta atgttagcgt
    16921 caattcactt tttattctat ataacgatat ttgtagctat attaattaat acgataaaaa
    16981 gcggtttttt aacaccaatc ggcacaattt taacattagc ttcatttgtt acaagttgta
    17041 ttatccaact taaatatata aaggagaatg aataatggac gaactaatca caaaagtaga
    17101 gcagtgggct aaagataagg gattggatca agctgattcc agcaagcaaa tgttaaaaac
    17161 gatcgaagag attggggaag ttgccgcttc tctagctaga aaagatgaat atggtttaag
    17221 agacggaatt ggagacgtag tagtaacctt gattatttta gctatgcaaa atgatatgga
    17281 tttgtacgag tgtctgaacc aagcatacaa cgaaatcaaa ggacgcacag ggaaaatggt
    17341 agatggtgta ttcgtgaagt cgagtgattt aaaacaacta gacgaaccga agaagctagt
    17401 tattccacaa ctcgtggccg gttggcttga gaaatttacg gaccctttta caaaagctga
    17461 aaaaatagcg tatttaatca aatccaaaga tggtgattca tattatttct gtgattggtt
    17521 tgtacgagat ggcatattga cgcaagaaca aggagaagaa ttactcgctt gggcaacaag
    17581 acaatcatat gaaacactat tgagcctata caacggctac gaggtcgaga aatctaagtg
    17641 ggtggtaaac gaaggcgatt tagtcatttg taaaggtaag catgaggcga aggtatattt
    17701 cgttgagagc gttgatgatg atggaatact tttggtgaac ggtattaaag atgaattttt
    17761 tactgatttg gacgatcgtt cggttgacga agaatcaatc aactactttt atgaaaattt
    17821 tagattgtta gcaaagaaag agaatttgga agccgagaaa gtggaggtgt gaaatgaata
    17881 tcacagaaga agctgaatat tttatagaat taccagcaaa acagaataaa ctttatgtga
    17941 attatgatat cgaagagaat atcagcattg atgcgatcag agtaactcgt tttacagaag
    18001 cggaaatcaa agcaattgat gaaagatact ggccatttgc tgtgccagtc gagaaagtgt
    18061 aggttcaatc ggatgatcaa aaaaataaaa actttgatcg atggttttct gctagaaaga
    18121 aaactcgtta aggttagaga attaataaag attcatatag atagcggtga acgttcaatg
    18181 tattgggtcg ccactgatag cgaaaagcag aatgttatga acatgatcag attttttgag
    18241 attgcattcg aagatggata ttttgcgact ggtgagtatt ttgatgcttc ttcatggatg
    18301 tcttcgaatc cagaagaagt gtggcaaatt tatttggaaa tgaaagagga ggcagaagga
    18361 tgagtgaaga tgaatacttt gaacagtagt ctattaacga cagagatttc caactggaaa
    18421 ggggtgtagt catggatgca tcggaagtag taaataaaat taatagtctc aaagaggttt
    18481 tcggagacgt cgaagtagtg ataaacgcac aaggctatga caacggattt tttaagcaga
    18541 ttaccgacat tagcattcag caaggaatgg aagacgaaga tggaaacttt atcgatgaag
    18601 tagccatttt agtgacttgc gagtaagtaa ttgacagcta aagaggagga agcggaatga
    18661 acaaaaaaga attaactgtt gagaaagagc caaaatatat agtcaaaatt ggcaaacttt
    18721 acttaaagga acctttaggt gatactagca attttacaat tctcacaact tgggataaga
    18781 aatgtgctta cccgtttagt agtttccata tggctgatat acatgctgct aaatttgagg
    18841 gaacagtgga agaggtggca gaaggatgaa aaaattgcac caactcatat ctgagaaaga
    18901 aagcgaactc caaaatttag aagatagttt gggattgggg tttccaattg ttgagcaagc
    18961 taaaatgact caaatcagtc atctacgact tgaatcggaa gatttaagac aaatagaaaa
    19021 atcaattcaa ctcaacgaca accagcaaat cgtgttggag tggttgaaaa gcgagacaat
    19081 tttaactaga gaagcaccaa tattatctgt taatgctttt tctgataaaa atttactagg
    19141 aaaattacct gataaagtac gcaaagctta taaactattg gcttgtaaac aagaatatga
    19201 agtcctagca gcattcgctc aatggggatt agaacaggag gaagcggaat gaattataaa
    19261 gtcggttggt ggaagaaagg ggaacaatat agatattgtt taggtatggg gcttgatgga
    19321 ctcgtttatt atcgaacaaa acgtcaggtc ttaggaaata gtaggtgtat cactggtaga
    19381 catccagact ttgataagtg gtttgaaaaa gctgaatatg ttggtttgga attggaggat
    19441 acagaatgaa gatttatgta gtaaagtttg gaaataaatt ttacagaagt gatgaacgtt
    19501 ctataggggc taacacatta tccattgtag acatactcca aagtgcaaga tggtttgata
    19561 atctcgaaga agctaaccaa gtttcacgac gacttggtgg attaacgcaa gcatatgaac
    19621 tggtcactgt cgatcatgag gaggtagaaa gatgaaacta aaagacggat tttacgctag
    19681 tagccacggt atcggcggtt taatgctaga catgccgaca aagaatccta aaacacgtga
    19741 gaaaccaaaa ttcaaagtcg gtgacatggt tcgctgcgaa gcagaggagt tcatctatcc
    19801 atttcgtgga tatgtagaac acgtctataa tcactccgcg atcattcgta ttgaaaacac
    19861 gatggaatgt gacaagttat tagcgaaaag caaagagaat ttagctgtag cgagattggt
    19921 ggatatggag gttataaaca atggaattta aaatctttga agaggacaca cgctatgaat
    19981 tagaagaaaa attaaatgaa tttgcaaaga ataatgaaat tcagcatata tctttaacgg
    20041 cttctaagtc cggttgtaca acttactatg cagctgttgt gagctacgta agtcaataaa
    20101 aggaactcga caaataaaaa agccggatcg ctccgactga ttcaataaat ccaacacatt
    20161 tattatatca cataaaggag cggtttgact tgatgcaatt gttacgagag gtagatttca
    20221 aacagacaag atgtaatgcg agagatgtgc tgaagaactt tcggcgtttg gagcggatgg
    20281 caggtcgctc tttgatagat attaagtcgc cgattattac ggatatgccg aaggcaccga
    20341 agcacggcaa taaggcagag gacgcgatca ttcagatgat ggatatagaa gcagagagag
    20401 acgcgattct agcggctttg atggctctta gtctgattag tcgtcagata ctctactaca
    20461 gcttctgtga cgtaaataag cactctaatt atgaaatagg gcaattgata cgaggatacg
    20521 gagagaagaa tgtagagaag ctgaaatcca tcgcattgat agaatttgca gaagcataca
    20581 aaaaaggcgt gttagttcag tatcgttgat tttgtagggt ttttgtaggg atagtgtagg
    20641 gtttttgagt gttttaacgt gatattatgg tagtgtcgaa agattagtga taggtctgag
    20701 acaaaataat aataaaagaa acatcgtttt attattgttt cacaattaag cttcgataga
    20761 cagcaacgga aatattaaga ataaggatgt gaattccaac tccttctaaa ttgttcttat
    20821 tatctatcat ccgttgctgt ttattaattt atgtattgga gggaaaacga atggaagtcg
    20881 agcaaataaa gtatcaaggt atggatcggg aacggctgat tgatattatt gagcaacaac
    20941 gtttagaatt gatccaaaaa gaagctgtat gtaaagagta caaaaaacat ttggaacaag
    21001 taattgagta tcattcagta gaaaagtaca gatcagtggt gcaaaaaaat agagtaacga
    21061 gtcctgatcc tcgtcaagat gtatcgatta gtatcaagac gccagtattt aattctgtta
    21121 aatcagaaaa gctagaaagt atctcaactg ctttggtaga aaaagcagct gaaatgaact
    21181 ccctagcaaa tgaagttagt cgtcttattt agtcacatcg ataacttgag agtctttggt
    21241 gattccgttt tttatagaat taatggtttg ttcagcggaa tatttagtca agtatgtttc
    21301 gctagttgct actacttcat tgttatcgga cttaataacg aagtaatatt ggccattagt
    21361 tgcttctctt ataacaaagt acaaattttt caccaccttt taatttattt cagcggacca
    21421 ctcgctgata actaaaatta tacgcttagt atttattttc acaatattaa tttatcgtaa
    21481 taatttttga gtaaatagaa caaaaaacct gcactagttt ccgctagtac aggcagtgac
    21541 tatatcggtt gataatctag catatattaa aaataattgc aagaaaagaa atttatagtg
    21601 gtcgatacca aattatcaaa gcaatgttat tttgttgctg tctattaatt tatgtattgg
    21661 aggaaaaacg aatggataaa gaaatcaaag taactgtcaa attagatttg actgaactaa
    21721 aagaactgct caacaaggct agtgaccaag tcgaacagtt acaagaaact ttagatgaaa
    21781 ttgctaattt taaaatccaa gtttcttagc agtatatttt ttagcagctg tactcaagaa
    21841 atcataccaa gtatcaaaaa aatataactt aactaaataa ttaaaaaagg tgggcaaaga
    21901 gaaatgatgg cgttaataat ttcaattttt gcgctctgtc ttaatgtcta tatgattgga
    21961 tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata gtgtcactgt
    22021 ggcggaagta gaagacgcaa aggtagaggt aggaagcgta tggcgcacta tcgggacctt
    22081 gtacaaaact agaaaggact agtacgtgag tggtgcgatt ccactccagt gactttagca
    22141 actgagggtt ggaaatgggc gctcaaagta cacgagcaag gcgagtcgat agtaatcgat
    22201 ggaatcggtg taggttgctt gatagagcta taactgcacc tcattgttga gatgtagttt
    22261 ttgcatatta gatcactcat cgagtggtct ttttattttg cgtaaaggag gctgcataat
    22321 gagaaactac tggtatatat cactaacaaa taagtaccca caaccaaacg cagatgatcc
    22381 aataagagtg gttcgatcag tccaaatcaa aaagaaatac tccatcattg aaatgaccag
    22441 agaagctaca ccaaaagagg ttgataagta caatcttcgt tactgtggcc atggatattt
    22501 tagtgagcag aacatacaga caaatataaa aaaatatcat taacatataa caaaggtggt
    22561 gatggaaaat gagtaagttg aatcctaagc aacaagcctt tgctgatgag tacatcatca
    22621 caggcaatgc ttatcagtca gcactgaaag ctggctataa agaaaactac gctaagaacg
    22681 cacaagaaaa attggtggaa aaaggtggaa aagtatccga ctacattcaa gagaagctaa
    22741 aagaagttca aactaagagg catttaacaa tggaagaagc tttggctatt actgcttcta
    22801 ttgcaaaagg agaaccacaa cgctttgaag ttgtcaagag agatccttat acaaacgaaa
    22861 tcatagaacg tgaagtgagt gaatattcag caggtttcaa agaacgtaac caagcacttg
    22921 agcattatta taaaataaac gcagcatttg tagataagca gaaagttgaa atttctgaaa
    22981 tacctacttt cattgatgat ataagtagtg atgatgatgg ctaaaaaact atctgaattt
    23041 cttccgccga agtttcattc agtatggaga gcgactttaa atcaagacat tcttaatata
    23101 gtttgtaaag gtggccgggg ttcaggaaaa tcatcagata tagcgcatat cgttactcag
    23161 ttacttatga gatatgcagt gaatgctgta ggtatacgtt atgttgataa tacacttgag
    23221 caatctattt acgaacaaat gaaatgggca attgagaagc agggagtatc gcgcctattt
    23281 aagtttaata agtcaccact taaaattacc tatcttccaa gaggaaatta tatgatattt
    23341 cgcggtgctc aaaacccaga acgaatcaag tctttaaaag atagcaagtt cccatttgct
    23401 ataggttgga ttgaagaatt agcagaattt aaaacagaag atgaagtcac gactatcacg
    23461 aactcccttt tacgtggaga attagatgat ggtctttttt ataagttttt ttacagctac
    23521 aatccaccta agagaaaaca atcttgggta aataaaaaat atgagacttc ttttcaaccg
    23581 gacaatactt ttattcatca ctcgacctat cgagataatc cattcatctc taaggaattt
    23641 ctgaaagaag ttgaggcaac tagagaaagg aatccaagaa gggctgagtg ggaatatgat
    23701 ggaaaagctg tggggtcagg agttgtacct tttgataatc tacaagttaa gaaaggttct
    23761 attacagatg atatggttgc taactttgat aacatccgca acggtttgga ctatggatat
    23821 gcaacggatc ctttagcgtt cgtcagatgg cattatgaca aaaagaaaaa cggtatttat
    23881 gcaatcgatg aaatttacgg cgtgaagatc agcaatagag aatttgcaaa caaagctaaa
    23941 tctaaaggtt accaaaatga ggagatattt tcagatagcg cagagccaaa gagtaatgct
    24001 gaattagtta atgaacatgg catgaaagga ataaaaggcg tgaaaaaagg acctgattct
    24061 gttgagtacg gtgaacagtg gctagatgat ttggctttta tttgtattga tccactacgc
    24121 actccgaata ttgctaagga attcgagaac atcgactatc aaacagatcg tgatggaaat
    24181 cctaagccaa ggttagagga taaagataac catacgattg atgcgacaag atacgccttc
    24241 aacgaagaca tgtgggccaa aaagaaatca accgttacta aagagcagcg gaacaaaatc
    24301 agaagaatgt tttaaggagt gtgagaaatg gataaggtaa acgaatttga atacggtgct
    24361 gatatacatt attcgaacga cgtgaacaca aattatgtaa agtttagcgt agattccaat
    24421 cttcactata ggtttagctc agcagaagat ttacttaacg atttagatac tttagcagca
    24481 atgataaaac atcatcatga atatcaggta aaaaggctaa gtgtattaga tgattattac
    24541 aaagctagaa atacaaatat catggataac cgtagacgta gagaaaagga aaaagcggat
    24601 catcgatcag cacataactt tggaaaagtt ctttgcacgt ttgatgttgg gtacaacaca
    24661 ggcaatccta taaaagtgca aatcgaggac acaaatcaac aaaaagaaat cgaagagttt
    24721 aatactaata atgacataga tgggttaaat gctgaactct ggcttgatat ggataagtat
    24781 gggagagcct atgagattat ctatcgagat tcagatgata cagattatgt tgatttggct
    24841 aatgtatttg aaacgtttgt tgtatatgat actacagtaa aacgagagcc tattttggct
    24901 gtacggtatc ctaagacaag attcaacaag gatgctgata aacagtacat tcaaccaatc
    24961 gtatacacaa aagaaaaaag tatcacttat gatgagacga cgctaacagc aattgagtta
    25021 aagaatcctc aagatgaacc gcatgaatat aaagaggtac ctattacgga gtattctcct
    25081 aatcgtttcc gaatgggctt gtatgaagat gtgctatcct tgattgatct atatgatgca
    25141 ggacagtctg ataccgccaa ctatatgact gatctaaacg atgctcttct agttattagt
    25201 ggtgatattg aagcagcagg actatccaca gaggacgcca tcaagcagaa agaagcgaat
    25261 atgcttttgc ttgagtctgg aactgatgtg aacggtaata aaacaagtgt gactgcagga
    25321 tatatttaca aacaatatga tgtgaacggt gtagaagcat acaaagacag agtgcgcaag
    25381 gatatccacg aaatctcaat ggttcctgat cttactgatg acaatttttc cggagtgcaa
    25441 tcaggagaag caatgaaata taaattattt ggatttgaac aaatgacggc aacaaagcaa
    25501 aggctattca aaaaaggcct tatgcggcgt tatcgtcttt tatttagtct aaaatcaagt
    25561 atttctgaaa tggataactc cgatttgaaa ggcttacgtg taatatttac gcctaatcta
    25621 cctaaagcca ttctggaaga gttgaaatct ttggttgatg ctggagctga actcagtcaa
    25681 gagacgatct taggactcgc ttcttttgtt ccagatgtac aggcagagtt gaaacgagta
    25741 aataaagaaa cgcaaaagca gactggtatt tttgattcag atggtgaaga agtaatttac
    25801 aacaaaaaag atgaaacagg ggagtgatta aatgaactcc caagaatatt ggatcaaacg
    25861 ggaaaaagaa tggcaaaagc aacaaattaa agatgataaa aagcgcatgg cagaaattaa
    25921 aagtcgcatg caatacgcac aagatgcgat acaaaaagaa atagacgcgc agtgggacag
    25981 tttctccaat ggtcagaaaa tcactcgtag cgaagcgatg aagcgtgcta gtgaaatgga
    26041 tgtcaaagca ttcgctcgca aagcaaagaa gtatgtcaaa gagaaagatt tttctcctac
    26101 agcaaaccaa gaattaaagc tatacaatct tacgatgcgt gtaaatagat tagagctctt
    26161 aaaagctaat atcgggcttg aattgatttc actgtttaat gaattggata agtacttttc
    26221 gaatgaatta acaaaagctg gtttagctga attgaagaga caagccggta ttttagaaat
    26281 gactattgct tcaagtggat atgcaaagct gatagaacta gtaataaaca gctccttttt
    26341 gagtgatgac gtgtctttta gtgatcgctt atggatgtat caatctgaat tgaaatcaga
    26401 attagatagg ttgttactga gaagtataac gatggggaaa aatcccaagc aacttgcatc
    26461 taaattggca gaatatttaa cagctgaagg acgagaaaac actaagttca acattcaacg
    26521 tttgatggtg actgaaacga ctagagttca ggtagggatc caagaacgaa gttacaggga
    26581 tgcaggcgtt acccagtaca tctatatagc agaaccaacg gcgtgcaagc tatgcatacc
    26641 gttaaataat caagcttttg atgttgccga tatgcagcca ggaagtaacg ctcctaacat
    26701 gcatccattt tgtcgatgca gtacagcgcc atatatagaa cgaatatcaa gtcgttaata
    26761 caaattaacg gctttttatt gtgccttctt acagcttaca ggcgttaaag agaaagctat
    26821 tttcggctga ctggcgtaac tggtcaaatt tatcgggtag cggcgtaacc gtggaggatt
    26881 aatcatggaa aaacgtttat ttatgccaat gaacttacaa tttttttctg aaccaggaga
    26941 tggtggatct ggtgatgggg gacaacaagg aaacccacca gctggctcac aagagacacc
    27001 gcccgcagta aaagaagaaa acaatactgg caaaacattt tctcgtgatg aagtagcgaa
    27061 aatgatcgct gctgagacga ataaagcaaa agcagcgtgg gaaaaagaac tagaagcaaa
    27121 aaaagaagaa gctaaaaagt tggcaaaaat gaatgcggaa gaaaaactac agcatgagtt
    27181 ggaacaaaaa gaagctgaaa tcgctgaatt aaagcgtgga caggcactat ctgaaatgac
    27241 gaaagaagct tctaaaatgc tgacagatgc aaatttacca cacgatgatg atttacttgg
    27301 tctgattgtt tctgatgatg cagatgccac aaaacaagct gtagcagtca tcactaactt
    27361 tgcttctttg attaagagag aaaacgcaag acaaacacca ccaaatgaag gtggacaatt
    27421 tacagcatcg aaaaatacta aagaaacagt ggctaaacta gccgctaaaa atcgaattat
    27481 caaataggag gaaaacttaa tgaaaaagaa acaactttta ccaatgaact tgcaaatgtt
    27541 tgctcaaaca tgggatccag ataatgtctt ggtatatgaa acgaaagagg gaaaaattcc
    27601 tgataaatat aatacgctca ttttgagtga agttatggaa aattctaaga tcatgcagtt
    27661 agcaaaatac gaagaaatga ctgacaaaga aaagaaattt gaatactttg cagaaggacc
    27721 aggcgcatac tgggtgggtg aaggtgaaaa aattaaaacg tctaaaccta aatggatgca
    27781 agctacgatg actgcaaaaa aactcggtgt cattcttccg gtttctcgtg aatatttaaa
    27841 ttataaatta tcagatttct ttgaggagat gcagccaaaa attgctgaag ctttctataa
    27901 aaaatttgat gcagctgcct tattaaataa agaaaatcca tttcctcagt cactagacga
    27961 atcagttatt agtgcgggga atgtggttga aggcggattg acttatgata atatcctagc
    28021 cttagaagac aagttagcag aaaatgaatt cgaacctaat gcgtttattt caaaccgaaa
    28081 aaatcgtaca gaattacgtt ctgcagctca aacagtcggg tcaaatgttg agtttattta
    28141 tgatcgctct gctaatacaa ttgacggatt accagtagta gaccttaagt ctttagataa
    28201 agggactctt tacgctggag acttcaacta catgttttat gggatcccat ataatatttc
    28261 atttaagatt tctgaagaag cccaattgtc tactttaact aatgaagatg gaaccccagt
    28321 taacttgttt gagcaagaac tgattgcttt gcgtgcaaca atggatgttg gatttatgat
    28381 tgtaaaagat gaagcatttg ggaagatttc cccaaaagcg taacgcctgc taccggtatt
    28441 gtgccaaatc aaaagacatg gaccggtaaa gtaggcgata ctaaaacatt tactatttca
    28501 gctgtgcctg cagatgctag cgatgcagct actgttgttg cagctactac agcaacttca
    28561 agtgatggag ctatcgcaac agtgaccaaa aatgaaaatg gtggttttga tggaacgatt
    28621 gcagcagaag ggtcagcaac attcacattt acttctggag aattcactac ttcaatcaat
    28681 gtgacaggtc aacctgctag ttaggaagta aaaatatgat gattgcagat gatattaaaa
    28741 aacttcttaa aggaacacta gatgaaaagc ttgaagttat tgagcgaaga acgaatgagc
    28801 gtatgaaaat cttgttaaat acgcaagaag ttcctaaaga atttgaaaca gttgtatatg
    28861 aagtatcgtt gaaaagattc aatagaattg gtcaagaagg tatgcagtca tattctcaag
    28921 aaggtttatc tatggctttt cctgattcgg atttttcaga gtatcaaaat gagattgacg
    28981 aatttaagcg taaagatcag gaagagttgt acaagccaaa gcgagggagg cttaaattta
    29041 tatgagattt acagatgaaa ttatatttgt taaacgttca tctgactcta aatatgatcc
    29101 agatctcggt gagtgggttg aaggaaaacc agaaagaaca agaacagagg caaacgtgac
    29161 agatattggc actgatagaa gtgtgactat ttttggtagt gtggaagaag gggcgaaggt
    29221 cattaggacg cagcctcttt tttctatccc tacatttgac tatatcgaga ttgaaggaaa
    29281 gacttggcaa caaaaaacag ctagaaatcc agcatataga aatagtttaa ttgtgcaaga
    29341 ggtggttctt gatgaaggca caacttgaat ataaaggaat cgatcagctg atgcgacatc
    29401 tgaaaaaagt agcaacgctt aatgacgttc aaaaagtcgt gaaaagtaat actgctgaaa
    29461 tgactgaacg aatgcaaaaa ggtgcgccag tggatacagg ttacttacga agatcaataa
    29521 acatgaatct tttagaagct ggtttaactg gtattgtagg accgacagca gactatgctc
    29581 cttatgtaga atatggaact cgctttatgt cggcccagcc ttatgttaga ccagctttta
    29641 attatcaaaa agtcaaattt atggctgaaa tgaaagcctt ggtgaaatga tgattaagac
    29701 aagagatcag tcggtttttg atgaactttt taaaatatcc caagaaaaac taggatacaa
    29761 aacatatgat tacaaacctt tagataatgt tggttatcct tttgtggaat ttgagaacac
    29821 tcaaacgatt cacgaagcga ataaaactga tattaaaggt actgtgattg tagttttatc
    29881 cgtctgggga ttacagaaaa aaagaaagca ggtgtccgac atggcatctg ctctttttaa
    29941 tgaagctaga ttaatagaag ccacagaagg ctattattgg gctttgaatt atcaagcaag
    30001 tggaattcaa gtgatggatg atacaacaac gaatacaccg ctaaaacggg cggttgtcac
    30061 acttgaattt agaattagat aggaggaaga acatggaagc attaaaaggt attgatgtca
    30121 ttttgcttta tcgcttattg aaaaaagaaa ctcaggaagc tgcttggaaa atggcatttc
    30181 aaacagaaca cgaaaatggt ttatcaagag attcagactc tacagtgaca aaagacggaa
    30241 acgttcaaag tttaagccca gttgaatatg atttttcggc tacttcaata gttgccaaag
    30301 gcgattctca tgtagatgaa atgaaacaag ccttattaaa tggcgatatc attgaaattt
    30361 gggaaatcaa caaagcagaa cagggcacag atgatgatgc aaataagtac aaagctactt
    30421 attaccaagc atatgtgtct gaatttactc catcggctgc tgcagaggat aacgttgaat
    30481 taagtttatc atttgcagta aatggtgttg gtcaagatgg ttatgcaacc ttgacagaag
    30541 accaagccga tgttgttcaa tatgcattca aagataccgt gaaagtaact tcgacaggag
    30601 cataagaggg cttagatgct ctctttttta ttttaggagg atgaaaaaca ttgaaattaa
    30661 aaattaaagg taaagaatat tcgtttaaat ttggcactaa atttgtacgt gaattagaca
    30721 aagtgatgcc tttcatcgat ggaaatatgg aattcggaat gggactctca gcaaaagtct
    30781 taccggaatt acgttcttat aatgtcaaca cgttgtcacg agtcttagaa atagcaaata
    30841 gaacagaaga agaaactatt acgttggatg aaatggatga ttacatcgat gaagttaaag
    30901 acatcgaaaa attgtttgat gaagtcctaa aagaattggc ggagtcgaac gcgggaaagt
    30961 tagcggtccg aaacctgaat cagaaattga aagaagcgga aaaacaacaa gcggaataga
    31021 ttctgcactg gcatacgaac aaattcttat caattctttt cgatatttgg gaatgaccaa
    31081 tatctcagat atcgaaagaa tgacgttata tgaatacaac attcgtatga ctgcagccca
    31141 gttatcttgg cttgacaaag aaaagttgat tcacgaatta gcgtgggcaa atcagcaagt
    31201 ccaagcggag aaaaaagtag gcaaaaagac agttcctgta tatcgatcct ttgaagaatt
    31261 cttcaattat caaaaaatcg aagattcaat catgggagtt tccgaacttt caaaacaaga
    31321 taaaaaattc caaagcttac taactaaagc taactcttga ggaaaggagg aaaatcatgg
    31381 aacaattttc tgttgaagcc ttattaaaag ccacagatag tggatttgta aagactttta
    31441 aagatgcaca agatgctgtt aaaacttttg aaaagaattc aaatagtatg acaaccgctg
    31501 ttggtaaagt gatgcaaggt actggtgccg caatgacaaa gtatattacc acacctctta
    31561 taggagtagg cgtagcagct gctaaagttg gtggtgactt tgaagcacaa atgagtcgtg
    31621 taaaagctat atcgggagca actggcgaca cattcgaaca gatgaaacag caagcgattg
    31681 atctaggagc aaaaactgct tttagcgcaa aagaatcagc tgctggaatg gaaaacttag
    31741 cttctgctgg atttagcgca caagaaatca tgaaagcaat gccgggtctt ttagacttag
    31801 cagctgtatc tggaggggat gtggctctag cttctgaaaa tactgctact gctttgagag
    31861 gatttggttt agaagcaagt gaagcaggac atgtcgctga tgtatttgct cgtgctgctg
    31921 cggataccaa tgctgaagtt ggagacatgg gagaggcatt gaagtatgtt gctcctgtag
    31981 ccaattcaat gggtatttct ttggaagaaa ctgcagcagc tattggtatt atgagtgacg
    32041 caggtattaa gggttctcaa gcaggtacaa cgttgcgagg agcattgtct aggttagcaa
    32101 ggccaacaaa ggctatgcaa gatacaatgg ataatttagg tgtttcgttt tatgatgctg
    32161 acggtaaaat gaaaccttta aaaactcaag tagaattact taaaaaagct tttgaaggcc
    32221 tgacgcctga acaacaacaa aatgctttag taacactata tgggcaagaa tcattatcag
    32281 ggatgatggc tttgattgat aaaggacctg attcattggg caaattaaca aaatctctga
    32341 aagattctga tggtgcagct gacgatatgg ctcggaccat gcaagataat atgaattctt
    32401 ccatcgagca aatgtttgga gcttttgagt cagcagctat tgtaattcaa aagattctag
    32461 caccatccat cagaaaagta gcagatgcca tatccggctt agtggaaaaa tttgtaagtg
    32521 ctccagaatc aactcaaaaa ttagtagtgg ccataggagc aatcgctatt gcaattgggc
    32581 cagtattgta tgcattagga atgctggtta aagcgtttca aaccatgaaa gtggggttag
    32641 gtgtattagg taacggaatc tctttgttca agaaattagg ttccgccata ggttttctta
    32701 ccagtccagt cggattggtt atagctgcgg tagcactact tgttgtaggt ttcatctatc
    32761 tttggaatac gagtgaagat tttagaaact tttggattgg cttatgggag ggaatcaagt
    32821 ctgctgtaag ctcggcagta gaatggattc agaatgcatg gaaatctaca ggagaatggt
    32881 ttaacaattt atggaagtcc attaaagaag gtgcagaaaa tgtttggact acaattcaag
    32941 aagctcctgg gaaagcggca gattggatca agaataaatg gactgaaaca aaagagttct
    33001 tttcgagtat atgggacggc atcaaagaag ctgccagttc cgcttgggaa ggaattgtaa
    33061 acattctagc accgtatgtt attgccataa aaaacgtttt tcagccaatg attgatttct
    33121 ttacgaacct atggtctcaa attggatcaa tcgcaggctc tgcatgggaa attataaaaa
    33181 ctgctgtaat gggtccaatt ctgcttttga ttgatttgat aacaggcaat tttaatcagt
    33241 taaaagaaga tgcttcgatg ctgtggacta cattaactac aaatatccaa aacattatca
    33301 cgacgtttgt agatatagtt gttggttatt acacagcctt aaaggatact gtgataaata
    33361 tctggaatgt gttaacttct accatcaaag atgtgtggaa ttcttttact acatggatca
    33421 aagagacaac taacaatatt gtaaatagta ttaaacagga atggaataac ctaaaacaag
    33481 ggacaatcga tctgtttaat aatatgattc aaggagcgaa agatttatgg aattctttca
    33541 aagcttggtt tattaatcta gttattggaa ctaaggataa catcattcag ggatgggaaa
    33601 acctaaaaca aggcactata gatactttca acaatttagt aagtggcgct caaaaagtgt
    33661 gggataattt agtaaatgct gttagtgata ctgttgatag agtagctggc tggtttgata
    33721 acttgaaaaa tatcgattta ctagcagccg gaaaagccat catggatagt ttcctagaag
    33781 gtttacaaaa tgcatggaaa tctgtccaag attttgttgg aggtattggt gactggatta
    33841 gggaacacaa aggacctatt cgatacgata gaaaattgtt aatcccagct gggcaagcaa
    33901 tcatgaatgg actaaacgca ggtttaacta atggctttgc cagtgtccaa agcaatgtag
    33961 gaaacatggc taatatgatt gcagatagtt ttactcgtac acctgatatt gatctttcag
    34021 cgaatttaaa aaatgcaaat agaaatttca caacacaaat agagcatagt gttaactacg
    34081 gcaaaaataa acgtcctgca gttttcaata ttcgcttagg aaatcaagtg tttgaagcgt
    34141 ttgttgagga tatttcaaac attcaaggta aagaggcgga tattaattta ttgttctaga
    34201 aagtgaggga aatatgatgg agtggcataa tccaatgtat gaattcagag acacggttaa
    34261 aaacgacagt cagaaaacat ggataccgac atctgcattg aattatgatg ggaagtttat
    34321 cgagaattat atagaagggt accagacttt gtatgtggaa ggtagagaaa tggtttcttt
    34381 agagattgag agtgaagcgg tgagcatagg tgttcgtatt agttctcaaa ggcttccaga
    34441 gaggatttta actatacatt ttaaattaga agaaaagaat cctattgaat ttcaacgaag
    34501 ctttaataaa ttgatgaggc tgctttacag ggataaagat gtagaaattc actttaacga
    34561 tgagttggat atgtattatt acggaaggta tcagacctgt gataatattc caggaaacgt
    34621 tcatagtgta atatctagtt tctctataat ctgttctgat cccagaaaat atacacgtat
    34681 atttgaaaca aacggcatag tctcagaata tcttccgtat gaagtagctc cgatttcaat
    34741 tagtttaaag gctaataatg atgggagctt aagaattaca aatggccgtc aaaatattag
    34801 tgtgactaac tcaatgataa aaaaaggtga ctttatcgag atggacatag ctgagggaaa
    34861 agtttttgta aatggagtga ataaaacgag aattcttgat cttaccagct catttaaaaa
    34921 ctttatggtt agaaccggtg atctagttaa gtgtgataat ggcactccct taatacgata
    34981 tagaggagtg tggctgtaat ggacaaagat gtttattttt tcgacgatag ccaaaagctt
    35041 ataaaaatag taggggaaga tagacttttt tcggtagttc aagaaaagga aattacacca
    35101 agtaaagatg agttaattaa tgataagtta gcagttagca tggagtttga taacgaaatt
    35161 aaagagtcag cttatatggc ggttcgtgaa agcgagtcgt ctttttctat gtataaaatt
    35221 atcggaattg ctgatccggg ttcattgttg atattcactg ggattaattt tgggcctgat
    35281 gaattggatg cttatatcat taatgatatt cgtccggcta acgagttttt tcagaaaacc
    35341 atccagagag tcatagattt tacatcaggt gagtggcgag ttggtcattt agattcaact
    35401 ctgccagcgg tttctatgac tttttactac tgtagtatcc gtgaagcctt aaaaaatcta
    35461 caaacgttag gatgcgagat tgtttttagg tgcaatctaa gtggagaagg aatcacagat
    35521 aaatggatag aagtttacaa gcagattggc gaatacagta atgagcgata tgagtacggt
    35581 gataaagcct tgacaattga aaaagaagta gatcgaagta acatctatac ttctctaatc
    35641 ggccgcggcc gtggtgaaga ggttggagat ggttatggtc gccgaattga gtttgatcaa
    35701 gtatactggt ccaaatcaaa aggggatccg ttaaacaagc ctactggcca gatatatttg
    35761 gaaatccctg agatgactga aaaatatggt attcctacta aaaacggaaa acgtcgtaag
    35821 cgtgagaagg taattatatt cgaagactgc gaagatcctg ttgagctaat tcagcttacc
    35881 tatcaagaat tggttaactg ttcacgtccg ctagttcaat ttaaagcaac tattttcgga
    35941 gcagatagtt taggtaatat tatacgtatt catcgtgatg accgcggcta tcattacgag
    36001 actagaattt tcagtgtgaa gattgatcga ttaacaggaa aagtcgaaac tggtttaggt
    36061 gataatttaa atacttcatc aacacgtcaa gcttcaaata ctcaaactgc catacagacc
    36121 cttgatgaga agaagatgac cttttatgag tccaccgaag tttctaaatg gcagtcggat
    36181 atcatccgcg gagcaaaggg tggatcaatt atcatgatga atccttggga tactggtaaa
    36241 ggcgaaagtc gtcaaccata tcagatggtt tggatgaatg gggatagtat tgatacttct
    36301 aaccattttc ttgtagctaa ttcggaaggg attggtttta ttgatgggaa attcaatgag
    36361 tcaaatttca aaacggcatg gacgattgat ggaaacttca atgccaatta tatccaatct
    36421 ggacgtatta gagcagatat ttttgaaact tcatttaacg ctgttggtga tcagctcaag
    36481 ttagtaaaag gagctttgca aattgtaaac agcaataaaa aaatcatgga attaaccaaa
    36541 aaagggatgg agttttggaa cgccaaagaa tccattggca caatcggtac gactgattct
    36601 gcaggcaatc cttttcctgg cgcttccact cctacaccgt tagaagataa ttcattagtc
    36661 attcgaacaa acggcgatgg aaaatatatt ctcatttcac caaaagaagg aaaaggttgg
    36721 gtaatacttg ggaatggaac ttctatatta tttggaagtt taaatctaca ggaaaagcta
    36781 aatgcctttg gtgatgcgga atttatgaaa aacgtcaata ttcgcggaaa actcacaatt
    36841 aacggacaag aagtattccc tgggcaaggc ggaagtggaa ataatgatgg cggtagttgg
    36901 aatggcatgt atccaccaga ggttaccagt caagcagaca aatttgcttg ggaattatgg
    36961 gtcatgcttc tttctagagg gtattccaaa gcatccattg ctggaatcct tggaaatgtt
    37021 caaggagaag ccggtgctgc aatgaatcca gatattgcac aagtcggcgg tccagcttat
    37081 ggaatcgttc aatgggatgg ttcggcatat cctttggttc ctccagcaac atgggatggc
    37141 aggacttatg ttcaaaacct gatgagagcc gcaggaatta cagaagatta tcgaacaatg
    37201 tcagctcaag ggaagttatt agattggacg atgtataacg gtcaatggtt aggaattgtt
    37261 caaccagcaa gtgtttctgg atttaaagca atgactgatc cagcagcggc tgcttatact
    37321 tttgaacgaa attatgaaag accagccaca actcatccag aacgacaagg atgggcagtt
    37381 aattggtata acaaattcaa agatcttcaa atcccatctg ctggcagtat tctcagtaca
    37441 gccaaaagtt tgatgggtta tttccattac tcgcaaccgt tacgttggaa ttttggtagc
    37501 gtcgagaatc ctgatcgtaa tggatatgcc gactgttctt cttttgtttg gttagctttg
    37561 acaaaagcag gatataaaac cgcaacacgt ggaactctgt ggtatacagg ctcaatggct
    37621 gcggatgcaa gagggccgcg tcagtatctt actgaaatat ctccaaatga agcgaaagcc
    37681 ggagatatca ttattgtcaa ccaaggagcc ggtgctggta atgatggaca caccgctatt
    37741 ttagcagagg attggaaggg atatagcacg tctattgttg aaatgggcgg tatgaattcc
    37801 aatggtgttg gtatcggtcg cgtcgattgg tctttcggtt atttgttaaa cggtggcgat
    37861 gtttgtctcg ccagagcgaa gaaatagagg tgatcttgtg atcgaagaaa aaggattaaa
    37921 tcatttgaaa agtttgttga atcaacctat cggaaatcat caatgttatg cattatctgc
    37981 ggaatatgcc ggtgtgatga ttggacctga catgggggcg gggactaaat atgagatcaa
    38041 agtacgtcat ggcaatgtat tttccgctgc tgaaattgga cgagcctatc catgggcatt
    38101 gtatttatgg acggttattg ttcatcccga gtatgaccaa ctagttgttg gctcaattat
    38161 caattgggaa agaaacgcaa aaatcagtga tacatttgaa agccatgaat attacggcca
    38221 cacaggtgta atcaaaggtc tagaaaatgg gcgtattcaa acctatgaac aaaatgcaga
    38281 atcaggtgga attgtggcgg aatatgatcg tgaattcttc ggatctggtc agatagcttc
    38341 tatttgtatc ccacctgatt ttgagaaagg agtggtaatt aatggcaaag tggaacgtag
    38401 tactcagcac aactgaacct tataactatg tcggtatgat tcaggtacgg caaggtaata
    38461 agaattcaga agttatggaa gcaaccatca ctgaaaatgg aatgccctat gatctaactg
    38521 gttgtaaagt ctattttgaa tcagttgtcg gagacaaata cccagttcaa ttaggtacta
    38581 aagtcattga tgcaaaaaaa ggaaaaattc agtacacatt tgatcaatat tcgatgcaat
    38641 gcctacatcg ccaaacagca gattttatca tttataaaga tgatgagtta attgctacga
    38701 cgcaagattt ctcttatttt gtgattaaag ctgtctcaaa aacagagggt gaaatgggat
    38761 cgtattggca gacagtcgaa gatctaattg cggatatgtc agcttttatc aatgaaaaca
    38821 aaggtgactt cacagattgg atgaatgcac gtaaaaagga atttgaacaa tggcgacaag
    38881 accaacaaaa tacatttgag gcatggagag aaggacaaga aaccgattat ttaaaatggt
    38941 ttgaatcaat caaagatatt ttgaagtcta tcgatccagg tggagtaatg ttagccgaat
    39001 taatggatgc acgtgttgat attcaaggag ttcgtcatgc gtctatttca gagcgcttgc
    39061 tggcagatat ggattatcta tatcagaaat tacgagcaac acttttcacg attgaatacg
    39121 gtgagattga agtgactgat attttgcagg atgatctctt ttcagataat cacgaagtcg
    39181 aaaaagttgg aactgtagaa ttcccgattg aagaaggagc cttgatcatc gcaaccgttg
    39241 atgatccaaa gcaaaatgtg ttcactcttg agaaagttgg ggtgatctaa tggctaaaac
    39301 taaacgaatg atggaaacgg atgaaaaaac aggtgtgcaa cgccaattct ttccaatcac
    39361 acatgcttcc gcagttcttg ggctagaaga aataatagca ggagaagcaa cagttttatc
    39421 tgttaatggc aaaattggag ccgtcgtcat tactaaagaa gatttgggat tagagaatgt
    39481 tctcacagaa ttaccctatg caagtgaaga agatgacggc attatcacgg cagaaatgta
    39541 tcaaaaaatt ttaaacagtg gagaaggtga ctacgtgttg ccagttgcca ctatcgaccg
    39601 tttgggtggc ataaaaattg gtgaattatt gacgattgat gagacaggaa aagtctctgc
    39661 agtcagacaa tctgatgtca atttttcgct ggagttaaaa gaaaaactcg attcactgaa
    39721 aaactatact gcaggagaaa acatcactat tgatgaagat ggaaaaataa atgcagaagt
    39781 gactggtttt tacgttttac ccactgcctc cgaatttgta aaaggtggta ttcgtgtcgg
    39841 tgaaggttta acgatgactg atgatgtttt atcggccgat aagcaattca actatactgc
    39901 cggagctaat atcagtattt caaacacagg agtaatctct gcaacaggtg atggagaagg
    39961 tggcggcgtc actcaagaat atgttgatca aaaaatgagt gaagcttatc aaaatgcaca
    40021 agcttataca gattccaaaa taccaaatgt atcgtttgaa aaagtagggg aggtatagac
    40081 aaatgacaga tattgtaaaa gtaaaacaga atgatgttca ggtttaccct caaacccatt
    40141 gggatgctgt tgagggtaaa cccgaaacta tcaagggaga taaaggagac cctggacagg
    40201 ctgccacaat tactgtaggg acagtaacga gtggtacaac agctagtgtc acaaatgctg
    40261 gtactgcaag tgcagcaaag tttaattttg tattgccaaa aggtgataaa ggggacaaag
    40321 gcgatcctgg agccaatgca acaacgacag ctgtggctac gacgacagca aatggtttga
    40381 tgtccaaaga agataaagtg aaactcgatg gattagccaa tattacattt gagaaagtgg
    40441 ggacggttta atggctgata ttgtgcaatt aaaagaagac ggagttgcta agtatttgaa
    40501 aactcatgcc aaaggaattg atggtgtgga gggtgtttta gtaaaagcca caggtaatga
    40561 aacggtctta ggaacgaaaa attttaaaga cggattgcaa ttcaatggat tgcctgtgca
    40621 agcaggtatg atcgagcgag ctatcacact agctgatcga agtgatacaa caaacgtaac
    40681 tgatgtgaat ggaaaaataa tcagaatcgg aaacatcgta tttcttactt ttaattttaa
    40741 atgtggcaca tggcccgaag gatcagaaac tcgttggatt ttaaaaatcc cggacggttt
    40801 caaacgtgat caaggctatc cagcgcaaac cgcgctttca ctagttagaa atgcaagtca
    40861 gccagcggat gcacgtgcat tcattgatca aagtagcatc atacaagcca aatcaggttc
    40921 aggtagttca tatatttcag gtatgtggat cactcaagat ccttggccgg cttaacagat
    40981 tactaattgg aggaagagta aaatgaaaac aatttataaa gtattgtatc caatgggata
    41041 tgaagaacat caagtagaag ataactttcc aacatcttta ccatttgtcg aagttccacc
    41101 tattttgttt gaaaagaagg aagatgaaac ggatgaaggt ttcggaagaa gacaacagtc
    41161 gcaatttttt aacttcactg aaaataagtg ggaagaagca gttacacaag attattctaa
    41221 aaaattagaa ctactagaaa atttatctat aggtttacag gtagataata ctgcattgaa
    41281 aaaatcaaat gaagagttaa cggaaaaagc agattcgatg gcgcagttga acgccaaact
    41341 gatgcttaat gatttaacaa ttaataaaaa aattgaagca atagaaaaac aaattggagg
    41401 tgcttcataa tgtttagttt tagcgatgtg aaaatgatgt atgattgggg ctgttttact
    41461 gacgatcaag ttcgaatatt cgttccacta tgcattacag acgaagaagc agataaaatc
    41521 attagcaaag aagagagcgc atcttaattg atgcgttttt ttgttggaaa gttggtggaa
    41581 catgaaagaa gaagcgctcc aagacgttgt ggagagatta gtaagaattg aaacaaaatt
    41641 agacaactac gaatcactta gagaaaaggc tgatagtgca aaagatttgg cagataaagc
    41701 ctattcagta gcactaaaca atgtagaaga catcaaggaa atgaaaaaca ataataaatg
    41761 ggcttggggc tatatgattg gcttaggcat tacaatcatt ggctatttcc tgactaaact
    41821 ataaaggaag cgaaaaaaat gattttaccc gataagtatt atcaagttat taaatggacg
    41881 gttttaacag tattaccagc tgcatctgct ttagtagcca tgttaggaaa agcgtatgga
    41941 tggaatggaa cagatatgac agtactcact atcaatgcag tagcaacatt tttaggcgtt
    42001 atcactggtg tgtcggctta taatttgaaa aaataggagg aaacaaatga aaaagaaaat
    42061 tactattact gtgatgagcc tgttaacggc tcttttttta ttgccaatta atggatttgc
    42121 ctatacgatc aacaatgaat tcaatttggg cgcaaacgaa ggtagttctc aagtagcgaa
    42181 taatcagtac attttactgc atgaaacggc taacgaaaca gcaacaggac gcaatgaagc
    42241 gcagtatatg caacgttcat ggactagtgc ttacactgct tacattgtgg gtgatggcgg
    42301 aattgtttac caagttggac aacctggtta tgtgcagtat ggggctggtt cgtatgctaa
    42361 tgccaacagt cctgtgcaga ttgagttaca acacacacat gataaagcaa tttttgaaaa
    42421 gaactataaa gcatatgttg aattggcaag agattcagca atgaaatatg gtattccatt
    42481 aacattggat actccttata accaactagg aatcaaatcg catttatggg taacgcaaaa
    42541 tatttggggc gatcatacag atccttacgg ttatctttct gaaatgggcg taagtaaaga
    42601 aaaattagcc tatgatttag ctcatggatt taccgatgaa aatccgacaa cttcagatga
    42661 taaaccagtc attgatccaa ctcgagcagg tgctgcaaat cctacactaa cagatggaac
    42721 aaattatgcc cacattgatc agttcggaga aatcgaaaac gcaaacttgc atgtagctgg
    42781 atggcacatc gctaactata aatacgagta tattttcatt atggactaca atactgggaa
    42841 agaattagct cgagtaagag ctgatggaat ttatagacca gatgtaaacc aagattataa
    42901 tacttctgga aacgttggct atcatgtatc tttcaatatg cgtaattttc ctaataagaa
    42961 agtctatgta atgatgcgtg caacgaatga tccagaaggg aacactaaag gcggagcaca
    43021 agattttcat gacaaacgtt ggtatttaaa tattccgcaa cgataaaaaa agcccctcgt
    43081 ttttgagggg aagtacataa cgatattgac tattatcagt tgtcttttta cgctttgtga
    43141 caacttgtat tggttatagt agataaattc taaagctatc gtaagagaat gtttttagtt
    43201 cctataaact ctaaacttag aattttagct cttttagatt gctggaagtc ttgggctata
    43261 atttttttct taacgtaaaa ggttaccggg atatatatag cgggggatat ttctcttaaa
    43321 ccaagggtca atattttatt tttataagga tgattaaatg cgatatctaa tttcatagaa
    43381 gaaccgtttc tatcagcttc agaaacataa ataacggatc cgttataatc ttctaggaat
    43441 atatctaaaa tgaaatcaaa cagagtaatt ctatctttga tagagccaaa cattttatgt
    43501 cgctgaattt tttcatatgt aattgaagaa gaagccagtt tactgcaaag agatttagga
    43561 ggttcattat atattttgtg tagtccaagc aaatgtggaa gttgaagaat atcaaatttt
    43621 atgtaaaagc aatctagagg ggtgaatggg gttgttaaaa ttgccgtttt gccatcaaga
    43681 tttgttagat attgattata agtttttttt aaatctttag gcatagaagg cgctcctctg
    43741 tttagttttt aacaaaaaaa aagagagtgc aaagtatgga taccgtccca tacagaccaa
    43801 cgctaggcta acccgcctgc gggggaacgt gagtgcactc ttctcacttt gattgatatg
    43861 attattatca atctagagct aactgaaaga tgaggtgata gatctatcct cgtaattaat
    43921 ataacattta cgtaaaattt atgcaagcgt aaaatataaa aaacaataag atgaaaaatt
    43981 aagaaaactt aatatatacg atttaatata actatttgta cgtgtggcac attattttga
    44041 tgcaatataa ttacaaatta taagtgataa aataacgttt ttttgaatat tgtaattctt
    44101 gctaataaaa aaccattgat ctcggaaatc aatggcaagg ttatagattc tagacactag
    44161 aattcaagac taagctaggg attgaacaga ttggatatcc gcgcaagtgc ttgtaggcca
    44221 tgttgacagc atggtctttt tttgcttaca atgtcattct atactacgta aaaaaagcgt
    44281 caataatttt tttactataa agctaagcaa atgaatgtta aatatactct attttttctt
    44341 ttagagttat gcaataataa atatgccatc acaacaaaga atgaaaccca ttattatcta
    44401 gtctatctcc attctttttg ttagcaggag ttgtggtggc tttccgtacc cttagctcag
    44461 ttggttagag cagacggctc ataaccgtcc ggtcgtaggc tcgagtccta cagggtacat
    44521 taatgtagcc atttgaatcg ttctgtgtta ga
//
LOCUS       DAYYNG010000004        42550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000004 DAYYNG010000000
VERSION     DAYYNG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.004"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1052)
                     /locus_tag="ACHBFA_000196"
     CDS             complement(12..1052)
                     /locus_tag="ACHBFA_000196"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9393723.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(1329..3131)
                     /locus_tag="ACHBFA_000197"
     CDS             complement(1329..3131)
                     /locus_tag="ACHBFA_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9393724.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3145..3897)
                     /locus_tag="ACHBFA_000198"
     CDS             complement(3145..3897)
                     /locus_tag="ACHBFA_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393725.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3906..4268)
                     /locus_tag="ACHBFA_000199"
     CDS             complement(3906..4268)
                     /locus_tag="ACHBFA_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9393726.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4444..5316)
                     /locus_tag="ACHBFA_000200"
     CDS             complement(4444..5316)
                     /locus_tag="ACHBFA_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9393727.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5342..5854)
                     /locus_tag="ACHBFA_000201"
     CDS             complement(5342..5854)
                     /locus_tag="ACHBFA_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9393728.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(5992..6783)
                     /locus_tag="ACHBFA_000202"
     CDS             complement(5992..6783)
                     /locus_tag="ACHBFA_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM9393729.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(6838..7446)
                     /locus_tag="ACHBFA_000203"
     CDS             complement(6838..7446)
                     /locus_tag="ACHBFA_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9393730.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7439..7756)
                     /locus_tag="ACHBFA_000204"
     CDS             complement(7439..7756)
                     /locus_tag="ACHBFA_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9393731.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7912..8640)
                     /locus_tag="ACHBFA_000205"
     CDS             complement(7912..8640)
                     /locus_tag="ACHBFA_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM9393732.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8799..9680
                     /locus_tag="ACHBFA_000206"
     CDS             8799..9680
                     /locus_tag="ACHBFA_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9393733.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9917..10960)
                     /locus_tag="ACHBFA_000207"
     CDS             complement(9917..10960)
                     /locus_tag="ACHBFA_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9393734.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10963..11631)
                     /locus_tag="ACHBFA_000208"
     CDS             complement(10963..11631)
                     /locus_tag="ACHBFA_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9393735.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11773..14109
                     /locus_tag="ACHBFA_000209"
     CDS             11773..14109
                     /locus_tag="ACHBFA_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM9393736.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14224..14535)
                     /locus_tag="ACHBFA_000210"
     CDS             complement(14224..14535)
                     /locus_tag="ACHBFA_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393737.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14716..16191
                     /locus_tag="ACHBFA_000211"
     CDS             14716..16191
                     /locus_tag="ACHBFA_000211"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM9393738.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16238..16822
                     /locus_tag="ACHBFA_000212"
     CDS             16238..16822
                     /locus_tag="ACHBFA_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM9393739.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16824..17243
                     /locus_tag="ACHBFA_000213"
     CDS             16824..17243
                     /locus_tag="ACHBFA_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM9393740.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17524..17808)
                     /locus_tag="ACHBFA_000214"
     CDS             complement(17524..17808)
                     /locus_tag="ACHBFA_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM9393741.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18161..18871)
                     /locus_tag="ACHBFA_000215"
     CDS             complement(18161..18871)
                     /locus_tag="ACHBFA_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM9393742.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18868..20340)
                     /locus_tag="ACHBFA_000216"
     CDS             complement(18868..20340)
                     /locus_tag="ACHBFA_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9393743.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20478..20951
                     /locus_tag="ACHBFA_000217"
     CDS             20478..20951
                     /locus_tag="ACHBFA_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9393744.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21104..21655)
                     /locus_tag="ACHBFA_000218"
     CDS             complement(21104..21655)
                     /locus_tag="ACHBFA_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9393745.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21760..22290
                     /locus_tag="ACHBFA_000219"
     CDS             21760..22290
                     /locus_tag="ACHBFA_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM9393746.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22773..23216)
                     /locus_tag="ACHBFA_000220"
     CDS             complement(22773..23216)
                     /locus_tag="ACHBFA_000220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9393747.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23232..24392)
                     /locus_tag="ACHBFA_000221"
     CDS             complement(23232..24392)
                     /locus_tag="ACHBFA_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9393748.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24899..26599
                     /locus_tag="ACHBFA_000222"
     CDS             24899..26599
                     /locus_tag="ACHBFA_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393749.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26701..27534)
                     /locus_tag="ACHBFA_000223"
     CDS             complement(26701..27534)
                     /locus_tag="ACHBFA_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9393750.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27838..30003)
                     /locus_tag="ACHBFA_000224"
     CDS             complement(27838..30003)
                     /locus_tag="ACHBFA_000224"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9393751.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30263..32557
                     /locus_tag="ACHBFA_000225"
     CDS             30263..32557
                     /locus_tag="ACHBFA_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM9393752.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32550..33215
                     /gene="pgmB"
                     /locus_tag="ACHBFA_000226"
     CDS             32550..33215
                     /gene="pgmB"
                     /locus_tag="ACHBFA_000226"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9393753.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33208..34215
                     /locus_tag="ACHBFA_000227"
     CDS             33208..34215
                     /locus_tag="ACHBFA_000227"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9393754.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34237..35256
                     /locus_tag="ACHBFA_000228"
     CDS             34237..35256
                     /locus_tag="ACHBFA_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9393755.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35537..36904)
                     /gene="nhaC"
                     /locus_tag="ACHBFA_000229"
     CDS             complement(35537..36904)
                     /gene="nhaC"
                     /locus_tag="ACHBFA_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9393756.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(37013..38434)
                     /gene="tyrP"
                     /locus_tag="ACHBFA_000230"
     CDS             complement(37013..38434)
                     /gene="tyrP"
                     /locus_tag="ACHBFA_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM9393757.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38677..40554)
                     /gene="tdc"
                     /locus_tag="ACHBFA_000231"
     CDS             complement(38677..40554)
                     /gene="tdc"
                     /locus_tag="ACHBFA_000231"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9393758.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40845..42101)
                     /gene="tyrS"
                     /locus_tag="ACHBFA_000232"
     CDS             complement(40845..42101)
                     /gene="tyrS"
                     /locus_tag="ACHBFA_000232"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9393759.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(42213..42418)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        12906 a         8805 c         7547 g        13292 t
ORIGIN      
        1 tttaatttat tctatttctt tatatttatt ttgtattccg ttacaaactc gcgtacttgg
       61 ttaggttcca gagtgacatc tccaaaactt tcaagtcgct ccgctcctgg cagttcttga
      121 gtctcaaatg tgataccgcc atgatttact aatttattcc cgcgcatttc cggtccatct
      181 tctccaaaat ttgctgtaaa aatcacgatt cctgaagcat ctgtttttac ttggatcgat
      241 actttttcat ctgggcttgt caattccgca gcatttagta aaccagattg ttttttcaac
      301 aaaaatggat gatcgatacc gcccactatc attgattggc gaaaatcact ggcaaagacc
      361 tcacctaatt ttttaggacg ttggaagtca aatgctgtgc cacttactga aactacttct
      421 cctgtaggcg ttgtatcttt attgagcgta gcaaataatc ggctattcaa ccaaagtata
      481 tgttcgtcga ttgactgtgc tggatcaccg gttaaattga aatagacatg atttgtagga
      541 ttgaatactg ttttgcggtc actgatccct tggaccgtca ctcgccatgt atcatcgttc
      601 gtcagcgtat accgtacttc tacatctaaa gttccaggaa atccgttttc catgtctggg
      661 ctagtctgat agaaaatgac cgatgcttcg ttttcaccat tgatgactgt ataatgccac
      721 tgcttggctt caaagctagg tgcaccacca tgtaaacaat gaccagtgtc cgggtctgtt
      781 tttacttcga acgtttcatc atctaatttg aatgtgccat tcgtaatccg tccagccata
      841 cgtccgattg aagcaccgat ataagggtct ttttctagat attcttttgc ttcatcaaaa
      901 cctaagatca actctcgttt ttcattatcg attattgttt gaaaatgaac aacccgtgcc
      961 ccaagatcac tgacagacaa agatgtccca ttttgattcg tgatcgtaat caagtgatag
     1021 ttttcgccaa aattttgttc attgatttcc atgttccttg caccctcttt tctttcattt
     1081 gcactgattc cagtatacat ggaaaccgtt ttcttaacag cttattccgt gagtaacgag
     1141 aaaaaattta ctatattcta ataaaagacc gtgaaaaaaa ggttgtgaca aaaatattgt
     1201 cacaacctct ttttccgaac aaacggtgtt caaaagcttg catctgcggt aataagccca
     1261 accaaacgaa aaataggagg agctattttt gcttggctgt tcttattact tgatgcagga
     1321 cagctttttc acaatctctt tttcaatacg attccagtat ataaccatgt cgtcagtaca
     1381 tagtagatga aatacaatac gaagaagatc gtaaacggaa cccaaatatc tttgtatgga
     1441 ttttgcatga atagaccaaa catttgtaat ccgaataaga catggactac acctaagatt
     1501 cctggtgcta agaataacac gcctacttct ctacggatcg attgtttaag caacccgcgt
     1561 cgtgttccta tcttttcaag catttcataa cgagcaatgt cactcttgct tcctgacaag
     1621 atcttgaaca tcaaacagct agcaagcatg gtcaagaaag ctaaacctag gaagaatccc
     1681 ataaattcaa aaccagagaa tagaccatta tacacttcat acatttcata tttctggttg
     1741 ctgtactcta ctttttgcaa agatggattg ttcattttat tttgagcaac aagtgtttcg
     1801 attggtttca ggtttgcttg gaagtctttt acctgaacta gctgtaaagt ggattgtggc
     1861 aagttcagtt gatcaaattc tgattgactg actaccttga tttctttttc ttgctgttcc
     1921 ggcaattcat agcttcgaag ttcctcttgt acagtcagtt cattcatttt ttcagcagga
     1981 actttttctt tcgtgatctt cggcggtgtt ccatcttcat gttttatcat gatcaatggt
     2041 tgagccgcaa attcacttgc attatagtaa atcgttcccg catcttcttt ttgagtataa
     2101 gaagcagaaa gtgttggtga aagttctttg attttttgct gatcttctgc ccgaggatta
     2161 ttcatcacta gatcatatcc agaattgttt tctgtcaagt ctgcaatttc atttttaaaa
     2221 cctaatccta ccgtcaaagc gcccagtgcc aaggcaaaca gcatagcgac cattgagagc
     2281 atttgtgtat attcacgaat acgaaagttt aactgtgaca atgtgaaatt gttcaatttt
     2341 ttcaatgaaa tctgttctga tttctttagt aatgataaaa agaaaatgat cacagaatgg
     2401 aaaatgaaat aagttcctaa tacgatcgta actaaggcta ctcctatacc aaagacttga
     2461 taggtcccca cgtgtttcat catatagtaa ccgcagccta gaaaagcaat tcccagtact
     2521 acttcaagga aaaacaagaa tttatgctgc ttgattcttg ttggtgtctg atccgcacga
     2581 atcaatgcta atatcggttt tttgacaatc gaaaaagcat tgacaacagc agctaataaa
     2641 aacaaaacag caaaaaacaa aacagtgatc aacaacccgt gaatactaaa aggtgtaaaa
     2701 tgcgaaatat tgagatctaa ctgatccact aataatcggt ggacgatact tgtcaatcct
     2761 acaccgacag ctgaacccac taaagtagct gcgatcccta ctaagaatgt ttcagaaaaa
     2821 atcatctgtg cgatttttct gccttttgct cctagcatca taaacattgc atagtctttt
     2881 tgccgcatcg tcatcaaaaa ggaatttgca tataaaatat aaacgaatgt gatgatccct
     2941 agtaaaacag aaccgaatcg gaaaatgata actgtagccg caacgatcgt attacttttc
     3001 aaaaaagctt cgttgcttgc cattgattca aacatgtaaa aaatagcaga tgccataact
     3061 aatcctgaga ataacaccag gtagtctctt aaacggctct tgatcccagt aagtgataat
     3121 ttccaaagca tagtcaatct ccccctactg ttccaagttt ccaagaatag atagtatttc
     3181 tcgatagaag gaatcacggc tttgatctcc acgtttcact tcttgatgga taccgccatc
     3241 cttgatgaat aaaatacgtt ggcaataact tgcagaaaac ggatcatgcg tcacaagaag
     3301 aatcgataca tgatcttttg tattcaattc gtccatcgta tctaaaagat cccgggcact
     3361 tttggaatcc agcgcacctg taggctcatc ccctaataga atcgttggtt gtgtgatcaa
     3421 tgcacgtgcg gcggcgaccc gttgtttttg tccaccagaa atctcagaag gatagctttc
     3481 caaaatatga gagatggata aacgttctgc aattttttgg acacgttgct tgatttcttt
     3541 tggtctgacg ccttgtaaag aaagaggaac agcgatattt tcttgtgctg tcaagctttc
     3601 taataaattg aagtcttgga aaataaagcc aatctcattc gcacgaaaat ctgctagttg
     3661 atttcctttt aatgtggtca catctttttg attgattcgg atatgcccat ctgttggttt
     3721 atctaatgtg gataaaatat tcaaaagtgt cgatttacca gaaccagaag cgcccatgat
     3781 cccaataaat tcgccttttt ctacatcgaa gctgatgccg cttaatgctt gcgtcttttt
     3841 ctcattgttt tttccgtata cttttgttac attctgtact tcgactattt tgttcatttc
     3901 tttccctacc ctttaattag tttttccagt gctgttttcg gaacgtcttt tttttcgact
     3961 tcttcatacg aagtgacacc tttatttttg ttccaggtga tttttaaata tgctccttta
     4021 cgaagcggtc gagtcaattg tcccttgaag tccagttctt tttccttacc ctctttcgta
     4081 tagccaggca ggatatatct atagttgact gtttcttgtc cagagtctga ttttgaaact
     4141 gtttttgtac catctgtcgt aatttgtaca tagtattctt ctccacccat aactacgtaa
     4201 tctgcgattt tcacccctgc aagtaccaca acgatgaaaa caagtagacc gatcagaagt
     4261 tttttcactt tttccccttc tctcttactt tatatactga ttatagaaaa aagcgaacaa
     4321 ttctgccttc gaatcaacta tcattcttct ttcaattttg tcattttctt taaaacaact
     4381 aaaaaaagag ttcaagacaa taatccgtcc taaactcctt cttcctatct tacaaaaagt
     4441 atgtcactta cggtctttca tgtcgcctgt attgataaca ggttgcgttc ctttgtctgt
     4501 aatgattgag acaagtaaat tattgatcaa ttggactttc cgatcatcgg tgaaattgat
     4561 ttcttgtcca tcttcgattt gctctaaggc catttgagtg attgtgacag ctccttcaac
     4621 aatggtttgt cgagcagaca aaatggcttt tgcttgttga cgttggagca tggcactagc
     4681 gatttctgtt gcataggcta aatggttcaa gcgtgtttcg attacttcga cacctgctac
     4741 tgctaaacgc tcttgcagct ctttttgcaa ctcgtcagac actgccgtcg tgtttcctcg
     4801 aagcgtcaaa tcattctcat tgaatgtatc atacgggtat tggctagcga tatgccgaat
     4861 ggctgtctca ctttggattt ctacaaaatc ttgataatac gctacatcga ataatgcctt
     4921 tgctgtatca acgactttaa agactactac tgctgagatt tcgatggggt ttccatccaa
     4981 atcattgact tttaaaacag aactattgaa attccgaact ttcaacgaaa cagtcatttt
     5041 ttgtgccaaa ggtatcgtaa ggaaaaagcc attttctcga atcgtaccta aatattgtcc
     5101 aaaaaacaaa attacttttg cttgattagg actgactaca gttgccgaac ttaacaacaa
     5161 taacgtgatc aaccaaagaa aaatacttaa aaaaagtgcc caaactttcg cttgccacat
     5221 gcctaaatag aagaaaaaca atccaactaa agctaatacg accaatccga caatccctgc
     5281 ataaccattg atataaaatg ctcttttttc tctcatgcta cttcctgcgc tcctttatcc
     5341 ttcaataaat atctaatgtt acttttttaa ttttttttcg aatgcgcgcc attacacctt
     5401 ctagttctaa ctctttgtct tcagtgatgt accaaccgct tgcctgtgtc cctccggctg
     5461 cctgcggtaa ataatccaat tttgttcgaa cccagtgctt ttcttctgta aaatagtctt
     5521 ctaccaagac ctctagatcc atccctgctg aaagaggcaa atcttcccga acagatttaa
     5581 agataaatcg ttccttcttc ccatcatact tcaatattcc tgtatcgata atcggtaaag
     5641 aataataatc cagctctttc ttcaccgtca ataaatgttt gaaaacttca tggtacactt
     5701 gctctaattg gcgaccttct gaattgttcg ggtaattaac catcactttt tctttgtctg
     5761 gatcatagaa ggtttggctt agttgagtga tataagtatg taatctttcc atttctgtct
     5821 ttaacttttc tctttgacct tcttctacct tcatatactc tttcaccctg ttttcttttt
     5881 agatattttc tttcttattt tactatagtt atgtgcgtta agcgatgaaa aacagcaaag
     5941 attggaagga tctttgctgt tttttcttaa aatatccagt tgagtacagg atcaatctgt
     6001 ttcattgtcc tcttgatccg tcgtaatatt gatcgatgtg tctttagcag tgatctttag
     6061 ctttttctgt ccttgttcta acaaagtatt tgttattggc tggtagtctt tttcaatata
     6121 agattcacca gacgtttgga tagtatagcc actgttttcc atctcatgca aagataaatt
     6181 accaccgttt aggatgactt ttccttcttt gctgatcaaa ctgtcttcta gatggaagtt
     6241 gctgttttca cctgtgacga tcaagttatc cacattggcc tcatctagtc gaagtgagcc
     6301 taattcatct ttcgcagtta ttgttcctcc tgtaaattca ttgaacgata cccagccatc
     6361 atgtgtctta gtattcactt ttttgattga tacttcgttg atatcaattg cactattatt
     6421 cccttcgatt gtcaattcag ataattcatg atctgcaggg acaacaatag tgatttgtgc
     6481 acttgtgtct tctaaactaa tgattgcacc tgttgatttt ttgactgcat tcacagtcaa
     6541 cacgccattt ttctccttta tttctggctt ttctgtctta tcataagtcg tttttatctc
     6601 aaaatccaat ccgcgtatga ccgaaactgt ttgattttct gcatggatga cgattttgtt
     6661 tgcatccttg attttttttg tctcagtaac catatcgatc aaccgaggac cattatccca
     6721 agtcagactc ttttgagcgc ctaatgtata agttgcacct gctaggaggc caccagtaag
     6781 catcagacca ataccggtaa tcaaaaaatg tttcaatctc aatttatgtg tcctccttca
     6841 aacgcccgct gaatgcagat ctctttttgt aataaaacgt cgtcccatcc atttaacaaa
     6901 gttcatcatg acataaaaaa gcccttttgt tcctttcagc aacagtggcg aaataaatac
     6961 tgcgcttcct gtggcgaaca gaccgacacc gataaagaat aatccactca gaatcgattg
     7021 tccaaacaca ctgattcctg agatgatcat gaataggcca acacctaaga tactgataca
     7081 taaaacatag atactaaaaa tgagcgctag taaagcaacc aatagtcccc caataaccaa
     7141 tataaacccc agtgctagtg gaatgaggac aggagaagca aataaaccga taatgattaa
     7201 ccaaatcagg cgaatttgtc tttttggatt tttcactaac tcgttttctg cttgttcttc
     7261 ttctgaaaag taactgattt ttaattttaa ggcaaactgc tttggtgtac catattcatc
     7321 aatgatctga tctaatgttt ttccagaatc taaaaaatat tcctcataat actggatcaa
     7381 atcaaaacga tcgtcttcag gaatttcttg aagatgatcc acaacttcat tcaaatattc
     7441 attcatttgt gctctcctcc aataaccggt ctaaagaatc tcgaaaaaca atccattcat
     7501 caataatttc ttctaaatga cgacttccca ttggtgtcaa cgagtaatat cgacggtttc
     7561 tgccttcaaa aggttcatca taggtctcaa gatatccttc ttttttcaac cttctcaaaa
     7621 ccggatatag tgtagattca ctgacagaga gactttcttg tacgattttt gttaaggcat
     7681 acccatacat atcttccttg cttaatgctg ctaacacagt accatcaaga agaacagttg
     7741 gaatctggat gtgcattacg tcaccttcta ttctcaagtg taatatttta tttcaatcta
     7801 tattatacaa catataatat agtttcaaaa tcagtttgtc aaatacaaaa aaactgacta
     7861 aaagatttga tcctttttag tcagcaaaaa atggcttctc tttgattttt attataacag
     7921 tggcataatc caatttgtcc acatccagct cagtggcatg ataacgatca ttgcaggtat
     7981 catgtcagct actgggaaca ttttcacgtt gatcattcta aaaccggtag cgaccatcaa
     8041 gaatccgccg caagctttaa aatctaagat catgtctggt gtagtcaatg gataaataaa
     8101 ttttgccaat aagaacaaaa tataaaaaat aatgaattgg ggaattgcta tcacagaaac
     8161 gacataccct aaattacacg caaaaatagc tgctgtgaaa aaatcaagga cagatttaga
     8221 aatcaaaatc gtactgtctc ctgtcatccc agaatctaaa cttccataga tccccgtacc
     8281 acttgcacaa aatagaacaa tgaccgtcac taatgtgctg ataaattcat cgtgggtcat
     8341 accaagcttt tcactaggga agattttaga aataggtact tgcatcagcg cagccccctt
     8401 atttatccca ttacctaaat gaacaatcaa accaatcgct gttcctatca ctaaagcaaa
     8461 aataacagcc ggcatatttt tcatcggcgc aatggaatag attcccatcc ccatcgaaca
     8521 cacaccaaaa accatcgtca gctgtgcttt aaaatgttca cttaatttat ttcctactaa
     8581 tccgccaatt agtccgccaa acaaaacgga acctacattg atcaaaactc cagttggcat
     8641 caaaaaactc ctctcttttt ctacgaagcg aaaaaaatca gaaaaatttc tgtaattatt
     8701 gctttcgctg caagaaagat tgtaacagac agagaagatt acaccaattc attttttgtg
     8761 ttaaagtatt ctttattgga ataagtagga ggaatatgat ggatatccat aaattcgaag
     8821 tttttcttga tttagccgaa acaatgaatt tcaccaaaac tgctgatcga caattcacca
     8881 cccaaggaaa tatttctaaa cagattcttt cgttagaaac agaattagac gtcaaattat
     8941 tcgaacgcgc ccatagaaaa atcgaattga ccgaagctgg aagcttattg ctcccatatg
     9001 taaaaaatgt cgtagaacaa taccatgcta tgcaagaagt gcttgaaacc tatacgaaag
     9061 acaacaatct ttcattaaat atcttaacga taccgacaat gatcaattat aaaggttttt
     9121 ccaaaatcac cgagtttttg aaacgccatc ctgaatttac agtccagttg aaagaagtgg
     9181 aaagtgtcga attaacttac aatgacgcca atgaacatag tgacacgatc cactttgcta
     9241 gaagtttcca ggccccacca agttcgattg agtggttgcc tactgaaaca gacgattttg
     9301 tagcagttct acctaaaaat catcctttag ctagtaaaaa gaaattggat ctaagcgaac
     9361 tgaaaaagga gaactttttg ctccttggcc ccactacgaa cttgtatcaa cctgtactag
     9421 atctgtgtcg cgaagctgga tttgaaccaa aaatctctta taaaggagca agaatcgact
     9481 tgatcatcaa tatgattgca aatgacatgg ggattgctat tgttatggaa aaaacagtca
     9541 agaatctatt aaaagaaaat actgtgattc tccctatttc tccaacaaca gagagttatc
     9601 ttgcatttac tcgtaaagtc ggtgagcatt ctttggcttc agatacattt tgggagtatt
     9661 taaatcaaga agaaaactaa gccctacatt ttctgtccat ctatccaccc aaaaaaagag
     9721 agcgacgcat gactttcgct ctctttcttg catcatgtcc ttgtttcaat gacacctcta
     9781 acatttctcg tagttcaata agaaagcagc gatttttatg ccaaaagaac cgtaagaaca
     9841 tttccctaat cacaaaaaaa cgactaaaaa cccctaaaaa ccttcaatag aatttttagg
     9901 ggttttattc tttttcctat tgtttactta accgtatcat tgctgtcgtt cctactcctt
     9961 cgatactgct ataatcaata cgtccatcat gcagttctag aattttttta accagtgaaa
    10021 gtcctagccc attcccagaa atttgacgac ttgtatctcc ttgataaaat ttttcaaaca
    10081 ttcgatctct ggtctcttca ttcattccta cacctgaatc ggtcacttcc agtacaatct
    10141 ctgtggctgt ctcaaatagt ttgatatgaa tctgtccatt gatttgattg tactttatgg
    10201 cgttatccat gatattcagc cacacttggt acaaaaactc ttcattgcct gtgtaattta
    10261 ctgcagcaag ttcgatatcc aattctagct gttctttctc ccatttaggc tgaaaaaaaa
    10321 cgattacttc tctgatttgt tcatctaatc gatactcttt cttttccagt tggatacgct
    10381 ggttttccag tttgttcaat tttaagatgt tctctgttaa ttgtgaaagc tgcgtgatac
    10441 tttcgataat ccgatataaa aatatttggc gttcttcgtc agagagattt ggggcttgta
    10501 acaattgaac atatccttgg attgttgcta gtggtgtttt gaattcatgg gatacactgg
    10561 acacaaagtc attcctcagt gtctcgatac tattgagttc ttggaccata acgttgaaat
    10621 ctttatacag ttgctgtact tctgctactt tctgctgttc atccattcga atcgaaaaat
    10681 ctcccgttgc taccagactc atatttgtcc ctagcgcact gattggatgc aggatacgcc
    10741 gcccaacaaa aatcgatatt cctgtaccca cgaccatact gaaccccgca aagacgaata
    10801 acgggtataa cgttggtttt ccagccgatt cagcaggcga gatagaatgt cgagacaata
    10861 agaaaataaa accgagaaaa cacagcagtg tcactaagat cgaaatgaag acaatactag
    10921 caaaatagat ccataattgt gaaaatagct tttttttcat ctttaaataa ccgccttata
    10981 gcctagtccc cgtactgtga tgatttgaaa atcttccgtc ttttcaaaac gggtccttaa
    11041 acgtttgata tgtacatcga tcgttcgttc ttctgtattc gtatctaatc cccaaatatc
    11101 atccatcaac tgttggcgag tgaagatctt gttgggataa gacagtaatt tatacagaag
    11161 cagaaattct ttttgaggca gaatcaacga atgctgatct tgtaacactg tataagcatc
    11221 tagctctaat tttgtttgtc cgattatcaa gctactgctg taattgatct ttgaacgtcg
    11281 aagcaatgct tccacacgta ggatcatctc attgacatcg atgggttttg tcatataatc
    11341 atctgctcct aatttgaacc cgcgtttctt atcctcaaaa tcggcctttg ccgtaatcat
    11401 tagaacaggt gtgtcaaatc tggtgctacg taatgagtct agcaagtcat agccatccat
    11461 tttcggcatc atgatatctg taatgattaa ttctatacgt gtcgcttcta gttttttcag
    11521 cgcatcctct ccattcaatg cggtgatgac tgaaaagccc gctcgttcca acacagaacg
    11581 gtaaagcaac tgtaattgcg gatcatcttc tactactaaa atacgattca ttgatctccc
    11641 tctgtttttt gaaaaatttc tagatttttt cttgtagttc atcttcagtt aacatttatt
    11701 tcgtatacta cgatcattgt tctgatatac ttattagtat accgaatatt tcaatacggg
    11761 agaagggtga ttatgcttca attaaaagat ataaaaaaat attataaagt cggcgaaacc
    11821 accaccaaag cattggatgg tgtgtctgtc gctttcagac aaaaagaatt cgtagcgatt
    11881 ttaggtccta gtggttctgg taaaacgact atgctgaatg tgattggtgg actagataat
    11941 tatgattccg gtgatatggt aatcaacgga aagtctacta aagactttaa ggacagtgac
    12001 tgggatgcct accgaaacaa ctctatcggt ttcatttttc aaagttacaa cttgattggt
    12061 catctaggca ttattgaaaa cgttgaactt gggatgacat taagtggtgt ttctaaagac
    12121 gaaaaacgaa aacgggcaga agatgcattg caccgagtcg gcttgacaaa tcatatgcat
    12181 aaaaagccaa atcagttatc tggtggacaa atgcagcgag tggccatcgc tcgagcacta
    12241 gccaatgacc cagatatcct tttgtgcgat gaaccaaccg gagcgttaga tacagaaaca
    12301 agtatccaga tcatggaatt gatccaagag ttatcgaaag aaaaattagt gatcatggtc
    12361 acacataatc ctgaactggc ccatcaatac gcagatcgaa tcattgaatt ttctgatggt
    12421 aagatcttaa cagattccca tcctcacatc gaacgaccaa aagatgatca atttaactta
    12481 cgcagaacaa agatgagttt ttggacagcg ttaaagcttt ccttcaacaa tatccgaaca
    12541 aaaaaaggac gtactttctt gacctccttt gcttccagta ttgggatcat cggaatcgcg
    12601 atcgttctgt ccctttcttc tggattccaa aaacaaatcg ataacacgca agctgaaacg
    12661 atggcgaaat ttccgattac tatttctaag gtaacgacta accagactcg tgacgatgcc
    12721 ggattgggtg caagcaaagc ggattatcca gacagcaaaa cgattactgc aaaagtcagc
    12781 gaagaagata aagcacagca cacgaataag attgatcaaa catacgtcga ttacgtaaca
    12841 gacatcgatc ctaacttaag taataatatc ggatttaccc gtactacagg aatcaatctt
    12901 ttacgtgatg taaatggcaa agtacagcct gtcagcttct cgaatcaaaa tcctgatacg
    12961 gaatctcttt ctttatctag tacgatgtca gcgatgacag gtgtgggagt gtcaagtttc
    13021 cctacacagc ttgacactag caaagaaaac tttttaaaag acaattattc tttacttgcc
    13081 ggaagttatc cagcctcagc aacagatgtc gtgctgatcg tagacggcaa taataatact
    13141 aatatcaacg cattgaagaa tcttggattt gatgtgaaag aagacgaaaa acttgatttt
    13201 gatgatatcg ttggtacaac attcaaatta gtaaacaaca atacttacta tacaaaactt
    13261 ccaactggga attttatccc taatacagat tatgatgcga tgtatcagaa tgcttctgat
    13321 gaattgaaga tctcggggat cctacgcgtg aaatcttctt ctacaatgaa tctcttatct
    13381 ccaggaatcg cttatagcga tcaattaacg actcaaatcg tcaacgaaaa caaagaatca
    13441 gaaatcgtca aagctcaaag agacagtggt gtaaatgtct taactactga aaaagttgac
    13501 gaaaatgcca aacaaacatt gcttagttat ttaggtggag acagcttgcc ttcaagtatc
    13561 atgatttatc cgaacaattt tgaagacaaa gaaaagatct tggattatct agatgattac
    13621 aataaaggca aatcagatga agataaaatc atttatactg atttagctgg aacgatgact
    13681 gaactgactg gcggattgat ggatgctatc acttatgtcc ttattgcttt tgcagggatc
    13741 tctttagtaa caagtatgat catgatcagt atcattactt atacatctgt gattgaacgg
    13801 acaaaagaaa tcggtgtact aaaagcttta ggtgcacgta aaaaagacat cactcgcgta
    13861 tttgacgcag aaacgtgtat tttaggtatt tcttctggga tattaggtgt gtttattgca
    13921 tggctagcta cgttccctat caatagcatc ttatacaaca tgacggatct aaaaaatgtt
    13981 gctcaactaa atcctgtcca tgcgattatt ctagtgatcg tctctactat attgacgatg
    14041 cttggcggtc atcttcctgc acgaatggca gcgaaaaaag acgccgctat cgctttgcgt
    14101 gcagaataag gttgtgacaa ccacatttag atttgagtca taagagcgct gaaatagtag
    14161 aaaacagcaa gaaacgagga agtaatatgt tccttgtttc gcgctgtttt ttttaattat
    14221 tttttataat tctggtatac ttttctttcc tttgtaccac aactcttctg gcgtatacct
    14281 gtcttttcca tttatgaata ctgttccatc tgcttcaatc gttacttcag tcccaatcca
    14341 catattacga gaaagtgcga gaagattttt tctttttcct cttccttttt ttccaaattg
    14401 caaggcatcg gttatctctt ctgtccaatg agatttcacg tattttactt ctccgttttc
    14461 aaatgtaact ttcaaaatat tcgctatatt ttccactttt actgctttag gaaatgactc
    14521 ctccattttt ttcactttcc ttcctccaac actaagtatg cgctttcttt tagtgataag
    14581 tatatcatag tttattctat tatcaaaaaa acgcatccat tccattgtag aatactttac
    14641 cgaacaagtt gaattgtttt cctctttttc accttttact atagataggt aaagacaact
    14701 aaggaggaat ctctcatgac agatcaaccg tatgatttaa gaaaagtcat cgaagaacta
    14761 aaaacacttc caggtcaata tcacgaaaca gatattgaaa tcgatcctga agcagaatta
    14821 tctggtgttt accgttatat cggtgctggc ggtacagtaa aacgtccgac tcaagaagga
    14881 ccagctatga ctttcaacaa tatcaaaggt ttccccggca cacgtgtgaa catcggaaca
    14941 atggctagtc gtaaacgtgt aggtgaaatt ttgcaccatg attatcgaac acttggtcgt
    15001 ttcttgaaag acgctgtaga aaatccaatc aaacctgtga aagtagacaa agcagacgct
    15061 ccagcacaag aagtcgttca tctagctacc gatgcagact tcgatattcg caacattttg
    15121 cctgcaccta caaatactcc tgaagatgct ggtccataca tcacaatggg tgtagtctta
    15181 ggatcagatc cagaaaaaac aatgagcgat gtaacgatcc atcgtatggt tctagaagat
    15241 aaagatacga tcggtatgta catcatgcct ggcggacgcc acattggtca cttccaaaaa
    15301 caattcgaag ccttgaataa accaatgcct atcactatca atattggttt agatccagca
    15361 attgccattg gcacgacttt cgaaccacct accactccac ttggctacaa tgaactatgg
    15421 tgtgcaggag ctttgcgtaa cgaacctatt caaatcgttg atggtatagc agtcgatgaa
    15481 gtgggtattg ctcgtgctga attcatcatt gaagcagaaa tcatgccaaa tgaaacaatg
    15541 caagaagata tcaacacgaa ctcaggaaaa gcaatgccag aatttcctgg ctatactggg
    15601 gatgctaatc cagctgtaca agtagtgaaa gtcaaagcag ttactcaccg aaaagacaat
    15661 ccaatcatgc aaacaacgat tggtcccagt gaagaacacg tatctatggc cggtattcct
    15721 acagaagcaa gtattcttgc cttggtcgat aaagctattc caggaaaagt agtaaatgtc
    15781 tacaatcctc ctgcaggtgg tggtaaattg atgaccatca tgcaaatccg taaagaaaac
    15841 gaagcggatg aaggtattca gcgtcaagca gctattcttg ctttatcagc cttcaaagaa
    15901 ttgaagacag tcattttagt cgatgaagat gttgatattt tcgacatgaa cgatgtagtt
    15961 tggactttca acactcgttt ccaaggagat aaagatatca tcgtgatccc aggaatgagg
    16021 aaccatccat tggacccatc agaattacca gaatataatc ctggctctat tcgtttccgt
    16081 gggataagtg caaaaactat ctttgacggt acagttcctt tcgatatgaa agaacatttt
    16141 gtacgtgcaa aattccaaga agtagaagat tgggaaaaat atttgaaata atgaatgatc
    16201 tgcaactgtt ttgacatcat aaaaaggagt gcggaaaatg gaacaattgg cacaacggaa
    16261 gaaaaaaatc gtgattggtg taagtggcgc ttcaggcacc atttatgcca tcaacctagt
    16321 gaagaaacta aaagaatatc cgatgataga aacacacgga atcatcagtc catgggcaaa
    16381 acagaactta aagctggaat ccgacttatc actaactgaa ttcgaaagcc tgtttgatta
    16441 ccattattca aacaaagatt taggtgctgc tgtcgccagc ggatcatttt tgaccgatgg
    16501 aatgatcatc gtaccggcaa gtatgaaaac agtcgcaggt atctctgttg gtttaggcga
    16561 caacctgatt gcacgtgctg ctgatgtaac aatgaaggag caacgaaagt tagtcattgt
    16621 tcctcgtgaa acgccattaa atgccattca cttggaaaac ttgactaaat tagcacgact
    16681 aggtgtgcaa atcataccac caattcctgc tttttataat cagcctcaaa cgatccaaga
    16741 tcttgttaat caccatacaa tgaaactttt ggatcaattt gaaattgtaa atgagcattc
    16801 aaaacgttgg cagggtgatt aatatggaaa aagaaacgat agttgaagta accaaactag
    16861 gtttcacgat ccaagctgct gttaaagtaa tcggtaaaga cttattgatc actttaacag
    16921 gcggagatac accacatatc ggaacagtca ctacattttc tcgtgataca gagatgcaaa
    16981 ctgtacgttt ccctagccac gatggtcggt tccataaaga cgatgtctta gccgaaaaaa
    17041 tagctgtgat catcaaaact gccgttccgg gaagttgtac gatcacttcc ggtatccatg
    17101 ttgaccatat ctccaatgca caaatcaacg cctcttttcc gatgtcagaa gagttaggtt
    17161 acaagatttt agattggttg gaaaaaatag atttccatat caaagaacca gtttattata
    17221 aagatggtga aaaaccatta tgaaagaaga ggttgtgaaa tcagcgttat gacctgagta
    17281 tgagagaaaa aaatggaaaa atagcttctc atattttgcc gtttttgagc taataccgag
    17341 ggacagctga ttgaacgccg tttatcaaga ggttgtgata aagccgcttc gtttcgagta
    17401 gtaagaaaaa ttttgtaaac atgggtttcc acatttttgc ataaatttga cttactaccg
    17461 agaaactggc ttttgaacac cgtttatcaa gaggttgtga cacgtctttt gtcacaacct
    17521 cttttatttt tgcttcactg taactgctac aatatcaccg atttcttttc cgatgttttt
    17581 acgaatatct tttcgtagtc caaggatgta gcagactgaa ccatctggat ttttcacgcc
    17641 catattcacg atactgcctt catacggcac attgtcaaaa gttgcttgga ctttaactct
    17701 tccagcaccg aattcttttc ttatatcata cggaaaaata atatatgccc cgcccttgcc
    17761 gacttcggaa gcatagattt ttgattcaaa agaataaatt tccctcattt tatttctcct
    17821 attttttaca tctagtatag catagcaaaa aaaaagaagg aactgtgaca aaagtcttgt
    17881 cgcagttcct tttttcgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    17941 atagtcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttaaac
    18001 ggcaggctca caacctcttc cttaaacggt gttcaaaagc ttgcatctac ggcactaagc
    18061 ccaatcagat gaaaaatatg gagtgctatt tttatctgat tgttcttact gcttgatgca
    18121 gagcagcttt ttcacaacct cctacatggt gttcaaaata ttattcgcct tttgcttctt
    18181 tcactaattt tgcgaatgct tgttgttgga attcagcagc taaggctttc gtacgttgtt
    18241 cttgctctgc tttgctcatt tttggatttt cagtcattgc ttttaccata ccgtcttgaa
    18301 cataagcttt tgcttcactt tccatcgtac tttgctgttt cttcactaat tcagctaact
    18361 tggctgcttg gtccgtactt aattctaccc atgtctctgg gattttgaat gtattcactc
    18421 ggctgtcata ttcgtgattg ttatcagcaa tattgaacca gaatttatac atgtcgtttt
    18481 tgtattccca ataagtagtt ttcgtcactt tttcagcatc gccgtctgtc ttttctactt
    18541 tatttgtttc gttatccgta ccagttgctt ttggtttttc ttgtttttca tttgttcggt
    18601 aaagatacac tttttctttt ccattcccta aagctttata caaaagcata tctgctcctt
    18661 gggaatcagc actggaaaca attttttctg tttttgtttc cgttactttt tccattccaa
    18721 agtgattgga caaattagcg acgatcaaac ttagtgaagc gacgaagatt agtccgaaaa
    18781 tcaaagataa aaaggtttgc catgtttttt tagcgaacac gttcgttaaa gcaaaagcaa
    18841 gcaagctgac aatcaaaact ccaataatca tgcattatcc cccgctttca ttagaacagg
    18901 ttcttttttc ttcaataaaa aggcaataat aaagccaaga atcccaaaga tgatcgctac
    18961 gataaacgcg gcatggtaac ctgacagtgt ggcattgatt gcctgatctt tataagctaa
    19021 tggcgtatta tccaatacag atttgcttgg gatatttgct tttgtcacat tcgtcaacac
    19081 actgatcaaa atagctgtac caatcgagct tgcgatttga cggaatgtat tatttaccgc
    19141 tgtcccatga ctgatcaagt tatttggcaa tgcattcata ccagaagtcg ttaccggcat
    19201 catgaccatg gagatcccaa acatacggat cgcataaagg atcacgatat ataaaactgg
    19261 agtcgttttt gtcaagaaag caaatggtgc agttgccgct gtcaaaatga acatccctgt
    19321 gatggctaaa cgtttcgcgc cgtatttatc gaaaatcgca cccgtcacag gcatcatagc
    19381 ccccatgatc aatgcacccg gcaacaacat caagccagaa tggaatgctg attctccacg
    19441 aatattttga atatacaatg gtacgaccat ttctgcccct accatcgcca tatttgttac
    19501 accagctaaa acagctgcaa tcgtgaatgt agatgatttg aatacacgaa gttccaagaa
    19561 tggatgttcc aaatgtaatt gacgccatga gaacaaggcg atgaaaatca caccaacaac
    19621 taagaaacca attactttca agcttcccca gccgtcatct cctacactag aaaatccgta
    19681 aagtaaacta ccgaatccaa tcgttgataa aatagcggat aaaatatcaa tgctaggatt
    19741 tgacaatgga ataacacttt tcattaggaa gaatgctaaa accaagacaa gaacaacgat
    19801 tggcagtacc ataccaaaca agtcacgcca atggtagtta tcaatgatat aaccagataa
    19861 tgtagggcct aaagcaggag caagtccaat aacgatccct gacatcccca ttgctgatcc
    19921 tcttttttct ggcgggaaaa tagatagcat gatattttgc aataatggca tcgatactcc
    19981 cacaccgcaa gcttggatca aacgaccgat tagtaatgta ctaaaattag gtgctgtaaa
    20041 gcaagtaatc gttccgatca aaaagatcgt catggcagaa agatataaat ttcttgaact
    20101 gaatttattg atcagccatg ctgttattgg gatcatgatc ccgttgacta gcaagaaacc
    20161 agtcgttaac cattgcacat cagacgctga aatatcgaaa gctttcatca atgtaggaaa
    20221 agctgtcgtc aataaggtct gattcaaaac ggtacaaaat gtaccaatta ataagacaac
    20281 aactaaaagt gtccgattat atggttttcc gtaaatatct acgggttggt tgttactcat
    20341 atttctcctt ctttctgaat tttttctgaa actactagaa tactttagtc ctaaattcgc
    20401 tatttgtcaa cctacttgac acataagtta atacggatat aattaaggga agcaagagga
    20461 caaaaggagt gacaaaaatg gttggggctt cccttaaacg tttgttggaa aatgatcaat
    20521 tggttgttgg catcagtgaa ttaagtgata tcgtaggtgt gtctccacgt caattaaggt
    20581 attgggaaca aaagggattc attcgttcga tcgaagctga tgcgaattgt cctagaaaat
    20641 atcgtttacc tacagtcata aaagtagaac tgatcaaaaa atatttagat gacggattta
    20701 ctttgtcaaa agctgcagaa aaggcagaaa tccgccaaaa gaaaatgcac catgtccgta
    20761 aagttttttc aaaagcaatc aaagatatcg aattgatcga cgaacgctat acgattttac
    20821 aaatcggtaa cttcaaagaa gagaatgcta agatgtacat cattcatgac aacgagacag
    20881 acgaattaac ttacaagctc cttcctgtcg atgaaaaagc tgactacgaa cagttgttaa
    20941 aagaactcta aacgccacta atctcctaag taccaagctt tttaataagt agatgctaca
    21001 taaatacttg aaaaaaggcc tgtttgaaca taagaatatc cgaacggtga tctgcgtttc
    21061 tttcatatag aaaggaattc tccatcactg ttcggatatt tttttatttt tttcggttac
    21121 tttcttctaa tacatctgtt agccaatttg catattctgt ttcccgattg atcgcatgcg
    21181 ctaaaatcag gtagtgtccg taattttcat gtttttcttt ttcagtagga aagatcgttt
    21241 tttttcgctc taatagatga agtcgttttt cttcatgaag gtcacgctgc tgctgaataa
    21301 tttctactaa tgccggatca ttcacgtctt tgacgaaata caatttcaga acaaattcgt
    21361 ctcgattaac cggtagttca ttggtgggtg tatgaatcca ctctgccaat tgttccttcc
    21421 cttcttctgt taattcataa actttctttt ccaatttttc acctgtgatc tctacttgat
    21481 gctgaatgat tccttgctct tccatttttg caagttcagg ataaatctga ctatgttttg
    21541 cttgccaaaa ttctcctatt tcatttttga acgattgatt caattcatat cctgtcatct
    21601 tcttcttact taataaccct aacaaaatat atcgaagtgt attttttctt gccatcagtt
    21661 ttccctcctt gacaaatctt taaatccatt ctatcataaa tgagtaatcg aaaacatgta
    21721 gtttattaca tgttttaatt tataaggaga tgcttaaaca tgaaagaatt taaagaattg
    21781 gaagatttta ttggaactca ctttatctat acgtatgaca atggttggga atatgaatgg
    21841 tatgccaaaa atgaccatac tgttgattac cgtatccatg gcggaatggt caaaggccgt
    21901 tgggtaaaag accaagaagc tgatatcgtt aaattgacgg acggcgtatt caaaatcact
    21961 tggacagagc caacagggac agacgtggca ttggacttta tgccaaatga aaagaaactt
    22021 cacggaatga tctttttccc taaatgggtt gaagaacatc cagaaatcac tgttactttc
    22081 caaaacgaac atatcgcaga aatggaagca gctcgtgaaa aatatgaaac ttatccaaaa
    22141 ttacttgttc ctgaatttgc tacgatcact tatatgggtg atgcaggtat agacaatgaa
    22201 gatgttatta gcgaagaacc atacgatgga atgccagatg atatccgtag cggaaaatac
    22261 ttcgatgaaa attataaacg agtaaaataa ggttgtgaaa gagaggttgt gaaatcagcg
    22321 ttctgacctg agtattagag aaagaaacgg aaaaatagtt cctcatgttt tgccgttttt
    22381 tgagctaata ccgagggtca gctgattgaa caccgtttat caagaggttg tgaaatcagc
    22441 gttatgcttt gagtaataag aacgctagtt agaaaatagc ttttcatatt ttctaggcta
    22501 gtgggcttat taccgagaag cagccgtttg aacaccgttt atcaagaggt tgtgaaatca
    22561 gcgttttgac ctgagtatta gagaaacaaa tggaaaaata gttcctcata ttttgccgtt
    22621 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaaagat tgtaacaact
    22681 gcatattcac acagttaaat tagtgacttc aaaatgaaat aatgctccca gcaaaaaatt
    22741 aggctgggag cattatttcg ttattctgtt catcaaaaat cagcgttttg attccatttc
    22801 atcacaagtt cctcctcatt tactgcttca tctttttgct gtgtttgaat tgtaaaacct
    22861 tgtgaaagat agaaatgaat agctgattga tttttttgat agactgtcaa agtcagtgtt
    22921 ttattggttt cttttatttt tttcattagt tgcgtaccga ttctttgttg ccggtattcc
    22981 cttttcacaa agattcctgc gataaactga tcaatcaccc cagcaaaacc aataatttct
    23041 tgatctattt ctgctaagta aatcactgca tctggtaaag ctttcctaac agattctact
    23101 tgattctccc aataagttga tggaatgaat tcatgagaat ccaagttagc atgcagccaa
    23161 atcgatagaa cagattccag ttctttggca gacaattttg ttgttgtgcg gatcattttt
    23221 tctctcctta cttattcatt gattttaaaa taaaaaccat ttttttgaag ctcttttaca
    23281 agttttcgcc cctcaaaaaa gtatatcttc tcttgtttta cttttctaat accaaaagaa
    23341 acagcaactt taggatcatc agctgaaacg attcgataag ttccaccagg caattgatcc
    23401 agcttgactt tcccatgatg gtcgctttgt actgaaaacg gcacaccatt tctagtaagt
    23461 gtttttttct tatgtttcaa aatgaatgaa ctaccattta ccggtttccc tgacgaatca
    23521 ctaataataa atgaaaaatc aagcactttt ttacttaatc tatactgata gattagtcgt
    23581 ccgataaaca ctaacagtat gactacagct attatcgttc ctgcaagaat aaaccattgc
    23641 cgcatggttc tttcctgatt tccttttcct tcttcttttt ttaccgattc gttataagga
    23701 atccgatgtc ctgtcactag caaacgatgc gtgttgatca tatacggcgt acatgtaagc
    23761 aaggtaacta agtcttctcc ttctgaaatc gttagaacag acgtgtcatc tggcagtacc
    23821 acttgtatat ttttaacttg ataagccaat ttctccccca aaacagtcaa aataaacgtg
    23881 tctccgtttt ctaaacgatc taattcggta aataaccgtc gattaggtaa accgctgtga
    23941 gcagagatca ctgaatgcgt cgaatttcca ccaagcggga aagaggttcc ttgcaagacg
    24001 cctgctccat tttcaaggat cctaccattg agccgatcat aaagtgaaat atttaattgg
    24061 atcttaggta tattgatact tccgatcagc cattcctcta attggctgga tgttgtactt
    24121 tcatttctgt tcttttcatt gaaaggatca gtaccaggat tgaatccttg gtcagttaat
    24181 cgttggtttt tctcctccat tttctttcgc tgcacttcaa tttcagctgc ggtccgtttt
    24241 tgtacttgtt cgatacgttt ttgatcaata aaactattta aagaatccac ataaaatgga
    24301 tataaagcga tcagtaaccc tacgacgata catattaata aaaacaaacg aaacaaaagt
    24361 cgacgagact gtttttgctt actctgcttc acatgtgtcc tcccttgcaa gtttttttgt
    24421 tctttctttt attatatggg ttcaggaaaa aaatagaaaa tgaaagccga tgaggttgtg
    24481 aaaaagctgt cctgcatcaa gtaataagta cagcccaaaa aaatagctac tcatattttt
    24541 tgttgggttg ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt
    24601 gtgaaaactg cgttatgctt tgagtaataa gagcgctagt cagaaaatag cttctcatat
    24661 tttctaggct agtgagctta ttaccgagaa gcagctgatt gaacaccgtt taccgagagg
    24721 tcatgaaaaa gctactttgt ttcgagtatt aagaaaaatt ctgtttcaaa acaaggagat
    24781 gcaacaaaac ttatgtcgca tcctctctcc ttagaacttt cacttatgtt tttcttgtaa
    24841 aattcataaa tattttcatg aattttctaa tttcttgcct acctctcgca taggatatat
    24901 gatgaattta tctaaagttt gcaaaggagt tcgacaattg aaaaaatatg gcccattttt
    24961 agcaattttt ttcattgctt tggcatttct catgcctcat ttattcacac ataaactaat
    25021 tttgggtgct gatgccccat tccattacaa tcggttctat gatgccgcgc aacaaattaa
    25081 atatggggat ttccattatt ttccttccct ttatggattc cagcaaagtg gcaggatcgt
    25141 gaatgccgtt tacggcccat ttttcgctta tttacaagga ttgatcgtgt tgcttgcagg
    25201 tagctggttt cattatcaga ttctttcaaa tttcattctt tatcttattt caggattttc
    25261 tatgctgatt ttactgagat atgtcaatgt tcgaaattgg gtaagtgtac cagtagccgt
    25321 tttattcatg agtacgtatt ctattcaata ctggtctatc aaccaaggat tcactagctg
    25381 ggggacttgt ttttatcctt tatgtatgat ccctattgct gatttcgtcc ttcataaaaa
    25441 gttccctgtt atcaaagtcg ctgtctcagt gggacttatg acacagatcc atttactgtc
    25501 ttcgatcatg ttagctttga tgtatatccc tttttatctt tattactttt ttaaccagac
    25561 tgaaaaagga aaagcactgc ttcaactgat taagtcggtc ttgctttatt ttgttctgac
    25621 agcaaatatt tgggcaggat tagctattgt ttacagtggg aataagattc ttcctccctt
    25681 tgttaacaga gaaatgagtc aaaaggcgat agatctggat ggcagttatt ggctccatta
    25741 ccctaaggcc tttcctatat tgatattatc aggttttttg ctttttattt tctttcaaca
    25801 aaagaacacc ttattccaaa aaatcgtgtt gtacacaagt cttgttttct tttttctttc
    25861 tactagcttg attccatgga cgacattgat cgaaaaaaat gtgcctttta ttagtttgat
    25921 ccagtttcca tttcgatttt tcgtgccatt tacgctattg cttctacttt accttgccct
    25981 ttcactgaac cagtggtctg aacaaaagtg gttccgtctt ttaagtatat ttggcattct
    26041 tattttttca gcacagacga tccagaactt ataccagcat ctggaaaagt gggaaaatga
    26101 aacatttgtc agtcgacata cttatctatt cgatacgccg gaagaagcaa ggaaaacatt
    26161 cttcagcaaa gatatgtcta ccagcttgtt cgttttccaa aaaaccacac ctgactatct
    26221 gccaatctat aaggagacaa aagacaataa atacgaccgg tataacgaag aaatactaga
    26281 aaacgaaaca acttttataa aaacacatcc aggcggtctt ttgacaatca agtggaaaaa
    26341 cactggggac aaaactgttc atatccctgt tatcgtctac gatcggactg tgctacagca
    26401 aaatggaaaa acattgactg attatgaagt aacagatatc gggaccccaa tcgtgaagca
    26461 gcaaaaagga atcaacgagc tcacgcttca ttatcaaacg ccaatctatt tctatttcat
    26521 tctttctctt actttgattg gctggtttac cttgctatgt ttatttattt atcatagata
    26581 caagctttta cgtgcttagg caaaagggat aaatacatcc gatcaaaaaa ctctccaaac
    26641 ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat gttatatttt
    26701 ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga ttggactatc
    26761 gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga cgaaaataaa
    26821 atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt ccacagtagc
    26881 tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc aactttctct
    26941 tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga aaaaaagtgg
    27001 tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa agccactttt
    27061 tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat catctatctt
    27121 tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa attgggagac
    27181 ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt cttcatattt
    27241 atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt gttcctttaa
    27301 aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat tctttaccgg
    27361 acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt ggttgatttc
    27421 ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt cttgcaattt
    27481 tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt tcattggttt
    27541 tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca caacctctcc
    27601 agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca ggagctattt
    27661 tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct ctccactaaa
    27721 cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat aggaggagct
    27781 atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctcctta
    27841 cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct tgatcactac
    27901 ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt cttcgttttg
    27961 gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt ggccttcttt
    28021 gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca tatggatcaa
    28081 aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga aaatacttgt
    28141 cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag caaagccgtc
    28201 acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa tcacttctcc
    28261 agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac ctgcttgtcc
    28321 atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca gcagatcttc
    28381 gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc cattgtcttt
    28441 gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta ctttttctcg
    28501 atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga tattttcttt
    28561 tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg ttccgctgtt
    28621 agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg gtgtagcaaa
    28681 gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa aagcgatcac
    28741 acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta acggtgtccg
    28801 agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat ctgccatcgc
    28861 aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag caaacataaa
    28921 catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg ctgaaaaata
    28981 cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg caaaagccat
    29041 tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag ccggcgtcca
    29101 gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat tcactaaatc
    29161 agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg cctgtgcgcc
    29221 tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg tcagcatatg
    29281 atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg gtgctagaat
    29341 cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc cagcctgtac
    29401 gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga taacaacaaa
    29461 cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc tataattata
    29521 gtatttatta taagccattg ccccgacaaa tccagcgatg atccctacga atactcccat
    29581 attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga tcttcgtacc
    29641 gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga caccaaagat
    29701 agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg ctccgcctgc
    29761 tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa gattaccaat
    29821 aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg tccaatcagg
    29881 attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg gcataactgc
    29941 taccacaacc attaaagcct ttccaaattt ttgccaaaat tcaaaactaa atatcttttt
    30001 cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc gtgcgttttc
    30061 ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa aacctataat
    30121 acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct tttttctatg
    30181 aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg aacgtataaa
    30241 acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta ttgatccatg
    30301 gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat ctttaacaag
    30361 tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact caggggacca
    30421 ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg tcggctggtg
    30481 gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact ttatcgcgat
    30541 agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag aagactttta
    30601 ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca ctacttctac
    30661 taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag aatcagctta
    30721 tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg tatcaaaatt
    30781 ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga atgagattga
    30841 tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa acaactttgg
    30901 gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa aatcagtcat
    30961 ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag aattatctca
    31021 aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag atattccaga
    31081 agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca attatatcca
    31141 agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg atgtggtcat
    31201 cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac tattctctac
    31261 ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag gagaaaaata
    31321 cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc ttgctttagc
    31381 caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc cgcaagcaat
    31441 ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca ccttcactgg
    31501 tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa atggcgcgat
    31561 tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt tgaaaaatga
    31621 aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc atttttcaaa
    31681 acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg agaacaatat
    31741 caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca cgcgagaaaa
    31801 ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac tagccaaatg
    31861 gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa tctttgtcca
    31921 gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca caagcgatct
    31981 tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca tcaagcaggc
    32041 agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag aaaagcaaaa
    32101 aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc caagtatcca
    32161 ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt accagcgaac
    32221 agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc acatcacttc
    32281 catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa ctgcccatga
    32341 tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt tccatatcaa
    32401 ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt tcgagttatt
    32461 agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag cggatcgtct
    32521 agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt gatttagacg
    32581 gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt ggtcaggaaa
    32641 tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc cgagaagact
    32701 cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag gaagaatttg
    32761 cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg atcgaaccaa
    32821 aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa aaaatcaaga
    32881 ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg ggtcttacac
    32941 cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct gcgccagata
    33001 tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc gggatcgaag
    33061 acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt ggcgtgggac
    33121 gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt cttacgatcg
    33181 attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt cgtaccagtt
    33241 ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt cgttcttctc
    33301 aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa atcagaaaat
    33361 gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt ttttggagca
    33421 actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca tagattggag
    33481 aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt ccaattttgg
    33541 gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt aaaagatgaa
    33601 ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac aaaaaacgaa
    33661 ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc gaccaaccat
    33721 gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca gttaacggtc
    33781 tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa ttggacaaaa
    33841 aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat ccttgcatgt
    33901 tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc caaaacttcc
    33961 ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc taataaaagt
    34021 atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg aaaacagatg
    34081 cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt tcatttcccc
    34141 aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac gatttatcgg
    34201 ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt aaaagatgtg
    34261 gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga tcatcctagt
    34321 atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg ttatgttcct
    34381 aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt aatcttaccc
    34441 cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat ggaagcaatc
    34501 aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa aaattttgaa
    34561 ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg ttttattcta
    34621 gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca catccctttc
    34681 actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga taatgataac
    34741 cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa gaatatcctg
    34801 tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg ttatcaaaaa
    34861 gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca tccggaggat
    34921 tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt ggtcattgac
    34981 gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt cattccagag
    35041 acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca cccttacctt
    35101 acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa gctgcgcgaa
    35161 ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg cttgatcaaa
    35221 agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact aaatacgctg
    35281 accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta acacattgtt
    35341 tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
    35401 cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
    35461 taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt ctgtcccagc
    35521 ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa ccgcctaata
    35581 ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc aacgcgctaa
    35641 ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga gaattgacgg
    35701 tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca acgtcatcaa
    35761 aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct ccaacgatca
    35821 gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt cggtccatct
    35881 tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt aacccaccta
    35941 atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc ccgcctcgag
    36001 aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc atcaagatgt
    36061 ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg agagcagctg
    36121 tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc ccaaataaaa
    36181 taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg ttttggatca
    36241 tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc gtataaaaaa
    36301 tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata tgttcgaata
    36361 gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc gagcagttat
    36421 tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg aatccaagta
    36481 aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat gaactgccga
    36541 cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa cgcacagaaa
    36601 caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc catgaagcaa
    36661 ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg attcctttga
    36721 tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa aacaacaaac
    36781 acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg atcagtactc
    36841 ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa atatttttat
    36901 tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaact attcacttgg
    36961 caggacctcc tactaaatca gcgacaaaac gtcggctgaa agacttattt ttttattttt
    37021 cttctaaact tttttctacc tcaatccccc aagaatcttt cttgaagtga ttgatgatca
    37081 atggaataac gaacatgacc actgtgatag ctgctactaa taatgcatca ctcaatgtcg
    37141 ctgtacctaa tgtagcaaat acgacaacga tgatagagaa gatcagcatg cagctgacta
    37201 cccaagctaa tccgtttccg tttttaccaa ttcggtaagg acgttcgaat tctggatgtt
    37261 tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta cacgatacaa taaataacag
    37321 ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc atccattaca ccataaagca
    37381 aagcaaataa tgaaataacg attgattgtg ttagaacgac atttctagat acgccgtatt
    37441 tgttttcttt atggaatccg aattttggtg gaaggaatcc ttcacgagct acttggatga
    37501 ttgtttttga tggtccagta acccaagctg ataattgtaa aagcacacca ataaatacca
    37561 tgaagctgaa gatatttccg atcacttctg gtaagcctag gatttgacag tcaatcaaga
    37621 taggctgcgt gatatttgct aattccattt ttccatctgg tacgacattt gctacaagca
    37681 tcgcattgat gacattcaat aaaactaatc cgatcaaagc gataaatacg ccttttgtat
    37741 aattttttgt tgcgtctttc aaacgaggca tgtaaacaga actcatttcg atcccaacaa
    37801 aaataaatgt gataccagct aagtatttca atgaatcaat atgttccaag tcagggaaag
    37861 cttttgacca tgaaaagtca cctagatagc cgtttgagac tagtccgact ttgaaggctg
    37921 ccaaaacacc taaaacgaac atgatgacta ctgggatata tacccctagc catacaccga
    37981 tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa gatcgtgatg atccagtaga
    38041 taactaaaat acaacctaaa acgaaccagt gattattgcc aagttctaca ttaccaaatg
    38101 tgtttcctaa caatggtcct aaagtagaag cgaccattac catacctggg aacatttgca
    38161 cccataatag ccatgcaacg acaaatcccc atttgctgcc cagtgctgtt tttacccata
    38221 actgaggacc gccttcttga ggaagcatcg tggaaagctc accggaaacc attgatatcg
    38281 gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat caatgtccaa ccgactgcgg
    38341 ctaacgtagg aatactccga accgttgcac acagtgccat tgtcagaccg ataaaggtcc
    38401 caagacccat tttacttact tcagatttac tcaccgttga cactctcctt gattttttgg
    38461 ataaaataac ggtgtctttg aagtagtttc agcaataaac gaaacaattc agcgaatatt
    38521 tgaggggatt ctttgagttt attgcttgaa gggtttcttc aaagacaacc tcaaaacaaa
    38581 gggatcgaag agcttaggtc tccccttgct cttcagatcc aaacatgcgt cagaaacagt
    38641 tagtattcaa gctgttttct aacatgatta ccttgcttat tttgcttcgc ttgccaataa
    38701 ttggtctgcg tagatttttt ccaatttttc ttgaagagct gctttgattt tttcagcata
    38761 ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt acgctggcac gcaatacagt
    38821 tacttttccg gcacggttcc attcttcatc tgagaatcct agttggttca caaattgtaa
    38881 tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt gaagttaaga actcattacc
    38941 gtaaatgctt cctttcacgt atgaagaata atcatatacg tcatggttca atttattcat
    39001 tgcaactaag tcatcgttgc ctttttcttt gaatacatag tctaccatat tgaaatctgg
    39061 ataagttaat ggatgaactt cgatttcttt gtctcctact ttaaatgaca agtcattcaa
    39121 gaagttgtag aaacgatgtg caccttcgat tgatgcaccc attaatttac catatcctgt
    39181 gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg gctgctgttg cccctgcttt
    39241 tgaaccttcc aagatataag ccccaagtaa tgctgggata tctgcgcctt tttcaaatac
    39301 atatgtcgca aagtaagaga taacgtctct catgcggata tctttgatga cgataccacc
    39361 ggcagaatat ggaacatagc ccattttatg tgggtcaatc gttacagatt ctgcttcttc
    39421 gattgcttta tatgcactgt gtacttcttc taagatataa tctttgtttt ctgtaaagac
    39481 attgtatttg taatgaacat cttttaaatc ttcaaatgga atgaagttgt tgtcttcgtc
    39541 taagaaaatc gcacggccgt atccgccata agcagcatct acgtgtaagt agaagtaaat
    39601 accgtctttt tctaatacgc gacgcaatgc aacgatttta tcgataccgt cgattgctcc
    39661 ttcttctgtt gatcctacaa caccgactac acctaggatt ggtgtttttt cagctgctag
    39721 accacgaacg attttttcaa gttcgttgat gtccatgcga tagttgtggt caactggcac
    39781 tggaatcact tggtctaaac cgataccgat gatgtcggca gcttttagcc atgaatagtg
    39841 tttagtttgt ggaaccaacc attttcctaa tttttctaaa tgttttccgc tacgtgctga
    39901 gtgagctttg atttcgtcga ttttttctgg gacgctgtct aacagattca tgatttcttc
    39961 tgttgacaag ttcattaatt cccaatcgct ttttccagca actagttcag gtgtcacttc
    40021 tttcattgca agcggcaatg atttgatgtt gcgggcatac caaagtcctt ctaagtttgc
    40081 taaagaacca tcagcaacga tgtggcccca gccatcttta tagctcatta attttgcaaa
    40141 ttccattcct acttcttctt ccatttggct tgttgcagga gaagattcat aggcaacgtt
    40201 attaccattc catagcatag caaaattgta cgctagtaaa gatggcatca atgtttcaga
    40261 gttcatatga ccccagtaac gtccggcatt atgccaagga acggaatggg tacgcatccg
    40321 cgctgaaatt tctgatagaa catcctttgt tttattcaca gtgtgttcaa aactagcact
    40381 gcttttttct tctggcgtaa taataggcat gtcttgaggc atgtagtttt gacgccagcc
    40441 taaatgttca tccaccaatt cattcaacaa agctttatag atttggccgt tctcagcttt
    40501 gtccccaatg aatagggcat ttaggtttag atctttcgac aatgattcac tcatagtgaa
    40561 accctcctaa aaaaatattt aaattttgtc gagccctaca ttacacctgt tcttctattc
    40621 tgtaaactaa gttttggcct gatttttaat catattttaa tatgtatcaa ttatccgaat
    40681 tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt tacaaatgta aactccctcg
    40741 caaagattgt attaacgaca accagcgttc tattttagga tgggtttcaa aattggaatg
    40801 ttgtaatagt atttcccttt ttcaaggtaa aaacttacta aattctaatc aattactttg
    40861 gccaaaaagt aatttttctt accttttcga ataaccacaa acttaccatc gaattcatcg
    40921 gatggattta tgacaaaatt taaatcggta acgcggtcac cgttaatact tatagctccg
    40981 tttgaaacat cttcacgtgc ttgacgttta gatggttcaa tttttgtgga aacaagcagt
    41041 tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg gcattttacc gaatccttgc
    41101 gcgatttctt ccgcatttag ttctttaata tttccagaga acaatgcttg cgttattttt
    41161 tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg taacttcttc tgccaaacgg
    41221 cgttgtgctt cacgtttctc aggttctgtc tctacttttt tcgctaagtc ttcgatttct
    41281 tcttgagaca agaaagtaaa gaatttcaag tatttcacta catcgcgatc atcttgattc
    41341 aaccagaatt ggtagaactc aaatggtgaa gttttcttcg gatcaagcca aacggcaccg
    41401 cctgcagttt ttccgaattt cgttccgtca gccttcagca tcaatgggat cgttaaaccg
    41461 aacgcttctg cttctggtcc ttctaatttt cggattaggt ctaatccagc tgtgatattt
    41521 ccccattggt ctgctccacc gatttgcaat tgcacatcgt atgttttatg cagatgcaaa
    41581 aagtcgatag attgcaggat ttggtaagta aactctgtaa atgagattcc tacttccaaa
    41641 cggctggcaa cgatatcttt tgctaacatc gtgttgatgt tgaagttttt accgtagtct
    41701 ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt agttgttcac aaatgtgata
    41761 tttgcatctt taccaaacaa ttttctcatt tgattggaaa gtgcgtccac gttatgttgc
    41821 acttgttcca tcgtttgcaa cacacgttct gttttgcggc cgcttgggtc gccgatcgtt
    41881 cctgttccgc cgccaatcag aatatatgga tgatggcctg ccaattggaa tcttttcatc
    41941 atcataaatg gaatcaaatg tccgatatgc atcgaatcac cagttggatc aacgccgcaa
    42001 taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc tttcttcgtt tgtttgctga
    42061 ttaatggcgt cgcgccaagt taattcatca ataatattca ttttactgac ctccgtgtag
    42121 tttttttatg aaagcaggaa cctgctagca tgtgtaatta attatttctt caggatagga
    42181 taggaaataa aaaaagtccc ttggcatata agccaaggga cgaaatctcg cggtaccacc
    42241 caatttgtat gtctagatat ccaacatacc tctcgaacat ttaacgctgt cgcgtggcaa
    42301 tcactgccaa agactcctga aatgtaattc gctttaaagc atgtactggt ttgcagcgac
    42361 caccagcttt ctgaaacatt ggctttaaca actacttcgt ttcgatcttc gtcttgttat
    42421 tattaatatg tgtctatcat agagcgcaac tttttctttg tcaacagaaa aaggatattt
    42481 tttttaaatt cctcattttt tcttttttga atccgctcta attccgaaca ttttattttc
    42541 aattattgtt
//
LOCUS       DAYYNG010000005        42254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000005 DAYYNG010000000
VERSION     DAYYNG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.005"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1675)
                     /locus_tag="ACHBFA_000233"
     CDS             complement(107..1675)
                     /locus_tag="ACHBFA_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM9393760.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1679..2089)
                     /locus_tag="ACHBFA_000234"
     CDS             complement(1679..2089)
                     /locus_tag="ACHBFA_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9393761.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2086..3009)
                     /locus_tag="ACHBFA_000235"
     CDS             complement(2086..3009)
                     /locus_tag="ACHBFA_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM9393762.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(3006..3884)
                     /locus_tag="ACHBFA_000236"
     CDS             complement(3006..3884)
                     /locus_tag="ACHBFA_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM9393763.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3871..5208)
                     /locus_tag="ACHBFA_000237"
     CDS             complement(3871..5208)
                     /locus_tag="ACHBFA_000237"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM9393764.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5211..6314)
                     /locus_tag="ACHBFA_000238"
     CDS             complement(5211..6314)
                     /locus_tag="ACHBFA_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9393765.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6388..7896)
                     /locus_tag="ACHBFA_000239"
     CDS             complement(6388..7896)
                     /locus_tag="ACHBFA_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9393766.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSEIEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7862..8554)
                     /locus_tag="ACHBFA_000240"
     CDS             complement(7862..8554)
                     /locus_tag="ACHBFA_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9393767.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8576..9550)
                     /locus_tag="ACHBFA_000241"
     CDS             complement(8576..9550)
                     /locus_tag="ACHBFA_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9393768.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9850..10722
                     /locus_tag="ACHBFA_000242"
     CDS             9850..10722
                     /locus_tag="ACHBFA_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9393769.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11180..13378)
                     /locus_tag="ACHBFA_000243"
     CDS             complement(11180..13378)
                     /locus_tag="ACHBFA_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9393770.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13610..14602
                     /locus_tag="ACHBFA_000244"
     CDS             13610..14602
                     /locus_tag="ACHBFA_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9393771.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14662..16083
                     /locus_tag="ACHBFA_000245"
     CDS             14662..16083
                     /locus_tag="ACHBFA_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM9393772.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16076..17575
                     /locus_tag="ACHBFA_000246"
     CDS             16076..17575
                     /locus_tag="ACHBFA_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM9393773.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17614..19008
                     /gene="uxaC"
                     /locus_tag="ACHBFA_000247"
     CDS             17614..19008
                     /gene="uxaC"
                     /locus_tag="ACHBFA_000247"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9393774.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19167..19850
                     /locus_tag="ACHBFA_000248"
     CDS             19167..19850
                     /locus_tag="ACHBFA_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM9393775.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19852..20688
                     /locus_tag="ACHBFA_000249"
     CDS             19852..20688
                     /locus_tag="ACHBFA_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9393776.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20699..22453
                     /locus_tag="ACHBFA_000250"
     CDS             20699..22453
                     /locus_tag="ACHBFA_000250"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393777.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22729..23070)
                     /locus_tag="ACHBFA_000251"
     CDS             complement(22729..23070)
                     /locus_tag="ACHBFA_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9393778.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23138..25309)
                     /locus_tag="ACHBFA_000252"
     CDS             complement(23138..25309)
                     /locus_tag="ACHBFA_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9393779.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25520..26377
                     /locus_tag="ACHBFA_000253"
     CDS             25520..26377
                     /locus_tag="ACHBFA_000253"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9393780.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26448..27305)
                     /locus_tag="ACHBFA_000254"
     CDS             complement(26448..27305)
                     /locus_tag="ACHBFA_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9393781.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27290..29992)
                     /locus_tag="ACHBFA_000255"
     CDS             complement(27290..29992)
                     /locus_tag="ACHBFA_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9393782.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(30005..31294)
                     /locus_tag="ACHBFA_000256"
     CDS             complement(30005..31294)
                     /locus_tag="ACHBFA_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9393783.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31444..32490
                     /locus_tag="ACHBFA_000257"
     CDS             31444..32490
                     /locus_tag="ACHBFA_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9393784.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32492..34645
                     /locus_tag="ACHBFA_000258"
     CDS             32492..34645
                     /locus_tag="ACHBFA_000258"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9393785.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35118..36569)
                     /locus_tag="ACHBFA_000259"
     CDS             complement(35118..36569)
                     /locus_tag="ACHBFA_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9393786.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36566..38290)
                     /locus_tag="ACHBFA_000260"
     CDS             complement(36566..38290)
                     /locus_tag="ACHBFA_000260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9393787.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38283..38897)
                     /locus_tag="ACHBFA_000261"
     CDS             complement(38283..38897)
                     /locus_tag="ACHBFA_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9393788.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39242..40699)
                     /locus_tag="ACHBFA_000262"
     CDS             complement(39242..40699)
                     /locus_tag="ACHBFA_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9393789.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40740..41660)
                     /locus_tag="ACHBFA_000263"
     CDS             complement(40740..41660)
                     /locus_tag="ACHBFA_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9393790.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41672..>42254)
                     /locus_tag="ACHBFA_000264"
     CDS             complement(41672..>42254)
                     /locus_tag="ACHBFA_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9393791.1"
                     /translation="TMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWY
                     NEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIP
                     QLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGM
                     TAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
BASE COUNT        12397 a         8649 c         7512 g        13696 t
ORIGIN      
        1 tgatgcataa gattgataac tttttttaca taacaatata tacaaacaaa aaaattcact
       61 aactagtgaa aataagttag tgaacctcat ttattaaaga attaaattac agatgatctt
      121 ttccatcata cttgaaaaac tcaaaatcag cataacttcc tcttttttta tttagatcat
      181 gcactgctat cccgataaaa tttccagtaa accctcctga gagtatacac atgttttttt
      241 gcggacctac tttcttccat tcttcagttg atacgtcttt caaatgaaac tgtgctgtct
      301 ctttctttac agtgactttc aaaaaagtct tcttttctgt gagtggaatc gtccaatctt
      361 ctatttgtgc tactccgttt tctgattcta tcatacgtag gcattttccc ttgtcctcat
      421 tccaagtaac ataacaataa atataattgg attcatttaa gtacagtacc agtccagcca
      481 tctgattaaa attattaggc atgtattcaa atgctgtctc cgcatcaaaa gtaaaatccg
      541 tttgccgaat agctaacaaa tgatgttcga aggtagattg aagagattca ccggaaatca
      601 gacgtaaaaa acctggtcta cattttgtat cacaccaatc agcatttggc aaaatgcgtc
      661 tggcattcca gttcttcttt agctctcctt caaatgtatc cataaactga tgatcttttt
      721 cttgttgtac ctcttcattt gtctcaatca ttatttcttc catcggagtt gtctggctat
      781 ctttaagtct taaccaatcg tcggacgtcc aatacacttc ttgaatagcg gtttctcttc
      841 ccaaaatagc cgcaccgttt aatggtctgg tacaaagata tgtcatgtac catcttcctg
      901 acggggtttg tactaagctc ccatgtcctg tacattgcaa tgtagaagca ggtttgtcac
      961 tagctgtcag cataggaaaa ccaggatcaa gctcgtaagg accagtaata ttctttgctc
     1021 ggcaaactgt aactgaatgt cctcttcctg taccgccttc tgccgtcatc aaataataat
     1081 acccgttacg aaaatacaaa tgtggtgcct ccgttttcgc taattctgta cctgcaaata
     1141 ttttatagat gggaccatca agcttcttct tctctgcatc atattcttgt aatacgatac
     1201 cgctggattt attcggtgtg tccagacgat aatcccatag ttcattgaca agccattttt
     1261 ttcccgtttc aagatcatgg aacaaagaag gatcaaagcc gttactattt aaataaattg
     1321 gttcggacca aggacctgtg atctcagatg cagtgatcaa ataattgtgg acatctttaa
     1381 acggacggcc tgcacttttg acatctgtgt aaacaagata aaacaatcca tcagcataac
     1441 tgatctgtgg cgcccatata cttccatttg taggatttcc ttgcaaagat acttgagtgg
     1501 tcaaaatatc cgttgtatgt gaccagttga ctaaattttt cgactcatat actcggattc
     1561 ctggaagcca ttcaaaagag gagacaataa tgtaataccg ctctttcact cttaatatat
     1621 taggatctgg attgaatcct cttaaaatag gatttttcac tttgatagtc tgcatgattc
     1681 aggctccttt tctcattctt actcgatata aactgaatgc cccagctgcg aaataaaaaa
     1741 atgcaaaagg aaacgcaata aactgagtgc caatcaacaa tatgaatcct gttataaaat
     1801 acaaaccagt ggcagttgtt aagtattttc ctttagcagt caaaaacaat gctactgcta
     1861 aaaaagcaag tgaaataaac aacacaacgg caaacagacc agtcatgtcc tgtaataatc
     1921 caaatgtatt ttctaaagac aaagttgaat gatctacagc aagcgactga taactttttt
     1981 taaactctga cgcagacata ttacttaaag taactgctaa cccgccaaag aaaatcaaag
     2041 atagactgcc accgaccatt cctagcaata gttcccattt tcttttcatt tctgaacccc
     2101 ctgcttttct aaatgtatcc attcaggtct tttgatcgat gtttcataat tgttattacg
     2161 ttcagagaat ttgacagtct gttcattttt cttgtcatcc caattatccc aaccagttgg
     2221 gtgaacatgc ccacctatct tacacgcttc aaataagaca tttgcatacg gacgccacgg
     2281 tcttcctaaa tagacgtttc tagtatcagg ttctgcagtc aaggtacagc tttcaaaacg
     2341 ataccctgtt ttttgatttt tcggcgtaga tgctgctgtg atgtatccag gatttttact
     2401 ggctaagcta tggatatgac actgatgaaa aacggcatct gccccaccaa atatgaaatc
     2461 caccgttcct tcaatccagc aatattcata ggtctgacga caatactcag gattggcaga
     2521 agccggtgta caaaaaggag taccatcttt ttgcaaatca ggcaacggac ccgtgcataa
     2581 ggtatcctga taaccagata gtcgacagtt ttttaaagat acgcgatctc ctgaattgaa
     2641 taaagcaaca gcctgtccta ccttttctcc tggacctgct gtattttcga tgtgaagatt
     2701 ttctaatacg acattcgatc cttctaagaa aaaagtagca cttcggaaag tccctaactg
     2761 gctaccgtca agatactcct gttttgcaaa acgattaccg gagataacta ctgttcctgc
     2821 tcccacaagt ttaaaattgg ataatctgct ttctatatat tcatcatatc ttccttcttt
     2881 tactatcaag acctttggat ggctatccgg caggcctgcc aaataatcga ttccttcttg
     2941 gatagtttca aaatccgtcg gggtatctat agcattcttt ccaattatca gtttgtcttt
     3001 catcctcata aatgctccct tatgaattgt ttccaaatat ttctcatttc ttgtgtttcc
     3061 atatgaccgc ctgttaaaga aaaagaagaa aagttttctg gactacccat tgattgatac
     3121 tttttgtcca gcccttttct cagcttcagt acgccttcgt ccaagcacat cgtatcgttt
     3181 tttccaacta agctaagacg agggcgcgga gcgatttttt cttggattgc taacgttgta
     3241 taagctgtca atagatttgg cacatagtaa taaaacccat gatgatctag gcggcgatgt
     3301 agcaaaagta tttcgatgtc cacctgtgcc gctagatcaa tacaaacttt gacacgagaa
     3361 tccaatgctg tcacccacca gcttaataag cctcccatag aaagaccaat cgttgcgagc
     3421 cgttgagtat ctgtgtcttc ccttgtctcc aaataatcga tcaagctgat cacatcataa
     3481 atacgcattc cccataacgt ttttcctgtt aataaaaatt gtttgaacaa ctcgctttca
     3541 gaaatacctc cgcgttcttc aaatccccaa gcatcaatcg accaaacagc atatcccatt
     3601 cctgtcagta cttctgcaaa agatggagaa gctaagtaag gagcaccatg gatcaattca
     3661 cttttcccga ctgaaaaatc tccgccgtga gaatgcaggt aaaggaccgg cggaaacggt
     3721 ccttcttttt ctggataagc atagagggct ggtactgttt ccaattcatt taagtacaat
     3781 tgatagtatt caagcataaa accgttttgt ttttctcggt ataatcgttt tccactaggg
     3841 gtatgtctgc tagatggagc acctaaaaaa tcattcaact gcataagcaa agtttttacg
     3901 aacaaccagt tcatccatat tttctgattg atttgattgg aatttctcaa tactgatttc
     3961 tttagtattt tctaatagaa atgctggtcc ctcgtgattt tcaatcgaca tctggttaaa
     4021 ggaaacctta cccgcaaagc caatataaaa tccttgtctg ctcatttcct ctattcctgt
     4081 catcatagca ggaacaccgg gaactgcatc ttgggccatc gagacatcga tattgtcaaa
     4141 tgtgatttct tgtatgaatt gttccgctaa tccataaata aaacctgcag aagcatggac
     4201 attccttgcc gtgatattcg aaaaatggat ccttctgaat gctggtgtcc gttcatctat
     4261 cggataagcc tttttttccc atacatacgg ttctttccct cgcggaccac agaaataata
     4321 aagattcagt ataaatggac acatcacatt atccatgact atattggata ctcgaatatc
     4381 ttcaacgatc ccgcctcgac ctcgtctaga tttcaatcgt attcctcgat cagtttcttg
     4441 gaagatacaa tttgaaatgg taatattacg aatactacca ctcatttcac ttcctaatac
     4501 gacaccgcca tgtccatgga ccatcgtgca atttgtgatc gtgatatttt cgcaagcgat
     4561 cctttcgtac gtatcttcag tccctgcctt gattgcaata cagtcatctc ccacgtcaat
     4621 atggcaatta ctaatacgca catttttgca tgattctgga tcaattccgt ctgtatttgg
     4681 tgaatccgct gggttcaaga tcgtcaagtt atcaaacgtt gcattactgc aaagaatcgg
     4741 gttgatcgtc caacttggtg actggatcaa tttaatatct ttcacagtaa ttcgatgaca
     4801 gttatggaaa ctcatcaatt taggacgagg ataagcgaga ttatctggtt catttctaaa
     4861 agtatgccac catttttttc catttccatc taatgtaccg aaaccagtga cagagatgtt
     4921 ctctacattt tgagcataga tacaggaagc ataaactttt cgatggactc cttcccaacg
     4981 tgaatgaaca acaggataat cttttggatc gtcagagaat ttcagaaccg ctcctgcgct
     5041 taaatgaagt tcgacattgc tttttaaaaa taaagctcct gttagaaatt cacctgctgg
     5101 aacaactacg gtcccgcctc cgtcacttgc tgcagcatca atggcttgtt gtattgcttc
     5161 agtattcaat tcatcaattg atgcgccaaa tgtcaaaata tcatacatat ttataatgcc
     5221 tccacttcaa tcaatgcttg caagaatgca ccaacgcctt tttgatcatt acaaataatt
     5281 ggttcactga tgtagtaagc atatgaacca tctcgctgat ctgctccgcc taaccctgct
     5341 acttgacagt ttttattcaa gttcagccag ccttcttttg tttccagaac aaattcatca
     5401 agtaatcctt gatgtgattt cttcacttgt ggaagccatt cttctttgtt cagcagtccc
     5461 aaacgtattc ctttagccat tgcgtaaaca aacatactac tgccggaagc ttccagataa
     5521 tttcctttcc gttttgtttc tgttgttact tgataccatg tcccgctgtt ttcttcttga
     5581 tatggaagta atgcagccag tgtcttttct agaatttctt tcagcaaaga acaatctgtt
     5641 tcttcatgaa gtaattcata ggtatctgct gcagccatca aataccagcc aatcgatcgg
     5701 ctccaaaaat gatgggatag acctgttttt ggatcagccc atggttggac cttcttttca
     5761 tcccatgcat gaatcagcaa atttgttttt ggatcgaata gatgtttata actgatttca
     5821 aactgtctca aaacatcttg gtaatctttt tcttcactaa aattctggat aaattcagca
     5881 taaaaaggag aacccatata taagccatcc aaccaaattt gataaggata aatttcttta
     5941 tgccaaaaag ctccttcgga tgtgcgtgga tggtttgcca actgctggga aaggagttct
     6001 gctgcttttt tatatttttc ttcgcctgtt tctttgtaca ataaaaagaa aagctttcct
     6061 gtatttacat ggtcgatatt gtattcttca acgctgtagc cacggatcgt tccatcttct
     6121 tgcacgaaat agtcgatatt gtcttttatg aaatcaaaat aaattttttc ttgggttcgt
     6181 tcccaaagta attggaaccc tttaagtacg acgccatagt cataagacca ttttccttta
     6241 taccactttt cttcatataa gcgaggtgtt ctttccatga tactttttgc taatttcttt
     6301 gaccagtttt ccattatttg ctctccttta aaaattaaaa taggatttgg gacaaatacc
     6361 aagaagtatt tgtcccaatc gtctatatta ctgtgccgct gctttgtatg cttcctcgta
     6421 ctcttgtgtg attcgatcgc ctccttgtgc tttccagtct tctacagctt ttttgaatcc
     6481 atcttcgtca agctgtccca tgatgtattg caccgtagca tcaatgacga ttttttcaat
     6541 ttctgatcct acttcattgt tcgtcggtga atccaatggc acagaaggat ccattacagc
     6601 aaattcttca ttttctttga tcaattgatt agagaattct ttttcagggt ctgtatcttt
     6661 gaaagagaac gtcacttcac taggacgaga gcttgataac ggttgcacct cttgttgcca
     6721 aagatccata tcgataaatt cgatagaacc atcatcattc aattcataat gcgttccttc
     6781 gataccgcca gtcatcaatt tatacacatc ttcttctaaa agatcatcaa caaattgaag
     6841 taatttcttc aattgtgctt cgtcttttac ttgagatttg ggaaatgcta gcaagccgcc
     6901 aataccattt ccttctgacc aaacatgata accgccgcct tcaccatctg agatcttgtt
     6961 tacaggcact aattccatat catcttgaat accttgaccc atcgtcttca gattcttgat
     7021 atccaccatt ccagtgtaga ttccagttct tccttgtgca aattgttctt gctgatcagt
     7081 cttttgggta actgcaaagt cttgtgccaa atagccattc tcaaatagat ctcttgaaaa
     7141 attcaaagct ttcatatact cttctgtatc aaattctgga gtgaatttgc cattatcgtc
     7201 tacagcccag ccatttggtg ctccaaaata agaagtgaaa agtttaaagc tggtataacg
     7261 aatatcacta ggcgcacgat cgccaaaacc aactgtgtcg tccacaccat ttccatcagg
     7321 atcgttttct gtaaattgtt tagctacttc ataaagctca tccatggtag ttggaacatc
     7381 tagtcctaga ttatccaacc agtcttttcg aatgactaag ccagcacgag cgtagtcttt
     7441 ttgtatcggt acgccgtaca aggcaccgtc gatactcgca gcttcgattc tatcttcaga
     7501 aatttgactt aaatttttat agtcttttaa atattctgat acatcccaga atacgcctga
     7561 ttttaacgca ttgcggacag aagagttttt caacatcgca atttgaagtg taacgatatc
     7621 tcctagttca tcagaagcca atgcagttgt taatctttct tcttttgaag catcaggaat
     7681 ccagttgaat gtcagttccg tatcggtttt ttcttcgatc aagttcacaa tcgtgtctgt
     7741 aggcggtgaa gcatgatgca aaatatttaa ccaagtgatt ttagcttttc catcttctat
     7801 ctctgaagaa ctgttacttg aactgctgcc gcatgcggca agtgctagaa ttcctgcaaa
     7861 actaagacct aattttacga gcgttgcttt tttcataaat tttctccttc actgttttaa
     7921 taatattttt gttttcttct gttttttctg tatatgagtt cttccattcc aaatggcaaa
     7981 atatagaatt tgctgtccac gtgactgcgg aaaagaagag actgccaccg ataaaggtta
     8041 aaacacctgg cattgtttgg atgatcatca gataaatccc taccatactt aatatcaaca
     8101 ctaatgagta atgaagatgt gagaatccga ataaaaatgc cattttcatt ttgaaaaaat
     8161 atcctttcca atcataatga gccatgattg ggaaaatata gagtgctgag agcacatata
     8221 tgaacgcagc aatatacaaa gcgatcctaa gataccaatt gtgtatgaaa gaaagatcca
     8281 caagtaaaac aatcccgatg gcgccatata tccagctcat cagcaaggtt tcacgatagt
     8341 tttctttaaa atatgtgaag tacgttttcg tcaaaggaaa cgtttctttt gagcgcagcc
     8401 attttctaat cactgcaacc atcgcatacg atgcaggacc gaaagtaaaa actcccaaac
     8461 ctgcgatgac taaaccgccc cacagaaaat tcaaataaac caactgtaaa atacgattta
     8521 gccttccgtt tattttatct aatgactgca gcatatcttt ccctctcttt ctatcttagt
     8581 aaacgccgtc ttctcctagc tttttggcaa tcttattagc taaaattacc ataaacaatc
     8641 ctacgacccc tttaaacaat cccattgctg tactgaaact taactgtcca ttcttcaatc
     8701 cagctgtata aatataggtg tcgaaaattt ctgcaacgct tcgattcaat gaattcaata
     8761 acaagtacat atgctcgaac cctaagtcta atgtctgacc gatttttaga atcagcaagg
     8821 taatgattac tggacgaatt gccggcaatg taacatgcca tgttttgcgt aagcgtcctg
     8881 ctccatccat ttctgcagct tcatacaact gtgtatcgac tgcagttatc gctgaaaggt
     8941 agatgatcgt agaccagccc aactctttcc agatcacttg tccgatatat actgtgcgtg
     9001 tccattcagg ggatgttaga aaactgattt tttcaaatcc cattgttgca aggaattcat
     9061 tcaatacccc tccgtcaaca ttcaaaaata cataagtaat cgaaacaatg attacccaag
     9121 acatgaagtg agggatgtaa atgatcgttt gaatcccgtt tttcaatcgt ttgttcgtta
     9181 cttcatttaa tagtaaagac aaaatgattg gcatcggaaa aaacacgaag atatttaatc
     9241 cgaacaagat caacgtattt cttaataatg taaagaatgt cggttctgta aacaaacgaa
     9301 tgaaatgttt gaaaccgacc caaggacttc ccattattcc taaaaatggt tgataatctt
     9361 gaaaagcaat aacgagtccc cccataggaa gatacctaaa aatcaagaaa taaaggactc
     9421 ccggcagaat catcacatat aaaaatttat tcgttttgat tcctgcaatt attttttttc
     9481 gtcttctgag cttttcttct tttttcgata gcgtcagttg aaatggttct tgtttcttta
     9541 cactttccat aagcccctcc ttagttttgt gaatgtttgc tgcttatgac ttcatgatag
     9601 ataactatct gaattacgta tataatcatt tttaccgtaa gtaaaaacgc ttacatagca
     9661 atgatttttg tgctatacaa aacgataaag tgtatttttt cacaaataaa ctacgcatag
     9721 caaaccttct atttttttct atctgacact tagcatagga aatgattata tacgtaatct
     9781 ttcaccgatg ataaattgaa catatagaaa cagaaacatt taatttattt tgaggaggag
     9841 cctatctata tgaaaaagaa agtctctata ggagaaagaa gttttacact tttcaacaat
     9901 acgtttctaa ttttgctagc tttgatctgt atcgttcctt ttttaaatat catcgctaca
     9961 tcttttgctt ctacacaaga agtcgtcgcg aaaaagttta tcctttttcc tactaccttt
    10021 tctttagacg cgtatcgcta cattctttct acgccgacca tttttagagc acttgctgtt
    10081 tcgattggtg ttacaggtgt cggaacgatc gtcagtatgt gtgcaacttc actgatggcc
    10141 tacggtcttt ccagaaagta tttgtttggt cgaggtttcg tcaactttct tgttgtattt
    10201 tctatgttgt tcagcggggg gatgattcca acgttcttag ttgttcgttc tctagggctg
    10261 gtcaattctt actgggccat gatcctgcct gtggctgtta atgcaatgaa tatgatcatt
    10321 atgagaaact ttttccaagc cttgccggat agtttggaag aatcagcaaa aatggatggt
    10381 tgcacagatt ttggtgtatt tttcaaaatt atgctgccat tagcgttacc gtctattgct
    10441 actatctcgc ttttttatgc tgttacttac tggaacactt atatgactgc tattttgtat
    10501 atcaacgatt catctaaatg gccgatacag attcttttac gccaaatcgt cattgtatca
    10561 agtgggatgc aagcagaaag tagtgccgtt gatgtgattc caccagcaca aacagttaaa
    10621 atggctgtta tcgttattgc aacagtgcct atgctgattg tttatccttt tgttcagaaa
    10681 tattttgtca aaggagcact cgtaggttct gtcaaagggt gattatgtac tgaacgcaaa
    10741 atggtagttg tgtcagaagt ggtcagcttc aagaaataag aaggcattta cgaaaattgc
    10801 ttcttaaatt tttgtgaaat ttcggcttat ttttcgaaga atctactcct ggaacaccgt
    10861 gtatcagagg ttgtgaaaga gacgttcagc cttgagcgat gataaaaact ctcacaaaca
    10921 ttgcgacgag taacccacag gagcaataag aagcgatttt gaaaaatagc ttcccatatt
    10981 tcacaaaatt atgccttatt gtcgatagcc tgtctcttga acgccattta ttcgtaaaaa
    11041 agaactgtga caagactttt gtcacagttc tttttgcgtg cttagccttg aaatttaact
    11101 atccataaat acaaatacta gggttattct aacttctttt attgcttgaa tcagtccaca
    11161 agaaaaacag cctcttgatt cacaaatatt tttttcgata ttctcccggc gtaatcgatt
    11221 ccttcttttt aaagaaacga ataaaatttt gcggatttcg atattgcaat ctttctgcaa
    11281 tctcttttac ggagaggtta gtctctttca accatttttt tgctacctct aaacgatgat
    11341 tttgaacaaa atcaccaaat ttttctcctg tttccttttt aaatacatta cttaaatagt
    11401 tagggttata atgcaaccta tcagcgatca catctaaaga cagatcacga tcatattctg
    11461 tgtagacgat atgcataata ttctccgaca aatttttaaa ttcatgatca gtgattgctt
    11521 gagagcattc tgtgattggc agaactaatt gttctaaaac aagtttttct agttctttgg
    11581 gatgataatt gttcagtgct tttacataaa tttgtttcat gttatcaaag ttttttgaat
    11641 ctactcccaa taactgtcct aattgaacga gttcattgat caagcggacc gaaactactt
    11701 cccgactcaa aggattttta ttttgtgcaa acaatgcatc aatcaattca tgtactaaat
    11761 tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa tcgattttgt tcttctatcg
    11821 gatatctaat aagtgttttt tcatgagcag caggaacgat ttcctgataa aaaataattg
    11881 aacttggacc agtatttaca cgataataca gggcttcttt tgcccgatca accgattgct
    11941 tgctttcatt cagcgtttcg aaccggttac tgatgcctat actgatagtt aaattcaagt
    12001 attcttttac acgtttttga atgagtcggc aatactccat gacttttttg cttgcttctg
    12061 catcatcttt atccattcga taaatagtgg cttgcatttc ttcattcaaa acgatcggca
    12121 gcattcgatt ttcctttggc acaatttccg tgatcatgtt gttgatcgct aaaagtagca
    12181 aatcacgctc cccaccgtga ctatcttcta acagatcgat ttgaaccaaa gctgtataat
    12241 agcagtcatt ttgagaacta taaccaaatt gctgcaaccg ctgatctagt tctcgccttt
    12301 ctactcgatt tctaaataaa ctaagtacaa atagcgtttc cagctgtggc ttttgggtaa
    12361 ctaagtgtgc acttaacgct tctttttctc ctatgatccg actgactgat tgagcgacca
    12421 aatctagttc tttcccactg aatccttttg atttaagatt cagacgttcc tgtatttgag
    12481 aaatcggacg ggcaaatctg tccgaaaata agtaggaaag aagaccaatg agtcctacca
    12541 gtgttaaaga cactgcaact agcccgattc tcagattgcg gatcattgcg ccaattgcac
    12601 tagagtcaac ttctaatctg taaatccaat tattataatc tgatttcagt actatatacg
    12661 ttttatcttt ttctcttatc actttcagct gattgtaatc tagatccacg tccgttaata
    12721 cagttggttg ctctttattt tttactgtct gagtagtaaa aaggttgcca acttcgtttt
    12781 caatagaaag tatgtcttct ccactttcac caacaacttg ttctattgta tgtttctcta
    12841 tcgtagcaat tcccaatgca tatttttctt tttcaaacat tggcaaagaa atcaccattt
    12901 cgattccttt gtttttcttt tgccaaaata aattattttt gttatttatg tacttctgtt
    12961 gatacttttc aatttcctct tcggtcagtt gactcaacga tccattgatt actccccatt
    13021 taccagccaa actgactaaa tcataattat tatcatccat aacaatcgtt tctataaaat
    13081 ttagttcttt cctgatttca gaataagtgg agtaatctgt atatgtcagc ggatgcttga
    13141 atatgtccga gaaatttttg gtcgaaccat acgtagaaaa cgaatattca atcgttctaa
    13201 ttttccattc aacgttagtt ttaacttgcc tgagcaaatt ctttttgtct gttaaataac
    13261 tcgattcgat cgattcatag gtattctggt aaatgaataa actaaatcct atgacaagta
    13321 cggctcctaa taaaaacatc ggtaaaaata ttttataaaa tactgtactt ttcttcattt
    13381 tcttcctctc ctccttttaa aataaaaggc tttcattttt ctgtaaaatt gatactcatc
    13441 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
    13501 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
    13561 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
    13621 caaagatgtc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
    13681 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
    13741 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
    13801 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
    13861 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
    13921 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
    13981 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
    14041 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
    14101 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
    14161 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
    14221 catccctgtc aatccagcct attttttaac aggagattac agcttagaaa gcggcctcca
    14281 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
    14341 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
    14401 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
    14461 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
    14521 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
    14581 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
    14641 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
    14701 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
    14761 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
    14821 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
    14881 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
    14941 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
    15001 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
    15061 acagatactc ccccacagag cctcccgggc aaactgacag cctttttata ccgacgtttc
    15121 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
    15181 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
    15241 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
    15301 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
    15361 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
    15421 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
    15481 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
    15541 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
    15601 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
    15661 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
    15721 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
    15781 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
    15841 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
    15901 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
    15961 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
    16021 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
    16081 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
    16141 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
    16201 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
    16261 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
    16321 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
    16381 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
    16441 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
    16501 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
    16561 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
    16621 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
    16681 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
    16741 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
    16801 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
    16861 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
    16921 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
    16981 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
    17041 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
    17101 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
    17161 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
    17221 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
    17281 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
    17341 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
    17401 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
    17461 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
    17521 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
    17581 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
    17641 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
    17701 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
    17761 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
    17821 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
    17881 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
    17941 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
    18001 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
    18061 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
    18121 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
    18181 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
    18241 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
    18301 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
    18361 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
    18421 gcttatcgta cagaaacact gatacatttg acccacttct atcattctca taattggacg
    18481 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
    18541 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
    18601 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
    18661 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
    18721 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
    18781 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
    18841 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
    18901 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
    18961 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
    19021 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
    19081 agaagaagac ggaccaaatt tgctccgtct tcttctcttg ctgttctgct tcattgccta
    19141 taaaccttta tgaaagaaga gataacatgc ctactatcca tattgccggt gattctactg
    19201 cggcagaaaa agatgaaaac aagcgtcccg agacaggttg gggagaaaaa atctctgctt
    19261 atttttcacc cgagatccag atcaataacc aagcaaagaa tggacgcagc accaaatcat
    19321 ttatccagga aggaaggtta gcccgtctag cttcacagtt tcagccgaat gatttcttat
    19381 ttgtacaatt tggacataac gaccagaaaa tccaagaaga aagaggtacg cttcccttcg
    19441 gtagctattt aaaaaatctg cagatatttt ttgaatgcgc aaaaaaagcg aacgtccaaa
    19501 tggtcttact gacatctgtg acgcgacgag attacttaga aaacggaaca ttgaatccag
    19561 atatacttgg agactatcca aaagcgatgc gtgcatttgc tgagaaacac catattcctc
    19621 ttttagatgt gttctcaaga tcacaagaat tgttccagac cttctcaaaa gaagagacaa
    19681 aaaaattcta cctgcatctt atacctgata cttgtaaaaa ttacccagaa ggactaaaag
    19741 acaataccca ctttagtcca gaaggcgctg ataaagttgc ccgaataatt gttgaattga
    19801 taaaagaatc ccgtctgcct cttgctaatt atttacaaaa aggagtttga gatgtttcca
    19861 ttaaatttag gcatccgtgc ccacgattta gatgttcgaa atcgtgaaga aatcgttcaa
    19921 aaagtatcac agcttcactt gtcccatatc caatttgcac cacaaaaagc atttcctgaa
    19981 caacttaaat taactgacat gacacttggg agtgctgcct attttggtga atacttccaa
    20041 aaaaacaata tcgaactttc cattttagga tgctatatca acctatccag caaaaatcaa
    20101 gaagttcgtg cacaagcatt ggcaactttc aaacagcaaa ttgctctgtg ctctgcctat
    20161 caagccaaga tgattgctac ggaaaccggt agtgtctctg tcggttacac aaaagaaaat
    20221 tttactgaag aagcttatca gatcgccaga aattctatca ttgaattagt cgctcatgca
    20281 gaaaattttg gcgtaactgt tgctgttgaa gcagggatca atcatccaat ctataactat
    20341 cagttagcta aacgtttaat cgatgaagta gcttcaccaa atttaaaaat cattttagat
    20401 tgtgcgaatt tgatgcataa agaaaatcat gctgatcagg aaaaagtgat ccgaggtgcc
    20461 ttggacaatt tacatggtta tattacagcg cttcatctca aagactatat catggaaaat
    20521 ggaaagatcc gcatggtacc tgttggcaag ggctgtttag atttccgccc ggtactgcat
    20581 tatatcaaat atgagcgtcc tctaatgtac gccactcttg aagctactcc tgagatttat
    20641 gttcctgaag caatagaaca cttgcagttt atttaccaag attgttaagg agaagattat
    20701 ggaggaacga atacagaaaa gttatcaaaa atatcatgca aatgcttttc agacattgct
    20761 gcatctttat cagggaaatt attggcgttt tgccatgtca ggactctttt ttattatcaa
    20821 acatcttcca tcttggttaa tgccgatcgc tattgccaat gtaatcaatg cagcttcatc
    20881 gaaagattct tctcagttgc aggccattta tttcaacgct tttttgatgg cttgtctgat
    20941 tggtcaaaat atattgacga attattttca tgtaaaatac catagcactt cgatccgtca
    21001 agtagaagct ggactaagag cgacccttat caagaaaatc caagaactat ctattcctta
    21061 tcaaaaagaa ttgcaatctg gacgaatcca gtcaaagatc atccgtgatg tagaagctat
    21121 ccaaacgttg tcctctcaag tatttgtcag cggattaaat atcgctgtca atttattcgt
    21181 agctttaggt atcacgcttt ataaaagtcc tgttgttttc atgtttttct tattgactgt
    21241 tcctctcgcc gcattcgtta ctctcttttt ccgtaaaagg atcagacagt ccaactcaga
    21301 cttcaggcaa gaaatggaag agacttctgc aaaagtgatc gaagtgatcg aattagcacc
    21361 gattgctcga gctcatgggt tagaaaaaca cgaaacaact cgcttaaata agcattttac
    21421 tcaaatctat gaaaaaggat tccacttaga tatcctgcaa tctgttttcg gatcagtcag
    21481 ctgggctagt tttcagatat ttcagctgat ttgtttgatt ttcactggga tattagcttt
    21541 acaagggcgc atccaacccg gcgatgtcgt gatgtatcag acgtatttca cgacgatcgt
    21601 caattccgtt tctggaatga tcacgctgat tcctacgatt tcaaaaggca tggaatctat
    21661 cacttctatt ggcgaactgt tgattgctgg cgatgtagaa acttatcacg gtaagaaaaa
    21721 aatccaacat ttggatggaa attttcgttt tgaggatgtt tcttttcttt atccggactc
    21781 actcaaaccg attatcgatc atttttctct agaggtgaaa gctggagaaa caattgcttt
    21841 tgttgggcca tctggctctg gaaaatctac actccttaac ttattgattg gatttatcca
    21901 gccgacttca ggaaaaattt ttctagatga tcagcctttg gatgaactgg atatgcgaag
    21961 tgtgcgaaat tatttagccg ttgttcctca aaccactttg ttgttctccg cttcgatcaa
    22021 agagaacatc acttatggct tgaaaaacgt atcaaaagaa cgtttagatg aagtcatcga
    22081 agctgcgcaa ctttcttcac tgattgacca gcttcctgat ggtctggata ccttagtagg
    22141 cgaacatggc aacaagctgt ctggtggcca aaagcaacgt atctccattg cacgagcgtt
    22201 gatacgtcag ccaaaaatca ttttgctaga tgaagcaacc tcagctttag ataaccagtc
    22261 agagaaaaaa atccagcaag ccctgaatta cctgacagaa acacccacta cttttattgt
    22321 ggctcatcgg ctttcaacga tcaaagaggc agataaaatc gttgtgatcg atgaaggaaa
    22381 aatcgttgaa ataggtactt acgaaaaact gttagctcaa aaaggctatt tctaccgctt
    22441 gaaaaatgaa tgacaaaaaa taggagctat gacaagactt ttgtcgtagc tcctatttct
    22501 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatagtc atcaaacatg
    22561 agaagctgtt ttttgaaatt gttccttgta actcagagtt aaacggtagg ctcacgacct
    22621 cttggtaaat ggtgttcaag agacagccta tcggcaataa gaagcaattt tgtcatcgct
    22681 caaggccgaa cgtctctttc acaccctctg ttgtttcata aaatagtttt attcgctgta
    22741 aaggtcgcga actggtgtca tgatgatacg gttcaagtca ttgatgattg tactgaagcc
    22801 ttgctctttt tgcatcaagt cattgattac ttcttctgat tggattttca cagccatttc
    22861 ttgtgctttc attgcatctt cgtcgctgaa ctcttgtcca ctcatttgtt tttcttgtaa
    22921 ttcgatttgc atcgcttgga agtctttgaa cagtttgtgt gcttcttcgt ttgcttgtac
    22981 tttctcgtac gcttctttca acgctttgaa ttctggtaat tcacggatct cacgttcgat
    23041 ttgattcgcg ctatcgtaga tgtttgacat gttatgccct cttttctttt agtagttact
    23101 ctctattgta acatggggtt attcgttttc aaaggggcta ctctccttga tatttccgcc
    23161 agaggtcttt agccgcttca ccaattccct gaacgccttc tttcaccata tcgccgaagt
    23221 ttttcagccc ttcttttgct tgttcaccat attgattcaa gttatcttgc caatttccag
    23281 actctccatt agaatttcct gtgtttccaa cttgatctgc agctaccact tgtcctcctg
    23341 tggcataggc atctgctaca ctaaatcctg tttttgaagt atacggaagg atgccttctg
    23401 ccgctgattt gaagactttc ccgacaacat ttccacttgt cccttccaaa tagtgcagct
    23461 tgctggattc ttcgtaaccc atccatgtcg aaatcactac gtctggcgtg taaccgatca
    23521 tccattggtc atttgctttt gtggcatcga aattagtttc tgtggttcct gttttccctg
    23581 ctatcgtata acctgcagga ttcgcagcag ccgctgttcc atttgaaaat gtaccaagaa
    23641 gcatgctggt catttcatcg gctgtttcct ttgatatgac ctgttttggt tttttgtttg
    23701 tgttatcgac gatcaccgct ccagtcgaat caacgatttt cgtgataaga tgcggcgtat
    23761 acatctgacc atcattggca aatgcactgt aagcacccgc catgatcaat ggagatactc
    23821 ctttttccag tccaccaagg gctaagccat aatacttatc agattttgct aaaggcaggc
    23881 cgaattcttc tgctttatca tatccttttt gtaaaccgat ctcatgcaaa agccatacag
    23941 taggcaaatt caaactttgt gcaacagctt gatacatcgg tacttctcct tggtatgtcc
    24001 cgtcaaaatt atgcgcatcg taataacttt gcggttcgtc tttcaagata ctggaaggct
    24061 tgtatccagc ttccagtgct ggtgtatata cgcttaatgg tttgatcgtt gatcctggtg
    24121 aacgacgcat ctgtgtagca aagctgaatc ctctaaagac atgatctcct cttcgtccta
    24181 ctagtgcttg tacaccacct gtattcggat ctaaagctac tgaagcagat tggaccatgg
    24241 caccatctga agcatttgct gggaataatg cactgttttg ataagtagct tctaattgct
    24301 tttgataatc ctgatctaaa gctgtataga ttttatagcc tttattcatg acatcttctt
    24361 catcaagacc atactcgtta actgcttcat cgatcactgc atcaaaataa taaggataac
    24421 gataaccaga atctgaatta tcgtatgtgt cattaagata cgcgctgata tctgtctggc
    24481 tttctgcttg ttcttgttct tttgtgattt ttccgttttc ttccattaca cttagtactg
    24541 tgtttttccg atcattcgca ttttttgggt gatcgatcgg attataaata ccaggacctt
    24601 tcagcattcc tgccaaagta gcagcctcag acagcgtaac attcgttgca tctactccaa
    24661 aatactttct ggaagcgtct tggacacccc ataccccgtt gccaaaatag gcattgttaa
    24721 gatacatggt caagatttct tctttgctgt acttcttttc gatctcgatt gccaaaaata
    24781 attccttcgc ttttcgatca aacgtttggt ctaatgtcag atacgcattt ttggcaagct
    24841 gctgagtgat cgtacttcct ccgccccctg atgttccgcc agacaagacc attcggactg
    24901 ctgcacgggc aatccctttg atatcaaacc cgtgatgctc gtaaaaattc ctatcttctg
    24961 tcgagattac tgcatcttgg atatagggag aaatcgcgtt taattctaca tatgttcctt
    25021 tttgggcgta cagtgtgcca gcttcttcgt ttttcttatc ataaatgact gtcgattggc
    25081 ttaatcctga ttttaacgtt tcgacatttg cttgttttgc taaaataaag aggtagatac
    25141 ttgtcactaa gacacagatc aaaccaatca gcaaaatcaa tttgttcaca tgatattttt
    25201 tccagatgcg ctttcgccat cgatggaatt gtccgagata tggtctgatc caacgccaaa
    25261 aactgatcaa agcagctttt atcttttcta aaatgctttg aaaatccatt tgcttcttct
    25321 cctgtctatt tgattcaatc gtcttatttt accatagtga aagagtgttt tcttcctgct
    25381 taagttgtag ttcaatatgt ttttttacgt tagtgcaagt agagtaacat aagaatgaac
    25441 agaaaaatgt aagttttttc ttaaatatgt tttaaaaatg gtacaattag tctgcgattg
    25501 cactgaaagg agtacacttg tgaaaatcac gatcacttta cctatgaacc atcctgaaac
    25561 aacaattcgc gacttgcttg aaaaggaatg gttagttcct agaaaggttc gacacttttt
    25621 gcgtatacgt aaaaacgttt ggatcaatca acaacctgct ttgttccatc atgaagtaaa
    25681 gggcaatgac cagatcacat tgttattcga agaaacagat tataattatc aagaagttca
    25741 attaggagat gcgaagcaca tccgtgtttt atatgaagat gagcatttga ttatagtgga
    25801 taagcctgca ggaatgaaga ctcatccaaa tgaaccgaca gaaaacagta ccttactcaa
    25861 tcatttagct gcttatctcg acactaaaca gcaacgacct tacgttgtcc accgcttgga
    25921 caaagaaaca agcggtgccg ttttatttgc aaaaaatcca tttgtcttgc cgatacttgg
    25981 aagaatgttg gaacaaaaaa tgatctatcg gagatatcaa gccatcgtat ggggagaaat
    26041 tgccaaagat atgaccatca ccaataaaat tggacgagat cgccatgatc ggagaaaaag
    26101 ggtcgtagat gataaaaatg gtcagagcgc cttgacacat gtcactgtca acaaagtact
    26161 ttctgaacaa acacaagtct attgtgtttt ggaaaccgga cgtacacacc agatcagagt
    26221 tcatttagct cataagggac atccgatcgt tggagaccct ctctaccaaa atagaacagc
    26281 tgaacgactg atgctccatg cattagaact gcacatgatc catccattta ctcaagaaaa
    26341 aatagtcggt aaagctttac ctggattatg ggaatagaaa taaaaaagaa cagagtctta
    26401 tcagcagata atgataagac tctgttcttt ttgtgtaatt tgtctgctta cttttgatgt
    26461 tcgtgttctg ctaacagcaa actaccgata atcccacttt cattcggcag tccccaacga
    26521 acgatatatt cacttacttg aggtgtttcc atataaccac ccatcaattc aacaaattgc
    26581 tgacggattt tttgaaggag atgatcctga ttcattaccc caccgcctaa aataatcttt
    26641 tcaggtgcta acgtcaacgt atagttgatc agtgcttgag caatataata agcctgaata
    26701 gcccaaacag gatgatcttg cggcagatct tgccctttga tccctgtgcg tgcctcaata
    26761 gacggtcctg ctgctaatcc ttctaaacaa tctttatgat aaggacaagt accttcataa
    26821 tggtcatctg gatggcgttt cacccaaata tgtcccattt caggatgtgc gatccctgaa
    26881 aaaatttctt cattgatcac gacacctgca ccgattcctg ttcccacggt aaggtaaata
    26941 caactatttt ttccttgtgc tgctccttgc ttcaattccc catatgcagc agcattgaca
    27001 tccgttgtcc aagcagcagg aatattgtag cgttccttga ttgtgccaag aaaatcgaat
    27061 tgtgcccacc cttttttggg agttgccaaa acgtatccgt aattatcttt tgttttgtcg
    27121 ataccaatcg ggccgaatga gccaatcccg attcccttca attcataacg atcaaaaaat
    27181 tcaaatacct gatccaatgt ttcttctggt gttgtggtag ggatacttaa tttttctaaa
    27241 atttctccac catctgaaac cgcacacaca aattttgttc cgcctgcttc tattccgcca
    27301 tacattcgtt ttcctcctcg aatccgattg tccgaatctc ataaggttgg attattggga
    27361 caaccgcttg cttcgttggt tcttcaagca gattcaatat gacagttttt ccattgtctt
    27421 tagttatttc caatttttct aaatgactag acatattgaa acctcttact actactttat
    27481 cactaaactt gctgcgtttc aaagctgtaa ttgcaaacgt ttcacttttt atcgttaaat
    27541 attgttgttt tgaaatcaat gttccttcat gatgtttgat ttgttgagtg gaaaaaggaa
    27601 tctgtgctgc gtatgcatgc aaataagtag agaatttttc ttctggtcca tgtagttcaa
    27661 tgctgtaatt gaaccgatgc tcacctagac actgtgcttc tggtgttgga aaatatcccc
    27721 aatcgcctag ttctcccaca ctgcgaagta gcgtcactgc gatttgtccg tcttctgtta
    27781 cttcatattc attcaaacca aagtttccga ctgttacacc gtactcttca tcatgaatat
    27841 tgacaaatgc ttgctgatgt tgcggattcg tggggttttc ccaactttta gacacatggt
    27901 ttgggcgctt caccacttca aaaatactat ctgcttcgtg atgttccaca tgtaactttg
    27961 taggaaacaa gacacgtaag cgatgatctt tcatctgatt gtcgagagtc gtttcaaagt
    28021 cgattttttt actatctttt ctgattgtca tgattgtttt tatttgaagg attcgttttt
    28081 ctttggatcg ttctgctttt cgataacgaa attccacaac catctgctgt tctttttcta
    28141 aacgctcgtc tgcagaaaca gggatttcca tttcctgtag aatacttatt tttgctattt
    28201 ccggtgtatc tactaatact tttacttcag agttttcttt gttactggat aggattggct
    28261 gatcacagaa aggttgttta aagatatact catttcctat atcgccgata tcttcaaaaa
    28321 tatgcagatt attcaacttt cgtttgtttt tgcgatcttc cattgtaatg gtaccatttt
    28381 tttcaatcgt caaatgtaga gggccgtttt cgatgattcg tccactctga tgaatcattg
    28441 tttccttttc ttctgctttc gcttcccctt ctacaagcgc gaatgtttcc caagaaaatg
    28501 ctggcatgtt ttctaatggt aaagtcactt tgatcattct tgccatatat ggcacacgga
    28561 aacggtcttt cggcaaatca taaccaaatt ggacagtttc ttcagataaa atagctggta
    28621 tagtggcccc actttttgtt tctacatgat acttacgttt aggcaaattt tctaactctt
    28681 gatatagttg ttcaggtatt ccttcagcga atctttttct ttctaattcg atggttattt
    28741 ctgcttcacc agttttatcc attcctgcag tatttacaat gacaaatgga aagctgtctt
    28801 ttgggaatcc ggtcgtatcg attgctgcgg tcagctgttc taaggaatct tgtgcaagat
    28861 actttccaac ttcatctgct ttttcaaaac gaggtatcat ttcccgatgg acagaatcga
    28921 ctgaacaacc gcaaatactg tcgtgaggat gattttgcat taatgtcttc caagcataat
    28981 ctaattgatc atggggatag tttccactaa cttcataagc catcgtcgca agtggttctg
    29041 taatattttc tagctgtcgc tggaccttgg tattccattg ttttaagtaa acccgagcag
    29101 atgctgtatt ggctaaagta taccagccat ccgtttcttg acttgtcaat tctccttcta
    29161 ctgagcctaa gtcttctggc acatcttttt gaactgcttc cagatagtca gtaaaattag
    29221 aatgaataaa ctcgtaatct ggaaacagtt catttgcgag atggattgct tttgatatat
    29281 ctttttgtac cggttgatga tcaacaccat tcatcatcaa taagtggttc gtagaggcat
    29341 attgttctgc atccgctaat ttttgcttcc aaaaggcaag agctgcttct ttttctactg
    29401 gaatctcatt cccgttgcta taccaattgg caaatagcag accaaagatt gctgtttgat
    29461 caggtccttt ccaccacatc tctgagtatt gggaagaata gttttctgct tccaaaactt
    29521 gattatcaaa gccaattggt tttacaccac ggccaaacgc tgctgctgaa ataccagctt
    29581 gtttcattaa ttgaggcgtc tgtcccatat ttccaaatgt atcgggaaaa tagcctaaca
    29641 tgacaggggt tccccattta cggctttctt ccatgccaat cagcatattt ctcacgttcg
    29701 attccgagct gataagaaaa tcatcttgca agatataaaa tggtccaatt ctcaattttc
    29761 cttcattgat tgcttgttgg attgcctgtc ttttttctgg ccttacttga agatagtcat
    29821 ctaaaataat cgtttgccca tccaaatgaa aactgttgaa ggacggatca acttcaaata
    29881 gctctagtag atcgtctatc aattcaatca gtcgcatgtg atgctgttcg taagccatgt
    29941 accattcacg atcccagtgg ctatgagaaa tgatatatac tttctttttc atttacgata
    30001 cctcctactt ttctactcta atatcaaagt aatccatgac taattcgcaa aacatcatat
    30061 ttgcccaaga aaaccattct ctcgtataat tttcaggatt gttcacatcg aatccttcgt
    30121 gcataaggtg tgttcctgcg tcagttgcta caagtaaatt caatatccgc tctttttcag
    30181 atttgtctga cgttgtcatt ccttccatag ctaatgcaat cggccaaacg tatttttctg
    30241 gcgtatggga ggatccaatt ccttttgcgt actttccttc atagaaatat ggattttctt
    30301 tactcaacaa tgttcttctt gtttgcagat aatcttcctc ttgttcagaa caatatccca
    30361 agtaaggtgc ggcgatcaga ttgggcacat tactgtcatc catgactgtg gcgtttccca
    30421 ttccatctac ttcatatgca taaatcgttt gccctttttg attaaccgtt cgtccgtgat
    30481 tctgaatccc ctcttggatc tctttcttca atttctgtgt ttgctgtaca atttctttat
    30541 catcgagtat cgtttgaaaa atttcttcca cataacctaa cacgaccacc gcaaacatat
    30601 tggatggtac cagatatcca taacggcaag tatcatcgct aggacggaaa ccagaccatg
    30661 tcattcctgt aaaagctgtt tttgcacctc gcccgtcatt gatcagcgta tcttcttttc
    30721 ttgtcgtgtc tcgtacaaat gtataaggag agtgttcatg atcttgctct gttttgaaca
    30781 cgtccaagat ttttttgata ccttcaataa aagattgatt gaattgatca gtttttcctg
    30841 tatttttgta aagaagatag gccaactgta ttgggtagca taaagaatca atttcatatt
    30901 ttctttccca gatccaatcg ttcatttttg tatgatctgt ttggtggcca gcaccgttag
    30961 cttcttcgtt gaaggcattc gcatatggat cgatattgat ataaaaaaat tgctttttga
    31021 ctaacccgct gatcatgtca gctaattcct catcttcttt tgcaatcact aaataaggac
    31081 gaacttgtgc agtagagtct cttaaccaca tcgctggaat gtctcccgtc aataaaaagg
    31141 tggttccgtc attttgcttt ttgactgttg tcaacagagt attggcaaat gctgctgtaa
    31201 aattctttgc ccagtccgca tggatatccc cacatttttg tgtgatatct tccataaatt
    31261 tctgcaccga ttttggtata tctttataag ccactaataa aaccttcttt ctatttgata
    31321 tatctttaaa gattatactt catacagcca attatgtcta cgctttcaaa aaatatcttt
    31381 ttatttactt tttcatccat tatggtatta tttgaatatg atatatcatt aagaggtgtt
    31441 tatatgaatc aaccattgta ccagacaata tttaatgatt tgcaaacggc tattttaact
    31501 ggtgaattac ctgtagacag ccaactacca actgaaaaag aactttcacg tacctataaa
    31561 gtaagcagaa tcacttccaa acgtgcattg actgaattag agcaattagg acttatttat
    31621 cgcgttcgcg ggaaaggcag tttcgttaaa tccgcttctc cggaacagat tcttgcttct
    31681 cctacaaaaa cacgacggat tctgtttctt cttccatatg tcgctgattt gtctgttggt
    31741 gattttagca agggattgaa ccctgtgatg caagataaac agattgatgt catgatgacg
    31801 acgcttgact ttctacaaaa taaaacagca aaagagctaa tcaatgaatt tgacggattg
    31861 atttattatg ctcttgatac agaacaacat cttgacttac tttttgagct ttcattgaaa
    31921 gaattccctg tcgtcatatt agataaaaaa atctatgagt taccttttcc taccgttctt
    31981 tccgataatt tccaaggcgg tgcgcttgct gcctcttatt tgatcgaaca gggacattca
    32041 aaaatcgctt atttgtttgg tacgcagact catccacaat ccgttcgaca gcgttacctt
    32101 ggttaccttg aagcattgga caaagctggt ttgactttcc acacttcact ggatgacaaa
    32161 agcgcgacat tagatgaatt gctttcttat atcactaagt atgaagtaac tgcttttgtt
    32221 tgtgaaaacg atgttgtcgc tatccagacc atgaatctct tgagacagca tggttatcat
    32281 atccctgaag atttttcaat tatcggattt gatgatatcc aagcagcagc tcttgttgac
    32341 ccgccactga caaccatcgc acaagatttt gaacagttag gtaagcttgc gggagatgct
    32401 ttggtcactt ggttagagac aaaagatcga ccatccgata gtaaatttcc agtttcattc
    32461 atcaaaagac aatcaacaaa ggagaaataa aatgaatatc ggtcaaatag atactagaca
    32521 tgcaacagcg aatcagccaa atttctctaa tgggaactgc cagccttata ctggcgttcc
    32581 ttttgggatg aattattttg caccgcaaac tacggatcaa aacggcagct ggtggtttca
    32641 tcctgatgac cgcgtttttc aaggctaccg tcttacccat caaccaagtc cttggatggg
    32701 tgatttcagt catatgctgt ttacgccgat caatggaaaa ctacaggaga acaccctttt
    32761 ccatgctcaa agttcttacc gcccagaaga aagtatattt tgcccgactc atctgtcgat
    32821 ctgtcaatta cgttatggca tctgttctac attgatacct agcatgtatg gcggaatctt
    32881 atctattgat tacagcaaaa atgacagcgg tttactttta tcctttccgg gacgccatca
    32941 gctctttgtt atagatcctt acacagtgga aggaaaaatc acacattttg ctggttcaga
    33001 agatcctgat ttttcttttt attttatatt gagatttgaa tatcctttaa caattgatga
    33061 gttagctgtt tccaacaaaa atagcgactc gatcccgttg tattttgaac agacaaaaaa
    33121 acaaactatc cgatttggga catcttttat cagctcggaa caagcagctt ataacctgga
    33181 actagaacaa aaccaaagca aagagactta ccttgctcac agtcaagaac aatggcagtc
    33241 ctattttgat cgtatacagc tcacacacca tgatcaacaa caattgcgga cgttttatca
    33301 taatttatat cgggtgtttt tatttccaca aacattttat gaaattgatc ctagtgggca
    33361 aaaaatccac tatgatacga ctagtcgatc tataaaacct ggtgttctct atacaaataa
    33421 tgggttttgg gatacgtata aaacggttta tcctctttat tctttgattg ctgtagaaaa
    33481 atacgaagaa atgctggaag gttttttgaa cagctataga gaaagtggct atttgccaaa
    33541 atggctgtct cctgatgaac gtggacttat gcctgggaca ttgatcgatg cggtcattgc
    33601 agatgctgct gtaaaagata tccgacgaga cttgatgcct gaatttctaa cggcaatgga
    33661 aaaaggtgct acggttcaaa gcaaacatcc aaattatgga cgccaaggaa cagaagatta
    33721 cttgacatat ggctatgttc ctaatcacta ccacgaatca gtcaatcaca cattagacta
    33781 tgcttatagc gatttttgta ttagccaagt tgccaaggta ctagaagaag aggaaatgac
    33841 gaagaaatat cagcagcaat cattgaacta tcaaaatatc tttgactatg agtcaggatt
    33901 tatgcgttca aaagataaag atggcaagtt cagagaacca ttttcagcta atcgttgggg
    33961 acaagattat gcagaaggaa gtgcttggca aagcagcttt gctgtttatc acgatttttc
    34021 aggactgatc ggaaaatatg gtggcagtga gagatataaa gaaaaactca ttgagctgtg
    34081 caatcagccc ccaacgtttg acccaggagg atatggtttt gagatccacg aaatgagtga
    34141 gatggccgcg atagaatttg gacaattagc tatttcaaac cagccaagtt ttcactatcc
    34201 ttacttgttc agttatatag gacaacctga aatggcacag ccactgctta aacagttgat
    34261 gactcagacc ttcgacgatt ctccaatggg ttaccctgga gatgaggaca atggaagtat
    34321 ggccgcttgg tatatcttca atagtctggg attttatcct gtcacgcctg gaaccggtga
    34381 atatgttatc ggtatgccat taattgacaa ggctgttttg cttttgtcaa atggaaagga
    34441 attgacgatt gaagcaacgc ctaatcggcc gcagcaacaa tttatccata aaatcaaacg
    34501 gatggacaaa gaatatacga aattatattt tacccatcaa gatttgctga aaggcggcac
    34561 gatctcttac caattaggga tcatccctca ccctaagaaa tatcaagcaa aagatttgcc
    34621 tttttcctta tcaaactata attaacaaga ggttgtgaaa aagctgtcct gcatcaagta
    34681 ataagaacag ccaagcaaaa atagatcctc atatttttcg tttggttggt cttattaccg
    34741 cagatgcaag cttttgaaca ccgtttattg agaggttgtg aaatcagcgt tctgacctga
    34801 gtattagaga gacaaatggc aaaatagttc ctcatatttt gccatttttt gagctaatac
    34861 cgagggtcag ctgattgaac accgtttagt ggagaggtcg tgaaaaagct gtttagtttc
    34921 gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa
    34981 taccgagaaa ctggcttttg aactccgttt atcgagaggt catgaaaaag ctgccttgcc
    35041 tcaagtaata agaacagccc aaaattagat ttctagacaa ttttttataa gttatttcat
    35101 tacattggct ttttctttta tgattctcca ctgttttctc ggtactcttt aggggatctg
    35161 ccgacgattt tcttgaataa tttagagaaa tacgtggtgt tgttgtagcc aatctcactt
    35221 gctatctcat tgatgttttg attactgtgc aaaagcagtt gtttcgcttt ttccatacgt
    35281 aaatggttca aataagctga aaaacttttt ttcgtttctt ttttgaacag ctgtcctaga
    35341 tacatgacat ttaaatgcag gttttctgac acttctttga gtgtaagtgg ttcttgataa
    35401 tgctggtgca ggattacgat tactttttcc acattgtcac tgtacttttt ctgtctctga
    35461 tgctttttga tgaatgacag aagtagctgc tgaagttctt gcaccgtttt tgcctgatgg
    35521 attttttcga ttccttgtaa atattcctca tcttccagat caatcaattc ccgttgaatc
    35581 tccataaaca gtaaaaaact ggaatatcgt atatcttccg gcatcatggc tgctaactga
    35641 aactgttcaa aaaaatcagt gatcattttt tgagcgactt ccaattgttt attttgcagc
    35701 acgcgtctga aacttgaaaa atcgatggtt tgatcttttg tttctgcttg tactgcgtaa
    35761 aaaacatgct cgtctttatc tccataaaat tgatgcaact gtagattgtc tttcgcttgt
    35821 tggaatgttt cagggatttt gtccaaagaa tctgtctctt ccccgatact gatcagccat
    35881 tcctgatcag gaaagttttc ttgaacaaaa caacagaagc catcaacttc atctgcctcc
    35941 aacaacaacg tgatcaatac acggtctccg taatttcgtt gatagtaaaa gatttgttgc
    36001 cgttcttgca accatctatt gataagagta tcatgagttc ttgccagttg aatcaatact
    36061 atcctatgct gcttctcact tatttgtcct attatttctt cttcacttat ttcatccaat
    36121 tcttcattca accattgact gaataacagt tcttgataga tttcttgttg atttttttgt
    36181 tcattttgct gatctaaccg tgttttgatt ttcttgaggc tttcaaccaa ttcgaactta
    36241 ttcacgggtt tcatcaaata attcacggcg cctaattgca tacctccttt caaataatcg
    36301 aattcttggt atcctgataa aatcataaat tcaaaattat gacgttcttt ttgagccgct
    36361 tcaataaatt ccagtccatt tatttctggc attgtcacat ccgttactac taaatcaatc
    36421 tgtcgctgtt caagtacagc tagtccttct ttggcattct ctgctgttgc tactatttga
    36481 aagcctaaac tttgccaatc aattatcttt tttaacccat tcaaaatcat gtattcatca
    36541 tctaccaata acacttgata cacgctcatc cctccttgaa tgtaatagaa acggtcagtc
    36601 cacctgtctc attattctca agcgacatat aataactgct gccgaagctg gcacgtaaac
    36661 gttcattcac gttttgcaga ccgatagaac cgtgaagttc cactttagga tgagacagtt
    36721 tttcttcaat ctgttttagc cgtttttcag tcatcccttt tccattatct ttgatgatga
    36781 tccttacccg tttgccttcc tgcaagactt tgacacttag cgcattatcg aatcgagtaa
    36841 aatcaatacc gtgtttaaaa taattttcaa ctaatggctg gatcacaaat ttaggtactt
    36901 cgatcttttc caaatcagga tcgatcataa aatgataggc tactcgatta ggataacgca
    36961 tttgatacaa atagacatat ttttcgcaaa agtccagttc ttcttttaaa gtgatcgttt
    37021 tctcttgatt cgtattgttt cgaagcaaag cggaaaacgc atacacaaca tctgctaatt
    37081 cttcacttcc ttcgctgatt gcatacattc ggatatattc taatgtatta tataaaaagt
    37141 gtggattgat ctgtgcttga agcgcccgca tatgtgcgtc ttgctgtttg atttctaatt
    37201 tgtagatgtc ttctacaaac tgattgatgc tatccaacat ttcgttgata ccgattgaaa
    37261 gttccttcag ttcatattcc gtgtttgtta tgtcgatccg cgtctctaaa ttccctgctg
    37321 ctgtttgatc catggcctgc atgacgatag atacttgctg tgaatatcgt ttgaatgttt
    37381 tataaagaaa gataagcagt atattgtcta aaataatgat accaatgatc agcggggcaa
    37441 gattttttcc cgtttcgatt tttacacttt ttttatctag tacagctaat atacgataat
    37501 catcagacag ttcttggtgt tgaacaaaat tgtttttttc cagatcgtca atcggcaatt
    37561 gtgaattttt cttcagactc tctgtgatga tcatttcttg tctttcaaac gtttctttag
    37621 ctattttatt atcatggaat gtgtatagtc gatttgtcgt tccagaaatc atgtataaag
    37681 agagtccatt gaatgacgtt agattttcta aaatcatatc tagttctttc ttttgaaaac
    37741 caataaaaat acttcctgac tcagctcctc cttgtgtgat aggcaacttg atataaaagg
    37801 cgtctagtaa ttttggtgtc ccctttttta tttccccacc tttgttttct ctagaagatt
    37861 cataatactc cggtagttga ttcaacacga tatatatcgc acttagttct tcataatttg
    37921 cgtagagatc tttgatttga tttgggaaag aaactaattc atcggtactg ttttgctgtt
    37981 cgtatacata catcagatac tgatctatgg gttttgttaa gtaggtcgtc acattttcga
    38041 tcttttcgga attccctaca agctggtttt tgatgatcct actcaaggtt gttttatcat
    38101 tcagcattct gcttgtgctt tgaacggcat cacttgtcat gttttctgcc tgtttttgat
    38161 tttgttccca agtatggata ctaaaaataa cggtagcagt catcgttaca ccgatcatga
    38221 taaccgcgta tttccgcaac agacgattta gtaaggttcc ttttgaaaat agaatccgaa
    38281 acttattcat aactttccaa ctgtttatct aattcttcac gccaatgtgt tgtcatcgta
    38341 ccattccaaa ccgtcaaaag accaatgaca ccaaacagga tcaatccttt gaattgccaa
    38401 gtcacgccta aaattatacc gctaccaata attattttta gaaaagacga aaagctggaa
    38461 aaaaccgaaa taaaacttag ctttagcaga ttaggaacag agatcgtata ttcactatct
    38521 aaaatcatac tgtattgata agtagcaaat aggcagagca aggcaaaaag taaaataaaa
    38581 tcaatagcta aaaaaatgag ccctttgatt tgaacagata aatataaatt gtaactcaat
    38641 aaagcagtac cagataaaaa taaactaaat agcagattgc ctcttaaaaa atttctttta
    38701 tatatattcc agcctctttt tattgtgatt tctttatact cgaatccgta caaataaaat
    38761 agttcattga ctgttttcca tgccgcacct aatccaaaga tgatcccgcc ggcaaatgta
    38821 tatatccaga aatacaaggt tagttttata atgacccaga tccggataaa taactcttcc
    38881 aaaaccttcc ctaacatatc ttctcctcca aacattaagt ttatactttc tcattataca
    38941 aaaaaaggtt gggcaatcag caccttgacc tgattattag agaaacaaac ggaaaaatag
    39001 ttcctcatat tttgtcgttt tgtgagcgaa taccgagggt tagctgattg aacaccgttt
    39061 accaagagtt tgtgaaatag ccgcttagtt tcgagtagta agaaaaattt tgtgaatatg
    39121 gctttccaca tttttacata tatttagctt aataccgaga aactggcttt tgaacaccgt
    39181 ttattcggaa caagaggttg tgacacgtct tttgtcacaa cccctttgtc tttattcatt
    39241 attattcttg tcggaactca tcatattgtt tttgcatttc tttcaatacc ttatcatagc
    39301 cagccttgtc caaagcgtct ttcaatttcg gtaatgtttc atctggatca acagtccctg
    39361 tattcaatcc atctaaatat tgattcatga cattagaaat attacttaat tctgttttga
    39421 cactgtcagt gttgaagctg aatcctaaaa tcggtgaagt ttctgcatca gcaatcgatt
    39481 gatcacgttt ggcaatcatg tcatccgtaa tagactcttg tgtatataga atcttgttgt
    39541 ttcctgtatt ccaagcagac atatgtgtat ttggctgata accatctagc aatttgattt
    39601 tatcatctga accatcgact ttttcccatg cttctccctc gatcccccaa acaagcccat
    39661 tcaataattc tggatcactg ttaagcagcc ctagaagttc tactgatttt tctttatttt
    39721 ttgaagtatt tgatacgaca aaatttgcca tttgtgcttg agctgtagat tttagtggaa
    39781 cagtaattgc ttttgagaca agttcttgtt ttgctgcatt tgtcaaaatt gtatcgccat
    39841 aatcatacgg tccttgtgtt tctccacgca ttagccaagt atttccttct agtggataat
    39901 ccttattgct cgttgcagcg tcacttggaa tgagtccttg tttgtaccag ctatgcattg
    39961 tacgtaacag tccgatatac gtttcattat cgtattggtt gatgattttc gaatcatctc
    40021 catccagatc aatagcaaat ggtaacgtat tccctaaagg ataatcaaag ttcccttgga
    40081 ctttgaagcc ttgtccaatg gcaaaagctg ccgtgtttgg ttctttttca tggaattcct
    40141 tcaagacttt ttctgcatca gcgtaagatt ggattccatc aattgaaaga tcgtatttat
    40201 ctagtaaagc tttgttaaaa gtaagtactt gttgagcaaa gacatttcca ttgactggga
    40261 aagcatataa ttttccattg accagatttc ctttaatata tgcttcatct aaatcattat
    40321 aagctttttc agcgtactta ggcgctaatt cagtcaaatc agcaaaggct cctttttggg
    40381 cattggaaac atagttgttc gcaaaggcga tatcataatt ttccccggaa gaaatgatga
    40441 cgttcatttt cttttcgtaa tcgccccagc caatgtaatt gatattgact tttacaccga
    40501 tctctttttc gattcgtttg tttgctactt ccatcaattg atcgaaatta tctggtttat
    40561 ctccgatttg atacatctgc aaagtgtccg aattgctagc ttctgcctgg tctttagaac
    40621 cagaacctcc gcatgctgct aatgttccca acaacaacgc tgaaacacct gtaaaggcta
    40681 gtttcttcca taacttcatt tttcttcctc ctaattttat gatttatatt tttgtttctt
    40741 tattctttta ctccgccgat tgttagacct ttgacaaaat gcttctggaa aaatggatag
    40801 ctgatcgcaa taggcaacgt cgagatgact acgatcgcca ttctggcaga ttctcctgga
    40861 actgaggcta atccgcctgt caattggctt cctgctcctg aattttgtgt cagatattga
    40921 atattacttt ggattttcat caaaagatac tgtagtggaa ctagcttatc atcttggatg
    40981 tatagtaaag cattgaacca gtcattccag taacccaatg cggcaaataa actgattgtc
    41041 gcaatacctg gaacagctaa cggtaaaacg atctgaacaa atattcgcag ttcgctagct
    41101 ccatcgatcc gtgccgattc aataatcgaa tcagagaccg aacgtttaaa gaatgtgcgc
    41161 atcacaataa tattgaaggg actgacagcc attggcaaga tcaatgccca aatcgtatct
    41221 tttaactgaa gaagatttgt cataacaaga taattggcca ccatccctgg tgagaacaac
    41281 atcgtaatca aacaaaaaac ggtaaaaaaa cgacggaatg gaaagtttga ccgtgaaatc
    41341 gcataagcat ataaagaagt agccgtgcta ttgatcagtg tacctaaaac agtaacaagt
    41401 acagtgacga aaagcgcttg gaaaatttta tcctgcatct ggtcgaacaa gtacgtataa
    41461 ccaaatatac tgaacttttc tggccaaaaa ctgtacccgt gagtcgacaa tatggactca
    41521 tcggtaaaag agatggcgat aacaaatata aatggcaaaa tacaggaaat agcaaagagt
    41581 gcaatcatta tattagagaa aaaattagcc gtcttattga aagagcggac ttggactctc
    41641 tccactttct tttttttcat ttttttcctc cttagaataa tgcagaatcg ctatcaaaac
    41701 gtcgggcgat cgtatttgtg atcatcaaca agacaaagcc gacaactgat tggtaaagtc
    41761 ctgcagcagc cgtcatacca aaatctcctg tggatgtcag gccattgtag atatacgtat
    41821 ccaaaacttg agtcacatca tataatgcac cggaattcct aggaatgtta taaaacaggc
    41881 caaaatcagc tcggaaaatg tttcctacgg ctaaaatcgt taaaatcgtc attagtggaa
    41941 taagttgtgg tatcgtcaca tgtttgatct gctgccattt gctagctcca tcaaccatag
    42001 cagcttcata ataagtcgga tcgattccca taacagatgc aaagtaaata atgctgttat
    42061 aaccaatccc tttccatgta ccaatgaaca ccaagataaa cggccaatat ttcggttcat
    42121 tataccaatt gacagcttct cctccatgtc cagtgatcca ctgattgaat attcctttgt
    42181 caggacttaa aaaagcgtaa acaaagtaac tgataatgac ccaagataga aaataaggta
    42241 gtaaggacat cgtt
//
LOCUS       DAYYNG010000006        37411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000006 DAYYNG010000000
VERSION     DAYYNG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.006"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..1060)
                     /locus_tag="ACHBFA_000265"
     CDS             complement(116..1060)
                     /locus_tag="ACHBFA_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM9393792.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(1062..1748)
                     /locus_tag="ACHBFA_000266"
     CDS             complement(1062..1748)
                     /locus_tag="ACHBFA_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM9393793.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            2222..2542
                     /locus_tag="ACHBFA_000267"
     CDS             2222..2542
                     /locus_tag="ACHBFA_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9393794.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            2696..3601
                     /locus_tag="ACHBFA_000268"
     CDS             2696..3601
                     /locus_tag="ACHBFA_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393795.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            3598..4371
                     /locus_tag="ACHBFA_000269"
     CDS             3598..4371
                     /locus_tag="ACHBFA_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9393796.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(4552..5976)
                     /locus_tag="ACHBFA_000270"
     CDS             complement(4552..5976)
                     /locus_tag="ACHBFA_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9393797.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            6187..6633
                     /gene="nrdI"
                     /locus_tag="ACHBFA_000271"
     CDS             6187..6633
                     /gene="nrdI"
                     /locus_tag="ACHBFA_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9393798.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(6698..8680)
                     /locus_tag="ACHBFA_000272"
     CDS             complement(6698..8680)
                     /locus_tag="ACHBFA_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9393799.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8694..9590)
                     /gene="murQ"
                     /locus_tag="ACHBFA_000273"
     CDS             complement(8694..9590)
                     /gene="murQ"
                     /locus_tag="ACHBFA_000273"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9393800.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(9593..10654)
                     /locus_tag="ACHBFA_000274"
     CDS             complement(9593..10654)
                     /locus_tag="ACHBFA_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9393801.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(10865..11392)
                     /locus_tag="ACHBFA_000275"
     CDS             complement(10865..11392)
                     /locus_tag="ACHBFA_000275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9393802.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(11459..12961)
                     /gene="eat(A)"
                     /locus_tag="ACHBFA_000276"
     CDS             complement(11459..12961)
                     /gene="eat(A)"
                     /locus_tag="ACHBFA_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9393803.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(13219..14340)
                     /locus_tag="ACHBFA_000277"
     CDS             complement(13219..14340)
                     /locus_tag="ACHBFA_000277"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9393804.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            14466..15407
                     /locus_tag="ACHBFA_000278"
     CDS             14466..15407
                     /locus_tag="ACHBFA_000278"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9393805.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(15557..16453)
                     /locus_tag="ACHBFA_000279"
     CDS             complement(15557..16453)
                     /locus_tag="ACHBFA_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9393806.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            16722..18062
                     /locus_tag="ACHBFA_000280"
     CDS             16722..18062
                     /locus_tag="ACHBFA_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9393807.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            18280..18723
                     /locus_tag="ACHBFA_000281"
     CDS             18280..18723
                     /locus_tag="ACHBFA_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM9393808.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(19227..20645)
                     /gene="nhaC"
                     /locus_tag="ACHBFA_000282"
     CDS             complement(19227..20645)
                     /gene="nhaC"
                     /locus_tag="ACHBFA_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9393809.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(21048..22271)
                     /locus_tag="ACHBFA_000283"
     CDS             complement(21048..22271)
                     /locus_tag="ACHBFA_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9393810.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(22302..23141)
                     /locus_tag="ACHBFA_000284"
     CDS             complement(22302..23141)
                     /locus_tag="ACHBFA_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9393811.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(23158..23838)
                     /locus_tag="ACHBFA_000285"
     CDS             complement(23158..23838)
                     /locus_tag="ACHBFA_000285"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9393812.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(23840..24913)
                     /locus_tag="ACHBFA_000286"
     CDS             complement(23840..24913)
                     /locus_tag="ACHBFA_000286"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9393813.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(24969..25207)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(25250..25483)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(25560..26231)
                     /locus_tag="ACHBFA_000287"
     CDS             complement(25560..26231)
                     /locus_tag="ACHBFA_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9393814.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(26318..28966)
                     /gene="mgtA"
                     /locus_tag="ACHBFA_000288"
     CDS             complement(26318..28966)
                     /gene="mgtA"
                     /locus_tag="ACHBFA_000288"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM9393815.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(29308..29482)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            29655..30092
                     /gene="ndk"
                     /locus_tag="ACHBFA_000289"
     CDS             29655..30092
                     /gene="ndk"
                     /locus_tag="ACHBFA_000289"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9393816.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(30114..30980)
                     /locus_tag="ACHBFA_000290"
     CDS             complement(30114..30980)
                     /locus_tag="ACHBFA_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9393817.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(31140..33233)
                     /locus_tag="ACHBFA_000291"
     CDS             complement(31140..33233)
                     /locus_tag="ACHBFA_000291"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9393818.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(33982..34605)
                     /locus_tag="ACHBFA_000292"
     CDS             complement(33982..34605)
                     /locus_tag="ACHBFA_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393819.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(34764..36161)
                     /locus_tag="ACHBFA_000293"
     CDS             complement(34764..36161)
                     /locus_tag="ACHBFA_000293"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9393820.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(36193..37164)
                     /locus_tag="ACHBFA_000294"
     CDS             complement(36193..37164)
                     /locus_tag="ACHBFA_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9393821.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
BASE COUNT        10828 a         7883 c         6523 g        12177 t
ORIGIN      
        1 aatagtttaa ataacataac agatcaaact taatatattc ttataaaaaa atagtgttcc
       61 actacaatgt tagaagaggt tgcgacaatt attctgtcac aacccctctc ttctattatt
      121 taaatagaat catataagca atttggaaaa ctagcataaa taacgcaata aacaactggt
      181 ttttgatcac tccgtatttg tctttcatct ccagcatcgc tacaggcacg atattgaagt
      241 tagcagccat cggtgtcagc agggtcccac aaaaaccgca agtcaatgca accattccta
      301 ttaacgctgg attcgctcca tgactaaata cgaatggtgc accgatccca accgtcatca
      361 cggtgatggc cgcaaatgca ttccccatga ttaccgtaaa tatcaccatg ccaagagcgt
      421 aaatgatgat gccgatgttc acatttcctt ctggaacgat cgtacctact gcattggaaa
      481 tcactgtccc aactccagcg cttgtaaata ccgcacctag agaagctaaa agcattggca
      541 acatgcttaa cggccctact gtgcccagca tatctgctgc atcatctaaa aatgtacttg
      601 gtcgattatc tcgagaaaag aacatcaaga ttagcatggc tgcaaaaaca ccgacgccca
      661 cgccgactaa agcacctaaa ttggtaaata gagcaaacaa gatcgcaaag acaccgatac
      721 tcaaagctgg aataaaaatt ttcattccta gtttgtctga cattttttcc atatattctt
      781 ttgatggaag ccggctttct cctttgctga ctttcttagc aattgctggt atggtcatcg
      841 caaagatcaa gccaccgttc acgattgccg gaagccatct tcctcctgca ataactaccc
      901 ctaagaccgt ccagaaaaag gctgacccat aacgatgagg attggtttta tctgttaaat
      961 ttcttactcc agtgtaaatc aatacgagtc ccatcacgat ataaaggatc tccagtaatt
     1021 tatcgcctaa taaaatatca ggatttgtaa aaaagtccat cttattgccc ctttcctgga
     1081 taatatttct tcgctaattt cttttcttta agatagaagt agacacttgc cacaactaaa
     1141 gccgtgatag ccactggaac ttccactaaa gcaagctgtc ccaatgttac gtcatatcct
     1201 agatctttca gtgttgattg gactagcaac gcccctgctc ctccgatgaa tagtacttgg
     1261 ccaaagaacc agcagacatt ttccatagct gaagccatac cttttagttc ttcttcatgc
     1321 ttaggatgga cttctaaccc ttttgattcg atggtcccca atgccatcgg taaaatgatt
     1381 ggacgaacaa atccagcaac tccaccaaaa ctcacgttaa aagccgcaaa gatcattcga
     1441 aagatcccat aaatatctac aatcatacca gcagatactt tcttgaatcg cttgatcaag
     1501 gaagccgctg attctttcag accatttctt tcaagcgttc ctgttgccaa cataatgatg
     1561 ataaaaatcg ccatcccgcg attgtttaca aaacttgtac ctaaaagttc taacattcct
     1621 tgaatgccaa gaccgccagt caaagctgta acaactaaag caaccattac gatcaatatc
     1681 gagtctaatt taaatgcaaa cccgagaata acaatcaaga cgcctaaaag gctaagataa
     1741 ttttccatat attttcctcc ataaaatgac aatatttttt taattataaa gcaaaacaca
     1801 taaaaaatat agttgttttc tcacgaaata gacactattt ttaaatattt ttcgttattt
     1861 ttttatcaca aaaattatct tacttattgt aaggtaaagc tttttttaat ttattgcccg
     1921 ataatatata gaaataaggt ctagttactg ttttttttaa atgattaata aataacaact
     1981 tctttctatg aaattgcaaa gcattcatct tcttttccgc taacgatata tccgtcagtg
     2041 cttggtacac tttactaaaa agtatgagat tgccgatatc tggataggtg tgctatacta
     2101 cagaaaaaga tatacaatct gccaccgggt aggataaaga ggatgatcat tccattaggt
     2161 ctttgaaggg atgattttcc tctttttgtg tattttttta tagttaagga ggaatgtaag
     2221 tatggcatgg atggaattaa tcatagcagg aattttggaa gtattctggt caacgatgat
     2281 gaaatggagt gatggttttt ctaaaatcaa ttattctttt tacacagtga ttggcatgat
     2341 tgctagtttt tactttttgt caaaggcaat caaaagtttg ccgatgagtc tctcttatcc
     2401 tatctggaca gggattggcg cagttggttc ggtcataatt ggtgtggtct tgtttcatga
     2461 taagctgaat ctttccactt ggttctttgt ggggatgcta ctcatcagta tcattggcat
     2521 caagattact agcggccact agacacttgg aataagtact gatcagaaaa aataagcctt
     2581 tttatttaat attcaatttt tttatgaaaa aataagaaat aagtccaatg acctagcgac
     2641 ttctccactc atcaggtgta tgcttgtact atctatagaa aaggagtgaa tatttttgaa
     2701 aagtctttcg gtccaagaat tatccaagaa ctttggtcac aaacaagccc tcagcgatgt
     2761 cagttttagc ttatcccctg gcaaaatcgt tggattagta ggtcctaatg gcgctggaaa
     2821 aacaacgatc atgaaagcta ttttaggact gatccattac tcagatggaa caatctgtat
     2881 tggtgaaaat gagatttctc cctctagtca taaagggttg gaacaagtag gagcattgat
     2941 cgaatacccg ggtatctatc cattcttaac aggctatgat catctgaagt tattctctga
     3001 aaccaacgat gtatctgcta tcgacactat cgtgaatcag ctcaagatgg aaaagtacat
     3061 tcacaaaaaa gcgaaatcct actcgttggg aatgaaacaa aaattgggaa ttgctcttgc
     3121 cttactgaac catccgtcta tcgttatttt agatgagccg atgaacggtt tggatccaca
     3181 agcaacaaga gatgtgcgaa aaatcattac cgacttggct actcagggca cctctttctt
     3241 gatctccagt cacttactca gcgagcttga aaaaatggtc gatgagctga tcctgatcaa
     3301 tcaaggaaag atcatcaaac aatgtacaat ggctgaattg aaccaaacag cgcaagattt
     3361 catcgttatc tctacaacaa atgatctagc tgctcgaacg atcttgacag aagctggata
     3421 tactttggaa gatcctgata aaatcaagct tttaaaagaa aacgaagaat tattaggaac
     3481 cgtgatccag ctattgtctg cacaaaatat ccatgtgaca gacgtccagc atctgacgaa
     3541 tgatttggaa tcttctctat tggaaattct ggattcagta aagggggaag aagaaaaatg
     3601 acattgcttg taaaacaaga attatttaaa ttgatcaaga aaaagtcaac tgctgtttta
     3661 tctgttctat tagttgtttt gctcatagga acagcacttc tagccaaaaa atacacgacg
     3721 atcattgatc cagttgaaat gactgctcag ctttttagtg ccacatcttg gattgtcttt
     3781 atcatgattg cagctgcaag cacgatcatt tcgatggaag cacaatatgg tacattgaaa
     3841 aatctccttt atcgaaagta ttctcgagga gaaatattag taagcaaatg gatcacatta
     3901 gttatttatt ctgtttattt atacctttta gctattattg tcactgtgtt gatgaaactg
     3961 attcttttcc cttcgatttc cttcacggag aaggtttcta ctggtcaaac actgatccaa
     4021 agcttgttca cttatacttt gggtagttat atcggtttat ggctgatttt aagtttagtg
     4081 ttgatgattg cttgctttat caatagttcc ggcgcatcca tctcagcagg aatcgtcttt
     4141 tattttgctt cgtcaatcat atctggtatc cttattgcac tcatccaaaa atgggagtgg
     4201 atcaaatgga atccgatcag tatgttgaat ctgcaaaacc aaattggaaa cgaagagatc
     4261 atgaagcaat tgactcatct ttctactaat caaatgctat tcggtaattt agcttacatc
     4321 gttttgttct tagcattagg atatctcgta tttaagcgaa aaaatgtatg atgactcatc
     4381 aaaaaagagg ctgtgaaaga gacgttcagc cttattgccg attggctgtc tcttaaacac
     4441 cgtttattct gaaaaaggag ctgtgacaac atttgtgtca cagctccttt cctagtatgt
     4501 ttttgaataa acgacaggct cacaacctct ctttatactt cttttatact tctatacgtg
     4561 aagcatccca gtggcaaata aaaccattgc aatcactccg accatcgtca cacctataag
     4621 taaaatctta tctaagtgga tatgttgtac aggctcgttt tgctccttac gtgctttgag
     4681 gtaaaaataa atgcctggca agaaaagaac aagaacaagc attacttcat taaaggctac
     4741 gactagcata gcccatactt gaaaggcact tgctaaaagc cctactaaaa attggaccca
     4801 ttctttccgc tgataagaaa gtttcatttg gtacaatcct aagaaaagcc acgtgaagaa
     4861 aatagctgct gtaccaagtg tgtaagcaaa gttataagca ctgtcagtga ataataatga
     4921 aagtaaaaag acattcgtac ataacgtcgt caatacgata gcataagttg gtgccccatt
     4981 tttgttgagt tttccccatg ctttaggcaa aagtccgtct tcagccatgt ttcccattgt
     5041 ttcaatcggt aagatcgtcc atgagagcca aacaccaact aaagaaatga tcaaaccgcc
     5101 gttgatcacg actgctcccc atttaccaac catcatttca aacacatagc ccatagcagg
     5161 ctgcgccttg atcgtttgca gctgattcat cgtcattgtc ccatatggaa gcatggaaac
     5221 caagacgtaa ataataatca agccgaatat ccctagaatc gttgctttcc caacatctga
     5281 ttgttttttt gctcgtgaac ctacgacaga ggctccttca ataccaacaa atacccaaag
     5341 catcaccatg atgcaactca atacttgact ggaaaacggg atcggctgtc cattcgcatt
     5401 caatataatg tcgctagaaa agttatcaat gaattgttgg atcgaaaacg tgctgatagc
     5461 tactacaata aataaaaata atggcactaa tttacagaat gtgacgatag cattgacgaa
     5521 actagcattt tcgactcccc gattcactag ccatgcatat ccccataaaa agactgtact
     5581 catcacgatc gatgccagat tttgcccacc ttcaaatact gggaagaaat atcctaatgc
     5641 gctcatcaac aatgttgcaa aagctacgtt gcctaaccag catgtgagcc agtaacccca
     5701 accgctgata aaaccagcga aacggccaaa accttcttct gcgtaactaa agatcccgcc
     5761 ttttaaatct ggacgttttt tattgatatt attgatcgac aaggagagca tcaacattgc
     5821 tgttcctacg agtacccaac tgaaaatagc tgggccgcca gccgatccgg agatgtccgt
     5881 cataatacca aatacgccac ctccaatcgc tgaaccaatt actaaggcag caagtggaat
     5941 taaaccaatt ccttttttct gttgctctgt atccatgtaa gcatcctcca aaataaaacc
     6001 gcttttatca aaaagtataa caaacctttt ttatttttac ttaattaata aaaactttaa
     6061 tagaaatata attctaccgg cttttttctc attatacaag caaagcgcct agtccctttc
     6121 caaattacac caccaaaaaa cttaaaaaag atgctatact aaaaggaaag aattggagga
     6181 tgatccatga acattttata tatatcgatt tcaggaaata cgcgtgcctt tgccaaacat
     6241 ctagcagaat atgctgaaaa aatgcatgca gaagatcctg tcaatcctga agttacttta
     6301 aaagaaatcc atgaaaatag tgattttacg aaagaaacag aaccattttt tactttcgtt
     6361 cctacttatt tagacggcgg aaatggttta gataacggtg atacagaaat cttgaccgaa
     6421 acgatgcgtg aatatctgga acatgaagac aatcataaac tttgtcttgg tgtagtcggt
     6481 agcggaaata aaaacttcaa taatcagtat tgtctaacag ccaaacaata tgcacaaaca
     6541 tttggctttc ctttcttggc agattatgaa ttacggggaa caccatctga tgtgacacga
     6601 atttatcaga ttttagcaga aaacaataaa taataaaaaa gaagaagctg aaaaatagat
     6661 ttcagtttct tctttcgttt ctattgtttc tatcatatta agctttgaca tccatcacag
     6721 gtgcttttgc ttttgcttta cctgtttttt ccaagacaaa gttttttaca cgatccatat
     6781 tggttaccaa gacgatcatg gatgtttctt tgcctgctgc tttgatcgct tctaagtcag
     6841 cttctgctac aagcgtatca ggtgtcactt gctgcccttc tgttactttc acatcaaatg
     6901 gcgtaccgcc taagtcaact gtgttgattc ccatatgaag caagatttct aaaccatttg
     6961 gcgttgtgat cccaatagcg tgtttcgttg ggaagacaga gctgactacc ccagtaactg
     7021 gagaatagat atgtccgttt tctggttcta caaagtagcc ttcacccatc atcttttgtg
     7081 caaaaactgg atcacttgct ttatctaaag aaacaactgt tccgtcagca actgatgaga
     7141 ggatagtcac actttcagct gaagtttgag gtactacttt ttcaggcact tcatttactg
     7201 tggctgtttc agtcgctaat tcttcttttt ctaaacggtc tttaggaata ccgaagaaga
     7261 atgtagcaac gaagccacca gcataagctg ccaataatcc aaggatgtag cctaaccatt
     7321 taccgtcaga gatcaatggg atcaaagcgg ctcctgaagg tccaatagcg atagccccga
     7381 catttccgat cattccgatc actgccccgc cgatacctcc accgatacaa gcagtaataa
     7441 atgggcgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt cctaggatcc
     7501 cgactggtaa agctcctttg atcatttctg ttaattcttt atcttttttg cagcgaatcc
     7561 ataatgccaa tgctgcaccc acttgtcctg caccagccat cgcaagaatt ggaagcaata
     7621 gcgtacgacc agtttcatca atcatttgca catggattgg agtcaaaatt tgatgcagtc
     7681 cgaacataac catcggcaag aataatgtgc ctagaacgaa gccggcaaat gctccaccaa
     7741 cgccaagtac ccagttgatc ccaccgacca tgccatcaga aatgaaacct gctaaaggca
     7801 tgatgaagaa aatttcaatc agaccgatgg caatcaatga aagagttggt gtaacgatga
     7861 tatctaccgc atcaggtact actttatgca gttttttctc aacgattgac aatagccaaa
     7921 cagcgaagat gacacctagg atcccacctt gtccagctgc aagagcttca ccagtaaata
     7981 agtttttcag tggtgcttcc ggattcatcc ctgtcagcat cacaacggcc ccgataactc
     8041 caccaagagt tggtgttgca ccaaaaactt gtgcagcatt gatacctgta tagataacta
     8101 ggtagctaaa catcccgttt ttcaagatgt tcatcacatc gatatattgt tgccatgttg
     8161 ctgcatcaag tgttccggct gtgactaagt ttgctaaaac tgcggcaatc cctgccacga
     8221 tcccagtacc gacaaatgca ggaatcattg gcacaaagat attagaaatg tcttttaata
     8281 ccgatttgat tttagatggt ttttgtttcg atttttgtgc cgctttcatt tcttgtgccc
     8341 gttcgtttac acggtctttt ccagatgctg atgaatttgc tgtagctgta cttacagaag
     8401 aaagtttttc tcctaatttc acgcctgcca aatcaaccat ttgctgtgct actttgttca
     8461 ctttcccagg tccgatgatg acttgaagct gttcatcttc tactacacca agcactcctg
     8521 gaatcgcttt taattcagag acatttacaa gatcatcatt gatgatcttc atacgcacac
     8581 gagtcatgca gtggacaatg ctttctacat tgcccatgcc gcctgcccct ttataaattt
     8641 gttttgccag ttcttccaaa gtcagttcgt tttttgccat ggtcttttcc ccctcataag
     8701 gtccctttta caaatccgtt tgcttcagct aattttgctt gagcttcctc tttatcacta
     8761 tttgtcaaaa tcatgacgat agccagctta acattttgat ctgcttcttc aaagtacttt
     8821 tcagctgttt cgtaatcagc ttctgtcgct tgcatgatga ttcgttttga gcgttcgatt
     8881 agtttttcat ttgttggttt gacatcgacc atcaagttat tgaatacttt acctatacca
     8941 atcatactga tagtagaaat catattcaag ataagttttt gtgctgttcc tgacttcaat
     9001 cgagtagagc ctgtcaaaaa ttctggacct gcatcgactt cgattgggaa atccgcgtgt
     9061 ttactgattt cggcttgttt attgcaagaa atcgttcctg tgacagcacc gatcgatttt
     9121 gcgtaatcta atccaccgat cacatatggt gtacgaccgc tagctgcgat accaataact
     9181 atatcatttt ttgtcaaatt cagcttgatt agatcttctt gccctagctc ataagaatct
     9241 tcggcccctt cgacagctac agtcattgct tcttctccac cagcaattag acctatgacc
     9301 atttccggtt ctacaccgaa tgtcggtacg cattcagcag catctaacac acctagtctt
     9361 ccacttgttc cggctcccat ataaatcaga cgtccacctt ttttaaagga ctcaatcgtg
     9421 ttagcaatca ctggttcgat acttgtcagt tctttttcaa ccgcagcggc cactttctga
     9481 tcttctttgt tcattagttg aacggcttga gaaatactca tctcatctaa tccaaaagtt
     9541 tcttggtttc tgcgttccgt tgttaggttc tctaaattga ttttgctcat ttttatcttc
     9601 cttcttttct tataaattga tagttcatac cagctttgat ttggttgatc aatggtagct
     9661 cttctttgat aactttagct actacattca ctttttcatc cgctggcaaa tcatatttgg
     9721 ttaattggat ctcacccata tagcgcaaat acttttcgtt atctaaagta atagaccctt
     9781 tcatacgcgt agctgtattt cttgctggga taggcggaat ctctcgaaaa cgtgcatcta
     9841 cgcttcgtat gacatctctt gcatcgtctt gccgattcgt atgcttccct aatatatatt
     9901 cgtaaaattc ttcatctaca ggttccgtat ggagcaagac tgtctgttct ttttggaaag
     9961 aagcaaattg ctcctgaacg ttttctgaca gacctgcatc tccgatgtaa acaatatccg
    10021 tgttacttgc taatagatct agagcgcctg caagaggatg gatccctcga tgttcttcca
    10081 aagttggcaa tccttgatat agtgggcctc tcaactctgt atctcctggt acaaaacctt
    10141 ggacaaggag accttgaatt ttgagccatt gatttttttt ctgataccat tctttgtcca
    10201 gccctgtttc gggacgtgga taataattat gccaagcttc aatctgtgag aaatcagctt
    10261 ctgcttcttt caattcatcg atatctttag gcgtgatcgt gctagcattc aaactgattt
    10321 tcaattgatg tgaccagtcg gcaatctgct gattggaaat atgataatcc attcttaaac
    10381 ctgttacacc aattgctttc aaaggcgtta gatcttctaa tgaaaaaccc gctcgttcca
    10441 atgcctctcc tgaaatatcg accatcaatt ctagtttatt ctttttcgct tcgcttccta
    10501 gttcgatcaa tcgcttttga tatgccgaaa catcatcttc tggtatatgc atggaagtga
    10561 aaataccgac aaagccattg gatgccattt ggcggatata ctgtttcttc tcttctgaca
    10621 aagaatcgtt cataaatatt gaaaagccaa acatattatc cctctttttc tgtatccgtc
    10681 ttcatttcta tgactagagt atatatctaa agaaacaaaa tttcaataat tttattaaaa
    10741 taattaaatt tcatttctaa aggttgagtt tatattatca aaagaattgt cataacctaa
    10801 aaaaggcact gctaagcaaa agcagtgcct tgaaatattt tatttaaata acacaaaaac
    10861 ataattactc ataagaaacc aattctgtat tatctaccat gaatgtaaag aacccctcat
    10921 taggcatcgg atgaaaataa gcattgatca ctcttatcaa ggcattatgg caaactaaca
    10981 gatagacatt ttcttcgtct tcgatgcatt ctttcaataa aggaacgatc cgtgcataca
    11041 catctaagac tgactcccca tttgggaatc gtacagcgaa cgctaaacgt gctttttgaa
    11101 agttctcatc tttgcttggc agtccatcat aatccccaaa atccatttct accaatcgtt
    11161 catcaacggt cagcggcaag ctgagacgat cagcaactgc ttgtgctgta tctcttgctc
    11221 gttgaagagg ggaatggatg attttcgtga ttggaacagg caaatctact accttttctg
    11281 ctagtttctg tgcctgcatc tgtccttttt cagtcagcgg cacatctgcc cgtccgcaaa
    11341 ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc atgtgattct
    11401 ctccttcgtt ggcaaagttc cccttaacca taccttgttg ttcttgagat tacaagggtt
    11461 agattctctc aagagaaata atctcagtag atactttatc caggaaggtt tgatcatgtt
    11521 ccactagtaa catggcaggt ttcacgttca agatcagttg ttctaattgt tcttgattga
    11581 agacatccaa ataattcaat ggttcatccc atgtatatag ttcagctggc tgtgataaag
    11641 atttagccaa ttccactttt ttccgttggc ccatactcat ctgctcgatc ttgttatgaa
    11701 aaacatctct ttccatccca agctttcgga ggttgttcaa aaatgcttgg tagtctactt
    11761 ggtttttctc tgcaaattcc gccaacgttc ctcgattgtc ttcataattt tggcttgcat
    11821 aactaatgct cagatgtttt ggctggtatt tttctcctat aaccttgccg ttgaatgccc
    11881 ccagaagata atggataatg gatgactttc ctgcaccgtt aggacctgaa atcgccaccc
    11941 gctgatgagg ctcgattgta aaatgaattg gctcgaataa cagattttca taccctaatt
    12001 gaagatcttc tacgcttaaa agtcgcttat ggtgagacgc ttggctattc atcgtcaaag
    12061 aatcgatata ttcgatatct tttaatagtt tttctttctc gctgatctgg gtctccatcc
    12121 gattcacgat tgctttggat cgtttcatcg tccgtgcagc atcagcaccc actgctcctt
    12181 tattcactcg tctagattca gtatcgatga atccgacttg tttctttgtt ttatctcctt
    12241 ctcgggaacg agaccattcg gctttttcag ctgctgtttt ctttagccta ctgacttctt
    12301 ttttcaattt ttcattttga gccatttcaa attcatcacg aagttttttc tgttcttcat
    12361 agatagagaa attcccttga taaagttcca gttgactttt ttcgattgct aaaacatggt
    12421 ccactacttc atcgataaat ccccggtcat ggctgaccac gatgaagcct tgttttttct
    12481 ttttcaaata agccgctact tgttttctac cagagatatc caaatgattc gttggttcat
    12541 cgattaacgg gaaatgagtg tcatccacaa ataaaagtgc cagtaggact tttgtcttct
    12601 ctccccccga tagtgtgctg aattctctcc ataagatttc aggatctgtt tgcatcaatt
    12661 ggagctctct ttcgatttcc catatctcaa aatccgtaat atcatttaac acgtaatagg
    12721 ttaaacgttc tttatctttt gtctgttgcg ggaaataggc aaattcttgc tgatggatta
    12781 cttgcccttg ataaggtagt ttgttttgta gaatattcag taaagttgtc tttcctcgac
    12841 cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa atttagattt gcttgttcaa
    12901 ataataatgt gccttggctg tcgtagccga atgtcagatt ttttatttcg attttagaca
    12961 ttttccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta aagaggatgc
    13021 aagggttgga tacacacaaa aagacccata catcgaaaaa accaagccaa tcccgaaaat
    13081 ctgcacactg cttccttgtc tccacaaggt acagtctgaa tgcagctttc atcaatcgac
    13141 ttaatttttc aatgcacgga tccctcactt tgtcctatat ttgaaattac gatagcataa
    13201 agaaaatgat tttgtcaact acttcgggag gatggtgaaa aatctgctaa gtgtctgtcc
    13261 taagattttt gttttttcta atgtttgcct gtgttccatt gcttcttcta aacctattgg
    13321 acccaaatga atacttaaga caattgggag aaaatcagaa agtccttcta gctcttggtc
    13381 tctacttccg caaatagcga ttattggttt tcgatatttt tttgcaagca ctgctacacc
    13441 gtatggaact tttccgtgga tcgtttgctg atcgatccgc ccttctcctg taatgatgac
    13501 atctgcttgt tcgatctttt tttccaatcc agtgatttgg ctgactaaat cgaaaccaga
    13561 aaccatcttc gctcccaaca aaaaaagggc tccaccaata ccgcctgctg ctccacttcc
    13621 tggaatatca tctaaatcca cctcccagaa atcttttatt tgctgagcaa tctttcctgc
    13681 ttgtttatct aagaaagcta gcgtcgtatc tattcctcct ttttgtcttc caaattgcca
    13741 agcagctcct tgttgacctg tatatggatt ggtaacatct gcagcgataa tcaattttac
    13801 tttagaaaca agaacgtttc ttcgatcaaa atgaagtttt ttagtagata ataaaggatt
    13861 tccttctgtg tacccttcta aagaagcacc caaggattgg agtaatccta gaccaccatc
    13921 gctacacccg cttcctccta aagtgacaat aatttgttct acttcttgaa gcagtgcatc
    13981 tttaataagt tctcctaagg cataactgga agcaagtcgg atagtttgat ccgttggttt
    14041 atcaagtaga tctaacccca atgcttgtgc agattcgatg actgcggtgt tttttccttg
    14101 ccaagttgtc aataaatagc tgatttttcg ctgacggaat aagagatcca tcccatcaat
    14161 ggtacgccat gttcccctga gcgcgtaata gattgcttct gcagtatttt ctccgccatc
    14221 tgcaatcggc acattgattt ttcttccttt acgatcttcc cagccattta gaactgcctg
    14281 atttagttca aatgaagaag cacttccttt aaatgaatca attgccgcca aaatatccat
    14341 ctgtatccct cactttcttc ttaagtaaga cacttttttt catttttttc aaacaaaaca
    14401 atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata aaggagagaa
    14461 aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc ggattgatgc
    14521 tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac attgaagcca
    14581 ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca aaactgccaa
    14641 tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc gtagcattaa
    14701 caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt actgacaata
    14761 cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa aaatatgtgc
    14821 caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct ggtcccggaa
    14881 aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca gaaggcgaag
    14941 catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg atctatagcc
    15001 ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg aatggtttat
    15061 gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca gagagcatgg
    15121 ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc atactagatc
    15181 aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc gggttgcatt
    15241 acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc gactacatca
    15301 atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat tgtgcctttt
    15361 tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag gctctctgat
    15421 gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa gatcaaaaag
    15481 aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta cataatctct
    15541 tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg tattgctcat
    15601 aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca taaaattcca
    15661 atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat tcatctaaat
    15721 cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga gcaatcgctt
    15781 gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc aaagtcactc
    15841 cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt tttttcaggt
    15901 actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga atgaaatagt
    15961 tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt tcttttctcc
    16021 cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa aaattcatat
    16081 gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc aaaaaatcaa
    16141 aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc gcctcgtgtt
    16201 ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat gggcgcacgc
    16261 gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga taatttgcca
    16321 actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg gaccaaacat
    16381 ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact agcagtacgt
    16441 catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg atagcctgtt
    16501 taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta acatttgtat
    16561 gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt ttctgaatag
    16621 ttgtaattgt ctgaaaattt gttatcatta gtcattgtta aatattgcta tttattctta
    16681 atataaattt tgattgtgaa tttaaaagaa agaggtcatt tatggaagat aaaaaattga
    16741 aaaaacaact tagctccaga catatcacca tgttagcttt aggtggagca atcggtgcag
    16801 gtctatttaa aggaagtggc gaagcgattg ggatcgccgg cccttctgta ttgattgctt
    16861 ttttgattgg tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa atcgttttaa
    16921 gcggtggcga cacgcatcac ggattatctg gtttgatacg cccttatcta ggtgctcatt
    16981 ctgctgattt tactgattgg gtctactggt ctatgtggat tatcaatatc atcgctgaag
    17041 ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca cattccagcc tgggtatttg
    17101 tttttatgtt ggctgtttta actacgatca tcaatcttta ttcggtacgg ttattcgcag
    17161 aaacagaata ttggctggct tttgctaaaa tcagtgtcat tatcttactg atcatttttg
    17221 ctacttatct tgtcggtcag caaatgttgg gcacaggcgt tttccctacc cttcagcaat
    17281 tgactgacca tggtgggttt actcctcatg gtatgaaggg aatcatcagt tctttgcttg
    17341 tagttatcta ttcttacggc ggttctgaac tgattgctat cacggtaagc gaagcagatg
    17401 atcctaaacg tgcgattcct aaagcaatca aaggtgtcat gggaagaatc atttcctttt
    17461 acatcattcc tttgtttttg cttttgattc tttttccttg gaatacttta gcaggcacga
    17521 atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat cccatttgca gcggatatcg
    17581 tcaattttgt gattgtcctt gctttgttct catctatcaa ttcgggtgtg tatgcatctt
    17641 caagaatttt gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa ctagctgttt
    17701 taaacaagca tcaagtaccg caacgtgctg ttttattttg taccagtgtt ctttatcttg
    17761 gtgtagctct ttcttatttt gtcggtgaca aattatttgg ctatctggca ggttcccttt
    17821 cgtatacagt attattgatc tggatcatta tcagtgcggc atcctttgta ctggcactaa
    17881 gaaaaggaac attatgggat aaaggaatca gcttttttgc attagctgtc ttaggattga
    17941 ttttccttgg tatcctgttc acgaactcca ttggggtaac tgtattgact ggcctgcttt
    18001 acctgttcat ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg acgaatgaat
    18061 gaaatcgata gaaaatttta ttttatttat gtggaataga tggagctatt gaagaaagga
    18121 gttgtgacaa aagtcttgtt gcaactcctt tttccgaata aacagtctca tgacctcttg
    18181 gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat tgttctccat gctcatgaca
    18241 tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa tgaaaaagta ttggaagttt
    18301 ttgttgatga tcggtgtttc aacggtagtt atgtttggta tgatgtactt gaatgtctat
    18361 gcgctcgatc atatcttctt cagtcgtaca cgagtattca tggcgttgat gatgggtgca
    18421 gtcatggcag tcatcatgct gttgttcatg tggaaaatgt atgaaaacaa aaagataaat
    18481 gttatgatct tagccgctag tcttcttgtt tttgccggtt ccttgtttat ggtacgaagc
    18541 caaactactg tcaacgacac agcttggatg aaggccatga ttcctcatca ttctatcgct
    18601 atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag taaaaaaatt agcggaagaa
    18661 atcattcagg cacaagaaga agagatcgac aaaatgaaaa aaatgatcaa agaaatggaa
    18721 tgaccattat ccgtccaaat aaaaaaagag gatgttccag aagttatcag cttcaagaaa
    18781 taagaagaag ctacttctgg aacgccgtga ataaggggtt gtgaaaaagc tgccctgcat
    18841 caagtaataa gaacagccaa acaaaaatag cttctcctat ttttcgtttg gttaggctta
    18901 ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtaaaat cagcgttctg
    18961 acctgagtat tagagagaca aatggaaaaa tagttcctca tattttgcca ttttttgagc
    19021 taataccgag ggtcagctga ttgaacaccg tttatcgaga ggttgtgaaa aagtcgctta
    19081 gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca catttttgta taaatttggc
    19141 ttaataccga gaaactggct tttgaatacc gtttattcgt aaaagggatt gtgacaaaag
    19201 tcttgtcaca atcccttttt atgtttttat tgttctaatt gcgctgtctt gcttactgct
    19261 tcttgcggtt tcacaaattt ttttgagatt ccccaacctg tgaaggcact gaccatcgaa
    19321 aacacaggag aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt aggaatacca
    19381 aatgtacttg ctacgaaact tccggcaatt ccccaaggca ctaagtagtt gattactgtt
    19441 ccaccatctt ctagcgttct acctaataca gttggatcaa atcctgcttt ttcataagtt
    19501 tctttaaatg cattaccagg cagaataaca gataggaatt gttcaccaat aaaaatattt
    19561 actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg gtgtgttcgt catctttctt
    19621 gatactcgtt ccatcacggt tgagatcaag ccaaattcga tcaacaatcc acctaatgac
    19681 aaagttagaa taatcaatga cacagtaggc atcatacttt cgattcctcc gcggctaagc
    19741 agtgaatcga tttgttggtt tccagtattt gaaacaaacc ctgattcgat gaccccagcc
    19801 aacttcgtta aggaagtttg tgggttttga ataaaaatca ttcctactgc tacagcgata
    19861 ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg cacacagtaa cattaaaaat
    19921 aaaggaatca gtgaccagat tgaaatagta aagtttgctt ctagaacatc agtcacttca
    19981 cgaatagatg aaagacttgc ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa
    20041 gaaacaacaa atgccgggac agttgaccac atcatatgct tgatgtgttt gaacaaatcg
    20101 gcatcaacaa tagctgctgc tagatttgtt gattcggata atggagacat cttatctcca
    20161 aaaacggcac ctgaaacaat agctccaaca accagcgctg gatttaagcc tagtgttgtc
    20221 ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact ccctacagcg
    20281 ctacctacga ttgcacacac aacaaataca gaagggacaa accactggac actgatcaat
    20341 ttgaacccaa tgaccattag agttggaatg atccctgctt gtatccatac agcaatcaat
    20401 gaaccgacta aaataaaaat aaagattggg atgattcctg gtttgatgcc attgatgatt
    20461 ccttcgtgca gccaatctgt cgggtatctt tttatcactg catacaacat gaccattccg
    20521 atcgctgcta agatcgtgat attaggtgag acctttaatc cgacaacgcc aatcgcaatg
    20581 cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa aacttggtgt tccttttttt
    20641 cccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta agatatcgga
    20701 ttactccgcc gcaattcatc cgcgattcct cctttataaa aataaaaaga cctgttttaa
    20761 actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt ttttcacttt
    20821 caaactttta ttcaaatgta tggactttcc atgtaattcg tcaattaggc ttgatcggtt
    20881 cacagcgacc accgactttc tgaacaaaac acctaaccgc tactgcgtga aaagttgact
    20941 tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata ttttttcatt
    21001 tttctgaaaa ttatttttat aactttcaca cacaagttta ttttttacta gaaagtagcg
    21061 ccctcttgtt tttttcttgc tactatttct tgatgttctt gcttgattct ttctaatagc
    21121 tttggctctt tcaacagatc aagagctgta ttggcgagta gttctgctgc aatcgcaata
    21181 gaagcaaggc ctttgtcact tttagcagca gctttgaatt cttctgtgtg tccggcaata
    21241 tatgtctcag agattgctac cgtaggctgg attgttggta tgacttggct gacatttcct
    21301 acatcagaag aaccgagact tgttctttgt tccgtttcta tttcttcatc tggaacacct
    21361 gcttcttttg catgctggaa gaataagtca tcaaaagaag gagtaacgat gacatcatca
    21421 actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc agcacctcgt
    21481 acgatatttt ccactttttc atatacttta tctaatgttt gccgatttcc tgcacgtaaa
    21541 tagaaacgtc cagcagcgta atctggaatg acgttggcag ctactcctcc gtctgtaatg
    21601 atcccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat tccattaaac
    21661 acttggatca atgcttccaa agcatttact cctttttcag gtgctgctgc tgcatgggaa
    21721 gcgacaccgt aaaatttgat gtcgactgga tcattcgcta aagattctgt cgttttccca
    21781 taacgaaaag ctggatggac gcataacgct acatcgacat cctcaaaaaa accttctcga
    21841 acaaagctgc ctttagctga tccgttctcg cccccttctt cccctggggt gccatataca
    21901 cggatttctc caccagtttc atcaatgaat tgcttcacta tgctggcggc taatacggag
    21961 taagtaccaa acaaattatg cccgcatgca tgaccaatac ctggtaaagc atcgaattct
    22021 gccaaaaagg caattgtggg accgggttta ttggaaatat aacgtgcatc aaaggctgtt
    22081 cggtgtccag ctacttcttt ttctacccaa aacctttctt tttctaattg tttaatcaac
    22141 gctgctgagg aataaaactc atagttactt acttcaggat gttcgtggat atctagagca
    22201 agtgcttgat attctggtaa acgatattgg attagatcct tcaattggtt tgttgagatt
    22261 gttgttgtca taaaaaattc ctcctatgct agtcattttt cttattttct cccgaatgtt
    22321 tcccatgcag gcacgcttga tccttttgat gttttctcga ttactttgac tgtgtcttct
    22381 gtttgataag cgtctactat tttttgatac gttttattgt tttcatcttc ttttcttgca
    22441 acgatgatat tcacataagg acgtgcatgg tcatccactg gttctaagaa gatggcatca
    22501 gaagcaggat taaatccagc atctacagct acaccgctat tgatcactga aatgtctaca
    22561 tctgacaaag cacgtgctgt ttgggaagca tctaattcag aaatgtctag attttttcta
    22621 ttctcggtaa tatcagaaac agtgggcgct tgtccttttg ccttatccac tttgatcaat
    22681 ccagcagttt gcaataacag aagagctctg ccgccatttg taacatcatt agggattgct
    22741 acttttgctc cgtctttgat atcagctaca tccttcaaag tgttagaata aattcccagc
    22801 ggtgcattga cagtatttcc gatcgatact aaatcagttc cgtgttcttt attgtaattg
    22861 tctaaaaaga attgatgttg gaaagaattc aaatcaatcg atccatctgc taaagccaca
    22921 tttggttgat tgtaatctgt aaatttcacg tattccaaat taatgttctc ctttttcaaa
    22981 cggtctttca cgttgtccca tacatctgta tcttctccaa taatccctag ttttacgata
    23041 gtttcttttt tgtcagttgt atctgctttt ccttggctac accctgccaa ccaaataatt
    23101 gctacaaatg taatgactac aaaacgaata atttttttca ttttttctcc tccactttca
    23161 atgtgtaatt tttttaatca gttgattact gataaattga cttaaaaaaa ctaagattag
    23221 aatcaataat gtagccacaa tcgttacatc tgtttggaat cgattatacc ctctcgatat
    23281 agcaagattc cctaatccgc ctgcacctac tgctccagcc atggtagtca gtccgatcac
    23341 gttgatgata gtcaacgccg agacccgaat gataccaggc aaaccttcta acaaatacac
    23401 acgtaaaatg ataccgattg gactggttcc catcgcttct gccgcttcga tcaccccagg
    23461 atcaacttct agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa ctccaaaaat
    23521 caatggcact aaagctgctt tcgcaccaat cgtcgttcca actaagaaac gagtagtcaa
    23581 tgctaaaaga gaaagcaaga taatgaaagg gatggaacga atgatattga tcagcttatc
    23641 aagtatttca aatagcagac ggttagctag taatccatta gggcgtgtca ctactaacgt
    23701 cacacctaag atgattccaa tcatcccgcc aatgaatgct gtccaaaaag acatatatag
    23761 tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa taaacattgg gcaaatatgt
    23821 tttaaagaaa tcagtcattt tatcttcctc cttcgatcac tttgatacga ctatctaaat
    23881 cgtttttttg taaaatattt atttttacac cagatttgat caaataatcc aatgcttttt
    23941 tccgctgttt ggtttctcca gctaaagtaa cgatcaaact ccctaacggc gtttcttgta
    24001 ataactctac attgccatat aaaatattcg ctgttacttg atatttgctg tacagatgag
    24061 cgattagagg ttcgttcgtc tgatttccaa tataacttaa ttccaccagc catttattgg
    24121 cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg tcaagatgag
    24181 tagctgttcg aataaaatct ttggtcaatt cttcttcagg attgctgaag atctggatgc
    24241 tgctaccttt ttcaacgatt cttccatctt ccataaccgc taccttgttg cagatttctt
    24301 tgacaacttg catctcatga gtgatcagga cgattgttaa acctaactgg cgattcaatt
    24361 tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg gttgcttcat
    24421 cacataataa taccttaggt tcattcgcca aagctcgtgc gattgctact cgttgttttt
    24481 gtccccccga cagttgttta ggataagcag atgctttttc ttccaaaccg acaagttcca
    24541 gtagttcatt cactttttga cgacgttcct gtttcgattt tccagaatac tttaaagaaa
    24601 aatcaacgtt gtcaaaaata gttcggcttt ccattagatt gaaatgctga aagatcatac
    24661 cgatggcctt tctttctttt cgcaattctt tgggagagag ctgactcaga ttcgtctgat
    24721 tgacaatgac ttctcctgct gttggtttct gcagcaaatt catcacacga actaacgtac
    24781 ttttccctgc acctgagtaa ccgacgattc cataaacatc gcctttttca atcgttaaat
    24841 ctacagaatc tactgcctgt atctcttttt ctttttgttt gaaacataca gaaacatctc
    24901 gcaattcaat cattaaaatt cctccttatc taaaaaaagc gtcctcaaga aaatgaaaat
    24961 gtcattttct tgaggacgct tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca
    25021 cacaactcct cttcaagtac gatcatactc tagcaagata tcggtcgcag ccgtcatcgc
    25081 cttacatcta tgctcgggat gaattgctcg atggccattt tccaatagtc gttccttgct
    25141 tcttctcagc taccgaagct ctctgtcaaa ggttcttcta tcgtacttac catttcaatg
    25201 caaataatat tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt aaaaggacga
    25261 cgtatcgcgg taccaccttt tttcggaagt ttccttccct caagcccaac atctcaaaaa
    25321 gagagattgg cgttcgtttt aacggtcgaa cagttccgaa tttgcctact tatcgacaaa
    25381 ttattctcca aggccatttt cagcttttct tacttgactc ttctcaccag ccagagcctc
    25441 tctgcaaagt ttccaaactt actttccttt tcaacgaaat cgatattttg ttgttgggat
    25501 taatctacat gaggaaaact gaaaagtcaa gtacgaaatt caaattattt tttctatatt
    25561 tatttttctg tttttttatt taacatttca ataacagttc gaagatcttt tccttccgca
    25621 agaagtttca atttttcctt ttcttcgacg gtataatttc ggacaaaact ggaatcaggt
    25681 atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa agaatcttcc
    25741 actgcaaagg ctgaactagc ttctaatcct gttcgttcca aagctgtcag ataaggaatc
    25801 ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa gtaatcagct
    25861 aatttaaagt gatccaagat atctgttgct cttttcgtaa cagtggtcgt agcaatgcct
    25921 agtttcatat gacgttcttt taagtctgcc aatatttctt ttgcatatgg ttttaagtgg
    25981 atggctccgc ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg gacttcctgt
    26041 actttttcag ggctacctgc tgctttcact aaaatatcaa aactttgttt ccagtttttt
    26101 ccgccccaag cgtcaacgat ttcttttggg atatggatat caaattgctg aaatccccaa
    26161 agccagccat cacgatacgt tttttctgtg tcaactaaca aaccatctaa atcaaatatt
    26221 gcacctttca tctatacatt aatcctttcc aaaacaatac acatagttta gcacgactgg
    26281 tccaaaaaga acagaagcaa agaaccagcc ttctcgttta taagagttct tggaatcgac
    26341 ggatatagat tttcttcaca gctgtcacaa gcgccagata agcgatgact gtagcaaata
    26401 accataacca gtaattgcca ggaagtggca tcaagcctaa ttcagcacca aaacttgtaa
    26461 aaggaaggac agaaccgaca ataattccta atgtagtgat aacaaacatg gcaaatgttg
    26521 cgttgctttg gataaatggt actttcggtg tacgcagtgc atggagcact aaggtttgtg
    26581 tccatagtga ttcgacaaac catcctgcat ggaataatgc gatgaaaatc actcgctgtg
    26641 aactatccaa tgtatggaaa cttccgccca ctacagcagg acaaataaca aaatacatca
    26701 acaagtaagt agtaatatca aatatcgaac ttgttggacc aaagtaaacc atgaattccc
    26761 caatactaga tgcattccat ttttttggct cgtccaaata ttctttatcc atccgatccc
    26821 atggcaagga aatgcaggaa acatcataga tcagattcaa aaacaaaagt tgtaaaggca
    26881 gcattggtaa aaatggcagg aacgtacttg caatcagcac ggaaaacata ttgccaaaat
    26941 tagaacttgc tgtagccttg atatatttca tgatattccc aaaagtttct cgtccagaaa
    27001 gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct gcagattctt
    27061 tcgcaatatc cacagctgta tctacagaaa taccgatatc agccactttc attgcaggag
    27121 cgtcattgat accatctcct aaaaatccga cagtatgacc atttttacgt aaagctgtcg
    27181 tcaaacgtgc tttttgctga ggattcaatt ttacgaatac ttcatggtct tcagctactt
    27241 gtgctaattc atgatcactc atttccgaga ttttctcacc ggtaaccaat tcattgatct
    27301 ctagacctac ttgacggcac actgaacgtg taactaattc gttgtcccca gtcagaactt
    27361 ttacagctac accatgttct ttcagagctt tcaacgcttg tttcgttgtt tcttttggtg
    27421 gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga ctcatctttg actgaaaatt
    27481 ctccaacgac gcttggattg gtcttttgtg cgacaccaat cactcgtaag ccatcttcat
    27541 taagtgactg tacttttgca agtacggctt cttttcgttc ttttgttaat ggtgtaacag
    27601 taccgccaag atcaataaat ttagaaatgt tcagcatttc ttcaatcgcg ccttttgtga
    27661 tcatttgtgt tttaccagct ggatcagcaa ccacaacact catacgacga cgttcaaaat
    27721 caaaaggaat ttcatccact ttttgataat tgatcttatc tgtatctaaa gtttctgttg
    27781 cttcatcaat gatagccaca tctaacaaat ttttcagccc tgtttgatag tagctattta
    27841 gatacgcatg gcgcaaaaca cgatcatctt catttccttc acaatccata tgatattcaa
    27901 gaatgatttt atcttgtgtc aatgtccccg ttttatccgt acataacaca tcgattgcac
    27961 caaagttctg gatggcattc aaatttttga tcactgttcc ttttttggcc atcacacttg
    28021 ctcctttgac caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt agaccaactg
    28081 ctacagatag tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc ccattgatga
    28141 cgataactgc tggagccatc aatgacataa atttgatcag tagcatggat gttttatgga
    28201 tgcctaattc aaagcttgat tgaattggtt tttcagatag cgtttctgct acatgaccaa
    28261 atagtgtttc atttccaaca gaaacaatga ttccttccgc acttccactg atgacattac
    28321 ttcccataaa gacaagattt tcgtaacttg tttcgctttg ctgatcgtct tcacatactt
    28381 tcgcatgctt ttctactgga tagctttccc ctgtcaatgc tgcttgggaa atgaataaat
    28441 cctttgcgga caataaacgc atatccgctg gtatcatatc ccctgctgaa agccgaacaa
    28501 gatctccaca gacgatttct tccataggaa tctctgtgta ctttccatta cgttttacag
    28561 ctgccgttac tttcacaagt gatttcagtt tttcagccgc ttgattggaa cgtacggact
    28621 gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa gataatggct acaccgaaaa
    28681 gatctttatc tccaggagct gctaatacat attccgtgat gaatgaaata attcctaatg
    28741 cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa tatttctaca atgaatggtg
    28801 tttttttgcc atatgaaatc gcattttcac cgtaattttc tcttgaacgt tcgatttgtt
    28861 cttctgtcaa tccatttgct gatgcatgga atagttcaaa taattcttgt ttcgtttttc
    28921 cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt ttccatctca atctctcctt
    28981 tgtgtccaaa agacagctgt tctcgctgct tttggtcttt agctattcat cggatcgata
    29041 gagaaaaaac tgaaaggggc tgagaacaga tagataatag ccctggcctt tcctctagat
    29101 ccttgttttt cgaatgactg tcatccattt gttctcatct cctttactaa gtaattttaa
    29161 ttcatgactt ccaatcattt ttcgaatagt tatttttcca attagaccac ctccttttta
    29221 tttttcaccg atggggattt gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag
    29281 gtttgaaaaa gcatattaaa aaaagcttct cagatctcat cgttgagttt tgacactgta
    29341 caacgtaaaa agcagatacc tgcttttagc taccttatga aaagccttat ctcgacaatt
    29401 cctgttctac ccattggcgt ctctcgacgt ttttgggcag tagcctatgt ttgtatagga
    29461 gcctcaccta acaaatctta ttcggttttc cttcgttaat ataacaacta aaaaagagga
    29521 tgtcaaatca attttccatg tttttcatac tttttttaaa atacacacgt tatattcctg
    29581 tctcacgcgg ttttatttgt ctcattttcc acctcccgct ataatagaaa tatagtagga
    29641 aacgaggtgc tgttatggaa agaacacttg ttattatcaa gccagacggc gtgagacgcc
    29701 atttagtcgg aagcatcatc caacgttttg aagcaaaggg actggccatt gctgaaatga
    29761 agtttgaaac tatgactcct gaattagcaa aagaacatta tcagcacttg actgagcgca
    29821 gtttttttga tgaactgatc gattacatga cttccggtcc tgtcgtttat cttgtgctgg
    29881 tcggcgagga agtgatcgat atcgtccgta agatggttgg agcaacaaaa gctgccgatg
    29941 ctgttccagg tacgatccga ggagactacg cgctgccggg gactgaaaac atcattcacg
    30001 cttctgattc ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg ccattcgata
    30061 taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct ggtctagttg
    30121 ctgggttcag caatggccag cagttttttc tctaaggacc tccgttgatt ctttcctaac
    30181 atatattcga gcactgtgaa gaattcttgg tagtctttcg tcttttcttc ccctaaagca
    30241 gcagaaatca atctttgata ataatctgcc atcaatgttt catagatcac tgtacttctt
    30301 tctttatgtg ctaagctccg tttcatatct tcattcacac tacgtgcctt tttcaaccga
    30361 ccattttcta tataaaacga agtcaggttc attaaaaaag taaagagata ctgctcatga
    30421 ttgttcaact gtgctaatcg tcgcattctt tttttgattt tcaaataatt gttctcaatg
    30481 aaccgatcac tgaatatcca aggaaaatca gctagaatag atagttcaaa ttgaccatag
    30541 tctggtatct gcatcaagta ttcttcaatg attcggcatt cataacgtaa aaattcaaat
    30601 tctgtctcag atacttctgt tttttcgacc atgtgaagat tcaaaaccaa ataatacaga
    30661 tcttctgttt tcttaaaatg cctacggtta ttgctgatct ctttataagg atcaaagctg
    30721 tcttcttgta cccgatctag cattcgtctt tttttcttta actttattgg ttcatccagc
    30781 tggtttcgca tccattcata ttctgcaagt gtcatgttca ttcgctctaa aaatgatctc
    30841 aatgttacaa atgacatttt tttcactgaa tttgtctcaa tcccgactag agaagtctta
    30901 aaacagattc cttccgctaa ttgggactgt gtcaacttct tgttgactcg aagtttcttg
    30961 atcaattcac catcatacat aatagttctc ctttttaatt taaatttttc cacattcaat
    31021 aaatagatac gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt tttttcataa
    31081 aaaataccaa ccttcaagca ttagaatgtt gtctaactac tgaaggttgg tttgattgtt
    31141 tattgattat ttttttgctg ttcaacgtct gttcttgtta ctggatataa gttggttgtt
    31201 gttttattac cattttttga aaagacactt gtcgaatgtt cgcctttttg cttttcgatt
    31261 gcttcattac gttccgcttc atttttatag tcatattttt tcggatcaat cgtctcaaat
    31321 ccgtttggca cataaaaccg caagagattt tcctgattca gcttatctga aagacttaac
    31381 gcagtcttga ctttttcctg atctttggag atttcctcat cgattcctgc tgctttcgta
    31441 tcgatgattt ctcctgtctg attattgtaa gcttttcctc ctaggacggt gtatttgggt
    31501 gtcacaaagt tcccatttcg aaaagcaact acttgatcat gctgagaaga taataagtct
    31561 gtaccaaaat gaagataatc tttatcatcg attcccaata gatgtagtaa tgtcggcaaa
    31621 acatcgattt ctccaccgta ttcatgattg actgtccctt tagtataacc aggcatatgg
    31681 atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt catcccatgt atctggatct
    31741 ttaccaagag cgcttgctaa atctttatta tctgtattcg agatcccaaa atgatccccg
    31801 taaatcacaa agattgaatt ttggtaaata ccagattttt tcaaatacgt aaaaaattgt
    31861 tccaatgatt gatccagata atgtgccgtt cgaacataat tatctactgt gctattgcca
    31921 gtattcaaag atggaaagtc gaaattcttg tcatccgtat aataaggcgt gtggttggtt
    31981 acggaaagaa atttcgtgta aaacggctgt tgtagatgct caagatattg ggcagattct
    32041 ctgaataaat ctttatctaa tatcccataa cctaacgttt cgtcggattc atcaaaataa
    32101 gatcgatcaa agaaattgtc ataacctaag tttttatata catgatctct attccaaaaa
    32161 ctagctacat tcccatggaa aacagcgctt gtatagcctt gcttttgtcc aaggatcgct
    32221 ggtgcggctt gaaagacatt gtctgaccca agttctgtga ataatgagcc ttgaggtaaa
    32281 ccaaatgtgc cagtttccaa catattttca gcatcacttg tctttccctg tcctacttca
    32341 tgaaagaaat tgtcaaagct gatcgttgct tgattctgga aaatactatt taaaaacggt
    32401 gtgacttctt gtccgtctac cttcatatta atcaaaaact gctggaaact ttctaaatgg
    32461 agaacgatca catttttccc tttagcgatc ccaaatgttt cgggatttgg ctctgcataa
    32521 tgtttctttg tataatctaa aacttcatca atcccattac tgcttgcatg cgcgcgaaca
    32581 ctgctagtca ttcctgtttt gattccgtca taaatagtat acgcatcgat tcctagatat
    32641 ttgacaatat atgaacggtc aaatgttcgc tgcagcaatt gtggacggtt ggcttcgctc
    32701 agcgctagat ttcctgaaaa aataagccct gaaagacagg taacagcgat agccatcggt
    32761 ttgccgacag ggttgctttt gataggtatt ttcttgacct tcaaccaaac cagtagccca
    32821 atgaacacag cgatatccag ccagtagatg acatcatggg gcttcatcaa agaaaaggag
    32881 cttcctgaca atccttgcga cactttcgaa aagcctaata cagatttgac tgtaatgaaa
    32941 tctgtgaatt cccgataaaa aataatattc aagtacaaca aaagcgtatt taaaatatca
    33001 atgatcaaaa gaatagctaa cgctggcttt ggctttttaa tatacaaaga aaggccaaaa
    33061 aacaataagg tagtcgcaat tgggttgatc cacaagataa agtactggat cgtaccttcc
    33121 acacctaatg aaaaatctgc atagtaggca atgatcgtct tcagccagaa aaagaaaaca
    33181 gctgtcaaaa cgattgctaa acgattttgg cacaattttt taagttgtgt cacgtatatc
    33241 acttctctct attcacattt tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa
    33301 agttgttatc ttaagatttt tttattcttt ctaaagatta ttccgtccac ttttcacagc
    33361 ataaaaccgc tgcgatgtaa gcgattaaac taatgaagag ataaaagtct tgtggtgacc
    33421 gcttcactcc acggtattca aaagccagtt tctcggtatt cgtacaaaaa aagaggctgt
    33481 gacaaaattc ttgtcgcagc tccttatttc ttgatgcagg acagcttttt cacaacttct
    33541 tccttaaacg gtgttctcct gccaactcct cggccacaag tcacaatatt tataaaacat
    33601 gagaagctgt tttttgaaat tgttccttgt ggctcagagt taaacggcag gctcaagacc
    33661 tcttgataca cggtgttcaa tcagctgacc ctcggtatta gattatgtca ggacgctaat
    33721 tttacaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    33781 aacgaaaaat ataaggagct attttttgaa attgctcctt gtggctcaga gttaaacggc
    33841 aggctcaaga cctcttgata cacggtgttc aatcagctga ccttcggtat tagctcaaaa
    33901 aacggcaaaa tatgaggaac tattttgccg tttgtttctc taatactcag gtcaaaacgc
    33961 tgatttcaca acctctgttt ttcattgtcc ataggattga tattgtcctt gtgatgactc
    34021 attcgtaata attgaggctt gattttcttc acttcccgtt atcgtttgtc cactgccaga
    34081 atctgaagga cttgttcctg tcgactcgtt tttttgagaa gagacagttg ttttcttgac
    34141 ttgttcattt gtctttttcc attccgctaa acgatcggcg accacttttt tgtctacttt
    34201 ttcagaataa gcggtgatca cttttccatt tttcaaacgg gctactttgt cgccatcatg
    34261 agtcaaaagc aaaacggtca aatgcttgtc tttaaattga tattgtctga gagaaatcgc
    34321 actaagtacg tcatcgatat taataaattg attttcttct tttccttttg gcaaataaat
    34381 gtccaattcg cctttttcgt aattggcagt tattttatac tccgcccacc aataagttga
    34441 gaattctttt ttgaaattat ctactgattc tttgcttgct acgatttttt ctgtttcttt
    34501 ctgagcaaga tctgctgtca tttttcctga aaatgctgcg gataaaataa gcagtaagaa
    34561 aaaaccgaat aaccaaaaga gaagaaacgg tctttttttc ctcatgcttt cctccactcc
    34621 tatttaattt tgactattct attatataca aaaaatgaaa tagaaagaca aaaaagttga
    34681 aaaagatcgt aaataattga acttcttttt cacaaaatgg taagaatgga gaatttcacg
    34741 catttgctct caactattct ttcttaagcc tcaaatccgt ttttatctga tacgtctttg
    34801 aaccaatgac ccgatttttt gattgttctt tttccatctt gtgctaagtc tactgaaata
    34861 aagccatatc gattcttata tgcattggtc cacgaccagt tatccataca tgtccacata
    34921 tgatagccta aacaattggc accttcttga atggcttgat ggacatattt cagatgagat
    34981 tggacaaatt cgatccgata atcatcgtgg atcatgccat cttctttgat aaatcgtgct
    35041 tcgttttcta cacccatacc gttttcagaa ataaagcagt cgatatttcc ataatgatct
    35101 cgtacattga tcaaaatatc atagattcct tgttcataga tctcccatcc gcgataagga
    35161 ttcattttac gaccaggcat ttcataattg tcgaaataat cctcaggcat tggaccgttt
    35221 ttagtatcga ttggtgtttc ttttgctttt acccggcgtg gctggtaata attgatacct
    35281 aaaagatcta ctgtattttc tttgataacc gtcaaatcct ctgcatggat cacaggcatg
    35341 agatcaagtt cttttactat ctcaatcaat tcacttggaa actcgccttt tacagctgga
    35401 tcaaggaatg aacgattgaa gaacgcatct gctatttgcg ctgctttaac atcttcaggg
    35461 tttgattcgt ctcttggata gcttggtgtc agattcaaga tgataccaat aacaccacct
    35521 tgctccattt catggtaaga tttgatggct aacgctgaag ccaacgcttc atgatagcca
    35581 acttggatcg cttttttcat atctgactct tctggataat gccatcgata taaataacct
    35641 gcttcgactg gaacgattgg ttcattatga gtaaaccatt tttttacttt cgaaccaaac
    35701 aattcaaaac aacttttggc atatttttca taggcttcaa ccgtttctcg gttcaaccat
    35761 ccgccttttt cttgcagata catcggcata tcgaaatgat acagattcac aaagggttcg
    35821 ataccgtttt caatcaactc atcaaaataa cgatgataga aacgaacggc ttcttcgttg
    35881 attgttccag attcatcagg tatcaaacgg ctccattgga tagatgttcg aaatgaatta
    35941 tgcccagtct gtttcatcaa ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt
    36001 ttttcaggtc ctacttgatc aaagaacttt tgcggttctt ttttgtacca ataatcaaaa
    36061 atgttctctc cttttccatc gcctgaaatc cttccttctg tttgtgggcc acttgctgca
    36121 gaaccccacc aaaaatttgc tggaaattga tattttttca tcaaagcacc tctttttctt
    36181 ttttagttcg ttttagttga ttccatgtat gatattttcc atcacaatca ctttttcatt
    36241 ttcttcctct tcttttttga ttcgttttaa cagctgttct gctgctcgtt ccccgatttt
    36301 atctgttggt ggcgtgatcg tgattttcgg aaggatactc agatcgtttt tatctaaata
    36361 atcatagtga ctgaacgcaa tatccttccc gattctgaca ccttcttgat tgaaacattc
    36421 gatggcacca atcgacatat tgtaattgct gacaaaaata gcagttgccc ctttttctaa
    36481 taatgctttt gccccgtaat agccgctttt tctagaatag tctcctgtaa agacctctaa
    36541 ttggatcgat ggatcatttt ggaccgcttc tttggctcct gctaaacgtt ctcttgctac
    36601 gtagtctgtt tgagtggcag caataaatcc gattttttta tgtccttgtt caccatgatg
    36661 agcgatcact tttgctacac tttctcgatc attcactaat attgtatcaa tacctgctgg
    36721 tccttctgga caattcaatg cgataactgg aatactcatt tttgtgagca tttcaaatcc
    36781 agaccactct tcttcggata agaagacaat cagcccatcg atcacatgct tcatcaggta
    36841 ttcaattttt tgttcaattt tttctggctg atcggcaaac ccgcttaata ataagctata
    36901 cccattttct tcagctactc tttcgatccc tgagacaact tccgacccaa aacggctgga
    36961 aatcccgttt atcaaaagtc caatagagaa gcttcgatta ttcttcaacc ctttagcaat
    37021 gatgttttct ttatagtcca acgctgaaat cgcttctgct atttttttca tgttcttttc
    37081 cttcagttgc tgaccattca agtaacggga aactgttccc actgaaacac cagccatctt
    37141 agccacatca cggatcgatg ccatacattc tcactcctgc cagttacatt tgatttctct
    37201 ttcttgttgt tcgattatga tgaccgcatt ctttgcatag tgttgcttgt catcaatatc
    37261 catataacta aagtcttcag tagagcaact tcctctcact attaattata cttaaacaag
    37321 tatttgaacc ggttcatttt taatatagca tttttttaaa agattgcaac acttttttaa
    37381 aacgctttct gaaagtggcg gattttttgc c
//
LOCUS       DAYYNG010000007        37201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000007 DAYYNG010000000
VERSION     DAYYNG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.007"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..476)
                     /locus_tag="ACHBFA_000295"
     CDS             complement(111..476)
                     /locus_tag="ACHBFA_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393822.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            684..1166
                     /locus_tag="ACHBFA_000296"
     CDS             684..1166
                     /locus_tag="ACHBFA_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393823.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1363..2253
                     /locus_tag="ACHBFA_000297"
     CDS             1363..2253
                     /locus_tag="ACHBFA_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM9393824.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2578..5049
                     /locus_tag="ACHBFA_000298"
     CDS             2578..5049
                     /locus_tag="ACHBFA_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9393825.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5152..6888)
                     /locus_tag="ACHBFA_000299"
     CDS             complement(5152..6888)
                     /locus_tag="ACHBFA_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9393826.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6885..7589)
                     /locus_tag="ACHBFA_000300"
     CDS             complement(6885..7589)
                     /locus_tag="ACHBFA_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9393827.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7720..8223
                     /locus_tag="ACHBFA_000301"
     CDS             7720..8223
                     /locus_tag="ACHBFA_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM9393828.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8183..8521
                     /locus_tag="ACHBFA_000302"
     CDS             8183..8521
                     /locus_tag="ACHBFA_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9393829.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8542..9960
                     /gene="ffh"
                     /locus_tag="ACHBFA_000303"
     CDS             8542..9960
                     /gene="ffh"
                     /locus_tag="ACHBFA_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9393830.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10072..10650
                     /locus_tag="ACHBFA_000304"
     CDS             10072..10650
                     /locus_tag="ACHBFA_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9393831.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10654..11175
                     /locus_tag="ACHBFA_000305"
     CDS             10654..11175
                     /locus_tag="ACHBFA_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM9393832.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11254..11808)
                     /locus_tag="ACHBFA_000306"
     CDS             complement(11254..11808)
                     /locus_tag="ACHBFA_000306"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9393833.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12061..12336
                     /gene="rpsP"
                     /locus_tag="ACHBFA_000307"
     CDS             12061..12336
                     /gene="rpsP"
                     /locus_tag="ACHBFA_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9393834.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12348..12599
                     /locus_tag="ACHBFA_000308"
     CDS             12348..12599
                     /locus_tag="ACHBFA_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM9393835.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12724..13515
                     /locus_tag="ACHBFA_000309"
     CDS             12724..13515
                     /locus_tag="ACHBFA_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393836.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13685..14206
                     /gene="rimM"
                     /locus_tag="ACHBFA_000310"
     CDS             13685..14206
                     /gene="rimM"
                     /locus_tag="ACHBFA_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9393837.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14196..14957
                     /gene="trmD"
                     /locus_tag="ACHBFA_000311"
     CDS             14196..14957
                     /gene="trmD"
                     /locus_tag="ACHBFA_000311"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9393838.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15093..15440
                     /gene="rplS"
                     /locus_tag="ACHBFA_000312"
     CDS             15093..15440
                     /gene="rplS"
                     /locus_tag="ACHBFA_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9393839.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15813..16475
                     /locus_tag="ACHBFA_000313"
     CDS             15813..16475
                     /locus_tag="ACHBFA_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393840.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            16554..19781
                     /gene="fss3"
                     /locus_tag="ACHBFA_000314"
     CDS             16554..19781
                     /gene="fss3"
                     /locus_tag="ACHBFA_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9393841.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            19895..20209
                     /locus_tag="ACHBFA_000315"
     CDS             19895..20209
                     /locus_tag="ACHBFA_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9393842.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            20222..20596
                     /locus_tag="ACHBFA_000316"
     CDS             20222..20596
                     /locus_tag="ACHBFA_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9393843.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            20606..20950
                     /locus_tag="ACHBFA_000317"
     CDS             20606..20950
                     /locus_tag="ACHBFA_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9393844.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            21021..22373
                     /locus_tag="ACHBFA_000318"
     CDS             21021..22373
                     /locus_tag="ACHBFA_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9393845.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            22433..23575
                     /locus_tag="ACHBFA_000319"
     CDS             22433..23575
                     /locus_tag="ACHBFA_000319"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM9393846.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            23600..23854
                     /locus_tag="ACHBFA_000320"
     CDS             23600..23854
                     /locus_tag="ACHBFA_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393847.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            24110..25294
                     /gene="mobT"
                     /locus_tag="ACHBFA_000321"
     CDS             24110..25294
                     /gene="mobT"
                     /locus_tag="ACHBFA_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9393848.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            25291..25428
                     /locus_tag="ACHBFA_000322"
     CDS             25291..25428
                     /locus_tag="ACHBFA_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9393849.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            26174..28084
                     /gene="ltrA"
                     /locus_tag="ACHBFA_000323"
     CDS             26174..28084
                     /gene="ltrA"
                     /locus_tag="ACHBFA_000323"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9393850.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            28188..28412
                     /locus_tag="ACHBFA_000324"
     CDS             28188..28412
                     /locus_tag="ACHBFA_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393851.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            28425..28925
                     /locus_tag="ACHBFA_000325"
     CDS             28425..28925
                     /locus_tag="ACHBFA_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9393852.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            29022..30869
                     /locus_tag="ACHBFA_000326"
     CDS             29022..30869
                     /locus_tag="ACHBFA_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM9393853.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            30872..31768
                     /locus_tag="ACHBFA_000327"
     CDS             30872..31768
                     /locus_tag="ACHBFA_000327"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9393854.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            31818..32207
                     /locus_tag="ACHBFA_000328"
     CDS             31818..32207
                     /locus_tag="ACHBFA_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9393855.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            32194..33693
                     /locus_tag="ACHBFA_000329"
                     /pseudo
     CDS             32194..33693
                     /locus_tag="ACHBFA_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            34441..36267
                     /gene="ltrA"
                     /locus_tag="ACHBFA_000330"
     CDS             34441..36267
                     /gene="ltrA"
                     /locus_tag="ACHBFA_000330"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9393856.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            36380..37201
                     /locus_tag="ACHBFA_000331"
                     /pseudo
     CDS             36380..37201
                     /locus_tag="ACHBFA_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT        12656 a         6326 c         7943 g        10276 t
ORIGIN      
        1 tttaagagta taaaagagaa aaaaagtaat aaatgtatac atgatataag aaaaacatat
       61 atgtataaaa agaagaaagt agcttctctt tgtacatata tggtttgttt ttatggatgc
      121 cgagcttttt tataagataa ggctaccgcc cataacgacc caaggatttg tggaactaag
      181 taaatacctg caagaagcaa gatcagctgg gtcggtttta aaggatgata gaagtaacgc
      241 cattcagtaa aattggcgca aaataaatag gaagtgacag tagatataat attggcaatg
      301 aataaagata taatacgcct gttcctttga aaataatatc cagcaaaaag agataaaaat
      361 atcaatgtga tgaacccgct tacggaatga taacgaaaat caagcagtaa ggcgaaatgc
      421 acataaggaa caaaagataa aagcaacgga aggtttttcc agcaagtttt tatcatcttt
      481 catcaccacc taacaagtgt tattattttg ttagttttat tgtaaattga atcctgcaat
      541 aatcaattac tttttcatta tttctagatt aaatgaataa actctcatgc cattatctct
      601 aaaaaaaaag tttttccccc taattttttt agatagaaaa ttgtatagta tgatgagaga
      661 aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat
      721 ttttttggtt ttttcctatc gttttgctac cttttacatg ggtaaatgct gttgtacaag
      781 gtgcggagat attaggcctg atccaaggaa gcggatttgt ggtttctagg aattttccag
      841 ctttgactgt tttctattta gtgataagtg taggtctctt attaccaaaa tctctgatca
      901 atcactacaa ttggcagctg ctttactttt cttgcttatt agtatttaca tctatgtttt
      961 ctttgctgcc tcgcttgatt atcggcccta atggtttgag cgagagtgga ttgaaggatt
     1021 cagcttattc ctccatgtat ttcttgacga tcaaaccatg gttctattta gtaattgcct
     1081 catttattct tgcaacattt ttttacttct ggacagaaat caggataaaa agtgaacgat
     1141 ctgtatctat aaaaaataac tattgatggt ggatcatcag taaaaaagca tatgagctgc
     1201 gtaaaatgca tatttctcat atgctttttt tattattatg taaagaatgc gaaaaacgtt
     1261 tacatgattt ttacataact taactatatt tttacagcaa ccatacgagg gatttacagt
     1321 cgactgataa actttagttg taaataagaa aggagcaata aaatgaaaaa agtattgttt
     1381 actctaggta tgatgggaat tcttttagcc ggatgcggct caggatcagg aaatacagat
     1441 ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta
     1501 ggttcgacag cattacagcc gttagtcgat gcagcacaag aaagctttgt acaagaaaat
     1561 ccaaattatc aaatctctgt acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa
     1621 gctggagcag ttactatcgg aaactctgat gtatttgcag aagaaagaga cggagttgat
     1681 gcttcaaaat tagtggatca taaagtagca gtggtcggga tggcgccaat cgtcaataaa
     1741 gatacagatg tcaaagatat caccaaacag gaattgattg atatctttac aggaaaaatc
     1801 actaactgga aggaagttgg cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac
     1861 ggcagcggta cccgcgcgac attcgaaaaa tggggactgg atggtgctac ccctgtgcag
     1921 tcccaagaac aagattcatc aggaacagtt cggcagctgg taagtcaaac accaggagca
     1981 atcagctatt tagctttctc atatctcgat gattccacac aagcactaag tatcgacggt
     2041 gtagaaccaa aagaagagaa tgtagcagat aacagctggg gaatctggtc atatgaacat
     2101 atgtacacga acggaaaacc ttcccctgaa gtccaaaaat tcttagacta catgatgaca
     2161 gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt taccaatcac aatgatggaa
     2221 gtagaacgtg atcacgaagg aaatatcaaa taactagcag ataaacatgc cacggtagtc
     2281 gttactatta aggaaaaatc aactatgttt cccacatata catttttcct ttaattgtga
     2341 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattaaac
     2401 accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtagctcttt ttttgctata
     2461 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
     2521 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
     2581 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
     2641 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
     2701 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
     2761 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     2821 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     2881 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     2941 acagcaaaag tcaatgtcat acgggacggg atagaacaaa caatagatat agaagacctt
     3001 gtaatagatg acattgttat tttagagact ggacatcaaa ttccagcaga tgcagaggtc
     3061 gtggaaggaa aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     3121 aaagaaatag gcgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     3181 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     3241 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     3301 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     3361 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     3421 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     3481 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     3541 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt gatcgtctct
     3601 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     3661 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     3721 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     3781 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     3841 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     3901 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     3961 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     4021 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     4081 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     4141 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     4201 aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac aggtgatggc
     4261 gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat
     4321 gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca
     4381 gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt
     4441 ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc
     4501 tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct
     4561 ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg
     4621 atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc
     4681 tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg
     4741 attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt
     4801 cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg
     4861 ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg
     4921 gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat
     4981 gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat atttaaacga
     5041 gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt
     5101 cgaagcagga tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt
     5161 cggaatggta acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc
     5221 attaaggagt tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg
     5281 tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat
     5341 gtcgatacct ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg
     5401 gataataatc tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat
     5461 aattggataa aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat
     5521 cgtcaaccgt ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg
     5581 ttctgtataa attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat
     5641 ttcccgaatc agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt
     5701 cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga
     5761 agtcacagga gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc
     5821 taaacgtcgc actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga
     5881 attagtaaag tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc
     5941 ctgatggatc agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc
     6001 ttgaatgact tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg
     6061 catctttgga ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat
     6121 catcagttcg ttcagtaacg tatataattg ctcttctgtg gtagtacacg cccgatacat
     6181 cttactcatt tgttcgctca acgcattaat cgtctgatac aattcttccc attgatcggg
     6241 tgtttgcatg attacttccg cctgattagg attttgcacc atttttttta gtacgggcag
     6301 cacggttttc aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac
     6361 aaggaagaaa gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa
     6421 aaaaccgctt gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac
     6481 tggtaatgcg acatataaaa gatcctcatt caaagtagta cttttgcgca aagcgctacc
     6541 taggttccca ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa
     6601 tgtttcattg tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc
     6661 gttcgactga tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc
     6721 gacatctaat tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc
     6781 cacgatctgc tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac
     6841 taaaagaata agaaagaaga gacgttcata aatttttttc catttcatcg ttttggttcc
     6901 tgaaacttat agccaaatcc tctaatagtc accaagtact ttggatgttt tggatctcgc
     6961 tcgatttttt ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag
     7021 tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc
     7081 atgaaataaa caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg
     7141 gtcacttgat aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca
     7201 gcttcatctt ttgattgctt acgaggttca atccgtctga atattgcttt catacgggct
     7261 aatacttcgc gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca
     7321 atgatccgat ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt
     7381 tcttgacgca gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa
     7441 atgatgaaat caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca
     7501 ctggttacct tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt
     7561 tcatcatcaa caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt
     7621 ttatatttta gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa
     7681 taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt
     7741 ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa
     7801 ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt
     7861 cctagagatc ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca
     7921 aaagtatgga cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa
     7981 gcctatcaaa aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct
     8041 tcatgcggaa cccattcgat ttatgacgga agtttttccg gaataaaaaa aaatggtgcc
     8101 ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg
     8161 ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt
     8221 tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc
     8281 agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata
     8341 tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac tccatctttt
     8401 ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa
     8461 gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata
     8521 aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg
     8581 atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg
     8641 gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact
     8701 aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa
     8761 caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag
     8821 ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt
     8881 aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg
     8941 ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt
     9001 caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc
     9061 cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca
     9121 ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg
     9181 caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta
     9241 gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg
     9301 gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc
     9361 atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc
     9421 cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactacgac
     9481 gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac
     9541 tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg
     9601 atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt
     9661 gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg
     9721 ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg
     9781 aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg
     9841 gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc
     9901 aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa
     9961 gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag
    10021 caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt
    10081 ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct
    10141 gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg
    10201 agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat
    10261 atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga
    10321 attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa
    10381 attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc
    10441 gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa
    10501 ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct
    10561 tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag
    10621 gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct
    10681 ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact
    10741 ccaacaggta agcaaactac ttatgaagct ttttatgaaa caatcgatac gaccatcatg
    10801 ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag
    10861 aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta
    10921 aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg
    10981 gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg
    11041 tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca
    11101 tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat
    11161 tatgtacgaa agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt
    11221 acatagttgc cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc
    11281 tagatacgca tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt
    11341 ttcatcaatt agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac
    11401 attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc
    11461 tggtgatgca gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct
    11521 tgagcctaca tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt
    11581 aacgaaaacg acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg
    11641 agcctgttct ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa
    11701 tacactttcg tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta
    11761 agaatctttt cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct
    11821 ccttcaaact atctataata atcttacaaa agataagtaa ctggagcaaa taaaaacact
    11881 tactgacaaa agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa
    11941 tcttctattt tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag
    12001 ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata
    12061 atggcagtta aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc
    12121 gtcgtagctg attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac
    12181 aatcctttga aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta
    12241 tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg
    12301 caaaaacacc acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa
    12361 gcgacttagt cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag
    12421 acgtcgtaga gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg
    12481 gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg
    12541 ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa
    12601 taaacgtgct gtactcgaat tttcgagtgc agcttttttg ttttttttat taattgaaga
    12661 aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag
    12721 ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg
    12781 gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt
    12841 ccagaaaaaa gtagtaccaa aacggcttcc accactgaac aaacaatggc tacctcaaat
    12901 aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct
    12961 ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt
    13021 tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat
    13081 caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca
    13141 atccaagttt tgcagccatt tgagatcgac acaaatggtc agcaagtgga cttaggttct
    13201 cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga
    13261 aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct
    13321 aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa
    13381 attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg
    13441 aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct
    13501 atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag
    13561 gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga
    13621 aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata
    13681 taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga
    13741 agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgct
    13801 aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca
    13861 taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata
    13921 tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt
    13981 ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt
    14041 aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc
    14101 tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt
    14161 ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat
    14221 ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag
    14281 gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag
    14341 tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg
    14401 ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg
    14461 acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact
    14521 tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag
    14581 atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga
    14641 tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg
    14701 attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag
    14761 ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat
    14821 taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat
    14881 ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag
    14941 cagatgaagc caagtgactt tttctaaaat cttctttaca gtaggttgat aatatgttag
    15001 tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctgtg agaaaagctc
    15061 ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa
    15121 aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg
    15181 caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac
    15241 gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg
    15301 ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg
    15361 gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta
    15421 tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat ttgtgaaaag
    15481 tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc atttttggct
    15541 gttttggacc atttttcaat catattccct taaaaattgt cctagattgt ccgacattga
    15601 ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca aatataatac
    15661 gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt ctcaaagggt
    15721 cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga tgtgataggc
    15781 ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga cgtatcattt
    15841 ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc gaaaaacacg
    15901 gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat tctgcaccaa
    15961 gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt agtataggac
    16021 gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta ttggaaatag
    16081 tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa caaaaatcag
    16141 tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat atacgtccac
    16201 tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg ttagaactaa
    16261 aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag tttcgaaaac
    16321 tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg aacaaaaatt
    16381 gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt tataagcaga
    16441 gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca gacgttcaaa
    16501 attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat tttatgaaaa
    16561 agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc caaacaattg
    16621 gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca caaacggtaa
    16681 ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc gatggtcgtc
    16741 ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag caagatattt
    16801 tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat gagaaaaatc
    16861 cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag gaagctggta
    16921 cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt aatggctaca
    16981 cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc attgagtcaa
    17041 aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac tccacagtga
    17101 aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat ttatcggagt
    17161 ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc aatcaactag
    17221 tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag aagtcagcta
    17281 atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg gcgggggcaa
    17341 ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt tctttgaaga
    17401 ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc catttagact
    17461 ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt gcgacattgg
    17521 atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca gctccttata
    17581 cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt tctgtgactt
    17641 caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg gaaacaggaa
    17701 ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt cgcaaagaca
    17761 gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat gcggaaacac
    17821 caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa accaaagcta
    17881 gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat gtcaatcaaa
    17941 ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact ggtgaaacca
    18001 ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct gagtttaaag
    18061 gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg agtgaggctt
    18121 ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact ttgactttag
    18181 atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac tggcaagaaa
    18241 ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc atcaaaaagg
    18301 tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa gaacaagtca
    18361 ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact gccgaaactg
    18421 gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag attacagatg
    18481 ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc tatggcaagt
    18541 ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca gaaggatttc
    18601 aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg gattacacta
    18661 agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt aaaacggtga
    18721 ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat cgtttgatgc
    18781 tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg aaagacggag
    18841 gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc tataacttgc
    18901 atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa gcgacaaaag
    18961 ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca accgcaacgt
    19021 tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc gcagaacaag
    19081 ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa ccctatgaag
    19141 caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa actgtcaatt
    19201 gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat ggttcgcaaa
    19261 cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc catgatgtac
    19321 tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg gataatacga
    19381 ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa gaattcacta
    19441 aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca agttttacct
    19501 tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat gaggatctaa
    19561 aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt gcaccagaac
    19621 aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca gtaaagacat
    19681 tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt accttgatct
    19741 ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg atgaaacaac
    19801 taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc gctatttttg
    19861 tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg ttgtgccaga
    19921 tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt taactgaagg
    19981 atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca gtaagcaacg
    20041 agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt ttgatcaaga
    20101 tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga ttgagaatcg
    20161 tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg aggaaaagaa
    20221 gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga aattacgatt
    20281 ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta gtaacgaggt
    20341 aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc aagttagtat
    20401 cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt tggtcaatcc
    20461 aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca attggttcat
    20521 taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta caaaccaaaa
    20581 tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt tagattgtct
    20641 cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat taacctatga
    20701 tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg atgccgaaga
    20761 agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg ttatcgaagt
    20821 aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg tggaaatgcc
    20881 ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc ctatagcagt
    20941 aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc ttttttcgat
    21001 tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga gatcagcact
    21061 tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg atgagcgtct
    21121 tttattttca agagcttaga caatttgata tttctaagct atcactttct actatagaaa
    21181 tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt tcaggaagca
    21241 cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg catcgacaaa
    21301 agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact cagagtgaag
    21361 gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac ttccccaaaa
    21421 tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca ctgggaaaat
    21481 atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat tgtgaactag
    21541 tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat ataattgcca
    21601 atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg atgaaaaatc
    21661 aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca ggtggtggga
    21721 agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc gagctttttg
    21781 ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct catgtctatt
    21841 cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg atgactcgta
    21901 gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg tttcttggac
    21961 ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg ttaacgacga
    22021 aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt cgtcaatctg
    22081 gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga gacgggattc
    22141 gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga tattccatga
    22201 tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga ggctatgtag
    22261 atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa ggatatgatt
    22321 tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga taaaataata
    22381 gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat ttatgacatt
    22441 tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag cgggtcacca
    22501 gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg caatccatga
    22561 tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt ttattcaatc
    22621 tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt caattgcggg
    22681 aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg ctcggttcgc
    22741 cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac tcaatcacga
    22801 aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt atgatgtgga
    22861 atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga tcttccttat
    22921 cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat cgtctccaac
    22981 atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag gaatgggggg
    23041 acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag gaatcaaaat
    23101 cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa attttgaaca
    23161 aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga aacaaggcgg
    23221 aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc cagggaacag
    23281 tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa aacaaagtgt
    23341 gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc aaattcgaaa
    23401 gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt ttgatcgagc
    23461 ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg taacagttcc
    23521 ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt tatgatgagt
    23581 tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat tggaagaaga
    23641 actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg atgatccgta
    23701 tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca gtgaattgga
    23761 acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg agataaaagc
    23821 agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac tgataggcac
    23881 tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt ttatggacgg
    23941 cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa aaacacaaaa
    24001 aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca atttgtgtag
    24061 atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact tggcacaaag
    24121 gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt cacaaaataa
    24181 attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa atggtggcgt
    24241 aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt tcaatcccga
    24301 gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa cggatgcgag
    24361 gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg aagattacgc
    24421 cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt tgtcacatga
    24481 agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt ttgaaacttt
    24541 tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa aagccggtgg
    24601 cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag atattcctga
    24661 tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct tcaaaagcta
    24721 tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata ctttgtatat
    24781 tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg agcaatatat
    24841 caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa tccgactgaa
    24901 aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta ttgaaagtac
    24961 cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag gcaaaaggcg
    25021 aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc agaaggaaat
    25081 tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact ggcttggacg
    25141 acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc agacaacgta
    25201 cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga ttttggagca
    25261 acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat ggaaattgtg
    25321 aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat gtgcttgatg
    25381 catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg aaatgaattt
    25441 cggtcaaaat ttgtgtgcga ctcgttcctt agtgaaaagc taaggcacta ataaaagaac
    25501 gaaaaagttt ggttattagg agaactgata atctgacagg tggaatgatg aagtaacgct
    25561 ctgaaacgcc tgccctgagc ctccgacaag cattgtatga ggaaactcag aatgtttgaa
    25621 gctcggtaaa gacggctgaa agataccgta atagttgatg aacatatatc ttctatcgaa
    25681 aactatccag aggtgggtag cgtatccaac aggtcggggg tctataaaat atctatggtt
    25741 agaatgttct ataaggaact gacgaaacag cgaatgtacg ggtctaaatt tgaactttgt
    25801 agaaatgcaa aggtacgaga aatcgtagct ggtgtggcaa agtaagaata taggttatga
    25861 aatgccatat aacgttacag gcggtatcaa gccagcaggc ttatagctag tgacctaagg
    25921 atatatatgt atagataaga ttatcggaac gaggaaaggt attgggttct catcaagaga
    25981 ataaactgac gaagaacaat aagcagttga actaatgctg aaaagcagag ggcgaaccta
    26041 agaaattctt gtaatgagaa tggaggaatg ccctcaagtc gtgtatatat tagaaaagta
    26101 ttatgttgtt tcaataagga ttacgagtaa gataaaaaag ctcactcaat ttcaagtaaa
    26161 ggagtgaaac cttatgccta aaaataaaaa tgaaacattg tgtgtagaag atttaagaca
    26221 cgctgaatat tacgaaatgc aaaacacctt tgatgattta tatgccaaaa gtaagaatgg
    26281 agatattttc actcatctta tggatattat cctatcgaga gaaaatatat tactggcata
    26341 tcgaaatatc aaagcaaatg caggcagtaa aacagcagga acagacggta caataattaa
    26401 agatattggc aaattgccag cagaaacagt tgtcaaaaaa gtaagataca ttgttgcagg
    26461 cagtccacat gggtatcgac ctaagccagt gaggcgaaaa gaaataccaa aaccaaatgg
    26521 taaaaccaga cctctaggta ttccttgtat gtgggacagg cttatacaac aatgtataaa
    26581 acaagtatta gaaccaatct gcgaagctaa atttagtgaa aacagctatg gttttagacc
    26641 taataggtca gttgaaaatg caataaaagc aacctataac cgcctacaaa tcagccagct
    26701 acattatgtg attgaatttg atattaaggg tttctttgac aacgtgaacc attccaagct
    26761 gataaaacag atatgggcta tgggaatacg ggacaagcac ttgatattca ttttaaaaag
    26821 gatattaaaa gctccaatca aaatgactaa cggtacaata acatatccag agaaaggaac
    26881 tccgcaaggg ggaataatat ccccactact agcaaacatt gttcttaatg agttagacca
    26941 ttgggtagaa agccaatggc aggaaaatcc tgttacaaaa aactatgtgg ttcatatcaa
    27001 taaaagcggt agcccatgta aaagcaacgc ttacaaagaa atgaagaaaa caaagttaaa
    27061 ggaaatgtat atggttcgtt atgcagatga ttttagagtt ttctgccgct acaaggaaag
    27121 tgctgaaaaa gccaaaatag caatcactca atggattgaa caacgattaa aattggaagt
    27181 atcacaagaa aaaacaagaa ttgtaaatgt tagaaaaaga tactccgact ttcttggttt
    27241 caagattaaa atgataccga gaagaaagaa gttagttgtt aaatcacata taagtgataa
    27301 acaattcaaa aaccaaaaag acaagctggt atcacaggca aagaaaattg cttctccctc
    27361 aaaagggaaa acggaattag aagaaacaag actttataat tcaatggtat taggtatgca
    27421 aaattattat cagattgcaa cgaatgtaaa tctggattgt atgaaactga atagagctgt
    27481 aatggctatc ttcactaaca gattaggtac aaacaaaagg ggcagattga gaaaaacagg
    27541 cagacaactt acgacaaagg aggcaacaag atatggtaaa tctaaaatgc ttcggtatgt
    27601 tgctggcgag ggcgaaccta ttttccctgt tggatatatc caacacagaa aaccaatggc
    27661 aaaggtgtat ttagcgaatt gctacactct agagggaaga aaatttatcc acaccaatct
    27721 aagtgttgat aaattactga tgtaccagtt aatgaaatta tctttggaaa atcgcacaat
    27781 agaatacgca gacaatcgaa tttcgctatt ttctgctcaa catggaaaat gtgctataac
    27841 tctggaagaa tttcaacaag ctaatgaaat acattgtcat catattgtac caactggagc
    27901 tggtggaaat gacgattata aaaatcttat tttggtaaaa gaggcggtac accgtctaat
    27961 acacgctaaa acagaagaaa caatacaaaa atatatagct ctattgaagc taaataaagt
    28021 ccagctcata agactaaata aatatagagt attagctggc aatcaagaat taaacttgat
    28081 ataggtctac tacataaata cttttcaata taaacacgat ggaacgcgga gtgcggtgaa
    28141 agtcgcacgc tccgtgtgaa tggggggaaa agtttgagat atttataata tcaggaactt
    28201 acctatccat atatcaatgg tttttaacca atgctcaatc tttagtctta ctagcgattg
    28261 tggtcattgg tttgttttta ggatttaaac gcgagttttc caaactgatt ggcttcttgg
    28321 tgattgcttt gattgcggta ggtctggtct ttaatgcttc tggtgtgaag gatgtcttac
    28381 tcaatttgtt taaccgaatt gtcggtgcct gagggggaat cacaatggaa cagatacgtg
    28441 tttatattgc gaaccttgga aaatacaatg aaggtgagtt ggtgggggca tggtttaccc
    28501 cacccgtcga ttttgatgaa gtgaaagaac ggattggatt aaatgacgaa tatgaagagt
    28561 acgcaattca tgattatgaa ctgccttttg agattgatga gtatacacca attgaggaaa
    28621 ttaatcggtt gtgtggttta gcggaagagc ttaaaggaac gccaattggt gaggtcgctt
    28681 cagagattca acatgccttc ttcaattctt tcgaggaaat ggtagagcat gtggatgata
    28741 tcatctatta tcccgattgt aatgacatga gtgatgtagc gtattacttg attgtagaag
    28801 ctggtgattt aggcgaagta ccagaacact tgaagacgta tattgatttt gatgcgtgtg
    28861 gtcgagactt ggagattggc gggaattatt tggttacgag tcgaggggtt tttgaactaa
    28921 tgtaaatcat ttgttagaat tgaccagaaa tttctatcgt acttttactt ttctatttga
    28981 tatactgaaa tagaaaatat ttgttgatag gggtgttact aatggttggc aataagtggt
    29041 acaaatgtga tcttcatatt cattcaactg cgagtcagtg ctttcaggat aaaaaggtta
    29101 ctgctaagga atgggtagaa gaatgtttaa acaaaggact cgattgtgtt gcattaactg
    29161 atcacaatac aggggaaaat attgatgaat ataagaaagt ggctatcgaa aatggcttag
    29221 atttttttcc tggagtggaa ttaacgtgtg gagaaaatgg aactcattta ctagtcctat
    29281 ttgataaaag tgcatgtaca aatgatatct ctgatttctt aaccaagatg ggtataaaaa
    29341 gagaggattg ggccagcgct gcagcttgct caatgaaatc agttttagaa gttattgatt
    29401 ttgctgagca cgagggcttg ctcgttattc ctgcacatat agatgagttt aatggaattt
    29461 cttcagcaaa atttggaaat ctaggaaaga tatttgaatc agaaaatata tatgcagtac
    29521 agacggttca aaaagaattt tttgaaaatc gctcacaaat gattccctca tctaagcgtg
    29581 acactatcta tgatagtgtg aatgccagat atgatggtag agttggaaaa gatactttgg
    29641 aatcttggta taaatcagtt gttgaggcag aaaaaaataa tatgacattt cttagctttt
    29701 ctgataaccc acactcgaaa ggaaattcaa aacatggact ttggggaata ggcacaagat
    29761 attcctatat aaaaatgaag gacgaacctg acttgggatc acttcgcgat gctttaatgc
    29821 taggcgaaac tcgagttcat tctgattttt ctaatttctc cattaatgaa aatgaagttt
    29881 tattggaaaa attaagcttt tctgggacca ctcttagcac aaaagaagtt gtagtcgaat
    29941 tcagcgataa tttaacttca attattggag gaagaggaac aggcaaatct tgtattacaa
    30001 gattcttggt ctatgtcctt ggaaaggaag cagaacttga tcaattttct gaaatacaaa
    30061 gtgattatca gaactttgca caaattgaac ataatggctc tggaatattt ttgaaggata
    30121 caattgtgaa actgaatatt ttctataagg ggacaaaata tcaaataata agaacacagg
    30181 atagacattc tatttatgaa tttacacaag agaatggtct ggttgaaaaa gaaacagaac
    30241 ggttgagaat gatttccgat aaggtgagta tatactctca aaaacaaatt tatgaaattt
    30301 caaaaaatca aactagtata ctagaattac tagatggata taactctgat ttgatttcag
    30361 agtataaaaa tgagattgaa acatgtgtaa acgaaataag aaaattaaat tgggatattg
    30421 tttctgtgaa aaaagaaata caagataagg cgaaggtaga gcttgaaatt gaagatttaa
    30481 aaaaacaagt agaaaagcta tctcataaga gttataaaga tgtttatgat gagtattcaa
    30541 aagagacaga tatttatagg gaactgaaac gtgatgtgga ggcacttaag gaaattccca
    30601 aaaatctaac tgatagtgtt gacaaaattg actttggcaa tggtataaaa gttacagacg
    30661 aaattgatga acaccgcgga gaattgatta agaatttagt gaccaatcgg gctaagattc
    30721 aaacaataat taatgaaatg tcagatgaaa ttgatagtta tagagcagag ctgaataaat
    30781 cagattggaa agttaactat aatgaggttt caaagaggta tcaaagaggt atcaaaaact
    30841 tagtgaagag ttatctcaag atgaattaaa aatgttacaa aaccttgatt ctttatatca
    30901 atctttatca aaaactgaag agcgattgga agaaattatt caacaagaaa taaagcttaa
    30961 agattaccaa gttgaggttg actcagcaat gtcaagaatg aataaatcat atcaaaattt
    31021 ctattctgag agaagtagtt cctcgcgcga aattttttct gatattacag gaatagaggc
    31081 tactattcaa ccaaataggg atttcaataa ttatgttaaa gagctcagag cttgtattaa
    31141 taaggaggac acttttgaca gtgagtttgg atctcttatt gataagctga caaaaaattc
    31201 cattactaat caagatatat ataatgacat acttgatata agagttagtg gggaaagtag
    31261 tattttttca gataaaaaat taatcaattc tataagaaaa ttgaatgatg cacaagttct
    31321 tgaattacaa acccttaagc caaatgattt agtagttatc cgacttgaac taaatggaaa
    31381 aaaagttagt ttatcgaatg ctagtgctgg tcaaaagaca tctgcaattc tttctatgat
    31441 tttggcgtat ggaaattcac cactcatatt ggatcagcct gaagatgatt tggatagtca
    31501 attaattaat aacttaatcg taagaagtat cattacaaaa aaggaaaaca gacaaatttt
    31561 aattgtaact cataatgcaa atatcccagt taatggtgat tcagaatggt tgattagcat
    31621 gggagattca aaagaaatat cggctgatca gtgcgggtcg gtagatgaac ccaatatcaa
    31681 gccaagaata tgtactgtga tggaaggtgg cgaggatgct tttacaaata gagcgaaacg
    31741 ctatggtttt aaacaaattg tagaataaca ctagagggtg agacatatta ttatgtccgc
    31801 cctttttagg aggaaaaatg aaaaaaataa aaagctatac cagtatctgg gcagttgaaa
    31861 aagtcattta cgcaatcaat gattttcagt tgccttttcc tgtgactttt aaccaaatgg
    31921 cttggttcgt tttatcttta ctgtttgtca tcgtatttgc ccatgtgcct ccactatcta
    31981 tgatagagga ggtcttttta aaatacttag gcataccggt ggctgtcact tggtttatgt
    32041 cgcaaaagac gtttgatggc aagaaaccac tgggattttt acgatccttt atcagctatc
    32101 acttgcgatt taaggtgact tttgcaggaa agaaagtcaa agaacagaag aaacgatggg
    32161 atgaacccag tactcttgta aggagtgtga actatgtatc cgattaagta tattgaaaat
    32221 aatctcgtct ttaaccaaga aggcgagtgt tttgcctact atgaattagt gccttacaat
    32281 tactcctttt tgtcacccga acagaaattt caagtgcatg ataactttcg ccaactaatt
    32341 gcgcaaaatc gtgaaggcaa gatccatgcg ttacaaattg ccacagaaag tagtattcgc
    32401 gccacacaag aacgatcaaa aaaagaaatt accggtagac tcaaagaagt agcgaaacaa
    32461 cgaattgata tacagacaga tgcattagta tccatgattg gcgatagtca gattgattat
    32521 cgctttttta ttggctttaa actaattgcg acagatgaag aagtcaatct aaagagtctc
    32581 aagaaatctt tcttttctgg gcttcaagaa tttgtctatg gagtgaatca tcatttgatg
    32641 ggcgattttg tttccttatc caatgaagaa attcgctgct attctaagct tgaaaaatta
    32701 atggaaagca aattggctcg aagatttaaa gtcaggcgag tcacaccgag tgacctgacg
    32761 tatttgattg aacatattta tggcgaaaaa gggataccgt ttgaagaata cgaatttcag
    32821 ctaccaaaga aaaagctaaa atcagaaaca ctggtgaaac gttatgactt attacgtcca
    32881 aatcgttgtt taattgaaga aaaaccaagg tgcttacgga tggaacatga gaatcatgag
    32941 tcgtacgtag cgtatttaac aattaataca attgtggggg agatggaatt tccttcctca
    33001 gaacttttct attaccagca acaacaattt acattcccaa tcgacacaag tatgaatgta
    33061 gagattgtga cgaataaaaa ggctttagct accgttcgta ataagaaaaa agaactgaaa
    33121 gatttagaca atcatgctta ccaatctgac aatgaaacga attccaatgt cttagatgct
    33181 ttggattccg tggatgaatt ggaaaccacc ttggatcagt caaaagaatc tatgtacaaa
    33241 ctgagttatg tggttcgagt gagtgccgaa tcagtggatg aactaaagcg acgctgtgat
    33301 gaagtgctcg atttttatga tgataccaat gtaaaactag tccggccctt tggcgatatg
    33361 atggggctac atgaagaatt cttaccctta agtaaacgat atatgaacga ctatattcag
    33421 tatgttacgt ctgatttttt ggcggggctt ggttttgggg ctactcagat gctaggagaa
    33481 ttagaaggga tttactttgg ctacaatgtg gatacaggtc gtaatgttta cctgaaaccg
    33541 gcattggctt cacaaggagt caagggttca gtaaccaatg cgctggccgc cgctttcctg
    33601 ggttctcttg gtggaggtaa atcctttagt aacaatctct tagtctacta tgctgttttg
    33661 ttcggtggtc aagctgtgat tgtcgatcca aaagggggtg cgaaacgttc ctacttgaaa
    33721 agagtaggca cagcacttca ttaaatcaat atttaatgtg tgttggagaa ctaaacacac
    33781 gaatagtcga atgacggggt aacgccctga aaggctactt taatactctg actggcagaa
    33841 acttgagtaa acaaggctct gtcagaagct cggtgaagtc ggcagacaga caccgtaaca
    33901 gataatgctg acgaaagatt cccagaggtg ggcgttgtgt cctatatgcc ggaagtctat
    33961 aaaatatcta tggttagaat gtagatgaga ggtctactga cgaatccttg aatgtacagg
    34021 tctatatcgg ggctggatag aaatgtccag tgacacaaac agtgtcgcta ctgtgggtga
    34081 gtaagactcc gcatttatga aaatccatat aacgttaaag gcgttatcaa ggtagcaggc
    34141 ttatagagga aacctaagga tatatgtaca gatagtgtgt ttggaacgtt gaaagctgac
    34201 aacatggaga ttttactgtc tgtgaagtgt tcacatcaga aagttattgt gagattagca
    34261 atgatataag gtgtgttaat gtcagtgaga gtggtggcac agtacctatg aaaccgtgat
    34321 aacaagcggt ggagggatag ccactagtca tatgaagaat agaattacta agaaaaatgg
    34381 tatgggttcg agtaagacta agagatgtac ttgctcctgc aaacaaggag ggtacagacc
    34441 atgctaaaga aaaacaagct ccgctataac gagtattatg atatgcaaca tatttatgac
    34501 gagttatacg cacagagcaa gaacggtaat aacttttata agttacttga aattattggt
    34561 tcggaacaaa atatttgttt agcttaccgt aatctgaaat caaacagtgg cagtaaaacg
    34621 gctggaacgg atggaatgac gatagacgat ataaaacaac tcagcaatgc tgagattgtt
    34681 gctaccgttc gggaaagcct tagcaactac cgccccaaat cagtcagacg tgttttcatt
    34741 ccaaaagctg ggagcgataa aatgcgtcca ttggggattc cgtgtatctg ggacaggctg
    34801 gtacaacaat gtatcctgca agtcctagag ccgatatgcg aaccaaagtt ccataatcat
    34861 tcctatggat tccgtgcgaa tcgaagtgcc caccatgcag tcagcagagt aacgacactg
    34921 ataaatttat caaaatatca ttactgtgtt gacgtagaca ttaaaggatt tttcgataat
    34981 gtcaatcatg gaaaactatt aaaacaaatc tggacacttg gtattcggga taaaagattg
    35041 atttgcatta tcagcaaaat gctgaaagcc gaaattgacg gcgagggtgt cccagaaaaa
    35101 ggaacgccac aaggcggtct gttaagtcca ctgctctccc tcatagtcct taatgaatta
    35161 gactggtggg taagcagtca gtgggaaaca ttccagccga agaaccgcag taaaaatggg
    35221 tggcttcaat acgcaaagaa atataccaaa ctgaaaagcg gttttattgt acgttacgct
    35281 gatgatttta aaatcatgtg ttcaacctat ggagaagcac aaagatttta ccacagtacc
    35341 gtagattttc ttaataaaag gctgaaatta gaaatcagtc cagagaaatc aaaggtagta
    35401 aatctgaaga aaaattcatc agattttctg gggttcaaaa tcaaagtcat tccaaaagga
    35461 agaacgaaac acggttatgt tgccaaaaca gatatgaacc aaaaagcatt aaagaaagcg
    35521 aaaacaaatc taaagttaaa ggtaaaagat atagccagac atacgactgg cttcaatatc
    35581 agcagatata atctgacagt tatcggtatg cagaattact actgcatagc aactaacgta
    35641 tataacaacc tgacagaagt aagttacgct ctcttaccga ccatacgaat ccgtttaagg
    35701 aacattgcaa aatcagttcc atttgaaagt acaagttcag aatttcagag caggacaaaa
    35761 ggaatcagac caaaaacaaa aatagtgatg attgcggata atccgctatt gccgattcaa
    35821 ggagtacaac acaaaaatcc tatgaatttc tcgcaagata tttgtaattt tacaaagcaa
    35881 gggagaaaca aggtgcatga ggacgttgta gttgtaacaa aagaagaaat ccgagcgttg
    35941 ttggaaaatg aaaaccctgc cgatagcgta gagtttaacg acaatcgtat ttcggcatat
    36001 atcgcacaac aaggcaactg ctatgtaatg aatcgtcgag gaactccgtc cactctgata
    36061 tgtattcaca aatcagacaa aggagataac cttgacagat attctaatct ggcatttgtg
    36121 gaaattccca ttgcaaaggc aatcctaaca gaatcagcag acgaagcaaa atcactgtta
    36181 aaaggttatg ttcttaatag tcagcagaag aaaaaactga acagaatacg gacgaattac
    36241 ggataccaga caataactgg gaaataactt ataaatctat ttggatatga tggaacgccg
    36301 tatgaagcga aagtttcatg tacggtgtga agcgggggaa aacttggaga taacatcaaa
    36361 aagttaccta tcgctatagc gaggagggtg gaaagaaacc ttgccagaaa ttgcggatga
    36421 aatcagtatc ctcaacttga cgagtaagtc agaaaatcaa ggtctgcttg atccttatgt
    36481 gattatgaag aaaaagaaag actcggaaag tttagcaatt gatattctga cttttttaac
    36541 aggaatttca agtcgagacg gggagaagtt tcctgtgtta cgtaaagcca ttcgaacggt
    36601 gactcaaagt gatcaacggg ggttattaca agttattaac gaattgcgaa aagacgggtc
    36661 accaatcgcg gaaaatatcg ccgatcatat cgagtccttt accgattatg attttgctca
    36721 tttgcttttc tcagacggct cgattaccca atcgattagt ttagataaac agctaaatat
    36781 cattcaagtg gcggatttgg ttttaccgga tgcagaaacg acttttgaag aatataccac
    36841 catggaattg cttagtgttt cgatgctaat cgtcatttcc acttttgcct tggactttat
    36901 ccatagtaac cgagaaatct tcaaaattgt cgatttggat gaggcttgga gttttcttca
    36961 agtggctcaa ggaaaaacgt tatcgaataa gcttgttcgt gctggtcggg ccatgaatgc
    37021 tggggtctat ttcgtcactc aaaatagcga cgatttatta gatgaacgtt taaaaaataa
    37081 tatcgggttg aagtttgctt ttcgcagtac cgatattcat gaaatcaaga aaaccttgga
    37141 attctttggt ttggatcagg aggatcaaag caatcaaaaa cgcctgcgag atttggaaaa
    37201 c
//
LOCUS       DAYYNG010000008        36568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000008 DAYYNG010000000
VERSION     DAYYNG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.008"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHBFA_000332"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHBFA_000332"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9393857.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHBFA_000333"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHBFA_000333"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9393858.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHBFA_000334"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHBFA_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9393859.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHBFA_000335"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHBFA_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9393860.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHBFA_000336"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHBFA_000336"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9393861.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHBFA_000337"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHBFA_000337"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9393862.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHBFA_000338"
     CDS             complement(8053..8523)
                     /locus_tag="ACHBFA_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM9393863.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHBFA_000339"
     CDS             complement(8569..8994)
                     /locus_tag="ACHBFA_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9393864.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHBFA_000340"
     CDS             complement(9061..9330)
                     /locus_tag="ACHBFA_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9393865.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHBFA_000341"
     CDS             complement(9341..10936)
                     /locus_tag="ACHBFA_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9393866.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHBFA_000342"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHBFA_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9393867.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHBFA_000343"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHBFA_000343"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9393868.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHBFA_000344"
     CDS             15402..16376
                     /locus_tag="ACHBFA_000344"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9393869.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHBFA_000345"
     CDS             16546..17946
                     /locus_tag="ACHBFA_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM9393870.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHBFA_000346"
     CDS             complement(18024..18746)
                     /locus_tag="ACHBFA_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9393871.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHBFA_000347"
     CDS             complement(18825..19331)
                     /locus_tag="ACHBFA_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9393872.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHBFA_000348"
     CDS             19454..21253
                     /locus_tag="ACHBFA_000348"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM9393873.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHBFA_000349"
     CDS             complement(21321..22895)
                     /locus_tag="ACHBFA_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM9393874.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHBFA_000350"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHBFA_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9393875.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHBFA_000351"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHBFA_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9393876.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACHBFA_000352"
     CDS             complement(24672..25310)
                     /locus_tag="ACHBFA_000352"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9393877.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHBFA_000353"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHBFA_000353"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9393878.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHBFA_000354"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHBFA_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9393879.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHBFA_000355"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHBFA_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM9393880.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACHBFA_000356"
     CDS             complement(29104..29844)
                     /locus_tag="ACHBFA_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9393881.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHBFA_000357"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHBFA_000357"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9393882.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHBFA_000358"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHBFA_000358"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9393883.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHBFA_000359"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHBFA_000359"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9393884.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHBFA_000360"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHBFA_000360"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9393885.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHBFA_000361"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHBFA_000361"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9393886.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHBFA_000362"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHBFA_000362"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9393887.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DAYYNG010000009        34957 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000009 DAYYNG010000000
VERSION     DAYYNG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.009"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHBFA_000363"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHBFA_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM9393888.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHBFA_000364"
     CDS             complement(2020..2922)
                     /locus_tag="ACHBFA_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9393889.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHBFA_000365"
     CDS             complement(2919..3611)
                     /locus_tag="ACHBFA_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM9393890.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHBFA_000366"
     CDS             complement(3654..4295)
                     /locus_tag="ACHBFA_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM9393891.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHBFA_000367"
     CDS             complement(4333..4647)
                     /locus_tag="ACHBFA_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393892.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBFA_000368"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBFA_000368"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM9393893.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBFA_000369"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBFA_000369"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9393894.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHBFA_000370"
     CDS             complement(5984..6916)
                     /locus_tag="ACHBFA_000370"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9393895.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHBFA_000371"
     CDS             complement(6913..7689)
                     /locus_tag="ACHBFA_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9393896.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBFA_000372"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBFA_000372"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9393897.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHBFA_000373"
     CDS             complement(10933..12009)
                     /locus_tag="ACHBFA_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9393898.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHBFA_000374"
     CDS             complement(12014..13294)
                     /locus_tag="ACHBFA_000374"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9393899.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHBFA_000375"
     CDS             complement(13313..14239)
                     /locus_tag="ACHBFA_000375"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9393900.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHBFA_000376"
     CDS             complement(14252..15535)
                     /locus_tag="ACHBFA_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9393901.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIDGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBFA_000377"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBFA_000377"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9393902.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHBFA_000378"
     CDS             complement(16323..17234)
                     /locus_tag="ACHBFA_000378"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9393903.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBFA_000379"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBFA_000379"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9393904.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHBFA_000380"
     CDS             complement(17700..18398)
                     /locus_tag="ACHBFA_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9393905.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHBFA_000381"
     CDS             complement(18385..18771)
                     /locus_tag="ACHBFA_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393906.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHBFA_000382"
     CDS             complement(18918..20588)
                     /locus_tag="ACHBFA_000382"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9393907.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHBFA_000383"
     CDS             20771..21004
                     /locus_tag="ACHBFA_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9393908.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHBFA_000384"
     CDS             complement(21103..21534)
                     /locus_tag="ACHBFA_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM9393909.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHBFA_000385"
     CDS             21613..22026
                     /locus_tag="ACHBFA_000385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM9393910.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHBFA_000386"
     CDS             complement(22064..23212)
                     /locus_tag="ACHBFA_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9393911.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHBFA_000387"
     CDS             complement(23334..23930)
                     /locus_tag="ACHBFA_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9393912.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBFA_000388"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBFA_000388"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9393913.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHBFA_000389"
     CDS             27160..27591
                     /locus_tag="ACHBFA_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9393914.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHBFA_000390"
     CDS             complement(27636..28985)
                     /locus_tag="ACHBFA_000390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9393915.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEVATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHBFA_000391"
     CDS             complement(29055..30218)
                     /locus_tag="ACHBFA_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9393916.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHBFA_000392"
     CDS             30347..30670
                     /locus_tag="ACHBFA_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393917.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHBFA_000393"
     CDS             complement(30853..32202)
                     /locus_tag="ACHBFA_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9393918.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHBFA_000394"
     CDS             complement(32271..33224)
                     /locus_tag="ACHBFA_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM9393919.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHBFA_000395"
     CDS             complement(33326..34516)
                     /locus_tag="ACHBFA_000395"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM9393920.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34957)
                     /locus_tag="ACHBFA_000396"
     CDS             complement(34716..>34957)
                     /locus_tag="ACHBFA_000396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393921.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9949 a         7640 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaacg atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccat
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga agaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagcta cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttcc
//
LOCUS       DAYYNG010000010        34928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000010 DAYYNG010000000
VERSION     DAYYNG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.010"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACHBFA_000397"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACHBFA_000397"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9393922.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACHBFA_000398"
     CDS             1011..2258
                     /locus_tag="ACHBFA_000398"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9393923.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACHBFA_000399"
     CDS             2334..2678
                     /locus_tag="ACHBFA_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393924.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACHBFA_000400"
     CDS             complement(2795..3121)
                     /locus_tag="ACHBFA_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393925.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4695)
                     /locus_tag="ACHBFA_000401"
                     /pseudo
     CDS             complement(3494..4695)
                     /locus_tag="ACHBFA_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4812..5327
                     /locus_tag="ACHBFA_000402"
     CDS             4812..5327
                     /locus_tag="ACHBFA_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9393926.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="ACHBFA_000403"
     CDS             complement(5526..5972)
                     /locus_tag="ACHBFA_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9393927.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="ACHBFA_000404"
     CDS             complement(6019..7497)
                     /gene="msr(C)"
                     /locus_tag="ACHBFA_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9393928.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="ACHBFA_000405"
     CDS             complement(7851..8480)
                     /locus_tag="ACHBFA_000405"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9393929.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="ACHBFA_000406"
     CDS             complement(8464..8928)
                     /locus_tag="ACHBFA_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9393930.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="ACHBFA_000407"
     CDS             complement(9415..10683)
                     /locus_tag="ACHBFA_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9393931.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="ACHBFA_000408"
     CDS             complement(10914..11651)
                     /locus_tag="ACHBFA_000408"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9393932.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="ACHBFA_000409"
     CDS             complement(11718..12287)
                     /locus_tag="ACHBFA_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9393933.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="ACHBFA_000410"
     CDS             12415..13287
                     /locus_tag="ACHBFA_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9393934.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13629..14501
                     /locus_tag="ACHBFA_000411"
     CDS             13629..14501
                     /locus_tag="ACHBFA_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9393935.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14498..16456
                     /gene="nagE"
                     /locus_tag="ACHBFA_000412"
     CDS             14498..16456
                     /gene="nagE"
                     /locus_tag="ACHBFA_000412"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9393936.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16707..17558)
                     /locus_tag="ACHBFA_000413"
     CDS             complement(16707..17558)
                     /locus_tag="ACHBFA_000413"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9393937.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17681..18148
                     /locus_tag="ACHBFA_000414"
     CDS             17681..18148
                     /locus_tag="ACHBFA_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9393938.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18411..18893)
                     /locus_tag="ACHBFA_000415"
     CDS             complement(18411..18893)
                     /locus_tag="ACHBFA_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9393939.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18993..19856)
                     /locus_tag="ACHBFA_000416"
     CDS             complement(18993..19856)
                     /locus_tag="ACHBFA_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9393940.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20454..21302
                     /locus_tag="ACHBFA_000417"
     CDS             20454..21302
                     /locus_tag="ACHBFA_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM9393941.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21473..21814)
                     /locus_tag="ACHBFA_000418"
     CDS             complement(21473..21814)
                     /locus_tag="ACHBFA_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFM9393942.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21900..22607
                     /locus_tag="ACHBFA_000419"
     CDS             21900..22607
                     /locus_tag="ACHBFA_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9393943.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22594..22917
                     /locus_tag="ACHBFA_000420"
     CDS             22594..22917
                     /locus_tag="ACHBFA_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9393944.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23006..24043)
                     /locus_tag="ACHBFA_000421"
     CDS             complement(23006..24043)
                     /locus_tag="ACHBFA_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9393945.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24490..25473)
                     /locus_tag="ACHBFA_000422"
     CDS             complement(24490..25473)
                     /locus_tag="ACHBFA_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM9393946.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25889..26659)
                     /locus_tag="ACHBFA_000423"
     CDS             complement(25889..26659)
                     /locus_tag="ACHBFA_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9393947.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26652..27587)
                     /locus_tag="ACHBFA_000424"
     CDS             complement(26652..27587)
                     /locus_tag="ACHBFA_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393948.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28181..29830)
                     /locus_tag="ACHBFA_000425"
     CDS             complement(28181..29830)
                     /locus_tag="ACHBFA_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9393949.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30025..30921)
                     /locus_tag="ACHBFA_000426"
     CDS             complement(30025..30921)
                     /locus_tag="ACHBFA_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9393950.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30914..31594)
                     /locus_tag="ACHBFA_000427"
     CDS             complement(30914..31594)
                     /locus_tag="ACHBFA_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9393951.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31595..32377)
                     /locus_tag="ACHBFA_000428"
     CDS             complement(31595..32377)
                     /locus_tag="ACHBFA_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9393952.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32370..33287)
                     /locus_tag="ACHBFA_000429"
     CDS             complement(32370..33287)
                     /locus_tag="ACHBFA_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9393953.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33290..33574)
                     /locus_tag="ACHBFA_000430"
     CDS             complement(33290..33574)
                     /locus_tag="ACHBFA_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393954.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34275..>34928)
                     /locus_tag="ACHBFA_000431"
     CDS             complement(34275..>34928)
                     /locus_tag="ACHBFA_000431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393955.1"
                     /translation="SIYSVICSSQQVNINRVIKLIGAKNINKVRSEFIFPEDFFNWFF
                     YQFSECKRLLGENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKN
                     VTARIRKENEVDIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLR
                     SLYMSSESIFVSEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
BASE COUNT        10464 a         7287 c         6196 g        10981 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    13441 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    13501 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    13561 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    13621 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    13681 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    13741 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    13801 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    13861 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    13921 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    13981 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    14041 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    14101 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    14161 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    14221 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    14281 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    14341 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    14401 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    14461 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    14521 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    14581 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    14641 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    14701 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    14761 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    14821 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    14881 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    14941 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    15001 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    15061 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    15121 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    15181 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    15241 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    15301 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    15361 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    15421 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    15481 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    15541 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    15601 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    15661 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    15721 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    15781 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    15841 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    15901 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    15961 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    16021 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    16081 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    16141 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    16201 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    16261 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    16321 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    16381 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    16441 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    16501 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    16561 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    16621 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    16681 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    16741 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    16801 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    16861 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    16921 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    16981 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    17041 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    17101 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    17161 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    17221 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    17281 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    17341 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    17401 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    17461 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    17521 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    17581 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    17641 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    17701 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    17761 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    17821 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    17881 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    17941 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    18001 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    18061 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    18121 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    18181 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    18241 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    18301 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    18361 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    18421 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    18481 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    18541 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    18601 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    18661 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    18721 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    18781 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    18841 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    18901 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    18961 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    19021 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    19081 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    19141 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    19201 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    19261 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    19321 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    19381 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    19441 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    19501 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt
    19561 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    19621 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    19681 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    19741 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    19801 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    19861 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    19921 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    19981 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    20041 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    20101 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    20161 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    20221 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
    20281 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
    20341 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
    20401 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
    20461 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
    20521 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
    20581 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
    20641 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
    20701 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
    20761 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg
    20821 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
    20881 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
    20941 tcatggagat gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct
    21001 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
    21061 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
    21121 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
    21181 tcgatcatac tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact
    21241 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt
    21301 aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac
    21361 aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag
    21421 acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt
    21481 catgacaaat ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat
    21541 acttttctac attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac
    21601 aacaactttc aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta
    21661 atgcaggtcc ttcctccata cttaaacaat agaccgttgc atcgaaaata tcagcttgga
    21721 tttgtagcca tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc
    21781 gacccgctag ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga
    21841 atattcacaa gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
    21901 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt
    21961 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag
    22021 tcgtcacgtt gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca
    22081 tgcttgctag tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc
    22141 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
    22201 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
    22261 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
    22321 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
    22381 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
    22441 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
    22501 ttctggtata tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac
    22561 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
    22621 actattttag gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg
    22681 aaaaaattcg atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
    22741 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
    22801 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
    22861 ttattggtga tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata
    22921 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
    22981 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
    23041 ctaacatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
    23101 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
    23161 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
    23221 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
    23281 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa
    23341 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
    23401 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
    23461 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
    23521 gttgattcaa aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc
    23581 gattggcatt ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca
    23641 atgaggtcaa cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga
    23701 caatacgttc gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg
    23761 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt
    23821 tcccgactcc atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa
    23881 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
    23941 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc
    24001 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
    24061 ttcatcaagt tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt
    24121 ccaccttttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    24181 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct
    24241 accaactctt cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat
    24301 tgctccttgt gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca
    24361 atcaactgac cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt
    24421 ggctgttctt attacttgat acaggacagc tttttcacga cctctaattc acggtgttca
    24481 agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat
    24541 ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc
    24601 tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat
    24661 gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt
    24721 ttctgtatgt tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc
    24781 aatcatgatg ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg
    24841 gatatctcca ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat
    24901 tttaggaatg acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg
    24961 gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga
    25021 catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc
    25081 ttcaatcaat tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc
    25141 tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc
    25201 catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat
    25261 cggctgttca tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg
    25321 acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt
    25381 tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg
    25441 ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag
    25501 cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag
    25561 caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc
    25621 ttatcacagt ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag
    25681 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    25741 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25801 cgctcaaaaa acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    25861 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    25921 aataccaagt aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg
    25981 ctactggcac atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    26041 tcggattgac ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa
    26101 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat
    26161 aagactgagc aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca
    26221 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    26281 tgatcgttac gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc
    26341 aaataatcac atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt
    26401 tttgagagaa gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa
    26461 tcaaaccagg aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac
    26521 cgatcatttt tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg
    26581 tttggaccca tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    26641 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag
    26701 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    26761 gtaagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    26821 tgctcatctt caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct
    26881 aaatcaaaga taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc
    26941 tcgatcagtt cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct
    27001 agataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc
    27061 cacatctcgc gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa
    27121 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    27181 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    27241 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    27301 tggaccgttt caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt
    27361 gctcgaacta gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta
    27421 ttgactaaag aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat
    27481 agcgcataga aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca
    27541 cttccataga tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    27601 ccttatagac atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc
    27661 ttccacacaa aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc
    27721 ttcgatagaa taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag
    27781 caactactac gatgacatat agaagatcct tataactgag gttcttgtct tttttattca
    27841 tgttaagctt tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa
    27901 ataaaatgga aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt
    27961 gccatggaac gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg
    28021 aagacagcgt ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag
    28081 cagactcgtt cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat
    28141 cgtctttttg gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact
    28201 gacgctgttc atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat
    28261 aattcaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    28321 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    28381 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    28441 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    28501 ttccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    28561 gcaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    28621 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    28681 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    28741 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta
    28801 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    28861 ctggtcggca agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca
    28921 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    28981 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    29041 attctgcttc actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta
    29101 atggtttagt ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat
    29161 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    29221 gtttcttcac tagataatag accgtattga atactaagcc accgattgct agatagattc
    29281 ctagtacttg ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa
    29341 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    29401 aattatgatc tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag
    29461 gattttcctg ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc
    29521 tacgtaaacg ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt
    29581 caaatacttc gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg
    29641 aacggaattt tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg
    29701 gttgataagg aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa
    29761 aaatgtctaa aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc
    29821 tttggcccat atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct
    29881 atttgaaaag ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca
    29941 aaataaaaac accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat
    30001 tcacttcagg taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat
    30061 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    30121 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    30181 ctggaagact ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa
    30241 tcgaatgaat gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    30301 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    30361 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    30421 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    30481 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    30541 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    30601 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    30661 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    30721 attttcatcc acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    30781 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    30841 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    30901 aagcaacagc cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    30961 catgtgcctt tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac
    31021 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    31081 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    31141 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    31201 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    31261 acaacagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    31321 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    31381 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    31441 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    31501 caagaagttt gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt
    31561 cttcgataat caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa
    31621 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    31681 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    31741 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg
    31801 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    31861 tcaaagccac tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt
    31921 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    31981 tgtaagcaat catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg
    32041 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    32101 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    32161 gaagaatcca tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg
    32221 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    32281 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    32341 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    32401 aagaaataat cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc
    32461 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    32521 cggctagatt ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    32581 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    32641 gataattctt tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac
    32701 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    32761 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    32821 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    32881 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    32941 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    33001 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    33061 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    33121 cccataatag ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag
    33181 atcattccac gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt
    33241 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    33301 tcttattgat aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    33361 gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt
    33421 gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
    33481 tggtaatcgt tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct
    33541 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
    33601 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
    33661 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
    33721 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
    33781 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
    33841 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
    33901 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
    33961 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
    34021 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
    34081 gttaaacaaa actctatttt attgcatttt acaggaatgt atttcatgcg aaattaataa
    34141 gtctttgttt atagagtttt actagggtat aaaaagttgt atgttatcat tacctcaaaa
    34201 cattacctca gcttggcgag ttggttgatt gaagaataaa tcatttatct agatatctca
    34261 ataaaaaatg atttctaaga aacttgtatt ttcgtgttat tatttttttg ccctatcaaa
    34321 atttctaatt cttctagcgt aatatgatta tgacacataa tcgattcaat tacttcaata
    34381 cctatttttt tcgagaaaga tcccttttgt tcactcacaa aaatactttc agaagacata
    34441 tataaggaac gaagttttgg aattagtata tcgtacaaat agaccaccat aaaatcgtcc
    34501 ttgcaaccat tctcaaaaag aattaaattt gctgattgtg ttacatatat cactgaatta
    34561 cattcatggt caattccaaa aacaatgtct acttcatttt cttttctaat tcgcgcagtt
    34621 acatttttca aactttcacc attacttata aatgctttag ttactgtaag ctctgatgtt
    34681 tgttcagata ttccttttat aatattttgt tcatcagcca cattccccat aaatccgccg
    34741 attcctttta agtaataatt ctcacctaag agtcgcttgc attcactgaa ttgataaaaa
    34801 aaccagttaa aaaaatcttc tggaaaaata aattctgatc gaactttatt tatattttta
    34861 gctcctatta attttataac tcgattaatg ttcacttgct gcgatgaaca tataactgaa
    34921 taaattga
//
LOCUS       DAYYNG010000011        34685 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000011 DAYYNG010000000
VERSION     DAYYNG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.011"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(338..2230)
                     /locus_tag="ACHBFA_000432"
     CDS             complement(338..2230)
                     /locus_tag="ACHBFA_000432"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393956.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2227..3954)
                     /locus_tag="ACHBFA_000433"
     CDS             complement(2227..3954)
                     /locus_tag="ACHBFA_000433"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9393957.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3970..4620)
                     /locus_tag="ACHBFA_000434"
     CDS             complement(3970..4620)
                     /locus_tag="ACHBFA_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM9393958.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4824..5120
                     /locus_tag="ACHBFA_000435"
     CDS             4824..5120
                     /locus_tag="ACHBFA_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9393959.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5312..5932
                     /locus_tag="ACHBFA_000436"
     CDS             5312..5932
                     /locus_tag="ACHBFA_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9393960.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6024..7190)
                     /locus_tag="ACHBFA_000437"
     CDS             complement(6024..7190)
                     /locus_tag="ACHBFA_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9393961.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7202..7555)
                     /locus_tag="ACHBFA_000438"
     CDS             complement(7202..7555)
                     /locus_tag="ACHBFA_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9393962.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7683..8705
                     /locus_tag="ACHBFA_000439"
     CDS             7683..8705
                     /locus_tag="ACHBFA_000439"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9393963.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8724..8885
                     /locus_tag="ACHBFA_000440"
     CDS             8724..8885
                     /locus_tag="ACHBFA_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393964.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9144..9578)
                     /locus_tag="ACHBFA_000441"
     CDS             complement(9144..9578)
                     /locus_tag="ACHBFA_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393965.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9688..11547)
                     /locus_tag="ACHBFA_000442"
     CDS             complement(9688..11547)
                     /locus_tag="ACHBFA_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9393966.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11548..11688
                     /locus_tag="ACHBFA_000443"
     CDS             11548..11688
                     /locus_tag="ACHBFA_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM9393967.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKCSLSLWPFW
                     FL"
     gene            complement(11665..12824)
                     /locus_tag="ACHBFA_000444"
                     /pseudo
     CDS             complement(11665..12824)
                     /locus_tag="ACHBFA_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13131..14555)
                     /gene="araA"
                     /locus_tag="ACHBFA_000445"
     CDS             complement(13131..14555)
                     /gene="araA"
                     /locus_tag="ACHBFA_000445"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM9393968.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14571..15269)
                     /locus_tag="ACHBFA_000446"
     CDS             complement(14571..15269)
                     /locus_tag="ACHBFA_000446"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9393969.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15226..16887)
                     /locus_tag="ACHBFA_000447"
     CDS             complement(15226..16887)
                     /locus_tag="ACHBFA_000447"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9393970.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17105..18505
                     /locus_tag="ACHBFA_000448"
     CDS             17105..18505
                     /locus_tag="ACHBFA_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM9393971.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLIQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18757..19836)
                     /locus_tag="ACHBFA_000449"
     CDS             complement(18757..19836)
                     /locus_tag="ACHBFA_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9393972.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19922..20881)
                     /locus_tag="ACHBFA_000450"
     CDS             complement(19922..20881)
                     /locus_tag="ACHBFA_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9393973.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20893..22404)
                     /locus_tag="ACHBFA_000451"
     CDS             complement(20893..22404)
                     /locus_tag="ACHBFA_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9393974.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22426..22989)
                     /locus_tag="ACHBFA_000452"
     CDS             complement(22426..22989)
                     /locus_tag="ACHBFA_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9393975.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22995..23858)
                     /locus_tag="ACHBFA_000453"
     CDS             complement(22995..23858)
                     /locus_tag="ACHBFA_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9393976.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23872..24768)
                     /locus_tag="ACHBFA_000454"
     CDS             complement(23872..24768)
                     /locus_tag="ACHBFA_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9393977.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24943..26232)
                     /locus_tag="ACHBFA_000455"
     CDS             complement(24943..26232)
                     /locus_tag="ACHBFA_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9393978.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26204..27721)
                     /locus_tag="ACHBFA_000456"
     CDS             complement(26204..27721)
                     /locus_tag="ACHBFA_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM9393979.1"
                     /translation="MEATMFISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27919..28809
                     /locus_tag="ACHBFA_000457"
     CDS             27919..28809
                     /locus_tag="ACHBFA_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM9393980.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28882..29706)
                     /gene="nadE"
                     /locus_tag="ACHBFA_000458"
     CDS             complement(28882..29706)
                     /gene="nadE"
                     /locus_tag="ACHBFA_000458"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9393981.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29718..31190)
                     /locus_tag="ACHBFA_000459"
     CDS             complement(29718..31190)
                     /locus_tag="ACHBFA_000459"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9393982.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31827..32189)
                     /locus_tag="ACHBFA_000460"
     CDS             complement(31827..32189)
                     /locus_tag="ACHBFA_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9393983.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32412..33164
                     /locus_tag="ACHBFA_000461"
     CDS             32412..33164
                     /locus_tag="ACHBFA_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9393984.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33304..34227)
                     /locus_tag="ACHBFA_000462"
     CDS             complement(33304..34227)
                     /locus_tag="ACHBFA_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9393985.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34242..34514)
                     /locus_tag="ACHBFA_000463"
     CDS             complement(34242..34514)
                     /locus_tag="ACHBFA_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9393986.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10237 a         7216 c         5984 g        11248 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc
       61 acaaggagca atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg
      121 aggagttggc aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga
      181 gtattagaga tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata
      241 ccgaaggaca gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg
      301 tcacagcccc taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa
      361 actgactgtt ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc
      421 ctgtttctac gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag
      481 acaagcggtg agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt
      541 gaatcagtat ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat
      601 ctggatttgc taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat
      661 tgcttgcatc ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt
      721 ctacgtgagc agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat
      781 atttgatatt gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa
      841 actgtgcgcg caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac
      901 tacctccaga aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac
      961 cagtcggacc tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca
     1021 ttagcaagtt atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg
     1081 tatctttttc ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt
     1141 caaatacccg ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt
     1201 gagtgattgg ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg
     1261 tcatgttccc atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat
     1321 ttttaacgaa attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag
     1381 cggcatacag attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga
     1441 atgttttaac aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta
     1501 aacttttttg ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga
     1561 tcaagcttaa agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca
     1621 tcatcacgat gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct
     1681 gcagcgtgct tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg
     1741 tatgtgtatc ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta
     1801 attcataaac tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata
     1861 cagcagaaat caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt
     1921 ctatcggaaa ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac
     1981 ctttgaatat ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca
     2041 cggctgctat tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc
     2101 caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat
     2161 ttctgccagg tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg
     2221 ttttactcat gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc
     2281 gtttgttgct aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac
     2341 aaggatcata tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact
     2401 ttctttcata ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc
     2461 tgagaaggaa tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc
     2521 cagacgctgt ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa
     2581 gccatcttcc atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat
     2641 catttcatct gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa
     2701 cagaactgct ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc
     2761 actaatgtcc gttccattta tttggatcga accagattcg atgtcatata atcgagggat
     2821 caaattgact aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc
     2881 cgacttggct tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata
     2941 attgacatga ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt
     3001 ttcagggtca tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc
     3061 ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg
     3121 catcgcataa gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta
     3181 gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg
     3241 catcatcaat gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc
     3301 taaatcaaaa cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc
     3361 tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa
     3421 taatggaaca gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac
     3481 taagaagatt tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa
     3541 cgtgattggt gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt
     3601 cacatcattg gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt
     3661 tgtcagactc tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga
     3721 ttcacgagta gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc
     3781 gatcatcaca aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc
     3841 aataatattg gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa
     3901 aactgcagct gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt
     3961 cccttctttc tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact
     4021 gattgcttgc tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat
     4081 tgttgtaaat acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt
     4141 tactttcagc ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc
     4201 tttatgtgga tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc
     4261 acggtaatcc atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat
     4321 ctcataaatc attttagaaa aataggattc gaatcctttg aatagatcac catcggattc
     4381 tttcattagt tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa
     4441 ataaagatcc tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa
     4501 tttgacgata ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc
     4561 agcctcgatt agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat
     4621 atggattctc ctttctttca agatgacaca ttgtcatatg acgctatgtc attataacac
     4681 ctcctacctg ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa
     4741 aaagtgataa gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac
     4801 atactaaaca aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat
     4861 tgcctctcga tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa
     4921 accacttaag ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc
     4981 tagatccaaa gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag
     5041 ccgatcaact tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc
     5101 ttcagtcgag tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat
     5161 cgtgtcacag cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca
     5221 atatgacttg atagcctttt ctttttcgag taaactgatt ttgttagtca tagttataac
     5281 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5341 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5401 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5461 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5521 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5581 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5641 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5701 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5761 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5821 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5881 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5941 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg tgccttggat
     6001 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     6061 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     6121 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6181 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6241 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6301 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6361 gggatcaggt ttaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6421 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6481 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6541 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6601 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6661 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6721 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6781 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6841 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6901 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6961 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     7021 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     7081 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     7141 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7201 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7261 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7321 ctactgtctt taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7381 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7441 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7501 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7561 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7621 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7681 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7741 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7801 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7861 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7921 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7981 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     8041 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     8101 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     8161 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8221 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8281 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8341 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8401 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8461 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8521 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8581 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8641 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8701 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8761 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8821 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8881 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8941 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     9001 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     9061 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     9121 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9181 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9241 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9301 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9361 gtactgactt tcataagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9421 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9481 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9541 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9601 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9661 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9721 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9781 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9841 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9901 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9961 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
    10021 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
    10081 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
    10141 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10201 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10261 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10321 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10381 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10441 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10501 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10561 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10621 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10681 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10741 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10801 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10861 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10921 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10981 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    11041 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    11101 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    11161 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11221 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11281 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11341 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11401 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11461 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11521 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11581 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11641 atgaaatcca aatgttctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11701 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11761 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11821 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11881 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11941 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    12001 tccgctgtct gccaaagctt cctgagtcga tcccctagat gaggcaacat aagtatacgg
    12061 tcattttcta agaactcttc ttctggaaaa tcagtcaagt atttagccag atgttgtttt
    12121 tccaatgctt gcataacagc atatttatca acaatttcac ttcttcctac attgataagt
    12181 acagcatctt ttttcatttt ttctatattc gcctgatcga tcaatccttt tgtatcttct
    12241 gtcaacggca gcatgactac gatgtaatcc gaggtagaaa gaactgtttc taaatctgct
    12301 tgccgaacat aatccaactg ggcatcgcgg atagaataac ccaagacatc catccctaag
    12361 ctatagcaac ttagcgctac ttttgtgcct atcgctccaa gaccaagaag accgatggtt
    12421 ttgccttgca attctcgtcc cacataagca cttcttttgt tttctgcctg ttctaaaata
    12481 ttaggacctg ttagtgtttg taccatacga cttgcttcga ttatcggacg ggaagataag
    12541 agcaaacagc acaaaacaag ctcttttact gcattagcat ttacacctgg tgtattcatg
    12601 acgatcgtcc cgttttcaga agctttttca acgttgatcg tgttcacccc aacaccagct
    12661 cttgaaattg ctagcaaatc tggtgtaagc cattcatctt tcactttcgt actacgaata
    12721 actattcctt cggctggcaa atcatctatt attttgaaat attccccttg taacttttct
    12781 atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat acataaaacc ccctccttac
    12841 taacaagtat ctcgaaattt ctgcaattag acaactgttt accatttgac ttcctagaag
    12901 caaaaagagc agttagagca gagtaggctg agtgataaaa ataaggctat gacaagttac
    12961 tgccataagc cttattttcg agagcaaaat attgagggtg agatatcttg ataaatggtg
    13021 ttccgttagc tgacattgat aataagacta aatttcaaaa aagttgaaaa gcaaatttcg
    13081 tcaatctctt cttatttctt aaaactaagt ggctttttca caacctcttt ttatctaata
    13141 accactgttt ccaactcaac taattttgcc cacgtttcaa tttgatctgt tgttagattt
    13201 agcgagacaa cggtatgatg accgccacct gcctgaatcc atgaatggac accttcatgg
    13261 aaattcggtt tagttttcca taacacacgt gcaacaggta aattaggtgc ttcagtcgtt
    13321 ggttcaaagg cttctacttc attgatcagc agtttataat gcgtaccgaa atctgccatc
    13381 gatacgacta ccccttctcc tgctttccca tcaaatacaa gacgcgccgg atcttctcta
    13441 tctcccattg ataaaggaga aacaacgatt cttggtttcg tcgcagcaag cgtcggatct
    13501 acttccatca tatgggattc caagattgct tcttggccac tagctagctc gtaagtataa
    13561 tcttccatga atcctgtatc tttccctcga gccatgattt tcagcaaccg atcaatcgct
    13621 gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca
    13681 gctagtccag gtagttgttt cattccatac aggtcttcaa aattagtcgt aaaagcgtta
    13741 tagcctcctt cttctaagaa acggcgaata ccgatttcct gctgagcttg tacttttaca
    13801 tgttcctccc atgtcttttc ttcataatca ccatagtcga aggtatacaa ttccttgtac
    13861 tcttcaaata attgatcgac ttcttcgtct gatacgcgat cgattacttg tacaagatct
    13921 ccgataccaa aataatcaac agtccaacca aattgaatct gtgcttccac tttgtctcct
    13981 tctgtgacac ccacgtttct catgttgtca ccaaaacgag ccacttttat tccaaaactt
    14041 tcattatagg ctactgcaac atccatccaa tcagcaatct ctttttggat ctcaggtcgt
    14101 ttccagtagc caacgacgat tttattttgt ttatttaatc gagcattgat gaacccatat
    14161 tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa
    14221 ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc
    14281 ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc aagtgatcac acctgctact
    14341 tcttctcgat aattgacctc tttcatgatt tttgtgatcg tatcagcagt aactgctaat
    14401 tcttgaaggc ggattggata aggcaattgg ccattttcat tcagttcatc gaccatttct
    14461 tgtgcatgtt tctttacttc tcgcagtgct tcttccccat ataaatgctg tgatcctaca
    14521 acaaaccaaa attctttttc tccaatattc aacataatta gccactcctt ttatttgttt
    14581 tgtccgtaat acgcattttt tccgtgtttt cgtaaataat gtttatctaa aatccgttga
    14641 ggcagctctt ctgaaaatga attgagctgt ctggtaaata aattcatctt gcatacttca
    14701 tctagtacga ctgcgttcat taccgcactt tgcgcatctt tcccccaagt aaatggaccg
    14761 tgtccgtgta aaagtacccc tggaattgct aagggatcaa ttttgcgttc tttgaatgtt
    14821 tctatgataa cttttcctgt ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa
    14881 aatcgagcac aaggtaccga tccataaaaa gtatctgcat gcgtcgtccc catcgcagga
    14941 acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg agtgagtatg gacgattcct
    15001 ccgatttgag gaaatgaacg atataaaaca gcatgagtag gcgtatccga agagggattg
    15061 agtttacctt caatgatatg attgtccaag tccacaacga ccatgtcgtc tgctgtcatc
    15121 tcttcataac tgactccgct aggtttgata acgaaatatc gtgtttcagg atcaaaagcg
    15181 ctcacatttc cccatgtata tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg
    15241 tatacttctt ctttcagttg ttccagcatt ttctatcact cctttttctt gcaattgatg
    15301 aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt ctcataaata tctgatagcc
    15361 tgctatttct tctgctgaag gtgtgatggt gattccttgg tcttgtccaa acacgtcttc
    15421 tgccaagaag ttttctaaag acttcccatg tcgatccatc atataggctg ctaataacgc
    15481 gattccccac gctccacctt ctccggctgt gtccatcact gtaactggtg cctccatagc
    15541 agatgctaga atccgctgtc caacttctgg tgttttaaaa atccccccat gccccactaa
    15601 acgatcaatt tggacattct cagatttcaa tatatccatt ccgattctca ttgcaccaaa
    15661 agcactggct aaatggatcc gcatgaaatt cgctaaatca aaacggctac ctggtgtacg
    15721 tacaaataat ggccttcctt catttactcc tgtaatgttt tcaccggaaa aataaccata
    15781 agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa
    15841 taatttatct ctgcttatct ccatacccaa agaattggta aattcttcaa aaatactgac
    15901 ccaagcattg atatccgaag agcagttatt cgtatgaacc atgcctacta gttctcctga
    15961 aggtgtagtg accatgtcga tttccggatg gacattttct agttcttttt ctaatacgat
    16021 catagcgaag gcggaagtac cagccgaaat gttccctgtc cctttggcta cactgttcgt
    16081 agcgaccata ccggttcctg catccccttc tggcggacaa accgggattc ctggtctcaa
    16141 atttcccgta gggtcgatca gataagcccc tgattcgctt aacttgccag cttcttctcc
    16201 tgctactaga actttaggca aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat
    16261 taactgacca aaaatatttt tcatttcttg atggtaggtt ttcgttgaag gatcgatagg
    16321 aaacatgcct gatgcatctc ctacgcctag tactttctca ccagtaagct gccaatggac
    16381 ataacctgca agcgttgtca aaaaattaat ttctttcaca tgttcttctt tattcaaaat
    16441 agattgatac aaatgagcga tactccatct ctgcggaata ttgtattgaa atttttccgt
    16501 taatattcgt tctgcttctt cagtgattgt atttcgccaa gtacgaaatg gtactagtaa
    16561 ctgttccttg ctatcaaatg ctagataacc gtgcatcatt gcactgaacc caatcgaccc
    16621 gattgtcgta agattctcgc catagatttc gaatacttct gctgccattt ttcggtaact
    16681 gacgcgcagt cctttccaaa tatcttctaa tgaataggtc cagataccat tttctagttt
    16741 gttttcccac tcataactac cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc
    16801 tttgatccta gttgatccaa actcaatccc taaagatgta cgaccatcta tgacatctaa
    16861 catactttgc gcatttactt ccttcataat gatttaactc cctccttctc gtcaaacctg
    16921 atcaaaccgt ctttttagta gcgcttacaa tgattattgt atacttttgt acgtacatgt
    16981 caagcagtta tttacaagaa tacaataaaa aacacttgtt ttgtacgtac ataagttata
    17041 aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg aaattctaga aggagagaat
    17101 acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt tatttttttg gcgcatttgg
    17161 cggcattcta tttggatatg atattggtgt tatgaccggc gccttacctt ttttacagca
    17221 tgattggggt ttggcaggta aagcttcttt gataggatgg atcacgtctt cggtcatgtt
    17281 aggtgcgatc cttggcggct ctttatctgg tcttctttct gataagcttg gacgacgaaa
    17341 aatgattctt ctttctgcac ttatttttat ggctggttct gtcctttctg catctgcccc
    17401 tcacaatggt tcttattttc tgattgcagc aaggattctt ctaggtttag ctgttggcgc
    17461 tgcatctgcc ctagttcctg cttatatgtc tgaaatggct cctgctagac taaggggaag
    17521 attgtcaggg atcaatcaag tgatgattgc ttctggcatg ctcctttcct atgttgcaga
    17581 ttatttgcta aaagggttgc ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc
    17641 tgtccccgcg ctgattttat ttttcggtgt tttagctcta ccagagtcac cgcgttttct
    17701 tatacaatct ggccgcttgg aagaggcaaa aagagtattg aactatattc ggacaccaaa
    17761 agaagctgaa caggaattcg aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg
    17821 aacttcttgg catactttgt ttttagaaaa atatcgttcc ttagtcattg ccggtattgg
    17881 tgtagcagta ttccagcaat ttcaaggagc taatgctatt ttttactata ttccgctgat
    17941 cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg atgtggccga tcatccaagg
    18001 aatcatttta gttgctggtt ctttgctctt tttggttatt gcagacaagt tcaatcggcg
    18061 aactttattg aaaataggcg gtagcgtgat gggactttct ttcatcctac cagctgtttt
    18121 aggaactgta ctggatgctc atacaaattc gttgttgatt ctcttgtttt tatgtattta
    18181 tgtcgcattt tattcttgca catgggctcc actgacatgg gtaatcgtag gcgaaatatt
    18241 cccactagct gtccgcgggc gagcatctgg cctagcctct tcttttaatt ggattggttc
    18301 tttcctagtt ggcttgcttt ttcctgtgat gactgcttct atgtctcaag aaatcgtttt
    18361 cggcattttt gggatcatct gctttttagg ggttttattt attcaagaga tcgtaccaga
    18421 aactcgtggg aaatctttag aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa
    18481 acgtatttct atccatcatt cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat
    18541 aagaaggaac cgcttagttt ccagtcacaa ggaacaattt caaaaaacag cttctcatgt
    18601 ttgatgaata ttgtgacttg tgaccagttg gtaggagaac accgtttagt ggagaggtgg
    18661 tgaaagaaac gttcagcctt gagcgatgac aaaagctctc acaaacattg cgacgagtaa
    18721 ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt
    18781 ttcccgttca actagtacag gttcatagag aatatttggc tgtgcctgtc ctttttcaat
    18841 cgttgtgacg atccaccgtg cggcatcttg tcccaacttt tcttttggat gcgtcaatgt
    18901 tgtcaatttt ttattcccta aatggcttaa agaagaatcg tctgttccta cgatcgatag
    18961 gtcatctgga atatgatagc cattttctgt caacttatct agcattaaac tagctatttg
    19021 atcgttatag caaatgatgc catctaaagc ttgttcttct ttcaaccgtt gtactgtttt
    19081 ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg
    19141 cacattatac tgttcacacg cgctgataaa acctttcatc ctgtattttc cttgaagatc
    19201 gtcgattttt gtgatgaaca acagacgctg atgtcctttt tttatcaaat attctgctgc
    19261 cataaaaccg actttcacat catctaaaca aatataagag gcatttaact cttcataagc
    19321 agcattgatc atcaccatcg gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt
    19381 tgggttatac tgattacttt tcgtaggctc aacgatcaaa ccatctaccc cataagcaat
    19441 cattttcttc aaacactctt tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa
    19501 tgaataccca gattctcgta attcattttc gattcctcgg ataatcgacg gaaaaatata
    19561 atcagagaga tatgtggtga tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc
    19621 tttcttatag ctgtcatcta catatgtgcc cgaccctttt tctttacgca gatagccctc
    19681 atttaccaac acagcgattg cttgccgaat cgtatgtctg ctgacgtcat atatttcttg
    19741 cagcttgaat tctgagggaa tggttgtatt tacttcgtat tcctcacgaa ggatcttttg
    19801 ttttatctct tctgcaatat cttgatattt tgccatttgc cttgcccctt tttatttgta
    19861 cgtacatata tcataacgag tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg
    19921 ctcataaggc atacggaaca ggttcaccaa attctggaaa tccttgctca ttccacgtaa
    19981 agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg atcccctttt tggttcttct
    20041 ctggtcgagc atgatagatc aaaatatcaa agcacccgtc ttctgaaacc gtaaacgagt
    20101 tgtgaccagg accaaattgc ttattttttt cagaagaaac gaatactggc tccgataatt
    20161 tatgccatga ttcttttaat aaaagattct tgtgctcatc agcccacagc aggcccatgg
    20221 catagttttc atctgttgca ctaccagaat acgtaatgaa tattttccca ttgcgagtaa
    20281 ggactgctgg accttcattt actgaaaaac cccgtttttc ccattcatac tcaggtatag
    20341 aaaggaggat ctgcttcccc tttaacgtcc aaggattatc catttcggat agataaagat
    20401 tagaattccc agtaatttca ggatcttgtt gggcccacac ataatatagc tttccttgag
    20461 aagagaaagt tgttccgtct aaactgaaag tttccctttt cgtttttatt tgtccctttt
    20521 ctatccaatc tgtattcatt ggatctatat gctgattttc aagtacgaac atgcggtgat
    20581 gatgagattc atcacgtata gttttatgat tgcttgcagc aaagtaaata taccattttc
    20641 cattgatata atggacttca ggtgcccaga tcaattcact catcggtcct tcttcatgag
    20701 cctgccatac aatcaggctt gcagcatgtt ctaattcatt cagtgacttt gcccttctta
    20761 attcaatcac ttggtagcct ggaaccgatc ctgtaaaata ataataacca tctgtatgtt
    20821 tatataccca aggatctgcc cgttgccgaa cgataggatt attataaagt gtcttcatca
    20881 taacaaattt cctcacattt tctttattct aattacattc caagagtatt tttctatccc
    20941 tacatttaat tcgtcatgtt tttctattga aaaatagttt atttcaggtt ttattttttc
    21001 tggttcttca cgtgtatttt tgtccaacag atcgctactg gttaatgtgt agtgctctcc
    21061 ttgatctagc aaattgaaat catgttcaat cgttatcttt tgttgttcat ttgtgcgatt
    21121 gatcaagaaa atagtcagct gattgccgtt atctacgatc acactgtcca cataaggaac
    21181 attttttatc ttttcacaat cataaacagg gcaaatgatt tgttgtttca atacttgacc
    21241 tttagcatat ttcgataagt gttgcatcac ataataagtt ggattcctcc atgcttgtcc
    21301 tttagcatct gtcagtatta aaggtattgt gtttactaac aatgattgac aactaatctt
    21361 tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa ccaaataata atgtatcttc
    21421 catagtaaag taacaccaat cgatgggact accgacttca aaatccctat gtcttttttc
    21481 cggttgggca tgtacattcc attcatcaaa cgctagatac attgttttct cacttctcaa
    21541 atatcctttt acataatcac aagtagcaac gatttcttca atttgacgat cgaatcgata
    21601 agcttttgca aggtatgtcg ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc
    21661 ttcttcatca tacttatcaa tatagttatg caaagctaaa taatctacat tctcataagc
    21721 ttcttctaat gcgatacgat cccattcagg gtacgatggt aaacgtggcg tagagcttcc
    21781 aactaagatt gtttcaattg aatcatccac taacttcatg acttttgagg cttcatttgc
    21841 taacctacca tattcataag cactcttagc ccccacttgc catggaccgt ctagctcatt
    21901 ccctaaacac cataatttaa cgccaaaagg ctttttttgc ccatttttta ttcttaaatc
    21961 actccagaag gttccttttg gaaaattaca atattctact aaattttttg cttcatcaat
    22021 tccttttgtt cctaaattca ccgtgaatat cggtttcgct tgtacatgac tgatccattt
    22081 aaaaaattcg tggagtccaa aagaatttgg ttctatacct cgccatgcta aatcaatttt
    22141 ttttggtcgt tgttctttcc tacctacagt atccacccaa tcataaccag atgtaaagtt
    22201 tcctccagga tagcgaatga cacctaattc taattccttt acataagaga ggacatcttg
    22261 tctaaacccg tcaccatcag ctgtttcatg gcccggttca taaatccctg tatagacgac
    22321 acttcccata tgctcaacaa atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa
    22381 atcctctctg atttttactt tcacttcttc gtccaacagc tttccctacc tttcatttcc
    22441 tatagtcaag ataatagata caagtttcca tacgccacca gcaaagataa aaataaaagg
    22501 agggaaaaga aatatgagca gtacaaatcc taataacaat acgcaacaaa gaatatttct
    22561 caaaaaatca aaaaatactt tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc
    22621 tttgttttca gaaatcacat atatccagga taaaagaaac atggaataaa taagtaaaac
    22681 tacatatcca ctaattgaaa gtacgattgt ctgttcttct aataattgat taacaagaaa
    22741 ataaataatg atcgttaaca gactaaggaa caagttctta ccaaaatgtt gctttattcc
    22801 ttgaaagaaa aaataaatga tatttcctga tccctcacct ttccattgcg ccattacata
    22861 aaaaacagca catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag
    22921 aatacctaat aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac
    22981 tattttcatc ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc
    23041 tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca
    23101 ggtccataaa aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc
    23161 atttttgaac tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc
    23221 gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa
    23281 atattaaact cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta
    23341 ataaaaaagg aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa
    23401 cctgcataag tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag
    23461 acttggaatg ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg
    23521 aaacgcatac gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact
    23581 gtccctccag caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa
    23641 accttaatat agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata
    23701 atgtcttttt cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc
    23761 aacaatataa ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga
    23821 ctattcacag taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat
    23881 ttgtttttct ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat
    23941 aaacaaaacc gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg
    24001 actgtaaata tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac
    24061 atatacttgg tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa
    24121 ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc
    24181 catttgagca gattcataca aagagggaga gattccctga atagcagtca agagaatcgt
    24241 catagtaaca ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt
    24301 tggatcgcga aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc
    24361 ccctacattc ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat
    24421 aactggaatg taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc
    24481 tactgctgct aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa
    24541 agaatgaatc aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa
    24601 acttacaaaa tttcttgatg taaagaaaat attcaaatct gtaaaactaa tagcaaatgt
    24661 tcctaaaagc ggtatgacag taaataataa taaaactaaa aaagatggag ttaaaaacaa
    24721 atacgcagca cgatcaggac gattccaata tttggtgaat ccgttcatat agtccacctc
    24781 atctttcatt ttgataaaat atacaatctt ggtttaaacg aatctgaata agatatccga
    24841 acaatcgctg tgatttttgc aataacacct atctgcaaat actaggtcga tcattcagat
    24901 atctgtatct tgccaacagt ctatcctgta taaagttctg tactattttt gcagtaattg
    24961 attgatggca tcatctgcat cgttcatgag tgtctcagga tcttctcctg ctaataattt
    25021 ttcaatcagt ggattgatga tatcgttatt gatcgcagag atttgggtaa aacctggtaa
    25081 atatggctct gcatagtcca tttgtttcga taattcagat acaataggat cattttttac
    25141 ttcttcatct tttgctactg aatccaagta ggcaggaaaa ccattttcga tactccattt
    25201 tttaccaatt tcagttgtat tccaatattt caaaaactca tagacagctt cttttttatt
    25261 attatctgta cttgccggaa tggcatatcc tacgccatct aaaatagctg tttttttgtc
    25321 tgaatcactc ccttgtggta aggtagtcac attataatca atctcgtttg ctttcaatcc
    25381 gttatttaac catggcccat tgatttcaat ggctagttga ccagcattca ttaaattgtc
    25441 catttcggcg ccagaaatcg attctggacc agtcttgtct acatggatca ttttttggat
    25501 cgcttttaac gttttaaggt tctcaggaga cgcaaattca gacttagtat aatcatcgtt
    25561 cactaattta ccgccatttg ctaacatcca gttatataac ggtgcagttc catccttatt
    25621 gaagacaaaa cctgatacat ttttactact atcagttaat tttggtgcca tttctgtcaa
    25681 ttcttcccaa gtagtaggtg gttgttcagt atctaatcca gcttgtttaa acaaatcatt
    25741 attccaatac atatacatgc cttgtacctg catggggaca aaatactgtt taccttctac
    25801 ttcacctaat tcaatagccg tttctttaaa atcagattta tctactccat catattccca
    25861 gaaatcgtct aatggttgta ccgctccgtt ttctacatac tgtgcaaaat ctggataatt
    25921 taatgcaata aagtcaggtg cttttttaga agaaatcgct gtaggaagtt tttcattcaa
    25981 attggcccaa gtcatgatat ccatttcaat tttgatgttt ttatcatttg ttttattaaa
    26041 ttcattaacg atttcttgca atatttcacc atcagatgca gtaaacccat tccaaaaagt
    26101 caaagtcact tctttatcag aatcagattc tgctgaactt ccgccaccgc aagctcctaa
    26161 aatacctgtt gcagctacca acaaagagcc aattaaccat tttttacctt tcattttttg
    26221 ttcctccttc atctgataaa cgacatctga ccacattcca agatagcggt ttcaataaac
    26281 aattgatttt cccatcttca gcttttacat ttgttgaatg acttggtttt acttgttcac
    26341 ttttaggaag attagttgct tttgtatcaa atccactcat ttcagaaaaa tcaatcactc
    26401 cctctaatga taattcagac aagtgaacag ttagattcat ttcttcttct ccacggttaa
    26461 ctgcaaatat gacaagttct ttgtcattta ttacggctat actttctaaa tacggcactt
    26521 cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg tgggcttaac acctctcctt
    26581 gtccatacac agcagcttgc ataaatggat aatagatcgt ctgtctccaa gcttctccgt
    26641 ttgtttctgt cctgattggt gcaataacat ttaccaattg agcaagacaa gcaatcttta
    26701 ctctatctgc atgtttcaat aaagtgatta atagacagcc taatagtaat gcatcttcaa
    26761 aattatagtt atcttctagt aaaggaggag caatctgcca aggcgctgct tgctcatctt
    26821 gttccgctga atgataccaa atattccatt catcaaagga caggttgatc tgcttcttac
    26881 tatgtttctt cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca
    26941 tgtctaagga acgtgcaaga taatgagaaa gatcattttt ttggttgcca taatattgat
    27001 gcagagacaa ataatctatt tgatcgtacg cttgatcaag aactgttaac tcccaatcac
    27061 caaatgtagg catttgccga ttcgaacttc cgcataaaac cacctcaatt gaatcatcta
    27121 cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc atattcttcc gctgttttat
    27181 gtccaatctg ccaaggaccg tccatttcat tccctaaaca ccatgtctta attccaaaag
    27241 ggctctcttg accattcgaa cgccttaaat cactccaata agttccttta ggaaaattac
    27301 aatattcgac taaattacga gctgcatcta ctccacgagt acccagattg acagccatat
    27361 tgatttcggc ccccacatgc tgacaccagt tataaaattc atgaatccca acttcattcg
    27421 tttcaattgt ccgccaagct aaatccaacc ttctaggtct atttgctatg ggcccaatac
    27481 tgtcttccca acgaaatcct gacacaaaat ttccaccagg atatctaatc atgggaatgt
    27541 tcaattcttt taccaagtga atgacgtctt ggcgaaatcc acttttatta gctgatggat
    27601 gatctggctg ataaatccct tcatatactg ctcgtcccat atgttcaata aatgaactgt
    27661 acaaccgcgg atcaactttg cctacacgct tgacactact aatgaacatt gtggcttcca
    27721 taactgacct cctttaattt atacatgaat aatacacctt gttgaaagcg ttgtcaataa
    27781 tataaatgtt aaacttactt attttttatc gattttaatt aaccaaaata ttaataatga
    27841 ttaaaacaaa taatttaata taacttttat taacaaatat gttaatatat aagaaaaacc
    27901 ttttaagagg tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa
    27961 gcattagcta gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat
    28021 gtcaaagatt tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa
    28081 aagctagagg aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc
    28141 cttcgtgtcg ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg
    28201 aaagaaacat cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt
    28261 ttagctacga tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct
    28321 cgaaggatgg atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct
    28381 aattttttaa atacagagga tacattggag atgttggaag tttctgtgga aatttcttct
    28441 gaatttcctt tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg
    28501 gagctaggaa catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct
    28561 gattggtatc cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa
    28621 cacttgacga atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa
    28681 gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc
    28741 tgtactttat ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac
    28801 tacagctgat atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg
    28861 acggctgttt ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg
    28921 tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac
    28981 ttcttttcct tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc
    29041 tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc
    29101 cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg
    29161 caaaatgtcg gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc
    29221 atgatctgtc cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat
    29281 acgttgacgt gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct
    29341 taatacttgt acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg
    29401 ctggataaag tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag
    29461 acgaacagcg ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt
    29521 tagttgcgcc aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa
    29581 agttttaaga aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat
    29641 ttcttctttt ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag
    29701 gctcatttat tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt
    29761 tccatgatag ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc
    29821 ggattcaagt ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct
    29881 ttgatttcgt ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt
    29941 aaaactgggc gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg
    30001 aacatgaaga tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct
    30061 tcagatttcc catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt
    30121 actttttctg cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg
    30181 atcgatacta gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt
    30241 gtcccaacac cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct
    30301 tcatccaagt cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt
    30361 tcccgaacac gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag
    30421 ttgattttgt cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac
    30481 gtatcataag tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat
    30541 gcttcataat catttccata agattggact aacgaatgag catgggtccc gctagctggt
    30601 atcccaaata tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc
    30661 gctctagttc cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat
    30721 agtggatctt ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg
    30781 aaattgacca tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc
    30841 acttggatta atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt
    30901 gctttaaatt caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca
    30961 cgcaagtaag caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt
    31021 tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca
    31081 aaaacagcat gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga
    31141 tatagatccg tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac
    31201 tcctcttggc aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa
    31261 atcttccatt ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact
    31321 ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa gaatacttat
    31381 tttattcaat gtatccgaga cacgaagaaa ttagcgtttt ctatttttct tacttgataa
    31441 aataagtaga ttttcctact acaatcgtag ccgttaagat accgatgatt gaacaaatat
    31501 aaccaaatgt ctctacaaca taatcaatgg caaaagtttt tgaagcaata atacgtcttt
    31561 ccttttttgc ttcttatccc caaagaaacg gatataactt attatgtatt gaattttttt
    31621 ggaaaggcga attatgacaa gatatacctt gcgttgaaaa aactcgcgga aaaaatatca
    31681 cacgttttta aaaagataca aagtaagaaa gcagctgtca atagaatgga aacaaaaatg
    31741 gcgagacatc aatcagtctc gccttgtttt ttattgtata cattagatat ggagcaggtt
    31801 accataaaga tgattattag tcgttattaa atagaatcct tcgtattttt ttgttctatc
    31861 actacaatat ctttatttgg acgtttgaat tgtattccgg cacgttctag ctcctgtttc
    31921 actcctacat ggactttgac atcttctcgt gtcattttta aaacgtgatg aatcgcatag
    31981 tcgatttctt gccttgtgat ttttttcttc gtattgatca tcacgacttg ctttttatca
    32041 tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa ataatttgta ataattaata
    32101 gccgtattcc caaaaaagaa ttccactact ttaggataaa atgtcttaat atttaaatta
    32161 ctattcacag gtgtttcgat tagcttcatc tcattcctct tttctatata gtcgatttag
    32221 atccaatctt aagttatgtt accatctttc ctagcattaa ggaatcggtt acaattgaac
    32281 ctttaaagac aaaaggattt accttttatc acttaatagt ataacataat ttcgtgagaa
    32341 aaaaatacaa ttttttcttg accatctcgt tacgtaaagg attatgctta catttgtcag
    32401 gaggaaaaat aatggagtac acaataaaga aaatgtcaga gatatcagga gtcagtccga
    32461 gaaccttgcg cttttatgat gagattggac tgctaaaacc agctaggatc aattcttcag
    32521 gttatcggat ttatggcaaa aaagaagtcg atcgtctgca acacatctta ttttatcgaa
    32581 caatggcatt caagttggat gatatccaag aggtcttaga caatccatct tttgaccacc
    32641 agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa acgggcacaa atcgataccc
    32701 tcttgacaac tgtccaacag acacttgata tgtacgaagg aggaagaaaa atgagtgaca
    32761 aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga aaatgaagaa tcctatggac
    32821 aagagattcg acaaaaatac ggaaaaaaaa aggttacagc atccaatgaa aaatggatga
    32881 atatggataa agaaacgtat gaagaaatgc aaactgttga aaagcagatg ttatttgatc
    32941 tcactcttta cttaaaagaa ccagaagatg aaagcttagc agaccgtatc ttccaagctc
    33001 ataagaagtg gttctcttac agctggcctg aatatcagcc aaaagcgcat aaaggactag
    33061 gaatgatgta cgaagccgat gaacggttca ctgcatatta tgacgagcga agtggtcatg
    33121 gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc gtaatctttt acatgatcca
    33181 acaaaaaagt acacgtaaaa tgacctaata acaaaacagc agacacccaa gttagatttt
    33241 tatgctaact tttggtgtct gctccattct ttcacaagtt ttttcatcct actttaaata
    33301 tattcatcgt tgaataaacg tataaggtat tttcacttta tccttttttt ctgtaagaaa
    33361 caattgaaat gccatttctc cgcaagcttc cagatggtgg tcgatggttg ggaaaccaag
    33421 aagctcgctg atcagcaaat tatcgcgtcc aatcaccact ggtgtcttat cttgcagata
    33481 gctttgcaag atgcctgctg caaccatatc cgaatttgta aagacaccat caactccaag
    33541 ctctttaaaa aagaagcctg cttgttttcc tttctctgca tctatacaat cccaaaaaac
    33601 aagtgattca tcaaaagatg gaaaaagttg atgacatagc tgcaaagtga tttttgagtt
    33661 ataacttagc ctgccacttc gccccaacgt gatgcccaat tgctttatac cttgctgctt
    33721 caaataagta agaccttctg taattgattg ttctcgatca ataaagacac agcttgcctg
    33781 ttttcccttg atttcttcac aaaaaactag aggtccatat ttttgatatg ccaataaatc
    33841 atctagtgta ttggcacgag acgtaatgat caatccatcg aaagctttag ctgcaaattc
    33901 ttcaagatac cttcgctctt tttctttatg atagcccgtc ggtaaaagtg tcactttgta
    33961 atcataatca aacgctgctt caatgatccc actgatcaac tgatcaaaat acggatgatt
    34021 ggtataaggc agaatcactc caatgctttt cgtttttcca taacttaact gtctggcatt
    34081 tgcattaggg atataatcga cttctttcat gaccgcaagt atttctttcc ttttttcctc
    34141 atctacatat ggatgtccat tgattacacg tgaaatagta gaaactgaat aacctgtgag
    34201 ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca cctatacaaa aagattttct
    34261 tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact gatacatttt tccttcatgc
    34321 gaaaaactga tccgctgccc gccccaatat atatcttttg tcaaattttc gatttcattc
    34381 attggtattt cttttactat attcctattc aacccttgaa aactgtacaa cgttaatacg
    34441 gatcccttga actcgacaaa cgcataaaaa taattgttcg ttgtgccatc tgttatctct
    34501 acataagtat tcattgcaag ccctcctttt gatactttga gtatagtgta tgaaacgcgt
    34561 ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat tcctctttca ttattaccaa
    34621 agtctcttat tttaaattga atacattata atgatactgt aaatatatct ttattattag
    34681 tgaat
//
LOCUS       DAYYNG010000012        34262 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000012 DAYYNG010000000
VERSION     DAYYNG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.012"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1287
                     /locus_tag="ACHBFA_000464"
     CDS             61..1287
                     /locus_tag="ACHBFA_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9393987.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNTNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTSDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(1375..1767)
                     /gene="rpsI"
                     /locus_tag="ACHBFA_000465"
     CDS             complement(1375..1767)
                     /gene="rpsI"
                     /locus_tag="ACHBFA_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9393988.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1783..2211)
                     /gene="rplM"
                     /locus_tag="ACHBFA_000466"
     CDS             complement(1783..2211)
                     /gene="rplM"
                     /locus_tag="ACHBFA_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9393989.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2745..3212)
                     /locus_tag="ACHBFA_000467"
     CDS             complement(2745..3212)
                     /locus_tag="ACHBFA_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9393990.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3332..4870)
                     /locus_tag="ACHBFA_000468"
     CDS             complement(3332..4870)
                     /locus_tag="ACHBFA_000468"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM9393991.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4950..5636
                     /locus_tag="ACHBFA_000469"
     CDS             4950..5636
                     /locus_tag="ACHBFA_000469"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9393992.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5837..6364)
                     /gene="msrA"
                     /locus_tag="ACHBFA_000470"
     CDS             complement(5837..6364)
                     /gene="msrA"
                     /locus_tag="ACHBFA_000470"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9393993.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6448..7125)
                     /gene="pgmB"
                     /locus_tag="ACHBFA_000471"
     CDS             complement(6448..7125)
                     /gene="pgmB"
                     /locus_tag="ACHBFA_000471"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9393994.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7122..8252)
                     /locus_tag="ACHBFA_000472"
     CDS             complement(7122..8252)
                     /locus_tag="ACHBFA_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9393995.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8252..10435)
                     /locus_tag="ACHBFA_000473"
     CDS             complement(8252..10435)
                     /locus_tag="ACHBFA_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM9393996.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10432..11457)
                     /locus_tag="ACHBFA_000474"
     CDS             complement(10432..11457)
                     /locus_tag="ACHBFA_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9393997.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11502..12329)
                     /locus_tag="ACHBFA_000475"
     CDS             complement(11502..12329)
                     /locus_tag="ACHBFA_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9393998.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12326..13393)
                     /locus_tag="ACHBFA_000476"
     CDS             complement(12326..13393)
                     /locus_tag="ACHBFA_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM9393999.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13419..14255)
                     /locus_tag="ACHBFA_000477"
     CDS             complement(13419..14255)
                     /locus_tag="ACHBFA_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394000.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14252..15121)
                     /locus_tag="ACHBFA_000478"
     CDS             complement(14252..15121)
                     /locus_tag="ACHBFA_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394001.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15191..16474)
                     /locus_tag="ACHBFA_000479"
     CDS             complement(15191..16474)
                     /locus_tag="ACHBFA_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9394002.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16488..18173)
                     /locus_tag="ACHBFA_000480"
     CDS             complement(16488..18173)
                     /locus_tag="ACHBFA_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9394003.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18448..19473
                     /locus_tag="ACHBFA_000481"
     CDS             18448..19473
                     /locus_tag="ACHBFA_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9394004.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20068..23721)
                     /gene="rpoC"
                     /locus_tag="ACHBFA_000482"
     CDS             complement(20068..23721)
                     /gene="rpoC"
                     /locus_tag="ACHBFA_000482"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9394005.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23800..27417)
                     /gene="rpoB"
                     /locus_tag="ACHBFA_000483"
     CDS             complement(23800..27417)
                     /gene="rpoB"
                     /locus_tag="ACHBFA_000483"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9394006.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28145..29113
                     /locus_tag="ACHBFA_000484"
     CDS             28145..29113
                     /locus_tag="ACHBFA_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9394007.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29131..30036
                     /locus_tag="ACHBFA_000485"
     CDS             29131..30036
                     /locus_tag="ACHBFA_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9394008.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30033..30839
                     /locus_tag="ACHBFA_000486"
     CDS             30033..30839
                     /locus_tag="ACHBFA_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394009.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30996..31964
                     /locus_tag="ACHBFA_000487"
     CDS             30996..31964
                     /locus_tag="ACHBFA_000487"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM9394010.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32281..32805)
                     /locus_tag="ACHBFA_000488"
     CDS             complement(32281..32805)
                     /locus_tag="ACHBFA_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM9394011.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33092..>34262
                     /gene="scm"
                     /locus_tag="ACHBFA_000489"
     CDS             33092..>34262
                     /gene="scm"
                     /locus_tag="ACHBFA_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9394012.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDST"
BASE COUNT         9782 a         7290 c         6419 g        10771 t
ORIGIN      
        1 cgccgcatat actaaaatta gtgaaatata ctgccttact ttttttaagg agtgaggatt
       61 ttgcctacaa agctttcaaa tgggaaatat aaaactaatc tgagatatcc aaaaaggttt
      121 aaagaaataa caggaattgc atcagaaaag tatcaaaaaa catttccaaa taggcaattg
      181 gcgattaaag ccgaaaatga tatgaagaag aaaatagaaa aagttttgag agaggagaat
      241 gcaaactcat tagaattaaa aggaaaaata acgtttaaaa aattttacga atctaaatgg
      301 cttcctagat atgaattagg tcaaacaatt agatcaaaca ggcctcctag tgatataacc
      361 attagtaata caaaagatat atttcggttg catattttac ctatgtttgg tgaatacgca
      421 atgaattatc taaataccaa tactgagata atatcagatg aattaaccaa aaaatcgaaa
      481 gaatatgcaa atataaaaat cataaaagga tatgttcgtt ccatgttcga tattgcagaa
      541 atactaaatt atatcgaatt taatcgaact actaaaatta ttcaaagtat cactgcacct
      601 aaaaaaaatg ctttagaaga aaaaagaata caagaaggta agcaagcact atcatcaaaa
      661 gaacttacta attggataga agcagtaaat gatgatttta ataatcattt acttactttt
      721 catgattaca cactttttat gattaccctg tatcttggtg acagaaaaag tgagacttac
      781 gctttgcaat ggaaatatat tgattttgaa aaacaaacgg tccgtcttaa acatacttta
      841 gataaatatc aaagaaaaaa gtttacaaaa ggtcgaaaag ataccgtcat tcaagtacca
      901 gaagttgtaa tgacattgct aagtgaatgg aaatcggtac aagctgatca attgttgaaa
      961 ttaaaaatta agcaaacgtc agatcagtat ctctttactt atactaaacc atctggagaa
     1021 gtaaattgtc ctgttcatgc cgattaccta aactatcgaa tcaattcaat taaaagaagg
     1081 catcctgact tagttcattt atctccacac aaactccgtc atacttatgc aactatagct
     1141 agacaaggtg gagcagatat gaatcaaatt tcaaatgctt taacacactc agatatttct
     1201 acaacaaaga tatatgtgaa cacaccagat atagttgata aggctgtttt tgaagctttt
     1261 cagagaggat tgaataagtg tgattgatat gtgttcttaa atgtgttctt aaaaacaaaa
     1321 gaagcacttt ccaaataatt gaaaagtgcc agaaacgttg atatataaag gatattaacg
     1381 ttttgagaat tgtgaagctt tacgggcttt cttaagacct ggttttttac gttcgaccat
     1441 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     1501 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     1561 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     1621 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     1681 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     1741 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     1801 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     1861 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     1921 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     1981 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     2041 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     2101 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     2161 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     2221 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     2281 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     2341 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     2401 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     2461 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     2521 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     2581 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     2641 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     2701 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     2761 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     2821 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     2881 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     2941 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     3001 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     3061 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     3121 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     3181 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     3241 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     3301 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     3361 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     3421 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     3481 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     3541 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     3601 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     3661 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     3721 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     3781 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     3841 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     3901 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     3961 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     4021 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     4081 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     4141 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     4201 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     4261 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     4321 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     4381 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     4441 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     4501 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     4561 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     4621 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     4681 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     4741 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     4801 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     4861 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     4921 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     4981 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     5041 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     5101 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     5161 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
     5221 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
     5281 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
     5341 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
     5401 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
     5461 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
     5521 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
     5581 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
     5641 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
     5701 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
     5761 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
     5821 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
     5881 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
     5941 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
     6001 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
     6061 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
     6121 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
     6181 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
     6241 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
     6301 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
     6361 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
     6421 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
     6481 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
     6541 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
     6601 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
     6661 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
     6721 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
     6781 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
     6841 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
     6901 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
     6961 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
     7021 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
     7081 tacaagaacg ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
     7141 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
     7201 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
     7261 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
     7321 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
     7381 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
     7441 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
     7501 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
     7561 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
     7621 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
     7681 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
     7741 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
     7801 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
     7861 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
     7921 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
     7981 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
     8041 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
     8101 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
     8161 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
     8221 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
     8281 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
     8341 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
     8401 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
     8461 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
     8521 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
     8581 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
     8641 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
     8701 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
     8761 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
     8821 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
     8881 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
     8941 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
     9001 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac
     9061 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
     9121 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
     9181 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
     9241 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
     9301 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
     9361 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
     9421 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
     9481 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
     9541 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
     9601 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
     9661 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
     9721 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
     9781 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
     9841 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
     9901 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
     9961 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
    10021 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
    10081 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
    10141 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    10201 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    10261 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    10321 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    10381 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    10441 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    10501 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    10561 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    10621 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    10681 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    10741 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    10801 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    10861 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    10921 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    10981 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    11041 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    11101 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    11161 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    11221 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    11281 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    11341 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    11401 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    11461 tttctctctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    11521 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    11581 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    11641 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    11701 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    11761 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    11821 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    11881 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    11941 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    12001 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    12061 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    12121 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    12181 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    12241 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    12301 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    12361 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    12421 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    12481 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    12541 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    12601 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatcccta
    12661 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    12721 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    12781 aatcggcacc gtgtttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    12841 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    12901 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    12961 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    13021 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    13081 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    13141 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    13201 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    13261 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    13321 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    13381 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    13441 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    13501 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    13561 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    13621 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    13681 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    13741 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    13801 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    13861 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    13921 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    13981 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    14041 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    14101 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    14161 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    14221 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    14281 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    14341 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    14401 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    14461 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    14521 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    14581 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    14641 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    14701 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    14761 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    14821 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    14881 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    14941 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    15001 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    15061 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    15121 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    15181 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    15241 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    15301 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    15361 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    15421 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    15481 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    15541 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    15601 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    15661 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    15721 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    15781 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    15841 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    15901 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    15961 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    16021 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    16081 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    16141 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    16201 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    16261 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    16321 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    16381 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    16441 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    16501 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    16561 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    16621 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    16681 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    16741 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    16801 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    16861 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    16921 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    16981 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    17041 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    17101 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    17161 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    17221 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    17281 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    17341 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    17401 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    17461 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    17521 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    17581 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    17641 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    17701 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    17761 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    17821 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    17881 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    17941 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    18001 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    18061 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    18121 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    18181 ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt
    18241 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    18301 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    18361 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    18421 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    18481 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    18541 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    18601 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    18661 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    18721 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    18781 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    18841 atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga
    18901 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    18961 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    19021 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    19081 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    19141 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    19201 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    19261 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    19321 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    19381 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    19441 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    19501 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    19561 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    19621 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    19681 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    19741 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    19801 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    19861 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    19921 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    19981 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    20041 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    20101 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    20161 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    20221 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    20281 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    20341 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    20401 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    20461 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    20521 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    20581 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    20641 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    20701 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    20761 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    20821 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    20881 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    20941 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    21001 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    21061 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    21121 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    21181 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    21241 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    21301 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    21361 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    21421 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    21481 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    21541 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    21601 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    21661 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    21721 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    21781 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    21841 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    21901 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    21961 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    22021 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    22081 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    22141 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    22201 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    22261 tggtttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    22321 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    22381 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    22441 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    22501 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    22561 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    22621 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    22681 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    22741 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    22801 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    22861 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    22921 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    22981 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    23041 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    23101 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    23161 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    23221 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    23281 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    23341 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    23401 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    23461 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    23521 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    23581 gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    23641 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    23701 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    23761 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    23821 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    23881 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    23941 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    24001 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    24061 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    24121 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    24181 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    24241 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    24301 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    24361 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    24421 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    24481 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    24541 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    24601 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    24661 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    24721 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    24781 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    24841 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    24901 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    24961 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    25021 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    25081 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    25141 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    25201 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    25261 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    25321 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    25381 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    25441 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    25501 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    25561 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    25621 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    25681 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    25741 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    25801 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    25861 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    25921 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    25981 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    26041 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    26101 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    26161 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    26221 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    26281 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    26341 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    26401 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    26461 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    26521 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    26581 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    26641 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    26701 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    26761 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    26821 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    26881 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    26941 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    27001 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    27061 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    27121 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    27181 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc
    27241 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    27301 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    27361 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    27421 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    27481 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    27541 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    27601 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    27661 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    27721 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    27781 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    27841 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    27901 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    27961 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    28021 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    28081 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    28141 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    28201 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    28261 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    28321 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    28381 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    28441 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    28501 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    28561 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    28621 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    28681 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    28741 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    28801 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    28861 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    28921 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    28981 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    29041 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    29101 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    29161 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    29221 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    29281 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    29341 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    29401 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    29461 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    29521 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    29581 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    29641 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    29701 tctaatggat gcattgctct ttctggctcc ttacttgcgc aaaacaacgg ctttgctgat
    29761 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    29821 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    29881 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    29941 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    30001 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    30061 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    30121 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    30181 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    30241 gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    30301 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    30361 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    30421 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    30481 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    30541 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    30601 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    30661 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    30721 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    30781 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    30841 cagaaagagg atgggacaaa aaatgaacgc ccaccctctt cttttttctc tttccttctt
    30901 tcgtttttgc tttatgtatc aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct
    30961 tcctgataga atggatggga aaggagcttg atgccatgac aacgaaagac aaggtacttc
    31021 aattattgaa aaagacacca gattttcttt ccggagaaaa gttggcacag caactggaag
    31081 tttcccggac aagtatttgg aaagccatca aagaattgga aaaggcaggc tatcgttttg
    31141 agcacgggcc tacagggtat cgttacctgc cctctgacgt actagatgca aaagaaatcc
    31201 agcaagagct acggtcgttt attcctgatt tgaaagtaca aacgatgttg acttctgaat
    31261 ctacgatgaa agatgccaaa ctggcagcag ctcaaggaga ttctgtttcg aagttatttg
    31321 ttgctgatac acaagaacaa gctcgtggac gttttggacg gccttttttc gctgaacctg
    31381 gacgaggaat ctacatgagt ctcttgctcc atccagacaa acggtttgat gaattgcctc
    31441 aatatacgat catcgcagcc agtgcctgtg tgaaagccat tgaatctgta accggtcgtt
    31501 ctgcttcgat caaatgggtc aatgatatct acatggatgg aaaaaaagtc tgtgggattt
    31561 tatctgaagc agtcagtgat atggaaagcg gcaggatcag tcatatcatc attgggatcg
    31621 ggcttaattt ttctgttcct caaaatcatt ttccagctga tctccaagaa aaagccatct
    31681 ctgtctttcc cgacggacaa gctagtgtca caaggaatca gctaatcagc aaaatctggc
    31741 aagaattgtt cacttatctt tctcacccag aaaaagcgtt ggacttctat aaagaaaaat
    31801 cattcgtatt gggaaaaacc gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg
    31861 caaaagaaat cggtgatcat ggcgaactga tcgtccaaac aaatgaacag aagatgagtt
    31921 tgtcttctgg cgaaatcagt ctcacatcta tccgtacgca ataattatca gatttcacaa
    31981 aaggacacct gatctcatac agtgtccttt tttctgaatt ctcttcaaaa gactctcctt
    32041 attctatcag ctgacagttt ctctatcctc tagaaaaagg tattctaaaa atatctataa
    32101 agcatggtac actttcttta aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca
    32161 cgatcaaaag agatagcagg ccgcttcctt tttcaatctt atagatagac catgggtaac
    32221 gataaaaaca aaaaagaact tgcgacttat tttgccgtaa gttctttttt agctagtgcc
    32281 ttaatctctt aatcccaaca aacgtctcgc atagatcacc tgaccagagt gcatagcagc
    32341 atcatcaata atagaaacga gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc
    32401 aattacttca tctagctcgg aaggttccag ccttcttaag taagaaatag caaaatcagc
    32461 agctcctcgc aagtatccta gcagatcttc tatttgatta gcttggatct gctttgcttg
    32521 ttccagacta tgcttccaat cttgttcgtt atctgctatc gtcatcggaa accggctttc
    32581 ccagtcttga ctatgccaaa ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa
    32641 ttctcgagcg gtatgccaag ccagccaagt cattgatttg atctgcgggg ctctatcttc
    32701 cactggaaaa cgattggcct cctcaataga gacgccttca aacaaacgtt caaaccgatc
    32761 gtatgcccga tccacactct cgcatagcat atctaaataa tccatttatt ttcctccttt
    32821 ttatttatcc tacttacttt ttcctaattc taggatacct acttatatgt tactacacac
    32881 ccttattttt tacactttaa cagtcaccat catcaatagt ttggagattg aaagaaaaag
    32941 atagtgtcga actatcaatt gaacaagaag caacaatatg aacgtttcat taatattctt
    33001 taaataaaca atgatttttc aaacgaacag tttaagacta ggagaaaaag agtataatag
    33061 gaaaatgttt gaaaggaaag gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct
    33121 agttgctttg ttgttcaata tcttttcgta tggtgtggcc gtttcagcgg aaacatacat
    33181 tcctaaagac cctgggaata tttataatga tctaactggt aactatggct tgttaggaat
    33241 tgctagtcaa ttccacgtat ttgctaaaaa taagacaact gtgaatgcac atacagacgg
    33301 aaatatagca acacatgaac ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg
    33361 tttattgaac caagaaataa attatataca aaaaactgat tctttcgcta attcttcagg
    33421 catacctaca ggtagcacaa gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc
    33481 acttcaagat ggtcatccta cagtcaatgg atccaggctt gatcacttaa ctccagatga
    33541 tttttataaa gatcgtgctg ggcatgaata tattgatttc gagcaggaat ttgctaaatt
    33601 gaatgtagca gcaaatgatc ttgcaacgat cactccagct aaaacatata ctgctgcaga
    33661 ttttccagac atgaacaatc ggacaatcga tttaactgat ttatcagata ccgggttttt
    33721 attggtaaat atcgatgcag aagtgttaac gatgaatacg ccacttcaaa tcattaatcc
    33781 aaatgatcaa gtagttgtat ttaatgtcat caacagttct tctgcattga acgtacagtc
    33841 accaatcaaa tacaatgatc gttcaaatca tgaaacagaa gatttttcgg atgcgaatat
    33901 ctcttggaat tttggaaatg agatgactga tttgacaatc agtgctcctt ttcaaggaac
    33961 aatccttgcg cctaatgcta ctatccgcat tactcaaaac caagacggaa cgattatagg
    34021 aacaaatgtg attttagatg cagctaccaa tcgttgggat cctaacgaaa tatttatcag
    34081 cgacaatggt acagatacga cagacacaag cgacagtaca gatacatcta ccacaagtga
    34141 tagctcagat tcatcaactt caagcgacag tacagatact acctctacca caagtgatag
    34201 cacggataca tcagcttcaa gcgacagtac agatactacc tctaccacaa gtgatagcac
    34261 gg
//
LOCUS       DAYYNG010000013        34206 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000013 DAYYNG010000000
VERSION     DAYYNG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.013"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACHBFA_000490"
     CDS             complement(251..1462)
                     /locus_tag="ACHBFA_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9394013.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1576..1782)
                     /locus_tag="ACHBFA_000491"
     CDS             complement(1576..1782)
                     /locus_tag="ACHBFA_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM9394014.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(1858..2604)
                     /locus_tag="ACHBFA_000492"
     CDS             complement(1858..2604)
                     /locus_tag="ACHBFA_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM9394015.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            2764..2988
                     /locus_tag="ACHBFA_000493"
     CDS             2764..2988
                     /locus_tag="ACHBFA_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM9394016.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            3012..3773
                     /locus_tag="ACHBFA_000494"
     CDS             3012..3773
                     /locus_tag="ACHBFA_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM9394017.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            3785..4042
                     /locus_tag="ACHBFA_000495"
     CDS             3785..4042
                     /locus_tag="ACHBFA_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394018.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            4134..4385
                     /locus_tag="ACHBFA_000496"
     CDS             4134..4385
                     /locus_tag="ACHBFA_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM9394019.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            4361..4540
                     /locus_tag="ACHBFA_000497"
     CDS             4361..4540
                     /locus_tag="ACHBFA_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394020.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEYRAKKASREGLTR"
     gene            complement(4660..5223)
                     /locus_tag="ACHBFA_000498"
     CDS             complement(4660..5223)
                     /locus_tag="ACHBFA_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394021.1"
                     /translation="MNYILYVLLVVYAIIYIKQIVNAVSEITTINYLLTTIEKVINDL
                     KRLNDHSGSYKSDPYFQNNVTPIREIMVKEMPRINKVLPYSLVTLSLNQSDDILEENF
                     RIIASQIFDKNNEIHYRKFWFLNPLIALKKFFLLPSEILSWFGINLSTFPGRIFSLVI
                     WVTSLFAQETISQILSFVITHFFKGQK"
     gene            5385..5609
                     /locus_tag="ACHBFA_000499"
     CDS             5385..5609
                     /locus_tag="ACHBFA_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394022.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            5619..5813
                     /locus_tag="ACHBFA_000500"
     CDS             5619..5813
                     /locus_tag="ACHBFA_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394023.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            5816..6838
                     /locus_tag="ACHBFA_000501"
     CDS             5816..6838
                     /locus_tag="ACHBFA_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM9394024.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            6801..7616
                     /locus_tag="ACHBFA_000502"
     CDS             6801..7616
                     /locus_tag="ACHBFA_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM9394025.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            7634..8416
                     /locus_tag="ACHBFA_000503"
     CDS             7634..8416
                     /locus_tag="ACHBFA_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394026.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            8416..9264
                     /locus_tag="ACHBFA_000504"
     CDS             8416..9264
                     /locus_tag="ACHBFA_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9394027.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            9261..9503
                     /locus_tag="ACHBFA_000505"
     CDS             9261..9503
                     /locus_tag="ACHBFA_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394028.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            9646..10014
                     /locus_tag="ACHBFA_000506"
     CDS             9646..10014
                     /locus_tag="ACHBFA_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9394029.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            10029..10190
                     /locus_tag="ACHBFA_000507"
     CDS             10029..10190
                     /locus_tag="ACHBFA_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9394030.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            10187..10513
                     /locus_tag="ACHBFA_000508"
     CDS             10187..10513
                     /locus_tag="ACHBFA_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394031.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            10510..11325
                     /locus_tag="ACHBFA_000509"
     CDS             10510..11325
                     /locus_tag="ACHBFA_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM9394032.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            11464..11775
                     /locus_tag="ACHBFA_000510"
     CDS             11464..11775
                     /locus_tag="ACHBFA_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9394033.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            11772..12017
                     /locus_tag="ACHBFA_000511"
     CDS             11772..12017
                     /locus_tag="ACHBFA_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394034.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            12014..12415
                     /locus_tag="ACHBFA_000512"
     CDS             12014..12415
                     /locus_tag="ACHBFA_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9394035.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEEQR"
     gene            12412..12969
                     /locus_tag="ACHBFA_000513"
     CDS             12412..12969
                     /locus_tag="ACHBFA_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM9394036.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            12966..13262
                     /locus_tag="ACHBFA_000514"
     CDS             12966..13262
                     /locus_tag="ACHBFA_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394037.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            13339..13752
                     /locus_tag="ACHBFA_000515"
     CDS             13339..13752
                     /locus_tag="ACHBFA_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9394038.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            14098..14472
                     /locus_tag="ACHBFA_000516"
     CDS             14098..14472
                     /locus_tag="ACHBFA_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394039.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            14702..15046
                     /locus_tag="ACHBFA_000517"
     CDS             14702..15046
                     /locus_tag="ACHBFA_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9394040.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            15051..15332
                     /locus_tag="ACHBFA_000518"
     CDS             15051..15332
                     /locus_tag="ACHBFA_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394041.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            15435..15749
                     /locus_tag="ACHBFA_000519"
     CDS             15435..15749
                     /locus_tag="ACHBFA_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9394042.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            15727..17421
                     /locus_tag="ACHBFA_000520"
     CDS             15727..17421
                     /locus_tag="ACHBFA_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9394043.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            17441..18619
                     /locus_tag="ACHBFA_000521"
     CDS             17441..18619
                     /locus_tag="ACHBFA_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9394044.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKDFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            18582..19268
                     /locus_tag="ACHBFA_000522"
     CDS             18582..19268
                     /locus_tag="ACHBFA_000522"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9394045.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            19268..20428
                     /locus_tag="ACHBFA_000523"
     CDS             19268..20428
                     /locus_tag="ACHBFA_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9394046.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            20438..21313
                     /locus_tag="ACHBFA_000524"
     CDS             20438..21313
                     /locus_tag="ACHBFA_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394047.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            21310..21621
                     /locus_tag="ACHBFA_000525"
     CDS             21310..21621
                     /locus_tag="ACHBFA_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394048.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            21611..21964
                     /locus_tag="ACHBFA_000526"
     CDS             21611..21964
                     /locus_tag="ACHBFA_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9394049.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            21954..22355
                     /locus_tag="ACHBFA_000527"
     CDS             21954..22355
                     /locus_tag="ACHBFA_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394050.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            22348..22752
                     /locus_tag="ACHBFA_000528"
     CDS             22348..22752
                     /locus_tag="ACHBFA_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394051.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            22764..23372
                     /locus_tag="ACHBFA_000529"
     CDS             22764..23372
                     /locus_tag="ACHBFA_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9394052.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            23392..23754
                     /locus_tag="ACHBFA_000530"
     CDS             23392..23754
                     /locus_tag="ACHBFA_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394053.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            23757..23939
                     /locus_tag="ACHBFA_000531"
     CDS             23757..23939
                     /locus_tag="ACHBFA_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394054.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            23956..27045
                     /locus_tag="ACHBFA_000532"
     CDS             23956..27045
                     /locus_tag="ACHBFA_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9394055.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNSISAT
                     ASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISN
                     AINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWS
                     NLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHF
                     KLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRI
                     LSSTSVSSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            27096..27833
                     /locus_tag="ACHBFA_000533"
     CDS             27096..27833
                     /locus_tag="ACHBFA_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9394056.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            27843..30134
                     /locus_tag="ACHBFA_000534"
     CDS             27843..30134
                     /locus_tag="ACHBFA_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9394057.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWILVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            30158..32356
                     /locus_tag="ACHBFA_000535"
     CDS             30158..32356
                     /locus_tag="ACHBFA_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9394058.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVITLDPNHKLEVLKPKSQPALLTGKTYTVSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPFQPNLLAEPYNMCREYPNENIADPTVKFPIESGDH
                     QIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTF
                     TPTNVVAMPKLFRVYQYPRSTVGACRIDWLKIEKGNTRTPNISEYKYRGTGMRDSNNP
                     KDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSKINSQLTASINEVDTTAKGA
                     QTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVG
                     ETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVSYGVD
                     ALQNRFAASGNIVTDLEVNDVLNMRTEIGVANGILQFTSCRTLVLTRLSD"
     gene            32434..32676
                     /locus_tag="ACHBFA_000536"
     CDS             32434..32676
                     /locus_tag="ACHBFA_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFM9394059.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            32692..32889
                     /locus_tag="ACHBFA_000537"
     CDS             32692..32889
                     /locus_tag="ACHBFA_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9394060.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            32900..33919
                     /locus_tag="ACHBFA_000538"
     CDS             32900..33919
                     /locus_tag="ACHBFA_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9394061.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGLNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            34109..34182
                     /locus_tag="ACHBFA_000539"
     tRNA            34109..34182
                     /locus_tag="ACHBFA_000539"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34143..34145,aa:Ile,seq:cat)
BASE COUNT        12066 a         5352 c         7168 g         9620 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttatata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta atagtttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ctaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gtaaagttcc gtcttgtttt gtatcaaata
      541 taaaatagtg tcttaatttc tttatattta atttccaacg aattttttta ttctctttga
      601 tcaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttcttccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggttttat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaataaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 ggcgtgtttt tttgtttata gactttcgaa aactaaagta gcttggattc tatcgccacc
     1621 accgaaacct ttactaccac cgtttgtagt ggaaatagtg tgtaatcgat aacctttaga
     1681 acactgtcta tttatagtgt tttcaagttc tgataggttt tgcgaccctt ttcctataaa
     1741 cttttctttt aatacaactt gcaatacaac gtaacttggc atattctttt ccttctttct
     1801 actatgataa taaatactaa cccctagccg gagcgccaac tacgactggg gtatttttta
     1861 gaatacgact ttcccgatta ttctaatctg ttccggtgag acgactctat cttcatataa
     1921 ttcgtttata gagcgtagaa taatcgtttg actctcataa tccttatata cttctttaca
     1981 cgtcacaccg tcaccatcta tttcaacgat agcaatctca ccgtcttcca cgtctggctg
     2041 ttccttaaca aatacaaatt gtccattcct gataactggt tccattgaat cgcctataat
     2101 aggaacagca aaatcagcat tgtttggaac tctttcaaat tcttcttcct ctacaactgt
     2161 atcgccataa actaattcag tcgggttggc ggcagaagct gaatttttga ttgtcggaag
     2221 tttttccttt ttaggaaagt gcaccacgtt tcctcgattt tgctcttcga gttgctgttc
     2281 agcaaagttg tagacttttg tttgtcttgg tttttctaat tgattgtaga ttgtttcaat
     2341 ggatggaatg ttatttttct tatcttcttt aggaacatcg taaccaagca accatgcctc
     2401 actcacattt aaagttttag ccaacaaata aagcttgtgt tggtcgggcg atgacttacc
     2461 attgacatat tgcgatagat gactttttga catttttatt ccagtttctt tttgcaaagg
     2521 taaagacata ttcaaaatat caacttgctt taaatttctt tcagacatta actgattaag
     2581 tcgttgctga gtcgtttgct tcatcttata cctcctttct gatacttatt atatacctta
     2641 ttgaacataa gttcaataaa aaagttaaat taatttgaac ttttatgttg acaatgtttt
     2701 atgcaggagt tatactttgg ttacaaagtt caaaacattt gaactagaaa ggagcgaact
     2761 aatatgagtt ttgattattc taagttgtcg ggaagaattg ttgagaaata tggaacgcaa
     2821 tacaattttt caatcgcttt aggtttgtcg gaaagaagtt tgtcattaaa gttgaataac
     2881 cgagtaccat ggaaaagcac cgaacttcaa aaagccattg aattgctaga tattccaaaa
     2941 gaagaaattg gcgaatattt ttttaattta gaagttcaaa atgtctgaac ttaagaaagg
     3001 aattcgaaaa aatgaacaca caaaaatttg aaaacaatct atttgaatta gaagttaaga
     3061 cggaaaatgg tgaatcgtta tttaatgctg aaacagtagc aagaagtttg ggtttcgtgg
     3121 aaacaaaaaa cggtaaagaa tatgttcggt ggagaactgt caatggatat ttaaaaaaat
     3181 acctttcgca agaagttgcg aaaaacgact ttatcagtga gccaatggtt tacaaattag
     3241 cctttaaagc aaacaacgta cttgctgaaa aattccaaga ctggttagca acggaagtcc
     3301 tacctcaaat ccgaaaacac ggcatgtacg caacagatga actacttaac aatccagact
     3361 tgctgattga agtagcaacc aaactcaaag aagaacgcac gttacgattg atagctgaac
     3421 aacgagtaaa tgaattacaa ccgaaggcag actattacga ccgaatcttg aacaacaaag
     3481 gattagttac agttagtacg attgctaaga actacggtat gagcgctgta tcgtttaata
     3541 aattgctaca tgaattaggt atccagttca atcaaagtgg cacatggctg ctatacagta
     3601 agttccaaga caaaggatac acgcatattg agccatttga ttatgaagat aaaaacggca
     3661 atcggcaagt aaaaacaagg atgaaatgga cgcaaaaagg acacatcttc ttatatgaaa
     3721 cattgaagaa gaataattac ttaccgatga ttgaacgtga acaaacagca taggaggtaa
     3781 cactatgaac gaggaaatga gccaaacaat caaggtacaa aaaatgatag atgatctaac
     3841 acatgcaatt gatagccaag ccgataaaat catcaaggaa ttgcaaggac aaaaagtttg
     3901 ggatgcaaaa atgcttttca agactatcaa atttgaaatg aatccaacaa aaagaaagct
     3961 cgccgatgtg ttggaagaaa agttggcttc cgctatcaac gagcaagaat tactatttga
     4021 aaccgatact tttaacagct gatgttttat agatgacagt ttcatccgaa tctatatcat
     4081 tatagattcg attattgcaa tgtacgaatt ataaatacag gaggaaataa ccaatggagc
     4141 aactagcttt agttaacctc tcagacctta aagcttcttt ggcagaatct gagattgcaa
     4201 atgaagtgtg ggatacaaag caagctgcgg aatatttgaa aaccacgaca cgcacgctaa
     4261 ctaaagatgc cgaatctgga aagattccag cagcgaaagt tggcagagag tggaggttta
     4321 gtagtatcgc tttgtatcag tatctaaaag gaggaaaaac atgggcaaat tcaacagagc
     4381 actagtattc agcgcaccgc taatcatcta cgctttaggg ctttggggaa atagacaagc
     4441 gttgatagga acgattgttt acatggtttg gatttttatg gggcttgatg aagctgagta
     4501 cagagcaaaa aaagccagtc gggagggact gactagatga gaaacaaaaa aatatctcta
     4561 acgctattaa ctatcatcac gattgtcgta tcgcaactat tctttttaac tatattaaac
     4621 gacatatcga tgtcggaccg attgttggtt caatcagctt tatttctgac ctttgaaaaa
     4681 atgggtaatt acgaaagata gaatttgaga aattgtttct tgggcaaata acgatgttac
     4741 ccaaattaca agactaaaaa ttcttcctgg aaaagttgat agattaatgc cgaaccaact
     4801 aagtatttcg ctgggtaaga gaaaaaattt cttaagagca atcaacggat ttaaaaacca
     4861 gaattttcga taatgaattt cgttgttttt gtcaaaaatt tgagaagcga taattctaaa
     4921 attttcttca agaatatcat ctgattgatt caaggaaagc gttacaaggc tataaggcag
     4981 aactttgtta attctgggca tttccttaac cattatttct ctaataggcg tcacattatt
     5041 ttgaaagtaa ggatcagatt tatagctacc actatggtca tttaatcgtt tgaggtcatt
     5101 tatcactttc tcaattgtag taagtaaata gttaatcgtt gttatttcag acactgcatt
     5161 aacaatttgc ttaatataaa ttattgcata aacaactaga agcacataca aaatgtagtt
     5221 cattgaaaac accaccattt ttttacctaa attataccaa aaggagagaa gaaaaaatgc
     5281 aagaattagt aattttgaaa aataaaaaaa gacacgaacg ccagcaagca tgatcgtgtc
     5341 caaaagaaaa atcttttaaa ccattataac acaggaggaa ataaatgatc tcagttaaag
     5401 ggttaggcga tgaaatattc gaagcaatga tgagcaaagc acaacaggac atcaaagaaa
     5461 aaatcatttc tgctgcagcg caaggacaaa ctagctgcac tgttcgatcg agaggaacga
     5521 caccaagttt tcttttggcg ctagaagatg aaggcatttt gaatatcaaa cgaagagatg
     5581 gcagcgtcag attgttttgg caattttagg aggccgtgat gaaagatttt gattctttag
     5641 gcgccagaca gcaaccacca aatgaagcta gtcctgttgg agttgattgg caagaaaatc
     5701 cgttatatcc aggtgatact tgctatctaa cagaagaagg ctatgtacca gtagacgcaa
     5761 ttctagagta cgtccagcaa cattatccaa agattgaatt aggaggaatt taggaatggc
     5821 aaatgattta acacaaacaa cacaacgttc attagatgag caggtaatca gtaatcttgg
     5881 acggttacaa gaacaaggtt tagaaatgcc accaggatat agtccacaaa atgcgttaaa
     5941 aagtgctttt ttcgaactca ctaacaatac aggaggaaac ttgttgcaga tggctgcaaa
     6001 caatcaagaa atgaaaacct ccatttctaa tgcactttta gacatggtta ttcaagggtt
     6061 atctccagca aaaaaacaat gctatttcat taaatacgga aacaaggttc agctaatgcg
     6121 ttcatatttt ggaacaatgg cggtccttga tcgtgtaact ggaggggcag atattacgcc
     6181 tgtagtcgtt cgacaaggtg atgagtttga agttgcaatg gatggaccaa acatggttgt
     6241 caaaaagcat gaaacaaaat tcgagaattt agacaatgaa atcattgcag cctatgtagt
     6301 catcaagtta acaaatggta aagagaccac aacagtcatg acgaaaaagc aaatcgatca
     6361 aagttgggct aagtcaaaaa tgaaagattc tggaccgcaa aaggaatttc cagaagaaat
     6421 ggctaaacgg acagtaatca atcgggcagc taaaacattg atcaacacaa gcaacgacaa
     6481 cgatttatta gttcaagctg caaaagatac gttagaaaat gaatttgaca atgatcggaa
     6541 agatataacg ccacaaacag aaaaagtggc tactctcgaa caaaaattct tttcaaacaa
     6601 aaagattact gaaccaatac aaaaagaacc agatccgatt gtgattccag atgatatcca
     6661 aaatgaagta actcgagttg ctgatgtacc tgcccatcca gaaatagagc aagcacattt
     6721 gatcgaaaat gaggatatca atccaattca agaagaattg atagatattc cagactttgg
     6781 acgcgaggaa ggtgtagaag atgtctcaga atttgaagac gacgagtacc ctttctgatg
     6841 agaattatta ctccaatgaa gctgactggc agtacatgtc aacatcacaa tataagtcct
     6901 ttttaaaatg tgaagccgca gccttagcaa agttgaaagg cgattgggca ccgacgtcgg
     6961 atcccaaagc cttactcgta ggaaactacg tacattctta ttttgaatca aaagaagtac
     7021 atgaagcgtt caaagaagaa aataaatcca aaatgttttc tagtaggaag ccgtatggat
     7081 tgttgaaaga tttccaaatt gctgaacaaa tgattgaacg acttaagcaa gaagaagcat
     7141 tcatgaacat ttatcaaggt gagaaagaaa cgatcgtgac tggggaacta tttggaacga
     7201 catggaaagg caagattgat tgcttgaatg ttgaggatga gtattttgtt gatatcaaaa
     7261 caaccaaaga catgcatgag cggaaatgga acgaaaacta cggatcaaga gaaacgttca
     7321 ttgtcaattt tggttacgta cttcaaatgg caatctatca agagcttctt ttacagcaat
     7381 acggaaagat gttcatgcca atcatcgctg cggtatcgaa gcaaacgcct agtgaggcga
     7441 agttgataac gattgatcaa gataagatga gctacgagct aatcatgcta aaagaaaata
     7501 ttgaaagaat tgtcaaagtg aaaaatggcg aagaaaggcc gataccttgc ggtacgtgcg
     7561 agtattgtcg aggtaacctt tcaattactg gattcactag catggacgat ctttaaaagg
     7621 aggtgctttg cgaatgaatc taggatacat caagttatac cgtaaagtga ctagttcatt
     7681 cgtttggacc aattccgata tgttcaagct atggatattg tgtttaatga aggctagtca
     7741 tgaagataga aaatttttat ttaacggtca agaagtacgt ttgacaagcg gacaattcgt
     7801 cacaggagcc catgcaatag caaaagagta caacgaagga gtcccgagtg acaaagcaat
     7861 tgcatggcga acgctatgga gatggcttaa gaaatttgaa aatgaagaat tattgacaat
     7921 ccaatcgaac gctagatata gcgttataac aataataaat tggtctgact atcaatcgag
     7981 tgacaagcca gtgacaagcg acggacaatc gagtgacaag ccagtgacaa caaacaagaa
     8041 tgataagaat gataagaatg ataagaataa taatcctcgc aactctcgaa aaacacgaga
     8101 gtatgcagat gacgatccaa ataaaaaatt ggccattctt ttattaaaac tcattcgaaa
     8161 gaatcgtagt ctcaaagaac ccgatttgaa caaatgggcc aatacgattc gtttgacgat
     8221 tgaagctgac aaacgaactg gaaaagaagt tcaagacatg attgtttggg ctactaacca
     8281 tgatttttgg tccacagtca tttcatcgcc aactagctta agaaaaaatt tcgatacaat
     8341 ggtgggtcaa aaaaataaaa gaaagcaaca aaatatttct aatgatgagt taccagaaac
     8401 aggtgaggat tggtaatgaa taaaaaacta agtgcaatgg ctgcacctta cggcggatta
     8461 aaaacagcag atcataattg cccgaaatgt ggtgatccat tgtatatctg gaaaacaaaa
     8521 aataaagatg gtactgatcg atgcggtcct acatgtatca ataagatttg tggttatcga
     8581 gaaatggtaa ctaaaaatca aaaagaagct atccaaaaag cgaatgaagc aatgaaaaag
     8641 gacgctatca atcgaatgct taacagttca atgattacag atgatgccat atggaccttc
     8701 gattttgatg gatacaaagt agttgatcag gaaacagcac aaataaaagc gatggctcaa
     8761 gaatgggcta aaaaaattgt aaatggtagc acgattcatg cagtgataac cggtagaaca
     8821 ggagccggca aaacccatct aggcgctgca gtgattaaag aagtcatgag ggcatctaat
     8881 tataaaattg cttgttcatt tatcagttat cgagaacttt tagaacagtt gaaatttgca
     8941 atgaatgatc cagaagcaag aaaagccgta acaggttcgc tgatggctga gataaagaaa
     9001 tcagatttcg tggtaatcga cgatttgggt gcagaactag gaagaatgga agaaaacaac
     9061 caggcaacgc cttatgatgt tgatgttctc acatcgctca cagaagctcg tctaaacaaa
     9121 gctacgatat tcacaaccaa tttatcatcg aagcaattaa aacacgcgta cggtgagcga
     9181 gtgttctctc gtgtaatgaa tggaacgaaa gggaacatag ctgtattcaa aacaacgaca
     9241 gacaaaagga ggaatccagt ttgacctttg tagtgaaaaa aatgtgctac ttagatagcc
     9301 gagggcgagg agaagctagt gtcgagtttg ctaaacacca cacgactaaa gaagaggctg
     9361 accttgttgc aagtgtttgt ggcggcgaag tagtcgaagt tattaaaccc aaaagacgat
     9421 tttcaaaacc taagacaaag ccagtgaaaa aagagtgttg ttgtccgaaa agtaaccaag
     9481 catggatgag aggtgcaaaa tgacttgttt aaaatgcaac gatgaaacag ttatttggta
     9541 taagacattg cttggatggt caacttgtga accttgtcca atatgcaatg aaaatggacg
     9601 acgttcgaaa gaacgactcg aaagactaaa gaaggagtat agcaaatggc aacaagaagc
     9661 aaatacggaa acaaaaagca cgaagtagac ggcatcactt ttgattctat cacggaagca
     9721 gagtattacg atctagcctt gtggcaagca caggcgaatg gttggaaagt gaagctccag
     9781 gaaaggtttg agctgatgcc gaagtttgaa ctagagggca agaagtatcg caagatcgag
     9841 tatattcccg acttcacatt ttacaaaagc ggcaagctgg tcaaagtcgt agatgttaag
     9901 gggatgcaga caaaagactt taagatcaag gcaaagttgt tctgtcatca atatcaagtg
     9961 ccgttgatat tagccaaaaa atatcggaat acgttcaagg aagagcgttt ttaacgaggt
    10021 ggtccatcat gacaacagaa gaagtgattc aaatgcgaat tcgaagcctt cggcgtgaga
    10081 ttgacgatct ggaacgaaca aaggcagtga tggtcaatga aacggctaga aaggcaatcg
    10141 atttgcacat agagaattta agaagggaaa tccgtagatt ggaggaatga gcgtggataa
    10201 ggaagcggct tggcgaaaat taatgttgct gattcaagat gagaactggc aagaagatga
    10261 agcagtggtt gctgaagttc agcgtctagg aaagattgct aacggacgta tacgaaaaaa
    10321 gccagacaaa agaaaacagc gcaaagggaa aatcgtcgtt gttttacacg aaggcaaaat
    10381 tttgatgcaa ggaacagcta gtgagctgtc tgcagaaact ggatatacgc gtgggactat
    10441 tcgaacgtac gcttggcgaa atcacaccga tcgaaaaggg cacgaatata agtatttgga
    10501 ggaagaaaaa tgaacgaaaa caaattaatc aaattgggtg tagcaggagc agtaatcgta
    10561 ggtattggag ttatcggagg atttaagttc ttcgaaaaaa tcgataatgg atatgtgggt
    10621 gtgcgctatt caatgaacgg cggtatcaaa gatgaagcac tgacgcaagg tgtgaaattt
    10681 gtagggattg acaaagtgat ccaatatcca attcgcttgc aaactatcca atcaaaaaat
    10741 atttcagtat ctacaagcga cggcaaaaag acaacgattg atatcaaata tgactacaaa
    10801 gttgattcaa ctaaagcagc aaaaatgtac aaggaatttg ggaatatcac ttcggaagat
    10861 atcgaaagtg gatggttaaa atctaagctt caaaaggtcg ctcgtgaagt ttatgcgaaa
    10921 tatagtctgc ttgatgtcct ttcaggagat tcctctaaag ttgaagctga ggtattaacg
    10981 aactttgcta aatcagttga atctaaaggg tttgaagtcg aagacgtaac acttggtgtt
    11041 ccagatgtcg ataaagaaac acaaaaatca atcgatgcga tcattcgagc tggtcaagaa
    11101 aatgaaaaag cgaagctaga cgcagaaact gcaaaaactc aagctgatag tgaagcttac
    11161 aagaaaacaa aagctgcaga agcagaggca gaatctaatc gcaaagtcgc cgaatcagta
    11221 acagacaatt tgattcgtta tgaagaagct caagctcgca aaaagcatgg atgggtaaca
    11281 gtaaatggag cagatactgt agttacggat gaagcaggca aataatatgg gattctttat
    11341 ggctaaaatt ctcttgttct taggtttagt aggagcagca tatctcgtgt atgcgctcct
    11401 ttccaaaact gatgacaaag aagatgacaa caacgatgaa atgaaatttt aggaggagaa
    11461 ataatggacg aactaatcac aaaagtagag cagtgggcta aagacaaggg actggatcaa
    11521 gcagatccaa aagcacagtt tttgaaagta gctgaggaat tcggggaaat agcttcggcg
    11581 atggcaagaa gtaatgatga actatttaaa gatagcgtag gagacgttat cgtcacgctg
    11641 attatccttt ccatgcaaaa agggacaaac gtacaagagt gtttagaaat ggcatacaac
    11701 gaaatcaaag gacgcacagg gaaaatggta gatggtgtat tcgtgaagtc gagtgatttg
    11761 gagggcagca aatgataaaa aagctcattc aattcagcat ggatttatat gatatcgaat
    11821 caggagcaac actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc
    11881 agattatttt gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag
    11941 aatcaaaagc ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg
    12001 aggaggacag cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag
    12061 agatgtagcc gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat
    12121 aaatcctacc gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg
    12181 aatgaaagat aagaatggtg tggagatatt tgaaggggat gtagtaatta catcaaaagg
    12241 agcgattgga tatgttgcgt atcttcaaca agaagccggc tttgtagttg tactaaagaa
    12301 aagtgattat agattaggcc ataggaatac tggcgaaagt tatgatgtag caactgctca
    12361 tgaagtcatc ggaaatatat acgagaatag cgagttattg gaggaacagc gatgaataaa
    12421 caggaagcaa tcaaaaagtt agaaagtatt aaagcgatag gaaatgatgc aatagctgct
    12481 tgctataacg agagtataaa ttcaggtatt acgttaatga aaaaaataga cgaaccgcag
    12541 aaaccagtta ttccacaact cgtggccggt tggcttgaga aatctacgga cccttttaca
    12601 aaagctgaaa aaatagcgta tttaatcaaa tctaaagatg gtgattcata ttatttctgt
    12661 gattggtttg tacgagatgg catagtgacg caagagcaag gagaagaatt actcgcttgg
    12721 gcaacaagac aatcatatga aacactattg agcctataca acggctacga ggttgagaaa
    12781 gagccgttat gggcaataaa gaatgccgat ggaaactatc ttactaaatg tgctttatgg
    12841 ggaaaagatg gagtaaatta tagttttgaa tgcaatccat ctcatcgatt gcttttcact
    12901 gataaagcaa cagcggatgc tgcagcattg ttggtgaatg gaacagtgga agaggtggta
    12961 gaaagatgaa actaaaagac ggattttacg ctagcagtca cggtatcggc ggtttaatgc
    13021 tagatatgcc gacaaagaac cctaaaacac gcaagaaatc aaaattcaaa gtcggtgaca
    13081 tggtccgctg tgaagcagaa gagttcgttt atccgttcag aggatacgtt aaaaagatac
    13141 tgtcaaactc agcaatcatt cgtattgaaa atacaatgaa gtgtgatcgt tcgactgcaa
    13201 aacacaagca ttatttagct gtagcgagat tagttgacat ggaagtaatc aagagcaaat
    13261 aaaaaaagcc ggatcgctcc gactgattca ataaatccaa cacatttatt atatcacata
    13321 aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt
    13381 aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg
    13441 atagatatta agtcgccgat tattacggat atgccgaagg caccaaagca cggcaataag
    13501 gcagaagacg cgatcattca gatgatggat atagaagcgg agagagatgc gattctagcg
    13561 gctttgatgg ctcttagtct aattagtcgc cagatactct actacagctt ctgtgtgcca
    13621 gatagcttct caaactacag gattagccgt gaagtgggtt attcagaaag aagtatacaa
    13681 cggatgaagt cggaagctct aatagagttt gcagaagcat ataaacacgg aagaataatt
    13741 gcttataaat aatttggcgg ttttttggcg gaatgatggc ggtttttagc tatttaccag
    13801 tgatattatg gtagtgtcga aagattagtg ataggtctaa gacaaaataa aatgtaaggg
    13861 aggaaatctc cctcatcgtt taattaagct tcgatagaca gcagcaaata aactaaagga
    13921 tgtgggggtc agctcctaca gttagttcat gtgttgctgt ctattaattg caactctttc
    13981 ggttttattg agtatttact caacttaaaa aaactgttgt ttgtagatgt aagctgttcc
    14041 tatttgtaca tagtaaaaaa tgatataata aaaatattac gaaaggggtg agagtgcatg
    14101 gaagacaaaa tgtatttaag acaaaaagtt ttcacaaaaa aagatattga aaatcaaatt
    14161 aatggattta tacagaaatt agaaaatgca aattcttatt tgataaataa ggaactaaat
    14221 aaagcttatg accagtggaa agaagtatat gatgagttga aattgattca aaatgaaaca
    14281 aaattagtac gaacggagaa aaagaatgaa aatagtttct tttttgatgg atatgctata
    14341 ttgatgttgg aaactgtagc aaaacaaaat ataaaagctc ccaaaaaaga actatctgac
    14401 aatattgata acgcattagc cgagttaagg tattacgtta tgcaaataaa agatgttagg
    14461 atcactcgtt gagtggtctt tttattttgc acaaaggagg taacaacaat gtatagacca
    14521 caatacttag aacagaagta tgaagtaatc actgtgcaaa atggtaacgg agagatagta
    14581 cgaaagtata gaagaccaat aaagagcgat acatataaac gaaaggaaag caatgaagtt
    14641 attccattgt atggcaaaag aatagctaag cattaaataa gattgtgaaa ggagacggga
    14701 catgaacgag gaattctata gatggctatt acagttgata agagaagatc gtttggttaa
    14761 gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta
    14821 tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat
    14881 aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg
    14941 tgttgaacat cataatgaag ttcatggcag atatcttaca gcattggata aacaagagaa
    15001 gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca
    15061 atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg
    15121 gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata
    15181 actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg
    15241 tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat tacattgact
    15301 tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt
    15361 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
    15421 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
    15481 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
    15541 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
    15601 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
    15661 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
    15721 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
    15781 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
    15841 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
    15901 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
    15961 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
    16021 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
    16081 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
    16141 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
    16201 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
    16261 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
    16321 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaaatcga
    16381 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
    16441 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
    16501 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
    16561 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcaaattt aaaacggaaa
    16621 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
    16681 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
    16741 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
    16801 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
    16861 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
    16921 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
    16981 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
    17041 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
    17101 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
    17161 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
    17221 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
    17281 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
    17341 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
    17401 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
    17461 ggtaggatac ttcacaaaaa aagcaacggt tactttagaa gaatactttt gtaaattgca
    17521 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
    17581 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
    17641 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
    17701 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
    17761 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
    17821 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
    17881 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
    17941 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
    18001 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
    18061 gatgacatca caatttaagg attttatgga agctgataat gcaggtgcgg tatttcaatt
    18121 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
    18181 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
    18241 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
    18301 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
    18361 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
    18421 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
    18481 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
    18541 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
    18601 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
    18661 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
    18721 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
    18781 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
    18841 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
    18901 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
    18961 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
    19021 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
    19081 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
    19141 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
    19201 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
    19261 caaaataatg acaattaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
    19321 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
    19381 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
    19441 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
    19501 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
    19561 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
    19621 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
    19681 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
    19741 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
    19801 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
    19861 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
    19921 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg
    19981 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
    20041 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
    20101 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
    20161 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
    20221 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
    20281 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
    20341 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
    20401 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
    20461 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
    20521 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta ttttttttga
    20581 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
    20641 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
    20701 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
    20761 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
    20821 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
    20881 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
    20941 taaccgtcct aaagcgaatt attggaaaaa ggtttatctg gattctaatc ggctatatcg
    21001 ctaccttaga gggcaaaaaa caaatataca aatttggaca gccgagatgt gggcacggct
    21061 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
    21121 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
    21181 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
    21241 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
    21301 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
    21361 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
    21421 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
    21481 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
    21541 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
    21601 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
    21661 gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac
    21721 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
    21781 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
    21841 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
    21901 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
    21961 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
    22021 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
    22081 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
    22141 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
    22201 gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
    22261 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
    22321 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
    22381 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
    22441 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
    22501 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
    22561 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
    22621 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
    22681 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
    22741 ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca acgttcgata
    22801 acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca atcgcaacga
    22861 attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag
    22921 cgacagaagt aaaatcaaaa tctaaaccaa tcaatatgac ggtaacaatt actgcacatg
    22981 taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt attaaaccag
    23041 gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct gcagagatcg
    23101 tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact tcgaatacag
    23161 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    23221 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    23281 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    23341 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    23401 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    23461 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgact
    23521 gtgggaaaag gatccatgga tatttctgtt attcaaggtg caaaggctgt gtatattgct
    23581 taccgccaag cgaacatgac tgattatatt tcattcgatg aatttatcga taaatgggat
    23641 tttgatatgg ctactgccag ctatatttat caattgatga tgttcaaaca agcacgcgat
    23701 gcttatcaaa aagaatttga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    23761 caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    23821 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    23881 acaagagtgc ttatgaagga tggaaaaact atgcaataaa ccaatctcag aaaaactaaa
    23941 gaaaggaggt aaaaaatggc taaaaagaga acagaagcag aagtaacttt catagctaac
    24001 gatgacggat tgaaatctac gttaaaagaa atcagtgctg aattaactaa aaatagagca
    24061 gaattaaaac tagaacaagc tcaattacaa cagactggtt ctgaatcaga caagttagga
    24121 agtaaattat cttctttaga aaagcagtat gaattacaaa gccaaaaagt tgaagtaact
    24181 agccaacgtt tagccaatgc caaaaaatat tatggagaaa attccaccga agttcagaaa
    24241 cttgagagag aactgattaa ccaacaaaca gcgcaacaac gtttgtcaaa cgaaattgat
    24301 aaaacgagta atacactagc tcaagcaaaa ggcgaaatac agacgtacga gtctacaatg
    24361 caacagttgg atagtgaaca aaaaaatgtt caagctagtg cttctctgat tgaatccgaa
    24421 tacaaaaaat ggcaagtaac tgctggtcaa tcagcttctg aatccgagaa attagcgaaa
    24481 gcccaagaat atgtttctca acaatctgaa aatgcagaga aaacgataga tatcctgaga
    24541 cgacagttag aagctacaca gtctgagttt ggcgctacat ccacagaagc aatgcagatg
    24601 gaggcgaagc ttaatgatgc tgaacgtgaa tttgaagagt taggacaagc tgctaaaaat
    24661 gtagatacaa ctaacttgga cgatatcgga agcaaaatag atatgaataa tttaatggaa
    24721 gcttctgacg ttttaagcga cattggcgat aagcttacag aattagggaa acaagcagtg
    24781 gactctgcta acagtgtagg tagttcccag agtaaaatac aagctaattt tggtttgact
    24841 aaacaagagg ctgaagaatt aacgaatgta gccagagaca tttattataa aggttttgga
    24901 gaatcgttag atcagtccac agatgcattg attttggtaa agcgtaattt aggcgattta
    24961 aataatcaag atttacaaaa tattacggaa caagctatgg tcctagaaaa caccatgggc
    25021 gctgatatgg atgaaacgtt acgtggtgta aatggcttaa tggtcaactt cggattgagc
    25081 gctcaagatg caatggattt aatggtttcg ggtactcaaa acggtttaga taaaacgcac
    25141 gaattaggcg acaatatggc agaatatagc caattatgga gtcaaatggg atattcagct
    25201 gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg gtgcttataa ccttgataaa
    25261 gtcaatgact tagttaagga aatgggaata tcgttaacag atggtcgatt tgagcaaaac
    25321 atggatatgt ttagtgaaag tactagaaaa gcttttgaag agtggaaaaa tggtggagga
    25381 acacaaaaag acgttattaa ttccatgatt caagatttta gcaatatgga tggtcaatac
    25441 gaccaattaa ataaagcttc tacaatttgg tctgcacttg gcgaagataa cgcgatgaaa
    25501 gttgtccaat ctttgactga tgttaaccat acatttgatg atgttagtgg atctgcacaa
    25561 aaaatgaatg aagattctac tactccgtta caagaattaa acggaaaaat agctgaatta
    25621 aaggattcat tagctcctat aggcaacaca atcatagatg cactcgaacc agtaattgat
    25681 tttctaggaa agatggctga tgcgtttaat aatcttccac aaccagtaca ggattatgcc
    25741 gtagcgattg gcggattgac tgctgcattt actttattaa tgccaataat agttggcttc
    25801 atggctctag gtggtcctac tacattaata ataggagcag ttattactgc tattgctgga
    25861 gttatagcaa ttataaaaaa ctggggcgca attactgact ggtttaaggg aatatggagt
    25921 aaattcactg attggttggg tggtacttgg gaaagcataa aagaaggtgc ctcatcagtt
    25981 tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga aatttggcaa
    26041 ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc ttccaacatc
    26101 tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa tatttggaac
    26161 tcaattagtg caacagcttc tagtatttgg aatagtatca agaatacaat cactagcttg
    26221 gtacaagcag ctgctacagt aattcaaaat atttggtcaa ctgtatctag ttggttaggt
    26281 ggaatttgga attcaatcag ctctacagca tcaaatatct ggagtagcgt gactagtagt
    26341 ataagcaatg ctataaacgc agctaaaagt gccattcaaa gtgtttggaa tagtatatct
    26401 tcgtggatca gcggaatttg gaacggtatc aaaaacactg ctttgaatct ttggaatgga
    26461 attacaagca ctattagctc taaagtaaac gatggaaaaa atgcaatttc aagcggttgg
    26521 tccaatctaa caggtattgt ttccgacata ttcaataatg ttaaaagcac gatagctaat
    26581 atatgggaag gtatcaaaaa gactgttagc gctccgattg attggattag agataaaatc
    26641 agtagtgtct ttgataattt gaatatttct ataccacata ttccgttacc acattttaaa
    26701 ttgagcggag aattcaatcc attgaagggg aaaatcccaa cattgggtgt tgattggtat
    26761 gcgaaaggta gtgtgtttaa ttctccaaat attatcggtg taggtgaatc aggacctgaa
    26821 gcagttttac ctttgaaaag atctgtgctg caagaaattg gtgatcgtat cttgagtagc
    26881 acctcagttt catctaggac acaagcggtt caacctgtga acaattacga attcaatttc
    26941 acaattgatg gtaacgcaga tgaagtcact atgaagcaaa caactcaaca aatcattgat
    27001 agcattacaa aagttcaaaa tgataatgct tcggcatggc gttaaacagg agagtatttc
    27061 tcctgttttt ttagtattaa aaaggatgtg aaaaaatgac tgattgtata cattctataa
    27121 tcgatggatt tcctgattat ttgcataaat tggctttagc tgaaagacca accatacctt
    27181 ctccaaaaag acagagagtt gaaacttctg ttttaggaag gttaggtggc ttagtgcaag
    27241 attactcgtt tgaagacatg tcgtttacat tgcactataa ctatttagag gatgtggaag
    27301 accatcaagc gttcaagcaa tcgttttata tcatgcgtca ttggttaaac tatgcaaaga
    27361 aattagaatt ctctgacgat cccaacgtct attacgttat ccagactatc gatattgggg
    27421 atgcagaaaa cgatattgtt gaatggggag agttcgatgt aaatattact gcgaaaccat
    27481 tcgcaagagt tcaagaagat gtacctataa ccgtagataa accacagtca tttaacttgc
    27541 tgaataatag tttagaagaa agttttccaa agattatcat cactccttca gctacttcat
    27601 gccagttcat cttaaatgat tatgtgttta gtttcgaagg cttagtagca ggaactgatg
    27661 tagtcattga tagtgatttg atgctttgct acgaagagca atcggacgga gatattttag
    27721 atcggtccaa caaaatgaag accatgcaat atccgacatt gcaagtggat atcaatcatt
    27781 ttaattgcac tggtttgagc aaaatacaaa tttatcgtaa cgggttaagg taggtgaaat
    27841 agatgatcga taatttaata actatttacg ataaaaatga cgcgaataat ttagctgaac
    27901 atttatatga tacgcaaggt ttaggcgctt tgtcagactg gttaacagct actgttagca
    27961 ataaactaaa cggagccgag atatttcagg gtacttatcc aataagcgga actaatgcag
    28021 atttgattgt agaaggacgt attattcagt gttatgtaga tgaaaatcga gcaaaacagc
    28081 gtctacggat ttattatgca aaaacttctg taataggcaa tacgatagaa gtaaaagctg
    28141 aacctatttt caatgatata agaaaatcgg tgttgaataa atatgacagc ggaacagaaa
    28201 aaatcactgc tactcaggca tggcaaaacg caaaagcttt agcgaaacca gctatccctt
    28261 cgcagttttc tttctcgtca ttagtagata cgcttgctaa tgtgaagata gaaaaagcga
    28321 attttttaga attctttggt ggaaaagagg gatctattct agatcgattt catggggaat
    28381 ttcttaaaga taataacaca ttacatcatg aaaaaaggct aggtacggat cataaaatca
    28441 aagcgattta tactaaaaat ttaactggtc ttgacttaga gatagatgct caaagcgttt
    28501 tagttggagt ttatccattc attagcagtt cttcagaagg agaagacgag atcactctac
    28561 cagaagaagt cattttcacg gattacgtgg atgattatcc tgctggatat gtttcttttg
    28621 ttgattttaa agacaaagcg actgatgtag ccacattaag ggaagctgct aaagactggt
    28681 tgaaaacaaa catagataaa caaaaaccac aagtgagtgg ttcgattgaa ttagtaccat
    28741 tgaggcatca aagaggctat gaaaaatttg ttgatctaga aaaagtttcg atgggtgacg
    28801 gagtagatgt gtatcatcca cagttaaaag tgaatatgtc agcgagaatt gtggaatata
    28861 cgtttaatgt tctaaccaat tcatacgata aattagttgt aggaaacgtc aaaacaaact
    28921 tcttagaaaa cacagagaat aatgtaagca atttgattaa tgatgccatt gatcaattga
    28981 aaaatggtgg cgaaatcagt gatttaatca atgatattgt agatcatcaa actgatatga
    29041 ttactggtca aaatggtggt tatgttttat tagatcctaa agaagcgcct agtcgtattt
    29101 tgattatgga cacaccagat aagaataccg caaggaatgt tttacaaatc aacaatgctg
    29161 gtattggttt ttctaaaact ggcattaatg gaacatatga aaccgcatgg acattagatg
    29221 gcggattcaa tgcctcgttt attacggctg gtgaaatagt aggaattact attagaggta
    29281 ctacattaat tagtgatggt gctgattata gaacaagtat tgctaatggc aaaatgactt
    29341 ggtactcaaa aaaagttaac aaagatatta tggagctaga agcacgtgat tatgtaagtg
    29401 ctgatgccgg tattgtatca tacaccatga aaactggtgg tggtttcatg attagaaatc
    29461 cacagggtaa cttggttttt agtacgtggg ataatggtaa taacagaccg tttctatctt
    29521 ttggtgcgcc caatttcagg tatagcaatg ctagttatgt aacttctggc gacggtagtt
    29581 ctttaagcat taatggtagt gcgggtaact catgggaatt taaggtagct ggtaggacta
    29641 tgaaatttac tagtgatggt atgctaacgt taccaggttg tttttttggt tcatgggaag
    29701 atgggaaact tgctaggttt gaacaatcaa cggtacaagt atataaagat tttactgtta
    29761 gaggtactaa aaactcaact gtaccaacag aacattatgg acaacgacta ttgaacgctt
    29821 atgaaactcc agaatattat ttcgctgatt atggggaagc cgttacaggt gacgatggta
    29881 aagttcgtgt tgatattgac cccatgtttg ctgagacagt aaatctaagt cggtatatga
    29941 cacatgtgac acctacagaa ctagttttgt gtgctgttac tcatgaagat attgaccatt
    30001 tcatcattga aactagtaag ccaaacgtat tagttagatg gattttagtg gcacaccgtc
    30061 tagggtatga agatattaga ttaaaagagg atacagcata tgatagcaca gtgcttgacc
    30121 aaaaacgttt ttaaaacgaa gacaaggagg tatataaatg gctagcagtt tatataattt
    30181 ggctttagat ttcagcaaag aattaaacta caccaaagct attatggctc gtcagggtga
    30241 taaagggatt acggtgacgg ttaaaccata tttaaatggc ttgcagatgg atacgagtgg
    30301 cggaacattt actttaaaag gaacaacacc atctaaccgt tacgtagata gtgttgcaac
    30361 tagcgtaact agtgaagaag tcacgttttc tcttgatggc acatttatga gtgaagcagg
    30421 atattataaa cactgctacg tagaatatag aaaagacaat caaattttaa caacgcaaga
    30481 tatcattttt ttctcactag gagtgtctga catttcgcaa ggtcaagccg atgaatatgt
    30541 ttcgcaatta gaagagttga ttcgaaagta taatgaaact tttgatgctt ttatggctga
    30601 aactaaaggt agagtggata gcttaaatca acagattact gatttaactg gtcaagctaa
    30661 aacgctacaa gacaagttag atgctctgaa agaagaaatt tctaagttag gtaacttaca
    30721 agtgatgtac agtaacagca ttgatttcgg gggctatgat tattcgggga atccgaattt
    30781 gatggctaat ataaacgctg atagcttctc gcaaggtagt ggtgccttat ctgttgtaga
    30841 tgatggtgac gaggtagtga ttacgcttga tccaaaccat aaattagagg tattaaaacc
    30901 gaaaagtcaa ccagccctat taacaggcaa aacatatacc gtgagtgtag aaattatgtt
    30961 agaaggtgat ttcactggag accctagcaa gataggccta agatatatta aaatgcctaa
    31021 ctgggtatca gagctatata cgcgtaatac attaactgct actaagggtg tatggcaaaa
    31081 actaactggc accgttaaaa ttactgctgc gagtgataac gctgaaagct ggcttataat
    31141 gctacaaaat aaagacgcta ataacagcct atccggcaaa ctacgtttga gacacgctaa
    31201 actcgaagaa ggctcaacag ctacaccatt ccaacctaac ttattagcag aaccttacaa
    31261 catgtgtcgc gaatatccta acgaaaatat tgccgatcct acagttaagt tcccaatcga
    31321 atctggcgac caccaaatat atcaaggtta cacagaagaa gagcttatga taggtcaaac
    31381 gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa acctttgtag cgtataatca
    31441 ttggactgct cgtttaggag aactaaagcc ggttgatggg ttgacagacg tatggtctct
    31501 aacattcaca ccaacgaatg ttgtggcgat gcctaaactt ttccgtgttt atcagtatcc
    31561 acgatcaaca gtaggcgcat gccggatcga ctggctcaag atcgaaaaag gcaacactcg
    31621 aaccccgaat attagtgagt ataaatatcg tggtactggt atgcgtgatt caaacaatcc
    31681 aaaagattat gtttgggatc tagcaccaga atatgtcgaa gataatttgg ccacagatat
    31741 taaaatttct gaaattactg gtaaagcaaa caattatacc gatgggaaag tatcgaagat
    31801 taattcgcag ttgactgctt caattaatga agtagacacc acagctaagg gtgctcaaac
    31861 aaaagcgaat gctaatgcga ctgctataga tgaattagac aataagatcg atgaacgcat
    31921 taatgataca gctactaccg cattttatgg cgcacaaggt gaagtctcta acaaaacgaa
    31981 agtagcggag tatggagtag gaacagggta tactactaca gctgtaggag aaacacattt
    32041 cgaacgtcaa tctaacggat acgttaaatg cttaaaagct ggtaagtatt caattagtgc
    32101 gcaaatacgt gtgcaattgg gcgggaaata tgcttcatgg ttatatactg atttatataa
    32161 agatggaact tctgttgata atttagtatc ttatggggtc gacgccttgc aaaatagatt
    32221 tgcagctagc ggaaacattg taactgattt agaagtaaac gatgtactaa atatgcgtac
    32281 tgaaatcggc gttgcaaatg gaattttaca atttacatct tgccgtactt tagtcttgac
    32341 tagactatct gattaataca atatttataa ttttgctatc aacacgctca aaggagggtg
    32401 ttttttattt tgcaatgaaa ggaggatagt tggttgaaag acgaagcaat acaagacgtg
    32461 gtagaacgct tagtgcgtat tgaaacgaaa ctggataatt acgaattatt acgcgaaaaa
    32521 gcggaaagtg caaaagatag agcggatcag gcatattcta ttgcgcttaa taatgcggaa
    32581 gatatcaaag aaatgaaagc caataataaa tggtcgtggg gttacatgat cggtttaggc
    32641 attacgatca ttggctattt cttgactaaa ttgtaaagga ggtgagaaga aatgatttta
    32701 cccgataagt attatcaagt cattaaatgg acggttttaa cagtattacc agctgcatct
    32761 gctttagtag ccatgttagg caaagcctat ggatggaatg gaacagatat gacagttctg
    32821 actatcaatg cagtagccac gtttttaggc gttatcactg gtgtgtcggc ttataatttg
    32881 aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt actgcgatga gcctattaac
    32941 ggctcttttt ttattgccaa ttaatgggtt cgcctatacg attaacaatg aatttaattt
    33001 gggcctaaac gaaggtagct cacaagtagc aaataatcag tacattttac tgcatgaaac
    33061 ggctaatgaa acagcaacag gacgcaatga agcgcaatat atgcaacgtt catggactag
    33121 tgcttatact gcttatattg tgggagacgg tggaattgtt taccaagttg gacaacctgg
    33181 ttatgtacag tacggtgctg gttcgtatgc taatgctaac agtcctgtgc agattgagtt
    33241 acaacacaca cataataaag caacttttga aaagaactat aaagcatacg ttgaattggc
    33301 gagagattcc gctaagaaat acggtattcc gcttacattg gacactcctt ataaccaacc
    33361 aggaatcaaa tcacatttat gggtaacaca aaatatttgg ggcgatcata cagatcctta
    33421 cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta gcatatgatt tagctcatgg
    33481 atttaccgat gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc
    33541 tggtgcagct aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg
    33601 ggaaatcgaa aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga
    33661 gtatattttc attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg
    33721 aatttataga ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt
    33781 atctttcaat atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa
    33841 tgatccagag ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt
    33901 aaatattcct aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac
    33961 aactataaaa attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata
    34021 aatagtcaca ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat
    34081 tgtttatggc tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg
    34141 ctcataaccg tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa
    34201 tcgttc
//
LOCUS       DAYYNG010000014        33088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000014 DAYYNG010000000
VERSION     DAYYNG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.014"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..484
                     /locus_tag="ACHBFA_000540"
     CDS             254..484
                     /locus_tag="ACHBFA_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9394062.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL"
     gene            484..825
                     /locus_tag="ACHBFA_000541"
     CDS             484..825
                     /locus_tag="ACHBFA_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9394063.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQLKSLDFKERRLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            1520..3601
                     /locus_tag="ACHBFA_000542"
     CDS             1520..3601
                     /locus_tag="ACHBFA_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9394064.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAERLAAEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFSLKLENDKQVRLETEIKEVKDGFLNRRERIFDLVLAIAYS
                     RNPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFFHYFPLAEIKKIEEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDYMITEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHIPFHLKEEALFENLYKHMAA
                     LIIRIKHAIQVMNPLKENIRTNYFPIHRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYQSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVDLVFSTFQLNYRNKPLLVIDPILTETNILVIEDFLKRNEELQRLKPNSHESTQLFT
                     QIIGVMEESGGRVDRIIYGKLEKLFEINNLEINKREIQPMLKDILTDSNIMIKEKAAT
                     WEETIERAAKPLVKENIVEERYVEAMIRAVNEYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NVEDFKQLLFQ"
     gene            3637..3939
                     /locus_tag="ACHBFA_000543"
     CDS             3637..3939
                     /locus_tag="ACHBFA_000543"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9394065.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFSVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            3965..5254
                     /locus_tag="ACHBFA_000544"
     CDS             3965..5254
                     /locus_tag="ACHBFA_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9394066.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEEIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPILKAF"
     gene            5273..5680
                     /locus_tag="ACHBFA_000545"
     CDS             5273..5680
                     /locus_tag="ACHBFA_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9394067.1"
                     /translation="MFGFFKKKENEPKKRSIKEEKILSPEVTEKIQSEITQLNQEIST
                     TQEKPKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDVMRQIAKQVTIKGNK"
     gene            5703..6572
                     /locus_tag="ACHBFA_000546"
     CDS             5703..6572
                     /locus_tag="ACHBFA_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9394068.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKGTFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            6574..7194
                     /locus_tag="ACHBFA_000547"
     CDS             6574..7194
                     /locus_tag="ACHBFA_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9394069.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SVGFNNLEERAKQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKTRLQELAERY
                     GYQVMTIRLTADFEVLWQRRYKRDREPDRHLSYIMDHYHYGDILEDRSKGTNHITKEA
                     FKKIITDRKYDEFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            7391..7996
                     /locus_tag="ACHBFA_000548"
     CDS             7391..7996
                     /locus_tag="ACHBFA_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9394070.1"
                     /translation="MNNKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTVIEEGHPTPKVDVRAYIRKDGKILLIEDKRTREWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVESTQLQAVFDTNKQKDIPQLFQYYKLVFACTIHDEDAKFI
                     ENNETSDMGFFSIEELPKLSEKRTTKKQLLILENKNQLYCD"
     gene            8132..8584
                     /locus_tag="ACHBFA_000549"
     CDS             8132..8584
                     /locus_tag="ACHBFA_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9394071.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLKKVCKNVSEDWNFVKKGNKRIY"
     gene            8621..9117
                     /locus_tag="ACHBFA_000550"
                     /pseudo
     CDS             8621..9117
                     /locus_tag="ACHBFA_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9135..9845
                     /locus_tag="ACHBFA_000551"
     CDS             9135..9845
                     /locus_tag="ACHBFA_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM9394072.1"
                     /translation="MKKVIVDANFWEVFPEATIEILSVSGIDNHVTEENEEMYHQLLN
                     SAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKISGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVSIVRHTLTKDESEAEL"
     gene            9987..10409
                     /locus_tag="ACHBFA_000552"
     CDS             9987..10409
                     /locus_tag="ACHBFA_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9394073.1"
                     /translation="MESVYRQERKSTRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGIFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMDEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            10429..10653
                     /locus_tag="ACHBFA_000553"
     CDS             10429..10653
                     /locus_tag="ACHBFA_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394074.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRARNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKVKSHY"
     gene            10897..12210
                     /locus_tag="ACHBFA_000554"
     CDS             10897..12210
                     /locus_tag="ACHBFA_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9394075.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALSGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAMLLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGKLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            12538..14175
                     /locus_tag="ACHBFA_000555"
     CDS             12538..14175
                     /locus_tag="ACHBFA_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9394076.1"
                     /translation="MERKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDVIWLSPVYESPNDDNGYDISDYQAIMAEFGTMEEMEELIEEAKKRNIRIVMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGEEPNGLRSIFSGPAWQLDEQTGQYFL
                     HLYSKRQPDLNWKNKQVRQAIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSDPKRKELSMIFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDLYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDISEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEETPEHVFAYSRIYEDQRWLVVCNMSSEKQVFTVPRE
                     ALRIIVSNYPLKQLPDGKVELRPYEAFVMEVNKEKLI"
     gene            14323..14757
                     /locus_tag="ACHBFA_000556"
     CDS             14323..14757
                     /locus_tag="ACHBFA_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9394077.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRLLNKLADEAFRKTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FTKEVDGSWEELHQSYKAVLGNEASKELAEIMNEATDKLQKK"
     gene            14860..15951
                     /locus_tag="ACHBFA_000557"
     CDS             14860..15951
                     /locus_tag="ACHBFA_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM9394078.1"
                     /translation="MSQFDQSIILPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIVQIF
                     HGGRMTSRATLSGEQPVSASAVAAERPDAETPRALSENEIKETIQAFGEATRRAIESG
                     FDGIELHGSNTYLIQQFFSPHSNRREDQWGGSLENRYRFIDELLTTVFSTVEKYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRETKLDYLHVSLNAYDRRARSDKYSDQSI
                     LEAVHDTIQGKIPLIGVGGIRTRKDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            15981..16658
                     /locus_tag="ACHBFA_000558"
     CDS             15981..16658
                     /locus_tag="ACHBFA_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM9394079.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYKDEDFRNRALGNTLRPDDVDAKDYAAVYYAGGH
                     GTMWDFPEATDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGADYQSGPAFSEVVQIDGRLLTGQNPQSAHQLGEV
                     VVEALKH"
     gene            17003..18298
                     /locus_tag="ACHBFA_000559"
     CDS             17003..18298
                     /locus_tag="ACHBFA_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9394080.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLIPTSEIYLIEKQSKLGWIPSGF
                     NLIVKGKVTSEKQLSWITKEELIDRYRIHVYTEKTVIGLEEKKVVLAGGDKLDFDRLI
                     LATGSNQNFRNVPAEARFIHPVKKIQNIAALQQKIMDAKKIAIIGAGQAGIEIAEGLA
                     SGKTQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGISLFLEEQVVSLSENETAVVET
                     ERRRENYDLVLLANHSRPNNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSATKDQGTYRPVGNQWFGYFLGSVGMTEE
                     ESIFYPQKIETGYAEYKLAAINQSKVKVKFLFDSEKKILGAQLQSRKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPNMVWSKVANTAYED"
     gene            18288..19748
                     /locus_tag="ACHBFA_000560"
     CDS             18288..19748
                     /locus_tag="ACHBFA_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394081.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKTSF
                     DQYLEDIELIGRMMGKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLGRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYETLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLEKLQETAMQQLPRKSTEDSALSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLNRELRGVSGIEIESYEEGKNYDLVITF
                     CQRNKQQNEYYLSEFASPYDIIRLKRRIEMLKKEKN"
     gene            19885..21381
                     /gene="glpK"
                     /locus_tag="ACHBFA_000561"
     CDS             19885..21381
                     /gene="glpK"
                     /locus_tag="ACHBFA_000561"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9394082.1"
                     /translation="MTEPTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQDILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEAAA
                     KRSTNEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            21395..23218
                     /gene="glpO"
                     /locus_tag="ACHBFA_000562"
     CDS             21395..23218
                     /gene="glpO"
                     /locus_tag="ACHBFA_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9394083.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQAAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQGIAPHIPKPDPM
                     LLPIYEDEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYEGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LADIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVRELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMTPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAEYFGWTEEEKVQQTKRLEALIAESDLRELKGEK"
     gene            23219..23935
                     /locus_tag="ACHBFA_000563"
     CDS             23219..23935
                     /locus_tag="ACHBFA_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9394084.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGSISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            24241..26169
                     /locus_tag="ACHBFA_000564"
     CDS             24241..26169
                     /locus_tag="ACHBFA_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9394085.1"
                     /translation="MNNEMISQLQFDQIKKEIQARAIGNYSKKRISKMTTSSNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLVLSPADLIEYADFLRSSRMI
                     TKFFDKNQYQAPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQII
                     EKEIQSKLLKFLRHPKNKEMIQEAIIVQKGECYTIPIKASYKNKVDGTIIDESNKGTT
                     VFIEPTVVSKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEAIDLLIETMTVLDIIF
                     ARAKFSREINGITPKINKSEHIVIKQGRHPFLPDHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            26295..26849
                     /locus_tag="ACHBFA_000565"
     CDS             26295..26849
                     /locus_tag="ACHBFA_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM9394086.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(27579..27893)
                     /locus_tag="ACHBFA_000566"
     CDS             complement(27579..27893)
                     /locus_tag="ACHBFA_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM9394087.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(27907..28167)
                     /locus_tag="ACHBFA_000567"
     CDS             complement(27907..28167)
                     /locus_tag="ACHBFA_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9394088.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            28337..28651
                     /locus_tag="ACHBFA_000568"
     CDS             28337..28651
                     /locus_tag="ACHBFA_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9394089.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            28653..30308
                     /locus_tag="ACHBFA_000569"
     CDS             28653..30308
                     /locus_tag="ACHBFA_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9394090.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            30391..32526
                     /locus_tag="ACHBFA_000570"
     CDS             30391..32526
                     /locus_tag="ACHBFA_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9394091.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            32701..33036
                     /locus_tag="ACHBFA_000571"
                     /pseudo
     CDS             32701..33036
                     /locus_tag="ACHBFA_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11111 a         5608 c         7130 g         9239 t
ORIGIN      
        1 tagatgaact agcaagtttg attgcagact gtagacaaag gtatcccttg cctctttacg
       61 aagagtgtag gttgttactt ttgtctattt ttgtatagaa atagaaaaca tttgtctcca
      121 tttccaagct gctaattttt caaagtgcac catttctcaa aatgcttttg atcatttctc
      181 catctagttt acaaagggta cttttgcgtt acttagtgtt gtgtagtata cttttactga
      241 agaggtgatg atgatgcttg ttacaaaatc tagattacaa ggaagttctg tagtggttac
      301 tttaccttct gataacggaa agaaaccttc agaaaaccaa gaatatatcg ttgtatattc
      361 tgacgatggg acaattacgt tagttccaaa aattgaagat ccatttagtg gtggagaaga
      421 ggccgaatat tatgaaaaag acgaatggga agatttaact cctgaaggga gagaaaggtt
      481 atagtgtcag aagaaagaat ttatattcct aaaaaaggtg atattgtgtg gattgatttt
      541 gatccttcga taggaaaaga aattcaaaaa agacgaccag gactagtcgt ctctcgttat
      601 gagtttaatc gtaaaacaat gttttcggtc atatgtccga ttacttccac gataaagaat
      661 ttaccgacac gttactcatt accagaagag ctagatataa aaggacaagt acttatttct
      721 caattaaaat cacttgattt taaggaacga agactcaaaa aaatagaaaa cctgccattg
      781 caagacatgg ctaaaattga tcagattata caatatatgt tttaagcgtt gtaactaaac
      841 tctacaatat ttttatatgc taaacttaac aaaatttttt gacatttaaa aagaaaagtg
      901 acaataaaaa atagcatcta aatagcaatg acccccaaat ttaactcttg gggggacatt
      961 tcaaaaggga taagaaaaca aacattctcg tcgtatctga ctagtgaaaa agatggcagg
     1021 ccgattttat tcagccgatt atgctggatt gacaggaaat atttatgaat ggagctgtga
     1081 caaaaatttt gtcgcagctc ctttttctga ataaacggtg ttccagagat agcctatcgg
     1141 caataaggca cgattttgta aaatatggga agctattttt caaaatcgct tcttatcgct
     1201 caaggctgaa cctctccgtc acaacctttc cactaaatgg tattcaaaag cttacattta
     1261 cggtaataag ctcaaccaaa cgaaaaatag gaagcactat ttttgtttgg ctgttcttct
     1321 tacttgatgc aggacagctt tttcatgacc tcttttttgt aataataggc gtatgtcttt
     1381 ttcgttgaga agttgaaaaa gcatacggct tattttttgt ttgctatgct tactttcttt
     1441 ccacttcaag tggcagaaat atctactttt tattatgtaa gcggaaccat atacttggaa
     1501 tcagaaggag atcatttcta tgaaaacaag aacaattgaa atacttcaca gaatcatgac
     1561 agcccaatat ccagtaaaag cggaacgtct agcagctgaa ttcaatgtca gtcagcggac
     1621 gatccgtcag gaagttcttg aagcgaatat ctggttgagt agtaaaaagc ttccggaaat
     1681 cagaacaatt cgcaacaaag gtttttcgtt gaaactggag aatgacaagc aagttcgatt
     1741 agaaactgaa ataaaagagg tgaaagacgg ctttttgaat cggcgggaac gtatttttga
     1801 tttagtttta gccattgctt acagccgaaa cccgatccat ctaaacaaaa aagaagaaga
     1861 atttcaaatc tctaaaagta cgttagatga agatatgaga cgattacgag caacactggt
     1921 cagttatgga atcgagatcg tcagttatgg aaagcaggga ctggtttata aaggttcaga
     1981 acgatctatc cgtacgatga tctatgattt tatcaatcag aatctgggga tgaccgactt
     2041 ctttatgaat caagatgcac aactaacacc ggctcaaaaa atctttttcc attattttcc
     2101 tttggcggaa atcaaaaaaa tagaggagat ctattccaat aagttgttaa aacaagagga
     2161 tgatatttat aaaaagcaaa ttctgctttt tacattgatc tggatccaac gaatcaggaa
     2221 acatgaattc atctctgcca ttaattggaa aaatacagaa ataaaagaca gcagcttctc
     2281 tgcatttatt gactacatga tcacggaatt tcaattggac aatgttccac aagtagaaag
     2341 aaattatctt cgtttcacta tcgagacgtt caatgctaga gatatctcca attcgattga
     2401 gtgggtacaa gctcaactac taacgatcca attgatccaa tttgtagaga acgagacaca
     2461 catacctttt catctaaaag aagaagcttt attcgaaaat ctctataaac acatggctgc
     2521 actgatcata agaatcaagc atgctattca agtcatgaat ccattgaaag aaaacatccg
     2581 tacaaattat tttccaatcc accgcgcagt cactcatttt gttcctacga tcgaagaagt
     2641 aatcggcggc aagatactgg aggacgagat agcttttctt gtcatccact tttcgacgat
     2701 tgccagtgtg atcaagcgag acctgaatta tgtttatcaa tcagttgttg tctgtaatca
     2761 cggcatggct accgggaatc tgttagcaga aaatctgaaa gaaaaatttc ctcaaatcga
     2821 gattactgca gttttaagtt caaaagaaat cgaattagtg gataaactgg atgtcgatct
     2881 ggttttcagc acctttcaat tgaattatcg aaataagccg cttttagtta tcgatccaat
     2941 tttgactgag acgaatattt tagttataga agactttttg aaaagaaatg aagaactgca
     3001 gcggttaaaa cctaacagtc atgaatcaac gcagttattc actcaaataa tcggtgtcat
     3061 ggaagaaagt ggtggccgag tagatcgcat catctatggc aagttggaaa aattatttga
     3121 aataaacaat ctagaaatca acaaaaggga gatccagcct atgttaaaag atattttgac
     3181 ggacagcaat atcatgatca aagaaaaagc cgctacatgg gaggaaacga ttgaacgagc
     3241 tgctaaacca ttggtcaaag aaaacattgt tgaagaacgc tatgtcgaag cgatgattcg
     3301 cgcagtgaat gaatacgggc catatatcgt gattggcaaa cacatggcgc tagcccatgc
     3361 tcgaccagaa gatggggtca ataagttagg agtcagcgta gctacgattg agcaaccaat
     3421 cgatttcggt aatcctgaaa tggatccagt aaagattatt ttttgtttag cggcagtaga
     3481 ttcctactcg catctgaaca tcatgaaaga actgatcgaa ctgatcaaca atgaagagaa
     3541 gttggatcga ttgatcgagt gcacaaatgt tgaagatttc aaacaactgc tttttcagta
     3601 gttgagaaat aataaatgat acgggggaga acaaagatga acaaattaaa cattttattc
     3661 gtatgcggcg caggtttagg aagtagtttt gcagcacaga tgtctgctga ggatgttttg
     3721 aacaaattag gagtagaagc caaactggac cattgtgata tttcttctgc agtgtccatg
     3781 aaaccagatg tgatcatcac agcagaaaat tttcgctcgc agttcgaaaa attctcagtc
     3841 gatcctaaaa cagcgatgat ctatttgaaa aatattgtct cgaaaaatga aattgaagaa
     3901 aaaatcaccc cagttctaca agataaggga gttatctaaa taaaaaagaa aagaggataa
     3961 aaacatgggc gttatcaatt ttatcatcga aaatattttg actcaggcat cgatcaccat
     4021 tgcattgatt gctatgttgg gtctactgct gcagaagaaa tcagcaggac aagtcatttc
     4081 tggcacgtta aagacactcc taggattcca agtgttgagt gccggttcga gtatcatcgt
     4141 tggaagttta acttatttcg ggaaaatttt tactgaagga tttcatatgc aaggaatcat
     4201 tccttctatt gagtctatca atggtcaagc aatgaatgat ttaggtttag gtagagatat
     4261 tgcactgacg ttcttagcga tttttgtatt caatattatt ttggctcgtt tcacaaaatg
     4321 gaagtacatt tttctaactg gtcaagccat tttatggatg gcgacgatga cgacggtctt
     4381 tggttatttc gctggtctgc gagggatcgt attgatctta gtcggtggtt ttatcggagc
     4441 ttgtttcgcc attgctatgc cagcagtagc gcaaccaatt atacgtaaga ttacaggatc
     4501 gaatgatatt gctttagggc atttttgtac gattggatac ttatttgaag ctggcgtggc
     4561 taaacttttt ggtgagaaag gtgaaaataa aaaatcgatt gaagagatca aattgcccac
     4621 gcatttcgag tttctgcaag atacgtattt gtctgtcatg gtcgtgatgg tcccactata
     4681 tatcatcact gttttgtttg caggtgaacc attcgcttct gaattatcag gagatcaaaa
     4741 ctatatcatg tttgcctttt tacaagccat ccaattcgta gtgggtgtgt atgtattgct
     4801 agctggcgta cgtctactgt taggtgaaat cgtgccggca tttcgaggga tagctatgaa
     4861 attagttcct gatgcgattc ctgccttaga ttgtccagtt ttctttccct atagcccaaa
     4921 tgctgttatt ttaggtttca ttacgacaac aatcggtacg attattgcca tgttcacttt
     4981 gccgatgttc ggcttagcga tgattttacc aggtatgcta acgaatttct ttgccggagg
     5041 aacagcgggg atatttggta acgcagttgg cggacgtcga ggagcgatca ttggcggtat
     5101 tgctcatggc ttctttatta cattattgcc tgctttgcta gtaacgatct ttaattcgat
     5161 gggattcatc aatgctacag caaccgatgt agatacagta gccgccgcac tcttatacgc
     5221 ttggatctta agcccaattt tgaaagcatt ttaattgaaa gtagggaaaa tgatgttcgg
     5281 attttttaag aaaaaagaaa atgaaccaaa aaaaagatcg ataaaagagg aaaaaatcct
     5341 ctcgccagaa gtgaccgaaa agattcaatc agagattaca caattaaacc aagagatttc
     5401 aacgacccaa gagaagccca aattagcgga actttacgaa caagttggac ttaaattttc
     5461 tgaattgtat gagaacgatc aagccatcca atatttagaa aagagcttgg aaaataaaca
     5521 gacgatcgga gatgggtata aaaaactgat gagcctctat aatcagaagc gtgcagatgc
     5581 tgctcgtgcg ggtgatgatc aagggatcga ttactacatg ggcaaaatgg atgtgatgcg
     5641 acaaattgca aaacaagtaa cgatcaaagg aaacaaataa aaagataagg tggcaataaa
     5701 aaatgtatac aacattgaaa gaagtaacaa aaacagcaga agagttgaat tttacagttg
     5761 gagcttttaa tacgcataac ctagaaatgc ttcctgccat gctaggcgca gcaaaagaaa
     5821 tgggttcgcc aatcattatt caaacaagtg tcgacaccgc aaaatatatc ggtatgcatg
     5881 tgttagtcaa tgtaatgaca gctattgcag aagaagaaat ggttgatgca gtcttgcact
     5941 tagaccacgc ccgagacttt gatgatatca aagaagcaat cgatagcgga tatacttctg
     6001 tcatgtatga cgggtcccat ctgcctttca aagaaaatat cttgaagacg aaagcagtgg
     6061 tagaatatgc ccatgcacac ggtgtttccg tagaaggaga acttgggacg atcggcggta
     6121 cagaagaagg aatccatgtc gctgaaaatg acaaagtata tacaagaccg gaagacgcag
     6181 tgaaatttgt agaagctaca ggtgtggatg cattagctat tgctatcggt acgaatcatg
     6241 gacaattcaa atcaaaaaca gaagtgaaca ttcccttgct gaaagaaatc gatgcagtag
     6301 tcgatgtccc cctcgttatc catggcggaa caggtgtgaa agaagaagat tatcccgaat
     6361 tgatcaataa cgggatccgc aaattcaatg ttggaacgga gcttttagtc aactggacaa
     6421 aagtggctaa aggaaccttt agtgaaacag aaatcaacaa gtctcttcgc cataatgtga
     6481 ttccagcaaa tgaagcagta aaagaaattg tcaagcataa gattggactg tttatgaact
     6541 tggaaagtcc aattaactta ggaaacaaat gagatgaaaa agtatttcat tcttctagct
     6601 ggtagtcctg gtacaggaaa aacatatctt atgaataaac tacgggaacg atttccagat
     6661 atgtatgcca tcacgctcga tgaaatcaag gaatattatg cagaatccgt cggttttaac
     6721 aacttggaag aacgggcgaa acaagaaagg acgaaagttt atcctttcta ttacaaagca
     6781 cttgatctgt atatggaagc aggaaagaaa gtgattgtgt cagagtatcc ttttagtgat
     6841 aagcaaaaaa ctcgactcca agaattagca gaaagatacg gatatcaggt aatgacgatt
     6901 cgcctaacag cagactttga agtgttatgg caacgtcggt acaaacgtga tcgtgaacca
     6961 gatcgtcatt tgagctatat catggatcat tatcactatg gcgacatatt ggaagatcga
     7021 tcgaaaggga cgaatcatat tacgaaagaa gcattcaaaa aaatcatcac tgaccgaaaa
     7081 tatgacgaat ttcagttagg aacactatat gaatttgatg taacagacta tactcaagta
     7141 gattacactc cactattgga taaattagaa aaagaaatca atgacaataa gtaacgaaga
     7201 aatggtttga tagtgacagg taataaaaaa gacatttggt ccccagagga gccaaatgtc
     7261 tttttgttct aaaatgtttt ttcatattct tttatttatt gccgatttcc ttgtgtttat
     7321 ttgccgtaat taggtcagtc ttctatactt gattacatag tcatacattt gctgaaaaga
     7381 agggaagaga atgaataaca agttggccca atacaaaaga ctgctcgcac tttctcaagc
     7441 tggtctgtat tatggaaaag atgtattcga taaagaacga tttgaagaga ttcggacgat
     7501 ctcattggaa ttgatcagtg aaataggaaa agaaactttc gaagaaataa aagcacttac
     7561 agttattgaa gaaggtcatc ccacaccaaa agtcgatgtt cgtgcctata tcagaaagga
     7621 cggcaagatc ctactaatcg aagataaacg gacgagagaa tggtcactgc caggcggatt
     7681 tgccgaaata ggtttgtccc ctgaagaaaa cgtgcggaaa gaagtctatg aagaaacggg
     7741 tttaacagta gaatctactc agctgcaagc ggtttttgat acgaataaac aaaaagatat
     7801 tccgcagctt tttcagtatt ataagcttgt ttttgcttgt acgatccacg acgaagatgc
     7861 aaaatttatt gagaacaatg aaacttccga catgggattt tttagtatag aagagctgcc
     7921 aaagctatca gaaaaacgaa caacaaagaa gcagttgctt attttagaaa acaaaaatca
     7981 gctctattgc gattgagttt tttcgtaaaa gaagaaagct gtttactctg cgagttctct
     8041 aaaaaagaac tcgttctttt attaaaaaat agttgcattt gcaacaaaaa gaggtaaact
     8101 agtcttacaa acaaaataaa ggtggggtga aatggctgaa attcttcgag acattggaat
     8161 gattgcccga gcgttggatt ctatcagcaa tatcgaattc aaagaagttg atttaacaag
     8221 agggcagtat ctttatttag tacgtatttg cgaaaatcca ggaatcatcc aagaaaaact
     8281 agcagaaatg atcaaagtag atcgtacaac gactgcacga gcaatcaaga aattagaatc
     8341 caatgggatg atcgaacgtt tggaagataa agaaaataaa aaaatcaaga aactttatcc
     8401 gactaaaaaa ggagcagaaa tctatccttt catcatccga gaaaacaact actctaatgc
     8461 agtcgcatta aatggtctgt ctgacgagga agcaaaacaa ttggaatatc ttttaaagaa
     8521 agtatgcaag aatgttagcg aagattggaa ttttgtcaaa aaaggtaata aaaggatcta
     8581 ttgaaaggaa gaagccgtga ataaatacga aattcgagaa atgacgcctt cagatgtaga
     8641 cttactacaa caaatcagta tcgaaacatt tacagataca tttggaaaag ataattcgcc
     8701 ggagcattta gctgcttact tgagacaagc ttatgcgcca gaaaaactta cagaagagct
     8761 aaaagagaaa gaaactgatt tttactagta agtatggacc aagagcctgc gggttacctg
     8821 aaactaaata taggaacgag tcaatctgag gaaatggatg atcatttttt agaaatcgag
     8881 cgaatctatg tacgaaaaaa atttaaacat caaggtattg gtacattttt actagataca
     8941 gctatcaaaa aagcaaaaga aaagcaaaaa acagccattt ggctcggtgt gtgggaacac
     9001 aacgatgctg cgctcgcttt ctatcgcaaa agaggattta ccaagtacag tgaacatgtt
     9061 ttttatatgg gagatgatcc ccaaacagac tatctattca tcaagcaatt attctaatca
     9121 agaggtgtga acatatgaaa aaagtaatag tggatgccaa tttttgggaa gtatttcctg
     9181 aagcaaccat cgaaatctta tccgtgagtg gtatcgataa tcacgtaaca gaagaaaatg
     9241 aagaaatgta tcatcaatta ttgaatagcg cggcaaaaga agcaagaaat taccttacgg
     9301 aagaaacatt cagccaaaac gaagtcattg ctcagtggcg tcaggctttt accacattca
     9361 agactaaaaa aggtgcacgt tcttctattg aagctttact gaaacgtgca aatcaagaac
     9421 gggaattttt cccaatcaat ccgcttgtag atatttataa tagtatttcc ttgtcttttg
     9481 gcgtaccttg cggcggagaa gatatcgaaa agatttcagg aaatctgcat ctaggaaaag
     9541 cccaaggtgg cgaatcattc ctgcctttag gtgcagattc cgatgctccg gcacttccgg
     9601 aagaaatcat ctactatgat gaacaaggag caatctgtcg ctgtctcaac tggcgtgaag
     9661 cccaaagaac gatgctgacg gaagaaacgc aagcagcggt gctggtcatt gaatccatca
     9721 atgctgaaca agcggcacgc ggcgcacagg caatcgaagc gttgaaggaa aaaatcgatc
     9781 aaacatttgg cgtgtccatt gtcagacaca cactaaccaa agatgaatca gaagcagaat
     9841 tataaaagaa tcgaaaaaag aagagcggca ttcattgccg ttcttttttt gtaaaaaaag
     9901 tttgtggagg aaagcaagtc tagaaactat tgctttattt ttcttgaata ggatcaaaca
     9961 caaggtgttc gcgctttcgt gatataatgg aatcagttta tagacaggaa agaaaatcaa
    10021 ctagaaagaa gggtcaaacg atgatacaaa aaacacgtgt gatgctttat gtcaatgacg
    10081 tagagatgat ttgccgattc tttgtagaaa aaattggtgc agaaatttca gaaacgatcg
    10141 aattgccaga agaatttaag agtattgtgc tgagtatctc aaaagaatta gaattaggta
    10201 tatttccaaa agtatttgtg cagaagtttt caccagaagt attgggaccg ccaccttctt
    10261 tagtcttttt tacggatgaa tttgaaacta tttacgagaa catggacgaa cctggagaaa
    10321 tcacagacaa caatggcgtc ttgactttca atttttcgga ccctgaaggc aattattttg
    10381 ttataggaaa agctgagtcg tctgattgaa taaaaaggag gaaaaacagt ggatctaatg
    10441 ggaaggctag aaggttattt gaagtatcgg cgagcaaaac atagtgagaa gcttcgagca
    10501 aggaatctca aatcagggaa aaagaagaca aaaggctggg gaaaaatggt cgatacaggt
    10561 tttggcggac taaataacct aaatcctgtc gatacaaaag aattcatcaa agaagagcag
    10621 aaacgaaatg gtaaagtcaa atcacactat tgattttgat actagaatga agaaactttt
    10681 ttaacacaat aaagaaattc ttttgatcca aagtgaatgt tcatggactt tgagataaag
    10741 ggatttgaac agatggaacg ttcggatttt gcttaaaatc cgaatttttt gtttacttat
    10801 gttttgtttt agtatatgat aaaacacgtt gttttattaa tcaatcgaaa ttttaacgga
    10861 ctttatttat aatgttcgga tttaggggga tggaagatga aggaaaaaat actttcctac
    10921 ttcgaaatag aaaaattgaa taccacggta aaacgtgagg tactggcagg atttacgaca
    10981 tttatttcaa tggcctatat cttatttgta aatccaactg tattaggcgc ttctggaatg
    11041 gatgaaggag cggtgttcac tgcgactgca ttagccagtg caatcggctg tattttgatg
    11101 ggggtattag cccgttatcc aattggaacg gcacctgctc ttgggatcaa tgctttcttt
    11161 gcttattctg tttgtttagg gatggggatc ccatgggaaa cagcattgtc cggcgtattc
    11221 gttgcttctt tgatctttat tttgattaca atcttcaaac tgcgggaaat gatcattgat
    11281 gcgatcccag cagatttgaa atatgcgatt tctggtggta tcggtctatt tattgctttc
    11341 cttggtctga gcgaaggtgg gatcatcgtt tcgaatgaat ctacattagt aggattagga
    11401 tcactcaacg ttggttctac ttggttgacg atttttggat tagtcgtcac ggctatgttg
    11461 cttgttcgac gtgtaccagg tggcattttc atcgggatgg cagctacaac gattttaggt
    11521 ctggtcacag gtttgattcc aatgccttct gaaattgttg ctgcagcacc aagtttgaaa
    11581 ccaacatttt tagttgcgtt aaaacatgta ggggacatca attctcttca aatgtgggtc
    11641 gtagtactaa cattcttgct tgtgactttc tttgatacag caggaacttt agttggatta
    11701 gcaaatcaag caggctttat gaaagacaac aagatgcctc gtgtagggaa agctttagct
    11761 tctgattcaa cagctatgct tgcgggctcc ttattaggta cttctcctgt tggtgcattc
    11821 gtagaatcat cagctgggat tgctgttgga ggacgctcag gaatcacagc tattactaca
    11881 gggattttct tcttgttagg tttgttcttt tctccattac tgtcagttgt tacttcacaa
    11941 gtaactgcac cagcattgat catggtaggt gttttgatgg ctcaatcatt acgtcaaatc
    12001 gaatggggaa aattagaaat tgcgatccct tccttcttga tcttgattgg catgccttta
    12061 acttatagta tttcagatgg gattgcatta ggttttattt tctatccaat tacgatgtta
    12121 gcggctaaaa gaggaaaaga agtatctcca atcatgtatg gtctgttctt tgtattcgtg
    12181 ggattcatgt ggatattgaa tgtgaaataa agaggttgtg aaatcagcgt tctggcctga
    12241 gtattagaga aaaaaacgaa aaaatagttc ctcctatttt gccgtttttg gagctaatac
    12301 cgagggacag ctgagtgaac accgtttata aagaggttgt gaaatcagcg ttcttccctg
    12361 catcaatcaa tcctttcaaa aaacagttga ggaaatttcc tcagctgttt ttttgtttta
    12421 ttctgttacc gataacaagg tgcaatgact atttgaaagc gttacaattt ttggtagaag
    12481 aaactaaact agatgattaa gcgaaaaata gaaacatcca tttataggag gaaaatcatg
    12541 gaacgaaaat ggtggcacca ttcagttgtt tatcaaatct atccaagaag ttttcaagat
    12601 tcaaatcaag atgggattgg agacttgcga gggattattt ccagattgga ttatctggca
    12661 tatttgggca ttgatgtgat ttggctttcg ccggtgtacg agtctccaaa tgatgacaat
    12721 ggctatgata tcagcgatta tcaagcaatc atggcagaat tcggtacgat ggaagaaatg
    12781 gaggaactga ttgaagaagc aaagaaaaga aacatccgta tcgttatgga tctggtggtg
    12841 aatcatactt ctgatgaaca cccgtggttc attgaggcac gtaaaaataa agaaaatcct
    12901 tatcgtgact actatatttg gcgtgatcca gtcaatggag aggagcctaa tggattgcgc
    12961 tcgatattca gtggacctgc ttggcagttg gatgagcaaa cagggcaata ttttctgcac
    13021 ttgtatagca agcgtcaacc tgatttgaat tggaaaaaca aacaggttcg ccaagcgatt
    13081 tatcaaatga tgaatttctg gatcgacaaa ggtattggtg gctttcgtat ggacgtgatc
    13141 gatatgattg gcaaagaacc ggataaagaa atcacaagta atggacctaa actgcatgag
    13201 tatcttcagg aaatgaatca agcaactttt ggcgacaaaa atctgatgac cgttggcgaa
    13261 acatggggag caactaccga gattgctaaa ctttattccg atcctaaaag aaaggaatta
    13321 tccatgatct tccaattcga acatatagga ttagatcagc aagaaggcaa agacaaatgg
    13381 gatttgaaac ctttaagtat ctctgaactg aaaaaagtat tatcgaaatg gcaaacttct
    13441 ttgggtgatg aaggatggaa tagtttattt tggaataatc atgatctgcc tcgaatcgtc
    13501 tcacgttggg gaaatgacga tttgtatcga aaagaaagtg ccaagatgtt tgctatcttg
    13561 cttcatatga tgaaaggcac tccttatatt tatcaaggag aagaattagg catgacgaat
    13621 tgcccaattg atgatatctc ggaagcaaaa gatattgaga caatcaatat gtataacgaa
    13681 aggatttctt ctggatttac aaaagaggaa atcttggaat caatcaatgc gaaaggaaga
    13741 gacaatgcta ggacgcctat gcaatggaac gcccgtgaac atgctgggtt tactactgga
    13801 attccatggc ttcgagtcaa cccaaattat aaagaaatca atgcagaaga agcgttagca
    13861 gatcaagatt ctgtttttta tatgtacaag aaattgatcg atttgcgtag aaaacatgac
    13921 atcatcgtct ggggagacta tgaattagta gaagagacgc cagagcatgt ctttgcctat
    13981 tctcgcatat atgaagacca gcgatggctc gttgtttgca atatgtccag tgaaaaacag
    14041 gtgttcactg ttccacgtga agctttaagg attattgtgt caaattatcc tctgaaacag
    14101 ttaccagacg gcaaagtcga gttacgacca tatgaagcat ttgtgatgga agtaaataaa
    14161 gaaaagctta tttgattgaa acgtattacc gagaggttgt gacacatttt ttgtcacaac
    14221 ttctttttct caaaactaaa cagaggaggc ggtcattact ccgtgttttt tgacaaaaaa
    14281 taaaaatcat actatgatat gtacgccaat ggaggggatg aaatggaaaa gccaattgaa
    14341 aaagcgttgg atgaatgtct tttctttagc gtgaaaaagc tagatcgcct attgaataaa
    14401 ttagcggatg aagcgtttag aaaaacaggt ctggcaccaa cttatggatt tattttattg
    14461 atattaaaag aaaaagatgg tataccgcaa aaagatattg cacaaatgct ctattctgcg
    14521 ccttcaacaa tcgctcgttt tgttgaaaaa ttagaatata aaggatacgt caaaacagtt
    14581 agcgaagggc gcttgtcatt agtttatctt acagaacaag gacgccaatt tactaaggaa
    14641 gtcgatggta gctgggaaga actccatcag tcgtacaaag cagtcttggg aaatgaagca
    14701 agtaaagaat tagccgagat aatgaatgaa gcaacagata agttacaaaa aaaataaaaa
    14761 tttttttaag caaattagtt gcatgtgcaa ctaaccgagt ggtttaatga taaatgattc
    14821 tcgataggtt aatcagagcg aattatggga ggaatgacaa tgtcacaatt tgatcaatca
    14881 attattttac cttcaggagt tcaattgaag aaccgattga tgctgtcgcc tatgacgaca
    14941 caactcagct tcttcaatgg agtgatcacc gaagatgaga tcacttatta taaacaacgt
    15001 tctaaaggac ttggtgctgt tattactggt gctgcaaatg tccaagatat cggaaaaggc
    15061 tggccaggcg aactgagcat cgctcacgat gaaatgattc ctcggttaag tgaactagct
    15121 aaagcaatcc aaggagaagg tgcaaaagcg attgttcaga ttttccacgg tggacgaatg
    15181 actagtcgag ctactctatc cggagaacag cctgtatctg caagtgcagt tgcagcggaa
    15241 cgacctgatg cagaaacacc gcgagcatta agtgaaaatg aaatcaaaga aacaatccaa
    15301 gcatttggcg aagccacacg cagagcgatt gaatcaggat ttgatggtat cgagttgcat
    15361 ggatcaaata cgtatttgat ccaacaattt ttctctccgc attcgaatag acgagaggat
    15421 caatggggcg gtagcttaga aaatcgctat cgttttattg acgaattatt aacaacggtt
    15481 ttctcaacgg ttgaaaaata tgcaaaaaaa ccattcatcg tagggtatcg tttctcgcca
    15541 gaagaatatg aaacaccagg tatccgtttt gaagacacga tctggcttct tgaacgatta
    15601 cgtgagacaa aattagatta tctgcatgtt tcattaaatg cgtatgatcg aagagcccgt
    15661 tctgataaat acagcgatca gtcgattctt gaagctgtcc atgacacgat ccaaggaaag
    15721 atccctttaa ttggtgtcgg aggcatccgt actcgcaaag acgtagagca agtactgaaa
    15781 tatgcagaac ttgcagcaat tggacagcaa atgatcgtcg atcctgactg ggcagagaaa
    15841 ttattggaaa accgtgagga tgaatttgta acgaaacctt ttgaagaagc atataaagag
    15901 ctttatttgc caagtccatt atataatttc ttgaaagcac gttatcaata actcaatttt
    15961 tatagaaaga ggatggaaaa atgaaagtat tgatcgtatt aacaaatgta gaaaagtacg
    16021 aaaagatcga gcgtcctaca ggtttgtggt taagtgaact gactcatttt tatgatgtat
    16081 tagtaaaaaa tgggatcgat gcagattttg tcagtcctaa aggcggatat gtacctttag
    16141 aaccgcaaag tgttttgaat atggatgaaa tcgattgggc ctattataaa gatgaagatt
    16201 tcagaaatcg ggccttggga aataccttac gtcctgatga tgtggatgca aaagattatg
    16261 cagcagttta ttatgctggt ggccacggaa caatgtggga ttttccagaa gcaacagatc
    16321 ttgggaaagt tgcttcacaa atctatcgaa atgaaggact ggtctcagct gtttgtcacg
    16381 gtgttgtcgg attgttgcca gttacagatg aatctggaaa gtctatttta gctggacgca
    16441 cagtgactgg attcagtaat gaagaagagg aagccaatgg tacaacgaat gaagtaccat
    16501 tcttagcaga agatgctttg aaagaaaaag gagctgacta tcagtcaggt ccagcttttt
    16561 cagaagtcgt tcaaatcgat ggacgtcttc ttacagggca aaaccctcaa tctgctcatc
    16621 aattgggcga agtagtggtt gaagcattaa agcattaaaa attgtcagca taaaaatgtt
    16681 ttgcctattc taactattac attacacacg ctaaaacacg tcttttcgtt cttttctgtt
    16741 tcctatttgg acagaggtac gcaaaaggcg ggttttttgc ctttctaaga gtacattttt
    16801 tgaaacatct cttcagaaat gatactcttc aattaggtca actaatttat tactgagaag
    16861 cagacctagt cacacggtag gatggacaga gtgttttctg ctacgctgct atggatcata
    16921 ggaatgagta aacatggttt aagaacattg tgatttcagc ccatgttggc agtcgcacac
    16981 ttaataagaa agaggaatac agatgaacat tgtaattgtc ggcgggtcgt ttgcagggat
    17041 tcatgcggct atttatcttc gccaattgat accaacaagt gaaatatatt tgattgaaaa
    17101 gcaatcgaag ttagggtgga ttcccagcgg gttcaatctg atagtaaaag gaaaggtcac
    17161 cagtgaaaaa caactttctt ggatcaccaa agaagagctg atcgaccgct atcgtatcca
    17221 tgtttacaca gaaaagacgg taataggatt ggaagaaaag aaagtcgttt tagcaggcgg
    17281 agacaaactg gattttgatc gtttgatttt ggcgactggt tcaaatcaaa attttcgaaa
    17341 tgttcctgca gaagcaaggt tcattcaccc agttaaaaag atacaaaaca tagctgctct
    17401 tcagcaaaag atcatggatg caaaaaaaat tgccatcatt ggagcaggtc aagcagggat
    17461 cgaaatagct gaaggcttag catcaggaaa aacacaaatc catctttatg aaagccgaaa
    17521 gtcgattctt tttcgttacc tagaccctga aatgacggag ccgttagtaa aggaaatgaa
    17581 aaatcaaggg atatcgcttt tcttagaaga gcaagtcgta tctttgtccg aaaatgaaac
    17641 cgctgtagta gagacggaaa gaagaaggga aaactatgat cttgttttgc tggctaacca
    17701 ttctcgcccc aacaatcaga tgtgggaaga acaattaaca ttgaatgatg atggaacgat
    17761 ctgggtagat gactatttgc aaacgtctca aaaagacgtc tatgcaattg gcgatgcaat
    17821 ccaagtcact tttcgcccaa caaatgaaaa aatgtacgtt tctctagtaa acaacgctgt
    17881 ccgtacagcg cgaaatgtca gcaagacaat tagtggttct gcgacaaagg atcagggaac
    17941 ctatcgacca gtcggcaatc agtggtttgg ctatttctta ggaagcgtag gtatgacaga
    18001 agaagaaagc attttttatc cccaaaaaat tgaaacaggg tacgcggaat acaagcttgc
    18061 agcaataaac cagagcaaag tcaaagtcaa attcctattt gattctgaaa aaaagattct
    18121 tggagcacag ctccaaagca gaaaagaaat gttccatctt cttgaccaac tgacaatcgc
    18181 agtagaagaa ggctggacgt tcgaacagtt agaagaacat gagctgtttt tccagccaga
    18241 atatcgtgta cctaatatgg tgtggtcaaa ggtggcgaat actgcctatg aagattgaag
    18301 cattattaga aagaaaagag cagttacaga tcctgatttt gcgaaacctt gttctgcaag
    18361 ggggaacagc ctctatcaat ggattgagag agcatgtcca actgtcaaaa acctcttttg
    18421 atcaatattt ggaagatatt gaattgattg gacggatgat gggaaaaaaa gtagaagtac
    18481 agcgaaacga gtatcaggcg ctgcttgtgt tggacgaaga tgtcagtttg gaaaaaattc
    18541 ttttgttttt attacaggaa agcttgaagt ttaaaatgct tgtctattta ttagaacacc
    18601 agcaagtatc catcgttcga ttagcgacag cgtttaatat cagtgagtct tctgtttttc
    18661 gaaagatcaa agaattgaac cagctcttag aggaatttgg attacaaatc aaaaacggac
    18721 aattatacgg agaagagcta cagatccgat atttttatta tgaattattc caatatatcc
    18781 cggaagatca acgaccgctt tttttgcaaa atacaccaga aaaaagacca ttcattctag
    18841 gattgggccg cgtattagaa acgacgttta cggcatcagc agaagcacag attgcttgct
    18901 ggctcgggat cacgaaaaaa agactattga atgaaaaaag tacgtacgaa accttgaaag
    18961 aaaagaaatt actctatcag tcggaccggc tttatcaagc aatcgatcca atcatcgtga
    19021 tgcatcttag tcgaacagca gcagaactga atgtatacga aacaatgatg ttttacagct
    19081 tttttgtttc tttttcgata gtcgacgaag aaatatttta tcaatatgat ttgacacgga
    19141 gcaaaaagtt acccaccgct gttttggata cttatattcg ggaaacgatg ctatggcatt
    19201 atcgtcctcg tcgtttgaaa atcaaagagg aaaaagcagt aggctaccaa atttcacaaa
    19261 tcaacaatga atggtttttc tttgttggaa agatcgaagt ctacgagcgt gatcggcttt
    19321 tggagcagca gcaaaaaatg cttgggcatt ccttaacaca gttattggag aaactacagg
    19381 aaacagcgat gcagcaattg ccaaggaaaa gcaccgaaga cagtgcactt agttatctga
    19441 tgatccagta tgccaatgtt cttttgatga tcgattttta tattgcgaaa ccagtagtga
    19501 tagggatcga tttagaaagt ttgcctattt accgtattgc tttccaacag tatctgaaca
    19561 gagaactgag aggagtcagc ggtattgaga ttgaaagtta tgaagaagga aaaaactatg
    19621 atttggtcat aacgttttgt cagagaaaca agcaacagaa tgaatattat ctatcagaat
    19681 ttgcgtcgcc ttatgacatt atccgattga aaagaagaat agaaatgttg aaaaaagaaa
    19741 agaattgatc aaaaactagc atcataataa aaaactgtca aaaaaaagaa aaaaagaata
    19801 aaattttgac agtttctttt tttaaatgag tagtatgata aaagcagtta aagaaagcgt
    19861 ttgcagataa tggagggaat cacaatgaca gaaccaacgt atatcatggc gattgaccaa
    19921 ggtacaacta gctcacgagc aattatcttt gacaagaaag gaagacatat cggcagttct
    19981 caaaaagagt tcacgcaata tttcccgcaa gaaagctggg ttgaacatga tgcaaatgaa
    20041 atctggaatt ctgtacagtc agttattgcc ggagctttca tcgaatcagg gatcaaacca
    20101 aaccagattg ctgggattgg cataacgaac cagcgtgaaa caacagtaat ctgggaaaaa
    20161 gatacaggac gtccaatcta tcatgcagtt gtttggcaat ccagacaatc agctggaatt
    20221 gcagataact taaaaaatga aggatatcaa gaatttttcc acgaaaaaac aggactagtg
    20281 atcgatgcct atttctcagc aacgaagatt cgctggattc ttgatcatgt agaaggtgcg
    20341 caagaacgcg ctgaaaaagg cgagttaatg tttggaacga ttgattcgtg gttagtatgg
    20401 aaactgaccg atggacaagc acatgttact gattactcga atgccagtcg gacgatgttg
    20461 ttcaatatcc atcagctaga ttgggatcag gatattttgg atttattgaa tattcctcga
    20521 gtgatgttac caaaagttac ttctaactca gaagtttatg gttatacgca aggttatcat
    20581 ttttatggaa gcgaagcacc aatttcaggg atggcaggag accagcaagc tgcattattc
    20641 ggacaaatgg cattcgaacc aggtatggtc aagaatactt atggtacagg ttcatttatt
    20701 gtcatgaata ccggtgaaaa accgcaatta tccaaaaata atttattgac gacaattggt
    20761 tacggtataa atggaaaggt ttactatgct ttagaaggaa gtatttttgt ggctggttct
    20821 tcgattcaat ggctgagaga tggtcttcaa atgttgcaaa aggctagtga ttcggaagcc
    20881 gcggcaaaac gctccacaaa cgaagatgag gtctatgttg ttcctgcatt tgttggactg
    20941 ggtgctcctt attgggatca ggcagcacga ggggctatgt ttggtttgac acgtggaacg
    21001 acaaaagaag atatcattaa agctactttg cagtcgattg cctatcaagt gagagatatc
    21061 atcgatacga tgcaagatga cactggaatc aagattcctg tattgaaagt ggatggcggt
    21121 gcagcaaata atgaatatct gatgcaattc caaacagata tcttgaatgt accgatccaa
    21181 cgagcagaaa acttggaaac aacagcacta ggggcagcat ttttagcagg attagctgtc
    21241 ggctattgga aagatacaga cgagattcgt gaattttatg aagctggcaa gctatttgaa
    21301 gttcaaatgg aggaagagcg ccgagaaaaa ctttacaatg gctggaaaaa agcagtaaaa
    21361 gctacccaag catttgaata aggaggaaat ccatatgttt tcaaacaaga caagacaaga
    21421 tagcattcaa aaaatgcagc aagaagaatt ggatctgttg atcatcggtg gcggaatcac
    21481 tggtgccggt gtggcagtcc aggcagcagc atcaggaatc aaaacaggat tgatcgaaat
    21541 gcaagatttt gcagaaggga cgtcctctcg ctcgaccaaa cttgtgcatg gcggtattcg
    21601 ttatctgaaa acatttgatg tggaagtagt agctgacaca gttggtgaac gtgcagtcgt
    21661 acaaggtatt gccccacaca tcccaaaacc agatccaatg cttttaccaa tctatgaaga
    21721 tgaaggagca acaaccttca atatgttctc tgtcaaagta gcaatggacc tttacgacaa
    21781 actggcaaat gtgacaggaa ctaaatatga gaactatacc ctgacaccag aagaagtatt
    21841 ggaaagagaa ccatttttga aaaaagaagg gctaaaaggt gcaggtgttt atctggattt
    21901 ccgcaacaat gatgcccgtt tagtgatcga taatatcaaa aaggctgcag aagatggggc
    21961 ttatctagta agtaaaatga aagcggttgg ctttttatat gagggcgatc aaatcgttgg
    22021 tgtcaaagct cgtgatctgc tgacagatga agtgatagag atcaaagcaa aattagtgat
    22081 caatacgagt ggtccttggg tagataaagt acggaacttg aattttacgc gtccagtctc
    22141 tcctaaaatg cgtccaacca aaggaatcca tttagtcgta gatgcgaaaa aactgcctgt
    22201 accgcaaccc acatacttcg atacaggaaa acaagatggg cggatggttt ttgctatccc
    22261 aagagaaaac aaaacttact ttggtacgac agatacggat taccaaggag actttacgga
    22321 tccaaaagtc acacaagaag acgtggatta tctattggat gtaatcaacc atcgctatcc
    22381 agaagcaaat atcacattgg cagatatcga agcaagctgg gcagggcttc gtccactatt
    22441 gattggtaat tctggttctg attataatgg cggagataac ggatcgattt cagacaagag
    22501 cttcaataaa gtggttgata cagtaagtga atataaggaa aataaagttt ctcgtgctga
    22561 agtagaagat gtgttgaacc atttggaaaa cagccgcgac gaaaaagcac cttctacgat
    22621 ttccagaggt agttctttag aaagagaacc agatggcttg ttgactttat caggtgggaa
    22681 aatcactgat taccgtaaga tggcagaagg agctttacga ttgattcgtc agctgttaaa
    22741 agaagaatac ggaatagaga cgaaagaaat cgactctaaa aaatatcaga tttcaggtgg
    22801 aaacttcgat ccaacgaaat tagaagaaac agtgagggaa ttagcaaaag aaggagtagc
    22861 agccggttta gaggaagaag atgctactta tatcgctgat ttttacggga ctaatgctcg
    22921 acgtatcttt gaattagcaa aagaaatgac accttatcct ggtttgagtc tcgctgagtc
    22981 agctcggtta cgttatggct tagaagaaga aatggtttta gccccaggtg attatctcat
    23041 tcgtcggacg aatcatctgt tgtttgaacg agaccagctg gatgagatca agcaacctgt
    23101 gatcgatgca attgctgagt attttgggtg gacagaagaa gagaaggtgc aacagactaa
    23161 acgtttagaa gcattgatcg cagaatcaga tctgcgggaa ctaaaggggg agaaataaat
    23221 gaacagtgat atgactcaaa tcatggggga gtttattggg acattgatcc tagttttgct
    23281 tggagatggt gtgtgtgcgg ctgtgaatct aaacaaaagt aaagcacaag cttctggctg
    23341 gattgtcatt gcgtttggtt ggggactcgc tgtaacaatg gctgtctata tttctggatt
    23401 catgggccca gctcatttga atcctgcagt ctctctagct atggcgatga cagggtcaat
    23461 tagttggaat cttgtcgttc ctttcatcat tgcgcaagtt ttaggagctt ttgcaggggc
    23521 cattcttgtt tggctttctt atttgccaca ttggaatgca acgaaagatg aaagtgcgat
    23581 tttgggtacg tttgcaacag gtcctgctat acggaactat ccagcaaatg taatcacaga
    23641 attaattggt acttttgtat tagttcttgg gttgctagct tttggacaaa acgaatttgc
    23701 acctggaacg aacgtattcg ctgtaggtgg attgatctta gccatcggtc tttcattagg
    23761 aggaccaaca ggatacgcaa tcaaccctgc tcgggacttt ggtcctcgat tagcacatgc
    23821 ggtattgcca atagctaata aaggaacttc agattgggca tattcatggg taccaatcgc
    23881 aggtccaatg atcggagcaa ttatcgcggt aggtgtctat tcattaatgg tgtaaatcta
    23941 taaaacatac taaggtttga caatcaaaag gcgtcttgct atagttaagt ggaaaataaa
    24001 taagacctag aagaatgggg aatgttttgt gagtgtagct ggtatctaat cagtaagtcg
    24061 aatagccaat aaacaaggtt tatttggtgt gcttatgaaa cggtacctgt caggaaaaca
    24121 atatctttgt attctaataa ggataggaca tgactttttt aagctgtgtt ctatcctttt
    24181 atttatctct aggcccttat ttattttggg acaggtatct cttcaagaaa aggagagagt
    24241 atgaacaacg aaatgattag tcaattacaa tttgatcaga tcaaaaagga aatccaagca
    24301 agagcaatcg gaaactatag caaaaaacga atcagtaaga tgacgacctc gtcaaaccta
    24361 caaaccgtct tggatcgtca agaagaaaca agagaagcac gcttgatctt agaaagtagt
    24421 cagcatgttc catttatggg attgccgagg attgatgcac tgacagaaca agtcaaaaaa
    24481 ggattggttc tgtcgcccgc tgatttgatc gaatatgcgg attttctaag aagcagtcgg
    24541 atgatcacaa agttttttga taagaatcaa taccaggcac ctttgttatt tgcttatagc
    24601 aagcatcttc ctgatttgat aaatgtagaa gagttgattg accaaaaaat aaaaaataat
    24661 aaagtcagtg acgatgcttc acgtaatttg cgtaaagtac gcaaacaatt acaaataata
    24721 gaaaaagaaa tccagtcaaa attgctgaaa ttcttgcgtc atccgaaaaa caaagaaatg
    24781 atccaagaag caataatcgt tcaaaaaggt gaatgctata cgattcctat caaagccagc
    24841 tataaaaata aagtagatgg gacgatcatc gatgaatcga acaaaggaac gacggttttt
    24901 attgaaccga ctgtggtttc taaactgaat gaacactatc agttgttaaa agctgaagaa
    24961 atcagtgaag aatatcaagt tctggctgca ttgactggtg ctattgcaga aaatgaagaa
    25021 gcaatcgatt tattgatcga aacgatgacg gtattagaca tcatttttgc tcgggcaaaa
    25081 ttcagtcgag aaatcaatgg gatcacgcct aaaatcaata aatcagaaca tatcgtcatc
    25141 aaacaaggca gacatccatt tttacctgat catgcggtac cattggatgt tgaaatcggt
    25201 aaagactatc gaggcttgat tattaccgga gcaaatgccg gaggcaaaac agtcgtgttg
    25261 aagacagtcg gtttattgac tttgatggcg atgttcggta tgcaagttcc ggcaaaagaa
    25321 gggacagagc ttgctgtctt tgatgagatt ttcgtcgatg tcggtgacca ccaaaattta
    25381 gaaaatgcgt tgagtacatt ttctggtcat atgcaaaaca tcgctgctat tttgaaaaag
    25441 ataaaaagga atacgttggt acttttagat gagattggca gtggaacaga gccaaatgaa
    25501 ggggctgcac tagcgatcgc aatcatggaa tctatgtatg agcaaggggc attgatcatt
    25561 gccacgaccc attatggaga aatcaagaaa ttcgccggag atcatgaaga ttttgtgccg
    25621 gctgctatgg ctttcgatcg ggaagcactt cgtcctaaat atcagttgcg ggttggagaa
    25681 acaggagaaa gccaagcttt gtggattgct cataaaatgg atatgtcgat gaaattgatc
    25741 catcaagcag agcgttactt ggcggaaaaa gcctatcgga cacagaaaaa agaatttctt
    25801 cctaagacag cagttacaaa taggaaggag aataaaaaag aacgccagct ctttgcaaaa
    25861 ggtgatcgga tatttgtcaa tgaataccaa aaagaagcgt tagtttatga agatataggt
    25921 gaagatacga ttgatgtata tttagacaaa aaaataatcc acgtgcctcg tcagcgtgtt
    25981 cgtctggtgc gttcagcaga agatctgtac ccgacaggtt acgatttaga cagtctgttt
    26041 atcgattata aaacacgtaa acggcagcgt gacctagagc gaggatcaaa aaaagctcat
    26101 aaagtactag taaaagaaat gcgcaaacga caggaagaaa gaagagtaaa cgatgaaaac
    26161 agcaagtgat tatctcaaat tagctcataa tatcagtggc tgtaaagaaa aataaaaaca
    26221 gtgaaatcat tggttttatt ccccgtgtaa gctaaaataa aaagaaaaga gtgctgagga
    26281 ggtagggcga taaaatgaaa ttaagcacag actggcgtaa agaaatacaa acgattccga
    26341 atcttttatc gatattccgt atttttttgc tgccgattta tctttatttt gtgctgagac
    26401 agtcatttta tatagcagga gcggtcattg ttgtctcagg tttgtctgat tatctagacg
    26461 gggtcattgc tcggagatat aatcaggtaa cagacttagg gaaggtgctt gatccgtttg
    26521 cagataaatt gacacaactt tttctgattt tgtctatggc atggtatcga ccgtggcttt
    26581 ggctgttgtt tggtctattc ttgataaaag aaggctttat gtttgtagct ggattaatcg
    26641 ggctatctaa aaatatcaaa ttaagtggag cgaagtggta tggcaaagta gcgactgctg
    26701 tcatctatgt tggcatgatc cttttgctgc ttttcccaga attaccaacg ctgtgggtga
    26761 gagtcatttt tgccgtcatc acttacggtc ttttacaatc ttttgtcttg tatgctgtcg
    26821 agtaccgaaa gatgtttcaa agaaaataaa aaaggggtgt gacctatgtc gcattccttt
    26881 ttttgataaa tagactccca gtagctactt ctcgataata agccaaccaa accaaaaaca
    26941 ggagaagttg tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc
    27001 tcttggtaaa cggtgttcaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata
    27061 tgagcagcta tttttccgtt tgtttctcta atactcaggt caaaccgctg attttacaac
    27121 ctcttcctta aacggtattc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
    27181 atgtagaaat ccattttcac aaaatttttc ttaatactcg aaactaagcg actttttcac
    27241 aatctcttcc ttaaacggtg ttcaatcagc tgaccttcgg tattagctca aaaaacggca
    27301 aaatatgagg agctattttc ccgttttttt ctctaatact taggtcaaac cgctgatttc
    27361 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
    27421 cgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatgc aggacagcgt
    27481 tttcacaacc tttgattcac ggttttccag aagtagcttc ttcgaaaaat aagccgaaat
    27541 ttcttgaagc tgatcacttc tgtcgcaacc tctactgact aaggcttgat ataagcataa
    27601 ccttgggatt gcagttcaac taaatcaagg acacctgctg gtacaacgat tacttgttca
    27661 ggtaaagaac tttctgaaat atgattagca cgcatagcat tggcgcaagc gtgaaaaacg
    27721 actccttgca aatctaagaa ttctgcattg gcagagtcta gataaccggt aatcgcgatg
    27781 ccatttacag atacgacaat cgtcgtttct ggagaagctt ttatcaggtt ttttacgttt
    27841 ttccctgttt ctgaccattt ttctaattca tcaatatgga aaacgacttt cattgaaatc
    27901 attcctttac tttttaataa tcatcttgat cgcttgttct agtttagccg cttgttcttc
    27961 tggattacta tcaggtttct caaagcaatt tttcagatta tctgcaacga tcatgcccat
    28021 tacgcgatca atactagaac gaacagcgct taattgagtg atgacatcca tacattcttt
    28081 ttcgtcttcg atcatttttt ggataccacg aatttgtccc tctgtacgtt tcaaacggtt
    28141 caggatattt ttattatcaa tctccataac acattctcct ctatttttat aattagataa
    28201 taccccttat cggtatttta tgcaaatata aaaagtagtg aaaaaaatga agagtcattc
    28261 tggttgacat attaataaag ctcctgttta atatacccca tgaggtattt ataaaaatgt
    28321 aaatgaggtg cagaaaatga atttgctatt tcaatcaata aaatcgataa cagtcccaga
    28381 attaaaggaa aagcttttgg aaaatccagc gttacttgat gttagaacac ctgcagaata
    28441 ccggggcggg catataaaag gagctaaaaa tgttccatta caaagtataa acagatatga
    28501 tggagacaaa gaaaagaccg tctatgtgat ttgtcaatca gggatgagga gtaaacaagc
    28561 agctaaagaa ttaaagaaat ctggttatga tgtagtgaat gtacgtggtg gcatgaatca
    28621 atggtttgat cgaacagtag gagggaaata agatgaaaat cgttattgtc ggaggtgtag
    28681 caggtggaat gtctgctgct acacggcttc gccgattaat ggaagatgca gaaatcgttg
    28741 tttttgaaaa aggaccgtat gtttcttttg caaattgcgg cttgccttat tatctttcgg
    28801 gagaaatcag tgaacgggaa aatcttcttg tccaaacgcc agaatcatta tccgctcgtt
    28861 tttgtttaga tgtgcgtcca aatcatgaag tgacagccat ctttcccgaa aacaaaacgg
    28921 tagaagtcgt acatgagggt caaaaacaca ttgaacagta cgatgcattg gttttatctc
    28981 ctggtgcaaa accagttgtt ccatcgattc cagggataac agaagccgac aatgtttttt
    29041 ctattagaaa tgtaccagat atcgataaag tgatgcatgc attagaaaaa cagccaaagc
    29101 gtgccgtgat cgttggtgca ggattcatcg gattggaaat ggcagaaaac ctaaaaagaa
    29161 gaggtttaga agtcatggtg atcgaacaag caccacatat tcttccaaca ctggatgaag
    29221 aaatggcagc ttttatagaa aaagaattgt ctcatcaagg agtagaagtg attacttctc
    29281 atgctgtcgc tggatttgaa gaccacggga aacgattgcg actggatgat gggcgtacca
    29341 tccctgctga tttagttatt ttatccattg gtgttcgtcc tgataaccag ctagcagtga
    29401 ccgctggaat cgaattaggc atacgcgggg gtatcctagt agacgaacgt tatcaaacga
    29461 atattcctga tatttatgcg gtgggggatg ctatcgttgt aaaacagcaa atcactggaa
    29521 aagatgcact tatttctctt gcttcaccag ccaatcgtca aggtagacaa gttgcggaca
    29581 cgatttccgg aatttctcga agaaatcaag gcggtattgg aacagcaatt atacgaacgt
    29641 ttggaatgac tgccgcatcc accggtttaa gtgaaagaac agccaaagaa aacgaactgt
    29701 cttttgaagt cattcatgta tcaggaaaag atcatgcaag ctattatcca gaagcaacag
    29761 atattttact gaagttgatc ttccatccag agactggcga gatttatggt gcacaaggtg
    29821 ttggggcaaa aggtgtggat aaacggatcg atattttagc aacagcaatc aaagggcatt
    29881 tgacgatctt cgatttaccg gaattagaat tgacgtatgc accgccattt ggctcagcca
    29941 aagatccagt aaacatgcta ggatatgcag caatgaacat tgcagaaggg cttagtgaaa
    30001 ccgtacaatg gcatgaattg ccgacagaac tagcaaaagg aaaaatttta ttagatgtgc
    30061 gaacagcaga agaattggaa aagggcaaat tcaaggaagc caaacatatc cctttgaatg
    30121 aacttcggga ccgattaaat gaattagaca gccaacaaga atatatcgtc agctgtcata
    30181 gcggattacg tagctatcta gcggaaagaa tcttgaagca gtctggctac cacgtaaaaa
    30241 accttgatgg tgcattttct ttatatcaaa ctgtccgacc tgaagaactg atatatccta
    30301 acaaatgata ctcgttaaaa agcttgcatt cgactcttaa aagaggtaaa ctaatcggtt
    30361 ccaccacttt aaaaaaagag gagaatgtgt atggtcaaca cacaaaaaca gttagaagaa
    30421 gcatatctga agaacgtgta tcaaaagcta attgataaaa aaaacgaact gcgggaatcg
    30481 atggatacgg caaaaataga aggaaaccag tcgttgaaag aaatggctgg cgagcttcga
    30541 ttgaattttg acagttactt agataacctg gatacttttt cgatgattga aatgaaaaat
    30601 agagaaatcg atcagatgaa tatcaaacag aaaaacgcag aaaatcagct agcaaagatc
    30661 gaacgtttat tgaaaagtcc ctactttgga aaagtcgttg tagactttct ggataatgag
    30721 ccaaaagaat ccttttatat cggtacagca aatttcacag atgatgcaga tgaaaatctt
    30781 gtttatgatt ggcgttcacc aattgccgaa cttttttaca ataacatgat tggtccatct
    30841 tcttatacgg ttcgtcaaaa taaaatcgat acggttatta aagaaagacg acaatttctt
    30901 ttggaaagag atcgtttggt caagtttttt gatacgtctg tagctattca agatgacgta
    30961 ttattagaag tcttaggcca agatgagacc aatgaaatga aggctatcac tgctacgatc
    31021 caatcggaac aaaacacaat cattcgggat gtgaaaagtc aaaatatctt ggtaaatggc
    31081 gtagcaggaa gtggtaaaac ctctgctatc atgcagcgta ttgcgtatct gctctattta
    31141 tatcgagata cgatgacatc cgatgacttg ctgattctat cacctaatca tcggtttatc
    31201 gactatattt cgaatgtcct accatcatta ggtgaaagaa atccactgaa tctaaccatc
    31261 atacagctgg tttcacagct ttcagcagag gaaatagaag gtgaagaggc ttatttcaaa
    31321 cgtatcacgg gagaaaatgt atcggagcag acagaacgtt tgcgtagcaa aaagtttatc
    31381 gataatctaa aacaatcaga tcctttgttt ttagatcatc cgaattttat ccgtggactt
    31441 actaaaaatg gaaaaacagt cctttctaaa aaaacgatcg aaaaaatata tgagaaagtt
    31501 cctgctcatc ctaaattgat cgataggctt caagcaacga aaaaggcact gatgagcgaa
    31561 tggaagaatc atcttttgaa acaagcaaaa agtcctgctg tgcagaatca ggtactctcg
    31621 ttgacagaag ataggcagtt ggaattattc ggtaaattga tttctgatga ttcggaacaa
    31681 agtattgtag cttatgcaag gaaactttta caaaaaaaat atcgaaaaat aaccagacaa
    31741 atcgaagaaa tggcatgggt ggatgagcat cagttgtttg aaagaattta tgaaaaacga
    31801 tatggctcag cttatgcttg gcaacccaca cgtactgttg atgaagcagt cattatatta
    31861 gctattcgtc atttacttgt tgaaaaggta aatgtaccag catttcgcta tctattgatt
    31921 gatgaagtac aagattatac cctggcacaa ttaggtctat tgatcgaatt atttccaaaa
    31981 actcacttta ctttagtagg tgacgagaat caggcaatct ttaattcttc cactactttt
    32041 gctgatatta tgcgttgttt cgatgactac catctgccga tccatcggta tgatcttaga
    32101 aatagttacc gttctagcgg agcaatcaca gagcttttca aaacctatgc tgtggatcaa
    32161 gagaagatcg acatcgtatc gatccgaccg caaggaaaag aacccgaata ccgctctttt
    32221 cgatcctctg aagaattgtt gaaaatacta gctgagattt tggtttctct aaaaggagaa
    32281 aaagcggcag ttatcaccca aacagaagag gaaacccaaa cgctacaaga atcgattaaa
    32341 aaaaattcat atggattaga aaactgccaa atcattcctt tgagtctagc aaaaggactg
    32401 gaatttgatc atgtaatctt atatccattt gaaaacgatg gagatgagca aagaagacgc
    32461 agacaaatgt acacggcgat atctagagga atgaaaagca ttgtcgtatt ggaaagagca
    32521 acataattaa gagataaaaa ataaaaagaa caactgataa aaagaacaaa atatttttca
    32581 tttgctgatg agaaatgttt tgttcttttt cttaggaaaa atttaggaac actttatgtg
    32641 aaaagaaatg aaatgtatgg taaactccta cgtcgtaaca aaaaaacgtg aggtgaggat
    32701 atgaaaaaag taacaagaga agaagttgca tcaattttgg tgaaagataa gtatttttca
    32761 aaaggtaatt actggttaaa gatttggcaa acgttagtgg ctcttttagg atggatgatc
    32821 gttgttcttc cttttatatg ggtctttttc ccattaacga gaccagaaag agccaaggat
    32881 ttttccttgt acgctttttt atcagaaaag aaaatgctgt atttcttaat cggctttttt
    32941 gtattgattt ttttcagttt cctgattacc tcatttgtat tgactaggtg gaacaatcga
    33001 aatctataca aaaaagtaaa taaatctttt gaatacgccg tttgtataat taagcgagac
    33061 aaataaaaaa gccatttatg ttacactc
//
LOCUS       DAYYNG010000015        32384 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000015 DAYYNG010000000
VERSION     DAYYNG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.015"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACHBFA_000572"
     CDS             6..245
                     /locus_tag="ACHBFA_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394092.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACHBFA_000573"
     CDS             312..521
                     /locus_tag="ACHBFA_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394093.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACHBFA_000574"
     CDS             581..1252
                     /locus_tag="ACHBFA_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9394094.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACHBFA_000575"
     CDS             1305..3281
                     /locus_tag="ACHBFA_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9394095.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACHBFA_000576"
     CDS             3294..4049
                     /locus_tag="ACHBFA_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9394096.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACHBFA_000577"
     CDS             4046..4870
                     /locus_tag="ACHBFA_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9394097.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACHBFA_000578"
     CDS             4880..5128
                     /locus_tag="ACHBFA_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394098.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACHBFA_000579"
     CDS             5171..7270
                     /locus_tag="ACHBFA_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM9394099.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACHBFA_000580"
     CDS             complement(7308..9365)
                     /locus_tag="ACHBFA_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFM9394100.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACHBFA_000581"
     CDS             complement(9479..9691)
                     /locus_tag="ACHBFA_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394101.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACHBFA_000582"
     CDS             9878..10345
                     /locus_tag="ACHBFA_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394102.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACHBFA_000583"
     CDS             10342..10584
                     /locus_tag="ACHBFA_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFM9394103.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACHBFA_000584"
     CDS             10602..10904
                     /locus_tag="ACHBFA_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394104.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACHBFA_000585"
     CDS             10976..13030
                     /locus_tag="ACHBFA_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394105.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACHBFA_000586"
     CDS             13030..15642
                     /locus_tag="ACHBFA_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9394106.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            15639..18017
                     /locus_tag="ACHBFA_000587"
     CDS             15639..18017
                     /locus_tag="ACHBFA_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM9394107.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            18078..18620
                     /locus_tag="ACHBFA_000588"
     CDS             18078..18620
                     /locus_tag="ACHBFA_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394108.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            18698..19030
                     /locus_tag="ACHBFA_000589"
     CDS             18698..19030
                     /locus_tag="ACHBFA_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394109.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19031..19624
                     /locus_tag="ACHBFA_000590"
     CDS             19031..19624
                     /locus_tag="ACHBFA_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394110.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19646..21340
                     /locus_tag="ACHBFA_000591"
     CDS             19646..21340
                     /locus_tag="ACHBFA_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFM9394111.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            22028..23905
                     /gene="ltrA"
                     /locus_tag="ACHBFA_000592"
     CDS             22028..23905
                     /gene="ltrA"
                     /locus_tag="ACHBFA_000592"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9394112.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            24036..24413
                     /locus_tag="ACHBFA_000593"
                     /pseudo
     CDS             24036..24413
                     /locus_tag="ACHBFA_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24413..25618
                     /locus_tag="ACHBFA_000594"
     CDS             24413..25618
                     /locus_tag="ACHBFA_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9394113.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25634..26278
                     /locus_tag="ACHBFA_000595"
     CDS             25634..26278
                     /locus_tag="ACHBFA_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394114.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26289..27095
                     /locus_tag="ACHBFA_000596"
     CDS             26289..27095
                     /locus_tag="ACHBFA_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394115.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27117..27347
                     /locus_tag="ACHBFA_000597"
     CDS             27117..27347
                     /locus_tag="ACHBFA_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394116.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27893..28168
                     /locus_tag="ACHBFA_000598"
     CDS             27893..28168
                     /locus_tag="ACHBFA_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394117.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            28210..28632
                     /locus_tag="ACHBFA_000599"
     CDS             28210..28632
                     /locus_tag="ACHBFA_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394118.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28651..30243
                     /gene="mobP2"
                     /locus_tag="ACHBFA_000600"
     CDS             28651..30243
                     /gene="mobP2"
                     /locus_tag="ACHBFA_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM9394119.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30255..30590
                     /locus_tag="ACHBFA_000601"
     CDS             30255..30590
                     /locus_tag="ACHBFA_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394120.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30737..30931
                     /locus_tag="ACHBFA_000602"
     CDS             30737..30931
                     /locus_tag="ACHBFA_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394121.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30963..>32384
                     /locus_tag="ACHBFA_000603"
     CDS             30963..>32384
                     /locus_tag="ACHBFA_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM9394122.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKK"
BASE COUNT        11937 a         5011 c         6595 g         8841 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatt gtgaaatact ggaagcgagg agacatagac
    13861 accgcaacac agttaaccaa tacttttacg aatacgattt attatgatgc aaatgcttca
    13921 gacaataaat actttaacga aaatgcgcag aaagctgtca atgcgcttat ttttgcgtta
    13981 ttggaacaag cggatcaaac aggggattac tccaaattaa caccaaataa tctcgttgaa
    14041 ctactttcag aaattggcgg ttttaattat tatccagatc ctgagaatga attcaagcag
    14101 ctcaatgcct tagatgagtt catgaatcag ttaccccctg gaaatatagc aaaaaaacaa
    14161 tatggggcaa caaaaattgg atctgataaa gcgaaaggga atatcttgtc tacggcaatt
    14221 acaggattga atccatttac cctaacgaaa attgcgaaga tgacttctca atcgaccatt
    14281 gattacaagt ccattggttt tccgaagtat ttggatctca aattacatga aagcctatta
    14341 aatcaacgca ttgtcgtgga attttatcat aagggaaaga aatttcacga ggaacgagtc
    14401 aaagttggct atcgtggttt ttgtgaaatc aactttgatt gtcatttaga gcagggagat
    14461 catgtttgct taaaatacaa tcatcaagga aaacaatatg ttgcttggta tgagttttct
    14521 caaaaagtat tgaaagacga agagggaaaa atcgtttatc gaaaaaaacg aggagaaact
    14581 catttgccag aactagaaaa agaagccgtt ctaacattag atgagaagaa aagtaaccta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgttaacg
    14701 cctgattatg atccatctaa ccatatcatt gttagcatct ttcttagtca gttgtataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgtag gattcatttt
    14821 attttagatg aatttggaaa tatgccaccg atggacaaca tggaaggaat catgacggtt
    14881 acggcaggtc gtaatatgtt atgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg tgaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcaacggat
    15541 cgtcttcaag aatatgttga aaagttgatc aaaaatgaag aaatttctga aatgattagg
    15601 aaacggctgt tattggaagc tgaaaaggcg gtgaaagaat gatcattgga ggaattttct
    15661 ctggcatagc cgactggtgg tcaggtttat tcggagaagg ggatgcagat cccaaaaccg
    15721 taacgaaagt cttacaagat tatggtaatt atctgcagta tcaagatgta ttcagttgga
    15781 tttgtaatac ttttttatgg ggattgatca aagggttata taatctcaat actttattag
    15841 aaaaaacaat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggtgttaatg
    15901 aactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccgaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aaacttttat ggagacgtca ctgcagcaaa taaagcagat aaaacgggtt
    16141 cacctgcttt tcaaattatt caaaataatg tgattgatgt cagtacatta ctagcgactg
    16201 acccgaataa agtagaatcc ttaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 tcaaaacagc gaatatcaat gggattatta cacctgatca agctaagaaa atgtctgaat
    16321 caagtgatat tagtacagta caaaaagatc ggttaaaggc attacagtat cgactagagt
    16381 tagacgataa tgggaaagaa atccctgttc aaattagtga tgaaggttta gcaaaatatg
    16441 tgtatacgtc aggctatcgt cgatacatta gtagtccagg gattattttg gctggggaat
    16501 cttctttgtc ggtagcctat ttatttattt tgtttacaat cgttacttgc attattgagc
    16561 tagtatttaa aaaattctat ttagtaattg ccgcgtcaac ggactttgag acaggacaac
    16621 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    16681 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggtaggaa
    16741 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    16801 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagcaatt
    16861 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    16921 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aagaatggat
    16981 ttggccatcg aaaagatatt acccatgata acggggaaga acaatcagac gataaagcta
    17041 caactgtacc taatagtaag aagacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    17101 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagttg
    17161 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    17221 aagaatttgc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    17281 cttcagcagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
    17341 tgaaggcaaa tacaacaagt gaaaaagaaa atcaagaagg tgcagcgatt ccttcagcca
    17401 aagatcacca agatgagtta cattctaacg aaaattcgat cggagaaaaa gattcagaag
    17461 aaggacaaca tggtcagtta ccaaaaaccg atccagaaca atatcgttat aaatctaaca
    17521 ttaattctga aaacgaaaaa cgcatttcag atttaccaaa acgaaatcaa gaagtacaaa
    17581 cggatgagga ttccatcgat ctgccaaatg ataatggcat cgaagttgga actgaaaaat
    17641 caacgaaaat tggtttgcct aagacacaaa atttaccaat gaaagatagt cgtttgaatc
    17701 aattcaaaga gaatgatcaa agctttgttc atcaatcacc tgcaggtact gttcaaatga
    17761 aatcaagtcg tttgcctgaa aatggaaatc tgacaactgt ttcttacaat ttagctgaaa
    17821 gtggtgaaac agagaatgcg tccgttcaaa atattgtgac aaaagaagct gcacctaagc
    17881 caatcaataa agttcataat ggatacagac cagcaaacgt agcatttact gcaccgacaa
    17941 aaataaatgt gacaagagaa actaaaaaca ggaacaatga attgaaagaa caactattga
    18001 aggatatcaa accataaaag gcctatttaa gggccttttt ctttataaaa caagtttaaa
    18061 atggaaagga gaaaattgtg gaggatattt taatagctac gtttataact gcactgggag
    18121 catttttaac gatatttgtg gtgactatta aaggatttaa agatacggat agtttacaaa
    18181 aagatcatca gtctatttta gatggtcaaa aaacacatac cgaaagcatt attgtacacc
    18241 tttcagaagg attcacttat ttgaaagatg ctatgcccac attcacagta aaattaacgg
    18301 aaatatttca ttctgttgag caaatatcgt cagaattgaa agagcaaaga caacaaaaag
    18361 atcgtcaact tggctcactt aattttaggc aagaaacgat acaagaatca ttggagaatg
    18421 taaatcgatt aaacgatgaa atgcaacgag ttcatttgga aaatgaagaa ttacgcgcac
    18481 aaaattatga tttaattcaa aagaaaagaa atctggaaca aaaaattgtt gaattaaaag
    18541 aagaaaatcg tcaattgaat tatcaattac gacgaggaaa aaatcgttct cgtgatttag
    18601 gaaatgaatt agagttgtaa tatttagaaa aacgctgtga gaaatcacag tgttttttat
    18661 ttctaaaaat acaaaatata aggggtatgg ttatattatg gataaataca ttcatatgaa
    18721 aaatcctcca agatttagtg gggaatggaa gtttttcgcc tttttctttt tagatctatt
    18781 aacgcttggt ggctgctttt tttgggcgaa aatagtcaat aatcttgtcc acttaccact
    18841 cttttttgca ctcttgaatt atggattatg gcttgcgata gcgttagtga ttgtttggcg
    18901 accctataat aaccctggtc aacgccaaat ttttgcgatt atcgaatcta ttttctttac
    18961 ggataagaac cactatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19021 ggatgaataa atgcgagtca aaggacaaaa ttcagttgat aagaaatcca ttttgcagtt
    19081 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19141 gggagtctta attactagag aagatcattt tcagcgttat tatcgtgtcg tttccactga
    19201 cgtggaggga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19261 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19321 acaaatcgtg caaaagcggc agttattaga taaaaatcgt atggaacagt cactaggaag
    19381 aaatctacag cgattaagaa aatatgaaaa gaaattagta gaggagatcc aggaattaaa
    19441 acgggcagaa caagaacgtc ctgacttaag cttctttttc gttattgaag gaaagacaat
    19501 caaggattta caaactagaa atcgtcagtt aatgcgatct agtggcattt tagggttaaa
    19561 accactctac aaaaaagaat tgattaagat tctttatcga atgaacaaca tgaatgacga
    19621 ataaaaaaga tggaggaaat gattcatgct agatgtgata aaaacagctt tttcaacgca
    19681 aagtaatcaa aaggtgattg atccattaaa acaacttggt aatcgtgaaa tagagatttt
    19741 aaaatcaaaa ggttatgatt tagatttctt aaaacaagtg atgccacaag gtgggttgaa
    19801 ttttgatgaa gaatatgtga cctatggaaa tggttattca cgatgtgtga cagtttatca
    19861 ttacacaaaa aatcctactc ttttttggtt ggccaatctc atgaacaatc ctcatacagt
    19921 ggccacacta gatgttagca cagatgaaaa gcaaaaagtg attgatcaaa tcaaccgcgc
    19981 attagatgaa ttaaatgatc gatcagataa tgaacgaaaa gcgactgacc gaaataaaag
    20041 tgcggaagaa tacctggaac tagcggaata tgcccgaaaa ttgacttcta atggggaaat
    20101 ttctaaacaa gtcaaagtga gagtacatgt ttacggaaat acgatccaag aagtggatga
    20161 acgggcgcag gaactgatcg aaaatctaaa agggatggat tatcgtgcgg tgacttatct
    20221 gcataaaacc aaacgagatt ggcaatcgat gtttaccatg ttcgatgaac aagctgaatg
    20281 gtttggaaat atcaaagggc aatcacttcc atcgttaaac attggcggag gattcccgtt
    20341 ccaccaccaa acgctaaaag atccaggagg cacacaatat ggggaaacca tgacaggcgg
    20401 cccatttgtc tggaatgcca cgtataaaaa tagtgtacgt ttatctacca ataccttgct
    20461 tctaggaatg atgggagctg gtaaatcgac cgttcttaaa atgatcgcgg aagcccattt
    20521 agctgcagga gactttattt ggggctttga aaagggaaaa gactttattc ctttcttaaa
    20581 ggaatataac ggtattatgg tgcgtttaga cggatctgac ggaatgatca atcctttaga
    20641 gatatttgcc acacggacgt atgatgaagc atccagttta tatgacgatg gatcagtcaa
    20701 agatttgaag atcaatgaag cagcgtccta tcaaacacac ttagacaaag tggtctatca
    20761 ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa gcagaattta agacctactt
    20821 aaatcgtttt tatgaagagt atggaacagt accacgcggc tttacttcct caaattcgcg
    20881 ttcaaatact gatacgcaag tcacagggaa agatccagaa gcttatccga cgttcaaaga
    20941 atttttagcc tttttaggac aactggaact accaggggct tcccaagaga aaaagaatcg
    21001 gaaagaagaa atggagtcta tcgtggaatc cttatgtgag acttatggca tgatttttga
    21061 tggtcatagt acgattcgac accttgacca acaacagttg gtcttttttg acattgactc
    21121 tattacaggc ctagatcgag gggtacaaca atgtcagatg tacatggcca tgacgttgat
    21181 ttggaatcag gcattgattc aaggacgaag acaacgacgc ttaattaaag aaaaacaaat
    21241 ggaagaacaa gacttcaaac gcttcttggc gatgattgat gagtgccata atggtgcgac
    21301 ctgttctgca gtgaaaagct gtgggttgca agtagtctaa aaactattgt aataactctt
    21361 ataacgagaa gtcaaatgag tgggtaacgc cacaaagggc ttccactgat actccgacat
    21421 gcaacaagta gtttatgcag accaacgttg tgtgaagctc ggtgaagtcg gctgaaggat
    21481 accgtaaggt aaaagaaata gtgaatacca cgaaagttct ctagaggtaa agggtgtatc
    21541 cgataagtcg ggtgtccaaa aaaacttaat atggtgagta tgtacaaacg tactgacgaa
    21601 ctagcgaatg tactgtccga aaggcatagc tcctagaaat gggagagaca gtgagcaaaa
    21661 aatcactcat tgtgctaact tgtggttgag taaaagtcgt tgttatgaaa gaccatgctg
    21721 cgttacaggc gtaggagaag ttagggggca aaagctagca tctaagttaa ggcatgtgga
    21781 tagatataag ggaacagggg aagacatcaa catggagagt gcatactcga tgaagtggta
    21841 gtaagaaata tgtgaatgac aaataactta ctttagtgat ggtgaaagta gagtcacgac
    21901 tgatgaaggt tctgtaatgg aactggagga acaggctcga ttcgtctgta aaagtcatga
    21961 aacccgttca accatgggat tctcgtatga ctaaaaaggt cacgagccaa ttggagcgtg
    22021 ataaaccatg acaaaagaaa caagtccaaa agataggaag ttgcggcatg cagaatatta
    22081 tggcatgacg gaaatcttcg ataagatata ctccaaaagt ttgaagggag ataatttcaa
    22141 gaatctaatg cagattatca tttctgataa caacattgtc ctagcatatc gtaatatcaa
    22201 gagaaatcac ggtagtgtga caagaggaga agatggagta acgattaaag atattgaaaa
    22261 tctcaaccgt aatgaattcg tagaaattgt taggaagcga ttcgcacatt ataaccctcg
    22321 gaaagtaagg cgtgtagaaa ttcctaaata taatggtaaa ttaagaccgt tgggtattcc
    22381 atcaatatgg gatagaatcg cgcagcaatg catcttacaa gtattagaac caatatgtga
    22441 agcaaaattc aataatcaca gctatggttt ccgcccaaat agatcagcag aacacgcaat
    22501 agcagattgt atggcccgaa tgaatcaaag tcacatggaa tatgtggtag atgtagatat
    22561 caaaggtttc ttcgacgaag tcaatcatac taaactcatg agacaaatct ggtctatggg
    22621 tatacgagac aaacagcttc tcttaatcat taggaaaatg ttaagagcac ccgtagtact
    22681 tccaaatggg aagttgatat acccaacaaa aggaacacca caaggcggaa tacttagccc
    22741 attacttgcg aacatcaatc tcaacgaatt tgactggtgg atagcaaatc agtgggagga
    22801 aaaagatgcc aaagagctaa aacatcattt taggaaaaat ggagcttggg ataaaggtaa
    22861 tatctatcac aagctcagaa agagtacaac tctaaaagaa atatacctag tgagatacgc
    22921 agatgatttt aaaattttct gtagaaatta taatgaagcg aaaaaagttt tctatgcaac
    22981 taaaatgtgg ttacaggagc gtctaaagtt accaatatct gaagaaaagt caaaaatcac
    23041 aaatctttca aaagaagaaa gtgaattttt aggctttacg atgaaattag tgaccaaagg
    23101 taacaaaaag gttgttaata cgcatatagg tacaaaagca caaagcatga ttaaagtgaa
    23161 actaaaaaaa caaatcaaac gaattcaaaa aagtcctaat agcaaagata cctttatcta
    23221 tattggagtc tataacagta tggttattgg gattcataat tacttcaaaa ttgcgactca
    23281 ttgtagtgta gcaatgcgaa aaattgcaag agaactcaga ccaagttttg aaatacgact
    23341 acgagaacaa ggaatctcaa aagaaggaca atacggaaag tacgaaggat acaagcctta
    23401 cctacggtca ggacaaatac gatatataag agggtttcca atattaccag tagggtatat
    23461 tcaacacaga catccgatgt gtaaaaaaag aaatattaat aaatacaccg aagaaggtag
    23521 ggaagccatt cataagaagc aacaaaccgt agaagaatgg aagatatcgt ggttgtgtag
    23581 caatccagtg cttagtaaga gagcaacgat tgaatttgcg gacaatcgaa tttcaaaatt
    23641 catcgctcaa aagggaagat gtgcagtaac aggtgaggag cttatcttat ctgaaatgca
    23701 ttgtcatcat attatccctt atcatgaaag caaaagtgat agctatgaaa acctagtgat
    23761 tgttactgag gaggttcatc gggtaataca cgctacacaa agtgaaacga ttgaggaact
    23821 tctaaaatat ctaaaattaa atccaaaaca aaaagaaaaa ctcaatgagt tgagattaaa
    23881 agttggtaac gaagaaatat cttaataaat atttgtagga cgggaaaaca gaatgtcttt
    23941 tatagacgag ggaacgccgt atgcggtgaa agtcgcacgt acggtgtgga ctgggggaaa
    24001 agacggagat aatatcaaac tcttacctat cagtatcgat taatccagat tttattgaaa
    24061 cggtaaatta tgtcacaaac tttcaacgag aaatgcgtaa agtagaagca atgactgttt
    24121 tagctacaca atccccacaa gaaatggtgc cagaaaatat gtcttctgat aacttctcga
    24181 aactgaagaa agtctttgaa ttttcttcta cgaagatctt tatgcgtatg gatgaatcta
    24241 ttttacgtca tatagaagga ttagtgggta aatcaatgac gaactcagaa ttagaatcca
    24301 ttcctcaaca aaatcaagga gacgctgtga tcaattttgg ctcaaaagaa accattcgtg
    24361 ttcattttag tccgaacaaa cgtcaattaa aattgtttga tggaggaaaa taatggaaaa
    24421 ccaaaatgaa agtttaatca agcagtatgt taagcgtcgt gcaaagcggc gcttattttt
    24481 gtggttgttt ggtacttctg caggtttgat tactattttg atcactgtgt tcgtgaccct
    24541 atttctaata cttgcggcgg gctcgattga taattcggat tcagattcaa gcagtggtgg
    24601 cgaagctttt acaggagaat attcggaagg cttaccgatt tataaagaaa tcaaaggaag
    24661 aggccctttt tctgatgaga ttgcgcaata tgccgtaggt gcagcagtga aatataaatt
    24721 attaccaagt gtgatccttt cgcaatacgg ttacgaatca gcgtttggta catcggcgtc
    24781 cgcaagaaat gatctcaact attttgggat tacttggttt gatggttgtc tgtttcctaa
    24841 aggaactgca cgaggaattg gtggcattga aggtggctgg tacatgaagt ttcccaactc
    24901 aaaagctgca ttttcctatt acggctttat ggtagccaca caatccaatt ttaatgcttg
    24961 tgtaggaaat aaaagccctg gcgcaagctt attgatctta ggacgaggcg gttatgccgc
    25021 tgcaggaatt acggaagata gtccgtatta tacaggttgt atgtcaatta tcacttctaa
    25081 taaattaacc gaatatgatg aattcgccat caaacattgg ggtgaaggtg gcaataacaa
    25141 cggcacgatt actggtgaat ggacgaatcc attccctggc agcagtttag ataaaagttc
    25201 cttcagcggt ggtcaattat ttggaacgaa tccaggcggt gaattccgtc cgaacggctt
    25261 ccatgatgga ttagattttg gttcagttga tcatccagga agtgagattc atgcggttca
    25321 tggcggaaaa gtcgtgtacg ttggcaatcc tggtatttca ggtttgggcg cgtgtgtgat
    25381 cgtgattaat tacgatggct tgaatatggt gtatcaagaa tttgcaaata gtacaggaaa
    25441 ttctcgagtg aaagtaggcg atcaagtgaa agttggtcaa gtaattggga tacgagatac
    25501 agcacacttg catttaggat ttactcgaat ggattggcgt caagcacaag gacatgcatt
    25561 cattgatgat ggtacatgga tcgatccgtt accatttttg aatagcagta aaaaatagat
    25621 caggagaaaa aatatggaaa ataaaacgtt aaaaatcgtc ttaacagctg caattagcgt
    25681 tagtttaatc ggcaatattg cgttgttttg gcagctgaaa gaggaacaag cagcaaatac
    25741 ctataaaatt gaagaagtgg ctaaaaacgc gtctgagaag ctcaaaaaaa attcctctga
    25801 ggcacaggcg aagatcgaca agttaaatca aaccatcaat gagaaaaacc aagaaatcga
    25861 tcagctgaag caacaagatg gcacaaaagc aaaagaggaa gtaactgaag cacaaagaaa
    25921 gacggcagaa gagttcggtc aattggccat tgataaatca ttaagcagaa atgaattaac
    25981 ggaaaaacta aaagatgtcg caacccaaga agtgatcgac aaattaagtc ctacagacga
    26041 tgatcaccaa catgatgatt acggaagtta ctcctttaca ttaggtgacg tgcaaactta
    26101 cgctcaaaca agcagtgtaa aagaggaaca aaattttgtc gtatttgttg attatacaat
    26161 cggcaatccg caattcaaag atgtcaaacc acaaaaaata aaaggtggat taactgtcac
    26221 tgaaaaacaa gtagatggtc aatggaaagt catagacttt agttatttca ctcgttagga
    26281 ggaagaaaat gagtaagaaa aaaggagaaa gacaacgttt caaaaaacga aaaagttatc
    26341 ttagatggct gggaatcttt atgattctcg gctttttgat tcttgctagt ccgatttata
    26401 cgaatcagaa ggtagatata ccagatgagg taagcacgaa cgccttttta aacacgaaag
    26461 agctgacaat ggtttcaaaa gaaaaaggag cgaaccagca attagttttt gaatttgcga
    26521 tcgatgatga agatcaaagc ttgttaaaag aactggctaa tcttaattat cgcgtagaag
    26581 taaagacagc taaaggggat tatcaagcaa tacgagccaa agtgattaag gtgtctgatg
    26641 attattttgc agtgaagcta acgcatgtac cagaagaata tatcgccatg cgtttaacca
    26701 ttattcctga aaaaattgat ccaaaagtgg atatgcaaga accacaagat ctgatttatt
    26761 acgtgcacga agataaggtt aaagatcaag tgaaagatga agattatgaa caacacgcga
    26821 tcaattacaa agtgaaagga tacaagaaag aacaaaaagc agctcaaaaa caaatggaat
    26881 ctttccaagc aaccattgat ttgaatgaag aattagtgaa gaagctaaaa gatcaattgc
    26941 cttatcaagt cgcagatgat caggaaaata ctgaaaacaa gattaatagc taccagcaag
    27001 aaattgaaac atcgaaaacg caactgaaag atcaagaaga gatcattcaa aaacttcaag
    27061 aaaaaattga tcgtatgaca aaagaaaaga tttagaagga caggtggagt gaaaaaatga
    27121 agcgaacaaa cttggctgac aaagatactt tcgctgagca gaaagtattg attaaagcct
    27181 atctcaaagt tgtaaaagct aaagaaatat gtcgagaagt gaatttatct aacacaacaa
    27241 tatctgagtt gaagaatggt cgaaagaatt tagatacttt aaaattcaga tcatttgaag
    27301 ctctatatgt agctgctaaa aaatatttta cagctgataa caaataaaaa acaggagtat
    27361 tttatgtgaa aaaagtatat aaaaaatgct tatgaatcag ctatatcacc ttaaccattt
    27421 gttaatgaga tgggagaaaa cattaaccac gattctaacg caaacatcaa gcacataaaa
    27481 catttaacac tcctcctttc tggctgaaga tttcaaccaa acaggaagac agcgtatcaa
    27541 ttatagcata cactttttct taaattcatt tttcatatga caaaatattt ccttctaatc
    27601 gacaaagtta gaaggatttt ttattgtcta aatacacaaa taaccttaat ttttaaagag
    27661 cataaggaaa cgtgatatca ctttctcacg aaaggtgtta ggtaggctgt gcctaccacg
    27721 tttctaagaa agataaatga tatcaaaatt ttggatttgt gtgtagattt gtgggtagga
    27781 tgaaagcgaa tgtgggcagt aaaataagcg tttgtgtgta gcaaaatcaa aaaatgtgta
    27841 gggatatgtg tagctacaca tatccctaca cataataaat ttggaggaaa atatggactt
    27901 attaattaga aatattgaag aaaagtatat taataaaatt gacaaacgtt gtgaagaact
    27961 tacggtcaag actggcaaaa aatggagtcg aaatcaatac ctaaaagttt tgatcgaaaa
    28021 tgattttgat cacgctcttc tgaattataa aaaagaccaa tttgatcggt tgttagaaaa
    28081 atttgtggat attcaaactt ataatacgaa aactttagaa gaatatattg caacaaataa
    28141 tcaattaatt ggtttgttaa ttgaataata aaaatagtat tgtgaattta taaaaggagg
    28201 aaatcaattt tggcaaaagc aacaagaaaa catgtatatc tttacgatat gcatatcgaa
    28261 gtacttgatg aaataatttc tactcatgaa gatatatcta attatagtga agcagtcaga
    28321 catttgtgta tgatgtatga tccaaaaatc gaagaaaaaa taaatccaat tcaaattaaa
    28381 ttgaatgcaa tgggaaaaga aatttctatt ttaaccgaaa tggtttcaga gtttgcggat
    28441 cttcatttga aagatacagg aatactaatt ggtacaaatt caactgttta tcaagaagca
    28501 gaagaacgtg tggaaggaaa aattaaatcc aatcaaacca aaaaatattc ttctaagtta
    28561 aagaaaccag tgaatgaatc tgttcatacc agagtagatc catttgatcc aacgaagatt
    28621 ctaaaaagat aaaagtagaa ggagaacgtg atgaataaat cgcctgcagt tattcttact
    28681 tgtcaattta ccttgcccaa taaaaaatct ttttcaacat acattgatta tatgacaaga
    28741 gaaaaagcac tggaagaaat ggaacgacgt acgcccgaag aagaacagga actgcaacga
    28801 atcaagaatg ctttagagga tttttatatt cctgaaggag aaactttttc ggaaagtaag
    28861 acggataaag aattcagtga aacgtcgaat gaagctcaat cgttgatgga acatggaatc
    28921 aattttgaca agttagagga acaagatttt accaaatatc tttcttatat gacccgtcac
    28981 tatgcgttag aacaaaagaa agaactgacg caaagggaac aaaaagaata ccaacggttg
    29041 aaccaagcaa tgaaaaaata tcaaacagag ccacataaaa aagcttcagg aaatcttcct
    29101 ggagtttttt ctatggcttc agacgaagtg aaaggaagtg atctgaagga gatcaaacaa
    29161 atcttccaaa gaggacaaca aaaaggctca atcatttatc aagatgttat ctctcacgac
    29221 aattcgtatc ttgaaaaaat gggcttgtac gatcccaaaa cagatacctt agatgaagaa
    29281 ggtttgaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga acaattgaat
    29341 gaaacaggtt actggatggc aacgattcac cgtaacacta agcatatcca tattcatttt
    29401 gcagttgttg aaaaagaaaa tacgcgtaaa ccgtatactg agattgaaaa tggtgtgagt
    29461 tatatcgtac cgaaggggaa gagaagacaa gcaacattag acgcaatgaa aaccaattat
    29521 gtacatgagt tagaacgttt ctcttatgaa aaggatcgaa tgatcggtat ggagaaacga
    29581 aatttattga cacgaaaaag tgatttgcgt aacaccttaa caaaagtagt aaaagaacca
    29641 gcacgttacg atcgacacgc catgaactta ttaaatgaag tatataaaac cctacctgaa
    29701 aaacgttcgg aatggaatta tggagatgaa aatcgaacca agctttcacc atttacaagg
    29761 ggaaaacttg atgagttaac caaatatgtt ttaacaaaag aacctggata tcaagaatat
    29821 atacagattt caaaagcatt gaaagaagaa gccaaaagcc tttatgggga aagcaaaaga
    29881 gaaacaaaga atgcagaaaa aaacgccctc tttgatttaa aaaagcgcac aggaaatgcg
    29941 attttaacag aactgaagag acaggatagt caaataaaac cattaataga aagtgtcaat
    30001 cagatgcaat ctatttacaa tgaacgatcc tttcgaacac tcaaacagtt gccaacaaat
    30061 gaatacaagt atagttatca agaattcgta aagcgtagga aacaattcca acaaagagtt
    30121 gtcacgcagc gaagcttgcg tcaattaaat cgaacaatta atgcacatga agaaaaatac
    30181 cacgcaagta aagcatatga agagcaacag cgaagaatcc aaatggaaca agaaagacaa
    30241 taggaggaag agaaatgaaa caatatttag atcaatggaa agtcatagaa ggatcactaa
    30301 aagaagaacg aatcgaacaa cttcctgatt gtttagaaaa agagcatttg tttcagataa
    30361 gcgaaatgtt gagaaacgaa cagtttgatc caaatcaatt tttagttgtt gagtacccag
    30421 ctactggtgt gtattgttgt aatcatgtga agggtgaaaa atattttatt attcaagaat
    30481 acgaaggtaa gttagcgcct tattatacta cttgggaaat aaatgaagag ggaatcaata
    30541 atttcccatg tgaaagcatt gaagagtcta tttctctcac ggaatgttaa ataaatagag
    30601 acgtaaaaga gtcctgaacg tgattgtcca ggactctttt tcaatgaatt gttttccgcc
    30661 aattcactga gtggtgcgat tcaattttat tatatcaaaa gaaagtgaga ttccaaagtg
    30721 aagggaggaa gaagaaatga ctcagacaac gattggtgtt gtggttgaaa aaagcaggga
    30781 taatctcgtt tttgtgacag aaattcaaac aggtagagct tttgtagtca cagacaaagc
    30841 tgcaaaagcg tatcaaaatg gcaatatttt aacgttaaat ctgactacga agatgttcgt
    30901 tgatgcagta gaaaattatc catttgttta gacaaacgat attttacaag gaggaaacgg
    30961 acatggcaga agaaaaagaa ccactagata atagtcggtt gggaaagtcc aaaagaaaat
    31021 tagtacggtt gcaaaacgaa ttaaataatc aatttcaaaa gctatttgat caccaaaaat
    31081 tgacaaatgg gcaaccaatg aatgacaaac gaaatggtcg ttcatggttt aaccagcaag
    31141 aaaaaataga aaataaaata caaagtttga gagaagagat caagcaacaa gaaaaacagg
    31201 tagaaaaact tgaaagacaa gaagagctaa aggaaatggg ctataacaag tacggcggac
    31261 tggatatgac gatcgataat attccaagaa tcaaagaaga aatcgaacgc tttgaaaaag
    31321 gtgaatcgac tttttcagcc gccacgatcc gaaaatatca acggaagttg gaaacattag
    31381 aacagctgaa agaaaaatca gaaaaaggaa aagaaaatat cctccctgaa gtacaagcga
    31441 tcattgacag cggacgagtg acgcaatgga aaaaaaatcc aaccatttat tttttgaaag
    31501 gctatcgaaa agttgcttta gaactgaatg aaaacggggg atttgaggaa tcaaaaaaat
    31561 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgcac
    31621 caactgattc tctgaaggag aaagaaaaag atcatttcta tcgagtagaa ttcaacgaaa
    31681 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacatta gaattgatcg
    31741 atcaacttaa aacgttggat aaccaatacg ctgcagaagc aggttattat aaattctatt
    31801 ttgaagaagt gaagaacgga gaagtagtag atcagatccg aatggatcta ggggatggcg
    31861 tcaacagtaa tcaccccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
    31921 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
    31981 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatc aaacaagaag
    32041 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatacccat tcttctgaag
    32101 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
    32161 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
    32221 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
    32281 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aacagaagct acaccagaag
    32341 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaa
//
LOCUS       DAYYNG010000016        29831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000016 DAYYNG010000000
VERSION     DAYYNG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.016"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            529..843
                     /locus_tag="ACHBFA_000604"
     CDS             529..843
                     /locus_tag="ACHBFA_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394123.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(896..1669)
                     /locus_tag="ACHBFA_000605"
     CDS             complement(896..1669)
                     /locus_tag="ACHBFA_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394124.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            1774..2019
                     /locus_tag="ACHBFA_000606"
     CDS             1774..2019
                     /locus_tag="ACHBFA_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9394125.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2175..3026
                     /locus_tag="ACHBFA_000607"
     CDS             2175..3026
                     /locus_tag="ACHBFA_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM9394126.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            3059..3736
                     /locus_tag="ACHBFA_000608"
     CDS             3059..3736
                     /locus_tag="ACHBFA_000608"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM9394127.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            4224..5189
                     /locus_tag="ACHBFA_000609"
     CDS             4224..5189
                     /locus_tag="ACHBFA_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM9394128.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5211..5423
                     /locus_tag="ACHBFA_000610"
     CDS             5211..5423
                     /locus_tag="ACHBFA_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9394129.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5498..5974
                     /locus_tag="ACHBFA_000611"
     CDS             5498..5974
                     /locus_tag="ACHBFA_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM9394130.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5971..7779
                     /locus_tag="ACHBFA_000612"
     CDS             5971..7779
                     /locus_tag="ACHBFA_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9394131.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7856..8071
                     /locus_tag="ACHBFA_000613"
     CDS             7856..8071
                     /locus_tag="ACHBFA_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM9394132.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8163..9026
                     /locus_tag="ACHBFA_000614"
     CDS             8163..9026
                     /locus_tag="ACHBFA_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM9394133.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9063..9599
                     /locus_tag="ACHBFA_000615"
     CDS             9063..9599
                     /locus_tag="ACHBFA_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM9394134.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            9613..10035
                     /locus_tag="ACHBFA_000616"
     CDS             9613..10035
                     /locus_tag="ACHBFA_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM9394135.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9965..12364
                     /locus_tag="ACHBFA_000617"
     CDS             9965..12364
                     /locus_tag="ACHBFA_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM9394136.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12396..14387
                     /locus_tag="ACHBFA_000618"
     CDS             12396..14387
                     /locus_tag="ACHBFA_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM9394137.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14410..14661
                     /locus_tag="ACHBFA_000619"
     CDS             14410..14661
                     /locus_tag="ACHBFA_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM9394138.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            14651..15880
                     /locus_tag="ACHBFA_000620"
     CDS             14651..15880
                     /locus_tag="ACHBFA_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM9394139.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15877..17958
                     /locus_tag="ACHBFA_000621"
     CDS             15877..17958
                     /locus_tag="ACHBFA_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM9394140.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18107..22027
                     /locus_tag="ACHBFA_000622"
     CDS             18107..22027
                     /locus_tag="ACHBFA_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM9394141.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22028..22972
                     /locus_tag="ACHBFA_000623"
     CDS             22028..22972
                     /locus_tag="ACHBFA_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394142.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22976..23179
                     /locus_tag="ACHBFA_000624"
     CDS             22976..23179
                     /locus_tag="ACHBFA_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM9394143.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23479..23715)
                     /locus_tag="ACHBFA_000625"
     CDS             complement(23479..23715)
                     /locus_tag="ACHBFA_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394144.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23898..24269)
                     /locus_tag="ACHBFA_000626"
     CDS             complement(23898..24269)
                     /locus_tag="ACHBFA_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM9394145.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(24266..25594)
                     /locus_tag="ACHBFA_000627"
     CDS             complement(24266..25594)
                     /locus_tag="ACHBFA_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9394146.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(25555..25884)
                     /gene="mobC"
                     /locus_tag="ACHBFA_000628"
     CDS             complement(25555..25884)
                     /gene="mobC"
                     /locus_tag="ACHBFA_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9394147.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26142..26512)
                     /locus_tag="ACHBFA_000629"
                     /pseudo
     CDS             complement(26142..26512)
                     /locus_tag="ACHBFA_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            26657..26842
                     /locus_tag="ACHBFA_000630"
     CDS             26657..26842
                     /locus_tag="ACHBFA_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9394148.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27000..27167
                     /locus_tag="ACHBFA_000631"
     CDS             27000..27167
                     /locus_tag="ACHBFA_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394149.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            27785..28444
                     /gene="vanR-B"
                     /locus_tag="ACHBFA_000632"
     CDS             27785..28444
                     /gene="vanR-B"
                     /locus_tag="ACHBFA_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9394150.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            28447..29808
                     /gene="vanS-B"
                     /locus_tag="ACHBFA_000633"
     CDS             28447..29808
                     /gene="vanS-B"
                     /locus_tag="ACHBFA_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9394151.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         7820 a         7450 c         7790 g         6771 t
ORIGIN      
        1 tttctttttt gtttcatatg tcaaatgcca ttttgaaatg taccatctta agaaatttat
       61 ccttaaaaac aattgcgtta ttggataaaa agatgctaca ttatcttaaa aaaggaatca
      121 aatatgatga atttagtggc tagcatatcg tgtgattaag atactttata gtgtaagttg
      181 aaggagaaaa aatatttaaa atatacttcc catttcagtg ggaagtaaaa ttaatcttgt
      241 tgtttctact agtaatgtat ctttttggaa actatgtaac gatgtaaaca agattttttt
      301 cagaagaaaa aacgataaaa gttgtgataa acgatttaaa aaataggaga aaatatatca
      361 agcgcatggt atgaaacgca catttaaaaa gattcgacta actaaaaaaa taagtttaca
      421 aagcattgtt gtgtattaca agtgatctat acactgaaaa atgatagaga aaatctgata
      481 cgtccgcaac aatggacaat tgaaaatgaa agtgaggaaa ttggaagaat ggctgatgaa
      541 aataaagttg ttccgcttga aacactagtg ataaataagg aagagtttga gcaacttttt
      601 cttgattttt ttgttcaata tgacaggatt tatgatttta gttcgtcact agatttattt
      661 gaattagagg cagaaataat agaaaacagt cgagaggaga atggtaaaat catcatttca
      721 gtagaaaaat tcaatgaatt attctcagaa acatttgaaa tagatacaga ttattatcaa
      781 ccacaagaag ttttccaatc atttagaaat cagattattc gctcaggtaa gaccgtaaac
      841 tgaaaaacaa taaaagaaac gttgaaagaa taattattcc ttcaacgttt cttttttaga
      901 atgttttaat gaccaaagca gtttcattgt caggcttagg taattcatga aatcccgtat
      961 ttatagtgct caattgatca actaaccatg ttttactttt tgaattgtag ataaatctaa
     1021 aattaatatc gttattatcg tgttgaaggg gcgtctccaa ataatctgct gtatattcat
     1081 agtcaacgta acctgctaat acaaaagaga gtatcccttt atttaatgtt agatttaatg
     1141 gataatcata cgactttatt tttttcaact tcatattgta catatcttca gccataaaag
     1201 ttgagttcgt gatttcatct tgggtctcat taaaaaagtt atccgaataa tcgcttattt
     1261 ttgaaaagtc tttattggca ccagcctcaa aaaattgttc aactttttca gtataaagtt
     1321 gtgctaacgc tcctttttca atattaactt taatattttc atgtctatca atttgtgtgc
     1381 tgttcagtaa gggaagatca gcttttagtt ttatatcgtc ttgatttctt tcaatttcaa
     1441 ttggcaaaat cttttcagta gattcagttt ctgataaagg aacatctagt tttttatcat
     1501 gataataagc ttcaaatcca tctggaaccg gaaaaaaatg tattgggtaa ttcttttctg
     1561 aggttgttgt actagtttcc gtacttgtag aacttgtgtc agaagtggta cacccgctta
     1621 tagttccgat gagtaaagcg caagttagaa tgattgcaaa tttcttcaaa aaaattcctc
     1681 cttaaaattg aatatggact aatactagtc ccattgtagc ataccgaatc tttaagatta
     1741 ataagaaaaa ggactaattt tagtccaaaa ggtgtgctat tcttgaaaga ggaaaaaatt
     1801 attgataaag aaaatgtaat cggtagcaat attagacgag tccgattaga aaaaggaatt
     1861 ggtcaaaccg agctgattag agacctacag cttcgtaaaa ttgctataac tagggaaacg
     1921 ttggttaaga ttgaaggtgg ccgccaacac attaagttgg atcaattgaa agccatcaaa
     1981 gatattctac aggtgtctta tgaagatttg cttcaatgac tacttgtagt catttcgttt
     2041 gaaattatta tgttacaccc ttatgtgtca cgaagaaata ctggtataat aatgaatggg
     2101 atatgtaacg atagggtata ctgttatgtt gcctaataaa ttgtggatca aaatacaaga
     2161 atggagggat atccttgtat aaggcatatg aattggattt tacaaatgct gacttaaatg
     2221 cattctcctc aagctcgcaa tcatatacac atgtagtaaa tcaaatagat cacaaccaaa
     2281 aatctattaa caagagaata gaaaatctat ttgaaagtga aaatgattta gttgattatt
     2341 attctaagga cagcaaaatt ctagatgcag acgaaattat taaagattgg tttccaacta
     2401 taaaagcaga tatttttata tctcactctc atgctgatga aaaattagct atacgttttg
     2461 catcatggct atttgaaaat tttggtctta ctgcatttat tgattcttcg gtttggggat
     2521 acagttctga tttattaaaa aaaatagacc aaaaatattg ctataaggaa caaacaaaaa
     2581 catatgatta tgataaaaga aatgtaacaa ctagtcatgt tcatatgatg ctttctacag
     2641 ctttaaataa tatgattgat agtacagaat gtctgttttt tcttaataca cctaattcaa
     2701 ttagtttgag caatgaaatc actaatgaac agaaatttac gtattcgcca tggctatata
     2761 gcgaactgac gacagcttca atagtggaaa aaaagaatcc acgattggaa agtaatcctc
     2821 aaatgagcac cgaggatgtt agaagcatta taaagcacta ttcttcgcaa gaaaatctgc
     2881 aaataaaata ttctttaaat ttaagtgaat taacttattt acgaaaagct gatttgaaaa
     2941 aatgggcttt agatgctgtg gaatgcacta aaggaagtaa tacattagat attttatata
     3001 aaaacaccat tttcactaat ctctaatcaa tttactgaat cgaaaggaac cgggtagaat
     3061 ggataagaaa gttttaataa agaggttctc tgaagctatt gaaaaaggaa atgcagcaat
     3121 atttgcaggt gcaggattat cgatgtcaca aggatatgta agttggccgg aactacttaa
     3181 tgatcctgca acagaaatcg ggttggattc taaaaaagaa acagacttag ttactctggc
     3241 tcaatattat aaaaatgaaa atggtggatc aagaggaatt ttgaatcaga tacttatgga
     3301 taattttggt gaagaattag aaatatctga aaatcatcga atactagcaa gtttacctat
     3361 tgaaacttat tggacaacta attatgatca tctaatagaa aaatcaatcc gagaagctta
     3421 taaaaaccct caggttaaga aaaattatac tcagttagca acaactaatc caaatgtgga
     3481 tacaattgtt tataaaatgc atggtgatat agatgatgtc agttcgacag taataacaag
     3541 agatgattat gaaaaatatg atgattcgta cgcgttattt aaagaaactt taaaaggaga
     3601 tttgttaaca aaaacttttc tctttttagg atttagtttt acagacccta atttagaaag
     3661 aattttatca gatataagat gggttttgag agaaaaccaa agacctcatt attgtataat
     3721 gagaaaaata ttatagaaaa aattttaggt tatcgctgaa agccccggaa atacgggcat
     3781 ttcgcaggat atgagaactc aatttactac caatttacta ctttcggatt gagccgcata
     3841 tatgctgaac catactgcta atacccgcca ccccaaatac ggcacatcat aaattgaata
     3901 cgacaccgta aacgcggcgt tcctctttcc cgtcatggga gaacaggacg ccgctttttt
     3961 atgcccaaat agagaaagga ggcgacgcca tgctatttac ttccggccct gaccgggcgt
     4021 ttgaacggct gatgcaggaa aagcccggtt tcgatcacta cgacggcggc ggggcggacg
     4081 cgccggagga ttgcggcacc tgccgatttt accgcccgga ttggaaatat caattctgcc
     4141 agtttgcaga gtgtccctat cagccgggca agctgaccga ctatgcagcg gtcaaattca
     4201 cagtgaaagg agcgaacaac gctatggcag ttttccgtgt ggaaaagaac aaaggctata
     4261 cggtgatgtc aaatcaccac ctgcggaata agaacctgac cttaaaggcc aagggcctgc
     4321 tgtcgcagat gttgtccttg ccggaggatt gggactatac cctaaaagga ctgtcctata
     4381 tcaaccggga aagcatcgac gcgatcagga cagcggtatg ggagcttgaa aaagctggat
     4441 atatccggcg tgagcagggc cgtgacgcaa aaggcaaaat ggccgatatg gtctatacca
     4501 tctacgaaca gccggtattg gaaaacccgg tattggaaaa tccaacatcg gataacccgg
     4561 tgttggaaaa tccgacatcg gataatccgg cgtcggaaaa tccaacgcaa ttaaataaag
     4621 aagtacaaag aactgactta tcaaaaaaag aaaaaataat tacagatgga caaagtaccc
     4681 attccattcc tatcccatcc ccgacccctg cccctatgga gggcgaggca gcggctacgc
     4741 cgccggaacg gaaaaggaca ggaaaggatg aggctgtacg gatttatcgg gaaatcattt
     4801 tggagaacat cgagtatgcc tatctcattc aggacaggag cattgaccgt gaacagcttg
     4861 acgaaatcgt agacctgatg ctggaaaccg tctgcaccgc ccgaaagaca atccgtgtcg
     4921 ccggggacga ctaccctgcc gagctggtga aatccaagta catgaagctg accggcgaac
     4981 atatccgctt tgtccttgac tgtctgcggg agaacacaac gaaagtccgc aatatcaaac
     5041 aatacctgcg ggctatgctg ttcaacgccc ccagcaccat ttccaactac tacacggccc
     5101 ttgtcgcgca cgacatggca cagcccgatt ggggaaagcc taaatccggc ctgccggact
     5161 attcctgcgg cgagggcgaa agcctgtaac gagaaaagga ggacgaccct atgaagcagg
     5221 gcgcattgat ttttgacgag cgaacagacc gttacgacat ccgctttgat ctggccgact
     5281 attacggcgg cctgcattgc ggggagtgtt tcgacgtgat gatcggcggc aaatggaggc
     5341 ccacccgcat tgaaatggcc gaaaactggt atcttgtcgg catccgggcc gacgaccttt
     5401 ccggcctgcg ggtgcggatt tgacctatat ctacctgcgg cgtctggtgg ttccgcctta
     5461 gacgccgctt ttgactttct gacaaggagg gatgcgattg caggaagaag ttactcaaaa
     5521 gaccattgcc ctgtccatga aaacgggcaa gctcacggct caagtgctgc aagcggcgct
     5581 gaaaaagtac ctgcaacacc gggccaaggg caaaaacacg ctgcaccacg gccagcagag
     5641 cctaaagcag cttaaaaagc atggggctga tctctccaac attgagatca ccgaggccaa
     5701 cattggagcg ttcaagccat gcgccaagaa atacggcgtg gattttacct tacgaaaaga
     5761 taagaccacc cagccgcccc actacgttgt gattttcaag gccaaggatg cagataacct
     5821 tgatctggcg tttcgggagt ttaccgccaa gacgctttcc aaggagcagc ggccctctat
     5881 ccgcaaggtt ttgtccgctg tgaagcaaaa ggcggcggcc cagaccaagc agcgggccaa
     5941 ggagaaaatc aaggaaaggg ggctggaact gtgaagcctg acgtaaagaa attgctgatc
     6001 ctgaacctcc cgtatctgat ttttgtgtac ctgtttgcga aatgcgggga ggcgtaccgg
     6061 ctggccgctg gtgctgacct gtcggggaag ctcctgcatt ttgcggatgg ttttgcggcg
     6121 gcttttgcca accctctgcc aagcctgcac ctgtttgacc tgtgtatcgg tatcgccggt
     6181 gccgtgatcg tgcggctggt ggtgtactgc aagagcaaga acgtgaagaa ataccgcaaa
     6241 ggcatggagt atggcagcgc ccgctggggg acggccaaag acatagcgcc ttacatcgac
     6301 cccaagtttg aaaataatat cctgctcacc caaacggaac ggcttaccat gaccggcagg
     6361 cccaaagacc ccaagacagc gcggaataaa aacgtgctgg tgatcggtgg cagcggttca
     6421 gggaaaacga gatttttcgt gaaacccaac ctgatgcaat gctttccgac cacggactat
     6481 ccgacttcgt ttgtggtgac agatccgaaa ggcactttgg ttctggaaac cggcaggatg
     6541 ttccagcagg cgggctaccg cattaaaatc ctgaatacca ttaacttttc caagtccatg
     6601 aagtacaacc cctttgtcta catccattcg gaaaaggacg tgctaaagct ggtaaatacc
     6661 ctgatttcca acaccaaggg cgagggggaa aagtcagcgg aggatttttg ggtgaagtcg
     6721 gaacggttgt tttattcggc cctgatcggc tatatctggt acgaggcccc agcggaggaa
     6781 atgaacttct caacgctgct ggaaatgata aacgccagtg aagcccgcga ggacgacccg
     6841 gagtttcaaa gccccgtcga tctcatgttt gagcgtttgg agcagaaaga ccccgaccat
     6901 tttgcggtgc gccagtacaa gaaattcctg ctgtccgcag gcaagacccg ttcttccatc
     6961 ctgatttcat gcggtgccag acttgcgccc tttgacatcc gggaggtacg cgagctgatg
     7021 gaggacgacg aaatggagct ggacaccatc ggggacgaaa agacggtgct gtatctcatt
     7081 atgagcgata cggacacgac ctttaatttc attctggcta tgctccaaag ccagctaatc
     7141 aacctgcttt gtgaccgggc tgatgatgtg tacggcgggc ggctgcctgt ccatgtacgc
     7201 ctgattttgg atgaatttgc caacattggc caaataccca atttcgataa actcattgcc
     7261 accattcgaa gccgtgaaat atcggcctct attattttgc agagccagtc gcagctaaaa
     7321 gcgatttaca aggacgccgc cgagatcatt tcggataact gcgatagtgt gcttttcctt
     7381 agtgggcgcg gcaaaaatgc caaggagatt tccgatgcgc tggggaaaga aacgattgac
     7441 agtttcaaca ccagcgagaa ccggggaagc caaacgtccc acggattgaa ctatcaaaaa
     7501 ttaggaaagg agttgatgtc ccaagacgaa attgccgtga tggacggcgg caagtgtatc
     7561 ttgcagctac ggggtgtgcg cccgttttta tcggagaaat tcgatattac caagcatccc
     7621 cggtacaaat accttgccga cgccgacaag aagaatacct ttgacgtgga cagatttttg
     7681 actatcacgc gccggaaacg gcagcaagtg gtaacgcagg atgaagtatt cgacctgtat
     7741 gaaatcgacc tgtcggacga ggacgccgcc gctgaataaa cagcggcttt tttcattcca
     7801 acaacaaaat caaaaaatca ttatgagccg gaaaccggca gaaagaggta aatttatggc
     7861 attttttaat tccgcagtta ctactttgca gactatcgtg atcggactgg gcggcgctct
     7921 gtgcgtatgg ggcggcatca atctgttgga gggatacggg caggacaatc cgggggcaaa
     7981 atcgcagggt gtcaagcagc ttgtcgctgg cggcggcgtg gccctgatcg gtgtcactct
     8041 ggttcccctg ctgtccgggc tgctgggctg atcctggcct ttgtccctgt ccaaaccctt
     8101 aactaatcgc ctccgcgtcc tcccggccag accgggaggg cgcgggaaag gaggtgctac
     8161 ccgtgattag tgaaatcatt gaagaatgga taaaaggcat cctcatggac ggtatcacgg
     8221 gcaacctgtc cgggctgttc gatacggtaa atgccaaagt cggagaaatc gccgccgacg
     8281 tgggacgaac cccgcaggca tggaacagcg gtattttcaa tatgctgcac agcctgtcgg
     8341 aaaccgtgat cgtccccata gccggggcca tactcgccct tgtcatgtgc tatgagctta
     8401 tccagatgat aacggaaaag aacaatatgc acgactttga cagctccatg ttcttccgct
     8461 ggatattcaa atccgcgttt gcaatcctga tcgtaacgaa cacatggaac atcgtcatgg
     8521 gcgtattcga cgccacccaa agcgtcgtca atcaaagcgc cggtgtgatt atcgggcaga
     8581 catccattga ttttaacacc ctgataccag accttgaaag ccaattggag gctatgagcg
     8641 tgggcgctct tttggggctg tggttccaaa cccttgttgt gggcctcacc atgaatatcc
     8701 tttccatctg cattttcctt gtgacctatg gccgcatggt ggaaatctat gttgtcacgt
     8761 cgctggggcc tatccccctt gccacaatgg gcaacgcgga atggcgcggc atgggccaag
     8821 ggtatctgaa atccctgctg gcgctgggct tccaagcctt tttaatcatg gttgtggtgg
     8881 gtatctacgc ggtgctgata cagaatatcg gttccgcttc cgacatatcc ggcgcaatct
     8941 ggggctgtat gggctatacg gtgctgctgt gcttctgcct gttcaagacc ggcagtatat
     9001 caaaggccgt attcggggcg cattgatccc aaaatccaag catacacagg aggaacttgt
     9061 ttatttcaac gtacaacatc atttacgctg acccgccgtg gcggtatgac tgcaaaaacg
     9121 tacagggcgc agcggaaaat cactattcca ccatgagcat tgacgagctg tgcgccctgc
     9181 cggtggaacg gctggcgtcc aaagactgcc tgctgttttt gtgggccaca ttccccatgc
     9241 tgccggaagc tctgcggtta atcaaagcgt ggggcttttc attcaagacc gtggcttttg
     9301 tatggctgaa acagaaccgt aaaagcctta catggtttta cgggctgggc cgctggacaa
     9361 ggggaaacgc ggaaatctgc ctgctggcaa cacacggcaa gccgaagcgt cgttctgcct
     9421 ctgtccatca gtttattatc agcccgattg aacaacacag caaaaagccg gatgtgaccc
     9481 gtgaaaaaat cgtagagctg gcgggcgatt tgccccgcgt ggagctgttt gccagacaga
     9541 aaacccccgg ctgggacgtg tgggggaacg aggtggaatg tgaccttatc ctgacgtgaa
     9601 aggaggtgcc aaatggctta tgtaaccgtc cccaaggact taacccatgt gaaaagcaag
     9661 gttgtgtttg ggcttaccaa acggcaactg atctgttttt ccggggcgct ggtgatcggc
     9721 gtccccttat attttctcac ccgtgactat cttaccaaca gcgcggcggc cctgctcatg
     9781 gtatttgcca tgctgccggg ctttttgctt gccatgtacg agcgtcacgg ccagcccctt
     9841 gaagtggtga ttatgcagat catccagtgc tgcttcatcc agccaaagga gcgtccctat
     9901 cagaccgaca acacctacac cgcccttgtg cggcaatacc agatggaaca ggaggtaaag
     9961 gccattgtcc aaaaaggaaa tcccaagaga aaccgaaaaa acaaagctga cccgcgccca
    10021 gcgaaaagaa attgacgcgg taatccgtaa atataagggc gacggcaggc cccacacggc
    10081 gcagcagagc atcccctacg aggtcatgta cccggacggc gtgtgccggg tatcgcccgg
    10141 cgtcttttcc aagtgcattg agtttgcgga catcagctat cagcttgccc agccggacac
    10201 gcagacggct atctttgaaa aactgtgcga cctgtataac tatgtggacg cctccattca
    10261 tatccagttt tctttcctca atcggaaagt tgacccggtg cagtacgcca agagctttga
    10321 gatcgcgccg cagggggacg acttcgacga catccgggcc gagtacaccg gcattctgca
    10381 aaagcagctt gcaaacggca acaacggcat ggtaaagacc aaatacctga cattcaccat
    10441 cgaggccgaa agcgtgaaag cggcgcgagc cagattgaag cgcatcggct ttgacctgtt
    10501 aggctatttc aagagcatgg gggccgtagc ccatgtgatg gacggctggg aacggctgaa
    10561 cctgctgcat ggcgtgtacc acccggacgg cgagatattc aattttgact ggaagtggct
    10621 ggccccgtcc ggcctctcca ccaaggattt tattgccccg tcctccctgt gcttcggcaa
    10681 tgcaaagaca ttcggcatgg gcggcaaata cggcgcggta agtttcctgc aaatcctgtc
    10741 cccggagctg tcggacgata tgctggccga cttcctcaat acggaaagcg gtgttttagt
    10801 caatctccat gtgcaggcca ttgaacagac caaggcgatc aagaccatca agcgcaaaat
    10861 taccgacctt gacgctatga agattgcgga gcagaaaaag gccgtccgca gcggttacga
    10921 tatggacatt cttcccagcg accttgccac ctacggcgag gacgccaaga agctgctgac
    10981 aaagttgcag acccgcaatg aacggctgtt ccagttgacg ttccttgtcc tcaacgtggc
    11041 cgacaccaag caaaagctga ataacgacgt gttccaagcc gcaggcgtgg cccaaaagca
    11101 taactgtccc cttgtccggc tggactacca gcaggaacag ggccttgcaa gcagcctgcc
    11161 gctgggcgtc aatcagatca agatacagcg cagccttacg acttccagcg tggccgtgtt
    11221 cgtccccttt gtgacgcagg agctttttca gggcggcgcg gccatgtatt acggcatcaa
    11281 cgccaagtcc cgcaacatga ttatgctgga caggaagcag gcccgttgcc ccaatgcctt
    11341 gaagctcggc acccccggca gcggcaaatc catgagctgt aaatccgaga tcgtcagtgt
    11401 gttcttaacg acgcctgacg atatttttat ttcagacccg gaggccgaat attaccccct
    11461 tgtcaaacgg ctccacggac aggtaatccg gctgtcgcct accagcaagg actttgtgaa
    11521 cccgctggac atcaacctga attattcgga ggacgataac ccgctggccc tgaaatccga
    11581 ctttgtgctg tcgttctgtg agcttgtcat gggcggcaag aacgggctgg aagccattga
    11641 aaagaccgtg atagaccggg ctgtgcgcgt gatctaccgg ccctatctgg cagacccccg
    11701 cccggagaat atgccgatcc tgtccgacct ccacaaagcg ttgttagatc agcatgtccc
    11761 ggaggctgac cgggtggcgc aggcccttga cctgtatgtg tccggctccc tgaacgtatt
    11821 taaccaccgt accaacgtgg acatcggcaa ccggcttgtg tcctttgaca ttaaggagct
    11881 gggcaagcag ctcaaaaagc tggggatgct cattgtccaa gaccagatat gggggcgcgt
    11941 cacggccaac cgaagccaag gaaaggccac atggtatttt gccgacgagt tccatttgct
    12001 tttgaaagag gaacagacgg cggcctattc tgcggagatt tggaagcggt ttaggaaatg
    12061 gggcggcatt ccaacaggtg ccacccagaa cgtcaaagac ctgctttcct ccccggaaat
    12121 tgaaaatatc cttgaaaaca gcgacttcat tacgctgctc aatcaggcgt ccggggatag
    12181 aaaaatcctt gcggaacgct tgaatttgtc caccgaacag cagaagtaca tcgacaattc
    12241 cgaacccggc gagggccttt tgatctttga aaacgtggtg ctgcccttta ccaaccccat
    12301 cccgcacaac acccagcttt acaagattat gacgacccgc ttaaacgagg tggcgggagt
    12361 atgacccggc gacgccacaa aggaggtgaa ccaccatgaa agagctgaaa gccaaggcga
    12421 aagtgacgca ggccatgacc cgcgacgggc tgacgctgga aaatcaggcc accggcgagg
    12481 tggagaacat ttccagccgg gaggccgaac aggatttgtc cgcgccgggg ccgggcggca
    12541 ccgccgataa gctgctggaa cgggccgacg ccatccatga cggccataag gccaagaaag
    12601 ccgcgaagca ggccggggct gtggccgccg agggggacgc tgccctgcac cggccatccg
    12661 cccggctcca actgtcggag gaagaacggg ccgacccgga tttacagccc tatatccgaa
    12721 aatcggacaa acgggccgat aagctggata aggcccgcgc cgccatcccc aagaagcgcg
    12781 tcccggtcaa ggaaaccgtc tttgacgagg caagcggcaa agcaaagacc gtcctgcact
    12841 ttgacaaaca ggacaagggg cctccctccc tgaaacccaa tccggcaggc cgtcctgttt
    12901 ccgaggtttt tctattcggc cacgggaaaa tccatgaagt cgagcatgaa aacgtgggtg
    12961 tggagggcgg ccataagggg gaggaacttg ccgagcggca ggccggaaaa gccatccgca
    13021 gcaccatccg gcaccacagg ctgaaaccct actgggacgc ggaaaaggcc gagcgcaggc
    13081 tattgtctgc caacgcggag tatttctacc agaaatcttt gcgggataac ccggagatcg
    13141 cacaggcggc cagcaacccc atttcccggt tctggcagaa acagcagatc aagcgccagt
    13201 acgccaaggc cgcccggaca gcgggccaga ccgcaggcgc ggcggcacag ggcgcggcca
    13261 agacagccgg ggcctccacg aaagcggcca aggccgcagc caaagaaagt gaacgggcgg
    13321 cctcctttgt ggcccgccac tggaaagggg cgctggccgt ccttgtgttg gggcttttga
    13381 tgctgcttat catcggcggc ttacagtcct gcaccgccat gttcggcagc gccggaagcg
    13441 gcatcacggc gtcctcttac ttttccgagg atagcgatat gttggccgcc gaggacgcct
    13501 acgcggagct ggaagccgag ctgcaagact atctggacaa ctacgaggcc acccatgact
    13561 atgacgaata ccattttgac cttgacgaga tcagccatga cccctatgtg ctgatttcca
    13621 ttctgtcggc gctccatgac gggatattta ccatcgggga ggtacagggt gatttgtcca
    13681 tgctctttga caagcaatat atcctgacag aaaacgtcgt ggtagaaacc cgttaccgca
    13741 ccgaaaccga tacatggacg gacgcggagg gcaacaccca tacagacacc taccaagtgc
    13801 cgtatgacta ttacatctgc tacgtcacgc tggaaaactt cgacctgtcc catgtgccag
    13861 tctatatcat ggacgaggaa acgctaagtt tctattccct gtatatgtcc acgctaggaa
    13921 accggcctga tctgttttcc ggcagccagt accccaacgc ctccaccctg acgccgccga
    13981 cctactacga cgtacccccg gaggctttgg aggatgccac ctttgccgcc atgctcaaag
    14041 aggcggaaaa atatatcggt tatccctatg tgtggggcgg cagctctccc agcaccagct
    14101 ttgattgcag cggcttcatt tcgtgggtaa tcaatcatag tggatggaac gtgggacggc
    14161 tgggggcgca gggcctatat aacatctgca cccctgtttc cagttcgcag gccaagcccg
    14221 gcgaccttgt atttttcaag ggaacctacg acacccccgg cgtgtcccac gtcggcctgt
    14281 atgtgggcaa caatgtgatg ctgcattgcg ggaacccgat ttcctacacg aaccttactt
    14341 caaactactg gcaatcccat ttttacagtt acgggcgttt accttaacgc cagaaaggag
    14401 ctttcaatta tggcaaccaa acttgaccgc atcgaaaagg acatccagaa aaccaagtct
    14461 aagatcgcgg agtttcagaa acagcttcgg gagctggaaa cgcagaaaac cgaacaggag
    14521 aatttgcaga tcatccagct tgtgcgcggc atgaacatga agccggagga atttgccgcg
    14581 ttcttacgca gcggcgcaat gcaggccgcc cctgccgcaa ccccttacca tgaacaggag
    14641 gacaatgccc atgaagaata agaccagacg gatttcccgt attctccccg cacttctggc
    14701 cgtgatgctc tgcgtcacgg cctttcccgt taccgccctt gcaggcggca atgacccggc
    14761 accggccccg ctgccggagg ctacaaccga acccgctacg ggcggtttgg agcctgaaac
    14821 agacgaaacc ggcaccgcca cggacgggac agaaaccgag gccgaaaaag ataccggccc
    14881 tctgaccggc aaggacatca ccgacctgtt ttccgcgctg ttcggttcca aggtgagcat
    14941 tgccgccacg gacgacggca tccagatcac caccggcacc gacaaaaaag aacctgaaca
    15001 gaccggcacc gtcaccacca acggcggcag gctgaatgtc cgcaccggcg cggggcttga
    15061 caagaccgcg ttcacccagc ttcccaatgg gacgacggtt gaggtagtcg gcacagacgg
    15121 cgactggatt aaaattctct tgccggagcg catcggctat gtccattccg actatatgac
    15181 cgtcagcgaa aaggaagtag cggctaccgg cgagggcggt ttttccctct ccattgaccc
    15241 ggaggaaatt gcttctctgc tggaactgtt caatggcggc ggggccagcg ccgccctgtc
    15301 cccggacggg aacctgtcac tgattgacga catcggcagc gccgcgaaaa atggcaagca
    15361 gtttatcacc gtggaaacca aaaacggcaa tgtgttttat ctgattattg accgggacga
    15421 cgagggaaaa gaaactgtcc atttcttaaa tcaggtggat gaagccgatc tgctgacgct
    15481 gatggaggac ggggaaaccg tcacccccgc tgctgtctgc tcctgtacca cgaaatgcaa
    15541 ggccggggct gtgaacacga attgcccggt atgtaaaacc aacctgaccg agtgcagcgg
    15601 cccggagccg caggaacccc agccggagga acccgaagcc ccggaggaag aacccaaggg
    15661 cggcgcgggc ggcctgatcg tgttcctgct ggtggcgctg gcgggcggcg gtgcggccct
    15721 ttactatttc aagttcaaaa agcccaaggc tgaaaccacc ggcagcgacg atttgggcga
    15781 gtatgatttt ggggaggacg aggatttgga ggacgaggaa cccgaaaccg agtttgaccc
    15841 ggaagctgat tttgtgagtg aacaggagga cgaggaatga catataaact tgtaatcgcg
    15901 gaaaaaccca gcgtggcccg cagcattgcg ggcgtgatcg gggccacgga gaaacacgac
    15961 ggctatttgg aggggaacgg ctatctggtg agctggtgca tcggacatct ggtgtccttt
    16021 gccgacgccg gacgctatga cgagcgtttc aaaaaatggc gctatgagga tttgcccatt
    16081 ctcccggaaa catggcagta catcgtcccc gacgacaaga aacagcagtt tgacgttctc
    16141 tgttcgctga tgcagcgccc cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc
    16201 gagggggaat tgattttccg tttcgtctac cagatggcgg gatgccaaaa gccctttgag
    16261 cgcctttgga tttcctctat ggaggacagt gctattaagg acggctttgc ccacctgaaa
    16321 ccgggcggcg actatgatcc gctgtatcaa tcggccctgt gccgggccaa ggccgactgg
    16381 ctgatcggca ttaacgccac ccggcttttt tccgtcctgt accacaagaa cctgactgtg
    16441 ggccgcgtcc agacgccgac cttaaaaatg ctggtggaca gggacgccaa gattaccgat
    16501 ttcaagaaag agaaatatca catcgtccat atcacggcgg gcggcgcgga cgcggtaagc
    16561 agccgctttt ccgacgctgc cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag
    16621 gccgtttgcg tttccgtgac gcgggagaaa aagacggaac agccgcccaa gctctatgac
    16681 cttaccacct tgcagcggga ggctaaccgg cttttcggct ataccgccaa gcagaccctt
    16741 gactatgccc aggcactgta tctaaagaag ctgctgacct atccgagaac ggatagtcaa
    16801 tatcttaccg acgatatgct gcccgccgtg gaaagccttg tgtccggcct gtgggagctg
    16861 gtgccgtttg ccaagggcct gaacctttcc ccgcagtttg accgcatcct gaacagcaaa
    16921 aaggtgtccg accaccacgc cattatcccc accgctgaat ttgtgaagca gggctttggc
    16981 actctggccg agagtgaaaa aaagctgctg tccctgatct gctgcaagct cttatgtgcc
    17041 gtggccgggc cgcatatcta tgaggccgtc accgccacat ttacctgtgc cgggcaggag
    17101 tttaccgcca agggcaagac cgtcctttcc cccggctgga aagagattga acgccggttc
    17161 cgttcttcct taaagaccga cgccgacgag gacgccgagg cggtgcggga gctgccggag
    17221 ctttgcgagg gccagacctt tgcagacgtg gccgcctctg tcaccgagca tttcaccacg
    17281 ccgcccaagc cctacaccga agatacgctt ttgtccgcta tggaacgggc cggggccgag
    17341 gatatgccgg aggatgccga gcgcaaggga ttaggcacac cggccacccg cgccgccatt
    17401 ctggaaaagc tggtgcagat gggctttgtc cagagaaaag gcaagcagct cattcccacc
    17461 aaggacggca tcaatctggc tgtggtgctg ccggaggcgc ttacttctcc ccagcttact
    17521 gccgaatggg aaaaccggct gaccgagatc gccaaggggc aggccgatcc tgacgaattt
    17581 atggacggca tcgagacgca ggcgcgggag ctggtgcaga cctattcctg tatcagtgag
    17641 gaaaagcaga aattgtttca ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt
    17701 gaggctgtct atgagggcaa gaaaaactac tactgcggaa accgggcctg ccagttcgtc
    17761 atgtggaaga atgaccgatt ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc
    17821 gctgcgctgc tcaaaaccgg caaagtcaag gtaaagggcc tctattccgt aaagaccggc
    17881 aaaacctacg acgggacggt gcttttggct gataccgggg gcaagtacgt caactaccgc
    17941 attgaacaca ggagctaaag gacagtaaca cagcaagcat agggagtgtg gctacactcc
    18001 cggtaaaatc ccccggccgc gtccctgccg ggccggggat ttttgcttgt tggggaagtc
    18061 cccaaccccc tattttttga aacagaacag agaaaggagt gaccgcgtga acgaagtatt
    18121 ttctatccaa gtccataacc ggcagttttt ggaacagggc aaacaaggcg tctggctgga
    18181 tttgcccacc acaaccgaaa cgctgcaagc cgccctgcgg gatgttggca ttactgccga
    18241 caatccgcag gattttttta tcaacgggta ttcctgcccg gaggacagac accttgccat
    18301 tccctatgac atggtgctgg ccgccgacgt ggacgagctg aatttccttg ccgcccggct
    18361 gggccagctt gacgccgccg agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg
    18421 cggctttgag aatatcggcc agatcatcga ctacaccgaa aatgtggatt actatgtgca
    18481 tcttcccgac gttagggggg atggacagtt gggcgactac tacctgaacc gttccggcat
    18541 ggtggatatg ccggaggaat ggaaagccgg gattgatacg gcgcagtttg gacgccacat
    18601 tgcccagcag gaacaaggcg tttttaccca gtacggctat ctggtgagaa gcggcgacga
    18661 atggcagcgc gtccatgagg gccagccggt gccggaggaa taccgggtga tgcactaccc
    18721 ccagccggaa gctgaccggg aggaaccgca ggcccagcag ccggaggcat ccgaaagccc
    18781 cgacaaagcg ccgcccccgg ttatcccgat catgctggac ggcatgaaca gcaaagaacg
    18841 catgaaagtg attactgacc gtctggaaac cggcgtacag gagctttttg aaagtagccg
    18901 ctatatggct tacttatccg ctatgtccaa gatgcacgat tacagtctga acaatacgct
    18961 gctgatcgcc atgcagggcg gttcgattgt ggcgggctat aacaggtggc aggatgaatt
    19021 tgaccgccat gtgaagcggg gcgagaaagg cattaagata ctggccccgg ccccgttcaa
    19081 ggttaagcag cagatggaga aaaccgaccc ggccacccag cagcccgtga tcggcgcgga
    19141 cggcaagccc gtgaccgaag aacaggaagt gacggttccg gcctttaagg tggtgacagt
    19201 ctttgacatc agccagaccg agggcaagga ttttcccatg ccctatatca gtgaacttac
    19261 cggcgacgtg cagcagtatc aggatttctt tgccgcgctg gaacggacat caccctatcc
    19321 cataagcatt gagccgattt cagacggggc caagggccgc tgcttctacg caaaacagcg
    19381 cattgccatc aacgagggca tgagcgaatt gcagacccta aagaccgcca tccatgagat
    19441 tgcccatgcc aagctccacg ccattgaccg ggaggccccc gccgaggaac aggcggcccg
    19501 gccagaccgc agcacccgcg aggtacaggc cgaaagcgtg gcctataccg tctgccagta
    19561 ttacgggctg gacacgtcgg actattcttt tggctatgtg gccgattgga gcagcggcag
    19621 ggagttatcc gagctgaaag cctcccttga aaccatccgg gccacggcca gcgaattgat
    19681 aaccgagatc gacggccatt ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca
    19741 ggaggccgtc acggttgacg ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga
    19801 acaggccccg gcccttgatc cggcagccga gccgcttgtg actgtgctat ggagcgaaag
    19861 cccccagcta aaggacggcc agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg
    19921 tctggatgca gcatatccgg cagaggcagg ctatgacaaa accgcgttcc gcattgactg
    19981 tatctttcag ggcgagccgg acagctacga ggggcggcag gattttggag atagggacgg
    20041 ttccctgatc gaacatatcc agagctacca ccaattttac gcgcaggacg aaaactggaa
    20101 aaagcatgta atcgctacaa ccggcataga gggctgggag caggacaagg cccatcggga
    20161 tatgctgtta ggcgagtttg tcccctatat gaagctgcat tgcagccttg cccaaatgga
    20221 acagacggcg cagggccgct tacagtccgg ggaaaccctg acgccggagg aaacggccta
    20281 tttgggggcg cttctctctt atgtgcagga gtgccgcccc atgctcaatc agggccagta
    20341 tcagttgccg gagccgccga aactggctga cttcgaccag agcttgcagg actacaaagc
    20401 acaggtacag gccgagatcg cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata
    20461 cgcggcggct ggctatgaag caccccaaca ggacaccttt tccatctacc agcttcgagg
    20521 cggggaggaa acccgcgact atcggtttga gccatacgag cgtttacagg ccgcagggct
    20581 ggccgtggac agtgccaact atgaccttgt ttatacggca ccgctggata aaagcacgtc
    20641 tttggaggat atttaccgca cattcaacat cgaccacccg gcagatttta ggggacacag
    20701 cctttccgtt tccgacgtgg tggtgctgca ccagaatgga caggacaccg cccactatgt
    20761 agacagcttc ggctatatgg aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc
    20821 cgaactgtct acggagcaga acgaaaacat gattgacggc attatcaaca atacgccgcc
    20881 cgaaccgcag ccggaggaac ccgaacagct atcttttgag gatgtggcag ttgcttccgg
    20941 ccagacacag gaggaacccc cggaggctgc gcccccgcaa acggctgtcc gctattatcc
    21001 catcaatgag ggagccgccc gccgcgccaa ggaagccatg agcttttctg actaccggcc
    21061 cggcagcgcc acggcggaat accggcacta tgtcgatcag gccgtggaaa ttgcggagcg
    21121 ccagaaaaag cgtgtggagc cagagtatca cgaaaagata gacagcctcc ttgacaccta
    21181 cgcccgcaag ctggccgaga acatgaataa gggctatgag attaccgccc gcgtcccctc
    21241 tgtcctgatt gccgggccgt ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc
    21301 cagtgaccgc aacatggagg aatttcagta cattcagggc ttgcttgaca aaatccgcag
    21361 caccggcatg ggtggtatca gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa
    21421 actggaaaag ctggaagcgt cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa
    21481 gcatgggacg ctggacggat gcccccatct gatcgaaaga ggcattgaaa acctgaaagc
    21541 ggatatggcg tcgggctggc actatgagaa aaaaccgttc cagtcctggc agctctccaa
    21601 caataacgcc gagatacgcc ggttaaaggg ccgcattgag gaattgaccc gccaaaagga
    21661 ggcggcctat gtgggatggg agtttgacgg cggcactgtg gagatcaacc gggtggccaa
    21721 ccgcctgcaa atcttctttg aggggaagcc ggatgctgct gtacgcgacg agctgaaaag
    21781 caacggtttc cgctggtcgc ccaaggccgg ggcatggcag cggcagttaa acgacaccac
    21841 aatccgcgtc gctgaccgta tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc
    21901 cttacagctt gcggcccgca gggagaaaga aaagccctct atccgggcgc agctcaacgc
    21961 ggccaagacc gaacaggcca agcagccacc ggcgcgggaa aaatcaaagg gattggaggt
    22021 agagtaaatg cagaagtttg aaaatgtgga cgttctggcc gcgctggaac agatcatgcg
    22081 gcagaacacc gcgttttatc aaagtgactt tggcattgac aaagacatta tcaggcggga
    22141 tgccgccagc gatcaggccg cagacaagac cctgctctgg atgtcccggc cctccgggac
    22201 gtactgtttc cgggaacgtg atgttttcct gaaagacacc cgacagtaca acacatggaa
    22261 attttacggg gagcagaccc gtgacaagat actggcctac gcggtggagc tgaccggcac
    22321 acagggcggc actatccggg gcaatctcta tgagctggac tatccgcagc atttccggca
    22381 cgtcattgaa accgcccagc cggtgagtgt gaaccggctc ttttatgagc atggcacccg
    22441 tgacatcccg gagagccagc gttttgacgg aagccctgac cgggtattgg gaaacttttt
    22501 gtactatgag gcccagcccc atgacccggc tgtgctgcag gaggcgctac ggacggagca
    22561 gcacagccgg gggcgggccg tccccggcga tttcaaagtc cacgtcaccg ctttgcatga
    22621 cagccggatt gaaaccgagg cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc
    22681 taacagcccc aataaaacgc atttcatggt ggagatctcc ccgcactttg cggcgattgc
    22741 ttccaccaag gataatgacc ggctctttgc catgctgccc tataaatccc tgtgttttac
    22801 cgggatgaaa ggcaggcatg gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt
    22861 gagtattcgc aggccccggc cctctatccg caagcagcta tccgacagca aacaggccgc
    22921 cagcccaaaa aaggcggcgg ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat
    22981 gcactttacc gtggaggaag aaaacctgat ctgtatgtac cacaatgcag accggcgcag
    23041 aacgataaac aggctcaccg ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc
    23101 ccagcagacc ctttccaagc tggaacgcat gacggatgcc gactatgacg gccagaagtt
    23161 ccacttcaca gacgaataac gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg
    23221 cgtccggcgt cttttctttt tgcctattcc tacgcattta gaacccggta ttgtcgggga
    23281 taggatgaag tattcgcttg cccctgcaaa catgcgggag aagccttgca gcgggcggtt
    23341 ttcaatccct taaatgcgta ggttgcagct ataattagac ccctgaaaca gatatagccg
    23401 cgcagcgggt gtatctgttt caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg
    23461 gtgcagccct cttgccgtct atctctgttc cagcgggaag ctcggcaatg cttccagcag
    23521 tttcagggaa atgcgctgcc gtatatcttc gtcaatgact ttcttctttt tgccgtttgc
    23581 ctgtctgatc tccaccgtgg aaagctcgtt gatgtagtca gcataatggg ccaacacttt
    23641 ttccgtggcc cacctctcac cggcaacggc ggctttgatc gtttcatagt ccagcagctt
    23701 ttcttgtccg tccatcctga tacccccgtc agactgattt tttctatgat accatattcg
    23761 gaagcatgga accagagaaa acggcgcttt tctgctggta aattctacgc atttagaaca
    23821 gcacttcggc ggggatagga taaaacaccc acataccccg ctgaccatgc cggaaaagcc
    23881 ttgaaatata agggctttta cagctctaag tgcgtaggtt tccagtatct tttccgctgg
    23941 cgctcggcct ctttgcggcg gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg
    24001 ttagacttcg gcgtgaacgt ggccccgcac tcacagcagc gtttcacttc gtcccggtct
    24061 ttggtgatct cggcatacag ggccgcatcc agcgggagga cagcggcctt aaaccagcgg
    24121 cacagaagcg aataggaaat gctctgtacg caaatacatt cttcgccgtc gtccagcaac
    24181 aggcagttgc cgtcacaata gttgcagcac tcatgcgtca gcttccgggc cttgcgatat
    24241 tgagggtaat tcattcgtgg aatactcata gctcttgcgt ccattccttt cccggcgtca
    24301 tacgcaaaat gccgtccaca ttctgcttga tgatgccgta ttcctgcatc tgctttttcg
    24361 cttcgccgta cttctcatac agccgttcct tttcctcggc caatcgcttg tactcggctt
    24421 tcagggcgta cagatcgggg agctttttca caccctgtcc ctttagggcc tttgccgcag
    24481 cttcatacaa aatcaacgtg ctttcatgct cacgccggaa ttttgcctta tccgctgcat
    24541 tgcggtattc catagccacg ggccagagct gcttgtaggt agaaatattc tttatcagca
    24601 ccgccatatc cgcaagccgg tgttccatag ctttgagggt agcggcggcc tcgtcgctgg
    24661 cggcggtgat ctcggccact ttgctttcca aatccgggta ggtgtctatc ccatgttcag
    24721 tcaggaaatt caaagtcttt gcggcctgct tcaaattgtg gagcttcgcc cacctctcat
    24781 agcctgccga ctgctgggcc ttaatgttgt tttccagatc aatccgcaaa gaaatcccct
    24841 tgcgctcttt gggcgctctg gtgataacgc ggcctttgat acgctctttg atggcttcct
    24901 cggtgtaggc ttcccccaat gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga
    24961 aagaaataaa ctttcccggc ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat
    25021 catcaaaatc ttttgcctgc gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc
    25081 agctcgtccc tttccgctgg gcgtcccact cggcatagct cttgccctta ctctggccgg
    25141 gcttcaccac ggacaatccg ttttccttgc acagcctgtc gctggtgcgc cggatatagg
    25201 catagctctg tttgttggaa atgtacttgc gctgatttac catatccacg gcacagaaaa
    25261 taatgtgatt gtggacgtgg cccttatcaa tatgggtagt cagcacatag ggatatttcc
    25321 cttgcagcac ttcctcggca agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt
    25381 cgccgggggc aaaggattgt atcaggtggt ggcccagatt attgcccttt tggtatgcct
    25441 gcgccagcag catttcaaat tcaatgtccg ctgtttcata agagcaggca aaggaggcca
    25501 ccagcagctt gccgtcagtt ttgtccgggt tctcgatgta gtcaagggct tttttcagcg
    25561 ttgacttgat aggatgtatc tttgtaactg ccatatctgc gccagcgccc cctttatatc
    25621 ctcgatgtcc tgtgcgtaca ccggccccat accgttcagc ctctttgcaa tctgattgac
    25681 gttgacgccg atcttctgta tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt
    25741 gacgatatag ccgtcaatga gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag
    25801 cagcgccatt ttctgttcaa tcatagcccg ttcctccggc gtcacaggga cgcgcagcac
    25861 gatttttcgc tttctgtttg ccatgcttca ccgtcctttc cattcagggg tttggggatt
    25921 tccccaacaa gcaaaacgga cgcggcccgg cagggacggg gcaggccgtt tttccgggag
    25981 tggctacact ccctatgctt gctacgttac tgtttctcca ctaacaagac gtttccaaag
    26041 ccccaaacta cccgcaaaat agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta
    26101 cgctgtgcag ctcccgctaa aagtaaaaca gaggatgcca gttacacatc ctccgtttca
    26161 ttcttcttcg gcttttcaat tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg
    26221 tattcttcgg ggcagagttt cagcttgtgg ctgacccttt gccgcagttc gctttcctcc
    26281 cgcatgatct ccgggttaaa gtagcgttcc acaggaagcc cgcaggcttt aataagctgt
    26341 atgacaaccg gcagactggg aatggcccgt cgttttcgat gttggcaaga tagcgccagt
    26401 caatatttat tttctctgcc attgctttgc gcgaaacgcg ctttgcttgc cgggccgctt
    26461 tcacatccgc gccgaaagtt tcaaagccgg gacaatcttc aatattcgcc atatcgcacc
    26521 tcccacttac attgtatatt tcatactctg cccgtggaat gttggtatat gcacctttct
    26581 taaagtttat atttcacatt ttgtttcttt tattcttcga gcaaaagaat tataatgtac
    26641 cctgtggagg tgtactatgg actacatgac gttgaaagag gcaggcgaga aatggggtgt
    26701 gcccccacga tggataaact actattgtgc tgcgggacgc atccccggcg ctgtaaagat
    26761 ggcgacgatc tggctcattc ccaaaactgc ggaaaagccg attgacaggc gtacaaagaa
    26821 aggaaaggcg gttactgaat agtataatga aaccggcgca ttttggatat ggttttgaag
    26881 agagagaaag catggtattt tgtcacggaa aaactataag atgatagatg gcaggctgct
    26941 ccagaccgat aaaaagtatt ctcatctgaa acagaaagaa aagatttaca catggatgta
    27001 tgaagaagcg aaactttatc atgacagaaa tggaaaatgc ccgaaacaaa gacgaggaag
    27061 ctgctgctgt atcagccgta tatggtagaa ttgaaaaggc cggcatctgg attctttatg
    27121 gtgaagtctc cagacattgg ataactgctg gtctaatgcg cctttgagta tggggtgatt
    27181 gattttaata ccggggaggt acggcttttt gaggaaaata ttctgggatt tacatttggc
    27241 gattattaca tttcctgtaa ctgcaaggac acggatgatg aagatatgag gaatatagac
    27301 ttgctgattt tctactgtct tgaacggtat taggactgaa tctgctgcgc ggagtgcggc
    27361 aagagcgacc ggagggagta cacacaggcc ctgcctgtgt gaaaagccat accgcaaggt
    27421 gcattcgcaa aaaagaggcc gcgcattttg ctgaggcttg gggtgtcccc aacaagcaaa
    27481 acgagaacgc cgcaggcggg caagtggtgg atcagaatat ccgtaaagga tattctgatc
    27541 cattgcttgc cagtattatc gggatgtttt gacaaccgct gtaatcgaaa catcccaaac
    27601 attaagaagg tgttaattat tattatcttg atttaattcg tttaaaccaa taatatagat
    27661 acatacacca tgaaaagaag cgagtatccc aatgacggat gattaaaatc cgaaaagaaa
    27721 tcgctttgaa actacaggga aactacagac tgttatgtta tcttcttaaa atggagggat
    27781 ttttatgtcg atacgaattc tacttgtcga ggatgatgat catatctgta atacagtaag
    27841 ggcgtttctg gctgaggcag gatatcaggt ggatgcctgc acagatggaa atgaggcata
    27901 caccaagttt tacgaaaaca cttatcaact ggttattctt gatattatgc tgcccggtat
    27961 gaacgggcat gaacttttgc gtgaatttcg tgcgaaaaat gatactccca ttctgatgat
    28021 gacagccctg tcggatgacg aaaaccaaat ccgggcgttt gatgcagagg cagacgacta
    28081 tgtaacaaag ccattcaaga tgcagatttt actaaagcgg gtggaagccc tgctgcggcg
    28141 cagcggtgcg ctggcaaagg aaatccgtgt cggcaggctg acacttctgc cggaggattt
    28201 cacggtactt tgtgacggta cagagctgcc cctgacacga aaagaatttg aaatcctttt
    28261 gctgctggtg cagaacaaag gcagaacctt aacgcatgaa atcattttgt cacgtatatg
    28321 gggatatgac tttgagggtg atggcagcac agtccacact catatcaaaa atttgcgggc
    28381 caagctgccg gaaaatatca tcaaaaccat tcgcggtgta ggataccgat tggaggaatc
    28441 ataataatgg aaagaaaagg gattttcatt aaggtttttt cctatacgat cattgtcctg
    28501 ctactgcttg tcggtgtaac agcaacactg tttgcacagc aatttgtgtc ttatttcaga
    28561 gtgatggaat tacagcaaac agtaaaatcc tatcagccat tggtggaact gattcagaat
    28621 agcgataggc ttgatattca agaggtggca gggctgtttc actacaataa ccaatccttt
    28681 gagttttata ttgaagataa agagggaagc gtactctatg ccacaccaaa tgccaataca
    28741 tcaaatagtt ttagacccaa ctttctttat gtggtacata gagatgataa tatttcgatt
    28801 gttgctcaaa gcaaggcagg tgtgggattg ctttatcaag ggctgacaat tcggggaatt
    28861 gttatgattg cgataatggt tgtattcagc cttttatgcg cgtatatctt tgcgcggcaa
    28921 atgacaacgc cgatcaaagc cttagcggac agtgcgaata aaatggcaaa cctgaaagat
    28981 gtaccgccgc cgctggagcg aaaggatgag cttggcgcac tggctcatga tatgcattcc
    29041 atgtatgtca ggctgaaaga aaccatcgca aggctggagg atgaaatcgc aagggaacat
    29101 gagttggagg aaacacagcg atatttcttt gcggcagctt ctcatgagct aaaaacgccc
    29161 atcgcggcta caagcgttct gttggaggga atgcttgaaa atatcggtga ctacaaagat
    29221 cattctaagt atctgcgcga atgcatcaaa atgatggata ggcagggcaa aatcatttcc
    29281 gaaatactgg agcttgtcag cctgaatgat gggagaatcg tacccatagc tgaaccgttg
    29341 gacatagggc gcacggttgc cgagttgctg cccgattttc aaaccttggc agaggcaaac
    29401 aaccagcggt tcgtcacaga tattccagcc gggcaaattg tcctgtccga tccgaggctg
    29461 ctccaaaagg cactatccaa tgtcatattg aatgcagttc agaacacgcc gcagggaggc
    29521 gaggtacgga tatggagtga gcctggtgct gaaaaatgcc gcctttttgt tttgaacatg
    29581 ggcgttcaca ttgatgatac tgcgcttcca aggctgttca ccccattcta tcgcattgat
    29641 caggcgcgaa gcagaaaaag tgggcgaagc agaaaaagtg ggcgaagcgg tttaggactt
    29701 gccatcgtac aaaaaacgct ggatgccatg agcctccagt atgcgctgga aaacacctcg
    29761 gatggcgttt tgttctggct ggatttaccg ctcacatcaa cattgtaaat atttaaaact
    29821 taaatgattt t
//
LOCUS       DAYYNG010000017        28723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000017 DAYYNG010000000
VERSION     DAYYNG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.017"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..157
                     /locus_tag="ACHBFA_000634"
                     /pseudo
     CDS             20..157
                     /locus_tag="ACHBFA_000634"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            190..513
                     /locus_tag="ACHBFA_000635"
                     /pseudo
     CDS             190..513
                     /locus_tag="ACHBFA_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="ACHBFA_000636"
     CDS             533..1336
                     /locus_tag="ACHBFA_000636"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9394152.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /gene="fni"
                     /locus_tag="ACHBFA_000637"
     CDS             complement(1585..2649)
                     /gene="fni"
                     /locus_tag="ACHBFA_000637"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9394153.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="ACHBFA_000638"
     CDS             complement(2646..3731)
                     /locus_tag="ACHBFA_000638"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9394154.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="ACHBFA_000639"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="ACHBFA_000639"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9394155.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="ACHBFA_000640"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="ACHBFA_000640"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9394156.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5974..6633
                     /locus_tag="ACHBFA_000641"
     CDS             5974..6633
                     /locus_tag="ACHBFA_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9394157.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6718..7623
                     /locus_tag="ACHBFA_000642"
     CDS             6718..7623
                     /locus_tag="ACHBFA_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9394158.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="ACHBFA_000643"
     CDS             complement(7625..8257)
                     /locus_tag="ACHBFA_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9394159.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8408..8533
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="ACHBFA_000644"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="ACHBFA_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9394160.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="ACHBFA_000645"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="ACHBFA_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9394161.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="ACHBFA_000646"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="ACHBFA_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9394162.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10037..11375
                     /locus_tag="ACHBFA_000647"
                     /pseudo
     CDS             10037..11375
                     /locus_tag="ACHBFA_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            11423..13552
                     /locus_tag="ACHBFA_000648"
     CDS             11423..13552
                     /locus_tag="ACHBFA_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM9394163.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13527..14216
                     /locus_tag="ACHBFA_000649"
     CDS             13527..14216
                     /locus_tag="ACHBFA_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394164.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14624..15310)
                     /locus_tag="ACHBFA_000650"
     CDS             complement(14624..15310)
                     /locus_tag="ACHBFA_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9394165.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15446..15640)
                     /locus_tag="ACHBFA_000651"
     CDS             complement(15446..15640)
                     /locus_tag="ACHBFA_000651"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394166.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15690..16130)
                     /locus_tag="ACHBFA_000652"
     CDS             complement(15690..16130)
                     /locus_tag="ACHBFA_000652"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM9394167.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16134..16979)
                     /locus_tag="ACHBFA_000653"
     CDS             complement(16134..16979)
                     /locus_tag="ACHBFA_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9394168.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17128..18546
                     /gene="pepV"
                     /locus_tag="ACHBFA_000654"
     CDS             17128..18546
                     /gene="pepV"
                     /locus_tag="ACHBFA_000654"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9394169.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18622..19824)
                     /locus_tag="ACHBFA_000655"
     CDS             complement(18622..19824)
                     /locus_tag="ACHBFA_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394170.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19811..21598)
                     /locus_tag="ACHBFA_000656"
     CDS             complement(19811..21598)
                     /locus_tag="ACHBFA_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFM9394171.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21611..22564)
                     /locus_tag="ACHBFA_000657"
     CDS             complement(21611..22564)
                     /locus_tag="ACHBFA_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9394172.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22749..23099)
                     /locus_tag="ACHBFA_000658"
     CDS             complement(22749..23099)
                     /locus_tag="ACHBFA_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9394173.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23299..24378
                     /gene="pepA"
                     /locus_tag="ACHBFA_000659"
     CDS             23299..24378
                     /gene="pepA"
                     /locus_tag="ACHBFA_000659"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9394174.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24521..24841
                     /locus_tag="ACHBFA_000660"
     CDS             24521..24841
                     /locus_tag="ACHBFA_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9394175.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24863..25327
                     /locus_tag="ACHBFA_000661"
     CDS             24863..25327
                     /locus_tag="ACHBFA_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9394176.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25523..26128
                     /gene="ytpR"
                     /locus_tag="ACHBFA_000662"
     CDS             25523..26128
                     /gene="ytpR"
                     /locus_tag="ACHBFA_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9394177.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26167..27456)
                     /locus_tag="ACHBFA_000663"
     CDS             complement(26167..27456)
                     /locus_tag="ACHBFA_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM9394178.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27884..28363
                     /gene="rlmH"
                     /locus_tag="ACHBFA_000664"
     CDS             27884..28363
                     /gene="rlmH"
                     /locus_tag="ACHBFA_000664"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM9394179.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         9084 a         5359 c         5841 g         8439 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcta gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
    10561 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
    10621 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
    10681 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
    10741 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
    10801 gggtcgttct tttagatctt caaatcatct taggttttat tctagcaggt atctgggcat
    10861 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
    10921 tatttacaga tggcgcagtc gatgttgcac caatggttgg tttcttgatg accttagcga
    10981 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
    11041 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
    11101 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtgttagctg
    11161 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaattaca acgattgctc
    11221 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
    11281 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcggt gcgatcttat
    11341 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
    11401 tagataaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
    11461 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
    11521 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
    11581 aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
    11641 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
    11701 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
    11761 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aaaacttaaa
    11821 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
    11881 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
    11941 atctgtgaaa gcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
    12001 aagaagaact cggacagcag caatcaacgc atctatttta ccaaaaatgc tagatacggc
    12061 aacctcgaca gaagactccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
    12121 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
    12181 ttccggtcct gctgcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
    12241 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
    12301 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
    12361 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
    12421 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
    12481 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
    12541 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgttct
    12601 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
    12661 acaggcatta gcagactatt gtcatacgac ggttgaagac atcgcagagc agatcatgga
    12721 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
    12781 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
    12841 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
    12901 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
    12961 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
    13021 accagaatcg atcgaagtcc acgtagaaat cgatccacat acttcaaaag taacggctat
    13081 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
    13141 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
    13201 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
    13261 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
    13321 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
    13381 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
    13441 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
    13501 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
    13561 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
    13621 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
    13681 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
    13741 gagaaatctg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
    13801 aacgaatcac ttttgcaact tatgcagaag atgaagggat ccgattgatg acggagccgt
    13861 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
    13921 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
    13981 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
    14041 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
    14101 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
    14161 aagaaataaa agaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
    14221 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
    14281 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
    14341 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaaaggtg ttcaaaagct
    14401 tgcatctgcg gtaataagaa cagccaagtt aaaaacataa gaagctattt ttacttggct
    14461 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
    14521 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14581 tatttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
    14641 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
    14701 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
    14761 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
    14821 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
    14881 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    14941 cttttttatt ttgagcgatc actgcctgat cggtttgttc tagtattttt tctagtttag
    15001 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    15061 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    15121 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    15181 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    15241 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    15301 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    15361 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    15421 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
    15481 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    15541 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    15601 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    15661 atacaagaaa aatgcttaga cggtatttat taagaattta catcatccaa agaagcgtac
    15721 gcttggaata actgttctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    15781 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    15841 cccacaattt cgtagctaaa atccaaccgt gttcccgtat acttttggat agttgggtgg
    15901 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    15961 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    16021 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    16081 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    16141 tcgttttcca ttgatttttt acaaaaataa ttcatccact tcggaagtgc ttcgctgatt
    16201 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    16261 taaacagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    16321 atcccagcta aagtgtcacc catgccgcct gttgccatag cagcagaacc taaaggattg
    16381 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    16441 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    16501 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    16561 aaaagatgat tagcaacaag taaacttaaa gcagagccat caatcaccag ccattgttct
    16621 tttgattgtt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    16681 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    16741 gtccaatcta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    16801 tcagttacta cagtcgtgag acccgctcca ctgtgtacgg cagctaaagc actcataatg
    16861 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    16921 tgtgaaatct ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    16981 gaaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    17041 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    17101 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    17161 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    17221 gattcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    17281 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    17341 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    17401 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    17461 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    17521 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    17581 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    17641 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    17701 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    17761 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    17821 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    17881 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    17941 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    18001 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    18061 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    18121 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    18181 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    18241 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    18301 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    18361 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    18421 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    18481 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    18541 aaataaaaat actaaaaaac tccggctatc tctgacagct ggagttttta tgtttaaaaa
    18601 tgaattagta catcggtatt cttaaaagta atttagttcg tcttttgatt tctcaggagg
    18661 gatcaaaaag gcgaaagcac taagtatagc aaaacttgtg atgatttgcg tacgatacgt
    18721 aaaccaataa tgaatctgtg agtgattagc taaaatattg taccaaatat acggcgttac
    18781 agcagctaaa acaaacaaaa ctagaagttg gaacggacgc cagctatttc gtttacgata
    18841 agcaaaatag agaaaaacaa gacaagtaat caatagcaat aatattgcta cttttggata
    18901 catcaatcta atattgcttt tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc
    18961 ttccgttcga aagaggattt ggttgattgc atcttttaca atgtttttgt tcaatatgat
    19021 cgatgatagg atccatttag ccacccatgt caaaccatag cctaatcccc aaaagatacc
    19081 ggtaaaaaca aaactgccta cttgtttgct caatgaactg cgtttatcca ttaaatactt
    19141 atttgttaca taataaataa tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa
    19201 caagtcaaaa aagttagcta aagaaccaat gacaaagaaa tacaagaata attgttggtt
    19261 ggtgttggag agttgtggtt ttgttaaaaa tagtaataca gacaataaag tcaaaatcca
    19321 aatatttgaa aattgcatac tgaagaaaaa ggttgcagca tttcctatag caagcgaaat
    19381 caacaaggct gtcgttaaat agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc
    19441 aatcagcaga aaaagaatgg ctgcgtaaat cattcgaata ctccccaaac tcatccctag
    19501 cagtaaaggt cgtaaaaaaa gctgataacc atgccaataa cggggataat ccgccataga
    19561 catcgcgttc tccaaggagt tcaattcagg atctgctttt gtttttcgta tcatcaaatg
    19621 atcagtaaaa ttatctaacc gagtgcctag catatttcct tgattgatag aaggatacat
    19681 tccttctttt tcaaccatct gtagagattt ttctgcattc gattcaatag agtgctgcgg
    19741 aatgaaaaaa gtaattgtca gtagtacgca atagctagcg atcaagagaa caaaagcaaa
    19801 aaatatcttt ttaaatagtt tcatgagtat ctcctatatc tgttttcatt ttttctcttt
    19861 tatctagtaa aaaaggagag aggatataaa caggtagacc taatgctaaa acaaacacat
    19921 accgtaaact aaaagccaca ggagtagcaa tcataatagt agcaaaaaca agcataccgg
    19981 gaagaaaaag gagtaaatac cgatattttt tcttaaagat tgctaaaagt gcactgccga
    20041 tagctaacca cagtaatgtg ccggaaccaa agaaatcacg agcatcgatg atttttttca
    20101 ttcctgacaa tccagtaaga tgttcaatca aatctacttg cttcatacca taattattcg
    20161 tgtttacacg agtatctaaa aagccgtaac tattttttgt accaggtacc cagaacccga
    20221 acgtttctaa aatataagca tctgtataga tttttatatt acttggtaaa tttcttgccc
    20281 atagtgatag aaattctttt ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc
    20341 acttgattga gtctgcaaga ctaggagaat acgcttcata atcttttatc gggagtagct
    20401 gattcatgaa tgctttatct ttcttagaca agtcaccatc cataacaata gttctagaaa
    20461 tttgctgcaa aggaacacct aattgttcag cagtttctgt aggaatagca taacgagcca
    20521 tcaacgggtg gatgagtagt ccagcaatca ctgtataacc cagcaccact tgaaagaatg
    20581 cttgatacct tttttgtttc aaaataatca agccgcaaat caaacctgtg ccggttacta
    20641 cataaatccc attatttctt agataggtgg tcagtaaagc aaggatgatt acgtaaatct
    20701 gattgagaat cgttggcttt tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata
    20761 aaaagcaagc gatagagaac aaagtatctt tttgcatgtt caaggcgtag attggaaaaa
    20821 ccggtgcaaa tgcaaaatag cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt
    20881 ttaacagcca gcaaagagaa taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg
    20941 aaaataaaaa tacaccgtga ttgatcgtat cagttggtaa atcatgaaca aatagcttga
    21001 ccagtaaggt gaataagact ggatgatgat tgcttatagg aacatcaccc atagattggg
    21061 cgaccgaact taacgaatca ggcaaaacca ctccaggata caatgccaac agataaggaa
    21121 gccaacacag aactaaaaag acagtaaaga ataaaaaata ttttcttgaa gagaaagcct
    21181 gtgtgttttg agcagtaaaa aatgtttcca gttttggcat tgataaataa atcgattgta
    21241 aaaataaaaa agaatacaca aaaatggaga aataaacaag aaagtaaaaa atacttccat
    21301 cagcaatcgt tgtctttgta tagtttgctg agacatactg aaattgtatc tgtctgccaa
    21361 tagaaagtgt aagagcaaaa gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt
    21421 tttttattga aaacaactct attccatatt gcttaaaagc aagtagcaaa accatggcta
    21481 aaccaatgat tactgttgga ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac
    21541 ctgacgcaaa taaagttgca aaaaaagtag gaaatagatt ctcctttttc ttatgcataa
    21601 aaaaggcctc ctacttcatt tctttatttt gatattcgtg gtagacatgg tataaattca
    21661 attcatattc tttcttggca tttgtgacga ccgtatccaa aatcaaccct gttactaatg
    21721 aaagaagggc taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa
    21781 agcttgtcag catatattca cgaatcacag gaatccccat gaccaatcca aataaaaaga
    21841 agaagatggt ccagatccca aagaaaagca aaggtttata atctttgcac attttgatga
    21901 tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctaccttcag
    21961 gacgatcgcg ataatcgatt ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa
    22021 tcgtcaattc tgtttcgatt tgaaaaccag aactcataat agggaagctt ttgacaaaaa
    22081 ttcgattgaa tcctcgatag ccggtcatga catcgcgaat ttttgtttta tataagtaag
    22141 taattgtatt tttaaccaga ttattcccaa aatcatggaa aggacgctta ttttcatcaa
    22201 aataggttcc atttgataga cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt
    22261 tctcaagcaa accatgtgca gcttctgcag gataagtatc atcaccatct accatcaaat
    22321 aataatcggc atcaatatca aaaaacattt gacggattac attccctttt ccttgacgtg
    22381 gttctttctt tacgattgct ccgccttgaa cggccaattc gtatgttcga tcggtcgaat
    22441 tattatcata gacataaata tctgcttctg gtaattccct tttaaaatct tcaataacag
    22501 ttgaaattgt cgcctcttcg ttataacaag gaatgagcac ggcaattttt tcacgtgatt
    22561 ccatttgtca ctctccttat ataataaaac taatcttatt tcaataatac cgtataataa
    22621 ctacaaatac tagctattcc aagaggaaat agccctattt ttataaaaat cacccactat
    22681 aaaagaagga taggatacct ctcttttaga tagtgggtgc gtattcgtta tttcattttt
    22741 ttgacgtatt aatctattgc gccctttttt cttgaaatgt ctaatacggt tcctgtatga
    22801 gcatctgcta aaaattcata ataaacagtt tcgccatctt ctatccggga aagcccaccc
    22861 cgataagcac tcgaccgaac ggcgaatttc cgaataggtt ctttttcaaa agagatccaa
    22921 gcaccttcga ttgcgccttc ttttaaaaaa gatgttttga tattttcaag cacttggtct
    22981 gctgataaca cttttttgcg atgataccaa atcgttgaag ctaccccact caataatcct
    23041 agaccgatcc caacggctaa cccgccttta aaatagctta attcgctttc ttcatacatg
    23101 tggatatacc tccttcttct cacttgctac tctttgtgtt tattttacca cactctcata
    23161 ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat acgatcggtc
    23221 tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag tttagtctga
    23281 aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa
    23341 ttacaaggaa caagcggttt tgaacaagac gttcgcgcct atatggaaag tcatatggaa
    23401 ccgctggtag atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat
    23461 gaagaagcgg atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg
    23521 ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct
    23581 tacgttgttt ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt
    23641 atttcttctt ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa
    23701 gtaacagata ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt
    23761 gttcttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag
    23821 aatatcatca gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta
    23881 gaagcgttac aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa
    23941 gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt
    24001 gcagtagact gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta
    24061 ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt
    24121 gagtacttgc tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa
    24181 ggcggtacag atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt
    24241 ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa
    24301 gctgcacgag aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg
    24361 atcgtatacg ggaaataacg cgatagtgaa actataaaat ctggcaaaag caaccttttg
    24421 tcagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    24481 ttaattgaaa aatacataaa cagaaagtag aggaaaaaga atgattatcc caaatagttt
    24541 agaagaatta gcaggctacg tagaaactgg aaaaagtgta tttttcttta ctgccggctg
    24601 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa atcgaaaatg acttttctga
    24661 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    24721 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    24781 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa taaaaggctg
    24841 acatctgtta ggaggggtca agatgaatca agaatataag aacattttag taggtattga
    24901 cggtagtgaa caagcacttg ctgctttcaa aaaagcgtta gaagtggctc gccgtaataa
    24961 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataatttta tgggctatgc
    25021 tcctattgat caaaatctag tcgatcagca aactgaaaca gccaagcaaa tgatcgaaga
    25081 atgtaaagaa tatggaaaat ctgttgatta cacaaatata gaaggaatta ttgcatacgg
    25141 ctctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgcaatcg atctaatcat
    25201 ggttgggcag tctggactaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    25261 cgtcatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    25321 taattaagca agtggaactt gtataacctt tttaatcaaa cagtaaaggg actgtgacaa
    25381 aagtcttgtc acagtccctt gttacgaata aatggtgttc cagaagaagc ttcttcggaa
    25441 ataaagcatg aaaaaaatct catgcagtga atgcaatttt ttgttaagat aaatctcagc
    25501 aaaggataat ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac
    25561 tttgatggtt atcatagccg acgataaagg aacagaaaac actgtcgaac gtaaatggaa
    25621 tgttgctcgg atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc
    25681 cgatcatcta acaattgaag ggaacggcca agtaagctta acagatgaag aattagcgaa
    25741 attgaatcga ttgattaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc
    25801 gaagatcgtt gtcgggtatg taaaaacatg cgtcccgcat cctgactctg accatttatc
    25861 gattactgag acagaagtgg acaatgggac agtattgcaa attgtttgcg gtgcaccaaa
    25921 tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga gccatgatgc cagatggaat
    25981 gatgatctgg ccaggaactt tgcgaggggt agccagctat ggaatgattt gttctgctcg
    26041 tgaattgcat ttgccaaatg ctccagaaaa aaaaggaatc ttagtattgc ctgaggatgc
    26101 cgtagtcgga gaagtatttc caaaataaag aggttgtgac aagtctttgt cacaacctct
    26161 tttttattaa ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc
    26221 aacagtagtt gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac
    26281 ttttttctta tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat
    26341 cttagtaatg acgtcgtatt gtttcaaccc agctttttct gccggtgtag ctgtttggac
    26401 atttgtcacg atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg
    26461 ctgagtagaa acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt
    26521 tccgtctttt tccagttggt tgatgatatt cactacatcg ttacttggga tggcaaatcc
    26581 cattccttct acgcttacat tagaagtcga ttcagaagta cttgcgatct tacttgagtt
    26641 gataccgata acttgtcctt cgatattcac taaaggacca ccagaatttc ccgggttgat
    26701 cgctgcatct gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt
    26761 tacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca gaattcgcat attcagatcc
    26821 taatggggaa ccaatcgcaa tcgcaggttc accaaccttc aaagcacttg agtccccaaa
    26881 agaggcaacc gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataggc
    26941 atctgttcca actaattcag cttttacctt cgtaccgtct ttcatcatga cttccaaacc
    27001 ttgctgtcca tctactacgt gattgtttgt gactacataa gcagtattcc cgtctttttt
    27061 gtagattacg ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact
    27121 ttcttcttgc tgcccgaata atccgccaaa accgcttgat tggttttgac tttgtaagtt
    27181 gataacagaa acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac
    27241 attgactttg acattttcca caactgtttc accggcagaa ttttgattgc cgcttgtagc
    27301 agtagaacta ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc
    27361 tccaacgatc cctccaacaa gccccagacc caacttacgc cagaggctat tatttttatt
    27421 ttttttcatc tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca
    27481 tttatctact gttagtatag tcttatttgt tgactttagt ctactttgag actttgaact
    27541 ttttatgaaa aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt
    27601 tccacagata ttgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat
    27661 ccacagcctg tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt
    27721 gatgaaataa ggtaaaacaa cgctgttttc tgtggataaa cctgtggatg ttgtgcataa
    27781 ctgttgggga aatcttttta acaaaaatag tcaaaataaa catatagacg aaaaaattga
    27841 ttataataaa caaagtgagc gtgaagatca aaggagagaa aacatgaata tcaaaattat
    27901 ttcagtagga aaattaaaag aaaaatactt gatccaaggt atcaatgagt atgtcaaacg
    27961 gctgaatgcc tatgcgaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa
    28021 tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa ggagaacgga tcttagcgaa
    28081 aatcaaagaa caagaatacg tctatggctt agcaattgaa gggaaaaatc ccacaagcga
    28141 atcttttgct aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt
    28201 tatcggcggt tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc
    28261 ttttggcaaa atgacgtatc cacatcaatt gatgcgcctg atcctcgtcg agcaaatcta
    28321 ccgtgccttc cggatcaacg cgggggctcc ataccacaaa taatgatatt ttttaagaga
    28381 aaaaattatc cctattttaa ataatagcaa taggtagtgg tagggagaaa tttataaata
    28441 acttttttat ttgctttggt agccaagtaa gttgcaaaag aaaaatagat actgttaaaa
    28501 agataatgta gacatagaaa caaatacgta aaattttagt tagaataatg gcaaggagaa
    28561 aaaacatgat agatgaaaaa aagtgtttaa aaaatctatt ggaggatatg gatgatattg
    28621 aagaaaaact atctaaatgg actaggaaaa caaatatatt tcagatactt aagctatcta
    28681 aaaatgaaat aagacatagt aattttttag cttttttgtt tgc
//
LOCUS       DAYYNG010000018        28561 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000018 DAYYNG010000000
VERSION     DAYYNG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.018"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..193
                     /locus_tag="ACHBFA_000665"
     CDS             <1..193
                     /locus_tag="ACHBFA_000665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394180.1"
                     /translation="KPFSQNFSYYSKLSDFFTTSSLNGVQKLASAVISPTKRKIGGAI
                     FVWLFLLLDAEQLFHNLFL"
     gene            333..1202
                     /locus_tag="ACHBFA_000666"
     CDS             333..1202
                     /locus_tag="ACHBFA_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9394181.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1411..2436
                     /locus_tag="ACHBFA_000667"
     CDS             1411..2436
                     /locus_tag="ACHBFA_000667"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9394182.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2475..3425
                     /gene="manA"
                     /locus_tag="ACHBFA_000668"
     CDS             2475..3425
                     /gene="manA"
                     /locus_tag="ACHBFA_000668"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9394183.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3519..5222
                     /locus_tag="ACHBFA_000669"
     CDS             3519..5222
                     /locus_tag="ACHBFA_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9394184.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5224..7257
                     /locus_tag="ACHBFA_000670"
     CDS             5224..7257
                     /locus_tag="ACHBFA_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM9394185.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7268..7708
                     /locus_tag="ACHBFA_000671"
     CDS             7268..7708
                     /locus_tag="ACHBFA_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9394186.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7908..9035)
                     /locus_tag="ACHBFA_000672"
     CDS             complement(7908..9035)
                     /locus_tag="ACHBFA_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9394187.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9038..9715)
                     /locus_tag="ACHBFA_000673"
     CDS             complement(9038..9715)
                     /locus_tag="ACHBFA_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9394188.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9979..11745
                     /locus_tag="ACHBFA_000674"
     CDS             9979..11745
                     /locus_tag="ACHBFA_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9394189.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11760..12197
                     /locus_tag="ACHBFA_000675"
     CDS             11760..12197
                     /locus_tag="ACHBFA_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9394190.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12210..13361
                     /locus_tag="ACHBFA_000676"
     CDS             12210..13361
                     /locus_tag="ACHBFA_000676"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9394191.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13423..14121)
                     /locus_tag="ACHBFA_000677"
     CDS             complement(13423..14121)
                     /locus_tag="ACHBFA_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM9394192.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14114..15466)
                     /locus_tag="ACHBFA_000678"
     CDS             complement(14114..15466)
                     /locus_tag="ACHBFA_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM9394193.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15689..17089)
                     /locus_tag="ACHBFA_000679"
     CDS             complement(15689..17089)
                     /locus_tag="ACHBFA_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9394194.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17250..17987
                     /locus_tag="ACHBFA_000680"
     CDS             17250..17987
                     /locus_tag="ACHBFA_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM9394195.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18112..18591
                     /locus_tag="ACHBFA_000681"
     CDS             18112..18591
                     /locus_tag="ACHBFA_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM9394196.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18588..19355
                     /locus_tag="ACHBFA_000682"
     CDS             18588..19355
                     /locus_tag="ACHBFA_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM9394197.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19585..20154
                     /gene="lepB"
                     /locus_tag="ACHBFA_000683"
     CDS             19585..20154
                     /gene="lepB"
                     /locus_tag="ACHBFA_000683"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9394198.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20258..20860)
                     /locus_tag="ACHBFA_000684"
     CDS             complement(20258..20860)
                     /locus_tag="ACHBFA_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9394199.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20966..21196
                     /locus_tag="ACHBFA_000685"
     CDS             20966..21196
                     /locus_tag="ACHBFA_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394200.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21187..21435
                     /locus_tag="ACHBFA_000686"
     CDS             21187..21435
                     /locus_tag="ACHBFA_000686"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9394201.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21541..22125
                     /locus_tag="ACHBFA_000687"
     CDS             21541..22125
                     /locus_tag="ACHBFA_000687"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9394202.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22128..23201
                     /gene="prfA"
                     /locus_tag="ACHBFA_000688"
     CDS             22128..23201
                     /gene="prfA"
                     /locus_tag="ACHBFA_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9394203.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23194..24033
                     /gene="prmC"
                     /locus_tag="ACHBFA_000689"
     CDS             23194..24033
                     /gene="prmC"
                     /locus_tag="ACHBFA_000689"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9394204.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24026..24205
                     /locus_tag="ACHBFA_000690"
     CDS             24026..24205
                     /locus_tag="ACHBFA_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394205.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24227..25252
                     /locus_tag="ACHBFA_000691"
     CDS             24227..25252
                     /locus_tag="ACHBFA_000691"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9394206.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25427..26671
                     /gene="glyA"
                     /locus_tag="ACHBFA_000692"
     CDS             25427..26671
                     /gene="glyA"
                     /locus_tag="ACHBFA_000692"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9394207.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26824..27771
                     /locus_tag="ACHBFA_000693"
     CDS             26824..27771
                     /locus_tag="ACHBFA_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM9394208.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27905..28534
                     /gene="upp"
                     /locus_tag="ACHBFA_000694"
     CDS             27905..28534
                     /gene="upp"
                     /locus_tag="ACHBFA_000694"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9394209.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         9718 a         5048 c         5862 g         7933 t
ORIGIN      
        1 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc
       61 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
      121 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
      181 cctcttttta taagagtttt tactcattaa tttacaaaaa acaagcaaaa tgcgaatttt
      241 ctcacatttt ttttaacaaa atcaaagtta ttgcttgaaa gaagaagaat ccatcgttaa
      301 aataaagctt gacgtagagg ggagcaagaa atatgaaaga ttttcaattg ggggctgcca
      361 cctatttaaa aagtgtagcc attcgggtga aagaccgaga taaaatgatt gatttttaca
      421 agaacgttgt tggctttgat ttgaaaagag aagaaaacga attagccatt ttaggaacag
      481 aagaagctgg aagtgaaatg ttgctgctgg aagaaagtcc tagagcgaat gatcacatgg
      541 gggtgatcaa aaagctgaac cgtttttcat tgattatccc tagcacagaa gaaatggcag
      601 atatcctttg tcgtatccgt aaaatgaatt atccaatcaa agaagcttta gaagatcatg
      661 gacgtatggg gattttgctc gtagacccag aagagaatga attggaaatt taccatgaag
      721 agaataagaa cgaccaaaga gaaattccag aaacaatgga tcaagaagca ttattaagaa
      781 aatctgaaga aaaattcagt aaactatcaa caggttctta ttttgaaaaa atccatttaa
      841 atgttactaa cttaacaaaa gaacgccaat ttcttaatca tgtacttggt ttgaaggtac
      901 ataatgaaga agagggcatc catgtattga atgaaggaga tttccatgtt ggtttgacag
      961 aagctagcgg aggcgcaatt gatttgccta cagatgaagt gattggactt gattttctga
     1021 aattcatgat cgatactgaa agcatggaag gactgattgc tcacttgcag caactatcag
     1081 ttgatttctt cttagacaaa aagcacacag tactcacaat ctatgatgct ataggaatcg
     1141 aatggtggtt cgtcagtgag ccaaaagaaa agaataaaac tgacattcga tgcgacgctt
     1201 aactttttct ttcttaacga ttgtcgcatg tctcgtcatt ttatgaacgg tcatactatc
     1261 gcttattcta gagatagaaa gagaaaatag tataccgggg gaaactgcgc ggtttttcgc
     1321 gcagtttttt tgtgtaaaaa aggttgattc ctcatagatt aagctttaag cttggactgt
     1381 aagaatctga tagaaaagca ggagaaaaag atgtatccac acacgaaaca gcttattttg
     1441 aaaaagatgg aagaaggatt ttatccaggt gtagcagtcg ccttcataaa agaaaatcaa
     1501 gtagaaacat ttacagccgg ctttgcccaa gtcgttcctg aaaagcagct gatgacagaa
     1561 gatctgttgt ttgatgcagc ctcactgacg aaagtcgttg caaccacttc attagtttta
     1621 cgtctattag aagatggaaa gatagaactc gatcaaccgc ttcataaata tcttcctgaa
     1681 tttgaaaatc agacaatcac tttgcgtcat ttactgacac atacttctga tatcaaaaca
     1741 tggataccaa acagagataa gctatctcag gaagaattga tcgccgctta tttgaagcta
     1801 gaaccaggtg agcaactagg aaaagaagta aaatatacag atgccggtac gattcttctt
     1861 gggttgatgc tagaggaaat ttaccaagaa actgcagtgg aaatctttca agaacaagta
     1921 ttggaaccat taggaatgat gaacagctta tttcttcctt ttccttccaa aaaaatcgtt
     1981 cccacagaac aactgccttc gggtgaggtt ttgcgtggac aaactcatga tccgaaagca
     2041 agagttttgg ctgaacatgc aggtaacgcg ggtctcttta ttgatttgaa agacagcatt
     2101 acttttgtcc aaatgtattt gaacaaggga aaaatccgta atggtcgttt tttagaagaa
     2161 gagacgattg attctttact gcaagatcaa acaccgaaaa aaaatggcta tcgttcttta
     2221 ggatgggact taaaaaagga tgtagaagac ggtcatgcga ttctttttca tactggttat
     2281 acaggtacgt ttttcttgat tgatataaaa acacaagaag cgttgatatt tttgtcaaat
     2341 cgcgtacatc ctgtcgatca tcgtgcggag tatctcatat gtcgagatga aattgttgaa
     2401 aactttttga aagagaaagc ggtattaccg aaataatgct ataatagcta tgaaaaatga
     2461 gaggagtttg aattatgcag gaacctatgt ttttaaaacc agtttttcaa gaaaaaattt
     2521 ggggaggaag tcgtttacgt tcagtctttg gttttgatat ccctaacgat aaaatcggag
     2581 aagattgggc gatcagtgcg catcctcatg gtgtcagtgt agtagaaaat ggcgaattca
     2641 aagggaaaag attagatgaa ctatggtcag aacacaagga attgtttggc catccttcag
     2701 aaccagtgtt tccattattg atcaagatct tagacgcaga agatgatcta tctgtacaag
     2761 tacatccaga tgatgcctat gggatgaaac atgaaggtga attaggaaaa actgaatgct
     2821 ggtatatcat cgatgctgaa cctggtgcag aaatcatcta tggtcatcat gcaaagacaa
     2881 gagaagaact agctgagatg atcaaagatg ggcgctggga tgaccttttg aagaaagtac
     2941 cagttaaaaa aggtgatttc ttttatgtac caagcggaac gatccatgct attggtaaag
     3001 gaatcatgat tttagaaaca cagcaaagtt ctgacacgac ttaccgcgtg tatgactacg
     3061 acagaaaaga tgcgcaagga caaacaagag agctgcatat ccaacaatcg atcgatgtaa
     3121 caactgtccc tgcgaaaaca ccagaattac aaatcaaaga agtccgaaaa gggaattcat
     3181 cgatcgtcac ttatttagaa actgagtttt tcaatgtata tgagtgggat attaaaggaa
     3241 tcacatcttt caaaaaacaa gccccttata cattgatgac ggtaatcgaa ggagcaggag
     3301 accttgtcgt tgacggaaaa acatatccat tagaaaaagg aacaagctgt attattccaa
     3361 atggcgtttc tgagtgggcg gtccaaggtg aactagcaat cattgcgtct gaaccaggca
     3421 aatagttccc agatagctga aagcagcaga cggtttttcg tttgctgctt tttctacata
     3481 aaatccgtcc atcaataaat agaagtctgg tgaaaaagat ggaatacaaa tggaaagcaa
     3541 acaacagcag tcgcgtgtct atgaaacaac tgagaaaagc tggacatcac ttaaatgaaa
     3601 tgatcatgcc aaatctaagt ctgtttatcg cttggggaat cttgtcatta gcaacaaaag
     3661 gtgtgtctgg cgatttgcac accacattag aagaggtatg ccattggatg atccagctgc
     3721 ttttacccat cttgatcagt tattcaggcg ggaaatcggt agggggacaa aaaggggcag
     3781 tcgccggtgc aattgcttct ttagggttga tcgctcgttc caatgctcca caaatcgtag
     3841 gagcgatgat tatcgggccg cttgccggca tcgtatatga acaatttgtt tctagattcc
     3901 atgtgaaatt caaggctgga tatgaaatgc ttgtcagcaa cctactagta ggactgactg
     3961 gtagcgttat ttgtataatt ggaatcgttt ttattgaacc agttttaaat agtgttcact
     4021 tgttgctcgc ctccgttgtg tcttggctag tcacaatgaa tctgttacca cttgtcagtc
     4081 tgatcttaga acctctaaaa gtactatttt tcaataatgc aatcaatcat ggtgtattga
     4141 cacctttagg aatcgaattc tcacaaacag tcgggtactc ttccttattt ttgatggagg
     4201 caaatcctgg acctggttta gggatcttac tttctatcct ttgttttgca gaaaaacagg
     4261 aaaaagtaaa tgcgagtgga gcattgatga tccatgcgat tggcgggatt cacgaaatct
     4321 attttccatt tgtcttgctg cgaccgtatc tatttttagc tgtaatggct ggtggggcaa
     4381 gcggcacgtt gatatttcaa tggttagata cgggattagc tgcacctgta tcgcctgggt
     4441 cactactcgt gatttttgcc aatgcgcctt atccacaact agctggggta agtataggga
     4501 tagcaatcag tacatttgtg accttcattt gtgcagcgat actattgaaa ttaccaaata
     4561 aagaaaaaga gaacaaaacg gtcaaagaaa ggggagaaat cttggaacca tctatcattc
     4621 attccgtcat ttttgcttgc gactcaggta tgggatcaag tgctatggga gccgccctgc
     4681 tgaaaaagaa attagcagca gaaaaaatcg atctttctgt cggatacagc tctatctatg
     4741 aattgaataa ccaagtaaat cagctggtta tcgtgcaaca tgaactagcg ggtcttgcaa
     4801 gagaaaaagt acctcaaagt caaatctatg tactggatca ctttttggaa acagatatct
     4861 tgctgtcacg aataaaagaa gcgatccaat ttccaaagcg ggaagaaaaa aaagagatag
     4921 cagatatcac agaacaaaaa acgtcgcctg ctaatattgt atttttgtat aaaaacaaca
     4981 gacgcggctc acaaactatg ggcatgacga ttttaagtca attagcaaaa aaagcgaaaa
     5041 aacagtttgt tatctcaaaa gaatcaatag aaaaaatcaa aatccaagaa gaaacgattt
     5101 atatcgcaca aagagaacta gctgtaactg aacagctaga agaaaagatc cctcggctag
     5161 ttctagttga acattgggta gctacgaatg aatacgaaaa actcgtgaag ggagaaatat
     5221 aagatgtatt tatcgcccag agaaaaacaa ttattagcag aatttctcag tagcccgtca
     5281 cctgtatcta tccaaaaaat gatgaattta ttgaaggtca gtaaacggac tatttaccgc
     5341 gaattagata atcttgaagt ctcattgaaa tcggtaaatg cttctttgaa aaaagcagct
     5401 agaggctcat ttgtcattga ggcatcagaa gaagtattag agcaattaaa agaaaataca
     5461 gatgggaaaa gtaatgactt atccactgct aaacgacaac acgcgatttt agccgagttg
     5521 ttactgacga cagagccgtt gagtcttgct catttcatgg aatcatatcg catcagtaat
     5581 accacttttt atgcagatat caaacagttg gaagaaagta ttcgccaact gcctttggaa
     5641 atcattcgga accagggata cgagatcgaa ggaccggaaa aataccgccg gcttctgaca
     5701 gcgaatgtat tagaattaga aatcaatgaa tatgaacttt ttcatagtat ttctttcgat
     5761 gcatcactga attatttttt tcgattcgtt gatccgcaac atctatctct tgcacgcaaa
     5821 gtaatcgggg aagaattgat ccaaaaaaag accaatctaa gtgatagaaa gcttgaacat
     5881 ttggttttga tgctgacatt gacgatggag cgtgtgacga aaaatcattt actgacaaat
     5941 gagacatata atagtctggc aaacaaagaa ttactacaga cagctaaacg attgttttca
     6001 cggatcgctt ctgagacaaa acaactttat ccagtaaatg agatcgtctt ttttgcagga
     6061 ttattaaatg acttcacgaa ctcatttgat cagaatttct ttgaagagac gttcgataca
     6121 cagcttgctt atttagtaaa acaactgatt caagaagtaa gtgaagagac gaatgtccat
     6181 ttttatgaag atgaaacttt gtacaaaatg ctgttgactc atttatctgg tgtgttttca
     6241 agagccgttt tacaagaaga gacactgacc aatccgattt tagaaaaaat catggttcaa
     6301 tacgaggaag ttgcgaatgc aatccgtaaa gcagtagagc ggatttttca agaaaaaaaa
     6361 ttatccgaag aagagatcgc ttatatggtc cttcattttg cgaattcatt ggaacgaagt
     6421 ccgaaagaaa gcaatatcag tattgcagga ttttcgccaa gtggattagc ttctactagc
     6481 atgttggaac tacgattgaa aaggtatttt cccttcatcc aacaaattca tttctatcgg
     6541 attgctgatt taaaaaaaat cgatttacaa gaagaatatg atgttgttat ttctacatct
     6601 attttaccag gatacggtgg aaaatatatt ttagtctccc cgctactgct agaagatgaa
     6661 gtcaagcagc taaaagaagc tttttcgcat attgagaaga aaaaaaggca aaaaattacg
     6721 tataacagca ataacgagtg gatgatagaa gatacttatg aggaaacaat gcatttcatg
     6781 gatcaaatca atcaattact cggtcgtttt tttatccaag aactggataa tcctgagacg
     6841 atttccgaaa cgattcgcag cttatttcct tattttcctg aaggactagt aacggatgaa
     6901 gaggtggttc atcgccggct gcttaagcga tatgaacaag cacctatcgg tattcctcat
     6961 acaaatatgg ggttattcca tacatccagc tcattgattt ctaaaccgat cttttgtatt
     7021 ttcaatttga aacatccgtt gaccattgaa gggatggata gacagccagt cgatttgtcc
     7081 cgtatgttag ttatgctggc gccttcgcca atccaagaaa aagacgggat tcttttagga
     7141 aagatcagtg gagcaatcat catgaatgac ttgaacaccg agatcttccg ctcaggaaat
     7201 aaagaaatcg tttatcagtt gttagcgaaa atcctgattg aagaaataaa gaaatgaagg
     7261 tgaaaagatg atacagctac cagcagaaaa aatattgctc gatcaatcct atcaaaattg
     7321 gaaagaagcg ttgaccgaga tcggccagtg gatgacccaa caagggggaa tcaaaccggc
     7381 atatatccaa tcaatgatcg aaagacaaga aaaagcgagt gtctatatcg ggaatttcgt
     7441 agccttgcca catgggactg acaaagatga agaagtagta gaagaaggaa tctatttagt
     7501 ccaagtgcct gatggtgtgg actttggaac aaaagaggaa ccaaaaatcg cgacactctt
     7561 atttgtagtg gctttaaaaa aagaacatca gttagcggtg ctccaagatt tagccttttt
     7621 ctgttcagac atcgaccaag taatggcgct cagtgatgcg gaaaccattg aagaagtcca
     7681 aatgattttt gaacatggac atatataacc aatgttcaca gtaaaaaaag ctgccttgca
     7741 tcaagtaata acttcaacca agataaaaat aagaggagct atttttgttg ggaggttctt
     7801 attactgcgg atgcaagctt ttgaaaacta tttattctag aaaaccgttt atttgaaata
     7861 ggggacggtg acaaggtttt tgtcaccgcc cctattattt ttgtgtttta atagctcttt
     7921 tttatataga tagtgcccat acctatgaat aataaaacga tactccatat accaagtgtc
     7981 aagataccat tcatgaaagt aacctgttct gaagctagtg gcgagaaaat atctcctcct
     8041 atgatcacat tttgaaaatc aacataagaa gttggcaagt aatgactgat tgattctatc
     8101 catccaatcg aagaaaacaa gcgatactct gctaagaaaa gacaaagaag tgtacctaaa
     8161 agattgacta gagaatgttc aaataaagca gatagaagaa aactgattgt acttaaaaag
     8221 agcacccaca acaacaggaa cagaaagctc tggaaaagat actcatacat cggcaggatt
     8281 cgtatctcct gaccaatgat cgttcctaca ggataacgga agtctcccaa accatttttg
     8341 attattacta gtatagagac gatcaaaaat ggcatcacga gacttagcat agctgcacca
     8401 taataaatgg taaaacggat gctatgtttc tttaccatac tatccggtaa tagattgact
     8461 agagagatta cctgctttct tttttcataa gagatgatgg aagaaagaaa caaggcggtt
     8521 atacataaaa tcaaaaaact agaaatcatt cctgaaaaaa tattttcgta gtaatttgct
     8581 aaagagagat gcgctggcaa gtcaaaaata gaatatcttt ggatctgatg ttctttcata
     8641 taacggagca actcgacacg tttttgttgt tcaatgattg gaattccttg tctttgacca
     8701 gaaatcagtc gatccagtat atctttttca taatgataag ttgcttcgac catttgctgg
     8761 acattccctg tgttttttgc atgtaagatt gcttcgatca gtttgttgct tttctctaaa
     8821 ctttccacaa attctttctc attctcatct tctttcaacg tttcaatcaa acgttgcatc
     8881 tcttcatagt tatgattcag ttcatcgaat tctgtataag caagtttatg atcaaaaaaa
     8941 agtgggaatg caaagagcac gaccaacaat agaggaaata aaaagtttac tttgtttttt
     9001 agtaagagat tgcgttcaaa tgtaagtaaa gacatagtta cacctcatat aatttacgat
     9061 aaatagctaa aatctcttcc atatttgagg attctgtgac gatttctcca ttttttagaa
     9121 aacatgcttt agaacaaatc gattggatat tcgcgatagc gtgggaagaa agaagaatcg
     9181 tcttcccctc tttctgccat tgtctgatga ggctattcaa gatatcaata cttcctgggt
     9241 ctaatccgtt caacggttca tcgaataaca tgacagatga atcactgatc aagtaagcag
     9301 caagcaagac atgttgtttc attcctagag acatttttcc aatcggcaaa gtaaggtact
     9361 cagtcatctt caatcgttgg aaaacagttt ggcattctac tttgctctgc caatagtatt
     9421 tgatcaaagt caaataatca acagccgata gatagtcgga taactgattc gttgattcta
     9481 gaaaaaagaa agaccggcga taatctgtat aagcagtagg agagatgttg tgaagggaca
     9541 attgatcata agttgttggc agcagttggg caatgccatt cagcaaagtt gttttgccaa
     9601 atccgttcgg ggcaatcaaa gcagctactt ctcctgattc tactaaaaga ttcaattgtc
     9661 gaatcacacg ttttttacca cgtttcatgt tcatgttttt gatcagtaat gtcatagata
     9721 ggcctccaat ataaaattta ataacataat agaatggaaa acatctaaat taaatgctaa
     9781 caaaagttat tgtttgcata ttataaaaaa attaaaataa cagttttatt taaaattgaa
     9841 gttttctgat accgaaatct ttttattttg ctgacatact caaacctttg gcacaaagtt
     9901 tcgtgccaaa gtacgactat ttctcttttt agtaagcgga tacaattaga gtatcaaaag
     9961 gagagaggga gagacaaaat ggaacggacg attcccaaaa acaacatttc gctaaaagcg
    10021 agggtacaaa aattaggcag tactttatca agcatggtca tgccaaatat tggagcactg
    10081 attgcctggg gtgtattaac ggctctattt atcccagatg gttatttacc aaatgaggct
    10141 ttcgctacca tggtaagccc aatgctaact tatctgattc ctttattgat cggttatact
    10201 ggcggaaaag taattgctgg tgaccgcgga tcagttgttg gggcaattgc aacgatgggt
    10261 gtgattgtcg gaacagatat tccaatgatg ctaggcgcga tgatcatggg acctcttggt
    10321 ggatatgcca tcaagaaatt tgatcagcta ttccaaaaac ggatcaaatc cggttttgaa
    10381 atgctagtca ataatttttc agcaggtctt atcggttttg gattagcgtt attaggtttt
    10441 tctgcgattg gtccagttgt cgatgcattg actcaagcaa tggcaaaagg tgtagagatc
    10501 attttatctg cccatttgat tccattgaca agtattttta ttgaaccagc aaaaatctta
    10561 tttttgaata atgcaatcaa tcatgggatt ttgacaccat tgggaactga acaagtagca
    10621 gaggctggaa aatctattct attcttgctt gaagcaaatc ctggtccagg acttggcgta
    10681 ttacttgctt ttactttgtt tggcaaagga gcagcaaaat cttcagcacc aggagccatg
    10741 atcattcact ttttaggcgg gatccatgaa atctattttc cttacgttat gatgaaaccg
    10801 ttattattct tatcagttat aggcggagga atgtcaggaa gtttcatttt ccaattgatg
    10861 gatgctggac taagagcgcc agcttctcca gggtcgatca ttgcgattct agcgatgaca
    10921 ccgttcaatt cttaccttcc tgttattttg ggcgtgattg ttgccgcagc tgtttcgttt
    10981 ggtatttctg cagtaatttt gaaagcggat gcgaaagata caaatgaaga cttcgaaaaa
    11041 tctgttcaag agacaaaggc agctaagcaa gcttccaaag gtgcccaagg tattcctcaa
    11101 ggagctacat tctctggcgt acaaaaaatc atttttgctt gtgatgcagg aatgggatca
    11161 agtgcgatgg gcgcttcgat cttgagaaaa aaagtacagg aagcagactt gcctcaaacg
    11221 gtgaccaatc aagcaatcag ccaactgaca gatgactcga atacattgat cgttactcag
    11281 gtcgaactac aagaaagagc aaaacaaaaa gcaccgagcg cgcgttttgt ctcagtagaa
    11341 aactttttga attcacctcg atatgatgaa atcattcaag aattaagcag caagcagaaa
    11401 acatcagttt ctgaaattga gaatgagcca gcagaagagt ttctttcagc aaacatcaaa
    11461 caaatcaaag agattctact tgtccacgat gatcgttccg gatcagcaac aatggggatg
    11521 accgtgctca aagatatatt agacaagaac catttaacta tcccgttaag aaaagtcaat
    11581 atccaagatt taaaagatga acctgattca ctgatcgtag ccaaagaaag tttagcggaa
    11641 aaagtacaga tggctgcacc tcaatccatt catttatcag tcaacagcat ggtcaatccg
    11701 caaaaatacg aatcgattgc acaatatttg aaacagacag cttaagggag gaaaacagca
    11761 tgcaaagttt gccattcgac atgatcgagc tcaatcgatc ttttaaaacc aaagaagaag
    11821 cgatccgcta ttgcggtcag atgcttgtag aagcagggtg cgttgatgag gaatatatcg
    11881 atgccatggt agatagagac aaaatgttat ctgtttatat ggggaatttt atcgcgattc
    11941 ctcatggaac agatgaagca aaaaaatatg tgaaaaaatc cggcatctgt gtcgttcaag
    12001 taccagatgg tgtagatttt ggtaccgaag aagaagaaaa gattgcgact gttttgttcg
    12061 gtattgcagg agtcggagat gagcatttgc aacttgtaca gcaaatcgca ctttattgca
    12121 gtgatatgga caatgttgtc cagctagcag acgcgctcag caaagaagaa gtaacggaaa
    12181 atctagcaat tgcttaagga gagagaaaaa tgagagcagt acattttggt gcaggaaata
    12241 tcggtcgagg atttattgga gaggttttgg cagctaatgg atttgagatt actttcgtag
    12301 atgtgaatca cacgatcatt gatgctttaa atgaaagaaa agaatacgaa atcgaattag
    12361 cagatgaatc aaaagaaaaa atcactgtaa aaaatgtcaa aggaatcaac aatcaaacaa
    12421 atccagaaaa agtaatccaa gaaatcgctg aagcagattt agttactacc gccattggcc
    12481 cgaatatctt accttttatt gctgagttga ttgcaaaagg tatccaagaa agagaaaagg
    12541 aaggaagcac aacatcttta gatatcattg cctgtgaaaa tatgattggc ggttcagcat
    12601 ttctagaaaa agaagtgtat aaatatcttc cggaaacatc atttactgac aaatatatcg
    12661 gctttccaaa tgcagcagtc gatcgaatcg tcccattaca acaccatgaa gatcctttat
    12721 tcgtccaagt ggaacctttc aaagaatggg tcattgacga tagccaaaga aaaaataaaa
    12781 aaatccaatt aaaaggggtt ttatatgtag atgatctaga accatatatc gaacgcaaac
    12841 tatttagtgt caatactggt catgcgactg tcgcttatac aggtgccttg ttaggttacc
    12901 agaccattga tgaagcaatg caagatgcgt tagttgtggc acaattaaaa tcagttttgc
    12961 aggaaacagg aagtttgctg attgccaaat gggggtttga tgcagagcag catcatgcgt
    13021 atattgaaaa aatcattcat cgcttccaaa acaaaaatat ctctgatgcg atcacacgtg
    13081 tcgctcggac accattacgt aagttaggtt atcaagaaag atttacacgt ccagtgagag
    13141 aactacaaga acataacttg acttgcccac atttgacggc aacaatgggg attattttta
    13201 attactatga tcctgaagat gaacaaagcc gtcaattgca tgaaatgaag atccatgaaa
    13261 acttggaaca attgattcaa gaagtcacag gaatcaatga tccgaaaaca atcgggaata
    13321 tcaagcaaaa cgtgaaccgt tatgccaagc aagtcgctta aaataggata cgaaaaaggg
    13381 gaatgtgacg aaatttttgt cacattcccc tttttcgaat aactaggctg tttgtccttc
    13441 aagatagggc agatccaatt ctttaccgta acgttgttct aaagctaggc ggatcaaacg
    13501 agctgccagg ttctcatttc ttgcaaggat aggtccatgg aaataagaac cgaagacatt
    13561 tcggtaaatg acaccttcag attggtcttc accattattt ccttgacctt gtaccacttt
    13621 ccccaaaggt cgttcacctt cacctaagaa agtccgacca ttgtggtttt caaatccata
    13681 ataagtttca ttgaattctt cgttatggat aacgatatca ccgataaacc ggttattttc
    13741 ctgacttaac gtgtagtgat ccagtgcact gattccttgg atcttttctc cattagctcc
    13801 catgtagtag tgacctagca attggtagcc accacaaatg gctaacatga cgccatcatt
    13861 ttcgatatag ttgatcaaag aatctttctt tttctggatg tcttttgaaa tgattaattg
    13921 ctcaaagtct tgaccaccgc caaagaaaac caagtcatat ttatcagcat caaaaggttc
    13981 atgtatacta acgatctccg tatggcaaga aacccccatt ttttctgcta tatatttcaa
    14041 catcaaaagg tttccgttat caccatatgt gttcatcaaa tttccataaa ggtgggcgat
    14101 attgagttca taattagcca cggttcattc ctcctttgat aaatccttga gcagctagtt
    14161 ctttacgaag ctgcaataca gcagtgtaag tagctaaaat atagacatgc tcagttggca
    14221 attctttgat tttttcgatt acttctggta attcttttgt ttctgtcaat ttattttctg
    14281 gaatcccggc aacttttaga cgtaaagtca tatctgtatg acggtctcct ccagaaataa
    14341 cagctgggat atccatatcg gcaaagtttt caaaattacc atcccaaatc cagctgacat
    14401 cgattccgtc tgcgtaattg gcatttaata aagccactaa agaaaatgga taaggagcta
    14461 gaccaatcat atcgattact tgattgatac ctacaggatt tttgacaagt actaatgtac
    14521 attttttccc attgatattg atgacttctt gtcggccgaa tactttctcg tcatatccta
    14581 aaccttgttt gattttatca ggatcgacat tgaagtactc tgccactgca gtagctgcta
    14641 aagcattgta gacattatac ataccgccaa cttcgattcc gtattcatga ccatcgatca
    14701 cgaaatctgc tgacacattg tccatccgta ccatatcagt taatcgataa tccaattcag
    14761 gacgtttgaa gtcacaattt ggacagtaat atttccctaa gtttgcataa gtgatcattt
    14821 tataatgcaa gatgtgatga caatttggac ataagacacc gtctgtattg taatgagcca
    14881 tttgttcacg atcttcttca tggtcaaagc cataatattt tctaggattg actgtttcga
    14941 tagaattgaa gattggtgaa tcaccgttac tgataatcgt tgcatttggt gactttgcgg
    15001 caccgtctac aatcatttta taggtcgtat agatttctcc ataccgatcc atttgatcac
    15061 ggaaaatatt tgtaaataag aacagttctg gtttcatata ttctgtgact ttgctcaaac
    15121 ttgcttcatc tatttctagg acagcaaact ttttctgtcc tttttttggt tttgcctgca
    15181 agaaagtcga tacaattccc tgtaccatat tcgcaccagt agggttggtt agaacttcat
    15241 cgaattgctg gcgcaaaata ttgactgtta aagcggtagt caatgtttta ccgtttgttc
    15301 cggtgacaac gacgacttgg tagtcttttg ccaaactatc tagtatatgt ggatcgactt
    15361 tcagcgctag tttacctggt aaacttgatc cgcctttaaa aaaagttttt aatgcccatt
    15421 gcgacgtttt tccaactgca atcgcaaaat gactgcgtat tcccattatg taacctccaa
    15481 aaacgatttg ctgacgaata aattcaacta atcataccat atgttgaaaa aaaggttaaa
    15541 ttgcttttct tattcttaag aaaaggttac ggctgaaagt gcatgaaatc ggacaaaatc
    15601 gcatgattta acaaaaaaac agcttgaaac aaaatgattg tttcaagctg ctgtgatttt
    15661 attattccct cttacctata ttcccatttt aaccgataat aattttctcg cttggatatt
    15721 catagcctag atctcgtgtt tcttttggca cgaataacat caatgtatat agcattccga
    15781 tccgcccaat aaacatcagt gtagcaatcg tgattttgcc cacaacagac aagtctccgg
    15841 taatccctaa agataaacca gttgttccaa acgcagaagt tacttcgatg atgatcgaga
    15901 tcagcgtttg ttcttctgta gcagataaaa aaacgataca aaagaaacac ataccaagag
    15961 aaagcataaa gacaacgact gattttcgca catcatcttg atcgattctc cgtccaaaaa
    16021 tattgatgtt atcttcactt ttgaggaagg aatatagata caaaccgatg attgctacag
    16081 tagttgttcg aatcccgccg cctacagaac taggactgca tccgataaac atcagtaaag
    16141 aaaaaatgat cagtgttgtg atttgaaaat cacccaaatc gtgaatctgc agaccagcat
    16201 tacgagtcgt aatcgaataa aacatcgaat tgatccattt gacactcata ttggaatctt
    16261 ggaataaatg gtccttttcc aatagataaa tcaagacagt tcccgaaaca aacaagataa
    16321 caaaagctaa cacagctaat ttggtaaaca aggaaaaacg aaacggaagc ttcgcattgg
    16381 aacgtttgta taatagccat tcccggcact ccatcaatac aggaaaacca attcctccga
    16441 caaaaatcaa aaacatgatc aggatcaaga aaaaatagtc atgtgcaaag ggcttgatgg
    16501 aatccccagt gacgtcaaac ccagagttcg tgacagccga tatcgcctga taaaaaccgt
    16561 aaaaaacggc atcacgccaa cgatcaaaat agcctctgta ataaaaataa atagaaaaga
    16621 atgtcccaaa aagtaattga aaccagatta aaatagcaaa agtgatccga atcagtcgga
    16681 cgataccaga caaacgcggt tgattcatat ccgtcatgat cagctgtcgc tgtttcagtg
    16741 tgatccgtcg tttagaaaaa atgacaaaag cagtagaaat catcatgatc cccaaaccac
    16801 caacttgaaa taatatttcc agcagtataa taccgttatc attgaacaca gaattgatat
    16861 caaaagtcga tagtccagtc acactcactg tactgattgc catgaacagc atgtccacaa
    16921 aagaaacatg gctatctggt tcgcgaaaaa tcggcatata aaatagaacg agtgataaga
    16981 ccgtcatcaa tatataataa aaaacgatga tttggatcga tgagaaatga ttggcagcaa
    17041 acttctgtcc tcgccgctgc agccttcgaa agaataaatc aacatgcaca gtactatcct
    17101 cctgacaata gtcgtttcta ccagtataca aggggaatta ggaatactca aagaaattat
    17161 caaacagata acaatacagc tttcgattaa aatagaaatg gcttataata aggaaaatcc
    17221 tagaaaaaag aaggaggagg aataacaaat tgattaatgc tcaacaaatc gtagaaaaaa
    17281 tgaaaaatca aaaaataaat tatgatacag ttctgaaaaa aatgatcatg caatgggaga
    17341 aagaagaagt aaagcctaag atattaatac atacatgctg tgctccttgc agtacgcaca
    17401 cattggaatt catgtctcaa tatgcagaga tcacgttatt tttttcaaat tcgaatattc
    17461 atcctaaaag tgagtatttg cgaagattac atgaacaaaa acgatttgta gaccaattta
    17521 atgagaaaac tgaccatcat gttgcgctaa tcgttgatga ataccgtcca aatgaattca
    17581 ccaaaatggt tttaacaaac catcttgaaa acgaaaaaga aggtggtgct cgctgcagtg
    17641 catgcttcaa tatgcgatta gatcttgttg cgcaaaaagc gcaagaacta ggatacgatt
    17701 actttggaag tgccctgacc atctcgccaa agaaaaatgc agctttgatc aatcagatcg
    17761 gaatggatat ccaaaagatt tacgggacac aatacttgcc aagtgatttc aagaaaaaca
    17821 aaggctatga acgctctttg caaatgtgca aagagtatga tatttatcgc caatgttatt
    17881 gtggttgcgt gttcgctgcc aagcagcaag gatgcgatct gaaagaaatc aatcgggaag
    17941 caaaagctta cttaaaaaca tcaaccgtag tttttgagga aaattaaaga aatctttcgg
    18001 aaaaacgtaa gaaaaaaata aagaatttat ggtagaaata aggtagactg tataatttgt
    18061 ccgtagtaaa gtaaggaaac aagaataaat ttcaatgaag gaagagataa gatgcgaaaa
    18121 gtagcaccta ttttgaagcc aatcgctgta ttaacaatct cgacgattgt cgcgactact
    18181 acaggtattg tgaatagttc cagctaccgt gcaccaagtt caagttctag tactgtagaa
    18241 gaaacaacac caactacttc agtctcgaca tctgaaccaa caacaacgga aagtttgaat
    18301 gaaaccacta cgacaagtac atgggacgat acaacaacta gttcgtcgta tgatgagaca
    18361 tatacaactg acagctcaag cgaaacaagt aatacggtac cagatggcac tccatcggat
    18421 tcaaccaatc caagtacaag tccggataca agtgctagct ccgaaacgat accaagcagt
    18481 acgactgtag atgataacac gcaaaatgat gatgcagcac gtacagcaca gcaaaaccaa
    18541 gattcaggaa atacccaaac gtataaccag caagcaggtg attctcaatg acatttttac
    18601 tagaccttat caacggggtg cataaattcc ttggctattt agacatcagt cctaaatatc
    18661 ttaaccgtgg atatacgatt ttaagtgtga ttcccacgct ttatttattg agaattgttt
    18721 atggactttg gcaaaaccaa aactatcttc aatttttctt atatggactt gcatttctag
    18781 tgcttgttta ttttactgtt ttaaatgtat tttattactt tttagataaa aacacgaaag
    18841 ccgatgtcac tcagcttttt gtgaagtatt tacctgatga agcgttcaac atccagacag
    18901 aagaatcgat agataatgga aattcgatcg ataaagtgaa tacacaagag gtcatggttt
    18961 cttatgacga agattatcaa ttgaaattag ccgagaatat gcggtatttg attggaaatg
    19021 gtgagatcaa aacaaatgaa ctcggtcgca tggatggatt tctggtcgat agaaatacat
    19081 tgtatcctta ttactatgtc aagaaaacag gagagaaaga gtatgttcta aatattggac
    19141 gaagctactc ggacatggaa aaagtaggga ccattcgact tgactcaaca gaagacacat
    19201 tggaacctgt cggattgttc atcgtcggcg gtgattttgt caaagacggg ttacgctatc
    19261 atgagcctta tcgtctgaaa ttgattgcaa aaaaggcaca gtcgcaggaa gaaccagtgg
    19321 tttatagtcg tagccaaaga agaagacatt cataatgtga tgaaacgccg tgtatcagtg
    19381 gttgcgataa aaacgtttat tcggaataag ggggatgtga cacgtctttt gtcacgttcc
    19441 ttttttatat aacgagaaag ataagacgaa cacatcactt attctttcct ttcatttata
    19501 aacattgtag aataaagaaa gtacaacgaa gtattgtgaa aaaacgtaat aaataacgag
    19561 gaaaatacaa gtaggaggta agctgtgaaa gaatttttga aaaattgggg gattttgatc
    19621 cttgtattag cggctatttt attagctaga gtgtacgtgt ttacaccagt tactgtaaac
    19681 gggcattcga tggaccctac attaagtgat ggacaacgat tgatttcatc taagatctca
    19741 aactatgaac gaatggatat tattactaca aaggaaccgg gggatgaaga acggatgatt
    19801 gttaaacgga tcatcggtat gcctggagat acagtcaaga tggaaaatga ccagttgacc
    19861 attaatggca aaaagtatga tgaaccttat ctggatgagt tcaaaaaaga attttcagaa
    19921 gataagttac aaggagaata tgcttatagc tcaggattcc aagcacaagc agaaagttca
    19981 tccaccttta cgagtgactt tgaatatact gtgccaaaag gaaagtatct tgttctaggc
    20041 gataaccgct tgatttcaaa agacagccga atgtttgggt tagtcgacaa agatatgatc
    20101 caaggaaaag ttgttttccg ttattggcct ctttcagaaa ttaaaatcat gtgatgaaaa
    20161 aataaaaaga agctgtgaca tgttttttgt cacagcttct ttttccgaaa attccttctt
    20221 atttcttgga gttgctcact ttttttgcaa tctcatttta agctttttgg ctgatggata
    20281 agaaaagcga agcgtttttg ttcataaata gcttttcccc acgatggaag gtatgactag
    20341 tagcagagac aaagcccgtt ttttccaatc gattattcaa ttcttcttgg ctaaagccat
    20401 tatggacttt cggatgacta atttgttcat tcttgtcaaa gtctacgatg atcaattgac
    20461 cgccaggagc taaaatttca taaagttttg taagaatatt ctccgtatca ggaatgtgta
    20521 aaagtacaag tgatagaagg atgagattag cttggacagc aggaaattcg acagaagcat
    20581 cagcgtggat cgttcggaca tttttcagat ctgctgcttg gatcttttct tctgccattt
    20641 tcagcatagt ttctgaagca tcggcaatga tcagttcttt gaatctttcc gcaagcggta
    20701 aactaacaag cccagttccg cctccgtaat ccagcaggac tttttggtgg ctgtcgcgcg
    20761 gtagataagt agttagttct tcgcgaataa tcttggctaa tgcttgacgg tcaggggaat
    20821 cataatggtt agcgatggaa tcaaaaatat tttttgtcat caaatagctc cttacgtaaa
    20881 actttgaaat tttaacttat tatagcacgt gaaaagaaag aaagcatgct atactcaatt
    20941 caaaaaggga gaatctagaa agaatatgcc gatatcgttt gtaaaagata gagaggaaaa
    21001 aggaaagtgt gtccgagaaa tattactaga cttgcctgag tggtttgggc ttcctgaatc
    21061 aacggaaaaa tatatcgagg aatcaagtaa actaccttta tggtgtgaaa aaagaaaaga
    21121 agaatattta ggctttatca ctctttcaca gacaagtgaa gatactgccg aaatctattc
    21181 tattgtatgg gaataaaaaa agactatcat caccaaggat taggaacaaa actttttaaa
    21241 gaagcagaag gttacgcagc aaaacattac aaatatttgc aagtgaagac tgtagacgag
    21301 gggcactact ctatatatga tcaaactatt tgtttctacg aatcattagg attttcaagg
    21361 ttggaagttt ttccaaattt atgggatgaa tggaacccat gtttagttct tgtcaaaaaa
    21421 ttggaacaaa agtgagcaat agatttaata ctaatctgtg cttgatgaaa attctcactg
    21481 taaaaaatgc tgaaaatcaa gtataatacc ataggaacaa actggaaggt gaaaaagagc
    21541 atggcacaac tattttttaa gtatggcgca atgaatagcg gcaagacgat cgagatccta
    21601 aaagtcgcac ataattatga agaacaaaat aagccagttg tcttaatgac aagcggtatt
    21661 gatacaagag atggtttagg cgttgtttca agccgaatcg gactgaaacg taatgcgatg
    21721 ccgatatttg aagaaacaga cgtatttgac gtaattcaga aaatggaaag caaaccctat
    21781 tgtgtgctga tcgatgaagc gcaattctta aaaaagaaac atgtcattgc ttttacgaga
    21841 attgtggacg aattggatat tccggttatg gcatttggtt tgaaaaatga ttttcgaaac
    21901 gagttatttg aaggatcaaa atatttattg ctttatgcag acaaaatcga agaaatgaaa
    21961 acaatctgtt ggttctgcca caaaaaagca atcatgaatt tacattatat tgatggaaac
    22021 cctgtttatg aaggcgatca agtgcaaatt ggcggaaatg aagcttatta ccccgtttgt
    22081 cgaaaacatt actttcaccc atcaatgatt acagaaggag aataagcatg tacgatcaat
    22141 tacaagcaat tgaagaccgt tatgaagagc ttggagaact tttaagtgac ccagaagtga
    22201 tttctgatac aaagcgtttt atgcagcttt caaaagaaga agcaaacacg cgcgaaacag
    22261 tcgaagttta tcgtcgttat aaagaagtag tacaaggaat caccgataca gaagaactgc
    22321 ttggtgaaaa actggatgat gaaatggctg aactagcaaa agaagaatta tctgaattaa
    22381 aaaaagaaaa agaagtctta gaagaaaaaa tcaagatctt gcttttacca aaagatccaa
    22441 atgacgataa aaatatcatc atggaaatcc gtggagcagc aggcggagac gaagcagcat
    22501 tattcgctgg cgatcttttc aatatgtacc aaaagtatgc tgaagcacaa ggctggaaaa
    22561 cagaagtgat ggaagctaac gttaccggaa tcggtggata taaagaagtg atcatgatga
    22621 tcaccggaga caacgtatat tcgaaattaa aatatgaaag tggtgcgcac cgcgtccagc
    22681 gtgttccttc aactgaatcg caagggcgta tccatacttc caccgcaaca gttgtcgtga
    22741 tgccagaagc tgaagaagta gagatcgata ttgaagacaa agatatccgt acagatatct
    22801 atcatgcttc aggtgctggc ggacagcacg tcaataaaac agcatctgct gtacgtttga
    22861 ctcacttacc aacagggatc gttgttgcta tgcaagatga acgttctcaa atcaagaacc
    22921 gtgaaaaagc aatgaaaatc ttgcgtgctc gtgtgtacga taagatccag caagaagcac
    22981 aaagcgagta tgatgccaat cgaaaatcag cagtcggaac tggggatcgt tcagaacgga
    23041 tccggacgta taatttcccg caaaatcgtg tgactgatca tcgtatcgga ttaactatcc
    23101 aaaaattaga tcagatttta gcagggaaat tagatgagat cgttgatgca ttgatcatgt
    23161 atgatcaaac atcaaaactg gaagctatgc aaaatgggta aaatgaccta tcgagaagtc
    23221 ctgtcacggg cttcttcttt tttagaggat catgggaaag aagcatatag tatccaattt
    23281 ttatttttgg aacgaaaaca atggaaaaaa cttgattggc tccttcatat gaacgaagag
    23341 atttcagaag aagaacagcg gttgatcgaa accgatctgc gacttctgct tgcagatcat
    23401 ccacctcagt atttgctagg atatgcagat ttttatgatc atcggttaaa ggtaactgaa
    23461 gcaacactga tccctcgacc agaaacagag gagttagtcg aatggtgttt agacgagacg
    23521 cctggtgtac cgttagaagt gatagatatt gggacaggga ctggagcaat tgctattagt
    23581 ttgaaagcag caagaaaaaa ttggcatata tctgctgtgg atctttcaga agaagcatta
    23641 gaagtagcga aagaaaatgc tcagaaagaa ggaacgaaaa tcagttttta tcatggagat
    23701 acattaggac cagtaatgga tcagcaattt gatgtgatta tttctaatcc gccgtacatc
    23761 agtcggaatg aatgggaact gatggatgaa agtgtccggt cattcgaacc taaaatggcc
    23821 ctttttgctg agaacgatgg attagctatt tatgaaaaaa tagcagttga agcaccaagc
    23881 gtactaaaat caaatggaaa gattttttta gaaatcggat ttcgccaagg tgaagcagta
    23941 aaaaaaatat ttcagcaagc atttccagat aaaaaagtgg cgataaaaaa ggatcttttt
    24001 ggcaatgaac gaatgatcca agtttatggt tgaagttgtg gatgaaaaat tttttatttg
    24061 tttttttatt ataaaaaaac acttatttga caaggatgta aacgactttt tggtttatca
    24121 acaagtttat ccactattga caaatagatt atccacaaac agtggaaaac ttgaggataa
    24181 actcagacaa aataagaatg tatgaaaaag gggttgtgga taaactgtgg aaacgaaacg
    24241 atatgatgaa acagaattgc aagaggcagc cgaagcactg aaagaaggag aacttgttgc
    24301 ttttcctacc gaaacagtat acggattggg cgccaacgct cttttaccaa acgctgtcaa
    24361 gaaagtgttt tctgtaaaag ggagaccgca agataatcca ttaatcgtcc atgtcgcctc
    24421 ttttgaacaa gtaaaagagt atgtggacaa ctttcatccg gcagcagaga agattgtgaa
    24481 gaacttctgg cctggaccat taactttgat atttaagatc aaaaaagaca ctttgccctc
    24541 agttgtgacg ggaggattat ccacagctgc atttcgtata ccggataaca aaaaaacact
    24601 agaagtaatc gaactttccg gtgttccgct tgtcggtcca agtgcaaata cgtcgggaaa
    24661 accaagtcca acgactgcag atcatgtata tcatgatcta caaggaaaaa ttaccgggat
    24721 tattgatgat ggagctacgc gaattggtgt agaatccact gttcttgatt taagtgatcc
    24781 aacagccatg cctatgattt tacgaccagg tgcagtaacg aaagaacaaa tcgaagcagt
    24841 catcgaatct cctgttgcta ttgatcagca tcttgtaaaa gaaaatgaaa caccaaaagc
    24901 ccctgggatg aaatacaaac actactcacc cgatacacgt gttctgatgg tcagagatgg
    24961 cgattggtca actgcagttc aatgggcaaa aaataagaag atccgtgcag gtgtcatagc
    25021 aagccccgaa attgcggatc aagtgcggac agatactgca gctgtttata tgtataacga
    25081 caactcagta gaagcggcgg ctaaaggatt gtttgcaggt ctaagaggat tagatgaacc
    25141 aacacttgga ttagatttga tttttgtaca agtgtatcca gaaactggat tggggaatgc
    25201 ttacatgaat cgcttgaaaa aagcagcagg acaaaattat tttgaaaaat gatcaattgg
    25261 ttattgtcgc attcttttag tatgatacaa tgctatcaag taaatcttct atcagtgaac
    25321 gaaaaactaa gcgtaactaa tcgtaataga agagatacga atatttggca atttgttttt
    25381 gatgggaaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg tagattacaa
    25441 aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac aagaacataa
    25501 tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg cacaaggaag
    25561 tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg gtgggtgtga
    25621 atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat tcggagcgaa
    25681 atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt atctcgcttt
    25741 ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac atctaacaca
    25801 tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc
    25861 gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac atcaaccaaa
    25921 attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga aattcagaga
    25981 aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg caggattagt
    26041 agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga ctaccactca
    26101 taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag cattagcaaa
    26161 aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc atgtgattgc
    26221 gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat atagtgaaca
    26281 aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg gaaccagagt
    26341 catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt taggtataaa
    26401 tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata aaaactcgat
    26461 tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca cgccagcaat
    26521 cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc
    26581 attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg tacgcgaatt
    26641 aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt aaacagaaaa
    26701 gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga caatatcggt
    26761 gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa
    26821 gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga tgcaaatgcg
    26881 cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact agaagaagta
    26941 caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt ggatactggc
    27001 gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga taaattacca
    27061 gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac ccatgataaa
    27121 ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga atatagtcga
    27181 tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg
    27241 aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc aggagattca
    27301 gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa gggcctccca
    27361 atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac agattcctat
    27421 catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa
    27481 tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag agcaaatata
    27541 aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga tgtattaaga
    27601 gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt tacacaagta
    27661 cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca gacaaaggca
    27721 acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata agctttgctt
    27781 gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc gagtttgact
    27841 agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa ggagagctta
    27901 aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac taactattat
    27961 tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa tcgctatgtt
    28021 gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg aaacaccgat
    28081 cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc caatcttgcg
    28141 cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta aagtgggaca
    28201 tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg tcaaacttcc
    28261 agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa caggcggatc
    28321 agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta aatttgtttg
    28381 tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg atattgacat
    28441 ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc ctggactagg
    28501 ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg gtgtgacaca
    28561 a
//
LOCUS       DAYYNG010000019        28503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000019 DAYYNG010000000
VERSION     DAYYNG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.019"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(290..1087)
                     /locus_tag="ACHBFA_000695"
     CDS             complement(290..1087)
                     /locus_tag="ACHBFA_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM9394210.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="ACHBFA_000696"
     CDS             complement(1084..2268)
                     /locus_tag="ACHBFA_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM9394211.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="ACHBFA_000697"
     CDS             complement(2293..3540)
                     /locus_tag="ACHBFA_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9394212.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="ACHBFA_000698"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="ACHBFA_000698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9394213.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="ACHBFA_000699"
     CDS             5520..5834
                     /locus_tag="ACHBFA_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9394214.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="ACHBFA_000700"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="ACHBFA_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9394215.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /gene="tmk"
                     /locus_tag="ACHBFA_000701"
     CDS             6519..7163
                     /gene="tmk"
                     /locus_tag="ACHBFA_000701"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9394216.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="ACHBFA_000702"
     CDS             7178..7507
                     /locus_tag="ACHBFA_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM9394217.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="ACHBFA_000703"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="ACHBFA_000703"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9394218.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="ACHBFA_000704"
     CDS             8495..9319
                     /locus_tag="ACHBFA_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM9394219.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="ACHBFA_000705"
     CDS             9312..9659
                     /locus_tag="ACHBFA_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9394220.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="ACHBFA_000706"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="ACHBFA_000706"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9394221.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="ACHBFA_000707"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="ACHBFA_000707"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9394222.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="ACHBFA_000708"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="ACHBFA_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9394223.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="ACHBFA_000709"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="ACHBFA_000709"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9394224.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="ACHBFA_000710"
     CDS             15316..16083
                     /locus_tag="ACHBFA_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394225.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="ACHBFA_000711"
     CDS             16073..18115
                     /locus_tag="ACHBFA_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9394226.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18387..18536
                     /locus_tag="ACHBFA_000712"
     CDS             18387..18536
                     /locus_tag="ACHBFA_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9394227.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18547..20061
                     /gene="dltA"
                     /locus_tag="ACHBFA_000713"
     CDS             18547..20061
                     /gene="dltA"
                     /locus_tag="ACHBFA_000713"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9394228.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20058..21263
                     /gene="dltB"
                     /locus_tag="ACHBFA_000714"
     CDS             20058..21263
                     /gene="dltB"
                     /locus_tag="ACHBFA_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9394229.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21308..21541
                     /gene="dltC"
                     /locus_tag="ACHBFA_000715"
     CDS             21308..21541
                     /gene="dltC"
                     /locus_tag="ACHBFA_000715"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9394230.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21544..22812
                     /gene="dltD"
                     /locus_tag="ACHBFA_000716"
     CDS             21544..22812
                     /gene="dltD"
                     /locus_tag="ACHBFA_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9394231.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22953..24035
                     /locus_tag="ACHBFA_000717"
     CDS             22953..24035
                     /locus_tag="ACHBFA_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9394232.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24035..24838
                     /locus_tag="ACHBFA_000718"
     CDS             24035..24838
                     /locus_tag="ACHBFA_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9394233.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24908..25648
                     /locus_tag="ACHBFA_000719"
     CDS             24908..25648
                     /locus_tag="ACHBFA_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9394234.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25659..26663
                     /locus_tag="ACHBFA_000720"
     CDS             25659..26663
                     /locus_tag="ACHBFA_000720"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9394235.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26708..28501)
                     /locus_tag="ACHBFA_000721"
     CDS             complement(26708..28501)
                     /locus_tag="ACHBFA_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394236.1"
                     /translation="MKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYT
                     KDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKIL
                     YQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLIS
                     FIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSE
                     DILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVL
                     LYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHC
                     LIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLL
                     EQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCS
                     FCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETH
                     AIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDV
                     ADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8982 a         5047 c         5872 g         8602 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttaa aagtacgaga aattataaag
    18241 aaaaaagcag tgatttgatg tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa
    18301 aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt
    18361 gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt
    18421 attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt
    18481 atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg
    18541 aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct
    18601 gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca
    18661 gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt
    18721 ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc
    18781 tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct
    18841 aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc
    18901 ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat
    18961 agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca
    19021 aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac
    19081 tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg
    19141 gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca
    19201 gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg
    19261 gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat
    19321 gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa
    19381 agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca
    19441 actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta
    19501 ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt
    19561 tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg
    19621 aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat
    19681 acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat
    19741 cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc
    19801 caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag
    19861 cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact
    19921 aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc
    19981 cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca
    20041 gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt
    20101 tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa
    20161 gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta
    20221 attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat
    20281 ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta
    20341 ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag
    20401 catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca
    20461 tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc
    20521 tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc
    20581 agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata
    20641 tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc
    20701 ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata
    20761 tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg
    20821 gtacaagcta catgatggga tatcagacac caatcaattt caataaacct tttttatcat
    20881 ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt
    20941 atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg
    21001 taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga
    21061 cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat
    21121 ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct
    21181 tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt
    21241 tagataaact tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa
    21301 aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt
    21361 aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt
    21421 tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga
    21481 gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg
    21541 agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg
    21601 tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt
    21661 gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta
    21721 tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt
    21781 catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca
    21841 cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa
    21901 aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact
    21961 gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag
    22021 aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa
    22081 gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct
    22141 gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc
    22201 agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca
    22261 aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg
    22321 acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag
    22381 ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat
    22441 tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca
    22501 ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt
    22561 gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc
    22621 aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg
    22681 ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag
    22741 tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta
    22801 caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt
    22861 ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat
    22921 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
    22981 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
    23041 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
    23101 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
    23161 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
    23221 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
    23281 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
    23341 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
    23401 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
    23461 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
    23521 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
    23581 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
    23641 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
    23701 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
    23761 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
    23821 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
    23881 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
    23941 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
    24001 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
    24061 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
    24121 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
    24181 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
    24241 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
    24301 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
    24361 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
    24421 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
    24481 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
    24541 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
    24601 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
    24661 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
    24721 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
    24781 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
    24841 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
    24901 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
    24961 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
    25021 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
    25081 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
    25141 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
    25201 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
    25261 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
    25321 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
    25381 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
    25441 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
    25501 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
    25561 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
    25621 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
    25681 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
    25741 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
    25801 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
    25861 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
    25921 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
    25981 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
    26041 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
    26101 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
    26161 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
    26221 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
    26281 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
    26341 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
    26401 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
    26461 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
    26521 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
    26581 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
    26641 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
    26701 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
    26761 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
    26821 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
    26881 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
    26941 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
    27001 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
    27061 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
    27121 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
    27181 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
    27241 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
    27301 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
    27361 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
    27421 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
    27481 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
    27541 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
    27601 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
    27661 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
    27721 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
    27781 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
    27841 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
    27901 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
    27961 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
    28021 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
    28081 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
    28141 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
    28201 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
    28261 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
    28321 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa
    28381 cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa
    28441 agaaataagc aattcctgtt aaaaataaca atgataatat aatgattatg ctccacttta
    28501 tac
//
LOCUS       DAYYNG010000020        27724 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000020 DAYYNG010000000
VERSION     DAYYNG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.020"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            293..2059
                     /locus_tag="ACHBFA_000722"
     CDS             293..2059
                     /locus_tag="ACHBFA_000722"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM9394237.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2161..3420
                     /locus_tag="ACHBFA_000723"
     CDS             2161..3420
                     /locus_tag="ACHBFA_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9394238.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3567..4868
                     /locus_tag="ACHBFA_000724"
     CDS             3567..4868
                     /locus_tag="ACHBFA_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394239.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4871..5722
                     /locus_tag="ACHBFA_000725"
     CDS             4871..5722
                     /locus_tag="ACHBFA_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9394240.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5735..6550
                     /locus_tag="ACHBFA_000726"
     CDS             5735..6550
                     /locus_tag="ACHBFA_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM9394241.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6701..7867
                     /locus_tag="ACHBFA_000727"
     CDS             6701..7867
                     /locus_tag="ACHBFA_000727"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9394242.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8097..8744
                     /locus_tag="ACHBFA_000728"
     CDS             8097..8744
                     /locus_tag="ACHBFA_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9394243.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8828..9772
                     /locus_tag="ACHBFA_000729"
     CDS             8828..9772
                     /locus_tag="ACHBFA_000729"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9394244.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9907..11358
                     /locus_tag="ACHBFA_000730"
     CDS             9907..11358
                     /locus_tag="ACHBFA_000730"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM9394245.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11379..12215
                     /locus_tag="ACHBFA_000731"
     CDS             11379..12215
                     /locus_tag="ACHBFA_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9394246.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12215..12874
                     /locus_tag="ACHBFA_000732"
     CDS             12215..12874
                     /locus_tag="ACHBFA_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9394247.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            13000..13689
                     /locus_tag="ACHBFA_000733"
     CDS             13000..13689
                     /locus_tag="ACHBFA_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394248.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13695..14651
                     /locus_tag="ACHBFA_000734"
     CDS             13695..14651
                     /locus_tag="ACHBFA_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM9394249.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14695..15207
                     /locus_tag="ACHBFA_000735"
     CDS             14695..15207
                     /locus_tag="ACHBFA_000735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9394250.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15463..16011)
                     /locus_tag="ACHBFA_000736"
     CDS             complement(15463..16011)
                     /locus_tag="ACHBFA_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9394251.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16100..17281)
                     /locus_tag="ACHBFA_000737"
     CDS             complement(16100..17281)
                     /locus_tag="ACHBFA_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM9394252.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17517..19025)
                     /locus_tag="ACHBFA_000738"
     CDS             complement(17517..19025)
                     /locus_tag="ACHBFA_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9394253.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19184..19375
                     /locus_tag="ACHBFA_000739"
     CDS             19184..19375
                     /locus_tag="ACHBFA_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394254.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19578..20495
                     /locus_tag="ACHBFA_000740"
     CDS             19578..20495
                     /locus_tag="ACHBFA_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM9394255.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20488..21561
                     /locus_tag="ACHBFA_000741"
     CDS             20488..21561
                     /locus_tag="ACHBFA_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394256.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21709..22227
                     /locus_tag="ACHBFA_000742"
     CDS             21709..22227
                     /locus_tag="ACHBFA_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394257.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22208..22600
                     /locus_tag="ACHBFA_000743"
     CDS             22208..22600
                     /locus_tag="ACHBFA_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM9394258.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22676..23611
                     /locus_tag="ACHBFA_000744"
     CDS             22676..23611
                     /locus_tag="ACHBFA_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9394259.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23744..24523
                     /locus_tag="ACHBFA_000745"
     CDS             23744..24523
                     /locus_tag="ACHBFA_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9394260.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24516..24833
                     /locus_tag="ACHBFA_000746"
     CDS             24516..24833
                     /locus_tag="ACHBFA_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9394261.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25077..26687
                     /locus_tag="ACHBFA_000747"
     CDS             25077..26687
                     /locus_tag="ACHBFA_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9394262.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26844..27692
                     /locus_tag="ACHBFA_000748"
     CDS             26844..27692
                     /locus_tag="ACHBFA_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9394263.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8979 a         4898 c         5557 g         8290 t
ORIGIN      
        1 ctaaattatt atgtttcatt cttctagaat atacttctac gttaaaaact gttaaaaaac
       61 ttttacttta gaaaattgga gaaattgatc gcttaagata aattcaggac actaaatagt
      121 acaggaagta gattaaaact tttaaaagtt ttaagtctat ctatttggca agtttaaaaa
      181 ataaaaattc ggtatcaaaa aactgttacc gataacattt ttgaaagcgt tgtcttttgt
      241 ttctctatca cttatagtga tgacgtcata ttaaaacaca aggaggaaat caatggatac
      301 agctgcaatt tatcatcgac cggaaagtga atatgcattt ctatatacga atacaagatt
      361 tcgtattcgg cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc tttgtggaga
      421 tccatatacc ataacaactg aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct
      481 aagtaccaat gttcatgatt attgggaaat tgaagtgacg agcgaaacaa ggcgcttaca
      541 gtatgcgttc catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat
      601 ttttccttat cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt
      661 tcatcaaatc gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat
      721 ttttcccgaa agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg
      781 gggaagtaaa gatccagacc gagaagactt cttcggggga gatttgcaag gtgtgaggga
      841 tcatttggat tacttgacgg accttggcgt caatggcatc tatttttgtc ccatatttaa
      901 agccacatcc aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg
      961 cgataaaaaa cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat
     1021 gttcgatgct gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa
     1081 aaacggagaa aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc
     1141 ttatcagatg actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt
     1201 tactccattc atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga
     1261 tatcgcaact tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa
     1321 tgaagtcgat catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga
     1381 catctatatc cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt
     1441 ccatgcagtg atgaattatg cattcacaga ttcgattaaa gattattttg caaaaaagaa
     1501 aatcacagct tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca
     1561 ggtgaacgaa ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct
     1621 ttgccaaggt aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg
     1681 gtctccttgt atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg
     1741 ccgcaaatgt atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa
     1801 agaacttgtt tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg
     1861 gctaatagta tatgatcgtg aagataagct ctatttcaca cgggaactag agggacagat
     1921 catctatgtt tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt
     1981 gattcttagt caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg
     2041 atttttgatc atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa
     2101 gatttgtgcc cattataata agggtgtaac agcacgttga atacacagga ggaaatgaag
     2161 atgaaaaaaa gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc
     2221 ggattagcgg cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa
     2281 acgttaacag tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact
     2341 ttcgaaaaag aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg
     2401 gaatcattag cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat
     2461 gaccggatcg gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa
     2521 gcagcttacg atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa
     2581 cctgcagtaa tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca
     2641 tcaacgttta aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa
     2701 gcaggaaaaa atacaggatt tttagctaaa tggactgact tttattattc ctatggttta
     2761 attgcaggtt acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt
     2821 ttgaataatg caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt
     2881 tggccaaagg gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg
     2941 tccaataaaa ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac
     3001 aatatcaatt atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg
     3061 tttggcggag gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa
     3121 aaatggcttg attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat
     3181 gaaatccctg caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca
     3241 acagctgtga ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc
     3301 gaagtttgga caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca
     3361 aaagaatccg cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga
     3421 ttaaaatgat aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc
     3481 aagagcgaga tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt
     3541 ttagcaagtt tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa
     3601 aaaaaatcac gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt
     3661 ttaaaggtat cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg
     3721 gatttcatgc attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat
     3781 tatttatttt gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt
     3841 acggtttgaa tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa
     3901 tcaatcggac gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat
     3961 tgacacttcc cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat
     4021 tgttcactgc atttactaat tatgacttca atcatattcc accagccagt ttaattgatt
     4081 gggtaggggc taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt
     4141 ttgcagtctt ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc
     4201 ttggtattct cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg
     4261 gtatcatttt cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga
     4321 atatattcaa cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc
     4381 ttccttttgt agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag
     4441 caattatctt agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa
     4501 tcttacaatc gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat
     4561 ggcagcgctt ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta
     4621 ttacacagta tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggtg
     4681 gtcccggaag cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata
     4741 aacttacgac tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct
     4801 ccgtcatcgt tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg
     4861 gggattaaca atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt
     4921 ttttacttac ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac
     4981 agcaagtact gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga
     5041 ctggacttta ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa
     5101 aaatacattg atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc
     5161 gggctatact tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt
     5221 agtgattcag atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt
     5281 attagaagcg ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc
     5341 aatgaatact tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc
     5401 tgctaaactt gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt
     5461 caaaccgatg ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat
     5521 gttagctaag ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac
     5581 atttatttct gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat
     5641 tgcactaccg atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac
     5701 atctggcgga acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt
     5761 ttccgcatta cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa
     5821 atttttcttg atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta
     5881 tgccaatcag atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga
     5941 tgaacaagga acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc
     6001 gcaacaaacg atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca
     6061 acaatccgat catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa
     6121 ggataaatct ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc
     6181 tgtacgtacg cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg
     6241 tccaacaatc attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag
     6301 tctcatttta ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc
     6361 cattcataca ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat
     6421 ggcagcagca gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac
     6481 aacgataact gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg
     6541 tgtcgaatga caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa
     6601 agtagttttt gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta
     6661 gtattaacgt aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata
     6721 caatcaatta ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac
     6781 aacgaaacaa tacggagata cggacactcc tccccaaatc aaaatcaata tccatgaaga
     6841 aatcccaatg aaagagatta caagtaacgc ttctttggca ctaggagaag cctatatgga
     6901 tcatcgaatc gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc
     6961 aaacagcttt ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac
     7021 gaaacaaaaa aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa
     7081 actttggcta gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac
     7141 gttggaacaa gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga
     7201 aggcgaaacg ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa
     7261 agagtataat gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac
     7321 caaaagaata aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg
     7381 cgaactaaaa ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg
     7441 ttctgaaaat cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac
     7501 agctttgatc catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa
     7561 atatattttc cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa
     7621 aaacgatttg caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga
     7681 acattggaca aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc
     7741 cttctatcga atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa
     7801 tatcgatgtg attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg
     7861 tgcataattg aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga
     7921 tgctccagaa gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt
     7981 tttctttata ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt
     8041 ttttcttcgt tatacttgta atagaatact attttataac aaaggggatc gaatcaatga
     8101 aaaaaactgt tatttatact agtttatttg cagctttgtt tttagctgct tgtcaatcaa
     8161 ataccaatca aggcacatct gggaatacgt cttccagtag cgaacagcgc tcttcacagg
     8221 tatcggactc ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa agttctagtg
     8281 aagcaagtac tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag
     8341 ataaataccc agatactgca attactagtt tggaattaga tagtgattgg ggatcttact
     8401 tctacaagat agaaggtgtc gatgatcaaa aagaatacga agcaagaatt gatgctcaaa
     8461 atgaaaaaat ggagacagag aatccagaaa ctctagatcg agatgaacaa aatggtcaaa
     8521 agaaaaaaga agatggtctg gatgttgata aattgatcac gattgaggaa gcagcaaaaa
     8581 tcgccgtcga caaagtagga agcggtacgg caacagattg ggatttggat aaagatttag
     8641 gtactactta ttgggaagtc aaagtaaaag acggcaatca aagtacatca gtcaagatca
     8701 atagccaaac aggcgaagta ctgtcaactg aacaagatga ttaagaattt cttcggaaaa
     8761 caagagttga tttttctttt agtcatgata cactagacaa aataacttgt ttgaaggagc
     8821 gatttacttg agtttagaaa cgacctttgc caatcatatc tttgccaatc ctttgatgaa
     8881 tgcatctggt gtccactgca tgactaccca ggaattagat gaactggctc attcagaagc
     8941 aggagcattt atcactaaga gctgtacgat caatgaacga aaaggaaatc ccgaaccacg
     9001 ctattttgat gttcctttag ggagtatcaa ttctatggga cttccaaact taggtttttc
     9061 ttattatcta gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt
     9121 atttttttcc attgccggga tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga
     9181 aaaaagtggg tttaatggga tcactgaatt aaatttatct tgtcccaatg tgcctggaaa
     9241 accacaacta gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag tattcagcat
     9301 cttttctaag ccgctaggaa tcaaattgcc tccttatttt gattttgctc attttgatca
     9361 gatggctgat atcttgaatc agtttccatt gacctatgtc aatgctatca acagtgtggg
     9421 caatggtttg tatatcgata cagaacaaga agcagtagtc atcaaaccaa aagaagggtt
     9481 cggcggcatt ggcggagagt atatcaagcc gactgctttg gcgaatgttc gcgcttttta
     9541 tacacgtttg aaaccagaaa tccaaatcat tggtacaggt gggattcgga caggacaaga
     9601 tgcatttgaa catctgctat gtggcgcaag catgttacag attggtacag agcttcataa
     9661 agaagggccg gaaatttttt ctagaatcat aaaagaatta acgcagatta tgtcagaaaa
     9721 aggatatacg tcaattgacg aatttaaagg caaattacgc accatttcat agcaaagaga
     9781 cgaaggtcat gattgatctt cgtctttttt tataaaaaac actcgtctgt tcattcctgt
     9841 gcaagcgtac tcattgtatg ttagacgaac ttgtgggaaa ataacagagg caaaaaggag
     9901 gataatatga atgatgcaac agaattagca aaagagctta aagaaaaaga atttagtttt
     9961 caagaactag ttacggatat acagaaaaaa ataaagaagc aaaatcatga attgaatgcg
    10021 tttgtcgcgc ttgaaccatt cgagagtaat tcccacgagc tattgattcc ccagtcagaa
    10081 aaagggccat tggctggtat cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga
    10141 tggctagcta cttcaggatc tcgtttattt gaaacacacc gagcttctca tacatctaat
    10201 tatgtgcggc aagcagaagc gataggtcta gttccttttg gacaaaccaa tgcgccagag
    10261 tttggtttca aaaatattac agacccagtc atctatggcc cagcaagaaa cccttggaac
    10321 cttgaatgta caccgggcgg atcaagtgga ggagctgctg ctgcagtttc atcaggaatc
    10381 gttcctttag ctggtgctag tgatggcggt ggttctatta gaatacccgc ttcgttttgc
    10441 ggattgattg ggctgaaacc ttcgcgaggg acgatgccag ttggtccata tgcttggaga
    10501 ggatggcaag gagctgctat tgatttcggc ttgacagttt ctatgagaga cacggaggct
    10561 ttattttatg ggatgagaag tatccattcc ggtgcgccat atcaagtatc gccagttgag
    10621 tggcaaacac atccaagaaa aaaacggttg aagatagctt tttgtgttga ttcaccgatc
    10681 cactcacaga ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga tttccttgga
    10741 aaagaagggc atgatttgac agagattcct tacccactag atggtcgtaa actgatcacg
    10801 tcttattatc agatgaatgg cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc
    10861 atccagcgag ctgtcagaaa agaagaactt gaacccattt cttggacttt gttccaatat
    10921 ggtcagaagc tctctgccgc tacttacatc gattctctgc atgtatggga tgaagcagca
    10981 attgtcatgg aaaacctaca tcaagaatac gatctattcc tttcaccaac gactgcattt
    11041 accgccccta aaatcagcga agatttgcaa agtgatgcga taagagcaca gatggaacac
    11101 atcagtgatt gttctgaagt agaaggttta gacgttgttt cttccatgtt tgaggagagc
    11161 ttgaagatca caccttatac acagttggct aatctgactg gacaaccagc aatcagcttg
    11221 cctactcatt tgacaaaaga aaacatgcct ctaggaatcc aatttatggc tgccagagga
    11281 agagaagatt tgctatttca aatcggctat ctatttgaag caaatcatca gttccattta
    11341 ccaaatccat ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa agattgtttg
    11401 ctctaataac tagtgccttt tttattttaa gcgcttgcgg tacagctgcc gctaatcagg
    11461 aagataacag ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg acttctggca
    11521 cactgtttcc cgcatcttat tataatgatc aaaatgaatt agttggttat gatgtagatt
    11581 tggcaaaaga ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg
    11641 atggacaaat cacttctgtt tcaaaaagag aatcagattt tgctgcgaac gattttggta
    11701 tcacaaaaga aagagaaaaa aaattctccc tttcagagac tttcaaatat tcatttgaca
    11761 gtatgatcgt acgaaaaaaa gatgattcag gaatccactc acttgaagat cttaaaggga
    11821 aaaaagcagc tggcgaaccc aatactagct atatgcgttt agcggaaaaa cacggagcta
    11881 aattagtcac atatgacaat gccacgaatg atcagtattt aagcgatgta gcaaatggtc
    11941 gaacagatgt tattctaaat gattattatc tgcagaaaat ggccgtatct gctttaccag
    12001 atatccctgt aaaaatactg gagaacgttt atttcaatga aaacgaaagt ggttttttat
    12061 tgaaagaagg aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag
    12121 aagatgggac gacaaagaaa atatctgaaa aatattttca aactgatgtt tcagtcaaac
    12181 cgaaagaaac gatggctgag tcggtgagtg agtgatgtac tttcctctaa ttgatttttc
    12241 cctcatgtgg acttcacttc ctttcgtact gcaaggcttg gtatacacat tagggattgg
    12301 cttcgtgtcg tttcttttag gaaatttggt agggttgctt ttgacagttt taggtctttt
    12361 ggattggctg cctctgaatg tatttattcg tttttattta tcgttcttta gaggcattcc
    12421 cgcactagtc ctgctgtttc tgttatattt cgggttacct tatcaattaa gtgccttaac
    12481 ggcttcagtc atctgcttta cgattaccag cagtgcattt attggtgaaa tctatcgagg
    12541 atcactagca ggcgtttctt caggtcagtg ggacgctggt ttagcttctg gtttttcttt
    12601 ctttggggta ttgaaatata tcgttttgcc gcaagctttt cgaatctcag taccggcact
    12661 cagtaacgta gctatggatc tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc
    12721 agaaatattt caaaaagcaa agatcatagg cggtcggaca ttcgattatt tttctctcta
    12781 tattttagtt gcactgattt attgggtcat ttgtatcggg atcgaaaaag tgcaacagta
    12841 tttagaaaaa cgttctatcc atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct
    12901 ggcaccattt ctattttttg tttcttgtat attctgccta gtattttaaa accatttcat
    12961 gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca tgaagacccc gctaaccatc
    13021 gaaatagaat cctcgttata ccaatattgc atcgagcaag gcggcttggt tgtagaagaa
    13081 gtcacgatgc ctgatgacca aggaattgta gatacgctta gctgcttgac aaaatttgat
    13141 ggaagccgag aatggcgatg ctatgagctg aaagtgacaa aagcagattt tcgctctaca
    13201 gccaaactgt catttgttgg acattataat tattttgttt taccaagtaa actatatgaa
    13261 gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc gtacacggtt
    13321 gaagaggaga tgcctgcctc tggtacattt ctgattgcga aaaaagcagc aaagaaagag
    13381 cttgctgtac cggaagaagc tttgacaaac cgcttcatgg cttctttatt cagggaagta
    13441 agaaaagcaa agcaaatgga gcgaggcgta aaatattttc caaaagatca attgtacaaa
    13501 gaattgaaaa gacgttcaga aaatcaagat ccattttcta acgaacgata ttatgaacgg
    13561 tttttagagg aaatagctag tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt
    13621 cagcatgatt atgaatattt gaggcaacaa cagcagatcc gtcggttgcc tacagagcca
    13681 ttggagtgaa aaatatgtac tatccttatt taagaggaaa acaatttgac ctattagcat
    13741 taaaagaagc actgtcgcga ggattgctct ccaataaaat ccaaccagtg atcgaaccag
    13801 tacgtgattc cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag caccatccaa
    13861 tcgctgtgat ccaaaatccc caagtaggac aattcaaatt attcgatcag catgtgcaca
    13921 gttggacagt caaagaaaat agctctgtcg tccctgcaca gatcttaacg ccagaaaatc
    13981 tcagtgaagt actggattca cctccagcgt ttcttgtttt cgatggacag cactacccaa
    14041 aagatacaga ggtgtggaaa cagttggcag gagttgattc gaaatttttg ataccagata
    14101 ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa
    14161 cgagaaagca tgttgaaagt tatgcagata aaacagatga cttttttagt gatgactatc
    14221 tttttttcca cgcagacgga tatattggat tttctgattt cacgatcgaa ggtagccgtt
    14281 atttcgataa agggttccct agccgagcgc ttgctatcca tctaacgtat attgatgctt
    14341 acggcaatat acgcgtgaaa cattttgtct ctgacagtaa cgactctgct aaagaccaag
    14401 cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc
    14461 aactcttgat tacttcagga atgattgaat tattggctct ttaccagcaa caaaaattcc
    14521 ctggactggg agtgttgaaa aagtggtcac ttatgcatca tttggaactt attagtcacc
    14581 tgctagatca tccaaaagat tggcttaaat attattcaaa agttcaggaa ctatatgaag
    14641 tgatccaatg attagataat gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat
    14701 attcggcaaa caaggaaaga agaagtagaa gaagtggtag aaatcattaa tgccgcagcg
    14761 gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa
    14821 acgattttga acgatattga aagaaaagaa agctatgtat taatagtgga agatcaaatt
    14881 gctggaacag ctgcattagt ctcaggcgtg gatcccgttt acacaaaaat agatggtagt
    14941 tggaaagaat caactggtcc ttatttatcg attcatcgtg tcgctttagc agaaagtact
    15001 cgaggaaaaa aactagcaaa accgttctta gaacatttgt tgacgattgc agccagcaaa
    15061 aatatccaag atatccgtat cgatacttat ccagctaatg aaccgatgga aaagaccatc
    15121 tattctgctg gattcactta tcaagggatg attgaatttc cttttgaaca cggcgaaaga
    15181 aaagcatatc aaataatcat caattgacta taaaatcatc tgagcaaaga taaagacatt
    15241 aacactactg aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa gtggccagct
    15301 tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta
    15361 ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac atagcttttg
    15421 tcacgatctc tcggcttatc ctacgagtat tattcgatac atttatatca ataatccttt
    15481 ttttatttta tctgcagctt cttggaacgc tccatcgttt gacacgacta aaagtgaggg
    15541 gatagctttc aaacgtttca actgtgctaa atcttctaca taaaccgcca gtctggctga
    15601 aatctgttca gatgaatctc cacgtttttt caggcgcgat tgcaatgttt ctttagagat
    15661 cgtaataaag ataggacgga ctaccgtccc gaaatactca tataaatgcc aaaaaccatt
    15721 tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc tttttaattt ctgctttact
    15781 ggaaccatat ttatgttgag catattggtc ccactctgca aactcatttc ccttgatcat
    15841 ctgattgaat gtattgtcag aaacaaaata ataatcctct tgatcttttt ccccaggtct
    15901 tggtgatcga ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag cttctgcgag
    15961 tgaagttttt ccagaaccag aaggaccgat aaatacatag caataattca ttgtcatttc
    16021 tcctttgatg attgaacaag tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt
    16081 aactttgctt ttttgtattt tataccagca agccagtcac aatagcactt aaaaagctga
    16141 ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc gacaagattt cctttttctt
    16201 catttaatcc cttcatcgca ccagaaatga tcccgataga agagaagttg gcaaatgaaa
    16261 cgaggaagac agatataatt gctgttgtac gctcagtaaa atggaaatta ccatttgcta
    16321 attctgtcat agcaacaaat tcatttgtaa ccagtttagt cgccatgata ctaccggcat
    16381 ccactgcttc tttccatggg attccgctga tgaaggcaag aggggcaaag atgtaaccta
    16441 ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat agcattgatc atggcaatca
    16501 aagcaacaaa accgataagc atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa
    16561 tgtattctcc caacatttgg aaaaatgtct gttctttgtt ttcttcaatg atcagctcat
    16621 cttctttttc ttctaataca tagggattta caattgaagc cagaatataa ccgccaaata
    16681 agttcaaaac aagggcagtg atcacatatt tagaatcgat cagtgccata taagatccga
    16741 cgatcgacat agaaactgta gacatagccg atacactcat ggtgaacaaa cgtttctcag
    16801 ataggaaggg taattccttt tttatggaaa taaaaacttc cgattgtcct aaaatagcag
    16861 aagctactcc attataagat tccaatttgc ccatcccgtt gattttactt aatcctagac
    16921 caacagcttt cataaataat ggaagaatct tgatatagcg tagaataccg ataatagcag
    16981 aaataaatac aattggcatc aatacggaaa gaaagaaagg tgcacttcct ttatttgcta
    17041 caccggcaaa aacaaagtca acaccttgcc cagcaaaagc aagaagatga tcaaacattt
    17101 ttgccaatac agaaacaacc gctgtaccaa aagttgtacg taataatacg aaaccaagaa
    17161 caagctgtaa aacgaacatc acaattagtg gtttcaattt gattgctttt ttgttactgc
    17221 tgatcaaaaa agcaagccct aatgtgacaa ataaaccaat aatacctaat aaataactca
    17281 aataatcact cctattccaa attccgtttt attttacctg ttttgcaaat aaaatactaa
    17341 tctttagtga aattttcatt acttttttag agtgaaacgc attcattttc cattagaaaa
    17401 taaagcgaac aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa
    17461 cgaaactatc gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat
    17521 tggtaatata gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt
    17581 ttcaatgttt gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca
    17641 agttcagcta gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat
    17701 aaagcgatca agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat
    17761 ttcaatcctt gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata
    17821 tgggctaaat gttttactgc atttaagata actgccgctg acgcatccat caatgaactt
    17881 gtacgcccgg taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct
    17941 tttgtaggac tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt
    18001 cggtcttcag gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct
    18061 tcgtcgatca atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct
    18121 tgtttagagg cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca
    18181 cccatgtcag taggagattg ataaacagat tctttaccag taattttttc aataatacgt
    18241 ttgattacag gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca
    18301 tcaaagtgga aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa
    18361 gcaatgttca gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag
    18421 taaccagctg catgtccttt ttggctttca tgatacagtt ggttcaagca agtagcgagc
    18481 tttccacttc ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac
    18541 tcattttttc caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg
    18601 atttctgagt gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc
    18661 gtggcaggct gtccactata gcgagtgata acgacactgt tgattaataa gccataacca
    18721 cgtaattcat cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg
    18781 atcttattac gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc
    18841 aatttttgca gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca
    18901 tgcttgtcgt caactaactt cccaccaaat tctaaatata atttgtcata gtggttcact
    18961 cgttccaaaa tatacgctga ctgctcttca atatattttt ctgaatcaaa accaattttt
    19021 ttcactaggt tccctcctta agtactaaag cattttttca tttttttgag gattcgtcaa
    19081 ctggaagagc gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat
    19141 agttattcat tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa
    19201 aaagaaaaac gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct
    19261 tgaagatccc gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac
    19321 tcgtgatgag catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat
    19381 aagataaagg tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa
    19441 aaaaggctgt accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta
    19501 taacaatttt ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt
    19561 aagaggaggc aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac
    19621 tagaggagac gaacgcggat caggatacag gacaagaatc gtcttctttt tacgatatca
    19681 aagaaaaagc aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag
    19741 ctgtccgcga agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag
    19801 aattaaaaag aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc
    19861 atgatcgttt tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga
    19921 tgagctatta caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa
    19981 gtctgccaga aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac
    20041 cggagtcaac ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc
    20101 agacagaaat tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt
    20161 tgaacgacaa taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag
    20221 agaagaagaa gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc
    20281 ctgaaacaat ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc
    20341 gtgcagccat cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg
    20401 gattcggtca agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata
    20461 atcaatctaa tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa
    20521 aatgaagaac aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt
    20581 aatcctctgg atgacacaga agaaatccct atgctagagg aaacaaaaat acatgaaaaa
    20641 gaaaaagtaa ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact
    20701 gaaaatcccg ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt
    20761 gaaacagcgg aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat
    20821 tttccacaat tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg
    20881 atcggaaaga caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat
    20941 attggtctgg aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc
    21001 aattatacgt tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat
    21061 caacgatacc aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc
    21121 aatgtccaag atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc
    21181 tctgcagggc agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga
    21241 acacttggtt ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat
    21301 cttcagagcg aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg
    21361 aaaaatccat cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat
    21421 aaaggacagt ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca
    21481 tctggagact acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca
    21541 ttaatggtga gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc
    21601 tgttccaaag gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta
    21661 ctaaatggac gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat
    21721 aaaatagcac agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa
    21781 tttattccga atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc
    21841 ttaaaagatg cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt
    21901 gttggaccat gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca
    21961 atcgtctgct tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt
    22021 ctggcgggca tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg
    22081 cctcaacctt ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc
    22141 aatgttgtgt tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga
    22201 ggaaaaaatg aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt
    22261 gacaagttgc agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac
    22321 atcgcaaaaa gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt
    22381 ggtcttcaaa aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga
    22441 agataacggc ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta
    22501 ttggatgttt actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa
    22561 tgatggacaa tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa
    22621 agggcgaata ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga
    22681 aaattttaaa aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt
    22741 tgctatcagc aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag
    22801 catccacagt atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc
    22861 caataaagga cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag
    22921 tacaatcgtc gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt
    22981 tgaaaagttg acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact
    23041 gttatttggt ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga
    23101 attgaatact gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat
    23161 actgattatt tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg
    23221 aacaatattg acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat
    23281 cggtttcttg ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat
    23341 cggtgaaagc gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga
    23401 gatcagtcgg ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc
    23461 agcaaaagca gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat
    23521 tgaaaaaaat atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga
    23581 taaatatgac ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat
    23641 gagaaaaata taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca
    23701 atgagtccgc tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag
    23761 tgcgccatgg cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt
    23821 aggattagct tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact
    23881 gttatcgatt ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac
    23941 gcaagcatca tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt
    24001 attaggagca agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt
    24061 ttctcaatca ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc
    24121 ttcctggaat aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg
    24181 tagcaatatg atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca
    24241 ttttgctttg acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact
    24301 gatctttaca ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa
    24361 tactttcgga ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa
    24421 ggcattcaaa aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc
    24481 gctcttcaac ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt
    24541 tagttgcttg tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta
    24601 ctcatcgaaa agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat
    24661 ttgcggcgtt agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga
    24721 atatcaaaat agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg
    24781 cgttgtcagt tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt
    24841 tattttatta aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca
    24901 tttgcaacag tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg
    24961 cttatgatgc aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt
    25021 ttttgctata atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga
    25081 ataaaaaatt gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt
    25141 taggggtagt ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc
    25201 ttgctacgac attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta
    25261 cagtcggatt tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata
    25321 acgctgcttg tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg
    25381 cctctgctgc actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta
    25441 acgttaataa tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa
    25501 ttcttagtgc aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt
    25561 ctttgattat cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc
    25621 gcgtattgac tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct
    25681 tcggtgggtt agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg
    25741 aacttaaaga ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg
    25801 tctctatcat tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc
    25861 aaatgatcga ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag
    25921 atcagcaact ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga
    25981 tcctgatctc catggtagga acagtagcta taagcagctt gccaatatta agcacgatga
    26041 agaaaaaaga tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat
    26101 tgattcttct tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt
    26161 tttttggcta tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt
    26221 ttttctcttt attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg
    26281 cgatccgatt aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag
    26341 gattagttgg cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg
    26401 ttcgcggcta tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca
    26461 attttttctt aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt
    26521 tctttttgct gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg
    26581 tggctgtagg aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt
    26641 ttcgaatgtt gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt
    26701 tttttgctgt cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt
    26761 ccaattttag tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc
    26821 tttgaaacga ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc
    26881 tgccggaacg tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg
    26941 atgggaaaat ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa
    27001 aaaacagtaa agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat
    27061 atgaggaatt gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga
    27121 tttctggctt tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca
    27181 ttccttatga ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag
    27241 acttaaatga tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag
    27301 ataataccta tgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt
    27361 tgacaaatgg tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt
    27421 ctgaagggaa aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag
    27481 ctgaagaaat tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga
    27541 tacatgcaaa taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc
    27601 caaatgcaga aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa
    27661 aagccatcgg cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa
    27721 atat
//
LOCUS       DAYYNG010000021        27545 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000021 DAYYNG010000000
VERSION     DAYYNG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.021"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..1052)
                     /locus_tag="ACHBFA_000749"
     CDS             complement(108..1052)
                     /locus_tag="ACHBFA_000749"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9394264.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1348..2292)
                     /locus_tag="ACHBFA_000750"
     CDS             complement(1348..2292)
                     /locus_tag="ACHBFA_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9394265.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2513..3727
                     /locus_tag="ACHBFA_000751"
     CDS             2513..3727
                     /locus_tag="ACHBFA_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9394266.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3749..5764
                     /locus_tag="ACHBFA_000752"
     CDS             3749..5764
                     /locus_tag="ACHBFA_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9394267.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5847..7220)
                     /locus_tag="ACHBFA_000753"
     CDS             complement(5847..7220)
                     /locus_tag="ACHBFA_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9394268.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7246..7530)
                     /locus_tag="ACHBFA_000754"
     CDS             complement(7246..7530)
                     /locus_tag="ACHBFA_000754"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9394269.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7532..9547)
                     /locus_tag="ACHBFA_000755"
     CDS             complement(7532..9547)
                     /locus_tag="ACHBFA_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9394270.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9726..10031)
                     /locus_tag="ACHBFA_000756"
     CDS             complement(9726..10031)
                     /locus_tag="ACHBFA_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM9394271.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10120..11151)
                     /locus_tag="ACHBFA_000757"
     CDS             complement(10120..11151)
                     /locus_tag="ACHBFA_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9394272.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11358..12233
                     /locus_tag="ACHBFA_000758"
     CDS             11358..12233
                     /locus_tag="ACHBFA_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9394273.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12385..13227)
                     /locus_tag="ACHBFA_000759"
     CDS             complement(12385..13227)
                     /locus_tag="ACHBFA_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9394274.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13319..15196)
                     /locus_tag="ACHBFA_000760"
     CDS             complement(13319..15196)
                     /locus_tag="ACHBFA_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9394275.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15193..16614)
                     /locus_tag="ACHBFA_000761"
     CDS             complement(15193..16614)
                     /locus_tag="ACHBFA_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9394276.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16617..20006)
                     /locus_tag="ACHBFA_000762"
     CDS             complement(16617..20006)
                     /locus_tag="ACHBFA_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9394277.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20263..21654
                     /locus_tag="ACHBFA_000763"
     CDS             20263..21654
                     /locus_tag="ACHBFA_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394278.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21727..22374)
                     /locus_tag="ACHBFA_000764"
     CDS             complement(21727..22374)
                     /locus_tag="ACHBFA_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9394279.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22382..23068)
                     /locus_tag="ACHBFA_000765"
     CDS             complement(22382..23068)
                     /locus_tag="ACHBFA_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394280.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23074..24147)
                     /locus_tag="ACHBFA_000766"
     CDS             complement(23074..24147)
                     /locus_tag="ACHBFA_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9394281.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24369..25043
                     /locus_tag="ACHBFA_000767"
     CDS             24369..25043
                     /locus_tag="ACHBFA_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9394282.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25048..26388
                     /locus_tag="ACHBFA_000768"
     CDS             25048..26388
                     /locus_tag="ACHBFA_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9394283.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26537..27523)
                     /locus_tag="ACHBFA_000769"
     CDS             complement(26537..27523)
                     /locus_tag="ACHBFA_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM9394284.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8018 a         5218 c         5013 g         9296 t
ORIGIN      
        1 atatgaatca ttgtatttgc cgttgcggaa ggatttttta attaaccaca gataaacatt
       61 gatgctgtta agcagtttta ggatttttag ttcatattca ttgtttctta taatacagtg
      121 ttcaatactt ctcttaaagc acttgttgat ttgctgaaca ttgctttttc ttcttcgttg
      181 attgataatt caacaacttc acgaacacca ttttcgtcaa cgattgatgg cacacctgta
      241 aagatatctt tttcgtcgta ttcaccagtt aaataagcag atacaggcaa tacagcttgt
      301 tcgttattca agatagcttt tacgatacgt gcagtactca taccaatacc atagtaagtt
      361 gcttttttac gatcaatgat ctcataagca gcattacgga ctttatcagc aatcacatct
      421 aattcatctt gggcaatacg atggtctttt tctacaattt caaacactgg tttaccaccg
      481 acagttgtat gtgaccatgc agcaacttct gaatcaccat gttcacctaa gatatagccg
      541 tggacgctgc gtgggtcaac ttttaatttc aatgcgattt ctttacggaa acgagttgta
      601 tccaatgttg ttccagtacc gataacacgt gatgttggca gaccagattc gttccaagcg
      661 atatatgtca aaatatctac tgggtttgaa gcaacaacga taataccgtc gaatcctgat
      721 cccatgattt cttttacgat ttggcgcatg attgacgcat ttgttttaac taaatctaaa
      781 cgagattgac caggtttttg gttgatacca gctgtgataa caacgatatc agcatcttta
      841 cattcttcat agccacctga ccatacagcc acattttcat cgccccaagc cataccatct
      901 aaaaggtcca atgcttcccc ttctgctttt tcttgattca catcgatcaa tactaattca
      961 ttagagattc cttggttgat cattgcataa gcgatacttg ttccaacaaa acctgtaccg
     1021 acgattacta ctttacgact tgtttttttc atgaataaac agctcctttt cataactatt
     1081 tgttactttc atgaaaagta tagtcttgtt tattcatttt atcaacatgt taattataat
     1141 agttgttatt ataatttttg atggaaacga attcctgaat acgcataaca gataaatgac
     1201 aaatgtttac acctatatcc cctgataata caaccaaaag tttagaactg aaaataaatc
     1261 caaaaaaatt cgctgcaacg atataataag acagcaatcg cacctaaaac aattttcctt
     1321 catatgcttt agtgaaagat tttcttctta atgtttattt ctccattcat tgggagtaca
     1381 tgttgaatac ttcttgaata atttgaataa agaagtcgtt tgtttatatc cgatacgatt
     1441 agcgatttct tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt
     1501 tttctttaga accagttctt gaaaggaagc atcaaaatac attttcaatt tattactaag
     1561 atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag aagtaaaatg
     1621 cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat ctgctgatga
     1681 aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg tatagagatt
     1741 taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat tttcaatcaa
     1801 atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat gttccccttt
     1861 ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt
     1921 caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa tatcctcttg
     1981 ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt cactttcttc
     2041 catctctatc ctttctccat taataatttg taaacatgta ccgctataca tataattgat
     2101 ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt tactgatatg
     2161 aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct gaagatggta
     2221 agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt cgattgattc
     2281 ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa ttgactccct cctttttgta
     2341 agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa gctatttttg
     2401 gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc tattgagaga
     2461 ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga acatgcttat
     2521 gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga cttctgctgg
     2581 tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt ctttatctgc
     2641 tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt tgattagtag
     2701 ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag tgattgcatc
     2761 gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag cttcaagagc
     2821 tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga ttgctgcctt
     2881 ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc aaggatttgg
     2941 tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc aatatacctt
     3001 tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg ttcctacacc
     3061 tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa tcaaaacgaa
     3121 tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat ataatgctgt
     3181 atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc
     3241 gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt ttggctggct
     3301 tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg gaatcggctt
     3361 tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga tcctaacagg
     3421 aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc aagcgcccaa
     3481 gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag gtactttcat
     3541 tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa cacttgctgt
     3601 tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc tagtagtaca
     3661 caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac aattgacaac
     3721 aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag atagatggct
     3781 ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc aacgggaaga
     3841 acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga acgtcaagtt
     3901 taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc aaggagttgg
     3961 gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt gtccagctgg
     4021 taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga tgtttatcga
     4081 gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg aagaatatcc
     4141 agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa tctattatca
     4201 aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct tattcgattt
     4261 tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata tcgaatctat
     4321 cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt gctactataa
     4381 gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat
     4441 accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt tatctctttc
     4501 gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt cagtatcaga
     4561 agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg
     4621 gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt tttttgacga
     4681 ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca agcagcatat
     4741 gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc actatatgat
     4801 gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat taatgagata
     4861 cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag attggatatt
     4921 tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag tcttattact
     4981 gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat atggagagaa
     5041 cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac aaaacctatt
     5101 tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat ctgggagaag
     5161 aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta acaaagacca
     5221 acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga ttactactcc
     5281 atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga atatcgtcta
     5341 ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa ctttctggga
     5401 agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag atccttatgg
     5461 aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac gctattttat
     5521 cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta atctagattc
     5581 atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc ttgaaaaaac
     5641 aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa tgaatggtaa
     5701 aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa aaataaatgc
     5761 ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc tgatattgtc
     5821 caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat gtttgttctt
     5881 tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat gattccaact
     5941 gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg tactagataa
     6001 tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg aaggataaat
     6061 aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc ccctcttcta
     6121 ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg tcctactacc
     6181 ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag tgttcctaca
     6241 aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa aacgatagga
     6301 acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg gaatgctgga
     6361 acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc agctgtgaat
     6421 gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag ttctctttca
     6481 acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat caagagcatg
     6541 atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat gtcctctgta
     6601 gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata accgatattt
     6661 ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa cggttgacaa
     6721 agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa gatcatccac
     6781 aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga aacataaagc
     6841 attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag taaaatattc
     6901 acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg caaagcaatc
     6961 gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt ttggaacatc
     7021 gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag tactaggaat
     7081 ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt ctgaagaacg
     7141 agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat tgacacaaac
     7201 aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt cttttgacaa
     7261 gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca aaggtacgag
     7321 cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga agaaataatc
     7381 agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact cgtccacatc
     7441 tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac ttgttcctaa
     7501 tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt atgatcgcta
     7561 atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta ttagaaagta
     7621 gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca gctaaacaga
     7681 tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt tgaagtttca
     7741 gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt aatgcgatgt
     7801 gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata gctttgatat
     7861 aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg attgcttctt
     7921 gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat tcagatagca
     7981 taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt aatacttgat
     8041 agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc gccgacaaca
     8101 attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt acttgattga
     8161 tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga gactttttct
     8221 tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt tcgtcaacct
     8281 cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat aattgctgaa
     8341 gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc acggctctta
     8401 tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat agtatagtga
     8461 aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac ggaagtaatg
     8521 cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg atcatcacat
     8581 taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga taaaagacat
     8641 tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc atgcgatgga
     8701 tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca tctccttcaa
     8761 cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt tcggaatagc
     8821 caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc aacagttcag
     8881 catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa ctcaaataat
     8941 aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa atcgttttat
     9001 ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt aaatacgtga
     9061 gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt gcttctcttc
     9121 ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac gacagctgac
     9181 aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact gtccctttag
     9241 aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca
     9301 tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat cgagtaatat
     9361 ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata cgggtatcat
     9421 ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt acttcgatga
     9481 tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa ctaattttgt
     9541 agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt ttaaatcgag
     9601 gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac gatttctatc
     9661 gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga attgatttcg
     9721 tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt ttctttgaca
     9781 tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg ctttgtttcc
     9841 tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc accagagttt
     9901 gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc tgctaaagcc
     9961 acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca
    10021 taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac aataagacag
    10081 tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc cttctgattc
    10141 ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt cttcatcttt
    10201 gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga cacggctgta
    10261 atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat ctatataggt
    10321 caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa
    10381 cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc
    10441 gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta tcgaaatttt
    10501 catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt tccctaaaag
    10561 ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa cttgatctac
    10621 tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat gcgtattttc
    10681 tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat cccctaattt
    10741 tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta
    10801 acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg cttcctttga
    10861 cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg ccatttttcc
    10921 atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct caggatcatc
    10981 ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc acactggttc
    11041 agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg aaaaaggcaa
    11101 atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc
    11161 ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct
    11221 attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt
    11281 caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag
    11341 aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca
    11401 cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga
    11461 cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca
    11521 aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc
    11581 aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata
    11641 gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac
    11701 aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc
    11761 gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa
    11821 tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg
    11881 cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc
    11941 attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg
    12001 gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg
    12061 aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa
    12121 atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa
    12181 acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga
    12241 tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa
    12301 acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag
    12361 atatattata gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa
    12421 aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa
    12481 tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac
    12541 ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag
    12601 tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt
    12661 cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc
    12721 acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct
    12781 ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga
    12841 ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat
    12901 ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc
    12961 aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg
    13021 ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac
    13081 cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct
    13141 aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc
    13201 tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata
    13261 aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact
    13321 aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc
    13381 ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt
    13441 tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga
    13501 tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca
    13561 gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa
    13621 aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg
    13681 ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg
    13741 tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat
    13801 cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa
    13861 atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac
    13921 cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt
    13981 gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat aaggcataag
    14041 catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt
    14101 tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga
    14161 cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg
    14221 ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat
    14281 ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag
    14341 cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt
    14401 tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga
    14461 aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga
    14521 tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta
    14581 gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg
    14641 tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat
    14701 ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca
    14761 ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag
    14821 gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag
    14881 gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg
    14941 cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt
    15001 gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca
    15061 ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc
    15121 cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt
    15181 ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa
    15241 gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg
    15301 aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt
    15361 atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc
    15421 agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa
    15481 agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta
    15541 tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc
    15601 agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata
    15661 tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat
    15721 ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt
    15781 tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt
    15841 gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg
    15901 taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat
    15961 atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt
    16021 tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt
    16081 aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac
    16141 taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata
    16201 cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc
    16261 gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt
    16321 tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga
    16381 atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa
    16441 agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa
    16501 tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc
    16561 tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag
    16621 ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc
    16681 cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc
    16741 tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca
    16801 tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt aactggctct
    16861 tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt
    16921 aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt
    16981 tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg
    17041 tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt
    17101 gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca
    17161 taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta
    17221 ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt
    17281 ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt
    17341 atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa
    17401 ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg
    17461 gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc
    17521 ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt
    17581 tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct
    17641 gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt
    17701 ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga
    17761 gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga
    17821 ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat
    17881 ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga
    17941 atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta
    18001 acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga
    18061 agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca
    18121 atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc
    18181 tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa
    18241 tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta
    18301 ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg
    18361 attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct
    18421 aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca
    18481 tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc
    18541 ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc
    18601 atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg
    18661 tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta
    18721 gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag
    18781 gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg
    18841 gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt
    18901 gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca
    18961 ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct
    19021 gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg
    19081 atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc
    19141 gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta
    19201 cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat
    19261 tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat accatcccat
    19321 tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc
    19381 caactggctt taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga
    19441 ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa
    19501 ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct
    19561 gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc
    19621 ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt
    19681 ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca
    19741 tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct
    19801 atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac
    19861 tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct
    19921 gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact
    19981 ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac
    20041 gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta ttttaatatg
    20101 ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc
    20161 atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga
    20221 gaaaaacata tgatcaggtt gttggaatta aactatagaa agatggaaaa aatcattcga
    20281 attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt
    20341 tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta
    20401 acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat
    20461 ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt
    20521 attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct
    20581 tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg
    20641 atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt
    20701 gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa
    20761 aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa
    20821 agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc
    20881 caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat
    20941 aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata
    21001 aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat
    21061 gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc
    21121 aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt
    21181 attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg
    21241 caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact
    21301 ctactgaatc agaatctgat ttattattta tctagctata tcttttggat gcttatcggt
    21361 aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt
    21421 cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt
    21481 cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat
    21541 gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat
    21601 gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc
    21661 aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt
    21721 ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa
    21781 ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat
    21841 ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct
    21901 taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt
    21961 tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg
    22021 atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt
    22081 ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata
    22141 caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat
    22201 cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag
    22261 taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg
    22321 catccacatc tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga
    22381 ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat
    22441 cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat
    22501 gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg
    22561 ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc
    22621 tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta
    22681 aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa
    22741 ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa
    22801 atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt
    22861 gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga
    22921 aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat
    22981 aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa
    23041 cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc
    23101 tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat
    23161 cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat
    23221 cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa
    23281 ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac
    23341 tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc
    23401 aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc
    23461 aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc
    23521 tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga
    23581 agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac
    23641 gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta
    23701 actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat
    23761 gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc
    23821 ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct
    23881 gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa
    23941 ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca
    24001 atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa
    24061 tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata
    24121 gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga
    24181 caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt
    24241 ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa
    24301 aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag
    24361 gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa
    24421 aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt
    24481 tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga
    24541 atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt
    24601 tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt
    24661 atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac
    24721 gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa
    24781 aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat
    24841 tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc
    24901 ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact
    24961 tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg
    25021 gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt
    25081 cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt
    25141 actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata
    25201 aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt
    25261 ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca
    25321 tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat
    25381 aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat
    25441 ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc
    25501 cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta
    25561 gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt
    25621 atgattgttt tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg
    25681 cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa
    25741 ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat
    25801 catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc
    25861 actggattga cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt
    25921 tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag
    25981 atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca
    26041 attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat
    26101 acgaatccag aagataagat cattttggat tcatctgtta accataaaga atggctgatt
    26161 cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga
    26221 ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca
    26281 attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca
    26341 caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta
    26401 aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc
    26461 cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa
    26521 cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg
    26581 tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt
    26641 cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct
    26701 cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag
    26761 attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta
    26821 ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat
    26881 gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt
    26941 tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca
    27001 tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc
    27061 cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg
    27121 ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc
    27181 cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat
    27241 cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt
    27301 tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta
    27361 taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga
    27421 tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc
    27481 tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca
    27541 aatag
//
LOCUS       DAYYNG010000022        26813 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000022 DAYYNG010000000
VERSION     DAYYNG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.022"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1087
                     /locus_tag="ACHBFA_000770"
     CDS             17..1087
                     /locus_tag="ACHBFA_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9394285.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1102..1770
                     /locus_tag="ACHBFA_000771"
     CDS             1102..1770
                     /locus_tag="ACHBFA_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394286.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1790..2368
                     /locus_tag="ACHBFA_000772"
     CDS             1790..2368
                     /locus_tag="ACHBFA_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9394287.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2769..3680
                     /locus_tag="ACHBFA_000773"
     CDS             2769..3680
                     /locus_tag="ACHBFA_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9394288.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4058..4975)
                     /gene="rnhC"
                     /locus_tag="ACHBFA_000774"
     CDS             complement(4058..4975)
                     /gene="rnhC"
                     /locus_tag="ACHBFA_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9394289.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5177..5614
                     /gene="zapA"
                     /locus_tag="ACHBFA_000775"
     CDS             5177..5614
                     /gene="zapA"
                     /locus_tag="ACHBFA_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9394290.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5672..6220
                     /locus_tag="ACHBFA_000776"
     CDS             5672..6220
                     /locus_tag="ACHBFA_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM9394291.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6335..8695
                     /locus_tag="ACHBFA_000777"
     CDS             6335..8695
                     /locus_tag="ACHBFA_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9394292.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8782..9096
                     /gene="trxA"
                     /locus_tag="ACHBFA_000778"
     CDS             8782..9096
                     /gene="trxA"
                     /locus_tag="ACHBFA_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9394293.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9278..11056
                     /gene="uvrC"
                     /locus_tag="ACHBFA_000779"
     CDS             9278..11056
                     /gene="uvrC"
                     /locus_tag="ACHBFA_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9394294.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11201..12100
                     /locus_tag="ACHBFA_000780"
     CDS             11201..12100
                     /locus_tag="ACHBFA_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394295.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12093..13355
                     /locus_tag="ACHBFA_000781"
     CDS             12093..13355
                     /locus_tag="ACHBFA_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9394296.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13516..15414
                     /locus_tag="ACHBFA_000782"
     CDS             13516..15414
                     /locus_tag="ACHBFA_000782"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM9394297.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15744..16463)
                     /locus_tag="ACHBFA_000783"
     CDS             complement(15744..16463)
                     /locus_tag="ACHBFA_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9394298.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16653..16700
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16800..17132
                     /locus_tag="ACHBFA_000784"
     CDS             16800..17132
                     /locus_tag="ACHBFA_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394299.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17119..19122
                     /locus_tag="ACHBFA_000785"
     CDS             17119..19122
                     /locus_tag="ACHBFA_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM9394300.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19141..19611
                     /locus_tag="ACHBFA_000786"
     CDS             19141..19611
                     /locus_tag="ACHBFA_000786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM9394301.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19632..20216
                     /locus_tag="ACHBFA_000787"
     CDS             19632..20216
                     /locus_tag="ACHBFA_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM9394302.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20231..21217
                     /locus_tag="ACHBFA_000788"
     CDS             20231..21217
                     /locus_tag="ACHBFA_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9394303.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21207..21518
                     /locus_tag="ACHBFA_000789"
     CDS             21207..21518
                     /locus_tag="ACHBFA_000789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9394304.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21540..23321
                     /locus_tag="ACHBFA_000790"
     CDS             21540..23321
                     /locus_tag="ACHBFA_000790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9394305.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23314..24690
                     /locus_tag="ACHBFA_000791"
     CDS             23314..24690
                     /locus_tag="ACHBFA_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9394306.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24697..25332
                     /locus_tag="ACHBFA_000792"
     CDS             24697..25332
                     /locus_tag="ACHBFA_000792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9394307.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25362..26717
                     /locus_tag="ACHBFA_000793"
     CDS             25362..26717
                     /locus_tag="ACHBFA_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9394308.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         8853 a         4547 c         5868 g         7545 t
ORIGIN      
        1 caggaggagg agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta
       61 tgcgttagtt gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt
      121 agcttatggc ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat
      181 tgtgctttcg aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc
      241 agaagaagtc gaaggtggga aagtggcgta tcttgcccaa acacctggag tcgtgactag
      301 taaagacagt acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca
      361 attcatcatg cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg
      421 cgatattagt ttgaaagaag aatatggtct agccatagga gatacagtta agttatctgg
      481 tagtgacaag acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc
      541 agttctctat acaaccatca atgcttacca agaaatccgc ttcgaaaaag aagatacaag
      601 tgaaaatgca cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga
      661 tttagaacag atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt
      721 attgactttt ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat
      781 tttcatttat gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg
      841 gatcacagga ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt
      901 tggtattctt cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt
      961 tccattccaa agcaattggc tgttttttgg cgtcattagc ttgttgatgt tagtagtagc
     1021 tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat
     1081 tggataagaa ggaggaagaa catgaaagca atagaattta aagaagtaga aaaaagtttc
     1141 caagatggcg accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg
     1201 gaatttgttg caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca
     1261 ggcggattgc agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat
     1321 caaaaagaaa gcgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct
     1381 tccaatctgg ttccttttct gacagtaaaa aagcagttat tattagtaga taagattaaa
     1441 catgaacatc gtcaaaaaga agcaaacgaa ttatttgagc agcttggcgt ggataagttg
     1501 atcaacaagt atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt
     1561 gcattgtaca atgatccgtc cattattcta gcagatgaac caactgctag tttggattca
     1621 gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca
     1681 acgatcatgg ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg
     1741 gaagatggtg tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg
     1801 atacaacaaa gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa
     1861 gggatatctc ggcacttcaa caagagaaat cgcacaaaaa gttgggatca cccagcccaa
     1921 tctctatcat tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt
     1981 aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt
     2041 aaccttgaca aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat
     2101 gcttcacgat cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg
     2161 gaagaaaaat tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag
     2221 aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat
     2281 cactcggtcg aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata
     2341 tttgaatggt gtattaggag aagcgtaaag ctgttatata gggagaaaat agttaggtga
     2401 agaagcaaga tatgacgaag atcgttatat cttgcttctt tatataggta gtttttcata
     2461 aatacaaatt tgttattctc tcatgattat cagggtatat ttttatttat tatgtcggtg
     2521 ttccaggaag tgttttaatt taataacgaa gaaattaatt gtttgtagaa tgaaggaaac
     2581 tatttattgg gtagatgaat caccacgaat agtcaaaagg agatttcagg atatgccaaa
     2641 aatgaaaaaa atcattttag cagtaatgtt attttctacg gttatatctt ttacagcccc
     2701 ttttttgctt acttaagctg attttaagtt ttggccctta gtctggatct acgaggtgag
     2761 agtcaagaat gaaaaaaata attggagctt ttgtatttgc aatcattatc attattatct
     2821 gttttcaaat agatgttttt ggtcaaaaaa agatagttgg acaaacggtc agcagtacga
     2881 acagtacggt tacaaagaca aaggaatcat ttgatgatac agaacaagag acattaatca
     2941 aaatcggaaa aaatgaccgt acactatata cagatgactc gctaactacc ccacttgcaa
     3001 ctataaacgg cggagagctg gcggagtttc tttctgaaac aaaaaatgct tatcagatca
     3061 agacaaatga tggctatact ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga
     3121 atatacaaac aaaaccggaa gagctatctg atgcagtgat tgtattagat cctgggcatg
     3181 gcggaaatga tactggggca ttaagcaatg atgaacaaac agaagaaaaa gatataacat
     3241 taagtacagc cataaaagta aaaaaggcgc tagaggatgc tggagcaacg gtttatttga
     3301 cccatactac agatgaatta gtacagcttg gagatatatg cgactacagt gaagaaaaaa
     3361 aggcagatgt ctttatcagt ttacacgcag actctacgga atatgcgaat gaagcaactg
     3421 gaatcacgac atattattac tatggacaag aggaaacatt agcacagaca attgctgact
     3481 ccttcactga tctgcctctg gattcaagag gaatctccac tgggaactat caagttcttc
     3541 gagaaaatct tcaaccttct attttaatcg agatgggtta tatgaataat gatagtgatt
     3601 taaaggaatt agtaacagac agttatcagc agcagattgc tgcaagtttg acacaagcat
     3661 tgaccactta ttttcagtaa taaataagcc cagccaaaca aaaatagctc ctcatatttt
     3721 tcgtttggcc gggcttatta tcgcagatgc aagcttttaa acaccgttta aggaagaggt
     3781 cgtgagcctg ccgttcaact ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg
     3841 tttgatgact attgtgactt gtgaccgagg agttggcatg agaacaccgt ttaagaaaaa
     3901 ggttgtgaaa tcaacgtttt gttctgagta ttagagaaac aaacggaaaa atagttcctc
     3961 atattttgcc gtttgttgag ctaataccga ggatcagatg attgaacacc gtttattcgg
     4021 aacaagggac tgtgacatga cttttgtcac agttccatta tttttttagt agcttcaaag
     4081 ctttttctgt attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt
     4141 tctccaatag ttttgccgct acttggtcag atttcgttcc agctcctgac ggaagggtga
     4201 ttccagcttc agctgatgct ttgtctaact cttccaaaaa gctggcacga ctgatgatag
     4261 aagcagcagc aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt
     4321 gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat
     4381 cgatcaaaat cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt
     4441 gattgtgcaa agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt
     4501 tttctggaga taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct
     4561 ggatgatttg gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg
     4621 caagctgatc acgtttcaca taagcagcac atactgttag cggaccgaaa tagctgccat
     4681 ttccgacctc atcactacct aaaacggata attgactgat attttttggc aaagaagagg
     4741 ctgtttttgc tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg
     4801 ctgcattcgt accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt
     4861 ctttttttgc agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt
     4921 atgctttcat ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat
     4981 tctcctcgtt tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga
     5041 ttgcgagaca tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat
     5101 ttgtggagaa cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata
     5161 ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac aaagcggtga ttgccaacca
     5221 aacatatact attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat
     5281 caatgaacag ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc
     5341 aatcttgatg gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatattgga
     5401 gctggaagaa gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa
     5461 tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg
     5521 tcaagcagat cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg
     5581 aaaaggacaa attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat
     5641 ttaaaaaagt tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt
     5701 gctaattgcg tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat
     5761 tggatatgta ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt
     5821 ggaattatac attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca
     5881 ggtatttgcc tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt
     5941 gctaattggc tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt
     6001 gccgatcttg agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta
     6061 cgttactata tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa
     6121 tcaattcggc gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa
     6181 caagatgtat gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat
     6241 atttcgtttt ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca
     6301 gctctttttt catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt
     6361 ggaatttgaa aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga
     6421 agaactggca gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga
     6481 aactgaggat ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaaattga
     6541 aaatatccgt ccccatatga aacggattga gatcggagca gacttgaatg gagtggagct
     6601 tgcacaagtt tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct
     6661 ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat
     6721 ccctgttttg agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga
     6781 cgcttctcct gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg
     6841 tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat
     6901 caccatgcga aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg
     6961 cggtgtagtc cacgatcaaa gtgcttcagg tcagacttta tttattgaac caaaacaagt
     7021 ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg
     7081 tattttaagt gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatac
     7141 ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa
     7201 agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc
     7261 actgatcgat caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc
     7321 catcgtcatt acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct
     7381 gttacaactg atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg
     7441 tatttttgag gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc
     7501 gaccttttct tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag
     7561 tctggtatta tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc
     7621 aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta
     7681 tcctgaattg aaagtttatg gatataatcg ggcaaatacg attaatgcca gcatggagtt
     7741 tgatgtggat acattaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa
     7801 tgcgtttgaa atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca
     7861 gttgatgaat gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg
     7921 gaaaatggca gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact
     7981 tcacgatgat ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga
     8041 aaaagcaaag aaaaaagcaa atgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat
     8101 cattgctgat attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca
     8161 gttgatcgat gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa
     8221 taaagtattg aagaaggcga aagaacaaaa aacgttaaaa ccgggagacg aagtgttggt
     8281 tacgacttac ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga
     8341 aatcggtata ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa
     8401 agagccgaca caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa
     8461 tcaattggac cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt
     8521 ggattctgcc attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg
     8581 cgcacttcgc aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga
     8641 gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt
     8701 actaaaggta agcagatggt tcgctatcca agtatagaaa tgctatacta gtcatagatt
     8761 ttaaaaatgg aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa
     8821 acagataaag gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg
     8881 caagcaccga ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct
     8941 aaaatggatg tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca
     9001 acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa
     9061 gaacaactac gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga
     9121 gggtgggact gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa
     9181 attaatattt tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc
     9241 tataataact ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa
     9301 attagcttta cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat
     9361 catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggtag
     9421 ccataatacg aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac
     9481 agaatcgaat atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa
     9541 atacaacatt atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa
     9601 atatccccgt ttggtgatta ctcgtaaggt actaaaagac aaagcactgt attttggacc
     9661 atatcccgat gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct
     9721 gagaaaatgc aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg
     9781 cctgtgtcct tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt
     9841 gaaaaacttt ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga
     9901 aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc
     9961 cattgagaca atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt
    10021 atttggttat gcgattgata aaggatggat gtgtgtccaa gtgtttttcg taagacaagg
    10081 aaagctaatt gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt
    10141 cttgacttac ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt
    10201 gattcctgat acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca
    10261 accaaaacga ggagaaaaga aaaaattagt tgaattggcg agtaagaatg cctcagttgc
    10321 tttgaatgaa cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga
    10381 acgtttagga aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc
    10441 taatatcatg gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc
    10501 aagaaaagaa tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc
    10561 aatgagagaa gtcatctatc gcagatattc gcgagtgatt cgcgaagggt tgcctttacc
    10621 tgatttgatc ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga
    10681 aaatcaatta ggaatcgata ttcctattgc tggcatggta aaaaacgata aacataaaac
    10741 aaatgagttg ctattcggta cagatttggc tgtagtaccg cttgagcgaa attcaccaga
    10801 gtttttctta ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg
    10861 gcagctacgc agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc
    10921 aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac
    10981 gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa
    11041 attacatcag gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta
    11101 tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg
    11161 tctaaggata tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct
    11221 tgttaaaact tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg
    11281 aaaaatcatg gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat
    11341 cttggatttt ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga
    11401 gaaagattat gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac
    11461 gattcaagac caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga
    11521 accaaaaatc gacttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt
    11581 aaaatctttg tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga
    11641 acctaaattg atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt
    11701 gctaaaagat ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca
    11761 caatatggat aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat
    11821 ggttttgaat ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt
    11881 ggaatctggt ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct
    11941 tcatgaagac aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt
    12001 tactcgtgcg actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga
    12061 agagattttc aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag
    12121 atgtctattt aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta
    12181 tcatgctggg tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata
    12241 aaatcggcgt attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag
    12301 atgattttga gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag
    12361 aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt
    12421 atagcgaaaa ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct
    12481 tgcaatcgat gcttcgagcg agtcaattag gcatttcacc agaagaagta gctagcttga
    12541 gccaacaagc aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag
    12601 tcggagaaga taactcgaca gtccaatacg tcgtaagtta tgtagcaacg attgtcttgt
    12661 tcattattat tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca
    12721 cgcggatcat ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaat
    12781 taacaggtgt tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta
    12841 taggatacag tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat
    12901 tggataactt gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt
    12961 tgattttttc tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag
    13021 caaaagcaat tttgccagtg acttatctat ctctaggcgg atacatgctt ggattgatac
    13081 taggagcttc agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat
    13141 cttcatatat tatgccgatt cgcttagcaa atgagacagt cggtatgggt ggagcattga
    13201 tttccttgtt ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt
    13261 acaaatcgaa tgtactcgtt tatagcgaag gcggattatg gagttcattg aaacaatcga
    13321 tttcaattat gaggaatgaa cgaaaaaaag cataaaacaa tcctaaaata cagaaggaag
    13381 ctggacatct attgttccca gcttcttttt gtatagacct attttattta ttttactata
    13441 ttggatatat attcattgaa aatagtgtat aacaacttta taatattgag tataccaatt
    13501 tttggaggcg ttttaatgga ccgttattta gatttacttt cagaaaaatt cccctcaacg
    13561 gaggatgtca tcacggaaat catcaatctc gaagcaattt tgcagcttcc aaaaggaaca
    13621 gagttatttt tgagtgatat acatggcgaa tttcctgctt ttgatcatat tctgcgtatc
    13681 ggttcaggaa atcttaaaga aaagatcaaa gacctttttt ctgaacaaat gacggaaacg
    13741 gatatcagcc aattcactat ttttacggct tatccagaat atgcattaac gactgattgg
    13801 tacaaagaac aagataaatc gcagctgatc catcatttga tcgatctttt acggtttacg
    13861 acagtcaaat atacccggtc aaaagttcga aaaggattgc cgaaagaata tagctatatc
    13921 attgaggaac tcttgtatat cgatgaccgt atcaatggaa aaaaagacta tgtaaaaaaa
    13981 atcattgaac aacttgtttt gatgaaggaa gaagaaaaat ttttaacgaa acttgcacag
    14041 acgatccaaa aatctgtcat cgatcatttg catatcgttg gagatatttt tgaccgagga
    14101 actcaagcag ccaaagtgat ggatcgaatc atggattttt cttctgtaga tatccaatgg
    14161 ggaaatcatg atattttgtg gattggtgcg tattgcggct ctgaggcatg tttactgaca
    14221 cttttacgga ttgcagcacg ctataattat ttatttgaat tagagaaaga atatgggttg
    14281 aatttacgtc cattgttttc ttttgctcat caaacttatc agaagaatcc agtatttgcg
    14341 cctaaaaaca gtaaaaatct atccgaaaga gagcaagaag aattagaaaa aatccatcag
    14401 gcactttcta tccttcaatt caaatcagaa catcagttat tggatcgtcg acctgaattt
    14461 aaaatggacg atcgtaaatt gctggaaaaa atcgactatg aagcatcaac gattgattta
    14521 gaaggtcagc tctatgagtt gaaaggaact tgttttcaaa cgatccagcc agatgatcca
    14581 aaaaaattct tggaagagga acaaaaagtg attgatagtt tgatgtattc attccagcac
    14641 tctttgagaa tgcaaaaaca tatgacgttt ttgatcgaaa aagggggaat gtatctcacg
    14701 aacaatcagc atgtgctgtt tcatggctgt atacctgtag acgagaaggg acagctctta
    14761 gcatttgaat tagaccaatc ttatcgcgga aaagaactgc tgggcttttt tgaaaaacag
    14821 ataactgaaa gcgcaaaaga tctgactgca aaagaagatt tagcaaccga tttgatttgg
    14881 tatgcgtgga gtgggccatt ttcgccttta ttcggcaaga gtaaaatggc gacttttgaa
    14941 cgttattttc tcacagagcc tcatacgcac gtcgagaaaa gcaatcctta ttatcacttg
    15001 cgagatgaag agtggttcag tgaaaaaatt ttagcagaat tcgacacaaa agaagaaggg
    15061 tcagcaatca ttaatgggca cacgccagta aaagtaaaaa aaggggagtc accgatcaaa
    15121 gcaaatggaa aagtatttgt cattgacgga ggtttatcca aagcttatca aaaaacgaca
    15181 ggaattgccg gttattcact attatgcaat tcttatggtt tccaaatcgt gacacattat
    15241 ccgtttttgc ctattgaaca acaatttgca aaaaaatccg accaaacaaa tgtgaaaaaa
    15301 gtaatcgaac agcaactacc acgcaaactc aatcgtgata caacaaaagg tatggaactg
    15361 cagcagcaga ttacccagct caaaaggctg ttggactatc gaaaggacga ttgagaagaa
    15421 ttccagtgaa cattgttcca tagactggag actgagacaa aagtcgtgtc gcagtctctt
    15481 tttccggata aacggtgttc ttctgccaac tcctcggtca caagtcacac tattcaacaa
    15541 acatgaaaag ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac
    15601 gacctcttgg tataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc
    15661 aaaaatgtga aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt
    15721 tcacaacccc ctcagaaagt ctattaaaat tcattcacaa agaaatcgaa taagcgctgt
    15781 tcagatggat gtacacggtg ggtaagttcc ggatgccatt gaacaccgag gattttatgc
    15841 atatcatctt tgctttgaac cgcttcaacg agtccatcgt tagaaaaagc gatggctttt
    15901 aataaaggtg aaagatcttt gactgcctga tgatggtaag aattcacaaa atatggatcg
    15961 gggagaaggt tgcttaacaa actgtctgaa acaatcttga cttcgtgagt cgtaaattgc
    16021 ggggcagtgg gctgttgttc atgttttact gaccactttg gatataatga aagatcttga
    16081 taaagtgtac cttccaaaac gacattcaat agttggagtc cccggcagac tgcaaaaatt
    16141 ggttttcttt gtttcaatgc ttccaagatc agtgcctgtt caaattggtc tcgttgaata
    16201 tttgtttccg ttagctttgg atgaggatct tccatgtaaa gttgtggact gacatcttga
    16261 ccacctgcaa gaataagttt gtcgatttgt ccaatcagtt gaggagctga atctggagac
    16321 atgactggca gaataagagg caatccttga gcatctaaaa ctgcatccac gaatccttgc
    16381 ggtgtatagc tcacttgatt tccttggaaa gtatctgttg ctcgaatcag ttgatttccg
    16441 gcaatgccga tgattggttt catttgtatt ccccctaaag tagacttatt gtaacaagga
    16501 tttaaaaaaa attaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta
    16561 ctatctgtaa agagaaattt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat
    16621 ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt
    16681 tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta
    16741 gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag
    16801 tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgaaaagc
    16861 gtgctcaaac actgcggcag cagctgaaga tctatgcaac agaaaaagag caggaattgg
    16921 aaacgatcca aacaaactat cgacaacaac taaaacagtt ggtggaagaa aaagaacaat
    16981 ttgaaataca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc
    17041 aagcaacttt gaaagatcaa tatcaaaagc atcagagtca agcgatcggt gcgatcattg
    17101 aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat
    17161 aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaacgt tgaaatccgg
    17221 gatctgtatc aaaatagaac aaacaaccag tgggtggaga agtattttcc agataccacg
    17281 attattgata aagaagcaaa attaagcgct ttaggtaatc ggttgaaaga catccaagaa
    17341 gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt
    17401 caagaacttt ccttacaaga tttggaagcc aattattccg aagaaaattt cataaataaa
    17461 ttgaaagaag tccttgattt aaaagaaaag tgggaacaac taactgaaga acgtgatcgt
    17521 ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg
    17581 tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt
    17641 ttcaaggaag aactgagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca
    17701 aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgttat agaagaaatt
    17761 gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa
    17821 ctgcaagaga ccaagcagca attatccatt gttcacgagc agcaaaagaa gataacgaca
    17881 gcgttaggtc agtgcagtgg ctatatccaa gaatttgaat gggtagaaga agtcacactt
    17941 gccgttgcag aacgagaaaa aataaaagaa cggtttatcc atgcgactta tctgatcgtt
    18001 ttgcaaggtt gggtagatgc agaagaaaaa cagaatttgc tgcatgtgtt aaacgaaacc
    18061 gtatcagaag aagatatata cgtcagtttc gaaacaccaa actcagaaga gattcagcag
    18121 gaagtcccta ctaaattgaa aaaccatccg cttgtcgaac cttttgaatt gctgactgaa
    18181 atgtacagtc tgccaaaata tgaagaagta gatccaacac cttggatgac accattttat
    18241 cttgtcttct tcgggatgat ggtcgcagat gttggttatg gtttgctgat gctcattggg
    18301 acaatactag ctcaaaaact acttgttcta ccacgcggga tgaaacgttt cgtaaaattc
    18361 tttgagattc tctcgatacc atcgatcatc tggggattga tctacagttc atttttcgga
    18421 cagtcattac caaaagaact gtttggcatc cgcttgcctt tcccaatcct atctacgacg
    18481 gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca gattttggtt
    18541 ggattatttg tggcagcaaa ggaaaatatc aggagaaaag attacttgag tgctgtcagt
    18601 gatggatttg cctggcaagg gatcctaatt gggatcgtta ttgcgttggt tggtgccatg
    18661 ttgataaaaa atacagcatt tgtttacctt ggcgctgggc tatcgatcat tgctgcctta
    18721 tgtatcttga tcgttccgat tatccaaacg ccgtctaaag tcaaaggtgc tgccaaaggt
    18781 gcgtataatc tttacggact gaccggctat atcggtgatt tagtcagtta tacacgtttg
    18841 atggcattgg gtatttcagg cggaagtatt ggagcggcat tcaatatgct agttgctttc
    18901 atgccgccag ctgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg
    18961 aatatgtttt tgacactgct aagtgcctat gtccatggcg cacgattgca gtatgtcgaa
    19021 ttttttggga agttttatac aggaggcgga cgtgcctttg atccgctgaa aacggcggaa
    19081 aaatacgtca acatcaatca caagaaaaaa aacaataaat aattggagga tttttatata
    19141 atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcggtatt agcgatggca
    19201 acagcgacga ttttttcagg gatcggttca gctaaaggtg ttggaatgac aggtgaggca
    19261 gcagcagcat tgacaacaag tcaacccgaa aaattcgggc aggctctgat tctgcaattg
    19321 cttccaggca cgcaaggttt gtatggattc gttattgcct ttttgatttt tatcaactta
    19381 ggaaatgaca tgtcagttgt acaaggactg aactttctag gtgcttctct gccgattgct
    19441 tttacaggct tgttttcagg tattgcccaa ggaaaagtcg cagcggcagg tattcaaatt
    19501 ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca
    19561 tacgcgattt taggtttcgt tatttccttc ctattagtgt tgaatgccta agcataaagg
    19621 aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaacgaa gcagcagagc
    19681 aggaaagagc ggcgttagaa caagaagagc gaatgaagat cgatcaaaac tttgaacaaa
    19741 aacgtacgca agttgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattactcg
    19801 aaaaaaaata ccgtcagcta aggaatcgcc agcaagtaga agtacgacaa gaaaatttga
    19861 atgcgaagca ggaattttta agacgattgt ttgcagatgc ggtaactgaa atggaaaatt
    19921 gggatgaatc tgaacaaatc cagtttatca aaaatgcatt gtattcatta cctttgacag
    19981 gaaaagtagc attcattgct ggagaaaaat cagcagccta tctttctcag acgttgttgg
    20041 atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc
    20101 aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg
    20161 tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag
    20221 ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact
    20281 tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga
    20341 aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga
    20401 acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga
    20461 gcctgaaatc gtttggatct atacgatgcg ttatactttc cataatctga aggttttgac
    20521 aaaagctgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc
    20581 attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaacttc ctgaaagcat
    20641 tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat
    20701 tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg
    20761 atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat
    20821 ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc
    20881 gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac
    20941 gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa
    21001 tattgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga
    21061 agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt
    21121 agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg aatcagaaac
    21181 aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag
    21241 attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa
    21301 ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag
    21361 agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg
    21421 ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa
    21481 aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt
    21541 tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg
    21601 atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg
    21661 agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag
    21721 gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt
    21781 cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta
    21841 atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg
    21901 aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag
    21961 aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa
    22021 caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg
    22081 gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag
    22141 aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc
    22201 cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg
    22261 ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg
    22321 agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata
    22381 caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag
    22441 cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg
    22501 gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga
    22561 tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac
    22621 ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg
    22681 aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag
    22741 tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc
    22801 aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag
    22861 tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca
    22921 tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt
    22981 ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt
    23041 tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa
    23101 tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt
    23161 atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat
    23221 atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga
    23281 tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt
    23341 gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta
    23401 atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa
    23461 gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct
    23521 gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta
    23581 ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg
    23641 gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa
    23701 acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta
    23761 ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg
    23821 gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa
    23881 gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg
    23941 tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg
    24001 actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac
    24061 atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga
    24121 agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga
    24181 agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac
    24241 gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg
    24301 actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg
    24361 cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg
    24421 aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta
    24481 ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa
    24541 aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta
    24601 gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg
    24661 ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg
    24721 acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa
    24781 ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac
    24841 aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg
    24901 gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac
    24961 gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt
    25021 gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc
    25081 gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca
    25141 gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc
    25201 aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg
    25261 aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt
    25321 accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac
    25381 aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt
    25441 tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt
    25501 ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga
    25561 atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg
    25621 gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca
    25681 gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga
    25741 tgaagctcat gctttacatc ctaaaattgg cctttgtgat ccaattgatt ggtacggttg
    25801 ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt
    25861 tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca
    25921 atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt
    25981 taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc
    26041 attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct
    26101 ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa
    26161 acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc
    26221 atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg
    26281 ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt
    26341 cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag
    26401 tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga
    26461 ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg
    26521 tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa
    26581 tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg
    26641 tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa
    26701 gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa
    26761 aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg ttt
//
LOCUS       DAYYNG010000023        25987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000023 DAYYNG010000000
VERSION     DAYYNG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.023"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACHBFA_000794"
     CDS             237..1877
                     /locus_tag="ACHBFA_000794"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM9394309.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACHBFA_000795"
     CDS             2031..3353
                     /locus_tag="ACHBFA_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM9394310.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACHBFA_000796"
     CDS             complement(3415..3846)
                     /locus_tag="ACHBFA_000796"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9394311.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACHBFA_000797"
     CDS             complement(3910..4746)
                     /locus_tag="ACHBFA_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM9394312.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACHBFA_000798"
     CDS             complement(4936..5238)
                     /locus_tag="ACHBFA_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9394313.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACHBFA_000799"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACHBFA_000799"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9394314.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACHBFA_000800"
     CDS             complement(5692..5958)
                     /locus_tag="ACHBFA_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9394315.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACHBFA_000801"
     CDS             complement(6212..6937)
                     /locus_tag="ACHBFA_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9394316.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACHBFA_000802"
     tRNA            complement(7303..7373)
                     /locus_tag="ACHBFA_000802"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACHBFA_000803"
     CDS             7490..8242
                     /locus_tag="ACHBFA_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9394317.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACHBFA_000804"
     CDS             8258..8737
                     /locus_tag="ACHBFA_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9394318.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACHBFA_000805"
     CDS             complement(8988..10568)
                     /locus_tag="ACHBFA_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM9394319.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACHBFA_000806"
     CDS             complement(10561..11304)
                     /locus_tag="ACHBFA_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394320.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACHBFA_000807"
     CDS             complement(11480..12256)
                     /locus_tag="ACHBFA_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9394321.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACHBFA_000808"
     CDS             complement(12504..13340)
                     /locus_tag="ACHBFA_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9394322.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACHBFA_000809"
     CDS             complement(13351..14280)
                     /locus_tag="ACHBFA_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9394323.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACHBFA_000810"
     CDS             14472..14825
                     /locus_tag="ACHBFA_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9394324.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACHBFA_000811"
     CDS             complement(14899..15555)
                     /locus_tag="ACHBFA_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9394325.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACHBFA_000812"
     CDS             15781..17715
                     /locus_tag="ACHBFA_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9394326.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACHBFA_000813"
     CDS             complement(17801..19024)
                     /locus_tag="ACHBFA_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9394327.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACHBFA_000814"
     CDS             complement(19018..20889)
                     /locus_tag="ACHBFA_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9394328.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACHBFA_000815"
     CDS             complement(20910..22028)
                     /locus_tag="ACHBFA_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9394329.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACHBFA_000816"
     CDS             complement(22111..22944)
                     /locus_tag="ACHBFA_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394330.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACHBFA_000817"
     CDS             complement(22937..23854)
                     /locus_tag="ACHBFA_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394331.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACHBFA_000818"
     CDS             complement(24081..25205)
                     /locus_tag="ACHBFA_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9394332.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..>25987
                     /locus_tag="ACHBFA_000819"
     CDS             25388..>25987
                     /locus_tag="ACHBFA_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9394333.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDEL"
BASE COUNT         8048 a         5167 c         4380 g         8392 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaatta
//
LOCUS       DAYYNG010000024        24337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000024 DAYYNG010000000
VERSION     DAYYNG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.024"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..2477)
                     /locus_tag="ACHBFA_000820"
     CDS             complement(132..2477)
                     /locus_tag="ACHBFA_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM9394334.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2756..3658
                     /locus_tag="ACHBFA_000821"
     CDS             2756..3658
                     /locus_tag="ACHBFA_000821"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM9394335.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3869..5644
                     /locus_tag="ACHBFA_000822"
     CDS             3869..5644
                     /locus_tag="ACHBFA_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM9394336.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5710..7296)
                     /locus_tag="ACHBFA_000823"
     CDS             complement(5710..7296)
                     /locus_tag="ACHBFA_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM9394337.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7547..7771
                     /locus_tag="ACHBFA_000824"
     CDS             7547..7771
                     /locus_tag="ACHBFA_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9394338.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7913..9664
                     /locus_tag="ACHBFA_000825"
     CDS             7913..9664
                     /locus_tag="ACHBFA_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9394339.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9664..11448
                     /locus_tag="ACHBFA_000826"
     CDS             9664..11448
                     /locus_tag="ACHBFA_000826"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394340.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11783..12635
                     /gene="licT"
                     /locus_tag="ACHBFA_000827"
                     /pseudo
     CDS             11783..12635
                     /gene="licT"
                     /locus_tag="ACHBFA_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12732..14618
                     /locus_tag="ACHBFA_000828"
     CDS             12732..14618
                     /locus_tag="ACHBFA_000828"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9394341.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14659..15561)
                     /locus_tag="ACHBFA_000829"
     CDS             complement(14659..15561)
                     /locus_tag="ACHBFA_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9394342.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15791..17194
                     /locus_tag="ACHBFA_000830"
     CDS             15791..17194
                     /locus_tag="ACHBFA_000830"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9394343.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTTLQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17194..18627
                     /locus_tag="ACHBFA_000831"
     CDS             17194..18627
                     /locus_tag="ACHBFA_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9394344.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18772..19698
                     /gene="trxB"
                     /locus_tag="ACHBFA_000832"
     CDS             18772..19698
                     /gene="trxB"
                     /locus_tag="ACHBFA_000832"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9394345.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19911..21638
                     /locus_tag="ACHBFA_000833"
     CDS             19911..21638
                     /locus_tag="ACHBFA_000833"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9394346.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21654..22004
                     /locus_tag="ACHBFA_000834"
     CDS             21654..22004
                     /locus_tag="ACHBFA_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9394347.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22098..22958)
                     /locus_tag="ACHBFA_000835"
     CDS             complement(22098..22958)
                     /locus_tag="ACHBFA_000835"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM9394348.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23050..23478
                     /locus_tag="ACHBFA_000836"
     CDS             23050..23478
                     /locus_tag="ACHBFA_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9394349.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23640..23816
                     /gene="rpsU"
                     /locus_tag="ACHBFA_000837"
     CDS             23640..23816
                     /gene="rpsU"
                     /locus_tag="ACHBFA_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9394350.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23843..24289
                     /locus_tag="ACHBFA_000838"
     CDS             23843..24289
                     /locus_tag="ACHBFA_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM9394351.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7709 a         4353 c         5185 g         7090 t
ORIGIN      
        1 gtttttcaca tttttacata aatttgtctt aatatcgtag atgtaagctt ttgaacacca
       61 tttattcgga aaaagggagc tgtgacaaga tttttgtcgc agctcccttt aattctgatt
      121 aatctattag atcaatcatc tttatcgtct gattctttgg tctctggatt cgctctagca
      181 taagctgaag cggtgttcca gtaatctgta tagttttcat cagtaccgcc tacgccgaat
      241 ttaaaggcgc ttttcttcgg accatttttt gcccaaagtg actccactgt ttttcctggc
      301 gtttggatgg attttttatt gacagtaact tttccagctg tttgtcctgt gaacccagat
      361 acttttttct tcatcacatc ggatgataag tcaaatttct catcagtgcc gaaaatctca
      421 gggtttgctt ggtaagcttg attgattaga ttggcaagat aattagccga acgtattctt
      481 gcttggctgt ccattccagt attgtcatcg tgtcctgccc aactactgat agtgatagat
      541 ggagttgaga cgatcagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
      601 caatctgcat ttcctagatt tccgtttaag cttgagatga catctttgaa tggtgtagtg
      661 attttttcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttcggaa
      721 taaatacgga cagaattact ttcatgttta taaatcacat tacctgcatt atctgtgatg
      781 gaatcaatga tatagccttc ttcgtagact ccaccatttg ctaaggcctg gaatccattt
      841 gtctgttgaa gcgtcgttac atctactgtc cctaaaggag ccgattcaac gccccaatta
      901 tcatttgctg ggtagttcat cttcgccaag tagtgctcgt aagcgtattg tttcgatcca
      961 ccggaattca acacatcttg atataagtga taagctggga tattgttgga ccattccagt
     1021 gactcacgga cggtttggaa ggtgttcgac ccttcattgg tcgcattgac gattttctca
     1081 ccagcatttt ttccttcttg ccaagtcgtt gcataatctg atacacggga ttctgatccg
     1141 acaagtccca tatcgattgc aggaccgtaa acaagaaccg gtttgatgga tgaaccagct
     1201 tgacgttctg tatcaaaggc gtggttattt tgattctggc tgtaattccg accacccacg
     1261 aagccataga tacgtccggt tctgttatcc atcaatacat ttccagtctc cacttggcta
     1321 ccagcaccat catctaacat gtagccataa ttttctacgc cggtttgtaa tgcagcatag
     1381 atatctttat cgatcgtcga atggatcgta tatcctttgt tttggatcgt ttgctgtgct
     1441 ttttgataat aagcttcact gaccgatgaa tctgacatat cagcattatc tttttctgcc
     1501 aattgttgtg caataatcct tgttgcttcg ttcatcacgg tataatacaa gaattctctg
     1561 tcagtctgtt ccgcgatttg ttgtgggaga aagtcttttg taagatcata ggctttcgct
     1621 tcttcgtatt gttctttcgt aatttgtcct tcacggtaca tactgaataa aacaaaatct
     1681 tttctggcaa gaccggcaga aagatcttct tttagcgcgc ctgtattcgt gtatggacta
     1741 tacgtgatag ggctttgagg caaaccagcg atataagcag cttgtggcag agtcacatcc
     1801 tttgcatcta caccaaagat tcctctagca gcttcctgga ctccagcaat attttgcccc
     1861 ttattattcc gaccaaatgg tgagacattc aaatacgtag taatgatctc attttttgaa
     1921 aagtgttttt ctacttgtgc agaaagaacg atctcgtttg ctttacgttt gaatgttgtt
     1981 tcatcggtta ggatctgttg tttgactaat tgctgtgtca aagtggatcc tccagacgag
     2041 ccgatgccag tcgcttctga aactagtgct cgtaaaacag ctttagggac ataaccttga
     2101 tgtttatcga agtattcatc ttctgtactg ataatcgcgg ttttcaataa aggcgaaatc
     2161 ttatcagatg aaatagtcgt ccgcatcaga tctgtctgga tttttgatat ctctgtatta
     2221 tctgcataaa caatctttga cgtttccgta atgtttccta gttctgtctg cagttctttt
     2281 ttagtcggca attgtgtgtc ttcgacaaga taggcaaagt agccagcacc tattccgata
     2341 cctagagctg cgaacaacaa aagaagaaca gtaataaaaa cagtcaatga ttgaaacact
     2401 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
     2461 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
     2521 aataatagga caaaaaaaca gacaaggctg tttttgacag gaatttcttt ttcgaatgag
     2581 ataaaacgtg aggaaatgat cttttttatg aaagagttct taagcttttc tttagggaaa
     2641 gtttcagtta ctatggagaa cagggcaaag aaaaacaaac cattttcttt gtacatcatc
     2701 ttatcaatat acaggaattg ttcgaaacaa ttgcctttca aggaggaatc catccatgtt
     2761 attaggctca catgtcagca tgagcggaaa gaaaatgctg ttaggcgcgg cagaagaagc
     2821 agccagttac ggatcttcga cctttatgat ctatacaggc gctcctcaaa atactagaag
     2881 aaaagctatc gaagagatga atattgatgc cggaaaagca tttatggcag aaaacggact
     2941 aacaaatatc gtcgttcatg cgccttatat cattaatttg ggaaatacga tcaaaccaga
     3001 aatgtttcca tttgctgtac aatttctgag agaagagatt gtccgtgcgg aagctttagg
     3061 tgctactcaa atcacccttc acccaggcgc tcatgtagga gcaggggcag aagctgggat
     3121 tgctagaatt attgaggggt tgaatgaagt tttgacaaaa gaacagacgg cacagatcgc
     3181 tttggagacg atggcaggga aaggtacgga aatcggtcga acatttgagg aattagcgca
     3241 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgata catgtcatac
     3301 gaatgatgcc ggatataata taaaagaaga tttttcaggt gtaatggaag agtttgataa
     3361 ggtcatcgga ttagatcggc ttaaagtact gcatatcaat gattctaaaa atccacaagg
     3421 ttctcataaa gatcggcatg ccaatatcgg ttttggaaca attggatttg acacattgaa
     3481 caaaatcgtc catgatccaa tgtttgacaa catttcgaaa atattagaaa ccccatatgt
     3541 cggtaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatttcca tgttcaaagc
     3601 gcaagcattc gatcccgaag tgttccctga gttattagca gaagaagtca cactttaagt
     3661 tttctacctg acagcaacaa aaaaggattg ggcagtctgc cttgtccttt tttgtttaag
     3721 aaacaaagga gtgctttttg cattcgtttt cgccatgctc cttttgcccc ctctttttaa
     3781 attcagacaa acagggtata ctgatagaaa agtaaaaaac gaaaagtttc acaaacaagt
     3841 agcaaaacgt gattgaaagg acgaaaagat gctgaacatt ttaaacccct tgatcgacca
     3901 actaaaaaag aaacatacat actttattct tggcatagcc gtattattta caattattcg
     3961 gttgtattta tatagtaaag caatctatgg aacagatatg tcggcgacct atgatgatca
     4021 gctgctgatt cactatgctc aaaatatttt ggatggtaaa tggctagggg agtatacgac
     4081 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     4141 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     4201 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
     4261 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     4321 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
     4381 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     4441 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     4501 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     4561 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     4621 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     4681 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     4741 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     4801 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     4861 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     4921 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     4981 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     5041 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     5101 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     5161 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     5221 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     5281 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     5341 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     5401 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     5461 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
     5521 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
     5581 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
     5641 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     5701 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
     5761 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     5821 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
     5881 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     5941 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     6001 ggtcatcatc agtaaaggaa tcatcgaaaa tggcaacgca aaagccaaga acacctggga
     6061 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
     6121 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     6181 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
     6241 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
     6301 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
     6361 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     6421 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
     6481 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
     6541 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     6601 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     6661 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     6721 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     6781 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     6841 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     6901 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     6961 ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     7021 atactgtaaa agcatagcaa ttagactaga catcaaaata actgacatca ataaatattg
     7081 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     7141 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt ttccctttgg
     7201 tacatccact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     7261 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     7321 ctacgattac attctacacc tcagaaagaa aaaattaaag aaaaagattc ggtatgccta
     7381 attttttttc gtggtgtgtc ttgctggtta atgaaagaaa ttttgttctt ctctaaaaaa
     7441 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     7501 aaataaatca ttgagagcaa ttcggaaata gaaaggagag atttatatgg tatcaacagg
     7561 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     7621 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     7681 gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc gccagatgat
     7741 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt ttgcgggctg
     7801 attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta ttcagcaaaa
     7861 aagacaagca cagacaatcc tatgctcttg ataaagacaa aggaggagta aaatgtcgat
     7921 ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa ttgggatttt
     7981 ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg ggatcatcat
     8041 tgatgagatc gccgataaga atttatcaat aaagatcatt tcctggtgga tcttattatt
     8101 gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga atatttgggg
     8161 aagtgcagct cggttggaga gagaacttcg gcagcagcta ttcaaacatt ttacagaaat
     8221 ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg caacgaatga
     8281 tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag attcagtgat
     8341 tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct taacactgat
     8401 tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt caaaactcca
     8461 cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga cacaagaaag
     8521 tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc tcgcggattt
     8581 tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt tagactcctt
     8641 atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa ttattttagg
     8701 tggaaagtat gtcttggaag gcacgatctc aatcgggcag atggtctcat ttattagcta
     8761 tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg tattggaaag
     8821 aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc atatcgtgga
     8881 acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta tttcctcttt
     8941 taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga tccaagaagg
     9001 agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca tcaaattact
     9061 gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt cgatcaaaga
     9121 atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc acttcctgtt
     9181 ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag aagaagaagt
     9241 agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc caaaaggtta
     9301 tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac agaggatttc
     9361 gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg cactttctgc
     9421 agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa gaaaagagaa
     9481 aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag aaatcattgt
     9541 tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg cattaggtgg
     9601 ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag gaggcgaggc
     9661 ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa agaacagaag
     9721 aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt catcgttgct
     9781 atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat catccaaact
     9841 tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt gttttttgct
     9901 ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca atggtatttg
     9961 tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt tgaaaagctg
    10021 catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt ttctcgagtg
    10081 acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt attgactggg
    10141 atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga aattgccttg
    10201 tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca gaaattcagt
    10261 tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa attgaatgaa
    10321 tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt agctaaagaa
    10381 tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa aacgaattcg
    10441 attctgttaa gtccaatcat caattttctc tatgcattgg cgattgcgtt ggcgttgact
    10501 ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta tgcatttacg
    10561 acctatgtcc aggaattttt taatccaatg tctcaaatga tggacttttt gagcaccttt
    10621 acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga agaatatgca
    10681 ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga gtttcgcaat
    10741 gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt tgtcgctaat
    10801 ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc gatcatcaac
    10861 gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga tcatgacatt
    10921 cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca agatgccttc
    10981 ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat cacagacgaa
    11041 cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga attaccaaaa
    11101 ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca acgacagctg
    11161 atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga tgaagcaaca
    11221 gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa aatgcgacaa
    11281 ggacggacaa cgatagcgat tgcccatcgg ctgtccacga ttcgggatgc agaattgatt
    11341 ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact tctagcgcaa
    11401 aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa agaggttgtg
    11461 acagtagcat agtagcattt tgccctaagc gctagagtga aaataaaaca gagcttcgca
    11521 gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga cgacgacagc
    11581 tagtcaaaag cggctaataa ccactaagaa agcgttgaca aaagcgtttg ctttcgataa
    11641 tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt
    11701 ctcccatggt tccatggacg gctttttggg tttttctttt tgttttggat acagccctat
    11761 atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat
    11821 aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa
    11881 gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga
    11941 cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat
    12001 ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt
    12061 gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt
    12121 tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg
    12181 gaaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca gaagatgaag
    12241 ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccaa caagacaata
    12301 cctacctgtt cactataaca atacaggata ttctaaatat cgtccgctat tactataaga
    12361 ttacatttga tgaagattca gtccattttt ttcgattcgt cacacactta cgtttttttc
    12421 tgacacgcgt cctagatgga gaaaaatcag aagtgggaga agtttctgag gaattgtttc
    12481 aggttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata gcagactttt
    12541 tagccgagac gttcaactac cagatgtcca cggacgaaaa aatgtattta acgatccata
    12601 ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaaggagga taaattaaca
    12661 agaacgtatg aaataagggt agtgacattc agttggctaa accttccact aataaattgg
    12721 agggaataga aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg
    12781 ggaaggaaaa tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa
    12841 acgaagaaaa agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc
    12901 gagcgggagg gcagtatcaa gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg
    12961 ttataaaagc aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt
    13021 ttgatatgct tattgatgga atatcgggct gtttccaacc attccttgct atcctagctg
    13081 ctgacgggat gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg
    13141 gttctggcac atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg
    13201 ttattatcgg tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc
    13261 ttggcgcagc attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag
    13321 caccactaac gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat
    13381 tcggcattcc ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg
    13441 tgttgttagc tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga
    13501 atttcttcgt tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg
    13561 gaccagtatt cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac
    13621 tttcgccagt tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg
    13681 gtctccattg ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa
    13741 atattatgac acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct
    13801 actttaagct tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta
    13861 ttgtggggat tacagagcca gctatttatg ggtttacatt gccacgtaaa aaaatattta
    13921 tttttacctg tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt
    13981 cttggaatca aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg
    14041 atttaacaga tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatcctttg
    14101 cgctgacttt tttcttttgg aaggaggaag cagatgaaac agatgataga cagaaaagat
    14161 caggtaaaga aattgtaaaa acaccgatac aaggacaaat cgctccactg aatgcagcaa
    14221 aggatgcagc ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag
    14281 gagaagtacg tgcccctttt gacggaacag tcatgtccct gtttccgacg aaacatgcaa
    14341 tcgggatggt ttctgaagca ggtctggaat tgctgattca tgtcggattg gatactgttc
    14401 agttggaagg gaaatatttt gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg
    14461 atctgttgct tcgcttcgac aaaaagaaaa tcgaagagga aggatacagt ttagaaacac
    14521 ctgtgattgt catcaattac atggattatt tggatatttt ggaaaatcaa ggggaaacgg
    14581 tcgggccaga cgatgcactt atcaccgcac tgacctgaac ggttaggtga aagaatggac
    14641 taggcttaac tattcttttt agtccacttc tttattttca aaattgaaag gctctttcat
    14701 gatggctgag ctgatagggc gtttatcaaa tttttgtcct acagaaatta gatggactaa
    14761 attctgttga taattttctg caaaaacaca actatataac acgtctagaa gataacaaat
    14821 cgatgtattg atcgtaaaat taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg
    14881 caaagcggct gtactcattg aagatagcgt tgaatcaccg atactggtca aagctaaaat
    14941 tggcacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt
    15001 ataagaaatc agaatcccac acatatcact tgtgcaattg tacgcttcat agaacgattc
    15061 accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc
    15121 ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat
    15181 tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt
    15241 catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa
    15301 aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa
    15361 agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt
    15421 ggacgggtgg acataacatc gctcagcaat ctcttggatg gtcatttcgc caagtgctgg
    15481 agaatcgctt aagagaaact gaatcacttc attttctgaa ggagaaaagg agatttcttt
    15541 gattttttca cgtaataaca tgtagatacc ttcttcttgt ttttttacgt aaatcataac
    15601 ttttttgaaa atgatttgta aacggattta tgttatatag cttaataaag acattttttg
    15661 acaagatatt tacaaaaatc cagaagaaaa cactaacttt tgtaaatgtt actggaaaga
    15721 aacgaatttc tattgtttgt tttgtttaca aaaaagataa taaaagtgta aaaagtaaag
    15781 gagtgagaac atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac
    15841 ccccgagttg atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg
    15901 gctcgtagat gtagaagaag gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg
    15961 tatgtgggat gcttctccgt atgaagtcaa aatctatgca actctggatc gggaagaagc
    16021 attgaaagat gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat
    16081 caaagacgag cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg
    16141 cgggatattc aaagcttttc gtaccgttcc agtggttttg gcaatcgttg aagacatgaa
    16201 gcgtttatgt ccaaatgctt ggctgattaa tttcgcgaac ccaagcggga tggtgacaga
    16261 agcagttatc agatatggca aatgggataa agtgatcgga ttatgcaatg tgccagtaat
    16321 ggcgatgatg acagaaccaa caatgcttgg caagaaaaaa gaagagctga tctacaagtt
    16381 tgccggtcta aatcacttcc attggcacaa agtagcagat aataaaggaa atgaccgcac
    16441 ggcagaattg attgatcgtt tatacgatga agacaatgga ttgccgaaaa atatctttga
    16501 catcccgttt ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg
    16561 ctattactat cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg
    16621 tacaagagcg cagcaagtga aagaaacaga agcagagctg tttgaattat acaaagatcc
    16681 agaattggat cataaacctg aacaattgca aaaacgaggt ggtgcttatt attcagatgc
    16741 tgcctgtgaa acgattgctt cgatctatgg aaataaagaa acacagatcg tggtatctac
    16801 aaagaatgat ggagctgttc ctgatctgcc ggcagattgt gtagtagaag tcactgccta
    16861 tgttggcgca caaggcgcac gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg
    16921 ctggttgcaa gtaatgaaga atatggaatt gctgacgatc gaagcagccg tgactggtga
    16981 ttatggaaca actttgcaag cgtttacgat caatccgctt atacgatcag gaaagacagc
    17041 aaaacggatc atggatgagt tgtttattgc acatcagcag tatttaccgc aattcaagga
    17101 aacaatcagc cggttaaaag cagaaggaat cgaagtccaa gatgaactgg caagagaatt
    17161 atcaagcaat acacatgtaa tggaggaagt atgatgggat tcagaaaaga ttttttatgg
    17221 ggcggagcta cagcagccaa tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta
    17281 gcgaacgtag atgttgtgcc aatcggagaa gaccgttttc ctgtcattac tggaaaaaag
    17341 aaaatgtttg aatttgatga aaatcatttt tatccagcaa aaattgcgat cgatatgtat
    17401 catcattata aggaagatat tgctttattt gcagaaatgg ggttcaagac ttatcggatg
    17461 tccattgctt ggagtcgtat ttttccaatg ggcgatgaaa aagagccaaa tgaatctggg
    17521 ttgaaatttt atgaggatgt ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg
    17581 acgattacac attttgattg tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc
    17641 agagaactcg ttggttttta tgaaaatctg tgccgagtca tcttcaatcg ctataaagga
    17701 cttgtcaaat attggttgac attcaatgag atcaatatga tccttcatgc accattcatg
    17761 ggcgcagggc tgtattttga agaaggagaa aatgaagaac aaatcaaata tcaagcggca
    17821 catcatgagt tggttgcaag tgccatcgca acgaagatcg ctcatgaagt agatccggag
    17881 aacaaagtag gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa
    17941 gatgtttggg cttctagaaa gtcagatcga gaaaattatt tcttcattga tgtccagtca
    18001 cgaggagaat atcccgctta tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa
    18061 atggaacctg gggatgccgc attgctgaaa gaacatactg ttgattttat ctccttttct
    18121 tattattctt ctcgagtttc gactactgac aacgaactct tggaacagac tgcaggaaat
    18181 atctttgctt ctgtcaaaaa tccatatctg gaagcaagtg aatggggttg gcaaattgat
    18241 ccattaggct tgagaataac gatgaatgat atttatgatc gttatcaaaa accactattt
    18301 attgtagaaa acggcttagg tgcagtcgat acgcccgatg agaatggtta tgtggaagat
    18361 gattatcgaa tcgactatct ggcagcacat atccaggcaa tgaaagatgc agtagaatta
    18421 gatggagtcg atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt
    18481 acaggtgaga tgaagaaaag gtacggattc atttatgtcg atcgtgacaa cgaaggaaat
    18541 ggcacattag cccgttcgaa gaagaaatca ttcaactggt ataaaaaagt aattgattca
    18601 aatggagaaa atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc
    18661 ggatttttcg agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt
    18721 cgcattggct gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat
    18781 gtaatcgtta ttggcgcagg gcctgctggg atgactgctg ctttatatgc ttcacgttca
    18841 aacttatccg tattgatgat cgaacaaggc gctcctggcg gccaaatgaa caatacggca
    18901 gaaatcgaaa actatcctgg tttcgattca atcatgggtc ctgatctagc ttataagatg
    18961 tatgaaggtg tttcaagatt tgggacagaa aatgcttatg gaatcgtgca agatatcaag
    19021 gatcacggca gccacaaaga agttatttgt gaagataaaa cttacgaagg aaaaacagtc
    19081 attatcgcaa caggctgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct
    19141 ggacgtggcg tttcttactg tgcagtttgt gatggtgcat ttttccgtaa caaacgtcta
    19201 gtagtgatcg gaggaggaga ctcagcagta gaagaagcca tctatttgac acaatttgct
    19261 tctgaagtag tcatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac
    19321 cgtgcatttg cgaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt
    19381 ggaaatgaaa tggtagtgac aggtgtgaaa gcacgtaatg tcaaaactgg caatgagttt
    19441 gatttggcag cagacggagt attcatctat gtaggattgg atccattaac tgcaccattt
    19501 agagaagctg ggttgacgaa cgaagccggc tggattccaa cagacaacga aatgaagacg
    19561 aatataccag gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact
    19621 actgctgttg gtgatggtgg gattgctgga caacaagtgt ataaatactt ggaatcttta
    19681 gaagaaacaa ctgcataagt ttaaaaagag tgaaagaacg gtttcaattg ttcttttact
    19741 cttttttttg cgtgaaatct actgaatccg atttatgcga atacttgtaa ccgtgcttga
    19801 taaaagaatc ttttcttgat ttaagcgttt tttggtcctc atactttcat atctaataag
    19861 tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc atgtcttggg
    19921 aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta aaaaaacaat
    19981 taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca ccgttggaat
    20041 tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg aacatctata
    20101 cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt gaagaaacaa
    20161 aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa tttgccatgg
    20221 aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa agtttacgtc
    20281 caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga atcatggtaa
    20341 ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa gatgggggac
    20401 aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt
    20461 taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc acgatcatcg
    20521 gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt gatcatgaat
    20581 tggtggaaac aatgggtaaa gatttgaagc ttgtgtacac accacttcac ggaacaggaa
    20641 aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta ctagttccag
    20701 aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca gaagaacact
    20761 ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta ctgattgcaa
    20821 cggatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc tcctatgaag
    20881 tattgacagg aaaccaaatc ggagccctgt ttgctcaata tattttggaa gcacaccaat
    20941 cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct agcgaattgc
    21001 ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca ggattcaaat
    21061 tcattgctga aaaaattcaa caatttgaag aagatcattc ccatactttt atgttcggat
    21121 tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac gcgattcaag
    21181 cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa acattgtatg
    21241 acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg atttctgtga
    21301 caatgagcgg acaagaaggc tctgcaaaaa tctctgcttt gatggaaaaa ttccgtgaag
    21361 aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc aaagagttga
    21421 cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct gacgtattga
    21481 agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa
    21541 tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa aaagtagccg
    21601 cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga ctgatggaaa
    21661 aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca
    21721 cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt gaccccactc
    21781 gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag
    21841 cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga gaaaaccatg
    21901 tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat tctcacgtac
    21961 tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag gtgcaaatag
    22021 agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt tttttaagaa
    22081 aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata ataactatga
    22141 tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga taaagaagcg
    22201 acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc gagttcagct
    22261 cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga gcgcatccgt
    22321 tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat ttttttagga
    22381 tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag attggcaacc
    22441 tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct ggatacgcct
    22501 agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc gtattttaca
    22561 gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg aagggcgcca
    22621 ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg cggagtcatc
    22681 acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc gatcaattct
    22741 tgattgatca atgtgtgtag gacaagggct ttttctttta gtgcatcttg cagtgcctgc
    22801 atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc aacggtagga
    22861 tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga gtcagagatg
    22921 acaaaaaaag taatgatttc tgtagaatcg gaagacatgg ataaaacctc acttgcattt
    22981 tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt tgatttggag
    23041 gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt gaaaaataac
    23101 ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa gcgcaatcgc
    23161 tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg agtaagttac
    23221 gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga agaaaccgat
    23281 ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg ccatcaccat
    23341 cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc aatgaattat
    23401 ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat tttcggaaga
    23461 tgtgaaaatt gtcaataagg atttaaataa aataacattt gataatttta aaaaaaagca
    23521 agatttcctt gacggtcaaa acagcattag atataatatt tacaggacag tcttgttttt
    23581 tgagatatat ttcaataaca attacggtta ctttttaagc tcggagggag ggaattaaca
    23641 tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga tgctcttcgt cgcttcaaac
    23701 gttccgtgtc aaaagcgggt actttacaag aatctcgtaa acgtgaattc tacgaaaaac
    23761 caagtgtaag acgcaagaaa aaatctgaag cagctagaaa acgcaaaaaa ttctagttct
    23821 gtgatgaatt ggagttggta gtgtgtcact actaagtaga ttaaacgatg acatgaaaac
    23881 agcgatgaaa gcgaaagata aggaatcttt acaagtcatc cgaatgatca agtcatctat
    23941 acaaaacgag cagattaaag aaggtcacga cttgacagaa gaagaagaat taacggtgtt
    24001 gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag tttgaagaag caggacgtga
    24061 cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc gaaaaataca tgccggaaca
    24121 actttccgac gaagaaattc gacaacttgt tcaagaagca atcacacaaa ctggtgcttc
    24181 atcaatgaaa gaatttggca aagtcatggg tgcgatcatg cctaaagtca aaggaaaagc
    24241 agatggaaac caagtaaatg ccattgtgaa agaactatta caatcttaat tttatcattt
    24301 aaaggctctt cgtcaatgag tgttggtgaa gaaattc
//
LOCUS       DAYYNG010000025        24304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000025 DAYYNG010000000
VERSION     DAYYNG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.025"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..829)
                     /locus_tag="ACHBFA_000839"
     CDS             complement(<1..829)
                     /locus_tag="ACHBFA_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9394352.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="ACHBFA_000840"
     CDS             complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="ACHBFA_000840"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9394353.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3330..3509)
                     /locus_tag="ACHBFA_000841"
     CDS             complement(3330..3509)
                     /locus_tag="ACHBFA_000841"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM9394354.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3556..5379)
                     /locus_tag="ACHBFA_000842"
     CDS             complement(3556..5379)
                     /locus_tag="ACHBFA_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9394355.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5657..7936
                     /gene="helD"
                     /locus_tag="ACHBFA_000843"
     CDS             5657..7936
                     /gene="helD"
                     /locus_tag="ACHBFA_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM9394356.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8029..8676)
                     /locus_tag="ACHBFA_000844"
     CDS             complement(8029..8676)
                     /locus_tag="ACHBFA_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394357.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8855..9574)
                     /locus_tag="ACHBFA_000845"
     CDS             complement(8855..9574)
                     /locus_tag="ACHBFA_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9394358.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9705..10856)
                     /locus_tag="ACHBFA_000846"
     CDS             complement(9705..10856)
                     /locus_tag="ACHBFA_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9394359.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11073..11813)
                     /locus_tag="ACHBFA_000847"
     CDS             complement(11073..11813)
                     /locus_tag="ACHBFA_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9394360.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="ACHBFA_000848"
     CDS             complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="ACHBFA_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9394361.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="ACHBFA_000849"
     CDS             complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="ACHBFA_000849"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9394362.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12774..13424)
                     /locus_tag="ACHBFA_000850"
     CDS             complement(12774..13424)
                     /locus_tag="ACHBFA_000850"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9394363.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="ACHBFA_000851"
     CDS             complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="ACHBFA_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9394364.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="ACHBFA_000852"
     CDS             complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="ACHBFA_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9394365.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14867..15394)
                     /locus_tag="ACHBFA_000853"
     CDS             complement(14867..15394)
                     /locus_tag="ACHBFA_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9394366.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15574..16359)
                     /locus_tag="ACHBFA_000854"
     CDS             complement(15574..16359)
                     /locus_tag="ACHBFA_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9394367.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16352..17221)
                     /gene="accD"
                     /locus_tag="ACHBFA_000855"
     CDS             complement(16352..17221)
                     /gene="accD"
                     /locus_tag="ACHBFA_000855"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9394368.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17222..18601)
                     /locus_tag="ACHBFA_000856"
     CDS             complement(17222..18601)
                     /locus_tag="ACHBFA_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9394369.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="ACHBFA_000857"
     CDS             complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="ACHBFA_000857"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9394370.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19053..19529)
                     /gene="accB"
                     /locus_tag="ACHBFA_000858"
     CDS             complement(19053..19529)
                     /gene="accB"
                     /locus_tag="ACHBFA_000858"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9394371.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="ACHBFA_000859"
     CDS             complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="ACHBFA_000859"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9394372.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAIS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="ACHBFA_000860"
     CDS             complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="ACHBFA_000860"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9394373.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="ACHBFA_000861"
     CDS             complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="ACHBFA_000861"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9394374.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22559..22783)
                     /locus_tag="ACHBFA_000862"
     CDS             complement(22559..22783)
                     /locus_tag="ACHBFA_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9394375.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22836..23798)
                     /locus_tag="ACHBFA_000863"
     CDS             complement(22836..23798)
                     /locus_tag="ACHBFA_000863"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9394376.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23808..24248)
                     /locus_tag="ACHBFA_000864"
     CDS             complement(23808..24248)
                     /locus_tag="ACHBFA_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9394377.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6641 a         5418 c         4239 g         8006 t
ORIGIN      
        1 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
       61 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      121 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      181 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      241 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      301 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      361 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      421 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      481 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      541 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      601 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      661 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      721 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      781 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      841 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      901 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
      961 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1021 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1081 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1141 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1201 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1261 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1321 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1381 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1441 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1501 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1561 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1621 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1681 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1741 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1801 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1861 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1921 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     1981 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2041 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2101 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2161 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2221 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2281 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2341 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2401 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2461 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2521 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2581 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2641 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2701 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2761 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2821 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2881 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     2941 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3001 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3061 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3121 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3181 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3241 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3301 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3361 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3421 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3481 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3541 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3601 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3661 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3721 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3781 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3841 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3901 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     3961 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4021 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4081 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4141 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4201 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4261 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4321 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4381 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4441 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4501 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4561 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4621 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4681 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4741 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4801 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4861 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4921 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     4981 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5041 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5101 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5161 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5221 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5281 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5341 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5401 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5461 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5521 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5581 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5641 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5701 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5761 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5821 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5881 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     5941 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6001 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6061 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6121 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6181 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6241 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6301 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6361 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6421 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6481 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6541 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6601 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6661 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6721 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6781 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6841 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6901 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     6961 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7021 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7081 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7141 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7201 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7261 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7321 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7381 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7441 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7501 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7561 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7621 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7681 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7741 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7801 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7861 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7921 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     7981 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8041 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8101 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8161 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8221 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8281 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8341 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8401 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8461 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8521 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8581 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8641 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8701 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8761 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8821 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8881 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     8941 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9001 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9061 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9121 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9181 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9241 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9301 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9361 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9421 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9481 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9541 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9601 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9661 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9721 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9781 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9841 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9901 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     9961 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10021 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10081 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10141 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10201 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10261 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10321 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10381 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10441 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10501 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10561 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10621 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10681 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10741 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10801 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10861 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10921 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    10981 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11041 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11101 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11161 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11221 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11281 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11341 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11401 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11461 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11521 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11581 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11641 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11701 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11761 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11821 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11881 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    11941 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12001 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12061 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12121 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12181 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12241 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12301 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12361 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12421 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12481 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12541 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12601 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12661 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12721 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12781 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12841 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12901 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    12961 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13021 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13081 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13141 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13201 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13261 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13321 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13381 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13441 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13501 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13561 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13621 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13681 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13741 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13801 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13861 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13921 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    13981 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14041 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14101 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14161 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14221 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14281 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14341 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14401 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14461 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14521 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14581 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14641 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14701 gagacatctt ggatctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14761 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14821 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14881 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    14941 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15001 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15061 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15121 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15181 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15241 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15301 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15361 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15421 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15481 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15541 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15601 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15661 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15721 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15781 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15841 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15901 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    15961 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    16021 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16081 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16141 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16201 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16261 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16321 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16381 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16441 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16501 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16561 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16621 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16681 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16741 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16801 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16861 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16921 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    16981 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    17041 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17101 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17161 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17221 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17281 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17341 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17401 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17461 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17521 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17581 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17641 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17701 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17761 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17821 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17881 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    17941 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    18001 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18061 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18121 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18181 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18241 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18301 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18361 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18421 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18481 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18541 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18601 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18661 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18721 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18781 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18841 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18901 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    18961 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    19021 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19081 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19141 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19201 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19261 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19321 attatttctg gagcagttgt tgcttgttca gatatgacat tgactggctc cgaaactggc
    19381 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19441 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19501 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19561 ccgcattatg tccgccaaac cctaatgaat tacttattgc ataagtaaaa cttgcaggtt
    19621 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19681 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19741 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19801 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19861 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19921 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    19981 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    20041 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20101 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20161 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20221 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20281 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20341 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20401 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20461 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20521 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20581 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20641 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20701 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20761 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20821 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20881 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    20941 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    21001 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21061 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21121 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21181 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21241 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21301 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21361 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21421 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21481 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21541 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21601 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21661 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21721 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21781 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21841 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21901 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    21961 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    22021 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22081 tccagctggg gtagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22141 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22201 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22261 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22321 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22381 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22441 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22501 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22561 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22621 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22681 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22741 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22801 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22861 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22921 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    22981 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    23041 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23101 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23161 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23221 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23281 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23341 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23401 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23461 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23521 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23581 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23641 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23701 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23761 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23821 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23881 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    23941 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    24001 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24061 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24121 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24181 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24241 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24301 atat
//
LOCUS       DAYYNG010000026        24200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000026 DAYYNG010000000
VERSION     DAYYNG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.026"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            229..960
                     /locus_tag="ACHBFA_000865"
     CDS             229..960
                     /locus_tag="ACHBFA_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9394378.1"
                     /translation="MENSFKNKALYHQLVDLLKKRMESGMIPHDKLPSERELTAQYGV
                     SRTTVRLALQELENTGYIYRRHGKGTFVSDIKTAAADLAGAYSFTEQMKSLGRHPETR
                     ILSFEKMEADKFIAQRLNLPLGEAVFKLSRLRIADREPLMIEDSYLPVKFFLSLDDQL
                     LRSKPLYDLFSEDFKQTVRLADEELYASIASKDDAKQLMIPEGAPVLHLVRQTYNMKN
                     EIIEFTLSVARADQFHYQIRHIRNS"
     gene            973..2145
                     /locus_tag="ACHBFA_000866"
     CDS             973..2145
                     /locus_tag="ACHBFA_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9394379.1"
                     /translation="MFTIEKEKLEQMGAEITTREIRQQPELWKETIDLYQSAQGELEN
                     FLTEVQKQAKGQRTRVIFTGAGTSQYVGDTLVPYLRKHGDRQAFSFESIGTTDIVAEP
                     EEYLIKEEPTLLVSFARSGNSPESLAAVEIANQVVDTIHHLSITCAKEGKLAQISQAD
                     SKSFLFLMPVRSNDDGFAMTGSFSCMTLGALLTFDQTALAEKQNYVETLNALGNEVIA
                     REHDIEEIVALDFERIVYVGSGSLAGLTREAQLKILELTAGKIATVFDSSMGFRHGPK
                     SFINEKTIMFGFVNNDPYTRDYDLDILEEVHEDEIASGVFAITQPGKRNFSGDTFEFA
                     TGTSLLPDAYLALGYVMVAQTVALLTSVKVANTPDTPSPTGTVNRVVKGVTIHDYK"
     gene            2168..3175
                     /gene="lacD"
                     /locus_tag="ACHBFA_000867"
     CDS             2168..3175
                     /gene="lacD"
                     /locus_tag="ACHBFA_000867"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9394380.1"
                     /translation="MLTLTTGKKAAMDRLSTKDGIISALAIDQRGALKKMIKALNVEP
                     TDKHIEHFKELVSSELTPYASSILLDPEYGLPAAKVRHADAGLLLAYEKTGYDATTPG
                     RLPDLLADWSVLRLKEQGADAIKFLLYYDVDEDPAINHQKHVFVERLGSECAEEDLPF
                     YLELVSYDAQISDAGSFDYAKVKPHKVNDMMKEFSKPQYKVDVLKVEVPVNMNFVEGF
                     GTGEIAYTKEEAANYFLEQSQATDLPFIFLSAGVSTELFQQTLVFAKEAGSTFNGVLC
                     GRATWKNGVKPFIESGEAAARDWLQSEGRENIESLNNMLKQTASSWHDKVQVAEEEAV
                     N"
     gene            3187..4149
                     /locus_tag="ACHBFA_000868"
     CDS             3187..4149
                     /locus_tag="ACHBFA_000868"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM9394381.1"
                     /translation="MIVTVTMNPSIDISYPLEKLKLDTVNRTDQVSKTAGGKGLNVTR
                     VIHDLKGDVLATGVLGGFHGAYISEELKKAGIKQAFTEIQEESRDSIAILHEGNQTEI
                     LEAGPTVSEAEQQAFLENFQELVKEAEIVTISGSLAKGLPLNFYQRLVQLAEEQVVKV
                     LVDTSGESLRQVLTGEHKPFLIKPNLEELEALLGQPFSIDDLEELQSALAQPLFTGVE
                     WIVVSLGKDGALAKYRDSFYRVTIPTIKTVNPVGSGDATIAGFAYGLSQKMSVTELLK
                     LCMAAGMANAQEVRTGHVNLANVDMHFEEINVRKASRTRSAFEQ"
     gene            complement(4380..5090)
                     /locus_tag="ACHBFA_000869"
     CDS             complement(4380..5090)
                     /locus_tag="ACHBFA_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9394382.1"
                     /translation="MVIPKYQIIKDELKQQIISGKFENGDKFYTEAELAEMFSVSSIT
                     VIRALNDLVKDGFIVRHQGRGSFVSRARKGKIVEFSDIELFPISNDQVQVLSITRGND
                     PVYLSKLELPDTAFYYCIKRLRKAEETPYIYQQTYLPEQYVNPNYQELSYYNSIYQRF
                     KLDYNIHMNEEDFTETNEIKFPTPSDAAKLLEISADEPTVLQVRVTKRRDTQQMLEYV
                     ESYKKWNFYKIELVSKNN"
     gene            5261..7039
                     /locus_tag="ACHBFA_000870"
     CDS             5261..7039
                     /locus_tag="ACHBFA_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM9394383.1"
                     /translation="MKTFEIKEDFLLNGEAFKIMSGAIHYFRIDPADWYHSLYNLKAL
                     GFNTVETYIPWNLHEPKKGEFHFEGILDLERFLSIAKELELYAIVRPSPYICAEWEFG
                     GFPAWLLNEDTRIRTNEIGYLKHVADYYDVLIKKLVPHQLSNGGNILMIQIENEYGSY
                     GEEKDYLRSVRDLMINRGITVPFFTSDGPWRATLRAGSMIEEDILVTGNFGSKAEENF
                     ASMQSFFDEHGKKWPLMCMEFWDGWFNRWKEPIVQRDAEELAEAVKEVLIRGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLNEQGNPTEKYFAIQKMIHDTFPEIQ
                     QMEPLTKNTMEVKGIHLRNKVSLFSTLDTISQPVKTTYPETMEMLGQNTGYILYRTSI
                     KKDAEEERLRVIDGRDRSQLFLNQKLQATQYQTQIGEDIIVPMTEETNQLDILIENMG
                     RVNYGHKLFADTQNKGIRTGVMADLHFLTDWSQYCLPLDSCEAVDYSREWQPEQPSFY
                     RYEVVLDEVEDSFIDLSNFGKGVVFVNQVNIGRFWEVGPTLSLYIPKGLLKEGNNEIV
                     IFETEGTFQPEIQLVKKPLYKEMKEG"
     gene            7042..7521
                     /locus_tag="ACHBFA_000871"
     CDS             7042..7521
                     /locus_tag="ACHBFA_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9394384.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNITRIMVVDDEVAQNDIEK
                     SGLKLATPAGVKLSVLPIEKAANNILAGKYDSQRLLIIARKPDRLLKLVELGVPIEEI
                     NVGNMSQTDETRSITKSINVVDQDVEVFNQLHAKGVRLIAQMVPSDKAEDFMNLLAK"
     gene            7534..8430
                     /locus_tag="ACHBFA_000872"
     CDS             7534..8430
                     /locus_tag="ACHBFA_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9394385.1"
                     /translation="MDILWWQILLLTLYAGYQILDELQIYSSLSAPVFAGLFAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSVALGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDKLVEDMNYKGIERNFLMGAIPWSFSRMIPVFLAL
                     AFGGGLVEKVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHFPYLILGFT
                     LTALLGTVFTNMQVLGTGVAGVVKNFEGIFNSLPMLAIALIGFALAAISYKNGQMIPS
                     SPAPKEVASNDSDEGEIEDDEI"
     gene            8417..9226
                     /locus_tag="ACHBFA_000873"
     CDS             8417..9226
                     /locus_tag="ACHBFA_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9394386.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEIMKTHTQFFNTSNFFNTIVTGIDLAIEEKEGIGGKDTVSGMKA
                     GLMGPFAAIGDSIFAALIPTIFGALAANMAINGNPTGIFIWIIAQIAVMIFRWKQLEF
                     AYREGISLVTTMQHRLTALTDAATLLGVFMVGALVATMINVKFSWAPSIGDVTLNMQN
                     NLDMILPRLLPAGIVAAIYWMLGKKNMTSTKAIFIVLVVCVALSALGVISK"
     gene            9264..9668
                     /locus_tag="ACHBFA_000874"
     CDS             9264..9668
                     /locus_tag="ACHBFA_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9394387.1"
                     /translation="MGKQLVLVSHGLFCEELKKSTEMIMGPQESIHTVSLLPAESAED
                     FRAKFEAVIADLDDFVVLADLMGGTPCNVVSRMILEGQAIELYAGMNMPMVIDFINSE
                     LIGTELALVESATSNICHVNERIIGDDDDEDE"
     gene            9812..10405
                     /locus_tag="ACHBFA_000875"
     CDS             9812..10405
                     /locus_tag="ACHBFA_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9394388.1"
                     /translation="MDFNEKLKQLRQNKQWTQEELAERLFISRTAVSKWESGRGFPSI
                     DSLKAISQVFDISIDELLSNSELLSIAENEQQQQRKNFQHLLLGISDCMVGLLIVLPI
                     FGQAQGNHIAFVSLWQLSQETIIFKLIIFSFVILTMLFGIFELAFSRREQRIIGSVSL
                     ILSLFGVLLFIMTRHPYPATYVLMLLAVKGISLLNSR"
     gene            10514..11173
                     /locus_tag="ACHBFA_000876"
     CDS             10514..11173
                     /locus_tag="ACHBFA_000876"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9394389.1"
                     /translation="MKIAKKQLETAIGLLIVFILYTISLSLFDVQKIGPQHSEVGFST
                     INQYVFQKIGTSEFWYQVTEILGIFPLIIVGYFALRGFFQLYTRKKLLLVDKEILALG
                     FLYAGVAACYVLFEKVIINYRPILVDGQLEASYPSSHTFLAITVLLSAFYVLRKETGT
                     GKAIIAYVSLVVMIALVIGRFLSGAHWGTDIFGGILLGLTLASCYLAGIESSSKQKKE
                     R"
     gene            complement(11194..11266)
                     /locus_tag="ACHBFA_000877"
     tRNA            complement(11194..11266)
                     /locus_tag="ACHBFA_000877"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11231..11233),aa:Thr,seq:ggt)
     gene            complement(11384..12532)
                     /locus_tag="ACHBFA_000878"
     CDS             complement(11384..12532)
                     /locus_tag="ACHBFA_000878"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9394390.1"
                     /translation="MSKHKHQKRKKNKLTLVFPLFLILLAFSGMYAEHSYPEHHATKK
                     SSTPATKTTTDLTNQQPIEPKNATSAGIEQLLVEKNFSGTVLVFQHGQVILNKAYGWK
                     DQARRIHNTIHTQYCIGSVQKGQTAYLIMQAVKSGKISLEEKLSTFYPQISNSEKISI
                     KDMLNMSSGLRIDEKKIAALNTTDPQKILSETIRQTTVAANSSYSYQPVNYVLLAGIL
                     EQVYHTDYSTLFDKLLRKPMKLKETSFYSETNNQQALSYSESSSGPVYAPTTVSSYAD
                     ELGTGNVFMSGEDLYSYFQDLYQDKFLTTQERAALWQSYPGEKYASGIYNMTNNYHSR
                     GVKARFEALVDFSKDGQNGIILLSNVWTQPKGYNDIVSKLYKQVVAGS"
     gene            12663..12736
                     /locus_tag="ACHBFA_000879"
     tRNA            12663..12736
                     /locus_tag="ACHBFA_000879"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12697..12699,aa:Asn,seq:gtt)
     gene            complement(12771..13187)
                     /locus_tag="ACHBFA_000880"
     CDS             complement(12771..13187)
                     /locus_tag="ACHBFA_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394391.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            13579..14826
                     /locus_tag="ACHBFA_000881"
     CDS             13579..14826
                     /locus_tag="ACHBFA_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9394392.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            15096..17486
                     /locus_tag="ACHBFA_000882"
     CDS             15096..17486
                     /locus_tag="ACHBFA_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9394393.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNTLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            17535..19370
                     /locus_tag="ACHBFA_000883"
     CDS             17535..19370
                     /locus_tag="ACHBFA_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9394394.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            19445..20302
                     /locus_tag="ACHBFA_000884"
     CDS             19445..20302
                     /locus_tag="ACHBFA_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9394395.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            20292..21401
                     /locus_tag="ACHBFA_000885"
     CDS             20292..21401
                     /locus_tag="ACHBFA_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9394396.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            21740..22540
                     /locus_tag="ACHBFA_000886"
     CDS             21740..22540
                     /locus_tag="ACHBFA_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394397.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            22646..23434
                     /locus_tag="ACHBFA_000887"
     CDS             22646..23434
                     /locus_tag="ACHBFA_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394398.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(23451..>24200)
                     /locus_tag="ACHBFA_000888"
     CDS             complement(23451..>24200)
                     /locus_tag="ACHBFA_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9394399.1"
                     /translation="AHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         8070 a         3986 c         5151 g         6993 t
ORIGIN      
        1 ctctaattgc gtcaactggt attagatttt cataccaatt attgaaaagt ctatttgaat
       61 atagtatgaa agaaaaattt tactagcata ttttatctta cgaaaacttg ataaatcagc
      121 attttaagcc gatagtaaaa ttactaaaag atttattgtt gacaaaggta aaaaaaccac
      181 cttaccattg ggttgtggtt ataaccagtt taaaagaagg agaagcccat ggaaaattct
      241 tttaaaaaca aagctttgta tcatcaactt gtagatttat tgaagaagcg gatggaaagc
      301 ggcatgattc ctcatgataa gctgccatct gagcgggaat taacagctca gtacggagtt
      361 agtcggacga cggtccgttt agctttgcaa gagctggaaa acacgggata catttatcgg
      421 cgtcatggaa aagggacatt tgtctctgat atcaaaacag ctgctgcaga tttagctggt
      481 gcctacagct ttaccgaaca aatgaaaagc ctcggcagac atcctgagac gcgaatttta
      541 tcctttgaaa aaatggaagc tgataaattt atcgcacaac gactcaatct tcctttagga
      601 gaagcagttt tcaaattgag tcggctgcgg atcgcggatc gagagccttt aatgattgaa
      661 gatagctatc tgcctgtcaa attcttcttg tcattagatg atcagctatt aagaagcaag
      721 cccttgtacg atcttttttc agaagatttt aagcaaacgg tccgtttagc agatgaagag
      781 ctttatgcca gcatcgcttc aaaggatgac gcgaaacagc tgatgatccc tgaaggagca
      841 ccagtcctgc atttagtccg tcagacctac aatatgaaaa atgaaatcat tgaatttact
      901 ttgagcgtcg ctcgcgcgga tcaatttcat tatcagattc gtcatattcg aaatagttag
      961 gagaattaaa gcatgtttac aatagaaaaa gaaaaattgg aacaaatggg agcggagatc
     1021 acgacgcgtg agattcgcca acagccggaa ctttggaaag aaacgattga cctttatcag
     1081 tcagcccaag gggaattaga aaattttctt acagaagtcc aaaaacaagc aaagggtcag
     1141 cgtacacgcg taattttcac cggtgcggga acctcccaat atgtaggcga cacgctggtt
     1201 ccttatttgc gcaaacacgg cgatcgtcaa gccttcagct ttgaaagtat cggaaccact
     1261 gacatcgtgg cggagcctga agaatattta atcaaagagg aaccgacctt actggtatcc
     1321 tttgccagaa gcggaaacag tcctgaaagc ttggcagcag ttgaaatcgc aaatcaagtc
     1381 gtggatacaa tccatcattt atcaatcacc tgtgcaaaag agggaaaatt ggcgcaaatc
     1441 agccaagcag actccaagag ctttttattc ttaatgcctg ttcgttcgaa tgatgacggt
     1501 tttgccatga ctggcagctt ctcctgtatg acactaggcg cgttgctgac atttgatcaa
     1561 acagcactag cggaaaagca aaactatgta gaaacattga acgctttagg aaacgaagtg
     1621 atcgcccgtg agcatgacat cgaagaaatt gtcgcacttg attttgaacg aattgtttat
     1681 gtcggttccg gcagtttagc aggcttgacc agagaagcac aattaaaaat cctagaactg
     1741 accgctggaa aaatcgcgac agtctttgat tcatcgatgg gctttagaca cggaccaaaa
     1801 tcatttatca atgaaaaaac tattatgttc ggttttgtca ataatgatcc atacacgcgg
     1861 gattacgatt tagatatctt agaagaagtt cacgaagatg aaatcgcttc cggtgtcttt
     1921 gctatcactc aacccggaaa acggaatttc tctggggata cgttcgagtt tgcaactggg
     1981 acttctttac ttccagatgc ctatttagca ctaggctatg tcatggtagc acaaactgtc
     2041 gcgcttttaa catcggtgaa agttgcgaat acacctgaca cgccttcacc aacgggaaca
     2101 gtcaatcgag tggtaaaagg cgtaacgatt catgactata aataggaatt aactgggagg
     2161 aagaaacatg cttacattaa caacaggtaa aaaagcggcg atggaccgtt tatcaacaaa
     2221 ggatggcatt atcagtgcct tagcaattga tcaacgtggg gcgttaaaaa aaatgatcaa
     2281 agcgttaaat gtcgaaccaa cggataagca tattgagcat tttaaagaat tggtatcgag
     2341 cgagctgaca ccatatgcgt cttcgatctt attggaccct gaatacggtc tgcctgcagc
     2401 aaaagtacgt catgcagatg caggtctatt gctggcatat gaaaaaacag gctacgatgc
     2461 aaccacacca ggacgtttac ctgatctatt agcggattgg tcggttttac gactaaaaga
     2521 acaaggtgcc gatgctatca agtttttact ttattatgat gtggatgaag atcctgcgat
     2581 caatcatcaa aagcatgtct ttgtcgaacg gttaggcagt gaatgtgcag aagaagattt
     2641 gccattttac ttagaattgg tttcttatga tgcgcaaatt tcagatgctg gttcatttga
     2701 ctatgccaaa gtgaaaccgc acaaggtcaa tgatatgatg aaggaattct caaaaccgca
     2761 atacaaagtg gatgttctaa aagtggaggt cccagtgaat atgaatttcg ttgaaggctt
     2821 tggtactggt gaaatcgctt atacaaaaga agaagcggca aactatttct tagaacaaag
     2881 ccaagcaacg gatttaccat ttattttctt aagcgcaggt gtttcaacgg agctattcca
     2941 acaaacattg gtatttgcta aagaagctgg ctccaccttc aatggtgtgc tctgtgggcg
     3001 tgccacctgg aaaaatggtg taaagccgtt tattgaatca ggtgaagctg ctgctcgtga
     3061 ctggctgcaa agcgaaggtc gtgaaaatat cgaaagcttg aacaacatgt taaaacaaac
     3121 ggcaagctca tggcatgaca aagttcaagt cgcagaagaa gaagcggtga actaggaggc
     3181 gagctcgtga tagtaacagt aacgatgaac ccgtccattg atatttctta tccactggag
     3241 aaactgaagc tagacacggt caatcgaacc gatcaggtga gtaaaacagc tggcggaaaa
     3301 ggacttaatg tcacgcgtgt cattcatgat ttgaagggcg atgttttagc aacgggtgtt
     3361 ttaggcggtt ttcatggcgc ttatatctcg gaagaattaa agaaagcagg tatcaagcag
     3421 gcatttacag aaatccaaga ggaaagtcga gattcaattg cgatcttgca tgaaggaaat
     3481 cagacggaaa tattagaagc tggtccgaca gtttcagaag cggaacagca ggcttttttg
     3541 gaaaattttc aggagttggt taaagaagcc gagattgtca ccatttcagg cagtctggct
     3601 aaagggctcc cattgaattt ttatcaaagg ctcgttcaat tagcggaaga acaagtggta
     3661 aaagtccttg tggatacctc tggtgaaagc ctgaggcagg tgttaaccgg tgagcataaa
     3721 ccctttctga ttaagccaaa ccttgaagag ctagaagctt tattgggtca gccgttttca
     3781 attgatgact tagaagagct gcagtcagct ttagctcagc ctttatttac tggtgttgag
     3841 tggattgttg tgtcactagg caaagatggg gcgcttgcaa aatatcgtga ttctttctat
     3901 cgagtaacga ttccaacgat taaaacagtt aatccagtgg ggtcaggtga tgctactata
     3961 gctgggtttg cctatggctt atcacaaaaa atgagcgtaa cagaactgct gaagctttgt
     4021 atggcagccg gtatggccaa tgctcaagaa gtgcgaacag gtcatgtcaa tctagcgaat
     4081 gtggacatgc attttgaaga gatcaacgta agaaaggctt cgcgaactcg ctcagctttt
     4141 gagcaataag accaaaatat ttctaaattg ccaagaaaca cagtgatcga agagaactga
     4201 tgttgattgg tacctacttg gtgcttctca aatttcgaaa tatttttgct tattgccgaa
     4261 aaagctagtt cgtgaagccg gacatggata tgcctgctgt aaaacgattt attcatgtga
     4321 aaattttata cttttttcat agtaaaaaag atcgagcaat aagctcgatc tagtcaggat
     4381 taattgtttt ttgagaccag ttcaattttg taaaaattcc attttttata ggattcaaca
     4441 tattccagca tttgctgtgt atcccttcgt ttggtcacac ggacttggag aacagttggt
     4501 tcatcagcag aaatctccag cagctttgct gcatctgaag gagtcgggaa cttgatttca
     4561 tttgtttcgg taaagtcttc ttcgttcata tggatattat aatctagctt aaagcgttga
     4621 tagatggaat tgtaatagct cagttcttga taattaggat tcacatattg ttccggcaaa
     4681 taggtctgtt gatagatata cggagtttct tcggctttgc gcaagcgttt gatgcagtaa
     4741 taaaatgctg tatcaggcaa ttcgagctta cttaaataaa cgggatcatt tcctcgtgtg
     4801 attgataaga cttgtacttg atcattactt ataggaaaca actcgatatc ggaaaattcg
     4861 actatttttc ctttacgggc gcgcgagacg aaagaaccgc ggccttgatg tcgaacaatg
     4921 aatccatcct tgactaaatc attcaacgcg cgaatgacgg tgattgagct gacactgaac
     4981 atttctgcca actcggcttc cgtataaaat ttgtcgccat tttcgaattt gccagaaatg
     5041 atttgctgtt ttagttcatc ttttatgatt tggtattttg ggatgaccat aggaacctcc
     5101 aaacaatgaa taggaattaa aagtttcatt agaatttttt gattttgtgg ttatggcttg
     5161 attttaacac attccactaa aataaggaga gttgactttc agtatcgaat aatttaatat
     5221 attaatatat taaaaacgct ttcatttaag gagggagctt ttgaaaacat ttgagattaa
     5281 agaagatttt cttttaaacg gcgaggcctt taagatcatg tcaggagcaa tacattattt
     5341 tcggattgac ccagcggatt ggtatcactc actgtataac ctgaaagctt taggcttcaa
     5401 taccgttgaa acgtatatac catggaattt gcatgaacca aaaaaaggtg agttccattt
     5461 tgaaggaata ctagatttgg aacgcttttt atcaattgca aaggaactcg agttatatgc
     5521 gatcgtaaga ccatcaccat acatttgtgc agaatgggag tttggtggat tccccgcttg
     5581 gctactaaac gaagacacac gcatccgaac aaatgaaatc ggttatttaa aacatgtggc
     5641 tgattattat gatgtcctca taaagaaact ggttcctcat cagttgtcaa atggcggaaa
     5701 tattttgatg atccaaatcg agaatgaata tggttcatat ggagaagaaa aagactattt
     5761 acgttcggtt cgagatttga tgataaacag aggaatcacg gttccgtttt tcacttcaga
     5821 tgggccttgg cgtgcgacac ttagagcagg cagcatgata gaagaagata ttttagttac
     5881 gggcaatttt ggttcaaagg ccgaagagaa ttttgcatcc atgcagagtt tcttcgatga
     5941 acacggaaaa aagtggcccc tgatgtgcat ggaattttgg gatggctggt tcaatcgctg
     6001 gaaggaacct attgttcaac gagatgccga ggagttggcg gaagcagtta aggaagtctt
     6061 aattagaggc agtatcaatc tatatatgtt tcatggggga acgaattttg gttttatgaa
     6121 tggctgctcc gcacgaggag tcattgattt gccgcaaatt acttcttatg attacggggc
     6181 tccgctaaat gagcagggca atccaaccga aaaatatttc gcaattcaaa aaatgattca
     6241 tgatacgttt cctgagattc aacaaatgga accgttgaca aagaacacaa tggaagtaaa
     6301 aggaattcat ttaagaaata aagtcagctt gttttcaact ttagacacga tcagtcaacc
     6361 ggtgaaaacg acatatccag agacgatgga aatgctagga caaaatactg gctacattct
     6421 gtatcgcaca tcgattaaaa aggacgcgga agaagaaaga ctgagagtca ttgatggtcg
     6481 cgatcgtagt cagctatttc taaatcaaaa attacaagcg acacagtatc agacgcaaat
     6541 cggcgaggat atcattgttc cgatgactga agaaaccaat caattagaca ttttgattga
     6601 aaatatgggg cgggttaatt acggacacaa attatttgct gatacacaaa ataaggggat
     6661 tcgaacagga gttatggctg atctgcattt cctcactgat tggtcgcagt attgcttgcc
     6721 gctggattct tgtgaggcag ttgattattc aagagaatgg cagccagagc agccaagttt
     6781 ttatcgctat gaagtagtgc ttgatgaagt ggaagacagc tttatcgatc tgtcgaattt
     6841 tggaaaaggt gtcgtttttg tcaatcaagt aaatatcgga cgattttggg aagttggccc
     6901 aacactatcg ctttatattc ctaagggatt acttaaagaa ggcaacaatg aaatcgtgat
     6961 ttttgaaaca gaaggaacct ttcaaccaga gattcaattg gtaaaaaaac cattatataa
     7021 agaaatgaaa gagggataac aatgagtatt attggagtaa gaattgacgg acgtttaatt
     7081 cacggacagg tagcaaactt atggacaaca aaactgaata ttacgcggat catggtggta
     7141 gatgatgaag tggcacaaaa tgatattgaa aaaagcggac tgaagctggc tactccagca
     7201 ggcgtgaagc taagcgtgct gccgattgaa aaagccgcta acaatatctt agcagggaaa
     7261 tatgattctc aacgtctatt gatcattgcc cgcaaacccg atcgtttatt gaagctagtt
     7321 gagttaggtg ttccaattga agaaatcaat gttggaaata tgtcgcaaac agatgaaacg
     7381 cgttcaatta ctaaatcaat caatgttgtc gatcaggatg tagaagtatt caaccaatta
     7441 catgctaaag gcgttcgatt gatcgcacaa atggtaccaa gtgataaagc agaagacttc
     7501 atgaatctat tagcgaaata ggaggataag taaatggata tcttatggtg gcagatctta
     7561 ttattaacat tatatgcagg gtatcaaatt ttagatgaat tacaaattta ttcatcattg
     7621 agtgcaccag tctttgccgg attatttgcc ggcctagtaa tgggcgattt aaaagcagga
     7681 cttattattg gcggtagtat gcaattgacc gtactaggtg tcggtacatt tggaggcgca
     7741 tcaaaaattg acgctaactc cggaacgatt ctagcaacag ctttttcagt agcattggga
     7801 atgaatccag agcaagcgat cgctgcaatt gcggtaccag ttgccagctt gatgattcaa
     7861 ttagatattt tggctcgttt tgccaatacg tatttcgctc atcggattga taaattagtc
     7921 gaagatatga attataaagg cattgaacgc aacttcttga tgggggccat tccatggtca
     7981 ttttctcgca tgattccagt ctttttagcg ttagcatttg gcggaggctt agtcgaaaag
     8041 gttgttgctg tcttgaatgg tgatttgaaa tggttaggtg acggtctatc tgtagccgga
     8101 gcagttttac cagctgtcgg gtttgcgatt ttattacgtt acttgcctgt gaaaaaacac
     8161 tttccttact tgatcctagg gttcacgtta acagcgttac taggaacagt ctttacgaat
     8221 atgcaggttt taggcactgg tgttgcgggt gttgtgaaga atttcgaagg cattttcaat
     8281 tcacttccta tgttagccat cgcattgatc ggatttgctt tagctgcgat cagttataag
     8341 aatggacaga tgattccaag ttccccagca ccgaaagaag ttgcttccaa tgattcagat
     8401 gaaggagaga ttgaagatga cgaaatctaa ttataagttg acgaaggaag attttaagca
     8461 gatcaatcgc agaagcttgt ttaccttcca attaggctgg aactatgaac ggatgcaagg
     8521 atcaggctat ctttatacaa tcttgccgca attacgtaag atttatggcg atgacacgcc
     8581 ggagttaaaa gaaattatga agactcatac acaatttttt aatacgtcaa acttcttcaa
     8641 tacaatcgtg actgggattg acttggcgat tgaagaaaaa gaagggatcg gcggaaaaga
     8701 cactgtatca gggatgaagg ctgggttaat gggtcccttt gcggcaattg gggattcgat
     8761 cttcgccgca ttgattccaa cgatttttgg ggccttggca gcgaatatgg cgatcaatgg
     8821 aaatccaact ggtatcttca tttggatcat cgcacaaatc gcggtaatga tcttccgttg
     8881 gaaacaactg gaattcgctt atcgtgaagg aatctcttta gttacaacga tgcagcatcg
     8941 cttaactgct ttgacagacg cggcgacttt gcttggggtc ttcatggttg gggcgttagt
     9001 tgccaccatg atcaatgtaa aattctcttg ggcaccgagc attggtgatg tgacgttgaa
     9061 tatgcagaac aatcttgata tgatcttacc gcgcttatta ccagcaggaa tcgttgcagc
     9121 gatttattgg atgctaggta aaaagaacat gacttcaacc aaggcgatct ttatcgtatt
     9181 agtagtctgt gtggcattat cagcattagg tgtgatttct aaataagtta ggctgattcc
     9241 aatgaataat gaggagcgaa ccaatgggaa aacaattagt tttagtgagt catggactct
     9301 tttgtgagga attgaaaaag agtacggaaa tgattatggg accgcaagaa agcatccata
     9361 cagtcagttt acttcccgca gagtcagcgg aagattttcg cgcaaaattt gaagcagtta
     9421 ttgcggattt agatgatttt gtcgtgctgg ctgatttaat gggcgggaca ccttgtaatg
     9481 tagtctcgcg gatgatttta gaagggcaag cgatcgaact atacgcagga atgaatatgc
     9541 cgatggtgat tgatttcatc aatagtgaat tgatcggtac agagcttgca ttagtcgaat
     9601 ctgcgacaag taatatttgc cacgtgaatg aacggatcat cggtgacgat gatgacgagg
     9661 acgagtaaaa tctcatagaa gatgatatag aggcgtcgtt tatttgtgaa taggagctag
     9721 tttgaaagtc taaaagactt tcgaaccagc tcctttttct gttctgctat tatggtatga
     9781 ttgaggagaa aagaaagaat gggagattag agtggatttc aacgagaaac taaagcaatt
     9841 acgtcaaaat aagcaatgga cgcaggaaga attagcagag agactattta tctcacgaac
     9901 agcagtttcc aaatgggagt caggtcgcgg gtttcccagt attgattcct taaaagctat
     9961 ttcgcaggtt tttgatattt ctattgatga gttgctatcc aatagtgaat tgctgtcaat
    10021 tgcagaaaat gagcagcaac agcagcggaa aaactttcaa catttgcttt taggtatttc
    10081 agattgcatg gtcggcttat taattgttct gccgattttt ggtcaagcac aagggaacca
    10141 tattgcgttt gtctctttat ggcaactttc gcaggaaaca attatcttca agcttattat
    10201 tttttcattc gttattttaa caatgctgtt cggcattttt gaactcgctt tttctaggcg
    10261 agaacaacga attattggat cggtttcttt gattttatcg ttgttcggcg tactgctttt
    10321 catcatgact agacatccat acccggcaac gtatgtatta atgcttttgg cagtgaaggg
    10381 gatttcccta ttaaatagcc gttgatacgc atcgtcacat caaggtgacg gtgcgcttct
    10441 ttttgtttct ggcagtctct gatacgtttt atttggatag cttccaaata attgttagga
    10501 gaggattagt caaatgaaaa tagcaaagaa gcaattagaa acagccatag gattattgat
    10561 cgtctttatt ttatacacga tttctttatc cttgttcgat gtacagaaaa taggtccgca
    10621 gcattcggag gtgggctttt ctacaattaa tcaatacgta tttcagaaga taggtactag
    10681 tgaattttgg tatcaagtga cggaaatctt agggattttt ccattgataa tcgtggggta
    10741 ttttgcttta cgaggatttt tccaattgta tacccgcaag aagctcttac tagtggacaa
    10801 ggaaattctt gcgctaggat ttctttatgc tggagtcgcc gcttgctatg ttctttttga
    10861 aaaagtcatc atcaattatc gaccgatttt agtggatgga cagctagagg catcctatcc
    10921 gtcaagtcat acttttctgg caattacagt cttactgtca gctttttatg tattaagaaa
    10981 ggaaacagga acgggaaaag caatcatcgc ctatgtgagc ctggtggtca tgattgcgct
    11041 ggttatcgga cgttttttgt ctggagccca ttggggaacc gatatttttg gtggaatttt
    11101 attaggtctg acacttgcca gctgctacct tgcaggaatt gaaagtagtt ctaaacaaaa
    11161 aaaagaacgt tgaaaactca acgttcttag aagagccacc tgtcgggctt gaaccgacga
    11221 cctcttcctt accatggaaa cgctctacca gctgagctaa ggcggcatag ttatttaaca
    11281 caagatatag aatagcgaaa acaagcttat ttgtcaactg gttaaacaga aaaaaatgcg
    11341 gcattagctg ctgtgaattc tttatttata aacgagtcgt cgcttacgag cctgcgacga
    11401 cttgtttata tagtttagag acgatatcat tatatccctt tggttgagtc cagacatttg
    11461 agagcagaat aatgccattt tgaccatctt ttgaaaaatc aacaagggct tcaaaacgtg
    11521 ccttaactcc tcgtgaatgg tagttatttg tcatgttata gattccgctg gcgtattttt
    11581 cacctggata cgattgccat aatgcagcac gttcttgtgt tgttaaaaat ttgtcctgat
    11641 aaagatcttg aaaatagctg tacaaatcct cccccgacat aaacacattg cctgttccta
    11701 attcatctgc gtaagaagat acggtcgtag gagcgtaaac agggccggag gaactttctg
    11761 agtatgaaag agcctgctga ttgttcgttt ctgaatagaa gcttgtttct ttcagcttca
    11821 ttggttttct taaaagttta tcaaatagtg tgctgtagtc ggtatgatag acttgctcca
    11881 aaattccagc taacaaaaca taattgaccg gttgatagga gtaactagaa ttcgcagcca
    11941 cagtcgtttg acgaattgtc tctgaaagta ttttttgtgg atcagtagtg ttcaaggcgg
    12001 caattttctt ttcgtcgata cgtaacccag aactcatatt cagcatatct ttgatagaga
    12061 ttttctcact attagaaatt tgcggataaa aggtgctgag cttttcctct agggaaattt
    12121 taccagattt aaccgcctgc atgatcaaat aggcagtttg tcccttttga acggagccga
    12181 tgcaatattg tgtatgaatc gtattgtgga ttcttctagc ttgatctttc cagccataag
    12241 ctttattcaa aatcacctgt ccatgctgaa aaactaaaac agtaccgcta aaattttttt
    12301 ctaccaataa ctgctcaata ccagcggacg tagcattttt tggttcgatt ggttgctgat
    12361 tggtcaaatc agtagttgtt ttcgttgcag gtgtgctgct ttttttcgtt gcgtgatgct
    12421 caggatagct gtgttcagca tacattccgc tgaatgctag gagtattaaa aaaagcggaa
    12481 aaacaagtgt taatttattt ttctttcttt tttggtgctt gtgttttgac ataataaatc
    12541 ctctaattct atttgaaatc gttccatttt tatttcgata aaaattttca gcagttccat
    12601 ctaatttttg aaaattagga ttgcatcatg aaataaaaat tgatattata gtaaagtatt
    12661 cttccggcat agctcagttg gtagtagcgc atgactgtta atcatgatgt cgtaggttcg
    12721 agtcctactg ccggagtttt agagatgatc ttttgatcgt ctctattttt ttattttaca
    12781 aatgaataca gttcgtcgtt tttaccaaga atgattgttt gcccaacttt gagattgtca
    12841 ggtgatcctt cagcggtttg taaggagaac gttccgttga acttatcgga taccgttata
    12901 ggtagcttta attctgatcc ttcctcatct ttgagaattg ctgtttttcg ttcatcaaaa
    12961 taaacagtct ttattaaata atttttcgta gtgatctcag cagatttgtc tttttttgta
    13021 gtagtaacag tttctttaga attagaggta ttttctacga ctattccatc tttggcattt
    13081 gagcttgtgt ttatttgatt gaatattttc agcgacaaga taccgctaag aatagtcaat
    13141 atagaaacaa gtgtaattaa tactgataat ttcctttttt tgttcatttt ttcttaactt
    13201 cctttttgtt ttgattagtt agatattcct tatctaacaa gcctttttag gctattaaat
    13261 gaggataaac cccaaaaaaa tattgcaatt atgacagtgc aatggtacta ttattataca
    13321 ataaattttg cgaaaggggt aggtctaaat cgcaaacaaa aagaaattga acctagacaa
    13381 atacaacttg tacaccgcaa gggaagcaag tttgattttg ggtaaaggta gaaattatgt
    13441 tacaaacata atcaactatt atccaaatcg tttgcctaaa ggttcgttca agaaagtggg
    13501 tgacaaaacc tatattataa cggaagaagg aatagaagca ttgcgatcac aaacagaaaa
    13561 taaaaacaaa aagaaggaat gaatatgaag aaaaattcgc ttggtatggt tttatttgca
    13621 tcagctgtac ttgcggggtt atcagtacca gtaacctcac atgctgatga atggactgca
    13681 aacacaccgg atcaaatcca aattacaaca ggacaatcaa gttatgcgtt caaaaaagga
    13741 gatactattt ggtcaactgt agagaagctt aaaagcttag gttacaagac tactgttgct
    13801 gagtttgccg aattgaacaa tgttgatttg gcaaaaggag aacaacggac tatcccaatt
    13861 ggacgaactt tcaacatcag taaagataca ttaacagtta aggaagcaga tggcgaagta
    13921 gttagtcaaa ctgttcaaaa agataaacct gatcctgttg aaaatacgga gcaagagaac
    13981 aaaaaatccg agactgcaaa agaatcggaa ccaacagcaa acacttctga taagacagaa
    14041 acaagtgctt caagtgttga tggagcaagt tcatctaata caaataattc tacaagtgag
    14101 aaagaagcat cacagggaac aagtgaaagt agcactacgg aaactgatac tggaacatca
    14161 acaaataact cacaatcaac atcaaatgat gcaaatgaat caaccagtac atcttctagt
    14221 gatcaatcac aaacaggttc gagtgatgaa actgacagtt ctacttcacc aagtaaaccc
    14281 gaacaaccaa ctaatgttgt gctaggcgtt ccatttatta gtcaaggtaa cacaatgctt
    14341 tgcgaaggaa catctttgct agaagcgtta cattataaag gtgttgcaac aaatcaagat
    14401 ttaatgactt ttgttaaatc aatgccacta tctcctgata ataatccaaa taatggtttt
    14461 tctggtgaat ggagacacaa tgtggatgga acttatcaag gtatgaatcc ggctcctgta
    14521 gtttcatggg gtgttgctaa tggcggaaat gttgtagata tttctggttc atcggtacaa
    14581 ggattaaaag atgagattgt aaatggaaat cctgtagtag cgtgggtaac ttatgccttt
    14641 gaacctgctc aacataaatc aatgttttgg ggcgatgcta tttggaatcg acatgtagta
    14701 gccgttgatg gatttaaaga cggtttttac catgttgtcg atcctgtgtt tggggcaagt
    14761 tggattaatg caggatcatt tgaaacagca tataacgtat ctcaattagc ggtttcggta
    14821 cgttaataaa agagattgtt tatatactaa gtagttaagt atagtcttta tttgtccttg
    14881 cttaactact tagaaaactt gttgctcatg gtgggtagaa ctaaaagggg tgatgctatg
    14941 aatactagag cgttgaaaca gcttgctttc aacgaaaaaa gtgattaata ggtgtggtga
    15001 ttttaactta ggaatgggcg cgaaggtcat taccacaccg acatatcgtg gctcattttc
    15061 ttcgcctaac tggtataaag aaggagtaac gagagatgaa gcatgaaaaa acgataattt
    15121 taaaatcaaa aataatttat tgttttgcga ctttagtaat ggtgttatct acgcttataa
    15181 tgcctttttc agcaataaga gctgaagcag atgtcacaaa accgacaagt gcaaaattta
    15241 cagaaactgg cgtaaaaaca gacggttata ttagggttaa tatgccgaat catccaaatg
    15301 aatggatgat ttcaagtcaa tttaaagatt ctcatggaaa tattggatat tgtatggatt
    15361 cagaattgcc atccccaaca ggaagtggag ccggaagctt gaagtacaag ggagctggca
    15421 gtgatgagtt ctatcgtatg tttaaaggtg gttttccatc aaaaacagca aaggaactgg
    15481 gtgcaggaaa tgatacagaa gcatggtatg caacacaatt agtttcatgg gttttagccg
    15541 gaaactttaa agtttcacag attgtatggt cacatccaaa ccataccgcc gcagaaacag
    15601 ctcgtgtaaa aaaagcattt gaaaaaattt atgactatgc taagaatgga aaagatacgc
    15661 ccaacactga gttttctatc acagcttcta agacagctga cgaaggaaaa tatcacactt
    15721 tcacatataa aacagcatca aataagactg gtaatgcgaa gctcactttt acatcagcaa
    15781 aaccagctgg tatgaagatt tatgatgcag atggtaaaga aattactaac aacactgtga
    15841 aattaaattc aagttttact attaaagtac ctgtgactac accaagtggt actttgtcat
    15901 ttaaaggtac tgctaatgtt agcacaacca atccttttac ttttgatgga cgaggcgtgt
    15961 atcaagatgc cgtagttatg atcactactt cagaaacaaa ggactctaaa tctttgagtg
    16021 ctaaatggac aagagctgtt ggtcaggtaa cagttaagaa agttgatcaa gatggaaatg
    16081 ctttaaaagg agcggagttc actctaactg acaataacaa tgctaaatct gttaagacaa
    16141 ccggagatga tgggttagct acgtttgatt tgcaacaagg cttaacatac agcttagaag
    16201 aaacaaaagt tcctgaaggt tatcaaggtt catttaaaaa ggatggtatt actgttaata
    16261 ccgatggaca aaaatttagt tacactgcta cgaacacaat taaaaaaggt aaagtaaaag
    16321 ttaaaaaggt tgaccaagat ggaaaaccat tagctaatgc tgaatttacc ttgacggatg
    16381 attcatccca aacacaagca gaaaaaacag atgaaaacgg acttgcaagc ttcgatattg
    16441 tagcaaacaa gacctacagc cttgaagaga ccggagtacc aactggttat acaggcgcat
    16501 tcaagcaaga aggaattacc gttaaagatg atggacaaac ttttgaatat acagctacaa
    16561 acactttgaa aaaaggtgta gtaaaagttc ataaagtcga tcaagatgga aaacctttgg
    16621 caaatgcaga gttcacttta accgatgaaa aaggtgcaac acaagtagaa aaaacagatg
    16681 aaaatggact tgcaagcttc aatattgtag caaacaagac gtatagtcta gaagaaaccg
    16741 gagtaccagt aggttacaca ggtgcattta agcaagaggg tatcagtgtt aaagacgatg
    16801 ggcaaacttt tgaatataca gctacaaaca ctttgaaaaa aggtgtagta aaagttcata
    16861 aagtcgatca agatggaaaa cctttagcag atgcagaatt caccttaacg gatgaaaaag
    16921 gtgtaaaaga agttaaaaag acggatgcaa ctggtctggc tacgttcaac attattgcga
    16981 acaaaactta caacttagaa gaaacaaaag taccaacagg ttacacaggc gcattcaaac
    17041 aagaaggaat taccgtcaaa gatgatggac aagtctttga atatacagct aaaaacactt
    17101 tgaaaaaagg tgtgatcaaa gttcataaag tcgatgatca agggaaagca ttagcaaacg
    17161 cagaattcac tttgacggac gacaaaggtg taaaagttgt acaaaaaacg gacagaaatg
    17221 gcttggcaac atttaatatt gttgccaaca aaacgtacaa aatcgaagaa acacaaaacc
    17281 ctaatggtta tacagggaaa tggtctaaag aaggcatcac cgttgctgac gatggacaaa
    17341 cctttgaata tacagtaatt aatcaccttg tacctgaaac agtaagaaca ggcggaatcg
    17401 gtacatcagc cttccttctt gtatttctaa ttgcaggatt gagtatggtg ctactgtatt
    17461 atcgagaaca gcacaaagca aaataaaaaa gtgttaatgg aaaagaagta aatatgaaaa
    17521 aagaaggctg acgtatgaaa tcaaaaaaaa ttttttcaaa tttaatgcta tttggtactt
    17581 tgctaggagt gggattgggc gcaagctcaa tctcttctag ccttggacta ggggggcaag
    17641 tagcatatgc acaacaaaac tataattatc aaggaactgg aacgattcat atccataaag
    17701 taacaaatac taatggggat gggaaccctg tctcagatat tgataacaaa gggaaagaaa
    17761 tagctttacc aaatggtgta aaacctcttg aaggcgttga gttctcttat gtgaaactta
    17821 ctaaagatga agctaaatcc ttgggatgga cggatagtga tactacaaaa ggtcaacata
    17881 ttgatgatgc taaagcacga ttattactaa acaaaaaata cagtgaagta agtagaatga
    17941 agactgattc acaagggaat atagacatac ctatacaagt aaatacagac gatttaacag
    18001 ataacttgta tttgattgtt gaaacaggaa cagtcaaagg tgtagttgaa gaagcgtatc
    18061 ctatgattgt taacgtacct gaactaaacg gatcagatga tcaatacttt aattatgatg
    18121 tacatgtcta tcctaaaaac tatttccaat taaactcagc agagcttgat aaaaaagacg
    18181 aagatggaaa ggctcttgcc ggaactgttt ttgaattgcg aaaagatgat ggatctgatc
    18241 aggttgttgg aacttataaa acagacagca caggtaaatt aactgtagat ggtttggaat
    18301 taggtgacta ttatttccta gaaaccgatt tgggaacaga tgctaataaa acaacctatt
    18361 tactagatgg taaaaaattc aaatttagcg ttaaacgtgg ggagactgct aaagtagttc
    18421 ttgatgttac caactacaaa cgacctgtaa ttactaaggt aattgataaa gaaaccgttg
    18481 atactggcga agatgttcaa tatacattga aaccatcaat tcctggtaat attggtaatt
    18541 acgaaaaata tgttgtcaaa gatgttctag gtgcagagtt aacttatgat tcatcagaag
    18601 tgatcgaagg aatgacttta gatactgatt acagtatcga acgtgacgga caaacgacaa
    18661 actatgtctt tactgaatca ggtatggata agcttcagaa acaatttgat gcaggggatc
    18721 gcttctttac aattttgatg aacgctcata ctaatagtaa agctaaaatg gatacaccac
    18781 ttaagaatga tgtttcaacg atctacaaca atggatatga acccgaagac gaaacaccag
    18841 ttgatcacgc ttatacaatc attggtgatc accaattcat taaaacagat ggagatacaa
    18901 gcaaagcatt gagtggagca aaatttgtag tgaagaacaa aattgatccg aataaagtta
    18961 cttacctaaa acaagatcca acaacgaaat ctgttacttg gattgcagat aaatctaaag
    19021 ctactgaatt cgtttccgga tcagatggta agttccaaat tgaaggcttg caatatacct
    19081 atgatgtaga ttttgataaa caaggtaatc caacaatcac agataaaaaa atcaatgatt
    19141 atgctcttga agaagttgtt gctccttcag gatatacacc aactagaaat gatgttccat
    19201 ttgtggtatc gaatgatgat gcttctagcg aagtaacaga catcaagaac cttaaaacac
    19261 ctgaaacagt tcgtacaggt ggaattggaa cagtagtatt tctagtggtt gcaggtgtgg
    19321 caggaacagg gctatatgtt gtctacaaac gagaacaaca caaagcttaa tgaaataaaa
    19381 ttccctagag tgtttttggc tctagggttt ctttttaaaa agtgtgtgaa aagtaggtat
    19441 gagcatgaaa aagaagaata tcaagttgat catcttaaaa attgggatgt tgctatgttt
    19501 aggagtaggt ctatatcctg taattactca ggcttatacc tactaccaaa ctcaacaaac
    19561 actaaactcc tataaggaaa aatatgatga agcttctgaa aaggagaatg aagaagtctt
    19621 caaaagatat tcagaagtta ataggaaacg aaaagacaca gaacaaacct cagtcacgat
    19681 tcctgaagat aataacaata ttccgtcaaa tggaaatctt cagaaggata aattaggtga
    19741 attagtgggt gaaattctta taccaaagat taatgtggat ttgccaattt atgatggtgt
    19801 aaacgatttc caactatcag caggagcagg aatattaacc ggaactgatt atccaactgg
    19861 tgggattgga aaccattctg ttatatcagc tcattcaggc attcctaata aaaaatattt
    19921 tacagattta gagttgctca aacttggtga taaatttttt attgatattt cagggaaaaa
    19981 acttgcttat caagtagatc ggataaaaac aattctacca accgaatttg aggatttgct
    20041 tcctgtggca ggaaaagatt atgtaacact tatgacatgt acacctgttg gagataattc
    20101 tcataggtta ctcgttagag gggttagcat accttaccaa aaaagtattg ttgagaaaga
    20161 aaagaagaaa gaagatttaa aggtaaaggc gcagatgaac tggtatccta tcgtagttgt
    20221 aggggtttta ttaataatag ctgtgatagt cattattaaa aaaatagtag caaagagacg
    20281 aggaatgaaa aatgaagaat aaaatactac tttttttgac tcttatttgc actttggttt
    20341 taacaattta tcctgcaaat cagctctatc atcaattcaa aacaaaagag aatgtggcgg
    20401 aaatccaaga gaaaattgag aaaaaacaaa ctccttcaaa agaggaagtg gcaaaaaaag
    20461 tcgaacaaat aaaatcgaac agcggtaacc aaggattaga caatactcag aaaacagata
    20521 aaggagtaga caatactcag gaaacagacg gtggatcatt tgatattcgt gaagctgaag
    20581 gaaaggtcaa tccatacaca ggagatgttt tttcaaaaaa tcaaacgaaa gattttgaac
    20641 catctaccta ttcaggagcg gttggttatg tctatattcc aaaaataaat gtgctagagc
    20701 ttttgtttct aggcgcttca caggagcatt tatatagagg tgtggcacaa atgagtgcta
    20761 ctagtctacc tttatcaggt aaaggtcatc aaacagtaat agcaggtcat cgtggcggtt
    20821 atacgtcacc acaatttctt tatgttgatc aattagtagc cggagaccac atttatatag
    20881 attatcttgg tgaaaaagca gtttatacgg ttttccgaac agacgttatt agaaattacg
    20941 gtcagaattc aacactgact gatattcctg atttagatat gttaacactt tatacttgtc
    21001 atccatatcc tcagaactac gatcgtttat tagttagaag tctaagagat gaaaaagcaa
    21061 aatacatcac gccacaagta acagttggca ataccgcata cgctgatgcc attgatagcg
    21121 agtccgtttc agataagaca gaaactgtta catcttctaa tgatttattt gaagatgttg
    21181 atcgtgccaa caaggaatta gatggaaata atgatcttaa tattccggaa caaggtagcg
    21241 tagagatgca agacagaacg tttgctgaaa aggcaagttc aatatttaca ggaattacca
    21301 ataaacatca gaaaatgctt ttagtcattt ggactgtaat aggagcatta gcaagtctaa
    21361 aggttcttat tcttttaatc aaagagtttc gaaaagcata atgaagatta taaaaattga
    21421 tagagtacag taaaaaggga ttgatactat tattgatctc ttttttattt tgaatagagc
    21481 tagattcact tttaaataaa atcaaaactg atcttaattc taaagaaaaa gtcaagaaaa
    21541 aaatacaata aattttttta tcagaatatt tatcagaata ttattagaaa agataaactc
    21601 aaaagcttaa tttaataggg ttattggttg tatttagaaa taatgtatgg aaaagctata
    21661 aagcaataga taagatgtag aaaatcttat ttgtgaatga aaaacaagtc atcgtaaaat
    21721 attaatagga ggtgtagtaa tggtgggagt atcaaaaaca gctttagact cattagctaa
    21781 actatttagt agcaggtttg atggtgcaga atttgttgaa tatctaagcc aaaacgaaag
    21841 tggaaaaact ctgatcgaaa caatgaaaaa agactatttg aatgaaaatg actttccgac
    21901 atggacgaat attgaagatg ctacatggga tgtagtatgt acgatatatt ctccttgggt
    21961 aacgattgct gattttgttt tagataaaat tggaggggat acataccctg atgccaaatc
    22021 tcttgctaca agtgacttta aaacgttcta tggttatcta ataggtctaa taggcaatgg
    22081 aatagatttg aaagttacgc caaaacaaac acctgaagaa attgaaaaag aaaaaggaga
    22141 ggttcaaatg gcttatgcaa atgacagaga aaaagtaaaa ttattggaag gtaaattatc
    22201 tgaagaagaa cttggaaaaa tggtgcaaga aatcaatgaa gacagcaact attttcctga
    22261 attgaaggta tatcaaaatg acgaagattt ctttaatgaa atgtttagta atagtccatg
    22321 ggaagcagta agtaaaacaa atttaggaga atggaacttt caagatgatc tagttgcatt
    22381 tagcaatgat gggtatttac gatcatattc agaaagtgag tatagagctg aattaaaaac
    22441 gtatgaaacg gatattctga tgagctattt aaatctatta gagagtggtc agggagagtt
    22501 aagagataaa gttgaagaac tattaaaaga agagctatag ggattatttc aacataaaca
    22561 caagcagaaa actaccaaac aaaacgtacc atgcaaaaag tcggttaaac cggctttttg
    22621 ctctattatg agaggagatc aaaccatgag tgcaacaacc gatatggctt tagaagcagt
    22681 aataaaagat aatacaggaa atgtcgaaaa tgtagaagac tttagcgata ttgcaatcaa
    22741 tctttcagca tggatttcag ctgtaggaga agaaaatatc gaatttgaga tcaagaatat
    22801 ggatgactta aaagcaaaat ttccttttat tgcatcgaaa ctaagtcaag ttgccgagga
    22861 taaatcgtgg aatgaattag ttagtttttc cgaaaaaatt tatgatatta cgcaagaaga
    22921 tgagtgtgtt tcatgggaaa tgaataatca atacaagaaa attgacgctt tcgtcaaatc
    22981 acgtatagaa ggaacagctt ttgagtttga aggtatagat accgcagata attggttaaa
    23041 taaagaatcc tttattaaaa gtatagcgga tgatcttgaa aaacaatggt ctaaggaaga
    23101 ctatgcagag tacctaaaaa cagaaagaga tacagaagtg aaagaaaaac tcactgaggt
    23161 atgtttggat gaacatattg atcccaatat cgtaaaacaa gaagactatg aagtggttat
    23221 tgataaagtt cctactgatt ttgaggaagt tgtaaagtat atagttgaag aagcattatc
    23281 gcaatactct tcagatgagg aagtggacga agaaactgtt cgtaccagag tttggcaact
    23341 ggtttttgat gacaaagatt ataaatattt gagtgatcaa aactcggtta attggagtgt
    23401 acgattatta attgatcaaa atgatattga atagagctga aaagctctat tcagattgta
    23461 gacaaaaagg agttggtaat taagccactc ctttttgtta gtctttgcat aattttgaaa
    23521 taaaaagcca taggcttttt tgttttccat agccatgtgg ctagtttttt taaattcatg
    23581 gcagcaaacg taagcatcgt gtgcgtggcg actttttcaa gacctcgata tttcgtccaa
    23641 cgcataccat gcttttcttt tgcatctgca aatattcgtt caatggtttg ttttctcttt
    23701 ttgtaaattt ctctattcaa cgccgtatgg cgttggtgtt caagttcatc cataagatca
    23761 gcccaaatat gtcgagtcac tattttttga tgttttgtac tcaaagtaca attttcaagt
    23821 aatggacaac tcacacagaa ttttggattt gatttatagg ttcgataacc atcacgagtg
    23881 atagttgaaa ataacaacgg ttgattattg ggacataaat agcagtcaaa ataggcatca
    23941 taaacaaagt cttttttacg aaacattcct ttcactccat gaggtgcagt atatggaaga
    24001 atcgggtaga gtttcaagtg tgacagaaaa tgaacaaatt taggcgtctt atatcctgca
    24061 tcagcaacca cacgctttat atttggaaaa gctcttttcg actgtttaac cagatcgatt
    24121 gcgacttggc tgtcatgcac atttcctggt gtgattaatg tagataagat aaaaccgtta
    24181 tcttcacagg aagtatgtgc
//
LOCUS       DAYYNG010000027        24045 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000027 DAYYNG010000000
VERSION     DAYYNG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.027"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..663
                     /locus_tag="ACHBFA_000889"
     CDS             31..663
                     /locus_tag="ACHBFA_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394400.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(797..922)
                     /locus_tag="ACHBFA_000890"
     CDS             complement(797..922)
                     /locus_tag="ACHBFA_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9394401.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1056..1949)
                     /locus_tag="ACHBFA_000891"
     CDS             complement(1056..1949)
                     /locus_tag="ACHBFA_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM9394402.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2131..3057)
                     /locus_tag="ACHBFA_000892"
     CDS             complement(2131..3057)
                     /locus_tag="ACHBFA_000892"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9394403.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3142..3903)
                     /gene="pflA"
                     /locus_tag="ACHBFA_000893"
     CDS             complement(3142..3903)
                     /gene="pflA"
                     /locus_tag="ACHBFA_000893"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9394404.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4147..6378)
                     /gene="pflB"
                     /locus_tag="ACHBFA_000894"
     CDS             complement(4147..6378)
                     /gene="pflB"
                     /locus_tag="ACHBFA_000894"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9394405.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6655..9105)
                     /gene="parC"
                     /locus_tag="ACHBFA_000895"
     CDS             complement(6655..9105)
                     /gene="parC"
                     /locus_tag="ACHBFA_000895"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9394406.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9117..11162)
                     /gene="parE"
                     /locus_tag="ACHBFA_000896"
     CDS             complement(9117..11162)
                     /gene="parE"
                     /locus_tag="ACHBFA_000896"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9394407.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHVSGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11328..11762)
                     /locus_tag="ACHBFA_000897"
     CDS             complement(11328..11762)
                     /locus_tag="ACHBFA_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9394408.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11889..12527
                     /gene="plsY"
                     /locus_tag="ACHBFA_000898"
     CDS             11889..12527
                     /gene="plsY"
                     /locus_tag="ACHBFA_000898"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9394409.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12659..13534)
                     /locus_tag="ACHBFA_000899"
     CDS             complement(12659..13534)
                     /locus_tag="ACHBFA_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9394410.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13616..14407)
                     /gene="codY"
                     /locus_tag="ACHBFA_000900"
     CDS             complement(13616..14407)
                     /gene="codY"
                     /locus_tag="ACHBFA_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9394411.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14425..15825)
                     /gene="hslU"
                     /locus_tag="ACHBFA_000901"
     CDS             complement(14425..15825)
                     /gene="hslU"
                     /locus_tag="ACHBFA_000901"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM9394412.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15839..16387)
                     /gene="hslV"
                     /locus_tag="ACHBFA_000902"
     CDS             complement(15839..16387)
                     /gene="hslV"
                     /locus_tag="ACHBFA_000902"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM9394413.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16424..17329)
                     /gene="xerC"
                     /locus_tag="ACHBFA_000903"
     CDS             complement(16424..17329)
                     /gene="xerC"
                     /locus_tag="ACHBFA_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM9394414.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17527..18903)
                     /gene="trmFO"
                     /locus_tag="ACHBFA_000904"
     CDS             complement(17527..18903)
                     /gene="trmFO"
                     /locus_tag="ACHBFA_000904"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM9394415.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18872..20950)
                     /gene="topA"
                     /locus_tag="ACHBFA_000905"
     CDS             complement(18872..20950)
                     /gene="topA"
                     /locus_tag="ACHBFA_000905"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9394416.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21072..21929)
                     /gene="dprA"
                     /locus_tag="ACHBFA_000906"
     CDS             complement(21072..21929)
                     /gene="dprA"
                     /locus_tag="ACHBFA_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9394417.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21985..22752)
                     /locus_tag="ACHBFA_000907"
     CDS             complement(21985..22752)
                     /locus_tag="ACHBFA_000907"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9394418.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22745..23605)
                     /gene="ylqF"
                     /locus_tag="ACHBFA_000908"
     CDS             complement(22745..23605)
                     /gene="ylqF"
                     /locus_tag="ACHBFA_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9394419.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23865..>24045
                     /locus_tag="ACHBFA_000909"
     CDS             23865..>24045
                     /locus_tag="ACHBFA_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9394420.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFI"
BASE COUNT         6281 a         5159 c         4131 g         8474 t
ORIGIN      
        1 atatacttat caatttaatt tggagatatt atggaaattc aacgtagaac cactaatttt
       61 ttacatttag gctctttttt tgcaactttg acgatcttat tttttctatg ttattcatac
      121 cttaagacct cacatgcgcc ttctagtgca ttaggctggt tgcttttgga tcattctgat
      181 atcacagaag ccgaagaaaa tccgtttttt ttgattttgt cttctctttg cggcttactg
      241 ttttctgttt atttcggtgc ctattttttt catcgacatc ctggtacgtt aaacgaagct
      301 tcaagcgtat tgattgcatt taaaacgttt tcttttttag caattctttt ttattcgttt
      361 aatcacaata cgcttttctt ctttatcatg aatggtttag tcgtgatttt atccattttt
      421 acttatttta ttttttcact catttttagc caattgaaat gtccgctctt ctttcgatta
      481 gttcctgtca cctgttttat ttacagcttg ttttcctttt ttgtccttgc tttaatgcca
      541 ctgaccaacc cttcgacgat ccaagtcgca ggaaatctgt tggatatgct cttcatcgtc
      601 agtctaggtg tatttatgtg gcaaagaaaa aacaaacaaa ataaaaagca ttttgaagcc
      661 tgactattac gacttcataa atgctttttt agttttaaac acttcaatct aaaagaataa
      721 cccaagattg gataaattat taatgactcg ccattcgttt cttagtgatt ctggattatc
      781 aaatggactt cctaatctaa tcagctagtt cttcccataa aatgccatac ttgactgaat
      841 cataatattg attatttaaa aatctaactt ttcttatcac tacttccttt gtcattccac
      901 atttctcacc atttttttgc attcctttgt ttccagacca tgtagtgaat ctaataggtg
      961 gtagataggc aaaatactca aataattctt ttaaccataa aggaagagaa aggctcctcc
     1021 tgcaagagtc agtataattt ttgcttactg ctctcttagg ttttactcac taaaactgtt
     1081 ccatattctt tgatataaga aaatgcatgc ggtataaatt tcttgctcag ctcagaaaag
     1141 atttgtcgtt taagaaaggc catttcctcc attgtcaatg aaggatccaa tgttggtaat
     1201 tcaatctcaa aattgttttt ttcgacaata ggtaattcta atgtgaatga ttcttcttcc
     1261 tttatagtat tcgtcacaag tagagactga tggaaaatac taggccggcg atttttatat
     1321 ttattcttac gtatcctgtc aacgataact tctatttgat caaaaaagta cgattcattt
     1381 acgtctccaa taagttcggc taattctttg aatgccttcg ttacaggaag ctgattcttc
     1441 tgtacaaaaa agtaattagc caaagtaagc gggaagtgtt ctatagagga aagtgtgata
     1501 aaacggtact tctcatttcc tgcagttgtc ctcagcaatt ctgtctctcg gctgtttctt
     1561 actccataaa aatacgcttc tttccgttca tcttcgaaac aatacgccca aataacttct
     1621 cccatcgcta atttttgttc atcaacagag agtctcttca attgttcagc tatggtttca
     1681 gcagccgaag tggcttgttg caagtaatct ttaggatcaa atattggaaa gttcaaatga
     1741 tatcttcgtt cttccctctt gattaatcct gcatcgatca ataaatcaag aaaatgggaa
     1801 aatttatttt gcgaaaattc ttttttcaat tctcttagaa tcgtttcttg ttctcgatgt
     1861 tcatatagat aatcggtcaa tgaagaaaaa agcggatcat cggtcagttt ttgataaaag
     1921 ttttccttat tttttgaata atagttcata tatgttctct tttctatccg ttttatttta
     1981 cttaaaaggc ttttacgtaa aaaggaggaa gaacaagagt cataattgac tgttattctc
     2041 ctccatttct tcggccttta gtatgttcta tgtttctttt tatatcaatc gcttatccat
     2101 tcattttgaa tgcttcatac taccatttga ttagctaaat acagccgtca attgaggtac
     2161 gacttgtttt ttacgtgaaa cgacaccttt aagcaaagca cggttgttgg tcaaagttgt
     2221 ttggaaagct tcttctactt tttcttttcc tttaccagca acaagtaatt ctgaatcact
     2281 gtctaagatg tttgtgacaa ctaatacgaa cagatcgtag ccatttgctt tcatttcatc
     2341 ttccattgct gcttctagct ctgcttgtcg atccatcact tcattaaggt ccactgtatt
     2401 gacttgagcg atacgaacgt tggaactgcc cattgggaaa ctttttgcat ccaaatcaag
     2461 aagaaccgca gctgatttat cacttagatt tgttcctgct ttcaacattt caagtccata
     2521 agtgtttaga tcgacttcag cgattgctgc aagttctttt gcagctgctt catcttcagg
     2581 cgtacatgtt ggtgatttga acaacaatgt atctgaaatg atagctgaga gcatgattcc
     2641 tgcgatttct ttaggaattg ttacttggtt ttctttatat agttttaaaa taatcgtact
     2701 tgtacatcca actggttctg cacgatagta caatggatta gctgtttcaa agtttgcaat
     2761 acgatggtgg tctactacag ctaaaatatt tacttcgctg atatctgaaa tactttgttg
     2821 gaattcatta tgatccacaa gcatgacatc tgcattttct gctttagctg actctactac
     2881 tcgtggtgca gatagctgaa agtggtctaa tgcgtattgt gtttcttcat taggtgtacc
     2941 tagagcaact gcttctgttt ctttacctaa ttcattttgc aagtgtgaaa acgcaatggc
     3001 tgccccgatt gcatctgtat ctggattttg gtgaccaaaa actaaaattt ttgacatgtc
     3061 attcgctcct ttgtgatttc tcctatttaa taatagcaaa aaaacggaaa gaagcaatac
     3121 ttatcttctt tccgatgcga cctatcgcgt taaatatttt gtatactctt ctgtatgaag
     3181 caatcgttta gcattttcga cacgttcttt tgtaggtggt tcgatccctt ctaatggata
     3241 tttcaaacca agtgtttccc acttgtattt tcccatcgta tgataaggaa gaatctctac
     3301 tctgtctaca tttgacaagg tttgaataaa gttgtctagt cggatcaaat attcatcata
     3361 atcacttcgc tcaggaacta gtacatggcg aatccaaacc ggtttgttga tttttgacaa
     3421 atattgagcc atttctaaaa tattttcatt gtcccaatgc gttagttttt tatgctcttc
     3481 attatcgatg tgtttgatgt caaataacag taaatctgta tacttcatca attcttgaaa
     3541 acgtgaaaag aaaggttctt catacgtaaa aggttttccg catgtatcca atgtagtatg
     3601 catcttccgc tcttttgctt ttttaaatag ttcgatcaaa aaatcgatct gcaatagagg
     3661 ttctccacca ctaacagtaa taccaccttt atctccccag taaggtctga agcgttctgc
     3721 tttatctaaa agttcatctg ctgtgtattc tttccctccg ccaatattcc aagtatctgg
     3781 attgtgacaa aattggcaac gcatgcggca tccttgcata aacacgacaa agcgcagtcc
     3841 aggtccgtct actgaaccga aagtttcgat agaatgaaca tagccgattg ttttttcttc
     3901 catttaagtt cacatctttt ctttgatttc attttaagta caagttagct gtcgaagcga
     3961 agctttctga acaaaatttt tcgcaattgc cgcttcgtta tgtgtaaaat ttacagggac
     4021 ggaacataca agtcctgccc ctgtaaatcg tttctttcaa ataagcatac tgattattcg
     4081 attttgatgc gatggctatt caacgtcctt tcgacgctaa gtcgaaaaac ttttttcacg
     4141 aactttttac attctgtcgt gtgaagttct catcaagaca tctaattgtt gttctcttgt
     4201 caagtcacgg aatttaactg cataaccaga tacccggatc gtcaagttcg gatatttcat
     4261 tggattttcc attgcatcac gcaataaatc attactgaat acattgacat tcaaatgata
     4321 agctcctttt gagaagtatc catctaagac attacgcaag ttttcgattc ttgtatcttg
     4381 ttcttttcca agacctgaag gattgattgt ttgggtattt gaaattccat ccaatgcaca
     4441 gccgtaattc attttagctg ttgagttcag gctagctaat aacccacttt gttccgcatt
     4501 gtaacttgga tttgctcctg gcgcaagtgg ttttccagct tcacggccat caggagtatt
     4561 tcctgtattt ttaccgtaaa caacatttga agtaatcgtc aataatgaag ttgttggttt
     4621 tgagttgcgg taagttttat gacgtttgat ttgtcccatg aagtattcca agatccatga
     4681 aactaattca tctgcacggt catcatcatt tccgtattta gggaagtcgc catccacttc
     4741 aaaatcaact gctattcctt tttcattacg gattgctttt acttttgcat atttgatcgc
     4801 tgacaagctg tctgccgcat gagagatccc agcaatacct gtagcaaatg tgcgttgcaa
     4861 gtcagaatcc atcaaagcca gctgagctgc ttcataagaa tatttatcat gcatataatg
     4921 aatgatgttc aacgtattga catatagttc tgctaaccaa tccatcatat tcatgaagtt
     4981 atgtttcact tcttcaaagt ctagtatttc tgatgtgatt ggacgatatt ctggtccaac
     5041 ttgggcgcca gtcacttcat cgactccgcc attgatggca tataatagtg ttttagccaa
     5101 gttagcacga gcgccaaaga attgcatgtc ttttccgaca actgttcctg atacacagca
     5161 agcaatcgca caatcatcag aaccccattg ttcacgaagc tgattatcat tttcgaattg
     5221 aatcgaagaa ctttcgatag cgattctgct agcatatgtt ctgaatcctt ctggaaggtg
     5281 ttcagaatac aaaacagtca agtttggttc tggtgaaggt cccatatttg ttaatgtatg
     5341 aagcagacgg aaatcattct ttgtaactaa tgaacgacca tctaatccca tacctgcaat
     5401 ggataaagta gcccagatag ggaatcctga gaataattgg ttatattcag gtgtacgcgc
     5461 gaatttgacc atacgtaatt tcattactaa gtggtcgatc aattcttgtg cttctttttc
     5521 tgtgattact ccattatcca aatcacgttg gatatagata tcaaggaatg ctgagatacg
     5581 tccgattgac atcgcagcac cattttgtga tttgattgct gctaaatatc cgaagtatag
     5641 ccattggaca gcttcttttg catttgcagc tggtttagaa atatcatagc catatttcgc
     5701 agccatttct ttcatttgcg ctaatgcacg atattgttca gtgatttctt cacgtaaacg
     5761 aatcacttct tcactcattg tatgataacc agtcatatca tgatcttttt tcttttgttc
     5821 catcaaatag tcgattccat acaatgccac acgacgatag tcaccgatga tacgtccgcg
     5881 accgtaagcg tctggaagcc ctgtaatgat tttgttttta cgagcagcac gcatttcagc
     5941 agtataagcg tcaaatacac cttgattatg tgttttgcgc cagtcagtga aaatatgtgt
     6001 caattcatcg tttggtgtat agccattgct ttttagcgca ttgttagcca tattgattcc
     6061 gccgaacggc atgaatgctt gtttcaaagg tttgtctgtc tgtagaccga cgattttttc
     6121 caaatcttta tccaaatagc ctgcatcgtg tgaagtaata gttgatacga tttctgtgtc
     6181 catatcatag acaccgttac gctcgaattg gatatcattt aacttttgca attcatccca
     6241 taatttttct gttgcttctg ttgggccttc caaaaagctg tcatcaccgc gatattcagt
     6301 gtagttattt tgaataaagt ctctagtgtc tacgccttct ttccatttgg tacctttaaa
     6361 tccattccat tgctccattg tatggacctc ctaaatttct gttttatgta acagttttta
     6421 cgtgattagt ataacacagc accctatctt tgcaagggtt ttcttgtttt ttatttaaca
     6481 atttttatgt tttatttaca tatatttaaa tcaacgttga cataccaaca tttaagcatt
     6541 gtgaacactt tcatcttttt ttgcaaacgt ataaggattt attaaaaatg aatgaaaaag
     6601 agacgccacc tgtattaata cagatggcgt ctcttcatta aaatactaca acagttacga
     6661 ctcgatgaaa atctcaggag catcgataac ttcgatgact tctccacctt ttttctcatc
     6721 catcgcaaat gatccattcg atgttcgatc acctataggg aaatcagttg ggtcaatcgt
     6781 taactgctgc cctttttgat tcatcaaggt tatttctttt gatcttcctt ccgacatata
     6841 gataatacgg tgcggatttt ttttcagctc acgcatgacc gtcactcccc gcttcgcacg
     6901 tgaagtttgg ctgatttctt gagcaagcat gcgtttaaca gctcctcgct gagtaacgat
     6961 tacgatcggc gtatcacctt ctgctaacac aagcagcccg ttgataatcc tatctccatc
     7021 tttcaaattc atagacttca ctccagctgc ctttgctcca acaacgggaa catctgttaa
     7081 aggatagcgc aaacccattc cttgatgcgt tactaagaaa acatctaagc ctggctgatc
     7141 ttctttcaga tacacagcaa tcagttcatc ttcttccgcc tttagcttca tacagctgag
     7201 cggacggctc ttataggtgc gccatggttc gaactctgtc attcgtgttt gcttgatcaa
     7261 tccattcttg ctgatgaata caaatgtttt ctctgcttga attttttgat atgggaaaac
     7321 agctaaaatc gattcgtcta cagacagatt ggagattgtt tgagaaatgt gctcaccagc
     7381 atctttccat ttcaaatcgg gtagttcatg gacagggcgg tagatcacat ttgctttatt
     7441 tgtgatcaga agaacatggt ccaatgtact caattcacct gagtatagca agtagtcgcc
     7501 ttctcgcatc ccgatttctt ctggtttgga agcactataa gaacgaaggc ttgttcgctt
     7561 cacatagcct tctcgcgtca cactgacaat tacgtcttct tgtgccacta agacttgcgt
     7621 atcaattttg atttcttcga tttcgtcttc gattgtcgtt agacgagcgg aagaataatt
     7681 ttttttcact tctcgcaatt cttttttcat aacagagaaa agctctttgt catttgaaag
     7741 gattttattc aactctgtga tttgcgcaat caagctttcg gattcctttt gaagctctgt
     7801 gatatccgtg ttcgtcaaac ggtataattg caatgtgaca attgcttctg cttgttcttc
     7861 tgtaaattga aagacgtcga ctaaattctt tttggcgtct tttttgtctt tactttcacg
     7921 aatcgttgcg attacttcat ccagaatcga taaagctttc atcaatcctt ctacgatatg
     7981 ctgtcttttt tgagctttgg ctaaatcgaa ttgactacgt tttgttatga cttgctttcg
     8041 atgctcgata taacttctta aaatgtcttt cagtccaact tgatgcggag tcatatggtc
     8101 gatagcgacc atattgaaat tgtaattgat ctgtagctca gtgtttttga acaaataatt
     8161 caaaattcct tgagcattgg cgtctttttt caattcgacg acaatttgca gacctgttct
     8221 atcactttca tctcgtactt cagctattcc atcgattttc ttattcaaac ggatctcatc
     8281 catttttttc accaaggtcg ctttatttac ttcatatggg atttctgtga tcacgatttg
     8341 ctgtttatta cccttcattg cctctatctt tgttttcgaa cgaagaataa cttttcctcg
     8401 tcctgtttca taagcttttt tgatttcatc ttttccttgc aagatccctc ctgttggaaa
     8461 gtcaggtcca ggaatatatt ccatcaattt ttcaaggctc gcttgtggat gatcgatcat
     8521 atagattgtg ccatcgatca cttcagctaa gttatgagtc gggatttctg ttgcatatcc
     8581 agcagatata cctgtcgatc cattgacgag aaggtttggg tatcgagctg gaaggactgt
     8641 tggttctttt tctgtatcat caaaattcca cacgagatcg actgtttctt tctcgatatc
     8701 tgctagcatc tctccactta acttggataa tctggcttca gtataacgca tcgcagcagg
     8761 tggatcaccg tccatactcc cgttattccc gtgcatttcg atcagtactt cccgcaactt
     8821 ccagtcttgg ctcatgcgga ccatcgcttc ataaatactg atgtcaccat gaggatgata
     8881 attccccatg atatttccga cagattttgc tgatttacgg aatcctttgt catacgtatt
     8941 gccatctttg ttcatggcaa ataatatccg gcgttgcact ggcttcaatc catcacggat
     9001 atcaggcagt gcacgttctt ggataatata tttggaataa cgtccgaagc ggtcacccat
     9061 cacttcttct aaagttaatt cttgtacttc ttgtcgattt tccatcaggt gacctcctat
     9121 tctacttcaa ataactgatt gttttcattt ttatctgctc gttcttcatc taataaatca
     9181 ttagaaactg aaggagagat ttcagtatcc tcttttttat ctaagatact gccatcttct
     9241 tcgagcgtaa attgcacgtg attttcgatc cattttcttc gaggttctac tttgtcaccc
     9301 atcagtgtgg ttactcgacg ttcagcttgc gcagcatcat cgatacggac gcggatcaac
     9361 gtacgggaag tcggatccat cgttgtttcc cacaattgtt ccgcattcat ctctccaagt
     9421 cctttatatc gctgcagcat atatcctttg ccgacttttt taatcattgc tgccaattcg
     9481 tcatctgtcc acgcatattc aatgacttgc ttctttcctt gtcctttgga tactttatat
     9541 aaaggcggta acgcgatata tactttccca gcttcaatca gcggtttcat ataacgataa
     9601 aagaaagtaa gcagtaatac ttgaatatgg gcgccgtctg tatccgcatc ggtcatgatg
     9661 attactttgt cgtaattaca gtcttcgata gaaaactcag gaccgactcc tgcaccaatc
     9721 gtatagatca tcgtattgat ttcttcattt ttcaagatat cttgcatttt agctttttcg
     9781 gtattgatga cttttcctcg taaaggtaga attgcttgga actttctgtc tcttccttgt
     9841 ttagcagatc cgccagcaga atccccttcg actaaataca attcattttt cttagggttt
     9901 cgggattgtg cgggtgtcaa ttttcctgaa agaagagatt cgcctttttt acgcttcttc
     9961 ccatttcggc tttcttctct ggctttacgt gctgcttctc tcgcttctct ggctttgatt
    10021 gctttacgga ccaacatctg gctcatttcg ctattttcct gcaaataaaa gcccatttgt
    10081 tcactgatga cgttatctac tacggtacga gcaactggcg taccaagttt ttcttttgtt
    10141 tgcccttcaa attgcagaag attttcaggt acacggatag aaagtacagc agctaaccct
    10201 tctctgaaat cactaccttc cagattcttg tcccgttctt tcagcaaacc aacttttctc
    10261 gcatattcat tataagattt cgtcatcgcc gctttcatac cagcttcatg ggttccccca
    10321 tcttttgtcc gaacgttatt gacaaaagat aaaacatttt ctgaataacc gtcattgtat
    10381 tgataagcga cctctacttc gataccttct ttttctcctg aaaaataaac aactggtgtc
    10441 aatgtatctt tttcttcatt cagataatca acaaattctt tgattccttc ttcgtaatga
    10501 aaaatttcct tgactggttc ttctccacga agatcagaaa gagagatttt aacgcctttt
    10561 agaagaaagg cagattctct taaccgttcg gctaaaattt catatgaaaa tttcgttgta
    10621 ctgaatattg tatcatctgg taaaaaatgg acagtcgtcc cgtttgcttt ttttgttttt
    10681 cctatctttt tcaaagtacc gtcaggttta ccgccgtttt taaatttctg ctgatactct
    10741 acgccatctc taacgatcgt tacaattagc catttcgata aggcatttac cacactggct
    10801 cctacaccgt gcagtccacc agaagttttg taccctcctt gaccaaactt tccgccagca
    10861 tgcagcacag taaaaatcac ttcaactgtt ggtattccgg aaacatgcat tcctacaggc
    10921 atcccacgtc cgctatctgc aacagttata ctattgtctt catggatggt cacatcaatc
    10981 tcactgccat aacctgataa tgcttcgtca actgcattat cgacgatttc atagactaaa
    11041 tgatgcaagc ctcggctgtc agtggatcca atgtacatcc ctggtctttt tctaactgcc
    11101 tctaatcctt ccaatacttg gatggacgag tcattgtatt cgttgtttac tttttttgcc
    11161 aaggcaaacg ctccttcaat aatactttgc ggcaaaaccg caccacgatt atcatactag
    11221 aaaaggtcgt gaaaaaaaag agggtagacg agaaatcaat tttaattatc tcctaaccgc
    11281 tttttcgaca ttcccctcta tcgatcatct cttctttgtg gtcattttta tttttggttt
    11341 tctttcaact taccaagttc aatcttaata caacgatcca ttacgatatc tgtacgtcct
    11401 gcttttctta gcatctcagc tgcttctttg ttttcaatcc ctaattgtgc ccagaacact
    11461 ttagcatcag ttttcaaaaa atcacctgct atttcaggta aaaattcact ccgtctaaat
    11521 acatctacga tatcgattgg atcatcgatt tctgtcaaat tcgcatagac tttctcgcct
    11581 agaatttctt caccagccaa taatggattt actggtatga ttttatatcc attgtcttgc
    11641 aatacctgtg caaccatgta acttgttcgt tctggtttat tgctcaagcc tacgacggca
    11701 atattttttg cttcttccaa atagttgaaa atcgtttctt gtgttggatt ttttacagac
    11761 atttctgcca ccccttgctt ttctttttat tccatctatt atacttgtat ctttcatttt
    11821 ttcaattgtt cctgttacct attcatgcta aaataaagga tagttttcaa cttgaaagag
    11881 ggtaaaaaat gaaattgatc ttattactta ttttagcata tcttttaggt tctattcctt
    11941 ccggtgtctg gattggcaaa ttgtttttca aaaaagatat ccgacaacac gggagcggta
    12001 atactggaac gaccaataca tttcgtgtat taggcaaaac agccgggatt gttgtcctgc
    12061 ttatggatat tttaaaaggt acattggcta cttgtttgcc gatgatcttc cacctttctg
    12121 ggattaaccc tctttggttt ggggtatgcg ccattcttgg ccatacattt ccgatttttg
    12181 ctgggttcaa aggaggaaaa gctgttgcga caagtgcagg aatgctttta ggatttaatc
    12241 ctgtattttt tgtttattct tgtatcatct ttatcgttag tttattttgt acaagtatgg
    12301 tgagtttgag cagtatgatc agtgcagtat tgatcacttt atctacgatc gtccttcctt
    12361 atgtagctcc tgtgattttg gctaagccca attggttact gacgatcatt gcctttttag
    12421 tttcttcctt tatcttttat cggcataaag acaatatcaa gcgtatcaga aaccatactg
    12481 aaagtcgaat cccgtttggt ttgaatagca gcaagaaaaa atcctgatac tttttcattc
    12541 caaagagata aaacgggagt gtgacaaatc gatgtcacac tcccttatta cttacaggat
    12601 agctttttta cacaccttgg taaacggtgt tccctgctaa cttctcggtc acaacctctt
    12661 acttcactgt gatggaatat tttgtcttga agatttcatg ggcacctagt tgattgatcc
    12721 ctactttttc catcaagttt ccactactgt tcaccgtgtc agcgattccg caccacggtt
    12781 cgatacaaac gaaaggcgct tcttttggat aaggagacca gatccctaca taaggcatat
    12841 ctttatagct taaagttacg ctatgggaag atttttcact tcgaatggcg tacgcattta
    12901 gaccccttgt ttcaaaaacc aaggcatctt gctcaaaaag ttcacgattc aaagccagat
    12961 tcgtatttgt ctgacctaac gtcttttgat ccatatcgat gaatgggcct ttcaatggta
    13021 ggcgtaatct tgatttcaat ggagaaaatg ccagatagaa atcttcaaat gacaatcctt
    13081 cttcaagagg tacattgaaa gcaggatgtc caccgataga aaagaacatt tctttttcac
    13141 cggtatttgc cacttgataa tgaaccgtga tgccgtctcc tcctaattca tagctgatca
    13201 ctaattcaaa atcaaatgga tacttttcta atgtctccgg cgtactcctt aagatgaaac
    13261 tagccatttc ttgtccatgt tcaatgactt caaaatccat atcccgagca aatccgtgtt
    13321 gccccatttg atatgttttc ccttgataaa catactgatc atcttttaaa cgtccaacaa
    13381 aagggaataa aaccggtgca tgtctgcccc aatacgctgg atcaccttgc cagatatact
    13441 ctaaaccatg ttcttttgat ttcagactga tcaattccgc accgtgttca gcgatttcgg
    13501 cgatcagctg gtcattttga ataataacag tcattgccat tcctccaact tcgacttact
    13561 ttttaaaatg ggaaggctgg aacaaacatt tctatctgcc ccagtcccct tacttctaat
    13621 aactgtgttt tgctaactcg tctttaaaac gggaattcaa tacttttaaa taagttccct
    13681 tcatgcctaa cgaacgagat tcaatgattc cagctgattc caattttcgt aaagcattga
    13741 cgatcacaga acgagtgatg ccgatactat cggcaatatt agaagcagtc agacgacctt
    13801 catctccatc caatgcttca aagatagctt gtacagcctt caattcgcta taagataatg
    13861 tgttgattgc catttggaca gccgttgcac tccgtacatc tgcttcgatg cttcgagact
    13921 tctgataaag gatctgcata ccgacaaccg tagctccata ttctgctaag actagatcgt
    13981 catctgcaaa cgattgttcc atacgagata agataatcgt acccaaacgt tcgccagctc
    14041 caaagatcgg aacgacagtc gtcagaccaa atggatattt ctctcttgac tctaccggaa
    14101 atgcagtaat atcactagta attgggatat tagcttcagt tttcatcaac tgtgccactg
    14161 tatcagtata agattcagga aattgtttct caacaaacat tgttttgatt cgttcattgt
    14221 tcacatcgtg acgttcatta taacctagta atactccgtc acttcctatg atataggtgt
    14281 tgctgtcaag aataccacct agtgtgacag ccattttatc gtaaggcaaa tctgcttgca
    14341 tgtcaaaagt attcttttgc tgcaaaagtg tgtttatctt gcgggttttc gttagcaagt
    14401 ctgtcattgt cgtcctccga ttttttataa aatatagcga cttaaatctt catttgccgc
    14461 aattgagcca agtttgtcat ccacatatgc ttctgtgatc gttatttctc ccatttgcat
    14521 atctggtgct tcaaataata gatcttctag tagtttttcc aaaatggtat gcagtctgcg
    14581 tgcgccgata ttatctgtgt cacggttgac atcgaaagcg atttgagcga tccgttcaat
    14641 tgcttctttt gtaaacgtaa ctgacacatt ttctgtaccg atcaacgcga cgtattgttt
    14701 tactaaagca ttatttggtt cggtcagaat cttcacaaaa tcttcagctg tcaaatcatc
    14761 taattctacc cgtatcggga agcgtccttg aagttctgga attagatcac ttggtttaga
    14821 caaatggaaa gctcctgaag caatgaatag gacgtgatct gtttggatga tcccgtattt
    14881 tgtgtttact tgtgaacctt cgacaattgg caatatgtct ctttggacgc cttctctgga
    14941 tacttctcca gattgctgtg atttagaagt gatcttatcg atttcatcga taaaaataat
    15001 tccactttct tccgctaatt tgattgcctc actatggata tcagcatcct tgactaattt
    15061 tgctgattct tccttgatca ataactctaa agcttctttg actgttaccg tccgttccac
    15121 ttttttcttc ggtgtcaaag cacctagagt ttcatttagg tcgatcccca tttgctccaa
    15181 tccattgttc atcattggtg caggtttctt ttggtcttca acttcaatgg ttacttcacg
    15241 actatccagt aaaccctttt gtagttgttc caagactgta cggcgattga ttcgaatatc
    15301 ttctgtcact tcttcttgcg gttcagcggt attttgctga ctagcattaa aaagattcat
    15361 catttgttca aattgattag tggattgttt ctgttctttt ttgataccag gtaccaagat
    15421 ttttaccaaa cgtttttctg catttttttt agcacttgtg tatacacggc tgtactgctc
    15481 tttttctacg atttggatcg cattttctac taaatcccga accattgatt ctacatctct
    15541 tccgacataa ccgacttcag taaattttgt tgcttcgact ttgacaaatg gtgcattgac
    15601 gatttttgcc aaacgtcgtg cgatttctgt tttaccgacc cctgtaggtc cgatcatcag
    15661 catatttttt ggtgtaatat cttgttgcat tttttcatct aactgcaaac ggcgatagcg
    15721 gttacgcaaa gcaacggcaa cagatttttt tgctgcgtgc tgtcctacga tatattcatc
    15781 taattcactt acgatttgtc ttggtgtttg ttttaattca tacatatcta ctcacctctt
    15841 acagttcttc tacgataata ttatgattag taaagataca gatatctgcg gcaatattta
    15901 acgcattttc tgcaatttct ttcgcactca tgtcgttttg agcatagttt ttcattgctc
    15961 gggctgcagc caacgcaaaa ttccctcctg atccgatagc taagatacca tcatctggtg
    16021 tgataacttc tcctgtacct gaaacaagaa gcatttcttt atcattcatt acgatcagca
    16081 tcgcttcaag tttttgcata gattgctgtg tccgccattc ttgagctaat tcgacagctg
    16141 cgcgttgtag attcccgtta tattcgttta gtttgccttc aaatttttct tctaaagtaa
    16201 acgcatctgc aacacttccg gcgaaaccta caacaacttc tccattgtag atacgtcgta
    16261 ctttctttgc agtgcctttc atgacaacag attcacccat tgttacttgg ccatctccag
    16321 ccatagcaaa tttcccgtct ttttcgacgg cacagatagt ggttgaatga aatggtgatt
    16381 cgaccatgtt tgtttcctcc taaatttgtt cttttcctta tttttacgct cttggatgga
    16441 aagaacgata gtttttttgt aaactttctt ttgttacatg ggcatagatc tgggtagtcg
    16501 ataaattggc atgtcctagc aattcttgga ctgtccgcaa atctgcccca ttatttaata
    16561 aatgagtagc aaatgtatgg cgcagcatgt gaggatggat atcactatcc aaactgcttt
    16621 ttttgatcat ttgattcaag acatattcaa ttccagtagg cgtgattggt tttccatgat
    16681 gattcacaaa aacgatatca tgctcttcgt gatattttgt catcaatacc tggcggcctt
    16741 ccctgaaata ttcttgcaga gcatcttgag caaaagaacc tagaggagca tatctctctt
    16801 tgttcccttt accatggatc aacatgacct ctgaatcaaa atcaatgtct gataccgaca
    16861 aactgctaca ctcacttaaa cggatgcctg acccgtaaag gacttccagt aaagcgcggt
    16921 tcctcaaatc caaaggacta tctcctgcta cgctgtcaaa aagtgcttgc atttcatttt
    16981 cataaaaaaa acgtggcagt ctcaaatttt tctttttgag atgaacgtaa gaaaatggat
    17041 tttcttttat cacttcttct ttgagaagat actgataaaa cgaacgtagg ctggctatct
    17101 tgcgactgat agaatttctg ctatagctag gacgatgatc agctaatgca ctcaaataga
    17161 cacgaaccgt aaaatattcc gccttcagca gatcagcgtc tccagattcc tccaaaaaat
    17221 gtttgaaatg gagaatgtct tcttcatatg cttctttcgt tttttctgaa tatcctcttt
    17281 cgacaatcaa ataacgcaaa aatttttgtt cccacgtggc ttctgtcatt ttctcacctt
    17341 ccttttgtcc cagagaataa tttagcacag cgtttataaa aagacaaata aattgtcaga
    17401 atttaaccaa tatttctaag tttgttcaca attggcgcaa ttcctcacat tttgtttgag
    17461 aaattcttaa gaaaaacgca agaaaaagag cgagactatc cgtctcaccc tcttcatgga
    17521 gtttccttac tcatttggga ttgcagtttt ttctaattct gtttttattg cctcattagc
    17581 ttttaaagct cgttgtgcaa ttgcttcata tcttgctttt ttgtctcgaa tgcgctctgg
    17641 cagttcagga aacagaccat agttggcatt catcggttgg aaatgttttc cttccgcatg
    17701 agtaatgtag taagccatac ttccaatagc tgtttcttgt ggaaactcga ctggttccat
    17761 ttcttttgct aagcgtgcgg cgttgattcc agcaagtagt ccgctcgctg cactttcaac
    17821 atagccttct acaccagtca tctgacctgc aaagaacaga tcttcgcgtt tcgttgattg
    17881 gtaagtcggt ttcagcaatt ctggcgaatt cataaagctg tttcggtgca tcacaccata
    17941 gcgaacgaat tcagcatttt ctaacccagg aatcatccgg aaaacacgtt tttgctcgcc
    18001 ccatttcaag tgagtctgaa agcccacgat gttgtataaa gaagctgcag cattgtcttg
    18061 acgcagctgg ataacagcat aaggacgttt gccagtttta ggatcttcca atccaactgg
    18121 tttcataggt ccgaaaagca tcgttttgaa tcctcgctgt gccattactt caattggcat
    18181 acatccttca aaatattttt ctttctcaaa agatttaagt tctgccactt cggcattgat
    18241 tagtgcctca tggaaagctt tgaattcctc ttctgtcatc ggacagttca agtaagctgc
    18301 ttctcctttg tcatagcggg acttcaaata tactttatcc atatcgatcg tagctttgtc
    18361 tacgattggt gcagctgcat catagaaata aaaaccatct gaaccattga atgctttgat
    18421 gctttctgct aatggatgag aagtcaaagg tcctgttgct actacggtga ttccttccgg
    18481 tatttcagta atttcttcgt ttatcactgt tactaatgga tgctctttga cttttcgggt
    18541 aattgtttca gaaaaagaat cgcggtcaac agctaaggca ccaccagctg gcactgctgt
    18601 ttcatctgcc gaagtgatta ctaccgaatt caatcggcgc atttcttctt tcaataaccc
    18661 tactgcatta gccaaactgt ttcctcttaa tgaattggaa catactagtt cagcaaaatt
    18721 acttgtgtga tgcgcctcag tcgatttgac tggacgcatt tcatataaac ggacagggac
    18781 tcctgcttgt gcgatctgcc aagcagcttc acttccggca agccctgctc caacaacatt
    18841 taccattttc aatgaaagaa cctcttttct attatttttg gatgtcttct tcgtaatctc
    18901 catttacaca gactacttgt ttaccgcctt tgactttctt ctcgaccaga tatccgtcac
    18961 attttggaca atttctgccc actggtttat cccaagaagt gaattcacat tctgggaaac
    19021 gggaacaacc atagaatagt ctgtttttct tagattttcg ttcaatgact tgtccttcat
    19081 gacatactgg gcaagtcaca ccgatttctt ttacgattgg tttcgtattc cgacaatcag
    19141 gaaaattact gcaagcataa aacttcccat atttccctaa ttttatcacc atcggatgtc
    19201 cacagacatc acaatcgaat cctgctggtt catctttgat ttgaattttt tcgatttttt
    19261 cttccgcatt cgtcagttct ttttcaaacg gtttatagaa acgatcaacc actttgaccc
    19321 acgcttccgt gccttcttca attttatcca aatctccttc catttctgct gtgaaatgga
    19381 tatcgacgat ttgagggaag aattcacaga ttaatgaatt gacaatttct cctagttcag
    19441 tcggctcaaa tcttttttgt gtcaatttta catagtagcg tctttggatc gtatcaagtg
    19501 ttggtgcata agtggaaggt cgccctaccc cgttttcttc tagtgcacga atcaacgtag
    19561 cctcactaaa acgtgctggt ggttgagtaa aatgttgttt tggttcaata tcaacagatt
    19621 ggactacatc gccttcttca agttctggaa gaatattttc tttctcttct ttgccgtcat
    19681 ctctaccttc cacatacact tgcatgaatc ctttgaattt gatttttgaa ccgttcgcaa
    19741 tgaagatgac accattttgt tctaatgtca ctttcattgt atctaaaata gctggtgtca
    19801 tctgactagc cacaaaacgt gaccagatca atgtataaag tttcaattga tctttatcta
    19861 aatattgttt gagttcatct ggtgtacgaa gcacactcgt tggacgcaca gcttcatggg
    19921 catcttgtgc gccttgagca tttttgatct ttcggtgatc atgtctagaa aattcttttc
    19981 catacgtatc ttcaataaat gctgcggcct ctgcttttgc tgtatcggca atccgggtag
    20041 aatcagtacg catgtatgta atcaagccga ctgttccttg tttacccaaa gtgattcctt
    20101 catataattg ctgtgctacc atcatggttt ttcttgttcg aaagtttaac ttacgggcag
    20161 cttcttgttg caggctactt gtagtgaaag gattcgcagg gtttcttttg cgctctttct
    20221 tttctacttt tttaacttcg aagtctgttc catcaattct agcagtgact tcttttacac
    20281 tttgtgcatc tggtaatttc tttttcttac cgtcgattcc ccagaaattt gctttgaatt
    20341 tcttgcgagc ttttttaaaa ttaccgtcga tgctccaata ttcttctgga acaaattctc
    20401 ggatctcttt ttcacggtca ataataattt ttaaagcgac agattggaca cgtcctgcac
    20461 ttaacccttt cttgacctta cgccagagaa taggactgat cgaataacca acgattctgt
    20521 ctaaggtacg acgagcttgc tgtgcatcta ctaaatctac atcgatcgtt cttggttcct
    20581 taaaagctgc tttgactgcg tcttttgtga tttcattgaa gaccacccga tttttttctt
    20641 tcagatccaa cccaagtagg tacgctaaat gccaagcaat cgcttctcct tctctatccg
    20701 ggtcacttgc caagtaaact ttttgagctt tttttgctgc ggctttcagg cttttgatga
    20761 catcgccttt tccgcgtata gaaatataat ggggttcata attgttttca aaatcgatac
    20821 ccattttact tttcggtaaa tctcttatat ggccaacact ggccattact ttatagtttc
    20881 gtccaagata tttctcaatt gtcttggctt ttgcaggtga ttctacaatc actaaatatt
    20941 tatacgccat tactgaatgg actcctttcc attgtcgtgt tcctactatt aaaagaggat
    21001 tcatacaaat cgtagctaat catatccatt cagaaaacgt ttgtcaagaa aagcaaatga
    21061 aaagtatggt gttattcttt aaattctgcc aagatatctt cacccgaaac cgtgcaaact
    21121 gcaccgtctt ggatcaaatg atgacatccg gtagaatgtt ctgttaaaat atttcctgga
    21181 acagaaaaaa ctgttcttcc aagatccaat gcttgctgtg ctgttatcag agagccgctt
    21241 tttttcttgg cttcaaagac acatgttcct ttagacaaag ctgcgataat ccgatttctc
    21301 attggaaaac aatgcttttt cgggccggat tgaggaggat attcactcaa aagaagatga
    21361 tcatttgcaa tcacctcttg caagtcacga ttttcagcag gatagacttg ctctatccct
    21421 gttccaatga ctgcgattgt ccgtccacca tgagagattg ctgcttggtg accgtatgta
    21481 tctatccctt tcgcgcagcc actgacgatc acatatccag cttctatcac tggttttagt
    21541 aaataataaa ctgttttttt ggctaaagca gaagtgtgtc ttccaccaac aacgcttaac
    21601 atgttacaag aaagtaaaga tagatcacca ttataaaaca gtacaattgg cggataggtc
    21661 aagtgaagca gctgaggggg atatttggga tcaaagcaag tgataaacga ctgtttcgat
    21721 tggattttgc cccagttagc gtgaatttcc tgccaacttt tgcgaaacag ctcccggtgt
    21781 ttggttattt tacctatctt aatgatgtct tcaaatccga tttctttata aggatactgc
    21841 atcatccagt tagcaagtgc ccattttcct tgactactga ttccttttgt ataagcaagt
    21901 ttcaataaat tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat
    21961 aagatacgta aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt
    22021 acgatggatt ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata
    22081 acctgcatta tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg
    22141 gtctcgatat actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc
    22201 tttgattatt ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata
    22261 gcttggtaca atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata
    22321 aatattcaca tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt
    22381 ttcttgaact tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt
    22441 cagccctaat atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc
    22501 taatggtcct cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc
    22561 ttgcttttca aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa
    22621 tcgtcgttca aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga
    22681 ttctagtgta ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat
    22741 ggattcagtc atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa
    22801 gttttccttg acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat
    22861 acccacgttt tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga
    22921 ttgataaacc ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc
    22981 cgtaaatcgc taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta
    23041 gttttttgcc gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca
    23101 attccaactc attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc
    23161 ctgtctgtgc aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg
    23221 gaatacctaa gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg
    23281 cccgtttttc tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt
    23341 cttgtgccgt gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct
    23401 ttgtctgttg tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct
    23461 gaatgatctg atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt
    23521 caaatacgat gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat
    23581 gtcccggaaa ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc
    23641 cggctttact tgtttttttc tttttcaata taaattcact tttttattat aaagaaattc
    23701 catctattag ttaagtgaaa gtgaaccagt cctcattcta tcacatcttc acttttttga
    23761 tgatgcaaat ttttattttg gtttctatat atgaatctaa atttttgaca gcgtttccta
    23821 aataagagat gatagacgaa caaaacagaa aggagacgaa acaaatgaat agtatggttt
    23881 ttgttaattt tcctgttgct gatgtcaatc gttccgttgc tttttatgaa aagctgggtt
    23941 ttaaaaaaaa tgaagaattt tctactgaag aagctggttc aatggtatgg gatgacactt
    24001 tttggataat gcttctctct catgattttt ataaaaaatt tataa
//
LOCUS       DAYYNG010000028        23818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000028 DAYYNG010000000
VERSION     DAYYNG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.028"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..779)
                     /locus_tag="ACHBFA_000910"
     CDS             complement(45..779)
                     /locus_tag="ACHBFA_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9394421.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="ACHBFA_000911"
     CDS             1106..1516
                     /locus_tag="ACHBFA_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM9394422.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="ACHBFA_000912"
     CDS             1509..2210
                     /locus_tag="ACHBFA_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM9394423.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="ACHBFA_000913"
     CDS             2210..3796
                     /locus_tag="ACHBFA_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394424.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="ACHBFA_000914"
     CDS             3815..4036
                     /locus_tag="ACHBFA_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9394425.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="ACHBFA_000915"
     CDS             4033..4794
                     /locus_tag="ACHBFA_000915"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9394426.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="ACHBFA_000916"
     CDS             5174..5683
                     /locus_tag="ACHBFA_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9394427.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="ACHBFA_000917"
     CDS             complement(5758..6297)
                     /locus_tag="ACHBFA_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9394428.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="ACHBFA_000918"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="ACHBFA_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9394429.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7310..8161
                     /locus_tag="ACHBFA_000919"
     CDS             7310..8161
                     /locus_tag="ACHBFA_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9394430.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8189..8824
                     /locus_tag="ACHBFA_000920"
     CDS             8189..8824
                     /locus_tag="ACHBFA_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM9394431.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="ACHBFA_000921"
     CDS             8844..9617
                     /locus_tag="ACHBFA_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9394432.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="ACHBFA_000922"
     CDS             10025..10822
                     /locus_tag="ACHBFA_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM9394433.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="ACHBFA_000923"
     CDS             10800..11213
                     /locus_tag="ACHBFA_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9394434.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="ACHBFA_000924"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="ACHBFA_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9394435.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="ACHBFA_000925"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="ACHBFA_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9394436.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="ACHBFA_000926"
     CDS             15990..16550
                     /locus_tag="ACHBFA_000926"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM9394437.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVEFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="ACHBFA_000927"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="ACHBFA_000927"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9394438.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="ACHBFA_000928"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="ACHBFA_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9394439.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="ACHBFA_000929"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="ACHBFA_000929"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9394440.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="ACHBFA_000930"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="ACHBFA_000930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9394441.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="ACHBFA_000931"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="ACHBFA_000931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9394442.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="ACHBFA_000932"
     CDS             21897..22475
                     /locus_tag="ACHBFA_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9394443.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="ACHBFA_000933"
     CDS             22527..23009
                     /locus_tag="ACHBFA_000933"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9394444.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="ACHBFA_000934"
     CDS             23126..23500
                     /locus_tag="ACHBFA_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394445.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7775 a         4284 c         5114 g         6645 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 tagaatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagt
//
LOCUS       DAYYNG010000029        23508 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000029 DAYYNG010000000
VERSION     DAYYNG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.029"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHBFA_000935"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACHBFA_000935"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9394446.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACHBFA_000936"
     CDS             complement(1173..1868)
                     /locus_tag="ACHBFA_000936"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9394447.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMKKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACHBFA_000937"
     CDS             complement(1881..2597)
                     /locus_tag="ACHBFA_000937"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9394448.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACHBFA_000938"
     CDS             2927..3532
                     /locus_tag="ACHBFA_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM9394449.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACHBFA_000939"
     CDS             3857..4180
                     /locus_tag="ACHBFA_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9394450.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACHBFA_000940"
     CDS             4362..5732
                     /locus_tag="ACHBFA_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9394451.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACHBFA_000941"
     CDS             complement(5862..7163)
                     /locus_tag="ACHBFA_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9394452.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACHBFA_000942"
     CDS             complement(7393..9384)
                     /locus_tag="ACHBFA_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM9394453.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACHBFA_000943"
     CDS             complement(9528..9794)
                     /locus_tag="ACHBFA_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9394454.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHBFA_000944"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACHBFA_000944"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9394455.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHBFA_000945"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACHBFA_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9394456.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACHBFA_000946"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACHBFA_000946"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9394457.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACHBFA_000947"
     CDS             complement(13954..14475)
                     /locus_tag="ACHBFA_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9394458.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACHBFA_000948"
     CDS             complement(14478..15320)
                     /locus_tag="ACHBFA_000948"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9394459.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACHBFA_000949"
     CDS             complement(15313..16038)
                     /locus_tag="ACHBFA_000949"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM9394460.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACHBFA_000950"
     CDS             16214..16510
                     /locus_tag="ACHBFA_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394461.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACHBFA_000951"
     CDS             17078..18670
                     /locus_tag="ACHBFA_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9394462.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACHBFA_000952"
     CDS             18683..19423
                     /locus_tag="ACHBFA_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9394463.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19591..20940
                     /locus_tag="ACHBFA_000953"
     CDS             19591..20940
                     /locus_tag="ACHBFA_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9394464.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21068..21658)
                     /locus_tag="ACHBFA_000954"
     CDS             complement(21068..21658)
                     /locus_tag="ACHBFA_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9394465.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21805..23157
                     /gene="brnQ"
                     /locus_tag="ACHBFA_000955"
     CDS             21805..23157
                     /gene="brnQ"
                     /locus_tag="ACHBFA_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9394466.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6723 a         4739 c         4485 g         7561 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttt catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaaat tttggaatct tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa
    19561 ttaaactttt ttagttagga tgatttaaaa atgaatgaag agcaagaact gcagcgtgga
    19621 ctgaaaaata gacatgtcca actgatttct attggcggag cgattggaac ggggttgttt
    19681 ttaggttccg gaaagtctat ccagctagct ggcccttcga tattgctcgc ctatcttatt
    19741 actggatgca tctgtttttt cattatgcgt gcgttaggcg aacttctgtt atcaaacacc
    19801 aataaccatt cttttctgga ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc
    19861 actggctgga cgtattggtt ctgctgggta tcgattgcaa tggccgattt aacagccatc
    19921 ggaatgtatg tacgctactg ggcgcctggt gttcctcagt ggcttcctga actgattgca
    19981 ttaggtcttc ttctttgctt gaatatggtg gctgtctcat tgtttggtga gttagagttt
    20041 tggtttgcat tgatcaaagt tgtagcgatc attgccttta tcctcgtagg agcttatatg
    20101 atcctcaccc actatacgac agatattggc acagccagta tcacgaatct atggtctcat
    20161 ggcggattct ttccaactgg tgcaaaagga tttatcttag ctttccaaat ggtaaccttt
    20221 gcttttgctg ggatagaatt agttggatta gtcgctggag aaacagaaaa tcctgaaaaa
    20281 gtgttgccgg aagctatcaa taatattcct atcaggatca tcttatttta cttaggatct
    20341 ctttttgtca ttatggcaat ctatccatgg aacagcttga atccagataa cagtccattt
    20401 gtagaagtat tttcggaaat tgggatcacg attgctgctt ccttgatcaa tttagtcgta
    20461 ttaagtgcag cagcttctgc ctgcaacagt gcaatatata gtacaggacg tatgcttcgt
    20521 tctttagcgc aagaaggcag tgcccctaaa aaattcaaaa agttaacaac acatcatgtg
    20581 ccagggaatg ctttgacatt ttctactatc gtcattttca tttctgttat cttgaattac
    20641 gtgatgccta gtgaagtatt cacactagta agcagtattg ccactacttg tttcttgttt
    20701 atctggagta tgcttgtcta cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa
    20761 gctcattcat ttaaaatgcc gttgtaccca tttagtaatt atctggtatt tctttacatg
    20821 gcctttgtgt gtgtcgtctt gttcctagga aaagatacga gaatcgcttt attactaaca
    20881 cctgtgtggt tcttgcttct attactaatc tatcatatga aatatgaacg taagaagtaa
    20941 gaaagtagtt gtgacagaag tggtcagctt cgagaaataa gaaggaattt acgaaaattg
    21001 cttcttaaat ttttgtgaaa tttcggctta tttcttggag ataaaagctt ctgtcccagc
    21061 ctcttttcta tttccccgga aacaaccgct gataataggc atctgctttt ttataggaaa
    21121 aaatccaaat accagataca aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga
    21181 caccggcaat caagcataag ttaatcatca tggagataag acttcgacgg tcctttgaca
    21241 aaactctcac gacatgggaa tctggtttat ttgctttcat gatttgcctc atcaagatac
    21301 caaaagaaaa actccacatc gtgtatacta agacataagc catctcaggc caaaaagctt
    21361 cagggttacg cccgacccaa ctagtaacag aaggacataa agtcagccaa aagagcaaaa
    21421 agatattcga ccagaccact cggtaattaa ttcgttctgc catctgaaat atacgatgat
    21481 ggttcaccca gatcaccgca acaaatataa agctgatggt ataagctaat aatgtatttc
    21541 ggatgaccca taaagaatga aagctagatg tattagggat cttgatctct aatacaataa
    21601 ctctcaaaac aatggctacc actccatcag taaatgcttc taatctcgac tttgtcacga
    21661 tgattcctcc tcagatgatt cagcgctttt attttatcac ttacgttatt aaataaccat
    21721 cgttttatat agggaatttt ctgaaaattg ttgcatattt ttcgaaattt tgttactttt
    21781 aaaaaaaccg taaaggagtt atgtatgaca aaaaaattat catttaaaga ctatcttttt
    21841 ataggctcca tgcttttcgg tctatttttt ggtgctggaa atcttatctt tcccgttcat
    21901 ttgggtcaag aagcaggcgc tgctaccttt tgggcgaatc taggtttctt ggttaccggt
    21961 atcggtcttc ccttcttagg ggtgattgcc attggtgtat cgaaaagctc aggtgtatat
    22021 gaattagcaa aacggataaa tcaaacatat gcgatggttt ttaccatctt gctttactta
    22081 gttatcggac cgttttttgc tttacctcgc cttgcaacca catctttcga gattggttta
    22141 gcaccattta tcgatcctag cagacagacg ctatatctag caattttcag tattgtattt
    22201 tttgtgctgg cttggtggtt ttcaagaaaa ccgacaaaga tattagatta tgttggaaag
    22261 ttcctgaatc ctgcattttt ggttttatta ggtgctttgc ttctccttgc ttttttgaat
    22321 ccactaggtt ctgtaaatca agcgcctatt cagccaaatt atcaagagca tgcttttttc
    22381 acagggttta ctcaaggata taacacatta gatgcgcttg ctgctttagc atttgggatc
    22441 atcattgtca caacaatcca aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac
    22501 acgatcaaat ctggtgcgat cagcattata ttgatgggaa tcatttatac tttgctcgct
    22561 tatttaggtg ctatgagctt aggtagcttt gctctaagtg aaaatggcgg gatcacgctg
    22621 gctcagattg ctgatcatta tttaggtact tatggcagta tcttattagc atttatcgtt
    22681 atattagctt gcttgaagac cggtattggt ttgattacag cttttgcaga aacattcaca
    22741 gatttgtttc cgcaaagaaa ttacgcattc tttgttgcac ttgctagtat ccttccttgt
    22801 ttagcggcaa acgtaggctt gacgaatatc attcaatttt cgttgcctgt tttgatgttc
    22861 atctatccat tagcaatgac actgatttta ctagtgttag ttggtccttt gtttaagcag
    22921 cgtacggctg tttatcgaat gactacttat tttacattga ttgcttcgat ttttgatggg
    22981 ttgaacgctt gcccagaaac tatcaaacaa acacctattg ttcaaaatct tttatatgtt
    23041 gcggaaagct acttgccgtt tttcaaattg gggatggggt ggatcgtacc agcagttatc
    23101 ggttttgtta tcggactgat ttggagtttc gctaagaaag aagaagtagc agattgataa
    23161 aagaggttgt gaaagagacg ttcagccttg agcaataaaa agtaattttg aaaaatggct
    23221 tctcacattt ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgttta
    23281 ttgagaggtc gtgaaaaagc cgcttagttt caagtattaa gaaatatttt gtgaaaatgg
    23341 cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt gaacaccgtt
    23401 tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa gtagctccat
    23461 atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaa
//
LOCUS       DAYYNG010000030        22852 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000030 DAYYNG010000000
VERSION     DAYYNG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.030"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..517
                     /gene="relB"
                     /locus_tag="ACHBFA_000956"
     CDS             194..517
                     /gene="relB"
                     /locus_tag="ACHBFA_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9394467.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            837..1064
                     /locus_tag="ACHBFA_000957"
     CDS             837..1064
                     /locus_tag="ACHBFA_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM9394468.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1080..2246
                     /gene="hemW"
                     /locus_tag="ACHBFA_000958"
     CDS             1080..2246
                     /gene="hemW"
                     /locus_tag="ACHBFA_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9394469.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2481..3524
                     /gene="hrcA"
                     /locus_tag="ACHBFA_000959"
     CDS             2481..3524
                     /gene="hrcA"
                     /locus_tag="ACHBFA_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9394470.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3618..4181
                     /gene="grpE"
                     /locus_tag="ACHBFA_000960"
     CDS             3618..4181
                     /gene="grpE"
                     /locus_tag="ACHBFA_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9394471.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4232..6061
                     /gene="dnaK"
                     /locus_tag="ACHBFA_000961"
     CDS             4232..6061
                     /gene="dnaK"
                     /locus_tag="ACHBFA_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9394472.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6212..7378
                     /gene="dnaJ"
                     /locus_tag="ACHBFA_000962"
     CDS             6212..7378
                     /gene="dnaJ"
                     /locus_tag="ACHBFA_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9394473.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7746..8483)
                     /locus_tag="ACHBFA_000963"
     CDS             complement(7746..8483)
                     /locus_tag="ACHBFA_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9394474.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8485..9987)
                     /locus_tag="ACHBFA_000964"
     CDS             complement(8485..9987)
                     /locus_tag="ACHBFA_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9394475.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            10246..12240
                     /gene="uvrB"
                     /locus_tag="ACHBFA_000965"
     CDS             10246..12240
                     /gene="uvrB"
                     /locus_tag="ACHBFA_000965"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9394476.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            12250..15066
                     /gene="uvrA"
                     /locus_tag="ACHBFA_000966"
     CDS             12250..15066
                     /gene="uvrA"
                     /locus_tag="ACHBFA_000966"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9394477.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            15329..16594
                     /locus_tag="ACHBFA_000967"
     CDS             15329..16594
                     /locus_tag="ACHBFA_000967"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM9394478.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            16598..17329
                     /locus_tag="ACHBFA_000968"
     CDS             16598..17329
                     /locus_tag="ACHBFA_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM9394479.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            17464..18348
                     /gene="rapZ"
                     /locus_tag="ACHBFA_000969"
     CDS             17464..18348
                     /gene="rapZ"
                     /locus_tag="ACHBFA_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9394480.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            18345..19343
                     /gene="yvcK"
                     /locus_tag="ACHBFA_000970"
     CDS             18345..19343
                     /gene="yvcK"
                     /locus_tag="ACHBFA_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM9394481.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            19377..20312
                     /gene="whiA"
                     /locus_tag="ACHBFA_000971"
     CDS             19377..20312
                     /gene="whiA"
                     /locus_tag="ACHBFA_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9394482.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20880..21536
                     /locus_tag="ACHBFA_000972"
     CDS             20880..21536
                     /locus_tag="ACHBFA_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394483.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21715..22280
                     /locus_tag="ACHBFA_000973"
                     /pseudo
     CDS             21715..22280
                     /locus_tag="ACHBFA_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7571 a         3843 c         4988 g         6450 t
ORIGIN      
        1 aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa aaagtagttg
       61 gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg atcatttact
      121 acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt tctaaaatca
      181 catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga ttacggttcg
      241 tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg ggattacttt
      301 atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca atcgtcaaac
      361 ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat tcatggaaga
      421 atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg cccgaaattc
      481 agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa tcaattcgtt
      541 gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac tgtttatttt
      601 tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat ttgactagat
      661 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
      721 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
      781 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
      841 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
      901 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
      961 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
     1021 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
     1081 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
     1141 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
     1201 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
     1261 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
     1321 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
     1381 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
     1441 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
     1501 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
     1561 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
     1621 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     1681 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     1741 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     1801 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     1861 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     1921 atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg
     1981 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     2041 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     2101 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     2161 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     2221 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     2281 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     2341 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     2401 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     2461 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     2521 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     2581 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     2641 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     2701 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     2761 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     2821 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     2881 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     2941 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     3001 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     3061 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     3121 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     3181 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     3241 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     3301 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     3361 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     3421 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     3481 aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     3541 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     3601 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     3661 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     3721 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat
     3781 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     3841 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     3901 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     3961 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     4021 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     4081 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     4141 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     4201 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     4261 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     4321 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     4381 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     4441 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     4501 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     4561 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     4621 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     4681 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     4741 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     4801 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     4861 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     4921 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     4981 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     5041 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     5101 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     5161 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     5221 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     5281 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     5341 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     5401 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     5461 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     5521 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     5581 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     5641 cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg tgaaagatgc
     5701 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     5761 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     5821 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     5881 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     5941 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     6001 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     6061 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     6121 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     6181 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     6241 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     6301 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     6361 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     6421 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     6481 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     6541 aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     6601 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     6661 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     6721 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     6781 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     6841 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     6901 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     6961 aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa gtgatatctt
     7021 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     7081 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     7141 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     7201 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     7261 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     7321 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     7381 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     7441 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg
     7501 tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa
     7561 ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg
     7621 gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta
     7681 agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcgaccggg catttttggt
     7741 ttttattaaa tatttaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt
     7801 tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca
     7861 cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct
     7921 tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca
     7981 gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc
     8041 acacgctgct tttgtccacc ggaaagcatt tcaggataca tatctttttt atcttctaga
     8101 ccaactgtct gcagcaattt gattgctttt ttctctgctt cttctctaga ttctttcttc
     8161 aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag
     8221 tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc
     8281 aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa
     8341 cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg
     8401 ttgatggaca cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc
     8461 tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt
     8521 tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact
     8581 ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc
     8641 ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat
     8701 gatgcgtatc gcttgcggca aaatgatttt ttgcattgtc cgattataag aaagacctaa
     8761 acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc
     8821 tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat
     8881 cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg
     8941 aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc
     9001 tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt
     9061 taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc
     9121 aagaaatgtt gattcatcag cagtggaaga ctcactggaa gtttctgaac catccgctac
     9181 atattcttta ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt
     9241 gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc
     9301 agattctttt ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac
     9361 cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc
     9421 agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc
     9481 agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt
     9541 gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga
     9601 aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc
     9661 ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt
     9721 aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc
     9781 ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc
     9841 ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt
     9901 gtcagcttgt gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa
     9961 tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat attttattat
    10021 tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg
    10081 agtttctaac attttatgtt ataaaacgta acatgcataa taagataaaa aagtggcgaa
    10141 agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt
    10201 attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat
    10261 acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg
    10321 attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg
    10381 actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca
    10441 ctaattattg cccataacaa aacattggct ggtcagctgt acggggaatt caaggaattt
    10501 ttccctaaca atgctgttga atattttgtt tcttattatg attattatca gcctgaagct
    10561 tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac
    10621 aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca
    10681 tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg
    10741 attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa
    10801 ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg
    10861 gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag
    10921 gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac
    10981 gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta
    11041 gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt
    11101 ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg
    11161 ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa
    11221 ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg
    11281 cacgtaacca tgcctcaagt cagaggaatg tataatggtg accgagcgag aaaacagatg
    11341 ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa
    11401 gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa
    11461 catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca
    11521 gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa
    11581 cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac
    11641 ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa
    11701 acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt
    11761 ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg
    11821 gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga
    11881 gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg
    11941 cgtctagcga tggatgaaac atcaagacgc cgtacgattc agcagaagta caatgaagaa
    12001 catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa
    12061 gaatcggaag atgatacaaa agaagcggta caagtctcgt atgaagaaat gacgaaagaa
    12121 gaaaaagata cgttattgat gaaattagaa aaagaaatga aaaatgcggc gaaagcattg
    12181 gattttgaaa cagccgctaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag
    12241 gaggacaaca tggctaacga taaaattacg attcacggtg cacgtgccca taacttgaaa
    12301 aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct
    12361 ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag
    12421 agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc
    12481 atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg
    12541 tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta
    12601 ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg
    12661 gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg
    12721 gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc
    12781 cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac
    12841 aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct
    12901 cgtctatttg attctttaga ggcggctctg cgattggcag acggctatgc tctggttgac
    12961 gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt
    13021 actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca
    13081 gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga
    13141 acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat
    13201 cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa
    13261 gaattaccgg aagagcatca ggatatcatc ttgaatggga cagatgatct tttccatttc
    13321 cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg
    13381 aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt
    13441 ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct
    13501 ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa
    13561 gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg
    13621 attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta
    13681 actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct
    13741 acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga
    13801 ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc
    13861 aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatctgatt
    13921 gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa
    13981 gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc
    14041 cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca
    14101 gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca
    14161 ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg
    14221 caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa
    14281 agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat
    14341 ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag
    14401 gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt
    14461 gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat
    14521 gttccttgtg aagtatgtca tggcaaacga tataactctg aaacattaga agtgcattac
    14581 aaaggaaaaa gcattgctga tattttggaa atgacagtag aagatgctgt agaattcttc
    14641 aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat
    14701 gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt
    14761 gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg
    14821 acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat
    14881 gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat
    14941 atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca
    15001 ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg
    15061 gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt
    15121 gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt
    15181 atagacaaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag
    15241 tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa
    15301 acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat
    15361 ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc
    15421 atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa
    15481 aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtgttta tcgagacgat
    15541 gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg
    15601 tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat
    15661 ctgtccatat attgattatt ctttatttga aagactgatc aataaagtaa gcttttatga
    15721 agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt
    15781 agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc
    15841 aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga
    15901 gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga
    15961 aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta
    16021 tgtagatgaa gatcatcaag ttcggatgat gtgtcttggt catccactgt tagaagatcc
    16081 tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta
    16141 tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat
    16201 gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg
    16261 tagcagtttc tttgtgacat tgaacggctt gaatctggct cgtttcgtca cagaagatcg
    16321 agtgtttaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag
    16381 attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa
    16441 aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtgt tttatgaaaa
    16501 agaccggtca atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag
    16561 attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt
    16621 aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa
    16681 agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga
    16741 tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga
    16801 tattgagaaa caaaatttat tgcagccgaa cttcattgtg ttgacttgta acaccgcaca
    16861 ctactttttt gaagaattac aagcggcaac ggatattcct atccttcata tgccaagaga
    16921 agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga
    16981 aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggat tcgaaacagt
    17041 gattcctgat actgttcttc aagaaaaaat caattatctg atctatcatg agatcaagga
    17101 atcggattat ttgaatcaag agctatatta cgaaatctta gaagaagcgg tagagcgcct
    17161 aaactgtgaa aaagttatct taggttgcac agaattgtca ttgatgcatg aatttgctga
    17221 agataaccat tatccagtga tcgatgccca atctatttta gcagatcgga cgattgaacg
    17281 agcgctagct gaaagaagca aggcattgga tacagcatcg gaaaagtaaa aaaattggat
    17341 ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac
    17401 tggtaatgat acgcccggaa tgatataatt ttactatgca caatgtgaga ggagttcgta
    17461 attatgacag ataatttgca attagtcatc ataacaggta tgagcggtgc agggaaaaca
    17521 gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga
    17581 ttgattccaa aattctggga attaatcaaa gaatcaggca aagtcacaaa aatggcttta
    17641 ggagtggatt tacgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc
    17701 gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta
    17761 gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact
    17821 gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc
    17881 attgatacga cgacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa
    17941 tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg
    18001 ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg
    18061 gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat
    18121 gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac
    18181 attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt
    18241 tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg
    18301 acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct
    18361 accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc
    18421 tgaaaagttt gcgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg
    18481 ggggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa
    18541 atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt
    18601 tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag
    18661 tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg
    18721 tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtctttaaag
    18781 acggaacgac tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc attgaccgtg
    18841 tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag
    18901 caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc
    18961 caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct
    19021 atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata
    19081 tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg
    19141 aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg
    19201 cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttgg
    19261 aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt
    19321 tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt
    19381 catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga
    19441 aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt
    19501 ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga
    19561 aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga
    19621 acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata
    19681 tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa
    19741 aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa
    19801 cgagccgtta tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg
    19861 atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt
    19921 cttatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact
    19981 caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc
    20041 tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg
    20101 aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg
    20161 aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga
    20221 ttccctcagg tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat
    20281 ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca
    20341 ggagatgcga atgtatcagt tttttttaaa agttatactt gcttttcttc cgtcaaacta
    20401 taattccatc tttactggag taacataatt tggaaaggct tctattgttt ttaatacaat
    20461 cgtataaaaa gcggggagac aactgaatga agattttagt tttgtacata aatttttgct
    20521 taatatagaa gtcctgatat agataagatc aagtatttat ctataactgg cctgattaat
    20581 taaaaaactg gaaggaactg ctcgatcatc cttgagaact gctcttctta cttctggtca
    20641 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    20701 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    20761 taatcataag ttcataaata gtaattgaga tgtatggttg gaatacgtta tacctgaact
    20821 tgaactacca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    20881 tgcgtttttt tacaactttt actatcatta tgattgcagt actttttatt tttctagata
    20941 tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca ggtctcataa
    21001 catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt tggagaactc
    21061 cagcaccacc attgccagag attacatatg gagaatttcc ttttagactt gagtatgaat
    21121 tgaatgaaga attacatgtg atagaagaca cgttaatagt tgaatttgat ggatttggaa
    21181 tgaacgaagg catagggaga tatcgtagat ggacaagtcg tttagccagc ggagaagatt
    21241 tagtgttgct gttagaagtt agtgataata agcaaatatt ttattttcca ggacctgcta
    21301 attattacat gggagatagg ttgaatggtt ataatcatac ttttcctagt gcttctttca
    21361 ttgaaaggga aagaggcatt actagaaggg atattttaca tgacaaagaa ttattagaac
    21421 aatttggtcc gcttgatcaa aatacaataa atgaagaaga attgttaaat caatataata
    21481 taaggctagt gaattgggaa atatctgaac cgattgttaa caattttggt gattagaaaa
    21541 caattacgat aaatagaaac aagccaaatc atcagcaagt aaaagctgat gatttggcct
    21601 ttcttatacg agtagtagga attcatagcg aatattatta cgatcagtag atattttgat
    21661 attaaaggag tattacgaac tagtatgtga gaatgtagag gtgatttgcg aataatgaga
    21721 aatttatcaa atagaaacaa aatattaatc attattgttg ttatagctgt gtttcatctg
    21781 ggaacgaatg cggttttaag tagaataata ttgggtccta agccaccaag acctgcgatt
    21841 acccgagggg agtttgattt tagattagaa tatgaagtag atggggaaag gattgtgata
    21901 gaagatacaa ttgttgcatt atttgatgga ttttctgcag atgcaggtag catggcatgg
    21961 tatcgcacgt ggcgtttgca tttggcgagt gacaagaggg cacaacaatt attgttacat
    22021 gagttagatg atggtcgaaa aatatattat cattttggag gagctggatt ttatatgggg
    22081 aagttagtag cagtgtaact gtaacaagga aagatttgcc aggtcttggc attcgaaact
    22141 ccatatttta tatcgagcgt gggagagata catctattgg gaacaggaaa gggttaactc
    22201 ttgaagagtt atataatgac tttggaatcc gtcttgttag ttgggaacac aatccaccaa
    22261 tagaaaatcg ctttgaataa tcgaaatcta ttaatccatt tcatcagcca gtgaatgttg
    22321 taatggaaca atttggttta gtgttcaaaa ccattgaaat actaggctgt gatcagcaaa
    22381 aatgtatctg aataaaaaag agagcaacta ttagctgaga ggagttaata gttgttagct
    22441 ttatacgaat caaagtggca aatacagatg cgtatgcatt tgtgtttgcc atcttgcgtt
    22501 tggtagacga gataggtata agctaaagaa acaacttgtt taaaataaac gacctagctc
    22561 aacaaatgac catctaatca actaacagca tacataaaag caactatttt tgaagtagaa
    22621 ttgaaatcgg gataacttaa aaagtgattg ggatactagt gagaatcacc gtacataaat
    22681 attactcgtt aagggaaatt gagaaatttt gtatgaaaat ttaagaatta gttagtatgc
    22741 gcaagtcaaa aatatgaata aattatttgg tggagataat ttgcttagct tgtcataaga
    22801 aatgagataa tctggcagat gcaagctttt ggacaccatt tattcggaat aa
//
LOCUS       DAYYNG010000031        22163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000031 DAYYNG010000000
VERSION     DAYYNG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.031"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1274
                     /gene="cls"
                     /locus_tag="ACHBFA_000974"
     CDS             <1..1274
                     /gene="cls"
                     /locus_tag="ACHBFA_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9394484.1"
                     /translation="SKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVKQ
                     LIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGEE
                     VRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRIN
                     YRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLMD
                     WNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREILI
                     QTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIET
                     YENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDSK
                     ISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            1611..2468
                     /locus_tag="ACHBFA_000975"
     CDS             1611..2468
                     /locus_tag="ACHBFA_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9394485.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            2583..3503
                     /gene="pstC"
                     /locus_tag="ACHBFA_000976"
     CDS             2583..3503
                     /gene="pstC"
                     /locus_tag="ACHBFA_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9394486.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            3503..4387
                     /gene="pstA"
                     /locus_tag="ACHBFA_000977"
     CDS             3503..4387
                     /gene="pstA"
                     /locus_tag="ACHBFA_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9394487.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            4402..5208
                     /gene="pstB"
                     /locus_tag="ACHBFA_000978"
     CDS             4402..5208
                     /gene="pstB"
                     /locus_tag="ACHBFA_000978"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9394488.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            5224..5982
                     /gene="pstB"
                     /locus_tag="ACHBFA_000979"
     CDS             5224..5982
                     /gene="pstB"
                     /locus_tag="ACHBFA_000979"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9394489.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            5995..6672
                     /gene="phoU"
                     /locus_tag="ACHBFA_000980"
     CDS             5995..6672
                     /gene="phoU"
                     /locus_tag="ACHBFA_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9394490.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            6804..7925
                     /locus_tag="ACHBFA_000981"
     CDS             6804..7925
                     /locus_tag="ACHBFA_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9394491.1"
                     /translation="MQAFEHRLFGKGFVTKHVTNPFHHFIERIKNAKKISIITIFFIC
                     VLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLITGKLMNSDQKGESIYLSVPYQKNQI
                     ETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            7922..8689
                     /locus_tag="ACHBFA_000982"
     CDS             7922..8689
                     /locus_tag="ACHBFA_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9394492.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            8789..10357
                     /gene="liaX"
                     /locus_tag="ACHBFA_000983"
     CDS             8789..10357
                     /gene="liaX"
                     /locus_tag="ACHBFA_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM9394493.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            10654..10974
                     /locus_tag="ACHBFA_000984"
     CDS             10654..10974
                     /locus_tag="ACHBFA_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9394494.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            10974..11324
                     /locus_tag="ACHBFA_000985"
     CDS             10974..11324
                     /locus_tag="ACHBFA_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM9394495.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            11491..12429
                     /gene="hprK"
                     /locus_tag="ACHBFA_000986"
     CDS             11491..12429
                     /gene="hprK"
                     /locus_tag="ACHBFA_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9394496.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            12444..13274
                     /gene="lgt"
                     /locus_tag="ACHBFA_000987"
     CDS             12444..13274
                     /gene="lgt"
                     /locus_tag="ACHBFA_000987"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9394497.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            13318..14343
                     /locus_tag="ACHBFA_000988"
     CDS             13318..14343
                     /locus_tag="ACHBFA_000988"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9394498.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            14358..15296
                     /gene="galU"
                     /locus_tag="ACHBFA_000989"
     CDS             14358..15296
                     /gene="galU"
                     /locus_tag="ACHBFA_000989"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9394499.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            15421..15861
                     /locus_tag="ACHBFA_000990"
     CDS             15421..15861
                     /locus_tag="ACHBFA_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM9394500.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            15864..16466
                     /locus_tag="ACHBFA_000991"
     CDS             15864..16466
                     /locus_tag="ACHBFA_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9394501.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(16529..17632)
                     /locus_tag="ACHBFA_000992"
     CDS             complement(16529..17632)
                     /locus_tag="ACHBFA_000992"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM9394502.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            17842..18843
                     /gene="ccpA"
                     /locus_tag="ACHBFA_000993"
     CDS             17842..18843
                     /gene="ccpA"
                     /locus_tag="ACHBFA_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9394503.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            19198..19593
                     /locus_tag="ACHBFA_000994"
     CDS             19198..19593
                     /locus_tag="ACHBFA_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394504.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            19593..19844
                     /locus_tag="ACHBFA_000995"
     CDS             19593..19844
                     /locus_tag="ACHBFA_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394505.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            19887..20282
                     /locus_tag="ACHBFA_000996"
     CDS             19887..20282
                     /locus_tag="ACHBFA_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394506.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            20279..21535
                     /locus_tag="ACHBFA_000997"
     CDS             20279..21535
                     /locus_tag="ACHBFA_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFM9394507.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            21547..21870
                     /locus_tag="ACHBFA_000998"
     CDS             21547..21870
                     /locus_tag="ACHBFA_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394508.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            21863..22129
                     /locus_tag="ACHBFA_000999"
     CDS             21863..22129
                     /locus_tag="ACHBFA_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394509.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         7435 a         3795 c         4750 g         6183 t
ORIGIN      
        1 tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag aacctggaaa
       61 ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct actggacaag
      121 tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc tatacgacta
      181 aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg attgaagata
      241 tcaagcaggc agaagaccat atccacttcc agtattatat ttatcgcagt gatgcattag
      301 gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc
      361 ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat ttgaaaaaag
      421 caggcggaga agtcgcattt ttctttcctc tatttgttcc gtatattaat cctcggatca
      481 attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat acaggtgggt
      541 tcaatgttgg aaatgaatat ctaggattgg taaagaaatt tggttattgg cgagacaatc
      601 atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg atggactgga
      661 attctcagca tacaaaagaa gtaggctatt ctcccaaatt tttcccgtcc attcacacta
      721 ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat gaacaaatca
      781 aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc caaacgcctt
      841 attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt tcaggcgtaa
      901 aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg gcaacgtatt
      961 ctttcgctgc agagctgatc gagtatggag caaggatcga aacatatgag aatggattta
     1021 ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca gcaaatatcg
     1081 atgttcgttc atttcgcttg gactttgagg tcaatacact ggtttatgat gaacgaatgg
     1141 cttcacgtgt aagaaaagct ttttttgaag actcgaaaat ttctacgcat ctcacaaaag
     1201 aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca agattgattt
     1261 cgccgctcct ttaaaacata aaaaggacat gagaaccatc aacagatgga gatctcgtgt
     1321 ccttttctaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg tctcgcatga
     1381 gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata agctaatata
     1441 tgctattaaa agagacacat ttcaaagcca gtatagcata gaaactttta atgagatagt
     1501 tttttttaca attatgagaa tttattttgt tatttaatga ttttgattcg ttttcatcaa
     1561 gtaatgaaaa tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac
     1621 ttaaattatt cttaccaata ataggattag ccattctttt atcaggctgt tcaaagtgga
     1681 ttgatcgagg agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa
     1741 cagttgctga aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg
     1801 gaagcggaac tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt
     1861 tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag
     1921 ttgttgggat cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa
     1981 acctgaaaaa actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc
     2041 agccagtagt tatattgaac cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat
     2101 gggtattgga cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc
     2161 gccagatcgt tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg
     2221 atgatgtaga tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga
     2281 acaaatggac gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga
     2341 caaaagaatt tttagactat atcttatctg atgaagtaca agataacatc gttggagaac
     2401 taggttatat ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta
     2461 aagagtaatc tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc
     2521 aaattgatgt tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat
     2581 ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc
     2641 gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt
     2701 tttattttgt agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg
     2761 attttctgtt tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg
     2821 taggggcatt gcctatgatc acaggttcgt ttattgtgac ctttttatct gctatcgttg
     2881 ccacgccatt tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa
     2941 cgaagatctt acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt
     3001 ttatcggttt atcagttatc gtgccttttg tcagaagtat ttttggcggg acagggtttg
     3061 gtatcttagc tggtacattt gtgttgttcg ttatgatctt gccgactgtc acaacgatga
     3121 ctgttgatgc attaaaggca gtaccacggc actatcggga agcttcatta gcgcttggtg
     3181 ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa
     3241 cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca
     3301 tcggtaatgc agcattgatg ccaacaggac ttactactcc tgcttctaca ttgacgtcca
     3361 tcttaacaat gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt
     3421 cattagcgct gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg
     3481 ggaagaaagg agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt
     3541 tttatatata gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt
     3601 agttcgggga ataccgcaca tttcatggtc cttcttgacc gaaccttcca aagcctacca
     3661 agaaggtggt ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat
     3721 gatcatcagt tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa
     3781 aaactggcta acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt
     3841 cgttgtcggt ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat
     3901 tatttctggt gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga
     3961 ggagtcttta caagcggtcc attacacaca acgagaagca ggattggcct taggtttgtc
     4021 acgctgggaa acagtgatga aagtggtggt tccagaagcg acaccaggga ttttgactgg
     4081 tgtgatccta agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg
     4141 acaaagtgca cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat
     4201 cagcattttc cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg
     4261 cacgatgcct gatggtgttt ctgtttcggc tggcgcttct gccgtgttga tcattgctgt
     4321 tttactgttc aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc
     4381 agcctaagga gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc
     4441 atgccaaaag agaaagaagt agcactatat acggatgata tacatgtctg gtatggcgac
     4501 aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt
     4561 ggtccttccg gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg
     4621 atcgctaata cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct
     4681 caagtggatg tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg
     4741 ttcagcaagt cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac
     4801 aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa
     4861 gtaaaagaca gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta
     4921 tgcattgctc gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt
     4981 gctttggacc ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac
     5041 tatacgatca tcatcgtgac gcataacatg caacaagcag ctcgtatcag cgactatact
     5101 gcctttttct atctaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga
     5161 ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc
     5221 aacatgacaa aagaaattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa
     5281 gcactaaaag ggatcgatct aagtttcaat caaggggaaa tcactgcgat gatcggtcct
     5341 tctggttgcg gaaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg
     5401 aatgtaacga tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca
     5461 gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct
     5521 ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa
     5581 gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa
     5641 gacaaattgc ataaaagtgc tttatcttta tccggcgggc agcagcagcg tgtatgtatc
     5701 gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt
     5761 gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg
     5821 atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc
     5881 tttttagatg gtcacttgat cgaatacaac gatacgaaga agatattcat gaatccagaa
     5941 aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta
     6001 cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg
     6061 atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta
     6121 gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag
     6181 aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt
     6241 acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct
     6301 aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc
     6361 gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca
     6421 gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat
     6481 cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga
     6541 acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc
     6601 tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat
     6661 gagattttct aaactgaagc tgtgattatg ccgtttgttt atgaaaagag gttgtgaaaa
     6721 agctgttcta catcaagtaa taagaacagt caaacaaaaa atagctcctc atatttttcg
     6781 tttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc gggaaagggt
     6841 ttgtgacaaa acatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
     6901 aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg gttattcagc
     6961 atcaagccgc tttatatgaa gtacctgtca tcgagataga aaaagtacga ataaaagagg
     7021 atcatcaatt gattacagga aaactgatga actcggatca aaaaggagaa tcgatttatt
     7081 tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg agacaaatgt
     7141 atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat gatggctggc
     7201 ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggcgga aaacagggtc
     7261 tatttgcttt aggaagtctc atcatcaata tcgttttact tttacttttg ttgctcatct
     7321 ttactaaaac agaagggatt tctctattaa ataaatctat cctgttcgtc gttataggga
     7381 tgattattag catattggct ttagatggat ggaatagaag cagtataatc aaaatttcag
     7441 ctgcagttac gagtgttttt ttagcatttt ttatttgtct tttgacaatg aatcattgga
     7501 aagaccaagg actgcgctat gaggaactga attatctgac acgaccgtat cgttctgttt
     7561 ttctttctag cgtactgatt ggaacagctg gaggaacact cgataccgtt atcacagtca
     7621 ttgcgacatt agaagaattg actaagcgac aaccagaaat aacagcgaaa cagttatggc
     7681 tttcaggaag gcaagtgggt cgagatgtga ttggctcgat gaccaatatc cttttgttcg
     7741 tctatatcag tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa tggtctttca
     7801 aagaaacgat agaacagcat ttatcattgg aatttttacg cgtcattgct ggcagcttag
     7861 gaatagtatt atcgatcccc atctcggtcc tcttttttct gactgtaagg aggctgaaac
     7921 gatgaatgta cttattatac ttgttgggat tttagcaatc agcgtgctac ttattggcgg
     7981 tacgcaaggt atgtatattc ttttaggctt attcatcaat ttagggatct ttttcctgtt
     8041 gttattcggt tatcatcaga agtggccgat actggcattg tcggtcatag gatttttatt
     8101 gattgctgtg gttatcttat tttttatcaa cggttataat ctaaaaatgc gtgcagcttt
     8161 tgtgagtata ctgatctttt tattttgttt cctactattg cttcctatca ctgatttttt
     8221 agcgatccaa ggttttacat cggtagaact agaagaatta tctggcttag ataagacttt
     8281 agcgattgat tttagattac ttacgagatc gttgcttctg attagtctta gtggtgctgt
     8341 tttagatgcg agtgtagcaa tttctagtgg gacattcgaa gtatatcagg caaatccgca
     8401 attatctttt taccaattgc gccattctag tttcgctatt gctaaaaaaa tattggcgtc
     8461 aacagtaatg acacttcttt ttgcattcat gggaagttct ttagcgttga tcctctggtt
     8521 tattgattta gacattcctt ttcagcaagt gatcaatgaa aaatcatttg ttctagaata
     8581 tacgatggct attcttacca cattgtccgc actaattgtt cttcctttaa cctgcgtgac
     8641 tacagcatat ctctttacaa aaaagaaaga acaactcaag atggagtaaa agttacaact
     8701 taagtcctat gacattttta ctgaatcagt gcataatatg gatgtaagga aaagcttgca
     8761 aacaattttg cttaaggagg caatttccat gaaagaaaga gaacgtattt tagatttagt
     8821 gaaaaaagga atcctttcga ctgaagaagg cttagattta ttagaaagca tggcaactga
     8881 aaaagacgaa aagcaaatca aaaaagaagc agacaaggta actgctagtc ataaagaaaa
     8941 agataaggca agtcaattga tcgataaact tgaaaacggc gaagaagaaa tttctgagcc
     9001 agttgatcca aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga tcttagatga
     9061 actagcaact gaagcgaata aaacttcggc gcgtctagat gaagtaaatg ctcaattcgc
     9121 tgacaataag gcagcacgca atgagaaaca ggaagcattg atgcagctga atacaaaaga
     9181 agaattaggc gagttgacag aagaagaatt agcacagcgt caggcgttag aggcagaaat
     9241 caaagagtta gaagctagtg gcgatacact gttagaagaa caattgaaat tagaagcaga
     9301 attaaaagat atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta ctgataagtt
     9361 tgaattgcca gatgattgga aagatcaagc aacagataca ctgaatcaag taggcgaaaa
     9421 aatgtcagaa gctggatcac agttaggcaa attcttgaag aaaacattcc aaactgtttc
     9481 agaaacagtg aatgacaaca tggaatggaa agatgtcagc ttacgtgtcc ctgggattgc
     9541 gacaacgaaa tttgagcatg aattttacta tgaagctcct gcagcaagta tccttgatat
     9601 caaggcagca aacggaaacg tgacgttaaa aacgtgggac agcaacgatg tgaaagttga
     9661 agctaagatc aaactttacg gaaaaatggg tgctgaacca tttgaagcat tctctgaacg
     9721 gagccagatt gaggttaatg aagatcatat ctccttccaa attccaaata aacgtgtgcg
     9781 tgcagacctt gttttctact tacctaaacg agtatacgac cacgcagcta ttaaacttct
     9841 aaatggcaac atcatgattg aaacattgga agctaaagat atttatacaa aatctacgaa
     9901 tggtaacatc atcgtggacc aattaactgc aacgatgctg gaagtagaag gtgtaaacgg
     9961 caatatcgat attcgaaacg ggaacatctt agattctatt atcgaaacag taaatggtac
    10021 agtgacattt ggtgcgacag cagaaaatct ttctgtgtct ttagtaaatg gtgatgtacg
    10081 tcttacgatt aaagaagata atttgaaaaa agtagaagca agttcagtga acggaaatgt
    10141 caaagtcgct ttaccagatg gcatcggttt ggaaggacat gctaaaacaa gtcttggaag
    10201 tatcaatagc cgtctgtcca attacgaagt agttcgtgag aaaaaagaac ggacaaacca
    10261 aatgttgcaa ttccgtcgtg tttcagatga tgagatcgcg caagtgcaac tttcaacgac
    10321 aacaggaagt atctatttaa aagatacaga taaataaaga ggttgtgaaa gagatgttca
    10381 gccttgagca ataagaagcg attttgtaaa atagcttccc ctattttaca aaattgtgcc
    10441 ttattgccga tagtctatct cttgaacacc gtttaccaag agattatgaa cctgccaatt
    10501 atcgccgagt cacaaggaat aatttcagaa aaagctcgtc acgttttatg aatatggtaa
    10561 tttgtgaccg agaattttgc agaaagacac cgattaagaa gaaggttgtc acaacgattt
    10621 tcttcattta aaatagtaga acagaggtga gacatgatga aaaaaaagtt gacaaaatct
    10681 aataaaaatg ttgttttgac agggacttta gcaggaatag gagaatacat cgggattgat
    10741 ccgactgtta tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg aagtccaatt
    10801 attttataca ttttattagc tttgattatc ccatctgcgc gttctgcacg aacaaactat
    10861 ggacataaca atgcatatta tgaaaaaaac aattatcgtg aacaaactcg tgaacaaaca
    10921 aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat ggagtgactt ttaatgtcgt
    10981 actttcaacg tattttagtt aatacgttaa cattcatatc attggctgtt ctttttcctg
    11041 cgatggtcca tgtgagcagt atttggacgg ccgttcttgc cagcttcgta ttgtctgtgt
    11101 tgaatatgtt tgtcaaaccg attttgacga tactgtccct gccctttaca ttagtcacat
    11161 tgggattgtt tacgtttgtt atcaatgcag cgattcttaa aatgacctcc ttttttgtgg
    11221 gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt gttagtagct atcttgatgt
    11281 cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa ataaaagaaa gctgtgccga
    11341 actatcggcg cagttttttt atttagaatc aggaatgcgt tttctattac attggtaatt
    11401 aaataaaaac gtttttttta attggtagta cttttggcta gacaatgata aaatgataga
    11461 gaacactcta atagaaatga ggtagcatga atgtcagaag ttgtaaaagt tagccaatta
    11521 gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga aaagcttaga ccgtgtcatc
    11581 gtaactgggg acatctcacg cccaggacta gagttgactg gttattttaa ttattattcc
    11641 cataatcgtt tacagctatt tggcagtaaa gaaatcagtt ttgcggaacg gatgatccca
    11701 gaagagcgtt tgatcatcat gagaagatta tgtagtgaag atatgcccgc atttattatt
    11761 tcaagaggat taccagctcc agaagaaatg atccaggcag caaatgaaaa tgggatagct
    11821 gtactgcgtt ctcccatctc aacttctcgt ttattaggag aattatccag ttatttagat
    11881 ggtcgccttg cccctcgaac aagtgtccac ggtgttttag tggatgttta cggattaggt
    11941 gtactgattc aaggtgacag tgggatagga aaaagtgaaa cagcgttgga gttgatcaaa
    12001 cgcggccatc gattgatcgc agacgatcga gtagatgttt atcagcaaga tgaattaacg
    12061 gtgatcggag aaccgcctaa aatcctggaa catctgattg aaatccgagg aatcgggatc
    12121 attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg gctctatgca agtccagttg
    12181 gctgtttatc tggaagcatg ggcaaaagat aagaaatacg accgtctggg ctctgaagac
    12241 acgaccgttg aaatcgcgga agtgggcatt ccacaaataa aaatccctgt caaaacagga
    12301 cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt tccgtgcacg tacaatgggc
    12361 tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct tgattgaaga aaactcaggc
    12421 aacgactagc aaggagtgac aatatgttag cacaaatcaa tcgagttgct tttgagcttg
    12481 tagggattcc tatctactgg tatgcattga tcatcgtatc aggtatcatt atcgctatgt
    12541 ggcttagcag cagagaggct gttcgggtag gattaaaagc agatgatgtg acagatttta
    12601 tgttgtgggg attgcctatc tccattattg gcgctcgctt atattatgtt ttatttgatc
    12661 ttccccagta tatagctgat ccaatccaaa tattcaatat ccgttcagga ggcttagcta
    12721 tttatggcgg actgattgca ggcgcgatca cgctgtattt ttttactaaa tatcatttta
    12781 tttctttgtg gactttttta gatatcgctg caccaagtgt attattagca caggcaatcg
    12841 gtcgttgggg aaatttcatg aaccacgaag catatggacc agacacgacg cgaagcttct
    12901 tagaaagtct gcatttaccg caattcatca ttgataatat gtatatcgaa gggatctatc
    12961 gtcaaccaac atttctttat gaatcacttt ggagtttgat tggatttatt ttgcttttgt
    13021 ttttacgaaa gaaaccacac cttttgaaaa aaggcgaagt ccttttatgt tatctaatgt
    13081 ggtatagttt cgggcgtttc tttattgaag gattacgtac agacagtctt tactttttag
    13141 gtgtgatccg tatttctcaa ttattatctg ctgtgttatt tgtcggtaca ttgattctat
    13201 ttatttggcg cagaaaaaaa ggaaatttac cggattatga ccgctcatta ggaaaaaatc
    13261 aacaaattat ctgattttta gagcgttaaa aatgaaaaaa atttcaggag tggtaaaatg
    13321 aattatagag tagcggtttt aggaccaggc tcttggggta ctgctttggc acaagtcctc
    13381 gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg cttcacaagt agaagaaatc
    13441 aacacccaac acacgaatca acgttattta ccagatcatt atctacccga ttccatccaa
    13501 gcattcacca atatggaaga tgcagtaaaa gacgcagatg cggtgctatt cgtcattcca
    13561 acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta aattgaatac aaagccagtg
    13621 atcatccatg caagtaaagg attagaacag ggaacacata aaaggatctc tgacatcttg
    13681 ttagaggaaa tcccagcaga aaaaagaaaa gctgttgttg ttctttctgg accaagccat
    13741 gcagaagaag tagcaaaaca tgacatcact acgatcacag ctgcaagtac agatgcaaaa
    13801 gcagcaaaat atgtacaaga gctgttcatg aataattatt ttcggatcta taccaatcct
    13861 gatgtgatcg gtgtagagat gggggcagct ttgaaaaata ttattgctat tggtgctggc
    13921 gctattcacg gcttagggtt tggcgataat gcaaaggcag caatcatgac gcgcggatta
    13981 gctgagatca gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt tatcggatta
    14041 agcggaatag gcgatctagt cgttacttgc acaagtgttc attctaggaa ctggcgcgca
    14101 ggcaacttgt taggacaagg gcagccccta gaagaggtgc tagccaatat gggaatggtc
    14161 gttgaaggtg tgaatactac ccaagcagcg atggaactag cccaaatgat ggaaatcgaa
    14221 atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat caaaacagcc
    14281 gcaaaagaaa tcatgttacg tgatggaaaa aaagaaaatg aattctcatt aacaattttt
    14341 tgaaggagcg aatgtatatg aaagttaaaa aagcagttat tccagcagca ggattaggaa
    14401 caagattttt accagcaaca aaagctatgg caaaggaaat gttgccaatt gttgataaac
    14461 caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac atcttgatcg
    14521 ttacaggaaa agctaaacga ccaatcgaag atcactttga ttcaaatatt gaattagaaa
    14581 caaacctaag tgaaaaagga aagaccgaat tattgaaatt agtagaagaa acgacagatg
    14641 tcaacttgca tttcatccgt caatctcatc caaagggttt aggacatgcc gttcttcaag
    14701 caagagcctt tattggaaat gaaccatttg tcgttatgct aggcgatgat ataatggaag
    14761 acgaagtacc attgacacag caattgatcg atgactatga gcgtacacac gcttctacga
    14821 ttgccgttat gcaagttcct cacgatgaaa cttcaaaata cggaatcatc aacccagggg
    14881 aagtactgga agatgggtta tacaatgtca aaaactttgt tgaaaaacca gatccaagca
    14941 aagcaccaag tgatttagct attattgggc gttacttatt gactccagaa atctttgatg
    15001 tattatcaga acaacaacca ggtgctggaa atgagatcca actaactgac gcaatcgata
    15061 cgttgaataa aacacaacgc gtatttgccc gtaaattcac aggtaaacgt tatgacgttg
    15121 gtgacaaatt tggttttatg aaaacaagta ttgaatatgg tctgacacat ccagaagtag
    15181 gggcaccttt gcgtgaatac atcatgtctt taggtaaaac attagaagca gaaggaagca
    15241 agatcaatca taatccaaaa ggaataacag aaatcaaaga agaaaagaaa aaataatctt
    15301 tttttctaac gagcttcagc tgctaattga ataaaaagta gttgaagctc gtttttattt
    15361 gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga gacaagaagg gagtttgatc
    15421 atgtcaggag gagaaattgc aggattgatc gcagcaatcg cctttgcgat acttgtcgtt
    15481 ttcatcgttc gcgtattgtt gcaagtttcc aaaactttag aaaaagtaga ccagactgtc
    15541 gcagaagcaa atacgacaat tgagatcgtc acacaagacg tagatctttt atctagacaa
    15601 gtagaaggct tgctagtcaa aagtaatgag ctgctcttgg atatcaatgg aaaagtcgca
    15661 acgatcgatc cattgttcac agcagttgct gatttaagtg aaagtgtatc ggaacttaat
    15721 cattctagta aaaacatcgt gacgaaagtc ggcggaatcg gaaaaacgac agctaaagcg
    15781 actgttgccg gaaaagtcgg tggaacagcg atgaaatttt ttaaaaacaa aaaacatact
    15841 gaaacgacag gaggaaaata agaatggcta aaaaaggtgg atttttatta ggtgcagtag
    15901 ttggaggtac agccgcagca atcgccgcat tattgttagc acccaaatct ggtaaagaac
    15961 ttcgggatga attggctgct caagcagatg atttgatgga catggcttca gattacacag
    16021 atatggcaaa agaaaaatca tctgagttaa caggtattgc aaaagaaaaa gcgcaaggtc
    16081 tttctcaaca agcaggggat ctagcaggca atgttaagca gaaggtaagt gaatcagcag
    16141 aggaagcagg aagtgaatca gatgatatct tgttaaacct caaagaacaa tcagctgatt
    16201 tatctaatcg tttcaaaaaa acagcagaag atctgaaaaa gccagcagac gaattaggta
    16261 gtattgctaa agatacttca gaagatatca tgatcgatgt aaaagatgct gcagataaag
    16321 caggagagac ggtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gacacaaaag
    16381 aaatgttgaa aatggcaaaa gaagatgcaa aagaagctaa aaaagaaatg aaagatacaa
    16441 tgacagattc gactaccaat cactaagatt actcatttct agaataaaaa aggtgagagc
    16501 ggaccagctc tcaccttttt tattttcatt actctaaaac aagtaattct tttggtgtag
    16561 tcgtgaatgg cacacaacca tcacttgtca catacacaca atcttcgata cgtaccccta
    16621 ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct tcaatcacta
    16681 agtcgtttcc ttctacaaga gaagggaatt cgtggacggt tgttccgatt ccgtgaccta
    16741 aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat
    16801 caagttcacc tgctgtcaca cctgggcgaa ctgcctcttg tgcggcaagc tgtgcttcaa
    16861 gagtgatgtt atagattttt tcttggaact cagttggttt ttgataagca actgtacgag
    16921 tagcatcact gcaatagcca ttccaaataa cgccaagatc gaataacact agttcatttg
    16981 gtgaaacctt cgtgcttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga
    17041 ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt taattggtat tcgatttctg
    17101 cgataattgc ttgttcagtt acgccttctt ttactgtttt gaagccaatt tcaaaggcaa
    17161 catcggccca tttaccagct tcgatcagtt tgtctttttc ttctaccgtt ttgatcagtt
    17221 gcagacgctg gataatagaa gttagatcgg tgtaagaatc tgtacctgtc aagatatcag
    17281 agatagcatc gaagcgatcg acagtcagag cattcttttc aatacctaca ttaccaggtt
    17341 gcccatagcg tgcgcgaagc tcttttccga tgatctccca aggattttcg ctgtctaagt
    17401 agccacgtac atcgtacgcc cagctgctgt tttgagcatc ttcaacttcc agagcaggag
    17461 tgaaaaggaa aggatcttgt tccagaggga taaaaagagc tagtacacgc tcgtgaggat
    17521 cactttcata tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcta
    17581 cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac
    17641 ctactttcta aattaatttt gtattcaata attgtaccac tgattgatta aaagaccaac
    17701 gttcttaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt
    17761 gttgagatta tttgatatcc tattttagaa atgtaagacg tattttcaga aaacatataa
    17821 aatatgaata ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga
    17881 gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgtcaaa
    17941 ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaatgca
    18001 gtcgcacgtg gcttggcaag taagaaaaca acaacagttg gggtaatcat tcctgatgtc
    18061 agcaatatgt tctttgcctc tttagcacgt ggtatcgacg atgtggcaac aatgtacaaa
    18121 tacaatatca tcttagcaaa ctcagatggc aacgatcaaa aagaagtcaa tgtattgaat
    18181 aatttattgg ctaagcaagt ggacggcgtg atctttatgg gtcaccatat cacagacgaa
    18241 atccgtggag aattttcacg ttcaaaaacg cctgttgtac tagctggttc cattgatcca
    18301 gatgaacaag taggtagcgt aaacattgat tatgtgtcag caacaaaaga tgcagtaaat
    18361 aaattagcaa aaagcggcaa taaaaaaatc gcttttgtca gcggtgcgct gattgatcct
    18421 atcaatggtc aaaaccgtct aaaaggctat aaagaagcat taagcgaaaa caatctacca
    18481 tatagcgaag gattgatttt tgaggcacaa tacaacttta aagatggtct gtctctagtt
    18541 gaccggatcc ataatagcgg tgcaacagct gtctatgttt cagacgatga attagccatt
    18601 ggtatcttgg atggtttatt agaccgcggc gtaaaagtac cagaagaatt cgaaatcatc
    18661 acaagcaaca attctttact gacagaagta gctcgtcctc gtttgacaag tatcacacaa
    18721 ccattatacg atttaggagc ggtatccatg cgcctattga cgaaaatgat gaataaagaa
    18781 gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atcaactaaa
    18841 taataaaaga tcaatcattt aaaggttgtt ccaaaagtgg tcaacttctg gaacaacgtt
    18901 tatacgagaa agggggttat gacaaaattt tgtcataacc cctttttttt tgtgacttta
    18961 aaaaaagaga aaatggcgga aacaaacctt acgtccatcg tatacgacac aatctgtgcg
    19021 tagatgctac attttgttca ctactatcaa aattacttta aaatgaaaac gtgataaaaa
    19081 agaaagtaga aaagtagata agtaactcta aaaaatccaa aaaaatccag gctcgattgc
    19141 gtacttatct taaaggagaa acaatccatc atatataacc cgaaaggaga cttctatttg
    19201 cctaagaaaa gacgaaagac catagtcatc attttgacca ttcttactat aggaatcgga
    19261 gggaaatttt acatggacaa aagagaaatg caaagacaga aagatttact cgcagtagaa
    19321 aaacagtccg tcaaagttct gaaaaatacc tttgcggata tcaaggaagt gaagattgaa
    19381 aaatctgctc gaaatgaaat gacgggttca taccggatag ttattctaat gactaataaa
    19441 caagatcaat cgatttattt ttcttatagt ttttggaaag aaagaaatga gataggaagt
    19501 tacggaatag ttgatgaaaa aaagcaaaaa gaaggaaata cgctcaataa agttaaagta
    19561 acttactcta atggaaatga ggaaagtatt taatgattac tgacaaacaa tatagtgaat
    19621 taagtgatgc tgtttactgg ctagatccta aacatagaga ctatgtacct gaaatgcaag
    19681 aaaatttaag ttttaaaatt aacggcacta tgtataaaat cctaaaaatc aaaaactcct
    19741 tcgacggcat gcaggcgatg gctgtagcgc caatcgtcca tagcaaattg gagaaaaatt
    19801 tcaaaaataa aaaaattcct gcaaactttc gcgtacttaa ataatgaagg tgtcaatact
    19861 tacataaaaa gaaaggaggc acgttcttgt caaggaaaat aagaaaactc agcttagcca
    19921 ttttattaat cgtcatatta gggatcggag ggaaagtcta tatggacaaa agagaagcac
    19981 aaaggcagaa agatttactc gcagtagaaa aacagtcagt caaagttctg aaaaatacct
    20041 ttgcggatat cagggaagtg aaggtagaag aatttaaaaa aaaccctgtt accggaagtt
    20101 atggtgcact tgtgacaatg acaaataatg aagggaaatc tgtttatttt gattatagtt
    20161 tttggaagga gagaaatgaa cttggtggtt ttggcattga gaacagagcg gtacaaaaga
    20221 caggagtcac tataaaaaga gtaaaagtaa ttttttctaa cggacaggag gagttagtat
    20281 gacaataaac gataagcatt acaatgatat tagtgagaag gtttattggc tagatcccaa
    20341 atatccccgc tacaatgagg gatacaaaaa gaactctgtt aaagaatttg ctggtatgga
    20401 atttcaaatc ctacaaatca aagactcatt ggacggcatg caggcgatgt ctgtagcgcc
    20461 aatcaagaac ggtaaagtgg atctttctca agtagtgatt gcttttgcag gaacgaactt
    20521 aagtgattgg aaagatttgg aaacggatgc aagaagtatc atgaatctct acgggaatag
    20581 accaggagca agtttttctg aaaaagcaac aacaaacaag attttatccg ttagtggatt
    20641 gaaaacagcg gggcaaatca attcagcgat tgcatttgct aatgaagtaa aaaaggaata
    20701 tggagaagct gctatttcta ctaccggcca ttcattaggt ggattcttag cattatatgt
    20761 agcagcagag aaacaatgga aaaatgtcgg cttcaacggg ccggacccgt atgatctctt
    20821 atcaccagaa gcaaaaaaat gggtggaaga aaatcccggt atgctcacca attaccgaaa
    20881 tcacgccgat tttatcggta attttggtgg taatggcaca ggtgctgaag ttcggatcag
    20941 tatggaaaca gcattcttga atctgcttgc ttcccacagt ttaacctctt ggaagtttga
    21001 tgaaaatgga aagctgatta ttcctgaaaa tgaaaacaat gttaaagctc gaaaacaaca
    21061 gaaagaaaac caagcgaccc agttgttttc ctatcagttg caagaactga acaacatacg
    21121 taaaaaacta cgattaagtg gcggccggct gacagttaat gaacaaattt acttagatga
    21181 cacacaagct aggttagtag tggaaaagtt atcggcaaaa atgaaaagcg ctatggaaga
    21241 agttatccgc atcaatcaaa gaggaatttt tgagctagag aaaaaatggc aagaaggctt
    21301 gcggcaagca cgtgttgtag ccccttcgtt gactgaacag gaaatcatag aagcattagc
    21361 tagtgtgggc gccacaaaag aaaacttagt cgattctgcg aaagaagtct atctgctacg
    21421 aatcaataag gcaagacgta tgggggaggc ctttgataca ttggcgaagg agatccaaga
    21481 caaaatcaat gaaatcgtgg cacgagatcg agaattagca cagcaattca attaaaggag
    21541 aagtagatga aactagaaga attggaaaga gattatcgaa gtgaacgaag acaattagaa
    21601 gctcaagaag atgaattgat ccactttaag aatcaaggat tacgattgat ggagcaagag
    21661 gagcagcgac tgcatcatgt attaaagaac ttagctttag ataatgagcc tctgtttcaa
    21721 gcaagacgaa gttattcgaa ttatcaggag cattatcaac aagccctaca agtagaacga
    21781 cgaaaactct atcagcagca agatgaacta gaacgttcct atcgtcaaaa aatcagacaa
    21841 ataactatgg agaaatcatc ttatgaatga atttttaatg caattactaa aagaattaga
    21901 gaaaaatcta catcgatcaa atgtacgttc agttggtatg gggatatctc agcaaatttg
    21961 ggcagaaaaa cgattgacag atcaaaacta ccaaatgact gcagaagaag ctgctcaaga
    22021 attttctaac cgacatgtac acttgaaagg atcagtaaaa gggcaatttg cagaaaaact
    22081 aactgaatta ttccaaagac aagaaaagaa attaaaacag tttcgttagt aaagttatat
    22141 agaaaaattt tgtgaaaatg gtt
//
LOCUS       DAYYNG010000032        21934 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000032 DAYYNG010000000
VERSION     DAYYNG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.032"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1057)
                     /locus_tag="ACHBFA_001000"
     CDS             complement(89..1057)
                     /locus_tag="ACHBFA_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9394510.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1289..2122
                     /locus_tag="ACHBFA_001001"
     CDS             1289..2122
                     /locus_tag="ACHBFA_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM9394511.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2156..2995
                     /locus_tag="ACHBFA_001002"
     CDS             2156..2995
                     /locus_tag="ACHBFA_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9394512.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3021..3581
                     /locus_tag="ACHBFA_001003"
     CDS             3021..3581
                     /locus_tag="ACHBFA_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9394513.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3780..5501
                     /locus_tag="ACHBFA_001004"
     CDS             3780..5501
                     /locus_tag="ACHBFA_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9394514.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRTLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5666..6808
                     /locus_tag="ACHBFA_001005"
     CDS             5666..6808
                     /locus_tag="ACHBFA_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9394515.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6824..8035
                     /gene="thiI"
                     /locus_tag="ACHBFA_001006"
     CDS             6824..8035
                     /gene="thiI"
                     /locus_tag="ACHBFA_001006"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9394516.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8608..9255
                     /locus_tag="ACHBFA_001007"
     CDS             8608..9255
                     /locus_tag="ACHBFA_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9394517.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9384..9600
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9648..12293
                     /locus_tag="ACHBFA_001008"
     CDS             9648..12293
                     /locus_tag="ACHBFA_001008"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9394518.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12603..13916
                     /locus_tag="ACHBFA_001009"
     CDS             12603..13916
                     /locus_tag="ACHBFA_001009"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9394519.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13906..14559
                     /locus_tag="ACHBFA_001010"
     CDS             13906..14559
                     /locus_tag="ACHBFA_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9394520.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14614..15306
                     /gene="radC"
                     /locus_tag="ACHBFA_001011"
     CDS             14614..15306
                     /gene="radC"
                     /locus_tag="ACHBFA_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM9394521.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            15456..15656
                     /locus_tag="ACHBFA_001012"
     CDS             15456..15656
                     /locus_tag="ACHBFA_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9394522.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(15910..17589)
                     /locus_tag="ACHBFA_001013"
     CDS             complement(15910..17589)
                     /locus_tag="ACHBFA_001013"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9394523.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(17591..17803)
                     /locus_tag="ACHBFA_001014"
     CDS             complement(17591..17803)
                     /locus_tag="ACHBFA_001014"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9394524.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            18196..19926
                     /gene="efrA"
                     /locus_tag="ACHBFA_001015"
     CDS             18196..19926
                     /gene="efrA"
                     /locus_tag="ACHBFA_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM9394525.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            19923..21683
                     /gene="efrB"
                     /locus_tag="ACHBFA_001016"
     CDS             19923..21683
                     /gene="efrB"
                     /locus_tag="ACHBFA_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM9394526.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         7165 a         3813 c         4652 g         6304 t
ORIGIN      
        1 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
       61 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
      121 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
      181 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
      241 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
      301 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
      361 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
      421 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
      481 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
      541 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
      601 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
      661 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
      721 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
      781 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
      841 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
      901 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
      961 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     1021 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     1081 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     1141 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     1201 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     1261 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     1321 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     1381 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     1441 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     1501 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     1561 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     1621 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     1681 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     1741 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     1801 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     1861 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     1921 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     1981 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     2041 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     2101 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     2161 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     2221 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     2281 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     2341 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     2401 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     2461 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     2521 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     2581 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     2641 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     2701 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     2761 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     2821 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     2881 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     2941 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     3001 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     3061 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     3121 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     3181 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     3241 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     3301 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     3361 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     3421 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     3481 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     3541 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     3601 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     3661 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     3721 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     3781 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     3841 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     3901 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     3961 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     4021 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     4081 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     4141 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     4201 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     4261 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     4321 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     4381 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     4441 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
     4501 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
     4561 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
     4621 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
     4681 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
     4741 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
     4801 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
     4861 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
     4921 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
     4981 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
     5041 aagaaaagat cgatacattc gaattcaaat tgcgcacttt gaaacgtttt gtagaaaaac
     5101 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
     5161 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
     5221 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
     5281 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
     5341 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
     5401 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
     5461 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
     5521 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
     5581 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
     5641 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
     5701 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
     5761 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
     5821 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
     5881 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
     5941 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
     6001 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
     6061 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
     6121 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
     6181 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
     6241 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
     6301 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
     6361 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
     6421 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
     6481 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
     6541 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
     6601 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
     6661 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
     6721 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
     6781 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
     6841 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
     6901 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
     6961 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
     7021 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
     7081 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
     7141 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
     7201 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
     7261 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
     7321 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
     7381 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
     7441 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
     7501 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
     7561 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
     7621 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
     7681 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
     7741 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
     7801 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
     7861 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
     7921 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
     7981 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
     8041 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
     8101 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
     8161 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
     8221 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
     8281 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
     8341 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
     8401 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
     8461 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
     8521 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
     8581 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
     8641 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
     8701 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
     8761 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
     8821 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
     8881 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
     8941 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
     9001 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
     9061 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
     9121 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
     9181 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
     9241 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
     9301 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
     9361 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
     9421 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
     9481 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
     9541 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
     9601 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
     9661 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
     9721 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
     9781 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
     9841 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
     9901 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
     9961 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    10021 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    10081 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    10141 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    10201 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    10261 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    10321 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    10381 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    10441 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    10501 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    10561 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    10621 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    10681 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    10741 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    10801 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    10861 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    10921 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    10981 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    11041 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    11101 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    11161 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    11221 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    11281 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    11341 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    11401 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    11461 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    11521 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    11581 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    11641 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    11701 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    11761 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    11821 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    11881 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    11941 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    12001 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    12061 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    12121 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    12181 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    12241 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    12301 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    12361 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    12421 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    12481 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    12541 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    12601 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    12661 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    12721 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    12781 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    12841 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    12901 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    12961 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    13021 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    13081 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    13141 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    13201 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    13261 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    13321 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    13381 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    13441 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    13501 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    13561 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    13621 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    13681 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    13741 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    13801 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    13861 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    13921 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    13981 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    14041 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    14101 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    14161 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    14221 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    14281 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    14341 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    14401 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    14461 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    14521 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    14581 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    14641 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    14701 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    14761 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    14821 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    14881 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    14941 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    15001 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    15061 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    15121 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    15181 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    15241 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    15301 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    15361 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    15421 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    15481 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    15541 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    15601 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    15661 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    15721 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    15781 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    15841 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    15901 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    15961 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    16021 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    16081 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    16141 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    16201 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    16261 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    16321 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    16381 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    16441 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    16501 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    16561 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    16621 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    16681 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    16741 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    16801 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    16861 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    16921 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    16981 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    17041 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    17101 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    17161 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    17221 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    17281 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    17341 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    17401 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    17461 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    17521 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    17581 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    17641 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    17701 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    17761 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    17821 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    17881 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    17941 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    18001 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    18061 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    18121 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    18181 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    18241 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    18301 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    18361 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    18421 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    18481 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    18541 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    18601 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    18661 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    18721 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    18781 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    18841 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    18901 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    18961 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    19021 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    19081 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    19141 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    19201 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    19261 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    19321 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    19381 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    19441 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    19501 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    19561 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    19621 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    19681 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    19741 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    19801 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    19861 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    19921 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    19981 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    20041 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    20101 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    20161 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    20221 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    20281 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    20341 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    20401 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    20461 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    20521 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    20581 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    20641 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    20701 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    20761 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    20821 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    20881 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    20941 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    21001 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    21061 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    21121 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    21181 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    21241 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    21301 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    21361 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    21421 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    21481 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    21541 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    21601 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    21661 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    21721 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    21781 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    21841 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    21901 cgcttggcag cacaaataga agtgtacgaa ttaa
//
LOCUS       DAYYNG010000033        21439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000033 DAYYNG010000000
VERSION     DAYYNG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.033"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..582
                     /locus_tag="ACHBFA_001017"
     CDS             <1..582
                     /locus_tag="ACHBFA_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9394527.1"
                     /translation="FYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTLEQLKEA
                     NADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYELGEEKQF
                     GEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRVSVVIPG
                     AKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(671..1930)
                     /locus_tag="ACHBFA_001018"
     CDS             complement(671..1930)
                     /locus_tag="ACHBFA_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM9394528.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(2101..2547)
                     /gene="dtd"
                     /locus_tag="ACHBFA_001019"
     CDS             complement(2101..2547)
                     /gene="dtd"
                     /locus_tag="ACHBFA_001019"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9394529.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(2558..4771)
                     /locus_tag="ACHBFA_001020"
     CDS             complement(2558..4771)
                     /locus_tag="ACHBFA_001020"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM9394530.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(5004..5768)
                     /locus_tag="ACHBFA_001021"
     CDS             complement(5004..5768)
                     /locus_tag="ACHBFA_001021"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9394531.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(5769..6716)
                     /gene="prmA"
                     /locus_tag="ACHBFA_001022"
     CDS             complement(5769..6716)
                     /gene="prmA"
                     /locus_tag="ACHBFA_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9394532.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(6729..7217)
                     /locus_tag="ACHBFA_001023"
     CDS             complement(6729..7217)
                     /locus_tag="ACHBFA_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9394533.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(7183..7854)
                     /locus_tag="ACHBFA_001024"
     CDS             complement(7183..7854)
                     /locus_tag="ACHBFA_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM9394534.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            7992..9278
                     /locus_tag="ACHBFA_001025"
     CDS             7992..9278
                     /locus_tag="ACHBFA_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9394535.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            9350..9544
                     /gene="ssrS"
                     /locus_tag="ACHBFA_001026"
     ncRNA           9350..9544
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHBFA_001026"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            9622..9906
                     /locus_tag="ACHBFA_001027"
     CDS             9622..9906
                     /locus_tag="ACHBFA_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394536.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            9979..10458
                     /locus_tag="ACHBFA_001028"
     CDS             9979..10458
                     /locus_tag="ACHBFA_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9394537.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            10681..10824
                     /locus_tag="ACHBFA_001029"
     CDS             10681..10824
                     /locus_tag="ACHBFA_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM9394538.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            11169..11297
                     /locus_tag="ACHBFA_001030"
     CDS             11169..11297
                     /locus_tag="ACHBFA_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394539.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            11402..12985
                     /gene="abc-f"
                     /locus_tag="ACHBFA_001031"
     CDS             11402..12985
                     /gene="abc-f"
                     /locus_tag="ACHBFA_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM9394540.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(13422..14606)
                     /locus_tag="ACHBFA_001032"
     CDS             complement(13422..14606)
                     /locus_tag="ACHBFA_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM9394541.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14631..15638)
                     /locus_tag="ACHBFA_001033"
     CDS             complement(14631..15638)
                     /locus_tag="ACHBFA_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9394542.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(15810..16169)
                     /gene="comGG"
                     /locus_tag="ACHBFA_001034"
     CDS             complement(15810..16169)
                     /gene="comGG"
                     /locus_tag="ACHBFA_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9394543.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(16141..16566)
                     /gene="comGF"
                     /locus_tag="ACHBFA_001035"
     CDS             complement(16141..16566)
                     /gene="comGF"
                     /locus_tag="ACHBFA_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9394544.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(16529..16876)
                     /locus_tag="ACHBFA_001036"
     CDS             complement(16529..16876)
                     /locus_tag="ACHBFA_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394545.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(16848..17318)
                     /gene="comGD"
                     /locus_tag="ACHBFA_001037"
     CDS             complement(16848..17318)
                     /gene="comGD"
                     /locus_tag="ACHBFA_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9394546.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(17293..17571)
                     /gene="comGC"
                     /locus_tag="ACHBFA_001038"
     CDS             complement(17293..17571)
                     /gene="comGC"
                     /locus_tag="ACHBFA_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9394547.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(17568..18617)
                     /gene="comGB"
                     /locus_tag="ACHBFA_001039"
     CDS             complement(17568..18617)
                     /gene="comGB"
                     /locus_tag="ACHBFA_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9394548.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(18574..19503)
                     /gene="comGA"
                     /locus_tag="ACHBFA_001040"
     CDS             complement(18574..19503)
                     /gene="comGA"
                     /locus_tag="ACHBFA_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9394549.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            19635..21389
                     /gene="ade"
                     /locus_tag="ACHBFA_001041"
     CDS             19635..21389
                     /gene="ade"
                     /locus_tag="ACHBFA_001041"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM9394550.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
BASE COUNT         6298 a         4506 c         3885 g         6750 t
ORIGIN      
        1 ttttatatcc actttccgga tgaaagcaca ccaaaaaatg aatcagttgc tacattgcac
       61 gaattaaaag aagcaggaaa aatccgagca gtcggtgtgt caaacttcac gttagaacaa
      121 ttgaaagaag cgaatgcaga tggatatgtc gatgtagtag aagataaata cagtttgatc
      181 catcggcagg ctgaaaaaga attattccct tatcttgaaa aaaacaaaat cagttttgtt
      241 ccctattttc ctcttgcttc cggtttgctg acaggaaaat atgaacttgg tgaggaaaaa
      301 caatttggtg aaggagatcc tcgaaaaaga aatccagatt tccaaggaga acgtttcaga
      361 gaaattttaa ccgctgtcga tgtacttcgc cccattgcta aaagatacca agccactcct
      421 gcgcaacttg ttttagcttg gtacatgaag aatccacgag tgtctgtagt tatacctgga
      481 gctaagcgtc cagagcaagt atctgacaat gtccaagctt tagatttgca tttatccaat
      541 gaagattatc aaaccattga tgaagcattt cgtggatttt gatcaattga ccttatcaat
      601 ttatagtaat atgaaaaaag gaagatcagc actgtttgat cttccttttt ttcgataagt
      661 ttatgctaat ctaattcttg taaagtagcg ctcggctatt cggataagtc ggaaactgat
      721 tgaatggaat cgtccaatct tgttcaggta cagagaactc aaacttattt actagctgat
      781 cggaaaacac acgtagacta tgaagagtga tccattctcc tgcacagcga tgcatttgtc
      841 gaaaatttcc cccgccttgt gcaatcatct catattcttc tttataggaa atatcttttg
      901 ctttccctac ataccgtttg atcataaagg aatctggcgc ttcaactgtt cgttcatcat
      961 ggtctgttcc atataaatct aaaataaccc aactgccttc tggtatacga taaccatcta
     1021 cttctacttc tttcagcgaa atcgcaggta gcattgggaa gaaaggataa tagcgtctca
     1081 tttcttgaat aaacggatct tgtaacgtcg aaaagtcatt ttttaattgc tgatacaaat
     1141 ctggtctact gaaaagtgca tgtcccatca atgccgccca cacagttaga gcaaccgttg
     1201 gacggatgat gttcaataat tcaacagctg ccacttccag aggtaaaagc tgaccatcta
     1261 aatctgtggc attggcaaaa gcatataatg ccacattttc ttttccagcc acaggatttt
     1321 gtcgagcttc ttcaatcaag ccttgcgccc atttttctga ttttttgcga ttttcgacac
     1381 cttttatgtg atcaatcgga gaagtaaacg ttccgctgat catagagatt tgatattctg
     1441 ctaatttttc gacctcatct ttagaaatcg ctgataaatt gatacctgcc cactcgcaaa
     1501 tagatgtaaa gaaaaccatc ttacttaaat caaatagttc aaattgacca tgctgtgctt
     1561 caagtgcttg tgtcagattc ttatctaaga tacgatgata atcttccatt ctttccggtg
     1621 tcattagatc cataaaaata tttttccgat ggtgatgagc agctccatcc aaagtctgta
     1681 ctccaccttg accaaaaagt gtcttcaata ctaattttgg catagcacct tttcgtttga
     1741 aattccttgg atcataaaat acttttgctg ctgatgatcc gtagatcgtg atagcttctt
     1801 tattgaaaat ctttgctttg acgacgggag cgtccgcttc atggcgtaac tcctccaaca
     1861 tgttgtatcc tttttgatac aattttttta aatctgtgat tttgatatct actactggta
     1921 cttctttcat cttcagcaac ctcctataaa aaataacttg tataatttaa gcttaacttt
     1981 ttttccgact gctgacaaaa gaagcgcttt ttgcagacaa aaaagccact taagttagag
     2041 tttatcagtc taacttaagc gacgtgtttc atagttttta ctagtgaaaa ccagattact
     2101 ttatttgttt ttagaatcaa taatgatcgt tacaggtcca tcattgacga gagaaaccga
     2161 catatctgct ccgaattgtc cggtagcgac tgtgatgccg cgacttctga ttccttcatt
     2221 gaatgattcg tataaaggaa tagctgtttc tggtcgtgcg gcttccacaa agcttggtcg
     2281 atttcctttt ttcgtatccg caaacagtgt gaattgagag atacttaaaa tttgtcctcc
     2341 cacatcttct acactaatat tcatttttcc ttgttcatct tcaaaaatac gcatttgtgc
     2401 aatttttttt accagatagt ccacatcttt ttgcgtatcg gtttcatgaa tgcctaataa
     2461 aacaagtagg ccgtagccaa tttttccaat ttcttgctga tcgactgaca cagcagcttt
     2521 acttacccgt tgaacaactg ctctcatatc tgtcctccta tccattcgtc cggcgtacac
     2581 tgtagacatc aggtactgct ttgattttat ccacgatact ctttaggtgg gataaattct
     2641 ggataccaac cgtcaaatgg atcgtcgcca ttttatcttt attcgtccgt gcttcaacgc
     2701 tgttgagatt tttcgttagc gcattgactg tttgaagtac atcattcaat aagcctgaac
     2761 gattatagcc gtaaatttcc aaatctgcat catactcttt tcgtgtattc gatgtatctt
     2821 cccattcgac ttcaatcaaa cgttctgcta catttggttt gtcaggctga acattcggac
     2881 aatctcgacg atgaatggat atccctctgc ctttagtgat ataaccaacg atatcatcac
     2941 caggaatcgg attacagcag cgactgatac gaattagtaa gttttctacc ccttgaatga
     3001 caacgccacc ttcatgacgt actttcattt tgtcaggttc ttttttcggc tgattcatga
     3061 tttcttctgc ttcttgcttt tgctgctcga ttttttgttc tttacgttct ttttcagtca
     3121 gacgattggc catcgtcaag gggctaactt ctccataccc tacagctgca tagagatcat
     3181 cttctgtttg ataattaaaa cgatcgagtg cttcttgcag tttgttcttc gtcaaaatat
     3241 ctttaggcgt aaatcctaga tctgtaatac atttgaccac ggattcgtgg cctttgatca
     3301 cattttcttc tcgatcttgg gctttgaaga aacgtttgat cttatttctt gctttgctag
     3361 tagcaactaa tttcaaccag tcgcgacttg ggccaaatga atttggagaa gtcatgatct
     3421 caatgatatc tccgtttttc aatttgtaat caagctgcac cattttgcca tttacttttg
     3481 caccagtggt tttgttaccg atatctgtat ggatactgta tgcaaagtcc aatggtccgg
     3541 atccttttgg taactcagta acgtctcctt ttggtgtgaa gacgtagact ttatcactaa
     3601 agatatctcc ttttacgcct tccataaatt cagaagcatc atagctttcg tcttgaagtt
     3661 cgagtatctc atggaaccag cttaattgtt tcgtcatacc atctggttct actttttcat
     3721 tttttccttc tttataggcc cagtgtgcag caaccccgaa ttcagcgatt tcatgcattt
     3781 cctgtgttcg gatttgaatt tctaccggat ttcctgccgg accgatcacg gtagtatgca
     3841 aagattggta catattagct tttggcatgg caatataatc cttaaatctg ccgggcattg
     3901 gtttccattt tgtgtggatt gctcctaata cagcataaca atccttgatt gaatctacga
     3961 tcactcgaat agctagcaag tcataaattt cgttgaactg ctttttctga tctttcattt
     4021 tacgatagat tgaataaatg tgttttggcc gtccatagat ttctgcaaaa atccccagct
     4081 cttctgtcgc tattcgaata tcttcaacag taccacttac gtatttttca cgttcttctc
     4141 tcttcgtctg catcaagtgg acgatacggt aatactgttt tggatttaga taacgaagtg
     4201 ctgtatcttc taattcccat ttgatccggc tgatccctag acgatgtgca agaggcgcat
     4261 agatttctaa agtttcctga gcgatccttc tttgcttatc ttctcgcagg tgcttcaacg
     4321 tacgcatgtt atgtagtcta tcagctaatt tgaccatgat aactcgtaaa tcttgagcca
     4381 ttgctagcag catctttcgg tggttttctg ctagctgctc ttcgtgggat ttatatttta
     4441 ttttccctaa tttggttacg ccgtccacta acatagcaac gtcatcgcca aattcttctc
     4501 tcagatcttc taaagtgatt tctgtatctt caacaacatc atgtaagaag cctgtagcca
     4561 ctgtatgggg atccattttt aaatctgcta atatacctgc gacttggata ggatgaataa
     4621 tgtagggttc gcctgatttt ctaaactgtc cgtcatgtgc agcagtcgcg tattcgcatg
     4681 ctttctcaac aaatgctaca tgttcgggac ccatgtattg actaacgagc tttatgacac
     4741 cgggtcccgt taaaatttct tcttttgtca caggaatcga tccccctttc ttttttcggt
     4801 aaaacgggaa tcgggacaaa tatctgtttg ataattgtcc caaaatccgt gcagttatac
     4861 gtttttttac ggaaatgttc gctttttaaa agctttttca aacgattttt cgtatccatt
     4921 gtataaataa ttgtttctat tatacaaatt gttgactatt ttttcaattc tttctgtcga
     4981 tactctttta ttaatgaaga acgttagctg aaagaaggat tcattaattc ccattgatag
     5041 ctagcagcac ttaataaata aagaggtgct gtttctgtac gaaggattct tggtcctaat
     5101 ccacaaagta tccctttgtg ttctacaaaa taatcgactt catcgcggga taatccacct
     5161 tctggtccaa agacagctaa gattcgacta tctggttttg tttcactaag aattttggct
     5221 gcatttgatt tttcgccttg ctttgctgat tcctcataag caaccaagac cacgtcataa
     5281 tcatctagcc gctcaaataa atcttgtttc gtttccaata actctactga tggcacgatc
     5341 tgccgatggg attgttccgc tgcctcttga gcaatctttt caagccgttg ttgtcgtttc
     5401 atacgttttt ttgcatccca tttaacgaca gaggctgatg ccgggaaacc aagaaaagct
     5461 tgcgctccta attcagtgga tttctgaacg atccaatcca gcttttcccc tttaggatag
     5521 ccactcgcta tggtaatcga acttggcagt tccttttgat tttcttcttt tttaatttct
     5581 ttcagataaa cagcctcttc agtaatctcc gtgatttctg caacaatggc tatttgatca
     5641 ttgaatgcca aaaaaacttc atcctcgggt tccatcctca taacccgtac aatgtggtga
     5701 tagttttccc cttctaaaac aaaatagtct tttgattcat atgattcttt taaaaaatac
     5761 cgctgcatct attccacctc tggttttttc aaaataattg cataccagtc tttttgctgg
     5821 aaaatttgat ccacgataaa cccttgttct aacatggcat ccagaaccat ttgcttcttc
     5881 tcttcaatga ttcctgaaac gatcagcgtg cctgtttgct tcagtaaacg ccaagcatct
     5941 gggatcatca ataagatgat atctgctaaa atgttcgcaa cgattacatc actttcaatc
     6001 tcgattcctg tcaaaagatc gttagctgat acatggacat ctttggctat ttcattcata
     6061 tccatatttt ctttcgccga acgaacggct acttcgtcta aatcaaaggc atagacatct
     6121 tttgcaccaa gatattttgc tgcaatactt agcacgcccg acccagttcc cacatctagc
     6181 aatgtttcac caccacgcaa gactgtttct agtgctgtca atgtcaagga agtcgtggga
     6241 tgagtgcctg tgccaaatgc cataccagga tctagtgtga taattttttc tagaggatct
     6301 tctgtctggt agttttccca gcttggcgca accgtcagca ttcgcgtaat acgcaccgga
     6361 tgatagtatt tcttccaggc tgttgcccag ttgctttcct ctacttcact aactgttact
     6421 ttattttttc caatagctag accaaactca ggtaattgtg tgatacgatc tttgataaaa
     6481 ggcaagattt ccggtaagaa agtcgtttcc ggaaaataag ccatgactag tgcgccttct
     6541 ttcagcgatg taaaatcatt tttatctaaa atctcaccat attgatccga ctgaaaatgt
     6601 tcaacatcta aagaatcttc aatcgcgaca ccactcgccc ctgcctccat caaaatatta
     6661 gcaactgctt cgactgcttc actttcagtt tctactttta cttcattcca tttcatatct
     6721 gtcactcctt aataacttgg gtacggtaaa taagtatctg cttcattttg ctgttttact
     6781 tctactaatt ctgctagttg ctgattatcc cagtgaagct cgaatacagc ttcgtcatcg
     6841 tcttcatcca aaaaatcaag caaatcgctt tgcccttgag ctaatacttc atttagatat
     6901 tccactaaag aatcagctac tgattttgcc aacccttttt ttccttcata agggattact
     6961 gctagataat tgcgttcatc aaaaacagat ttctgaggat taaaaagaag tacgccgtct
     7021 tcaaattcga taattggttc ttgcgaaacg atcccttctg catcatcaat ctcaaattgt
     7081 gctttatttt ctgcaaacaa gcgaacaacg atctcgatcg tatgattttt tgtatcccaa
     7141 tctaaagcta catcatagtc tgtcacttgt ttttcaatta tttcatctaa atatgttaat
     7201 agatccttct ttttcatttt ctttcctcca accccaatct ttctcgattt ggttttttct
     7261 ttgtaaagag atataaggat ttcccgacac aacaaaacgc aacggctttt ctgtccatac
     7321 acctttgttt gggataccga ttctaggcaa agcattgatc ttttttggtg tctttttttt
     7381 ctcaaaaact aagcgtaacg aactatctgc tatagactga ccataaagtt cttgaggaat
     7441 tgctaatgct tccactaatt ttcccggacc gttgctgata tctggaccag tttttccgcc
     7501 acgattttta ctcatttgat caatcattgc ggcgggttcg attgcacgta tcattactcc
     7561 ttgaggaatc ccttctggct gagtaatgat gttcaaaatc cgatgagtat gcatcgtgta
     7621 aaggtagatc gtacctggtt tttcatacat tgcccgcaca cgcggtgttc ttcttaaacc
     7681 atagctatgg gcggcctcgt cttctggacc taggtatgct tccgcgtcta caatatatcc
     7741 agctaatctt ccttctggtg tgtcatgctc caaatacatc cccaataaat cccgtgccac
     7801 ttctggcgtc gttttattcc aaaaaaattc atctatcttt tgtttatccg tcatttttct
     7861 cacctacttt atcatacaca atattagttg gataagctat gaaaacaaaa caacagaagg
     7921 ctgttctttc taggcatctt gctaaatatg atataattaa gggacgtaaa aacaagaagg
     7981 aggaaaatcc tatgcaacag ccattggctt atcgcatgag acctcgaacg atcgatgaag
     8041 tcgttggtca gcagcatcta gtcggggaag gaaaaatcat acgccgcatg gtcgatgctc
     8101 gcatgctctc ttccatgatt ctatatggac ctcctggtac tggaaaaacg agtatcgcaa
     8161 gtgcgatcgc cggctctact aattatgcat ttcgcatgct aaacgccgct acagattcaa
     8221 aaaaggatct acaagttgta gcggaagaag caaaaatgag tggtacagtg atcctcttat
     8281 tggatgaagt acaccgatta gataaaacaa aacaggattt tctgcttcct catttagaaa
     8341 gtggccgaat catcttgatc ggagcgacta ctgagaatcc ttatattacc atcaatccag
     8401 cgatccgcag ccgtacacag atctttgaag tcaaaccatt aacagaacaa gacatccagt
     8461 tggcagtcga acatgcttta agggataaag aacgcggcct tggacaacaa gcaatccagt
     8521 tagacgaaga tgctcttctt cacctttcgc gtgcaacgaa cggcgatttg cggagtgcat
     8581 tgaatgggct ggaactggct acactatcga ctccttcaga taaagaagga aggattcatc
     8641 ttacactgtc gatcattgaa gaatgtgtcc aaagaaaagc tcttacccat gacaaaaatg
     8701 gagatgctca ttatgacgtt atttcggctt ttcaaaaatc gattcgtgga agtgatgtgg
     8761 atgctgcact tcattatctc gcccgtttag ttgaggcagg tgatctggca agtatttgcc
     8821 ggcgtctgat ggttatcggt tatgaagata taggtttagg taatcctgct gctgcggctc
     8881 gtacagtgaa cgctgtatta gctgctgaaa aattgggatt gcctgaggca cgtatcccac
     8941 tagcagacgt cgtcgtcgat ctttgcttgt cgccaaaatc caattctgcc tacatggcat
     9001 tagatgcagc acttgctgat attcgagagg gcaaagctgg agatgttcct gatcatttga
     9061 gagatagcca ttacaaaggt gcgaaatcac tcaaccgtgg cgtgggttat cagtatcctc
     9121 atcattttga tcaggcttgg gtcaatcagc aatatttacc agataaactg aagaatgctc
     9181 agtattatca gccaaaagat acagggaaat atgaacaggc tctgggtcaa cagtattacc
     9241 gtataaaaga gtggaaaaag catcctccaa aaaactagac aagaaaacgg ttctgtttct
     9301 gcgagctctc tcattgctct ttttaaattc atatgctact atagaggtgg gaaatctgtg
     9361 atgttcgaat tgtcctcttt gtgtgttgac cgaacatttt tattgatatc ttgggacccg
     9421 tcttgtgtca ctgctggtaa catgccttat catttggtag ttcgaagcta cgaccatggg
     9481 acccacctgc ttgaattcgc gggatcaata ctacaggaca aacaacggca ttaacggaga
     9541 ttaccatctt ttaaagaata aagcaatttg ctcttggcaa attgcttttt ctatttgttg
     9601 cttattttgg aggatttgtt catgattagg attttaatga ttatcgccac tttagtatta
     9661 ttattcgtta gctattattt atttaataaa caggatatct tttttgtatt gatcaacaaa
     9721 aatgataaaa atcaaggatt cctacaattt tacggagctg catatgctgt tttaggcgta
     9781 atgggtattt taacagcttt tttcaatcaa cgctttattg ccttggtctt tttactgatc
     9841 gtaattattg tttctgccac cttcagtatc cgatttgcaa aaaaaattgc tgaaccaaag
     9901 cagtaaataa ttctcttttt ccctaaagtg ttctacaata aaagagtaca atacgattga
     9961 cttgaggagt gaatgcttat gttacaacaa tacaaaaaaa tcatggttgc cgtagatggt
    10021 tctgatgaag ctgaattagc ttttaaaaag gcggtcaatg tagctatccg aaataacgga
    10081 gaacttctat tagcacatgt cattgacaca cgttctttcc aaaccgtttc atcctttgat
    10141 ggaatgcttg cagaacaagc tactgaaatg gcaaagcaaa cattggcaga ttatgaaagc
    10201 aatgcgaaga aagctggctt gaataatgta acctctgtcg tagaatatgg ctcaccaaaa
    10261 caaattattg ccagagaaat cccagaagat aatcaagtag atttgattat gttaggagcc
    10321 acaggattga atgctgtaga acgtttattt attggttctg tttctgaata tgttattaga
    10381 aacgcagctt gcgatgtgct tgttgtccga actgatttag aaaaccaatt gccagctaaa
    10441 gaccacacca aagcttaaca cgtagacgac ctgccaacta tcggcaggtt tttttgcaca
    10501 aaaaaccgtg ataaaaacaa aagacgcggc aagcactcat cggtctttat aatgtttaaa
    10561 ataaagggaa aacagtcaat gagttcaagt aaaataaaaa aaagtgaaca ccagggcgat
    10621 tagtcgccct ggtgtggttt aaaacgacct aagcatgcca aagcttattg gttcgtcaat
    10681 gtggaagttt ccgatgaatc tgatacgaaa aaatatgaag tatcgatggc ggataccgat
    10741 tttatcgcca aactaaaatt ttatttcggg ttcgggtacc gcgagaaggc aagtgctaca
    10801 ttcggtaaat cattcaagaa ttgatgaaag gctaccgctt gccattgcaa ccacaacgat
    10861 actcctgaac gcgatgttgg tgagatttcc aaagagaaga gctgtttaat gcaacggtat
    10921 taagcaaatg gcaaaaggaa gtcatgtaga caaagttttt tatcaaacac catatgattt
    10981 cgcctgaacg ctggtttagc tcagaataca atgatgtggt ccacgagaca ttaccctcag
    11041 tcagataaaa aaagctctct gacagaatta cacatcgata aatattaagt aaatgtcagc
    11101 gctagttgaa ctttctgaca ctttcaggta cactggaaaa gacaactaca cgaaggaggg
    11161 aaaatactat gatgcgcatg actaaaataa caagtaatga aaattaccaa cgataccata
    11221 tcgcatttag ccatgcaatt ggttttacag ttgctgttga cgggacaagt ttgtccaaaa
    11281 cagttgaatt cacgtaaaat caatggccat ctcaaggtat ttttctcatg ataatctgag
    11341 tttaaataac acaggccatg gataaccatg gcctttttga gtggtctcaa ggagagagat
    11401 catgatcata caagcaattt ctattaaaaa aacaatcggc gggaatccat tatttgagga
    11461 tttatcctgt caaattgaag ctggtgaaaa aattgcctta gtcggtatca acggaactgg
    11521 caaatcaacc tttttacaaa tcttgaccgg cagagaaggt gtcgacagcg gtgttatcag
    11581 tcgcaaaaaa ggcttaaaga tcgggacggt cgaacaagaa cttacagttt ccgaagcgac
    11641 cgtcaaccac tacttgctcc attctgccgc tgagatacag gacctcaaac agcaaatgag
    11701 tcgctacgaa gcattgatga cagaaccaga tacaaactta gagaagtgtc tggcccgtta
    11761 cggtgagctg cagcatcgct ttgaagaact aggcggttac gtattagaag accggattgc
    11821 agcgatcttg caaggcttag ggattccaca ccgtcaggaa gcacggttaa cagaacttag
    11881 cggaggcgaa cgagttaaag tcgctttagc caaaatttta gcagccgatg ccgatttact
    11941 tttactcgat gaaccgacca atcatttaga tcttaacagt attcgctggt tggaaaacta
    12001 tttacagaat acaaaaaaat cggttctggt cgtttctcat gatcggcaat ttctcgatca
    12061 agtcaccaca aaaacatggg aactggaaga aggtgcattg attgagtatc ccggaaacta
    12121 cagccgtttt cgagtcttga aagaagctcg cttggctgag ctgacaaaaa attacgaact
    12181 gcagcaaaaa gaagtccagc ggttgaaagt catgattcgt cgttttcgtc agtgggcaca
    12241 tgaaggagac aatgagtcct tcttcaaaaa agccaaagaa ctggaacgcc gcctggcgaa
    12301 actgacactc gtcaaaccgc cacctccccc gaaaaatcgg ctgcaaagct tgtctaatgg
    12361 tggaaaatct ggcaaagaag ttttcatcat tcaaaacttg caccagcagt acgctgatca
    12421 agtactattc aaagacagca gttttgcagt ctatcgtgga gatcatttag ccatcatcgg
    12481 cgacaatggt gcaggaaaat caaccttact caaattatta ctcggtgagg aaaagcctta
    12541 cggtggcacc atcaaattag gtagtagctt gcaaatcggt tatttgccgc agcagctgca
    12601 gtttgccgat cccgatagcc gtttgctggc ttacgccatc acattgactg gaaacgaaga
    12661 agtcgctcga cggcaattag ccaaaagtgg cttttatcaa agcgatgtgg gtaaacggat
    12721 caaagattta tccggtggtg aaaaaatccg tttggcgttg ctgaaactct ttttagaaaa
    12781 aatcaacgtc ctgattttgg atgaaccaac caatcacctc gacagctatg ctcgagaaga
    12841 aattgaagaa atgctgcaag actatcaagg aacgctccta gcagtcagcc atgaccgcta
    12901 ctttttgcag caacacttcc aagaagcact cgttattgat caggagcaga tcagccgcca
    12961 gccgctcggc caactgattg actaggacgt tttttcgcaa caaaaaactt gattttcata
    13021 cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat caagttttta ttattcgatc
    13081 aatgaatgtt tcggatcggt tttctagcta aacataaagc gaagaataaa gtcgacagcc
    13141 cacgaggctg atctaaaaat gttttttctt tttacatatg taatgtccct tataaagatg
    13201 aatgaaaata aaatattggg attttcctga aaatcaataa ataaaatgcc agtcttttcc
    13261 cttttgacca cttttccaag caaaattccc taagaagatc ctgtaaaaaa gaagggaaat
    13321 tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa aacacatgca agaattccct
    13381 cttgcatgcg tttcatcttt tatcctaacg aatgctttgg cttagccgcg cagacgctcc
    13441 acatcacggg caatcattag ttcctcatct gttgggatca ctaatactct tacttttgat
    13501 tcttttgtag agatatctac ttcttcacca cgcaattcat tcaacgctgg atcgatttca
    13561 caaccaaacc aagacatacc tttgatgatt tcactacgta catgtccgtc attctcgccg
    13621 ataccagcag taaagacaat tgcatcaaca ccattcatcg ttgtcacata accgccgatg
    13681 tattttcgga tgcggtccac gaaaatgtca taagctaaac gaatatgttc tttttcatag
    13741 ttcttttcaa gatcacgcat atcactagac aatcctgtta ggcctagcag accagatttc
    13801 ttgttcaaaa cattgatcat ttcatcaata tcaatctcta atttttccat taaatatggg
    13861 agaacagctg gatcgatatc acctgaacgt gtacccattg tcaccccagc taatggtgtg
    13921 aatcccatag aagtatccat ggatttacct ccatcgactg ctgtgatcga tacaccattt
    13981 cctaagtggc aagtgatgat ttttagttct tcgatcggtt tgttcaataa ttttgccgca
    14041 cgttccgaca cataacgatg acttgtgcca tggaagccat attttcttac tttgaaatct
    14101 tcataataag acaccggtaa gctatataaa taattttgct ttggcattgt tgagtggaaa
    14161 gatgtatcga atacagccac actcaaaatg tcaggtaaaa tctttttaaa cgctttgatc
    14221 cccattaagt ttgcaggatt atgcaaagga gcaaattctg ctaattcagc aatgcggtcc
    14281 attactttct catcgatcac tactgaatcc ccataagttt ctcctccgtg aacaacacgg
    14341 tgccctactc cggtgatttc atcataagag cttaaaattt gcagttcaat caactgatct
    14401 aacagcattt ttactgctac ttcgtggttt tcgatatcca tgatttgttc gaatttttga
    14461 ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac cgatacgttc gacgatccct
    14521 ttcgcaacta cggtttcttc tggcatttga tataattgcc atttcaaact tgaactccca
    14581 gcattgattg caattgtttt agacatatga ctctccttct cgttgaatga ttacagagca
    14641 gatgatttcc agtttttaaa ctcgttgaaa aattccgtca ccttctctgg ttcttttaat
    14701 gaagcaagtt tcacaagcaa gacttctttt acttgttgtg cttgtggtcc ttttttctgt
    14761 aagatcaaaa tacttttttg cgattgttta ttacggaaca attcatctgg caattgaatc
    14821 attccttgaa gataaccttc ttctttaaac cattttttga tttcttcact ttgatccgtt
    14881 tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat ccggcttcac atatttcatt
    14941 gattgctcta gcaataaatg atgtgcataa ctatggcctt cttcggtact tgttaaaaac
    15001 tcttttgctt tttcgtcatt cggataataa ccgattggca aatcgctgat tgcaaaatcg
    15061 acaggatcta ttaataattc ctgcaacccg tcttgatgaa agtattgaac atttgcttgt
    15121 gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat catcaatatc cacaccaatt
    15181 ccttctgttt ggtaccccgc attcgatagg tttaaaagaa ctgttaataa caagttgccc
    15241 attcctgccg caatatccag aatcttaacc ggtgcttttt tatttgtgta tagttgttca
    15301 ataagaaaaa caaataaaaa accgatacta tcaggagtga gttgatggtt cgcctgcaaa
    15361 tgttctgtct ggctgccctt caataaaagt aattgagaca gtctgcgcca ttcttctggc
    15421 tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga tccgttgcgt tgtctctttt
    15481 gtcggcactc catccaccac acgtacttga taatcatcta ttaaattttc tgcattttcg
    15541 acataggctt ctaaaaagga agttcccaat gcattctgta aaagctgaat agcttccagg
    15601 ttttgattaa acccttgttc cattttttct ggtaacatgg tgcacctcat ttctagatat
    15661 attataacaa ttttttcacc tgtttaattc tatcgaacaa ttgacaaaat ttcaatcgtt
    15721 ttcactgttt tttatttgtg aaacggcaaa cattttttta ttaaaaacag tgttataaca
    15781 gaaaagaggg cgttttcacg aacattccat cattctgtac tagtatctgt atcatttttt
    15841 acttcttctg tgaaccgaaa gataaacggt gagacttcca cctctaagaa tagggactgt
    15901 tgtacccact gatatgttac agatccagtt gtgtagtaaa aaactccttc cgaactttcg
    15961 gcaagcgtag actgttccga taaagccatc tgcttcatta tttttgctat ataataattt
    16021 ttgattcgta tattaagatc cattgacaaa cggtagcttt ctaatgctgc catcaataaa
    16081 aacgtacata aaagaaataa aagcaagaca gaaaataaaa tagagccctt ataacatttt
    16141 ctactgatcg ttttttgtcc atatggcata tccatcttct ccattttcaa agtgtacata
    16201 aaaagaaatt gtgtgttctt tttccaataa ttgaagctca gtgatacttg ttaacatagg
    16261 ttgatgtccg ccggaagttc ttcttttacg tatcattgcc tgatatcttt cgatgatata
    16321 ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc acagagacat tgtgactgct
    16381 cttgagttct tcttctaatt gtgctaaaaa tattagccac tctttttgat ctttacgctg
    16441 caaaaattca cttacttgga tggtttgctg aatcaaaaga ttgaatgtga gcaatagact
    16501 gctcaatatc atcagtccaa ttagacattc aatcagcgta aatgctggaa ttacttgttt
    16561 tttcaatact gatcgcttct ttctccatat tgacaaacac tttttcgatt actccattat
    16621 tatcgacaat ttggatatcg tatgggatca tttcttccct tcgctctggt cttttgtgat
    16681 acatccgctg cgtcttgata tcttcataaa tcattcgaat aaatagcagt tcctccccac
    16741 ttttcttttc tgcctgaagc aaaaacagct ggccaaagct cactaagtag acacagacag
    16801 aagcgatcat aagcgctgta agggactcca acaaaacaaa tcctgtttta ttcaattctt
    16861 tttgtatagc gaccactccc tatctggaat tgatacgtaa tcgtccgctc tttttcttcc
    16921 caataaaatt gataagcttt tagtgaactt tcattcccgg ttctagcagc aaaagtaata
    16981 gaagcatggc gtttcaaggt aatctcatca ggaatatcta atacttccca atttatttta
    17041 tctggattcg gaacatgaaa aatgatttga ttctcttcac tattccaata aaagcctgtt
    17101 tggatctgat tcacaatagc gattttttgc gtagaataga ttcgtttttc aaattggccg
    17161 aaaaattgat agactgccaa ttcattcttc catgcagaaa atgcgagaac tggaaaagaa
    17221 aggatcgttg taagtaataa aatcagtaag agtgattcaa acaacgtata tcctgatgct
    17281 aaaaccttac cttcaaggcc gctgatagat ttctgcatgt tctcgtttga tatagccttc
    17341 attcaacaat tcctccacag taggaactct tccattattt tgaagcgcat agagctcttt
    17401 ttgtgtgtct accaactgga tgatcgcttc acgactttct tggtctacat gattcctata
    17461 acgcgacaaa ttagggacga acaacaacac taatacactg atgatcagta agacgattag
    17521 catttcgatc aatgtaaaac cgctataata agatattttt ctctttttca tggcaaaacc
    17581 tcctctatgc ttccgtaaac aggtaataaa acagcggcat aaactaataa aatcaaagcc
    17641 gctacgccca aaaaaatgat tggctgaata aaatgcatcc agtgatgaag acgtttgaaa
    17701 agctgttttc ttgtccattc gctgtaaaaa atcagttctt tacctaaact tccctttgcc
    17761 tctccctgta aaacaatttt actgaattct tctgatagaa aacgataatt ctggaactgc
    17821 tcagccaatg acatacctgc ctccaatcct tgaatcaact tagaggcaag atgctgaatc
    17881 agactttctt gtttcgtttc ctttaagcag actatgattt gccttaattc gaaaccttca
    17941 tgaaaaagct ttccaatctc tagtgacaaa taagccgatt gatacaagga atatatcggt
    18001 ccaatcaata attgtctgct tatccattca ctctgatcaa caggagacaa cttcatcaga
    18061 cgatacctta ctagtaaagc caatagagta accgctagga tgaatagtag aacataccaa
    18121 tgaaagactt gtatcagctg aataccccaa tgctcttgca tgaccattcc tgaaccaata
    18181 agctgaggca aaacgaacaa ccgcatacta aacaagatac ctagcaaaaa ggtgagcaat
    18241 agcatcggat atgcaagcag ctttttcaaa tcattgcgaa atccttcaac aagccgaaac
    18301 tgtttggcaa tttccaacaa agttgtcggc agattcccat gacgttcagc taactcgatc
    18361 tgtaccagtt gctctgctcc atatcctatt tgcgaaagta cctcactaaa cgcttgccct
    18421 tcctttagct gatgttggat atttgccaaa ataacaggtg gaaacgaacg gatcgtgagt
    18481 aataaataaa gggattcttg cagactaaaa ccagataaaa gcagttctcc catggtttgt
    18541 gcaaactggt tttgctggct tcttgttaat cctttagaat agttctgact gccaaatatt
    18601 ttcctgagct tcttcataac tttcctccca tgattttttt actgattctt cctccataaa
    18661 ttgataactc cataaaacag aaggatggtg attggcatct tccaaaagtt cttgataaat
    18721 gacaccagct aaacattcag atagctcttc ttttttcttt gtcagttcat ggattctgac
    18781 caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa acccggtgcc ctgttagtgc
    18841 tgctcttacc gcagcatgtg cagttgattc atctcttatc tcgccaatga ttagcaagtc
    18901 tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa gtctgtgcta ttttcggatt
    18961 gatctgtagc tgcagaaaaa acggttcttc tatctctaca ggatcttcga tagtaatcac
    19021 ttgcaatttt tcttccaaag ccaaccgata cattagagaa gtcttgcctg atccgactgg
    19081 tccgctgaaa aggtataatc cacgtctgac ggttttctgt aaaatagttg caaaatctcg
    19141 agaactaaag caacgatggg gtgtttcaaa agtaccgtat aatagccgaa tcactaaact
    19201 ctctcgttga aggtaatctc ctacagaaga caaccgcaga cgaacttcct gtttttctaa
    19261 agaataagta aatgcaccta actgagcttt tcttttttct ccgatatcca tctgtcccaa
    19321 atacttaaat cttgagatca gttgctgtcc atctgccgaa gagactttct tccagaatgt
    19381 tcgttttttt ccatagcgaa aatagatgta aaatacttct gaagctggca atatatatac
    19441 gtcttgggca tgtttggcaa aactttcact tatcatttga tccgataatc gttttatttc
    19501 cattattctc ccctccacaa aaaacatacg tctttttgat ctttttgttt caaataaaat
    19561 gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt atacttataa aaatttatct
    19621 tgggaggcac tccattggaa acaacattaa ggaaatatat cgaagtagca agtaaaaaaa
    19681 agcctgctga cctagtgatc aagcatgcta agatcgtcaa cgtctttacg aaagaaatca
    19741 tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg aatcggtgaa tacgaaggaa
    19801 aaagcgtaca taatgctaat gggcaatatc tagtgccagg attcatcgat ggccatgtgc
    19861 atatcgaaag ctcactttta tcaccaagcg aattttcaaa agtatccttg cttcatggag
    19921 ttactactgt agtgacagat ccccatgaaa taggaaacat tgctgggagt acaggtcttg
    19981 agtttatgat agaagatgca agacaaacac tcatgaatat cttttttatg ctaccttctt
    20041 gtgtacctgt aacccctttt gagaccaatg gtgcaaattt aaatgcaaaa aaattagccc
    20101 cttttttaca aaaacccgaa gttttaggct tagctgaagt catgaactat caagcagtag
    20161 caaataatga aacagacatc ctagaaaaga tccaattgat gcaccagcat aagaaaaaaa
    20221 ttgacgggca tgcagctggt atcggcatgg aagagttaaa tgtttatcct gcagcaggta
    20281 ttcggacgga tcatgaagct acaactgcaa aagaagctaa agaacgtttg gatcttggta
    20341 tgtatttaat ggttcgagaa gggaccgttg caaaagatct ggcatcgcta ctaccggcga
    20401 tcacacctga aaattcaaga agatgcttct ttgtcaccga tgataaactg atcaatgatt
    20461 tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc tattgcactt ggaatcgatc
    20521 cattacaagc cattcagatg gcaacattga atgcagcaga atgttttgat ttgaaaacac
    20581 taggtgcgat tgcccctggg tatcaagctg atttcttctt aacagacgat cttaccaagc
    20641 tgccaattca tgaagttttc tataagggaa cattagttgt tcaatctggg caactccaag
    20701 accatttatt tgctgatcat tcaaattcat atacgtgttg tctgccaaaa ctaaacgctc
    20761 aaccattaaa tccacattcg ttagagctgc ttctttcttc ttcacaagcc catgtgatcg
    20821 aaattatccc gaatagtcta gtgactaaag atttggtaga aaaagtggac cgacaaggcg
    20881 aacgattcat tccttcaatc gaaaaagacc aattaaagat tgcggtgatc gagcgtcacc
    20941 atcaaacggg aaacattgga ttaggcatag tgaaaggatt tcagctgaaa caaggagcga
    21001 tagcgacaac gattgcccat gactcgcata atctagttgt cgttgggaca aacgacgagg
    21061 atatgctgta tgctgctaat cttttgattc aaaaaggtgg cgggatgacc gttgtcaaca
    21121 aacagcagga aatagcttgt gtttctcttc ctataggcgg aatcatgaca caggaacctt
    21181 tttcgatcgt ctatgaacag ttgaatgaat taataactaa agcacatcaa ctaggagcag
    21241 ctaaaacctt tgatcctttt ttgacacttt ctttcttaac attatctgtc atacccgaat
    21301 tgaagatcac cgacaaagga ttattttcat tttctcgctt ccaattgatt ccaccttgtg
    21361 gaaaaaccga tgaaataaat acattttaaa tcaaataaat cccagaaaat acattacatc
    21421 tgtaatgttc attttctgg
//
LOCUS       DAYYNG010000034        21293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000034 DAYYNG010000000
VERSION     DAYYNG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.034"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..675)
                     /locus_tag="ACHBFA_001042"
     CDS             complement(64..675)
                     /locus_tag="ACHBFA_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9394551.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(798..923)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1005..1721)
                     /locus_tag="ACHBFA_001043"
     CDS             complement(1005..1721)
                     /locus_tag="ACHBFA_001043"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9394552.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1727..2296)
                     /gene="scpB"
                     /locus_tag="ACHBFA_001044"
     CDS             complement(1727..2296)
                     /gene="scpB"
                     /locus_tag="ACHBFA_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9394553.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2280..3050)
                     /locus_tag="ACHBFA_001045"
     CDS             complement(2280..3050)
                     /locus_tag="ACHBFA_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9394554.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3047..3418)
                     /locus_tag="ACHBFA_001046"
     CDS             complement(3047..3418)
                     /locus_tag="ACHBFA_001046"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9394555.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3437..4324)
                     /gene="xerD"
                     /locus_tag="ACHBFA_001047"
     CDS             complement(3437..4324)
                     /gene="xerD"
                     /locus_tag="ACHBFA_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9394556.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4420..4893)
                     /locus_tag="ACHBFA_001048"
     CDS             complement(4420..4893)
                     /locus_tag="ACHBFA_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9394557.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(4998..5861)
                     /locus_tag="ACHBFA_001049"
     CDS             complement(4998..5861)
                     /locus_tag="ACHBFA_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM9394558.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6057..8156)
                     /locus_tag="ACHBFA_001050"
     CDS             complement(6057..8156)
                     /locus_tag="ACHBFA_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM9394559.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8376..9485)
                     /gene="rpoD"
                     /locus_tag="ACHBFA_001051"
     CDS             complement(8376..9485)
                     /gene="rpoD"
                     /locus_tag="ACHBFA_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9394560.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9509..11350)
                     /gene="dnaG"
                     /locus_tag="ACHBFA_001052"
     CDS             complement(9509..11350)
                     /gene="dnaG"
                     /locus_tag="ACHBFA_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9394561.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11532..13847)
                     /locus_tag="ACHBFA_001053"
     CDS             complement(11532..13847)
                     /locus_tag="ACHBFA_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9394562.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14032..14892)
                     /locus_tag="ACHBFA_001054"
     CDS             complement(14032..14892)
                     /locus_tag="ACHBFA_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9394563.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14951..15583)
                     /locus_tag="ACHBFA_001055"
     CDS             complement(14951..15583)
                     /locus_tag="ACHBFA_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM9394564.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            15750..16511
                     /locus_tag="ACHBFA_001056"
     CDS             15750..16511
                     /locus_tag="ACHBFA_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9394565.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16537..19128
                     /gene="mprF"
                     /locus_tag="ACHBFA_001057"
     CDS             16537..19128
                     /gene="mprF"
                     /locus_tag="ACHBFA_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9394566.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19316..20503)
                     /gene="tuf"
                     /locus_tag="ACHBFA_001058"
     CDS             complement(19316..20503)
                     /gene="tuf"
                     /locus_tag="ACHBFA_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9394567.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20871..21227
                     /locus_tag="ACHBFA_001059"
     CDS             20871..21227
                     /locus_tag="ACHBFA_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9394568.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5834 a         4469 c         3731 g         7259 t
ORIGIN      
        1 attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg atctttttga
       61 tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac ttaaccatgg
      121 aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt tgatcaagtt
      181 gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca tctgtgttgc
      241 gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg cgatactcat
      301 aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc ttcctttatg
      361 ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg ttgataagaa
      421 actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca gatgtaatac
      481 tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca tgctgatcaa
      541 taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga tcggaaaagc
      601 aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca ttttctgtac
      661 acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa ctttttgacg
      721 tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag ttatttgatc
      781 aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt atcggaattt
      841 caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg gtcgggaatt
      901 tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca ttatacatat
      961 ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt tgagtggttc
     1021 agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg taggccttgt
     1081 aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc aactgcttga
     1141 agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac gatactgtgg
     1201 tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt tcgtccttcg
     1261 atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc ttttaccttt
     1321 gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc aaaatctcca
     1381 tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac aggatagata
     1441 cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc atctgcaaca
     1501 gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc ttgataaatt
     1561 gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa ttcccttaca
     1621 acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt tcgacgagaa
     1681 gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat tctcctttat
     1741 tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt tccatttctt
     1801 gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag tagccagttg
     1861 ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca atcagtttat
     1921 gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc tcgatttcag
     1981 ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg gcttgagata
     2041 atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct tttttagtcg
     2101 ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag ttttgagtgt
     2161 aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga tttaacagtc
     2221 cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg gcttcaagtt
     2281 cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat tgattcatag
     2341 ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa agccataaaa
     2401 gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt acagccatgc
     2461 gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt tgtgtcatcg
     2521 ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat tttctgcatg
     2581 gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc agcatccgct
     2641 aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc tgctttttct
     2701 gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt gaccaattct
     2761 tctcgaggat cttcttccca taattcttca tcgtctgtga cgaattcttg gacaggaagc
     2821 aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc tcctgcaaca
     2881 gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt gatcgtagcg
     2941 atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag cagatcgaga
     3001 ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac tctttcttct
     3061 ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca cggatggaga
     3121 catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc tcaccgcgat
     3181 aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc cagtaaatac
     3241 caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt tcataatcgt
     3301 cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc agtttcttat
     3361 ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt gttaacatct
     3421 tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc gtcattcttt
     3481 tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct aacaattcct
     3541 gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta tgccgcagcg
     3601 tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc agatttttcc
     3661 agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc atttcagatg
     3721 cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat tggatcgcat
     3781 aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt accagtccaa
     3841 gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc atcccagtag
     3901 catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc gtatcaggtg
     3961 cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc tgtgctttct
     4021 tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc cgtaaaaact
     4081 gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct tttttttctt
     4141 catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc catgaagcaa
     4201 ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag ctttgtcgtg
     4261 tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg atttgctctt
     4321 tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt ctgatagtct
     4381 ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact ggtttctttt
     4441 ttctttttca tagcattcct gacagattcc atggaacgtt agccgatgat cttttactaa
     4501 aaaatgataa cgttcttcta ctacttgctc cacttcaagt aaaagatcct cttctacttc
     4561 ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg aaatgtttgg cgccttcttt
     4621 tctcaaatca taacgggcta atccatcatt aaaacttact ttatcgacaa ttttcaattc
     4681 tgtcaagatt tcaagggttc gataaacagt agcaagtcca atttccggac tctttttttt
     4741 cactaaaaag aagatttctt ctgcagataa atgctctttc tcattttcca aaagcacaag
     4801 cagagtagct tctctttgcg gtgtcagctt aaaccccgct tcatgtagct gctgttttgt
     4861 ttttttcatc gccgaaatag cattcatgac catttactca accccaacaa tttataataa
     4921 ttacaaataa aaaaagatat aacactatgt taattaaaat tattataagc tagttaagaa
     4981 aaacatgcaa gggattctca attagatttt ttgactagtt ttgtcacacc gttttcttgt
     5041 gtaatcaatc cttgtttcaa aagattacct aatgcgcgct taaatgctcc tttgctgata
     5101 ccaaaagctt tcatgatttc ttctggatcc gatttatctg taaagttgat ttgctgatct
     5161 gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat ctgatatagc ttcatatgct
     5221 cttggcttca atgaaagatt cagtacaccg tctggtcgga caccaatcac tcgacctttt
     5281 atctgctcgc ctagacgagg ttcattatat cgttcagaag gatggataaa cccaagatag
     5341 aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc ggtatgctgt tcctatcaca
     5401 tctttgtttt tcatttcttc tgttccatat ttgcttaaag atttgaatat tttttcatct
     5461 gctaaactgc cccaaatacg gtcttttgca tcaactctaa gggaaatcat caagcgatcc
     5521 ccttttttag gccatagctc tgtcattgtc ggcaattcat caagagatac gacaaagtct
     5581 ttatctggaa gaccgatatc gacaaacgca cctaaatctt ttcgtgtagc agtaacgatt
     5641 ccaaaaccgt aatgaccgat acggctttta ggaatttgtg tagtgatccg attttcatgt
     5701 ttttgatttt ggtaaccgaa tccttcaact gcttcaccga ttttgtgttc accttctgat
     5761 ttgcttaagc caaaggtttg tccgtttttt tggacaaagt agtttttttc attttcatca
     5821 ataataatac ctgtaaatat agttgctaat aaatcgttca tttttttctc cctctatagt
     5881 gaaaggtttt cttaatacat ttctttgcta tcataacata atgttatcta cttgaatagc
     5941 tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa cagttgtgtc atactcccct
     6001 ttttcgaata aacggtgtta aacggcaggc tcacagcctc tttcatttgc ctaaaattaa
     6061 ttgctcccat ttcctccgaa ccatccagtt ttacagttga tggttacgat gtaaaatggc
     6121 aaagcacttt ctccaatgcc tttcatcaat gcagattttg ttgaagtggc atatagatta
     6181 taatagcctt ccccattttc gattctggct ttttctaatc tgtaacctcc ttcaacgata
     6241 tatccacgtt ggatacatgt agcaatcact ttgctttgga cacctggtgc ccacgtccat
     6301 gctggattag atgcatcggc aatatcactt gctctggcag atttgattgg ctgattcgct
     6361 gagtttgaag aggaaggggt cgaggatgcc gcttcttgct gtgctgcttg tgcgaccgca
     6421 gcttgttgag cggcttgagc tgcagctgct gcttgagcgg cttgttttgc ttgttcttca
     6481 gctgcttttt tcgcttcttc tgcttttttc ttttcctctt cttctttttt ctctttatca
     6541 gtgagcatct tatctacttg tttcaattgt tcattcaact gagcagatag atctgtattt
     6601 ttcacttttt ctactgcttc ttttgccgtc ttgtattggt cgcgtgttgc tgaatccact
     6661 acttttccgt ctttaaagac tatagctgtt ttttctttag ctgtttgcaa ttcagcatat
     6721 tgatttttag cttctgtttg tgcttgttga atcaattttc caaaatctgt gttatccgtt
     6781 tcgattgctt ggatctcttt atctgctttc aacttgatgt catctgctag atgatcatcg
     6841 acgatggcag gtgctatgaa ccactcattc actttttgga tatctcggat cttagattgg
     6901 atatcttggt aagttgtttc taactctgaa tatccttttt cattcttaag actggataaa
     6961 tcatttttta gttttgataa atcttgatga atcatatctg cacgaataaa ttgatggtga
     7021 tcatctgtat aaaaggcagc aagtttctca tgaagttctt ccatttgttg ctgtcttttt
     7081 gcttctgctg ctacttcggc ttctactttt tgatgatggt cataataagc ccagcttcct
     7141 ccaccgagta gaagtactgc tgctaaaacg agataaggta ctttttttga ttttttcttt
     7201 tgcgctttat ctggatctgt tttttctaag tttgattctt gatgtgtttt ttcagtatct
     7261 ggcacgattg ttattgattt cgcttctgct tcaaacagct taggctggtt ctcttctttt
     7321 tctatttttg cttcaagatc agtaccctct ttgcttacag attgattttc agcatcggat
     7381 tcactattta tttcatcggg ctctgttgac tttttgtctg tgttttcgag tatttctttt
     7441 ttctctgctt ccggcagttt attcgttttt tccttttcta attgtttttc agtaaatact
     7501 tcagtttttt ctgtgatatc cgtttttacg tcttttattt ttactgtttc tttacctgtg
     7561 gcgttttctc tatcagagtc tttttcagtt tcttgttcct tttctggctc tactttgttt
     7621 tcctgtgcaa ttttttcact gatcattttg agcagctctt tcgcgtgctc ttcttttgta
     7681 tcttcacgat gtgctttgat ataagcggat agtatcgaat tttcttcctc attatcgacg
     7741 atttctttca gttcagctac tcttcttttt tgttttgatt caagcgtttc ttcttgattc
     7801 tttcctgatt cttccgaagt ctcatgaaca gttgctttct tctgttcatt tttctctata
     7861 ttagttactt gatcgtgagc aggtggttct tcgtcttctg gttctgcttt ttcgaataat
     7921 tccattacag attctagagg taaatctttc aattccgacc attcgattgg atcgtttaat
     7981 tctgtatcag gaaagactgc atcttcagtt gatttatcct gcatagctgc atctgtattt
     8041 gtttcttcaa ttgattcttt tatttgctga tcttctgcag ttatttgatg accgcagtct
     8101 ggacagatag tattctgctc catttttttc gatccgcaat ttggacattt tttcatcact
     8161 tagcgatcgg ctcctttact aggtttacgc taccatttaa tctatcatat tctatacgtc
     8221 acgccaatag cttattttta ggatttactt aaaaataatg aatgaatttc cttcctatag
     8281 aaaaaaaagc ttgagactgc gtagtctcaa gctttttttc ttttaaaata aaccaaacag
     8341 ttttactgat ttctcttctg gtcaatagtc cacgcttatt ctaagaaatc ttttaattgt
     8401 tttgaacgag aaggatggcg caattttctt aatgctttag cctcgatctg acgaatacgt
     8461 tcccgagtaa caccaaacac tttccctact tcttccaatg ttctcgtccg gccatcatct
     8521 aaaccaaaac gtaagcgaag gacattctct tcccgatcag tcaatgtatc tagtacatct
     8581 tccaattgtt cttttagtaa ctcataagct gcgtgttctg ctggacttgt tgcttcctgg
     8641 tcttctatga aatcacctaa gtgagagtca tcttcttcac cgattggcgt ttcaagggaa
     8701 acaggttctt gcgcgatttt caagatttct cggacttttt ctgttggaag atccatttcc
     8761 gcaccgattt cttctggcgt aggttctctt ccaaggtctt gcagcaattg acgttggata
     8821 cgtatcaatt tattaattgt ttcaaccata tgaactggaa tacggatcgt acgtgcttgg
     8881 tcagcgattg ctcgagtgat ggcttgacga atccaccatg tagcataggt tgaaaactta
     8941 aatcctttgc ggtagtcaaa tttttcaact gccttcatca gtcccatgtt tccttcttgg
     9001 atcaagtcaa ggaactgcat gcctcgtcct acataacgtt tagcgatact tactactaaa
     9061 cgaaggtttg cttctgccag acgctgtttc gcttcttggt cgccttcttc gattttcaat
     9121 gctaacgcta cttcttcttc cgcagttaaa agggagacac ggccaatttc tttcagatac
     9181 atacgaaccg ggtcattgat ttttacaccg gttggcgcag aaagatcttc tgtttttgct
     9241 tgttcagctt ttttctcagc actcttcaag ctgtgaattg atggatcacc attttcatcc
     9301 accacactga tacctgcgtc ttcaatcttt tggatcaatt tgtccatttc atctgcattc
     9361 aaagtgaatg gtgtagcaag ctgattggat aaatcatcat atacaacttg tcgtttgggt
     9421 ttgttttctt tgatgaaagc tgctactgct gcttcatacg tttttttatc tgttccattt
     9481 gccataaaag aaggcccccc ttgagtagct atttggcttg ttttaactgt ttcgttaaat
     9541 tgatgatttc aatcgtcaat tcaagctcaa gctgtttgtt tccagttcgt ctggcttctt
     9601 gctgcatttg tttttttaat gttatttcat cagctaagcc agatttgctg atcacatcta
     9661 gcaagtcttg gatttctcgt ggcgaacttt cttctgccac cggtaaatat tcgatttcca
     9721 ccaccaaccg ccttaaattt tcatcttgca aaaagtctaa aaactctgca aggttgaatt
     9781 cataatgttg tgagacatat gcatcgaata aaaaataaac ttctgcatat gcatcatgaa
     9841 taaactgaaa ttctcgttct tttaaaatat tacggacaga aacttcttta aatgcacgat
     9901 ataatagaat ctgctctgcc ttttctactt gcgtcaaagg tctctttttc cgtgcagttt
     9961 cttgtctcgt aacctgagtg tgttgacttt cctgaggttc ttgacgattt tgctgatttg
    10021 tccttcgaag atcgcgcagt tgtttttgta tcgtctctct gccgatctga aactctgttg
    10081 acagttgaga tagatacata tcttgttcga ttggtgaaac aactcggcta agttcaacta
    10141 gtaattcttc gaggtattcg atcttgtctt tttcattatc taaatttttc ccttgtttta
    10201 aataacgtga tttaaaagta aagactgttt cgcgcccatg agccactaat tcttggaaag
    10261 atgcttctcc atacttacgg agatattcat caggatcaag cttttctgga acgactacga
    10321 cagaaagtgc taatcgactg ttttgccgaa gcaattcaat ccctcgattg gttgcttcaa
    10381 aacctgcgtt gtctccgtca tagcaaaaga ccaattcttt cgtcagcttt tccatttgtt
    10441 ggatctgttg agctgttaga cttgtcccca tagaagctaa tccagttttg ataccagcct
    10501 gccatgcagc aatcacatcc ataaaccctt cgaacaacaa aatttctccg tttttccgga
    10561 tatcgcttct tgctttatca agattaaata aaacttgtct tttattgaac agttctgttt
    10621 ctggactatt caagtatttg ggctgatctt tttcctgccc tttttcaggt gcaagccagc
    10681 gtcctgaaaa accgatcgtc tggccttgag aattacgaag tggaaacatc actctttgat
    10741 aaaaacgatc agacatacgg ccatcatcat gcgtgacaaa caatccagag cgttctaaca
    10801 attcatttga aattgattca ttcttgaata cttgctgaag aaaagaacgc tcattaggtg
    10861 caaaacctat gttgaattct tcaattaatt ctaaagacaa cccacgactt tgcaaatatt
    10921 ctaaagctgg ctgtccagct tttgtatgca atagcatgtg atgatagatt tccgctgctt
    10981 tttcatgagc agcaatcagt ttccccgtag cagattcagg attttggaca gctatagtac
    11041 gctgacgcca ttgatcttct aacggtatct gttcgaagtc tgccactttt accactgctt
    11101 ctggaaaact tagaccttct agttcttgaa gaaaagtaaa cacgtttccg cctttaccac
    11161 aaccaaagca atgaaaaatc tgcttgtctt ctgcaacaga aaaagacggt gtacgttctt
    11221 catgaaaagg acacagcccc aagtaatttt taccagactt tttcaactgg acatattgtc
    11281 caatcacctc aacgatattc gtttgactgc gtactgtttc gataacctct tgaggaatcc
    11341 gttgagccat tttttcaccc cctgctctat tgatatcttt tgggtttaac agaagatatt
    11401 tcaggagaat aaccggtaaa aaatcgcact aactgaaatc agtgcgattt taaaggacac
    11461 aattatacat gtttgattgt atcatatttc attgcttttt caagtataac acatttctat
    11521 ctcattaagc atcaatttgc tactcttttc agtaactgat tttgaatatc agccttccag
    11581 tacaatactc gaagaacaat atttacgagc atgaatacaa agaagtatcc tacgctctct
    11641 cccaacccta agtttacttc caaaattcca tggaacagca ttgcagctac atatatccct
    11701 acgatcgtcg ttactgctag gaagatgcgc aatgggttga agggataaca agcttttaca
    11761 actgccatgc aactgatccc aatcagcaag taatacatca aagtagtagt ttcagcattt
    11821 ccccatgcga atgtttttcc aagtagatac accgcaatga tattcactac tacaagcaag
    11881 gcatttggta aagcattaat aagtgctgta ggcaaataac ggtaagtgat ttttctttta
    11941 tcttgttcaa aagataaaaa gaaggaagga tacccttcaa tcgctaaatc aatcagcgtg
    12001 atttggatag ggataaacgg aaatccaatt gctgtaacgg cacatataat cgataaaata
    12061 aatgaataaa tcgttttgat aaaaaagatg cctgagactt tcgtcacatt gttgaccact
    12121 cggcgtcctt caaacaggac ttctggcaat gtagtaaaat cagagtccaa caataccaga
    12181 ttcgcaatct gacgtgttgc tccatcacct tctgccatag caatgctgca atctgcttct
    12241 cttaacgcca aaacatcatt cacaccgtcg cctgtcattg caaccgttct gccgctttct
    12301 ttcaattcat ttaccagcaa tttcttttgc tgcggactca ctcgtccaaa aaccgtatag
    12361 cgatgagcag cttctcgcac ttccatttct tcagtaattt gagaaagatc gacatacgca
    12421 tcataattcg gtaatcctgc acgtctagcg atattggaga cagtaaaagg attgtctcca
    12481 gaaatcactt tcagatccac accttcctca tgaagataag ctagtgtttc ttgagcgttt
    12541 tttcggattg gatcatcgat ttcaaacaga gcaaccggct caacaaatgc tggtttttct
    12601 tcgttcaatt gctgatttgg agcgatcccc aacataagta cgcgatagcc attcgcttgg
    12661 gctgttttta gactgtccgg aagccgatct tcttccacca aacgttcagg cgctcctacg
    12721 tacacgactc ccagttctgg aaattccatc gcgccccatt tgcgatcaga agaaaatgcg
    12781 actttgtttg tcacatcata ccgattagaa agctcaaaat aatctctcat agcccgcata
    12841 gtgatgttgt tatcactgct ttctgccaga tacgtcccca taatttctgg gatggctttc
    12901 tcgtacagcg gatgtagtgc ttctacccgc tggatactca ttttcccttc agtaatggtc
    12961 cctgttttat ccaagcataa tgtatcgaca tgggccagcg tttcaattga atacatgtct
    13021 tgaacaagga ttcgtttttt tgccagtttg gttacccctg tcgttaatgc aatacttatt
    13081 agcaatacca aaccctttgg aagcatgcct aacaaagctg ctgcagatgc aataacagaa
    13141 attttgatag aagcatcgcg gatgaacagt gcttccaaaa atagtatgat ccccaaagga
    13201 ataatcacga aactagtgaa tttggatact ttgcgaatcg aatctacaag ttcagaacga
    13261 atagctttgt gagtctttgc ctcgctagtc aagcggacag cataattatc tgcacctact
    13321 cggataactt cagccatgac agaaccgctt gtcaaaaagc ttccagaaag caattcagct
    13381 ccagtttctt tgacaatcaa atcagattcg cctgtcaaaa gagcttcatt tgcttctgcc
    13441 cgtccagata taacctgtaa atcggctggt atctgttctc ctgctccgat ttttaccaaa
    13501 tctcctaata ccagctcttt agtagaaatc gattgttctt taccgtcccg gatcactagt
    13561 acttctgatt gggaaacaat cgataatttc cgtacaagat ttcttgcatg tatttcttga
    13621 taaattccaa taacgatatt gacgacaata attgctaaat acgccatgtt ggaatacgca
    13681 cctacaaata acagacaaat cccaatacca aagttcagaa aattgaataa cgtcaatata
    13741 ttatcgctga atatttgttt tgtacttttt gatgcatctt cctggtagtc attttgctgg
    13801 ccagaagcca ttttctgaac tacctcttgc tgtgtaagtc cattcatttt ctttttctcc
    13861 attttccgcc aactttcaaa aataatcacg tgtttaagtc tagcgtgttc caagattaaa
    13921 aacaacttgt ttttataaaa tttatagaaa aacacacgaa tgattttaca aaaaagatgc
    13981 caaccttttt gtttgaatca cccgtgtgct gtttttatta tcaatctatc tttacttata
    14041 ttgttcctcc aaacggctca tccaccaacc tagtgctgta ccggctataa aaagaacagg
    14101 acaagctagt accccagcaa ctcccaatcc cgcgtaatcg gtaggaatga tcataatcgt
    14161 tgaagcaaaa actacaccta aaatgaaatg gaatagcttg ctatatgcct ttgagaaaat
    14221 gtaatccatg atttttgaca atccaagaac acaaagaatc ccaccaattc cgatcggcac
    14281 aatcacttca agtcgtactt ctttaaatcc gtcagccatc gctttataca atcccatata
    14341 caccaaaaaa ttagaagggc tcaatcctgg gacgatcata cccaaaccaa tcaatccgcc
    14401 ggcaatcagc catgttccaa aattctgatc gacttgcccg tcaaataatt gagcgccgta
    14461 taataaaaac aaataagcca gaacaaaagt aacaaccatg atgatcacat cagaagtatc
    14521 tcttcctttt ttccctgctt ctttccataa cgcaggaaca gttccagcga tacatccgac
    14581 aaaaaaccat aatatcgttg tttcatacgc acctaataaa aaactgacta aaaaagaaag
    14641 tagaaagatg cctgtcactc cgcctagacc aacaggcaga aaaaacaaaa cattttcttt
    14701 gaaatttttt gtaaggtggg ctaaaaaaga aatcagccgc tcatacatgc caaagacagc
    14761 tgctaatgca ccaccgctga ctcctggcaa gataaatcct gaaccgataa acattccctt
    14821 cacaaaccgt aacatccagt ctttggttgt gtaccccact tcttgatttt ccattttttg
    14881 ccttttttcc actatttgcg cactccttct ttcatttaat tttattcatg attgaacatt
    14941 tcctgctatt ttacttaaag actttttcat aggtctctat tattttttca ttcattttga
    15001 caatatcagg ataaaacatg ttacctccat tatgatagag atagccgcca ttgtaaagca
    15061 aggactggat ccgccaatat cggtattgtt tcccttcctc atttcctaaa agtgggctca
    15121 gcacatcttt agaatacgct tctgctaatt ctactgtgtt ttctccgcca tgatttttta
    15181 cataggcaat ataatctttc ccaaaattat atgcttggac agctgtccat aaatcgcatc
    15241 cttgtttctt tgcttctact atcatttccg ccaaatattt gacaccttga tcaatactgt
    15301 cagctggatt atcaatgatc tcagcttctc catgcagact ttccgagctc tgcatgagat
    15361 ctgtcccttt tccttttgat tcggtcaata ttatcgcctt gatcaaattc tcgtagtttt
    15421 ccatatgata attttttgca gctgttgtta tttctatttg atagctttcc actcgtttta
    15481 ccgaacgata agttcttcct cctaaaaaca acaccgcacc aataaccgtc agaatgatca
    15541 tgtaaaaaag aatacggata atagataatc gttttcgctt catttcatca cctagttatt
    15601 ttgacatctt tttttatctt actatttctt tatcttcttg gctatttctt ccttgatttc
    15661 tgtgtacgcc ttaagaaaaa atgaattttc ctgtattttt tgacatctta cgaaaatgat
    15721 cgttgttttt cagtaaaaac aattgcccaa tgactgatat aatcgttata ctttacacag
    15781 taggtgatta tgtgaaaaaa gaaaagaaat atgcaaaaat gccagatggg agcgaaattt
    15841 actatgaaaa gagcggtcaa ggcttccctc tctttttact gcatggcaat gacggcagcg
    15901 gccgcttttt ttcagaacag gtccctgtac tcgaacgcta ctataccgtg tatctagtag
    15961 atagccgagg acatggtcgt tcgacaaacg aagcatctat gttgaatttt cagttaatgg
    16021 ccgaggatct gaatacgatc atgttgctgg aaaaaatcga tcaggcagat tttcttggat
    16081 ttagtgatgg agcaaatcta gcactggtat ttgccagcag cttccctaaa aaagttcatc
    16141 gattgatttt aaattctgga aacacgcttg ttaaaggcgt tcgtttttct gccagagtca
    16201 tcagtaatat tcattatgca tgggtatggt tactctcttt atttcgccca agtcttcgca
    16261 aaaatctttt agttatcaaa cttttgctgc atgacatcgg gcttacagaa aatgatttaa
    16321 aaaaaatcaa ctcccccact ttgatcatcg tgggaaaaaa agatgtaatc aagctaaaac
    16381 attctttgta tatagctaaa actataccaa aagcttcctt tgtgcttgtg aaagaacaag
    16441 gacatgaatt agctagaaaa gatcctgaac gcttcaatcg agaagttctt caatttcttt
    16501 ctgaaacata actattagga atagaaggtg atttatttgt taaaaaatac catacaatgg
    16561 ctgaaaaacc acctaggact gttcaaaaca atctttttga tttctgtcat tgtcattatt
    16621 gtcggtgaat tgatgtccat cggtaaaact ctatctatcc agcaattagg agaaacgttt
    16681 gccactatac ctgtatggaa aagcggacta atgctattga ttggtcttat ctctgtgtta
    16741 cctatggttg gttatgatat tattttgaat cggatgcttg aacaaaagca gaatcctcgt
    16801 tacctgtttg agacaagctg gctgatcaat acgatcaaca atattgccgg tttcggcggg
    16861 tttgttagtg tggggcttcg ctctgaacta tatggacaaa agaaagagag cagaaacgtg
    16921 atccaagcat tatcgaaagt tttcttgttt ttgatggctg gactttctat ttacagtctg
    16981 ctttcttttt tacttgtagt cttcacgcca gttgctcctt ttttgaagca atattggata
    17041 tggttgatcg gcggcggtct atattttcct gtcgtctttt tatttactac tttcaaaaag
    17101 aaaggattcc ttggtggtac ctcacttaaa actcaaggac agctattgct agtttcattg
    17161 ttggaatgga ccggtgtcct gtttagtttc ctttctgttg gttatttaat ggaaatccgg
    17221 attgatctgt ggcaaacaat tccattattc attgctgctt cagtgatagg aatcgtgtct
    17281 atgattccag gagaaattgg gagtttcgat gtaatgatga ttatcgggtt gtccgctatt
    17341 ggtgtgccaa gagaaatagt cgttgtgtgg atcttgctct atcgcctgtt ctactacatc
    17401 attccgtttt tgattggaat cgtctttttc ttcaaaaata tcggttccac ttttgatcag
    17461 cgttattctg gtatccctaa acaattagca acagaaattg cgcataaaat cgtggttgtc
    17521 ttattatact tttctggaat catgctagta ctttctgcca ctatcccgca agcttttaca
    17581 gagtttcgct ggcttcatag tttgaaccca ttgaaatttc attttattat ccaatttcct
    17641 agtatactat tgggatttct tttaatcatt atgggcagag ggatcgcagc acgtgtgaaa
    17701 cgagcatatt tgcctacaat cttcttgatt gttctagctt tactttatgt tttattaagt
    17761 gattttagtt tcacaccggt ggttttcttg tctattctgc ttctgatcat tcttgcttct
    17821 aaaaacgaat tatttcggga acaattgatc tattcatggg agtggcgtac gatcgatgga
    17881 atcattattg gtgcactgtc cttgctttac atcgttattg gggtatacaa tctgccagat
    17941 ttccctcatc ggcgtcacca ttttatttcc tttttcttat ttccttctga aaagatttgg
    18001 ttttcgggat tgttggccat catcgctgtt agtttcatga tcgttctatt tgttcatttc
    18061 cttcaaggag aaaagaaaca gattggcgaa gcctttaatg aagaaaaggc cctgaagatc
    18121 cttacaactt atggaggaaa ctccgatagt caattgatct tcttaaaaga taagcggatg
    18181 tttgcctatg aaaaagacgg tgaaccaact gttcttctcc aattcgcttg cttcaataat
    18241 aaatgtattg taatgggtga tccttctggt aagaaagaag attttccaga agcaatcgaa
    18301 gcttttatcg aagaaacaga tcgactatgc tatttaccag ttttctacga aacaagcgaa
    18361 gagattgtta tgattttgca tgaattcggc tacgatttca ttaagatggg ggaagaagct
    18421 tatgtagacc tgaatagttt tacaacttca gggaaaaaaa tgaagggcac tcgtgctgtc
    18481 ttgaatcgga tcgaacgaga aggatttaca tttgatgtct tacaaccacc attttctact
    18541 gagcaaatga gtactttcaa aaatatctct gataattggt tgggttcacg aaaggaaaaa
    18601 gggttttctt taggcttttt ctctgaagat tatctacaaa gagcgcctat tgcagttgtc
    18661 aaaaataccc aagaagaaat tgtcgctttt gcaacgatca tgcctactta cacgaacaat
    18721 aaagtaggaa cgattgatct gatgcgatat gattctaaaa aagcaccttc tggcagtatg
    18781 gatttcttgt tcttgcattt gtttgcctat atgaaagaag aacatatcca atggttcaat
    18841 ttagggatgg cccctctttc aaatgtcgga acttccagaa agagtttttt acaggaaagg
    18901 attgcatact tggtttacga gtttggttct catttttatt ctttccatgg attaagagaa
    18961 tataaaagca agtacgctac aaactgggtc tcacgttata ctctatattc tagagatagt
    19021 tggattgcat atgttatgat tgctttgctg attattgata atgcgccagt tgagcagaca
    19081 caaggtagaa taagtggttt gaaaaaatgg atacgaagaa agtattaaaa actgcatacc
    19141 gatagatgaa cactctcatg atgaataaaa gagactgaag taaaacgaaa gttcatgctt
    19201 ctacaatctc ttcttataca attgaagaac aaagagcggt ccaaacagaa atcctgtttg
    19261 gaccgctctt ttattttaaa catttttcat taccaaaata ttcgagtcgc gtttcttatg
    19321 cttcgatctc tgtaacgata cctgaaccta ctgtccgtcc gccttcacga attgagaatg
    19381 tcgttccgtt ttcaacagcg attggatgga tcaagtcaac atcaatcgtc acgttgtcac
    19441 caggcatgac catttctgta tcttctggca atgtgattgt tcctgtaaca tcagttgtgc
    19501 ggaagtagaa ctgtggacga tagttgttga agaatggagt atgacgtcca ccttcttctt
    19561 ttgtcaacac gtacacttct gctttgaatt ttgtatgtgg agtaatggaa cctggtttag
    19621 caagtacttg tccacgttcg atatcgtcac gttggatccc ccgtaacaac acgcctacgt
    19681 tatccccagc ttccccgtaa tctaacgttt tacggaacat ttctacacct gttacaactg
    19741 ctttttgtgt ttcaggtttg atccctacga tctctacctc atcaccgaca cgaacagcac
    19801 cacgatcaat acgacctgag gcaacagttc ctcgacctgt aattgagaag acgtcttcca
    19861 ctggcaatag taatggttta tctgtatcac gttctggtgt tgggatatat tcatctactg
    19921 tatccatcaa ttccataata gcagcttcag catctggatc gccttgcaat gcttttaatg
    19981 cggaaccttt gatcacagga gtgtcatcgc ctgggaaacc atattcgctc aataactcgc
    20041 gaacttccat ttctaccaaa tcgatcaatt cttcatcatc gactaaatca actttgttca
    20101 agaaaacaat caggtatttt acaccaactt ggcgtgacaa taaaatgtgt tcacgtgttt
    20161 gaggcatcgg accgtcagtt gcagaaacaa ctaagattgc accatccatt tgtgctgccc
    20221 ctgtaatcat gtttttcaca tagtccgcgt gtcctggtgc atcaatatgc gcgtagtggc
    20281 gtttttctgt ttcatattct acgtgtgcgg tattgatcgt aatcccacgt tcacgttctt
    20341 ctggagctgc atcaatacta gcatagtctt gaggatttgc taggcctttt ttgcctaata
    20401 ctgtagtgat cgcagcagtt aaagtagttt tcccatggtc aacgtgtcca atcgtaccaa
    20461 tgttaacgtg tggtttactt ctgtcataat gttcttttgc cataatttac aaacctccta
    20521 aataatatag atcactgaaa aatttcagct ctcacctttt tattatagtc tttttttgac
    20581 taaatcaaaa agaatacgtc cgacttactt tttgtaagat tcgaactaag cttattatac
    20641 gtcaatattg gtcaaagtca actaagagca ctcgtctgtc ttatacgcga aaaaagtatc
    20701 ttttataaat aaaaaaatgt gttttttgag aaaaacttca agatagtcct taactttctt
    20761 ttgaagcaat aagaacaaaa tgaggaaaag tttaaaattc agctgttgcc aacaactttc
    20821 aatagacgaa atcttctatt tcgttaataa taaggaaaga ggtgaagtac atgaaaaaat
    20881 taattggcgt acttgtcgtt ttaggcatat ttctcggcgg aagttttgca gcttatcatt
    20941 atttttacgg cggcgaagct tattatacaa aaatcacgac gaatggcgaa atgtcagcta
    21001 cccaagcaga taacggagag aaattcacga cttataccta caagcaagcg gcttataata
    21061 aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag agaacatcca ttaaagatga
    21121 atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt attaagctgg gaagaaataa
    21181 aggcttctga agtgcctaaa aatgcggcag aaaaaatcga taattagtaa atggtcttaa
    21241 aattttatta gataaaaaga ggctgggaca gaagcgttta actccaagaa ata
//
LOCUS       DAYYNG010000035        20993 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000035 DAYYNG010000000
VERSION     DAYYNG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.035"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            189..3467
                     /locus_tag="ACHBFA_001060"
     CDS             189..3467
                     /locus_tag="ACHBFA_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM9394569.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4008..5915)
                     /locus_tag="ACHBFA_001061"
     CDS             complement(4008..5915)
                     /locus_tag="ACHBFA_001061"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM9394570.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6045..6920
                     /locus_tag="ACHBFA_001062"
     CDS             6045..6920
                     /locus_tag="ACHBFA_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM9394571.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7000..7974
                     /locus_tag="ACHBFA_001063"
     CDS             7000..7974
                     /locus_tag="ACHBFA_001063"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9394572.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8154..9299
                     /locus_tag="ACHBFA_001064"
     CDS             8154..9299
                     /locus_tag="ACHBFA_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9394573.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9436..9780
                     /locus_tag="ACHBFA_001065"
     CDS             9436..9780
                     /locus_tag="ACHBFA_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9394574.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10051..11337
                     /gene="efmA"
                     /locus_tag="ACHBFA_001066"
     CDS             10051..11337
                     /gene="efmA"
                     /locus_tag="ACHBFA_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM9394575.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11475..12074)
                     /locus_tag="ACHBFA_001067"
     CDS             complement(11475..12074)
                     /locus_tag="ACHBFA_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9394576.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12478..13599
                     /gene="mnmA"
                     /locus_tag="ACHBFA_001068"
     CDS             12478..13599
                     /gene="mnmA"
                     /locus_tag="ACHBFA_001068"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9394577.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13845..15266
                     /gene="arcD"
                     /locus_tag="ACHBFA_001069"
     CDS             13845..15266
                     /gene="arcD"
                     /locus_tag="ACHBFA_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM9394578.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15280..16398
                     /locus_tag="ACHBFA_001070"
     CDS             15280..16398
                     /locus_tag="ACHBFA_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9394579.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16458..17750)
                     /locus_tag="ACHBFA_001071"
     CDS             complement(16458..17750)
                     /locus_tag="ACHBFA_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9394580.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17900..18460
                     /locus_tag="ACHBFA_001072"
     CDS             17900..18460
                     /locus_tag="ACHBFA_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9394581.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18589..18966
                     /locus_tag="ACHBFA_001073"
     CDS             18589..18966
                     /locus_tag="ACHBFA_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9394582.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18899..20971
                     /locus_tag="ACHBFA_001074"
     CDS             18899..20971
                     /locus_tag="ACHBFA_001074"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9394583.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6801 a         3803 c         4469 g         5920 t
ORIGIN      
        1 ttttatacgt tattttaaaa aaacttattc tttttccgtt aataaatctc tttattatca
       61 acaacttaag ttatattttt tctttttata aaatacactt attataattt tgttttttat
      121 tgacagtaaa aataaattag gtaatacttt aagcaaacgc tttctaattt gttacgggag
      181 gaatacactt gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc
      241 tcagtacccc ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga
      301 acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa
      361 ctagtgaaga tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg
      421 aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag
      481 aaagcacaag tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt
      541 ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc
      601 aagcaatccc caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga
      661 cctatacgaa tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc
      721 atttgtttgg gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat
      781 atgtctggga ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc
      841 gacttggatt ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc
      901 cagctcctgg gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg
      961 aaaaagaaac aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca
     1021 cgaactcgat tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat
     1081 attattacga tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa
     1141 aattatacgc ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga
     1201 cgatcaaggt agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc
     1261 aagaaagacc tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag
     1321 gaaaccaaga tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa
     1381 ataaagcaac aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg
     1441 aatttgatta tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc
     1501 cagataaaaa ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga
     1561 ttcttcatac agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag
     1621 ggtcagatgc aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat
     1681 acgcatatgc tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat
     1741 tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga
     1801 aaaatccaga aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac
     1861 tgttcagtca ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa
     1921 aaggattaca gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg
     1981 gaaatacagg aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc
     2041 ataacttagt caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg
     2101 atggatttgc ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt
     2161 atcataatgc agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa
     2221 aagtaatcat caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag
     2281 aaggtaacgg taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga
     2341 tcaatagttt gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata
     2401 ttatcgtcaa tagcagtaca agttttaata acggttcata taatgtggct ctttatacct
     2461 cttcagcagg aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc
     2521 ttgagatgag agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt
     2581 tgaactacta ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat
     2641 caaaagattg gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc
     2701 gctatgaaaa tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc
     2761 aaacagaaaa ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag
     2821 aaacctcacc gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa
     2881 aaccaaatga acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg
     2941 cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa
     3001 atcaaggaac gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg
     3061 aaacacagtc aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag
     3121 gagaatatac gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg
     3181 ctcatacaga atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc
     3241 ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac
     3301 caaatggaaa accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc
     3361 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3421 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3481 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3541 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3601 gttcttatta cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat
     3661 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3721 ttttctctaa tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa
     3781 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     3841 aatttttctt actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc
     3901 agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc
     3961 caaattcctt cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc
     4021 actctttttt ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag
     4081 aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg
     4141 atttgcagta aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa
     4201 ttgacaaaga attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag
     4261 cttagaccgc ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg
     4321 tcagcaaaag gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt
     4381 gtaagatctt cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg
     4441 ttattatcaa atgcatggga aatctcatga gcgataacag ctccgattcc accataatta
     4501 gcagagcttg attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg
     4561 atcacattcc gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct
     4621 gaacgatcca ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa
     4681 acaagagtat ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg
     4741 aataatgctg ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg
     4801 acggcttttt cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa
     4861 acggcgatca tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat
     4921 ttttttccgt agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct
     4981 gcttttttct gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg
     5041 acttggcgga attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc
     5101 cagcttttga ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac
     5161 actggatcag ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt
     5221 ttcaagtcca gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt
     5281 ttgctgtaat ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat
     5341 tgcactgctt gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat
     5401 aactgaacag ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat
     5461 gcataatacg tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt
     5521 tgcgcattct tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac
     5581 agccattctg gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg
     5641 attataggtt gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt
     5701 tgataatatg caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc
     5761 agatcgtctg tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg
     5821 cctgtagctg gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct
     5881 tcatagaaat cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc
     5941 gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat
     6001 tcgaacaatc aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc
     6061 gatcccaact gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta
     6121 aaaaattagc cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc
     6181 tggtttttga agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt
     6241 ctccagcaaa atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg
     6301 aagagccaat cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg
     6361 aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt
     6421 ttttgaggta taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag
     6481 cagttctcct tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc
     6541 gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac
     6601 cactaggact tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga
     6661 agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa
     6721 aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat
     6781 atggcaatca ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt
     6841 acaaagtaca cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg
     6901 gaacagtttc acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt
     6961 taaactatcg aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat
     7021 gacacgttga agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa
     7081 gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc
     7141 atcaatatcg aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca
     7201 cctgtcaatg attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt
     7261 gcgaaaacga tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg
     7321 aaacctcatg aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca
     7381 actcgagtac tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct
     7441 gtagataatt tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg
     7501 ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt
     7561 tcagaatatt tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag
     7621 ggtggttatg tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg
     7681 aacacaggag caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa
     7741 gtatatgcct gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat
     7801 gcaccaatca aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca
     7861 gaaaacttaa caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat
     7921 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
     7981 aaaagaggga aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt
     8041 tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct
     8101 ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg
     8161 aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg
     8221 aagcgatgac ggcaatcatg caagatacgt ttggaaatcc ttcaagcata catggatttg
     8281 gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag
     8341 caaaacctca cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct
     8401 tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac
     8461 atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc
     8521 tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag
     8581 atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca
     8641 aagaaatcgg tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag
     8701 cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg
     8761 ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata
     8821 tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt
     8881 tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag
     8941 cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg
     9001 aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc
     9061 gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta
     9121 tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa
     9181 tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa
     9241 ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac
     9301 tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac
     9361 ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt
     9421 atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc
     9481 tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca
     9541 aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga
     9601 ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca
     9661 gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat
     9721 aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga
     9781 tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg
     9841 taaggacgac gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt
     9901 taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt
     9961 tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta
    10021 tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg
    10081 aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt
    10141 atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt
    10201 ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga
    10261 ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg
    10321 attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta
    10381 gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag
    10441 tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga
    10501 atggtccaat cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc
    10561 gttcctgtca attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta
    10621 ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt
    10681 tttaaagatg caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata
    10741 acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg
    10801 atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac
    10861 gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga
    10921 atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg
    10981 gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt
    11041 gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat
    11101 gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga
    11161 ttattatttg caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc
    11221 ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat
    11281 gataaagaat tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat
    11341 ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg
    11401 gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat
    11461 atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt
    11521 tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt
    11581 gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca
    11641 tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat
    11701 tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg
    11761 tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc
    11821 cctttgatca agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt
    11881 gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct
    11941 tttattactg cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag
    12001 agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg
    12061 ataaagtaat tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat
    12121 ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg
    12181 ataagaagca attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga
    12241 aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg
    12301 attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac
    12361 gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa
    12421 agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg
    12481 acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta
    12541 acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg
    12601 gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa
    12661 gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat
    12721 cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg
    12781 atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc
    12841 gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac
    12901 atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca attatcacaa
    12961 gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca
    13021 attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt
    13081 attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat
    13141 atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt
    13201 ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt
    13261 gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca
    13321 ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca
    13381 aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt
    13441 cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact
    13501 cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac
    13561 cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa
    13621 cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa
    13681 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
    13741 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
    13801 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
    13861 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
    13921 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
    13981 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
    14041 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
    14101 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
    14161 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
    14221 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
    14281 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
    14341 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
    14401 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
    14461 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
    14521 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
    14581 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
    14641 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
    14701 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
    14761 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
    14821 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
    14881 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
    14941 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
    15001 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
    15061 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
    15121 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
    15181 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
    15241 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
    15301 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
    15361 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
    15421 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
    15481 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
    15541 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
    15601 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
    15661 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
    15721 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
    15781 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
    15841 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
    15901 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
    15961 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
    16021 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
    16081 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
    16141 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
    16201 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
    16261 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
    16321 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
    16381 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
    16441 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
    16501 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
    16561 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat
    16621 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
    16681 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
    16741 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
    16801 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
    16861 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
    16921 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
    16981 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
    17041 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
    17101 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
    17161 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
    17221 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
    17281 gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca
    17341 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
    17401 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
    17461 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
    17521 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
    17581 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
    17641 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
    17701 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
    17761 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
    17821 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
    17881 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
    17941 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
    18001 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
    18061 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
    18121 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
    18181 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
    18241 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
    18301 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
    18361 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
    18421 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
    18481 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
    18541 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
    18601 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
    18661 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
    18721 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
    18781 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
    18841 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
    18901 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
    18961 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
    19021 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
    19081 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
    19141 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
    19201 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
    19261 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
    19321 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
    19381 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
    19441 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
    19501 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
    19561 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
    19621 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
    19681 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
    19741 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
    19801 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
    19861 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
    19921 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
    19981 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
    20041 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
    20101 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
    20161 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
    20221 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
    20281 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
    20341 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
    20401 aggactgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
    20461 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
    20521 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
    20581 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
    20641 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
    20701 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
    20761 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
    20821 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
    20881 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
    20941 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tga
//
LOCUS       DAYYNG010000036        20870 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000036 DAYYNG010000000
VERSION     DAYYNG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.036"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1898)
                     /locus_tag="ACHBFA_001075"
     CDS             complement(<1..1898)
                     /locus_tag="ACHBFA_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9394584.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGY"
     gene            complement(1911..2186)
                     /locus_tag="ACHBFA_001076"
     CDS             complement(1911..2186)
                     /locus_tag="ACHBFA_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394585.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2548..3003)
                     /locus_tag="ACHBFA_001077"
     CDS             complement(2548..3003)
                     /locus_tag="ACHBFA_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394586.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3157..4365)
                     /locus_tag="ACHBFA_001078"
     CDS             complement(3157..4365)
                     /locus_tag="ACHBFA_001078"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9394587.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4477..4809)
                     /locus_tag="ACHBFA_001079"
     CDS             complement(4477..4809)
                     /locus_tag="ACHBFA_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9394588.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            4957..5832
                     /locus_tag="ACHBFA_001080"
     CDS             4957..5832
                     /locus_tag="ACHBFA_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9394589.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(5914..6495)
                     /locus_tag="ACHBFA_001081"
     CDS             complement(5914..6495)
                     /locus_tag="ACHBFA_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM9394590.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6501..7760)
                     /locus_tag="ACHBFA_001082"
     CDS             complement(6501..7760)
                     /locus_tag="ACHBFA_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9394591.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(7762..8808)
                     /locus_tag="ACHBFA_001083"
     CDS             complement(7762..8808)
                     /locus_tag="ACHBFA_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394592.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9043..9318)
                     /locus_tag="ACHBFA_001084"
     CDS             complement(9043..9318)
                     /locus_tag="ACHBFA_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9394593.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9561..10871)
                     /gene="der"
                     /locus_tag="ACHBFA_001085"
     CDS             complement(9561..10871)
                     /gene="der"
                     /locus_tag="ACHBFA_001085"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9394594.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(11053..12282)
                     /gene="rpsA"
                     /locus_tag="ACHBFA_001086"
     CDS             complement(11053..12282)
                     /gene="rpsA"
                     /locus_tag="ACHBFA_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9394595.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(12417..13097)
                     /gene="cmk"
                     /locus_tag="ACHBFA_001087"
     CDS             complement(12417..13097)
                     /gene="cmk"
                     /locus_tag="ACHBFA_001087"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9394596.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(13146..13781)
                     /locus_tag="ACHBFA_001088"
     CDS             complement(13146..13781)
                     /locus_tag="ACHBFA_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9394597.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(13847..15250)
                     /locus_tag="ACHBFA_001089"
     CDS             complement(13847..15250)
                     /locus_tag="ACHBFA_001089"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM9394598.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(15237..16268)
                     /locus_tag="ACHBFA_001090"
     CDS             complement(15237..16268)
                     /locus_tag="ACHBFA_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394599.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            16299..16520
                     /locus_tag="ACHBFA_001091"
     CDS             16299..16520
                     /locus_tag="ACHBFA_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9394600.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(16685..17422)
                     /locus_tag="ACHBFA_001092"
     CDS             complement(16685..17422)
                     /locus_tag="ACHBFA_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM9394601.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(17575..18915)
                     /locus_tag="ACHBFA_001093"
     CDS             complement(17575..18915)
                     /locus_tag="ACHBFA_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9394602.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(19152..20675)
                     /locus_tag="ACHBFA_001094"
     CDS             complement(19152..20675)
                     /locus_tag="ACHBFA_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9394603.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5983 a         4466 c         3491 g         6930 t
ORIGIN      
        1 taataacctc ttagcatttt tttcgtaaac agactatctt gctggatcat ccacgaagaa
       61 atcaaattcc ccgagttcat ttttattcct tcaagtttgc gttgctggat aaaaaattga
      121 accatcgttt catcataaat atcagtcaag ttatctaaac tttcaataat caactgagta
      181 tttttttgat atgtctctct gatttcttgg atatcttcat cactaataaa tgtctgacga
      241 tcagcatatc ttctttgcca aaacattttc ggatggagaa cgattcgcat taacctacgg
      301 acaacaatga accaaaagcc gataaaagac aaaatctggc ttgtgataga atgttcgaat
      361 cgagaaagaa aacgggagta aaaacgataa ccattagaag aaagcttgcc cgaacgataa
      421 ctttcatcca atccatcccg ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct
      481 tgcttttcat catcggttaa atcttccaag tacagatcac gtatccgttc ttgataattt
      541 tctatcactg cttcagtggc taaaaattct tttgtattac gatcaatttc ctttatttct
      601 tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc
      661 gcttcctctg tttccgtcag cataggcaag acaatgatcc ctatgaccaa agtcactaag
      721 atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt aaaacatcat attattgatt
      781 gaaaatggca aaataaaaat cgtagccaaa ctgactgttc cctttacacc gccaaatgtt
      841 aagatcaaga tctcgttcaa ctgttttttg aactttttac tgccatcttt gaatacataa
      901 aaaagactga tagaaatgaa tctgatcaca aaaagcatga cagagattaa tacgataatc
      961 gctaataaaa gaccgtttgg atataaaccg tctccccata ctggcgagaa cacttgcgtc
     1021 aattcgattc ctaaaaatat aaatactaat gcattcagtg taaaagcaat agttgtccat
     1081 gtactgtggg acaagctcga caattctgca tcaaacacag agattttcct aaaaccagaa
     1141 gcttggagaa tacctgctgc cactgcagca atgattccag atacatcaaa tgcctctgcc
     1201 aatacatagg ctagaaaagg aagcaaaagt tcgatcagca gataccctgt gacatcttgt
     1261 gctgatgctt tttcgatcaa atgaattatt ttttgtttga accagacaag caaaaagccg
     1321 atcaaagctc ccccaatact agaaactact agactcatcc ccgcgtttac tgctgaaaaa
     1381 gatccggtga tcagcgcacc aagagcaaac tggaaggcag taacaccaga tgcatcattc
     1441 aaaagacctt ctccttccaa aatatgcatc gcctttttag ggatattcac tcgtccagaa
     1501 agagaactga ctgctaccgc atctgtcgga cctaacgctg ctccaaaagc aaagcaggca
     1561 gcaagtggga cacttggcaa caggaatgat aacgtcgcac ctaccccgac aagggtcaaa
     1621 atgactcctc caaatgccaa gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata
     1681 tctgcttttt ctccttctct aaataacaat ggtgcaataa tcataactaa gaacaattca
     1741 ggttcaaaat cgattgattg tccccactct gtcagaccta ataccactcc aaggaaaatt
     1801 tggatcagtg gagctggaat cattggcaaa atgcggctag ctatgttcga taaggtgatc
     1861 gttactgcaa atacaataac taactcagca aattccatcg tccatcccct ttatttttta
     1921 ttttgtgtaa gcatttttaa aaattgttct tttttcttta aaattttggt ttcgactact
     1981 tcaacctgtg tttggttccc ctctaattca cactgtacac gttttgactc caaacgaagc
     2041 agatcatgtt tcagacgttc caactgacga tacatcaata tatttctttt acgcaactta
     2101 caaagtattt gacggcggtc atccatcgaa agggcgccat agcgatcatg caacagtttt
     2161 ttttgttgtt caaaactctc tctcattttc cagatctcct atacacaaac ttccatcatt
     2221 atagcaaagt tctttttgga tgaaaaatat ttcattgttt catcgtttat tgctagttag
     2281 aaatttccat aaatttttca acgcctcatt ctattccaaa tagtatcaga tatcaagtaa
     2341 aacttaagaa agataaaagt ttctgacaaa aggatgttta ctttcccatt cctgcctttc
     2401 ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta
     2461 ttctgaataa acggtgtgga agagacagcc tatcagtagt aataagacac atctctttca
     2521 cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat tccccgtata catcttctac
     2581 acgttgatct ggattgatca cgatatacaa gttacctgtt ttttcgctga tttttgttga
     2641 ttgatattga ttgtctaatc cttctgttcc tttatctaga tatgggaagt acacaagatc
     2701 aggtgtgcca tttcggtaaa ggatttccat tcgctcgata tcttcgtatt tcaatgcgcg
     2761 attaaacatg cccatttcca gtccgcctag gttgatatca tgtgtcgcaa ctgcgtcccc
     2821 ttcatggaag atttcgattt taaatccagc acacggatgg atttcaacaa attcactgcc
     2881 attgattcgt ccaaaacttg ttgtgatttg tttgatccaa agatcgccga tatgacggcg
     2941 gttgatcgtc caggtttctc catttctaaa ataaaatttt agcgctgtca tttctttact
     3001 cattaaaaag actcctttat gatgtaattt attcttgtac tacctcagtt tagcacaaat
     3061 ttatgtaagt ggtcacatct tatgtttttt cttctaatga cgtatctgcc cactttaagt
     3121 agccaacaaa gctgagcaga tacagtctag tgttttttaa atcaattttt ctttcgataa
     3181 aaaatccaaa acctgttttt tatcattttt tattctttgt tctacgacgg ctttttcagc
     3241 taattgcaaa gcttcactaa cccacttacc aggttttttt ccagtttctt ctagcaagat
     3301 tttaccatct actgctaatt cgcttaatga cttgataggt aaacgatcat aggattctcc
     3361 tacggcatcc atctgaggtg tttcgtcaaa atagatcatc aattcttcta cttgcagtgc
     3421 atgttttttg cctaagaagt acaattcgaa tggttccaaa gctccagttt ctcgttgtcg
     3481 tagtccttgg acaagtgctt gaacgtcttt aatcatttgg ttagaacact tccacgattt
     3541 caaaaaggaa cgaatctcat tttcttttag atccagttgg ctgatcaata atgcccatgc
     3601 ttggctctct tctttcaatg ggacggatgg taattcagca aaacgtaaaa gtgcttcgcc
     3661 ttcttctttc aaaccgggac agcaaatgta gcactctgtt tcaataaatg catggattcc
     3721 cgcggcccgt tcatttccca agagcatctt gatgaattcc acatttatac gttctacaga
     3781 tattttacct aatagctcgt ggttttcttt gatggcgtag aaggtttctt cctctaattt
     3841 gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt cgtaaggcat cttcatggaa
     3901 ccgctcatga ggattgccta cagctcgcaa gatctgttct tctagatctt gcagaccatc
     3961 gaacaaatcg atgatttctc catcttcttt caaagcaaac gcattgatcg taaaatctct
     4021 tcttttcaaa tcttctgcca acgaacgaac gaattcgaca tgatctggtc tacggtagtc
     4081 ctgataagtt gattccgtcc gaaatgtggt gatctcatat tgttcatcat tttctaatac
     4141 caacacagtt ccatgttcga tcccaatatc tatcgttttt ggaaaaagct gtttgatttc
     4201 ttctggaaat gcgcttgttg cgatatccac atcatggatt ggctggttca gcaagatatc
     4261 acgaacactt ccacctacaa aataagcttc atatcctgca ttctctaatt ttttcagaac
     4321 tggaattgcc ttttggtatt ccagaggtaa ttgttttaat ttcatttttc caacctttcc
     4381 tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg tgactgtttg acaagtccgt
     4441 ttcagagtct tcaaggccag gaccacttca tcttgtttac tcgtccttac gctcaaagcg
     4501 attatggtct ctttgattga atttttccat gtttttttca aaaacttcag ttaagtcgat
     4561 attcaatgaa ttagccatga tcatcgtaac gaataaaaca tcaccaagtt cttctgctac
     4621 cgagttaact ggttcagaag cctttttgct tttttctcca taagtgtggt tgacttctct
     4681 tgccaattca cctacttctt cagttaatcg agccatctga gctaatggag agaaataccc
     4741 caccttgaat tgctggatat attcatccac ttcctgctgc atactgtata aagaacgctt
     4801 tttatccatc tttttccctc caaaactatc ttaccaaaac tttaacgcaa atcctagata
     4861 agagcaattg agaaaatcga atttccatgc tacatttagg aaggaatttc taaatggaaa
     4921 ttctgtgaaa atacataatt gaaagctgga attcatatgg tcaaacgaaa aacagtaaaa
     4981 gaagttcttt ttatcattat aggaacaagt atttatgcat ttggtcttgt ttacttgaac
     5041 atcgctaatc atttagcaga agggggagtc tcagggatca ccttgatctt aagagcttta
     5101 tttggtattg atccagctta tacgacttta ttaatcaaca tcccattgat tttactagga
     5161 ggaaagatcc tcggtaaacg ttccttggcc tatactgttc taggtacact ttctttgtca
     5221 ttctttttat ggatctggca aagggttcca ctagaaatca atttacagca tgatctactg
     5281 atcgtttctc tacttgcggg tttagttgca ggtgtaggaa gcgggatcgt ctaccgcatg
     5341 ggtggaacaa caggcggcag tgatattatc gctcggattt tagaacaaaa ttacggcatc
     5401 actatggggc gttccctatt ggcatttgat atcatcgtct tacttgcttc attaacctat
     5461 atcgacttga aacgtatgat gtatacgttg attgtttcgt atgttttcag tcgtgtgatt
     5521 gattcgatct tagatggcgg atattctgca aaaggtctac ttgtcgtctc caacaaaagc
     5581 gaagagattg caccactact gatgaccggt ctgcaaagag gtgtgacttt cctttctgga
     5641 gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa
     5701 ttaactgaag ctaagaggat cgtccacgag attgacgaaa aagctttcct ttcaatcatc
     5761 aatgtgcatg aagtggaagg gcaaggcttt acttatttga agccacagac aagattactg
     5821 aaaaaactat aagaaaaaag acaaagatag gctatgagga aagattccac atccagctga
     5881 cctttgtctt ttttagtact atactatttt taactaattg ttatgttcac ttacccggtt
     5941 acttttcttt tgtgtctgca aaaggatctt tcggttaact tcatcagata atttcaaaaa
     6001 agcatcccta tctccatctt ctagtgcttg atcgatttga gcataaagca tttttagttt
     6061 tgcctcttct tctttttctt gaaaatatcg ttcaattaat tcagtatctt cttcactgac
     6121 tgaagaatcc caagaagaaa atggattgtc ttctaagata gatagatatt ctcttgtctg
     6181 ccaagcattt tcgaatatac attccacata taaaggcttt ttccaattca aacggatttc
     6241 atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc
     6301 cccttcaaca gatagatcac tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac
     6361 aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga taattcaaga tccaaaaaac
     6421 ttctcttcgg ctaaaagata catgattgac catccacgtt aaaaaattct tcttttcccg
     6481 tacatcaatt agcatttttc tcacctctct gttaggtcat ctaataatga cagtgcctcc
     6541 atatctcctg gttcaagtgt taaataataa gtcaataatt cttgcgctct tttcagctgc
     6601 ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat ctggttcatg tgatagttca
     6661 tgataagcct gttcatagtg tacagctgca acggtaaagt cttctaattc attataagca
     6721 tgggctaggt tccattctgc ataaggatta gcagtttctt ccatctgatt gatcacttgt
     6781 atcacatctt catatctttc atcatccaag taaagattac ttaaagtaag taatgtttca
     6841 tcttgtttgt ctcctacttc taaagctttc agtaaaagtt cttctgctcg ctgtctatca
     6901 tgcagacgga aagcattttc cgaggccagg tgataaagtt cgacttggta aggattttct
     6961 ttgattcctt cttctaacac tgtttgtgct tcttcaatca tttcttcttc ttgaagagct
     7021 tgtcctagat atagatataa cgattgataa tgcggattaa cttctcgaag ttcttccaga
     7081 tagttgattg cctgctgatt ttctttcaat tgaagattcg taaaagctag ctggaacagt
     7141 cgatcatctg tcctttcttc tttcagtgct tcttctaaag gtacgatagc tgcttcgaat
     7201 tctccttgca tacttaagga ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca
     7261 tttatcccgc ttgccaacaa tgtttcataa accactgctg cctctttgaa acgatcaact
     7321 gaaaaataca attcagctag cgcaaactgg atcaatggtt catcaggaag caacccagct
     7381 gcttctttca attttgcttc actcacttca gggattccga taacttggta caaatcagct
     7441 atcgccaata agctttgcgg atagtaatca ctttgcttat ccactttttc caggtattca
     7501 aaagctaagt cgatctcatt atcttcgatt gcaatttctg ctaacgggat gttcagtccg
     7561 tcattttctg ggtattggtt gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag
     7621 ccaatagcca gcaattcttc gcctagttca gctaatatat ctgctgggtc ttcttttaat
     7681 gctttttcta aaaagatttg tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt
     7741 ttttcgctat ttgtattcat ttcaattccc tcttatctca ctgacgattt gttgaaatac
     7801 cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg tatccgtcta ttatacctat
     7861 tttttctaaa acaagtattt ttttgctttt ttctgccaaa aaaagaactt tttccaaata
     7921 ctccatcttt gaaatctgtt ccagccagtg gattggatag ccaagcaagt ccagccacac
     7981 aaaaaagttt tttagttgga aatcaaaagg atatgcttgg agattccaca cgaaatgaaa
     8041 catcattcct aaaaattttt tcatactagc agaaagtaga tggccatttt cggtcagata
     8101 aaaagcttgt tcaaacagtt taccataatt tgacaggttt tctgcctcta attgataaaa
     8161 atagatcatt tcttcgatca tcccggcaaa cggccgagtc gttaattgct ttttatttgg
     8221 atagttttta tacagatttt gcaaaaaacg atgatcacaa atcattccgc aacagataaa
     8281 agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg ctgattgttt gatcataaac
     8341 gatgcgttct ggcagattga tggtttgcag cactacacga tttggtagct gcaaaatcgt
     8401 ccgctgcggt gttaatgctc ggcttagaga acggatcgat acagggatca tccatagtga
     8461 tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga tccatgatcc cttcatttcc
     8521 aaatccgatg ataaacatgg attgattttc gggatagcgt ttgacgaaat caagcaggtt
     8581 ctttaattct gttaaatgat tgcattgagt gttggcacaa atataccagt cattgtttgc
     8641 ttgatttgca aataacgggt tgatcttatc tgcaaacaaa tcataatagc gctgattcgt
     8701 taaaaataag attggctgat caatggaaag ttctcttagc tgactgacta gcgtttctcc
     8761 gtaaaacact ttcgtcaacg catcttctac ttgataatca agttccatac gcatccctcc
     8821 tcttcttatt tttattttac cattagtaag caaattctag aaaagataag agcaaagctc
     8881 acctcataac ttgcaaataa ataaaaatta aataaaatac atgtgttttc tatgtatgac
     8941 atacggattc ggaatctctg tctcttattt attccttttc tgctaggaaa taaaaagaaa
     9001 agcacttgta gccctagagc cacaagcgct ttttcaactg tcttatttaa cagcgtcttt
     9061 taatgcttta cctggtttga atgcaggtac tttgctagct ggaatttcga tttcttcacc
     9121 agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc
     9181 gattaattga actttttcgc cgttagctaa agcatcttga atagttgaaa atacagcatc
     9241 tactgctgca gttgcatctt ttttagttaa atcagtagct gaagcaactt tttcgattaa
     9301 ttctgctttg tttgccatgg atagattcac ctcctccaca agagatatga tagaaatatc
     9361 atttagttaa aactttctga gtggtccaga agtttaaaaa gaaatattga ttaacttcaa
     9421 tattctgtta tcacgatatc atatgaaccg cttaatagca aggtttttac caattttcac
     9481 atgctttttt tagtcataga aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa
     9541 acgtcgatat ggtaaaatgc ctattttctc ctacgaggga taattcggat ggacgtccct
     9601 tcaaaagtaa atgcttttct gatttgattt tctaagaaac gttcataaga aaaatgcatc
     9661 aattcctctt cattgacaaa aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca
     9721 taaaaaatct tcaggcgttt tcctttatct gttggtgttg gattgattgc tacagcatcc
     9781 ataatgatat cattcaacac agccgaagag atacgaagat tctggttcat acttacttgt
     9841 tcgatcattt caggcagacg ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt
     9901 ggagcgtaat caagataccg gaattcatca cgaatctctt gttcgaaatc acgcattgta
     9961 ttggtttctt tctttacaag atcccattta ttcacgacga taataattcc acgaccagct
    10021 tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa tcccttcttc agcattcagt
    10081 accatcaaaa cgatatcaga acggtcaatg gcacgcattg cacgcatcac gctgtatttt
    10141 tcagtatttt cgtatacttt tccgcgttta cgcataccag ctgtatctat cattaagaat
    10201 ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata
    10261 tccgaaacaa ttacacgttc ttctcctaaa atcgcattga tcaaagagga tttaccaaca
    10321 tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca
    10381 gagaaatact tcactgcttc atctaaaata tcaccaatcc ctagtccatg gcttcctgaa
    10441 acaggataag gatcgcctag ccctaaggaa taaaattcat agatatcgtt tctcatttct
    10501 ggattatcta ctttattgac tgctaaaatc actggtttat tacttctgta taaaattttc
    10561 gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc tgactacaca aatgattaca
    10621 tctgcttctt caatcgcgat ttctgcttga tgcttgatct gatccataaa aggttcatcg
    10681 ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc gacctaacca ttcacctttt
    10741 gcataaatac gatcacgagt tactccagga gtatcttcga caatcgagat acgctcacca
    10801 gcgatgcggt taaaaatcgt agacttaccg acgttcgggc ggccaacgat tgcaattgtt
    10861 ggatttgcca tattatttcc tcctcacaag tttccaatct cacttagttt aacgaatgca
    10921 ttgccaactt tcaagcatag attgtcaatt aaacgaagta tttttattcc ttattgaaat
    10981 actaatcgct tttcaaaaga aaaactgaga caaaagcagc tgctttcatc tcagtcacag
    11041 atacaaaatc aattattttt cagaatcatc ggttgctttt tcttctactt cttctttcaa
    11101 cgcatcacct aaaatgtctc ccatagtaaa tccagtattt tcttctggta attcataaga
    11161 ctcaacattt tttggttctt cttctgaagc tggtttttct tctaaagctt tgatactcaa
    11221 tgcaatacga tgttcttctg gatggatctc taatactttc actttcactt cgtcaccttc
    11281 atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa atttgagaga tatggactag
    11341 cccttctact ccagggaaca cttcaacaaa ggcaccgaag cttgtcaaac gtttaactgt
    11401 tccgtctaat actgtgccaa ctggagcttt tgtatcgatt tctgaccaag gtccaggctg
    11461 tgtgtctttg attgacaagg aaacacgttc ttgttcagga ttgatagaca acactttgac
    11521 atttacatca tcattgacag ccaatacatc acttggtttt gctacatgac tatgtgaaat
    11581 ctctgagaca tggactagac cgtcaattcc gcctaggtcg acaaatgcgc caaaatcagt
    11641 aagacgtgca acacgtccac tgagaacatc tccctcatgg atagttgaaa gaatttcttt
    11701 tttcttttct tctctttcgt tttccactac cgctttatgc gataagatca aacgattttc
    11761 tgatggttcg atttcgatga ttttaaagct caatgttttg cctttatagt ctgaaaagtc
    11821 tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa ccacgtacac cgacgtcaac
    11881 gactaaaccg cctttgacaa catttgtaac aggtgcttcg atgaccttgc cctcttggaa
    11941 atctctttcg atatcttccc aaactttttt agcatctaaa cggcgttttg aaagtagata
    12001 gctgccgttt tctttatctt tgccgatcgt actgataacg actaaatcta atacatcacc
    12061 gacttttact acttcgctga tatcttctac cggcaaagtt gataattctt ttgccggaac
    12121 gactccttca actccagccc cttcgattcc gacaatcact tgtttgtctt caatgactaa
    12181 aacttcgccc ttaacaacat cgccaacttt tacttcttgt acgcttttca ttgcgtcttc
    12241 cattgactgg ttgttctctt gattttgatc aaattctgtc ataacacttg tcctcctaac
    12301 caacattctg ccgtaagtac ggtactgttt ttagtttaat tgattattca aaaaaaataa
    12361 agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt catttttttt cttaatttaa
    12421 aaaagaagat aacctttttt caaaacaaca gcttttattg ctgcaacgac ttctggaatc
    12481 gatttgccag tcgtatcgat acgaacagca tcctctgctt gcttcagcgg agagacgtcc
    12541 cttgttgaat cataatggtc tctttcctgt atctctttcg tcagtgtttc aagatcagtc
    12601 ggaattcctt tttcttggtt ctctttgaaa cgtctttctg cacgctcact tacgcttgct
    12661 actaaaaaga ttttcacttc tgcattcggt aatacagctg tcccgatgtc tcttccgtcc
    12721 atgacaacgc caccgcgttc ccctattttt cgttggacag caactaattc ttctctgact
    12781 tttccatgtg cagagacttc agaaacagct tttgtgacat ctggttgacg gatttctaaa
    12841 gtcacatctt ttgatgctat gaatacatgc tgtccatctt cttgttgcga aaaagagata
    12901 ggatactttt tgatcagcga aactagccct tcttcatctg aaaaagatac attgttcgta
    12961 atagctaaat aagttactgc gcgatacatg gcaccggtat ctgtataaat ataattcaat
    13021 tcttttgcta atatcttggc aacggtactt ttcccagatg aagcaggccc gtcgattgca
    13081 atactgattt gtgtcatgat ataactcctt tttctcagaa aaaaagccgc aaagcgcggc
    13141 tattattatt taattcttaa ttcttgacca gggtaaagca taaagttgtt tggatctaag
    13201 ccattcaatt caaacaattg atcagtggtg atccctgcac gctcagccac ttgttttgga
    13261 ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt
    13321 tctgctgcat tgctgcttga atcccctgct tgataagttg cctcacttga ggaagtcggt
    13381 gttgtctctg ctgcaatact gctttctgac gttacacctg tatcttgaga cacagtagat
    13441 tcttccgcga ttgtactaga ttctactgtt gaagtctcag ttgactcctc ttttgttgta
    13501 cttgtagaac tttctactac tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg
    13561 ttgcttgatc catttctgat ccagaaataa gcacctgctg gaatagcgat acataagacc
    13621 agtaaaatca aaaatagcga aagaaataat gtatttcctt tcttttgttg tctctgagaa
    13681 cttcttgatg aaacttcatc atctgtatcg tagattggtt gttcccatgg ttcttgtgtt
    13741 tcgttatttt ctgtagattg atgtctatct tttctgctca ctgttttacc tcctaaactc
    13801 tatcgtgtgt attataccat aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt
    13861 tttttttaaa taaatttagt aaaatcgttt cccaatgaag ttgttcagtc ttagaaaaaa
    13921 gcttcctctt tggcagaagt tctgcttttg ctccatcaat atcacaacaa ttctgcggct
    13981 tttcggagag tttttcgcca aaataagcta agatgaattt tctgcggcag ttttcttcat
    14041 taatatagcg aagcatctgc tgcaggcgga attctttttc tttttcctga cgtttgagcc
    14101 gttccaccca attttctggt ttttcagact gctggatcag ctccatccat tttttttgca
    14161 gctcatcaaa gggagcttgc tctgtggcat attccaaata aagctcaaaa ctttgtcgct
    14221 gttctctaga aagttgatta aagaaataat ggatacgttc atccccactt tgataaaaaa
    14281 gaattgctgc actttcttcc tgatctctgc ctgctcgacc gatttcttgt acatagtttt
    14341 ctagactgtc tggcaaatca taatggacca cataacggat atcagattta tcgatcccca
    14401 taccaaatgc attcgtagca atgagaaact gcagctgatt ttttacaaac tgctgttgaa
    14461 gttgtcttct ttgagctgca tcaagaccac cgtgatagta tccaactgta aaacgttccc
    14521 tgaataaatg atacaatcgt tcgacttctt tttttgttgc acaataaatg attgccgctc
    14581 cttgtgctct ttccatgaag tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa
    14641 aaagcgagat attttgccga ttgactgatt gtctgatctc ttttggtggt cgatcaaata
    14701 gtacatgacg tatatctctt gctactaaat ctgttgctgt cgccgttagt gccagtgtga
    14761 ccgggtttcc taaaagcttt cggatttttc ctaactgttg atattctgga cggaaatcaa
    14821 ctccccattg agacacgcaa tgagcctcat ctactacata taacgcaata ttttgttttt
    14881 tcagatgctc taaaagagaa ggattagtca acatttcggg actcaaaaac aaaaatttat
    14941 attgcgatag atgacggagg acgtatcttc tttcagattg cgaaagtaaa ctattgaaag
    15001 cagctactcg tttctcccct cgtttttgta attgcgtcac ttgatcttcc attagagaaa
    15061 gcagcggtga cacgatgatg accagacctt ccattaaata ccccgtcaat tgatagcaaa
    15121 ggctttttcc cgttcctgta ggcaaaatag cgagagtatc ttctccatca agcaacgcct
    15181 gaataatttc ttcttggccg ggacgaaatg aatcaaatcc aaaatatttt tttaattcat
    15241 ttattaacat tctgtatcgc ttcctttctc aatagataga tttgatacaa acgaaattct
    15301 ccataatcta attgtccatc tacattccat tcgctaaagc gccactctcg cacggaatcc
    15361 ttatttggtt ccaaacgttc gattgtttct gcagagagaa tccactcgta tggaaaatca
    15421 tcaaaaaaca aggcccactc aataagatga tctgttactg ttcctctttt taaatgacgg
    15481 attgccatga tctggtcaat tgtctgacca ttcataaaca tctgcttggt gatcatcatg
    15541 ctttgattga aattttgctg taataatgaa tcgatcaaag cgtgccaaag cgaatctggc
    15601 cgtttttcga tttgtgctaa caaaaggtgg atacatctgc tttgttgcag ataaaccgct
    15661 gactcatcat tgttttttgc cagctgataa ggaagcagcc ctgttcgctc atttccagat
    15721 aattggttag ctaaaaaatc tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa
    15781 ctctcatacg cagtatcaat caaaatttca cgtggcaatt ggctttcact aagccatttt
    15841 tttagttgga aagtataaaa aggacttgtc tctgctggaa gatagtcttg aatacccgtt
    15901 gccagattag aaattacttg aacagcaaat ttaatgagcc gccaactagt ttcccctgtt
    15961 ctgccatagc ggtcaaaacg caaaccagta tgttctattt gatgttcgga caacatatca
    16021 gcccctgcag gcgttagctt tgcttcattt tcatttattt cgatccaacc ttgctgcgca
    16081 attttttgca aaatttgata aaacttatcc tgcttcaatt ctggaaaact gttatggata
    16141 aacaaaagtt cgtgggtaaa tccataaata agtactgaag tcgttcgttt tcccacaagg
    16201 agttggtaaa gagaactaat ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa
    16261 acaatcatat tattctcctt aagttaggat ggttagaaat gtcactacta tgtaaacttg
    16321 tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga agcgccagat attttcgatt
    16381 atgatgaaga aggaatcgtt ttatttgccc aagaaccaga ggctcatcaa cagttcgtga
    16441 tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt
    16501 tattagaaaa taaaatataa atataaacga aactttaaaa acgtacacta agttctctaa
    16561 acttgaaaat aagccgggat ctgtttctct tgttccgtga aatcccgact tattttgaaa
    16621 gattcatttg ctatctatag ttaattgact agttttttca cggaccctat aggacccatt
    16681 tatattattt caactttttc agccagtttt ccaatgtatt gacgatcaca gtttgctggt
    16741 ctgcgttaga aatttctgcc tctccatctc ctttttgctc accatagctg ccaaaacctc
    16801 catgatttcc gccattgatt gatacatact ctgttctttc cggtaaatac tgcttagctt
    16861 cttgataagc ttcctgattc aatactttat cttttgtcgc cgtgatcgat aaaacaggaa
    16921 cagctgattc tgtcaatgtt ccttttttgt cgggataact tgccatgaag aatacccctt
    16981 ttaaatgttt gtctgaatga ttcttattga cataacgact tgccattacg ccacctaatg
    17041 aatggccacc gatcacataa tcggaatttg gaaaatcttc tttgacctta tccgctttgt
    17101 ttccgcccat cactgctaga tcaagtggca ttttgacaat agcgactgaa aagccgtttt
    17161 ttgctaaatc ttgcgcccaa atactataac tttctggctc tacgagagca ccaggataaa
    17221 aaataagtaa tggagacttt ccatgattct ttttctctcc ttcaaaaaat aagtaatctt
    17281 ctttgttttt tccttctttt accaacactt gcgcttctga cgatggttcg tacgtatttt
    17341 gctgtatata gtaataaccg gctccaccga gtaatcctaa aatgatgact aagccaatta
    17401 gtattcgtct aaatattttc actttctccc accttttcta tctgagtgac ccctttttct
    17461 taatcaagta tgattatacc ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa
    17521 ataaaaaaag acggaatatc catttgaata tccgcccttt ttgtatgttt atttttattt
    17581 agcacataaa cgatacacag cttctgcata aatttccatt gctttataca tactacttaa
    17641 ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa
    17701 tgccacacac tggttcatcg tacgagcaaa agtagcgccg cctgatacaa acggttcagt
    17761 catgtctcca gtaatatcgc gatatgtccc caatagattt ttcactaact cactgtctag
    17821 tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa gtcaacccat atttttctgc
    17881 tgttttagtt aatttagcta ctaaatcatc tttcttgaat gtaacaggga ttctcatatc
    17941 tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt gcaaagttca tcgttagctc
    18001 gccggattgt tcatcttctg ttttgcccac gacagcttca ccagttgcat tttcttgaac
    18061 taattttcct aagaaattga ttgggccaaa atcaaatact tctgaaagag caattgccag
    18121 acgagaaata gcattcactc cttgtgctgc atctttcgcg tggatacttt taccaagaac
    18181 gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt tttttcaatg cttcttttac
    18241 ttcagaaagc ttttctccag agtaaggagc tgcatcagga acaacattca acgctcctcc
    18301 agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag taagcttgca ataagccttt
    18361 ttctgcataa attaaaggaa attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc
    18421 tttttcgtta tatttttcta aacaacgcca taaattttct tcatctgtac caaagataaa
    18481 acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa gctttgacgg catacattgc
    18541 cgcaatagaa ggacctttgt catcttggga tccgcgacca actaaccagc cgtcttttat
    18601 agttggatcg aatggttttg tttcccaatt attttcgtcc cctgctggaa caacgtccat
    18661 atgacacaag ataccgaata attcatctcc agagccaatc tctgaataac cgtaatagcc
    18721 ttctggatct ttaaatgttc taaatcctag cttttcactg atttccaata ctttatccaa
    18781 agcttcaatc acttttttac cgaagggatg tccttgtcct gaatctgctt catctagtac
    18841 ggaaggaatt cggatcaatt cgctcaatga ttctaatgct tgttcatgat gttgttctgt
    18901 cacaaatttt ttcatgatat tatcctccta atatgttaaa aagggtcatg tcaaattcct
    18961 ccgacacaac ctcttcttct caaataatga cgttttaagc ctgctccatc agcaagcaga
    19021 atgttcttaa caaatgatga aagaaaaaac actcactgaa tggagctatt gacttatcca
    19081 cgtgcttatc gatagccgtt tatcggtttc ttcattctta tgatagacct tgtacctgta
    19141 tttgtctagg tttagaacat tgctgctaca cctaggatca cacagatggc tacaaataat
    19201 atcgcaatta atttaccggt aaatttccac cacactgaaa catcgaaacg agcaattgct
    19261 acagcaccca ttacgatagc agatgttggt gtaattaagt ttactaaacc agatgcagat
    19321 tgataagcag tgattactaa atccttaccg acgccggcaa attcacccat aggtcccatg
    19381 attcccattg ttgcagcagc taagccggaa gttgaaggaa tcaagaatga catagggata
    19441 tagaaaataa atgtaataat gatgaataca cttgaagata atgaacttaa tccttgttcg
    19501 ccccaatgta agatggtgtc tgtgattaat ccattgttca taaccacttg gataccacga
    19561 gcaacagcta ctacgattgc tacaccgatc aaatcagaag caccagctaa aaattcagag
    19621 acaaattcag actctttcat gccataagtg attgctacta cgatggacat tactaagaat
    19681 aacatagtaa tttctgggaa gtaccagtca cctaatggaa gcatgctttt acctagaacc
    19741 gtacctagta ccggaaggtt tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc
    19801 gcattcaagg ttgtccaagg aatcaaactg atgatcatga taccaaaagt aataaagaac
    19861 aatacattaa catgtttttg ttttttcgtg attacttctt gacgaccgat tgcttcaata
    19921 tcgaaatgct tcatatcttc tttacgttgg ttgtatacta atgattttgt tggatctttt
    19981 tcgatctttg atgcataacg atacacatat acaatagaaa tagctaacaa aataacgaac
    20041 atcaagaatc gaagaccaat accttcaccc atcgaaatcc caacagcttg tgaagctacg
    20101 cctgttgcaa aaggatttac cgttgaagct agacagccta cttgagaccc tactaatacg
    20161 attgctacag ctactactgt atcaaagcct acagccatca tgactgggat cagtaaagca
    20221 tagaatggaa tcgtttcttc tgccattccg taagtagagc cgcctaatgc aaataacagc
    20281 atcaatactg gaataagcat tttttctttt cctttgaatt tcttcacaac tgaagcaatc
    20341 ccggcatcta gtgcccctgt tttgttgata atacctaaaa atccaccaac aaccaaaatg
    20401 aagaaagaga ttgggattgc agcaggtgtt tctttagtta gctcccccgc tggtacacct
    20461 agcatcccat tgattggcgc catgaaaata tcccacagcc cttgaggatt ttgttgtact
    20521 cgttcatatg atccagcgac gatgttgcct gcatcgtcta cactatagtg cccagctggt
    20581 ataaaccaag taagtacagc gattactgca ataataataa ataagaccgt atacgctgaa
    20641 ggcatttgaa attttttctt ttttgtttgt tccatcttct tcttcctttc ttttccgtta
    20701 gagacaacaa ttttggctct aatgttattt tatcaaaaag agtatcagaa caaaaataat
    20761 aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt ttccctattc cctcctcttt
    20821 tttcaggttt ttaaagcttt cttctttttc attgtagcgt ttcctttttt
//
LOCUS       DAYYNG010000037        20590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000037 DAYYNG010000000
VERSION     DAYYNG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.037"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            342..1031
                     /locus_tag="ACHBFA_001095"
     CDS             342..1031
                     /locus_tag="ACHBFA_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9394604.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1018..2226
                     /locus_tag="ACHBFA_001096"
     CDS             1018..2226
                     /locus_tag="ACHBFA_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9394605.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2520..3791
                     /gene="serS"
                     /locus_tag="ACHBFA_001097"
     CDS             2520..3791
                     /gene="serS"
                     /locus_tag="ACHBFA_001097"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9394606.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="ACHBFA_001098"
     CDS             complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="ACHBFA_001098"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9394607.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6329..7024)
                     /locus_tag="ACHBFA_001099"
     CDS             complement(6329..7024)
                     /locus_tag="ACHBFA_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9394608.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="ACHBFA_001100"
     CDS             complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="ACHBFA_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9394609.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8223..8414)
                     /locus_tag="ACHBFA_001101"
     CDS             complement(8223..8414)
                     /locus_tag="ACHBFA_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM9394610.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8435..9325)
                     /locus_tag="ACHBFA_001102"
     CDS             complement(8435..9325)
                     /locus_tag="ACHBFA_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9394611.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9312..10079)
                     /locus_tag="ACHBFA_001103"
     CDS             complement(9312..10079)
                     /locus_tag="ACHBFA_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9394612.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="ACHBFA_001104"
     CDS             complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="ACHBFA_001104"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9394613.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="ACHBFA_001105"
     CDS             complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="ACHBFA_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9394614.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="ACHBFA_001106"
     CDS             complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="ACHBFA_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9394615.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14650..15033
                     /locus_tag="ACHBFA_001107"
     CDS             14650..15033
                     /locus_tag="ACHBFA_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394616.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15201..15959)
                     /gene="jag"
                     /locus_tag="ACHBFA_001108"
     CDS             complement(15201..15959)
                     /gene="jag"
                     /locus_tag="ACHBFA_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9394617.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15977..16795)
                     /locus_tag="ACHBFA_001109"
     CDS             complement(15977..16795)
                     /locus_tag="ACHBFA_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM9394618.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="ACHBFA_001110"
     CDS             complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="ACHBFA_001110"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9394619.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="ACHBFA_001111"
     CDS             complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="ACHBFA_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9394620.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17923..19257
                     /gene="dnaA"
                     /locus_tag="ACHBFA_001112"
     CDS             17923..19257
                     /gene="dnaA"
                     /locus_tag="ACHBFA_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9394621.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19455..20585
                     /gene="dnaN"
                     /locus_tag="ACHBFA_001113"
     CDS             19455..20585
                     /gene="dnaN"
                     /locus_tag="ACHBFA_001113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9394622.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5885 a         4207 c         3788 g         6710 t
ORIGIN      
        1 gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa caaataataa
       61 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
      121 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      181 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      241 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      301 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      361 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      421 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      481 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      541 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      601 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      661 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      721 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      781 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      841 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      901 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      961 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
     1021 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1081 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1141 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1201 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1261 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1321 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1381 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1441 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1501 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1561 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1621 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1681 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1741 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1801 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1861 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1921 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1981 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     2041 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2101 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2161 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2221 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2281 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2341 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2401 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2461 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2521 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2581 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2641 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2701 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2761 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2821 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2881 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2941 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     3001 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3061 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3121 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3181 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3241 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3301 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3361 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3421 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3481 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3541 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3601 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3661 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3721 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3781 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3841 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3901 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3961 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     4021 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4081 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4141 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4201 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4261 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4321 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4381 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4441 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4501 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4561 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4621 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4681 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4741 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4801 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4861 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4921 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4981 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     5041 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5101 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5161 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5221 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5281 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5341 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5401 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5461 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5521 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5581 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5641 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5701 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5761 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5821 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5881 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5941 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     6001 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6061 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6121 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6181 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6241 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6301 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6361 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6421 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6481 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6541 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6601 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6661 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6721 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6781 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6841 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6901 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     6961 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     7021 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7081 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7141 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7201 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7261 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7321 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7381 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7441 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7501 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7561 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7621 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7681 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7741 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7801 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7861 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7921 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     7981 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     8041 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8101 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8161 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8221 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8281 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8341 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8401 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8461 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8521 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8581 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8641 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8701 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8761 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8821 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8881 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     8941 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     9001 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9061 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9121 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9181 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9241 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9301 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9361 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9421 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9481 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9541 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9601 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9661 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9721 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9781 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9841 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9901 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
     9961 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
    10021 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10081 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10141 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10201 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10261 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10321 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10381 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10441 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10501 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10561 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10621 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10681 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10741 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10801 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10861 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10921 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    10981 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    11041 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11101 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11161 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11221 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11281 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11341 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11401 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11461 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11521 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11581 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11641 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11701 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11761 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11821 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11881 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    11941 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    12001 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12061 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12121 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12181 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12241 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12301 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12361 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12421 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12481 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12541 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12601 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12661 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12721 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12781 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12841 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12901 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    12961 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    13021 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13081 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13141 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13201 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13261 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13321 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13381 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13441 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13501 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13561 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13621 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13681 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13741 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13801 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13861 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13921 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    13981 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    14041 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14101 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14161 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14221 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14281 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14341 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14401 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14461 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14521 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14581 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14641 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14701 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14761 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14821 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14881 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    14941 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    15001 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15061 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15121 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15181 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15241 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15301 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15361 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15421 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15481 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15541 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15601 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15661 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15721 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15781 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15841 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15901 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    15961 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    16021 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16081 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16141 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16201 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16261 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16321 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16381 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16441 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16501 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16561 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16621 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16681 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16741 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16801 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16861 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16921 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    16981 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    17041 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17101 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17161 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17221 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17281 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17341 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17401 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17461 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17521 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17581 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17641 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17701 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17761 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17821 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17881 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    17941 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    18001 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18061 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18121 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18181 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18241 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18301 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18361 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18421 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18481 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18541 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18601 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18661 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18721 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18781 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18841 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18901 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    18961 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    19021 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19081 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19141 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19201 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19261 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19321 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19381 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19441 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19501 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19561 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19621 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19681 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19741 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19801 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19861 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19921 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    19981 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    20041 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20101 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20161 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20221 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20281 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20341 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20401 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20461 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20521 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20581 attaacaatg
//
LOCUS       DAYYNG010000038        20314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000038 DAYYNG010000000
VERSION     DAYYNG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.038"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..702
                     /gene="purC"
                     /locus_tag="ACHBFA_001114"
     CDS             <1..702
                     /gene="purC"
                     /locus_tag="ACHBFA_001114"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9394623.1"
                     /translation="GKAKKIYLTSDENLIWIEYLDQATALNGARKDQISGKGELNNQI
                     TSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFSKRLGVPEGK
                     EFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINEILTELFAKV
                     GLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDLGDLIPVYQE
                     VYNRLSELTEEKERV"
     gene            702..956
                     /gene="purS"
                     /locus_tag="ACHBFA_001115"
     CDS             702..956
                     /gene="purS"
                     /locus_tag="ACHBFA_001115"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM9394624.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            958..1629
                     /gene="purQ"
                     /locus_tag="ACHBFA_001116"
     CDS             958..1629
                     /gene="purQ"
                     /locus_tag="ACHBFA_001116"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM9394625.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1630..3852
                     /gene="purL"
                     /locus_tag="ACHBFA_001117"
     CDS             1630..3852
                     /gene="purL"
                     /locus_tag="ACHBFA_001117"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM9394626.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3837..5276
                     /gene="purF"
                     /locus_tag="ACHBFA_001118"
     CDS             3837..5276
                     /gene="purF"
                     /locus_tag="ACHBFA_001118"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9394627.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5299..6351
                     /gene="purM"
                     /locus_tag="ACHBFA_001119"
     CDS             5299..6351
                     /gene="purM"
                     /locus_tag="ACHBFA_001119"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9394628.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6348..6926
                     /gene="purN"
                     /locus_tag="ACHBFA_001120"
     CDS             6348..6926
                     /gene="purN"
                     /locus_tag="ACHBFA_001120"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9394629.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6923..8458
                     /gene="purH"
                     /locus_tag="ACHBFA_001121"
     CDS             6923..8458
                     /gene="purH"
                     /locus_tag="ACHBFA_001121"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9394630.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8474..9748
                     /gene="purD"
                     /locus_tag="ACHBFA_001122"
     CDS             8474..9748
                     /gene="purD"
                     /locus_tag="ACHBFA_001122"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9394631.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9870..10784
                     /locus_tag="ACHBFA_001123"
     CDS             9870..10784
                     /locus_tag="ACHBFA_001123"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9394632.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10917..12482)
                     /locus_tag="ACHBFA_001124"
     CDS             complement(10917..12482)
                     /locus_tag="ACHBFA_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394633.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12475..13926)
                     /locus_tag="ACHBFA_001125"
     CDS             complement(12475..13926)
                     /locus_tag="ACHBFA_001125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9394634.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13940..14956)
                     /locus_tag="ACHBFA_001126"
     CDS             complement(13940..14956)
                     /locus_tag="ACHBFA_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9394635.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15176..16264
                     /gene="chvE"
                     /locus_tag="ACHBFA_001127"
     CDS             15176..16264
                     /gene="chvE"
                     /locus_tag="ACHBFA_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9394636.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16318..17844
                     /gene="mmsA"
                     /locus_tag="ACHBFA_001128"
     CDS             16318..17844
                     /gene="mmsA"
                     /locus_tag="ACHBFA_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9394637.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17846..19051
                     /gene="mmsB"
                     /locus_tag="ACHBFA_001129"
     CDS             17846..19051
                     /gene="mmsB"
                     /locus_tag="ACHBFA_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM9394638.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19308..20285
                     /gene="guaC"
                     /locus_tag="ACHBFA_001130"
     CDS             19308..20285
                     /gene="guaC"
                     /locus_tag="ACHBFA_001130"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9394639.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6495 a         3455 c         4660 g         5704 t
ORIGIN      
        1 ggaaaagcca aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta
       61 gatcaagcaa ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg
      121 aataatcaaa tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat
      181 tttattgaac aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg
      241 gaagtggtta tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa
      301 ggtaaagaat tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat
      361 ccatttatca atgaagagca tatctatttc ttacaacttg caaagccgga agaaatcagt
      421 gagctgaaag cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc
      481 ggtttggttt tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt
      541 gcggatgaag taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg
      601 gacaaagacg tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat
      661 aaccgtctat cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat
      721 atgtaaccta taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc
      781 atcgtatggg atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac
      841 ttgctgatgc agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg
      901 caaatgtaaa tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg
      961 aaatttgcgg tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt
     1021 cgcgagatca tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga
     1081 tttgatgggg ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg
     1141 attgctcggt tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca
     1201 gtattcggta catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta
     1261 ctgagacgaa atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc
     1321 aagacggcct ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat
     1381 ggggaaggaa attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag
     1441 attgccttta cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg
     1501 aacaaagcag ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg
     1561 ctaggatcag aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg
     1621 accgtataga tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc
     1681 tatgccgaat ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga
     1741 agaatgccta attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc
     1801 tataaaaatt ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa
     1861 ggccctggag aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa
     1921 gcagaaagtc acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt
     1981 ggcggaatca tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca
     2041 ttgaggttcg gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca
     2101 ggtatcggcg gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt
     2161 gatccttgtt atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac
     2221 aaacatatcc agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca
     2281 aaaacaggtc gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa
     2341 gaagaacagc aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt
     2401 gaagcgtgtt tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg
     2461 ggagcagcag gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt
     2521 atattgacat tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg
     2581 ttatcggaat cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc
     2641 caagaactat ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac
     2701 gggcaatatc ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta
     2761 gctgaagatg caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt
     2821 cagcagttag ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg
     2881 ctgcagcaac cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta
     2941 caaaccaata cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg
     3001 aataaagcgt tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa
     3061 atcggaggac aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag
     3121 cccttggcga tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg
     3181 gaattatcga cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt
     3241 atttcaggaa atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca
     3301 atgattggta tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa
     3361 aaatcagggg atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa
     3421 ttacaaaaga tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt
     3481 gaaaagagaa accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct
     3541 catgactgtt cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag
     3601 ctagggatat ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca
     3661 agatttgtgt tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat
     3721 gctgctgttt tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac
     3781 ggtcagatca aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt
     3841 cttatgaagt gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag
     3901 acgccgctag tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg
     3961 caggtatcgt gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt
     4021 ctgaggtatt caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac
     4081 atgtacgtta tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca
     4141 aatttcatga tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc
     4201 ttagaaaaga gttagaggaa aatggggcga tcttccattc taactccgat acggagatat
     4261 tgatgcattt gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt
     4321 tgaatgaagt caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg
     4381 ctttggaccc aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg
     4441 tgattgcctc ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc
     4501 agcctggtga gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg
     4561 acacgcagct ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca
     4621 tcgcagggat caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat
     4681 cgccagtaaa agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg
     4741 gttatgcgga agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg
     4801 cccgaacatt tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat
     4861 ctgctgtacg aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc
     4921 ggggtacgac aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc
     4981 atgtgaggat tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa
     5041 cgcgtaaaga actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg
     5101 cagattcttt aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag
     5161 atgctccgta ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat
     5221 atgactatga agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc
     5281 agaccaagaa gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat
     5341 gtcgaagctg gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt
     5401 ttaggtatga tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa
     5461 ttaaaagagc cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca
     5521 atcgaagcag acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat
     5581 atcttagcac aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat
     5641 gatccgttac gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat
     5701 atggcgctga ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat
     5761 gatctagccg gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg
     5821 atcaacccag gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac
     5881 tctcttgttc gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca
     5941 gaattgaaaa aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc
     6001 ttgcttccgc tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg
     6061 tttgtagaaa atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc
     6121 tcatggccga ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg
     6181 gagatgtatg aaatcttcaa tatgggcatc ggtatggtcc tggcagtcga agaatcgaaa
     6241 gtaaaagaaa tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa
     6301 gtgatcgcca aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg
     6361 tttttgcttc tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag
     6421 gattggaatc ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac
     6481 gtgcaactgc gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa
     6541 aagagtatga agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat
     6601 tagctggata tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca
     6661 tcaatatcca tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct
     6721 ttgaagctgg agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata
     6781 ctggaccaat catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt
     6841 tagaagaaaa aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta
     6901 ttgaaaatgg aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg
     6961 gattgtagca ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg
     7021 aacaaagcat gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg
     7081 gtttcctgaa atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct
     7141 tttaggaaga cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc
     7201 aatcgacttt gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt
     7261 cgaaatagca gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc
     7321 agcaaaaaac catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact
     7381 ttcagaatta cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa
     7441 agtctttcgt catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt
     7501 ggaagaaaaa gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta
     7561 cggtgaaaat agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc
     7621 gatcgcagca gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc
     7681 agatgctgcg ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca
     7741 catgaatcct tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta
     7801 tgaagcagat cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct
     7861 gccaacagca gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac
     7921 acaagaagct ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt
     7981 tgcgcaggaa gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt
     8041 acaagatcaa gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa
     8101 gccgactcca gcagaagcaa aagcattagc gtttgcttgg agagctgtca aacatgtgaa
     8161 atctaatgcg atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat
     8221 gaatcgagtc ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc
     8281 agttttagct agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca
     8341 tggtataaaa gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat
     8401 ggcgaataaa tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag
     8461 aggagtcgga taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag
     8521 taaaaaactt ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg
     8581 gatgacaaaa gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat
     8641 ccattttgtt aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt
     8701 aaatgggatc gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc
     8761 agcagctctg atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat
     8821 cccgacagct aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga
     8881 aaatggtgcg ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt
     8941 ggctaccaca gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt
     9001 tggggatagt agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct
     9061 tttggcgttt gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg
     9121 ggcatttgat aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc
     9181 gcaaatttca aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt
     9241 gggaatgaaa gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac
     9301 gaaggaagga ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt
     9361 cgttctttct cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg
     9421 tacagtagag ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc
     9481 cggttacccc gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct
     9541 ttctgtttac tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg
     9601 tctttattta gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac
     9661 cgcgttaatg gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc
     9721 tgccaatagc gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca
     9781 aacataatcg gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt
     9841 gttgtgtata taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc
     9901 agaaagcagc tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa
     9961 aagtgattga gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg
    10021 gaaacggtga atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag
    10081 aagatctttt tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta
    10141 aaaaacatat cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg
    10201 atgctttact tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt
    10261 ttgatgaatt ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa
    10321 tgcaaatcga attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat
    10381 ttggcttgaa acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag
    10441 caagtatcga tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc
    10501 aagcatggtc accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat
    10561 tccctgaatt aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg
    10621 gcttggcttc tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga
    10681 tgaacgtagg gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag
    10741 attggtacga attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag
    10801 gtcaaaaggg ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga
    10861 tattctgtct ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag
    10921 ttatttttgt ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt
    10981 ttcttaaata aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct
    11041 gaaatacgat agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc
    11101 agataatcga tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga
    11161 cttaatgcaa cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct
    11221 ttgataaaat ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag
    11281 ggcagagcag ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg
    11341 gcgctgattg ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc
    11401 ttttctttca ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt
    11461 gttcgagttg gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt
    11521 ttcgataatg aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt
    11581 ttcgcttctt gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt
    11641 tcaagtatca tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca
    11701 ggaccaatgg cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt
    11761 tgggctacct gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat
    11821 ttgatgaatc gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac
    11881 ttcaaataat cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct
    11941 aataatacgg tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat
    12001 gtttcactga tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat
    12061 tcgaaaacaa gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact
    12121 gctttttcta ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct
    12181 gcctgtacct ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg
    12241 agacgagtcc aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa
    12301 ggcatttgga tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc
    12361 atctcaccat ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt
    12421 atttgttccc gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac
    12481 atgtttgttt cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc
    12541 ttctcggtat ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag
    12601 acgcttgttt acgttgtaaa gtccgtagcc gatactgaaa tctgaatcga tccctttggc
    12661 taactcttta cgtatttctg ccagtttttc tgcagggatg ccaatcccgt tgtctataac
    12721 tttgaattgg atcatctcat caattttgca tgtggtaata cgaataaatc ctttgtgacg
    12781 gacgtacttc gttccatggt atacagcatt ttcaatcaat ggctgcaaga tcatttttaa
    12841 aatagaaaca ggaaaaagtg tttctggtat atcgatctta tagtcaagca tatggccgta
    12901 tcttattttt aagatgacta gataatcatt gatatggcta atttcttttt caagaggaat
    12961 ccagtcttgt cctttgctta aagacgtacg aaaaaagccg gaaagtgcat gggtcatagt
    13021 cagagcttct tcaaagcagt cttgttcgat caaagaaata atggcatcca aactgttata
    13081 aataaaatgg ggagtaatct gagcttgcaa tacacgaact tcactttgtg ctaaatggta
    13141 ttgtttcaaa gcgttctccc gtaaagactg gtttagatct tccgccattt tattcatgct
    13201 agcggtaagt gtttgtaatt ccagtgtttc cggcagggtt aaacgaaagc caaggttccc
    13261 tgaagaaagt tgttctgaca tctcgatcag ctgattgatc ggttcttgga cctttctctc
    13321 taaaaagcga cgattctttt tcgcatagaa aaaaatggta ctaagaactc caagttccac
    13381 tattgttaat agaatgagcg agcggacaag tgtcgtattt tttttgcttg ctaagtcgat
    13441 ctccacacga acaaaatctt gtaagatatc tgacagcagt tgagtgacag aatctacctg
    13501 attgatgatc tcttcattgg tatccataaa atcttttgcc aagatattat tgatcagctc
    13561 gttttgataa tcttccagtg tatcgatcgt acgtagcgat acatctaacg tactaatttc
    13621 tttaagtgta tttgtattat ttttgatctc tcgaatatca ttcctaagtt cttctaggat
    13681 attttcctca ttgaactcat ctacggttac ttggccagaa atcaagtccc acatactttc
    13741 caaaactttt tgatcaacct tcaaagcaat tgcatctgct ttttggatat ttgcgatcgt
    13801 ctgttgataa atcaaaagct gttgcgaata taacagtatg ctcagtagca aagggataac
    13861 agcaaataga agcatggctt tgtttgcttg ggcaaaaatc tgttttatgc ttaaattttt
    13921 tgtcatgata cttctccagt caataatttc tggttgggat gttagctata ttctgctggg
    13981 aaaagacagt ctcggaaata aaaatatctt taggtaatgt ttttcctgtt tgaatatttc
    14041 caaaataacg agtaacggta ttggctacat accagccgac atcaggttta cactcgacta
    14101 cacaattgac gattcctgag cgtagcttct cgatcatatc ttcttgtcca tcaatagtta
    14161 caatgatcaa atcttctcca ggtttgattg atgtttcttt tatcgcagct aaagcaccgt
    14221 gagtcatttc atcactatgg ctgtataata catcgatttc ggataaatca tttgtttcta
    14281 aatatcgttt catctggtct tttcctttca tccgaataaa atcaccgaca attcggtgaa
    14341 caatagtcat cggaggttta tggtcgatgg ttccatcttt ttttagggtt ggtttacgat
    14401 tgattgattc caaaaatcct tcggagcgta gcttggtcgg tgtggagttc tctgaaccag
    14461 caagttctag gattctgatt tcctgtttct ttgaatttgt gaagtgattg ctgacaaata
    14521 aacccgcccg atttccttct gctttgaaac tggggccaat gtgggtgacg aaaagctcgg
    14581 gatcattcgt ttgaatatgt ctgtcgacaa gtatgacagg gatccctgct cgctttgctt
    14641 cttttaatac agagtcccac ccggattctt gcaaaggagt aaaaatgatc atatccactt
    14701 tataggcaat gaacgtccgt atatcttgga tttgccgttc ctgattcatg tagccatttc
    14761 ggtagagaac ttggtaatct tctttttcaa gcgcagtgat aattgattca gtatgctttt
    14821 tccgccagct actctccgtt cctgattgcg aaaaaccaac gactagtggg tcaggtgctt
    14881 cctttgtatt aggtgtacac cccatcattt ggtaagcgat tacaacaagg gctaaaaaaa
    14941 ataaaacacg tttcacttta aatcactcca tctggatttg ttagtactat cttatcagat
    15001 aaaagggaaa atcaaagtca aaaggaatca atcgaaaaaa atccaagaaa cttaaaaaaa
    15061 tccaagacaa aaaaagtaca cctaaaaaat ttaaagtaaa tcccataaaa aaatcaatgt
    15121 taacgctttc tttattagct atactcggtg tatcaattga aatgggggat aaaaaatgaa
    15181 aaacaaattt agaaagattg ttgggactat cgctattttg accgcaagca ttctattaag
    15241 tgcgtgtggc aattcaggat ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc
    15301 gaccaaatca gcagaaagat ggatcgctga tgggaacaac atggtctcgg aattagaaaa
    15361 actgggttat aaaacagatc ttcaatatgg agaagataaa gtagaaaatc aagtagcaca
    15421 aatagaaaat atgattacaa aaggggtaga tacacttgtg attgcttcta tcgatggttc
    15481 ggctttaact gatgtgttag ctaaagcaaa agaagcagat atcaaggtga tcgcctatga
    15541 tcgtttactg atgaattcag aaaatgtcga ttattacgcg acttttgata attttggagt
    15601 aggtgtatcg caagcagctt atattgaaga gcacttagga ttgaaagaag gaaaaggacc
    15661 attcacgatt gaattatttg gtggctcgcc tgacgacaac aacgcactaa tcaattacaa
    15721 cggagtgatg tctgttttac aaccatatat ggacaacggt cagttggtcg tgccatctgg
    15781 acagacgagt ttcagtcaaa tcgctacgct tcgctgggat ggttctacgg cgcaagcaag
    15841 gatggataat ctgttaagcg caaattatac tgaccaaaca ttggatgctg ttttgtcgcc
    15901 atatgatcca ataagtttag gaatcatttc gtcattaaaa ggcgtggggt atgggagtga
    15961 aagtaaacct ttacctgtga ttactggaca agatgcgaca gttgctggcg ttaaatcgat
    16021 cattgccggg gaacaaacac aaacgatctt taaagacaca cgtatcttag caaaaaatac
    16081 gattgaaatg atcaaagcaa tcagtgatgg agaagaggtt ccggtcaatg atactgaaac
    16141 ttatgataat ggggtgaaaa ctgtccctac gtatttagca aatacagtat ctgtcgataa
    16201 agataattat caagcagagc tgatcgacac cgattattat aaagaatccg atctgaaaaa
    16261 ttaagaggaa ccaaacaagg aactaaagag atctaagtca gagagaggat aagaattatg
    16321 gcggattgta ttttaaaaat ggcagatatc accaaaaaat tttctggaat ccgagcgtta
    16381 gattgcgtga atctgatgat tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga
    16441 gcgggaaaat caacattgat gaatgtcttg agcggcttgt atccatatgg ctcctatgac
    16501 ggggaaatct tttatgatgg aaagctgtgt caatttaaag atttgaaaga tagtgaagaa
    16561 aaaggcatcg tcatcattca tcaagaatta gcactaagtc cttatctttc aataaaagag
    16621 aatatttttc tcggaaatga acaagcagaa agaggcgtga ttgattggga actgacagaa
    16681 aagaaaacat atgaactgct gaataaagtc ggtttgtcta tcgacccgaa tcttctagta
    16741 tcccagattg gcgtagggca acagcaacta gttgaaatag ctaaagcatt atcaaaatcc
    16801 gtccgtctgt tgattttgga tgagcctacc gctgcattga atgaagaaga aagcgcgaat
    16861 ttgctgagat tgatccgaga gttgaaagag caaggtgtaa cctccatcat tatttcacat
    16921 aagctgaatg aaatcgtgga tatcgcagat cgaatcacca ttttacggga tggacagacg
    16981 attgagacac tagaaaatga aatgatcagc gaagagcata ttattcgcgg aatggtgggc
    17041 agagatttag ttgatcgtta tcctgaaaga aaaccggata ttggagaagt ttattttaaa
    17101 gtagaagact ggacggtcta tcaccctctt gatcacgagc gaatcataaa cgaccacatc
    17161 aacttgcatt tgaaaaaagg agagattgta ggaatagctg gactgatggg agcgggcaga
    17221 actgaattcg ctatgagtct atttggacat tcttatggaa gcaagatatc cggacgtata
    17281 ttaaaggaag gggcagagat atcagttaag accgttcctt cagccattgc aaacggactc
    17341 gcttatgtct cggaggaccg aaaatcttta ggactgaatc tgctgatgga tattcgggaa
    17401 aacgtgacgc tgagcagttt ggataaaata agtcaaaaag gagtattaga taaagaaaaa
    17461 gaagtaactg aggcagaaaa tttccggaaa aagatgcgta taaaaacaaa ctccatccat
    17521 caaattgtca gtagtcttag tggtgggaat caacaaaaag tcgttttatc caagtggctg
    17581 atgacacaac ccgatattct ttttttggat gagccaaccc gtgggataga tgttggggca
    17641 aaatatgaga tctacacgat tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt
    17701 atctcttctg aacttcctga aatattaggg atgtgtgatc gaatctatac gatgaacgaa
    17761 ggacgtatta caggagaagt ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta
    17821 atgacgaaag aagaggtggg ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga
    17881 gaaaaaacta aatttgaaaa gtagagcagc tgatcttttt ggtcgctaca gcatggtgat
    17941 cattttagcg ggactactta ttgcgtttca attgatgacg gatggtattt tctggcgtcc
    18001 attgaatata actaaccttg ttctgcaaaa tagccatata ttagtactag ctgcagggat
    18061 gttattagtt gtgctcttag gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt
    18121 tggtgcaatt gcgggcatga tgatggtcaa tcgccaaatg aacccgtgga tgacagttat
    18181 cgcatgtctt gcagtaggcg ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc
    18241 agggataccg gcgtttatcg tgactttagc aggattgctg atgtttcgtg gactgacaca
    18301 agttgttcta ggaggccaat ctctagcacc ttttccagct gttttccaaa aaatgtctac
    18361 gggttattta ccagacttaa gcgaaggaag cagccttcac ttcttgacat tgattttagg
    18421 aatgatcatc acgatcgtgt tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa
    18481 tcatttattt gaagtggaat ctccaacagc tttcttggta aaggcggtat ttacagcagt
    18541 cgttgtacta ggaataactt atatctttgc ctcttatcaa gggtatccga ttattttgat
    18601 cattcttggt ttgatcgttg cagcatatgg gtttttgaca aataaaacag tagcaggtcg
    18661 acagatttat gcgacgggcg gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa
    18721 gcgaatcacg ttctgggtat tcgtcaacat gggagtcatg gcagctttag ctggattagt
    18781 tttagctgct cgattgaatg cggcaacacc tcaagcaggt acatcgatgg aactggatgc
    18841 aatggcggca gtatattttg gtggtgcttc gacttccggc ggcattggaa caatcatggg
    18901 tgcgattgtc ggtggcttgg tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt
    18961 cggggtagac tggcagcagg caatcaaagg attgatcttg cttttagccg ttgtgctaga
    19021 cttgtataat aagaaaaaac gaacaacgta ggagaacgcc ggttattcag aaaaaagact
    19081 gtgacaagac atttgtcaca gtcttttttt cgctaaaagc gaacattcat tgtttcgttt
    19141 gtatagacta aaaataatat atgttttttt accattcgta taaattctct ttttgctctt
    19201 gatttttttg cttaattgag ttaaattaca tagggcgcta aaaaaagcct tgattaaatt
    19261 aataatgttc gtgttttgct ggatgtgaac atcaggagga taatgtaatg aaagtatttg
    19321 attacgaaga tattcaatta attcctaata aatgtatcgt aaatagccgt tctgaatgtg
    19381 atacaacggt cacattaggt aaacatacat tcaaaatgcc agtagtgcct gccaacatgc
    19441 agacgattat tgatgaaaca atcgcagaat tcttagcgga aaatggttat ttctatatca
    19501 tgcatcggtt tgatgaagca gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat
    19561 tgattacctc gatcagtgtt ggcgtcaaga aagaggaata ttcctttata gagaagttgg
    19621 cagaagaatc gctaaatcct gattatatca ctatcgacat cgcgcatgga catgctaact
    19681 cagttattga tatgattcag catattaaaa aatatttgcc tgaaactttt gtgattgccg
    19741 gtaatgttgg tacaccggaa gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa
    19801 aagtcgggat tggtccagga aaagtatgta ttacaaaaat caagacaggc tttgggactg
    19861 gaggctggca actggcagct cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg
    19921 ctgacggtgg gatcagaaca catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa
    19981 tggtcatgat tggttcattg tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg
    20041 aaaatggaat cgtgtataaa gaatactttg gttctgcttc agaattccaa aaaggcgaaa
    20101 aacgaaatgt tgaaggaaag aaaatctgga tccaacacaa aggatctttg aaagatacac
    20161 tagtggaaat gcaacaagac cttcaatcat ctatttctta tgctggagga agagatctgg
    20221 aagccattcg aaaagttgac tatgtcatcg tgaaaaattc gatttttaat ggagacacaa
    20281 tctaaaaaat aaataaaata ttaggatgtt gtga
//
LOCUS       DAYYNG010000039        20309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000039 DAYYNG010000000
VERSION     DAYYNG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.039"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            292..1431
                     /locus_tag="ACHBFA_001131"
     CDS             292..1431
                     /locus_tag="ACHBFA_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM9394640.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="ACHBFA_001132"
     CDS             complement(1498..2073)
                     /locus_tag="ACHBFA_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9394641.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="ACHBFA_001133"
     CDS             complement(2078..3052)
                     /locus_tag="ACHBFA_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM9394642.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="ACHBFA_001134"
     CDS             3335..4303
                     /locus_tag="ACHBFA_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9394643.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="ACHBFA_001135"
     CDS             4416..4694
                     /locus_tag="ACHBFA_001135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9394644.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="ACHBFA_001136"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="ACHBFA_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9394645.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="ACHBFA_001137"
     CDS             5151..5906
                     /locus_tag="ACHBFA_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9394646.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="ACHBFA_001138"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="ACHBFA_001138"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9394647.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="ACHBFA_001139"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="ACHBFA_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9394648.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="ACHBFA_001140"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="ACHBFA_001140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9394649.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="ACHBFA_001141"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="ACHBFA_001141"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM9394650.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="ACHBFA_001142"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="ACHBFA_001142"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9394651.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="ACHBFA_001143"
     CDS             10643..11185
                     /locus_tag="ACHBFA_001143"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9394652.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /gene="atpA"
                     /locus_tag="ACHBFA_001144"
     CDS             11208..12764
                     /gene="atpA"
                     /locus_tag="ACHBFA_001144"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9394653.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="ACHBFA_001145"
     CDS             12778..13680
                     /locus_tag="ACHBFA_001145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9394654.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="ACHBFA_001146"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="ACHBFA_001146"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9394655.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="ACHBFA_001147"
     CDS             15123..15545
                     /locus_tag="ACHBFA_001147"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9394656.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="ACHBFA_001148"
     CDS             16014..16244
                     /locus_tag="ACHBFA_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9394657.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="ACHBFA_001149"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="ACHBFA_001149"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9394658.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="ACHBFA_001150"
     CDS             17655..17831
                     /locus_tag="ACHBFA_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9394659.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="ACHBFA_001151"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="ACHBFA_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9394660.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="ACHBFA_001152"
     CDS             18326..18709
                     /locus_tag="ACHBFA_001152"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9394661.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="ACHBFA_001153"
     CDS             18878..19762
                     /locus_tag="ACHBFA_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9394662.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="ACHBFA_001154"
     CDS             19876..20112
                     /locus_tag="ACHBFA_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9394663.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="ACHBFA_001155"
     tRNA            20200..20280
                     /locus_tag="ACHBFA_001155"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
BASE COUNT         6748 a         3550 c         4414 g         5597 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaagg
//
LOCUS       DAYYNG010000040        19730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000040 DAYYNG010000000
VERSION     DAYYNG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.040"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1148
                     /locus_tag="ACHBFA_001156"
     CDS             105..1148
                     /locus_tag="ACHBFA_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9394664.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="ACHBFA_001157"
     CDS             1321..1545
                     /locus_tag="ACHBFA_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394665.1"
                     /translation="MNQRRRKRLEEISERLSKLKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="ACHBFA_001158"
     CDS             1548..1805
                     /locus_tag="ACHBFA_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394666.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2022..2897
                     /locus_tag="ACHBFA_001159"
     CDS             2022..2897
                     /locus_tag="ACHBFA_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394667.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2907..3701
                     /locus_tag="ACHBFA_001160"
     CDS             2907..3701
                     /locus_tag="ACHBFA_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394668.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            4000..4848
                     /locus_tag="ACHBFA_001161"
     CDS             4000..4848
                     /locus_tag="ACHBFA_001161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394669.1"
                     /translation="MIEYDFNEAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKV
                     RPYENLKTYMFFLVNFEGTAKEGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLI
                     LDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQK
                     SLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRS
                     EVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKGFENKGSFGKSKDEVKGVVIDDD
                     FEFDFD"
     gene            4881..5201
                     /locus_tag="ACHBFA_001162"
     CDS             4881..5201
                     /locus_tag="ACHBFA_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394670.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5456..6145
                     /locus_tag="ACHBFA_001163"
     CDS             5456..6145
                     /locus_tag="ACHBFA_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFM9394671.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6182..6349
                     /locus_tag="ACHBFA_001164"
     CDS             6182..6349
                     /locus_tag="ACHBFA_001164"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394672.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            6369..8003
                     /locus_tag="ACHBFA_001165"
     CDS             6369..8003
                     /locus_tag="ACHBFA_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394673.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDSHVNMSLTIKRIYDLLYVYKH"
     gene            7975..9201
                     /locus_tag="ACHBFA_001166"
     CDS             7975..9201
                     /locus_tag="ACHBFA_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394674.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10051..10446
                     /locus_tag="ACHBFA_001167"
                     /pseudo
     CDS             10051..10446
                     /locus_tag="ACHBFA_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            10524..11563
                     /locus_tag="ACHBFA_001168"
     CDS             join(10524..11021,11024..11563)
                     /locus_tag="ACHBFA_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFM9394675.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            11576..12514
                     /locus_tag="ACHBFA_001169"
                     /pseudo
     CDS             11576..12514
                     /locus_tag="ACHBFA_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            12507..12854
                     /locus_tag="ACHBFA_001170"
     CDS             12507..12854
                     /locus_tag="ACHBFA_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394676.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            12851..13033
                     /locus_tag="ACHBFA_001171"
     CDS             12851..13033
                     /locus_tag="ACHBFA_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394677.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            13169..13789
                     /locus_tag="ACHBFA_001172"
     CDS             13169..13789
                     /locus_tag="ACHBFA_001172"
                     /inference="COORDINATES: protein motif:HMM:NF037736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFM9394678.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFFRNSN"
     gene            14032..14592
                     /locus_tag="ACHBFA_001173"
     CDS             14032..14592
                     /locus_tag="ACHBFA_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFM9394679.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            15481..16248
                     /locus_tag="ACHBFA_001174"
     CDS             15481..16248
                     /locus_tag="ACHBFA_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFM9394680.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            16274..16819
                     /locus_tag="ACHBFA_001175"
     CDS             16274..16819
                     /locus_tag="ACHBFA_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394681.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            16820..17386
                     /locus_tag="ACHBFA_001176"
     CDS             16820..17386
                     /locus_tag="ACHBFA_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394682.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            17401..17649
                     /locus_tag="ACHBFA_001177"
     CDS             17401..17649
                     /locus_tag="ACHBFA_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394683.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            17668..17907
                     /locus_tag="ACHBFA_001178"
     CDS             17668..17907
                     /locus_tag="ACHBFA_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394684.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            17924..18508
                     /locus_tag="ACHBFA_001179"
     CDS             17924..18508
                     /locus_tag="ACHBFA_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394685.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            18615..18896
                     /locus_tag="ACHBFA_001180"
     CDS             18615..18896
                     /locus_tag="ACHBFA_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9394686.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            18886..19227
                     /locus_tag="ACHBFA_001181"
     CDS             18886..19227
                     /locus_tag="ACHBFA_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9394687.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         7473 a         2648 c         4042 g         5567 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc caagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagcta cttttttcaa
     1621 ataagtacac agccaaagag ttggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaaggaatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggat ttcaagctga caatgattct aaaaaagttg
     1801 aatagtttaa tagtcgccaa gaaaagtacc aggacgaggg gaaaggtaga ttacaaagga
     1861 aaacactttt aaataaactt cgcaaaagca tcaggttatg agtggctagg gctatacgaa
     1921 tgtgatgaaa acaaagggct ttaatgtcaa agaaaacgca ataggggggg aacaaaattt
     1981 ttaaaaaaga tatttatgtt tggtttaatg gaaaaaaata tatgactact aacaaaaggt
     2041 ggtcagagct agtacaaaat acagttaaaa aagtcatgcc agatggatat catttcaact
     2101 atatacaatg ctcacaaaca actgaaagta tttatttttc aatattaaat gagaatagac
     2161 tctattattt cagggtaagt tcacaccata ggaaaaagag agaagataat gttacgacat
     2221 ttttatcggg aaaatacaat ggcttcaaag agttaagtga agccattggt gagtatctaa
     2281 aaagtaatag atttgtcaga atttctgaca aacatatatt gatcttgtta ctttatttga
     2341 ataatagctt caaattgttt tggagagacg acactttcta ctatattgag aacggcatgg
     2401 atatttattc tggtatcaac ggcatattaa aacattatat taaggcgtta cttcgatatg
     2461 gggttcttta tcaagaagat ttaagtaacc aaattaagat tagtaattct ggaaagtcat
     2521 tactggcaaa atataaaaaa ttgctttctc ctatctacaa aggaatagat atggaagctg
     2581 taaattctga aactttattt aatttagttg ttgatgaaaa gtttgcgaat gaatttttta
     2641 aaagtaaaaa aattgaaaga ctaaataacc tgtttcctga aaatgcaata aaagcttatg
     2701 tagataataa taaatcacga ttatacaact ggtttattcc caaacggttg cgaggaaaaa
     2761 tcaaaattgg cgatcaagtg aaagtaatta atctaaatca tactaagatc gttaccgtaa
     2821 cggagttgta tatagctgaa aaagaagata ttacaaaaat gaaaccagtt attaaaatac
     2881 tagataacaa agagtgagtg gaatatatgg tagcaaaaaa gaatactcta aaagaagctt
     2941 ctgaaaaata tcaagatatg aaaagacgag gattaatacc aaaggacaaa aaaggtatat
     3001 ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg cggtgacttt gttggacgat
     3061 atacagcaaa aggttatagc aggcgcttaa ggatcactgt tgaatatgga acgaatgaat
     3121 cagctacaga gctgaagcaa aggtacttag atacacgaag agaagcttca aaaaaatatg
     3181 atgatttcaa gagcagaggt ctaattccta agaagaaaaa ggggatttgg cgtgagtttt
     3241 tcaaagaaga agatagcaat cggaataagg gagacttcat tggacgatac acagcaaaag
     3301 gctacactaa gaatttaggg atcaaggttg aatacggaac tggtgaaagt tcatgggaat
     3361 tgagaaatcg ctatttaaat acattgttag agaaatataa agcagaatta ttggagttgt
     3421 tcgatgttaa tgattttatc gagatggctg aaaagtatgg tcatgaagaa ggttttatga
     3481 tcaatgacat tatggttatt gataatttta cggatttatt agcacttgcg aaaaaagcaa
     3541 gggatttaag agaaacgcag atgtatctat tcagaaataa aaacggaaga aaccggagaa
     3601 ctgaatcgag tgagattgaa gattactatg atgcctttac acacgatctg ggagcctttg
     3661 tagacgagat aagtggtgta atgaacaaat atagggtcta gaataaaaaa gccggagatg
     3721 agcagataaa tgggaaacaa tagcatatac acacctgaaa tgcagaataa aattaaaaga
     3781 cttgtcggat tagggtggat gacaagagga aaattagatt cttttagtac gatcaaatat
     3841 cttttgatgg acatttaccg tcagaacaag caaacaggaa tacatttttt gggctatgat
     3901 aagccggaag atgcaccttt ctttgttcca agttcagcct atagagccag tacctccggt
     3961 agcatgtata acttggattt gtttgaggaa ggagcaaaga tgatcgaata tgatttcaac
     4021 gaagcctatt cgaggatcat gagaaattat cctttgccgt caaacactta tttgaaagca
     4081 aaatataata cagatcaagc tattttgaga gcgataaata gaccaaaagt aagaccgtat
     4141 gaaaatttaa aaacgtatat gttctttttg gttaattttg aaggaacagc caaagaagga
     4201 acatatgcta atccaaaatc acagtcgatg tttgcggatt atggcgaaaa tcccaaaaca
     4261 gctaatgagc ctatttggtt atcggagatt gagctaacac ttatccttga ttattatgat
     4321 attaaagaga tggaagtcct agatagccac acatttttga cgaaaaaagg attactcaaa
     4381 gaatattttg aaagaatcga gccattaaaa actgatcctg atacgttgaa gctttataaa
     4441 aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat cattaagaga aggagaaaca
     4501 agcaccttcg ataaatacag tatgtataat cgagcgtatt cctccatggt agcaggcgta
     4561 tttagagatc gtatggttag atatgaacag aaatacgttc atagcgcata tgggctatta
     4621 aaaattcgta ctgatggatt gtatttcaga tccgaagtac cagagtttga agagctgtat
     4681 aaacttggag tagttaagaa aaaagttcat acaatatcca aagaagaagt cgaaaaagct
     4741 ttagaattta aaaacaaagg atttgaaaat aagggctcct ttgggaaatc taaagatgaa
     4801 gttaagggtg ttgtgataga cgatgatttc gagtttgatt ttgactaaat actctttgga
     4861 aaagatggta ggaggtaaat atgaagaaga aaattgtgga aggaaatgta ggtgttatta
     4921 ttctaaaaga ttcaactgac tatgctaaat caatggcact aaaagacgaa aaagttgcac
     4981 gcatggataa agatttagtt gaatttctga tggatgatac agaaagatta aaaggggatc
     5041 agtttttgag tttaatgaaa gacaaaggct taaacacata tggtttattt gattataaga
     5101 aattagctat agagtgggta aaagaaggag aaatattttt tattgaagaa tgggaaggta
     5161 ctgagaactt agttcttcat cagtatcttt tgaaagctta atctaagtaa aacagttctg
     5221 actagttatg cttgtcagaa tttcaaagga agttttccat aaccatcatc attcaaaaat
     5281 aaaggatttg aaaataaggg ttcctttggg aaatctaaag atgaagttaa gggtgttgtg
     5341 atagacgatg atttcaagtt tgattttgac tagatttttt tgcaatgttt aagagtaaac
     5401 aaggtaagaa ctaagatcat gaagattgag agtaaagaag atagaggacg aatgtatgag
     5461 aacacactat atttatttag aagataaaat tgtagatgaa attccggcaa aaagtcgaga
     5521 acaagctaga aaattattta ttgaaaaata caaagagatc aatggagata gtgaagttcc
     5581 aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag agaagtacgt ttcatgtcag
     5641 ccctgatata tataatctta tagagcgaca tagaagtgaa gataatattt ctatctctca
     5701 atatattgct acagttattg atgaattgat ttcaggaaag tatgactatg cttttgattt
     5761 tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa tctgattttg agaagctaaa
     5821 aaactatgct ggcaggagaa atgttaattt tgcaagacta ctagaagagt tctactccga
     5881 agagttggga gtaggaaatg aatacaagag agaaacttat gcgaaattgt ctcataagtt
     5941 atcccaagcg cttaaagaag tgacagaagt taaagcggaa ctagatgcta aacttgaaga
     6001 agtgaaaaaa tatcaaacag agaggaagaa caacatgaag aatcaagtgg aatttgacga
     6061 acagaaagaa tttgaaaaag caagaaaacg ctataacaaa attatgaatg aacttagcta
     6121 tgaacactta acagttcatg aataattgag tgagggtaca gaaaaatata cgatcgcaga
     6181 tatggttaat gaagtggaaa ctcttttagc ttctttcttt gaaaatggtc acgtaaacga
     6241 tgaatggcga aaagaagaac ctaaaagggt taacgcttat attgctcgat taaaaaggtt
     6301 tattgataaa tataaggaga atagtgacaa attttctaaa gataaataag gagagagtgt
     6361 gattacattt gccagaaatc gaaggaatat tttcgccaaa aataggtaaa aaacagttag
     6421 atttcagcag ttttccgagc gtaaatagag attataatct aggtgatgag gtacagatac
     6481 taaatattga tttcaaagaa ctagacttcg ctgttgctgt ttccaacaaa cccaaggcat
     6541 tggaacgagt gaaaaaaata taccaagaga atgatcaaaa tatcaaaaat atgatcaaaa
     6601 agtatagacg agataattca aaagttgaag acgttttatt caacggtacg gcaaaacatg
     6661 atttgaatgt ttatatggca ttaggagtgg ctttgtactg taattcaatt aatcagacag
     6721 atattttggt taatatcgtg aggaatagct atcaaaaaat attaaaaata agtagcgaaa
     6781 caaaccctta ttatgcaatg aaatatcttg gggataattt attaaatgat aaaaaattga
     6841 tgcacaacac tgaagagctg ttatctaatt tggctgcgat taactttcta ttcaaaacag
     6901 gttctgaaga ttattttata gataacgtac taaaaggtgc ttacggaaca gcattttatc
     6961 cgctttacaa tatgaaacag cataaagccg aacaaaagga taacaaactt gaaattaaaa
     7021 agatgaaaga actattaggt ataggatttg taaaacccaa ttcaaaagtc actattgctt
     7081 cgttagcaac tactaacaat gatgggaaaa tagtaaatgg gtttgacgat tcagatatag
     7141 atatattgtc aaaatcatat ttttctttgt atggagattt tgcagatttg atgggtattg
     7201 atagttactt agtcaataat tttgagatca agggtagcga gctgaatgat attgtgtatt
     7261 tcgttttaat ctctctatat gcaattgatc agaataccga gtaccgacaa attgactatt
     7321 tgctagtttt ttatagtttg ttatatcagt tcgttttaac tcggttgttt aaaaatgcta
     7381 aggaagaatt gatcctgaat tctgattatg aaatagcaaa acaaattgca agtattagaa
     7441 aagaaaacaa atctgaagta atcaaattaa gaaaagaatt aagtattgcg agtaaagaaa
     7501 acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga agaggaactt ttgaagcaag
     7561 agattaaaga attaaaagga atcagaaaag aacttgaaca gctgaggttt gaaaataaag
     7621 tattaaagca attgcaggag caagaacata gacaatcatt aaatggtaaa actgaagagc
     7681 cttcactgtc tgaaatgata gcttatctaa aacataaaaa cattctttta gtgggaggac
     7741 atgtaaattt tcaaaataaa ttaactgaac tattaccaaa tattaaaatg tttgatgtaa
     7801 gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg tgatgcgatt tttctatata
     7861 cagattactt gaatcatggt ctatattatc gtgttatgag tgaaatttct aataaagata
     7921 agatatttta tttggacagt catgtgaata tgagcttaac aattaaacgt atttatgatc
     7981 ttttatatgt ttataaacat taatgaggtg ctaaaattga caacaataac tatgacaaaa
     8041 aaaatagaag tatatgttga tggatcacaa gaagaaaggg attccttttt tgattttttt
     8101 tggaaaaata gtagagaata tgtaaaagcg ttgaatctgg tatataaaca gctaatgttt
     8161 gaagagttga taaaagatgc aattaaagaa atggatcaag gttatgctga gagagaagaa
     8221 agtattcaaa atcggattga aaagttagca tatactgatc caatagatca aaaaaaactt
     8281 gataaagcaa aagctgaatt atctaaattg agaaatgaaa aaaataaaga agcgagagaa
     8341 atattaacgc aagcgatagg gttgaagagt caaacgagaa cacgagatgt agcaaaaaaa
     8401 ttagactttg actttggaga ttttgttgat tccgcaaata tgaaagctag taaagatttt
     8461 ggcaatgatt ttatcggaat tgtgaccgga caaagaacgc ctagaatata taaaaataga
     8521 aacaaatatg gattactacc ttttagaggt cgagatatta aaatccataa agatgataag
     8581 ttttatattt atttacctaa aggctttagg tttaaagtga atttaggtag caagccaaga
     8641 aaagcaagag atgttgcttc agttttacaa cacattatgg atggcgatta caaattaaat
     8701 caaagtaatc ttatttttga tggaagaaaa ttatttttta atctaacaat ttcttttgaa
     8761 aaaagctata atgaaatgga attcaagaaa ggtaatgaat tgatcgtaaa ctttgcagat
     8821 ttgtgtagcg gtgaatgttt aattaatgat aaatctgtaa tgtcttttgg tgatagccaa
     8881 tatataagaa acttcaaaag taaaatttta gcaatgaaat caaaagaaca aagccgagga
     8941 gtcttttcta agggcggtca tggtcgaaaa agaaaattag atcaagatcg atgggaagca
     9001 ataaagaaaa gagaaagtaa ttttgttaaa acttataata atcaatgttc tgcaaaaatc
     9061 gttaaaactg ctattcgcaa taaagctcaa attattcgta tcattgaacc agataaggct
     9121 tatggcgaat gggcttacta tcaattgaaa gaacaactag taaacaaagg aaaaagatat
     9181 gggatagaag tcagaagtta gtaaattaaa caatcgttga tttggcaacg aaatagtgag
     9241 ggaaaatcca ctcactgaag ggtaaactcc gtcaagttgt gtgaccagac ggaaggacga
     9301 atgttgttga ggttttagaa ccatataaaa cagcatgttt ctcaaatttt taggataagg
     9361 gaaattgcta gcagtttgtt ttagaaccat ataaaacagc atgtttctca aatgagtact
     9421 ttaaattaat tgatggcgtt cctgctttag aaccatataa aacaacatgg atttaaaaca
     9481 agaagtaagt cagttctcct tttactttag taaggaaaaa taaagtattc tcaataaaac
     9541 ttaaaataag caaacctttg ttttaatagc taatcgaagt aaaacccaaa gccatatgaa
     9601 cccctgaatc agcgaaagtt tatgatgatt tggattacta atagtttagg aattagatgg
     9661 taaatctaaa ttaaacaatc gttgatttgg caacgaaata gtgagggaaa atccactcac
     9721 tgaagggtaa actccgtcaa gttatgtgac cagacggaag gacgaatgtt gttgaggttt
     9781 tagaaccata taaaacagca tagttctcaa attgtttaaa atgattgtca agttaaaaaa
     9841 atctgggatc atataaaata gtatagttct caaatatgat tagaaaccct attaaagagc
     9901 aatattaagc caacttactt aaatcgttta attaagaaca ataatctctt ttagtcagac
     9961 ttttagtctg acttttttgt atttccaaaa tttattcttt acaaaaacga acatacgttt
    10021 gtataattgc ttgtgaatgg gggaataaaa atgtttgatt acagtagaga accggtgaat
    10081 gactattttt taatcgacat gaagtctttc tatgctagcg tggaatgtat tgaacgaaat
    10141 ttagacccaa tgactacacc gttaattgtc atgagtagat cggacaacac tggttcaggt
    10201 ctgactctag cttcttcacc tactgcaaag aaaaaatatc atattactaa tgttagtaga
    10261 gctactgatt taccttatcc attaccaaaa gaacttcata ttgttccacc acgaatgaac
    10321 ttatatataa aaaaaaatat ggaagttaat cagattttta gaaagtatgt cgcagatgaa
    10381 gatttattga tttattcgat tgatgaatca attttaaaag tgacaaaatc cttaaattta
    10441 tttactatat aaattgagtt aaattgtatt cgtttttcct cctataggcg tatactaaag
    10501 aaaacacttg aggagtgggg aacatggaaa cagaacgcca aatccgtgaa ttggaaaaag
    10561 cgatgaatac gacagacaac cgccggttgt acgagcgttt cctggcagtc aagttggttc
    10621 ttgaaggcca cacccggaaa gaagccgggc acaccatcgg ccgcgacgag catactgtca
    10681 gccactatgt gaaaaactac tgccataatg gaatccaagg tttagtctcc aaaaaacaaa
    10741 gcggacgacc ttcacggctg acagatgaac aggaagcgga actggtccaa atcgtcgcct
    10801 accacactcc ggaggaagta ggtttcaaat cccgtgccaa ttggacattg gcgattgtgt
    10861 gtgacctgat tctccgggaa tggagttatg gatatacgca aaaaggcgtg gcggacctcc
    10921 ttcaccggct aggactaagc tggacccgtc cgacgtatac cctcaaaaaa gcggattcca
    10981 agaaacaaca ggtatttttg gaagaaacct tcccgagcct aaaaaaaaat tgctgaatag
    11041 cgatattgac cacctcctat tcgaggacga aaccatgatc cgggattacc aagccattgg
    11101 ctgcacctgg tttctccgcg gccaccagcg aaaaatcccc acttatggac aacacaaagg
    11161 ggtaaaattg ataggcacct tggattatga aaccggtcgg atcatttgta cagaagaagg
    11221 ggcatacgac gcagcggcct tcctccgttt tctggagcgg acgttgcggg aataccccac
    11281 cggaaaaatc gtgatggtat tggataatgc ccttatccac cacgccaagc tgatccagtc
    11341 ttttcttgaa gaaaacgcta ggcgcctggc actcatcttc ctccctccct acagccctca
    11401 gttgaaccta atggaaggcg tttggaagtg gctcaaggaa tcggtcatca acaatgtttt
    11461 ttttgaccac gtccagaaaa tcaaacagtc ggtccgcggt ttcttagccg atgtaaacga
    11521 gcgtcccctt gaggtgattg accgactgtg cgtgcggatg taaagtattt aattcaacct
    11581 atatagttca ggaacaagaa gcgagagaag aagagaatta gctaaaataa tcaaagatga
    11641 gattcagaaa gaaacaggtc taatagcaac agtcggaata ggagataacc cattattagc
    11701 taaacttgca ctggacaatg aagcgaagag tagtaaagaa tttatagctg agtggactta
    11761 tgagactgta cctgaaaaaa tatggaaaat aaacgagatg actgactttt ggggcattgg
    11821 atcacgcatg aaaaaaagat tgaatcatat gggaattatg agtatagaag atttagcaaa
    11881 ttggaatccc tacacaatta aaagtcagtt aggtgtaatc ggactccagt tatatttcca
    11941 taccaatgga attgatcgta cggatattgc tattccacca gaaccaacca aagaaaagtc
    12001 ttatgggaat tcacaagttc taccaaaaga ttatacgagg agagaagaag tagaactagt
    12061 agtcaaggag atggcagagc aagtagccat tcgaattaga caacacaatt gcaaaactgg
    12121 ttgtgtacgc ttagcaattg gtacgtcaat tttggaagaa aaatcaggtt tctcgcacca
    12181 gatgaaaata ccaattacag ataatacgaa agaattacaa aattattgtc tttttttatt
    12241 caataagttt tatgaggggc aggaagtacg acatatagga gtaacttata gcagattagt
    12301 atacacagat tccctacaat tagatttatt tagtgatcca agaaaaaaga ttgctgaaga
    12361 aaatttggat aggattattg ataagattag acaaaaatat ggatttacag ctattataca
    12421 tgcttcatct atgttagaag gtgcaagaag tattacgaga agcactttaa taggaggaca
    12481 cgcaggagga ttaggaggtt tgcataatga ttgaatataa cgatagagga cgtttaaaat
    12541 ggtcgggatt ttatttgtct gatcatactg aaagaatcaa tgcagacaat gtacaacgtg
    12601 ctaatcataa tttggcaaaa gaacaaatga cgactgaaga gattgcgaaa atactgagaa
    12661 ctgctgtcat aaaaaataga cccgtctgca ttcaaaaaga ggaacgaaac gctgaaggtt
    12721 actatccaga tgatgtagta ggaaaaatta ttggatatga cgagcttggt gtatttgtgg
    12781 aagaagaaaa agttcactat gatgaaatta ggaatgtttc tttctcggaa ttattaaaat
    12841 ggagtcagat atgaacatta ttaaaaaata cgaattcggt tatattacgt atgaacagct
    12901 aatagaggaa ttatggggat atggtcaaca attaattaat gaagtgggga ttgattgctt
    12961 ttgtttctat attgaatccg gagcaggata ccataagaac agatactata tatttccata
    13021 tccaccaaaa tagtctctta gatattttta aattttctcc aaatactttc aattattgtg
    13081 aaatatttcc ctttaattaa tcaaattttt tataaaagta atatataaaa taaattgctt
    13141 gaagtattat atttagatat cataatttat ggaagaaaaa ttaattaaaa aggatgagat
    13201 tataatgggg ttactggttc cgagagctaa tacttatact atacctttaa cgaaacgcca
    13261 attaattgct aaaagatatc aaaggattac tagagccatc aatagagagt tttggaattc
    13321 tgaaagtgat actgctcaca gtttatacgt aggttcttat gggagaggaa cggcaattag
    13381 tactagtgat atagatataa tagttgaatt accgatggca gaatttgaca gattcaaaaa
    13441 ttatttatca aatggcccgt ctaagttatt acaagttata aaaaacgcat ttcaggaaat
    13501 actaccaaat agtgatatta gagctgatgg acaagttgtt aaaataaatt tccatgatgg
    13561 aatcaaattt gaaatagttc cagcttttaa tgaaaaagat tattggggtg agtcaaaagg
    13621 gtttatttat ccagattcaa acatgggagg gaactggaaa gctacaaatc ccaaaaaaga
    13681 gcaagaagcc atgaaattaa agaatacaaa aagtaataat ttattatatg ctacgtgtaa
    13741 acattttcga catgtacgag atacagaatt tttcaggaat agtaattgac agtttcgtat
    13801 atgaggctat gggaaattgg aaattcgttg aaaataacag tgggggtcaa aacattagtt
    13861 ctgtatctta tgagacagct ttattagaat actataactc acataaagtt atgggcggtt
    13921 taaatttata ctctcctgga agcaatcaat ttgttaattc tgatagtagt atcatatgtt
    13981 tagaaaaagt attaaaaaaa atagctttat aaagttttat ggaggaacaa catgggagac
    14041 gaaaaaaagc gcttggaagt tcaattacgt gaagcatatg gacgagtaac gtatacttac
    14101 accactcatt taaagcaagc cgataatatt atttctgaca ataaaatttt aaggtgtctt
    14161 caaattggtt tgtcagccct tactactgga ggggtactta gtaccattat tactgataat
    14221 agtgagttaa cctttgttgc tggattatgt tcaacaattt ctcttggatt aaacttatac
    14281 tttaagaatt ttgatcttgc tgaagaagct aaaaatcatc aaattgttgc cgataaatta
    14341 tggttgatca gagaagaata cgtaagctta ctaacagatt taaatcgttt aagtgttgaa
    14401 gatattgtga aaaaaagaga tcaattaatg aacgaaactc atgaagcata taaaaatgcc
    14461 cctaaaacaa atagtaaaag ctatgaatca gctcagaaag cattaaaatc tgaagaagag
    14521 caatttttta cagaagagga attggataag atgttaccag agcatcttag aaaaaagagt
    14581 tctaaatatt gaactctttt tttatgctta aaattctctt gtaatttttc tattcatcta
    14641 tatttaagaa gaaatatgat gatagaaaca tctcctaaag ccaccgcaag ccgttctaag
    14701 catagcttag cccgacattg ctagatatgc tcatctcacc atgaacgttt cctaaagcca
    14761 ccgcaagccg tcctagccat tgcctagtcc gacatcgcta gattagtgca tctcgccata
    14821 aaagttttct aaagccaccg caagccgttc taagcatagc ttagcccgac attgctagat
    14881 atgctcatct caccatgaac gtttcctaaa gccaccgcaa accgtcctag ccatcgccta
    14941 gtccgatttt gcttgatcgg ttccatttct gtttatttag tgggtcaaca ccgtaatctt
    15001 aaatagccat cgcctattta agattactag gtctgctttg aaggtaaaaa gtctttcatt
    15061 cttcaagact tttaggcttt caagctacgc ctgaaagcat tgaaattcat cgctctttat
    15121 ttggttttgc catttataat tccttacgcc cattatcttt ttcgcctgtc ataccttgtc
    15181 agaagtgttt agagcttatc tcatctttgg attaatacaa gtggcgtaaa tttttttcta
    15241 aacgcaagcg ttacgaaaat aatttactta aacacttgta ttaatccggc agataagaac
    15301 gcattctaat cactcccgac aaggcaagac aggcgaaaaa gataaataaa tgaacgaaag
    15361 gaagaaacat aaaatgcaaa accaaatagc gatgaagaaa aaaccacctc cctctaaaac
    15421 aatccaaaca aaaaagaatt ttgaaaaacc aaaaatcaaa aacagaaaga agcgttcaac
    15481 atgagagata gaattttaga agttaatcaa gaaatggtaa acaagttaac aaatatcagt
    15541 gggaactatg aaaagtacga tggtacttta cttgataact atatttttca cgatacacaa
    15601 aatatcagaa ttggcagaag taaaccgaga gcgttcattc tcatcaaaga gaaatatttg
    15661 aatgaatgga atagcaccta tgaaatggtt atgactgata actttgaaaa ggtattggat
    15721 ttcagaaatg ctttgttggg ttctgatatg ggaaatagca gtcttgaatc tattgaagtt
    15781 gaaaacaaga ccgagaatga aggtgaagga ttgattaaga atgtcttgaa aatcgaacaa
    15841 aaatatatca gtgaagatag aagtttttat agagtcacaa gctcggttgg tctagcaaac
    15901 tttgctacta acttaattgg aaacaatgct agtgaatctt tgctagtgat gtgtctgaat
    15961 acaaagaatg aggtcattgc ttatagcgaa gttttcaaag gtggaattaa tcaatcagta
    16021 gcgataccaa gagagatttt tcaactagct ttgctaaaca attcagcaaa aatcgcagta
    16081 tcacataatc atccgagcgg atatgaattt ccaagtgaaa atgacaaaag ttttactacg
    16141 aatttgaaaa acgcaggaaa aacaattggt attgaattgt tagaccatat catcgtaaca
    16201 agtaaaaaca caagctatta ctcatttcaa gaaaatggtt ctttataaaa attagagaaa
    16261 agaggaaaac aagatgggcg aaagaacaca agcgttaatt acagtcaaat atggtaacag
    16321 caaaacgaaa gcaagcgtgt cgattcatta tcagtggggg tatggtcgat atatgctgat
    16381 ggacgtattg aatcaaggaa tttatttaat caataaagca tacgacttac gagataaatc
    16441 aagagaagaa ttagcaaaag gaatctttga tagaagtaca ggagttatca attttgtcac
    16501 ctatgaatct ggagtgagtg cttatgatga aacagggttt ggtgggcgct atgaacctta
    16561 tcaagaaacg attgaagatg tgatgaagtt ttcagataac aatgatggct atgctcattt
    16621 agagatggtg ttcgatgaaa atggatttag tctagatgat aaaaccagat tgcgattgtt
    16681 tgattttgag ggtcaacaga taggactaaa caagttttgt gaaagcggaa aagagtttgc
    16741 tacgaaagat tttattaagg gatacagatt attgctagaa aattatggaa taaacctaga
    16801 agaagagagc gtgaaataag tgtttgaaaa aagaaaagac cagttcccta atttagaagc
    16861 gaaaagtcaa aaagtgattg atggtgaaat gattgaatta ttccaaaaaa gttatggaac
    16921 catagaaact accgcaagtt gcaaatacta ctttcaagga aagagaaaca cgtttaaagg
    16981 ctttgccgat tctgatgaag aattggcaaa gaaaatcatt gattggcttg tcagcagtga
    17041 acacgtagca agagaagata ccgttagaac aacgattaat ggggaagtcc taacaggtac
    17101 tagatgtgtt tgttgtaatc atgaatttgt agaagatgaa ggttgctacc gaatcaatga
    17161 atatggtgat tttatttgcc agagttgcca cgagatagaa tttccaaccg aaagaagttg
    17221 gtttgaatac atgctacaag attatgagat ggaaacagaa aatggtgaag tagttttttg
    17281 taccaaagag gaattgagta agagaagcga tgatgaaatt gaagattggt gtgtgtggtt
    17341 agtagggaca gatgattgcg ttgcatatta cacaacagca gaataggagt gtgaatcagc
    17401 atggaaggaa tggattttat agatcatgaa gatttgattg attttggtta tacatggaaa
    17461 ggaatggtag gtattagcag aagtcttgct aatgcctttt acgaaagaaa ttatgcagtc
    17521 tatgtccttt atgatgacaa tacagaaagc cttgttgatg aagagtataa attggattta
    17581 gagaatgttt tatatgggat agaaaaagaa gatttagcaa aatatatttt ttcatggttg
    17641 ggtcaataaa cgaaagaggg tagcaacatg acaaaattca aaagtaaagg tatgcaatgg
    17701 tgttcttatt attatgttgg gaatgatgaa gagcttttga gaaaagccaa acaaaaaggt
    17761 tatccagata agatgttaaa acagattggc tattacatgc cagaagagag aatagaaaaa
    17821 gaatcttgta aggttctttt tgatagtgaa cagctattag ttttgttaac tcaaaataaa
    17881 ttgatttgtc aatttgaaaa taattaatat ggggtgaaga accatgaatg agaataagat
    17941 ggtattaagt gaatacgcaa gattgagaac cattaattgg ttgaatggta tgcaaagtag
    18001 tggcgctttt gatggatttg atgaggagaa tatggacttt gaaagtttat cgaatgagga
    18061 tattagatat tattttatcg aatggtattt tacagaacca gatgaagagg tggaagatga
    18121 accagaatta attttagaac gtcaaagaat tttattcagt gaacaagata gtaagaggtt
    18181 gtatggttta tatgacttac aactgaaagg attaaaagcg cagtatgttg ctgatagtta
    18241 cgatgatatg gtagaacagg gggcagatag ggttgtcact tattcagaag atgatgaaga
    18301 ggaatgggaa aactattcag atgatgagat atgctttaat gatgggtata tgcctattga
    18361 aatcaccgaa gaagaatatg ataaagtgtc ggatagcgat gagattggta tgtgtacggt
    18421 tgtaagtgga ttggatatag atgtccaaag agtagaggtt gcataccaga agatgaaaga
    18481 acgtataaaa agtaaagtag aatcttgatt tgattggatt gtttgcaaag tagctaatta
    18541 agagtataat ataatcataa atgtagttaa tggatataat aatgagtata ttatactcaa
    18601 aagaggtgaa cgaaatgaag gtaaaaaatc caaaagaaaa aagcagaatt caagtcggaa
    18661 tagacaagga tttaaaagaa aacgcagaaa tgatattgga agagctaggg ttaaatccaa
    18721 caacagcaat cacaatcctt tataagcaag tggtcgctag aggtgagttt ccagtagaga
    18781 taaagctaag cgaagaggaa aagcaaggaa tcagattgca acagctaact atggatatgc
    18841 cagttgatgt tctagataca gatgaaaagt tagaagagtg gttcaatgaa gcctaatgag
    18901 atagtaacgg tctatgttgc atttacagat aaaaatggtg gtaaaagaag acctattttg
    18961 gtggttagtg ataaagaaga tagagtagaa ttttttggaa tcacaagtca atatgagaat
    19021 aaatcagacc aaataaagag agtgtatttt cctattgcag aatgggaaaa agtaggtcta
    19081 aagaagcaat catggattga cgtagggtct ttaaaggcta ttcccaaaag ccgagagaac
    19141 atatcattta aaaaggttgg tatgctatca gctattgata cgaaaaagct aaataggttt
    19201 atcgagagat tacaaagaaa agtctaacaa ggcttttctt tttttgttta tatgtatatt
    19261 aagatatacg tttatataaa cgtatttatg tatattaaaa tacacgtaaa tatatacgtg
    19321 attataaaaa ttaagaaaat attaagtatt gttaaatagt gataattagt aatatacgaa
    19381 ttataagata cattttatat ttaatgtatg taaataaata aaatgactga cggatattga
    19441 atagtaataa ataacaataa ttcagacgga tacagtgagt gaagtagctt gctacgaacg
    19501 aacaggcaga atgaaacggt agtttcatta gagcaagtcg cttgcgacgt agcgttacca
    19561 ctgatgtaac tggcaagtta catagagaga gtttgcttgc aaacgaacgt tacccacttg
    19621 aatagaaaac aaaaataaca tattagtgaa ttttgaatag gagccacaac tcaaccaacc
    19681 gcttgcggtg ggtgagtccc acggaggaaa agacgaacga acggtcgcgt
//
LOCUS       DAYYNG010000041        19631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000041 DAYYNG010000000
VERSION     DAYYNG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.041"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1370)
                     /gene="purB"
                     /locus_tag="ACHBFA_001182"
     CDS             complement(75..1370)
                     /gene="purB"
                     /locus_tag="ACHBFA_001182"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9394688.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1383..2507)
                     /gene="purK"
                     /locus_tag="ACHBFA_001183"
     CDS             complement(1383..2507)
                     /gene="purK"
                     /locus_tag="ACHBFA_001183"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9394689.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2500..2988)
                     /gene="purE"
                     /locus_tag="ACHBFA_001184"
     CDS             complement(2500..2988)
                     /gene="purE"
                     /locus_tag="ACHBFA_001184"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9394690.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3404..4711)
                     /locus_tag="ACHBFA_001185"
     CDS             complement(3404..4711)
                     /locus_tag="ACHBFA_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9394691.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4714..5295)
                     /locus_tag="ACHBFA_001186"
     CDS             complement(4714..5295)
                     /locus_tag="ACHBFA_001186"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9394692.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5411..5508)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5867..7159)
                     /locus_tag="ACHBFA_001187"
     CDS             complement(5867..7159)
                     /locus_tag="ACHBFA_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9394693.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7156..7779)
                     /locus_tag="ACHBFA_001188"
     CDS             complement(7156..7779)
                     /locus_tag="ACHBFA_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394694.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7782..8618)
                     /locus_tag="ACHBFA_001189"
     CDS             complement(7782..8618)
                     /locus_tag="ACHBFA_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394695.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8635..9522)
                     /locus_tag="ACHBFA_001190"
     CDS             complement(8635..9522)
                     /locus_tag="ACHBFA_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394696.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9538..10821)
                     /locus_tag="ACHBFA_001191"
     CDS             complement(9538..10821)
                     /locus_tag="ACHBFA_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9394697.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11504..12187)
                     /locus_tag="ACHBFA_001192"
     CDS             complement(11504..12187)
                     /locus_tag="ACHBFA_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9394698.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12205..12822)
                     /locus_tag="ACHBFA_001193"
     CDS             complement(12205..12822)
                     /locus_tag="ACHBFA_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9394699.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12832..13656)
                     /locus_tag="ACHBFA_001194"
     CDS             complement(12832..13656)
                     /locus_tag="ACHBFA_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM9394700.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13649..14209)
                     /locus_tag="ACHBFA_001195"
     CDS             complement(13649..14209)
                     /locus_tag="ACHBFA_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394701.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14383..15339
                     /locus_tag="ACHBFA_001196"
     CDS             14383..15339
                     /locus_tag="ACHBFA_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9394702.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15644..16600)
                     /locus_tag="ACHBFA_001197"
     CDS             complement(15644..16600)
                     /locus_tag="ACHBFA_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9394703.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16704..17306
                     /locus_tag="ACHBFA_001198"
     CDS             16704..17306
                     /locus_tag="ACHBFA_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9394704.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17394..18893)
                     /locus_tag="ACHBFA_001199"
     CDS             complement(17394..18893)
                     /locus_tag="ACHBFA_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM9394705.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18909..19433)
                     /locus_tag="ACHBFA_001200"
     CDS             complement(18909..19433)
                     /locus_tag="ACHBFA_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM9394706.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
BASE COUNT         5659 a         4321 c         3404 g         6247 t
ORIGIN      
        1 catcagtcct tattgacaaa gagccggtta tttttgctat tctattttaa ttgaattttg
       61 caactcactc ttctttattc caaacctact cgtttgaata tcacgtcaac gtttttcaaa
      121 tgatgatgat aatcaaaggc atcgtctatt tcttctttag ataataccga tgtgatggtt
      181 ggatcttctt ctaacagcgg gcggaactgc acttggttgt cccaagcgta cgcagttttt
      241 ggctgaacaa gatcataagc ttcttctctt gtcattccct tatcgatcaa tttcagcata
      301 acgcgctggc tataaatcaa accaaatgtt gcagacatat tccgcttcat gttttcagga
      361 aagaccgtta gatttttaac gatattgcca aaacgattta acatgtaatt caataaaatc
      421 gtggtatcag gcaagatgat tcgttctgct gaagaatgag aaatatctct ttcatgccat
      481 aatgtgacat tttcataggc agtgaccatg tgtccgcgca ccactctggc tagaccagcc
      541 atattttctg aaccgatcgg attccttttg tgcggcatag cagaagaacc tttttgacct
      601 ttcgcaaaaa attcttctac ttcacgtgtt tcggattttt gcaatccgcg tatctctgtt
      661 gcaaaacgtt caatacttgt agcaataagc gccatcgctg agaaatattc agcatgtaga
      721 tcccgaggga gaacttgagt agagatttct tgtggacgga tccccaattg tttacataca
      781 tactcttcaa taaatggcgg gatatttgcg aacgttccca ctgctcctga gatttttcct
      841 gcttctactc cttttgccgc atgttcaaat cgttcgatgt tccgtttcat ttctgaatac
      901 catgtggcaa gtttcaatcc aaaagtagtt ggttcagcat ggacaccgtg tgtccgaccc
      961 atcatcactg tatatttgtg ttcttttgct ttttcgccaa tgatttccgt aaaacgtttc
     1021 aagtcttccc gtaaaatatc atttgcttgc ttgatgagat agccataagc agtatctacg
     1081 acatccgtac ttgttagacc ataatggacc cattttcttt cttctcccaa tgtttcagaa
     1141 accgctcgtg taaacgcaac gacatcatgg cgtgttaatt tttcgatttc caagatccga
     1201 tcgatatcaa atgatgcatg ctcacgaatt tttttcacat cttcttgcgg gatttcacca
     1261 agttctgccc aagcttcatc tgctaaaatc tcgacttcga gccacgcacg gtatttgttc
     1321 tcttccgtcc agatattccc catttcaggg cgtgtataac gttctaacat gttttttctc
     1381 ctctaatccc aaatattcgt ttgtttgatt tcttccagcg tttgctggat gtcttctgtc
     1441 aagatagtga tatgtcccat cttgcgacct ttttttgctt gtttttttcc atagaaatgg
     1501 aattgccagt ctggcttatt ctcaatcaag cgatagcttt ccagcagttg ttctcccaaa
     1561 acattgacca ttactgcatt gcttatcaaa cgtacttttt ctcctaatgg ccaaccacaa
     1621 atacctcgaa tatgggcatc gaactgactc atagaacaag cttcaataga ataatgtcca
     1681 gaattgtgag gacgaggagc taattcattc acatatagcc cgcctgtttt tgttaaaaac
     1741 atttctaccc ctaaaacacc tgaaaggctc actgcttctg caatcacacg ggcaatacgc
     1801 tcagcctcct cgatcacatc ctgatcaatc gcggctggag caatcgtttc atgaagaata
     1861 ttgttgtggt ggatgttttc cacgaccgga aaagtcgtga aatctccttg accatttcct
     1921 gccaccatga tcgaaatctc tttttcaaac gggatccatg cttctaaaac acaggttcct
     1981 tctcttaaaa ggtccatggc aggagctaga tccgcccgac ttttcaaaac atactgcccc
     2041 ttgccatcat agccgcctcg agtggttttc aaaacacaag gatagccgat cccgtcaatc
     2101 gcatcttgga tatccgtcgg actgacgatc gttgcataag gagcaatcac gatgttattc
     2161 gtttccaaaa aagatttttc taacaggcgg tcttgcgtaa tcgcaagcaa gtctgtccct
     2221 tgaggaataa atgacatggg taaaatcgca tttaacgcct ctacactgac gttttcgaat
     2281 tcataggtaa tgacatctgt tcttctggca agctctttca gcgcaaaggt gtcatcatag
     2341 acactttcaa tgtgccaatc tgcaacttgt gcggctggac aatcgctcac aggatccagc
     2401 acacctactt taaagcccat ttcttttgca ctaatcgcca tcatacgacc taattgaccg
     2461 ccaccgacga tcccgatcgt tgaaccaggc aacaaaggtt taaccaagtt gatcactact
     2521 ttctataaca gttttagcca acattgaacg tctttcagcc aatttagcct ccaattctaa
     2581 atcatacatg gaaagcatct gtatcgcaag tagtccagca ttaattgctc ccgcttttcc
     2641 gattgctgta gtagctacag gtactcctcc aggcatttgg acaatcgaaa gaagcgaatc
     2701 caaaccattt aatgttcgtg attggaccgg tacaccgata accggcaaag ttgtttttga
     2761 tgcgaccata cccggcagat gcgcggctcc ccctgctcct gcaataatga ttttgattcc
     2821 tttttttcga gcgttttgtg catattcaaa catcaaatct ggggttcgat gagcagaaac
     2881 gacctttttt tcataagaca caccgaattc atccaatatt tgacatgctt ctttcattgt
     2941 ttcccaatct gaagtactcc ccataatcac agagattttt gctgtcattg tttttcctcc
     3001 cagtttttct acattcatat tttaacaatc actttttttg atgtcaaaga aatttcgaat
     3061 gttaacaaag aaaaacattt tattgttcgt gtttattaaa gataaaaaaa aggaatgtga
     3121 caaaagttag gtcacattcc tttttatgga taaacggtgt tctcctgcca actcctcggt
     3181 cacaaggaac aatattcaac aaacatgaga agcttttttt tgaaattgtt ccttgtgact
     3241 cggagatgaa cggcaggctc acgacctctt ccttcaacgg tgttcaatca gctgaccctc
     3301 ggtattagct caaacaaacg gcaaaatacg aggaactatt tttccgtttg tttctctaac
     3361 aatcaggtca aaacgctgat ttcacaacct ttcttttgct ctattacttc ttgaaggctt
     3421 cttccaatat ttcttcatct tttttcaatc cattaaagat caaattcaat atgatagccg
     3481 taatgctact cattacgatg ccatttccag tgaacatttg caatgtttgc ggcatttgac
     3541 tgaataatgt cggcgtaaca ttgaaaccca gcccaaatcc aacagaaaca gcaacaatca
     3601 atagattttt atcattgctg aaatcaactt ccaacagcat acggattcct tggactgcca
     3661 ccatcccgaa catgactaac attcctccac caagtaccgg ctcagggatg atttgtgcta
     3721 acgcaccgat tttagggaat aatcctaaaa caatcaagaa gaatgcagag aaatagatgg
     3781 gttttcttgt tttgattccc gaaagttgaa ccaatccgac attttgagaa aagccagtat
     3841 aagggaatgt attgaaaata ccgcctaaga taacggcaag tccttctgca cgatatccct
     3901 ttttcaattc ttcttctcca acatttttcc cagtaatgtc accaagtgca aaatatacac
     3961 ctgttgattc taccatgctg accatggaaa tgatgatcat tagtaaaatc gaccatacgt
     4021 cgaaagttgg tttaccaaaa taaaagggtt gtggaacatg gaacaacggc gcatctccaa
     4081 tcgcgctcag atcaatcatt cccatgaaag cagctaagat gcttcctccg actaaaccaa
     4141 tcaataccgc gattgaacga atgaaccctt ttgccagcat ttgcacgcct acaattagga
     4201 gaatcgtcac aaatgcaagt agcagatttg ttttgtcacc aaaatcagct gctgttgcat
     4261 tacctccacc cattttctcg attgctacag gaatcaaagt caaaccaata acggtgataa
     4321 ccgttcctgt tactatagga gggaatagtt ttttaatttt agaaaagaca ccagaaacta
     4381 acacaacaaa gatcccagaa gcgataatag atccgtagat agctcctact ccttcattgg
     4441 ttccgatcaa gatcagtgga gctactgctt ggatcgcaca tcctaaaacg acaggcaagc
     4501 caattccgaa aaatcggtta actgtcaatt gcaataatgt cgctaatcca cacataaaga
     4561 tatcaattga aatcaaataa gtcatctgtt cttgattaaa gcctagtcct gtaccgatca
     4621 aaagaggcac cgctactgct cctgcataca tcgctagcag atgctgcagc cctaaaaccg
     4681 cagcttttcc attttgtgtt tctttttcca cgattatgcg tcctcctcaa gaaactcaac
     4741 ttgtccattc gtcaaggaag caatacgcgc aagagaaacg actctcaatc ccatttcttc
     4801 aagtaactga cgtccatctt ggaaagactt ttcgatgacg atcccgattc cttctacttg
     4861 cgctccagct tgctggcata attcgatcaa tccttttgcc gcttgtccgt ttgccaagaa
     4921 atcgtcgata atcaatactt tgtcttctga agataaaaat ttacgagaaa tagaaatcgt
     4981 acttgtcact tgtttcgtaa aagaataaac agaagctgtc aataattctt catccatcgt
     5041 caaacttttt gcttttctag cgaagatcat tggaacatct aattctcttg cagcaaataa
     5101 agctggtgcg atcccagaag cttcaatggt cacaactttc gtgattcctt gttctttaaa
     5161 tacttctgca aaccgttttc ccatttgttc catcaataca gggtctactt ggtgagtgac
     5221 gaagctgtcg acttttaata cgccctcgcc taatatacgt ccgtcttttt gaatacgttc
     5281 aactaattct ttcactttat ttgctccttt atcatttatt aataccgaaa taaccgcttt
     5341 tgtgcaaaaa cctactatgt ttctgttaaa gaaaaacacc ctccacttta tcataagaaa
     5401 agaagggtga aatagccaat cttttactta tagtccagca tttacggtac tggctagaaa
     5461 ctgtcatcca tattatgacg atatataagt aggtatgtaa aactaataat agtgtaaatt
     5521 cattttcttg caactgtttt gcacgtgaag gcagtttgcc atatttgaat aaaaaatgtt
     5581 gtttcaaata ttgaaaacag tctaacacta ctttctgaaa ctgacaacgt ggtttactta
     5641 tttttttgta aaatctaatg atttttaatg cattattcct ttttgttcgg gtttaaatag
     5701 ttatttttat tttctcctat ctaaaaaagc agcccacata aaaagaaggc atgacaaaaa
     5761 gcatgtcata gccccttttt ccgaataaat agtgttcaaa agacagccta tcggcaataa
     5821 ggcaagattt tataaactac gggaagtaat ttccgtaaat ctattcttat tcttttaagc
     5881 tgacaagctc gtcaatcact actgcttttc ctgaagcaat agaatgattt gctgcgattc
     5941 ctgtcaaaat cgacaaaata ccatctacgt gagttgctgc ccgattccac ggatcttctt
     6001 ctttttcttc accaaataga tcatctaata aacgcggatc tcctccgcca tgtccgcccg
     6061 cttcttgttc gacgattacc tcatatggct caccgaacat cggatagaca tggatggact
     6121 gaagaacggc tgctccttca tcctttttat ctccacctgc attgatgtaa gacttttcga
     6181 tcactttcat ttcgatccgt cctttgctgc cattgaaaga aacattgaat ccttcccacg
     6241 gcatatacgc gtttagcgaa tacgtcaaaa tcgcaccagt ttcatatttg accgttacgc
     6301 ctagtgtatc ttcaatagag atatcatcgg aaaatacact gcggtcacga atatatccac
     6361 tgtctttttc tgcttctaaa tacatttctc gcaattcctc attggctgac aggtctatag
     6421 caaatggatc agctttagct agttcgcttc catagcatcg actgtaaaat atttctactc
     6481 cgcgtttctc cgcattatgc atcccataaa agtttaatcc accgaatgca aatacctttt
     6541 ggggacggct gttcaaccaa aaattcacta gatcaaaatg atgggtagat ttatgaacaa
     6601 gaagcccgcc gctattgtgc ttatttcgat gccatcttct gaaataatct gccccgtgtt
     6661 gcgtatttaa cagccattcg aagtggatag aaaacacatc accaatcaca tcactttcta
     6721 tcagttcctt tatcttcgtg tgatacggtg cataacgata attaaaagtg actcgcaggc
     6781 ttcttcccgt ctgtttttgg accgttatta tttcttgagc tttttcctca tcgatagtca
     6841 gaggtttttc gcaaataaca tcatatcctt ttttcattgc acgaatgata taatcatgat
     6901 gcgttcgatc tactgacgta ataatgactt cagccggccg tacgtcatcc agtagttgct
     6961 caaattgtga tggcatacat gtctttactt ttttctgtcc atacttgttc acaagtatcc
     7021 gatttgcata atccatcctt gtttgagata catcacaaaa accgactagt tcacaagttt
     7081 gatgatacgt agaggcaatt gcctcataaa aaaaacgcgc tctcccacca agtccgactt
     7141 gtacatattt tttcttcatt taaagtcatt ctccttttct tttaagcaaa atactgtgac
     7201 aaatggcagc cctggataca gtaatgcagt gagtaaaaaa tcatgctgta tacaccagaa
     7261 cagtccatac aaaaggcata aataccacag aaaccccttg acgttttttg acaacagcca
     7321 gatattcctt ttgatcaaat aaacaagact atctgcttta tcttctttgc agatatcaat
     7381 tagcgtacta aaaaaactat gggatacaaa aagcaacaga cacaatagaa aaggaatcag
     7441 ataccaagct ccaatctttc ccactaataa ataccaatca actgcaaaaa taatgacaaa
     7501 cagctgaaac aacgtaatga tgaaacacat ccgtttgatt tttcctttcc tccaacatac
     7561 ctttttttct gccaagtaat ggacagatag ataataagaa ggatacatca tcaaaaaata
     7621 aatcagatag acaacacgag cagacagcac taatcctaaa gtgaacaaac atcctagaac
     7681 taaagcgaaa agagcattac tgataagact ggttaaaaaa atccgacaga catagtagtt
     7741 gagcttgtaa agaaacatct cttctattgg ctttttcata ttcagccctt cagaccgctt
     7801 gtactgatcc cctggacaag atatttattg aagaataaga aaatcagaaa aaccggtagc
     7861 agcgacaacg tagacatcgc aaacatactc gcgtaagtcg tactagatcc agcgtctgca
     7921 aataatttga tggcaatcga taccgtgtaa gatctcggct tgtttaaata aatcaatggt
     7981 cccatgaagt cgtcccattt ccaataaaac tggatgatga tcgtcgtgat gatcgaaggt
     8041 ttcagcaaag gaaaaataat tttggtaaag acactatact tgctgcatcc gtcaataatc
     8101 gctgattcat ctaactcttt ggggatgctg accatgaatt gcatgatctg gtaaatgaaa
     8161 aatgcttgtc caaaaaaatg tggtaaaatc agcggcacat acgtcccaac caaattcagc
     8221 cgattgtaaa taataaactg cggaatcaaa atcacttgtg caggaatcat catcgttact
     8281 aacatgacga cgaaccagaa tctttttcct ttgaaatcga ttctcgataa tgcgtaagca
     8341 atacaagtcg agctcagaac tgtccctatc gtcgaaactg cagagatgac aaacgaattc
     8401 ctaaagaaag taaaaaatga aattccagca aaccccgccc atccttttgc gtaattttcc
     8461 cattgaaaag tctctggaat caaattcagc gaaccactca taatctcttg atccgtttta
     8521 aaagaacctg cgatcatcca taagaccgga taaatcatga ctagacctaa tagcaaaata
     8581 aaaccgtatt gaaaccattt cgttactgta ttattcattt cttcctcctc tcattcacac
     8641 aacgacccat ttttttgatg atttgaaaat cagaaaggtg acagctccca taatcaacag
     8701 catgatccac gaaatagcac tagcataccc catagaatta aaagaaaaag cttggttata
     8761 cacataaaga gcataaaagt tcgtggcatc attcggacca ccttgggtaa tgataaatgc
     8821 ctgcgtaaaa gtcataaacg ccgcgattgt ctgcataacc aagttataca aaatgatcgg
     8881 cgaaagaaga ggtaacgtga tcttgtaaaa cgtctgccat ttgttcgctc catcgatctc
     8941 tgccgcttca taatacgagc ctgggatctc tttgatacct gcagcaaaga tgatcattgc
     9001 cgaaccgaat tgccaagcag acatcaaaac aagagggatc atgaccagtt tttgatcgct
     9061 aaaccaggaa agcgcaggtg cgcctaatcg ttccaggaaa atattcacta acccgttttt
     9121 agaaaacaac tcccgccaaa ctagagcgat agaaatactg ccaccgatca aactaggcac
     9181 ataatacaaa gaacgaaaga aagtcgttcc tttgatattc ctcgttaaaa gataagcaat
     9241 caatagacca aagatcagtt tcacaggtac agatgcaaat acatagagaa aagtgactcg
     9301 gatggagttc aaaaaacggg catcactcag catctttata taattatcca agccgatcca
     9361 tgagactgga tttcccaaac gataatcggt gaacgaataa taaagtgaca agatcatcgg
     9421 tatcactgtt atcaatagaa atcctaggat gaatggagaa gcaaatacgt aaccgaccac
     9481 attgtcttga tccaagaacc gttttagttt ttcttttttc atgccttcct cctgctgcta
     9541 tcctagaatg tttgacgcaa actcataaag actctctgct gcttcttctg ctgttacttc
     9601 ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg gattcaaagc
     9661 actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta aataatcaaa
     9721 tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat tcgacatgat
     9781 tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt gaaagaaatc
     9841 aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca gcatttgcga
     9901 agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat taatcaactg
     9961 aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac ttgatagaaa
    10021 caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg tacttgcagc
    10081 tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca gctggaaaaa
    10141 atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt cccgattata
    10201 aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta taaaatcttc
    10261 gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga tttgttcaaa
    10321 ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa atatattttt
    10381 attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt gcccgtcgta
    10441 agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc cttgctccac
    10501 atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt tcagttggaa
    10561 aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat atccttcata
    10621 atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa gctcgattac
    10681 ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca gcgtgatttt
    10741 tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta aagctaagat
    10801 gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt ttcaatatac
    10861 tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat aaaaaccaag
    10921 acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa aattagatag
    10981 tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga ggataattac
    11041 gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta aataagcatg
    11101 cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc tgtatgaaat
    11161 taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat tggtcagttc
    11221 ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt gcctttttta
    11281 gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca tttttttgtt
    11341 agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag gtaaaaaaac
    11401 aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca aaagacgagg
    11461 aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca tgattctttt
    11521 tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta cttggtggcg
    11581 tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct tccgttggac
    11641 cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt actgcttctg
    11701 cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac ttttcatcta
    11761 tttctgtata tttcgacgtt accgtactga taactggaat atcaactact cgacaaactt
    11821 cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa ccttgcgctt
    11881 ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca cctacaccgg
    11941 agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga tgaggtgtaa
    12001 aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca atatccgtag
    12061 taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct tctaaaatga
    12121 tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc gtaaattgtt
    12181 ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt gatacgtctc
    12241 gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg catcttgcaa
    12301 tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca gcgcaggatt
    12361 ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt cccatgaatc
    12421 atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt cctttgtttc
    12481 caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat tgtatttgct
    12541 agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa tgatactagg
    12601 aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt caataaattg
    12661 ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag acagattctt
    12721 caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg ctggccaacg
    12781 gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt cctacttttc
    12841 atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat actcagcgtt
    12901 ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta tatcttcgtg
    12961 tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga tccatttagc
    13021 tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt tgtaccagtt
    13081 ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg gctccgtaat
    13141 cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac tcatcacatc
    13201 ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag ctgagaccat
    13261 cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt ctggagcaat
    13321 gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact catcaatttc
    13381 tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg ttttaacatc
    13441 tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc tgctggttgt
    13501 ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga ctgcttgatt
    13561 gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg ctgcgatcat
    13621 aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt cgttgttctt
    13681 cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc acccaatcga
    13741 gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga cgaaaattca
    13801 aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca aggtatctac
    13861 cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg ctgctaagcc
    13921 atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg tttcgttttt
    13981 cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca taatcagtga
    14041 aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca tctttccatt
    14101 tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt tcagtttcaa
    14161 actgttctaa taatacactc ataataagat caatatatac ttgatgcacg tttatcccct
    14221 ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg ctgctttcat
    14281 tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt ataataatag
    14341 acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa atatcaatgg
    14401 ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa tcaagagacg
    14461 agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt cgctcaccgt
    14521 tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc tgaaatcgac
    14581 atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc agcattggaa
    14641 gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga actggctgac
    14701 gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac aatcttcaac
    14761 atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg ggctttaaaa
    14821 atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg gttcttcaat
    14881 ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc tttcgctcgt
    14941 ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt tgaaacaggc
    15001 gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc tgctgtcagc
    15061 ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa cggttatgtg
    15121 acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga tggtacaaag
    15181 gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa gaatatggta
    15241 cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga tgtcatcgaa
    15301 atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt tgtgaaaaag
    15361 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
    15421 ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag aggttgtgag
    15481 cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt
    15541 gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag aagaggttgt
    15601 gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt tggatttttt
    15661 gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa aactggaaca
    15721 attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc cgatacaata
    15781 cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct gaaaatgttt
    15841 ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt tgctggattc
    15901 tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc atagagaagt
    15961 aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct ctggcatctt
    16021 cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca tcttctttat
    16081 tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct ttttcgatct
    16141 gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt gcaagcaaag
    16201 caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc caagacatgt
    16261 tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct tcggttaatt
    16321 tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca aaaatagtat
    16381 acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag gctgcgattc
    16441 gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat aatgcaatga
    16501 tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc gaaaagaaga
    16561 cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc cttttttatt
    16621 ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt atgatagtaa
    16681 aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt atgcgtacga
    16741 tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact caacgaggaa
    16801 tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg actacaaccg
    16861 tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta gctatcattt
    16921 atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc ttgcttccta
    16981 ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc ggtatcattt
    17041 ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa tccgctttgt
    17101 atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc ggtatcaatt
    17161 tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat ttggtcgaac
    17221 tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct aagatccaaa
    17281 aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa aatggagaga
    17341 agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact actttattct
    17401 tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac ttggctagta
    17461 gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt tgtcgctttt
    17521 agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg gttcgctcca
    17581 ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat atttcctagc
    17641 ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact caagacagct
    17701 gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc ttctgctttt
    17761 ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt attggcttcc
    17821 gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac ttctttgact
    17881 ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt tttcttcact
    17941 tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc gatttgtttt
    18001 tgacgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg tgctcgggca
    18061 ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc ttgtttgtag
    18121 gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg tcgctgtaat
    18181 tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc ttttagagct
    18241 tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt ccctaaagag
    18301 tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat gatcaacccc
    18361 atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt atcgcgattt
    18421 tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc ttctaatact
    18481 tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc tgctgctgta
    18541 gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca catcaccggt
    18601 acgccttgtt ccgtatacac ttcaggggtg gagacgtcca atttactcga taatagactg
    18661 atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg cacaattttg
    18721 attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact tccactgatg
    18781 atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat cagcaacatc
    18841 agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc catttaacac
    18901 acgctccttt aagcttttat catgctttca taaggcatga cataacagat tctttccttg
    18961 atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg gctgtaaaaa
    19021 caagctggac gactaatgga accggtaaca cttttcagct gaatttctcc catacctcgt
    19081 acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc agaagacgac
    19141 atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa aaataccaac
    19201 actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc tgttaaacgc
    19261 tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag catcggatca
    19321 agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc caatcctgca
    19381 caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat catatacacg
    19441 cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata gcttctttta
    19501 ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt tgttaaattt
    19561 tgttttcatg ttaaaatttt cacaataaat tctttcgagg tagcctatgt ttcctttttt
    19621 taagaaaaaa c
//
LOCUS       DAYYNG010000042        19464 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000042 DAYYNG010000000
VERSION     DAYYNG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.042"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..3607
                     /locus_tag="ACHBFA_001201"
     CDS             80..3607
                     /locus_tag="ACHBFA_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9394707.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRAITNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3604..7326
                     /gene="addA"
                     /locus_tag="ACHBFA_001202"
     CDS             3604..7326
                     /gene="addA"
                     /locus_tag="ACHBFA_001202"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9394708.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7395..7736
                     /locus_tag="ACHBFA_001203"
     CDS             7395..7736
                     /locus_tag="ACHBFA_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9394709.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8045..9088
                     /gene="pheS"
                     /locus_tag="ACHBFA_001204"
     CDS             8045..9088
                     /gene="pheS"
                     /locus_tag="ACHBFA_001204"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9394710.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9093..11513
                     /gene="pheT"
                     /locus_tag="ACHBFA_001205"
     CDS             9093..11513
                     /gene="pheT"
                     /locus_tag="ACHBFA_001205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9394711.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11683..12693)
                     /gene="trpS"
                     /locus_tag="ACHBFA_001206"
     CDS             complement(11683..12693)
                     /gene="trpS"
                     /locus_tag="ACHBFA_001206"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9394712.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12947..13684
                     /locus_tag="ACHBFA_001207"
     CDS             12947..13684
                     /locus_tag="ACHBFA_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9394713.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13698..14513
                     /locus_tag="ACHBFA_001208"
     CDS             13698..14513
                     /locus_tag="ACHBFA_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9394714.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14526..15173
                     /locus_tag="ACHBFA_001209"
     CDS             14526..15173
                     /locus_tag="ACHBFA_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9394715.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15189..15845
                     /locus_tag="ACHBFA_001210"
     CDS             15189..15845
                     /locus_tag="ACHBFA_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9394716.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16007..16828
                     /gene="racE"
                     /locus_tag="ACHBFA_001211"
     CDS             16007..16828
                     /gene="racE"
                     /locus_tag="ACHBFA_001211"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9394717.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16831..18186
                     /gene="rph"
                     /locus_tag="ACHBFA_001212"
     CDS             16831..18186
                     /gene="rph"
                     /locus_tag="ACHBFA_001212"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM9394718.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18187..18705
                     /locus_tag="ACHBFA_001213"
     CDS             18187..18705
                     /locus_tag="ACHBFA_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9394719.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18812..19312
                     /gene="cbpB"
                     /locus_tag="ACHBFA_001214"
     CDS             18812..19312
                     /gene="cbpB"
                     /locus_tag="ACHBFA_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9394720.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3342 c         4180 g         5401 t
ORIGIN      
        1 ttgaagaata tagaacaaat gttcagtatt ttttgatata ataaaggagc accgtatcat
       61 gtgttgaagg aggaaaagca tgtctttaca atttattctt ggaaacggga aaaaagatca
      121 ccgtcaagtc ttgatcgacg aagcatccgc ttggctcgac caggatgcaa gtaaccaagt
      181 atttttcctt gttcccaact acagcaaatt cgaacaggaa caagaaattt tagcggagat
      241 gcgaagaaga agcggaaaaa aaactttcag cacaacaaac gttcaagtat ttagttttta
      301 tcgtctggct tggtatttgc tgcaacagac aactcttctt acaggaaatg aattatcaga
      361 atcaggatct gcaatgatct tgcgacagat tttagaaaaa aatgaggaag aattaacaat
      421 ctttcgcgga gaaatcaata aaagagggtt tattcgtcaa ttacaagaac tctacagtga
      481 attgcgtaca ggaaatattc aaccagaaga cctgatgctt ttatttggtg attcacctaa
      541 tgcagaagac cagcaactta aaatgaaaga cttgaagctg attttttcag cttatgacag
      601 cgaattgact caacgtgcaa taacaaatga agatgcacta tctatactag caaattatat
      661 gcagacacaa gatttcaaca acgtcaaatt tattgtcagt ggtttttctc gaatcaatgg
      721 tcaagagtat caattactgc aggcaatgat ggaaaagggg caattggttg tcgatttatt
      781 gctggatcgt ccttatacag cggatcttcc agaagtactt acgctctttc atgagactgg
      841 aaagctttat ttccgtcttt ttcaaagtgc aagagaacgg aaagtcccta tattagtgga
      901 ccgatttgca ccagagcaga ctcgtccgac agagcttcaa ctgctgcaaa caatgtggga
      961 agagacatct agccaaaaga aagcggcagt acacaaaata gggggacaag agcatctcca
     1021 tttgtgggaa gctgaaaatc caactgaaga agtccgacaa ttagctgttg aaatcagacg
     1081 actagtcagt gaagaaaact accgttatcg tgatatccaa gtgctgacaa aaaatatgtc
     1141 tttatatgga aatatccttc ggccgatctt tcaagaaatg gcaattcctt tttatatcga
     1201 tgaagaacaa atgatggaaa atcatccgct gatcgaatgg atcaacagtc tgttcgctct
     1261 tgaccgctat tcttatcggc taagtgatat tatgcgtttt ttcaaaactg aattatttct
     1321 gtcgacggaa gcagaaaccg aacttgatga atggaaacaa gagcgagatg aattcaggaa
     1381 aaaactggac atcactgaaa atgtagcact tgcttacaat tatcaaggaa cttattggac
     1441 tcgtgaaaaa gactggcagt ttgtggcgta tgattttgaa gcagaacaga ttgaagacgt
     1501 acagtatttg gaagaacagt caaatacgat tagacgatta tttcagaaag ctgtaccagt
     1561 atttttgaaa aaaataaaga aaagcaaaac aggacaagag gccgcaaatg ttttttatca
     1621 atttttgatc caaagtggcg tacagcaaca attattgttt tggcgtaatc aggaagtaga
     1681 cagaggaaac ttagaagcgg caagaaacca tgagcagaca tggggagcgc tgattgatct
     1741 gttggatgaa tatgtagtaa tttatggtga atcggctttt gactggtcac tgttccaaga
     1801 aatcattaca agtggtctag aaaatctaag ttatggtaaa atccctaccg caatcgacca
     1861 agtgcgaatc aatcgtttgg aattagttcg accaaatcaa gctgccgtca cttttgcatt
     1921 agggttgaat gatcaagtgt ttccagaccg aaaagagaca aaaggattgt tgtccagtga
     1981 agaacgagaa gtattaaatc aggccttgcc agatggacag tttttattcg acccatcaaa
     2041 ggaaagtatc tcttttgagc ctttccaagc ctatcttgtt tttctttccg gaacagatcg
     2101 attatatctt agttatgcac aaagctatga cacagatggt tcgctgaaga tgtctcctta
     2161 tttgcgtaga atcacagaat atttatctgt tcccgtcgag agaaaagagc atttgacgat
     2221 cgaaagcgac cctaattgtt atgtagggac ttacagaagc ggaatcaata cagtcaatag
     2281 gatttatcga ttagctttag atgaaaaaaa acctgtacca aagcactggc aaaagctcaa
     2341 agaattagta ctgaattcgt catggcgaaa ttttgcttta cagatttttg aaagtcagga
     2401 acatttaaat gttcctgttt cgatgccaga aaaaatggcg gaagagcttt atggaaaaga
     2461 gatttatact tcaatttcac gtatggagaa tttttataat tgtgaataca aatacttcat
     2521 gcaatttggt ttgaaattga aagaacgaac tgtttatggt ctaactccag cagcaactgg
     2581 tgatttttat catgaatcat tggatcgttt cttcaagcta ctgttttcaa accaattatc
     2641 cttagtgaat atgtccgatg cgcagcgtag agactttact gaaaaagtgc tgcaggaagt
     2701 attcggagaa cttcggtttg agatattgga tagttctgca agaatgaact atatccgcta
     2761 ccagttagga cagacgattc aaaaagtgac atgggctttg caaaaacaga gcaaaaatac
     2821 aggcatgcat cctcttcaga cagagattct atttggtcag attgctggag caaaaggtat
     2881 cacaggtctg gaattaccgt tgagtaatgg tggaaaactc catgttcgag ggaaaatcga
     2941 tcgaatcgat gtagcttctg aacaagaaga tacttggcta agcgtcgttg attacaaatc
     3001 tagcggacgt tcattcgatg tcacagaagc ttattatggc atggctatgc aattattgac
     3061 gtatctggac gtagcattga tggatgcggt acgtttgact ggaaaggcgg ctgttaaacc
     3121 agcaggatca tattatttac atgttcataa tcctgtattg acggagacag atgatgtaga
     3181 gaagaataca ttgaaaaaat ttagttatga tggcgttttt gtcgatgatc ccgctctttt
     3241 agaagtacta gatcattcac tggaagcaaa agaacaatca ctgatctatc cagtgaaaaa
     3301 aaatgcgaaa gatgaatacc aaaaaggtag tttcagtaaa gacaaatttt tcacggaaga
     3361 agaactgcaa ctattcatgg cgcataatcg ggaaaatatg cggttagctg gagatgcgat
     3421 tacttccgga gaaatcaaac taaatccagc ttataaagat aaacagcgga ttgcctgcga
     3481 attttgtcca ttcagaagca tctgtacgtt tgatgtgatg ctgaaagaaa ataattacca
     3541 ccgtctggag aaaatagata aggaagaagc actgcgtaga atattgaaaa ggagtgaaga
     3601 agagtgaatc aaattccttt aaaaccgcaa aatgaacgat tcacagatga tcagtggcaa
     3661 gcgatttttg atcaaggaga taatctatta gtctctgcgt ctgccggatc aggtaaaact
     3721 acagtgcttg ttcgaagagt gattgaaaaa ctaaaaatgg gttttgatat cgatgaatta
     3781 ttgattgtga catttactga agctgccgca agagaaatga aagaaagaat tcaagaggca
     3841 ttacaagaat ccgtcaatag cgaaagtgac cctgtacgta gacagcattt tactaagcag
     3901 ttagttttat tgcccacagc gaatatctca accttacatg ctttttgcct gacagttatt
     3961 cgacgttatt attacttaat tgatattgat ccagtctttc gcatgttaac agatgaaaca
     4021 gaaacgatct tgatgaagga ggatgtgtgg gacgagctga gagaagcact ttatgcagaa
     4081 aatgatgaac gatttttcca attaacaatg aatttttcta atgatcgttc agatgacggc
     4141 ttgacgaatc tggtgttttc tctttatgag tttgcccgtg ccaaccctga cccccaaaaa
     4201 tggctagaac agttaagcga taattatcgt ttgcctgagg ggctagcgaa atctcgatta
     4261 tatcaaaaac aaatccgtcc tttagttttg gcagatatat accaatgtgt acagttatat
     4321 gaacagatga ctcagttagc acaaggtgaa ggattagaaa aaatgcatga gcaagtagct
     4381 ggagaacaac agcagatcaa gaatatctat gaagcgttct cgcaagatcg gttagaagaa
     4441 gcttatgctg gtttggaaca actaactttt tcaactttta agagcagtcg gaaagcagaa
     4501 ttgaaagaaa tttccaatga agtcaagggc atgagggata aagcaaaaaa actgattcag
     4561 cagataagta aaagttattt tcctgtttcc ccaagccaaa tggaagaatt aacggacaaa
     4621 gcactaccgt tagtcgaaga aatgacaaaa gtcacgcaaa gcttcatgga cggtttcagt
     4681 atgcgtaaac gggaaaaagg tgtgctggat ttcaatgatc tggaacattt agcgttgcag
     4741 attctcacag aaaaaacgaa agatgtatgg ctgccaagtg aagcttcaaa acattatcga
     4801 aaaaaattca aagaagtaat ggtagatgaa tatcaagacg tgaaccaatt acaagaagcc
     4861 attttatact ggctaagaga acccgatgat acaaaaggaa atatgttcat ggttggggat
     4921 gtcaaacaat ccatctattc tttccgttta gcagatccta gtttatttat cgggaaatac
     4981 gagaatttct caaagaaaga aggcggacgt cgaatcgttt tggctgagaa cttccgctct
     5041 agaaaagaag tcctttcttt tacgaatctt atttttgaac aactaatgga tccagcagtg
     5101 ggtcaaatca attatgacga agcagccaaa ctgatccaag gattttcaga ctttccagag
     5161 aatgaacagt ttgagccgga aatcatgatc tatgaaaaag aacaggaaga atcagagatt
     5221 gagattccga cggatgatat actggaagac aaaacagaag gcgaactttt tatgacagga
     5281 ctaaaaattc gtcaattaat tgattcctcc tttatgattt atgataaaaa atcaaaaaaa
     5341 agtcgtccga tagagtacaa agacattgtt ctcttgacac ctacaaagaa gaacaactta
     5401 acgattttag agattttcaa aacattggat attccattag agatgaatga tgctcaaaac
     5461 tattttcaag cgacagaaat tcgcacgatg atctcgctgc tacaattaat cgataatccc
     5521 tatcaagata ttccgcttgc ggctgtattg cgctcaccga tcgtgggtct gattgaacca
     5581 gaattagcca gcattcgatt agctaaccgt gcacatactt actatgatgc tgtgcttgct
     5641 tatcaagcat caaacgaaga tgaattagca gctaagctgg aacattttgg caagcaacta
     5701 gagcattggc gagaacttgc tcgccgttct tcgatcactg acttgttatg ggatatctat
     5761 tatgagacgg gatatctaga gtatgtggtc ggtctgccag caggggcaca gagacaagcc
     5821 aatctttatg cattggttga tcgtgccaaa gcttacgagc aaagttcttt cagaggatta
     5881 taccagttcg tgcgtttcat tgaaaaaatg caagaaaaag ataaagactt agcagaacca
     5941 gtcatttcaa tagaagataa tgctgttcgc gttatgacga tccatgcaag taaaggcttg
     6001 gaattcccag ttgtattttt gttagatatg acaaaggagt tcaacttaca agacttgcgc
     6061 aatcggtatg cttttgaaga aaaacttgga gcaggaattc gctatatgga tcctgaaaca
     6121 cgagtgttat atgatacgtt accgtttcaa gccatcaaat tggcaaagca aaacaaattg
     6181 ttgtcggaag aaatgcgtaa attgtatgta ggattgactc gggcagaaca aaagcttttc
     6241 attgtcggtt cctataaaaa taaagagcaa atgatccaaa cgtggtctga agcagctgat
     6301 catgaggagt tagtttttga tcctgcgcta cgtttgaagg gcagaagcag cttgatgaat
     6361 tggattggtt acggattgat ccgtcatcct gaaatgcaga aatacttaga ggaagaaatt
     6421 tcgacttctc ttcttcaaca ttcaaatgca cagttttcta tttcatggat gaatcagcaa
     6481 tcgatcattg aacaaagaca gttgcttgct gaaaaagaac tggttaactt agatcagcag
     6541 atgaaagaag atgaaacttt attagcagat tcgttacaaa aacgtctggc atatgaatat
     6601 ccttatcaag cttcttcaca aaccacaagt tatcaatctg tatctgaaat caaacgatta
     6661 ttcgaagatc cagatgatac acaagaatca cgtttgactc tggaaagcag tcagaacaaa
     6721 gcagcatccc gtcaatttcg ttatacgcaa gaacaactag cagaaccaaa atttttgcaa
     6781 aaagatcggc aagtgtcagc agctgctgtt ggaactgcta cgcatgcttt gttacaatta
     6841 ctgccattag agatgccaac tacagagagt atccatcaaa aattacagga attggtaagg
     6901 aaacggttag tagacgaaaa agtcgcgaaa aaagtagatg tttcttctat tatatggttc
     6961 tttcaaacag aacttggtca acagctgatt gccaacaaag aaaacgtaaa acgagaacaa
     7021 cctttttcca tgctgcttcc tgctgacgaa gtatttcaag actatccaaa tcaagaagat
     7081 gaactattaa tccacggaat cgtcgatgga tacctagaag aaaaagatca tctcaatatc
     7141 tatgatttta aaacagattt tattttacat ccggatgatc cagcagaaat agatgcaatc
     7201 gtccaaaaat atcaaggaca gctaagactt taccaacaag caatgtcaga agctttgaat
     7261 aagccagtag aaaatgtttt cctaattttg cttcgagtga aacaaattat aaacattaac
     7321 aaataattat aattgagcaa acttgactta tgttttgtaa ggattatgct atactctggg
     7381 aagaggtgag tatgatggag ctaacacaga cagaattcgt tttatgccct aaatttgaaa
     7441 aaacattctc tattttagga aaaaaatgga acggattgat tatcgatgta ctactcgaaa
     7501 acggtccgca acgctttgtt gagcttgcgg caaaaatccc gatggtcagt gatcgagtat
     7561 tagtcgaacg cttgaaggaa ttagaaaaag aaggaatagt cactcgaaac tttgcctgta
     7621 gcgaaaagag ccgttcagaa tatctcttga ctgaaaaagg agcagacttg caaaaagcga
     7681 tgcgtcaaat acaagtttgg agcgaaaaat ggatcacaga ggaagaatgc ggttgatttt
     7741 gtaaaaaaaa tcttgtaaac tatagtccat acgaataagt aaagaacgat gacgaaaaag
     7801 agtagtttgg ctaaaccagt tcagggagat ctgtcgcaga ctgaaagcag atcctgtgtt
     7861 tgaaaaatga agttcacttt ccgagttcgt ctgataaaat cagaccggca attgtcgtta
     7921 acgaagtaag agtgcggtgt aagtcattta tccgcaaatt aggatggtaa cgcgaagacc
     7981 tcgttcctta acaggaatga ggtctttttg ttttgtattt ggaatttaaa ggaggaacaa
     8041 gcacatgtct ctacaagcac aattagagag cttgaaagaa gcaacgctcg tcaaaatcac
     8101 agcggcacaa gatttaaatg aattaaatca aatacgtgtt gaaacgttag gaaaaaaagg
     8161 accgatcaca gaagtattac gtgggatgaa agatctaagt ccagaagagc gaccaaaagt
     8221 cggaagtttc gcaaatgaga tccgagatct tttgacagaa gagatcgagc gaaaaaaaca
     8281 agtattagaa gaagaagcgt taaatgcagc tttggcaaaa gaaacaatag acgtgacact
     8341 acctggtaga aaagtaaaac atggtacacg tcatatcgtg acacaaatca tggaagaaat
     8401 tgaagatatt tttgtgggta tgggctatca gatcgtagaa ggatatgaag tagaatctga
     8461 tcattacaat ttcgaacgga tgaatctgcc aaaagatcat ccagcaaggg acatgcaaga
     8521 tactttctat atttcagacg agatattgat tcggacacat acttcaccag tccaagcacg
     8581 gacaatggaa aaacatgatt tctctaaggg tgctctacga atgatctcgc cagggaaagt
     8641 cttccgcaga gatacagatg atgcgaccca cagtcatcag ttccatcaaa ttgaagggct
     8701 agttgttgat aaaaacatca caatgggcga ccttaaagga acattagaag tcgtaatgaa
     8761 aaaaatgttt ggggaagatc gtgaaattcg tttgcgtcca agttatttcc catttacgga
     8821 accatcagta gaagtagatg tcagctgttt caaatgtggt ggtgccggtt gtaacgtatg
     8881 taaatacact ggatggatcg aaattttagg agctggcatg gtacacccga atgtgttgaa
     8941 gatgtcagga atcgatccag aagaatattc aggctttgct ttcggccttg gaccagaccg
     9001 cgtagctatg ttgcggtatg gagtgaatga tatccgtaat ttctatcaaa atgacttacg
     9061 tttcttaagt cagttcaagg gggagtaaga aaatgttagt ttcatataaa tggctcaatc
     9121 gttatgtaga tttatcgaat gtaacaccaa aagaattagc ggataagatg tctgtgacag
     9181 gaatcgaagt agagggagta acggttccag aagaaggctt gaaaaaaatc gttgtcggtg
     9241 aagtcaagga atgcgtacca catccagatt ctgatcacct ttctatttgt caagtcgata
     9301 ttggcgaaga cgaactgtct caaatcgttt gtggagcgcc taatgtgaag gttggaatca
     9361 aagtgattgt tgcacttcct aactcacgta tcgctggtaa tgtaaaaatc aaaaaaggaa
     9421 aaatgcgtgg ccaagtatca aatggcatga tttgctcatt acaagaaatt ggctattcag
     9481 atagtgttat accgaaagaa tacgctgaag gtatctatta tatgccacaa gaagctgtaa
     9541 atggcgatcc agtttttcct tatttggata tggatgattc gattattgaa ttatctatca
     9601 caccgaatcg tgcggatgca ttgtcaatgc gtggcgtagc ttatgaagtt ggggcaattt
     9661 atcgtcagac tcctacattc gatgacgaaa agctggtaga atcctcacgt cctgctagcg
     9721 ataaaattca agtggaagtt gaagataaag aagcagttcc tgcttatcaa atcagaatca
     9781 ttgaaggagt aagaatccag ccaagtcctc aatggctgca aaatcttttg atgaacgaag
     9841 gcattcgtcc aatcaacaat gtagtagatg tgacgaacta tattttgctt ttattcggac
     9901 aaccactaca tgcatttgac tacgacaaat tgaaatcaaa agagatcttt gtccgccgtg
     9961 caaaagaaaa tgaaacatta gtaacactag atggagaatc tcgggaattg acaactgaca
    10021 atctcgtcat tactaacggc aaagaaccag ttgctttagc tggtgtcatg ggagggttag
    10081 actcagagat cacgaatgaa acaacaacag ttgcactaga atctgcttta tttgatccaa
    10141 tttcgattcg tcgtacagca aaacaattta atttgcgtag tgaatcctct gcacgttttg
    10201 aaaaaggaat caaccatgcg actgttggag aagccagtga ggttgctgca gcaatgatcg
    10261 tttctcttgc gggtggggaa gttctacaag gcgcggtcaa aggctcagag atcaaagcag
    10321 aagacgtatc agtgaagatt tcacttgaac gcatcaatca atatttagga acatctcttg
    10381 tcgttaaaga agtaaatgag atttttgaag cacttggatt tagctatgaa gaaaaggagt
    10441 atgtttattc cgtagtagta ccaccaagac gctgggatat ccaaatcgaa gcagatatta
    10501 tcgaagaagt tgctcgtatt tatggatacg accagttgcc atctacactt ccaagtggag
    10561 aaacggttgc tggaagcttg tcaaaagaac aagaaactac tcgtaaaatt cgtacaatct
    10621 ttgaaggaag cggattgagc gaagcaatca gttatgcatt gacaacagaa gaaaaatcaa
    10681 gacagtttgt tataaatgaa agtaatatta cacgtttgga ctggccaatg agcgaagaac
    10741 gttctgtatt acgaatgaat ttgatcagcg gactattaga tgatatcagt tataacactg
    10801 cccgaaaaaa tacagagatt gctttatatg aaattggtcg ggtgttcttc caagaaaatg
    10861 atccacgtac acatctgccg caagaagtaa aacatgcagc aatcgctatt tctggtattt
    10921 gggaagaaaa agattggcag acgaaagctc aaaatgtaga ctttttcacc atcaagggtc
    10981 ttttagaaaa cttatttgaa caactaggca tcactgaaga tgtccattat caaacagtga
    11041 cagaaatgaa ggaaatgcat cctggaagaa ctgctgcgat ttaccttggc gaaaaattta
    11101 ttggttttgt cggtcaagtg catccaacga ttacaaaaag ttatgatatt ccagaaacat
    11161 atgttgctga gtttgattta tctgctatag tggaagctgc tcaaaaaggg atcgttttcg
    11221 aagcagtgac aaaattccca gcagtcagcc gggacatcgc tttgcttgta aaagaaactg
    11281 tgacaaatca ggaattaaca gaagttatcc gttctgctgc cggaagattt ttaacagata
    11341 tccaattatt tgatgtctat caaggagaaa acattgaaaa aggacataaa tccatggcgt
    11401 atagcctgac attcgtcaac ccagaagcaa cattgacaga tgaagaaatc aataaagcaa
    11461 tggaaaaagt cgaaaaagcg ttggtagaaa acctagaggc aagtattcgc taagaaggaa
    11521 aatatagtaa gtaacgatca agtagctttt gaagaccata catttgaaaa aaagatgtga
    11581 cacaacttcg tcacatctct ttttttgatt ttaaaaaagg aatagcacag agacgagata
    11641 aaaaaacagg cctttacagc ctgctttttt agagtaaatg aattaatgga agtaattgat
    11701 tcccattgct gattttactt cagaaagtgt ttgtgcagct actttagcag cttcttcact
    11761 acctttacgt aagatttcca taacagctga aggatctttt tccaattctt ctcggcgcgc
    11821 acggatcggt gcaaattcag cttccaatac atcaatgagg tatcgtttga tctttacatc
    11881 accaagtccg ccgcggcggt aatgagcttt catttcttca attgcttctt tatctgttcc
    11941 gaatacatcc aagtaggtga agaccatatt cccttctact tgtccaggat cttgtacatg
    12001 gatgtggttt ggatcagtat acatactcat gacttttttc tgtaagacat cggcaggatc
    12061 ggagatataa ataccgtttc ctaacgattt actcattttg ccatttccat cgattccagg
    12121 taatctaccc attccttttg gtgggaatac gccttttggt tccaccaaga cttcaccata
    12181 tgtatgattg aagctttgta cgatttcctg tgtctgctct agcattggtt tttgatcctc
    12241 accaacaggt acaagattcg ctttgaatgc agtgatatct gctgcttgag aaacaggata
    12301 gataaagaat cctgtaggca cgctttcacc gaattttttt tgttcgattt ctgttttgac
    12361 agtcggattg cgccgaacgc gtcctacgct gactaagttc aaataataca tggttaattc
    12421 agctaattcc ggaatttgtg attggataaa taaagtagat tttgcaggat ctaatcccac
    12481 ggctaaatag tctaaagcaa cttgcaagac atttgatgaa accttctcag gatttttagc
    12541 attatctgtc agtgcttgca tgtcagcaat catgacaaac agttggttgt cttcatctgc
    12601 ctgcatagcc actcgggttt ttagagaacc gacgtaatgc cctaaatgca gcttcccagt
    12661 tggtcgatct cccgttaaaa taatattttt caaaaaagac acctctttac gatttgtaag
    12721 tctgagcact taatatctta ccgtttttcc tgaaaagaaa caacagttcc taaggaaaaa
    12781 acaaggaaag aggataaaat aactaataga ttagaaatgg aagaaatatt tatgggagaa
    12841 taaacgttct aagaaacttt acaaattact aaactatcag atataatgta ttttattata
    12901 catctttaaa agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa
    12961 atttgatcac gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga
    13021 attcgagaaa ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat
    13081 gcttcgctgt atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac
    13141 tgacttgcat gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt
    13201 ccaacatttc aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat
    13261 caaagtgttg aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac
    13321 tgtcaatatg ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca
    13381 acgggtggca atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc
    13441 tacttctgct ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc
    13501 tcatgacggt atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc
    13561 ggatcgtgtt atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga
    13621 ctttttcgaa aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca
    13681 ttgataggag gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca
    13741 ttgcttttct tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag
    13801 agcgaagcaa atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg
    13861 gaatgatgga tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa
    13921 tcactaaaaa gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa
    13981 ctcggattcc attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca
    14041 cagatgaacg aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt
    14101 tgttggtaaa gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg
    14161 ttctagcagt caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa
    14221 aaattttaga attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag
    14281 acgcgatgac tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg
    14341 agttagttgg tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag
    14401 aaaacttctt gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg
    14461 ataaaatcta tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag
    14521 aggcaatgag cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca
    14581 cattgttttc gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa
    14641 tcttccaatt atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt
    14701 ttagaaacat tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt
    14761 ggtttaatct agatggattc caagcaggta cgattggatt aacgatctat acatctgctt
    14821 ttattgctga aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag
    14881 gactatcttc tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat
    14941 tcaaaatcgt tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta
    15001 ttttagcaat cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa
    15061 cattcaatac cttcgatacg tatatcatcg ttgggttatt ttacttagtt atcacgctac
    15121 cgttatctta cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg
    15181 ggaaatacat ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag
    15241 gattaaaggt tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg
    15301 gtctgacagg tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag
    15361 tgattgatat tatacggaat cttccattat tattgatcat tttcttcact tactttgcct
    15421 tacctcaaat cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt
    15481 ttgagtcagc tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac
    15541 agatggaagc aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt
    15601 taccgcaagc atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca
    15661 aagatacttc attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta
    15721 tttatggaca gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact
    15781 tcgtggtatg ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt
    15841 attaatagat gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt
    15901 tccattcgaa aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac
    15961 gctaaaaagt acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac
    16021 agataatcgc cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga
    16081 agccctgaaa caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc
    16141 atatggccct agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct
    16201 ggtggagcaa ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt
    16261 agaagaaatc aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag
    16321 agcggcagta aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt
    16381 aaaaagtcaa gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag
    16441 tcttgcttgt cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc
    16501 gaaaaaaatt gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat
    16561 tttgggatgc acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa
    16621 tgttcaactg atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta
    16681 tttcaatttg agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg
    16741 ctctgccaaa cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt
    16801 agaacatatc gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag
    16861 cttcgcaaac tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc
    16921 cgttttggtg atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt
    16981 ttgagagaaa caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg
    17041 agtacgagaa atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc
    17101 cagcgtctta ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt
    17161 agtatcatcg tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc
    17221 actggggcat ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg
    17281 tcagaagatc caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg
    17341 acttgcgtca cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt
    17401 gtaatgacag aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt
    17461 gatggtgaac agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc
    17521 aaagagcaaa aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa
    17581 acaaaaacaa tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg
    17641 ttcaaagaag cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg
    17701 gaagaaactg gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa
    17761 ttgcttgatc agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt
    17821 atgccaggaa tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac
    17881 gccaaattat tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat
    17941 tgtacgttag tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc
    18001 ggtcgagtag cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc
    18061 ccagaaggta agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt
    18121 catcgggctc aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag
    18181 agatagatgc ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac
    18241 ttagtagatc gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta
    18301 gaagcttctg acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga
    18361 ccaggattcg aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac
    18421 ggtcatttat cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca
    18481 actgccgata tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa
    18541 atcctgtttt tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca
    18601 tatgctctta tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat
    18661 caggcagttg aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa
    18721 tttttaataa aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag
    18781 tgttgaatga aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct
    18841 gctggaaaat caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca
    18901 tcatccattg tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt
    18961 attaggaaaa gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat
    19021 gattgacttg caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac
    19081 agaaacagat gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt
    19141 agtcgattcg ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac
    19201 aagaaaagag ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat
    19261 attgtctccg aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag
    19321 aaactaaaag ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct
    19381 tgaaaaagag gctgggacaa aagcgtttaa ctccaagaaa taagaaggaa ttcacgaaaa
    19441 ttgcttttca aatttttgtg aatt
//
LOCUS       DAYYNG010000043        19000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000043 DAYYNG010000000
VERSION     DAYYNG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.043"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..1407)
                     /gene="eno"
                     /locus_tag="ACHBFA_001215"
     CDS             complement(109..1407)
                     /gene="eno"
                     /locus_tag="ACHBFA_001215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM9394721.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVPADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1523..2278)
                     /gene="tpiA"
                     /locus_tag="ACHBFA_001216"
     CDS             complement(1523..2278)
                     /gene="tpiA"
                     /locus_tag="ACHBFA_001216"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9394722.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2382..3572)
                     /gene="pgk"
                     /locus_tag="ACHBFA_001217"
     CDS             complement(2382..3572)
                     /gene="pgk"
                     /locus_tag="ACHBFA_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9394723.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3682..4683)
                     /gene="gap"
                     /locus_tag="ACHBFA_001218"
     CDS             complement(3682..4683)
                     /gene="gap"
                     /locus_tag="ACHBFA_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9394724.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4724..5815)
                     /locus_tag="ACHBFA_001219"
     CDS             complement(4724..5815)
                     /locus_tag="ACHBFA_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM9394725.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5964..7298)
                     /gene="rpoN"
                     /locus_tag="ACHBFA_001220"
     CDS             complement(5964..7298)
                     /gene="rpoN"
                     /locus_tag="ACHBFA_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM9394726.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7358..7429
                     /locus_tag="ACHBFA_001221"
     tRNA            7358..7429
                     /locus_tag="ACHBFA_001221"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7391..7393,aa:Arg,seq:ccg)
     gene            complement(7621..8505)
                     /locus_tag="ACHBFA_001222"
     CDS             complement(7621..8505)
                     /locus_tag="ACHBFA_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9394727.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8592..9491)
                     /locus_tag="ACHBFA_001223"
     CDS             complement(8592..9491)
                     /locus_tag="ACHBFA_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM9394728.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9508..10014)
                     /locus_tag="ACHBFA_001224"
     CDS             complement(9508..10014)
                     /locus_tag="ACHBFA_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9394729.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10203..10823)
                     /gene="rpoE"
                     /locus_tag="ACHBFA_001225"
     CDS             complement(10203..10823)
                     /gene="rpoE"
                     /locus_tag="ACHBFA_001225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9394730.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10899..11336)
                     /locus_tag="ACHBFA_001226"
     CDS             complement(10899..11336)
                     /locus_tag="ACHBFA_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9394731.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11499..12329
                     /locus_tag="ACHBFA_001227"
     CDS             11499..12329
                     /locus_tag="ACHBFA_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM9394732.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12313..13680
                     /locus_tag="ACHBFA_001228"
     CDS             12313..13680
                     /locus_tag="ACHBFA_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9394733.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13699..14508
                     /gene="yidA"
                     /locus_tag="ACHBFA_001229"
     CDS             13699..14508
                     /gene="yidA"
                     /locus_tag="ACHBFA_001229"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9394734.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14551..14994)
                     /locus_tag="ACHBFA_001230"
     CDS             complement(14551..14994)
                     /locus_tag="ACHBFA_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9394735.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15128..15499
                     /locus_tag="ACHBFA_001231"
     CDS             15128..15499
                     /locus_tag="ACHBFA_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9394736.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15668..16450)
                     /locus_tag="ACHBFA_001232"
     CDS             complement(15668..16450)
                     /locus_tag="ACHBFA_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM9394737.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16447..17418)
                     /locus_tag="ACHBFA_001233"
     CDS             complement(16447..17418)
                     /locus_tag="ACHBFA_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9394738.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17510..18904
                     /locus_tag="ACHBFA_001234"
     CDS             17510..18904
                     /locus_tag="ACHBFA_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9394739.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         5368 a         3907 c         3382 g         6343 t
ORIGIN      
        1 actctctacc tgctgatatt tcagcaagta gagagttcga ttacttaact aagtgatcat
       61 actcgactat ctttcaatag tcgatcatgt atcttcatta gaaaagaatt atttgttttt
      121 taagttgtag aaagatttca aacctttgta ttctgcaact tcaccaagtt ggtcttcgat
      181 acgtagtaat tggttgtatt tagcaatacg gtcagtacgt gatagagaac cagttttgat
      241 ttggccagca tttgttgcaa cagcgatatc agagattgtt gaatcttctg tttcaccaga
      301 acggtgagat acaactgctg tgtagccagc ttctttagcc atttcgatag cttcgaaagt
      361 ttctgttaat gtaccgattt ggttcacttt gataaggatt gagttagcaa ttcctttttc
      421 gataccttca gataattttt gagtgtttgt tacgaacaag tcgtcaccaa ctaattgaac
      481 tttgtcgcct aatacgtcag ttaatttctt gaatccgtcc cagtcgtttt cgtctaatcc
      541 atcttcgatt gagatgattg gatatttaga aactaattct tcgtagaatt tgatcatttc
      601 gtcagttgtt ttttcgcctt cgcctgagtc agctggtacg taaacacctt tttctttgtc
      661 gtagaattct gaagaagcag catccatagc aagaacaacg tctttaccag gtacatagcc
      721 agctttttcg attgcttcga tgattacttc aaaaccttct tcgtttgaac caaggtttgg
      781 agcaaatcca ccttcgtcac caacagaagt tgctaatccg cgagctttca agattgaagc
      841 taatgcgtgg aatacttcag cacccatacg tagagcttct ttgaatgttg gagcgccaac
      901 aggcatgatc atgaattctt ggaagtcgat tgagttatca gcatgtgatc cgccgttgat
      961 gatgttcatc attggtgttg gcaatacttt tgtgttgaat ccgcctaggt agtggtataa
     1021 aggtacttct aggtagtcag cagctgcacg tgcaacagcg atagaaacac ctaaaatagc
     1081 gttcgcacct aatttacctt tgttaggagt tccgtctaaa gcgatcatag ctttgtcgat
     1141 agccatttgg tcacgaacat cgtagccaat gattgcttca gcaatgatgt tgtttacgtt
     1201 gtcgacagct ttagttacac ctttaccgcc ataacgagct ttgtctccgt cacgtaattc
     1261 aactgcttcg tattcaccag ttgaagcacc tgaaggaacc attccgcggc caaaagctcc
     1321 gctttctgtg tatacttcta cttcgattgt tgggttacca cgtgagtcta agatctcgcg
     1381 agcgtaaaca tcagtaataa ttgacatgtt ttgtctctcc tttgagtttg ttttttccta
     1441 agggatttcc ctcatatacg attttagtta attttgctca ttagtgcaaa catttcttgc
     1501 atttttcgca aattttcaac aattatttta cagcgtctaa caatgacaaa aatgaatctg
     1561 cttgcaagct tgcgccaccg actaaagcac cgtcaacatt ttcttttgcc atgtattcag
     1621 caatattttc tggttttaca gaaccgccgt attggatacg aactgcttct gatacttctt
     1681 ttccataaag tttttcaact gtttgacgta caactccaca aatttcgtca gcgatttgtg
     1741 cgtcagctga tttaccagta ccgattgccc agattggttc ataagcaata accatgtttg
     1801 acacttgttc agctgaaaga tcagctaaac cagctgtaat ttgtccttcg atccattcag
     1861 cagttttgcc tgcttcatag gtttctaaag attcgccgca gcataggatt ggtgtcatac
     1921 catttgcaaa gatagctttt gcttttttat tgatgtcttc gtctgtttca tggaaatatt
     1981 cacgacgttc tgaatgtccg atgatcacat aatgaacacc tagatcagca agagctgctg
     2041 gtgaagtttc accagtgaac gcacctgagt tttcccagta gcagttttgt gctgagattt
     2101 ttagttctgt accttcagct gatgtcatta attcttgtaa gaataatgca ggagatccga
     2161 tgactgaatc aactttgtca tttgaaggga ttttgttttt tactgcttca gcaaattctt
     2221 ttgcttctga tgcagttttg ttcattttcc agttacctgc gataataggt ttacgcattg
     2281 aggaacacac tccttaattt attgaaaaat ggctagggag tcatgaaaac tcccttcacc
     2341 ttttgatcga ttactcgtca gaattgagca aacttggtcg tttatttatc gttgattgct
     2401 gctagtcctg gtaattcttt accttcaagc aattccaagc ttgcgccacc acctgttgag
     2461 atgtgtgtga atttgtcagc aaatcctaat tgttctgctg ctgcagctga gtctccgcca
     2521 ccgatgattg ttgttgcgcc ttctaagtta gcgattgctt cacaaacacc gattgtacct
     2581 ttagcaaagt tactcatttc aaatacaccc ataggtccgt tccaaacaac tgtttttgca
     2641 ccttgtaatg tatcagcaaa taattttact gatgctgggc cgatgtctaa agccatcaaa
     2701 ccatcaggaa tgtctccttc gattacttgt gtttcaacat catttgaaaa ttctggtgca
     2761 cagacattgt cgattggcaa gacaagtttg tcgcctgctt tttcgattaa ttcttttgct
     2821 agttccactt tatcagcttc tactagagag ttaccgatct tgattccttt tgcttcgtag
     2881 aatgtgtaag tcattccgcc accgatcaag attttatctg ctttaggaat caagttttcg
     2941 attacgccga ttttatcaga aacttttgcg ccaccaagga ttgcaatcat tggtcgtttt
     3001 ggttcttcta ctgcttcacc gatgaacttg atttcttttt ccatcaagaa tccagcaact
     3061 gttgggatac cagttgaagc gatacctacg ttagaagcat gtgcacggtg agctgtaccg
     3121 aaagcatcgt taacaaatac atcgcctaat gaagcccagt atttacctag ttctgtatca
     3181 tttttgcttt cttttttacc gtcaatatct tcaaatcgtg tattttcgaa tacgacaacg
     3241 tcgccgtcat tcatgttgcg gattgcttct tctaattgtt ctccgcgagt ttcaggtacg
     3301 aatgttactt ctttacctaa taattcgcct agacgttttg caactggagc taaagatttt
     3361 ccttctttat cttcttctgt ttttacacgt ccaaggtgag agaaaaggat tgcttttccg
     3421 ccttgttcta gaacgtagtt gattgttggc aatgctgctt tgatacgagt gtcgtcagtg
     3481 atcacgccgt ctttcaaagg aacgttgaag tcaacacgga caagaacttt tttgtctttt
     3541 aaatcgatat ctttcactgt tttcttagcc attttatttc ctcctgaata aacttattat
     3601 caaaaaaagc ggaagaagcg cgattgcttc cccgctttta aatgtacatg tctataattt
     3661 acacttgtac gtttgtctat cttataagtt agcgaagtat tctaaagtac gtactaattg
     3721 tgcagtgtat gacatttcgt tgtcgtacca agcaacagtt ttaactaatt gtttgtcgcc
     3781 aactgtcatt actttagttt gtgtagcatc aaataatgaa ccgtaagtca taccaacgat
     3841 atctgaagaa acgatttcgt ctgtgttgta tccgtaagat tcgtttgaag cttctttcat
     3901 tgctgcattg atttcgtcaa cagttacttc tttttcaaga actgttacta attctgttaa
     3961 tgaacctgtt ggtacaggaa cacgttgagc agctccgtct aatttaccgt tcaattcagg
     4021 aattactaaa ccgatagctt tagcagcacc tgttgagtta ggaacgatgt ttgcagcagc
     4081 agcgcgtgca cggcggaagt cacctttagg atgaggtccg tctagagtca tttggtcacc
     4141 tgtgtaagcg tggatagttg tcattaatcc ttcaacaaca ccaaatttgt cgttcaatgt
     4201 tttagccata ggagctaaac agttagtagt acatgaagca cctgagataa ctgtttcttt
     4261 accagttaat gtttcgtggt ttgtgttata aacgattgtt ggaacatcgt ttccgccagg
     4321 agcagagata acaacgcgtt tagcaccagc tgttaaatgt ttttcagctg ctgatttaga
     4381 agtgaagaaa ccagtacatt ctagaacgat atctacgcct agttcgcccc atggtaattc
     4441 ttctgggttg cggttagcta atactttaac ttcttttccg tttacgttga atgaaccttc
     4501 atgaacttca actgttccgt tgaaacgtcc ttgagttgtg tcgtatttta acaagtgtgc
     4561 caacatttta gcatctgtca agtcgttgat tgctactact tcgattccat ctacatcttg
     4621 gatacgacgg aatgctagac gtccaatacg tccaaaacca ttaataccta ctttaactgt
     4681 catttaagat ttcctcctta tgaaaatcaa aaaaatattt attttaaagg gttacccctt
     4741 ttaaaatctc atttgcagca gcttcatcag tgatgagcca cgtttgtttt ggtgcatttt
     4801 tcatataagc tcgaattgct ttagcttttg attttccgcc cgcgattgcc atgatgatcg
     4861 ggactttttc caaatcttgc aattctatgc cgattctagg gaccttataa acgacttcac
     4921 ctttttcatt gaaaaagtag ccaaaagatt ccgcaacagc atttgcttgt ttcagcatga
     4981 ctaattcttt ttcagtcatc ttacgacgtg ctgccatgtg taatgcacgc ccaatactat
     5041 gaataacaca attcgcttca ctaatcaagt ttaagacttc ttggatagat ggctcattga
     5101 gtaaagaatt gtaagttgcc agacttaatt gttccggaac ataaagtgca cggtggttac
     5161 cgtttgcttt agcagccatt cttgcactga cagaatttgc ctgtacagtt atcgcttcac
     5221 caatcccgcc tcttgcaggg acaaacaggt tatgccgttt cttattttcc agattagaca
     5281 attgttcggc taccacagcc atagtagttc ctcccatcac agcgatgata ttctcgcctt
     5341 ctggaagtag tcggtctagg gcttcgctca gcgcttcgcc gaatgcatca gccactttgg
     5401 tctgctcttc gctgtttcct gaaacaacga tacagcgttg gttaccaaaa tattctgcca
     5461 actgttgttc ggtttgatgc gtacctaaaa gctgatccat gaagttttca aggctttgat
     5521 aaacttcttc gcccttagtt gtcagcgtca taccgctttt tgaggtgtcg atcaaattca
     5581 gatttttcaa aagatctgtt tctgttcgca atactcgttc tgtgatcccc atactttccg
     5641 ataaacttcg acgtccaatc ggctgcatcc aatagatatt tcgtaaaatt ttatagcgtt
     5701 cttgcaacac ttcgataata tctggtgcaa cagattcaat catttttatt tcatccagca
     5761 tagcgatctc cttagttgga cactttacgt ccaatgtaga cttaaaacga cccatatacg
     5821 ataaaaaaag aaattaacta ttcgaatttc acatcaataa agggaaatct caacctcacg
     5881 actgtaagtc taacagtttt gctctcgttt ttcaactaaa aagactagaa aaagtgaaat
     5941 actttttcta gtcttttgac tgattagtta tcataacgtt ttctttttga tgaactcggg
     6001 attcctaata aatcacgata ttttgctact gttcgtcttg agatcgctat ctcatctttt
     6061 ttcagcaatt caaccaattt ctggtcagac aacggcttgt ttttgtcttc ttgatcgact
     6121 aattcctgca gctttttctt cgctgtatct gctgaaaggt cttctgtctg ttctgtacta
     6181 ttcgtaggaa ttcgtgtagt gaaaaatttt tttaattcaa atacaccgaa gtctgtttcc
     6241 aaatatttcc cgttgactgc acgactgacc gtggattcat gcacatctat ctcagttgca
     6301 acttccttta acgtaagagg cttgattgga cgttccttgt cgataaagaa agctttttgc
     6361 cgggaaacaa tgacttgagc aacccgcaaa attgtatccc cacgttgtag gatcgttttc
     6421 ttcaaccatt cgaattcctg tttccgttct tttaaataat tttcagtttc tttatctgct
     6481 tgttgtgaca tttgtttgaa gtaactctct tgaaagcgta tatttggctg attttttcgg
     6541 tttgagataa cttgtacatt tttatcgtcg tccactaata cccggacatc aggaataatg
     6601 tacaattcgc tgctacggtc gaaaatccgg ccagggcttg gactcaacgt ttgtaaatag
     6661 tcgaatatct gctgaacagc atgtagatca acagaaaaac gttgtgcgat ttctttccat
     6721 tttcgctcca ccagttcctc aaaacattct tccaacacga tataagctaa ttccggcgca
     6781 taatcatctc gttctgtttg aagcatgaga cattcttgca aactacgtgc acctactcca
     6841 gctggttcta attgctgaat cagagtcaac gcatcgagca tttggatagg cgtagctcca
     6901 atctcttttc cggcttcttc taaactaatc gtcaaaaatc cgttcaaatc gatgtattct
     6961 acaagataaa gaacgatttt cctcaaaaaa gtatctcggt aattcaaatg gacttgttcg
     7021 atcaatgaat caaataaaga cccttttgta tcaggaatct gatttaggta atttgtctct
     7081 tctccttcat aagaagatga agaagctttt atgtaatctg gctgccattc cggctcaaca
     7141 acttccacca atggattttc cattgcttga ttctcaacga atgccaacag ctcctcactg
     7201 ttatattgta aaacttggat ggactgttgc aactgttgcg tcatggctag tttctgtgtc
     7261 tgctgctgtt tttgtgaaaa attttgctca aatttcattt ttttgtccaa tccccttgta
     7321 attttttatc tgtttagtta caattatccc agtgcttgcg tccttagtgt agtggatatc
     7381 acgtaagatt ccggttcttg agacgggggt tcgattccct caggacgcgt tctactgcta
     7441 ttttagcata atttacgaat agttgcttct ttatttttaa gtgaaaactg tccgccaaag
     7501 aaaattggtg gacagttttt tgttgtacct gtatagatat tgttaagcct ttgcaagtaa
     7561 ggtagttatg tgttcaacta cttattaaaa tgtgttattt attaaagata cttgtctcac
     7621 ttactgaact ggccgatgat cgtttctgcc ataatagttc tcgacagccg ttggatgccc
     7681 taataaatcc ccaactgtcg tgatgcgata cccttgctct ttcagataat cgatcacttg
     7741 aggaacggca cgaatcgttt caggatagat atcatggaat aaaataattg ccccatcgta
     7801 tactgttgct tggatcttct gcaaaatcaa atcaggattt cttgtcttcc aatcttctga
     7861 atcaacagac cattcgatga tcggtcgctg gatgatcgtt gctcctacat tcgtgacaga
     7921 accatagggc ggacgaacaa aagtgggggt tttgccagtg acttttttga tctcagtgtc
     7981 tgctttcgtc acttcctccg tcatcgactg ggcagaaaga gtggccagat tctgatgatc
     8041 gtatgtgtga ttgccaatct cgtgccctgc ttcagctgtc tgcttcacga tttcaggatg
     8101 ctgcgccgta ttttctccta agacgaaaaa agtggcatgc acgttttttt cttttaagat
     8161 tcgcagaagc tgcggtgttg tggtcgctcc tggtccatca tcaaagctga ttgcgacgct
     8221 tttgacagcc ggatctcggc tcgtttttgt actgtcttgt gttgattgtg atgactgact
     8281 ttgtaccttt tccgctgatt cttgaatggc ttctttgacg ttagctgttt cagttgattg
     8341 gcttttaatg gttgattgtt ctgattgttt gaaagattgt gacgaatgct ccgaagagtg
     8401 tgtttgtcct tttacttcgg tgatcaagga ggccaccgaa gtaatttcga aaaacaaaag
     8461 cacggataaa gcaataatac tgataactaa ataatttctt ttcactatat ctcttctttc
     8521 tctttttctc ttcttattat ttacttgtac ttacttgaaa attgaaactg ctagtcgcaa
     8581 ctacttctcg tctaatgatc caaataatct tccgatgcca aaatcgtttt tgtcacggac
     8641 ttaggaatga caaattctgg tgtacccata taaccaggag caatttcgta ttgagaaaaa
     8701 gtcaaaacta attgatgctc tttgttcaaa taaaaacttc gtgtaggcgt aacaagtgtt
     8761 ggtttttcta tcgtcccttc ttgaatatct tgctcagtcc aatagtactt agatggatct
     8821 tccttgactt gttcggccat ctgccgtttg acttcttttg tcaaaacaga tagataggtg
     8881 tcatctttga ataaaagtgg aagtgagagc tggacactgt ttttcttgtc gattgtgtcg
     8941 tatctttttt caacatggct atctgctgct gtttgggtga tctgcctttc aatcacgaga
     9001 aaccggttat cgtctacgat tttctgataa tctcccaaaa cttggtaatg atccgtttca
     9061 aaatttccat attctttttc agctttttca aaatctgctt gtccttcgcg aaagtatttt
     9121 tgatttaatc catgaattgt tttattttct ttctcatttt gtgtattgat cttcggtacc
     9181 tgaatatcga ttttttcaga tcgatcagta aagcgttcgc ctaagatcaa ttcaatgact
     9241 gatcctaaga tgggtaaatc ttctgcaaag cttcgaactt gactattaaa aagaaggcta
     9301 cttgttgtaa tcattacgat agccaaagat actccgaacc cttggagacg tctctttctc
     9361 cgctttgctt ttataaatga ttgctcttcg ataataaaac gctggcggat ctcctcttta
     9421 agccctttgg gaattgcttg ttctttataa gttttcttta gtttttttaa attttctttt
     9481 tgggaataca tggcactctt ctctccttca ttctaggatt tgacgtaaag cattcaatga
     9541 gcggtacatt ttcgttttaa ctgtgttttc attcatatcc aagatagtcg caatttcttt
     9601 caaacgaaat ccttcaaaaa agtagaggat cagaatctct ttttccggac tgtctatttt
     9661 atctaaaatc atatggagct ctgcaacgga agtttctaaa ggtaactgtt gggggatatc
     9721 ttgaatttca aataagtcca caggcgtcct tttttgtctg cgccaactat ctaatgccgt
     9781 gcggatcaat atccgataaa accatgcact aaaatgctcg atctcttttt ctttttcaaa
     9841 atagttcagg gctttatgaa aactatcttg catcacatct aaggcctctt ctttatcatg
     9901 tacgtaattc atcgtgatac ggaacatttt ttgataatca gtttcgatga gtccattcag
     9961 caggtgacgg tatttttcga tctgccgttt tttcttatat ccagtaaaaa tcattctcca
    10021 ctcctttctg cctcacttaa tatgacgtac aaggtgcgct aaaagtttca atttttttat
    10081 tttttatgta tttacttggg atttttatat acaaaaaaga aggtgtgaca aaaatcgtgt
    10141 cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta agaaaataat
    10201 gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc atcatcgtct
    10261 tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc atcatctgtc
    10321 gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc ttcatcttca
    10381 tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt atcttctggg
    10441 tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt gactttcttg
    10501 cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt tacttcttca
    10561 tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc taatgagata
    10621 aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg aatttcactg
    10681 tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc catgacatcg
    10741 ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct tttgtttgca
    10801 ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact ccttatcata
    10861 caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa atccaaggtc
    10921 aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat tcgaaccttc
    10981 cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt agcaatctgg
    11041 atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc aaatttcaaa
    11101 cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac taatttgatc
    11161 gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat gtacagcgta
    11221 tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac ttccccttgc
    11281 tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa ttccattatt
    11341 cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt gtcaaccaac
    11401 ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta gagtataata
    11461 ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg attcgactag
    11521 atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat atattaacgg
    11581 aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac tgcacagtca
    11641 tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa acactggaag
    11701 atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta tcagatgaag
    11761 gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata accatagatg
    11821 acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca ccgatacaag
    11881 cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc catggcaaaa
    11941 aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg atgtacttaa
    12001 gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat caatctgctt
    12061 taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca atggcaacac
    12121 ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca tttgcaaaag
    12181 cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg ctaaaaaaag
    12241 aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt aataacataa
    12301 aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga aaaagttttt
    12361 cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga tttgatcaat
    12421 tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc tttcactttt
    12481 cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat ttcaagacga
    12541 atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg aggctgggat
    12601 gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg tcatggtcct
    12661 tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac tgtagagatc
    12721 atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga aggttttcct
    12781 gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt ccaaatgatt
    12841 tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta tttcactgga
    12901 acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc ttatgaaggc
    12961 gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt tagccgatat
    13021 caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt aattttggag
    13081 catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt tgaactccat
    13141 tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata cctcaaactt
    13201 gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga tcctatttta
    13261 aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac tttttctggc
    13321 aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt cggatataat
    13381 cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt ttatcgccca
    13441 gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac tttgatagaa
    13501 ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg agatgagcga
    13561 tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga tttatttgac
    13621 gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga aataaattaa
    13681 aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga tggtacttta
    13741 ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc tgctgttgca
    13801 aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca ggaacagtta
    13861 aacgagttga acctttttca agataatgat tacgttatta cttataacgg ggcactcgta
    13921 caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga agatttccta
    13981 gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga tgacaaagcg
    14041 atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag tttagtcaag
    14101 atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat cgtcaaaatg
    14161 atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc gcaaactttc
    14221 cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact aaataaagaa
    14281 gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga tcaagatgaa
    14341 atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc tggtctagga
    14401 gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat cacttcatca
    14461 aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata aataaaaaaa
    14521 ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga tccatctctc
    14581 gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag ggctcaccca
    14641 ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat cagcaacaaa
    14701 gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat actcatcagc
    14761 ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca gctcacggac
    14821 aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa aaaaagcacc
    14881 atttggagcc tcgattactg caatctgctg atctctttct atgacaatat atgctgcata
    14941 acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat ccatgaatag
    15001 tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct catgaacaag
    15061 cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga aaaggagaga
    15121 tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag catcaatcga
    15181 attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc cagaaaacaa
    15241 atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt tgacttacaa
    15301 ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta tagcagctga
    15361 tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta ctgacttaaa
    15421 aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg gttataaaat
    15481 cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat aaaaaacaaa
    15541 caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt gcgtcttttt
    15601 ttaacaacca ttgtagctat aaggttgcca ctctccacca atttattttt aattcatttg
    15661 aaagaaatca aataaacgag gtacctttag aaaacaaaac tgcttttcct cctattatca
    15721 cacgatcttc ttttaattca cattctagat ccccgcccct tgatgacatt tgataagctg
    15781 acaattttgt tttattcatt ctttctgccc aataaggaat aagattagaa tgtgctgatc
    15841 cacatactgg atcttcattg attgttaatt ttggaaagaa ggttctagaa acaaagtctt
    15901 tttgcacact ttctgctgtc acgatgactc ctattcctaa ttcaaaattt ttgatagctg
    15961 taaaatctgg ctgcaattta gccaccgttt tttcatcttc taatacgaaa aataaatcac
    16021 gtgctaagta ggcttcttta atcttagccc caatcgcttc ttcatattca gacaatatcg
    16081 gcactttttt cggcataata ctaggaaaat ccatgtaata ataatcttta attcttgtca
    16141 caaatagatt accgctttga gtagaaaatt taatcgtttc atcaggtatt ttgtaatagt
    16201 taaataaaac aaatgctgtt gctaatgtcg cgtgaccaca aagatcgatt tctcgatctg
    16261 gcgtaaacca tctaagctca aattcttggt ttttcttcac tgtaaatgct gtttcagata
    16321 aattgttttc aatggcaatt ttttgcatcg taccttctgg cagccattct tctagcacat
    16381 aaaccgctgc tggatttcct ttgaacacct catctgcaaa tgcatcaact acgtaataat
    16441 ctaagttcat tttaaagccc ctttctctaa tttgttataa atatattctg cacaacaaag
    16501 atcttcaata gctagaccaa ctgcgtcgaa aatcgtcggc ctcgattctc ctttataaaa
    16561 tgtttttttc tcagaaacta attcgcctaa tgtaccaaca atacaagatt cctctaaatc
    16621 tccttctgcc atggctataa gcaggtcccc gctttctgtt aaggcagctg agtaatcatc
    16681 tacatagatt tgagaagttt ccatcaacga tgtacttatc tctcttgtat caggagtaaa
    16741 tgtgcccaca gcattgatat gtgcgtcttc ccttacccac tctttttgaa gaaatgcctc
    16801 tttactagag gtcaacgtac aaatgatttc cgctggacgt actgcatctt ctactgatat
    16861 tgcatttata aacgttattt taggatactg ttttttagct ttagcgataa aagtatttcg
    16921 tctttcttct aaaatatcgt aaacaaacac tgtagtgatg ggacgaatgg tagaaatcgc
    16981 agtcaaatgc gacaatgctt gttgaccagc tccaattaaa gcaagtgttt ctgcatgcgg
    17041 atttgctaag taatctgttg ccagcgcaga tacagcagct gttcgaatcc aggtgactgc
    17101 atttgcgttt atcaatgctt tgggtagtcc gttttgagca tcaaacaaga aaatctctcc
    17161 taaatgagaa ggtaaatgat actgatgatt atccggaaaa gcggtaatga cttttgtgcc
    17221 aaaatatctg ttctccccca aataagcagg catcatcgca aacatatttt tgccttttcc
    17281 gtcaggtaaa atttccacca atcgaggatt ttggctgatt ttaccacttt cataatcaaa
    17341 aaaacatttt ttcattactt gaatcgcttc ttcaaaacta aaagaagcta ctacttcctc
    17401 aaaattaata attttcatag acaattccct cctcacaaaa gattaacttc taaaatcaat
    17461 acaatcaatg gtaaaattgt atggtataca attttaagga gtggagaaaa tgcctattaa
    17521 ttcttatgac aattacccgt taacttggcg accagacaaa caaaaattga agcgtcctta
    17581 ttatcgatcg ttgattcaac aattagaaca agatatttta aagaataaat tacagaaaaa
    17641 cactcagtta ccctcccaaa gagagctagc tgattattta gatttaaatt ttactaccgt
    17701 tggcaaggct tataaatatg gaattgagaa aggtttgctg tatacgaata ttggaagtgg
    17761 cacatttatt tctcccaatg tctttgagtc catcaccatc tctaccaatg aagtaaccga
    17821 acatatcatt gattttggtc tggttagttc ttttgaagaa tgtaataaat tcgttgtccc
    17881 gtttattcaa tcaactagta ataacccaaa tcttgttggt ttgcttaatt accaagatcc
    17941 caaaggcaca aattttcaat tgactactgc agcaaattgg ttaaaaactc aaggagtcat
    18001 tccgtcctta gaatctctat ctattgtttc cggtgttcaa aatggattgg ctatcacact
    18061 ttctgcactt ttctctccag gtgatagaat agcaacagac cgatatacat acgctaattt
    18121 cattgaattg gctcagttat atcatttaga gatcgttcca attgattttg acgagcaagg
    18181 aatgcgacct gatatattga ttcaggaatg tagaaaaaag aagattcatg gtgtctttct
    18241 tatgccttct tgtaataatc ctatagggtt tcaaatctct cacaaaagac gagagcaatt
    18301 agccgaagtg attgaaaaag aaggtctatg ggttattgaa gatgatatcc attcattttt
    18361 aacaacttac tatcaacata cgatcattcc tcctttccaa aatttattac ctaaccaaac
    18421 aatctactta gctggaatga caaaatttat ttgttctgga ttgcgagttg cttatcttgt
    18481 agttccagta catgtccaaa gcaagataga acaagcaatt tttaatataa atgtgaaaac
    18541 ttctggatta gatgtggaag tcatcactca aatcctccat tcaaatgaag caaataggat
    18601 cttagaaact aaattaagtc ttacaaaaag agccaatcag ctttttgatt ctcttttcaa
    18661 agtagaaaaa ccaacgaatc gtgtccctta ctatcgaatt cttccagttg atcctgagat
    18721 tgaccctaag ataattgaga atgactttct tgctaacggt atccgacttt atcattccag
    18781 acgctttact acacgtgcgc aaccagatgc attcgttcga atttccctat cctctaataa
    18841 ccttgaaatt ttagcttctg gactaagtat cgtaaaagaa aaaataaaaa aatatgaatt
    18901 ataacctatt gataaataaa ggaatcaaat cttattgatt cctttcaata aaaaagaggc
    18961 actacccgag gttcaacaac gggtagtgcc tcaaaaaata
//
LOCUS       DAYYNG010000044        18864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000044 DAYYNG010000000
VERSION     DAYYNG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.044"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1119)
                     /gene="recA"
                     /locus_tag="ACHBFA_001235"
     CDS             complement(70..1119)
                     /gene="recA"
                     /locus_tag="ACHBFA_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9394740.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1228..2469)
                     /locus_tag="ACHBFA_001236"
     CDS             complement(1228..2469)
                     /locus_tag="ACHBFA_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9394741.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2709..3287)
                     /gene="pgsA"
                     /locus_tag="ACHBFA_001237"
     CDS             complement(2709..3287)
                     /gene="pgsA"
                     /locus_tag="ACHBFA_001237"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9394742.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3496..4419)
                     /locus_tag="ACHBFA_001238"
     CDS             complement(3496..4419)
                     /locus_tag="ACHBFA_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM9394743.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5158..6444)
                     /locus_tag="ACHBFA_001239"
     CDS             complement(5158..6444)
                     /locus_tag="ACHBFA_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9394744.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6437..7705)
                     /locus_tag="ACHBFA_001240"
     CDS             complement(6437..7705)
                     /locus_tag="ACHBFA_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9394745.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7957..8418
                     /gene="mscL"
                     /locus_tag="ACHBFA_001241"
     CDS             7957..8418
                     /gene="mscL"
                     /locus_tag="ACHBFA_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9394746.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8477..8923)
                     /locus_tag="ACHBFA_001242"
     CDS             complement(8477..8923)
                     /locus_tag="ACHBFA_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9394747.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9016..9261)
                     /locus_tag="ACHBFA_001243"
     CDS             complement(9016..9261)
                     /locus_tag="ACHBFA_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9394748.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9376..9954)
                     /locus_tag="ACHBFA_001244"
     CDS             complement(9376..9954)
                     /locus_tag="ACHBFA_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9394749.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10031..10561)
                     /locus_tag="ACHBFA_001245"
     CDS             complement(10031..10561)
                     /locus_tag="ACHBFA_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM9394750.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10565..11425)
                     /gene="rlmB"
                     /locus_tag="ACHBFA_001246"
     CDS             complement(10565..11425)
                     /gene="rlmB"
                     /locus_tag="ACHBFA_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9394751.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11418..11813)
                     /locus_tag="ACHBFA_001247"
     CDS             complement(11418..11813)
                     /locus_tag="ACHBFA_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9394752.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11810..13222)
                     /gene="cysS"
                     /locus_tag="ACHBFA_001248"
     CDS             complement(11810..13222)
                     /gene="cysS"
                     /locus_tag="ACHBFA_001248"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9394753.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13219..13755)
                     /gene="epsC"
                     /locus_tag="ACHBFA_001249"
     CDS             complement(13219..13755)
                     /gene="epsC"
                     /locus_tag="ACHBFA_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM9394754.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13841..14080)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14077..15537)
                     /gene="gltX"
                     /locus_tag="ACHBFA_001250"
     CDS             complement(14077..15537)
                     /gene="gltX"
                     /locus_tag="ACHBFA_001250"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9394755.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15707..16864)
                     /locus_tag="ACHBFA_001251"
     CDS             complement(15707..16864)
                     /locus_tag="ACHBFA_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM9394756.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16898..18268)
                     /gene="radA"
                     /locus_tag="ACHBFA_001252"
     CDS             complement(16898..18268)
                     /gene="radA"
                     /locus_tag="ACHBFA_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9394757.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18331..18858)
                     /locus_tag="ACHBFA_001253"
     CDS             complement(18331..18858)
                     /locus_tag="ACHBFA_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9394758.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         4985 a         4163 c         3389 g         6327 t
ORIGIN      
        1 caataaaaaa acggcaggtc aaggagagta cctactcttc agaccagccg tttttgttta
       61 tcataatttt tattcttcta ctaaaggcaa ttcttcttgt cctttttcat cttctgggac
      121 agcgacttct tcaccaatac cataagctgc tctaactaga gcagaaactt ctgccatcat
      181 ttcaggatga ttagccatat agattttagc gttttcccgt ccttgaccaa tccgatcttc
      241 tttataagaa taccaagcac cgcttttatc aacaatgtct ttttctactg ccatgtctag
      301 aagttctcct tcttgtgaga tccctaaacc atacatgaca tctacttcag cgattttgaa
      361 aggaggtgct actttgtttt ttacgacttt gatctttgta cggttaccaa caatatctgt
      421 accttgtttc aattgttctg cacgacgtac ttccaaacgg atcgttgcgt agaatttcaa
      481 tgcgcgtcct ccaggagtga tttctggatt cccaaacatc acgcctactt tttcacggat
      541 ttggttgatg aaaatagcga ttgtttttgt tttgttgatc gaaccagaga gtttacgcaa
      601 tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag tcacccattt cgccgtcgat
      661 ttctgctcgt ggaactaaag cagcaactga atcgactact acgatatcta cagccccact
      721 tgatactaaa gcatcagcga tctctagacc ttgttctcct gtgtcaggct gtgaaagaag
      781 tagttcatcg atattcacac ctaatttttg tgcatattgc ggatctaacg catgctcagc
      841 atcaatgaaa gcggccgttc cgccattttt ttgtacttct gcaatagcgt gtagtgcaac
      901 tgttgtttta cctgaacttt caggtccata tacttcgatg atacgtccac gggggtaacc
      961 gccgacacct aaagcgacat ctaacgcaag agagccactt ggaattgtag agatttgttg
     1021 gtcgattttt tcgcccaatt tcataataga gcctttacca tagctctttt caattttttt
     1081 cagtgcagca tctaaggctg ctttacgatc atctgccaat acgataatcc tccttatatt
     1141 tacaaatcag agcaacattt cattatcctt atcatatctt ttttagtgaa aaaaagcaag
     1201 aaaaaaccga acaaatattc gcatttatca ttttcttaaa agctgttttc tcaaaagatc
     1261 aaatccttgc ataaccgcat tatatctgat agcagcacga tctctggcaa agtgatattt
     1321 ttccgaaacc acgccgtctt tagatgaaat agcgatccat actgttcctg cagattgacc
     1381 ttctaacgtg tccggtccag ccactccggt gaaagctaca ctgtaatctg ttcttgctat
     1441 ctcacgtgct tgaatggcca tctgctccac acattctttg ctgactgttc catattctgc
     1501 taatagctcc ggatcaatct ccagaaaacc agcttttgtt tggagcgagt aggtaacgaa
     1561 tccgcctgga aatacttcag atacaccagc aatgtcgcct aatgcggctt ggaaggcgcc
     1621 ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt tctttcagta gttccactac
     1681 gacttttgct agcgaattat catcgccgta tccataaaaa tattccccta ctcgttcttg
     1741 gattttttct tctaatgcta aaagagcttg attccctgcc tgaacatcat ttgttttcac
     1801 tgtcagtcgt aatgtcactt catttggttt ggcatatggt gcgatcgttg gattgatctg
     1861 tgtctcaatc agatctttta gctcagtgac cagtcgcgat tcaccaatcc cataaaagcg
     1921 caaaacttta gaaattagtt tttcttcaga aggaaaacgt tcctctagta aaggacgtac
     1981 ttgttccaca aacattggtt tcaactcatt aggcggaccg ggaagaagga tatacgcatg
     2041 tgtatctgtt tgataaaaag tccctaaagc aagccctgta cgatttggta gaggaacccc
     2101 gccttcgata atctgggact gttgcagatt gtttttggtc atttttcgat gggttgtttc
     2161 aaaatatgcc aataattttt tgtatccttc cgtattttgt atgagagact taccgaggtg
     2221 agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt cctaatccgc cacataagag
     2281 tatgagttca cttcttgttt cagctagagc aatcaactct tctaaacgct gctgattatc
     2341 tcctacaact gtttgataat aaacttcgat acctagatca gctagctgtt ctgacaagaa
     2401 agtggcattc gtattcacga cttgtcctag taaaagttct gttccgacag ctattatttc
     2461 tgctttcatt acttcccgct cccttatata gtatacgctg tatttttata tttttttatt
     2521 taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca gctccttgtt ccaaataaac
     2581 agtgttctcc tgccaaaacc tcggtcataa gtcacaatat acataaaaca tgaaaagctg
     2641 ttttttgaaa ttatcccttg tgactcagag ttagacggca ggctcacaac ctctccacta
     2701 aacgatactt acattgatcc tttgaacaca ccgctgtttt tgatgaagta atccacacct
     2761 gaatagatag taaagatcag acaagcataa agcatgattt gatctactgg aataccgatc
     2821 aggctgaatg ggatattatt gataaacagc aagataatag caaccatttg tgtagccgtc
     2881 ttgacttttc ccggccaagc tgcagccatg acttctccat gttcgacaag tagtaaacgc
     2941 aagcctgtga cggctaattc acgacagaca atgatcgcta ctacccaacc aggggccttt
     3001 ccttggtcaa ccaacatgat aaaagcagtc ataactagca ttttatctgc caacgggtcc
     3061 gcaaacttgc caaaattagt gacaaggcct cgcgcacggg caatttttcc atctaaccag
     3121 tctgtgatac tagctaaagc aaaaataact gcacctacta gctgtgtgac agccagtgtg
     3181 gtgtctccta cgttaaattg tccccaatct aaaggtaaac ctacaaccaa aatgaaaatc
     3241 gggatcatac aaattctaag tacagttaat ttatttggta aattcacact atcgactcct
     3301 ttcttcccta ttctacgtga ctatcagcga agtttccacc gatattattt acttttccaa
     3361 atgaatttat tcaaaggagt atttttattg aaattgatct ttttgaatta acgaagaagg
     3421 accgcgacac agatttgtca cgatccttct tttttatacc tacaagtaaa cagatgcgta
     3481 aactaattta tacgatcatt ctgcgtattg aagagtaagg ttcacatttt ttggactctg
     3541 attgttttcc cctggattaa ctggtacgga ttctccattc gctttgatcg ttgcattgct
     3601 tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc ggtaacacgg tactctgtgt
     3661 ttcatttgct gccagtgtcc cttgataaac ataggcattg tctactagca cacctaacca
     3721 tacacgattg acagcagtaa attcaagggt tacaggcttg gtagcgtctg tgatatttac
     3781 tgcgatggca ctgccagaat cttctcctgt agctattgcc attttctttt cttcacttga
     3841 ttttgttgtt gattctactg tagaagactc tttggtctgc gtgctttgct ctgaggaaga
     3901 ggactcctcg actgatcctt ctactttaag cgaagaagct gtgtctccga ttattggatc
     3961 agcttgacga tcttgccaag tcatatagcc cacgacaacg acaatcgcca aagcaaccaa
     4021 tcctaataaa atgacaggaa gacttgtcca aaagcgactt ggatgcgatt cttcctgatg
     4081 cattgctttt ctagaagtac ttactgtttc aggctgcgga cgttcaggga ccgatcctgt
     4141 caatttgctt ttcccatcaa aaacatcgac taaatggtct ccatcttctc ctacagcttc
     4201 agcgtattgc cgaataaatg cccgcacgta aaatttacca ggcaattggt caaaatcatc
     4261 attgtcgatt gcttccaaat aacgtttttg aatttttgta atctgctgta attcatctaa
     4321 agatattttt ttattcaggc gtgcttgccg taatttttct ccgatggttt caaaagccac
     4381 tcgttcatct tctccagtct gtttgattct ccgaagcatc tgctgttgca acgaaagacg
     4441 ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc ttttttccga ataaacattg
     4501 ttctcctgcc aactcctcgg tcacaagtca caatatccaa taaatataag aagctgtttt
     4561 ttgaacttgt tccttgtgac tcggagttaa acggcaggct cacgacctct ttcttaaacg
     4621 gtgttcaatc agctgttcct cggtattagc tcaaaaaccg gcaaaatata aggagatatt
     4681 tttccgtatg tttctctaat actcaggtca aaacgctgat ttcacaacct cttccttaaa
     4741 cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cattaagaag
     4801 ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttgg
     4861 tacacggtgt tccagaagta gcttcttcgg caataagccg aaatatcaca aaaatttgag
     4921 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
     4981 ttgattactt cgtttgattt tattttaata taagttgatt aaaagagagc atgatttgtt
     5041 ttattttctc actaattttt ccaacacaac taagaatacc ataaaagctg attttggagg
     5101 aggttcctac tacaagtcta aaacgaaatt taagactttt cctcataaaa tactggttta
     5161 ttttggtctc atgtaaaact cgctgaaggc ctcttcactt ataaatgctt ccccagctgc
     5221 cagtacgtct ttcatttgga tcgaatcgat gatttcagga agatcaaata aagttcgatc
     5281 gccaaataaa ctttgcgtaa attgattggc aatatattcg atcgaattta atgattggaa
     5341 atattgtcca agcattttct tcttcagcaa atcgagattt gtttctgaca cttccggatc
     5401 atcagcgaat ccaagaatga tttctttgac tttttctgct gctttctctg gttcgtccgt
     5461 atctccgctg aagtccgcaa agtgaaattc acgatccaag ctgaattcaa aaccaaagct
     5521 gtcatcgata attccttgat tgtacatcgc tagatagttt cgagaagtat tgcctaaaat
     5581 catttggaac aacagattga ttgctgtttt atagcgtaac agttctgttc cttcttgcgg
     5641 caatgtgtct aatcctttaa taccaagtac aaatttatct cgtgtaatgg ctgcttccaa
     5701 tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa taacgtacaa tttcttgctt
     5761 tggagggaaa ttttttgctt cttggttttc ccgaataagt ttcatcaatt tctcaggctc
     5821 catcttacca actacaaata aaaccatatt gcttggctga taaaacgtcc gatagcacgt
     5881 atacaagtct tgagctgtga ttttgtcgat actttcaacc gtgcccgcaa tatcgatatg
     5941 caatgggtgt gtaggataca agttatttag tatgccaaaa aacatccgcc agttagggtc
     6001 gtcttcatac atttggattt cctgaccgat gattcctttt tccttgttta cagtttcttc
     6061 tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc agattctttt ctacctgatc
     6121 cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg aaagcattgg cggaagctcc
     6181 ttgtttccca aataattgaa agacatcacc atcttctttt tcaaacaact tatgttctaa
     6241 aaaatgggca attccatctg gaactttcac cttttctttt tcaccatagg gaataaattc
     6301 gttatcgatc gaaccataat tcgtactgaa caaaccatat gtcttgtgat agtcattttt
     6361 aggcaacaaa tacacggtca aaccgtttgg cagtacttca tgataaagtg tttcattgat
     6421 ttgctcatat tctgttttat tcatttgcat ttcctcctgc taaaaagaag attgcctgca
     6481 attcgatttg ctcagctact tgttgtatct ctttgatcgt tacgctctcc atacgtttca
     6541 accattcttc atcagataat tttgtttccg gcagccagct gtccaaaaaa gaagcttcga
     6601 ttgccgcttg cggagagtct agagaaagca gatattggtt tctcagcatt gcttttgtct
     6661 gagacagttc caactctgtt atttcaccat ttcgcaaact ttctaattgc tcgtgaatca
     6721 gatgaagtac ttgatttcgg ttcttttcat cgataccggt ttggacactc atgaatcctc
     6781 ggaacgtatc cacgctacta gaggcataat aagctagact ttctttctct ctgacattca
     6841 tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt aaagaccatt agcgcaaaac
     6901 gttctggttc atcgtaatac acatttgtct gataagctag gttcagcttt gcttgaacga
     6961 ttggctcacg tacttgtcgt tccttcacga tattcacttg aggttgggta taaaacagat
     7021 ctggataaac tgtttcccgc gactcaaatg gtaattgccc aactgcttca gctgctttgt
     7081 ccggatcaac atcgccgaca acaaaaatgt cgatttgatc atttgccatc atttcttgat
     7141 agtaggacgc tagcgaacta gcagtgatct tgtccaaatc ttcgactacg cccaaccctg
     7201 ggatcttttg ttccgggctg ttttcaaaat aaagttcttg caaggcatag gaagcaaaag
     7261 tttgtttatc ttccttcaaa ctttccaaat atagacgcag attgttcttt tccaagtcaa
     7321 acgtcaactg atcaaactga tgatttttaa tatttggata aaataaaacc tcttttaaaa
     7381 aatctaccgc ttgagaaaaa aggtttggat cattgacata ttcaccattc acaaaactga
     7441 tccctgcgtt tagccaatgt aaatttcctt tttttctaac tgataatcca aagcttgcgc
     7501 cataaagctc tgcaagttgc gcacttaact ttgtctgatc cggataattc aaactatttg
     7561 tttccaacat gctgctgaga agtgaacgtt tcgtgatgac ttcttgttgc agacgcgcag
     7621 taaaacggat aaaaatccgg atcgttttgt attgttttgt tggtaagaca tgaagattca
     7681 cgccttctgc caattgatac accatattta tatgctcctt ttcacagttg attgcccgct
     7741 tactctttta atgggtcaac ctgccttttt tacttattcc tctgcttgaa atttataagt
     7801 tcaggcaatt cgccgttttt tctaaatgat gttacacttt atagtatagc atagatgttt
     7861 tttatcaaaa gaaacagacg gctaaaagcg tatattgttt ttttattttt tatttttcga
     7921 tatactaaag acaaataaaa gaagggaggg tcattcatga tcaaagaatt caaagagttc
     7981 atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg ttattggtag tgcgtttaca
     8041 agcatcgtaa accaagtggt gcaaggcttg atcacaccac taataggttg gtttgtcgca
     8101 atgatcacag gtacttctga tttagaagga gcactttcaa ttcttgactg gtcgccgaca
     8161 aaaggcgtca catttgcgtt tggtaacgtt attagtgcta tcattacatt tctgatcact
     8221 ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg cgaagaattt acgctccaaa
     8281 gaagagcaag tggaagtgga agaaactcct actgcagaag attatcttag tgacatacgc
     8341 gatcttcttg ccaaacaagt ggcagaagac aatacacatc cagtagagca aacaaatctt
     8401 tccgatactt ctaaataata aagaatctaa aaaaaagaaa gccgattttc agtgtcggct
     8461 ttcttttttt agattcttaa cttgctactt gttccttttt catgtcgata tgtggaatat
     8521 catcttccaa atacacatct gagattgcct tgaatccaaa cgactcataa aaagcttgta
     8581 ggtattcttg tgcgccgatc acgattggtt tttctggaaa atttttctgg atcatgttaa
     8641 tcgtttcttg aacaatcttt cttcctaaac catctttacg gttgtcctga tgaacaagga
     8701 cacgtccaaa atggatactc tccgcttctt catatactcg cgtgtaggca aggatcttcc
     8761 catcagtatc ttgaaaaaac gtatgccaag cttgtccatc aatgtcatca acttcttgat
     8821 aaggacactg ttgttccaca acaaaaactg cgattcgttc tttgacgatc gcgtaaaact
     8881 ctgctgttga caattcttca aatttcttta cctgatattc cactaagcaa acgccccctg
     8941 cttttttatt cttagcatac cgattcgcac acaaaaaagc aaacgaacct tacgaatctc
     9001 aggctcgttt gctttttaaa ctgcttcagt taaaaattcg atctcaactg tccgagtaag
     9061 tacatctgaa tagctgtaag atactcgttc aaaagaattt tcttcaggat ccaaatcaac
     9121 aacgaatact gaaggatagg tttctgttaa aacgccgcgg cgttctgttt gacgctttct
     9181 tcctgtctga gcaaccaaca tgattgggct gccgatacga ccttctaaat ctttcttgat
     9241 tgaagctaaa gttgttggca tacagttcac ctctactatc aactatacca catattttta
     9301 aaattttcaa gtttaccata aaaaaaaaag aaatgcaata aaaataaata agaagctcat
     9361 taagattttt ctaaattatt cctcattata aaatttcaag tgttttttta ttttgagttt
     9421 tactctcccg taagcgctca ttacttgctt gtgatccatt gctaaacatt cagccatgtc
     9481 tacaaaatca acgccttgca tataataatg aaaaatttta gcttctattt ttgaaaactg
     9541 gatttcgcaa ttttcgattg cttcttttaa taaaagatgc tgaatcgcta aaggttcttg
     9601 caatgtgatc tctgctaaaa agtctggtcc ttctgtctct attttttggt cgattgagac
     9661 ttcatctttt tgatcacgac gcttcagcgc gttctgcttc ctcaacagag aatagatttg
     9721 attgcttata atgcttttat aataacttcc aaagctaaca cctttgcttt tttcgtaagt
     9781 tctcaatgcc tgatataaag cgatccttgc ttcttgttcc caatcctcag aaacataatt
     9841 tcttaaataa taggtgtttt tcaaacgtag taaaagtgga aaatattttt gatacagggc
     9901 agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat aactcctcag acataaaata
     9961 attcctccat aaattcgttt atggaggaat cttatcatca atgattcatc gaactgatta
    10021 gaacgatatg ttatttattt tttctcatta aatcgtccaa tttttgggac aattttttta
    10081 actgctcttc attccatgga gaattacgtc ggaaatcaat aaaatgcata tctttatttt
    10141 cttgggctat tgttttttcc gtttttttta tcgtcttata taattcataa gcagaagttc
    10201 gtaatgctcc ttgggaaaag atcatccact gttctgctaa atcgcttgtc gccactgtca
    10261 cttgtgtcaa acggtcattc agctgaccag cgattctctc gatataactg tcagccgttt
    10321 cgtcttcttt cgtaaaaaca acatccaact ggtatttctt ataattttgt tggatgcccg
    10381 gaactagttg ggcatcgaag acaacgatga cttccattcc ttcatatttc gcataatttg
    10441 ataaacgatg taaaagagct tctcttgcat ccgccatttt gtcctgcttt ttaagctgga
    10501 caagttccgg ccacgaacca atcatgttgt agccgtctac aatcaacagc tgttttttca
    10561 tggtctactc cccctgacgt tgggaaaaag cctgatacat aagcaaacca gcagcaacac
    10621 tggcatttaa gctctggacg tgcccggtca tcgggatcgt cagcaattca tccatttctt
    10681 ttttcaatcc tggactcatt cctttgcctt cattaccaat gactaaagca attgatcctt
    10741 tggcattcca tttttggaag tttgtgccgg tcatatctgt gccaaatacc caaaatcctg
    10801 tttctttgag ctgttgcaca gcttgtacca aattcgtcac gcgtgcaaca gggacatgtt
    10861 cgactgcccc agtggaagtc tttgttacga taggtgtgat tccaactgct cggtgttttg
    10921 gtatgatcac accatcgacg ccacttgcgt ctgctgtccg taaaatcgac ccaaaattgt
    10981 gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc ttcttttttc tcttttgttc
    11041 gttcaatcag ttggtcaagg gataaatatt cataaggtgt aatcgcaaga acgactcctt
    11101 gatgcacgcc gtgatcgctc aaagtttcta atttttgttt aggaacccat ttgactggca
    11161 cagcttgttc acgtgcgatt tctttcagtt cttctacttt ttctcctcgt gaatcttctt
    11221 gaagaaagag tttattccct cgcccttgtt gtagcgcttc tactgcggca tggtgcccaa
    11281 agacgaaatt atcttctaat gcttcttcat tttgatcaga aactgactga tttcgtccag
    11341 tttgcttttt atttgaatat ctattttgtt ttttgtcttc aaatccacgt ttcgattgat
    11401 gtttgtttgt tctttcttta cccatttttt tcaccaactt tctgaataca ccagcggatc
    11461 aattcttcca tccgctcggt ttgttttgtc aaatgcagat agcccatgag cgcttcgaac
    11521 cctgttgcga cccgatacgt cgtaacatca gcattttttg cgctcgtatg gcttttagca
    11581 tttcttcctc tgcgataata aagttcttct tcttctgtca aaagatgttc ttccagcatt
    11641 tgctgaatca aaaaggcttg tgcattcgca gaaacataat tcgttgccgc acgatgcagc
    11701 ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac gaacatagat ttcgtaaatc
    11761 gcatctccaa cataagccaa ggctaaaccg ttcaattgtg tgtaatctct catgcttctc
    11821 ttctccatcg ggttccttgc ggtgtatctt ctaataagat ccctttttct ttcaacaaat
    11881 cacgaatttc gtcgcttcgg acaaaatcac gattgcttct tgcttgattt cgttcttcga
    11941 tcaattgttc aatctcttca tctaataact cttctgctgt aaataaaata ccgaagatcg
    12001 aaagccattg cgagaataaa gcttctcctt gtgccaagac agcggcagaa actttcggct
    12061 ggtctagata agtgttcagc cacttagcaa gctcatagac cactgtgatc ccatttgctg
    12121 cattaaaatc atcgtccatt tcttcgatga atcgctgttc taaattttct aacaaagcga
    12181 ttttatccgc atcatctgcc aaattctctg aggcagtttc ttggcggaat ttcaaattgt
    12241 caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc tttcatcgtc gtttcactgt
    12301 agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa acgcaagact tgtggatcaa
    12361 cgtttttgat catttcatgt acagtgatga aattccctag agatttactc attttttcat
    12421 cgtcttcgcc aatcgttaca tagccattgt gcatccagta attagcgaat tttttccctg
    12481 ttttcgcttc actttgtgcg atttcatttt catgatgagg aaattctaaa tcttgcccgc
    12541 caccatggat atcgatcgtt tctcctaaat gttttgttgc cataacggaa cattcaatat
    12601 gccaaccggg acgtcctttg ccccatggtg aatcccaaga aatctcgcca ggttttgctt
    12661 gtttccataa agcgaaatct aatgggtctt ctttttgctg ctgttcttcc cctgtacgct
    12721 gacttgctcc gacttccagt tcatcgatgg attggtcact taatttccca taattttcaa
    12781 atttacgggt acggtaatac acatcacctg aagattcata agcatagcct ttttcaacca
    12841 acacttgaat aaatgctaaa atatctggca tatgatccat gactcttgga tgcagtgtag
    12901 caggctcgac attcaacgct ttcgtatctt cttcaaatgc tcggatgaaa cgatcagcaa
    12961 cttctggtgc tgtaatacct aattcgtttg ctgtacggat gattttatcg tctacatccg
    13021 tgaaattcga tacgaaatcg actttatagc ctcgatattc aaaatagcgt cgaattgtat
    13081 cgaaagcaat ggtgctacga gcatttccga tatggatata attatacaca gtcggtccgc
    13141 agacatacat accgacatgt cccttttgta tagggatgaa ttcttctttt tttctggtca
    13201 atgtattgta gatttttatc attctgatct cctcactttt tctcctttga ttcgaacgac
    13261 ttttgctggt actcctaccg ctgtagcgtc gtctggaaca tctttcaaca caacggcacc
    13321 tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct aatatctgcg cattcgcaga
    13381 gagcatggct ccttgtttaa cagttgggtg tcttttaccc gtttcttttc ctgtgccgcc
    13441 taatgttacg ccatggaata aaatcacatc gtcctcgatt tctgcggttt cgccaatgac
    13501 gattcccatt ccatggtcga tgaacacgcc agttccaatc gttgctcctg gatggatctc
    13561 aacaccagtt aggaaacgcc agaattgagc attgatcttc gccaataaaa agagccggtg
    13621 ctgatataaa aaatgagaga aacgatgcca aaacaaggca tgaaaaccag ggtatgtcaa
    13681 aacgatctca gctgtcgaac gtgcagccgg atcgtttctc tttgctgcat caatcgctcg
    13741 ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat tttatgtagg aaatcatctt
    13801 cttataaaga aaagaagcgc cttttaaata agtttcctta tcaaaagacg ctttccgcgt
    13861 ggttccactt ttcttcataa tcaatgatta tctctgacag ctaacgcctg ttatacgttc
    13921 ttggctactt tgtttcctat aaaacagttt ttcctgtttt ctgtgtttcg ccaagaccac
    13981 tcccagaggc atttcggcaa ccattcattg ataccttgca ccacccggta tctctcttgt
    14041 aatgatcagc tgcttacttt tttctgttca tcgtgtttag ttcagtactt gatttaaatg
    14101 tgctaatgat ttttcacgac caagcaattc gatcgtttca ggcaattctg gaccatgcat
    14161 ttgtcctgat acagctacac ggattggcat gaaaagattt tttcctttta cgccagtttc
    14221 tttttgaaca gctttgatgg cagctttgac agttggagca tctactactt ccatttgttc
    14281 tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt tcaccagcaa gtacttcttt
    14341 tgctgcttca ttcaatacag gatgttcatt gaaaaatagc gtagacagat caacgatttc
    14401 tgcagcatag ctcatttgag gttgatacag gctaacgatc ttcttcaccc attcgattgt
    14461 cttttcatct gggttttctg ctacacgtcc atcagcgatt agataaggca gacacatgtc
    14521 agtaaatgca tctagatcca ttgctttgat gtaatggttg tttacccatt gcagtttttt
    14581 cgcatcgaaa gctgcgggtg atttacttag gcgttttgga tcgaacattt tgatcaattc
    14641 ttggtgttcg aagatttcgt cttcgcccac aggtgaccag cccaataatg caataaagtt
    14701 gaacattgca tctggtagat agcctaattc gcgatattgt tcgataaatt gcaagattga
    14761 ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg atgatcaatg tcatatgtcc
    14821 gaaggttggt ggtgtccagc caaatgcttc atagatcatc agttgttttg gtgtattggc
    14881 aatatggtca tctccacgta aaacgtgagt gattttcatc aaatgatcat cgaccgcaac
    14941 agcaaaatta taagttggca taccatcgcg tttttgtatg acaaagtcgc caccgatatt
    15001 atcggattcg aacgaaatag cgcctttcac catatctgta aatgcgtatt ctgtattgcg
    15061 aggcacacgg aaacgtacaa cagattctag accttgtgct tctttttctg cttgttcttc
    15121 aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt tctcctcttg cgcgttgtgc
    15181 ttcccgttct gcttctaatt cttcttctgt gcaatagcat ttgtatgctc gattgctagc
    15241 aagcagctga tcaatcagcg gttgatagat ttctttacgt tcagattgac ggtaagggcc
    15301 gtattctcca ggattttcag gagattcatc ccaattcatt cccaaccaag caaggttttc
    15361 caactggctt ttttcgccat cttcgatatt acgtttttgg tcagtatcct caatacgaat
    15421 aataaagtct ccatcattgt ggcgggcaaa caaataattg aataatgcag ttcttgcatt
    15481 tccaatatgc aggtgcccag ttggacttgg tgcatagcgt acgcgtactt tcgtcatttt
    15541 cttcctcttc tttcaaacta gactttgact aacagcaaaa attccgtcag ttacggcttc
    15601 tcaaatatca tttatttaaa gccactcagc acaaatttta ccttttctaa ggtaaatagt
    15661 aaagccaact ggttgaattt ccttaccagt tggctgaatt tcttttttat tttgattgtt
    15721 tagtcttttt ctctgtctct tttttattgt cgattcctcg tcctgcatgt gcaggtttag
    15781 caaagatcat gcgtccagca gctgtttgaa gggcacttgt tacaacgact tcgatacgtt
    15841 cattcatgta atgctgtcca tcttctacga caaccatcgt gccgtcatca aggtaggcta
    15901 ctccttgttg gcgttctgtc ccggctttta ctactagaac attcatcgtt tcaccaggga
    15961 tgacgactgg ttttacggca tttgccaacg cattgatatt aagaacaggt acattttgga
    16021 attcagagac tttattcaag ttatagtcat tggttacaat gacaccatct aaaagcttag
    16081 ccaatttgat caatttgctg tctacttcac tgatatcttc aaagtctcca tcgtacattt
    16141 cgacagatat accctcttct ttttgcaagg catttaggat atctaatccg cgacgaccac
    16201 gcacacgttt caaactatcc cctgagtccg cgatgtattg tagttcgtaa agaacaaaat
    16261 tagggatcat caaggttcct tctaagaatc cagtctttgt aatatcataa atccgaccgt
    16321 caatgatcac gcttgtatcc aagattttat atttatggaa attttctcct gattttcgtt
    16381 ctaacagttg actctcaact tgactagctg tttcttgctc gatttttttg cttcgtgaac
    16441 caaaaacttt cttccattct tcgattcgtg tcgttcccat tctcaatccc agatagccta
    16501 agatgatcat cagtaaaatc ggcaatacac tattgacgaa tggaatttcc atattgtaaa
    16561 gaggggtcga gatcacaaca cctaaagtca atccgatgat cgcaccgatc gcaccgaaga
    16621 gaagataagt caagctgacc tcacttagtt tttgttctac ttgtttgata atagctgaga
    16681 tatgtttggc caacagcaaa gataataaaa agaaaataag tgcgcctaac aggctgttgg
    16741 taaatgtatt attcagccat ccgtttgact gttgaccgat tgcctgccaa gctgagggta
    16801 aaaatgaaat acccaagctt gcgccgacaa tgatcatcag caaagtaatg acacgttttt
    16861 gcatcgtttt tcctccgttt cgacaatcat ttattagtta gcggaacaca cgcttcaacg
    16921 tttcagccaa agttgctact ccaactatct cgattccttt tggcgcttcc cagttaccta
    16981 agttgttttt aggtaggtat acttttgtga agccgagttt ttggacttct ttgactcgtt
    17041 gttcaatatg actgacacgt ctgatttctc cagtcagtcc aatttcaccg atgaaacatt
    17101 ccgttggctg tgttcctttt tctttatagc ttgaagcaat actgacagcg atagctaaat
    17161 caatcgctgg ttcatttaat tttaccccac ctgcagcttt taagtaagca tcttgattct
    17221 gaagaagcaa cccagctctt ttttccagca ctgccatgat cagagatacc cgattgaaat
    17281 caagacctgt tgttgttcgt ttggcatttc cgaacatagt aggtgtgact aatgcttgga
    17341 tctctaccaa aattggacgt gtcccttcca ttgccacaac gatagcagag ccagttgcac
    17401 cgtctaggcg ttcttctaag aaaatctgtg aaggattagc gacttcttcc aatccatgct
    17461 catgcatttc aaaaatccca atctcattcg ttgagccaaa tcggtttttt actgctcgca
    17521 aaatccgaaa actatgatgt tgttcacctt caaaatataa caccgtatcc accatatgtt
    17581 ctaacattct tgggccggcg atcgagcctt ctttggtaac atgaccgacg ataaaaatgg
    17641 ctatcccatt tgttttagcg attttgagta gttctgcagt ggtttctctt acttggctga
    17701 cacttcctgc cacacttgtg atgtctggct gcgtcatcgt ttggatggaa tcaatgataa
    17761 cataatcagg ggagatatgt tcgatggcac gactgatttc gttcatatct gtttcagcgt
    17821 atagataaaa ttctgtgttg atacttccga ggcgttccgc gcgtaatttt atctgctctg
    17881 cgctttcttc gccggataca tataagactt ttccgcctat ggctgctaat tgctgagaaa
    17941 cttgtaacag caaagtggat ttcccgatac ctggatcgcc accaatcaag accatagacc
    18001 ctggcaccac acctccgcct aacacacggt tcagttcttc taattttgtt ttgatccgag
    18061 gttctttttt agggacgacg tctgcgattt ttgttggttt tgctttttct cctgtcaaac
    18121 ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc ttcgaccatc gtattccatt
    18181 ttccgcaatt tgggcagcgt cccaagaact taggcgacac atacccgcaa ttttggcaaa
    18241 caaattgtac ttttgatttt tttgccattg taccgcctcc ttttatccta atcatgagac
    18301 attaaatcaa gcttaagtat ctttaacgtt ttatttactg gaagaaccaa atccgccttg
    18361 acgttgagca gtcgtttgat cttgatctgc ttttaaaaac ggcaaaaaga tcccttggcc
    18421 gatacgctcg ccttttttga tcactacatc cttgaaacca aagttggtga attggaacat
    18481 gatatggcct tcatttgcct cattattgta ataatcactg tcaatcacac ctactccgtt
    18541 tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg ttagcaagct gcaaaaattc
    18601 atcttctccc atgtaagctt tgattccagt aggcacgagg gttggtttca attgtttttc
    18661 attttctgat acatcttttt tcaatccatc tgcgactgtt tccaacccct ttttccagcc
    18721 tgctttccag atacttggaa tcacgacctc ttctgctgct tcaaaatcat agccagcagc
    18781 atgatgtgta gcgcgctgcg gtagattgat attttgatct ttatatttag aaataacttc
    18841 aaatcctctt tttctcaccg gttt
//
LOCUS       DAYYNG010000045        18822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000045 DAYYNG010000000
VERSION     DAYYNG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.045"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..535)
                     /locus_tag="ACHBFA_001254"
     CDS             complement(14..535)
                     /locus_tag="ACHBFA_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM9394759.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            734..4414
                     /gene="nifJ"
                     /locus_tag="ACHBFA_001255"
     CDS             734..4414
                     /gene="nifJ"
                     /locus_tag="ACHBFA_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM9394760.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYVQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            4603..5490
                     /gene="cdaA"
                     /locus_tag="ACHBFA_001256"
     CDS             4603..5490
                     /gene="cdaA"
                     /locus_tag="ACHBFA_001256"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9394761.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5487..6656
                     /locus_tag="ACHBFA_001257"
     CDS             5487..6656
                     /locus_tag="ACHBFA_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9394762.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6680..8035
                     /gene="glmM"
                     /locus_tag="ACHBFA_001258"
     CDS             6680..8035
                     /gene="glmM"
                     /locus_tag="ACHBFA_001258"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9394763.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8107..9912)
                     /gene="glmS"
                     /locus_tag="ACHBFA_001259"
     CDS             complement(8107..9912)
                     /gene="glmS"
                     /locus_tag="ACHBFA_001259"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9394764.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10322..10612
                     /locus_tag="ACHBFA_001260"
     CDS             10322..10612
                     /locus_tag="ACHBFA_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394765.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10625..11650
                     /gene="sppA"
                     /locus_tag="ACHBFA_001261"
     CDS             10625..11650
                     /gene="sppA"
                     /locus_tag="ACHBFA_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9394766.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11653..12312
                     /locus_tag="ACHBFA_001262"
     CDS             11653..12312
                     /locus_tag="ACHBFA_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9394767.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12469..13401
                     /locus_tag="ACHBFA_001263"
     CDS             12469..13401
                     /locus_tag="ACHBFA_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394768.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13555..14844
                     /gene="tig"
                     /locus_tag="ACHBFA_001264"
     CDS             13555..14844
                     /gene="tig"
                     /locus_tag="ACHBFA_001264"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9394769.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15091..16341
                     /gene="clpX"
                     /locus_tag="ACHBFA_001265"
     CDS             15091..16341
                     /gene="clpX"
                     /locus_tag="ACHBFA_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9394770.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16424..17011
                     /gene="yihA"
                     /locus_tag="ACHBFA_001266"
     CDS             16424..17011
                     /gene="yihA"
                     /locus_tag="ACHBFA_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9394771.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17085..17240)
                     /locus_tag="ACHBFA_001267"
     CDS             complement(17085..17240)
                     /locus_tag="ACHBFA_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9394772.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17376..18704
                     /locus_tag="ACHBFA_001268"
     CDS             17376..18704
                     /locus_tag="ACHBFA_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394773.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         6159 a         3342 c         4060 g         5261 t
ORIGIN      
        1 gctccttatt tttttatttt gtttggatca attcttttaa ttcctctatt gaaatagatt
       61 catcaaagat ttctttaccc aagatgacag ttggaacaaa tcgaatatgt gcttgctcag
      121 cttcgttgat gacagcttgg ctcagctgtt cgtctttttg ttctgtcagc tttagtttat
      181 ctactgcaaa atccgctact tcttggagag aaagatgttt ccactcgtct tgagtatcga
      241 atatttgttt gatctctttg atcgcttttt ggccatcgct gattgtgaga taccggtgca
      301 tcacgttccc tcgctgtaag ctttcttttt ctttatcgaa caatttgatc actcgctgca
      361 attgcccgct ttggactgct tcttctaata gctcataaga ttcttcaaac cattgtcggc
      421 aatacggaca agctaagttg atgaattcga ccattttttt aggagcattc gatgaaccat
      481 aaaggattcc tttttgtgta tttgttttcg ttgcatcaat cactgatata tccatgttaa
      541 aacacctctt tcgcttatag tttatacatg tctgacaaaa aaaggcaacg atttagactg
      601 tttgtctaaa aattagatga ccaattcaaa tagatacatg taaaatattt ttataaataa
      661 aagaaaaaaa tggcatgttt tacgttataa tggaacagat aataagagag aatatgaaca
      721 aggatgatga atcatgcgaa aaatgaaaac aatggatggc aatacggcag ccgcatatat
      781 ttcttatgca tttaccgaga ttgctgcaat atatccaata acacccagtt cgaccatggc
      841 tgaattagta gatcaatggt cagcagcagg aaaagaaaac gtattcggtc aaccagtaaa
      901 agtagtagaa atgcaatccg aagcaggagc agctggtgtg gtccatggat cattgaaaac
      961 tggtgcactg acaactacat ataccgcttc acaagggctg cttttgatga taccgaatat
     1021 gtacaagatt gctggggaac tactgccaag tgttttccac gttgctagtc gggcagtgac
     1081 gacaaatgcg ttgaatattt tcggagatca cggagacgtg atggctgcaa gacaaacagg
     1141 atttgctatg ttagctgaaa gcagtgtaca agaagtgatg gatttagcac ctgttgccca
     1201 tcttgcttct attgaaggag aagtcccttt tttgaatttc tttgatggat tccgtacgag
     1261 tcacgagatt caaaaaatcg aagttttgga atatgaagaa ttagctcctc tgatcaatca
     1321 ggagaaatta gcttcatttc gtaaacgagg gatgaatccc aatcatccga ctgttagtgg
     1381 tacgaaccag aatccagaca tccattttca acaacgggaa acgatcaacc cctattatga
     1441 taaattacct gaaatcgttc aaaaatatat gaaagagatc aaccgattac gaggaacttc
     1501 ttatgacttg gtgacttatt acggagcaga cgatgcggaa gaagtcattg tttctatggg
     1561 atcagttgca cagacgattg aacaaacgat tgattacttg aatgcaaacg gacggaaaac
     1621 aggttttttg aatatccatc tttatcgtcc ttttccagtg gaaacattcg tgcaaaagtt
     1681 gcctcataca gtaaaaacaa ttgctgttct tgatcgaagt aaagaaccag gggcaggtgg
     1741 agaacctctc ttattagacg ttcaaagtgc gttatatgat tctgaacttc gcccggctgt
     1801 tattggtggt cgctacggat tggggtctaa agatgtgact cccgaccaaa tctcagcagt
     1861 ttttgatgaa ttgaaaaaag acccaagcat acgcaaaaaa cggtttacca tagggatcgt
     1921 ggatgatgtg acttatcaat ctttggaacc aaaggaaagt ctggatttga cagaaccgca
     1981 aacattccag gccaagtttt ggggatttgg atctgacgga acagttggag caaataaatc
     2041 agctatcaag attattggag atcacaccga taaatatgcg caaggctatt tttattatga
     2101 ttcgaaaaaa tctggcggtc tgactgtctc tcatcttcgt tttggagata cgccgatacg
     2161 ttcagcgtat cttgtcgagc atgcggacct tgttgcctgt cacacaccag cttacctgca
     2221 tagctatgac ttggtcaaag gattgaagcc aggtggcatt tttttattga atactctttg
     2281 gagcgacgaa caattagaaa cgcatctgcc cttaaaatta aaacgttact tggctgaaaa
     2341 taacattaga ttctatacga tcaatgccat gagattggcc caagaagtcg gtttgggacg
     2401 ccggatcaat acggcaatgg aaacggcttt ttttaaatta gctgacatca ttccttttga
     2461 tgaggtgctt ccgctgctaa aagaagaagc cctgaaaagc tatggacata aatcaatggc
     2521 tatcgtagaa aaaaacatac aagcaatcga tcggacgacc gaactgcttc atcaagtcgc
     2581 gatcccagac cattggagaa cgttggagat gcctgaaaaa atcaataaaa ggaacactgg
     2641 ttatgtacaa gaaatacttg aaccagtgaa tgcacaggaa ggaaatcaac tctctgttgg
     2701 tacacttgta gcaaatggaa tgacaactgg cgaaatgcct ttaggaacgg cttctatgga
     2761 aaagcggggc gtcgctttag aagtacctga gtggattagt gatcgctgta cgatgtgtaa
     2821 tgaatgtgcc tttgtttgtc ctcatgctgc catccgtcca tttttagcag atgaagagga
     2881 aatggaagaa gcgccagaag gatttatcgt gagagaaatg cgaggcgcag acggcgtaaa
     2941 ataccgtatc caagtttcag tggaagactg tacaggttgt ggtctgtgtg tagaggcttg
     3001 tcccgcaaaa gaaaaagctt tagtgatgaa gccttatgaa gaacagaaag aacaagctgt
     3061 gaattgggct tttgcaatga cattaagaca aaaagcaaat ccagtcaaag gaaagaatac
     3121 tgtcttgtct acacaattca atcaaccgct tcttgaattc tcgggcgctt gctcaggctg
     3181 tggcgaaaca ccttatgtaa aattattgac acaaatgttc ggtgatcgaa tgatgatcgc
     3241 gaatgcgaca ggctgttctt ctatttgggg cggtgcttct cctgcttctc cttatacgac
     3301 aaatgaatgt ggtcaagggc ctgcatggtc aaattcttta ttggaagata atgctgaatt
     3361 tggttatggt atgcttctag cggcacagac tcgtcgcgag gctttggcaa gttcaatcaa
     3421 agaagtattg ccaaaagtat cagaagaact acgagaattg atggaagact ggttggtaca
     3481 catgtttgag agcgaaggaa cgcagcaaag agcagcaaaa ctgcaagctg cgatcgaaag
     3541 cgaacgatca attgtccctg aactggagga tatatacaag agaaaggatc tattcgtaaa
     3601 aaacagccaa tggatgattg gtggagacgg atgggcttac gacattggtt ttggcgggat
     3661 cgatcatgta cttgccagcg gagcagacgt gaatattctt gttttggata acgaagtcta
     3721 ttccaatact ggcggtcaaa cctcaaaagc aacaccagct tcggcaatcg ctaaattttc
     3781 ggctagtgga aagtatgctt caaagaaaga tctaggaatg atggcgatga catatggaaa
     3841 tgtttatgtg gcgcaaattg cttcaggtgc caatcaaatg cagacgataa aggcttttga
     3901 agaagcagaa aagtatcctg gaccttcttt aatcattgcc tatacgcctt gtatcacaca
     3961 tggtttgctt ggtggaatga aagaatcgct taaagaagca aaagaagctg tttcttctgg
     4021 ctattggtca ttatatcgat ataaccctgc aatggtcgaa aaaaacaaga acccaatgac
     4081 tttggactac aaaaaacctg atttctctgc tatgcctgat ttcatgagga aacaagttcg
     4141 cttttcctcg ctagagaacg caaatccaaa tcttgcaaaa gctttatttg aaaagactat
     4201 ccaagacgct aaaacacgtt tttacaacta tgctgcactg acgggagaat tagaaaaaat
     4261 caagaaacga cttgaaccag aggaatctat cgatgtaccc gttaagacta gaaaagaaaa
     4321 aacgacggac cttgaagcag aagaaagaag agctgccaga gcagaacgac gtaaaaaaat
     4381 ggaacaagct acaaaagaaa aaaaggaaaa ataacagaaa aaatataatg aataattctc
     4441 tgaagtttaa aaatatttat tgcagagaaa aataaaacgc atacttgcaa ttttcctgaa
     4501 aattatgcgt ttttctcaag aaaaatacga gcagatcagc ctctaaaatt aaaacatatg
     4561 atatactaga gaggaaatct caaaagagaa gagaggcttt ttatgtcatt tcaaatcggg
     4621 gaacttttta atttggacta ttggcagcaa atgttttccg gtgacttgtt ctctctgaat
     4681 tttttggtga acatacttga tattctggtt gtctggtatc ttgtatacaa actgatccaa
     4741 ctgctgcgag gcacaaaagc gattcagctt ctgaaaggtg tagctatttt tattgttatc
     4801 cgaatcgtag cggaaatcat tggtctgcac accttgtctt ggctgatgaa ccaagtcatt
     4861 acgtacgggg tgatagctgc tatcgtgatt tttcagccgg aaatccggag aggactggaa
     4921 catcttggac gcagttcgct tttcaaacaa tcaaaaagcg aacagcagga agatgagaag
     4981 atgatcttgt cttttgacaa agccatccaa tatatgtcca agcgaaaaat cggtgcgcta
     5041 attactatcg aacggcatac tggattagat gagtatattg agacaggaat tgcattagat
     5101 gcagatatca ctggagagct gctgatcaat atctttattc ctaatacacc gctgcatgac
     5161 ggtgcagtta ttgtaaaaga aggaaagatc gcagtagcaa gcgcctatct tcctctatca
     5221 gaaagtatgt tgattccaaa agaattcggg actcgacacc gggcagctgt cggtataagc
     5281 gaagtcagcg acgcgattac catcgttgtc tctgaagaaa ctggggatgt cagcattaca
     5341 ttagataatg aactgatggc tggcttatcc cagcaagaat atttggcaat tttgcgtaga
     5401 gaactgattg ctgaagagaa aacaaaaaac aagaaaaacg tgattcaatc tttcctagac
     5461 ggtatgacta agggagggga tcgcaaatga tctcaaaaga gaaacggaca agcattcttt
     5521 acagttttat tgcattgctg ttcagtattg cgctgtactt caatgcaaat ggccagtccg
     5581 ttccaaatac gctttctgga aatgaagcgt ataatcaaac agttactggg gtgccaatca
     5641 agatttctta tgattcaaag cgctactata tccatggata tgaaaatgca gtgactgtca
     5701 aactttccag tgcaaaccga gttcagttga atgcagaggc aaacgaagat acacggatgt
     5761 tccgcgtcac agcagatatc actaagctgg gcgaaggaac acatgaggtt ccgctaaaaa
     5821 ttcaaaattt gagcagtgct gtcacagcaa agatcgaacc atcgactatt acagtaacgg
     5881 ttgagaaaaa ggtaataaaa gatttcgatg tggaaacgtt gattccttca acgttcacac
     5941 catctggata tgaacttgat gatacttccg ttagtcctaa aaaagtctct atcactacag
     6001 gtgacaaaac attggcagaa atcaaacgag tagtagctga tgtggacgct tcgatggtca
     6061 cagatgatgg gattaatagt gaggtcccaa tacaggcgct gaatgcagca ggggaagtat
     6121 taagtatcgt ctctgatcca gtgcaagtaa cagtgagagc tgattcgatc aagccaagca
     6181 agtcggtacg actttatggg atacagcaag gaacaccagc tgccggggta gaaagctatg
     6241 atttcagttt ttcaaaactg gaagcagaag taagcggctc tagcgatttg cttgcatcga
     6301 tcggtgacag tattgctgta ccgatcaatg tctcaggtat caatcatcga acaactcgta
     6361 aaattgaaat accagtagaa gaggggttaa gtatcaagcc cgaatcagtt agtgtggaga
     6421 ttacacccgt gttgcaaacg tcaagcagta gctctgcttc gacgagcagt cggacacaag
     6481 aaaatacaag tacgagtaga agtatttcat ctgctgattc aggaacggaa ccatcgacaa
     6541 caaccatttc ttccagttcg gaatctggga caagcagctc gaccacaagt acagaagaaa
     6601 catcagaaac aagcgaatca acacaaatga gcagaacaaa ttcctcagaa aattaagtta
     6661 aaggagtaat tttacaacta tgggtaaata ttttggaaca gacggcgtac gcggtgaagc
     6721 caacaaggaa cttacgccag aacttgcatt taagctggga cggtgcggag gttatgtcct
     6781 aagccagcat gaagaagaag gaaaacgccc acgcgtgtta gtagggcggg atacaagaat
     6841 ctcaggtcaa atgttagaag aagcgctgat ttcaggactt ttatcggtgg gtatcgaagt
     6901 gttccagcta ggtgtgatct ctacaccagg tgttgcttat ctgacacgtt tgcaaaaagc
     6961 aagcgcaggg gtcatgattt ctgcttctca taatccagca caagataatg gaatcaaatt
     7021 tttcggtgca gatggtttta aactagtaga tgaccaagag ctagaaattg aagcattatt
     7081 agatgctgaa gtggatgaat tgcctcgtcc atcagctgaa ggattgggaa ctgtagaaga
     7141 atttccagaa ggcttattga aatattctca attccttgta caaacgatca acggtgacct
     7201 ttccggtttg accatttgtg tcgatgcagc aaacggagca acttcaacaa gtgttaatcg
     7261 tttgtttgcc gatcttgaaa cagattttta taccatgggc acaaatccag acgggttaaa
     7321 tatcaatgat ggcgtaggct ctacccatcc agaacgttta gctgagatgg tcgttgaaaa
     7381 aggagcagat gctggactag ctttcgatgg cgatggcgat cgtattattg cggtggatga
     7441 acttggaaac attgtcgacg gtgataaaat catgtttatc tgtgctaaat acttggcaga
     7501 aaaaaatcga ttgaaaaaag atacgatcgt tacgactgtc atgagtaact taggattcca
     7561 caaagcggtg gaaggaatcg gtttgaaaga tgtcgttaca caagttggtg atcgttacgt
     7621 tgtagaagaa atgcgcaaaa acgattataa ctttggcggc gagcagtctg ggcacatgat
     7681 tttcttagat tttaacacaa ctggagatgg gatgctttca ggaattcagt tactgaatat
     7741 catgaaacag acaggtaaga aattatcaga gcttgcagca gaggtaacaa tctatccgca
     7801 aaaattagtg aatatccgag taagtgataa aaacggtgcg atggatgttc cggcaatcaa
     7861 aaaagtgatt gaagaggcag aagctgaaat gggatcagaa ggaagaatcc tagttcgtcc
     7921 ttccggtaca gaaccattac ttcgagtgat ggcagaagcg cctactgatg agaaagttaa
     7981 ttactatgtc gataaaatcg cggcagtagt caaagaagaa atcggactgg attaagagat
     8041 attttcataa aaaataaaag aacaaggacc agtgtcagat gtctgacact ggtccttgtt
     8101 cttttattat tctactgtca ctgatttcgc taaattacgt ggtttgtcaa cgtcgaatcc
     8161 ccgtaatagc gttgcgtaat atgcaagtaa ttgagtcggt acgactgtgg ttagagtagt
     8221 tagataaggg tggacagttg gtagcaccag ctggtcactt tccttttcta atccctctga
     8281 gacaataact agtgttttag cgccacggct ttcgacttct ttcaggtttc ctcgagtatg
     8341 cgcaccagtc acttcttcac tgatgattcc aatcactggt gttccttctt cgatcaaagc
     8401 aatcgttcca tgtttcaatt cgccagcggc aaaaccttct gcctggatat aactgatttc
     8461 tttcaatttc aaagcagctt caagagaaac gttgtagtca gcgccacgtc caatgtaaaa
     8521 ggcatttctt gtatcgctga gatatacttc tgccaactct tctaaataat ctttttgggc
     8581 gatgaccgat tccatcgcat tagcaactaa actcaattca tgagtgatat cgaatacaag
     8641 agaagtttca aattcttttt tatcaccgat agctttggct aataaagtta gtacagctat
     8701 ttgtgcagta taagctttcg ttgatgctac tgcgatttcg ggtccagcat gcaatagcag
     8761 tgtaaaggat gcttcccgcg aaagagtaga accagcaaca tttgtaatcg taagtgaagg
     8821 gtagccgaga cggttgattt ttaccagaac ttggcggcta tctgctgttt cgccactttg
     8881 actcaagaaa ataaagaatg gtttttttga aagaagaggt gtgttatagc caaattcact
     8941 ggctaaatga acctcaacag ggatctgtgt caatttttcc agagtctgtt tacctgctaa
     9001 ccccgcatgg taactagttc cgcaagcaac gatatagatt cgatcgctat cagacaatgt
     9061 atcgaccagc ttttgatcaa gagagacacg attgtcttct gttacgtatt tttgagcgat
     9121 acgacgcatg ataataggct gttcgtcgat ttctttcagc atataaaaag gataggtacc
     9181 tttctcgata tctgaagaat ctacttgtgc ttcgaatgaa gaacgttcta cgatgtcacc
     9241 tgcaaaattt tgaataataa ctttttctga tgtgacggta accatctctt catcttcaat
     9301 ctcaacgaat tccttcgtat gagcaagcat agccattgca tcgctggcaa tcacattgaa
     9361 tccatcgccg agtccgatta atagtggact tttattttta gcgacgtaaa taacatcagg
     9421 tgctgtccga tcaatcaaag cgaaagcgta agaacctttg atgacccgca aagcttttaa
     9481 aaacgcctct tgcgttgtag tatccttcgc gaaagtttca attagatgag cgataatctc
     9541 agtatcggtc tcaccaataa agtggtcatt tgctaaataa gcaattttaa gttcttcaaa
     9601 gttttcgatt acgccattgt gaacgagcac gaagcgtcca ctttgggaag tatgtggatg
     9661 ggcgttttct tcagaaggtt gcccgtgagt tgcccagcgt gtatgaccaa tacctatatt
     9721 tcctgtagta tcaatattta ttttgttttt caaattttga attctgcctt gtgctttgac
     9781 cagataatca gttgtgccat ctgagacaaa gataccagct gaatcataac cacggtattc
     9841 taatttttct agaccgtcaa tcaatcctgg tgttacattt tttaatccaa tcattccaac
     9901 aattccgcac ataaaaaaca tcctactttc tataatgaat tcgtatgaat gcttccagct
     9961 gcaggaaaat ccttttgttt tatctttgca gatatttttt gtggattttc tgtagagtgc
    10021 agtccctagg gccatccgcc gaatctttcg ataaaccctc tcctcgtcat ctgtttacac
    10081 agacctggcg ctatcctaaa aggtataact atcctcctct tttgtttttt atctggaaac
    10141 atttaaaatc ataacctttt gcgaatgttc ttgtcaacaa aaaaagaata aaaaataaat
    10201 tggtatatat caaatgcttt gtcaaatgct ctttttatgt caaaaaataa tgggttttta
    10261 tctatcatct ttttcaggag ttcggtacaa tagaaagtaa tcggatagag gaggagtaaa
    10321 aatggattca aaaattgaga tcatgacact gggaatgtta aaaaaacaat tgtccaaatt
    10381 cgaagcatca gctggtgtat cagatgatac gaaaatattt ttagatactg gctgggatag
    10441 catccaagaa atcgcacctg atgccttgga agttgctgaa gctcgtgagt ttacggtaga
    10501 agatgaattg acaaaagagt cgttttccgg gtatgcgcgg gaagaaaaag cagagcggtt
    10561 cgacacttct gagcagtcag aaactgtgat cgtcataaaa aacttgtatt agaaaggaga
    10621 aagaatgaat aagagacgtt gggcagcagt tctgattgct gcaggattat ttgtggtttc
    10681 gttagtttca gctagtttga ctaagcctca ggaaaacgat caaattgagg gattgaacag
    10741 ttggttatat ggggatgaag aactagcttc aaatgtgttg gaagagggag acagtacgca
    10801 aaaaatcgta aaattatcag tagatggaac gattgctgcg ggagggtctt caggtttatt
    10861 tgcttctgaa ggttacaatc ataaaaattt tatggaacaa ctgaaggcga ttgaagaaga
    10921 tccaacagtc aaaggtatct ttttagaagt aaattctcct ggtggcggtg tctacgaaag
    10981 tgcggaaatc gctaagaaat tggatacgat tcgtaaagaa catgatatcc caatgtatgt
    11041 cagcatgaaa aacatggcag ctagcggtgg ctactatatt tctgctcaag cagataaaat
    11101 ctttgcaaca gaagaaacag tgaccggctc aattggtgtg atcatgtcag gattgaatta
    11161 ttcaggactt ctagaaaaac tgggtgtgga agatacaaca gttaaaagcg gagcgttgaa
    11221 agatatgggt tcaggaacgc gaccagagac aaaagaagaa gaagcggtgc ttcaagcata
    11281 tatcgacagc gcatatagtc gttttgtagc aatcgtcagc gagggacgga ataaatctga
    11341 agaagaagtc aaaaaaattg ctgatggaag gatttatgat ggtgtccaag caaaagaagt
    11401 aggtttagtc gatgaattgg gatttcctga agatgcttta aaagctatgc gaaaggaaca
    11461 acagcttgaa gatgcggaac tagtagaata ttcttccacg ccatctgggt ttgctagtac
    11521 atggttagga agtaaactag cagaattcca gggattgaag ccttctgaga ctagtcagat
    11581 cttatccgtt cttgagaatc tagggacagc ggaatcacca aaagcaatgt attattacgg
    11641 aggtgagtaa gcatgtcaga gctttcaaat gaaaataagg aacaagaaaa agataagcaa
    11701 cagcagcctg ctgtaggacc tgactccaac ttttcccaaa aggtactgag ctcttttgaa
    11761 agagagcctt tgacgcccga agaaatcaaa aagaaacaac gtaaatggaa aaagtatacg
    11821 gacacaagaa atgaaccgcc aaaagcaaat gattttccta agtatttcta tgcaggtttt
    11881 tttatccgaa tgtttgcttt cttggtcgat cttttgtgta tcggagctat tacgcgttta
    11941 acacttggta ttgccaaaaa tctaggttgg cttaattttt cagattctta tctgagtatc
    12001 tacggattca cagccttact tatttatcta gcgtatttta ttttattgac gaaattcaat
    12061 catggtcaaa cgattgggaa aatgatcttt ggtattcgag tcatcagctt tgatgaagaa
    12121 gaacttagct ggtcaaccgt tttagttaga gaaggtgctt gccggttcat tctaaaattt
    12181 cctttgctga tgttagggta tcttccagca atctttaatc agaaaaagca acatctaggc
    12241 gattttttca ctaatacaag tgttgttacg atcaatctga tcaaagcatt caaccaggaa
    12301 gtcaacagct gatgagcaat aaaaagcagt aacagttgaa agcaccttct aaaagtcaga
    12361 tctaaaatct agcttttaga gggtgcttca gttattttta attgtcatgc tttttttgtt
    12421 ttgctactat tgaacatgag ctgaacatga gcaagaggag gacactgcgt gaagccgatt
    12481 gattttccag aaaaatatga gaatttaatg cgagtagcac aacaagcact agcaaaccag
    12541 caatatcagc aggcaaaaga attatttcaa cgagcttacg agttgaaaga aagctttgag
    12601 gctaatagcc tgcttgtttt ttgtctttac gagttggacg agaaaaaaga agcattgaag
    12661 caagcactct tgcatgaaaa acagtatctt caaaatgagg agtttgctga attttatttt
    12721 gacttgttaa tcagtacaca agatttttta tatgctagaa aattgatagc aagtacagat
    12781 ttttatgaag ctttcgagca gcgggtcatt gaaaaaatcc aatttgcaga agaattatcc
    12841 ggacaaatgg agaggcagaa gatcaaggca ctccacaata aatcggagga acttccttcg
    12901 ttggaacctg cacgtcagct tgcgtctatt gatgagatcg aacagcttcc ttatcatgag
    12961 tttatccaaa cagcgagtaa acttattgta tcgccagagg tccatatttt agcaagggca
    13021 aaattactgg agacacttag gcaattgaac gaatgccatc ctgttttcta tttgacgatt
    13081 gaagaaaagt tagtcaaagt cataccaaaa gatctgccaa agccgcagca gcaggcaagt
    13141 taccagcaat tatgtgtatt ttcagatcgt tacggtaatg aagatgcgct gcttagttcc
    13201 gtgttaaatg aagaattcac gttgcaatct gcaattgttt atcctgtcta tgatacatac
    13261 atcgaagacc cgaagcgctg gtttcaactg actgtagagg cgtgtacagg aaaaacgatg
    13321 tatgaaggta cagaagatga aaaacaagat tttttaaaaa aaagagaaaa aattttgcag
    13381 caaatgatct tcttccatta gattttatca gtgaaaaaga taaaaatgtt atgaaaaaca
    13441 aggattctat ccattcaata tgtttacaat ttgaaagggt tagtgtacta tttaaaggta
    13501 tgcatgaaac gtttttgatg tataaaatga atttctaaac tcggagggaa ttaaatgact
    13561 gcaacatttg aaaaaaaagg cacgaatgat ggtgttttaa aatttacgat tgcgcaagac
    13621 gaaatccaaa aaggattaac aactgcgttt aataaagtga aaaaaagctt gaacgtacct
    13681 ggattccgta aagggaaagt ttctcgtcaa gtattcaacc gtatgtacgg tgaagaagct
    13741 ttatatgaag atgcattgaa tgctgtatta ccagaagctt atgaagctgc tgtcaaagaa
    13801 gctggcatcg atcctgtttc tcaaccaaaa atcgatgttg aaagcatgga aaaaggtcaa
    13861 gactgggtaa tcactgctga agtaacagtg aaaccagaag taaaattagg acaatataaa
    13921 gatctagttg ttgaaaaaca agaccgtgaa gtaacagatg ctgacgtgga tgcacgtatc
    13981 aaacgtgaac aagaagcaca agctgaatta gttatcaaag aagatgctgc agctgaaaat
    14041 ggcgatacag tagtaatcga cttcgaaggc tttgtagatg gcgaagcgtt tgatggcgga
    14101 aaaggcgaaa attactcact tgaattaggt tcaggctcat tcattccagg ttttgaagat
    14161 caattagttg gtcataaatc aggtgaaagt ctagatgtaa atgtaacttt cccagaagat
    14221 tatcaagcag aagatcttgc tggaaaagaa gcagtcttca aagtgacaat ccatgaagta
    14281 aaagcaaaag aagtgcctga attagatgac gaattcgcaa aagatgttga tgacgaagta
    14341 tcaacattag acgaattgaa agaaaaatat cgcaaagaat tagtagaaac aaaagaaaaa
    14401 gcagcggacg atgcaaaaga tgaagcagcg atccgtatgg ctgttgaaaa cgcagaaatc
    14461 gttgaattgc cacacgtgat ggttcatgat gaagtacatc gttcaatgga cgaattcttg
    14521 aacaacatgc aacgtcaagg tatctctcct gaaatgtact atcaattaac tggttctaca
    14581 gaagaagact tgcataaaca attcgaaggt gaagcagaaa tgcgcacaag aacaaatctt
    14641 gtgatcgaag caattgcagc tgctgaaaat cttcaagcaa cagaagaaga aatcaatgca
    14701 gaaatccaag aattatcaga aacatacaac atgccaatcg aacaaatcaa acgcgtgttg
    14761 acagaagata tgctggaaca tgatgtgaca atgaaaaaag caatcgaatt gatcacaggt
    14821 acagcgacag aagcgaaaga atagaaatca agtaataaat catttctgaa gaagctactt
    14881 ctagaaaatt gtttattcga aataagggac tgtgacacga ttttgtcaca gttccttatt
    14941 ttttgttaag agtaaaaaat gcaaaggtat aggtcagttg atcctttgtt aaacactttt
    15001 tctttttact aaactatgtt atgatagtaa aagactgtaa agtctttgat agtttcttgc
    15061 atactttttg caagttaagg ggtgaagacc atgtatgaca atccaagtag caatgagacc
    15121 gtacgctgtt ctttttgcgg gaaaacccaa gaagaagtga aaaagatcgt agctggtcca
    15181 ggggtctaca tctgtaatga atgtatcgac ctatgtaaag aaatcattga tgaagaattt
    15241 tatgaagaag ctgtacgcga attcactgaa gtccctaaac cactagaaat tcttgaagta
    15301 ttgaataact acgtgattgg acaagatcgc gcaaaacgct cgttagctgt tgccgtatac
    15361 aatcactata aacgtgtcaa tcagcaagca caagaaaaca cagatgatgt tgaactgcaa
    15421 aaaagcaata tctgcttgat cggacctaca gggtctggta aaacattttt agcacaaaca
    15481 ttagcaaaaa cattgaatgt accttttgca atcgcagatg cgacaagttt gacagaagct
    15541 ggttatgtgg gggaagatgt agaaaacatt ttgcttaaat tgcttcaatc tgctgattac
    15601 aatgtggaac gtgcagaaaa agggatcatc tatattgatg agatcgataa aattgcgcgt
    15661 aaaagcgaaa atgtatctat tactcgtgac gtttccggcg aaggtgtcca acaagccttg
    15721 ctgaaaattc tagaaggaac tgttgcaagc gtgccacctc agggaggacg taaacatcca
    15781 catcaagaat ttattcagat tgatacgacg aacatattgt tcatcgtagg tggtgcattt
    15841 gatggcatcg aaacgattgt gaagaatcgt atgggcgaaa agacgattgg atttggtaca
    15901 caaaaccaaa aactttctga agacgaaagt gtcatgcagc atatcattcc tgaagattta
    15961 ctaaaattcg gattgatccc tgagtttatc ggacgattac ctgttatggc agctttagag
    16021 aaattgacaa cagatgatct ggtacggatc ttgactgagc cgaaaaatgc gctagtcaaa
    16081 caatatcaaa aacttctttc tctagataat accgaacttg aattcgaacc tgaagcgttg
    16141 agagccattg cgaaaaaagc aatcgaacgc aataccggtg ctcgaggact tcgttccatc
    16201 attgaagaaa tcatgatgga tgtgatgttt gatatccctt caaatgaaaa catcgaaaaa
    16261 gtgattatca caaaagaagc agctgaattg tctggtgagc caacagtaat ctataaagat
    16321 tcagagaaaa aagcgggttg atgaaaagag gctgccgtaa aagtcatttt ggcttttgcg
    16381 acagccgctt gctttattca tcaccttgtt aaggagagag accatgaaag tccatcaagc
    16441 tgaaattatc ataagtgcag tggcaccaaa acaatatcca gatacacaat tgccagaaat
    16501 agctctggca ggacgatcaa atgtagggaa atcttctttt atcaacacat tgattgatcg
    16561 aaagaattta gcccgtactt ccggtaagcc aggaaagacc cagacattga atttttattt
    16621 gatcgagaac gcattacatt ttgtcgatgt tccgggatat gggtatgcaa aagtatccaa
    16681 aactgagcga gcaaaatggg gaaagatgat cgagacatat ttaacgacta gagaccagct
    16741 acgtgcggtc gtttctttag tagatttgcg tcataagcct tctcaagaag atatccagat
    16801 gtatcaattt ttgaagtatt atgatatccc tgtaattgtt gttgccacta aggcagataa
    16861 gatccctcgt ggtaaatgga acaagcatga gagcgaaatc aaaaaagcgt tggattttga
    16921 tcgcaatgac acgtttattc tcttttcttc cgttacaaaa cagggaaaag aagaagcgtg
    16981 ggcagcaatc gaacaagcaa ttgaatcata aaaagaataa aaaagactta ccagtcaaac
    17041 tctttcataa aagtttggct ggtaagtctt tttttgagct taacttactt ttgtttcttg
    17101 tcttttttct tttcattttg tggctgtggt tttagaaatc gatcgatctc ttcttttggt
    17161 gcaactgttt tttccggatc gacagcaaat gattcgaaaa gtttatctaa attatctgta
    17221 cgttcaaatt tcaaccccat tgtaaacacc tctttgtttc tttttactta gtctatcatg
    17281 taatcgtcca aattggtttc taagacataa agttttagtt tttctttatc gttggttata
    17341 attaaattat aaaaagattt taaagaggtg agaaaatgat tggcgaagat aaattattga
    17401 tgactatcca gctcacagac ttaaatgaag aagaaaaaaa acaggaaata agcgattggg
    17461 ctattttgac acgtctgttg atccagccaa catttatccg ctctttcaca caacaagcgg
    17521 atccgtatga tcttcgaaag cttcaagaaa aaataatgct agcatcggta agagacgaaa
    17581 gttggttagt tgtaggaaat gacgagaacg aatgtagttt tcgcttggaa gataatcaat
    17641 tattgataaa aaatgtattg tctatctctg ttttcaaaga aaagcaattt ttgattcgag
    17701 acttcattca gaaaaaaatg attgctcatg gggtgttcgc ctatatgcga gcttatagcg
    17761 agtttatcta tcacaataca aaacagatct ctcaacggct attgtttgag aaaaaggatg
    17821 aaatcgaaca tttacctaaa atgaaacaac aaaacgggga agtagtagtc gattgcaacc
    17881 aatttccagg atacgatctt ttttaccaag gtttatgttt cacttcgtgt tgggaaatgt
    17941 attatagtcg ctattatcat caaatcatcc ctaaaccgat ttttctcgac atccaacaag
    18001 tagaaaaagt gaaggaatta gaaaacgaag tcatatgtat ccagctttat cgagacccct
    18061 ttaactggca aaagccaaac aatcaaatgt ttcaatccta ttttagggac cagcttgggt
    18121 ttgatcatct tgcttgggac aatggtgtcg gtttattgaa accgccattc gttgaataca
    18181 tttacactga ccatacgatc caatctgtcc agtatcagaa tgcacaaatg caacctgttc
    18241 ctaaaaagaa tgcttcattt tttgtaacaa aaagttatga tattcaacaa ggagattata
    18301 aagaaaggcg tgttcgcgga acactaaatg ctcaggcgta ttttccttgg gttgacgaga
    18361 atcgctcgcg catgatgtgc tataaagtta tcgatccgac tgttgctttg gataatggaa
    18421 tcgaagctta ttgttattat attagggaat atctagaagt agaggtaaca gatgaaaaat
    18481 atcaagagta tcttttatcc ttaagaatct atgttccttc agaatccctg tcagaattac
    18541 cgttaaagga aatcaaacat agactctctg atgttacctt caaacgaata aagaaaaagc
    18601 ggcgaagtat ccaattcgat gtcaagaaag gtgagcagca tctgcgtgtg caatttttag
    18661 attaccgaga attagaacaa ttgattactt tacaaaaaat atgaggtgaa gaaaatggat
    18721 aaacaactct attatcgaat aatggttccg gcatcattat tatccgtctg tctctttttt
    18781 gttcctaatt ttcctattcg atcagtcatc ggttttttgg tc
//
LOCUS       DAYYNG010000046        18686 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000046 DAYYNG010000000
VERSION     DAYYNG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.046"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            247..948
                     /locus_tag="ACHBFA_001269"
     CDS             247..948
                     /locus_tag="ACHBFA_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9394774.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1557..2573)
                     /gene="tsaD"
                     /locus_tag="ACHBFA_001270"
     CDS             complement(1557..2573)
                     /gene="tsaD"
                     /locus_tag="ACHBFA_001270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9394775.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2570..3034)
                     /gene="rimI"
                     /locus_tag="ACHBFA_001271"
     CDS             complement(2570..3034)
                     /gene="rimI"
                     /locus_tag="ACHBFA_001271"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9394776.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3041..3583)
                     /gene="rimI"
                     /locus_tag="ACHBFA_001272"
     CDS             complement(3041..3583)
                     /gene="rimI"
                     /locus_tag="ACHBFA_001272"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9394777.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3567..4289)
                     /gene="tsaB"
                     /locus_tag="ACHBFA_001273"
     CDS             complement(3567..4289)
                     /gene="tsaB"
                     /locus_tag="ACHBFA_001273"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9394778.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4480..5460)
                     /locus_tag="ACHBFA_001274"
     CDS             complement(4480..5460)
                     /locus_tag="ACHBFA_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9394779.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5484..6968)
                     /gene="galT"
                     /locus_tag="ACHBFA_001275"
     CDS             complement(5484..6968)
                     /gene="galT"
                     /locus_tag="ACHBFA_001275"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9394780.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6980..7969)
                     /gene="galE"
                     /locus_tag="ACHBFA_001276"
     CDS             complement(6980..7969)
                     /gene="galE"
                     /locus_tag="ACHBFA_001276"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9394781.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8217..8384
                     /locus_tag="ACHBFA_001277"
     CDS             8217..8384
                     /locus_tag="ACHBFA_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9394782.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8446..10257)
                     /locus_tag="ACHBFA_001278"
     CDS             complement(8446..10257)
                     /locus_tag="ACHBFA_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM9394783.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10254..10619)
                     /locus_tag="ACHBFA_001279"
     CDS             complement(10254..10619)
                     /locus_tag="ACHBFA_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM9394784.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10782..11177)
                     /locus_tag="ACHBFA_001280"
     CDS             complement(10782..11177)
                     /locus_tag="ACHBFA_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9394785.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11195..12157)
                     /locus_tag="ACHBFA_001281"
     CDS             complement(11195..12157)
                     /locus_tag="ACHBFA_001281"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9394786.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12157..12369)
                     /locus_tag="ACHBFA_001282"
     CDS             complement(12157..12369)
                     /locus_tag="ACHBFA_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9394787.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12390..13088)
                     /locus_tag="ACHBFA_001283"
     CDS             complement(12390..13088)
                     /locus_tag="ACHBFA_001283"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9394788.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13108..13650)
                     /locus_tag="ACHBFA_001284"
     CDS             complement(13108..13650)
                     /locus_tag="ACHBFA_001284"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9394789.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13782..14786)
                     /locus_tag="ACHBFA_001285"
     CDS             complement(13782..14786)
                     /locus_tag="ACHBFA_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9394790.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14783..15772)
                     /locus_tag="ACHBFA_001286"
     CDS             complement(14783..15772)
                     /locus_tag="ACHBFA_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9394791.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15769..16575)
                     /locus_tag="ACHBFA_001287"
     CDS             complement(15769..16575)
                     /locus_tag="ACHBFA_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394792.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16741..17697
                     /locus_tag="ACHBFA_001288"
     CDS             16741..17697
                     /locus_tag="ACHBFA_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9394793.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17774..18292)
                     /locus_tag="ACHBFA_001289"
     CDS             complement(17774..18292)
                     /locus_tag="ACHBFA_001289"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9394794.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18380..18529)
                     /gene="rpmG"
                     /locus_tag="ACHBFA_001290"
     CDS             complement(18380..18529)
                     /gene="rpmG"
                     /locus_tag="ACHBFA_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9394795.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         5596 a         4025 c         3218 g         5847 t
ORIGIN      
        1 aataaattaa aaaaataaat tatattatcg ttgttagcgt ttactatatg tattattgtt
       61 gttttttata cacaaatcat tcgcttcaaa acaaattatt caataccatt gtatattttt
      121 tacataataa caaattatat ttgtaaagta ttaaaacttg tttctaatgt tggtatgtta
      181 aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac
      241 tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat
      301 tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct
      361 tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg
      421 accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac
      481 tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact
      541 tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa
      601 aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta
      661 gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta
      721 aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc
      781 accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa
      841 actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta tgacaaaaag
      901 cgttttactt tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa
      961 taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta aaacttctga
     1021 aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt gtgtttcagt
     1081 tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa gcgtcttgtc
     1141 ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca gtgagggcaa
     1201 tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca ataatgatta
     1261 aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt cgattaaacg
     1321 gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg aggagctgtt
     1381 ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact cttgattaaa
     1441 cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa catgagaagc
     1501 cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca acctctttat
     1561 tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt tcttttttgc
     1621 atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag tcgaagaggg
     1681 ggaatcacta gttctacctc agggagttta gttgccaaag catcctgcaa tccttcacgc
     1741 aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg ataattttga
     1801 caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa gctagctgct
     1861 aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa attgataaat
     1921 gcacttttta atccgctaaa actgaaatca taattatctt catggatcat cgctcgcgga
     1981 aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc gcttgggtaa
     2041 gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc atctcttgtc
     2101 tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt atgtcctccg
     2161 ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc tgcatagata
     2221 tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc aaaagcctta
     2281 gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt gacagcgact
     2341 gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa gcataaggtg
     2401 atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa acgtttatga
     2461 ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct aacgaccgct
     2521 acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt catttcatcg
     2581 tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg cgataatatg
     2641 atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt gctgcttcat
     2701 tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc gtaatcagta
     2761 attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca gcaatatttg
     2821 tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc tggttatctt
     2881 cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac tgcattttcg
     2941 tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat tctgccaagt
     3001 acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt tctaaaagtt
     3061 ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg atcgttgttg
     3121 cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata cgcacttcta
     3181 atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca ataagcagcg
     3241 aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata tggcaatcta
     3301 aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag atacctagat
     3361 atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc catggcaatt
     3421 cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct gtagtcaatt
     3481 ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa atcattttat
     3541 cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc gtctccaggt
     3601 gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg aggaagaaaa
     3661 gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt tacaccactt
     3721 ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc ctttatttca
     3781 gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag cttttgtagt
     3841 tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc taatctattt
     3901 tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa catgggcaca
     3961 ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga tactcctact
     4021 agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat acgaagccct
     4081 gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg acttatttga
     4141 agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt gcgtttgata
     4201 tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc caaagtttgg
     4261 tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat gacattctcg
     4321 ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc aaaactagta
     4381 caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa cacaaaaaag
     4441 ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg attctcgaat
     4501 aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat tacttaattg
     4561 aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta cagtagaaag
     4621 cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag aaatatcttc
     4681 tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta atgcatcatt
     4741 agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatactgct tcactgcttg
     4801 atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt agcctttttc
     4861 tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat ggtttttctt
     4921 cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga caaagtgttc
     4981 aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc cttgagaagt
     5041 gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta ataactgagc
     5101 ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa tggaatccca
     5161 cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc ctaggcgaat
     5221 agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga tcaggcgaag
     5281 cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc gttctgctgc
     5341 ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat agttgagaac
     5401 tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa ttgttgccat
     5461 ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa cgctgcctgt
     5521 cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa aacctgacct
     5581 acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg ttcttttttt
     5641 aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc taataagaaa
     5701 tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag ccccattact
     5761 tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg atgaaaaatc
     5821 ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa ctcaaagtaa
     5881 ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc tgctgaaaaa
     5941 gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac tgagttagct
     6001 gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat cggccatttc
     6061 acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc tatcggtgct
     6121 tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag aatcgatcct
     6181 ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac ttcagtaatc
     6241 cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg ttctgataaa
     6301 atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca gctttcaccg
     6361 tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa gcgacctcta
     6421 tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac cgcctcgcgt
     6481 tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt gatttccagt
     6541 tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct tgttttgatg
     6601 tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg gcttttcgaa
     6661 tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa atccattaat
     6721 tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt ttctcttgcc
     6781 ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct tacttcaatg
     6841 ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata taagcggtcc
     6901 atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat cgtttgactg
     6961 attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca tgccaatccc
     7021 atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc agaatctctc
     7081 tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt ggtgctattt
     7141 ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc ttgactgagt
     7201 atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc aagtattcca
     7261 aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg atacatgttc
     7321 catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga ccaagagcta
     7381 cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg atagatgcat
     7441 ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg ccatatgctt
     7501 ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac ggatttttag
     7561 gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa gttgctgcag
     7621 tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt aagatttgca
     7681 taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct cctactaaag
     7741 agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg gaacgtagaa
     7801 attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact gcttgtttgt
     7861 gacctgtgag caaattatct accacagcta cttgataccc cttttgaact agctggtcta
     7921 cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata aaaaacactc
     7981 ctttgattag taaaattgtg tttgttgatt ctatataatt taaaaaacga agtttacttt
     8041 tatggtgaaa acaaacaaag taaaatttcg taaattcata tggtaaattt ataataaaac
     8101 atttagtaaa cattttcaag gattttttgc aaaggtttac aaacaaacgt aaaactagcc
     8161 tttttttctc ttttgttcca taatagatat aacaaataat aaatgaggtg gattcaatga
     8221 aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc gttgctggtt
     8281 tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg
     8341 aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata
     8401 cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa
     8461 tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc
     8521 tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga
     8581 ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc
     8641 tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt
     8701 caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc
     8761 aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt
     8821 ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc
     8881 taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa
     8941 ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg
     9001 ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca
     9061 attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa
     9121 gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat
     9181 tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa
     9241 atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc
     9301 aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca
     9361 ttgctccagg ttttcatttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg
     9421 atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc
     9481 cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga
     9541 tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt
     9601 ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag
     9661 taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc
     9721 ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa
     9781 aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga
     9841 agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca
     9901 tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt
     9961 tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt
    10021 gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg
    10081 ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc
    10141 aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag
    10201 aatcctttct gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg
    10261 catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt
    10321 gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata
    10381 gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca
    10441 aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc
    10501 aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg
    10561 agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata
    10621 gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata
    10681 aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa
    10741 tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg
    10801 atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct
    10861 ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca
    10921 ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt
    10981 gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct
    11041 tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt
    11101 actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta
    11161 attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg
    11221 cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc
    11281 ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc
    11341 ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt
    11401 ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat
    11461 cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca
    11521 ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca
    11581 cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc
    11641 cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca
    11701 aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt
    11761 ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat
    11821 tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt
    11881 ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg
    11941 gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat
    12001 gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat
    12061 cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg
    12121 ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct
    12181 ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc
    12241 aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat
    12301 gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa
    12361 gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc
    12421 agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt
    12481 atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca
    12541 attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat
    12601 gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta
    12661 aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca
    12721 ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg
    12781 aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata
    12841 aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt
    12901 gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat tccggcataa
    12961 taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct
    13021 aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga
    13081 taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc
    13141 aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt
    13201 tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc
    13261 ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag
    13321 cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc
    13381 catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg
    13441 ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt
    13501 cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg
    13561 ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt
    13621 ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg
    13681 tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa
    13741 agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt
    13801 ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt
    13861 cctgtaggta cttccccact tagtagaatc actctagcaa ccatatctgc actaagcaga
    13921 aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact
    13981 agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca
    14041 ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta
    14101 aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa
    14161 agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag
    14221 ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat
    14281 aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac
    14341 aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag
    14401 caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag
    14461 tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat
    14521 atactagaat ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca
    14581 agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc
    14641 agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata
    14701 aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa
    14761 tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg
    14821 gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc
    14881 tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa
    14941 cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa
    15001 atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa
    15061 caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat
    15121 gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag
    15181 gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag
    15241 caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag
    15301 aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact
    15361 gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca
    15421 cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac
    15481 ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcact ccttgcatca
    15541 atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac
    15601 gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc
    15661 aaaaatctgc ttttcccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca
    15721 aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc
    15781 ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag
    15841 cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc
    15901 ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa
    15961 acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt
    16021 gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg
    16081 ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg
    16141 ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc
    16201 atccaacgcc cactcgattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg
    16261 ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact
    16321 agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc
    16381 agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa
    16441 taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat
    16501 cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc
    16561 tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt
    16621 ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt
    16681 tcgaaatatc aggaataata accaatgaga aagattctca attatgttag gaggaaagaa
    16741 atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg
    16801 agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca
    16861 tccaatagta ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg
    16921 cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct
    16981 atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag
    17041 gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat
    17101 ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa
    17161 atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa
    17221 gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa agatcatttc cgattatcaa
    17281 aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca
    17341 gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga
    17401 atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa
    17461 gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac
    17521 atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat
    17581 cttaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat
    17641 aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt
    17701 taactaaaaa aagcatcttc acgattagtc gtgatatgct ttttttctta ttaacaaact
    17761 agacgcatag attttattct tcttttgaag caaccctatt ttttactctt tcccattttt
    17821 gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg
    17881 cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca
    17941 atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg
    18001 aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca
    18061 ttacttccgc attttctttc gtgcgaaaat ttggctgcgt tgtcaacacg atgatatggc
    18121 gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc
    18181 gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac
    18241 catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg
    18301 ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga
    18361 attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg
    18421 ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat
    18481 agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc
    18541 ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac catgattttt
    18601 tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa
    18661 cggttttact tctcaaacaa aatctt
//
LOCUS       DAYYNG010000047        18263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000047 DAYYNG010000000
VERSION     DAYYNG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.047"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(776..2407)
                     /locus_tag="ACHBFA_001291"
     CDS             complement(776..2407)
                     /locus_tag="ACHBFA_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM9394796.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2421..4094)
                     /locus_tag="ACHBFA_001292"
     CDS             complement(2421..4094)
                     /locus_tag="ACHBFA_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM9394797.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4107..4424)
                     /locus_tag="ACHBFA_001293"
     CDS             complement(4107..4424)
                     /locus_tag="ACHBFA_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9394798.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4449..5849)
                     /gene="lacG"
                     /locus_tag="ACHBFA_001294"
     CDS             complement(4449..5849)
                     /gene="lacG"
                     /locus_tag="ACHBFA_001294"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9394799.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6086..6871
                     /locus_tag="ACHBFA_001295"
     CDS             6086..6871
                     /locus_tag="ACHBFA_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9394800.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6874..7566
                     /locus_tag="ACHBFA_001296"
     CDS             6874..7566
                     /locus_tag="ACHBFA_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9394801.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7840..8211)
                     /locus_tag="ACHBFA_001297"
     CDS             complement(7840..8211)
                     /locus_tag="ACHBFA_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM9394802.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8309..9922)
                     /locus_tag="ACHBFA_001298"
     CDS             complement(8309..9922)
                     /locus_tag="ACHBFA_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9394803.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEVGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKILREEKVPTSSWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAQIKGILKSEVYIGNSVHNRQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIEKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYACSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKASALKKAENRQNEIDCLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITDLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            complement(10034..10244)
                     /locus_tag="ACHBFA_001299"
                     /pseudo
     CDS             complement(10034..10244)
                     /locus_tag="ACHBFA_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            complement(10963..12177)
                     /locus_tag="ACHBFA_001300"
     CDS             complement(10963..12177)
                     /locus_tag="ACHBFA_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9394804.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRIAVACGISREYYNRIEKGKQP
                     LNNELKEIIEKQIERFNPREPLFLLIDYFRVRFPTTDALKIIRDVLQLKADYMLYEDF
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLGCMT
                     AGGKLKRLDLAINDKAGILDIPKLIEKYKAGECISYFRKQKDYGGTEKNGYDTPQNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKMNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDKENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTVVPQENDGIF"
     gene            complement(12796..13263)
                     /gene="ribE"
                     /locus_tag="ACHBFA_001301"
     CDS             complement(12796..13263)
                     /gene="ribE"
                     /locus_tag="ACHBFA_001301"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFM9394805.1"
                     /translation="MNTFEGNLVAENIKIGIVVARFNEFITSKLLAGALDNLKRENVD
                     EKDIEVAWVPGAFEIPMIASKMVKSKKYDAIICLGAVIRGSTSHYDYVCSEVSKGIAQ
                     ISLNTETPVMFGVLTTDTIEQAIERAGSKAGNKGSECAQGAIEMVNLIRALEV"
     gene            complement(13264..14469)
                     /locus_tag="ACHBFA_001302"
     CDS             complement(13264..14469)
                     /locus_tag="ACHBFA_001302"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFM9394806.1"
                     /translation="MKYKKIKEALQALQEGKLVLVMDDKERENEGDLICSAQFATTEN
                     VNFMATYAKGLICMPMSESLANRLMLSPMVENNTDNHETAFTVSIDYKDTTTGISAEE
                     RGLTARMCVVEDINPFDFRRPGHMFPLIAKNGGVLERNGHTEATIDLLRLAGLKECGL
                     CCEIMNENGKMMRTSDLIQFSQIHHIPTLTIKELQEYRKVYDMLVECVSVVEMPTKYG
                     DFKAHCYINKLNGEHHVALVMGELNSGNDVLCRVHSECLTGDVFGSLRCDCGQQLDRA
                     MKMIAENGSGVLLYMRQEGRGIGLVNKLKAYHLQDKGMDTLDANLALGFQGDLREYYI
                     GAQILRDLGIKSLHLLTNNPDKVYQLEDYGMQISSRVPIEIKANPYDSFYLKTKKDRM
                     GHILNMEEK"
     gene            complement(14489..15124)
                     /gene="ribE"
                     /locus_tag="ACHBFA_001303"
     CDS             complement(14489..15124)
                     /gene="ribE"
                     /locus_tag="ACHBFA_001303"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFM9394807.1"
                     /translation="MFTGIIEEIGKVERIQKDTYNCKLSIKSSKILEDIHLGDSIAVN
                     GICLTVTCFTRQSFIVDVMNETWNRTALNLLKHGSLVNLERAMHMNGRLGGHIVTGHI
                     DGTGRISSVRRDKNAVWYQINTQREILNLIVEKGSVTIDGISLTVAKVSKADFSVSVI
                     PHTLEQTILKNKQVGDMVNLENDILGKYVKKLIGNSNETELSKELLCRYGF"
     gene            complement(15109..16209)
                     /gene="ribD"
                     /locus_tag="ACHBFA_001304"
     CDS             complement(15109..16209)
                     /gene="ribD"
                     /locus_tag="ACHBFA_001304"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFM9394808.1"
                     /translation="MSDIEYMKIAIELAKKGAGYVNPNPMVGAVIVKNNRLIGQGYHE
                     VFGGLHAERNALKNCRESPVGATLYVTLEPCCHYGKTPPCTETIIKSGITRVVVGTLD
                     CNPIVSGKGVKQLEENNIQVDVGILEIECQQLIKVFRKYITKRIPYVFMKYAMTMDGK
                     IATYTNQSKWISGEKARKQVHQFRHKVTAIMVGVNTVIQDNPLLTCRLENGKNPIRIV
                     CDTDLRTPITSKIIQTANDIKTYIATSSIDESKIALYRKCGCEIIYTKKKGNHIDLMN
                     LMQCLGNMQIDSLLLEGGSALNWSALEQQIVDEVQTYIAPKIFGGSGKSPVSGQGVVF
                     PKDAIMLKPYAFSQVGNDYLIESEVIYPCLQE"
     regulatory      complement(16345..16459)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16933..17163)
                     /locus_tag="ACHBFA_001305"
     CDS             complement(16933..17163)
                     /locus_tag="ACHBFA_001305"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM9394809.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(17257..17595)
                     /locus_tag="ACHBFA_001306"
     CDS             complement(17257..17595)
                     /locus_tag="ACHBFA_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394810.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(18021..>18263)
                     /locus_tag="ACHBFA_001307"
     CDS             complement(18021..>18263)
                     /locus_tag="ACHBFA_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9394811.1"
                     /translation="CASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKII
                     RIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         5350 a         3942 c         2855 g         6116 t
ORIGIN      
        1 ttataacaat tgggttaaaa agaaaataga tagtattcta cctcatattt atttgaaagt
       61 aatatataaa agacgttcaa attcgaacgc ctctcgttaa ccaaatattg gattcagaat
      121 ctagttttca tatataaaca gagtatgatt aggtgcaaaa attgcacttt tattgcactt
      181 ttatttttat ttatagtgtt ttactgttct gtatattgtt ttaactccct tgatagtatc
      241 gcattctgtt ttatacactt tacgttgaaa acagagtggg aataaagagc caacataata
      301 tggaggggga atccctataa atcaacattc ttcaatagct aaaatgcttg attttcatta
      361 aaaataagat ggttccctac caaaagagac tataatataa gttaatttgt ttcattctta
      421 tcataaaaag aagttctcga aaaacgaact gttttccgag aacttttttc tatccatcta
      481 agtgctcaaa tcatattcac gacggaattc taaggttacc ctttcttaaa caaactttat
      541 gttatctgca caactttttg caattttttc attgcttctt acgaaatcgt tgaactctct
      601 ttagcataat acaaagtgta gatgaagtct ggtgctttac tcgttttggt aaaaatgact
      661 ggaaatcgtt tctggttatt tttggcaaag gcgaggtgca ggttcgattc ctgccttcta
      721 cgctaacgat gtggctgaag ggaacctccg atcttgaatc gaaacgatcc gcagtctatt
      781 gtttcaccaa tgaaacaaaa tcaacgttcg tccaagcatt tcctgaagcc ttgctataaa
      841 ccccaatttc acaaacacct gttgtaattg gaatgtcttt gattgtaatt tttgtccatt
      901 cacttgtttt aacaaccgca gtatttagtt ccgctccacc ataatttttg atatataact
      961 gattagtatc aaactctccc ccagacatta cataggcact caatgtgtaa gttcctttgc
     1021 ttatgccact aacattttga taagctgatg cctcgtatgc ttgatcctgc caaaaagtta
     1081 gtttgtagtc cccaaaaaaa gtgcccgctt ccgttttgat cgtaccctta gcggttcctt
     1141 gttgaaacca ggtttgccaa ttggaaggac tggtcgttgg tccatcgctt tcgaaacttg
     1201 catttttaac tagatttatt tgcgcccctg cttgtttaaa cgcacgcaag cttggcaagg
     1261 cgtttccagc aaaatcaaac atcgcttggt tgtcccattc gtttcccata gtggattgaa
     1321 cactgagata attcatacct gcttgtgttg cccaactcac atttccgttg taccaaagag
     1381 gttcccaata gaagaagccc gccccgcgat tattcggtac tgcctgaatc cgatcggcaa
     1441 gatccagcaa atactctgct tgtcctgtcg gtgttggttc atatcccccc gcagctgctt
     1501 cggcttcacc aaaagcattt ggcatagtat cggcattcgc tgttgtatgc ccataagctg
     1561 tttcaactac gatcacttct ttaccatacc gttggctgat cgcattcata ttggtttgaa
     1621 gttcattcat tgaaccgtgc caatacggat aatacgacac tccaattaaa tcaaatgaca
     1681 cacctcgact cgtgatttca tcaaaccacc aagtaaatgc tcctgtatcg ccaccatcag
     1741 ctaagtggag cattactgga gttgttttag gttgagtgtc tcgaacagct tggataccag
     1801 acttcaaaaa agcagccaat cgatcaaatt ctcccccgcc ttctccccaa gacttgccat
     1861 aaggccagag cattccgcta ttcagttcat tgccgatctg aaccatatca ggataaacgt
     1921 ccagctgctt catttccgac agaacgttct ttgtatagtc gtacaatgcc gtattcattt
     1981 cttcaaaggt cagaccttgc catgctttag gaagattctg tttcccagga tcaacccaaa
     2041 aatcactgta gtgaaaatcc agcagcacct taagtcccag attttttgct cgttttgcaa
     2101 ggtcaatcgt cgtttccaga tcatttgtcc ctgctccata actttctcca ttactattat
     2161 aaggatcctg ccacaaacgc agtcgaacat agttgacacc attatttttt aaaatctcta
     2221 aggcatcttt tgaaatatcg tcttcataat attttgcacc agatttttcc atatctgaca
     2281 ggatcgaaat atctgctccg cgaataaatt cgctggcaga tgcttcaaca gtttttgaaa
     2341 cagcccccaa agcccctaac acagctgcaa ataaaacagc tttacgcaac caaattccca
     2401 ttttcattat ggctcctcct ttattgaaat tggttcagaa taaactgaac agctttttca
     2461 ggatctctcg ttaatccgat atattctgct ccttttgtcg caaccagctt gattcctaag
     2521 cgatcggtgt cttctttgat atcatcatag taagaattga cttgcggtgc taagaccacc
     2581 atatcaaaat cctgcagcat ttcgtaatga gaaccataag cacccgccat tgcactgatc
     2641 ggtgctttcg attcagatgc accttttttt aaagcattag ctaacatcgc acttgtccct
     2701 gcgccagcac aaagtaccaa cacattgatt tctttatttg catcgctgat aacttgactc
     2761 gctgccacag gtacttcctc acgtacatcg gcagaagcac cttcctcggt tgcgatcacc
     2821 tctgcttttt gtgcttcttt ctctactaag acagcatcgt atgctttgca aaatggtaga
     2881 tagatcagga agtcaacgac gattagaacc gctgctagaa caaaagctaa gatacccatg
     2941 cccgttccta gaaccaaacc gatcggtgct ggagttgccc aaggcaaaac gtacataaag
     3001 ctgttcatgc ccaatacatc gacaaaaaac ttgaacaggc aaacgtttac aacaggtgtt
     3061 aacaaaaatg gaataaaaaa ataagggttc aaaatcatgg gtgccgcaaa tagcaatggt
     3121 tcgttgacag cgaaacttac tggaataaag gaagctttcc caactgcctt caactgctta
     3181 gacttagcaa agagtaggaa gataaaagga acaaccaatg ttgccccagt tcctcccata
     3241 gttcctacaa aattccctaa acccaccgtc agtactttag aagcttgttc accggaattg
     3301 aacaattgca gattggtttc cacattggca tagataattg ccgcaattgc cggctccacg
     3361 atcgacggtc catgaacacc gacaaaccaa aacagcgcca tcgctcccca gataatagcg
     3421 attcctaagt acccgtcagc tgctgtaaat agcggctgca gcaccgtaat gattgcttct
     3481 gcaaatgaga tttggaagat ttgtcgaatg cctaaatcaa ttaaaacggc cgcaaatact
     3541 gaaaaagaga aaggaaaaat atctcggaag gtttgcgaaa tcgttccagg aacttcttta
     3601 ggcattttaa tggtaatatc tttcagcaca caaaacttat ataaattaac tgttaaaaat
     3661 gcagcgacaa aggaagccag tagtcccttt gtccccatat attccgttga aaatccacct
     3721 tcaatcggag agacgcttaa caacaggaaa cttaccacag aggccagcat cacagaggtg
     3781 gtgttgatta ctttgttttt cggtaagcga cgattcatcg aatcagccaa gcagcgagca
     3841 gtggttgctg aaacgagtaa ccctacaaca cccatcgaat aattatagac tttccatagc
     3901 catgtggaga cagattcagg ccattggtaa ccaaaaatct caggaaccgc agcaatcaaa
     3961 atgaaaatgc ttgaaaataa aatagctggc atcgctgtta aaaatccgtc acgaacggct
     4021 cctaaataac tattacgtga gattttttca aaaaatggtt tccctttttc gatttgttta
     4081 atgattccat ccatagcgtt cttctcctat ccttgattct gtctgtacag tacaataaag
     4141 tcttgaatca gatctctcag caataaagtg gtcattaaat gatcctgccc atggataaaa
     4201 atgaatccca tctccatctc tttcccagat gcttcttccg ctaaaatttt tgtttgcgaa
     4261 ttatgtgcaa gggttaaatt ttcgtcggct tctttcaacg ccgaatcaac atgttcaaag
     4321 tttccttgtc ttacttcttt caataatttc aataatgtac tgcgagcgtc gcctgaatat
     4381 gcaacgatct caaaaccaag catttgtaat tcctctttat tcatcttttc ttcctccatt
     4441 atcacttatt aaataatcgt tttggtttca ctgaccaact tgtaccagta agctgattct
     4501 tttgggtaac gcttctgggt ttcaaaatca acataaaaca agccatacct tttgttatac
     4561 ccatttgtcc atgagaaaag atccataagc gaccacaaaa aatacccctt cacatttacc
     4621 ccggctgtta tcgccttgct taaggattcc aaatagactc tcaaatagtc gattctcggc
     4681 tgatccataa taatgccatc ttcaaactga tctttatacc ccatcccgtt ctcagtgata
     4741 tagattttgt tgtaatgtgg gtaatcgctt ttaattcgca gcaacaagtc atatagacct
     4801 tctggataaa tcagccagtc ccaatcagtt cgaggaattc cttctttata aatacgttct
     4861 ccgattccct tcactttata aacagaggtc cctttttcac ccgtaccgtt atgatggatt
     4921 gcattttctc catcgtaagc tttgacaaaa tggcattggt agtgattgat ccctaagtag
     4981 tcattacgcg tggatgcttt tttcaattca acgaaatctt cttcaggaaa atgatagctg
     5041 gcctgatttg cttcacatat ctcatcaaga gcagtcaagg tttcagttga ataatagcct
     5101 aaataggtag catctaataa gaaacggatc gacaatgcat cgtctaaaaa ggcagcatgc
     5161 ttatcttccg gcgcgtctgt tgccgcatat ttcgtctcta atgagtgaac tacaccaatt
     5221 tcacccggaa gttcgttttc tttgaaatag ttcactacac gggcatgagc aaccatcata
     5281 ttatgaagac aggcgacgat ctttgtaaaa tcatatttga ttcctggtgg gaaaacacct
     5341 aacaaatatt gattggttgc aactggatag atttcattaa aggtactcca caccttgact
     5401 tctttgaatt catgaaaaca aaaaatggca taggaaacaa atgcttctat tgtttctcga
     5461 ttcaaaaaat ctccatggtc aaacaaacgt ttaggggtat caaaatgatg caacgtcaca
     5521 aacggcgtta cgtttctttt cttacattct tcgaacacac gatgataaaa agcgattcct
     5581 tctggatttg gtttcccagc tccatcagga aaaatgcggc tccaagcaat ggataatctt
     5641 agaccattta caccaaaacg ttcacacaat tcaatgtcct ttgcatattg atgataaaaa
     5701 tcacttgccg gatcgggact gaatcgccct tgttcctcta aaaagtcatc ccaagcaaca
     5761 gcgcctttgc caccttcctt agtggctcct tcaacctgat aggctgctgt ggctccgcca
     5821 aaaataaaat cttctggtaa tttaagcatg tacatctctc ctcaaatcta taatcagttt
     5881 tcgctctttc taactgattg cattgtaaac gcatccaaaa catttgtcaa caaaaacaaa
     5941 cacaaataat cataaaaacc agaaaacagc aataaaatct attgtaacaa acataaaaac
     6001 caccctaaat gtttactttt gttcgaatta gattgactgt tgtccgttta tatgatagat
     6061 tatccttaaa ttagaggtga agacaatgtt gaaaaaagaa agattgctta ctatcgtaga
     6121 aatggtcaat aaaaaaggaa tccttaccgt taatgaaatc atcaatcaat tagatgtttc
     6181 tgatatgacc gttcgcagag atttagatga attagaaaaa tcgggcaagc tgctgcgtgt
     6241 ccatggaggg gctcaaagtc tttcctacac tttagatcaa gaactttctc acactgaaaa
     6301 agccactctg cagattcaag aaaaaaaaga aattgctgcc acagcagcct ccttgattca
     6361 cgagggagaa acgattttct taggaccagg tacaacaatc gaactattgg cgacgcattt
     6421 actgaacaag aaaattcgga tcattacaaa taattatcca gtttttgata ttcttgctca
     6481 agcagatgga ccagaaattc tactgattgg cggtgatttc cggaaaaata ccggcgcatt
     6541 tgtcggaccg atcaccaacg acaatttaag aagactaaag tttacaaaag catttatcag
     6601 ttcaaatggc gttcacaacg aagcgatctc tacttacagt accgaagaag gagaagcaca
     6661 gcagatcgct ctaaataatt cccgtaagaa atatctactc gttgataata aaaaattcaa
     6721 tcgagatgat ttttatgtgt tttataattt gcatgatttc gatctgctca ttacggatcg
     6781 ccagatctcg cgggaggttc aagagcacta tgcacaatat gtcgatctgc aaattgctga
     6841 ttcaaccgaa aataccacta taggagcgta actatgaaag ggattatatt tgatttcaac
     6901 ggaacaatgt ttttggactc tcatctgcat gaagctgctt ggctgcatat gatacatgat
     6961 catacaaaag atggtttatc agacgaggat attttaaaaa atattcatgg tcggacgaat
     7021 acggagattt tgaaacattt catttcagag catttgaccg cctctgagat cgccgtattg
     7081 tcagaagaaa aagaaagcta ttatcgtatg ctttgcttgc aaaatgaaaa cgagcttcag
     7141 ttgacaaaag ggcttgaagc gactcttgac cagctgactg accaacagat tccattaacg
     7201 attgccacgg ctactgtcaa agagaatgtt gctttttatt ttgatatttt cgatttagcc
     7261 cgctggtttg actgggacaa gattgtctat gacgacggct ctttccctgg gaaaccgcaa
     7321 ccggatattt ttcttaaagc ggcagacaaa ctgagcttag ctccggctga ttgtcttgtt
     7381 atcgaagatg cttactcagg attacttgct gccaaacgtg cggggatcgg aacaattatt
     7441 gcaattgatc cattcggtaa aaacaaaaca gtatttgaac aggcacagct aaataaagat
     7501 ggaaccatca aagattttac tggtttttgg acagagcacc tgtctgccac atccgttaat
     7561 tcttaaacga ttcaactaac taccaaagaa aagcaactga aatgcacacc tccttttata
     7621 aaaaactgcc agtgaaacga catgaaagat acactgctat atccttagtg aaatatagca
     7681 gtgtatcttt gactttttca ttcttttaac catatgacgc gttcatctct atctataatt
     7741 aacattaaaa atagtgtttt cacattattt ctcaaaccaa tcttaaaaat catcaggagt
     7801 acttttttga tatactttta ttaatgctgg gcgtaatggt taaaagattt ccactagttc
     7861 ctctccacca agctgagttg aaaaatgctt atctgtaaac aacatagaca aagaggaaat
     7921 aaacgtttcc gtatctttct ctgtcccaag gatattaatt aattttccta ctaggtaagt
     7981 atcattaaac ttaagatctg ccttcacatc aaaagttttg ataaaagctt ttatcatgcg
     8041 tacaattttg tctttatcgc cataattgat ttcaccactc atatcaatat agtcactaat
     8101 ccctgaagaa ataatggcaa ctgcttcttc tttcgcctta tcagataatt catttaatga
     8161 ctgggattga ttgatgactt cttgagtttt atctgttacg taatctctca ttatcctatc
     8221 ggcatactcc gcaaagtaat cttgaaaaaa ttcttctaca gggtaaccaa cttccgtctc
     8281 ccttagttaa agatatgaac cctatttatc agtctacttt tccaataaat cggtagtata
     8341 tctctatttc ctgtattctt tcgttctcct cattcgttgt tgcttcgtga atgagtattt
     8401 tttctatcat tgcatttaat aacggtgctg tcagttcctt tggtacggaa tactccttga
     8461 ttaactcaat ccatttttca gcacctatca tatcctgttc cattttactt aattcttctc
     8521 ttaagtcggt tatctgctgt tccagttcta tctgttcctt ttgatatttt cccgacagca
     8581 taatgaagtt tcgttctgtt atcttctcac aggctctatc ttcatacatt ttcgcaaata
     8641 aacagtcaat ctcattttgt cggttctcgg ctttcttcaa ggcacttgcc tttttcttct
     8701 tttcccgtat tcgctcggca ttaccgactt tctgtatctt atgcaatacc ttttcttcgt
     8761 cctgctgtac tgccttagcc caatactgca atcgctccaa tacagcttga tacagcacat
     8821 catagcggat atagtgcata gaacaatagc ctttgccaaa ttgtccatag aaactgcaag
     8881 cataataact gtaaggcgtc ttatttgcct tatttgttgc aaatctcata gaccagccac
     8941 aatccgcaca cttaacaagc cctgcaaata tcggcgttgc cttttccttt gtctgtctac
     9001 gccttgattt tatctgttcc tgaacacgat agaacacttc tttttcaata attggctcgt
     9061 gggtattttc tactcgaaac cattcgcttt cgggctttcg cactttcttc ttgcttttga
     9121 atgatactgt tgactgtcga ttatgaacgc tgttgccgat ataaacttca cttttcaaaa
     9181 ttcctttgat ttgtgcaatc gtccactcat aacgcttact ttcgggtttg ccctcaaaaa
     9241 tatgtgcaaa agtaccatac cttgtaaaat tcaaccaaga cgatgtcggg actttttctt
     9301 ctcgcagtat ttttgtgatt tttgcactac cgtaaccttg ataggcgaga gagaagattt
     9361 tttcaacaat ccattttgtt tcctcatcaa ccaacagttt ccctttgatg tcggggtgtt
     9421 tcttatatcc taacggagca taagccccat aatacgcacc ctctgcaaat tttgtcttaa
     9481 aggctgattt cactttacgg cttgtatctc ttgccaccca ttcattgata atattcttaa
     9541 atggtgcaat ctcactttcg cctttttcgc tgtctacacc gtcgtcaatc gctatgtaac
     9601 ggacattatg gctgggaaaa tacaattctg tatattgtcc tgtcataata tagtttctgc
     9661 caagtcggga aaggtctttt gttacaacac agttgatttt tcctacctct atatcctcaa
     9721 tcattctctg aaaactcggt ctttcaaaat ttgttcccga ccagccgtca tcaatatatt
     9781 catcaatgac attcaaatga tgttcttttg catatagacg caacatactt ctttgtgtgg
     9841 taatgctgga actttcgcct tgtaattcat catctcggct caacctaaga taaagtgcag
     9901 tattgtaaat ctgttgtttc attgtcagtt atcctccttt ataactaacc cgtgtttaca
     9961 atacctgctt gcaagtaaag tataacacgg gtactttttg ccattcaatc tatttcttac
    10021 aactgcaccg aatttatgcg attgtctgct ttgctttctc aagcataaca tcagttaaaa
    10081 gttcttccat tgtctttccc ttttcggaaa aatgctcttt tacaacaatc tttgtctttc
    10141 ctctgtttcc aatgccacac ttgttatttt tcttttgaat tgtttttaca gcattatcaa
    10201 taacacatac ccccctttct gtaaaatctt tctaagcaat tcaacagctt ttgtcatagc
    10261 ccacaggagt tccacctctg cattatccaa tgtagccgta cccattgcgt gcgacgcttt
    10321 taacgctcaa gctgtggctg tacgggagta tcattatcag acaggacagg tcatggcgat
    10381 aaaagaggac aagtctttta caagaacaca gataggttgt cgctcgcttt tgcaggggaa
    10441 aggtcatggc gtatctgttt caatggctta ctgtctgtct aatgtattga attgctttat
    10501 tcaattgtca aagaacacga aagaagaaaa tcgtctttcc tatataccgc gttggaaaag
    10561 agcaaaagcg taaccaatac tcaaaacttt tttctttttc tgtaccatat agggaaagaa
    10621 aatgtcattc tgctaagttg atgatgaaaa gatttctttt cacaccctaa ctacccatgc
    10681 aacaccgtta ctgccaacta tggaatgaaa gattttcttt gctccatata gggaatggaa
    10741 actgctgatt gttaagttga gaatgaaaaa aatttcttct tttcacataa cactgtccgc
    10801 ttaactcgat tttccgacat ttttcaaaac ttttcttctt tcacttcata tagggaatgg
    10861 aaacctcgat ttaatgacct attttcaaaa aagtttagat ttccttttct cgtaccatat
    10921 ctgtacgcta gttgatgttt tgttgcagat tttttaaaga aattaaaaaa taccgtcatt
    10981 ctcttgtgga acaacggtat ctatgcgttc ttctatggtt gatttttcta actgtaataa
    11041 atgtttatgc ttgtctttga gttctgcctg ttcaatcata tcttttagta tcgttgtatg
    11101 attttctttg tctaatgcct gtaccatttt tatggttggt gcaacctgtc tttgtagcca
    11161 atgcaatgct ttttgtaaag tataaggctc tggctttgtg gttaagcgta tcggctctct
    11221 gttttctcct acaaaccacg cccaatcatc attcattttc cattgacttt tcggtttatc
    11281 gtcctctcta tctacaaaac ggacataatg attgatgata gaaaaagcag tacgttcagc
    11341 gtcccgataa gtcagtaaat ctacaaccgc ataataggct cgttcattct tcatgcgtat
    11401 ttcaaaacgg ttcttgattt ctgtatcttc aacttctgtg ccattcttga catactgctc
    11461 ataattcttc tgataagcac acatatataa ttcgctactt gttgaaccga gatataaggt
    11521 ttcgcctgta ttttgtggtg tatcataccc atttttctct gtaccaccgt aatctttctg
    11581 tttgcgaaaa taggagatac attctccagc cttatatttt tctattagtt ttggaatatc
    11641 caagatacct gccttatcat tgattgccaa gtcaagccgt ttcagcttac cgcctgccgt
    11701 catacaacca agcataaagt cataccatga gcgttcctgt gccaagagat aactttccat
    11761 ttgccgacac cctctgcctt tcagttccaa caaaacacct aaatgctctt gcatggaaca
    11821 catgatgtta atgtcgccta aaacatattt actttcatat ccgtactttc caaaatcttc
    11881 ataaagcata taatcggctt ttagttgtaa tacatcacga ataatcttta atgcgtccgt
    11941 agtaggaaag cggacacgaa agtaatcaat tagtaaaaat aacggctctc gtggattgaa
    12001 acgctcaatc tgtttttcta tgatttcctt taattcgtta ttcaatggct gtttgccttt
    12061 ttcaatgcgg ttataatact cacgactgat accacaggca acagcaattc ttgtttgtgt
    12121 tactccgtat tcttcacgct tttgtcttaa ttcttctata aagttcttat cattcattct
    12181 tcaattcctc caatcaaaaa agacaactac catttactga taattgtcta tacttaaatt
    12241 tcttcaaaag aaaaagacaa tcaatttttc atttgaaatc aactgtcttt cctatgtctt
    12301 attcaatttt atgtgttaaa tgagtgtctt ttagttaaca ccctgttaaa agcggttttg
    12361 ccttgtaaat acagcgtttt ccgcttagta gtgtctattg tctcttgttt tttgtgcccc
    12421 ccttgttaga taacgggggc ttactgttcg ctcgcaagct cgctcaactg ccgaagtgaa
    12481 ccagccacta aaaacggcgg aaaaatctaa gagattttcc caccgtcgtg tctggatcac
    12541 tccgcctgtt atgttttttc gctttagctt ttaaccccaa gcatattata ctgttacaac
    12601 gcattttatt tgatgccata atttgcagaa ttcggaaaat taaaacatcc catttttagc
    12661 cttgttataa cggtaataat tttttctatt actgttataa caaggattcc aggggttcgg
    12721 ggatggaaga tccccgtatt atttctgcac tatttatttc tctgaaaatg gcatattata
    12781 atgtgctgat caacattata cctctaatgc acgaattaga tttaccattt caatagctcc
    12841 ctgtgcacat tcagagcctt tatttcctgc cttactgcca gctcgttcta tcgcctgttc
    12901 aatcgtatct gtcgtaagaa caccaaacat aactggtgtt tcagtattta aactgatttg
    12961 agcaattcct tttgatacct cactacacac ataatcataa tggcttgtac tacctcgaat
    13021 aactgctccc aagcagataa ttgcatcata ttttttactt tttaccattt tagacgctat
    13081 cataggtatt tcaaatgctc ccggaaccca agctacctct atgtcctttt catcaacatt
    13141 ttctcttttc aaattatcta atgcacctgc taatagctta gaagttataa attcattaaa
    13201 ccttgctaca acaataccaa tttttatatt ttctgctact aaatttcctt caaaagtgtt
    13261 catctatttt tcctccatat tcaaaatgtg tcccattcgg tctttctttg tctttaaata
    13321 aaaactatca taaggatttg cttttatttc aatcggtact ctgcttgaaa tttgcattcc
    13381 ataatcttct aactgataaa ctttatcagg attatttgta agtaaatgca atgattttat
    13441 tcccaaatct cttaaaatct gtgctccaat ataatattct cttaaatcgc cttgaaatcc
    13501 tagtgcaagg ttggcgtcaa gtgtgtccat acccttatcc tgtaaatgat aggcttttag
    13561 tttattgaca agcccgatac ctcgtccctc ttgtcgcata taaagcaata cgccagaacc
    13621 attttctgca atcattttca ttgctctatc taactgctgt ccacaatcac agcgtaaaga
    13681 gccaaagaca tctcctgtta aacactctga gtggacacga catagcacat catttccact
    13741 atttaactct cccataacca atgcaacatg atgttcgcca ttcaatttat tgatataaca
    13801 atgtgctttg aaatccccgt attttgtagg catttctaca actgaaacac attctacaag
    13861 catgtcatat acttttctgt attcctgtaa ttctttaatt gtgagggttg gtatatggtg
    13921 tatttgggaa aattgaatta aatcagatgt tctcatcatt ttcccgtttt cattcataat
    13981 ttcgcaacat aagccacatt cttttaatcc tgccaacctc aataagtcaa tcgttgcttc
    14041 cgtatgtccg tttctttcta aaacacctcc gttctttgca atcagaggaa acatgtgccc
    14101 cggtctacgg aaatcaaagg gatttatatc ctcaaccaca cacatacgag ctgtcaatcc
    14161 tctttcctcg gcagaaatac ctgttgtggt atccttataa tcaatagaaa ctgtaaaagc
    14221 agtttcatgg ttatctgtat tattttcaac cataggggaa agcataagcc tgttagccag
    14281 actttcactc ataggcatac agattaaacc ttttgcataa gtcgccataa aatttacatt
    14341 ttctgttgta gcaaattgtg ccgagcaaat taaatccccc tcattttctc tctctttatc
    14401 gtccataact aaaacaagtt ttccctcttg taacgcctgc aaagcctctt ttattttttt
    14461 atatttcata attcctttat cctttctgct aaaatccgta tcggcataac aactctttcg
    14521 acaactctgt ttcattgctg tttcctatga gtttcttcac atatttccct aatatatcat
    14581 tttcaaggtt taccatatcc ccgacttgtt tattcttgag aattgtttgt tccaaagtat
    14641 gagggataac ggatactgaa aaatctgctt tcgatacttt tgcaacagta agactaatac
    14701 catcaatagt aaccgaacct ttttcaacaa ttagatttaa gatttctctt tgcgtattga
    14761 tttgatacca caccgcattt ttgtctctac gcacagacga tattctgcct gtcccgtcaa
    14821 tatgccccgt aacaatgtga ccgcccaatc tgccattcat atgcatggct ctttctaaat
    14881 tcacaaggct tccatgtttt aacaggttaa gagccgttct gttccatgtt tcattcatca
    14941 catcaactat aaaagattgt cgtgtgaaac aagtaactgt aaggcagata ccattgactg
    15001 ctatgctgtc gcctaaatgt atatcctcta atatttttga agatttaatg gatagtttac
    15061 agttataagt atctttctgt attctttcta ctttaccgat ttcttctatt attcctgtaa
    15121 acatggataa atcacttcac tttctatgag ataatcgttt cccacttgag aaaaagcata
    15181 tggtttgagc ataatggcgt ctttaggaaa aacaacacct tgaccactta cagggctttt
    15241 tccactaccc ccaaatattt taggtgctat ataagtttgt acctcatcaa caatctgttg
    15301 ctcaagagca ctccaattca acgcacttcc accctctaaa agcagactgt ctatctgcat
    15361 atttcctaaa cattgcatta aattcattaa gtcaatatga ttaccttttt tcttcgtata
    15421 aataatttca caaccacatt ttctgtaaag agcaatttta ctttcatcaa tagaagaagt
    15481 agcgatatat gttttaatgt cattcgctgt ttggataatt tttgatgtaa tgggtgttct
    15541 taaatctgta tcacatacga tacgaatagg atttttcccg ttttctaatc ggcatgtcag
    15601 taaagggtta tcttgaataa cggtatttac tcctaccatg attgcagtaa ccttatgtct
    15661 gaattggtgc acttgttttc tcgctttttc gccacttatc cattttgatt gatttgtata
    15721 agttgctatt ttcccgtcca ttgtcattgc atatttcata aaaacatacg gaatacgctt
    15781 tgtaatatac ttcctaaata ctttgattaa ctgttgacat tctatttcta aaatgcctac
    15841 atcaacttga atgttatttt cctcaagttg ctttacacct tttccagaca caatcggatt
    15901 acagtctaaa gttccgacga ctacccttgt aataccactt ttaataattg tttctgtgca
    15961 aggtggtgtc ttaccatagt gacagcatgg ttcaagtgtt acataaagcg ttgctcctac
    16021 aggtgactct ctgcaatttt ttagtgcatt tctctctgca tgtaatccac caaaaacttc
    16081 atggtatccc tgtcctataa gccgattatt ttttacaatt actgctccaa ccataggatt
    16141 aggattgaca tagccagcac ccttttttgc cagttctatt gctattttca tgtattctat
    16201 atcgctcatt tttacacctc caaaaaaata catgccttga ataaaatcta tccaagacat
    16261 gcaaaagagg atttatgcga ttaaagaaaa tgctctgaaa caatataaaa tcattccaga
    16321 gcacgcaaaa aagcataaac ttacttttat cttctttcat ccagactata ctgtcggctt
    16381 tggaattaca ccaaatcatg cctttcggct cgtgggctgt accaccggta gggaattaca
    16441 ccctgccctg aagatagtta ttcaattacg gacaattata atatttgata ttatttctgt
    16501 caataaaaag tttttctctt gcttatccaa aatacaataa tttgcaagca ggtaaacaca
    16561 ggttatttct taatatcctt ttgtttggga aacttcgttt tcccaaccag gagaaatttc
    16621 attatcagta cttattaaat ctattaaaaa ccaacccgtt ctctgagtgt tgattctata
    16681 atatttttaa tttcctatat aaatcaaaaa tattatagaa tcaataagct aattaagaaa
    16741 caaactttag aaactctaga agaacttctg aatttatata gaagtgtgta gctacttact
    16801 aaatatacaa aagttaaaat aaaatactct ggatactctt tatactgcat taaagttagt
    16861 aatattaaca gcaaagatat cgaaaaaaca ataacataat atgtgtcaaa atttttcata
    16921 ataagaactc ccttactgtt gcatactaac ataatccaaa aactcttcta cttcagctaa
    16981 agacattcca ctttctttag tccaaaaaac agttttttct atcgatcctt ctctgtaaac
    17041 tgtaaatgtc ggagtcgagt taattgagta gcgactcttg aattctttcc actcattttc
    17101 atcttctcta acttttgcta cattcacgta ataaatattt tcttttccct tttctaccag
    17161 catatcgaat agcttcctgc ttttgatgat cgatttcttg tgctacatct agtaaatttt
    17221 catcaatttc tatatcataa agtatttcac tagtgtctac atccacaata tatacttttt
    17281 atcagtagca atttttgagt aatccgttaa aatgatctca gtctcgtcac tttcatcagt
    17341 ataaatatac gttaacaatg aactcacctt ttcaatcggt gttagctcta agcctactgt
    17401 attttttact attgagtcat tatttaccac cataacttcg tactgtatat accatcctat
    17461 ttggtttcca tcaatatcag taattggctc actagctaat ggagtctccg gaacaatatt
    17521 ttcctgatat ttcttaaact cttctgtagc acgaaaaagg ttatcatgtt cctcacttga
    17581 aactggttga gtcatagaaa atgttacttg gctaaacaga gggaaaataa acactacaat
    17641 caacaaagta taaaaaaaag cccttctttt tcatttttta tcttccaatc acaaattgaa
    17701 aaacttattc ctgcaacttt gagtctagta tcacaatcga ctgactacaa ctattcattt
    17761 acattctact ttaagttcct ccttaaccat taattttatt aaagatactt gtaatagtga
    17821 ttctcacaaa atttcatgaa aaatacttta ttactgtatc taaaattcca atacacaact
    17881 tatcgactat tatctgaaaa ccaaaactta ttattccttg atgttattct agcaatagta
    17941 actgcttttt tatttttttg catatttttt agtcaagtcc agactcctgt gtaaaatgct
    18001 atacaatgtt tttaccattt ctacttatca aaattgatgt attttcttga agaataaatc
    18061 cattcatcat gtaggtccat aagaacggct ccaattaagc gattggctga tgtttgattg
    18121 gggaagatgc gaataatctt ttctcttctg cgtacttctt gattcagtcg ttcaattaga
    18181 ttggtactct ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg
    18241 aatccatcat ccaatgatgc gca
//
LOCUS       DAYYNG010000048        18168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000048 DAYYNG010000000
VERSION     DAYYNG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.048"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..445)
                     /locus_tag="ACHBFA_001308"
     CDS             complement(161..445)
                     /locus_tag="ACHBFA_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394812.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="ACHBFA_001309"
     CDS             complement(463..1353)
                     /locus_tag="ACHBFA_001309"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM9394813.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="ACHBFA_001310"
     CDS             complement(1373..1591)
                     /locus_tag="ACHBFA_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9394814.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="ACHBFA_001311"
     CDS             complement(1561..3261)
                     /locus_tag="ACHBFA_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM9394815.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="ACHBFA_001312"
     CDS             3528..4301
                     /locus_tag="ACHBFA_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394816.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="ACHBFA_001313"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="ACHBFA_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM9394817.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="ACHBFA_001314"
     CDS             complement(5125..6096)
                     /locus_tag="ACHBFA_001314"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9394818.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="ACHBFA_001315"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="ACHBFA_001315"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9394819.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="ACHBFA_001316"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="ACHBFA_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9394820.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="ACHBFA_001317"
     CDS             complement(9051..9875)
                     /locus_tag="ACHBFA_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9394821.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="ACHBFA_001318"
     CDS             complement(9848..10528)
                     /locus_tag="ACHBFA_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9394822.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="ACHBFA_001319"
     CDS             complement(10551..11489)
                     /locus_tag="ACHBFA_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9394823.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /gene="ispE"
                     /locus_tag="ACHBFA_001320"
     CDS             complement(11734..12585)
                     /gene="ispE"
                     /locus_tag="ACHBFA_001320"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM9394824.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="ACHBFA_001321"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="ACHBFA_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9394825.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVVLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="ACHBFA_001322"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="ACHBFA_001322"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9394826.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="ACHBFA_001323"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="ACHBFA_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9394827.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         5242 a         3912 c         3112 g         5902 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcacca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttg
//
LOCUS       DAYYNG010000049        17882 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000049 DAYYNG010000000
VERSION     DAYYNG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.049"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..1011
                     /locus_tag="ACHBFA_001324"
     CDS             199..1011
                     /locus_tag="ACHBFA_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9394828.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            1008..1808
                     /locus_tag="ACHBFA_001325"
     CDS             1008..1808
                     /locus_tag="ACHBFA_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9394829.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1969..3237
                     /gene="rseP"
                     /locus_tag="ACHBFA_001326"
     CDS             1969..3237
                     /gene="rseP"
                     /locus_tag="ACHBFA_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9394830.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3305..5014
                     /locus_tag="ACHBFA_001327"
     CDS             3305..5014
                     /locus_tag="ACHBFA_001327"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9394831.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5226..9584
                     /locus_tag="ACHBFA_001328"
     CDS             5226..9584
                     /locus_tag="ACHBFA_001328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9394832.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9729..10202
                     /gene="rimP"
                     /locus_tag="ACHBFA_001329"
     CDS             9729..10202
                     /gene="rimP"
                     /locus_tag="ACHBFA_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9394833.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10225..11400
                     /gene="nusA"
                     /locus_tag="ACHBFA_001330"
     CDS             10225..11400
                     /gene="nusA"
                     /locus_tag="ACHBFA_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9394834.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11422..11715
                     /locus_tag="ACHBFA_001331"
     CDS             11422..11715
                     /locus_tag="ACHBFA_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9394835.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11712..12023
                     /locus_tag="ACHBFA_001332"
     CDS             11712..12023
                     /locus_tag="ACHBFA_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9394836.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            12036..14342
                     /gene="infB"
                     /locus_tag="ACHBFA_001333"
     CDS             12036..14342
                     /gene="infB"
                     /locus_tag="ACHBFA_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9394837.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14369..14716
                     /gene="rbfA"
                     /locus_tag="ACHBFA_001334"
     CDS             14369..14716
                     /gene="rbfA"
                     /locus_tag="ACHBFA_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9394838.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14873..15329)
                     /locus_tag="ACHBFA_001335"
                     /pseudo
     CDS             complement(14873..15329)
                     /locus_tag="ACHBFA_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15494..17856
                     /locus_tag="ACHBFA_001336"
                     /pseudo
     CDS             15494..17856
                     /locus_tag="ACHBFA_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
BASE COUNT         6108 a         3169 c         3919 g         4686 t
ORIGIN      
        1 ttaataaaat cataaaaaca gaggggcgga tcattccgct tcctctgttt ttatttatat
       61 ttcttttatt taaaaattaa aaaaagaaaa aattattcag tttttaaata aaaaaacaat
      121 gatttcatag gaaaaacagt tagatattgc tataatactt aagatagtgg gaatctttta
      181 agtataggag gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata tattcaagaa
      241 aaaagcgaat ttcaatttaa taccgatgga ccaattcctc agcacattgc catcatcatg
      301 gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg ccataaagaa
      361 ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt gaaagtgttg
      421 acgctttatg ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt aagttttctc
      481 atgcaactac ctgtcgactt ctttgatacg ttcgtaccag aattgattaa agaaaatgtc
      541 aaagttcatg tcatgggtta tgaaaacgta ttgccagaac atacgcaaga tgctgtaaga
      601 cgtgcaattg agcaaacaaa aaacaatact ggaatggtat tgaattttgc tttgaattat
      661 ggaagcagag cagagatcgt gactgctgtc caagaaattg ccgaagaagt agcaaaagga
      721 gaaatacacg cagaagaaat cgatgatgaa ttgattgcta agcatttaat gacaggtttc
      781 ttgcctaaag agttacaaga tcctgagctg atgatccgta caagtggaga agagcgaatt
      841 agtaattttc tgttatggca gatcgcatat agtgagctgt attttacaaa agcgctttgg
      901 cctgattttg atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa tcgagaccgt
      961 cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg aaacaacgtg
     1021 tggtcacagc agccgtggcg ctagtcttgt ttattccgat tatatggatc ggaggcatag
     1081 cggttgagct tacagcagca atcttagcag taataggagt ttacgagctg tttcggatga
     1141 aaggattgac actcttaagt tttgaaggag ttttatccgc cgttggtgca gtgttacttg
     1201 ttttgccaaa agaaagatgg tttttctttc ttccagagaa tgtaagtaca tttatgttat
     1261 tttatcttat agttatgatt cttttaggaa catcagttat ttctaaaaat acgtatacga
     1321 ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt gggaataggt ttccagaatt
     1381 ttgtcaatgc acgaacagaa agcttggctg ttttgatatt tggtctattt attgtttggg
     1441 caactgatat cggagcatat atggttggtc gacgttatgg tcagcataag ttaagtcctg
     1501 atatttcgcc caacaaaacg attgaaggtg cgctcggtgg tattggtagt gctgttgtag
     1561 tcgcatttct ttatttattg ctatacccag caaaagaact atttaatcat ggtatgtttg
     1621 tcatgcttct gatgacgatt attttttcag cagtcggaca atttggtgat ttggtcgaat
     1681 ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa tatcttacca ggccatggtg
     1741 gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat ttatttggca
     1801 ttatttagta agtatacctg cgtttctttg tgaatttcat aaaagacgca ggtttttatt
     1861 tttcattata ttgatactat tggtgtcttt ggtatactat ggtaaaatta tagtgtttta
     1921 tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat gaaaacgatt
     1981 ctgacattta tcatcgtttt tggtatatta gtgattgttc atgagtttgg tcatttcttc
     2041 tttgcgaaac gatcaggaat cctcgttcgc gaatttgcta tcggtatggg tccgaaaatt
     2101 tatgggcatc aagcaaaaga cgggacaaca tatacgcttc gcctattacc tattggcggt
     2161 tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa tggcaccagg aatgcctctt
     2221 tctcttttac taaattctga cggcatcgta gagaagataa atttaagtaa gaaaatccaa
     2281 ttgacaaatg ctatcccgat ggaattgagc cgttatgatt tagaagatga gctgactatc
     2341 actggttatg tgaatggaga cgaaacagaa gtagtaacct accctgttga tcacgatgcg
     2401 acgattattg aaaacgacgg aacagaaatc agaattgcgc caaaagacgt acagttccaa
     2461 tcggctaaat tgtggcagcg tatgctgacc aactttgctg gacccatgaa taactttatt
     2521 ttggcaatcc tgttattcat cattctggca tttatgcaag gtggtgttca agtgacgaac
     2581 actaatcgcg taggagagat catgcctaat ggagctgcgg cagaagcagg cttaaaagaa
     2641 aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt catggaatga tctgacaaca
     2701 gtcattacga aaaatccgga taaaacatta gactttaaga tcgaacgaga aggtcaggtg
     2761 caatctgtcg acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt tggccaactt
     2821 ggtataaaag ccccaatgaa tacaggtttt atggataaga tcattggagg gacgcgtcaa
     2881 gctttcagcg gttcattgga aattttcaaa gccttaggat cactatttac aggtttcagt
     2941 ttagataaat taggcggacc tgtcatgatg taccaattgt cttcagaagc agcaaatcaa
     3001 ggtgtaacaa ctgttattag tttgatggcg cttctttcaa tgaatctcgg aatcgtcaat
     3061 ctgcttccga ttcctgcctt agatggcggg aaattagtct tgaatatttt tgaagggatt
     3121 cgcgggaaac cattaagtca agaaaaagaa ggaatattga cattagctgg ctttggattt
     3181 ttaatgttgt tgatggtgct agttacatgg aacgatattc aacgattctt tttctagaaa
     3241 aagttgacaa atagggtata ataacaaacg ataactattt tgaggaaata aaggagtata
     3301 tagaatgaga caatcaaaaa tgttgattcc aacattacgc gaagttccaa atgatgcaga
     3361 agtattaagc catcaaatct tgctaagagc tggatatatc cgccaagtat cagcgggtat
     3421 ttattcatat ctgccattag caaatcgtgt attggaaaaa ctgaaacgca ttatgcggga
     3481 agaatttgaa aaaatcggag cggtagaaat gctgatgcct gccatcttgc cagcagaact
     3541 ttgggaagaa tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt tcaaagaccg
     3601 caatgatcga aaaatgatcc ttggaccaac tcacgaagaa acttttactg atttgatgcg
     3661 taatgaaatc aattcatata agaaattacc tttaaatctt tatcaaatcc aacctaaata
     3721 tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga agagagttta tcatgaaaga
     3781 tgcgtattct ttccatgcaa cggaagaaag tttggacgag acatataaag attacgaaaa
     3841 agcctacacc gaagtattca agcgttgtgg cttagaattt cgctcgatta tcggagacgg
     3901 tggcgcaatg ggcgggaaag actcaaaaga attcatggcg atctcagaaa tcggggagga
     3961 tacgatctgt tattcgactg aaagtgatta tgctgcaaac ttggagatgg ctacaagcta
     4021 ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag ctacacctaa
     4081 cgtaaaatca attgacgaag tagcagattt cttttctgtt gaacctcaaa aaatcatcaa
     4141 gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt ttggtacgcg gagaccatga
     4201 agtcaatgac gtaaaactga aaaacttttt atctgctgac tttttagaag aagcaacaga
     4261 agaagaagca caaaaatatc taggtgctga gtttggttct gtcggaccag tcggtgtttc
     4321 agatgacgta aaagtatatg cagaccgtta tgtccaagat ttagcgaatg ccattactgg
     4381 agcgaacgaa acaggattcc actataaaaa tgtgaatcca gaacgcgatt tcactgtact
     4441 ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct tcaccagaca acaatggtgt
     4501 tcttgccttt actcgaggaa tcgaaatcgg acatatcttc aaaatcggca ctcgttacag
     4561 tgaatctatg ggagcaactg tactagacga aaatggacga gaaaaatttg tgattatggg
     4621 ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac attcagatga
     4681 acaaggaatc aactggccaa aaggaattgc gccatttgat ttgcatatcg tccaaatgaa
     4741 tctaaaagac gaataccaaa caaacctgac gaatgaacta gaagaagcga tgacaaaagc
     4801 tggctatgaa gtactggttg atgacagaaa tgaacgcgca ggagtgaaat tcgcggattc
     4861 tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg atggaatcgt
     4921 agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag aattggtgaa
     4981 tactttacca atcctcttga accaaggcga ataataagcg ttaaaacaca gaaaataaaa
     5041 atagctgaat cagctgtttt tgacaatctg atgattcgct tttaaaattg gaactgcgat
     5101 ttgaatcgca gttccaattt tttaatgtaa aactcagaaa aaacgagtat actaaaaggt
     5161 actgaaaaag aagaggataa gatgcgatca attgcattgt ttctcatcag aaagggcgta
     5221 tatctttgtc agaaaaacga gatttatttg aaaaactgtt ggaacaaatc caactagagg
     5281 aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat ggatagtgta gtggtccatc
     5341 gtaaatcgcg tttatgggaa tttacgctgc atttttccaa aattttgcct attatgctat
     5401 atcggtcatt gactcagcat ctgactatcg cttttaaaga tattgcacaa gtcaaactga
     5461 atatcttagc agatgaccag acattcgatg aacagctctt gcaagattac tgggcccaag
     5521 ctttggaaaa tcagcaatgc gatacaccac tagcacagca agtattgaaa acgcaggtac
     5581 ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag tacaggagct atttcttatt
     5641 tgaagcaaca atacttgcca ttagtagaag agttgttcgt cagttacgga ttcccaaaat
     5701 ttcgtatcga accagaagtg gatgagcagc aagcggaacg agtattgaaa ctttttgaag
     5761 aacgcaaaca agaacaggca gaagccttta tgaagcaagc agctgaaagt ctagttgtcc
     5821 acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga cccatccaca
     5881 tccagctagg acgaaatatt ccagccgatg aaccaacgac accaatgatc agtatcgttg
     5941 aagaagaacg aagagtcaca cttgaggggt acgtattcga caaagaagtc agagaactac
     6001 gttcaaaacg caagatcttg actttgaaaa tcacagatta tacttcatca tttatcgtca
     6061 aaaaattctc gaacggcgaa aaagatgaac aagtatttga tgcgatcagt gtcggtagct
     6121 ggttaaaggt acgaggaagt attcaagaag atacgtttgt ccgtgatttg gtcatgaatg
     6181 cgcaagatat catagaagtc aaacatacac ctcgcaaaga ttatgctcct gaaggggaaa
     6241 aacgagtaga gcttcatgtt catagcaata tgagtacgat ggatgcaaca aacagcatct
     6301 ctgatcttgt ggcacaagca gggaaatggg gacaccgagc tatcgccatt acagaccacg
     6361 gtggtgcgca agcctttccg gaagcacata gtgctggaaa aaaagcaggt gtgaaaatct
     6421 tatacggcgt ggaagcgaat gtcgtagatg atggtgtccc aattgcttat aatgatgcac
     6481 atgaagcatt gagtgaagcc acatatgtcg tatttgacgt ggaaacgaca ggattgtctg
     6541 cagtatatga cacgatcatc gagctagccg cagtcaaaat gtataaagga aatgtgatcg
     6601 aaagctttga tgaatttatc gatccaggac atcctttgtc acgaacaacg gtagatctga
     6661 ctggtatcac ggatggaatg gttcgaggat caaagtcgga agaagaagta ttgcggatgt
     6721 tccttgaatt ttcaaaagat acaattttgg tagcccacaa cgcggctttt gatatggggt
     6781 tcttgaatac tagctacgca aggtatggta ttcctgaagc ggccaatcca gtcattgata
     6841 cgttggaatt agctagatat ctgtatcctc aattcaaacg tttcggatta ggtgtgttat
     6901 ctaaaaaatt tggtgtcagc ttggaacagc atcaccgagc aatctatgat gctgaagcaa
     6961 ctggtcactt agcatggatc tttgtgaaag aggcaatgga caatcataac atgctttatc
     7021 atgatcagct aaatgagcat atcggagaag gagattctta caaaagggct cgacctttcc
     7081 atgtaacgat tttagcaaaa aatcaggcag gactgaaaga tttgttcaaa ctgatttcaa
     7141 tgtcgaatgt cgaatacttc gaacgcgtcc cacgaatacc acgctcccaa ttgaaaaaga
     7201 tgcgtgaaaa tctattgatc ggctcagctt gtgataaggg agaaatattc gaagccatga
     7261 tgcaaaaagg ggtagaagag gcaagaaacc gagcaaaatt ctacgattat attgaagtta
     7321 tgccaaaagc ggtctatgcg cctttgatcg agcaagaatt ggtaaaaaat gaacatgatt
     7381 tggaagaaat tatccaaaat ttggtagaaa tcggaaaaag tttggataaa atcgttgttg
     7441 caacaggtaa tgtacattat ctgaacgaag aagatgccat ttaccgaaaa attttgatca
     7501 attctatggg cggagcgaat ccgttgaatc gtcatagttt gccggatgtc catttccgaa
     7561 caaccgatga gatgctaact gcatttcact ttttagggga agaaacagcg aaagagatcg
     7621 tagtagaaaa tacgaataaa atcgcagata tctgcgaaga agtgattcca gtcaaagatg
     7681 aactttatac accgaagatt ccaggttcag aagatgaaat atcagaattg agttacacaa
     7741 aagcaaaaca aatgtacgga gatccgttac ctgagatcat tcaaaaacga ttaaaaaaag
     7801 agttgaattc tattaatggt aatggcttct cggttattta tctgatcgcc caaaaactag
     7861 tgcataaaag taatgaagat ggctatctag taggttcacg tggctctgtt ggctctagct
     7921 ttgttgcgac gatgacagga atcactgaag ttaatccgtt agcccctcat tattattgtc
     7981 cagagtgcca atattccgag ttttttgaag atggtacgta tggttctgga tttgacatgc
     8041 cagaaaaaca atgtccaaaa tgtggtgcac gcttaaacaa agacggtcat gatattccat
     8101 ttgagacatt tttaggattc catggagaca aagtgcccga tattgatttg aacttctcag
     8161 gtgattatca agcagaagcc cataactata cgaaagtatt gtttggcgaa gactatgttt
     8221 accgtgccgg aacgattggt acagtcgccg acaaaacagc atatggctat gtaaaaggat
     8281 acgaaaggga caataatctg cagtttcgaa gtgcagaagt cgacagatta gcaaaaggcg
     8341 ctactggagt caaacggaca accggccagc atccaggggg gatcatcgtt attcccgatt
     8401 atatggatgt ctatgatttt acgccgatcc aatatccagc agacgatcaa aattcggaat
     8461 ggaaaacgac tcattttgat tttcactcga ttcacgataa tgtattgaaa ttagatattc
     8521 tagggcatga tgacccaact gttattcgga tgcttcaaga tttatcaggg atcgatccac
     8581 aaacgatccc tacggatgac ccggaagtga tgcggatatt tgccggtcct gaagtacttg
     8641 gagtaagtca ggaacaaatt tattccaaaa cagggacatt aggtattcca gaattcggca
     8701 ctcgttttgt tcgagggatg ctagaagaaa cgcatcctac aacttttgca gaactactgc
     8761 aaatttccgg actatctcat ggtacagacg tatggttagg aaatgcggaa gaattgattc
     8821 gacgaggaga tgcgacactg gcggaagtaa tcggctgtcg tgatgatatt atggtttatt
     8881 tgatccatgc tggattagat agcgggatgg catttaaaat catggaaact gttcgtaaag
     8941 gacaatggaa caagatccct gatgaattgc gagaaaccta tctatcggcg atgaaagaaa
     9001 ataatgtgcc cgactggtat atcgattctt gttcaaaaat caaatatatg tttccgaaag
     9061 cccatgctgc agcttacgta ttgatggctc ttcgtgtcgc ttatttcaag gtttatttcc
     9121 ctattttgta ttactgtgca tacttttctg tacgtgcaga cgactttgat ctagtctcca
     9181 tgtgcaaagg aaaagatgct gtgaaacaag caatgaaaga aatcacggac aaaggattag
     9241 atgcttccgt aaaagaaaag aatcaactga cagtgctgga gttagccaat gaaatgctgg
     9301 aacgaggatt caaatttgga atgattgatt tgtataaatc ggatgccgtt aactttgtta
     9361 ttgaaggtga tacgttgatc gcaccattta gagctgtccc aagtttggga accaacgtgg
     9421 caaaacaaat cgttgaagca agaaaagacg gtccattttt atccaaagaa gatcttgcca
     9481 cccgcggtaa agtttcaaaa acattgatcg aatatatgaa cgacaatggc gtattgaaag
     9541 atctaccaga tgaaaatcaa ttatcgttat tcgatatgct ataagcatct gttttcctgt
     9601 ctgcttgtaa ttttaagcag agtatggtat agttcttata ggtaatgata ctaactgtga
     9661 gtgagcggat ttttcgctca ctctttttaa tggaattacg cacttaattt tttaaggagg
     9721 cgaaactttt gagtagcgtc gttgaaacag tcacagaaat ggtgacaccg attttagatg
     9781 aacaaaattt tgaacttgtc gaagtcgaat ttgtaaaaga aggaaaaagc tggttcttac
     9841 gagtatttat tgacaaagaa ggcggtatcg atatcgaaga atgcgccttt gtcagtgaga
     9901 aactgagtga aaaacttgat gcagcagatc cagatccgat tcctcaagct tatttcctgg
     9961 aggtatcctc cccgggagca gagcggccat tgaaaaaaga aagcgactat gaaaaggcac
    10021 ttggtgatta tgtccatgtc tccttatacc aaccagtaga tggcgaaaaa caatatgaag
    10081 gatttcttca atcatttgat gctgaacagc ttacattgaa aatacgcata aaaacacgag
    10141 aaaaagaatt gacttttgac cgaaaaaata ttgcaaaagc tcgcttagct atccaatttt
    10201 aatcacggag aggaatcaga aaaaatgagt aaagaaatgt taaatgcgtt agacgcttta
    10261 gaagctgaaa aagggatctc aaaagaaatc gtgatcgatg ctttagaagc agcattggtt
    10321 tctgcataca aacgtcacta tggccaagca caaaacgtcg aagtggaatt tgatcagaaa
    10381 aaaggaaaga tccatgtcta tgcggtaaaa gaagtaacgg aagaagtgat ggattcacag
    10441 ttggaagtct cattgaaaga tgcactttta atcaatccag catatgagat cggtgatacg
    10501 atccgttttg aagtaacacc aaaagatttt gggcggatcg ctgcgcaaac ggctaaacaa
    10561 gttattttac agcgtgtacg tgaagcagaa agaacgatta tttataacga attcagtgct
    10621 tatgaaaaag acatcatgca aggaatcgtc gaacgccagg ataaacgtta tatttatgtc
    10681 aatcttggta aaattgaggc agttctttct aaacaagatc aaatgccaaa tgaattttat
    10741 caaccacatg atcgaatcaa agtttatgtt tcccgagtag aaaatacgtc taaaggtccg
    10801 caagtatttg ttagccgcag ccatccagac ctattgcgcc gcttatttga gcaagaagta
    10861 ccagaagtct atgatggaat cgttgaaatt gtcagcatcg cccgtgaagc tggcgatcgt
    10921 tcaaaagtgg cagttcgctc aaatgatcca aatatcgatg cagtcggtac atgtgtcggg
    10981 ccaaagggac aacgagttca agcaatcgtc aatgaattaa aaggcgaaaa catggatatc
    11041 gtcgaatgga atgaagatcc agctgtattc attgcaaatg cattgaatcc tgcacaagtc
    11101 atggatgtga tcttcgatga gacaaatcca aaggcctgca cagttgtcgt tccagactac
    11161 caattatcgc tagcaatcgg taaacgcgga caaaacgcac gtttagctgc taaattgact
    11221 aaccataaaa tcgatatcaa atcagaatct gacatgacgg aattttatga aaatcaagaa
    11281 caacaaaaag aaacagaaga actgcatgat gaagcaatcg ttcagtcaga tttaacagac
    11341 gatgaatatg aaacaattgc attcaacaat gaaacggtcg aagaaaaacc agaagcttaa
    11401 ctttagtaag gaggcaaaga gatgaaacaa aggaaaattc ctttacgcaa atccgtggtt
    11461 tcaggtgaaa tgaaacctaa aaaagagatg gtccgaatca cccgttcaaa agaaggggaa
    11521 gtatcgatcg atccaactgg aaaaatgcct ggacgaggag cgtatgtctc acttgagccg
    11581 gaagaagtac aacaagcttg ggataaaaaa attctcgatc gagtactcga aacaacatta
    11641 acagatgagt tttatcaaga acttcttgac tatgtcactc accaaaaagc acgtaaagag
    11701 ttatttggaa aatgaataca gttaatcggc aaaaagccat gaatctcatc ggtctagcga
    11761 tgcgcgcggg aaaactaatc acgggtgaag aactgaccat tgctgaaatc cgcaaacaaa
    11821 aagcaaaatt ggtgtttgtg gcaaccgatg caagcgagaa tacaagaaaa aagataaaag
    11881 ataagagttc atattatgaa gttccttgct ttgagctatt ttctgaagca gaaatcacgc
    11941 agatgatcgg aaaacctcgc aaggtattcg gaataatgga tgccggattt gcgaagaaga
    12001 ccaaggagct aattgaaggt taggaaggtg attgcatggg caacaaaaga atctatgaat
    12061 tagcaaaaga actcaaaaaa tctagtaaag acgtcgtgga aaaagcacaa caacttggca
    12121 tcgatgtgaa aaaccatatg ggtgcgatta ctagcaatga tgagaagaaa ttgcaacaag
    12181 cgttcaaaaa cccaagcacg aaacaacaag ctgcacaaca agcaagcaga aaaccagcca
    12241 atcaacagcc gaacgatcaa cgtaaaaaag aaacaacgaa caatcaacaa aaaaataaaa
    12301 ataaccgcag ttatcaagat cgcggtcaag ggagcggtca agtgaatcaa ggaaaaaaac
    12361 caacaacaaa ccaaaataat acgggaaata ccagcagcca aaatcgtcaa ggaacgagcc
    12421 aaggcggcaa tcaaaaccgt actaacaata acaacaaacc aaacaataac aatcgcggca
    12481 aatttaataa taataaccgt aatcgttata ataaaaaagg taaaaaaggc aaacagcaac
    12541 aatcaactaa accagctgta ccaccacgca aattccgtga attgccagat gtattagagt
    12601 atactgaagg tatgaacgta gcagatattg cgaaaaaaat tcatcgtgag ccagcagaaa
    12661 tcattaaaaa attatttatg cttggtgtca tggtcaatca aaaccaagca ctggataaag
    12721 atacgattga actgttagcg acggactatg gcatggaacc acaagaaaaa gtccaagttg
    12781 acattgctga tatcgataaa ttcttcgagg cagaagaagt cgatccagat aaattagtct
    12841 cacgtccgcc agtcgtaacg attatgggac acgtagacca tgggaaaaca actttattgg
    12901 atacattacg ccattcacgt gtaacaagcg gcgaagccgg cggaatcaca cagcatatcg
    12961 gtgcctacca gatcgatatt gatggtaaac caatcacttt cttggataca ccaggacatg
    13021 cggcctttac aagtatgcgt gcacgtggag caagtatcac agatatcacg atcttagttg
    13081 ttgctgcaga tgatggcgtc atgccacaaa cagttgaagc aatcaaccat gccaaagctg
    13141 caggcgttcc aatcatcgtc gctgtaaata aaattgataa accaggtgca aaccctcaac
    13201 atgttatgca agaattaagt gagtatgagt taattcctga agcatggggt ggagaaacga
    13261 tctttgttga gatctcagcg aaattcggcc aaaacatcga agaattgctg gaaatgatcc
    13321 tgttagtcgc agaagtagaa gatttaaaag cagatccaac acaacgcgca atcggaacag
    13381 tgatcgaagc acgtcttgat aaaggtaaag gtcctgtgtc aaccttactt gtccaagaag
    13441 gaacacttca tgttggtgac ccaatcgttg tcggtaatac ttacggacgt gtccgtgtaa
    13501 tgacaaacga tcttggccgt cgcgataaag aagcaggacc tgcaacgcca gtagaaatca
    13561 ctggcttgaa tgatgtacct caagcaggtg accgtttcgt agtcttcgaa gacgaaaaga
    13621 cagcacgtgc agcgggtgaa gaacgcgcga aacgtgcatt attagaaaac cgtgctgcca
    13681 caagccgtgt gactttggat aacttatttg aaagcctgaa agaaggcgaa ttgaaagaag
    13741 ttaacgtaat catcaaagca gatgtacaag gttcagcaga agcactggct gcatcattga
    13801 agaagattga tgtggaaggt gtccgtgtaa aaatcgtcca ttcagctgtc ggagcaatca
    13861 atgaaagtga tgtaacctta gcagcagcca gcaacgctat tatcatcggt ttcaacgttc
    13921 gtcctacacc gcaggcaaaa gtgcaagctg atactgaaga agtcgatatt cgtcttcacc
    13981 gtatcatcta taaagccatc gaagaaatcg aaactgcgat gaaagggatg ctagaccctg
    14041 aatttgaaga aaaaatcacc ggtcaaatga tcgttcgtga aaccttcaaa gtttctaaag
    14101 ttggtacgat tgcaggtgcc tatgtaacag aaggatatat tcgtagagat agcggtgtcc
    14161 gtgtaatccg tgacggaatc gtaatctatg aaggccaact tgcaagtctg aaacgattca
    14221 aagatgacgt gaaagaagtc aaaatgggtt atgaatgtgg tgcaatgatt gaaaaattca
    14281 atgacatcaa agtagatgac gtaattgaag gatttatcat ggaagaagtc aaaaatgact
    14341 aaaaactgat aaaaggaggc caatcattat ggctaactac cgtgaccgcc gagtcggtca
    14401 agaaatctta aaagaagtca atgatatttt gcgcaaaaag gtccgtgacc ctcgtgtgga
    14461 gaacgtaacg atcacagatg tgaaagttac gggggactta cagcaagcaa cgatctatta
    14521 cagcatttta tctgacttag catcagataa gaaaacagca caagatggat tgaataaagc
    14581 atcaggattg attcgtcgag agttagggca caatctaagt atttataaga cacctgaatt
    14641 gttctttgaa cttgatgaat cagttgtcta tggcaaccat atcgatgaat tgcttcgcga
    14701 cttgaacaaa gactaatcaa aaactcccgc aaatcctgtg aaatcagggg ttgcgggatt
    14761 tttttgtttt cattaagacg tattacctaa gagttccatg actttagtct tgcggcgtgt
    14821 cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg actttctttt ttctagtagt
    14881 atataaattt ttgcatatag ataaagatca agataccgat aacggaagct agattgtaat
    14941 ctattttagt gacagtcagg tatagccaag taactagaag atcagtggaa cctgcaccat
    15001 aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa aatcacgttg aagttattga
    15061 tatttccgat tacgccgctt cgatttggtc acgatccaaa ttttggataa tccctgtaga
    15121 aattagcata gtagctggga taccaattca catattgaca aagataacag agacttttac
    15181 ataaaacgga tcaatcagga aaggaattgc tcgatccagc cccagttttc cagcaatgtg
    15241 ttaactggcc cagcgtcatt cagcaaatta tgcatgatga ggagcgaaat gaactgcgga
    15301 acagccattg tgataacgac aagttttttg gatcaatgta aaaaacgtca gcgaaaggaa
    15361 aaatttcgtg gttttttata cctgttcatt tggctagata taaaatccaa acaaatgaac
    15421 agaagtgctt attaggtttc aattgggagt atctgcgcta gaataagagt aataatgaaa
    15481 aggagtgtta tgtatggatt attctagtaa agaatacttt gataaaatga cggcttggtg
    15541 gcgtgctgct aattatcttt ctgttgggca actttatctt aaagataacc ccttactgag
    15601 acgtacactg aaacccgaag atgtcaaaaa acatccaatc ggtcattggg gaacgattcc
    15661 tggccaaaat tttatttatg tccatttgaa ccgagtcatc aataaatatg atttgaacat
    15721 gttttatatt gaagggccag gacacggcgg acaagtcatg gtatcgaatg cttatttaga
    15781 tggaagttac acagagatct atccagaagt aacggaagat gagacaggaa tgcaaaaatt
    15841 attcaaacga ttttcatttc ctggaggcat tgcttctcat gcagcaccag aaacaccagg
    15901 ttctatccat gaaggcggag agcttggata ctctctttca catgcagtcg gagctgtatt
    15961 agacaatcct gaagtaatct ctgctgtcgt gattggtgat ggtgaagcag aaaccggtcc
    16021 tttagctgga agctggttct caaatgtctt tatcaatcca gtgatagatg gagctgtttt
    16081 accgatcctt catttaaatg gtgcaaaaat agccaaccca acgatcttgg cacgtaaatc
    16141 agatggagaa ttagcaaatt actttaacgg acttggctgg gaacctttct ttattgaagg
    16201 aaatgattca gaaaaattaa atcctgttat ggctgaaaaa atggatcaag caatcgagaa
    16261 aattaaatcc atccaaaaag aagctcgcct gaagacagca acagatgtcg tgatgccaaa
    16321 atggccagta ttgatcgtac gcacaccaaa aggatggacc ggtccaaaag aatgggacgg
    16381 ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg attcctgtag atcaagaaca
    16441 tatggatcat gcagatgctt tactccgttg gctgaaatcc tatgaaccag aaaaattatt
    16501 tgatgcacaa ggacgtattt tagaagaaat cagagaaatc gcgcctactg gtgaccaaag
    16561 aatggcaaaa aatcctatca ccaatggtgg aatcgatcca agaccgctta ttatgccaga
    16621 ttggaaaaaa tatactttgc aatttgaaaa accaggctcc gttaaagcag aagatatgac
    16681 cgaacttggt aaattcgttc gcgagatcat tgagaaaaat ccagaaaatt tccgtatatt
    16741 tggaccagat gaaacaaaat caaaccgatt gaatcaagtt ttcaaaacga caaatcgcca
    16801 atggatggaa aaaatcgaac cagaaaatga tgaatggctt tcgccatctg gtcgcgtcat
    16861 cgacagtcaa ttatcggaac accaagatga agggttctta gaagggtacg ttctgactgg
    16921 acggcatggt tttttgcaag ttacgaatca ttcttgcgag tggttgactc tatgttgact
    16981 cagcacttca aatggatgcg gaaatcacat gacctttctt ggcgaaatga ttatccttcg
    17041 ctaaatctca ttgcaagctc aacagtcttc cagcaagatc acaatggata tagccaccaa
    17101 gatccaggaa tattgacaca tttggcagaa aagaaggcgg agttcatccg tgaatatctg
    17161 cctgcggatg caaatacatt gctcgcagtc atggataaag cattccgctc gtcagaaaaa
    17221 atcaatttga tcatctcttc caaacatccg cgtgctcaat tttactcagc agaagaagca
    17281 gccgtcttag tgaatgaagg cttgaaaatc attgattggg cttcgacagc aaaagaggag
    17341 gaacctgaat tggtcattgt tgctgcagga acggaatcta acttagaagc attagcagct
    17401 gtaactttgc tgctggaaga attcccaaaa ctaaaaattc ggttcatcaa tgtcgtagat
    17461 ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt tgagcgatga agaatttgat
    17521 caatatttta caaaagacaa accgatctta tttgctttcc acggttatga aacattggtc
    17581 cgcacgattt tctttgatcg tcataaccat catttaatga ttcatggcta caaagaaaat
    17641 ggagatatca cgacaccatt tgacatgcgt gtagtaaatg aacttgatcg ttatcatttg
    17701 gcaaaagacg cagctttaaa aatcaaagga agtcaagcag aagattttgc gaaaaaaatg
    17761 gatcagaaat tacaagaaca tcaaaactat attcgtgaaa atggtattga tctgccagaa
    17821 gtattggatt ggaaatggaa aaatcttgac caataaaatg ataaaatcga tcccgcaaaa
    17881 ta
//
LOCUS       DAYYNG010000050        17392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000050 DAYYNG010000000
VERSION     DAYYNG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.050"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..531
                     /locus_tag="ACHBFA_001337"
     CDS             163..531
                     /locus_tag="ACHBFA_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM9394839.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            712..1653
                     /locus_tag="ACHBFA_001338"
     CDS             712..1653
                     /locus_tag="ACHBFA_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9394840.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMEAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALSK"
     gene            1722..2453
                     /locus_tag="ACHBFA_001339"
     CDS             1722..2453
                     /locus_tag="ACHBFA_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9394841.1"
                     /translation="MKSLLYAIGDVHGKYELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLLHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2597..4777
                     /locus_tag="ACHBFA_001340"
     CDS             2597..4777
                     /locus_tag="ACHBFA_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9394842.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKKVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFLQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLTDILAGLT
                     QPGRDMRVEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4797..5279
                     /locus_tag="ACHBFA_001341"
     CDS             4797..5279
                     /locus_tag="ACHBFA_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9394843.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5295..6056
                     /locus_tag="ACHBFA_001342"
     CDS             5295..6056
                     /locus_tag="ACHBFA_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9394844.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVLEKILLMNDPTLQRRTQATFEKVW
                     KSEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6187..7521
                     /gene="murC"
                     /locus_tag="ACHBFA_001343"
     CDS             6187..7521
                     /gene="murC"
                     /locus_tag="ACHBFA_001343"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9394845.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     DVPIYYYGVTENDDIQARKIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMMTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7827..8312
                     /locus_tag="ACHBFA_001344"
     CDS             7827..8312
                     /locus_tag="ACHBFA_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM9394846.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8305..8988
                     /locus_tag="ACHBFA_001345"
     CDS             8305..8988
                     /locus_tag="ACHBFA_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM9394847.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     AVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9114..11759
                     /gene="polA"
                     /locus_tag="ACHBFA_001346"
     CDS             9114..11759
                     /gene="polA"
                     /locus_tag="ACHBFA_001346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9394848.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11804..12640
                     /gene="mutM"
                     /locus_tag="ACHBFA_001347"
     CDS             11804..12640
                     /gene="mutM"
                     /locus_tag="ACHBFA_001347"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9394849.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12604..13233
                     /gene="coaE"
                     /locus_tag="ACHBFA_001348"
     CDS             12604..13233
                     /gene="coaE"
                     /locus_tag="ACHBFA_001348"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9394850.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13285..13617
                     /locus_tag="ACHBFA_001349"
     CDS             13285..13617
                     /locus_tag="ACHBFA_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9394851.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13929..14420
                     /gene="nrdR"
                     /locus_tag="ACHBFA_001350"
     CDS             13929..14420
                     /gene="nrdR"
                     /locus_tag="ACHBFA_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9394852.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14443..15816
                     /locus_tag="ACHBFA_001351"
     CDS             14443..15816
                     /locus_tag="ACHBFA_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9394853.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15816..16742
                     /gene="dnaI"
                     /locus_tag="ACHBFA_001352"
     CDS             15816..16742
                     /gene="dnaI"
                     /locus_tag="ACHBFA_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9394854.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16871..17311
                     /locus_tag="ACHBFA_001353"
     CDS             16871..17311
                     /locus_tag="ACHBFA_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM9394855.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5937 a         3027 c         3833 g         4595 t
ORIGIN      
        1 aagtgaaagc gtaaataaca aaaacaacca acttatttat tattaaagaa aagcgtattt
       61 aagtaggacg attctgttga gagctttcgt agattttccc attttcctat ggtaaactga
      121 caaagttgag aatgttagaa cagatcgttt aggaggaaac agatgtttac gaatgaaata
      181 aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa cgttttggag agcagttggc
      241 tttgcagaaa aagatcgtca agaaatggat gggacgctta taattgaagt aggagttgaa
      301 gatagtcaaa cctcatttct gttatatgat cgagcatttg ttgagtccca ttcacctggc
      361 acagctacta gtgccccatc tttgatgttt tacagtaaag atatctttgg actatataaa
      421 aaaatgcaga cgcaatcagt cacattaggt gatatggtac agcttggaga agaatatgtc
      481 tttaactttg ctgatcccga tggcaactac tttgcggtta caggtaaata atagaaataa
      541 agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt tttaattcaa ataataatat
      601 tatgtaaata aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc
      661 gaacgccttc tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat
      721 caagtactgc tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat
      781 catttagcct tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga
      841 ataaatggta ctgtgtctgg aaccgttgaa aacacaactg cctatatgga agcaatgaaa
      901 gctgatcctc gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt
      961 aagaagatgt ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt
     1021 gatccaaacc aagtgacagg tgcttatctt tcaccgcaag aatttaaaga agctatttta
     1081 gatgaacaga cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt
     1141 cgaggggctg tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag
     1201 cacaaagaag aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc
     1261 gagaagtttt ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc
     1321 ggtattgcta cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg
     1381 tatgtgtttg atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt
     1441 cgcgactggt ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc
     1501 aatcgtcaga ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata
     1561 gagtgtgcga aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa
     1621 gcagaactgc ggtttacagc tttaagtaaa tagaaaacaa tagttttgat tgtcgaccat
     1681 ttccaatatg cgctatacta gcgttgagga tggtgatcta tatgaaatca ttactttatg
     1741 ctattgggga tgtccatggc aaatatgagc tttttgaaaa acttattcac tattatgatc
     1801 caaagatcca tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag
     1861 aatgtttttt attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa
     1921 accatgaaga atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata
     1981 tgagaaatgg tggaaaagaa acgtttgaaa gcctgcttca cccaggggca tcggaggaat
     2041 actccccaac tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt
     2101 tagtccagcg ccccttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg
     2161 atttgacaaa gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac
     2221 catttcatga agggaaaaac aatacaggta agacaattgt atttggacat acgattacac
     2281 caatgctcca tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa
     2341 tggacggtgg ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta
     2401 ttcttcaaga tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg
     2461 aaagaatgaa ggagctgtga ctttgttgcg gctcttttta tttgtataga gaatattgtt
     2521 tctttcagta gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca
     2581 gaaaagaggc attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa
     2641 agagctttca gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg
     2701 aaacactgtt ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt
     2761 ccagattcgt gaaatcgaag agcggtatgc atatcttgaa aacttagaaa aacgtaaaac
     2821 tgaagtgatt cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat
     2881 cacccaagcc gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg
     2941 acggacaaaa gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat
     3001 gcagcttaca gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaaa
     3061 agtctcttct gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag
     3121 cgataatgca aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt
     3181 cagcaatgtc aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt
     3241 tgcagaaccc gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa
     3301 agaagatatt ctgaaagtat tccttcaagt agatgaagca gcaattttag cttacttgga
     3361 tcgccaaata gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca
     3421 ggatagttat aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac
     3481 tgaaaaggca gatgagcaag caatcgctat tttcggtgaa aacctccgta atcttttgtt
     3541 gcaaccgcca ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg
     3601 taaactagca gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca
     3661 taaacctgca gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa
     3721 taaatatcaa gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact
     3781 ttttgtggct gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga
     3841 agcaggcgct tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca
     3901 agttgaagaa agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt
     3961 agtcaaaatc gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa
     4021 acgtttggct gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga
     4081 tgtgaataca gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc
     4141 ccaaaacatt gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa
     4201 aaaagtgcca cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc
     4261 tggtgggaaa aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa
     4321 agaaatatta acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga
     4381 aaaattgaca cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac
     4441 gttaaccgat attttagctg ggctgacaca acctggacga gatatgcgtg ttgagatgcc
     4501 agcgccctta ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt
     4561 gactggaacg gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca
     4621 agacggactt gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt
     4681 cgtgtcagta ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag
     4741 aatcagtttg acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga
     4801 cacaagaaga actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt
     4861 tcaagcacca agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa
     4921 atgatcatca tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg
     4981 caggaatcat caagcatgaa ctttgccatt atcatcttca tctagctgga agagggtatc
     5041 agcataaaga tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac
     5101 cagccttgac gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca
     5161 cgatctgccg caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatgggg
     5221 aaatcaaatg gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa
     5281 aggatggaag gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat
     5341 taaatttttt agccgcacct gaagagggaa attttgagga actgacggca cctacactta
     5401 ttttagctgg aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg
     5461 gccaagtaga tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg
     5521 agaattttga aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc
     5581 gtcagtatct aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt
     5641 ctacaaattc tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg
     5701 tactagaaaa aatcttattg atgaatgatc cgacactgca acgcaggact caagcaactt
     5761 ttgagaaagt atggaaaagt gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg
     5821 aaatccttca cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc
     5881 ctaaggaata tttccatgcg ctagtattag gagaaatgga acgactccat gacgatgaga
     5941 acggctatgg tcccaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt
     6001 ggtcttctta caccagaatt aaagagtcaa taaaaacgga tttttcccgt acttgaccat
     6061 tttagcaagc gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag
     6121 ataatgagga gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg
     6181 attcaaatgg aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg
     6241 agctccttgg ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa
     6301 aaatatttct ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac
     6361 gcagataatg taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat
     6421 gaagaaatcc agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt
     6481 ggtcatttta tccaaaacta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt
     6541 acgactggac tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga
     6601 gatggaacag gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat
     6661 cgccgtcatt tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat
     6721 catccggatt attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga
     6781 caagtgaaaa aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca
     6841 gatgtgccga tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaagatc
     6901 gaacgcacaa cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac
     6961 ttcactgtac cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta
     7021 gcttatttcg aaaaactgga tctgaaagaa gtggcagaag aaatgatgac tttcccagga
     7081 gtaaaacgtc gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg
     7141 catcaccctg cagagatcaa agcaacgatc gatggcgcac gccaaaaata tccagataaa
     7201 gaaataattg cagttttcca acctcatacg tttacgcgta cgattgcatt gatggatgaa
     7261 tttgcagaag cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct
     7321 cgggaagaac aaggaaacgt taagatcgaa gaccttggag cgaaaatcaa aaaaggtgga
     7381 gaagtcatca aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc
     7441 ttcatgggtg ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc
     7501 acgacgaaaa atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg
     7561 ggagtggcaa aaatcttgcc aagctccctt ttttgcaact tatgataaac ggcgttcaaa
     7621 atcttgcatc tgcggtaata agtccagcca aatcaaaaac atgagaagct gtttttcttg
     7681 gctgttctta ttacttgatg cagggcagct ttttgacgac ctctgtaaac tataggaatc
     7741 tgttaaatca agggagcaga acttgccttt tacgctactt ttggtacaat aatgtatgat
     7801 taaaaataaa ctggagggtt tctatcttga acataggtaa gccgagaaaa ctaggaaaaa
     7861 caataaatga aatcgaggaa ggcgactcgt tgtccttaac agaatctatt gaagataacc
     7921 agcttttgct gtatttggga ttgacgaatg atgcaaatcc tttgtatatc caacatgact
     7981 ttgcacaaaa aacagaatat caacagccaa tcgttccatc agtgatgctg atggggatca
     8041 tcacaagtgc aatttcaaag catcttcctg gaccaggatc acatgttgtc aatttttctg
     8101 tcaattttat tgaaccggtt ttccattatg aaacactgac ttttcagttt gaggtcataa
     8161 aagtagataa gatgaaggat gtggtgacga tttctgtaga agcggagaat acgaacgaag
     8221 atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca agttcctgaa ttggaaatag
     8281 caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta catcaatgtc aggtatcaat
     8341 cgtttttatg caaaagttta tggattttta ggtatgggac ttgcccttag tgcagtcact
     8401 tcttacttgg ctctttgggt atttcctttg caattagcaa cttttatcaa taattttcca
     8461 ctaggctttg tgggtctgtg gatcgttgaa ctgattttag taatggttct aggcgtaaaa
     8521 gcacaaaaga atccgagctt agcaatcggt ggatttattg cctactctct tttgaatggc
     8581 ataacattag cggttactct tgcatactac gatattggaa cagtaacgca agctttcttg
     8641 acagctactt tgatgttcat ggggatgtct gttgtcggag taacaacaaa aagagatctt
     8701 agcggggttg gaagagcagg acaggcagct ttaatcggag tgatcattgc actagtcttg
     8761 aacatcttca ttttgagaag tagcgcagtt gagctgttca tctccctact gttagtcgtc
     8821 atctttgcag ggatcacagc ttatgataac caaagcatcc gtaatatcta tgtccagaca
     8881 aacggaacag cacatagtgg aattgctgta ttcatggcat tgaagctgta tttggacttt
     8941 atcaacttgt tcttggcgtt tctgagaatc tttggacgga acaactaaaa aagaaaaaaa
     9001 ttcatcagtt ggggctatac cgaattattt ggtatggtcc cgctttttct tttcgcattc
     9061 cgcaggattc tttgttagaa tgaataagaa ctttacagta aggagttaag aagatgacaa
     9121 aaaataaatt attactcgtt gatgggaaca gtgtggcttt tcgcgcattc tttgcgcttc
     9181 acaattctct tgaacgtttc aaaaatagaa acggtcttca tacaaatgca atctatgcat
     9241 tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc gactcatgtt ctggttgcgt
     9301 tcgatgcagg aaaaacgact tttcggacag aaatgtatgc ggagtacaaa ggtggaagat
     9361 ccaaaacacc tggcgaattc aaagaacaaa tgccatatat acgtgatttg ttgacaggac
     9421 taggggtaca gtattacgaa ttgcccaatt atgaagcaga tgatattatc ggtactttgg
     9481 cagaaaaagt agacaaagat caatttgatg tcgtcgtgct ttcaggtgat cgggatttga
     9541 ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt aaaaggtgtc agcgatatcg
     9601 aaagctatac accagaacat gtagcggaaa aatatgatgg gttgacgcca aaacagatta
     9661 ttgacatgaa gggactagcc ggagatgcct ctgacaatat ccccggtgtg actaaaatcg
     9721 gtgaaaaaac agctatcaag ctattgaaac aatatggctc agtcgaagga atttatgaac
     9781 atatcgatga aatgaagcaa agcaaaatga aagaaaatct gataaatgac aaagagcagg
     9841 ctttcttatc gaaaaaattg gcgaccatca atacttcagc gcctgtggat gtctctatcg
     9901 actcattgaa gtatgaaggg aaaaatctcg ataaactcgt tccgttttat aaagaaatgg
     9961 atttcaatca gttcctttct aaattgaata tcgtagaaga agaagtaaaa atggatgata
    10021 ttttgtttga agtcgttcat gaattcaaag aggaaatgtt tactacggat atggcgttgt
    10081 acgtggaaat gatgggcgac aattatcata cagaggaaat cgtcggagta gcgtggggga
    10141 cagaaaagaa aatttatgtc acgaacgatc tatctgtatt cgaaaaccga gcgttccatt
    10201 cttggatcac tgatccgact cggctgaaaa aagtatatga tgcgaaacga acttatgttg
    10261 ctttaaaccg ctatactgga aaaacgaaag gaatcgattt tgatgtcttg ctaggtgcat
    10321 accttctcga tacgaatgaa aaaagcacag atatcgaagg aatagctgct cattacggat
    10381 acaacgatat ccaatcagat gaagctgtat acggcaaagg ggcgaaaaaa ggtttaccag
    10441 aagaagagga actctttttt gctcatttag cgcgaaaagt agctgcaatc aatgcgttga
    10501 caccaaaatt gagccaagaa ctggcagata aaaaccaaga agatcttttc cggaaaatgg
    10561 aactgccatt gtctatcatt ttggcagaga tggaaataag cgggatcaaa gtagatgcga
    10621 ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt aagagagatc gaacaaaaaa
    10681 tctacgaaga agccggcgaa gaattcaatt tgaattcacc aaagcaatta ggagtgatct
    10741 tatttgaaaa aatgggatta cccgttatca aaaaaacaaa aactggatat tccacagccg
    10801 tagatgtact ggaacagctt cgtgaacaag caccaatcgt agaggatatc ctcacctatc
    10861 gtcaaattgc aaaaattcaa tctacctatg tcgagggact gctgaaagtg atccaatcag
    10921 atggaaaagt gcatacccgt tatgtccaga cattgaccca aacaggacga ttaagctctg
    10981 ttgatcctaa tttgcagaac atcccgattc gattagatga agggagaaag atacgtcagg
    11041 catttgttcc tagagaaaaa gactggctga tttattcttc tgactattca caaatcgagt
    11101 tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa agctgccttc ctagaaggtc
    11161 aagatatcca tgccagcaca gcgatgcgtg tatttggaat cgaaaaggca gaggatgtca
    11221 caccaaatat gcgtaggcag gcaaaagcag tgaattttgg tatcgtgtat ggcatctctg
    11281 attacggact ttcacaaaat ctaggaatca gccgaaaagc agcacagcaa tacatcgata
    11341 cgtactttga aaaataccca ggtgtcaaag aatatatgga aagaatcgta cgcgaagcaa
    11401 aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg atatctgcca gacatcaatt
    11461 caagaaatta caatatccgt tcttttgcgg aacgaacagc tatcaataca ccaatccaag
    11521 gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt ggataaacga ttaaaagaaa
    11581 caggtcttca agctacgatg ctgttacaag ttcatgatga acttgtcttc gaggtaccgg
    11641 aaaaagaact tgaatcatta gataaattgg tgaaggaagt tatggaacaa gctgtttctc
    11701 ttcatgtacc actgatcact gacagcagtt ggggaaaaac gtggtacgaa gcaaaataaa
    11761 cattgtttaa gacagagcaa tataatgaag gtgaaaagaa aaaatgccag aattaccaga
    11821 agtagaaacc gttcgtaaag gattggaacg gttagtagta ggaaaaacga ttcaaaaagt
    11881 tcaggtcttg tggcccaaga tcatcgagca accagagaca cccatctttg aggcttctct
    11941 tgttggcgaa accatccaat ccattggacg tagagggaag tttttaattt ttcatctcga
    12001 tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa tatcaattta cgaaagaaaa
    12061 tacaccaatc gataaacata cgcatgtact tttctttttt gaggatggaa gccaacttcg
    12121 ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc gaaaaaggag caagtgcaac
    12181 ttaccgagga atcatgaaat tgggacctga gcctttgcca gatagcttct tattagcaga
    12241 tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag ccattgctgc tagaccaacg
    12301 tctagtgact ggtctaggta atatttatgt agatgaagca ttatgggaag cgaagatcca
    12361 tccagaacaa ccggcgaaca cactgcgccc caaagaggta gaacaattac gtctatcgat
    12421 cattgatgtt ttggatcgtg caatagaagc cggaggtacc acgattcgca gttatttgaa
    12481 tgcattagga gaatcaggcg gatttcaagt agcactgcat gtctatcagc aaactggcaa
    12541 accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa gtggcacaaa gggggacgca
    12601 ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag gatttgtttt aggtttgacg
    12661 ggtggaatag ctaccggtaa aagtacggca gccaaagtct ttcagtctca tggatttcct
    12721 ttgatcgatg gcgatgtgat tgccagagaa actgttgagc ctggaacgct tgctctgagg
    12781 aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg atgggcaact agatcgaggg
    12841 aaacttggga tgattgtttt tccatcaaag gaaaaacggc aaaaacttga tcagctatta
    12901 gatccgttta tccgaaaagc gataaaagat caaatcgcct ctctaagttc caatcatcct
    12961 ttagtaattg tagatatccc attgctctac gaaggtcatt atgaccacta tatggatgca
    13021 gtagctgtcg tttatactac gccagaaact cagcttcagc gtttgatgaa acgtaatcaa
    13081 ctgacgctag aacaagcgca gcagagaatt agtagtcaac ttccaatcga agaaaaaaag
    13141 cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag aaaaacttgt gggacaaatc
    13201 gaaaattggc tttttgctaa tcgctttctt tgagtatctg tgtcaatttt gctatcctga
    13261 gtatatagaa agtgagggat agcgatggga tcattaaaaa atcaaaagga taaagtcgta
    13321 ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag aaaatctaat cgatgaaggt
    13381 cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt accaagcaag aatcgatgaa
    13441 atagaagacc agcaggcaga agaagaagcg aagcggcaag atttgactca agaacgaaaa
    13501 aatccgacac ctgatcgtag taaaacaccg ctcccagaaa gtgaccacat tcccgatgag
    13561 caaaaaatcg aggaacagcg aatgaaacac tatacaggaa tcgaagagaa gttataaaat
    13621 agtaaaaaat cgagatgtga cttgaagcag atgagaaggt gttcatctgc ttcaagtttt
    13681 ttgtgctaga tgactaaatc agcttttgtg taagttattt ctcagtaatc aaggtgatca
    13741 gtattggaaa attgaaattt agtaaggaca ggggacacaa ttacgcatga aagtagaaaa
    13801 aattgttctt atatccgtgt tttttgggta ggacagtcgc gtttgtgatt tgtttcgtgt
    13861 tataatacaa ctagttgtaa agaaagacac atcctttata taaaatgcga tgcaagaggt
    13921 gagacaaaat gagatgtccc agatgtcaac ataataattc acgagtaatc gacagccgtc
    13981 aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg tgagaattgt tcgttccgat
    14041 tcactacatt tgaacgaatt gaagctgctc cgttattagt aatcaagaaa aacggggaac
    14101 gcgaagagtt caatcgcgat aaaattttaa gaggactcat ccgttcagca gaaaaaagac
    14161 ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt cgaaaaccgc gtacgcagtc
    14221 tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata tgtgatggaa gatttagttg
    14281 atttagacga aatcgcttat attcgttttg caagtgttta tcgccagttc aaagatatgt
    14341 ctgttttctt aaaagagttg caagatatcg tggataaagc aaaagaaaat aataaagaga
    14401 ataataaaga aaacaattaa gaaacaaaaa ggaggcattt ccttggaaaa tgcttggaaa
    14461 gagcttcaac caaaaaatat ttaccaagga agaaaagcgg ctccgctgag agaagacagc
    14521 aagaacgcct tgctgtatct ttatcagcca ctaatcggcg gagaagcctt gagtatctat
    14581 ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag gtcctgaagg gatgcatgca
    14641 gatctgctta gtagtctagg ctgcggtatc ccgcaatttt atgatgcaag aaaaaagcta
    14701 gaaggaatcg gcttactgga tgtttatttt aaagaagata gtgatctggg acctcgtttt
    14761 atttatgagc tgttagaacc actcattcct aaagcatttt tcaaagactc tgttctggct
    14821 tttcttcttc tggaaaaagt tggtgaacga cgattccaga agcttaccca attctttgaa
    14881 cctaaaaggc atacagcaga gggatatcaa aaaatcacaa aaaaattttt agatgtttat
    14941 cgcttcaatg aagcagtgta cgctgctaat caagaaaaca tcgaatcgct gaataaatct
    15001 tttgaagaat ctgaatcagt agcattactt gaagcagatt cgacattcga ttggcagttt
    15061 ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg atgatccaat catcaaacaa
    15121 gtcgacatgt atcatcagct ttacggtttc gatgaattga cacttggcga actgatcagt
    15181 caatcttatg actttagtga acaaaaagta tctgaaaaag aactgcaaaa agccattttg
    15241 gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag ttgcggtttc tgaaaacagt
    15301 aatgaaccct cgtcaaacga acatttgaca aacgcagcgc ttcagctgat aaaagaagca
    15361 cagagcattc caccgatgaa atatctggaa gcaatcaagt cagaaaaagg cggatatgtt
    15421 tctaaacaag aaaactggtt actgcaagat cttgtttctc aatcaggtct ttccagtagc
    15481 gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga ataatgctag cttaaatgcc
    15541 agttatgtaa atacagtggc aaatgaatgg gcacaaaaga aaatcgccac agcagaagaa
    15601 gcgatccagc acattcggca aataagtaat caagcaaaac aatcaaaaca aaagaagcaa
    15661 acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa agcttcctga ttgggtcaac
    15721 cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag cagagattga ccgccgtttt
    15781 aaagaatacc tggcaaaaaa ggagggtgat atctagtgga agacgtaggc aaagaattga
    15841 agaggatcat cagcaaacgc aattatcaag aaaagtatgc tgaaatgatc gctgaagtct
    15901 taaatgatgc ggatgttcaa gcttttctaa gcgcacacca agaagaattg acacaagcgg
    15961 atattgaaaa aagttacgct aaattgtatg aattcgtgca agaaaagcgt aaatatcaaa
    16021 aaaacgatcc aacgatgatc gctcccggct atgagccgaa gctgacgttg aattttcatt
    16081 atgtggatgt cacttatgta ccaacggaag cattgttagc acggcaaaga caagaagaaa
    16141 tcagaagccg agtaaaagca atggatatgc caaaagatat tcaatcggct tcttttgaaa
    16201 actttgaacg tacagatgga cgaggatatg cgagcttaga agccctagat tttgtagaga
    16261 aatatactgc tgatccaaaa gggtatcata aaggactgta tctagtagga agttttggta
    16321 ttggaaaaac atatttatta ggagcaacag cgagagaatt agcgattgct ggattcacca
    16381 cgacgctagt ccattttccg acatttgccg ttgagatgaa acaggcaatc ggaaaagatc
    16441 aagtggcaga aaagttagat gcagtgaaac gttcacctat tttgatgatc gatgacattg
    16501 gtgcagatgc gatgagtagt tggatacgag acgaagtatt cggtgttatc cttcaatatc
    16561 ggatgcagga acagttacca accttcttct catcgaattt tacaatggat gaattggaac
    16621 agcatttgtc cgtcactcaa cgtggagatg aagagccgct aaaagcgaaa cggataatgg
    16681 aacgcatacg ttatttgaca aaagaaatcg aaatgactgg tcgcaaccga cgcaatggtt
    16741 gaacagttga caagtccaac tgacatttga cctagtagta aaagcaggtc agcgaatcat
    16801 tatatggaca gaaatgaccg gagacaatca ccgcaacaag taatgcagaa acttaaacgg
    16861 aggatttttt atgagggcta ttttttctag tatatctgta gttggtctat tttttctttt
    16921 agcaggctgc ggtagcgcag atagcaacac aggttctgtt gattcgattg atgcgggtca
    16981 aacagtaaaa acaagcagta cgaaaacgga gagacaaagc atatttgtcg gaacgattga
    17041 aagtatcact caagtagaag cagaagaaga aacgcttcag ttgaaactcg tcgatgtcat
    17101 ggccaaagaa gatccagcga atatgggaac gtcattcagc aatgacggag tgatcttaaa
    17161 tgctactgct gaaaaaataa gcgtggacat aaatgaattg aaaatcggtg acaaagtcca
    17221 atttacatta gttgagatgc cgatcatgac gatgtctatc ccaccgcaag ttcctggaga
    17281 ttcgattatt gatgtttcat tgaacaaata accaataaca ttttttcaaa aataagaaaa
    17341 tgttgtttta tagaacgaaa aggtatattt tttaaggaga tatgggaatt tt
//
LOCUS       DAYYNG010000051        17306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000051 DAYYNG010000000
VERSION     DAYYNG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.051"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1500
                     /locus_tag="ACHBFA_001354"
     CDS             <1..1500
                     /locus_tag="ACHBFA_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM9394856.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDD
                     TLLEVQNDKSVEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="ACHBFA_001355"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="ACHBFA_001355"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9394857.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="ACHBFA_001356"
     CDS             3076..3351
                     /locus_tag="ACHBFA_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9394858.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="ACHBFA_001357"
     CDS             3357..4124
                     /locus_tag="ACHBFA_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9394859.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="ACHBFA_001358"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="ACHBFA_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9394860.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="ACHBFA_001359"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="ACHBFA_001359"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM9394861.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="ACHBFA_001360"
     CDS             8268..8570
                     /locus_tag="ACHBFA_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM9394862.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="ACHBFA_001361"
     CDS             8573..9736
                     /locus_tag="ACHBFA_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9394863.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="ACHBFA_001362"
     CDS             9762..13190
                     /locus_tag="ACHBFA_001362"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM9394864.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="ACHBFA_001363"
     CDS             13211..14335
                     /locus_tag="ACHBFA_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM9394865.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14316..14762
                     /locus_tag="ACHBFA_001364"
     CDS             14316..14762
                     /locus_tag="ACHBFA_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9394866.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="ACHBFA_001365"
     CDS             14746..15084
                     /locus_tag="ACHBFA_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9394867.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="ACHBFA_001366"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="ACHBFA_001366"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9394868.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="ACHBFA_001367"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="ACHBFA_001367"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9394869.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="ACHBFA_001368"
     CDS             16241..17284
                     /locus_tag="ACHBFA_001368"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9394870.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5867 a         2998 c         3831 g         4610 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggt
//
LOCUS       DAYYNG010000052        16391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000052 DAYYNG010000000
VERSION     DAYYNG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.052"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1063
                     /locus_tag="ACHBFA_001369"
     CDS             <1..1063
                     /locus_tag="ACHBFA_001369"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM9394871.1"
                     /translation="IISVQTGMVDKPNERRINKVPMPSAGGLPIFISFVISTLFLFRK
                     IIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGILIASLIIYFVADIRIDSFTL
                     PFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAAGISLIGLITIGIIGYFFLHA
                     STVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALFLGFMMAVLSLQGLKNVTFVS
                     SISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLHHRLISLGFTHKGAVLTIYAM
                     ALMFSFTAMVMNYTGRIGTIILIIAMLFAAILLFELIGLINEKHQLILKTLRFLGNKE
                     YRTQVKQKYLKKWNRHIDE"
     gene            1056..1856
                     /locus_tag="ACHBFA_001370"
     CDS             1056..1856
                     /locus_tag="ACHBFA_001370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9394872.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1856..2683
                     /locus_tag="ACHBFA_001371"
     CDS             1856..2683
                     /locus_tag="ACHBFA_001371"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9394873.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2688..3395
                     /locus_tag="ACHBFA_001372"
     CDS             2688..3395
                     /locus_tag="ACHBFA_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9394874.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3495..4361
                     /gene="rfbA"
                     /locus_tag="ACHBFA_001373"
     CDS             3495..4361
                     /gene="rfbA"
                     /locus_tag="ACHBFA_001373"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9394875.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4375..4947
                     /gene="rfbC"
                     /locus_tag="ACHBFA_001374"
     CDS             4375..4947
                     /gene="rfbC"
                     /locus_tag="ACHBFA_001374"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM9394876.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            4969..5997
                     /gene="rfbB"
                     /locus_tag="ACHBFA_001375"
     CDS             4969..5997
                     /gene="rfbB"
                     /locus_tag="ACHBFA_001375"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9394877.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6095..6946
                     /gene="rfbD"
                     /locus_tag="ACHBFA_001376"
     CDS             6095..6946
                     /gene="rfbD"
                     /locus_tag="ACHBFA_001376"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9394878.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            6981..9014
                     /locus_tag="ACHBFA_001377"
     CDS             6981..9014
                     /locus_tag="ACHBFA_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394879.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9058..10338
                     /locus_tag="ACHBFA_001378"
     CDS             9058..10338
                     /locus_tag="ACHBFA_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM9394880.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10548..11354
                     /locus_tag="ACHBFA_001379"
     CDS             10548..11354
                     /locus_tag="ACHBFA_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9394881.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11366..12586
                     /locus_tag="ACHBFA_001380"
     CDS             11366..12586
                     /locus_tag="ACHBFA_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394882.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12576..14162
                     /locus_tag="ACHBFA_001381"
     CDS             12576..14162
                     /locus_tag="ACHBFA_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9394883.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14201..16339
                     /locus_tag="ACHBFA_001382"
     CDS             14201..16339
                     /locus_tag="ACHBFA_001382"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9394884.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         5198 a         2716 c         3494 g         4983 t
ORIGIN      
        1 aatcatttca gtacaaacag gtatggtcga caaaccaaat gaaagaagaa taaataaagt
       61 tccaatgcct tcagcgggcg gactgcctat ctttatttca tttgttatat cgactttatt
      121 cttattccgt aaaattatcc ctcaatcgta tattgtaccg atcttgattg cttctttagt
      181 aatcatagtc acaggagtgc tcgatgataa atatgagctg acacctaaac aaaaaagtat
      241 agggatcttg attgcttctc ttattattta ttttgtagca gatatacgta ttgattcctt
      301 cacgcttcct tttattggat atatccaact aggctggctt agttttcccg taacgatttt
      361 ttggatcttc ggtatcacga atgcggtcaa tctgattgac ggtttggatg ggctggcggc
      421 agggatttca ttgatcggtt taattacgat aggaatcatt ggatattttt tcctccatgc
      481 ttctacagtg tatattccag ttgttatttt ctgtcttgta gcaagtatta tcggtttttt
      541 tccttacaat ttttatccgg caaagattta tttaggcgat acaggagctc tattcctagg
      601 gtttatgatg gccgtccttt cactacaagg gctgaagaac gtgacttttg tttcatcgat
      661 ttctttgctg atcgtcatgg gagtgccggt aacagacacg ttttttgcta tcattaggag
      721 aaaagcaaac cgtgtttctt tttctacagc agataaaaaa catcttcatc accgactaat
      781 cagtttaggc tttactcata aaggtgctgt cctgacgatt tatgcgatgg ctttgatgtt
      841 ttctttcaca gctatggtca tgaattatac tggaagaatc ggaacgatca tattgattat
      901 tgccatgttg tttgctgcaa ttttattatt tgaattgatt ggtctgatca atgagaaaca
      961 tcagttaatt ttaaaaacat tgcgattttt agggaataaa gagtaccgta cgcaagtaaa
     1021 acagaaatat ttgaagaaat ggaaccgtca tatagatgaa taacaaatta aaccagccga
     1081 tccttattag tattgtgacg gcaaacagtg ataaaatttt tcggacacta gataatttta
     1141 tttcaactgt aaaagatgaa acagcagtaa aaatccgaat ttttgacaac aattcacttc
     1201 ccgagtacaa aaaaagacta acagagtata ggaaatatcc ctacatcgat atctattttc
     1261 atgatgaaaa ttccggattt ggttatggac ataatcacaa tctgatagct agcgattctc
     1321 gctatggcat tatttgtaac ccagatattt tagtggatga agagacagtt acagcattgg
     1381 tcttgttatt gaaagaacat ccggaatgtg cgatgttagc acctaagatt cttaatgaag
     1441 atggaacaac tcagcacctc atccgtgagc agctggctgt ttttgattac gttttgcgct
     1501 ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg tttgaatgcc
     1561 gaggattgcc tactgatgct aatagttatg tacggatgat ctcaggtagt tttatggtag
     1621 ctgatattga taaatttaaa gagataaagg ggtttgatga gcgtttcttc atgtactttg
     1681 aagacaacga cttgtgtttg agttttgaaa aagcaggtta caagttattg tacacaccct
     1741 tttatgcagt tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa ctgtttcgcg
     1801 tgtttttgac ttctatgatg aaatttttca ataagtgggg gtggcgtttt ttctgatgaa
     1861 accagaaata ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat ctccagctta
     1921 tggaccatta agcaaagcac ttagcgaaaa gcaaattcag cttattattt acgataatag
     1981 tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat atcgtttatc atcatgatcc
     2041 gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg tctcaagcag atgaaggaac
     2101 acgttgtctc gtcactctgg atcaggatac gaggctagtc gacgattatt ttgaactatt
     2161 gcgtaaagta acctttacag atgaatgtgt tgctgcagtt ccgatgattt tttctgggag
     2221 ccgacaagtt tctcccctat attcagatcg ttatatcaat cgtacagctc aagcagtaga
     2281 agttgaaggc ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg cctggtctgt
     2341 caaatttcta aaaaagattg gcgggtttaa tcctgctttt tctttagatt ttttggatca
     2401 ttggctgttt tggaaagtgg atcagttgga aaaaactgtg gagatccttc ctgtacgaat
     2461 ggaacatgat ctttcggttt tagactatga aaaagtttca agtaatcgat accgttctat
     2521 tttacaagca gaaaatcgat tttatcatga atatgatcaa aggcatttat ccagtcaccg
     2581 acgacaatta ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc ggcatatctg
     2641 gcggatgact ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg cgttcggttt
     2701 gtatagctac ctttaatgga ggcagatatt taagagagca gttagatagt attttatctc
     2761 agctttcagc agaagatgaa gtagtcattt ctgatgatgg gtcagtagat gatacatggg
     2821 atatattaac tgaatacgca gaaaaagata agcgaatcca gcttttttcg gggcctcgtc
     2881 aaggattgat cgctaatttt ggttatgcga tccagcagac gaaaggagat ctaatctttt
     2941 tggcagacca agatgatgtt tggttggaga caaaagtgaa aaagatcact gactattttg
     3001 agcagaatcc ggatcagcta gtagtagttt ctgatttaat cattgtggac agcgagctgc
     3061 aagagatcca tgattcgtat ttttcgtttc gaaattccaa agaaggatgg cttaataatg
     3121 tgataagaag tcactatatt ggtgctggta tggctttccg gtcttcttta aaacccgata
     3181 ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg gattggtttg ctgggaggaa
     3241 agaagactgg gttcctaaga gaaccgttaa ctctgtaccg aagacatgat ttaaacgcga
     3301 gtgagattga aacacattct aaattacggc aaaaaatttc ttggcgtgtt catctgatta
     3361 cggcgttgat caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa tccttaatat
     3421 ttccatgaca aatataacaa ttgatgagat aataacaaag tgaaatttga aatcaaaaag
     3481 gagagcagat atccatgaag ggaatcattc ttgctggggg cagcggaaca cgtctgtacc
     3541 ctttaacaaa ggcaacatcg aaacaattga tgccgattta tgacaaacca atgatttatt
     3601 atccaatgtc tactctgatg ttggcgggaa tcaaagaaat tctgattatt tcaacgccac
     3661 aagatacacc acgtttcaag gaattatttg gaaacggaaa tgatttagga ctgcagatcg
     3721 aatatgcagt acaagaaagt ccagatggat tggcacaagc gtttatcatt ggagaagaat
     3781 ttattggaga tgatagtgtc tgcttggtat taggggacaa tatttactat ggtggcggtc
     3841 tgtctaaaat gttgcaacga gcagcttcaa aagatacagg cgcgacagta tttggctatc
     3901 atgtcaatga tccagaacgc tttggtgtag tagaatttga tgagaatatg caagctttgt
     3961 cgattgaaga aaagcctgct cagccaaaat caaattacgc tgtaactggc ctttattttt
     4021 atgacaatga ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt ggagaactag
     4081 agattacgga tgtcaataaa gcatatttgg ataaaggaaa actatccgta gaacttatgg
     4141 gacgcggatt tgcttggctt gatacaggaa cacatgaatc attgcttgaa gcatctacct
     4201 ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa gaaattgctt
     4261 accgtatggg ctacatcgat aaggaacaat tgctggccct tgcacagcca ttgaaaaaaa
     4321 atcaatacgg tcaatattta ttgaatttgg ccgctgaata aggagtcctt acagatgaaa
     4381 gtaacagata caaaattaac agatgtcaaa atcgtagaaa tggacgtctt tggcgatcat
     4441 cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg attgaaatat
     4501 gactttgttc aagacaacca ttcactatca gctgaagctg gtgtccttcg ggggcttcat
     4561 ttccaaaaag gagaagctgc acagacaaaa ttgattcggg tagtcactgg agcagtttta
     4621 gatgtagtag tggatatccg caaaggtagc ccgacttatg gtcaatggga cggatttatc
     4681 ttatctgaac ataatcatcg acagttgctt gtacctaaag gatatgccca tggattcgtt
     4741 actttgacac caaatgtgaa cttcctttat aagtgtgata attactataa tgcagaagca
     4801 gatgggggca tcgcatttga tgatccagat ttagcgattg attggccaat tgatccatca
     4861 agagcgatta tgtcggaaaa agatcaccgt catccgacat taaaagaatt tgaagcagaa
     4921 aatccatttg tttacggaga aatctaagaa ttaaggagaa attttcttat gaaaaacatc
     4981 attgttaccg gaggtgccgg attcatcggc tctaatttcg ttcattacgt ggtaaacaat
     5041 catccagaag tgcatgtcac tgttttggat aaactgactt atgcaggaaa caaagagaac
     5101 ttggccggac ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc agatgcagaa
     5161 ttagtggacc gtctagtaaa agaagcggac gcagttgttc attacgcggc agaatcccat
     5221 aacgataatt cattaaaaga tcctttccca tttgtacaaa cgaatatcat tggtacgtat
     5281 acacttttag aagcttgccg caagtatgat gtacgctatc atcacgtatc tactgacgag
     5341 gtttacggcg atcttccatt acgtgaagac ctgccaggac atggggaagg cgaaggagaa
     5401 aaattcactg cggaaacacc atataatcct tcaagtccat attcttctac aaaagctggt
     5461 tcagatctgt tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac catttcaaac
     5521 tgttcaaaca actatggtcc ttatcagcat attgaaaaat ttattcctcg ccaagtaacg
     5581 agtatcttaa gcggtattcg tccaaaatta tatggcgaag gaaagaatgt tcgtgactgg
     5641 atccatacga atgatcattc ttcagcagtg tggctgattt tgacgaaagg cagaattggc
     5701 gaaacctatc tgatcggtgc agacggtgaa gaagataata agaccgtgat ggaattgatc
     5761 ttagaaatga tgggccaacc aaaagacgca tacgaccatg taaacgaccg cgcagggcac
     5821 gatttgcgtt atgcaatcga ttcgacaaaa ttgcgagaag agctaggttg gcagcctgag
     5881 tttacgaact tccgtgatgg gttggctgac acgatcaaat ggtacgaaga acatgaagac
     5941 tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg acaataatac
     6001 agaggaaatt tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc gcctgtctct
     6061 tttttaaaat aaaaatcaag gagcggataa ccatatgttt ttattaactg gaggaaatgg
     6121 acaattaggt acagaattgc gccatttatt agatgaaaaa aatctgccat atgtttcaac
     6181 agatgcaaaa gacttggata tcacagatgc tgagaaaaca atggcttata ttacagaatt
     6241 aaaacccgat gttattttcc attgcgcagc ttacacagca gtagataaag cagaagaaga
     6301 agcaaaagaa ttagatgaga aaatcaatgt cgatggcaca agaaatgtag cacttgcagc
     6361 aaaagctgtg aatgcacaat tcgtctatat cagtacagac tatgttttcg atggaaaaaa
     6421 caaaaatgta gaatacgaag tagacgacca aacgaatcca ctaaatgaat atggtcgtac
     6481 gaaattatta ggcgaaaaag cagtagaaga gattttagaa gactattata tcatacgaac
     6541 ttcttgggtt ttcgggatct atggacacaa tttcgtcttc acgatgcaac gcctagcgga
     6601 aacccgcgac aaactgactg tagtaaatga ccagtttggg cgaccaactt ggacacgtac
     6661 tttggcagaa tttatggtat atgtcatcga agaaaaagca ccatttggta tttatcattt
     6721 gtcgaacgaa aatagctgtt cttggtatga gttcgcaaaa gaaatattga aagatacaga
     6781 tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa aaagcaacac gtccgcagta
     6841 ttcagtcatg agcctgaaaa agacagaagc gttaggtttt gtcataccaa cttggcaaga
     6901 agcacttgcg caaatgctgg aagctgccaa aaaaatgaat aagtaatttc ctttttatca
     6961 aatagaattt ggagcatgat atggaaaaac aaacaagaag tgcacgacga aaagaaaagc
     7021 caaaagcgaa tcaggcagat tcgaatttgg tgcttgacct gtttctaaaa ctccgctatc
     7081 ttattggcgt tttagtcatc gtggtcatcg ttacttttaa tttaaacgga agttcacttg
     7141 gtgtttggga taaatatgtg tcgcaaagag acgatgggaa aaaaactgat gtgatttttg
     7201 gacagaatcg ggaagtgcgg tcagacgaat ggctggtgca gacacctttt tacctctcac
     7261 aagctgaaga ggacttttcg ttggtgaacg aggattattc attaaacgga caaaacatga
     7321 tcattgcgta taattctcct gtaaaggata ttacggttat cggaaaacca ttcaactggg
     7381 gattcttttt cctcggaaga gatcgaggac tttcctttta ttgggcgatg aaattagtcg
     7441 tcatggtctt gcttggattc gaagtactga tgatattaac gaagaggaaa aaagctctat
     7501 cgctaatcgg tgcatttgcg ctgacatttt ctccagcagt ccagtggtgg ttcatgcaac
     7561 atgtgggcga tttgattttc tttactttgg gactgatgat tgctttttat cattatttct
     7621 acaatcatga taaaaaatgg ctacgaacgt tgatgatgct tttggtggtt attttcggta
     7681 ttgggtttat tttagtgatt tacccagccc atcaagtgat gctggcgtac ttgctgattt
     7741 tttattttgt tggcctgctt atttactata gcaagaaagt ttcatgggat ctgttggatg
     7801 tagtgttgat tggaggggca gttttattta ttggcggagt catggttcat ttctggctaa
     7861 cgtcaaaaga cgcattgtta gcttctttga ataccttata tccaggaaaa cgggtaagta
     7921 ctggaggaaa gtggacgatt ggcgaatttt tttctttcct tacaaattgg aaaattcctt
     7981 ttgaagatat caactattcg aataactctg aagtggccct gttctatcac ttttttccaa
     8041 ctgtattcct agcaagtcca tttgtgttat ttggcaaaaa agatagcgaa caaaagttac
     8101 tgggacgtat tttgatgatt ttttgtctgt tttctatctt ttggattacc gttgggttgc
     8161 caaaatggct agcagaaatc actctgctgt cttatgttcc tccattaaga gccatcctta
     8221 cttttagttt tgcggcttgt ttattatcga tctggtttat cgcttatatt tggagagaag
     8281 atgttattcc tatttggtat aaattcattt tgttggcagt aaatgcatgg tcttatttct
     8341 atgcgattac gcattcgaaa atggataatt atttttcgga ttttgaaacc atcgcagttg
     8401 ctgtgttggg aacagtgatt ttagcttttg cattatttgg ctggagacgc ttgttttacc
     8461 tcgcttttgt cgccttagtt atcgtatctg gctttttcgt caatccagtc gtagaaggta
     8521 caggagcgat ttttgaaaaa acactggcaa aagaaatcca aaaaatcgat caacaagatc
     8581 ctggtaaagt ttggttgtct gaagatgaat tatataactt caccccaaca ttgggagtga
     8641 aatccttcaa tacagtacgc ttttatccag atatagctgc atggaagaaa atcgatccga
     8701 aagaagaata cgagaagtat tataatcggt atgctcatac aagagctttt atcgctaagg
     8761 aagatacaaa gttcatttta gatcgtccag atatgttttc ggtcacgttg aattttgaag
     8821 acgcagaaaa attagggatc gactatgtga tcagtaaacg accattaact gattacaacc
     8881 aattatatga agcgcagttc acgcagctat atggaccgga taaagacggt tatatgattt
     8941 ttaaagtaga atatccggat tatccagatg taccacaaac acaaactcag gaaaatcagc
     9001 tagtcgtaca gtaataggct agctgagaat aatcctgaaa tattagtaga aaggagagtg
     9061 aagataattc aagcaatcgt acaaaaattt gtcaacaaaa tgagtgtcgg tttcttgctt
     9121 ttgttgctga cgctagtctt ttttgtttgg acagcagggg taggttttcc agcaactttg
     9181 tttatatata acaatagttc attacttatc gcttttagtg tggctatttt gctcatttta
     9241 aatgtttata aagcaaaagg cgtcgatttt ctttatctag gtgttgcgct tgcccttttc
     9301 attttctttt cttactttcg ggaattaaga aatgagtctt tatttggaga cttactgttt
     9361 cctttgatta ttttattttg tttagtaatt aaatggatcg agtggacaaa atgggatcgc
     9421 ggtatttatc tgattgtctt tacggtcttc ctcggaatca cgttatatcg tgtatttaca
     9481 gagatccaag taccagaagg acaaagcatc tgggctccag gcaattcgct ttctgatatt
     9541 tggatcaata cgaatacaat cggaagttct ttgatgactc ttgctttatt gatcagtgga
     9601 tttgccagtt cttttgaacg atggtatgtt cgaatcatcg gtgggatagc agtcgttgca
     9661 gcctttttag gtatttgggt ctgccaatct agaggtgcat tagtcgcgtt gattgttttt
     9721 gcgatattgg atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc atttattgcc
     9781 tacacggtta cgattctttt agctttgccg atttcttatc tggcggctgt ctctgaaaaa
     9841 gtcaatttgt ttaccggtcg agaagatatt tggcataaat tctatcagac gctaggagaa
     9901 aaatctgaac aagttttact aggaatgaaa acatttatct tccagcgagg aaaccaattt
     9961 ttaggaaacc acaatagtta taattctatt ttgaatatct atggattgat aggatttggg
    10021 attgctgctt tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc agatttatcc
    10081 aacgggcaga tgacttttat ctgggccttt tttgcggtga tgatgcagtc atttatggag
    10141 gatacattga cttcataccc atgggttccg attatttata tcctgttggc tatggccgtt
    10201 catcgatatg atgaaccaga agttacggag gaattctatg ttatggaaga ggcatatgac
    10261 gatatgactt atgatgatac atcttatgag gaacaaccag atagcgctca ttcttccagg
    10321 atccaacgct atcattgata ggcacaaaat acgagagagg ctgtgacaac cgtcgcagcc
    10381 ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc
    10441 ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa aatatggtaa
    10501 aatggcactt gactgtaatt cactgaaaat cgcttggagg aacacaaatg tttaaagatt
    10561 tgtatttatt tatgaaggac atttatcaga acagaaaatt actgctacag ttttcgatca
    10621 atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc
    10681 cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga
    10741 tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct
    10801 tctcagatgc atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga
    10861 aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc
    10921 atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag
    10981 caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga
    11041 cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg
    11101 tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag
    11161 gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt
    11221 catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt
    11281 tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt
    11341 cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac
    11401 aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt
    11461 taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa
    11521 aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat
    11581 cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc
    11641 tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt
    11701 aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat
    11761 tgaatttgct gacatcggcg accatattta tcaacctgta aaaacctatt caagtgggat
    11821 gttcgttcgt ctagcttttg ctgtagcgat caatgtgaat cctgatattt tgattgtgga
    11881 tgaagcattg gcagtaggag atcttgaatt tcaactaaaa tgtatggaaa aatttacaga
    11941 aatcaagaac tcgggaaaga cgattttatt cgtttctcat gatattagtt cgattcgccg
    12001 tttttgtgat cgttcctatt ggttgaaaaa tggaaaagtt gtcgaatatg gtgatacgat
    12061 ggaagtaacg acgaattacg aaaacttttt gaagaaaaaa tcagttaaaa cagtagatcg
    12121 aaataaaaac caagtagaac gcttcgcagc tccagatatc gtagacgtga cgaaagccga
    12181 attgttggat aaagataaaa atccagtgga tatgctggaa caaggagaaa aactatacgt
    12241 taaagtaacc tatgaagtaa aagacgatac aatcaaaaat ccagttttag gaattgctct
    12301 gcgtacagtc gacaattttt atgtgtgcgg actcaatact ctactagatg gtatcaagat
    12361 cccttggaaa aaaggtgaga atatctttta tctggagtat gaaaagttag gtttgttagc
    12421 tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata
    12481 caagacaaaa tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt
    12541 cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc
    12601 tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt
    12661 tagagtttgg tcctggaaac ggccgattaa cgaaacattt gatcggagca aaacaatgtg
    12721 aagtgagcat tgtcgagcta gataaggaat tgttcgattt tgtcagcgag tttgcccaag
    12781 atggatttta tggagatatc gaaagttttg aatgggcaaa ctattatgct ggacaaacat
    12841 ttgattacgt tttgtttgcc gatgttttag aacatttggt agatcctgga aaaacgttga
    12901 agaaagttag agaatttttg aatgaagaag gagaaattct aattacattc cctaaccttg
    12961 cgcataattc tgtcatgatt gatttgttca ataatcaatt gccatgggct tcttatggtt
    13021 tgctagatga aacccataat tctttttata ctcatgatgg cttccaaaaa gtttttgaaa
    13081 aagcaggatt atacatcaat attgaagatt atttgtactt agctgtaggt gatacagaat
    13141 taaaatcaac ttatgaagaa ttgccggaag ctgtacgtta tgatttcaaa atgcgaccat
    13201 ttggcgaagt ctaccaatac ttcttctcat tgatgaaaca tccagtagca caaagttcca
    13261 ttgcgcagcc gcaaaattct aattatgtga aagtattaga agtaatccag caaacgaaac
    13321 aagatgaaat aattcaacaa ataccattca ataatttcac aggggaaaat gagacactaa
    13381 gtttccatgt atcagaatta gcagaacgaa tggtgtttcg cttcgcgcag caacctagct
    13441 ttatcgaatt cagcgcagaa gctgctggag aaaaactcac atttatcgat tcaaatgctg
    13501 tagtcaaaac agtaaatgat tgttatttgt ttgatggtaa agatttacca gagttctttt
    13561 taacggatat tgcagggaaa gaagtgacta tccattgtca ctatcgtttt atcggagaat
    13621 tgacaccaac aatgaaagaa ttgctggaaa caatccgtcc aatggctgaa gttacaaagc
    13681 agctttctga gaaaaatgat gaattagaaa gagaaaatca ttatcttatt gagaataata
    13741 caagattaga tcagacgttg aaaacaacca taaatcgcta ttgcacgttg ttggaaagtg
    13801 atgaatggac aatcaaacat agattagggc gacgtaagaa agaaacatcg aaaaaaatcc
    13861 aagaaaaaga gctttctcta tgtattgacg aaaaaatctg ggatgcagaa acaaagatat
    13921 tgaaaatcat cgggtgggga atcgctaatt cggatcgcca accattgtcc tataagttat
    13981 cagccgacca atcaccattt tttgaagcaa ttcctgtttt acgagaagaa gtcaatcaag
    14041 caaaacagct agctgaagga gcaaaagcag gatttgaact gcggatcctt tgcgagaagg
    14101 aacgttcatt cttagttgaa gcagtggctc aaaatggtca atcatggatc attgaaatgt
    14161 aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc
    14221 cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg
    14281 ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat
    14341 tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa
    14401 gacccaaagc ggttttaagg tacgtttatc agggaaaatg agaggaaaag caatcttaga
    14461 cttccaaaca gcaagacatc aaaatggcat agctgtgaaa ttaaacggta aatatcctta
    14521 tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa
    14581 ttatgcaagg actcatggcg taaaaaaagc aatccgccgg gtcaaactgg aattgaaacc
    14641 aggatcgatc gactatgcag agtggatcag tcgtcatgag aaacctgatc tgaaagagca
    14701 aaggaaagaa gtacaaggat ttgcctaccg cccattgatt tccatactta tgcctgtcta
    14761 taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtacttgatc aaacgtatga
    14821 tcactgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct
    14881 agaatcctat caagcaaaag atgatagaat taaagtcgtt ttccgacaag aaaatggaca
    14941 catcagtctg gcaactaatt ctgcattgga aatggcagaa ggagagttta tagcgctctt
    15001 agataatgat gacgaattac ctccatttgc cttatatgaa gtggcaaaag tgctaaacgt
    15061 acatccagaa ctggatttga tctacagtga cgaagataaa atcgatgcag atgggaatcg
    15121 ctttgacccg cacttcaaag cagactggtc tcccgataca ttgatgggaa ataattatat
    15181 ctctcactta ggcgtatatc ggacaagcat tgttaaggaa cttggcggtt tccgtaaagg
    15241 atatgaaggc tctcaagatt atgatttggt tcttcgtgtg acagaacaaa ttcctgcaga
    15301 acacatttac cacattgacc gtgtcttgta tcattggcgg acgatcccgg gatcaactgc
    15361 aagcaatgga gaagctaaaa gctacatcta tgattctgga gtgaaagcat tgacagatgc
    15421 tctatctaga agaaatatca agggaagcgt acatcctggc agaatttctg gtttttatga
    15481 aatcacttat gatgttttgc aagaagactt ggtcagtgtg atcattccaa ctaagaatgg
    15541 ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt atccaaatta
    15601 tgaaatcatt attgcagata atggaagtac agatcctaaa atgcaagaat tatttgcaga
    15661 atacaagcat cagctgaaag accgatttat cgtcgaatta attgacattc cattcaatta
    15721 ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc aaatactttt tattcttgaa
    15781 taacgataca gaagtgattg aacctgactg gatgacagcg atggtttctt atgcacagtt
    15841 tgatcgaatc ggctgtgtag gagcaaaatt attttatccg gatgatacaa cgcagcatgc
    15901 aggtgtatta ttagggatcg gtggcgttgc aggacatgca ttgaataact atgatcgaac
    15961 acattgtggt tactttggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc
    16021 ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat ggatttgatg aaatattaga
    16081 agtagcattc aacgatgttg acttgtgctt gaaagtttat gaactgggtc gttacaatgt
    16141 ctatgcacat caagcagaat tgtaccattt cgaatctaaa tcaagaggtt atgaagatac
    16201 accagaaaaa caaagacgtt ttgctgggga aatcaaaaaa atgcaagata agtggccttc
    16261 ttatattgct catgacccat tttataacga taatctaact aaagaaggaa tcggcgattt
    16321 ttcgttgaga cctgattgat taaaagaact gtattttcat ttttattaat gaaaatacag
    16381 ttcttttaat t
//
LOCUS       DAYYNG010000053        16375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000053 DAYYNG010000000
VERSION     DAYYNG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.053"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            503..1525
                     /gene="aroF"
                     /locus_tag="ACHBFA_001383"
     CDS             503..1525
                     /gene="aroF"
                     /locus_tag="ACHBFA_001383"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9394885.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGTQSLNEKTYSRMMKEVAIMKEAME
                     KIQRLD"
     gene            1558..2622
                     /gene="aroB"
                     /locus_tag="ACHBFA_001384"
     CDS             1558..2622
                     /gene="aroB"
                     /locus_tag="ACHBFA_001384"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9394886.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            2624..3790
                     /gene="aroC"
                     /locus_tag="ACHBFA_001385"
     CDS             2624..3790
                     /gene="aroC"
                     /locus_tag="ACHBFA_001385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9394887.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRAAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSQVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSIDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            3807..4898
                     /locus_tag="ACHBFA_001386"
     CDS             3807..4898
                     /locus_tag="ACHBFA_001386"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9394888.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERTNLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            4911..6206
                     /gene="aroA"
                     /locus_tag="ACHBFA_001387"
     CDS             4911..6206
                     /gene="aroA"
                     /locus_tag="ACHBFA_001387"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9394889.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     THTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLQGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            6199..6708
                     /locus_tag="ACHBFA_001388"
     CDS             6199..6708
                     /locus_tag="ACHBFA_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9394890.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQRLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            6705..7541
                     /gene="pheA"
                     /locus_tag="ACHBFA_001389"
     CDS             6705..7541
                     /gene="pheA"
                     /locus_tag="ACHBFA_001389"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9394891.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPACLRALFRKQV
                     DLAVVPVENSLEGSVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIIGDRKITSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7604..9340)
                     /locus_tag="ACHBFA_001390"
     CDS             complement(7604..9340)
                     /locus_tag="ACHBFA_001390"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9394892.1"
                     /translation="MRKKIKEHELFTKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVESYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9337..10266)
                     /locus_tag="ACHBFA_001391"
     CDS             complement(9337..10266)
                     /locus_tag="ACHBFA_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394893.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNLDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFLK"
     gene            complement(10266..10845)
                     /locus_tag="ACHBFA_001392"
                     /pseudo
     CDS             complement(10266..10845)
                     /locus_tag="ACHBFA_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            11118..12404
                     /locus_tag="ACHBFA_001393"
     CDS             11118..12404
                     /locus_tag="ACHBFA_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9394894.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADNEEKVTVWAWDET
                     FNTKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFADLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMDNLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQVNASLKYGLELFATILKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            12541..13410
                     /locus_tag="ACHBFA_001394"
     CDS             12541..13410
                     /locus_tag="ACHBFA_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394895.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQWMLTDATFKKAFFNTFLYLLVQVPIMLFLALFICNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWAKVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLRLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            13407..14293
                     /locus_tag="ACHBFA_001395"
                     /pseudo
     CDS             13407..14293
                     /locus_tag="ACHBFA_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            14461..15144
                     /locus_tag="ACHBFA_001396"
     CDS             14461..15144
                     /locus_tag="ACHBFA_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9394896.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            15141..15914
                     /locus_tag="ACHBFA_001397"
     CDS             15141..15914
                     /locus_tag="ACHBFA_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9394897.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGFPYNYILVPLGFLMGFLVGF
                     AEPAIHVMVKQVEELSEGRIRSKVMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            15911..16309
                     /locus_tag="ACHBFA_001398"
     CDS             15911..16309
                     /locus_tag="ACHBFA_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM9394898.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENKDREKSES"
BASE COUNT         5120 a         2902 c         3631 g         4722 t
ORIGIN      
        1 aactatcaga aaattaaaaa aagtttcata aaaatgattg acagtcgatt tttttatgtc
       61 tatactaggt acaattctta catcagtttg gagcgatttt ttatgaaatc tagaaaactt
      121 acaaaactga atacgtatta ctttagctat tttagatagg tttgtattca tgacttcgtg
      181 ggtacaaaca aaccaggttt gtatctatga tggctaaaga tttttaaaca cgacttacag
      241 ccacgtagag aaattcatct atagtggcta ttttatattt ttgcagcgct ttttaaggct
      301 cgttgaagat gttcactgta gaggcaattt cctacaggga catctttttt tgttgattat
      361 ctgggtcata agtaggaaag aaggagcgaa aatgagaaca acaggacacg gtcatttagt
      421 aggaattaat gtaaaaccac cttgaaaaag tagagaaaat cagccaagat agataagtaa
      481 acaaaagaaa gcgggaaaaa caatgatagt aattatgaaa aaagaagcaa cggaagaaca
      541 aataaaaaaa gtcgtcgatc atatcgaaaa agaaggattc aaagtctcaa tcgatcaagg
      601 aacagaacga gtagtgattg gactaaaagg tgatacgcga agtttacaag aaggagcatt
      661 tacacgctat gaaggagtag aaaatgctgt gcgtatttta aacacataca aattgacttc
      721 tcgagaattc catcctggca atacagtcgt ggatgtagac ggagtaaaaa ttggtgacgg
      781 cagtttcgtg acgatggctg gtccttgttc tatcgaagga ttagaccaaa tcagagaatg
      841 tgcgcgaatg gccaaagccg ggggcgcaaa aattctgcga ggtggtgcct tcaaaccacg
      901 tacttctcca tatgctttcc aaggtttaga agaagaagga ttgaaataca tccgtcaagc
      961 ggctgatgaa tttggaatga aagtgattac tgaagtgatg gatgaagggc atatcgatat
     1021 ggtggctgag tacagcgata tcctacagat tggcgcacgt aatatgcaaa attttaaact
     1081 gctatctgct gtcggtaaaa caggtaaacc tgttggattg aaacgtggaa tttccggtac
     1141 gatcaatgaa tggcttaatg ctgcagaata cattgcagtc gaagacaaat ctcctgtcat
     1201 ttttatcgaa cgtgggattc ggacttacga aacagcgaca cgtaatacgt ttgatttaag
     1261 tgctgtccca ttgatgaaaa aattaactca tttccctgtg attgttgatc caagtcatgg
     1321 tacaggaatt tgggaacttg taccaccaat ggctcgtgcg ggtgtggcaa gcggagcaga
     1381 tggcatgatc gtagagatcc accctgatcc tgcaaatgct tggtcagacg gtacccaatc
     1441 gttgaatgaa aagacttact cacgcatgat gaaagaagtt gcgatcatga aagaagcaat
     1501 ggaaaagatt cagagactcg attaattcca gctttttaaa agaaagaagt gaaacgaatg
     1561 cttactgtac atgttgataa ccaaaaatac gatattcata ttgaaaaagg cgcactagaa
     1621 caggctggat catggcttca aacgatttgg agacctcaaa aagtagctgt gatcactgat
     1681 acaaacgttg ctccactata tgcagaaaaa atcatgttcc aattgagaca agctaatttt
     1741 gaaccagtcc tttcaatcgt accggcgggt gagaccagta aatctttaga acaagcagct
     1801 gagttatacg attttttagc agataatgat tttacccgca gcgatgggat acttgcactt
     1861 ggtggaggaa tggtgggtga tctagccgca ttcgtggcct cgacgttcat gcgtgggatc
     1921 catttcgttc aaattccgac tacgctactt gcacaagtag attctagtat cggcgggaag
     1981 acagcagtca atacaaacaa agcgaaaaac cttgtcggaa catttagcca gccagctggt
     2041 gtattgattg atcctgaggt tctaaaaact ttgccggtac gtcgtttacg agaaggaatc
     2101 gctgaaatta tcaaatcagc agctatagca gatgcaacgc tttggaaaca gctgaacgag
     2161 ctgaaagacg aaaaagatct tctggaacat gctgttccaa tcattactgc tacgttggaa
     2221 atcaaacgaa aagtagtgga agaagatgtc tttgaccaag gaaatcgact tattttaaat
     2281 tttggtcata caattggcca tgcaattgaa aatacagcag gatatggggt agtcagtcat
     2341 ggagaaggag tagctattgg aatggtagcc atcacttctc atgcagaaaa aatcggcttg
     2401 tctccaactg gatcgacagc tgctttagaa aggatacttc aaaaattcca tctgccgatt
     2461 acgtacgatc taagtcatcc agatgaatta ttccacgcga taacccatga taagaaagca
     2521 agaggaagcc aattgaatat catcttgcta gaaaagattg ggcaagcaaa aatcgttcct
     2581 gttcccacag agacattcaa agattactta ggaggactgt aagatgcgtt atatgactgc
     2641 gggagaatcc catggaccag aattaacagc gattattgaa ggactaccag caggattacc
     2701 tctgtcagaa gaagaaatca atcacgaact cgctcgcaga caagctggtt acggcagagg
     2761 aggaaggatg aagatcgaaa aagatcgtgt tcggatcacg tctggcatcc gtcacggcaa
     2821 aacaattggt tcacctgtca ctttagtggt agaaaataaa gattggaaaa attggacaac
     2881 tgtcatgtct gtagcagaag tgcctgaaaa agaccaatca ctgcggcgag ttgcacgtcc
     2941 gcgtccagga cacgcggatt tggtaggcgg tatgaaatac catcatcgag atcttcggaa
     3001 tgtgcttgaa cggtcctctg caagagagac gaccatgcgt gcagcaatcg gcgcaattgc
     3061 caagaaaatt ctggaagaac ttgacatcat cgtagcagga cacgtgattt gtcttggtgg
     3121 aatccaagca gaaatacctg gaaatatctc gattcaagaa atcaaagaaa tttctgaagc
     3181 gtcagaagta cgagttgttg atccagcagt tgagcaaaga ataaaagatt tgatcgatca
     3241 gacaaagaaa aatggagata cgataggagg aattgtagaa gtgctcgttg gaggtgttcc
     3301 agctggatta ggaagctacg tacaatggga cacgaagcta gatgccaaaa tcgcacaagc
     3361 tgtggttagt atcaatgctt ttaaaggcgt agaatttggg gtcggattca cttctggttc
     3421 attaccaggc tcacaagtga tggatgagat cctatgggaa gaagaaagag gctatttccg
     3481 acgtaccaat aatctaggcg gcttcgaagg cggaatgacc aatggcgaac cacttgtcat
     3541 tcgcggggtc atgaaaccca tccctacatt gtacaagcca cttcaaagta ttgatatcga
     3601 tacaaaagaa ccttacaaag caagtgtaga acgttcggat agtacagcag ttcctgcagc
     3661 tagcgtcgtt gcggaaaatg ttgtagccac tgtggtagcg acagagctat tgtcaaaatt
     3721 cccaagtgat tcgcttgttg agttgaaagt tgcagtgaag gagtaccgtg actacttgaa
     3781 acattattaa aaagggggca aataagatgg cacaacatgt ttttatcgtg gggcttggac
     3841 tgattggtgc ttctatggca ctttgtattc gtcagaccaa tccagaactg atagtagttg
     3901 gttgggatag tcagtcaaaa acccgtgagc aagcagtaga acagcagatc gttgattacg
     3961 ttgcaagtga ttttgaaaca ggtgcagaac aagcagacgt tatcttatta gccgttcccg
     4021 tccgtacaac cttagcttat cttgatgtcc ttgaaagaac caacttgtct gatcacgtca
     4081 tcattacgga tgtcagcagt acgaagcagc aagtcgtgac atccgctgag cagaaaaatc
     4141 ttcggtttgt cggtggacat ccaatggctg gttctcataa atcaggtata caggcagcag
     4201 atgctaaact atttgaaaat gcctattaca tctttacacc tttggaaaag acgaaaaaag
     4261 atgttgaaaa actgcaagaa ctattttgcg gaaccaaagc aaaatatgta actcttacag
     4321 caagagaaca tgaccgtata acaggaatgc taagtcattt tccacatatt ctggcagctg
     4381 gcttagtcaa tcaggcagaa caattcaatc aagagtatcc gcgagccaaa caactggcag
     4441 caggaggctt tcgagatatc actaggatcg cttcctcgga tcctgttatg tggacagata
     4501 ttttgttgag caataagcag atattattgg aacgattggc ggattggcag caagaaatgg
     4561 cacaaatagc agaatggatc atgactgaga accaatcgga aatcttttct tttttcaatc
     4621 gggcaaaaga aagtcgagac cagttgccta tccataagca aggcgcgatt cctgcctttt
     4681 ttgatttatt tatcgatgtg ccagatgaac ctggcgtgat tgcagaagta actggactga
     4741 tcggaaaagc gggagtttct ttgattaatt tgaagatcca agagaccagg gaagatattt
     4801 taggaatact gcaaatcagt tttaaaaacc aacaggattt gttacaagca aaaagaatca
     4861 ttatgtctaa taccagttac cattgctgga tcaaatagga ggctggataa atgcaattat
     4921 tacaacaaat acatggatta agaggaactg ttaggatacc agcagacaaa tcaatttctc
     4981 atcgcagcat catgtttggg gcaattgcgg aaggaacgac gactatacaa aattttttgc
     5041 gcgcagaaga ttgtctgagt actttacatg ccttccagca attaggcgtt gaaatcgaag
     5101 aagaggaaga gatgatcaag attcatggtc gcggtagcca ctcatttgtc caaccaaccg
     5161 aatcaatcga catgggaaac tccggtacga ccatacgttt attgatgggt attttggctg
     5221 gacagccttt tacgactact ctgttcgggg atgcttcgtt gtctaaacgt ccaatggggc
     5281 gagtgatgga acctttacgc gagatgggcg ctgacttgca aggaaaagag aatgagcagt
     5341 atctaccaat cactgtgaca ggaaccaact ctttatcacc tatccgatac aatatgcctg
     5401 tagccagtgc gcaagtcaaa tctgctttgc tgtttgcggc attacaagca gaaggcacat
     5461 ccgtaatcgt tgaaaaagaa cgttcccgta accatacgga agaaatgatt cgtcaatttg
     5521 gcggaaggat ctcagtagaa ggtaaaacaa tcacggtaac aggaccacaa aaattaactg
     5581 gtcaacagat aactgttcca ggcgatattt catcggctgc tttctttcta gcagcagggc
     5641 ttcttgttcc ggaaagccaa ctgttattaa aaaatgtcgg ggtcaatcca acaagaaccg
     5701 gtatcttaga tgtgctggag gagatgggcg cagcgattac ccatacgaat cacaatgaac
     5761 agaaccaatc ggctgattta agtgtcaaga cgagtcattt gaagaaagca acgattggtg
     5821 gagaaatcat tccacgtctg attgatgaat tgcccattct agctttagtg gctactcaag
     5881 cagaaggaat cacgatcatc aaagacgcag aagaattaaa agtaaaagaa accaacagaa
     5941 ttgatgcggt ggcagaagaa ttacagaaaa tgggagcaaa gataaaagcg acagcagacg
     6001 gtctgatcat ccatgggccg acacctctgc agggcgcaaa agtgtccagc agaggcgatc
     6061 atcgaatcgg tatgatgctg caagtagctg ctttgatagc tgatggacct tgcgaactag
     6121 aaggagctga agctgtttca atctcttatc cggcattttt tgacgattta gcagaacttg
     6181 tgtcaggagg agaagctcat ggctagcatt ttgctgattg gtttcatggg cgctggaaaa
     6241 acaacaatcg gtaaagggtt agctcaacgt ttacaaaaag agtatgtaga tttagataca
     6301 aagattgagg aacatatcca attgtcgatt gccgaatatt ttcactatta cggtgaaaaa
     6361 agctttcgaa aagtggagtc agatatttta agaaagctgt caaatgaaga taaaattatc
     6421 gcgactggtg gagggattgt ccaaagtgca gaaaatcggc gttttttaaa aacacagccg
     6481 attgttcttt acttagaggc agaagcggac tgtctagttg atcgtatcca acaagatgaa
     6541 acgtctattc gacctttagc tcttggaaaa acaagagaag aaataaaagc attattggca
     6601 caacgacttt cttggtatga agaaagtgcg acacatcgaa tcaacacgac caatcaaaca
     6661 ccagaaaaga tcatcgatac cattatagaa aggatcaaaa gcatatgaac gtcagctatc
     6721 tagggccaga aagttcattc acttatcagg cggcttgtca gctatttcct acagaacaat
     6781 tgactgcgta tgcttccatc cctgcttgtt tgcgcgcttt gtttcgcaaa caagtcgatc
     6841 tggctgtcgt tcctgtagag aattcattgg aaggatctgt tcatcacaca atcgatcttc
     6901 tttccaaaca tccagaagtc gaagttaaaa gtgaaatcgt tcttcctatc aagcagcagc
     6961 ttttaggcaa tccagcaaca aagattacaa aaatcctttc acatccacaa gcactagcac
     7021 aatcacaaca gtttttagaa acgcattatc ctaatgttcc tttagtagca acagaatcaa
     7081 caactgctgc agcaatgtat gtagcagaac atccgaaaga agacgcagca gcgattgctt
     7141 cattagaaac agcacagcat gttggattgg agatcttagc tgagaatatc caagataatg
     7201 agttgaatca gacacgtttt tggatcatag gtgaccgaaa gatcaccagc cagcagtcag
     7261 ctccagtaaa aatgagtgtc atcttgacac tacctgctaa tcggccaggg atgcttcata
     7321 aaatgctggc agcttttggc tggcgggaaa tcaacttaag taagattgaa tcccgaccat
     7381 tgaaaactag cttgggagaa tattttttcg tgatcgattt gttgttggac cgcccaatga
     7441 cattagtaga gaatgcgctc caagaaatca agatgctcgg aggagaaagt caaatattgg
     7501 gctgttatcc agtgctgaca gtagaagaga cgcgtagatg aaagtcaaga atactgattg
     7561 atttatttaa cttaaaataa aaaaatcagc gcttggctat tctttatttt tgatattctg
     7621 attgattttt aggaagaagc ggtagacaaa tcgttacagt cgttcctgtc ccaatcactg
     7681 attggatcgt taagccatat gcatcgccat acattaggcg tatccgctca tctacatggc
     7741 tgatgccgat tcctgaaaaa tgatcatttt tttgtttggt attttcagta tcgaagccat
     7801 cgccgttatc aatcacttcg aataaaagaa gctgattttt tgttttgccg tacaccgtga
     7861 tgaatccttg cgttttattg tgaaaagcat gaagataggc gttttcgata attggttgaa
     7921 ggatcatatt cggcatagat aatgactgca gttcatcagg aatcattatt ttagccgaga
     7981 ttctttcgcc atatcgcata ctcaaaagat cgatgtaact ttgtacgctt ttgatttctt
     8041 ccttgactgg aatcacttct tcttttttcc caatcgtact tcttagtaaa gtaatgaaac
     8101 tgtcgattcc ttgaactgct tcatctgttt ttttctgcca aatcaaaaat ttcaagctag
     8161 tcagcgtatt ataaataaaa tggggctgta tctgtgcttg catagtttgg atttcaagcg
     8221 aacgtttctt cttttcgtct tggatcgtct gttcataatg atgctgagca ttacttagca
     8281 attgattgat cgtattccct agcaattgga cttcatggct tccttgtaca ggaacagtag
     8341 cttttaagcc atcttcctct agccgtttta gcgaatcaat caagcggtag atcgattttg
     8401 tgtgtcgatc caaatacata aataccaatg agccagcaaa agcaagtgtc acgatattat
     8461 acaaaataat cggaaaaatc acgcccattt ggttagacaa agaatgaaga tcgacttgac
     8521 tgagtaatgt acaatcctgg cggtacaaag gaagacgggt caactgctgt ccatttgaaa
     8581 tcgttgtttc acctactgtt agttttttga aaatcattga tcggctccca attgtatttt
     8641 cttcatttga tgcgatgatc gtctgttctt cgttcatgaa ggagatcttc gatatctcag
     8701 gatttactac agattgaaaa agattcgcta gttgcttgga tgagatcgat aaaacagcat
     8761 atccgtaaac gtgattcaat gtatttttca gttttttgac aaagaagacg ctgggtgctt
     8821 tcttatcgcg aagagtcaat ccgtggtgtg caaaaagcat ttgtgtatca gcagcacgtt
     8881 cattcactct ctgagcaatc gcagattgaa aaaaagaatc ggcagatacg gcagtgagca
     8941 tgtcgtttcc gacaaaagtt cttccatttt ctccaaagat caatacatcg taatcgatca
     9001 ttggatggat ttttgaaaga gagtcattca ttttttggat tgttcttagt tgctgaagct
     9061 gttccatatg ttgggctgga tcattggaaa aatagctttc gacagctgga ttagaatcaa
     9121 aagcggctaa tgtattctcg attgtatcat tcaagctttc aaattcagat tggatctttt
     9181 ttagtaccac atcattcgat tgattgaaca gctggatata ggcatccctt gatcgatgat
     9241 aaataaacaa agagatgacg atggcttgca gaaaaagacc gatgaacaaa atgaacagaa
     9301 gtttggtaaa gagttcatgc tctttgattt tttttctcat tttaaaaaag ttcctttacg
     9361 gtattgagaa ggtgtcattc ctgtctcttc tttaaaaact ctggagaaat aacccagttc
     9421 ggtatagccg actttctcac agatttccgt caaagtaagc cgtgtttccg tcaaaaagag
     9481 cttggcttta ctgatacgga ctttttttag atattttgaa aagttgatac caaatttttc
     9541 agtaaagatg gctgagagat agctatatga aaaatgatat ttttcggata aaagttgtaa
     9601 attgatttct ttctcataat tttcctggac gaactcgtag atcaaagtca agatatctaa
     9661 attgtctttt tttgcatttg cagaaaagtg ggtgagtttc tcaagtgttt ctttgacaag
     9721 aatagaaaaa tcttcgaaat actcagcatc ggctaataaa gaaggaaggg cgatcttgag
     9781 caaagaaagt tcttcattcc caacatgctc gtcagccaaa gcgttgagta atgcatagaa
     9841 acgattgatt gcctcatgtt tcaaataagc tggatcaata ggctgttcca atatcgtcgt
     9901 gaagaaattc tcaagttctt tgacagctaa agcgtagtct ttatgaacga tttttgttaa
     9961 ataactactt gcttgctgct cgtggttggt taataacgga agaagttcat gctgataagc
    10021 aaggttcttt cccttatgat agaaacgttg tcctttggtg caatttctga tctcttggaa
    10081 tatttcattg atatcttgga ttgcaaaaga atcagaaaca acaaaaaaat cacctggaaa
    10141 aacttcttga cgaaactttt gaacaaactt cctaagtgta tctttggaat cagtattgat
    10201 tacatagccg gattcttccg aaaaggtaga aacaggataa atagtaaacg aatcatcgct
    10261 atccatcaat gaatgggtga tcgactattt ttgtttagag ccttgttttg aactgaatgg
    10321 ccgtttttcg gtatataaca gaaaaaacag ggtgtgttca aaaagaagag ggtcaagcaa
    10381 tggacgagtt tgtccagcaa tctgctgtga aagattctcg cagatctgct cataacgatt
    10441 ggtgattggg cttttgttgg gctttttttt gattttttgt gaaacggcag ttaaggaatt
    10501 tagaaaaaaa tcaggactca atcgaggctt taaaagataa tcagcgacgc cttctttaaa
    10561 agaatcttta accatatcaa attcatcata gctagagagg ataaggatct gtgtttcagg
    10621 ttgttgctgt tttaatagcc gcgataattc gattccattt aattcaggca tcattacatc
    10681 tgtgaccacg atatcgattg catgtaccaa agaaaaatca agcgctttca atgggctgga
    10741 aaaagtccct attaattgaa acccgtgctc agaccaattg atcaactgtt tcattccatc
    10801 taaaatcgtc tgttcatcat cgataagaat tacgcgtatt ggcatttttt tacccgcctt
    10861 aagaatagtc ttataattta aaaatactta tttctaggag aaaatgcaag gatattttta
    10921 ttttaaagaa acctcaccat ttcgatcttt ggtctagatg ctaagattat tccttttgtc
    10981 atagttgatt tgtatttttt tattatttta ggagaaattt ttgaagtatc tgaataataa
    11041 agaaaccgtt ttcaaatttt ggtatgatga atgtagcaaa cgtaaacaac taatggatgg
    11101 aaaaggagcg gataaaaatg aacatcagaa agttaattgc tggagtcgtt tgtttagcgg
    11161 cagcatcagg tttcttaaca ggatgcggaa gtgctagtag tacagcagac aacgaagaaa
    11221 aagtcactgt ctgggcgtgg gatgagacat tcaataccaa agcagtgaat gaagcaaaaa
    11281 aagtttatga gaacgaagaa acagagatag aagttgttac gatgtctcag gatgatatcg
    11341 ttcagaaact gaacaccgct ttagcgagcg ggaatacaga aggattgccc aatatcgtct
    11401 tgattgaaga ttataggata caaggctatt tgacttctta tccagatgca ttcgctgatt
    11461 tgtctgctat tgtaaaggaa gatcattttg caccgtataa atttgcggta aacaaagtag
    11521 gagataaaat atacggtgtt cctttcgatt cgggcgtaac agcaaacttc tatcgtacgg
    11581 atctgatggc agaagcaggt tatacagaag aggatatgga taatctaaaa tgggacgagt
    11641 atattcaagt agcgcgagat gtcaaagaaa agacgggaaa gaaaatcgct gaagtcaatc
    11701 cttctgacct tggtcgagta aggatgatta tgcaacaggc aggagagtgg tatacatctg
    11761 aagatggaga gacagtcacg atccaagtca atgcatcttt aaagtatggt cttgaattgt
    11821 ttgccactat tttgaaagaa gatctagtcg aacaaacctc tgactggaat gctggagtaa
    11881 ctgcgataca atcaggagca gtagcttcta gcccaactgg cgcttggtat tcttcaacga
    11941 tccaaggagc agaaaaccaa tctggaaaat ggaagattgc accaatacct gcacttccag
    12001 aaaatctgca aaaagcgcaa gcttctaacc ttggcggcgc tggctggtat gtgatcaaag
    12061 gagtagctgg agaagattca gcgaaagatt tcctagaagc aacgtttgct tcgaatgaag
    12121 aactgatggg cactcttgcg aaagaaatcg gactagtatc tacgatgctt agcgcgaatg
    12181 aacaagaggc ttatcaagaa gcttctgaat tttacagtgg tcaaaagatc tttgacgatt
    12241 tttcaacatg gacatctgaa ataccagaag tgaattacgg acatgagacc tatgcaattg
    12301 aagcagtcgt agctgaagct cttcatcgaa tcatcaatgg agaagagaca gacaaggtac
    12361 tagcagatac acaaaaacaa gtagaagcac aattggctaa ttgatcggtg gtaaaaaagt
    12421 gaatttcttc gctttttcat cagacaaacg aaaagacaaa aaagatcacg tctgccttac
    12481 agatgttgtc tttcttgtct tttcatgtag ataatcaatg atagaattgg agggaaaaga
    12541 atgaagcaga aagatttatt caaaaacggg catgcttttt taatcttgcc agtcattttg
    12601 atatcgatca tggtttttat tccgatggtt ttggctttga tcacgtcttt tcaaagtggt
    12661 ccccctgtca atatgacatt taacggatta ggtaattatc agtggatgct gacagatgct
    12721 acttttaaaa aagctttttt caatacgttc ctgtatttac tagttcaagt accaatcatg
    12781 ctgtttctag cgctttttat ttgcaacctt ttaaatgata aaaagctgaa atataaaggg
    12841 ttgtttcgaa cagctatttt tcttccgtgt atcacttcga tggtcagtta ttctctgatc
    12901 atgaaaagtc tattttctca atctggcctt gtaaacaact tgttgttggg cattggtatg
    12961 attgatacac cgattggctg gctgactcat ccaatttggg cgaaagttct gatcattctt
    13021 tcgatcactt ggcgttggac tggatacaat atgatttttt tcctttcagg catgcaaaat
    13081 attgattcgg agatttatga agcagcatct attgatggtg cttctaaatg gcagcaattt
    13141 atcaaagtga cgatcccgaa tttaaaaccg attattttgt ttacttccat tacttcgacg
    13201 atcgggacat tgcggctttt tgatgaagta caaaatatta ctggcggggg tccagcaaat
    13261 gcaacgacca ccctgtctca atacatctat aatttgagtt ataaattcac gcctaatttt
    13321 ggatatgcgg cagcagtttc ctttgttatt gtttccttta ttgtgatatt gtcgataatc
    13381 caaacgaaag taggaggaga aagtcgatga aaaatatggt agaaaaagaa acaatcgaac
    13441 gccccaacgt caaaaaacaa gcattagaac ttcgttcgac aattgggtca gtattaaagt
    13501 atacagcatt aagcatccta tcatttattt ccattttccc ttttgtctgg atggttttgg
    13561 gaatgacgaa tacacccgtg gatatcacat cgggaaaaat aaagattggc agtaacttga
    13621 tcatcaattt tcaaaatttg ttttccaatg agctgaattt tactcgttcc ctttggaatt
    13681 cagcgatgat cgctttggtg accactgtgt ttgcattgct gatttcgtca atggcaggat
    13741 atggatttga gatttatcaa agtaaacgaa aagacctaat tttcaatatt ctactgctat
    13801 cgatgatggt tccttttgcg gcattgatga tccctttgta tcgaatgttt tcacaattga
    13861 acggaacggt tttgggaatc aacagtttct tggttgtcat cctgccatct gttagcactg
    13921 cttttttgat cttcttcttt cgacaaaatg ttaaatcttt tccaaaagat ctagtagaat
    13981 ccgctcgatt agacggtcta aaagaactaa gtattttttt agagtgtata tgccgacggc
    14041 taaaaatacc tatgccgcgg ctgcaatcat cacattcatg agtagctgga acaattattt
    14101 atggccctta gtagctttgc aatctcctga taaacggacg gttccgcttg ttttatctgc
    14161 tatgggtgct tcctatacac cagactatgg aatgatcatg gctggattag tcatcgctac
    14221 tttaccaact gccatcgtat tttttgtttt gcaaaagcaa ttcgttcaag gaatgatcgg
    14281 ctctgtaaaa taaaaaaaac acaataagcg cgttccttca aagtcatttt tccaagtaaa
    14341 aagatacttt tgggaagcgt gttattttat gttgagaaaa tttaaatgat aacgttttaa
    14401 gtttgtgctc gaaaactctt tcattttaag gtaaacttaa agagtaaagg aggaggatac
    14461 atgctaaaaa attttaaaga agtgattgct gctatattgc cgatgactgt cctgattgtc
    14521 attttaacgt ttgtttttgc accgttagaa ggagaagacc tcgcttcatt tctggttggt
    14581 gcggcaatca tgatgatcgg aatgacgctc tttttgtttg gagcagatta ttctatgatg
    14641 gaagtcggag atctagtagg gaaatatatg atcaaaaaga aaagtttgac tgttttggtc
    14701 agtttgggat ttgctatcgg gatcgtgatc acgattgctg aaccttccgt gcaagttttg
    14761 ggacaacaag tatatgatat atctgacgga gagattggca gagtgttgct gattgggatc
    14821 gtcagcgtcg gcactggagt atttttagcc tttgcgctac ttcgggtcgt attcaagcta
    14881 tcctattatc aattgatggc tataggctac ataggcgttt tgatcgcctc atttttcacc
    14941 agttccgaat ttatgccgat tgcttttgat tcgggcggag taactactgg tccagtaact
    15001 gttccgttta ttttggccct tgccggaggg atgactagca tgatcaaaca aaagaaaaat
    15061 gaaaatgaca gttttggaat ggtcggtatt gcttcgttag ggccgattct tgccgtgatg
    15121 attttggggg tgatcttcca atgagtttct ggcagcaagt gatagatgga atcgaaatcg
    15181 ttaccatgga agtcattttg gcagtggcac ctatcgtaat cctatttgtt ttaatgaata
    15241 tcattagttt tcgtatgcgc aaaaagcgtt tctttgacat tatgaagggt ttcttgataa
    15301 cgactattgg gttgatcctt ttcctgcacg gagtcaatat ggcttatgta ccggtaggac
    15361 agcatttagg cagtgctatt gcgggatttc cttacaatta tatactggtt ccccttggct
    15421 ttttgatggg gttcctagta gggtttgctg agccggcgat tcatgtaatg gtcaaacagg
    15481 ttgaagaatt gagtgaagga aggatccggt cgaaagtcat gctgatggtg atttcgattg
    15541 ggattggcgc agcagtaggg ttgtctatgt ggcgtttgct cgtcggattc tctctttatt
    15601 atttcttgat cccaggatat gggctagcat ttattttagg acgaaaagtg gacaagatct
    15661 ttttagcaat ggcttttgac aacggtggag tagcaacggg tccgatgtgt tcgacattta
    15721 ttttatcaat gagcgtggcg atcgctgcac aaattgaggg aagagatccg atcattgacg
    15781 ggtttggtgt ggttgcaatg attgcattga caccaatatt atccacttta gtactaggat
    15841 atatttataa acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg caaaaattgg
    15901 gggaaagcaa atgaaagatc ttgtagcatt gaatcttcac atgattgtga cgatcgttga
    15961 cggaggaatg ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag caggcggaac
    16021 gatcttacat ggaagaggtt ctggtgtaca tgattcaggg aaatttttag gtttggacat
    16081 ttcgccagaa aaagatattg tgttgacatt agtacccgat agtttgacga ataaagtaat
    16141 ggatgcgatt ggtcaaggca tcaacatcga tattccaggt aatggtattt gttttagtat
    16201 tgatgtagac aaagtcattg gtatcactaa gattcatcaa tatcgagaaa ttatcggaat
    16261 gcaagaactt ttagatgaaa ataaagatag agaaaagtct gaatcataga caagcgtttg
    16321 aagaggaaat aaacataaac aagaaggcgg atgagcgtta tttccgcttt tttgt
//
LOCUS       DAYYNG010000054        16338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000054 DAYYNG010000000
VERSION     DAYYNG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.054"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..531)
                     /locus_tag="ACHBFA_001399"
     CDS             complement(34..531)
                     /locus_tag="ACHBFA_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9394899.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(661..1371)
                     /gene="budA"
                     /locus_tag="ACHBFA_001400"
     CDS             complement(661..1371)
                     /gene="budA"
                     /locus_tag="ACHBFA_001400"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9394900.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1384..3048)
                     /gene="alsS"
                     /locus_tag="ACHBFA_001401"
     CDS             complement(1384..3048)
                     /gene="alsS"
                     /locus_tag="ACHBFA_001401"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM9394901.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3253..3915)
                     /locus_tag="ACHBFA_001402"
     CDS             complement(3253..3915)
                     /locus_tag="ACHBFA_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9394902.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3925..4740)
                     /locus_tag="ACHBFA_001403"
     CDS             complement(3925..4740)
                     /locus_tag="ACHBFA_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394903.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5002..5445)
                     /locus_tag="ACHBFA_001404"
     CDS             complement(5002..5445)
                     /locus_tag="ACHBFA_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM9394904.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5579..5917)
                     /locus_tag="ACHBFA_001405"
     CDS             complement(5579..5917)
                     /locus_tag="ACHBFA_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9394905.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5905..6282)
                     /locus_tag="ACHBFA_001406"
     CDS             complement(5905..6282)
                     /locus_tag="ACHBFA_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9394906.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6506..7699
                     /locus_tag="ACHBFA_001407"
     CDS             6506..7699
                     /locus_tag="ACHBFA_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9394907.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7865..8287)
                     /locus_tag="ACHBFA_001408"
     CDS             complement(7865..8287)
                     /locus_tag="ACHBFA_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9394908.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8682..8876
                     /locus_tag="ACHBFA_001409"
     CDS             8682..8876
                     /locus_tag="ACHBFA_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394909.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8873..9367
                     /locus_tag="ACHBFA_001410"
     CDS             8873..9367
                     /locus_tag="ACHBFA_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394910.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9529..10701)
                     /locus_tag="ACHBFA_001411"
     CDS             complement(9529..10701)
                     /locus_tag="ACHBFA_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9394911.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10907..11722)
                     /locus_tag="ACHBFA_001412"
     CDS             complement(10907..11722)
                     /locus_tag="ACHBFA_001412"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9394912.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11872..12318)
                     /locus_tag="ACHBFA_001413"
     CDS             complement(11872..12318)
                     /locus_tag="ACHBFA_001413"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9394913.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12315..12830)
                     /locus_tag="ACHBFA_001414"
     CDS             complement(12315..12830)
                     /locus_tag="ACHBFA_001414"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM9394914.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(12903..13817)
                     /locus_tag="ACHBFA_001415"
                     /pseudo
     CDS             complement(12903..13817)
                     /locus_tag="ACHBFA_001415"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(13814..16141)
                     /locus_tag="ACHBFA_001416"
     CDS             complement(13814..16141)
                     /locus_tag="ACHBFA_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM9394915.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4673 a         3253 c         2884 g         5528 t
ORIGIN      
        1 gatttctctt cttttttgtt tcaatagacg attttagtta ttaataaatg aaaaagcatt
       61 tatattaaaa tttgctaata aaagatctac atatttttct agaaatgctt gatcgatttc
      121 atcataccct ttcgttactc cgcaatcgat atctaaaaca ccgattaaat taccttcttt
      181 taccattggc actacgattt ctgattgtgc ggctgaatcg caggaaatgt aatttgcatg
      241 ggttttgaca tcatcaacga tcaatgtttc ttgtttttca gctgattctc cacaaactcc
      301 ttttcccatc ttgatacgtg tgcaagaaac tcgcccttgg aacggtccta aaatcaattc
      361 attaccatcg aataaataat atcctgcaaa gacagtctca ggcaaagtct cattcaacaa
      421 tgcagatgaa tttgctaaat ttgctaacca gttatcttct gcttctaata atccttgttg
      481 ctgtaataat aaaatttcgt atgcatcttt tttctgtttt tctgtccaca tgataaaaac
      541 cgctcctttt tgatctatgg agaagattat agctcagttt ttttgtaaac gctaggtttc
      601 ctcctcaaaa aaacgagata cgtcaggtta atcaccttga acgcatctcg ctgtcttttt
      661 ctattctgct tgttggattt ctgctgctaa atcagcataa tcaatcgttt cgtgaacaaa
      721 agtcggatca tcaactggaa aatgctgtac aagggacgag atgcggctaa tctcaacttt
      781 tccttcagcc acttcaaaat ccattacatg tccgccaaat gtatgtgtat catcgataaa
      841 atgcaagtga aatcccgctg cagcaactcc ttggaataat tcaggcgtaa agaacccgac
      901 taatgttcct tggacattgc tttgttggaa ttctggttga gactctgaaa tttttgctag
      961 tcttctgtaa ggtttttctt gcttcggcat gatacggatg tgcatatttt tgaataatcc
     1021 agttatcttc actgccaaaa ataggttttc cccggcttct cttactaata tgtctttttt
     1081 caattgttct ccatctgcag tttttgagac agaaaaacta gtatctggcg taaaatctgt
     1141 aattgctgca tagggggtaa gttcacttcc atctaattgg gcaactgagc catctgatct
     1201 tccttgataa gctacgccat ctaaaaagat gacttcacca tcaagcccat gcaatgttcc
     1261 caatcccaga gttccttttt ctaatatatg agcaatcgtt tctgttccat ccatcaagcc
     1321 agccatcaaa gctcctaatg tcccgtgctg gtataagatt ttttcagtca tgtcgacact
     1381 tccttaatag aattgatctg gtaagattgt ttctcccaat ttctcattat cacggtaatc
     1441 aatcggaata tcaataatta cgggtccttc tgtcgcaaat ccttcttcta aagctttttc
     1501 taattcctct acacttgtag ctcggattcc tttagcgcca aaagcttctg catatttcac
     1561 aaaatcaact ggaccaaagt ctacacctga cgcccgctga tatttcattt tttcttggaa
     1621 ctctaccata ttgtagtgtc catcattcca aatcaaatga acgatattca attttttacg
     1681 taccgccgtt tctaaatcct gggcagagaa taaaaatccc ccatcgccgg aaactgatac
     1741 gatttgtgta tttggacgca ccaatgcagc agaaatcgcc catggcaatg caacaccaag
     1801 cgtttgcata ccattactga ataacaaatg acgaggttca taacttctaa agtggcgtgc
     1861 catccaaata taatgactac ctacatctac ggtaacagtc atatcatctg ttacttttga
     1921 ctgaagcgtg ttaataactt ctaaaggatg caagatacct gcttctcctt ttgattgaac
     1981 aatgtcccgc tccgttaatt ctgcttgaag agatgctaaa tattcttttg catcttctga
     2041 cacttgttga ggctctaatg aacctgtcaa taaatcaaga gtggctgaga tatcaccgat
     2101 caattctctt tcaggctgca taaaaggatc gatttcagct ggcgcttcat cgatcacaat
     2161 gatccgcgca tctttttccg cattccagtt tcttgcttca tattcgattg gatcatagcc
     2221 aatagcaata actagatcac ttcgtttcaa aagcatatct cctggttggt ttctaaataa
     2281 acccacacga ccaaagaaat gcgcttctaa ttcacgtgag atcactccag ctgcttggaa
     2341 tgtctctaca acaggcaatg ctgtctttcc aactaattgc ctgatggcta acgtttcttt
     2401 ttcacttgat cctcgcatac ctactaatag taccggtagt tttgcttctc taatgcgttc
     2461 agctaggtaa cggatatcat ctgcagaagc agaacctaat ttaggatcgc tcaatggctt
     2521 gatgacattc ccttggacag gtgcatccac tacatcttgc gggatactga taaagctcgc
     2581 gccttttttt gaactcttag ccatacgata agcattggca atcacttctg aaattgtttc
     2641 tggatcttgg atttctgcac tgtattttgt gattggctgg aacagtgctg cattatccat
     2701 gctttgatgg gtcaacttca atagatcgct tcgttttact tgtccggcaa ttgctaaaac
     2761 tgggtcacct tctgctgtcg ctgttaccaa acctgtagct aagttactag caccaggacc
     2821 acttgttgcg ataactactc ctggctctcc tgtaatacgt ccgattgctt gtgccataaa
     2881 tgcagcattt tgttcgtgac gagtcacgat taattcagga ccttgatcct ctaattcatt
     2941 aaatacacca tcgatttttg cacctggtat accgaataca taatcaacat gatgattaat
     3001 caaactttct actacagatt tcgaaccttg tttcattttt tcgtccattg aacatcgtcc
     3061 tttttatgct ttatttttaa ttattagtta gaaataatca caaataattg atacagtttc
     3121 taaacctgtt tcattcaaaa gtatagcacg ttttatttca tttgtttagc catatgtttc
     3181 aaaaaatgaa aaagaatgac agtttttaca taaaaactgt cattcttaat tgaaagtttc
     3241 aatttataaa gtctacagat ccatagttct catcaattta cgattgttga agtaaatcaa
     3301 accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga aaaagaagag
     3361 aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt tcaacgtata
     3421 gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca cctgaaaata
     3481 ccagtagttg atgctaaagg aaactgataa acaagcaagt aaaacaaaca acggaatcaa
     3541 ccattttcca taagaaaaac gtataaaaaa gaaacaatca aaaatcatta tcacactaac
     3601 tatccaagca aatagccaat aaaaaaatcc ctttagccaa aattggcgaa acgtctttat
     3661 gaacaaacgt actggtgaaa aacttttttc ttttgctaat tcttctgtca tacctatcat
     3721 cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga cattccttcc
     3781 atctatcgac aaaaggaacg cagctgctat atacaagata ttgacacacc aaaaacaaag
     3841 gctacttgcc aatgattgat acaaccaata aaagaattcc atataaacat ttttgtcaaa
     3901 acgctgctct ttcataatca cctcctattc cttcattcct gatgaagcga tcccttctac
     3961 aaaatatttt tgcatcgcta aaaatataaa agcaacagga agaacagaaa caactgaggc
     4021 agccatgatt aatgcgtact ctgcatcaaa tagcccaata aacattttca accctaactg
     4081 gattgtttta ttttccgtag aagacaaata aatgaatggc cccatataat cgttccatgt
     4141 attaacgaat gtgatgatcg tcaaactggc aatcgccggt tttgtaagag gtaaaatgat
     4201 cttgcgatag attccccact ctgataagcc atcgatccgc gcactctcac ataaagaatc
     4261 tgggatacta ctgtagtatt gcttcaacaa gaacacacca aaagcattga acgcttgtaa
     4321 tagcaccaac gaccacaacg tatatgttaa acccactttt ctcatcataa tgaactgcgg
     4381 gatcatataa gactgccaag ggacagcaat cgtgccgata tatgctaaaa ataaaatatc
     4441 gcgcccttta aaatttagtt tagaaaaccc gtaagcagca aaactagaag taaacaactg
     4501 aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca ctaaaggaat
     4561 acgagaccaa ataaccagat aattctctaa atgccaagtt ttagggatcc attgaatcgg
     4621 aatcgtgaac acttcattat ttgctttgaa tgaagcagaa accatccata aaaaaggaat
     4681 gatcgtcaat aaagctaata caatcagcag aatcataccc aaccattgaa taaagggcat
     4741 gttttttgaa cgaatttttg ctttttttct ttcatttcga acacctccaa agttataatc
     4801 ttcatttctt tgtaatgatt acaatccaga acaacggtta ttatagctga ttcatttccg
     4861 ttcttcttat gtttgtaagc gcttatttaa caagttatgt aaatctcttt attttttttg
     4921 atttaaaaat gaaaaaatac aaaagcaaga ctgtcatacg tctcgctttt gtattgaaat
     4981 ttcttcgatt agagatactt attactgttc aaaagcttct ttgaaacggt gaatcaattc
     5041 attgactaca ggtgataaaa tccgattctt tttccagact agcatgcagt tcgtttcaat
     5101 cattgggtca agcggaacga atgtcactcg ctttgaaaga tgacttacag caccttctat
     5161 gactaaagca gcacctacct gattctcaat tagcgcaaat acattgaaaa tcaaattgta
     5221 cgttcctgtg atcagcaact gctccttttt tatgcctaac cagtcagcaa acattcgctg
     5281 tatttctgga cgctttgaga tcaaaagagg tacattcgca aagtcttctt ttgttatcac
     5341 gtcttttttt gctaaaacag aattttctaa aatgagcaat ccccattttt cctttcttgg
     5401 taacaaaatc gtttcgtatt tctctgtatc tactggatct agcaaaatcg ctaaatctaa
     5461 caggcttttt tctagtcact ccgtgatatc atcacttgtt ccgctgtgga tatgaaattg
     5521 aacgctggga taatcacgga tcaacttttc tagtaatagt gccaatgtat tcgaattatc
     5581 agcttccaca cagccgatcg taaatcggcc gctgaataat tgtttttttc tgttctcgaa
     5641 ttcttgagtg gtttgattcg ttaaatctaa aatttcttct gctcggcttt tttagaaaat
     5701 aacctgcttc tgtcaagatc attttgcggt tttcgcgtac aaacagttct gttcctagtt
     5761 cctcttctaa ctccttcagt tgtcggctta aggttggttg tgtaagatgt agaacttctg
     5821 cggcttgaga gattgttcct tcttctgcaa ttgtccaaaa atattttagt aagcgtaact
     5881 ccatagcgat gctccttgat ttttttactt gatacattcc attttatttc tagataacac
     5941 acgatcatat ttcttgattt ttccttgtaa acgttccaaa cactcttgca tttcagcaat
     6001 ttctttcgtc aaaagttcac tttcttcaat caaaagctta cgccgctcaa atacagttgc
     6061 ttctccttcg ctagataggc gaatatattc aatcaaagac tcgatcgata atccggcatt
     6121 tcgcatacag atcgtaaagt cgacccattt taggtcttct tctgtataat cacggattcc
     6181 gccttttgtc cgattaactg gtggaatcaa cccgattcgt tcataatagc gcaatgtatc
     6241 ggccgaaatg cccttttctt cactgacttg tttgatattc atctgatcac tctttctctt
     6301 aatttcttgc cactttactt atgtatttta tttattctac aactttaagt gtactccaag
     6361 tcaatactca tttctattaa aactaagctg gattatctat atcggttgtt tgtgtattat
     6421 ttcaaataaa aaagtcttgt ttttttattc ttttttggat atacttttaa caatttaggg
     6481 aaattttagg aaaaggggta taaaaatgac aacgtatcag cgaaaggtat tattatcgac
     6541 atcggcggga attgctttag agaatatgga tattatgttt ttagcttttt cattatcttc
     6601 aatgattact tcttttcata ttagtggtac gcaagcgggt ttcatttcaa cgatcactaa
     6661 tttggggatg ctcattggag gtattttttt tggcatcata gctgataaat atggacgtgt
     6721 gaaagtattt tctcaaacgg tccttctgtt ttcgattgct tctttattga tgtattttgc
     6781 ttcaaacatc tacttggttt atctctttcg ctttatcgca ggtgtaggag cgggaggaga
     6841 atacggggcg tgtatgtcct tgatttccga aagtttttca aaaaaacaaa ttggacgagc
     6901 ttcttcggtt gctggtattg gtgcgcaggt cggtgcggct ttggctgcga ttttagctgc
     6961 tgtgatcatt ccaacgtttg gttggaagat gttgtatgtc gtaggtgtac tgccggtgtt
     7021 gatggtcttg ttcattcgtc gcggcttgac tgaaccaaag gaattcaaat ctgtacaatc
     7081 tgccgaaaag aaaaccaaac ttagcagact gtttgaatca aaacatcttg cttggcaaac
     7141 atgtggcctt agtatcatgg taactgtcca aattgccggc tattttgggt taatgaattg
     7201 gctgccttca attatgcaaa atcgattagg gctttcggtt tctgggtctt cattgtggat
     7261 ggtcagtact attttaggca tgtctttggg gatgatgact tttggcataa tcatggataa
     7321 attggggcct aaactagcct tttctttgtt tttaatttgt tcatccttat ctgtctatct
     7381 acttgtgtta gcccatagcc agtggagttt ggtagcagcc gctgtgatag tcggctactt
     7441 tatcaatggc atgtatggtg gatatggcgc agtgattagt agtttgtatc caacagaaat
     7501 ccgcgcgacg gcgaataatt tcattatgaa ccttggacga gctgttggtg gtttttcttc
     7561 tatcgtcatc ggtttcttga tggatcatta tagtttaact gccgtaatct tgtttttgag
     7621 cggtatttac ctagtcagtc tgctggtctt gacaactctt actggtgtta cagcattaag
     7681 ggaaagcttg cagcattgat agttgagtct gtaaatgaca agaaagaggt gtggcaaaag
     7741 cttgcatctg cggtaataag accagccaaa tcaaaaacat gagacactgt ttccgtttgg
     7801 ctggtcgtat tacttgatgc agggcagctt tttcaactgc taatttcctt ctatctggcg
     7861 gcgactattc tttgacgtcc gtcatttcaa agtactttac taataactta gcataacgaa
     7921 aaactgtgga tacactcgta tattgagcgt ctgcataatc ttccaaattt ttctcaggat
     7981 gttgatacat gaattcaatc aaatgataat tatatgattt tctgtagaaa tgaagataaa
     8041 taatgtagat tgggaagttc tcctgctttt ccatagaaaa gcgccgatta ttctcattct
     8101 tcaaaatgac cttatcagga tatttttgga acaattcttc taaatctgtt agataattca
     8161 agatagactt acttgatacc ttcaattcgt tttgtagttc ttctattgaa caccaacgtt
     8221 ctgtttcaag cagtaaatga atcattttaa ttttcttttg agaaactttt gttaaaaaag
     8281 tctgcatatt ttctcctcct gtatctactt tactatacta catacaagaa aaaaaacgga
     8341 tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat ttctcttttt
     8401 ttcctaagta ttctattgat atgaatcatt taatcgttta ttatataaat gtcaataatc
     8461 taataataaa ataacgtaca tattttatta ttgcatactt aataacagaa ctatagcaaa
     8521 aaatcccgtg tatcaacacg ggattagatt aagtttcctc aatcactatt cataacacaa
     8581 ttattataac aagtaagtaa acaattgaaa aggacattta ttgaaaacgg ttgtttttta
     8641 cttaaggagg aaatttagcg aaaaaattag ttgcattgtt tttggtcgca ggaagtttgt
     8701 tgagtgggtg tggtgctact acgtctacta ctcaaagcac aagttcagat caggcttcca
     8761 gcagcagtac cacgatctcc gaaagtacgg cagattcatc gaaagaacaa gaacaaattg
     8821 aaaagaaaaa agaagagaaa caaaaagcgg aaaaagctgc actagaaaaa aagtgacaga
     8881 agcggaatct gctttgtcac aagctgaagc aaatcctaca aaagaaaact atgcaactgc
     8941 ttcagcagct atccaagcgc tacctgaaaa caaacaagag ttgacaagcc gtcttgctgc
     9001 aattgattcc acaatcaaag caaaagaagc agaagaagaa cggaaaaagc aagaagaaat
     9061 tgcacgacaa caagcagaac aagcgcaaca agaagcaatc gcacagcaac aagcagaaca
     9121 ggcgaggcaa gaagcaatcg cacagcaaca agcagaacag gcgcgacagc aatcagcagc
     9181 tgaacaggcc tctcagcagg cacaagccgc agctcctgag caaaataatg aacaaactgt
     9241 ctatgtcaca ccgaccggtt caaaatacca tacacataaa tgtggaaatg gtacgtatac
     9301 ccctgccact ttaagcgaag ctcaagctcg tggtttaacc ccttgctcca aatgttatgg
     9361 aaactaattc aaaagttata ctataataat aaaattcttc tgaaacataa aaaaatagaa
     9421 gatggcgttc cagatagtaa accaaaaata acagtttaca cgtccatttc gtcagcttct
     9481 ctcttttttc atttagtctg ttccctctgc ttttttggac atcttcattc attctttctc
     9541 aatcaaatag tacacctctt tttcttttcg cagtttatat cttccttttt tcaatttcat
     9601 tgaaaactgt ttcgaatgtt tatgttttac atggatttta agcaggagct cctcatgttg
     9661 caagcgatag ttcctgcctt ttaaatcaga aaatctcact agtccgtatt catcagaagc
     9721 tttcacaaat ggaacttttt ctctagtaat atagtgtttg ccgttgagcg tttttacaga
     9781 aaagaccatt ccttcgattg gtcgtccgtt tgtgttcttt acataaaaag ataaaggata
     9841 gtttcgctta gcgatggcca aagaatctaa ataagcgatg agtagccaaa ctaagccgat
     9901 tgatccaagt agtataccga taaaccaaat aaaaaatttc cattttttcc attgatcaat
     9961 tccttggatc gctttttttg cctcttttgc ttgataagga atacgatgcc ccgtcacaag
    10021 cagtcgatgt gtattgacca tataaggagt acaggtaagc aaagtcacga gatctctgcc
    10081 tttttggata cccaaagaat ctacttcatc aggtaagacc acttggattt tttctacttg
    10141 atacgccaat gtctttccat tgatctggat gaaaaactgg tctccttttt tcaacttagg
    10201 caaatctgta aataattttg cttctgggag tccacgatgc ccagaaagaa cggcatgggt
    10261 gtttttcccg ccagtcggat aagaactttc ttccaataat gacgttcctt tttgcaaaaa
    10321 tatttccgtt gtctgatcga atataggtaa gctgacattg atttttggga tttcgatgac
    10381 tccaagcata tgttccataa aaaaggcatt actaggtaaa tcttttaatg cttttgcatc
    10441 aactgcttgg ttgaaactgc ctatccctgg tgatacgttt tgctctgcca actgttggtt
    10501 tttctttgtc attttttcct gctgtttttt tatttcctct gaattcttat tcgatgcttg
    10561 cttttgataa tggatgatca tttgctgggc aagaaaatca ttcagtgaat cttctacaaa
    10621 aggataagaa aagatcccga tcccaacgac taaaatgatt gccatgaaga tgtccagtat
    10681 tcttattctg cttttattca tcatgtccac ctattttcta atatgtatat gaagaggttg
    10741 tgaaatcagc gccttgacct gagtattagc gaagcaaacg gcaaaatagt tcctcctatt
    10801 ttgccgtttg ttgagcgaat accaagagac cgaccacttg tcacaacctt tctccccatt
    10861 tgaatcacta tcgtttattt atcgtatgat ttttgtttcc aatagtttaa gcttctgttt
    10921 cttgatggcg ttttttgaaa tagatcactg ctcctccaac taaagcagta cctaccaaga
    10981 tgaatacgat gatccccata ccaccagttg aaggcaagat tcctttaggt tggttatcga
    11041 tcgttgtttc atcagaagtt gttggatcat catctactgt aaattcagtg tacgcagctt
    11101 cattcttagg taatgcgtag ccttctggtg cttttgtttc ttgaaggcag tatgcccctt
    11161 gtttcagccc ttcgattgaa aatttccctt ctgaatcaga aatcaatttc acttcttccc
    11221 agtcagaacc tgtgacttca tctgaccaag catatgggta ttctcctgag gcaaggtttg
    11281 ctattttttt cattaattta acagttggat cttttgctct tagaatagaa aattctgcat
    11341 cggctaattt ttcttgcgtg ttcacatctt gtttgatgaa gtggaaatca ccgtagtttt
    11401 ggacattttc tacagtaatc gtgttatccg tagttgtatt ttcccgattg actacaccgt
    11461 ttggattccc aagattggct tcatctgcgc catggatgat accggttgtt tgttgcaatt
    11521 ttccgttttt tactataaaa gtagtttctt tttgtggatt ggttaattga taaccatctg
    11581 gtgcttttgt ttcaactaaa gtataattgc cgtctattaa tccattgaca ctgatggatc
    11641 cttcagtatc agttgtgaat gtcaacgctg actcagcagg atcatagaga gcgacaccag
    11701 tgttttcatc aaaaccggtc acgaattgat tctctgaatt tttgatctgg aattgcgccc
    11761 ctgctaactt atctacccct gaaaagttcc cagctttctg gattttgata ttgctttctt
    11821 tgatgacatt tttagcatat aggtgaatcg tgtctaaagg tttgtcaggg tctagggtaa
    11881 gcttaacacg atatttggcg acgtttcgac accgggcttt ggtgtttcta caaagacgta
    11941 tacagcatct ttcccatcag atttacctgg aagagtcagg ttagctaatc cttcaccaga
    12001 agtcgttaca cttgcgaagg gtgtagcgtc tgctggaact gctgtgtctt tacttggata
    12061 gtacgtagca gtgatgtcat atacgttgaa ttcaacgccg ttcaacggtt cgctattcgg
    12121 aaatgtcaat tccgttccgt tgtttatttg attttctggt gctgaagtga atttcaattt
    12181 atgcaaatta accgtaacat tttctggtgt aggtgatacg gctgcagaaa cctctgcagt
    12241 tcctaggccg agaacgaaag gaagcagcat gatagcagtt cctaaaaacg tccatatttt
    12301 tgttttgatg tttttcattt tatttccctc ctattgtttc ctttttgttg ttttttataa
    12361 aaccaagctg tccctgttga aaggactagc aataacccta aaattgagaa acctccaagc
    12421 atctcttcgc ttgtttttgg aaagcttcct ccattttttt catttgatgg accatttgtc
    12481 tgcatctgaa ctggcgtttt tccattattc gtcggatttg cttttggttc gttgatgatt
    12541 tccaatggta taggatgaag cggatcataa ctgccttttt caacttcaaa gagaatggtt
    12601 gattgactca atatatatcc tttaggcggt ttaatctcca ctagttcgta acttccaaaa
    12661 gacaatccat ggacagaaaa tgttccatct ttactggaag taaaaatcgt tgctcttgaa
    12721 tcatctttgg ctacccaatg atttatcccg ttttcttgcg ctaagtattg gtcatttttt
    12781 ttgactagaa attgagcatc tgctagtttt tgtttcgttt gccgatccac tttgacaaat
    12841 tgtttgcctc ctgtttggat ggcaagatgt gtttctactg tttgattgtc agtcgtcaaa
    12901 cgtacatcgt tatcaaatgt cgttttttca ctgttttctt tcaatgtcat ttggtaacgg
    12961 atcgttaatt tttttccagc aaacgaacta agatcttttc cagaatctga taagacaaaa
    13021 ccatgtttta ttttttgtgt gtgaaatgta tgaatcttgt ctccaccaat caaaaaatcg
    13081 atcgtttctg gttctagcca taactgagga tcagctgtat ctttgatcgt ataggttcct
    13141 atctcgttga tgttctcagg aattgttacg gtgatgagat aagggacttt atccccatct
    13201 gcaaaatctg tctgcttatt ctctactgtt tttgttaatg tgagtgcagc aggtattttt
    13261 tgttcgctct ttggatacaa gtggatggtc gtcaatttat gattagatga atctgtcaca
    13321 ggaagcacaa cgacaaatgg tgctgatttt tcttttactt aatctgaagt ctttatttcc
    13381 gtaaatcggt aaacagcgtc acgaccttgc tcatctttgt ccgatgcact aaaagaagca
    13441 atgccctctt cctcgtctac tgtttttgtc acattttctg ccagtatttt ttcactatca
    13501 cttttttgtg ctagttttct ttgtgcgtct tccacactac ttccttctga ccgcagcttg
    13561 taaaattcat ccgagacatc atatatagcg aaagtaacgc cgttcaaccc ttttactcct
    13621 tgaaagttat ccgggatatt tattccgtcg ttttgactga tacttggcat cttatcgctt
    13681 tcgaacattc gattatgcaa agtaaatgta ataggtgatt ctctagctga cacagtcgga
    13741 gaacctataa aaaagaccag taggataaaa aagagaacag caaatatatc cacacgtaca
    13801 ttttttgttt ttttcatcgc tgttcacctg cttttttctt acgatggata aaataaaaga
    13861 agccagaaaa aataaagatc agtcctaaaa agacaaaggg cagtagacct atcccacctg
    13921 tcgaaggaag caagagagtc ggattgatcg tcaacttagc ggtatctgtt gcgataatcg
    13981 tataattatt gtttacactt ccttcgccta aactaatcgt tactatttgt gatccaatat
    14041 ctgtccggct gttaactgtt agggtataat ccaatgattc gcctaagaca acaccggtaa
    14101 cgtggagatt tccaaccgaa atcgagtgac tttgggcatc ataattaaag gcagcgtcat
    14161 cgacagtaat cactgcgtta gctttataga tggtataagt tcctgatgtg ttggctatat
    14221 cagtaaatga caacttggga tttgctgctt tgactttgtc tattccgcta ttcgttagtt
    14281 taattgtata gctgccagca ttaaccatat tttttgctga ttgtgcatct gctgtgtaat
    14341 ccatactgct aagtgccact tgtactggag taagcgaact agtgacaccc tctgctagct
    14401 tgacatggat tgacgggaca cctgcttttc catcatatgt cttactgcca ctgagctcta
    14461 ccgcaccgct gatttgctta atcgtataga catcagatgt ctgcacgata gtaaataaat
    14521 agtttgttct ggcagctagt ttagctagtc cgctttttgt caattgccaa gacgtatagg
    14581 ttccaatttc agtaggcgca gttacttcaa ctcccttttc attcatcgct ttgaaatcag
    14641 ttggattcca tgtgatttga tcaatactga gatcattgtt taaaagggat gttgattctg
    14701 ttttatcatt aaaggcatat ttaacgatcg gtacaacaga aagaccatca tagtacttac
    14761 tgccatttgc attgattgat actgttgcat ttgtcttata atataggatg acttcagaaa
    14821 tctgttttcc atttgcgtcc tttacgatta catttccatt tgtatctaaa ctagctaaaa
    14881 cactcattgc tgattggttc tttgtttcta tcgctgaaac agctgtaaag gtatatccgt
    14941 tgatagtagg cgtattagtc gtcaggtctt gtgcagcact tccgtaagta agattcatta
    15001 ttttagaagg agcaatcatc tgtccaaaat agtcttgata cttaatagtg attggtgttt
    15061 gtttgcctgt ataggtcacc gtatcgacgt tactttctag ggctgtgaaa gaaccgtttg
    15121 tttgtgcaaa agaatagccc gttactgctg ttggagtgat aaccactttg tcacccacat
    15181 ggattccaga taatgtagtc ccgtacgttc cgatttttgc tgctccacca ttgactgtat
    15241 aagaaaaagt tcctgtccga ttcgcattga cctgaaccgt tgctgcttga taggttggaa
    15301 aatttgaaac atttccctta ccatcaacga tctgcccatt tttgatcgag taccccgtga
    15361 atggaatatc atttgctaac tgtataccga ttgaaaaagt actcatacct cctccggcag
    15421 gaagccctcc ccatgttgag ggtgtaaaag aataactttt atccgcattg ggtgtaactg
    15481 aatcgatcgt atttccagcg gtagccaccc ccatatagat actttttggg aatgtgatag
    15541 tagtaccatt tactgtggag ccaataaaca gattatggat atctggacgg ttatcaataa
    15601 aagcgattgc tttggttacg ccttgtaacg cagttttatc cgcactcatc gatccgttaa
    15661 ccgcaacaat gactccatct ttggcatata tcactgtata tccatctggt atggtatgtc
    15721 cgttgaaatt cgcagtggcc gcaacagtac taaatgtctg gcttctacct gtttctgaat
    15781 aagctttctc ccctgctgct tttgctgctg tgatttcttt ttctgtcgga tcaatcagtt
    15841 gagtcgagga tgtggttgag gttgtctcgt tgttgctaga ctgattggtg ctttctatgg
    15901 actgtatttc agaatagggt ggactatttg tctcctctgt cgtttggtta gcaaaatttg
    15961 acgtttctgt ataaacgttt acataaatac ttgataagct ccctaagaaa gtaaaaatca
    16021 agatgatcga aatcataatg ggatgtaagt attttctttt agatacagct ttgagtctag
    16081 aaacaaaatg tttttgacat atacgttgga ttttattttg agttttctta atcattttca
    16141 tccctcctct caataaaaaa tcatcaaata atcctttaca cgttattcta aatttaattt
    16201 ttattatttt attgatgtat agaaagtttg gggctttttt tctcttttta ttctaaggtt
    16261 attttagtct tgtcctttaa tacaaagatt ttatttttaa ataaaaaata ctcttttttt
    16321 acgattattg aaaaaaaa
//
LOCUS       DAYYNG010000055        16274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000055 DAYYNG010000000
VERSION     DAYYNG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.055"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..197
                     /locus_tag="ACHBFA_001417"
     CDS             <1..197
                     /locus_tag="ACHBFA_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9394916.1"
                     /translation="FPKEEAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEES
                     NSVRKSQVVEKMGRHSVIKG"
     gene            complement(482..1303)
                     /locus_tag="ACHBFA_001418"
     CDS             complement(482..1303)
                     /locus_tag="ACHBFA_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9394917.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1400..1678)
                     /locus_tag="ACHBFA_001419"
     CDS             complement(1400..1678)
                     /locus_tag="ACHBFA_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394918.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            1946..3955
                     /gene="metG"
                     /locus_tag="ACHBFA_001420"
     CDS             1946..3955
                     /gene="metG"
                     /locus_tag="ACHBFA_001420"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9394919.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(4434..5021)
                     /locus_tag="ACHBFA_001421"
     CDS             complement(4434..5021)
                     /locus_tag="ACHBFA_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394920.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            5196..6518
                     /locus_tag="ACHBFA_001422"
     CDS             5196..6518
                     /locus_tag="ACHBFA_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9394921.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6673..7449
                     /locus_tag="ACHBFA_001423"
     CDS             6673..7449
                     /locus_tag="ACHBFA_001423"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9394922.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            7442..8011
                     /gene="rnmV"
                     /locus_tag="ACHBFA_001424"
     CDS             7442..8011
                     /gene="rnmV"
                     /locus_tag="ACHBFA_001424"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9394923.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            8004..8888
                     /gene="rsmA"
                     /locus_tag="ACHBFA_001425"
     CDS             8004..8888
                     /gene="rsmA"
                     /locus_tag="ACHBFA_001425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9394924.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            9339..9968
                     /locus_tag="ACHBFA_001426"
     CDS             9339..9968
                     /locus_tag="ACHBFA_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394925.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(10092..11201)
                     /locus_tag="ACHBFA_001427"
     CDS             complement(10092..11201)
                     /locus_tag="ACHBFA_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394926.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            11410..11832
                     /gene="mgsA"
                     /locus_tag="ACHBFA_001428"
     CDS             11410..11832
                     /gene="mgsA"
                     /locus_tag="ACHBFA_001428"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM9394927.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      12019..12117
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            12155..12691
                     /locus_tag="ACHBFA_001429"
     CDS             12155..12691
                     /locus_tag="ACHBFA_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9394928.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(12731..14494)
                     /locus_tag="ACHBFA_001430"
     CDS             complement(12731..14494)
                     /locus_tag="ACHBFA_001430"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394929.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(14497..16212)
                     /locus_tag="ACHBFA_001431"
     CDS             complement(14497..16212)
                     /locus_tag="ACHBFA_001431"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9394930.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQDTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         5181 a         2983 c         3272 g         4838 t
ORIGIN      
        1 cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat aaattaataa
       61 caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc aataccgacg
      121 gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt
      181 cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt agccttgtta
      241 ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa agctcactaa
      301 ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt ctgctattgt
      361 taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc
      421 acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt tgctcacaac
      481 cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg attcctacag
      541 gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg gaatgatcga
      601 tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc gtaatcgtga
      661 aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg actacacggt
      721 tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg ccagaagact
      781 ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg atcttgttac
      841 tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg attagcggtt
      901 cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga tctcgattag
      961 gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca ccagtttctt
     1021 cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca aagccaccag
     1081 gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa actttcagct
     1141 gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag ggcttgtcat
     1201 attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt gctttatgtt
     1261 cataatataa tttttcttct tctttagaag caaaacggtt catctgacca cctccattga
     1321 cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa ggatttactt
     1381 gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga ttgaataaaa
     1441 agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca aagaagccac
     1501 aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc ataaaactgt
     1561 tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag ctgaatatta
     1621 aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga
     1681 ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga ggggagaaat
     1741 aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat tcaacctatt
     1801 catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc tttttcattt
     1861 tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac gaattcgcat
     1921 catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata tcactacgcc
     1981 aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga ttgcatgtga
     2041 cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca ctggagtgga
     2101 tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata
     2161 tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata tcagttatga
     2221 taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa tatttgatcg
     2281 tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact ctgtttctga
     2341 cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg aaggtaacgt
     2401 gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag aatcatactt
     2461 cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac atcccgaatt
     2521 catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac caggattaga
     2581 ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga gtgatccgaa
     2641 acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac taggatatgg
     2701 ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa
     2761 agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc ttgatttgcc
     2821 tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa aaatgtctaa
     2881 atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt tagatgcact
     2941 ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga
     3001 ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat tgaatcgaac
     3061 gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct cacttgtcac
     3121 tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt accatgatgc
     3181 aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga tttctcgtgc
     3241 aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag aaaagaaagc
     3301 tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag cgatattact
     3361 tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat tggactctga
     3421 aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa aagtagttgc
     3481 aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt ttattcaaga
     3541 aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc cagaagaaac
     3601 agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa aagtagaact
     3661 aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt tgctgcaatt
     3721 ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta
     3781 tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg
     3841 aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac cagatggctc
     3901 tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg catagaaatt
     3961 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
     4021 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
     4081 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
     4141 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
     4201 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
     4261 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
     4321 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
     4381 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
     4441 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
     4501 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
     4561 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
     4621 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
     4681 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
     4741 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
     4801 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
     4861 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
     4921 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
     4981 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
     5041 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
     5101 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
     5161 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
     5221 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
     5281 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
     5341 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
     5401 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
     5461 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
     5521 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
     5581 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
     5641 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
     5701 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
     5761 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
     5821 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
     5881 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
     5941 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
     6001 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
     6061 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
     6121 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
     6181 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
     6241 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
     6301 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
     6361 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
     6421 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
     6481 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
     6541 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
     6601 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
     6661 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
     6721 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
     6781 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
     6841 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
     6901 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
     6961 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
     7021 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
     7081 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
     7141 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
     7201 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
     7261 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
     7321 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
     7381 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
     7441 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
     7501 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
     7561 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
     7621 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
     7681 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
     7741 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
     7801 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
     7861 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
     7921 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
     7981 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
     8041 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
     8101 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
     8161 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
     8221 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
     8281 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
     8341 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
     8401 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
     8461 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
     8521 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
     8581 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
     8641 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
     8701 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
     8761 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
     8821 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
     8881 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
     8941 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
     9001 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
     9061 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
     9121 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
     9181 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
     9241 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
     9301 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
     9361 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
     9421 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
     9481 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
     9541 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
     9601 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
     9661 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
     9721 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
     9781 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
     9841 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
     9901 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
     9961 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
    10021 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
    10081 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
    10141 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
    10201 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
    10261 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
    10321 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
    10381 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
    10441 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
    10501 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
    10561 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
    10621 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
    10681 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
    10741 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
    10801 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
    10861 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
    10921 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
    10981 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
    11041 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
    11101 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
    11161 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
    11221 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
    11281 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
    11341 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
    11401 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
    11461 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
    11521 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
    11581 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
    11641 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
    11701 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
    11761 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
    11821 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
    11881 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
    11941 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
    12001 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
    12061 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
    12121 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
    12181 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
    12241 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
    12301 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
    12361 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
    12421 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
    12481 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
    12541 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
    12601 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
    12661 tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca tcccagaaga
    12721 ctgcttgttt ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa taaccatctt
    12781 tttgaagcag ctcatcatgg gtgcccattt cgacaatagc tccctgatcc atcactaata
    12841 tctggtccgc atttttgatt gtggacaggc gatgggcaat cacaaaactg gtttttcctt
    12901 ccatcattgc taagaaggct tgttggattt ttttctctgt caatgtatcg actgagctgg
    12961 tagcttcatc taagatcaac atagggggat tactgatcat cgttcgagca atcgtcaaca
    13021 gttggcgttg tccatctgat atctttaacc cttgaccgcc aatcttagta tctaattttt
    13081 ccggcaaacg ttctacaaat tcatacatat aagaggcttt tagggcatca ttgatttctg
    13141 cgtcacttgc ttccggattc ccaaaacgaa gattttctcg caatgtactg tcaaatagcc
    13201 aagtatcttg taacaccatt ccaaaacttt tacgtaatgt atcacgttgg atagagcgga
    13261 tgtcatggtc atcgactgta attgttcctt ttgttgtgtc ataaaaacgc atcagcaaat
    13321 taacaagtgt ggattttccg gcccctgttt ttcctactat agcaatggtt tgacctggat
    13381 aagcagtaaa attgaaatct tcgatcaaac gctgcttggg gtcataagaa aaagaaacat
    13441 gatcaaaaga gacttttcct ttgatatttt tgggttccaa ggcataggca tctggcaaat
    13501 caggcttttc ttctggctga tcgatcaaat caaatgctcg ttctagacca gaaaaagcgg
    13561 tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac tgacttgagt
    13621 agatcgtaaa gctggaaatc acaccaacag tgattgacga accactcttc aatgccaata
    13681 ggccgccaac taaaccaatc gataaataag caaggtgatc tacaaaacga gaagacgggt
    13741 ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc atatagatca gcatttagca
    13801 gttcaaattg ctgttgacta gcttcttctt gctgaaaagc tttcacgatt ttctggttac
    13861 cgattcgttc ggtcacaaag cccgagatat ccccaataat cctttgctgt ctatcgaaat
    13921 ttgtttgtga agcacgagca acgatccaac tcacaaggaa aataattggt gtagaacaaa
    13981 gaacgaccaa tgtcagaagt ggacttagac gcatcataaa aataaacgaa agaatgatga
    14041 ttgccactcc agaaaataat tgattgaagg ccgcagaaat tgcaatggaa atattgtcca
    14101 tatcatttgt aaatcgactg acgatatttc cgtgggagtt ttgatcataa taacgaagtg
    14161 gcaatcgatt cagatgagca aatgcatctt ttcttaattt tgcaacggat aagtaagcta
    14221 accgattgct taacacttgg atgagccatt gactgactac gttgattaaa acgataccac
    14281 cgaatagaat aagtaaatgg aacagtcgat tgaagcggac ctgtccttct ccaatcattt
    14341 gatcgatact ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca ttgatgattc
    14401 ctagtatcac agctagccaa atgtcttttg cataggcagt aaggtaagga atgaatcggc
    14461 ggagcgtctg caaggataat ttttttactt tcataactat tcttcctcct gtgatgcgac
    14521 gatttcctga tattctgctg attgagcaag taattcttca tgggtatcct gagcagcaat
    14581 cttaccttga tccatcacta agatatgatg tgcttcttga atagagcgga cacgttggga
    14641 gataataatc actgtactgc caagataagt ctgtagcgcc ttacgcaaat ccaagtctgt
    14701 ttgataatcc aacgcactta aagagtcatc taaaatcagt aatgctggct tggcaatcaa
    14761 agctcttgca atcgtcagtc gctgtttttg tccgccagag aaatttttcc cattttccat
    14821 gacttttgta tctaaaccat ttggaagctg acggacaaat tcttctgcct gagcgatttt
    14881 cagagcttcc caacattctt tttctgtggc gtctttctta ccccattgaa ggttttcacg
    14941 aatagtcccg gtaaatagta cagaagtttg gggaacaaga gcaatttgct gacggagatg
    15001 atccagctgc caagaacgaa catccattcc gtccattata actgtaccgg aactgacatc
    15061 atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg gaccaatgat
    15121 tccaaggact gtatttcgct tcagctgaaa tgtaatattt tggagagaaa gaccagaatc
    15181 aggagtataa cggaagtcaa catggtcaaa agcgataatt gcttcagaag aaaaatcggg
    15241 agtagtactg acctcagctt caagaatcga attttctgtg tctagcacct ctttcacccg
    15301 atttcctgaa gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa cgatcagtgc
    15361 caaaagcatt tgattcatat aattgatcaa agcaagaact tgtccttgtt gcaagctacc
    15421 aatatttact ttgatcccgc caactgtcag caaacagatg atcccgacat tcatgatcaa
    15481 ggttgtagca ggtgataata gggcagagat attggaaaca cgtaaataat tgttcgctag
    15541 atcatttgtg cttttatcga acttctctgt ttcttttttt gtctgcgcaa atgcgcggat
    15601 gacacgaaca ccgctaagat tctgactaac gtgttcattg agctgatcta gtttttcttg
    15661 cactttttgg tagagaggaa cggttttttt gatgataaaa aataaaatga ttgagaaaat
    15721 aggcaataat gctaagaaaa acagacctac ttcccagtcg atcacaaaag ccatgaccac
    15781 tgacccgata cttaaaaacg gtgctctgat caataaacga atgaccattg ctaaagctag
    15841 ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc caaaatgatt
    15901 caactcgttg tgagagaaag tattgatttt tttgattagc tgattacgta actctgtacc
    15961 aaatccttgt gaagctactg aagcatagta ttggcaaatg atagcacaaa tcagaccaat
    16021 cacagacata gcgaccatcc ataagcccat ttcaatgatt tttcctcggt cattcatttt
    16081 tagtccatta tcgatcaagg aagccatcat taaaggcagg acaagttcga agacagcttc
    16141 aagaaatttg aagaacgggc ccaaaatgat ttgcttacga tagtcttttg cataacgtaa
    16201 taactgaatc atatgattcc tcctattatt tatttgtcca caattgaaaa aacaagtact
    16261 accacattgt agct
//
LOCUS       DAYYNG010000056        16204 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000056 DAYYNG010000000
VERSION     DAYYNG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.056"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1628)
                     /locus_tag="ACHBFA_001432"
     CDS             complement(45..1628)
                     /locus_tag="ACHBFA_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394931.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1750..2334
                     /locus_tag="ACHBFA_001433"
     CDS             1750..2334
                     /locus_tag="ACHBFA_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394932.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2345..2590
                     /locus_tag="ACHBFA_001434"
     CDS             2345..2590
                     /locus_tag="ACHBFA_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394933.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2596..3318
                     /locus_tag="ACHBFA_001435"
     CDS             2596..3318
                     /locus_tag="ACHBFA_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394934.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3449..4192
                     /locus_tag="ACHBFA_001436"
     CDS             3449..4192
                     /locus_tag="ACHBFA_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394935.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4198..4563
                     /locus_tag="ACHBFA_001437"
     CDS             4198..4563
                     /locus_tag="ACHBFA_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394936.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4579..5109
                     /locus_tag="ACHBFA_001438"
     CDS             4579..5109
                     /locus_tag="ACHBFA_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394937.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5271..5579
                     /locus_tag="ACHBFA_001439"
     CDS             5271..5579
                     /locus_tag="ACHBFA_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394938.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5710..5994
                     /locus_tag="ACHBFA_001440"
     CDS             5710..5994
                     /locus_tag="ACHBFA_001440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394939.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            6131..6931
                     /locus_tag="ACHBFA_001441"
     CDS             6131..6931
                     /locus_tag="ACHBFA_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394940.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6968..7150
                     /locus_tag="ACHBFA_001442"
     CDS             6968..7150
                     /locus_tag="ACHBFA_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394941.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7261..7578
                     /locus_tag="ACHBFA_001443"
     CDS             7261..7578
                     /locus_tag="ACHBFA_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394942.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7581..7724
                     /locus_tag="ACHBFA_001444"
     CDS             7581..7724
                     /locus_tag="ACHBFA_001444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394943.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7928..8407)
                     /locus_tag="ACHBFA_001445"
     CDS             complement(7928..8407)
                     /locus_tag="ACHBFA_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394944.1"
                     /translation="MTDKTSDLPKLAPKTSRVKRKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8491..9864
                     /locus_tag="ACHBFA_001446"
     CDS             8491..9864
                     /locus_tag="ACHBFA_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9394945.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGILGNWK
                     QESGNDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKNFGGA
                     WWTTEAQLDFMFKGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAVVAVR
                     GGNAKEIWKYMKSKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACATTDD
                     ANSGSGSMGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9887..10414
                     /locus_tag="ACHBFA_001447"
     CDS             9887..10414
                     /locus_tag="ACHBFA_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394946.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10401..10964
                     /locus_tag="ACHBFA_001448"
     CDS             10401..10964
                     /locus_tag="ACHBFA_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394947.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLENLDSYVRAE"
     gene            11387..12124
                     /locus_tag="ACHBFA_001449"
     CDS             11387..12124
                     /locus_tag="ACHBFA_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394948.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            12241..13560
                     /locus_tag="ACHBFA_001450"
     CDS             12241..13560
                     /locus_tag="ACHBFA_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9394949.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKHGRKIRLVVKREVNKFRYCGD
                     IRKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGVVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEDYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRKQYEEEKEKTFKAIRWDWGRQREIYMSKLW"
     gene            13577..13729
                     /locus_tag="ACHBFA_001451"
     CDS             13577..13729
                     /locus_tag="ACHBFA_001451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394950.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDLQ"
     gene            complement(13797..14267)
                     /locus_tag="ACHBFA_001452"
     CDS             complement(13797..14267)
                     /locus_tag="ACHBFA_001452"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9394951.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(14426..14884)
                     /locus_tag="ACHBFA_001453"
     CDS             complement(14426..14884)
                     /locus_tag="ACHBFA_001453"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9394952.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(15055..16200)
                     /locus_tag="ACHBFA_001454"
     CDS             complement(15055..16200)
                     /locus_tag="ACHBFA_001454"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9394953.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLATSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
BASE COUNT         5758 a         2283 c         3145 g         5018 t
ORIGIN      
        1 aaagtaaata tacgctataa aagtgcatct tgtgttataa aaaattattt tattttgtga
       61 acataattta acaattcttt ctcggaattg atttcttttc ttaaatagtc ttcagactta
      121 ttaataatat tatctattat gtctagatac atatcacttt tcgattcagc taattcatac
      181 agttttttta attctttatc ctttaattga aaaccctgat ccatagtcac tgcatttgga
      241 tatagaagcc cactataatg tttacgtaaa ttcacttgga atttctgatt ataaaagtct
      301 aacagattag aaataaaagg cggaaaatta ctgtttttgt aaaattgatt atttccattt
      361 aggttgtata gtaatttgtt aaattgctcc tgatttaatt tattgtaaat ccatggtaat
      421 acttccatat ccttatctgc taacctgaat gaactagcat cggtaagatg ttcgatcata
      481 aaatctatga acatctcttt ttcaccaaaa tataaatatt gattatatat tgtttctaat
      541 gttgaagatg gtagcgaaga aactctcatt ttttgctgga gtataggatc aactagacta
      601 tttaagtgat tttgtaattc ttcattgttg tttcctagaa ctgcaaggga acgtaaacgg
      661 tagtaatatt ctatttctgt tagtacttgt tgtttcggtt taaaatcatt tattgatatt
      721 tgtaaatttt gaattgtctc gtctgaaaaa tcatgttgta attctggatg ggtagctata
      781 aaagtaagta aaaagaaccc ttttgtatac gtgatctgtt gattttcacc aagtgattta
      841 ttaatttcct ctcttgattc agctgtgaac ctatactttg caatttcttt ttttagaata
      901 tttaaataga attctttatt atcaaaaatt agggcattaa gtagtctgaa agtactttcc
      961 ctatctctat ctgcttcctc atttccataa gataattgaa aaacaaactt aaaggctgtc
     1021 ttacataggt tttctttggc tttttcgctt agtttattta gatagctatt ttttagtgca
     1081 gagtgaaatt cttcaggtgt aacagacata gcatgtatgc gacttgcata ttgtataatt
     1141 tcgatcgtta ctttttttcc taatattgga ttttttagga atataatttc ataggacttc
     1201 cgtataacat cctgacaagt ttcttttgtt atatctctta attcccaagt attaccgttg
     1261 taagtgacta caggatgagc tgaataattt ctttgatcct taatatattt taaatcctca
     1321 tattcatagc tatcaattag ctcagttgaa ctataagctt tttctagtag ttccatttcc
     1381 cattttggag agtttggatc actttttctt agtctagcta tatcttctaa aattttctta
     1441 gaagtattat cctcgtaaat atttgctaaa tcagataatt tatcaattaa atctaaaata
     1501 agcgcactgt aattgacagc tattgcagaa cgataatttc cctgataaaa actacttcta
     1561 acttcttcta ataatggaat aattctagga tttttaactt tttttatttc atcatctaat
     1621 ataaacatat aaatctcctt tttttatttg tatgacaagt ttaccataaa aaaaaattaa
     1681 aaagcgagac atatttatgt tgttgtgtta tcataataac ataaatatgt agagtgagat
     1741 gatgaaaaaa tggatttgaa acaatcgttt tacatctggt tatatcctga taatcctatg
     1801 tcaataatgg ctgatatttt tgtagttagc atcttattgt atttatttta tctaattttc
     1861 tttcacaatt taatcgggta catgattctt gattggaaag gaatgttcga tatagaaaga
     1921 aaaccatttg ctaacaatca aacctatacc gtcttcgaaa gtggagattg ggaagaaaat
     1981 gaagttgatg tgatccatga aattggatta gctttatcag gaatacaaga tgctgatgtt
     2041 ttggtatcaa tgacaacgga tactggatac aacgcttatg aggtatttta tcttatgtat
     2101 tttatatggc gtgatggtca agaaattatc aatggtcgtg ctgaaaaaac aatcaattct
     2161 aatactgaaa tttctttttg tatgggtgaa ggggaaatga ttgtatataa agatgggtca
     2221 gcaaacatta aaacagcctt tggtgatatt gaaacaagta ttaaggaact tagggaatat
     2281 gtgcagaacc catataattt tcaatattta gcaggaagaa taaatttagt ttaggagtaa
     2341 aaatatgaaa aaaattataa atcaaccaac aaatcacttg ataattgaag gtgttatttt
     2401 cgtaacaatg tattttactg taggaaatgg catctcaaat acaatcattc aaaaaaatcc
     2461 acaataccta ttatttagtc tttatgcttg gattgtacaa atggtttggg tttccatatt
     2521 gatgctgttg ttttattgga tagtacgggt tattctttta ttgttaaaca aaagaaaaaa
     2581 ggataggtga ataaaatgaa atcaactaaa ttgaaggttg tattgttatc gacagcagca
     2641 ttaataaccc ttacagcatg tggtacaact aatagtgcta ataataacaa agacacaaag
     2701 tctagtagta cgatggttac taaaaacagc actacatcgg catcaacaac aatgtcttca
     2761 atgactgaaa ataatgctga aaagtatcaa aaatacgaag acattaataa aggtcaaaag
     2821 ccggatgaag tttataacaa ctttattaat acagtaaaaa atgatgaaaa ttatgggaat
     2881 gatccattgc aaaataaaaa tctgactatc gttctttaca aacctgattg tccagattgt
     2941 caaaaagcag aatcaaaaat catgcaagaa ataaaagaag gatttagtta tgctccaaca
     3001 ttaatgcatg atattaccat aaataaacca gcttacgagt attacgcagt tgctgtagat
     3061 attactgatg gtgtacctga gtgggtacaa aacattcctg gtctggctga tgagaacaat
     3121 caatatcata cgccaacagt tgcagtagta acaccaaaga aagttaacgg cacactatac
     3181 tgggatgtaa tgggaatgac aacaaataca actgatgaaa tgattgaaag tacaactaaa
     3241 tattatatgg atttgggagc atataaatta tatcaacaat cagagcctgt tatggacgat
     3301 caatccaaag ttaaatagat ctttagggcg taagccctat ttttttaaaa ttaagtgata
     3361 acaaataaat gatttctgaa acaaacgttc ttactcaact actcgaaaca cacaagctaa
     3421 ttaactagat tataaaggag aatgcgtaat gaaagaaatc agtgcaaatg aagctcatat
     3481 tccgcaaaag aatcaggttg gtatatataa ggtgcttcat ggtgaaggaa tttataaaat
     3541 acagttactt accggttaca ataatggtgc aaatgggaag tggttaaagg caacaagtat
     3601 tcgtgaactt agaagtgaag tagaaaaaat taggcttggt ctcacaagtg aagaaatagc
     3661 taaactgaag ggaaataatt cttttgtaat tgaaaaagaa aaggaaaaac atctacttta
     3721 tcgtgagctg aaaattgaag aatactatga aattgattta cctgaaaaat taagaattat
     3781 tgatcaacgt tcagaagaaa aatggaaaac tgaaatatat ccaagcttaa atgactacaa
     3841 gacatcagga aagcccatgt ataactacac aggcttttta acctacacac aatacaaata
     3901 tgcctttcgc aaattaaaag gcattaaaca agctgaaatt gccctgtatg agatgaaaaa
     3961 agtggataga ttgaaattga ttcaagattt agtgcaaaat acatggaatc aagcgattgt
     4021 cgagctagaa aaagaaaatg ggaaaatttt aactaataaa tatctgatcg taaatgaaat
     4081 tgaagaatta attggtttat tttcatgtga aaaaggtgaa gtcttcttga agtcatcacc
     4141 aacctattca attttggaca gtccgtatca gtttatacag gcaaggagat gagttagatg
     4201 tctagcttga aagatgacta tatccaagca aaacaagagt atgaaaaaat acagatgttg
     4261 aaatctgaga aagtcgaatt atttttaaca caaaaattag cagaggatca tattgctaac
     4321 ttagatgcta aaactttaga ggaatatgat cggcagttta atcagataca tcacaatatc
     4381 attttgcttg ttgagcaggc aaagcgaagg tttgaaaaag cggaatatga tctattagat
     4441 caaggaattg tatgcttgga taagggattg cgaagatatt atggtgcaga gttaaaaaag
     4501 caaaatgtag atagaaatgc actaaaacat gaaatcttag aattgataat gcaacgaatt
     4561 tgaagggata gaaagcaaat gtcacaaaac attagtgctg taaaacaatt aatagaagaa
     4621 gatttagaga ccacagttca gagattagcg gaacgaatcc attttgcaaa ttatcaaacc
     4681 atctctaact ggtcaagcag gaacagaaat ccggaagaaa ttccaatgaa atttttttat
     4741 cgaatcggaa aagaatatgg tgttgatccg ataaaaattt tcgagaagac catgatttat
     4801 tactatcatg gaacacctaa cgaagtgtta gaacatcttt tggaagaggc aaaagaaaaa
     4861 tcatcaatat tagagctatc gcaaaatgac aaagttattc aagtgaaatg cgttgatcca
     4921 acaggaaaga ttattgtcgg ttttgatatt gaaagaaaat ccaaaataga ttttattaca
     4981 caattaaaaa agataaataa tttagatatt ctaacgctta gaccagatca tgaacagttg
     5041 tttatgagtt ttaaaaaaca attgaaagaa atacaaaaaa gatataacaa ggtgtttcct
     5101 aatgcgtgaa aagctcaaag aaatcagagt tttttggata attaacaaag aatgtatcat
     5161 cttatatact cttggatttc ttagcatgat cttgttttgg tggttatatt taaaacagta
     5221 attggagagg gtcaagagga tttttatcaa gataaggatg tggaaactgc atgtggaata
     5281 atagattaaa aacaggattg ttattaatag ttatttcctg cgctatgatg attggtatga
     5341 ggatacaaag ggaacaatcc tattttgaag tttctgctaa caatgtaata gaaagatgcc
     5401 attatggtca acatttttgg actggggaag tcagagaaaa tatagatcgt gaatatgttc
     5461 aaagaattgt ttgggatgca tattctatca aagattatcc taaaagttta acgagtagat
     5521 tattttacag tgaaaaagat aatcaaaaac tctcagattt gatgatgaaa aaagtatgac
     5581 tattattacg aaagagaaac agagtgctat attcgagggg aaaaagccta caacaaaagt
     5641 gactattggg gtgacaaggc gaagcgatta actgcaaatt ttagagtgta tgaatcagag
     5701 tttataaact tgacctttct taatagtgtt tttattgatt atgtaattac tacgaaacgc
     5761 attggaagtt attcaagtac ctacgcatcc ttagttccaa tgttaaactc gatgagccgt
     5821 ttcttaaaag aaagagaaaa gaaagagcta cagtatatca aagaaatcat gccggatttt
     5881 gaaatgcacg aatggcaaat tgctttatca gattggaaat tggcgaataa ggttcgtaga
     5941 ataacacctt ttcaagccaa gcgctttttg agagctatga aatttgtgca ataaaaaaag
     6001 ccaccagaca agttgatgga gactaacaca attaccaaca gttgtaattg tgttagctta
     6061 caaattaatt atggtgttta gtcaatccca aattgtgaaa tggcaccaat aattggaaag
     6121 gaaaaatgaa atgtctgaag atttacgaga gctgaagtta cgaaagaagc agtatgatca
     6181 gtatttttcc ggtttgttga ataaacatat tgaagaagga cttgatcatg attttggaaa
     6241 agatgaatat tgtttatatt gcgaacgtta caatgaaaaa atgtctgaat atctacagct
     6301 gaaaaaagag atacagaaat tagagaaaaa agtacatgct tggaagtata aagattccat
     6361 gtctggttat gttgaatctc aaaataagcc aagaaaaaag aagcaagcgt atgttctaaa
     6421 gatggtaaat ggggaagagt taattgtatt agcgtacagt gaagaagagg ttcgagaact
     6481 cgttgaaaca cctgtcagga aaaaggttga aacaataaat gagatagacg acagccaata
     6541 caatagcatc tatattgaaa caagtcatgg tggatataag aaattagcaa ctttccttag
     6601 aagttctaaa gttgggataa tttctaaaaa agacataagt gctgttaccg atagtttttg
     6661 tactttatct gttcctaaag cttatttcca atacgatagt aaaagatttg aacgcttaga
     6721 gaaattctgt agaccgtata ctgtattgaa aactaaaaaa tttaataaaa ctaaaagagt
     6781 ttctatacag ttaccgactg gtcaaatctc attaggtgaa aaagactatc tgataaaagt
     6841 aggaaaagag ttaattataa aagtaccgga acacttattt aaagaaaatt ttgagatacg
     6901 tgaaaccttg gtatggaaag aagaaaaata gtttggaaga catagaatgg tataagggga
     6961 aattgaaatg gcagtaaata aaatattgca ggtactaatc atagacgaat ttggcggagg
     7021 agaatggttt gatgtgcagt ccatagactt tgaagaagaa gtcgtagtta tagaaaatat
     7081 ttacggcgaa gtatattcat attcgcttga aggtgagaat attgaggatt tacggttgat
     7141 tggtgagtag aaaaaaacag aaatatcaaa atggagataa aaattaatag catgtaaaga
     7201 tagtcatgct tgggttaaaa atgttaaaat tcaaaaaaat gagtcagaag ggataaaagt
     7261 atgaaaagag aggaatacaa acaaagatta aacgaactgc ttgaagagga cgaaacatta
     7321 actcatggtt cgcctgatga aattctatac atgattgata atatggtgat ctttggtgga
     7381 tatgaacttg gtaataggtc agttgatcat aatattttag aatttgatga tgtatcatgg
     7441 gaagaaattc ttgattgggg aattttggcg gtacctgaaa ctaaaaccta tattagtgac
     7501 actatggttc ctttttttga ggaattggat tataaaagac ttcccaaaaa tgaaaatcat
     7561 atactgggag gaaactaaca atgttaagtg acttatttat tttactgatc tatctaggtg
     7621 taagtgaaat tattgcgtat tttttgtttg tcattgctaa ttatggtatt tccagtgcaa
     7681 tcgttcaaat gtttagatta ccagtaagtg aactaaaaaa atgatgagat tatcagcata
     7741 tcttttaatg tttttgaatg tggtcacgtt aatctttgta gtaatcggtg tgactttcga
     7801 tttaatggaa tagtgggaaa tctataatgg aaaaaagaga cacgaatgaa catatttttt
     7861 gaatcaatat tgttgatggt ttttgcgatc agcgcttacc agaaatctaa aaaagagtaa
     7921 tgattgttta atcatcactc tttttttgta gggaaattga aatatgtgga gcttgtattg
     7981 gtttagttag gattgcttgg ctataaggag ctagttcttt atcaaaacca tcaaagtagg
     8041 ttttcagtag cgatttaact gatcgctcat cttgtttgat ttggataatt tcagcatcca
     8101 aatcggttgt gagatcagga gcttttgatg cgattacctg atttttttta tcttccaact
     8161 ctcttaggct gtttaacaag ttgtaaaaag aatcattttg attgttcatt ccaacgtgag
     8221 ttgcaatttt aacagaagtt tcgtccgtat tgtcatcaaa agaactcatt ggaatatcaa
     8281 gtagtaacga ggatgtttct tttactgact ttttctttac agcatttttt gtttttccat
     8341 ctttttttct ctttactcga gatgtttttg gagctagttt aggtaaatca cttgttttat
     8401 cagtcattta ttagcttttc ctttctatta atttatgtta ttatattatc ataataacat
     8461 aaaaaagtac actttttggg ggcgtagtta ttgaaaaaat ctaagagaaa gattgccacg
     8521 ttgcttatgt taatggtgca atttacaggg attgcaatga gtgtaatcac aataaatccg
     8581 caagtgatac atgctgatgg gatagctact tatgatgcac aatttaactg tactactgaa
     8641 ggtagtgggt caggaacaga tagcgatgga aacgatactg gaagtggcgc tgtatcaggt
     8701 gatgtagcaa aaaatattga tgatatttac aaatttatga atggggaata tgggctatca
     8761 ggtgctacta tcgcaggtat tctaggtaac tggaaacaag aatcaggtaa tgatcctcga
     8821 acggtagaag gttattacgg tgatggacag agagaagttg acttcatcaa acaattcact
     8881 gatggtctga tgggtattgg ctatggtcaa tggacagcag gtcgccatac tgcattagta
     8941 aactgggcga agaaaaactt tggtggcgct tggtggacga cagaagcgca attggatttt
     9001 atgttcaaag gtgatggtgg atttgttgct atattaaaag attatgcttt aaacgcttct
     9061 gatgatgttg tggcaaatgc cgtagcgttt cataagaatt gggaaatttc agcagattcc
     9121 gaatcagctg tagtggctgt acgtggtgga aatgcaaaag aaatctggaa gtacatgaaa
     9181 agcaaaggca tggacggagc taaagatgta agcaaaatca ataaaattgc cggaggaaca
     9241 agtaccggtg gtggatcaaa taattcatca agctcagcat caggaacagt agaagatgcc
     9301 tgtgcaacta cagatgatgc taattctggt tctggatcaa tgggtaaaga aggaaaaatt
     9361 aacggtaaaa aaggtcaagt aatagcgaat atgacttatg aagaagcttt gaaaaaatat
     9421 gggaaagcga tcacgctacc aaaattcaaa gaaccagatt ggtcaacttc accttttaac
     9481 ggaatatatc aaggtcagtg tacagagttg acgtgggctt atatgagcca attatacgat
     9541 gggaatcaac cgactactgg aaatggtggc tacgtatggg aaagctacaa atcggcagga
     9601 gcgaaaataa cgaacaaacc aatcgtaggt tatgggttta gtgcttcaga tggatggtta
     9661 tgggctgttg gcgatgcagg gcatactggt gtagttatag cggtgtttga agatgggtca
     9721 tttctatgtg ctaactttaa tgttccacca acccaagctc caacaagggg agtttcggta
     9781 actttgattg atggagtgga cggatcagat aatgtgcatt tctttagcgg tgtaggaaaa
     9841 gctaaatttg aaatcgaaaa ataaattgga atgaaggacg atatagatga atagtaaagt
     9901 gaaagataca attatcattt ctttgctttc tggtatgtta ttagcactgc ttgttggaat
     9961 cctattcttc aataagcaga atcaggacat agaagctgaa aaacacagtg tgcatattga
    10021 agataagctg acattcttta aagaggttaa accaaatgag gtggatggat tacggaaaga
    10081 tttagaaaat gatattcaag aaatgttgaa tggatcagtt caaaataagt ttgcagatca
    10141 atcaggtgaa gaatttttga gaaaactatt tattgtcaca ggtaaaccac caattactga
    10201 taagagtagt gagaaagaaa aaatcgagta ctataagcct tttactttta agctaacaaa
    10261 ctttggaatt aagacatcta gtagtgggtt gtatgaagca ttgaccgatt ttgaagttca
    10321 atacaatgga aaaaaagtgt atgataccac attaaccatc acactaaata atgataagaa
    10381 agtgactggg ggtgcttttt atgcaaaatc atgaaatcac tactgctaaa aaggcgattg
    10441 gaatactttc cataattgtg atcctttgtt gtgtggtact atttcaaaag aaaaatgctt
    10501 atgataataa tgtcaaagaa ttggaagacc ttaaaagtaa acaaatcgaa gtagtcaaga
    10561 ataaccagaa agtgaaagct atgcaggaag cccaatacaa agaattgggg aatgctgaaa
    10621 tcaaggaaca aagtaggaaa ttctttaatt tattttacaa ctggaattca tgggaaaaat
    10681 atacgaagaa tatgaaggaa atacaaaaag aatttcctca gatagtaaag tctagtcctg
    10741 ttgatatttc tggcacgatg gtaggcactg gtaatagtcc aatatctagc tatactgctg
    10801 attactatac aacaaatcaa aaaggtaaaa tggttgaatt gattacacag acaagaaata
    10861 ctccaacaac agcaacaaca acaatttggc gtggagtaac aagcatagga gaaaatcaat
    10921 tgttttactt ggagaattta gatagctatg taagagcgga gtaaaataaa aaaagagcta
    10981 acggtaccaa cgttagctct ttgtgctgac gatgataaca aaacctaccg aaaaaggatt
    11041 gtttcatcac aaacacaaac agaaaagaat tgataaaaaa cccgaacctc agaattttta
    11101 ttaatcaatt catttagatt ataataaatt gagctgaaca ttacaatacg tatatgttgt
    11161 aaaacgtaaa aaaagtcaaa agtaaatact ttcttaaaaa gtcaaataaa actattaaag
    11221 acaatatata tggtttattt gacataagaa aatggtctag atgataattg taatatagaa
    11281 ttgtaggctt aaataaagca taaaatacct tttatgataa ggtatagctc ttaattttaa
    11341 gcagttttta gatataagag tgaaagtaaa gtgggggaga ataataatgg cagatcgtaa
    11401 attagaaaag ttattagaag aaacgtggaa tccaaaagaa tttagtgaat tttttatgga
    11461 gaattttgaa actgacttag cagtgattgt taaagatgct cttagagagc aagggtatcc
    11521 tgaaactgca aactatataa acattaattt tactttgtat actgaaaata aaggaacttg
    11581 ggatttttgg gctactttag caaataagga gttaagtgat aaatcagaca caggaattcg
    11641 taactttttt gaatcaaaca gagatgatta tatgtatgct aaccatcaag ataaattgaa
    11701 ctttagagta gagtttgatg aaacacctga agaatttata gaaagacaac cgcctaaaga
    11761 aaatgtagca aaggtattgg aagatcgttg gaattcagat gaaatagttt caacaattag
    11821 cgaactaggc ggacaatatg aaccgctagt agaagcggtt agagaagagc taagactgaa
    11881 taagtttcct gatgttcaaa atattgatgt ttctcaaatt gaaataaacg tcaagatcac
    11941 gaataaactt gattatggta gttgggcaga tatagcatta gaaaaataca tctactcaac
    12001 gttaaaagag tttatcgaaa atagaatgga tatcatgtat ctacagcatc cacagtattt
    12061 aaactttggt gttgaaatag ctacacctct tgaagaatgg aaaatggagc aagggcttga
    12121 ctagcctttg ctccattttt ttaacagtaa agaaagtata aaaagttaag gactaaaaag
    12181 gctgcactac cagtcttttt agcccttttc attttgtttt tgaacaagta aaatattact
    12241 atgaaagcga atattctcga agcaatcttt tttgacgaac accataattg ggataatttt
    12301 gttgagaaac acgggcgaaa gattcggcta gtcgtcaaac gggaagtaaa caagtttaga
    12361 tattgtggag atattcgaaa aggataccga ctgtttgtct gcgaaggatg tcatcaaaca
    12421 aagtttgttc ccttgaaatg tcgtggaaaa ttctgtccaa catgtgcaac aggtgaaagc
    12481 caaaggtggt cagcggttgt ggcgaatgat atgtttaaag tgacacatcg gcacgtaatc
    12541 tttacgatcg atgaggggtt acgggaaatc tttatgatgg aaaactatcg agattcctta
    12601 ctgaaaggac taatggatga agccgcaaaa attattcttg cctattttga gaaaagaaag
    12661 gtgaaggcag gtgtagtagc tacaatccac acctttggat caaaactaga atttaatccg
    12721 catgttcata tggttgtcac tatgggtggc ttaacaagag atggaagttg ggaagactac
    12781 gattatttac ctttttctat gttaagaaaa tactggaaaa atgctgtatt gaaactaatt
    12841 agacgaacgt taggtgagtg ggacaagcaa agagttcaat caagattaca agctgcgtac
    12901 aagaacaatg gagaagggtt ttacgtaaat gctcctaaaa gaagcaggac aaatctaaag
    12961 tcactactcc aatacatcag tcgttatatg aagcgtggcc cgatcgcatt aagccgaatc
    13021 ttaatgtatg atggcgacac tgtcatgttc aactatatgg acaaacgaac gaatactaaa
    13081 gagacgatga ccatgagtat agacgaattc atagctgcac tgattcgcca tattcaaaat
    13141 aagaatttca aaacgattcg tcgctatgga atctatagtc gtagaatcaa aacattaatg
    13201 aaaaaggtca tgaaggagta tcagaaacga gtaaaaaagc tgtttatcaa tctgaatgag
    13261 gccttaagac caaaatcctg ggaagaaaga ataaccgaag aatttggtga aaatccctta
    13321 gaatgttctt gttgcggtga gtactatgaa ttcgttggga tgagtgtgcg caaaaatgga
    13381 aggttagtta ttaaatatgc gaaagataac catgcaaagc gatttatgcg agaggagaat
    13441 cgaaaaattg accaaaaaga attcaggaaa cagtatgaag aagaaaaaga aaaaactttt
    13501 aaagcaattc gctgggattg gggacgccaa cgtgaaattt atatgtctaa gttgtggtga
    13561 aaaagaatca atcccaatgg atgtagtaaa atttttagat gatgcagata ttgcaaatga
    13621 tccgaacaat cctccccaat ttacctgtga aaaatgtggg aaggagatgt atccggaata
    13681 ttatagaaat gcccttggaa tagagtttaa gatcagtgat cttcaataag aaggttaatc
    13741 atacaaaaag tccaccggag aatgaattct ccggtggttt tcttattttt attaagttaa
    13801 agaacggatt caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg
    13861 attactttgc catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg
    13921 acagtaccag gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt
    13981 acgtcaatca attgatgcat agggaaggta acgccataag tggttttaca agcttctgct
    14041 gcttcagtcg ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc
    14101 agaccttgtg tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga
    14161 ccgcattttg ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa
    14221 tagacagtgc cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct
    14281 ttctatttta tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt
    14341 agaactcttt tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct
    14401 tctcatcttt acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg
    14461 ctaaaaattc acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac
    14521 attgtgtttc attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt
    14581 caagaacatc taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg
    14641 tttggcatcc cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg
    14701 cagtcaagtg ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt
    14761 taaagcggac gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta
    14821 cacgtacata aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc
    14881 tcatttgtaa gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta
    14941 tttagtgaaa tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt
    15001 gtagcaatct gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt
    15061 cttgattctg tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc
    15121 tacagaaaga cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat
    15181 agacgcatga gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc
    15241 tgctaaagta aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa
    15301 agtaaaagaa atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc
    15361 atgatcgggt aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc
    15421 agctaccaca aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc
    15481 gcgctgaagt aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa
    15541 tccgatctga tcggctagat ctttatggtt agaagtcgct aaaccagcta ctacatcgct
    15601 gtgtccgcct agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg
    15661 tctttgaaaa taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg
    15721 agccagagtg gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc
    15781 aagataaagt gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt
    15841 atctatgatc gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt
    15901 tcctccatag acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac
    15961 ggcatgaatc ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc
    16021 agcaatcagt tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg
    16081 tttaggctgg ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac
    16141 agctcctgtt gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat
    16201 gttt
//
LOCUS       DAYYNG010000057        16091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000057 DAYYNG010000000
VERSION     DAYYNG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.057"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..698
                     /locus_tag="ACHBFA_001455"
     CDS             144..698
                     /locus_tag="ACHBFA_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9394954.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(871..1308)
                     /locus_tag="ACHBFA_001456"
     CDS             complement(871..1308)
                     /locus_tag="ACHBFA_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9394955.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1356..2303
                     /locus_tag="ACHBFA_001457"
                     /pseudo
     CDS             1356..2303
                     /locus_tag="ACHBFA_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2574..3009
                     /locus_tag="ACHBFA_001458"
                     /pseudo
     CDS             2574..3009
                     /locus_tag="ACHBFA_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3025..3858
                     /locus_tag="ACHBFA_001459"
     CDS             3025..3858
                     /locus_tag="ACHBFA_001459"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9394956.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4103..5128
                     /locus_tag="ACHBFA_001460"
     CDS             4103..5128
                     /locus_tag="ACHBFA_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9394957.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5266..5391
                     /locus_tag="ACHBFA_001461"
     CDS             5266..5391
                     /locus_tag="ACHBFA_001461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394958.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5416..6531
                     /locus_tag="ACHBFA_001462"
                     /pseudo
     CDS             5416..6531
                     /locus_tag="ACHBFA_001462"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6614..7510
                     /locus_tag="ACHBFA_001463"
                     /pseudo
     CDS             6614..7510
                     /locus_tag="ACHBFA_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7526..8350
                     /locus_tag="ACHBFA_001464"
     CDS             7526..8350
                     /locus_tag="ACHBFA_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394959.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8382..9692
                     /locus_tag="ACHBFA_001465"
     CDS             8382..9692
                     /locus_tag="ACHBFA_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM9394960.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9840..11099
                     /locus_tag="ACHBFA_001466"
     CDS             9840..11099
                     /locus_tag="ACHBFA_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9394961.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11109..11981
                     /locus_tag="ACHBFA_001467"
     CDS             11109..11981
                     /locus_tag="ACHBFA_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394962.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11993..12823
                     /locus_tag="ACHBFA_001468"
     CDS             11993..12823
                     /locus_tag="ACHBFA_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9394963.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12863..15046
                     /locus_tag="ACHBFA_001469"
     CDS             12863..15046
                     /locus_tag="ACHBFA_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9394964.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDKTVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15107..>16091
                     /locus_tag="ACHBFA_001470"
     CDS             15107..>16091
                     /locus_tag="ACHBFA_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9394965.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQ"
BASE COUNT         5447 a         2411 c         3159 g         5074 t
ORIGIN      
        1 ctgagttact gaaatgatat aaaatcataa actaaatccc ttgattttat tgactttcaa
       61 aagtttatat atataagtta atgattatgg taaaatatat acataatcat taactcagaa
      121 tatttttaag aagggaaaca atagtgagct ataatgtaca accgttaagg acaaatgaag
      181 agatacaaga ttttcagttt tggctaagaa gaacaagtaa tccggaaaga gatagttttt
      241 tatttttgtt tggaatcaat aatgggttac gtatgtcaga tattatagga ttaaaagttg
      301 gagatatacg aggcaaaagt aagcctatta ttgttgaaca taaaacaggt aagcgaaaac
      361 cgatttttat agataatttg agagaagaaa ttttattata tactgaaggt aaagaagaaa
      421 atgattggtt atttccgagt cggcaacaag gtcgacatat aacaagggat cgtgtctatc
      481 agatatatgc agatatagca gaaaaattag gacgagatga tataggaaca catacattaa
      541 gaaaaacttt tgggtatcac tactacaaga aaactagaga cattgctacc ttaatgttta
      601 tttttaatca tagctcgcaa gcaattacaa aaaggtacat tggtataact gaagatgaaa
      661 taggagcatc acttcgtgga tttaaactag gtgtataatt taaaaatttt tataaacagt
      721 aaagacaaag attaaaagct atttttatct agaacgattt tatttcataa agttcgcgga
      781 ataaaagctg gggttactgg tacttggtat aggttcgtgt tatataaact aagtaattaa
      841 ataacatttt taggttggaa aaggatgaaa ttaaaaaaag agaatcaata cggtctcaaa
      901 aacgcaaacg ctgcggaata agaaaaatac tgataactca acgtttagca gactatctaa
      961 tagggatagt ctgctttttt cgtttttgac catacttttg accttctgca aacttccttg
     1021 cttttcctct gtagaactca tgaaatctgc aaaacgaatt gccgtttcct ctgtctgctt
     1081 tttcgcgata tgggtgtata tatcatccat agtggtttta atatctgtat gtccaagtcg
     1141 aacatacact tctttgacag acgcaccaga ttcaaagagc agactacaat gggtatgacg
     1201 aaaaccatga agtgtgatat gttttaagtt attctcttta atgatgattt tcaaattgtg
     1261 atttacatag tctaaataaa ggggcttatt ttcttctgat gaaaacaaaa gttgttcacc
     1321 atcttttata ggtttgattc tcatttttag atattatgcc cgcacgtatt catcacattt
     1381 ctattgtgaa tcgctttatt cgaccgacat ttgactttta tcataatata ctcggtttaa
     1441 aattactaat gaaaacaatt aatcaagacg atcataccat gtatcacctg tttttttcag
     1501 ataatcacca ccgtgtggga actgaactga ctttttttga agtacaagaa gggaacaacc
     1561 agttttttgg aaccaatacg attgaacgaa cgatactaaa agttccttcc gaagcatcat
     1621 tgcatttttg ggaaatatat tttgaaacac aaggcgtttg ccattatgga atcgaatcat
     1681 tttctggacg acctatctta cgttttgaag ggccagatgc aacacgatta gctttagttc
     1741 ccttacgcga atttgaagat attgatgatt atttcccgta tgtcacagac cagatcccta
     1801 cagagcatgc cattttagga attgatgcca ttcaactacg cgtacaatat tccgatgcga
     1861 ctgaacgaga gttattgtcg gtaggctggt agcacaaaga atacgtcagt ttcttttcgg
     1921 gtcatcaacg agtgacaatc ttagaaaatc aagacactca gttctatcaa gaagttcaga
     1981 ttattcaaga tcgtgagaat ccagttgcca ttgaagggat tggtagcgtc catcatgttg
     2041 cgtttggcgt agaaaataaa tctgatttac aacgcatcga caaacaatta caagagcgca
     2101 attttataaa ttcaggaata aaagatcgtg aattttttgt atcactttat tatcgtgacc
     2161 caaatcaatt attgattgaa attgctacag gggaaggaaa cctagatgca aaagcgtatg
     2221 aaaatcaaag tccacgcttt gaagaaattc ctttattttt accgcagtac ttaaatttca
     2281 ttcgtgaaca agtggagcaa taattagcta atcaaatcca tgacgaagag taagaggttg
     2341 actcataagt caaaaaataa agaaaaacgc atcaaatcca ttctaaagaa tgttgatttg
     2401 atgcggttcc tatttttata aactatcatt ttcgactaat aaaactactt atgagtcagc
     2461 ctctcttttt ttcttgattt ttatacctct ttctaggtaa actaaacatc tgccaaaaat
     2521 accgtgtctc attgaaacat tctcaactat ttcaaaataa aggggagtac caaatgaacg
     2581 agtatattga aattatccac gcaagagaaa caacttaaaa atgtcagtct caaaattccc
     2641 aaaaataaaa ttagtatttt taccagcgta tctggttcag gaaaatcatc gattgttttt
     2701 gatactatta cccaagaagc tggtcgtcaa ctgaatgaca cctatgatag ttttacccgt
     2761 ttatttttac ctaaatacag tcgtccagag gttgatgaga ttgataatct ttctacggcg
     2821 attgtggtca atcaaaagaa gattggtggc agtgcgcgct caacgttagg gacgattact
     2881 aacatcaatg cgttatttcg tgtgttgttt tcatgttttg caacacctag tttaggatat
     2941 gcaaatgctt attcatttaa tgatcctagt ggaatgtgtt ctgtctgtca gggaatcggt
     3001 aagacgattg catttttaat aggattgctt tatttttcgg ctattgataa tgctgatttt
     3061 gtattttctg atccatttac cgcagaagtt ttgtctggaa ttgaggaaca ggcaaagaat
     3121 catggttatt ttgtattggt gcataatgtt aattcagcag aagatgttaa aactattcag
     3181 agaaattggc gttttgaggg gttcattgtt gttggtgtac gtgtaaaaga atttttggaa
     3241 ttaaataacg tttttgatgg tcctgttgtt tatattgata cgcatctttc aaaagagaca
     3301 ttaagagaac ttgaacaaca aatgaatcgt ttatttgtaa atacaaatga ttaccaagct
     3361 agtacattgg cagtggaaca tttgattcat tatggtcatg agaaaatagc gtttctttct
     3421 tttgactaca ttgtaggtga accaagtgtt attcaagaac gacatcaagc ttatatcgat
     3481 acattaaaga aatatcattt aacctttaaa tcgcattgga tatataacaa ctctgaattt
     3541 gagcgaattt atcaagaatt agctgaattt actgcaattg ttgttactgg agattattta
     3601 gcggctaaat tagtaaaatt tttaaaaaat aagaataaac gagtgatgga acaattatcg
     3661 attattggat ttgatgatat tacttttgca gaattaatgg atcctgcgtt gacaacaata
     3721 cgactaaatc caggtaacaa gggggcgatt gcttttcacc aactgattgg actggccaaa
     3781 caagaagaaa taaaaaatca agtactacta ttagagggag aattgatttc tcgagactct
     3841 gttcacaaaa tcaaataaaa aagctctgtg aaataagtat agttaacaat aaaatatccg
     3901 aacgatagag agaatgaact gtgatctagc agttcctcgt cgcaaattca ctagaattag
     3961 cattatttac tctaaattgt tcggatattt tttcttgttt ttaattatga ttacagtgcc
     4021 tatttttaac gaaatttgct tattttcaaa atgtatgatt atatgttagt aaacactttc
     4081 aaatatgaaa agaggagcta ttatggtagg aattagggat gttgcaaaac gggcgggtgt
     4141 gtccattgca acagtatcgc gcgtgctaaa tgaggatccg acactttcag taaccgatga
     4201 aaccaaacga aagattgcag aatcggttaa tttttatcat tatgtcaaaa aaagtcccgt
     4261 tgcaaaaaat ttaacaaata ttgcattaat aacgacagtt tctgaagtag atgagttgga
     4321 agatccctat tttcgaacga ttcgcagagg gattcaagtc gaagcagaac aaagcaaagt
     4381 caatttgaag aaaattattc gtttgtcaga aacaccgttg gagcaagacg atttgctaaa
     4441 atgtcaaggt gtgttgatta ttgggcaggt gctatcttct gtgattgaag aagtatcagc
     4501 aattaattcg aatgtcgtag tgattgatga ccctagtatt gaagctgaag tagatgcagt
     4561 ttatactgat ttaacaaaag caacgaatca gcatttagat cgattgtata aaaaaggaca
     4621 ccgaaacatt gcgtttatcg gaggaggacg tgttcgttta gatagccaag gaaataaaca
     4681 cgtgtcagat gatgattatc gaaaaatagc ctatgagacg tggatgaaac aaaaaggatt
     4741 agaaaaatat agtcaaattt atttagaagg ctggaccaca cttgacggaa tgcaggcagc
     4801 agatttactt ttgaaaaaac atgctaaaga taagttgccg actgctatca tggtaggaaa
     4861 tgatcctatt gctgtggggg tttatcgttc acttcaaaaa aatggtctac atattccaga
     4921 agatatttcg attgtgagtt ttgataatat tgaagttgct gaatttttaa cacctgcatt
     4981 aaatacggtg aatgtgaata cagaagaaat tggaaaacta gcagttcgca tggttaatga
     5041 acgaataaaa gaagttagaa atgtttcgat caaagtgatt gtttctaata atattattgt
     5101 tagagaaagt gaaaaaaata tcgattaaaa aagtaaacat ccaaaaaagg gtgtttactt
     5161 ttttgtttac taatattttt agtaaaaaat ctcgacaaaa cattaagtaa acagttatgg
     5221 tgttgttaac aaaaagaaaa cgcattcaaa acaggaggaa ttaagatgaa acatttcaaa
     5281 aaaatagctt taacagggat agctgtttta actgggacat tactcgcagc atgtggtggg
     5341 ggagatagta attctaaaga cgggcaagta actttaagct atgcgagttg agatactgac
     5401 caagctccag gactaagaaa aatgttagat gaatttgaaa aacaaaatcc tgatatcaaa
     5461 gtagaaatgg agacaacgcc ttgggatcaa tattgggtta aattagaagc tgcaacaacg
     5521 ggaggtaata tgccagatgt agtaaccatg cactctagcg aatcctataa atatatgtca
     5581 cagaatgctt tgatgggact tgatgatgtt atcaaagaaa atgatattga catgggaaac
     5641 tttactgaag gaattgctga tttctatacg cttgaagacg aactatacgc aatccctaaa
     5701 gatgcttctg tagtggggct atggtacaac aaagaattat ttgataatgc aggtgtcgat
     5761 tatccagatg aaacatggac atgggaaaca ctcagacaag ctgcaattga tttaacagat
     5821 gaatcaaaag gcatctatgg ttttgcatca catattggaa cagagactgg ctactggcca
     5881 tttatttatc aaaatggggg agaagtattt gccgatgaca atacaaaatc aggtttgaat
     5941 accccagaag cacaagaagc tttagctttt tatcgagata ttgcacttgt tgatcatgct
     6001 tcaccaacaa ttacagaatt acaagatcct gataagttaa gtcgttttca atcaggtaga
     6061 ttagcaatga tgattgaagg aaactggttt gcaccgggat ttttgaaaaa tgactatcta
     6121 gttgaaaatg ttgcttctgc cgtgttacct aagggaaaag ttaatgcaac aattacaaat
     6181 ggacttggtt gggctgccag tgcgagtaca aaatacccag aagaagtgaa aaaactaatg
     6241 gcatttttag ctagtgagga cgccaataaa atccaagcgg aaacgggggc atcaatccca
     6301 gcactaaaag gctttgaaga cgagtgggct gcttcttcag atacaattga tttgagtgct
     6361 tttggaaaaa tgttggagta tggagtccct cgtccataca ataaagatgg attacgagct
     6421 gaacaagcag aagttcaaat tattaaccgt attctgtctg gtgaagtaac tgttgaagat
     6481 ggaaccgctc aaattgaaaa agaagtcaat gcgatattga gcggaaatta aattagttaa
     6541 ttatacaata tacaggcgaa aggattctat ttcgtctgta tattaatgga tgaataccaa
     6601 aaagggggat attatgaaaa atcgaatttt tggaaaatat cgtaaagatc aagtgatatg
     6661 gggctatatt tttatttttc caacggcatt aggattaatt ttattaaata tatggccagc
     6721 aattcaaaca gcaattttaa gttttcaaaa aacggtaggg ttcggtgata ctgaatgggt
     6781 cggattaaga aattaccaaa aaatgtttgc agacggtgaa gtttttcagt ctttattaaa
     6841 cacattaatt tatgcagtgg tttcgatacc tagtattgtt gtattatcct tgttggtggc
     6901 agttttaatg aatagaaaag ttaaaggatt gtcgatttat cggacaattt attttctacc
     6961 agtcgttgct gcaccatccg cagtcgcaat gatttggcgc tggatgttca ataatgatta
     7021 tggactgatt aataacatgt tggctaaaat aggtctcgat ggaccagcat ggttaacgga
     7081 tccatcgatt gcgatttatt ctattatcat tgtaggagtt tggagtgcta ttggttataa
     7141 catggtgtta ttactagccg gcttataaga aattcctaaa gattattatg aagcagcgga
     7201 aattgatggt gctagtccaa tccagcagtt tttcaacatt acgttaccat tagttacacc
     7261 aacattattt tttgtagttg taacaacagt tatcaatgct ttccaagtat ttgatgttat
     7321 ttttatgatg attactccta atagtaccgc catgtttaaa tcacaatcat tagcctattt
     7381 attctataaa cattcattta ttttaaatga caagggatat ggttcggcaa tcgttatgtt
     7441 ccttttagtg attatattaa ttattactgc aattcaaatg aagttgcaga aaaagtgggt
     7501 aaactattaa ggaggctatc aaattatgaa aaataaattt ttttcaaatg cgccaatcca
     7561 tatcatgctt attttaggtg gtattgcaat ggcattacct tttatttgga tgattttaac
     7621 ttcagggaaa acgttggttg aatctaccca aattccacct acaattttcc ctgagaaatt
     7681 tcagctaagt aactatcaac aagtacttga tttattacca ttcatggctt tttatatcaa
     7741 tacattttta atggttgttg gacgagtgat tggctcggtt ttattttcag caatggctgc
     7801 atatgcttgt gcgcgtctaa gttttccagg gaaaaatatc cttttcagct tagtacttat
     7861 tcaaatgatg attcccggtt ccatttttat tactccacag tatttattag cacaaaaatt
     7921 gggtatttta aatacaattc cagcattaat attaccaggg gtagttagtg cgtttggtac
     7981 gtttttatta agacaatttt tcttaggttt acctgatgaa ttggaggaag cggcctattt
     8041 agaaggtgcc aatatttgga cgatcttttt caaaattatg ttgccgcttg ctaaatcagg
     8101 attggttgca cttggaatat tcacagcgtt gtttgcattt aaagatttaa tgtggccatt
     8161 gattgtcaat acttcaattg ataagatgcc tttagctccg ggattagcaa acttacaagg
     8221 acaatatgcc acgaacttcc cacaattaat ggcaggttcg ttattagcaa tttggccaat
     8281 agttgtgata ttcatcgttt tccaaagaaa attcattgaa ggaattgcta caacaggtag
     8341 taaattataa aactattaat tagtgattgg agagtaagta catgacaaaa attacattta
     8401 ttggtgcagg aagtactatt tttgcaaaaa acgtattagg cgatgcgatg ttaactccaa
     8461 gtctacaaga tgcacatatt gcattgtacg atattgatga aaaacgacta aaagaatcaa
     8521 atttaatgct taaaaatatt aataaaaatt cgaatgaaaa tcgtgctgat attgtttctt
     8581 attatgatcg caaagaagcg ttaagagagg ctgattttgt tatcaatgct attcaagtgg
     8641 gtggttataa accttcaaca gtgattgatt ttgatattcc taaaaaatat gggttgcgac
     8701 aaactattgg tgacaccatg gggattggtg ggatttttag aaccctacgg acactaccag
     8761 taatgttaga ctttgctaaa gaaatggaag aagtttgtcc aaatgcttgg ttattgaact
     8821 atacaaatcc gatggcgatg ttaaccatgg gtgttttaaa agcgacgagt attaagactg
     8881 ttggtttatg tcatagcgtt caagtatgcg taccagaatt gtttgagcat ttaggaatag
     8941 ccgatcaata tgacttaaat gactttcaat ggaaaattgc aggcattaat catatggcat
     9001 tcttgttgga aattacaaaa aatggggaag atttctatcc aacaattcgt cagttagctg
     9061 cagaaaaacc aaatccacat ccagattctg tacgttttga attgttgaaa cgttttggtt
     9121 actatattac agaatcaagt gaacacaatg cagaatacca tccatacttt attaaatcaa
     9181 aatacccaga attgattgac gaattaggga ttccgttgga tgaatactta cgtcgttgtg
     9241 aacaacaaat taatgattgg gaaagcatgc gagaggacat tgtaaataat ggggagttaa
     9301 cacatacgag aagccgtgaa tatgcttcat atatcatgga ttctattacg acaggaactc
     9361 cgtcagtgat tgcaggaaat gtcttaaata aaggcttaat cacaaactta ccgacagatg
     9421 tttgtgtgga agtaccttgt ttagtcgata aaaatggcgt gcaaccaacc tatgtcggtg
     9481 aattgccaac acaattagct gctttaaatc ggacgaatat caatgttcaa gaactgacag
     9541 tagaagccgc tttaacacta gataaatcaa aaatttatca agcagcgtat ttagatccac
     9601 atctatcatc tgaattgtct ttaacagata tcaaaaattt agtggatgat ttaatcgaag
     9661 cacatgggga ctatttacct gaatataatt aaaaagaagc tgaaagagta aggaattttc
     9721 aattccttac tcttttctta gttaaattta gtaaaaatat tgacaaaaca cttagtaaac
     9781 agttacatta gtgttaacaa aaagaaaacg cattcaaatt gtataaggag gagcataaaa
     9841 tgaagtggtc taaaaaaatt attttagctt gtatagcact aggagcaacg atctctttga
     9901 ctgcttgtgg aaaaaatcag gaagatgcaa gcggtaaggt agaaatagag ttttttaatc
     9961 aaaaaaaaga aatgactcaa acgattcagg agattgctaa agattttgaa gcaaaaaacc
    10021 ctgatattca tgtaaaagtt gtggatgtac cgaatgctgg tgaagtaatt aaaacacgta
    10081 tgcttgcagg cgatgtacca gatgtcatta atctttatcc tcaaagtata gaattgaaag
    10141 aatgggcgaa agctggttat ttagaggact taaccgaaga accgtattta gagaatatca
    10201 aaaatggata tgcacaacgt tttgctattg aggatcgtgt atatagcata ccattaaccg
    10261 ctaatgtcta tggtttttac tataacaaaa ctgcttttga ggaaatgggg attcaagcac
    10321 cagaaacttg ggcagaattt gaaaaaatag tagctgagat taaagatcaa aacaaagtac
    10381 cgtttgcgat tgcaggagca gaaggctgga ctttaaatgg ttatcaccaa ctagctatag
    10441 cgactgctgc tggtggtgaa gaggaagcga acaatgtttg gcgtttctca gaagttaatg
    10501 gaattaatgc tgaaagcaaa gaaatgcaaa aagattttaa tcgattagat ttgttaagag
    10561 aaccaaaagc tttgcaaaac aactggcaag gtgccggtta taacgataca gtagttactt
    10621 ttacaaaagg tgatgcgttg attatgccga atggttcatg ggcaatgccg atgattcatt
    10681 cacaaaatcc agattttgaa gtaggaactt ttccttttcc agcagatgaa gcaggtcaaa
    10741 gtctaacgat tggcgcagga gacctagcct tatcagtttc tgcaacttct caacataaag
    10801 aagaagccaa acgttttgta gaatatatga caacacctga agcgatgcag aaatattatg
    10861 atgtagatgg tgcaccgtgt gctgttgagg gcgttatcga gaatactcag gattcaccat
    10921 taagtggttt aactgaactt gcttttactg atcgtcattt agtttggtta gccaaagatt
    10981 ggaatagtga aaatgatttt tataccttaa caacaaatta tttacacaat ggcaatcaaa
    11041 aagccatgat taacgcatta aatgctttct ttaatccaat gaaagctgac ggaaggtagg
    11101 aaaataaaat gaaaataaaa gaaattttaa atcgttactg ggcaatcatt tttgtagcaa
    11161 ttccaattat tttgcagatg atttttttct actttccaat ggttcaaggt gctttttaca
    11221 gtttaactaa ttggactggt ctgacttata attttgattt tgtgggtttg aacaattata
    11281 agattctttt ttctgatccg aagtttatca aatcgattgg cttcacttta attgtgacgc
    11341 tttgcatgat tgctggacaa attggttttg gtattttgat tgcgcgggcg ttgaacgcaa
    11401 atattaaagg gaaaacattc tttagagcat ggttcttttt tccagcggtc ttatcgggcg
    11461 tggttgtttc tttagtattt aaacaaattt ttaactatgg tttaccaaaa attggtagta
    11521 tgttaggcat tgaattttta caaaccagtc ttttaggaac aacatgggga gcgatttttg
    11581 cagtaatttt tgttttactt tggcaaggaa ttgccatgcc agtcatcatt ttcttagcgg
    11641 gttttcaaag tattccacaa gaaattattg aagctgctag aatggatggg gcgaatgata
    11701 agcaaatttt ttggaatatt gaaatcacct acttattacc aagtatttca atggtattta
    11761 ttatggcatt aaaaggtggc ttaacagcat ttgatcaaat ttttgcttta actggtggtg
    11821 gaccaagcga tgccacaaca tctttggggt tacttgttta taattatgct tttaaaaaca
    11881 atcagtttgg ttatgcgaat gctattgctt tagtcttatt tatcttaatt gggattgttt
    11941 cactggcaca attaaaatta tcgaaaaaat ttgaaatcta ggaggtttcc caatgaaaac
    12001 atcaaaagaa aaaaatattt ctaaatactt gcttttatta ttagggggct tcatgatttt
    12061 agttccctta gtttcaacgc tattcggttc atttaaaaca acacccgata ttatgaataa
    12121 tttctttcgt tttcctaatc cagcgacttt ggaaaatttc cgaagattgt tagcggatgg
    12181 aattggtcat tacttttgga attcggcaat catcacaatc attgcagtag ttgctgtgac
    12241 agcaattatt cctatggctg cttattcact agcaagaaaa atgactcgtg ggaaagcata
    12301 cggattgatg tatttatttt taattttagg aatttttgtt cctttccaag taattatgat
    12361 tccgattacc gttatgatga gtaaaattgg tttagcaaat atgtggggat taatcttact
    12421 ctatctcact tatgccgtac cacagacact atttttatat gttggtttta ttaaactaaa
    12481 tgtgcctatc agtctggatg aggctgctga aattgatggg gctagtcact ttacaactta
    12541 tttcaaaatt gttttcccaa tgttaaaacc aatgcatgcg acgactatta ttcttaatgc
    12601 tctatggttt tggaatgatt tcatgctacc attattaatt ttaaatcgtg attctaatat
    12661 gtggacacta ccattgtttc aatataatta tacgggacaa tattttaacg actatggtcc
    12721 aagttttgct tcctatacag taggaattgt cacgattaca ctagtttatc ttgctttcca
    12781 aagaagtatt atagctggaa tgagcaatgg agcagtgaaa taaccaataa attattctca
    12841 ttataaagga gttcataaac ttatgattat atttgacaat cacacacaaa catttcattt
    12901 acaaaataaa aaaattagct atctattagg aatcgaagaa aataattatt taacgcatct
    12961 ttattttgga aaaaagatta cgcactattc tggtgggtat cgctaccccc gaacagatcg
    13021 ttccttctca cccaacaatg caaatgcgca agatcgttta ttatctttag acacattacc
    13081 attagaattt ccaggatatg gacatggtga ttttagagaa ccggcgcata atattaaatt
    13141 aggaaacggt agtcgtgtaa atgattttcg ttatgatagc tacgaaattc aaaaaggaaa
    13201 gcccagctta gcgggattgc cagccttata tacggaagat gatgatgaag ctgaaacgct
    13261 gattattcaa ttgaaagacg cgattagtgg attgacttta cagttgttct acacaattta
    13321 tcaaaatcac agtgtaatta ttcgttctac gcaattagta aataatagtg ataaaacagt
    13381 ggagattaac cgcttagcta gtcaatcaat tgattttcca aatcgtccac tcgagttaat
    13441 tcatttaaat ggtacctggg caagagaacg tcaaatgact agagaaaaaa ttcatacagg
    13501 gataaaagta ttagatagta aacgcggatc aagcagccat caacaaaatc cgtttgttgc
    13561 tttagtagat gccaaaacag atgaatttca aggagaggca tatggattta atttagttta
    13621 tagcggtaat cacgaaacgg tgattcaaaa agatccattt gaccaaacac gggtcattat
    13681 gggaattaat tcatttaatt ttggttggca actaaaaact ggcgactcat ttcaaacgcc
    13741 agaagtcgtg atggtttata gtaatgaagg tctgaatacg ttatcacaga cgtatcatga
    13801 attgtataat caccgtttaa ttcgtggaaa ctatcgttta aaagaacgtc ccacgttaat
    13861 caacaactgg gaagcaacgt attttgattt taacgaagaa aaaatcatga atattgtcga
    13921 tgatgcgaag aacttgggca ttgaaatgtt tgttttagat gatggttggt ttggcggtcg
    13981 tgaaaatgac ttcacttcat taggtgattg ggttgaaaca gaaggcaaac ttgcttgtgg
    14041 tttggaaaat ttagccaagc aagtgcatga taacgggatg aaatttggga tttggtttga
    14101 accggaaatg atttctgaag atagtgaact atttcgagca catccagatt gggcattggg
    14161 tattccaggt cgtgaccgct caattagtcg aaatcaatat gttcttgatt tttcaagaca
    14221 agatgttcgt gataatatct atcaacaaat gacggcgatt tttgatcgtg taccgattga
    14281 ttatgtgaag tgggatatga atcgaaatat gacagaagtc tattctgtct tgttggactc
    14341 aacgatgcaa ggtgaagtgg ctcatcgtta tatgttaggg ttatatgaat ttttagaaaa
    14401 gttaacaacg aattatccaa ctattttatt tgaaagttgt tcgggtggag gcggtcgttt
    14461 tgatgcaggt ttcttgtatt acatgccaca aacttggacg agcgacaata ccgatgccgt
    14521 ggctcgtctg aaaattcaat atggaaccag cttagtgtat ccaatttcaa gtatgggtgc
    14581 gcatgtttca gcagtcccaa atcatcaaac aggaagagaa acgagtttaa ctattcgtgg
    14641 caatgctgcg atggctggtg tttttggtta tgagctagat ctaacgcaat taacttctga
    14701 agaaaaagca gaaattaaag aacaagttgc cttttataaa aagcatcgcc aacttttgca
    14761 atatggaact ttttatcgct tggaaagtcc gtttgaaggc aatgatactg catggatgtt
    14821 tgtctcaaaa gaccaaacag aagccatggt cttttatttc cgagtattag cagaagctgc
    14881 gcatccatta gtcacattga aattagcagg attagcagaa gaagcaactt atcaaatgga
    14941 agatgtaaca tttgctggcg atgaattgat gaatctcggt ttttatattg actcagaact
    15001 acatggtgat tatgcgacac aacgctttta tttgaaacag atatagtagc ttgaatttac
    15061 aatataagtg caacaccaaa agagactcat tccaaacgaa gaaaatatgt tttatttaca
    15121 cgcatttact aaaaagcagc ctgatttgaa ttgggaaaat gaagaagtac gtgaagaaat
    15181 ctatgcaatg attaactatt ggttagataa aggactaggc ggatttagaa tcgatgcgat
    15241 attaaacatc aaaaaaaaac tagagtatgg catctttgaa tctgatggag aagatgggtt
    15301 agccttcatt gggccctgga ttcttaatca gtcgggcatt gaagtgtggt taaaagaaat
    15361 gagagagcgg acatttaaac cacataatag tatgacggta gcagaagccg atgttcctaa
    15421 tgaacgttta gatgaatata ttggtgaaga tggctattat tcgatggttt ttgattttag
    15481 ttatacggat attgatgtac ctgaaactgg tgaatggttt aaagatagcc aatggacatg
    15541 gacggatatg cgtaccaata tttttacgaa tcaattagtt acgcaagata aaggttgggg
    15601 cgccttatat ttagaaaacc acgatcaacc acgttcaatt aataaatata ttccagaaga
    15661 atggatcaat gattatagta aaaaaatgtt ggcgacgctt tttatgttac taagaggtac
    15721 cccttttatt tatcaaggac aagaactagg aatgaccaac atagaaatgg catcattaga
    15781 agattttgat gatgtagcta cgcattctca gtataaacgg gcattagaat atggattggc
    15841 accagagcaa gcattgaaag ctgtcaataa acgaagtcgg gataattctc gcacacctat
    15901 gcaatggaat acgcaaaaaa atgccagatt ctcaacttct gataatgtat ggttaaaagt
    15961 aaatcctaat tatccagaac tcaatgcaga agctcagtta accaatcaac agtcggtatt
    16021 caattattat catcaattaa ttaaattaag aaaaaatagt gtatacaaag acgtgctaat
    16081 ctacggacaa t
//
LOCUS       DAYYNG010000058        15974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000058 DAYYNG010000000
VERSION     DAYYNG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.058"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1168)
                     /gene="queG"
                     /locus_tag="ACHBFA_001471"
     CDS             complement(17..1168)
                     /gene="queG"
                     /locus_tag="ACHBFA_001471"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM9394966.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(1187..1939)
                     /locus_tag="ACHBFA_001472"
     CDS             complement(1187..1939)
                     /locus_tag="ACHBFA_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394967.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIHFVQLKKEKLYYGLYSR"
     gene            complement(2079..3029)
                     /locus_tag="ACHBFA_001473"
     CDS             complement(2079..3029)
                     /locus_tag="ACHBFA_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9394968.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            complement(3030..3869)
                     /locus_tag="ACHBFA_001474"
     CDS             complement(3030..3869)
                     /locus_tag="ACHBFA_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9394969.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(3866..4612)
                     /locus_tag="ACHBFA_001475"
     CDS             complement(3866..4612)
                     /locus_tag="ACHBFA_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9394970.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            4850..6220
                     /locus_tag="ACHBFA_001476"
     CDS             4850..6220
                     /locus_tag="ACHBFA_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9394971.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(6275..7171)
                     /locus_tag="ACHBFA_001477"
     CDS             complement(6275..7171)
                     /locus_tag="ACHBFA_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9394972.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7285..7455)
                     /locus_tag="ACHBFA_001478"
     CDS             complement(7285..7455)
                     /locus_tag="ACHBFA_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394973.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(7452..8129)
                     /locus_tag="ACHBFA_001479"
     CDS             complement(7452..8129)
                     /locus_tag="ACHBFA_001479"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9394974.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(8220..9125)
                     /locus_tag="ACHBFA_001480"
     CDS             complement(8220..9125)
                     /locus_tag="ACHBFA_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM9394975.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9300..10754
                     /locus_tag="ACHBFA_001481"
     CDS             9300..10754
                     /locus_tag="ACHBFA_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM9394976.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            10770..11801
                     /locus_tag="ACHBFA_001482"
     CDS             10770..11801
                     /locus_tag="ACHBFA_001482"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9394977.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            11808..12995
                     /locus_tag="ACHBFA_001483"
     CDS             11808..12995
                     /locus_tag="ACHBFA_001483"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9394978.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13151..14533
                     /locus_tag="ACHBFA_001484"
     CDS             13151..14533
                     /locus_tag="ACHBFA_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9394979.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14505..14891
                     /locus_tag="ACHBFA_001485"
     CDS             14505..14891
                     /locus_tag="ACHBFA_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394980.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(15091..15759)
                     /locus_tag="ACHBFA_001486"
     CDS             complement(15091..15759)
                     /locus_tag="ACHBFA_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9394981.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         4571 a         3221 c         2998 g         5184 t
ORIGIN      
        1 aatgttaact atttttttat tttaactttc tccgcaataa tttctgggca tgttccaatt
       61 cgtttataac gacttcttct gtttctttag ctaatatttc atctaataca tcaagccatt
      121 tttccggttc ttttgttcct agacgtccaa tagcccaagc tgctgttcct ctgatgacag
      181 ggcggacatc tgttaaacat tctgaaatct caggcaatgc cgttcgatca cctagattag
      241 ctaaagcaat caatgcgttt ctctgcaaag gtttcttccc acgccatgaa ccagctaaat
      301 gcccaaattg ctgtttgaag tctttattag acatcgaaag cattggcttt aattttggat
      361 atacttcgtc tacttttggt tccatctctt catgaaaatg aaaatctttt ccttgattgt
      421 aagggcagac taactggcag atatcacatc cataaatgac atttcgcatt tttttacgat
      481 attcttccgg catcatccct tttgtctgag tttgataaga caaacatcgc tgggcattca
      541 ttcttccgtc accaagcaat gcttttgtcg gacaagcagt cacgcagcgc atacagtcac
      601 cgcaaccaaa ttctcctggt ttatcaggct caaatacaat attagtaacg atctcaccaa
      661 gatagacaaa tgaaccgaac tcttcagtga tcaacaatcc gtttcgtcca ataaatccta
      721 aaccagctcg ctgtgctaca gcgacatcga tcagttctcc agtatctact tgcggagcta
      781 gacgccattc ctcttctttt tgccattttt ctgctcttgt ttggatgaat tcgatcaatc
      841 ggttcaaacg gtctctgagt atatcatggt aatcgatccc ccaagaagca cgagcaaact
      901 gcccgcgttt ttcatcccta ggcacttctt catggatcct agtaggatac gcaagtgcaa
      961 tggaaatgat cgatttcggc tgatcaaatg tcatttctgg ataaagacgc tcatcgatat
     1021 ttggatgttc aaagccagaa gtatggccag ctgctttttg ttcttccaac gattctttta
     1081 atgaatcaaa aggttccgcc gtggtgaacc cgattttgtc aattcctaat cgtttgcttt
     1141 cttgtatgat ttcttctttt aatgtcatgg tcttcctcgc ttactgttaa cggctgtata
     1201 acccataata tagtttttct tttttcagct ggacaaaatg gatctgctga tcggcaatct
     1261 tcttttcttc catccatttt tttcttgcag caaatacttc ccaataattt gttgaaatta
     1321 attttctttt gttatctaat gcattaaaaa aattcaatga aactggtttt gtacttaaca
     1381 gttcatggag ccttacctct tcccatggcg aaaactcaga cagatcgcgg gaatacaata
     1441 cttcttcata gagatttttt tgctgtgtgt acgtgaatgt ttgcgggatt ttcccaggcc
     1501 tggcaatttc tactagacga ttataaaaca tattcattaa tttgctgttg gcaataaatt
     1561 gacttttata ataggccgac tggatctttt tgattactcc ttctgctttg atgactgaaa
     1621 ataatgcagg ccatgctttg acggttattt cgtcgttttg tttaactaat acgccaggtg
     1681 tgaagccgac tggagaaagg aacacaaaat aaccgatacc ggtattttca tctaaaggct
     1741 gaaattcatc aaacaaccat ttgcctatgt acagctcttc atctggttct gttgtctcat
     1801 tttgatttgc tatgaataat ttagctaatt ctgcttcaag aaattgaagg tcttgatcct
     1861 tgtcggtatt ttcctgctgg ctgatcaatc gaaagacttc ttctttaatc gtatatttat
     1921 ctttacgtgt aaaattcata gccacacctt ccttccttac tctaactata acggaatttc
     1981 ttcaaaaaag gtatctgtac gccgaaaaac agcataattt ttttcatatc tcttataaaa
     2041 aatgcaaggc ctgctcctca agctagacct tgcacttttc agctttcttt tatttgtgac
     2101 aaaccttcat ggatcgtttc aatattccat ttcatcatct gatagtaaga atctccttgt
     2161 tctccttctt ttgctatgga atcagtgaac agtttatcat aaattttcaa acccgtttct
     2221 tttgctaccc gttccatgct tcttgagtct acactggttt ccacgaataa aaccggtact
     2281 tcttttgctc ttatctgatc aatgatcgct ttcatttgat caggcgtacc ttgactttct
     2341 gtattgatct cccatatata agctgctggc aaatcatatg ctcgtgaaaa atacttgaat
     2401 gccccttcac ttgttactaa taattttttg ttctcttcga tagaagcaaa actttccttc
     2461 gcttgtgtat ctaattcttt tagtttttcc acatatgctt ctgcattttt tttatagagt
     2521 gtcgcatttt ctgcatcttt ttcagagaat atacgtgcga tttcctccac atattttatt
     2581 ccgttagata ggtctagcca agcatgggga tctgcttttg tatgttcttc accgctagtt
     2641 aaataaagag gttctacatt tttgctaact gcaaaataat cttttccttc ttctttttta
     2701 gccgtttcca tcaagttatc gaaccagctg ttacctgttt caagattcaa accgttgtat
     2761 aaaataacat ccgcatcgct tgcctttttg atgtcttctg gtagtacttc atattcatgc
     2821 ggatctgttc cgactggtac gatactgtgg atatctattt gatctgttcc gacctctttt
     2881 gccatgtccg ccaagatcga attggtagcc acgactgaca acttttcttt acttccttca
     2941 tccgcttggt cagaagcctg ctttccgcaa ccggctaata ccaagaaact aactgccaag
     3001 attaaaaaga gtgatttttt tgttttcatc tatctgcttc ctttctgaaa taaaattcct
     3061 tttgttggag aaaataaaaa agcaaggata aaaaatgcgg tacatgctaa cacgatcgta
     3121 gctcctgatg gccaattgta actataactg aaaaacacac cgacaactgc acttagcata
     3181 ccgattcctg cagctgtaat gatcattttc agtaaatggt tcgttagcaa gtaagccgtg
     3241 gcggctggtg tgatcaacat ggcaatcacc aagattgttc ctactgtctg taaactgact
     3301 acagccacta atgtcaaaaa gaacatcaaa agataatgga tcagcgatgt gttcagccca
     3361 taagcttttg ccattgtcgg atcgaatgat gtgatcttca attctttata aaagaaaaag
     3421 acaaatatca agacgattcc actaacgagt gctgtcagca acaaatcgct ttccctaaca
     3481 gctaagacat tcccaaataa aatatgatac aaatctgtcg cactctgggc atatgaaatc
     3541 aagataatcc ctaatgcaaa aaaggagcta aagacaattc caatcgctgt atcatttttc
     3601 aatcggctcc gctgagtcac gaaaccaatc actgctgctg ttagcatccc aaaagcagtg
     3661 gctccaaaga tgtaactaaa accaaacata taagaaatgg ctacacctgg aagaactgcg
     3721 tgagagatag cgtctcccat caaagacatc cctcgtaaaa taataaaaga accgatgact
     3781 ccagaagcaa gtcctacaag catggaagtg accaaagcgt attgcaggaa ttgataattc
     3841 attaatccat caataaagga ttggatcatt ttttctctcc tccttttact aaatgtctga
     3901 tgatctcacc ataggccaat tgaaggttct tcgatgtaaa gacttcttgt actggaccgg
     3961 aagcgatcag ctgtttattt aaaagaataa cctcatcgaa atattcttct actttatgaa
     4021 gatcatgatg gacgatcaaa atcgtttttc ctgaattctt caattcctgc aagatgtcaa
     4081 agatctttcg ttcacttaac gcatcaatcc ctacgaatgg ttcatctaaa aagatccatt
     4141 ctgctccttg ggctagagct cttgcaataa aaactctctg aagctgtcca cccgatagtt
     4201 cgctgatctg tcttttggca tattcttcca tccctacttt tttcaaagct tgtcttgcac
     4261 gttctttttc ttttttccca ggtctttgtc caattcgtaa agatggatat gttcctaaaa
     4321 gtacaacgcc taatacatca attggaaagg aaagatccaa ttcacttcgc tgttccacgt
     4381 aggctatttt tttacggact gagtttaccg gctgtccaga aaaggtgact ttaccagtag
     4441 tagctggtat caactctagc aaagatttca taaatgttga ttttcctgca ccgtttggtc
     4501 cgataatacc ggtaattttt tgtggattta tcgtcaagtt gatagttttc aaaacagttt
     4561 tcccttgata agcggctgaa aggtcttgaa ttacaatggc cgtacgttcc aaatcttaca
     4621 ctccttttta ggtttaccta actttttatt taaatatagt ttaactaaaa tggaaagcat
     4681 tttcaagtag aatgatgaaa aagttttttt cgaattttcc tcgcatttta tgaaaatttt
     4741 ctgtagaatc acatcaagag ttcaataagg ctaacctaat aatgaaaaac atgtgttgaa
     4801 acgtttgatt attttttctt gttgactcaa caacgaaagg agtttttgta tggaagaaga
     4861 aaagttaact cgaagtctta gtgcccgtca tatccaaatg atcgcactag gtggaacgat
     4921 cggtgtcggt ctgtttatgg gtgcctcctc aaccatacgt tggacagggc cctcagtgat
     4981 gggagcctat gcgattgcag gattcttttt gtatttgatt atgcgtgctt taggcgaaat
     5041 gctttacgtt gatccttcga ctggttcttt tgcaaattac gcgagcgagt acatccatcc
     5101 tgtggctggc tacttgaccg cttggagtaa tatttttcaa tatattgtcg taggaataag
     5161 tgaagtgatt gctgtaggat cttatatgaa ttattggtgg cctgatttgc cggcaattat
     5221 tcccggaatc attgtcgtct tgtttcttat gctggcaaat cttatttctg tcaaggcgtt
     5281 tggcgaatta gaattttggt tttcgatcat caaagtcata acgatcattt tgatgatcat
     5341 tgcaggtctt ggggtaatct tatttggatt tggtaatcat ggtcaggcag tagggatttc
     5401 aaatctttgg aaaaatggtg gattcttcac tggaggcgtc aaaggatttt tctttgcatt
     5461 atcgatcgtt gttgcttcct atcaaggaat cgaactgatc ggaatgactg ctggtgaagc
     5521 agagaatcct aaaaagacga tcattgaagc tgtccagtcc actatcggtc gaatcttgat
     5581 tttttatatt ggtgctattt ttatcattgt gagtatctat ccgtggaatc aattgagtga
     5641 gattggttca ccttttgtac aaactttttc taaagtcggc attacatttg ctgcaggact
     5701 gattaatttt gtcgtgatta cagcggcatt atcaggttgc aattcaggga ttttcagcgc
     5761 aagtcgtatg gtctatacac ttgccataaa tggaaagatg tctaaaaaat ttctgaaact
     5821 atctcgtcac ggtgcgcctt tttatcctgt cgtagcaatc tcaggtggaa tcttaattgg
     5881 tctgatctta aattatcttc taccaatgtt catcaaccag tcagaaaata ttttcgtttt
     5941 cgtatacagc tcaagtattt tgcctggtat ggttccttgg gtagtgattt tgatcagcga
     6001 gattcgcttc aaaaagaagc acgcagataa aatgcatgat catcctttta aaatgccact
     6061 atctccttat acgaattatg tgacacttgc ttttttagct atcgtattga ttttcatgtt
     6121 tatcaatcct gaaacgagaa tctcattgat tattggactc gttttcttgc tttacatgac
     6181 gatccattac tttgtacgcg aaaaaagaac cggaaaataa aaaaagaacg aagcttcacc
     6241 ttagtggaag cttcgttctt ttttattgct tttatcaatt caattttagc gtctcaccgg
     6301 gagtcaggaa ataattctcc atgctcatcc cattcagcga caagatcgtt tctactggta
     6361 cgccagttcg ttcggctatt tgatttggac cttctccttc caaaactacg gttgtcgcag
     6421 cctgtgaagt cgcgctgttg gtattagttt cagaggaatc tgttgtgtct tgtgttgttc
     6481 gagcttgcat ttcagaagct tctaccgact ctgctgtctc agaatcagaa gatgttgtgt
     6541 cttgtgtgct tgttgactct gcttgaagag aggcgctagt ctgcaaataa atatcggaaa
     6601 tcttagcgac atgattcgta taattagccg ataattgatt gatcaaagta tctaagtcta
     6661 caccgtcttt attttctgct gcctgatacg ctgcacgtaa ttttctgatc ccttcgtcgg
     6721 agattgcttg aagctcacgt gctttttggt tagcaactgc tgataaacca gctattccct
     6781 cttgatttcc ctgaatttct gtctggtatt cttcaatcaa tgcaggcgtc ttttcttcga
     6841 tctttttcga ataatcattt acgattgctt gaagttgctc tggcaaacca tttgctgttt
     6901 tttcttcaga agtttgtgtc tcttttgttt tagttgatga ctcacttgtt tgtttcgtct
     6961 ctgttttctt tgtcgttgtt tccgttatgg tcgtactttt cgcttctgac gaattttttc
     7021 catgaaaata tagacccgca actcctcctg ctatgatcaa aaacacggca actgctgccc
     7081 caatcatgat catccgctta cgagctgcct gtttttgctg tcttcttcgg gtcatccgaa
     7141 cattttctcc ccactcattt tcatcataca tttttttcac caccaatttt tatgtataat
     7201 agcgttatca aacgcttcat attcttattg tagcaaataa ttgtcgctgt gcacattttt
     7261 atctttgtta tttataagaa aatttcacca gttgagatga ttccaagatt gtccgtcgat
     7321 ttctccttgc agctgcattg ttcgttcgtc tatctcagcc atcagtttct ttatttcata
     7381 taagagcgca cggtcttgat attctgattt ttcgcttaat tcatctaatt gttggatcag
     7441 ccaatcattt gtcataatga agttcctttc atttcttgca gtgtaaaggt caagtcttcg
     7501 atctgctctt ttgcctcagt cagtgcttct acgatatatg cttgtttttc agattcttct
     7561 tcctgcattt tctgattttc ctgtactctt tgaataaacc agtcaatccg ctgagtcagc
     7621 gatggtttgc cttccaatga gagatactgc tgataagcaa ttagcttctc ccaacgtttt
     7681 tcctgggata aatacataaa ttgtgctaaa acgaatggcg gaagataggt catatggaat
     7741 ttatttgcga ttcgctgata cggcctcaaa aattcggaaa tcgtatattc ttcttttcct
     7801 ccagcttggt attctttttc cgccacacct gttgtcaata cgattcctag ttctttccct
     7861 tttaatacat gtgctgctcg ttccaagaca ttatcttgcc aaatttttaa atgagcagga
     7921 gaactatacc agtaaaacgg aaactggaaa atgatccgat catgctgggc caagagctgt
     7981 agttcagtct ctatatctat ttttccgtct ggataacttg attccaaatg atgccaagta
     8041 gctggtaaac cagcagaagc ttctttgaaa aattgctgat ttaaagattc ttcgatcgtt
     8101 ggatggctaa tgatgataag tgtacgcatc ccttttcctc ctttttagtc ttcgctttac
     8161 agtgtagcat aaccgccaca agtttctttt gccgatgctt gtcattttaa ccagactaat
     8221 caggttgtcc aagaaactgc ggtttgtttt tccaactgtt ttttcaaggt atccaaagca
     8281 atattcttaa aattttcgtt tttcatacgt ttacgatagt tcgtagccgt ttctccggta
     8341 ctctttttga acattcggct aaatacgact agactattga accctacttg ataggcaata
     8401 ctttccacgg attcatccgt cagagaaaga agaaacgccc ctcgattgat ccgtaccccg
     8461 atcaaatatt ccttgatact ggtacccata tgatttttga atactcgcga caaatggctc
     8521 cgattgaccg ctaaatgctg ggcgatttcc tcaacagaca atttttcagc ataatgtgcc
     8581 tcgatgtatt tgactgtttc aacagctaaa gaggaatgaa ccttttgtac accaccgctg
     8641 acctttccgc catcttttaa aaggactgat aaaaaacggt aggttaatgc tgttaactgt
     8701 aattcatttt ccaaggtttc ttccgtatct ttcatgcatt ctaaaataat gtcgatatat
     8761 ttttgtatgt cagaagagac catcgtgtat tggtcttctc tgaaaagcga ctgccgaatg
     8821 atttcttctg cagcactccc accaaatgaa agccagcaat acatccacgg atcttctcca
     8881 tctgataagt aaaacgtcga gtctcctgga cgaatcaaga acagatcacc ttttttcaat
     8941 tggaattgtt gatttttcac atgataagat cctttgcctt ccatcacgac atgcaggatg
     9001 taatcattgc gaatagcagg accaaaagaa tgaaacggca tcgttttaga gaaaccacaa
     9061 aaatcaaaat acaattcttt ttctacttgt tgatgttcct tttttaaaaa aatcgattgc
     9121 tccacgtcta tccctcctta ctcctttagt ataatgcaac aaaaagttaa ataaaagtca
     9181 cagaaatata ttaaaaagga agcgatttct tgttaagttt aagtcattaa aagatctttg
     9241 ttattcgttg taaccggttt acttagtatt atatattgaa caggagagga atacagatta
     9301 tgaaaagcaa aatgatccaa cgtgtgtcct acgctttagg cgcttttgga cacgacacat
     9361 tctatgttac attaagcact tattttatgg tattcgtgac aagccagatg ttcgcaggaa
     9421 cagataaggc aacgaatgca aaaatgattg ctacagttac aagcttagtt gtaggtatcc
     9481 gcttagttga aattattttt gatcctttga ttggtggcat catcgataat actcgtacaa
     9541 aatacggtaa attcaaacca tggttagtta tcggcggcgt tgttagttcc gtactcttgg
     9601 tagcaatctt taccaatttc tttggcttag ctactcataa tacgacttta tttatcatat
     9661 tatttgcaat cgttttcatc ttattggata gtttttattc atttaaggat atcgcttttt
     9721 ggtcaatgat tccagctttg agtacggaaa caaaggaacg tgaaaaattt gctacgtttg
     9781 ctcgtttcgg ctcttctcta ggtgcaaatg gtactactct agtcgttgtc cctatcgtta
     9841 ctttctttac ctatcttgtt actcataaac atattgaagg cgcttcaggc tggttctggt
     9901 ttggtgtgat tgctgctgtt atttcaggcg gtactgcgat tatcactgcc ttgggcacaa
     9961 aagaagtcga tactgcgatc cgtcagcaaa atgaaaaagc tgggatcaaa gatgtgttca
    10021 aagcagttgc tcaaaatgat cagttgatgt ggcttgccct tgcttatatc gcttatgcga
    10081 ttgccaatgt agcaacaact gccgtattat tctacttctt caaatatgta cttggccaac
    10141 caactgaatt ttggatcgtt ggtctagttg ctgccttact tggtttagta gctgttccac
    10201 tattcccaat gcttacacgc gtcatctctc gtcgctacgt ttaccttgga gggatcgcaa
    10261 tgatgatttc ttcttacttt ttctttacga tcgcaggttc aagtttgcca atcgttatca
    10321 tcgggctagt attgttctac ttccctcaac aattgatttt cttatccgct ttgatgacga
    10381 tcacagactc tgttgaatat ggtcaatgga aaaatggtat ccgtaacgaa gctgttactc
    10441 tatcaattcg ccctttacta gataaaatcg ctggtgcttt gtctaacggg atcgtagggt
    10501 ttgtcgctat cgcttctggg atgactggta atgctactgc tgctgatatc actgctcatg
    10561 gcgtaacgac tttcaaagct tatgcattct atgctcctgc tgctttgatg attttctccg
    10621 ctattatttt tgcttggaag attacattga cagagaaaaa acatgcacaa atcgttgagg
    10681 aattggaaaa caaattggct ccagctgata caatggaaga tgaacttcac gatgcagtag
    10741 ttccttccca ttaagaaagg atcttgaaaa tgaagattac agaaaaagat tttggccaag
    10801 gttatcactt gataaccttg gagaacaaaa acaagctagc actttctatc tctgatttag
    10861 gtgctagaat cgtcagttta aaatcaaatg atcgagaatt agttttggga tttgacaccg
    10921 ctgaagaata tatcgaaaaa gatccgtata tcggtgcttc tatcggtcgc accgcaggga
    10981 gaatcgaaaa tggccgcttt tctctcaatg gaaaaactta ccaattagcc acagatccaa
    11041 aaactggaca taatctgcat ggtggtgctc ctggatttga attaaaaaaa tggtcttatg
    11101 tcattttgaa tggcgaaaat gaagcttctg tcatttttac tactacaagt cccgacggag
    11161 aacatggttt tccaggtaca atggatgtgg aaatccgcta tactttgaca aaagataata
    11221 tctggcgagt gacctctcgc ggcaccagtg accaagatac tttattcaat ccgacaaatc
    11281 atgtttactt caacttgacg ggagatgctt ctcagtcaat cgatcaacat gagctttggc
    11341 tcaatagcga agcttatgct cctttgcgaa cagatagtat tcctataggg gtaaaagaaa
    11401 atgctgcagg aagtgctttc gattttcaaa tacctaaaaa attagcgtcc gtttttgcta
    11461 gtgatttgga tcaaaaaaat cttgttgacg gaatcgatca tccatttttc ttaaaagaaa
    11521 ccggcttggg taaagaagcc gctcgcttga ctagccccga taaaagaatc caagtaggta
    11581 ttgctacaga tgcttctagt gtggttattt tcactgcaaa tttcggcaca gaaacaccag
    11641 aaatgcgcaa ccgaaaactg gcacatcacg gtggcatcac atttgagact caaacagctc
    11701 caggtgcaga gcgcttctca gcatttggtt cgattcattt aaaagccgga tcagtatttg
    11761 aaacagtgac agaattcaaa atcaaaacaa gaaaggaata actcatcatg gaaatcaaaa
    11821 gaagcttaaa cacaaaattt gccgaaacat tcggacctaa aaaaactaca caatacttct
    11881 caccaggacg catcaatttg attggtgaac acacagatta caatggaggc catgtcttcc
    11941 ccgcttccat cacttatgga acgcaaggtg tagcagcacc tcgagacgat aataaaatat
    12001 tagtttattc cacaaacttt gacgatatcg gtgtcattag ttttacttta gatgagctag
    12061 aatatgataa aaaagcaaac tgggcaaatt acgtaaaagg aatggtcctt aaattaaaag
    12121 aagctggaca ctcaatcgat catgggtttg agttgttagt ggaaggaaca atccctaatg
    12181 gtgcaggact ttccagtagc gcttcattag aattattagt aggtgttgta ttagaagatc
    12241 tgtttgattt aaaaacagat cgcttggaac tagtccaaac aggtaaaaaa gtagaaaatg
    12301 aattcatcgg tgtcaattca ggtatcatgg atcagtttgc catcggcttc ggtgagctag
    12361 atcatgcaat cttattagat acaaatacgc tgaaatacga aatggttcca gtgaaactag
    12421 atggttatgc aatcgtcatc atgaatacga ataagcggcg ggaattggca gattcaaaat
    12481 acaatgaacg ccgtagcgaa tgcgaagaag ccttggcacg tttgcaaaaa actttgccta
    12541 tctcttcttt aggagactta gatgaagaag aatttgaatc aactattgac caaatcggcg
    12601 atgatacatt gatacgcaga gctcgccatg ccgtttctga aaatcagcgg actttgaagg
    12661 caaaagctga attagaagct gggaacttag aagcatttgg tcaactgtta aatgattctc
    12721 accactctct tcgctatgat tatgaagtaa ctgggatcga attagataca ctagtagatg
    12781 ctgctcaaaa acaagaaggt gtcttaggtg cacgaatgac aggagctgga tttggtggct
    12841 gtgcgattgc tcttgtcaaa gaagaaaata tacctgcctt caaaaacaat gtctatgatg
    12901 aatacatgaa agttgtcggc tatgctccgg aattttatgt tgcacacatt ggaaatggga
    12961 caacaaagat cgatccagaa gctgttcatg tttgataaaa aaatcaacga tctcagctaa
    13021 aaacaaattg ataaaaataa cctctcagca atttccaaat gtgagccttt tgctcgtatt
    13081 tgaaaattgc tgttttttat tgacacggaa agagaacgtg tgttctaatt gtgttgaaga
    13141 ggtgattgtt atgtgggcga atcaaaatcc ggtatttgtt tatgaaaaag aaccttcaag
    13201 agatatttta tgtattgact gcaaatcttt ttacgcctcc gtagagtgcg ttgaaaagga
    13261 tcttgatccc cttacaacta aattagtagt catgtcctat ccgtccgatt cgacagaaac
    13321 acggggtagc ggattaatct tggcttccag tcctgcagca aaaaaagctt acggcatcac
    13381 caatatcagc cgtgccagag atcttccctt cccctatcca agtgatttat atattgttgc
    13441 cccaaggatg gcctattata tggaaaagaa cagagaaatc aattctatct ataaaaaata
    13501 tgcagacgaa gcgaaccatc atgtctattc cgtcgatgaa agcttccttg atgtaacaaa
    13561 tagcatggca ctctttggta aaacaaatgc gtacgacatg gctaaactga tccataaaga
    13621 tgtgtttgag cagaccggaa tctacacgac tataggaatt ggcgataacc cgctacttgc
    13681 taaactagcc ttagacaatg gctcaaaaga cggaaagcaa atgtttgctg aatggcgtta
    13741 tgaagatgta ccagaaaagt tatggaaaat ctcagatatc actgactttt ggggaatcgg
    13801 caaacgaaca gcaaaacgtc tgaaacaaat gggtatacat tctgtttatg atttagctca
    13861 cgcggattat tatcacttga aaagcaagct aggggtcatc ggtactcagc tttatgccca
    13921 tgcttggggc atcgatcgaa gttttttggg acaagaatat acaccaaaat caaaaagtat
    13981 cggaaatagt caaatcctgg atagagatta tacaagacga gaagaaatcg agatcgttat
    14041 caaagaaatg gccgaccaag tcggcactcg cttgagacga tctgcagcaa aaacacaagt
    14101 agtcagctta tgggtaggat actctttagg ctatcttgat ccaaatggca agaccgggtt
    14161 tcaccagcaa atgaaaatcg aagcgacaaa tgctagcaga gatctagcag atgctttatt
    14221 gaccattttc caccagcatt accactacca ggatattcgg cgtgtaggcg tgaactgttc
    14281 cgacctcgta tacgcgacag gtttacagct taaccttttt gaagatcctg aaaagcagat
    14341 aaatgaagcg aaagttgatt ttgttgtcga tacgattaga aaaaaatacg gctttcaatc
    14401 aatcatccat gcgcatagtc tcttgcaagg aggacgcgcc atcgcacgta gttctctcgt
    14461 tggcgggcat gcaggtggta tgagtggatt agaaggtgga aaagatgaga cggacagcaa
    14521 aaaaattcat tgactataac gagtatcgag atcggccatt tggattaaaa tgggggactg
    14581 cttttgcgat ggaagaacta gtcaacggta tcaaaaaaaa tgaagaagaa gcacgcaaac
    14641 agcctcttcc taaacaacaa atgacattgc aagaaatcga tagtatactg acagaagcct
    14701 ttttatctcg taaagcagta atcattcagc tcaatttaaa agatgaattt ggaagactct
    14761 tggatgaggt cgaagggcac ttcattggtg aagcctatga agattacttt atgattgacc
    14821 atcagccgat attgtgggaa gacgtacggt atattgagtt aaaggaaaca aaaaaatggt
    14881 ttgtgacatg agaggttgtg acagaagtga tcaacttcaa aaaataagaa aggatttttc
    14941 gaaaattgct tcttaaattt ttgtggaatt ttggcttatt tttcgaagaa gcaacttctg
    15001 gaacaacgta tattagatat cagaggttgt gaaaaagtcg cttagttttg agtaacaagc
    15061 caaggcgctg atttcacaac ctctgtctat ctatccatct attcggtaag attcatgcat
    15121 gacgtcttct ttatttagta tgtagagccc tttttcttct gaccattcaa cataaatcag
    15181 ctgttttaca tctgcatagc cttctttctt caagttctcc ataagccatg cttcagtcaa
    15241 ttgattggac tgaagcactt tatgctgaat ttgcccttca tctacaagca ggatcgataa
    15301 agctttgtct tcatctgatt ttttcaatac actcatttga ccggcattct ctaggataac
    15361 atgtttggca ttttcaagag aaaagcattc ttgctgtctt aagattgctc gcaactgctc
    15421 catctcgata tggttatttg tcaactctgt catgttgatg acgccgtctt tgattagaac
    15481 agatggctcg cctttgaccc aacgattgac cttttcattt ttttggacga tacgttcgat
    15541 accataaatc attactgccc aaagcgcaat ggcaaatagg acatgcccta catggacatt
    15601 gtcatcatag gttgattctt ctaagatccc acctaaaaca agcgtataga ctaaatcaaa
    15661 cggtgtgatt tctgacatgg atttcttccc aagcaaacgt accactaata ataaaccgat
    15721 caacccgaac accagtttca ttgtaattga aaaaatcatt ttttctcccc ctaactatct
    15781 gttaattaaa ttatatatgc tttctcacga acacgcatgg aatatacaag gaagaaatgt
    15841 atccttttac taaaaaaagg actgtgacac tgtagtgacc cccaaaagtt agactttttt
    15901 ggagtggaga aatccactcc ttttttttat gctctaaaat gatattttta cacaaccgtt
    15961 acggttgttt tacg
//
LOCUS       DAYYNG010000059        15572 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000059 DAYYNG010000000
VERSION     DAYYNG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.059"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..462
                     /locus_tag="ACHBFA_001487"
     CDS             <1..462
                     /locus_tag="ACHBFA_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9394982.1"
                     /translation="NGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKN
                     NSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDK
                     GISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            662..976
                     /locus_tag="ACHBFA_001488"
     CDS             662..976
                     /locus_tag="ACHBFA_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9394983.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            997..1374
                     /locus_tag="ACHBFA_001489"
     CDS             997..1374
                     /locus_tag="ACHBFA_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9394984.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1384..2157
                     /locus_tag="ACHBFA_001490"
     CDS             1384..2157
                     /locus_tag="ACHBFA_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394985.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2179..3582
                     /locus_tag="ACHBFA_001491"
     CDS             2179..3582
                     /locus_tag="ACHBFA_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9394986.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3764..4948
                     /gene="mobT"
                     /locus_tag="ACHBFA_001492"
     CDS             3764..4948
                     /gene="mobT"
                     /locus_tag="ACHBFA_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9394987.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4945..5235
                     /locus_tag="ACHBFA_001493"
     CDS             4945..5235
                     /locus_tag="ACHBFA_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394988.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5232..5453
                     /locus_tag="ACHBFA_001494"
     CDS             5232..5453
                     /locus_tag="ACHBFA_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9394989.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5495..6274
                     /locus_tag="ACHBFA_001495"
     CDS             5495..6274
                     /locus_tag="ACHBFA_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM9394990.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6335..6835
                     /locus_tag="ACHBFA_001496"
     CDS             6335..6835
                     /locus_tag="ACHBFA_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9394991.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6891..7529
                     /locus_tag="ACHBFA_001497"
     CDS             6891..7529
                     /locus_tag="ACHBFA_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM9394992.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7601..7996
                     /locus_tag="ACHBFA_001498"
     CDS             7601..7996
                     /locus_tag="ACHBFA_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9394993.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7980..10433
                     /locus_tag="ACHBFA_001499"
     CDS             7980..10433
                     /locus_tag="ACHBFA_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9394994.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10430..12457
                     /locus_tag="ACHBFA_001500"
     CDS             10430..12457
                     /locus_tag="ACHBFA_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9394995.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12454..13476
                     /locus_tag="ACHBFA_001501"
     CDS             12454..13476
                     /locus_tag="ACHBFA_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM9394996.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13493..14422
                     /locus_tag="ACHBFA_001502"
     CDS             13493..14422
                     /locus_tag="ACHBFA_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9394997.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14736..14783
                     /locus_tag="ACHBFA_001503"
                     /pseudo
     CDS             14736..14783
                     /locus_tag="ACHBFA_001503"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14799..>15572
                     /locus_tag="ACHBFA_001504"
     CDS             14799..>15572
                     /locus_tag="ACHBFA_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9394998.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEY"
BASE COUNT         5295 a         2590 c         3083 g         4604 t
ORIGIN      
        1 aacggtgttg atttatacaa atttgttacc aaacatatat ttgttaatct taatattgac
       61 gagaaaaatt acccgtctat ttttaagcac tatctatttt ttattaacga agtagaagat
      121 cggataaaaa ataattcatt atctagtagt gtatctagtt ttaagaagat gtttaaatct
      181 cgagatggta tcacacttac tacagcacat aaagttaaag gaaatgagta tgatactgtt
      241 atcgctatta atcttctaaa cgggaaaatt cctcattgga atgaaatttt taatgtttct
      301 gataaaggaa ttagttcagc gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat
      361 aatttatttc ttttctcaga ggtgggttat tttacgagta aaggagactt gctttatccc
      421 actcaattat tagataaaat agaatttgat tataatattt aaaataccta ggtagtcgtt
      481 gttttaaata tagtaggaat cggacgctca gtacggagcg tctatttttt tacccaattt
      541 tgaaaggaag tgaacttatg aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct
      601 ttaaaaccat cacacaaata agattcattc aaacataaaa acagaaagga taggtaaaaa
      661 tatggaactt aaatatgtca ttcccaatat ggaaaaaaca tttggacaat tagaatatgc
      721 tggtgaaggg aacattgaac aacgtcgaat aaatggcaga ccaaccattc tctctcgtag
      781 ttacaattta tactcaacga ttcaacgagc agatgatatt gttgtcattc ttccctccga
      841 agctggcgag aaacattttg aatccgacga aaaagtcaaa ctcatcaacc cacatattac
      901 cgcagaaggt tataaaattg gcaatcgagg atttaccaat tacattatgc acgcagacga
      961 tatggtgaaa gcataagaaa ggaaaggtga aaaaatatga gattagcaga aggtattgtg
     1021 attgaaaaag accagacatt tggcacattg aaattttccg ccttgcgtcg tgaggttcgt
     1081 atccagaatg aagatggtac ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa
     1141 tcaaaaggac aaggacgcat gatacaagtc agtatccccg cttctgttcc tttgaaagaa
     1201 tttgactaca acgcagaagt ggaattaatc aatcctgtgg cagataccgt agcgacggct
     1261 acttttcgag gggcagacgt tgattggtac gtgaaagcag acgatcttgt attaaaagga
     1321 cagacaaaac ctcatcagaa atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg
     1381 cttatgacaa acacaccatt gattttgaaa gaaattccga aggatgaagc catttctttt
     1441 attcggcaat accattattc aaagatactc cctagattgt gtaaatactt tctagggatt
     1501 ttttcagaag aaaaattatt aggcgttgtg gaattaggct ggggtacgca acccctacaa
     1561 accatacgca aactctttcc cgacagttcc cttcaaacca cagattattt agaaattgga
     1621 aagatgtgtt ttcttccaga aatgaatcaa accaattatt ttggcagtca agctctttca
     1681 gctctaatta agtggttgaa agaacatacg gactgtcatt ttttatatac actggcagat
     1741 ggaattgagg gaaaatgtgg ctatgtctat caagcgtcca acttctttta ctgcgggtat
     1801 ttcaagacaa gtgtctatcg tgataagcag tcatgggaaa agatacaccc tcgaagtgct
     1861 cgccttttat tagaggaaaa tgctcgcttt gaacaggtag agaaaaagca ttggctttcc
     1921 caggcatttt gcgaatacaa agggattgag aaaatcaatg gacgcatgtt tcgctatctc
     1981 tatccgttga caaaagaagc gaaaaaactg ttaggacaca ctctctatcg acgtcattat
     2041 tatccgaaag aaaaaaattt acgctttgaa aagcgaatcg cttatcagaa atatgaagct
     2101 atttctcaac caacttttga taaacaggct cgtatctaca atacacaatt attttgatag
     2161 gtggaaagga ggatttttat gccattgctt catgacagag gagagcgaat caaagccaac
     2221 gataagcact tggtctatca ttttgtacta atttcattgc tggtagtttt tctagtcgtc
     2281 ctactccctt ttcattggag agcgttacaa tcggtgaact ggcaagcagt tcagttagat
     2341 acatttttct atcaacttca tacgccatac ttttggagca gtattctcac tgctcttttt
     2401 gtatgcggac ttatcagctg gctttattat cactatcgta tagattctat aaaacagtta
     2461 gagcaccgtc aaaagttagc tcgtatgatt ttagaaaatc attggtatga agccgaagaa
     2521 actcaatcag agggattctt taaagattta cccagcaaga aaaccaaaca acgaatccgc
     2581 cattttccta aaatatatta tcgaatgaaa gatggactaa tccatattca tgtagaaatc
     2641 accttaggga aatatcaaga tcagcttttg cacctagaaa acaaactaga aacaggattg
     2701 tattgtgaat tagtggaaaa aacgttgaaa gattcttatg tagagtatgt cctactttat
     2761 gatactattg gcaatcgaat tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa
     2821 ttgatggaat cggtctattg ggagttcgat tctctccctc acatgttaat tgctgggggc
     2881 actggtagtg gaaaatccta ttttattctc acattgattg aatctctcct gcatacaaat
     2941 gcaaagcttt atattcttga ccctaaaaat gctgatttgg cagacttagg ggcggtcatg
     3001 aataatgtct attaccgtaa agaagatatg ttggcatgta tcaatcaatt ttacgaagat
     3061 atgattgatc gcagtgaaac catgaaacaa caccctaact ataaaacagg agaaaattat
     3121 gcttatcttg gtttgtctgc caattttctt atttttgatg aatatgtggc aatgatggat
     3181 atgctaggtc gtgaaagtac caccgttatt cataaactca aacaaattgt aatgttagga
     3241 cgacaagcag gcttcttttt aattcttgcc tgtcaacgtc cagacgcaaa atattttagt
     3301 gatgggattc gggacaactt taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc
     3361 tttggtatga tgttcggaag cgatacacaa aaacaatttt tcttaaaacc catcaaggga
     3421 agaggttacg tggatacagg aaaaagtgtg attagtgagt tttatacacc ccttgtacca
     3481 aaaaggtacg atttcttagg agaaatagga aaagtcattc aaaaaaagca atctgagcct
     3541 gtaccgcacg aagtgaaagg tacaggttca gacttgcttt gattggtgtg gcgttagcca
     3601 cgccaatcct ttagccccct ctatctaaca ggggggcaca aatacaactg aaaacaggca
     3661 attctcatca accaatgttg atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact
     3721 caagggtcat agtttacatc ttttttagat tggaggtttg cgaatggaaa acgaaaaaat
     3781 ttggattaag gaattgaaag aaaagcgact ggcttatggc gtttctcaaa ataaactggc
     3841 ggttgcttct catattacca gaccttacct atcggatatt gaaacaggca aggcagtccc
     3901 tactcaacag gtgaaagaag atttactgaa cgctttggaa cgcttcaatc ccgataaccc
     3961 attggagatg ttattcgact atgtacggat tcgattccct accaatgatg tggctcaaat
     4021 tatcggtgag gttttacgtt tgaatatgga ttatatgcta cacgaagact ttggctatta
     4081 ttcttatccc gaacactatc gtttcggtga tattgttgtc ctagtctctc atgatgtaag
     4141 taaaggtgtt ttattagaac tgaaaggaaa aggctgtcgg caatttgaaa actttctatt
     4201 ggctcaacat cggagctggt atgatttctt tcaagattgc atggaacata aaggtatttt
     4261 caaacgcttg gatttagcta tcaacgataa aacagggatt ttgaatattc cagatttggc
     4321 aaagaaatgt aaacaggaag aatgcatttc cgtctttcga agctttaaaa attatcgcag
     4381 tggtgaattg gttcaccgtg atgaaaaacc atacatggga aacacacttt atattggctc
     4441 attaaaaagt gaagtctatt tctgtatata tgagaaagat tatgagcaat acgtgaaaaa
     4501 tgatgttccc ttagaagacg cagaagtaaa gaatcggttt gaaattcggt taaaaaatga
     4561 ccgagccacc caagccatga aagatttatt ggcacatcaa caggcggaaa aaacagcctt
     4621 tgaaattatc aatcgctata tccgctttgc agataaagac gacactaaac gtcggagtga
     4681 ttggaaaact aatgaacgtt gggaatggtt tatcggtaaa aatcgtggag cattacgcct
     4741 aaccacacag ccagagcctt attcttttga acgaacattg aactggttac atcatcaagt
     4801 cgcccctaca ttaaaaatcg caagcatttt agatgttctc aacggtacaa ccattatctc
     4861 caccatgatt caggaagcca aactgacaga aaaacatgag aaattaattg aacaacaaca
     4921 tctagccatg gaggatttga ttacatgagc cttttaacaa aagaattaaa gaagttaggg
     4981 tttcagtgtg gcattgagtt tcaagcgtat atacaaaata cagggaaata tacgtctctc
     5041 attattgagg gaaaacggca agctggtgat accatctata cctatgattt ttacaaggtg
     5101 agattttata ataattatac caatcgagta accgtctatg gagagcattt aacccctttt
     5161 caattattaa ggcgtgtaaa aagctatatc tattatcgag aaaaatatct taaggaaagg
     5221 aggacaatca catgaatttt gggcagaatt tgtacaactg gttcttggat aatgcacagt
     5281 cattggtatt gttagcgatt gttgtcattg gactgttttt aggttttaaa cgagaatttt
     5341 caaaactcat tggcttcttg attattgctc taatcgccgt cggacttgtg ttcaatgcag
     5401 ctggcgtgaa agatgtgtta ttaaacttat ttaaccgaat cattggtgct taacagatat
     5461 ttttcatgat aaaataggaa gtatatattt tgttatgaaa ggatttatga atatgtttac
     5521 aaatgatcaa tttaaatctc ttttaattaa aacattagaa agaaagagct cttcaaatta
     5581 ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa ctttagatga
     5641 gactgacgat tttacttatt ataaaggata taaaaagggc tggaatacgc tttgcacata
     5701 tcttaaaata agagttcctt ttgatgattt agactttttt gaaagtcata aagatttgat
     5761 tacccagaca gcaagctcca tatatgataa acaaggtgat aatgtattag tggatactat
     5821 tttagtacca cttccagaga attacgaagt aattaacttt agtcagttga aaattagtga
     5881 tgtggtttca caagctattg aagacgctga gtcttttatg agtaatggtg aatatcaacg
     5941 tgcctttgac agagttcaca ctgcttttca tggatattta attgaaatac taaaaaaata
     6001 tgaaattaca gttcctagag acgaaaatct atctaaatta tatagtagga tacaacaatt
     6061 aattgaaaaa gaaattcaac ctactgaact tgctgatata gtgaagacaa cgataaggtc
     6121 atcaaatgga atgattagct ctcttaatga agcaagaaat aggcattcac ttgcacaccc
     6181 taatacaaat attattggaa agagagaagc aaaactaata ataggtattt caagtacagt
     6241 tacagattat attagcggat accttgataa gtagcgtcat taactgattg ctactttttt
     6301 tatgactatt tttataaaaa ggatgtgata agaattggaa atgaaagtct atgtggcaaa
     6361 tttaggacgg tataatgaag gtgaattagt cggagcatgg tttactccac caattgatga
     6421 ggaagaaatg gcagaaagaa tcggtttaaa tgaggactac gaagaatatg caatacatga
     6481 ttttgagctt ccttttgatg tggacgaata cacgccgatt agtgaaatca accgtctatg
     6541 tgaagccatt caagaaatcg aaggtacacc tatttataat gaactcaaag aaattcaagg
     6601 catgtggttt agtagcttgg aagaattact ggaaaacaaa gaggatattc attgttatag
     6661 tgattgtgat tcgatggaag atgtcgcccg ttattacgtg gaagaaacag gacagcttgg
     6721 tgaagtccct tccaatttac aaaattatat tgactaccaa tctttgggac gtgatatgga
     6781 aatagagggt aattacctag ttacctctca tggggtattt gaatattgtc agtaagttta
     6841 acaatccgtg ttataattaa attaataaat agcacggaga ggtataaatt atggtaaatg
     6901 tatttgaaaa tcttaaacta ttaaaactag acatgaaaga taaaggctgg gtgatagatt
     6961 ccttttactt tcgttacaaa cagcaaaatt atattgtact tgttaagcta tttgaaaaag
     7021 aggaaaaagt tccagaatat gctctgttaa aactagaatt tttaaaggaa aacgattttt
     7081 cagatatgct agctgtctat gctaattcgg taaaattata tacagacacg aaaacaataa
     7141 gagaatattt tggtatcgaa tacagtagta acttgggaga tgttttattt caatttagcc
     7201 aaactcttgc tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag
     7261 aagccatgtg tttctcatta agtcaaagtg attcagaaga tcccagaaaa aaatattgtt
     7321 tttcagttag aagaaatcct ttaaagggaa atggtgagct gggaaaacga agtcctttta
     7381 atgacaataa aacgagattg tatagaccca gcttatatga acgattaggg tcagatacca
     7441 atttaagttt cagatattca atgaatccag atgatgaaga aactgatgaa ggtattattg
     7501 caaaatggac aaaaaataaa attgaatagt taaagcattg aaagagcaat cacctatttt
     7561 gattgttctt tttctatttt tacggaaagg acgtgaagac atgaaaaaaa taagaagtta
     7621 caccagtatt tggtcagtag aaaaagtgat ttatgcgatt aatgatttac aactcccttt
     7681 cccaattacc tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg tcatactctt
     7741 tggcagttta ccaccacttt ctctgattga cggagcacta ctcaaatact tagggattcc
     7801 cattgttctc actgggttca tgtgccaaaa gacctttgac ggaaagaaac cctatggatt
     7861 tctaaagtct atcattacct atgctctacg ccctaaagta acctacgctg gcaaacaaat
     7921 acgattaaaa aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg aacaactaga
     7981 tggcatatcc gattaaatat atggaagata atctcgtatt taatcatgat ggcgaagtat
     8041 tcgcttatta tgaattaatt ccctataatt acagtttttt aagttccgat gaaaaaattc
     8101 aagttcatga taatttctgt caattgattg cccaaaatcg tgatggcaag attcatgctc
     8161 tgcaactagc aaccgaatca agtattcgag ccacacagga acaatcgaaa aaagaaatca
     8221 ctggacgatt aaaagagata gcttacgaaa aaatagatga acagaccgaa gcgttaattt
     8281 ctatgattgg tgacaatcaa gtagactatc gcttttttat tggttttaaa ctctacctca
     8341 atgaacagga agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat
     8401 tctttcatgg tgtgaatcac aaattaatgg gcgattttgt ttctatgcct atggcggaaa
     8461 tcgaacgctt ttctaagatg gaacagttgt tagaaagtaa attatccaga cgctttcaga
     8521 ttcgtaaatt ggagaaagat gattttggtt atattattga acatctttac gggcaaacag
     8581 gtgtcccttt tgatgactac gactaccatt taccgaaacg atacgaagat aaaaaagtgt
     8641 tggtaaagaa atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct
     8701 atttacggat tgaacaggaa gataccacta cttatgtggc gtattttaca attaatagta
     8761 ttgttggtga attggatttc cctaatagtg aaatttttta ttaccagcaa cagcagttta
     8821 ctttccccgt tgataccagt atgaatgttg aaattgtcgc caataaaaaa gccttatcta
     8881 ccgttcgcaa taaaaagaaa gaactgaaag atttagatag ccacgcatgg gaaagcgaca
     8941 acgaaaccag tactaatgtg gtggacgctt tagaatctgt cgatgaatta gaaagtaatt
     9001 tagaccaaac caaagaatct atgtacaaac taagctatgt ggtacgggta tcagcaaaga
     9061 atttggacga gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat gatctgtcta
     9121 tcaaattagt acgacctttt ggcgatatgc taggcttgca taatgaattt attccctcaa
     9181 gcaagcgata tatcaatgac tatattcaat atgtcacgtc ggattttttg gctggtcttg
     9241 gtttcggtgc tacacaaatg attggtgaaa attctggtat ttacgtcggt tacaatctcg
     9301 atacaggcag aaatatttat ctccaaccta gccttgcaag tcaaggaatt aaaggctctg
     9361 taacaaatgc attagcgtcc gcttttattg gttcattagg tggcggaaaa tcattctcta
     9421 acaatatgct ggtctattat tcagtactct ttggcggaca ggcaaccatt gttgacccaa
     9481 aagctgaacg tggtgaatgg aaagaaacct tacctgatat tgcccatgaa atcaatattg
     9541 ttaatctcac gagtgagaac gacaataaag ggttacttga cccttatgtg attttaaaac
     9601 tcccgaaaga ttcagaaagc cttgccattg atattctcac gttcttaaca ggtatttcca
     9661 gccgagatag cgagaagttt cctgtcctgc gtaaagctat tcggagtgta actcaaagca
     9721 aacaacgagg gttgttattg gtgattgatg aattgcgaaa agaagatacg cccatcagca
     9781 atagtattgc tgaccatatc gaaagctttg tcgattatga ttttgcccat ctgttattca
     9841 gtgatggcag tgtcacccaa tccattagct tggaaaaaca actcaatatt attcaagtgg
     9901 cagatttggt cttacccgat tccaatagct cttttgaaga atacactacc atggaattat
     9961 taagtgtggc aatgctgatt gtcattagta cctttgcttt agactttatc cactctgacc
    10021 gttccatttt taaaattgtt gaccttgatg aggcttggag ttttttacaa gtggcacaag
    10081 gaaaagctct atctatgcga ttggttcgag caggacgtgc catgaatgca ggggtctatt
    10141 tcgttactca aaacgcggac gatttactag atgaaaagat gaagaataat attggcttga
    10201 aatttgcctt tcgttccact gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg
    10261 tggacaagga agatgaaaac aatcagaaac gcttacgtga attagaaaat ggacaatgtt
    10321 tagtttcaga catctatggt cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt
    10381 tgcttcatgc ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa
    10441 acaaaagaaa tttcttcact atagttggat aacgctcctt tgtgtgggcg ttattttact
    10501 tttattaggc acgctgggca atgcagtaca agcgacaggc ttagttgatg aaaccattga
    10561 tacaagcaat gaatactcca agtatggttt gaatcaatat cagcttgatt attatgtgga
    10621 caatagctgg ggctggcttc catggaattg gagtgatggc ataggacaat cggtgatgta
    10681 tggattgtat gccattacta attttatttg gacaatttct ctttatctat cgaacgccac
    10741 aggttatttg gtgaaagaag cctatgcctt agatttcatt agtcaaacat cccaagcaat
    10801 tggcgaaaac atgcagacga ttgcaggcat tacaccgaat ggcttttcca gttcagggtt
    10861 ttatgtcggt ttcctccttc tttttattct cattgttgga atctatgttg cctatacagg
    10921 attggttaag cgagaaacga cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt
    10981 tattctatcg gcttccttta ttgcgtatgc acccgattat attgcgaaaa tcaatgattt
    11041 ttccaaagat gtcagtaccg ccagtctgaa tattgggaca aaaattgttc ttcctaacac
    11101 ggatagtcaa gggaaagaca gtgtggacat gatacgtgac agcttattct ctgttcaagt
    11161 aaagcaacca tggctattgc ttcaatatgg ctccactgat gtagaagcgt taggggaaga
    11221 acgtgtggaa agtttattgt ctaccagtcc agatacaaac aatggtgaag atagagaaaa
    11281 tgtagtgatt gaagaaattg aagatcatga caatacctat ttaaccctac cgaagacgat
    11341 ttccagatta ggaacagtct tctttttgtt tgtattcaat attggtatct cgatttttgt
    11401 tttccttcta acaggaatca tgattttttc gcaagtgcta tttattattt tcgctatgtt
    11461 tttgccaatc agtttcctat taagtatgtt gccgagtttt gatggcatgg gcaggcatgc
    11521 cattaccaag ttatttaatg tgattatgac aagggctggt attaccttga ttattaccgt
    11581 tgcctttagt atttccacta tgctttattc gctgtcagca agttctccct ttttcatgat
    11641 tatgttttta caaattgtga cctttgcagg gatttatttt aaactgggcg atttaatgag
    11701 cctattcact ttacaaagca acgactctca aaacatgggc agacaagtgt ttcgcagacc
    11761 tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg
    11821 tggaaaagca gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa
    11881 agctaaccat actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa
    11941 tgtgaaaaag caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt
    12001 ggacacgaaa aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac
    12061 accaacccat gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac
    12121 agactttaaa caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca
    12181 gcaggagcaa aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga
    12241 aaaaagacga aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa
    12301 gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg
    12361 accattgaaa aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct
    12421 aaataaaaga acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt
    12481 agtgtggtct tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat
    12541 gatacggaca gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt
    12601 tcaccagaag tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt
    12661 gaagactatg tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa
    12721 gatgttatgc aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag
    12781 tccatcaaac aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga
    12841 gtggatatag attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg
    12901 aggagtcatg ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg
    12961 ggtggtcaaa aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga
    13021 tataactatg gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc
    13081 ccaacagaat ttgatgatga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa
    13141 gggtttccct atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta
    13201 atacaatggg tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat
    13261 gacgctacgc aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat
    13321 tcaacctata atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc
    13381 ttttatcatg caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac
    13441 catctgattg gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat
    13501 aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca
    13561 aagaaaagtt ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt
    13621 tttattgatt ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca
    13681 tactgtccgt gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag
    13741 ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga
    13801 taaacgaaat gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga
    13861 aatgattcgt aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt
    13921 ttctcaagtg aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga
    13981 agacaaagac aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc
    14041 agataatatg gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta
    14101 ccaacccaaa cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac
    14161 tagcttttta gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta
    14221 ctatgtaagt aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt
    14281 gaatcctatc tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt
    14341 agatcaagaa acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga
    14401 taattggaag attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat
    14461 gtactctttt tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat
    14521 ttaagtgact atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt
    14581 caatttgata gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt
    14641 acccagttta agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc
    14701 tttgtatacc tatggttatg cataaaaatc ccagtgataa aagtatttat cactgggatt
    14761 tttatgccct tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag
    14821 ttttagctca tgttgatgca ggaaaaacta ccttaacaga aagcttatta tataacagtg
    14881 gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt
    14941 tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata
    15001 cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt
    15061 cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac
    15121 aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca
    15181 ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac
    15241 tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga
    15301 actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga
    15361 aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa agcataagat
    15421 ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaagtaat atagggattg
    15481 ataaccttat agaagttatt actaataaat tttattcatc aacacatcga ggtccgtctg
    15541 aactttgcgg aaatgttttc aaaattgaat at
//
LOCUS       DAYYNG010000060        15510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000060 DAYYNG010000000
VERSION     DAYYNG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.060"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1383
                     /locus_tag="ACHBFA_001505"
     CDS             103..1383
                     /locus_tag="ACHBFA_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9394999.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            1396..2124
                     /locus_tag="ACHBFA_001506"
     CDS             1396..2124
                     /locus_tag="ACHBFA_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9395000.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(2193..3716)
                     /locus_tag="ACHBFA_001507"
     CDS             complement(2193..3716)
                     /locus_tag="ACHBFA_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9395001.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            3968..5149
                     /locus_tag="ACHBFA_001508"
     CDS             3968..5149
                     /locus_tag="ACHBFA_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9395002.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(5209..6837)
                     /gene="treC"
                     /locus_tag="ACHBFA_001509"
     CDS             complement(5209..6837)
                     /gene="treC"
                     /locus_tag="ACHBFA_001509"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9395003.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(7234..9945)
                     /locus_tag="ACHBFA_001510"
     CDS             complement(7234..9945)
                     /locus_tag="ACHBFA_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9395004.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(9955..10656)
                     /locus_tag="ACHBFA_001511"
     CDS             complement(9955..10656)
                     /locus_tag="ACHBFA_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395005.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            10842..11444
                     /locus_tag="ACHBFA_001512"
     CDS             10842..11444
                     /locus_tag="ACHBFA_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9395006.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11525..12538
                     /locus_tag="ACHBFA_001513"
     CDS             11525..12538
                     /locus_tag="ACHBFA_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9395007.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            12647..13564
                     /gene="rbsK"
                     /locus_tag="ACHBFA_001514"
     CDS             12647..13564
                     /gene="rbsK"
                     /locus_tag="ACHBFA_001514"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM9395008.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            13592..13987
                     /gene="rbsD"
                     /locus_tag="ACHBFA_001515"
     CDS             13592..13987
                     /gene="rbsD"
                     /locus_tag="ACHBFA_001515"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM9395009.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            13987..15360
                     /locus_tag="ACHBFA_001516"
     CDS             13987..15360
                     /locus_tag="ACHBFA_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395010.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         4498 a         3029 c         2837 g         5146 t
ORIGIN      
        1 gctgctttgg tatgagttct gaattcaaaa tccctgaaaa attaaaaaaa taatacgcta
       61 attgtgctat aatttggtaa aaatcaatgg gagctgtact ttatgttttt atggtccata
      121 gcgttgcaat atttgattta cacaatttgt atcttattga ttgaccatca catccgatac
      181 aaatatctgt ttgcgggagt cattctgatt tcttgtattt tgattggatg ggtctattcg
      241 attttaggga tttttactgc tctaattgct ataggaatgc tgatgcttac ttttttcttc
      301 ttttcgactt cgcgtcaaca attggtttac gcgcttcctt ttgggttatt gacgtcgctt
      361 ttaggagatc acttaacaag catcggcgat ttttttgttt tgaaatcttc ggttattgaa
      421 ccaggagatt cattgcagta ttttcacatt gtcttatccg cttttttatc tgctttatta
      481 gctttttgtt taaaaaaatc gttagagact tggtttcaca ctgtggaccg ccgtatgatc
      541 ggtacgatcg ggacattgat attatttaca tactatatca ttatttttta tacccgtttt
      601 tctggtgaaa cacctgatgt cttagctttg aatacgagtt ttttcattct atatctgtgt
      661 gttggcctga tcatcttggt ttattattgg aaaatctcta ataagaaatt agctgatcag
      721 ttattagagc aacaagtcag gatgcaacag gattatatca aagatctaga aaaaaactat
      781 caagacttga gagaattcaa acatgattac caaaatctgc ttttttctct tcacagctat
      841 atttcagaag gcgacttaga aggattacgg tcttattatg atcaaacgat attacctaca
      901 cgccagatga tgaacctttt tccggccaat ctaagtatgt tggataatat gaaagttcct
      961 gagatccgta gtttactgtc attaaaactc atgatggcac aggaaaaagg gttgatcgtc
     1021 cagcttcatt ttcctgagcc aatcgaactg gatacgaaac atacggtaaa ccttgtacgt
     1081 atgcttggta ttatcttgga caatgcgatt gaaggagcta gtgctgcaaa gaagaaaaaa
     1141 atcgaattga ccatactcaa gaagaatcat tccgtgatga ttcgtgtgat caatacaaca
     1201 acaaatacat taccgttaaa ccagttgaaa caaaaagggt tctcaacaaa aacaaatcca
     1261 aaaaacgaag gccttggctt gtttatcttg gatgaattga caaagaccaa tccttatctt
     1321 ttccttgaaa catcaattga aaaccagcgt ttttctcaaa cgatttatat acaaataaaa
     1381 taaaggagta atgatatgat tcccatttat atttgtgaag acgatccaaa acaattagaa
     1441 gttttatcaa cggtgatcaa aaatagaatc atgattgaaa acttagacag ttatgtccat
     1501 atttctacct ctgatccgga agaacttctc aatgcagcca agatcagaac ttcttctttt
     1561 ggtatttact ttttagatat cgaactagaa gacagtgaaa tggatggtgt agcgatcggt
     1621 aaattgatcc gtgaattaga tcctttagga aaaataatct atgtgacttc ccatacggaa
     1681 atgtcgatga aaatcttaaa aagcaacatc gaaccgactg attatatcgt caaagaagat
     1741 ctttatgatt taaaagaaaa tgtcgagcat atcctgagca aaattttcga agattttcaa
     1801 atgtcccaaa tccaaaaaga tatcttcaaa atagagttta atgatgagat caaattttta
     1861 ccgatcaaag acatccaata tttttctact tcgctaggag cgccacataa actggaagtc
     1921 catttgactc atgcccaatt acaattctat gagaagatca aagaaatcga gaaaatgcat
     1981 cggaatttta tccgttgtca taaatcctat gttatcaata cacaaaatgt gcggtcgatt
     2041 aataaaaaaa ccagagaagt cacgttggca aacggcgaga ccatcccagc ctctatccgt
     2101 ggattaaaga aattgatttc ataaaaagaa ccgaatagtt tggaccgact aaatgaccag
     2161 ccgatccaaa cccttcggtt cttttttgcc tatcagtgca aaagcatttc ctgtgcaatt
     2221 tcacgtccat ccattgtatc tggataaaat gttggccaat tctccatttc tttcagcaat
     2281 acttcttgac tttcatttcc tgcatataaa tgaaaatgtt cggagtcagc cggtgcaatc
     2341 gtgtgatcac tgaattgaat atatctaaaa gcgccacttt ccttatcttc tgcttcgaat
     2401 agatacctta cgccacggtt tcctgcctcg taggtcaaga tttctttccc tacataacga
     2461 taatttgaac gtttcgttgt tccatcctta aacgtaaatg ccatctcttt ctcactgatc
     2521 ttgattgaag caatatctgt ttgatagccc ttcgtataat attctttata ttcttgtgct
     2581 gtcttatcct gattcaattt ttctttatat tcaaaaacag gatcaaaagt cccatcttct
     2641 acatacgtat ctactgtttg ccaagttcct gcccaatctg aaagagagcg atcttgtaca
     2701 tcctcgtctt caaaataacc gttgtagacg ctcttttctt ttttgggggt caaagtatct
     2761 tctagcgggt tttctgtctc tgtcgttttt ttcaacgctt cgaggtttgc ttccatgata
     2821 gaaagatagt tttctccatt ttctatttgt tcattcgtca atccttccaa aggattcaat
     2881 acttctaaag atacacctgc ttcgtttgct aacgttctgg caatactatc tttggcgttt
     2941 tcttcaaaat aaatatagtt gattccatgt tctttaacaa agtgtttcag ttcagcaatt
     3001 ctcgccgcag acggttcttc actagaagaa agaccagcaa tcgatacttg attcaatcca
     3061 tattctaacg ctagatagtt gaatgcagca tgttgcgtga caaaagatct ttgttttgct
     3121 tgcttaaagg tttcttgata aagctgatgg agtgcttgaa gtttctcggt ataggctgct
     3181 gcattttttg tccatacttc ctgtttttct gggtaagctt caatcagctg atcacgtatg
     3241 tttgctacct gcttgattgc taaacttgga gctagccata catgtgggtc tagctcatgt
     3301 gtatggcctt cttccccgtg ttcatggtca tgatcatgtc cttcttcact tcccggaagt
     3361 aaaagcatac cttctgtcgc ttttatcgta tggacattgc cttcttgtaa tgttttttgg
     3421 attgcgggga cccatgtttc catgttttca tcattataaa caaagatatc tgtatcttgg
     3481 atctttgcca tgtctttagc agaaggttca taatcgtggg aatcagtccc tgctggaatc
     3541 aataactcta cttctccttc atccccgacg attgcttttg taaaatcata catgggataa
     3601 aaggtcgtca tcacgctcaa tttttcttta ctgtcctcgg ctttcccact tgccccgcaa
     3661 gcagttaaca tacctgctaa aaccagcatg gccaatatac ctatcaatcg tttcatttat
     3721 gacactcctc ttctcatctt ctttcatttg aatgttccga ttcgcttttc gcaaaccgta
     3781 atagttacgt tttagcaaca ggatttaatt tatcatatgg ttaaaagaac tgtcaagcgt
     3841 tttcttattt taattgatga gaaagtttat atgaatgatt gacgatattt ttgcaatcaa
     3901 cctctatcat tttcctttta tacccgcttt tgtctataat gaagttatag ataaaggagg
     3961 aaaaagagtg aaaaaagaaa cagatacaac gaatcgtttg atccgctttt taggaggaaa
     4021 aacctcttac tatgttttag gaatagttat cttacttgcg ctgatcattt attttttcca
     4081 tcagatctcg tttatattca agcctgtcgg aatcattatc gctactatct tgcccccatt
     4141 attgtttgca ctgatcttgt attatctttt ggaaccagtc atcaaacgtt tgagcaagca
     4201 cctttcgagg aacttgtcag tcattatcgt ttatgtgatt attttatttt tattggggct
     4261 tggaagcata tggcttattc cttttcttga agaacaggca aagacattga tcagtagttt
     4321 gccagatttg tttcaagatt ttcaagagag tctccgacaa tttttggaga aaacaccttt
     4381 tgccgattca tttaatcaat ttttcgcttc tattgacgac gtgacgtctg atatagctgg
     4441 atttattgga aattattggg aaagtggcgc acaacggatc agcaacattt tttcaactgt
     4501 tacagctatt tttattactt tatttactgg tccaattgtc gcttttttcc ttttgagaga
     4561 tcctcaaaaa ttttatcagt cagttttagc gattgtccca ccagcatttc gtacagattt
     4621 taaaaatttg acaaagattg ctaatcaaca attgggttca tttctaaagg ggcaaatcat
     4681 tgcttcattt atccttggag cagtctactg ggtttgtttc ttactgatcg gattagaatt
     4741 tgctagtgtt ttagctattt ctgctgggtt gttatgtatc attccatata ttgggccgtt
     4801 tatcgccttc tttccaggtt tgatcattgc attccaagac tctactttta tggctgtcaa
     4861 atttgtcatt gtctggtttg ccgtacaact tctccacggt gacttggtga ttcctcgagt
     4921 aatgggtgat cgtttacaga tccatccgat tacgatctta atcgttttac ttgtcatggg
     4981 tgatctaatg ggaattgtgg gcgttatttt tggtattcct atctatacgt tagtcaaatt
     5041 actcgttact tttattttcc gtaaattcaa acaacgctac aataaatttt atggcgataa
     5101 aggagagtat gaaaactctt cattttctga agaagattat tttaaataaa acacaaaacg
     5161 tcgatcttac gagaatagct cgtaggaacg acgttttgtg tttttgtttt aatttttcag
     5221 caaagcaatt gcctggtatg gttttagctc cagactttga tgaagaacag tatcggaata
     5281 attatcgata agaatattgg cttctgtgaa tccttctggg atggcgatcc ttgtatcttt
     5341 atcatagaaa tttgttaata caagcagctg ctgtccttca aattggcgaa gaaagccata
     5401 aacttccgga tgatttgctt cataagcatg ataatctcct tgtgcaacga ttggatatgt
     5461 ttttctcagc cgaatcaatt tcttgtaata ggacaaaata gaatcttcag aagatagttc
     5521 tttttctaca ttaatctctt ctagcttgcc tccaactttt aaccaaggtt tccctgtagt
     5581 aaatcctgct tgttctgtgt ctgaccattg catgggtgtt cgagagttat cgcgagattt
     5641 tgctttgatc cgacgaaacg cttctttttc tgtgaatcct tgttcaagga gtaacttata
     5701 agcattcaaa ctttctacgt caatatagtc ggaaatcgat gaatagtctg ggtcgatcat
     5761 gccgatttct tctcccatat aaatataggg cgtcccacgg ttcaaatgga tcatggttgc
     5821 tagcatctct gctccttgtt ttcgatactg atcgatagct acaaatcgat tgagcgcacg
     5881 tggctgatca tgattattaa aaaatagagc attccagccg ttaccctcac tcatcttttc
     5941 tccccatgta tgaaatagtc gctttaattc tgcaaaatca aatggaggag tagaccattt
     6001 attcccattt tcataatcga ctttcaaatg atggaaatta aagaccattg ataattcttc
     6061 tctactaggt tctgtataca agatacaatt ttcgatcgtc gtagagctca tttcaccgac
     6121 tgtgatgata gtggaatccg tcccaaacgt tgcttggttc aattctttta gatacgtatg
     6181 ggtgattggt ttgtccgtat attcaggttt gccatcattt tctttattat ttttcaattg
     6241 ttcgtcttta ccgataacat tgataacatc gaaacggaat ccttttaccc ctttttccat
     6301 ccaaaaacga acaactttga atagttcctc tcttactgct tggttccgcc agttcaagtc
     6361 agcttgtgtc cgatcatata agtgcaaata atatttcccc gtatctccaa atggcgacca
     6421 agcacttcca ccaaattttg atacccaatc cgttggctct tctcttaaaa tgaagaagtc
     6481 ctgataatgc tggtcccctg ctagtgcttt tttgaaccat tcatgttctg tcgaaacgtg
     6541 attcagcacc atatcgagca tgatttctat ccggtatttc tttgcttcgg ctattagctg
     6601 ttcgaattcc tccatcgtac caaagatagg atcgatggct gtgtaatcag agatatcata
     6661 accgttgtct ttttgagggc ttggataaat agggtttagc caaatcatgt cgatgcctag
     6721 atcattcaga tacgggagtt tttctttgat tccttttaaa tctccaattc catcaccatt
     6781 actgtctttg taagatttgg gatagatttg gtaaatgact ttgtctttaa atgtcatgga
     6841 tatttacctc cattcaactg atttattacc taccacaact tatatataca agttatcatt
     6901 tattattata actgaaccga aagcgtttgt aaaatttatc tttatacatt ctaagaggtc
     6961 tgttgcccaa taaaaaacaa gagatcagac atgtatttgt ctatcctctt gtttaaaata
     7021 gcaaaatatt cttgtttctt ctataaatgg tgttcaaaag cttttatcta cggcaatcag
     7081 tcttctcaaa tgtaaaacat gaggagctaa ttttatttga gtattctttt tgctcgatgc
     7141 aggacggctt ttttacaacc tctgattcat ggtgttcaat tagcagctga ccctcgttat
     7201 tagctcaaaa cgctgatttc acaacctctg tttttattca ttcgatttca acgcatcaat
     7261 catatcgata tgctttaatt tgaaatgggt aacaatcatc acaataaccg taaatacgat
     7321 agttaatcct gctgataaga cataagccca ccatgtgatc actaatggaa agatgacatt
     7381 ctccatagac gcttgttgca agatgaagtc agtcaatata tatcctacac caaatcctcc
     7441 gatgattcct aaaagagtaa agatgatatt ttctcgaaca atgtacatcg tgacttcttt
     7501 atcaaaaaat ccaaggactt ttattgtaga taattcgcgt acacgctcgg aaatattgat
     7561 attagttaga ttatacaaga cgataaaagc cagcagtcct gataatacga caaagattaa
     7621 gacaaccgag tctaagttcg ccattgattc ttcttgcgtt tcgatttgtt tggacatgaa
     7681 agacgtattt gtcacagcgt ctgtggcaag cagtttctcg gaaagcattt cttcttcttt
     7741 tgttgtcatc ttatctgtac gcagcaaaaa tgcattatcc atcatcgatt gatcagtaac
     7801 tgaagcataa tattcagggg acatataaat aaagctacct agatagtttt tcacgatttc
     7861 ttttacttcg actttccatt ctttttgatc ttcatcataa agcgaaatat gatctcctgt
     7921 agataagtca aatagttctg ctactttttg cgtaatagcg attcctgttt taggcaagga
     7981 gcgtgggttc tcttcaagat cagttaaatg gataaaacga tccaaatcag ctgttttact
     8041 tggtacgatc actgtcagac tttgcgaagc atgtcctttt tttctcaatt ctacggtttg
     8101 agtatgaata gctaggatct catttacttt ttcttgcttc tgcagcatat ccgctgcttt
     8161 ttgacgacta tcttccgtgg atgaatccaa tgtaacgatt gcttgatagt caatgatcgg
     8221 tccaaattgt ttggctgaaa cagaacttag agaatctttc aatcccacgc ctgcaaccat
     8281 cagcccggca catccagcaa ttccgataat cgccataagc attcttgact tatatcggaa
     8341 gagatttcgg taactgattt tttgattaaa gctaaggcgt gaccacagcg gagtgatgta
     8401 ttcaagaaaa atgcgtttcc ctggcttcgg tgctttcggt tgaagcaatg cagcaggccg
     8461 ttctcttaaa tctttgaata aaacgatcat tgcagcgcca aatgcggcaa tgaagaatgc
     8521 ggcagctgct tgtaaaatcg gcggccagtc ataaaaaata agcgcactcc caatatcata
     8581 ccgctcgtta gacagctcaa aaatgatcct tggcaaaaga ttcgttccta atatcgttcc
     8641 taaaacaata cctatccctg aggctagact tgcatagatt gcatattttt ttgcgatttc
     8701 aactttcgta taacctaacg cctttaatgt cccgatttcc ctgcggtttt cttctaccat
     8761 acgtgtcatt gtcgtaaagg tgatcaatgc ggcaataaag aaaaagaaaa caggaaaaac
     8821 attggcaatc gcagcgatac gatcagacag atcaccaaat tcttgaaaac cgacattttc
     8881 tgaacgttca ttgtataggt atgatggttt agttaaattt tcaatttttt ctgcatttcg
     8941 attcaattct tcctctgcag aggccagttc acgttctttc tcaactaatt gcctttcttg
     9001 ttcagcaagt tgagcttctt gttgagataa tgtttgaaca gcgattgttc gttgcgcttc
     9061 aggaagctgc tggatagttg ctgactgttg cgctaactgt tttttagcat cttctaaccg
     9121 tgttcttgct tgcgttgttt gtgctttgcc atccgctatt ttttgctgtg ccggttctaa
     9181 ttcttcgttg gccgattttt tcagctcttc taagcgttct tcgggtcgat cttgtaaata
     9241 cttttctgcc ttttcttgat tttgctccat tttatctttg taagctttgc tgtacgtatt
     9301 caaattcttt acgtttgtaa aatcaagata taagacagaa taaatatctg ttttaaaaac
     9361 ggattcaggt aaatagacaa aataatcgac gctaccattt ccgacgttcg cgtatcctct
     9421 ctccaaattg ttgatgaaca atggagaacg gacaaagccc gtgatggtat actctttttc
     9481 tttcaattca tctgaatcaa tctgataaat atcaccgatt ttataccctt catcttccgc
     9541 tttttcatcc aatacaagct ggtttggttt ttccgggagt tttccatcag ttaattgtag
     9601 cttgttctgc ttgttatttt tgttataaga aaatatttga atcacttcgt ttttgccagt
     9661 attggaataa tagatctgtt ttcctgtttc tacttttgca tctgggatct tttctgcttg
     9721 agcaatatct ttttgtgtca agccaccagt agaaacgatt tgcaaatcag aaagtcctgc
     9781 tgtaccaaca tagtgatcca tcgttttttg catgacaggt ccagcagttt tgacacctac
     9841 aaataaaagt gttcctagca tgacaataag gatgattgca atgaatcgtc ctttagattg
     9901 ccgaatttcg cgaaaacctg ctttaagata tgttcttgtt ttcataaaat ccccctacca
     9961 tacaatctca tcaatggatg aaggatgatc atttaattca acagacctta ctttagcatc
    10021 attaatgtga atgactctat cagcgatggg cgctaaagca gaattatgtg taatgatcaa
    10081 aacggtattg ccgtgttgtc tgctagcgtt ttgcaataat ttcaatactt gcttgcccgt
    10141 ttcaaaatcc agcgcgcctg tcggttcatc acataaaaga agtttgggat ttttagctag
    10201 tgcacgagca attgaaaccc tctgctgttc gcctcctgat aattgggacg gaaagttatt
    10261 caatcgatga gctaggccca cttgcctcaa tacctctaca ggatcaagcg catcaggaga
    10321 aacttccgtt gccaattcca cattttcttt tgctgttaga ttaggaacga gattgtagaa
    10381 ttggaaaacg aaccctacat ctgttcgtcg gtaagcagta agctgtttat ctgaaaattg
    10441 cgcaatatct gtgtcatcaa taatgatctg accttcatca ggtgtatcca tcccacctaa
    10501 aatattaaga atcgttgact ttcctgctcc acttggacca agtataatca ctaattcgcc
    10561 tttttcaatt gaaaaagaaa tatcgtcatt tgctgtaatg gtcgtttctc ccattttgta
    10621 tcgtttgtat tcatttttta cttcgacata cttcactctg attcctccat tttcacgata
    10681 tttctcgcta tatctatgca tcgaatgcgt tttctcacac ttatttattt taccactgaa
    10741 tttctctaga ctaaagaaag gaatccttta atttagttga caaataaacg atttatgatt
    10801 tacttacata aaaaacattg ttatttgaaa gaaggaacat catgaatgat ttaaaggaaa
    10861 atatgatttt tgatttatca aaaactgctt ttgtcgcgat cgacatgcaa aaagggatcg
    10921 taaataacgg tacactctct ccacacactg cacaatctat tctgacaaac aatgatttgt
    10981 tagcaaaaac attgaaacgt accgctgcct tgaaagtttt agtgaatgta gacatcacta
    11041 ctttctctta tctaagtcat cagacagatg ctggaggaat atctgctttg tctgctccgg
    11101 cagaattcac tgacttgttg ttgaaggatt ccttgaaaga tacaaacaat atgttgacta
    11161 tcacaaaata caatccaagt gccttttttg gtacttctct tgatcttcaa cttcgccggc
    11221 gtggaatcga aactatcgtt ttaagcggtg tagcgacaac aaacggtgta tatgctactg
    11281 cattagatgc cttccaacat gggtatcatg ttgtacttgc tgaagatgct tgcagtgatc
    11341 gtgacaaaga atcccatcaa ttgttcataa aaaagatatt tccgaaaact gcacgagtac
    11401 gctcaacgaa acaaataatc gaagctatcc aacaaagcaa ataagtactg gcacacgtga
    11461 ttttcattta cttctttttg atttcatggg aaaataacca caggatttag ataggagtat
    11521 gaacatggtg aaaaaaaaga tcacaatcaa agaagtcgct aaactttccg gcgtttcaat
    11581 cacaactgtc tccttgatat taaatggaaa tacggagaaa ttcagtccga aaactgttca
    11641 gaaagtttta gctgccaaag aagaattagg ctatcagcct gactattttg ctagacaaat
    11701 gattaccaag gaaacgaaaa caattggtgt actcgttcct gatatcacga atccattctt
    11761 cagtacactg atgcgaggaa ttgaggatat cttgtataag cagaatttcg tcacgatatt
    11821 atgtaatgct gattctgatc atcaaaaaga aatcgaatat ctagccgagc tcacacgtcg
    11881 aggtgtggat ggattcatta tcgcgacttc tgctgtctct acagatgcaa tcaacgaaaa
    11941 tttgaaaaaa caaggccggc ccttcattgt tttggatcag aaaaaatctg aaggatttag
    12001 tgatgccgta cgaacagacg attttcgcgg tggttaccta gcgggcatgc acttgcttag
    12061 tctaggtcat caaacgatcg cccttgtcta tcctgaaaat ccgccggaaa atgtccacgc
    12121 tcggattgag gggtttaaat ctgctcttga tgtttaccaa atctctcacg accaactgat
    12181 cctcttacct acacagttct caaaacaagg tggctaccaa atcacagcag aacttcttga
    12241 ttctgctgca actggtgtgt ttgctttaaa tgatgaatta gcttttggat tatatcgcgg
    12301 attagaggaa tctggaaaaa gcattcctga agactacagc attattggct atgataatat
    12361 agatatgtgc gagtatatca agccgaaact gacaacgatc gcacaaccga tcttcgaatt
    12421 gggacaaaca tctgccaaat tattgttaga tcggatccaa ttcccagaaa aagaatggga
    12481 agaaaaaaga ttacctgtac gattagaaaa aagattttca actgcaccct tgaaatagct
    12541 ttcaaggtgt gctatatttt tatcgaacta ttaaaacgtt ttactaaaac gttttaaaaa
    12601 ataatttttc ccatcaaaat aaatgagata gttggaggtt tgtaaaatga acacggttac
    12661 agtaattgga agtattaata tggacactac ccttcgttta accaacatgc ccaagccagg
    12721 cgaaacaatg catgcgcatg agatttttca cgctggcgga ggtaaaggtg ccaatcaagc
    12781 agtggcagcc aaacgttcag gcgcacggac atcttttatt ggaggcgttg gggctgacag
    12841 cgaaggacaa cagttattag atctactgac aaaagaaaac atcgatacat ctgggatagc
    12901 tgaaatccaa ggcgccacaa ccggacaagc aatgattatg gtagacgcag caggagaaaa
    12961 tagtatcttg atccatgcag gtgcaaataa tgctttccat gaacaagaag tcctaaaaaa
    13021 caagcaactg attacaaaca gtgactttat tattgctcag tttgaaagta gcttagatgc
    13081 gacgatccta gctttttcta ttgcaaaaga cgcaggaaaa acaacgatcc tgaatcctgc
    13141 accagctcgt gaaacgatcc ctactgaact acttgaaaaa acagatatca ttataccaaa
    13201 tgaaacagaa acagaaatca ttacaggaat cagagtgaca gatcataaca gtttggtcgc
    13261 agcagccgaa aaactccatg aattaggtat tggaacagta atcattacac taggcagtgc
    13321 cggcgctttc tatcatacag aaaaagaaca cggtatcgtc cctgctttta aagtggatgc
    13381 cgttgacaca acagccgcag gtgatacatt tattggcgca ttaagcagca cacttcagcc
    13441 agaccttagt aacttgaaag aagcgatcct ttatggaaat ctagcctctt ctgttgctgt
    13501 gcagtcttat ggtgcgcaac cttctatccc ttaccgcgaa gctttagaac aatcagcagt
    13561 ctaaaattaa caatgagagg aagaaacaaa catgaaaaaa ggaaaagtaa tcaattcaga
    13621 tatttcacga gtaatcgcac aaatggggca ctttgataaa ttaagtatcg gggatgcagg
    13681 catgcctgtc ccctttggta ctgaaaaaat agatttggct gtctcaaacg gtcttccaag
    13741 ctttttatct gttttagaaa acgttttaga agaacttgag gtacaatcga ttgaattggc
    13801 ggaagaaata aagacaatga acccggaaat attggaaaaa atcaaattat tgttaccaga
    13861 tacaccgatt agctttatcc cacatgaaga aatgaaaaaa gagttgaata actgtcatgc
    13921 atttattcgt acaggcgaaa tgacacctta cgccaatatt atattagtga gtggagtggt
    13981 cttctaatgg aaacgacaat gaatcctgaa cagatggtac tgtccgagca gattcatatg
    14041 ctgattgaac agttgggagg aaaagacaat attgattctg tccttccatg tgcgactcgg
    14101 cttcgtatca aattgaaaaa acagccagta gccaatgcag aaaaattaaa acagcaaatc
    14161 ccttccatta agtatttcat tttactcgat agatatgcag aaataatcat ggatcaggga
    14221 ttggctcctg cttacaagga attaacgcgt caaatcgatc attcagctcc taaagaaaag
    14281 ctaaatccga tcgaaaaact atttggtttg atatcggaaa tctttacgcc gatcattccg
    14341 ctgtttgccg gttctggtat attaaaaggt ctggttgttt tagcgactac gattggctgg
    14401 cttagtgaaa caagcggaac ttatcatatt ttgtctgctg cgtcgaatgc cgtcttttat
    14461 tttctgccaa tctttcttgc tttttctgcc gccaaaacgt ttaaagtgaa tggatatatt
    14521 gcggctgtca ttgctgcagc actgattgaa ccgaatctga cctctttact ttcagacagt
    14581 ggcaaagcag ctactgattt tctggggatt cctgtagtac tgatgcagta tacgtctact
    14641 gtcatgccgg cgattttagg tattttcttt tattcttttg ttgaaaaatt cctcaaaaaa
    14701 tatattcgcg aaaatatgca attagtattt gtacctttac tttcattagt tatcacggtt
    14761 cctttgacgt taatggtatt cgggcctgcg ggtgtttacc ttggcgaagg tttagctgcc
    14821 gcaattactt ggatgatgga tattaacgga ccgatcagcg gcgcaatcat cgctggtggt
    14881 tggaatattt tggtaatatt tggtatccaa tgggctgtaa accctgtcat gatcagtaat
    14941 atctctgctt atgggttcga ccgaatcgtt ccgttgacag gagcagccaa ttttggaatg
    15001 gctggtgcag ctttaggtgt atttctacga agtaaacgtt caaaaactcg ttcgatctcc
    15061 ggttctgctt ttgcttcgat tttacttgcg ggagtgaccg aaccaactgt ttacggaatt
    15121 gcgatcccat tgaaaaaacc atttgttgct gcttgcattg gtgcagctgc tggtggagct
    15181 gtaatgggat ttgcccaagt gaaagcgatc gcttttgtat ttggtagtct gaccacattg
    15241 cctgcattca tttccggtac ttttttctgg tacttagcag gactggctgt tagtctagtc
    15301 gtagcaatga ttactactct cgttagcggg tttgatgaag acctaatgtc ttatgaatag
    15361 ttaatgaaaa agactgaagt atgagatgtt tatcacatat ttcagtcttt ttctttttag
    15421 gatcattatt tttataaatt tttgtgatac tttccaaact ttggtataat ttacatatgc
    15481 ataaccattt ttatccccac ttgtttatcc
//
LOCUS       DAYYNG010000061        15488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000061 DAYYNG010000000
VERSION     DAYYNG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.061"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1588)
                     /locus_tag="ACHBFA_001517"
     CDS             complement(83..1588)
                     /locus_tag="ACHBFA_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9395011.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1585..2271)
                     /locus_tag="ACHBFA_001518"
     CDS             complement(1585..2271)
                     /locus_tag="ACHBFA_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9395012.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2464..3885)
                     /gene="gndA"
                     /locus_tag="ACHBFA_001519"
     CDS             complement(2464..3885)
                     /gene="gndA"
                     /locus_tag="ACHBFA_001519"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9395013.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4055..4234)
                     /gene="rpmF"
                     /locus_tag="ACHBFA_001520"
     CDS             complement(4055..4234)
                     /gene="rpmF"
                     /locus_tag="ACHBFA_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9395014.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4292..4861)
                     /locus_tag="ACHBFA_001521"
     CDS             complement(4292..4861)
                     /locus_tag="ACHBFA_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9395015.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5047..5949
                     /locus_tag="ACHBFA_001522"
     CDS             5047..5949
                     /locus_tag="ACHBFA_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9395016.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5984..7768)
                     /gene="pyk"
                     /locus_tag="ACHBFA_001523"
     CDS             complement(5984..7768)
                     /gene="pyk"
                     /locus_tag="ACHBFA_001523"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9395017.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7842..8804)
                     /gene="pfkA"
                     /locus_tag="ACHBFA_001524"
     CDS             complement(7842..8804)
                     /gene="pfkA"
                     /locus_tag="ACHBFA_001524"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9395018.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8972..12286)
                     /gene="dnaE"
                     /locus_tag="ACHBFA_001525"
     CDS             complement(8972..12286)
                     /gene="dnaE"
                     /locus_tag="ACHBFA_001525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9395019.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12517..12702
                     /locus_tag="ACHBFA_001526"
     CDS             12517..12702
                     /locus_tag="ACHBFA_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM9395020.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12764..13960)
                     /locus_tag="ACHBFA_001527"
     CDS             complement(12764..13960)
                     /locus_tag="ACHBFA_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9395021.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14130..>15488)
                     /locus_tag="ACHBFA_001528"
     CDS             complement(14130..>15488)
                     /locus_tag="ACHBFA_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9395022.1"
                     /translation="AMSYILFVNPTILSASGMPFQAVFLATIIASIIGTLVMGLFANV
                     PYAQAPGMGLNAFFTFTVVFGLGYSWQQALAMVFICGLINIFITVTNIRKMIIRAIPE
                     SLQHAIGGGIGIFVAYVGIKNAGFLSFSADQSAISSSVVEGGKATNVTINGGIVPALA
                     NFDNAPILLAVIGLVLTAILVVKNVRGAILIGIVATTVLGIFMGVVDLSSIDWQTNSL
                     GNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQVLMTIIAFSLSDTFDTIGTFIGTGRR
                     TGIFSKEDEIALEDGRGFKTKMDKALFADAIATSVGAIFGTSNTTTYVESAAGIGAGG
                     RTGLTSVVVAVLFALSSLFSPLIAIVPNQATAPALILVGVMMMASFADIKWLDMEEAL
                     PAFFASIFMGLCYSISYGIAAGFIFYTIVKVIKGKYNQVSVALWIVDILFIINFIILA
                     TI"
BASE COUNT         4484 a         3308 c         2716 g         4980 t
ORIGIN      
        1 gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa
       61 aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc
      121 taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa
      181 ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt
      241 tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc
      301 caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt
      361 cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa
      421 agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca
      481 catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt
      541 tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct
      601 cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta
      661 atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg
      721 caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat
      781 acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat
      841 ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg
      901 tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac
      961 tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa
     1021 tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac
     1081 ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg
     1141 ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca
     1201 agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa
     1261 aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc
     1321 cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg
     1381 agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg
     1441 ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga
     1501 atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc
     1561 ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt
     1621 ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac
     1681 gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc
     1741 acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt
     1801 tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa
     1861 atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg
     1921 acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta
     1981 gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat
     2041 cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa
     2101 ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa
     2161 accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc
     2221 gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca tttattattt
     2281 tatcctttct attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa
     2341 aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag tctactcata
     2401 acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga
     2461 ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata
     2521 ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc
     2581 tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg
     2641 gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa
     2701 gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt
     2761 ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa
     2821 tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata
     2881 actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc
     2941 tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc
     3001 tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc
     3061 taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag
     3121 gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc
     3181 agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc
     3241 catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag
     3301 ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt
     3361 tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa
     3421 gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt
     3481 taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc
     3541 taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat
     3601 atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc
     3661 tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt
     3721 atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc
     3781 tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt
     3841 acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac
     3901 atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa
     3961 agaacaacat attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta
     4021 gccttgaggt tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct
     4081 taccatcata gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt
     4141 ttggacattc gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt
     4201 tagcttttga tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt
     4261 gtatctaaat tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag
     4321 aatcaaaaag ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca
     4381 tttttctgcg ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt
     4441 ctgttgctgc ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt
     4501 cttcaatcga ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag
     4561 acatttgttc atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa
     4621 aatccatggg cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct
     4681 gcacagtgta atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca
     4741 ctggcgcaac atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg
     4801 tctcatgaaa agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca
     4861 tttttcatca ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg
     4921 aaattttctt gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc
     4981 tgtttgctat catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg
     5041 atcttcatga acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt
     5101 acgaaagtgg ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt
     5161 cttgaaaaag agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattgatc
     5221 gtcacgcctt ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct
     5281 actgcaagag ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct
     5341 attatcggca atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg
     5401 aatcaaattg agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg
     5461 aggatcgatt tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca
     5521 gagttacttt tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag
     5581 caggatactg tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat
     5641 tatgtccacc ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt
     5701 gtttatcaaa gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc
     5761 ggttttttgg cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca
     5821 gatgaagttc agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg
     5881 cctcttcaag aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc
     5941 tcttcgtaaa agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt
     6001 cgccacggta aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg
     6061 ttgttgttgc gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg
     6121 cagcgtgaga agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag
     6181 gcatatattc tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta
     6241 cagcttcttc agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc
     6301 cttgagcttc taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc
     6361 gttcaccaac tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta
     6421 attcaacagc ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag
     6481 caactgttgg gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag
     6541 taacagccaa gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg
     6601 attcagttgc agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata
     6661 aaccgattgt ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt
     6721 ctgataatgc attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg
     6781 ggtagtcccc gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag
     6841 cgttagcaac gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta
     6901 acatttgtgt agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt
     6961 tttgaaccat tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca
     7021 taacaccgtc agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga
     7081 ttttagggaa gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta
     7141 gaacgtcttg tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac
     7201 caaaacgaat atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg
     7261 gtgcatttac ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt
     7321 cacggtttgc ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa
     7381 tgtgtccgcc aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt
     7441 ttgaacctac atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga
     7501 tgtatcctgc acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag
     7561 ctccttttgt atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga
     7621 ccatagtcat acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga
     7681 catttgcccc agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta
     7741 atgtactaac gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt
     7801 taaagctgta cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta
     7861 tttagatcat atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg
     7921 tcagacgcaa cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga
     7981 agcaattcga cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt
     8041 gctccaccac ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct
     8101 gaaagtttct cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa
     8161 tgtttcttcc cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa
     8221 tcatgttctg ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg
     8281 tctcctgcat tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg
     8341 tcacgaatac ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg
     8401 aaatcagtcc caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg
     8461 cgttttgtca aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct
     8521 tcgattccga attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat
     8581 tctggataac gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct
     8641 gccacatcaa gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg
     8701 tatacttcca gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt
     8761 ccaggtgcgt ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc
     8821 attaaaatac tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga
     8881 ctaacattct aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa
     8941 aatgaaagca agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa
     9001 gcagataaat aagttgccgc tttatactct tgctgtcatc cacccaataa gcatctgaca
     9061 gaactacttt tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt
     9121 tctgaaaaat cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac
     9181 gcaaatagca agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt
     9241 ctccaattag ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga
     9301 tcactcgatc aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga
     9361 cagaaatttc tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg
     9421 tccgaatttc tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga
     9481 tatctgtaat cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat
     9541 gacctgacaa ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat
     9601 agtcagcgac ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac
     9661 tgtaaacgat attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag
     9721 ataattcatc aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac
     9781 gcttatcgat ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt
     9841 cgtttaatat atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc
     9901 tgatttcttc cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg
     9961 aaagtttgag tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat
    10021 gccgaacaga attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg
    10081 ccatttggaa tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg
    10141 caaatttttc gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta
    10201 atgctcctga tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca
    10261 ctgcccgtcg cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct
    10321 gcatgatttg ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg
    10381 aatcatctgg gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat
    10441 tttgcatagg gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg
    10501 gatctaatct tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg
    10561 tttcgcctcg ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc
    10621 ggatctcata cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt
    10681 tgcgtagtcc taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat
    10741 actgggtcag aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct
    10801 tatcgctgat aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa
    10861 tcactgcagt tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt
    10921 gatgcgaagt tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg
    10981 accagcggtt tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca
    11041 tttttgctgc cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt
    11101 ctcgaacata attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg
    11161 gcatcgtaaa gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat
    11221 ccacatctgt gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg
    11281 caccggtaac gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat
    11341 aatcatcgaa tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt
    11401 cctttgtata gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt
    11461 ctccagcttg ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga
    11521 attcaaatga aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg
    11581 ctttttcgaa tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg
    11641 gtaaagatgc agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca
    11701 cttcaagtgc aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg
    11761 aataattttc ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg
    11821 tcaagccgat ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga
    11881 aaaagttatc ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg
    11941 ctagatggtc ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc
    12001 tggaaagttt catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat
    12061 aatttttttc ttcataagta taactaagat gtaatccaat gattggctgt actccttctt
    12121 ctacacaaat acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta
    12181 atgaagtgta gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt
    12241 gtaaaagaga ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct
    12301 tttttttcgt taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata
    12361 gcaggcgctg aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt
    12421 tctttcgcaa gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa
    12481 atctggtaaa attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc
    12541 tgggctgtcg ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca
    12601 tatgatttta ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt
    12661 ggtgcagttg cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt
    12721 gagatttctc aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa
    12781 gaactttacg aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca
    12841 caataaaaac agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta
    12901 aaacttgtcc aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt
    12961 gaggcgtaat cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc
    13021 ctaaaataaa gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat
    13081 aacagcaaaa tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc
    13141 ctagttttcc tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca
    13201 agccgctgac aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca
    13261 aaatagggct gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga
    13321 tcaaaataga gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt
    13381 tctcgattgg atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca
    13441 acaaagcccc tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc
    13501 ccattgaagg tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc
    13561 atccgctctt ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc
    13621 cagacaaaat cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag
    13681 ccaaagatcc cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc
    13741 cttttcgatc agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca
    13801 cagagattgc tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa
    13861 cataaactgg tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa
    13921 tccacgcaat cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct
    13981 cttttatcat acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa
    14041 tcccgcaaag gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa
    14101 caaatactgt tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata
    14161 atgaataaaa tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt
    14221 actttcacaa ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac
    14281 aatcccataa agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg
    14341 atgtctgcaa aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct
    14401 tgatttggta caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc
    14461 actactactg aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca
    14521 taagttgttg tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca
    14581 aatagtgctt tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct
    14641 tcttttgaga aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa
    14701 gtatcagata aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca
    14761 ctgaataaag attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct
    14821 ttaaatgaat tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc
    14881 ataaaaatac caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt
    14941 tttacaacta aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg
    15001 tcaaagttgg ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg
    15061 ccttcaacga cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca
    15121 tttttgattc cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga
    15181 ctttcgggaa tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg
    15241 attaatccgc aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa
    15301 acgaccgtaa aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca
    15361 tttgcgaaca atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat
    15421 actgcttgga agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa
    15481 ctcatggc
//
LOCUS       DAYYNG010000062        15423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000062 DAYYNG010000000
VERSION     DAYYNG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.062"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..697
                     /locus_tag="ACHBFA_001529"
     CDS             290..697
                     /locus_tag="ACHBFA_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395023.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="ACHBFA_001530"
     CDS             711..1112
                     /locus_tag="ACHBFA_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395024.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="ACHBFA_001531"
     CDS             1114..1485
                     /locus_tag="ACHBFA_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395025.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1521..1826)
                     /locus_tag="ACHBFA_001532"
     CDS             complement(1521..1826)
                     /locus_tag="ACHBFA_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9395026.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2353..2425)
                     /locus_tag="ACHBFA_001533"
     tRNA            complement(2353..2425)
                     /locus_tag="ACHBFA_001533"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2390..2392),aa:Lys,seq:ctt)
     gene            2806..3375
                     /locus_tag="ACHBFA_001534"
     CDS             2806..3375
                     /locus_tag="ACHBFA_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9395027.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3362..4222
                     /locus_tag="ACHBFA_001535"
     CDS             3362..4222
                     /locus_tag="ACHBFA_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9395028.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4412..5116
                     /gene="yycF"
                     /locus_tag="ACHBFA_001536"
     CDS             4412..5116
                     /gene="yycF"
                     /locus_tag="ACHBFA_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9395029.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5121..6956
                     /gene="walK"
                     /locus_tag="ACHBFA_001537"
     CDS             5121..6956
                     /gene="walK"
                     /locus_tag="ACHBFA_001537"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM9395030.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6953..8269
                     /gene="yycH"
                     /locus_tag="ACHBFA_001538"
     CDS             6953..8269
                     /gene="yycH"
                     /locus_tag="ACHBFA_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM9395031.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8270..9139
                     /locus_tag="ACHBFA_001539"
     CDS             8270..9139
                     /locus_tag="ACHBFA_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM9395032.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9194..10003
                     /locus_tag="ACHBFA_001540"
     CDS             9194..10003
                     /locus_tag="ACHBFA_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9395033.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10106..10735)
                     /locus_tag="ACHBFA_001541"
     CDS             complement(10106..10735)
                     /locus_tag="ACHBFA_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9395034.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10905..12197
                     /locus_tag="ACHBFA_001542"
     CDS             10905..12197
                     /locus_tag="ACHBFA_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395035.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12273..12935)
                     /locus_tag="ACHBFA_001543"
     CDS             complement(12273..12935)
                     /locus_tag="ACHBFA_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9395036.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13160..13444
                     /gene="groES"
                     /locus_tag="ACHBFA_001544"
     CDS             13160..13444
                     /gene="groES"
                     /locus_tag="ACHBFA_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9395037.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13495..15120
                     /gene="groL"
                     /locus_tag="ACHBFA_001545"
     CDS             13495..15120
                     /gene="groL"
                     /locus_tag="ACHBFA_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9395038.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         5230 a         2571 c         3215 g         4407 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt
     1561 atatagtcat aacgccgatg atcgctacgt gcgtccggca catcagtcac gatatcaaac
     1621 aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta
     1681 ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
     1741 tcccacacct tataaaaatc tttgacaaat ttttctgatg gatcgtattt aaaccaaagc
     1801 agatgttcct ctagctcgat tgtcatgttt ttcacctcaa cgaaagtata cgaactaatg
     1861 tttgttttgt aaaggataaa aatagggtag caaccggtac ccaaactagt acccatttat
     1921 caaaaacgac gaaatttatg taaatagaaa aataagaata aacattgatg tatcaacatt
     1981 tatatatctt tagaacaaat gaaaagggtg gttctcataa gaaaaacatg ttcgcacttc
     2041 taggcaaacc aggatttgaa gaccttgcta aagaattgaa cgaacgcctg taaaataagg
     2101 atttgtcgct agatttaaaa aatcagtgat agaaacatgt ttgctttaaa atgaaactag
     2161 tgatagtttt agtgatagtg aacttaaaaa aagcactcaa tttgagtgct tttttattgt
     2221 tattaaaaat taaaaaacta aaatacatac ccgaatttca tacccgaaat ataaaaaatg
     2281 tcatcacgta catctttttc gaaactaccg aagtagccaa agatgtactg atgacgtggg
     2341 tttggcaccc aatgggccgt gaggggctcg aacccgcgac ccgctgatta agagtcagct
     2401 gctctaccaa ctgagctaac ggcccgtaca aaaagtactt aataatagta gcactaagaa
     2461 ttttaaaatg caagactaca tttaaagaaa atcagaaaaa tctttacttc cttataggaa
     2521 atcattcata gattattcac atttgctcgc tattatataa gcataccaac aaacaaagtt
     2581 cttgcaagag aacaaaccct aacacttttt tcatttttta ctcctttccc gccgtctccc
     2641 cggcgggtct cctgttctta acgataaaag acaagaaggc ttagccttct tgttttttgt
     2701 tttcatggaa tcccgctaat caaatcacct agttaaagca actagattga atgtttatca
     2761 taagtctgat atcatgttta tgaaatgaaa aaaggagcgg ttatgatgga aaaaatgcgt
     2821 cagcaattaa tggagtgggg gaaggaacta gaaacattcc gtctgcctca ctgggaagaa
     2881 ctccctgact tagaacttta tatggaccaa gtcagaacgt tagttgatcg ttatctttca
     2941 cctgtgatac aaggagaaaa gcatccattg cttacctctt ctatggtaaa caattacgtt
     3001 aaactaggtt tgataccagc accagttaaa aaaagatata acaaagaaca cgtagcattt
     3061 ttacttgcaa tcactacgtt gaaacaagta ttgactattc ctgaaatcaa agaagggata
     3121 ttgttccaag gaaaaacagt aggaattcgt gaagcctata atctattttg cgatgaacaa
     3181 gaagcagctg tgtggatggt cagccaatta gcacaaggta aatctcatcc gcaaaaattc
     3241 gaccaggcaa cccctgtaga atatattgct gtaagagcgg caactttatc gtttgcaatg
     3301 aaacttcttg cggaaaaaac gattgttcta gaaaccgaat atttaaaaga ggagaaaaca
     3361 aatgaaaaac aataaaatcg caattttagt agattcaggt acggatgtgc cagaagaatt
     3421 gataaaaaaa tacgagatgt atatgatccc attgaaaatt atttataaag atcaggtata
     3481 tacagataaa attgacatta cctctgaaga gatctatcaa cgtttgccag aggaaatccc
     3541 tggtacatct ttgccggatg gtgagtcgat cacagaaatc tttgagcaaa tcaaaaaaga
     3601 cggttatgaa aaggtacttg cggtaacaat ttccagtgga ttgagcggga catataatgt
     3661 ggttcgttta attgcacaac agcaagaaga attggaagca tatgtactag atacgaaaaa
     3721 tattggtatt ggtgcaggtt tttctgcgat ccaagcagca aaatggctgg aagaaggagt
     3781 agaatgggat caactgatca gtaatctgaa tgagttagtc gaaagaacaa aagttttctt
     3841 taatgttgcg acattggaat atcttcaaaa aggtggacgg atcggtttag ttgcttctat
     3901 tgtaggaacc gctttaaagc ttaacccaat catctcatgt aatgaagaag ggatttatta
     3961 cacagtagga aaagctaggg gacgtaaaaa aagtttggat aaaacggttg cttacgtcaa
     4021 agaaaaaatc ggtaatgcaa aggtcttcaa cttagctgtt gcccatgggg atgcgaaaga
     4081 agaagcaatt gaaatggaag cgagacttaa aagagagttc ccacaagcaa accaaatcta
     4141 ctttggccag atttctccgg cattagtagt ccacactggt ccgggattat tgggtgtcgg
     4201 tgtacaagtt ttagaaggat aaatcaaaaa aaggaaacac tgaggtgaaa atcagtgttt
     4261 tcctttttta ttgaagttag aacaaaaaat aataaagtat tttgcacttt cattttaatt
     4321 ttctcttttg ttttataggc aaagatgaaa caaaagcaga aatacggtat aatgagaagg
     4381 tatgacaata atatataaga ggtggctctt tatgaaaaaa gttttagttg tcgatgatga
     4441 gaagccgatt tcggatatcg tcaaattcaa tttagccaaa gaaggctatg atgtttatac
     4501 tgcatatgat ggggaagaag ccttggaaaa agttgctgag gtagaacctg atttgatttt
     4561 acttgatttg atgctaccta aaatggacgg cttagaagtt gctcgtgagg tacgcaaaac
     4621 ttatgatatg ccgatcatta tggtaacagc caaagattct gaaattgata aagtcctagg
     4681 cttagagtta ggcgccgatg attacgtgac caagccattt tccaaccgag aattagttgc
     4741 acgtgtcaaa gcaaatcttc gtcgcggtgc cacggctgcg aaggaaccag aagaggcagc
     4801 acctgcggaa ttgacgattg gtgatttgac gatccatcca gaagcttaca tggtgacgaa
     4861 acgaggagaa acaatcgagt taacacatcg tgaattcgaa ctactctttt acttggcgaa
     4921 gcaccttgga caagtgatga caagagaaca tttattacaa acggtttggg gttatgatta
     4981 ttttggagat gtacgaacag tggatgtaac tgtacgtcgt ttgcgtgaaa agatcgaaga
     5041 taatccaagc catccaaatt atttagtgac acgtcgtggt gtcggttact atttgcgtaa
     5101 cccagaacag gagtaattgg atgaaaagaa aagtccgctt ttttcgatca gtcaatttta
     5161 agatcgctat tacctttatt ctgattttat tgatctcgat cgaaattatt ggtgcctact
     5221 ttatacgtgg tttagaacgt tccacgatca atacatttat taaagatatg aatcaaacgg
     5281 tggaatcatt agcaacaaca atcagcccgg aattgaatcg aaaagataat gcagatgatg
     5341 aagaggtaaa tgcgaatatc aaaagattta ttgaaaacag tgcgactagc gatatcatcg
     5401 aaatacgggt cgttgacgaa aaagggatca tccgaggaac aaccgatgtc aatgaacaaa
     5461 gcgctgtggg aaagaaaaat gattatgttg atatcaatga tttcacgact aaacgttatg
     5521 ttgctttaga taacgacgat aaacgggtga atatcaatgt tcaaccgatc ctgtccccga
     5581 ccggcgatgc cgttatcggg gctctttacg tcaaaagtaa tatcgaacaa aaatattcgg
     5641 aaatcaataa cacagcagtc attttcttta ctgcctcact aattgcggca tttatttcaa
     5701 tgatcgtatc tgttttagtt gctcgttcga ttacacagcc gatcggtgaa atgagagaac
     5761 aagccattcg aatcgctaga ggagactaca gtcggaaagt caaagtgcat ggacaagatg
     5821 aacttggaca gctggctgat accttcaatc agttagctga acgtattgaa gaagctcaag
     5881 atacaatgga agctgaacgc aatcgtttgg atagcgtgct atcccatatg acagacggcg
     5941 tcattgcgac agatcgcagg ggtaaagtca ttacgatcaa tgatatggct gtttccttgc
     6001 tagatatcaa aaacgaggaa gcaatcggac aatcgatttt gacactttta gatatcgaag
     6061 aagaatatac gttgcggaaa ttattagaag atccaaacga aatggtactt gaccgttcaa
     6121 ccagtacatt ggaaagtgat cagatgatct tgcgagtaga cttttctatg atccgtcgcg
     6181 aatcaggttt tatcagcgga ttagtagctg tcttgcatga tgtgacagaa caagaaaaaa
     6241 cagaacggga acgtcgtgaa ttcgtctcta atgtttccca tgaacttcgt acaccgttga
     6301 caagtatgcg tagttatatc gaagcattga gcgaaggtgc gtggaaagac gaggaagtag
     6361 caccaaattt tttgaaggta acgctagatg aaacagaccg aatgattcga atgatcaatg
     6421 acctcttaaa tctatctcgc atggatacag gtaataccca gcttcaatta gaatatgtca
     6481 atttcaacga gttagtgaat tttgttcttg accggtttga tatgatggtg ggaaatcagg
     6541 aaaaaaatta taagatccgt cgtgaattta cgcaaagaga tctttgggtc gaagtagata
     6601 cagataaaat catccaagta gtcgataata tcatgaataa cgcgatcaag tattcaccag
     6661 atgggggaac gattacttgt cgcttattag aaacgcataa taacgtggtt ttaagtatca
     6721 ccgatcaagg attggggatt cctaaaaaag atctgaaccg agtgttcgag cggttctatc
     6781 gagtagataa agcacgtgca agagcacaag gtgggacagg acttggatta gctatttcta
     6841 gagaagtgat taaggcgcat cgaggcgcta tttgggcaga gagcaaggaa ggaaaagggt
     6901 cgacttttta tatctcgctg ccatatgaac cttatgagga ggaatggtgg gaatgagaat
     6961 ctctgaaaaa gtcgttcgta taggactcat tttgatggtt gcgttaagca tatacttctc
     7021 ttatgcaatc tggctgagcc cagcaggaaa aagttcaatc aacgtagatg agacaaacac
     7081 tcaaatgatc gagtctcaaa gcaatcgaaa aacttccgaa gtctttcttc cgcttcatgt
     7141 tacatggcac cattcgggaa tgatccaaga aaccaacagt gaaaatctcg tgtaccgatt
     7201 gcaattgatc atcgataaag caagatttgg aaagctttct gaagtagttt caggagacaa
     7261 atcacagttt gaacaggtga aaaatctgaa ctctggtttt gagttggcct ataatgcgcc
     7321 ttttttgcta agagaataca aggaagcatt tgatttagat ttagatgtgt cgaacttggc
     7381 aaaagaaagc gaaaatatgt attttactag aatacaattc gatcaaggaa ataaaaaaat
     7441 ccgatttttg aattattcga accatctcgt atatgaagca aatatttcgt tgaattggac
     7501 agatatcaat aaagaattaa cagcaaaaga cacaaaatgg acagaaatga caacagaacc
     7561 atcgacgatc gacacgcaat atgctacgaa acattcgatc aaactgaaaa agtatagtta
     7621 tatcctgtca cagcaaccct atacactttt tagaaatgct tttttccaga aaccagatag
     7681 cgtgaaaaat aatgatgaaa gcaatgaatt gattttttac ggtgggaatg aaacaatgtc
     7741 tatccattcg gaaacgcaag tagttgattt tcgtggagag ctgccagaga aaaaagaaaa
     7801 tgctagtgta ttttctgaaa gtttccccta tattagcaaa ttaggcagtt ctttagggaa
     7861 cctcagatat tttgaccgca gtgacaatac gatcgattat cggatattcg tggaaggctt
     7921 cccggttttt gggacagaca gcagaggaca gattggtgtg gaagttggcg gtgaaacgaa
     7981 tggttctgtg caagtgaata tccaaaccag tttgaatacg attcaagtgc cgataccttc
     8041 agacgaagaa gtagaacttg caagtacgaa tgaaatcatc cagcagttaa aagctaaggg
     8101 agcagatagc aacaagattg gttcagtgat cgttggctat acatggcaaa atgtcaaaga
     8161 aacgaatcgt gtcgttgatc tcttacctga atggtatgtg aaatataaag ataactggta
     8221 ttcagctaac gaccttctgg aaaaattacc tggattggag gcgaattaat tggatttcaa
     8281 aaaaatcgaa tggatatttt tcgtcgcttt tttagggttg aatgtgtttt tattcagcag
     8341 ttatcatgac gcggtatacc aagaaaacaa tgtcattcgc tctaatcaga ctgaatcaat
     8401 ccaacaacgt ctggctagcg acgatatcac ttacacgggg aagatttcgt ctgagaacaa
     8461 gcaaggctat tatcttagcg cggagcagac caatcttagc acttcattag cgaattatcg
     8521 caaggctggt aaaagcggct tgctggcaag tggtgtgttt ttagacggtg atgttttgac
     8581 gaaatcttta tccagttcag atgctgaaaa gaacgtgatc gatccagata aaattagtag
     8641 ttccttggaa gattttttat ctaacaaaga caatatttta ttcggtaatg attatgtgta
     8701 cacgaaaaac ttttctcatt tagaagaaga accactagaa atccaagcaa gccaagagta
     8761 tgaaggcatc ccattcaatg atgatacagc aaaaatcgtt ctttctttag aaaaaaacga
     8821 taatgattat agcatcacga aatacatgca gacgcattta tctgatatcg agcagctgcg
     8881 cgaaaagacg gaactgtata cggaagagga agcaatcaat acattgtaca tcaataacaa
     8941 aataccaaga ggatcaaaaa ttttatggcg tcagctagct tattcctgta ttctgaaagt
     9001 ccgggaaaaa aatgtgtatg ttcctgtttg gcatgtagcg atcgaaacaa gcgataaagt
     9061 ggtccaaatt gagagtgtca acgctttcag caatacgatc gtgacaaaca atactgtacc
     9121 aaaagtggaa gatcaataaa aaagcgagta taatgaaaaa agtttacaaa gtaagaaggg
     9181 aagtaggagt caaatggctg aagaaaaacc agcttttaaa atcagtatcc tagcaagtgg
     9241 aagttcgggt aattcacttt atatcgaaag tgaaaaaaaa cggcttttgg tcgatgcagg
     9301 acttagcggt aaaaaaatca cttccttgat ggctcaaatt gatcggacac cggaccaatt
     9361 agatggaata cttgtgacgc atgaacatcg agatcatata catggcgtgg gtgtcttggc
     9421 tcgtaagtat catttggatg tttttgccaa tcagcagact tgggaagcga tggaaccact
     9481 gattggaaat gtaccagtgg aacaaaaaca tctatttgag atggggaaag tcagaacatt
     9541 cggcgatatc gatattgaga gtttcggcgt ctcccatgac gcagcggctc cgcaatttta
     9601 tcgtttccat aaagaaaatc ggtcattcgt catgttgacg gacaccggat attgcagtga
     9661 ttatatgcga ggtgtcattg aaaatgccga tggttatttg atggaaagta atcatgatct
     9721 tgagatgtta cgcatgggac cttatccgtg gaatttaaaa cagcgtattt taggagatag
     9781 aggacatctt tccaacgaag acggtgcact tgtcatgact gatgtgatcg gtaatcggac
     9841 caaacgaatc tatcttggcc atcttagcaa ggaaaacaat atgaaagaac ttgctcattt
     9901 aacaatggaa aatgttttga aagaaaaaga ttttggcgtt ggacatgatt ttgaaatcta
     9961 tgatacagat ccagatgaag caactgagct attttcgatt taaacagttc aatcaataaa
    10021 aatataaaaa gcagaagata aaatcaatca gggttggttt tatcttctat ttttattttt
    10081 ttagcatatt aaaaaaataa ccgtattatc gactgactac actgatgtgc tgcttttcat
    10141 gtttctcatc cagtatctca tctactaatg caatcgcgta atctgccatg ctgatcgtac
    10201 tgtcgccagc agcgtttctt tgtaaataat catcacttaa caaataagta cctgtacgtt
    10261 tgccatgtgg atcaaataaa gaagctggac tgatataagt ccaatgtaga tttttagatt
    10321 gtttcaattt taacaatcct tcgtacatat tgaaagctgt tggatagaag ggtgcatttt
    10381 taggtacttg gtcaatcata cgagtcgtct tttctgtatc taagaataac gaagaggcac
    10441 ccccaacgac aattaagcgt gtatcggttc cttcaagcat ctcaatcaaa tgggacaaag
    10501 aagtttgatg catttcttct tttcctttgg gagcgttgaa agcatcaacg acaacatcaa
    10561 aagatgcaag atcagtagtt gtcaactcaa ataaatcctt ttgtataaac ggaacatctg
    10621 atggcaattt ttctggatga cgcacgattg ccgtcacgtc taaatctctg gacaatgctt
    10681 cgtctaaaat aaatgaacct gcatggccag tagctccaat gagtgcaatt ttcataaaaa
    10741 acctcctttt ttattcctgt ccattgtaag cagttgctga atattcgtca aagaatccga
    10801 tttttgaaaa gctcagtgcg cattcctttc atggttcgtg atcattgggt atagttaaat
    10861 taagaaaact gattgataaa aatagaagtt gaaggagata agaaatgaaa gagtggttag
    10921 atcgtgcctt tttaccaatg ataacatctg ttgtgaatct tagaccctta actgctttgc
    10981 gtaatggaat gacttacacg atcccaataa ttattgttgg ctcgctgttt ttcttatttg
    11041 ctcattttcc tgtggattac atcgctcagt ggatagacaa aacaggattt tctatgtatt
    11101 ttacacaagt ataccaggct acatttgatg tgatagcctt ttggatcgtt tttgccattg
    11161 catacgctta tgcaaaagaa gaagacatcg atcgcttacc agtcggcata acagccttgt
    11221 ctgcttatct actgcttatt cagccactaa atactcagat agatactgtc tggacaggtg
    11281 ctgaagggat actaggagca gttacaactg ggctcatcat cggatggggc tatgcacgtt
    11341 tagtaagact tgcgaaaggg tggaagcaaa gagagaagat acctgaaaat gtttggcagt
    11401 cttttacttc attgattcca atcattgtta tagtcacttt tacgcttatc ggttcagcgg
    11461 tttttcaaca gttgttcaga atgactgttg ttcaaggagt atggacatat gtccagcttc
    11521 ccattcaatc gttcatagac acgttgtttg gtgtgctttt attaggtttt tttgttccgt
    11581 ttttatggtt atttggtctt catggatctg cgatggtcaa tgggttggtt agtcccgtct
    11641 tgcaagcgaa ttcattagcg aatgcagaaa tcttagcctc aggacaagag ttgacggtgg
    11701 caaatggcgg acatatcgtt actcagcaat ttctcgatca gttcatgacg gttacgggtg
    11761 ctggattgac gttaggggct gtatttttta tgacggtttt tgcgaaatcg aagaaataca
    11821 gagaattagg aaatcttgct cttttaccag cctttttcaa catcaatgaa tcaattattt
    11881 tttccacacc gatcgtgatg aacccgatga tggctgttcc ttttattttc gcaccggtcg
    11941 tttcaggatt gattacctat tccgctttat attttggatt cgttccttta tttacagcag
    12001 tccaagtccc ttggacaaca ccgccgatcc tctcgggttt cttagtaggc ggagtgcctg
    12061 cagcgatttt acaaggtatc gtgttgtcaa ttagtttttt catttatttc ccatttttga
    12121 aaaaaataga ttctgcaaac tgcaaaaagg aaagacagac aaaaaatgat ggagcagcag
    12181 aaaagatgaa cgactaaaaa acaaaaatac tcctgtcgct ttagattggg aaaatcacca
    12241 atctaggatg acaggagtat ttatagtcat gtttatacta gccctttttg gacagctaat
    12301 tgaacaagaa caactaatgt gttcattcct acgtgtgtga tcatcgatgt ccagatccga
    12361 ccagttgctt tgtactctag gctgaagaag aaacctagga aaaagtagat tagcagatga
    12421 ccatcattat gagcaaacgc aaacagcaaa gagctgatca ctgctccgac ccaaaaattt
    12481 gaatatcgac tgatgatccc cagtatactg cggcggaata cgaactcttc catgatgggt
    12541 ccagcaacag ttgtcgccaa aataaaagcg ggtgcttcca tgatcatctg gatgatattt
    12601 tgtgtattct cggaaggggt tgctttgcca aacaatacag attctaacat gacagcgatg
    12661 ctttgcagaa tcaatgcgac aaagattcca accaatccat aaataacaat ctttggagca
    12721 gaagcttttt ttaccgctga ttcgatcgct aagggttcac gttgcttaaa ataaaaaata
    12781 gctaggacaa cggcgcctag tatatacatg actgcagttg ctgtgatcaa ttcagacgta
    12841 gattttacaa gtcctatcga agctaggatc aagggtgaga taaaaattag accatagcaa
    12901 aaaatgctga ggaagctata tttttttaaa gacatgtaat tcctcctttt aagggataac
    12961 aagcatgcaa ggcaagctta tatcgattga tttcaattat atcataattt caataagcag
    13021 ctgtcagaat aaaatttctt ttctttgatt taaaagcttg caaaaaagag ggagaatgag
    13081 tattattaaa aatgtaggtt agcactcgaa ggttaagagt gctaacagat taaaaaaaga
    13141 gttatcggag ggatttatcg tgttaaaacc attaggtgat cgcgtcatta tcgaagtcgc
    13201 aaaagaagaa gaaaaaactg tcggaggaat cgttttagct tctgctgcta aagagaaacc
    13261 ccaaactggg acagttgttg cagtcggtga aggccgtttg ttagaaaacg gtgaaaaagt
    13321 tcctgccgct gtaaaagcag gcgaccaagt tatgttcgaa aaatacgccg gtacagaagt
    13381 aaaatacgaa ggaaaagaat atctgatcgt cgcaggaaaa gatatcatgg cgattgttga
    13441 gtaaatttgg aagataaaaa aataatcaaa aataatcaat gaggtgaatc aatcatggca
    13501 aaagaattga aatttgcaga agacgcacgc gcagctatgt tacgcggggt agataaatta
    13561 gcagatacag tgaaagtaac attaggacct aaaggccgaa atgtcgtact agaaaaatct
    13621 tacggctctc cattgatcac aaatgatggt gtgacgatcg caaaagaaat cgaattagaa
    13681 gatcattttg aaaacatggg tgcaaaactt gtatcagaag tagcatcaaa aacaaatgat
    13741 attgcaggag acggtacaac gacagcaact gttctgacac aagcaatcgt tcgcgaaggt
    13801 ttaaaaaacg taactgcagg tgctaatcct ctaggcattc gtcgcgggat cgaattggca
    13861 acaaaagcag cagtcgaaga attgcataat atctcaactg ttgttgattc aaaagaagcc
    13921 attgcacaag tagcagctgt ttcttcgggt tctgataaag ttggacattt gattgctgat
    13981 gcaatggaaa aagttggaaa cgacggtgtc atcacaatcg aagaatcaaa agggatcgaa
    14041 acagaactag atgttgtaga aggtatgcag tttgatcgtg gttacttatc acaatatatg
    14101 gtaacagata acgacaaaat ggaagctgtt ctagaaaacc catatatctt gatcacagac
    14161 aaaaaaatct ctaacatcca agatattctg ccattgttag aacaaatctt gcaacaatca
    14221 cgtccattgt tgattattgc tgacgatgta gatggtgaag cattgccaac tcttgtattg
    14281 aacaaaatcc gtggaacatt caacgtagta gctgtaaaag ctcctggttt cggcgatcgt
    14341 cgtaaagcaa tgcttgaaga tatcgctatt ttgacaggtg gtacagtaat tactgacgat
    14401 ctaggtttag aattgaaaga tgtaacaatc gaaaacttag gaaatgcttc taaagttgtt
    14461 gtagataaag acaatacaac gatcgtagaa ggttctggag aaaaagaagc aatcgaagct
    14521 cgtgtgcaat tgatcaaaaa ccaaattgct gaaacaactt ctgattttga ccgcgaaaaa
    14581 ttacaagaac gcttagctaa attagcaggt ggtgtagctg ttgttaaagt tggtgcagct
    14641 actgaaacag aattgaaaga attaaaatta cgcattgaag atgcattgaa tgctacacgt
    14701 gccgcagtag aagaaggaat ggtctcaggt ggtggtacag ctctagtgaa cgttatcagc
    14761 aaagtttctg ctgtagaagc agaaggagac gtggcaacag gtatcaagat cgttgttcgt
    14821 gctttagaag aaccaattcg tcaaatcgct gaaaacgctg gttatgaagg atcagtgatt
    14881 gttgataaat tgaagaacgt ggaactaggc actggtttca atgctgctac tggagaatgg
    14941 gtaaacatgg ttgaagcagg tatcgtagac ccaactaaag taacacgttc tgccttgcaa
    15001 aatgccgctt ccgtttctgc cttgttatta acgacagaag cagtcgttgc agataaacca
    15061 gaaccagcag cgccagcagc tcctgcaatg gatccatcaa tgatgggcgg tatgatgtaa
    15121 gaaaaagcaa cttgcttatc tacaactgat cattagtgaa agacagataa aaagaggctg
    15181 gaacagaagc gtttaactcc aagaaataag aagaaattca cggaaattgc ttttcaaatt
    15241 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgctgtt tatacgtgtt
    15301 tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcttttttg tgtgtaatta
    15361 taatgaaaat agttattttt aatagttata atattttatc tattaaaagt aaatatccat
    15421 tca
//
LOCUS       DAYYNG010000063        15071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000063 DAYYNG010000000
VERSION     DAYYNG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.063"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..831)
                     /locus_tag="ACHBFA_001546"
     CDS             complement(<1..831)
                     /locus_tag="ACHBFA_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395039.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     V"
     gene            complement(860..1177)
                     /locus_tag="ACHBFA_001547"
     CDS             complement(860..1177)
                     /locus_tag="ACHBFA_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9395040.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LDA"
     gene            complement(1202..1522)
                     /locus_tag="ACHBFA_001548"
     CDS             complement(1202..1522)
                     /locus_tag="ACHBFA_001548"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395041.1"
                     /translation="MEKRTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQIESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(1835..3250)
                     /locus_tag="ACHBFA_001549"
     CDS             complement(1835..3250)
                     /locus_tag="ACHBFA_001549"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9395042.1"
                     /translation="MKITIPENFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAKIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYHSGNYGGR
                     IGCILNPEMVYARSSSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCRKQSIYF
                     DPATEDLETVQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEEQYMDEKGIVQDDYRIEF
                     IGQHLRYLIKAAEEGANCEGYMLRAFTDCVSPMNAFKNRYGLVRIELNDERSRILKKS
                     ASWYQSLIDKRELSLPDEAYK"
     gene            complement(3267..4583)
                     /locus_tag="ACHBFA_001550"
     CDS             complement(3267..4583)
                     /locus_tag="ACHBFA_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395043.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLVTGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIVVSILIYLPFVKVANRVENDVI"
     gene            complement(4733..5431)
                     /locus_tag="ACHBFA_001551"
     CDS             complement(4733..5431)
                     /locus_tag="ACHBFA_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM9395044.1"
                     /translation="MEENKTLVEVTTDKILRYLKEKQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQISRMKSLTQKIETSFREGTQDHVQLDIDFHTTIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMATN
                     RRELQKIIGKKETN"
     gene            complement(5708..6697)
                     /locus_tag="ACHBFA_001552"
     CDS             complement(5708..6697)
                     /locus_tag="ACHBFA_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9395045.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRELLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELKIDQILE"
     gene            complement(6804..7607)
                     /gene="msrA"
                     /locus_tag="ACHBFA_001553"
     CDS             complement(6804..7607)
                     /gene="msrA"
                     /locus_tag="ACHBFA_001553"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9395046.1"
                     /translation="MDKEQVFIQTLYNLILNPNTRDWERKVLIQTKNDIRENTSAKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLLTEGFDTQQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGNLSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SDLLTIYWQITDPTDAFGQFQDRGKQYRPVIFYQDERQKKLAEQSKQELVSSGTFRQP
                     IVTKIEPVGTFWPAENYHQQFYKKQPKRYKKIQQARKQFLIYQRIKNKW"
     gene            complement(7842..8585)
                     /gene="truA"
                     /locus_tag="ACHBFA_001554"
     CDS             complement(7842..8585)
                     /gene="truA"
                     /locus_tag="ACHBFA_001554"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9395047.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNSEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHA
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLMGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(8721..9458)
                     /locus_tag="ACHBFA_001555"
     CDS             complement(8721..9458)
                     /locus_tag="ACHBFA_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HFM9395048.1"
                     /translation="MFKYMDKIKQLLDLVEKEEKKSIKEAIDVLTQANLQKRSVYIFG
                     ASHAGILAEEMYYRAGGMMTINAIFGREVMLDRSPITFTSQMERLEGYGTNLAKTVSF
                     KNQDVLILHSVSGRNPIIIDLALTAKAKGVKIISLTNVQYSRSVTSRHSSGKRLFEVS
                     DIVIDNHGDIGDACCELTGIVQKAGPSSTVIGAAILNTIIVEVCEQLIANGIKHPPIF
                     YSANLDGGDLRNAELYEEYKEVIHYEL"
     gene            complement(9459..10709)
                     /locus_tag="ACHBFA_001556"
     CDS             complement(9459..10709)
                     /locus_tag="ACHBFA_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9395049.1"
                     /translation="MNFISEHILKNPPVLLGLIAMLGLFIQRKSFSETVKGALTAAFG
                     MVALTAGVNMLVGTISPINIAVQTQLGVEVTEGLSDVTFTAEYGGTVGLAMFLGLVIH
                     LLIARFTPVKTIFLTGHMLWWFPFVFVAAGVEAGLSGGALIGISGILSACYWSFMPWI
                     MRKYVWDATGDDSFLIGHPTGVLSLVSGFVAKRVGNKEKSTEDIKVPENLSFFREISI
                     TGALVMFLMNTVVGIIAPVLIPEGDNLLMFSIEAGLNFGAGLLIMLYGVRLLINQIIP
                     AFQGIAEKVVPGAKPAFDVPILFNYRPNAVIIGFIVAMITSTILVVLANSFHLFGVLL
                     VPLVITSFFECGGAAVIGEGQGGFRGAVVGTIAASFVMVVLAGFSAIVFSTTIQSWIL
                     IFGGNDLSLWGMIGRGFSGLFGGF"
     gene            complement(10714..10995)
                     /locus_tag="ACHBFA_001557"
     CDS             complement(10714..10995)
                     /locus_tag="ACHBFA_001557"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395050.1"
                     /translation="MKILAVCGFGVGSSMVLKMSLDKVIKELGLKATVENTDLSTAKA
                     TMADVYFTSHELLPELKASVKAPVYPIKKYMDTSEIKAQLEQYLADKGE"
     gene            complement(11008..11439)
                     /locus_tag="ACHBFA_001558"
     CDS             complement(11008..11439)
                     /locus_tag="ACHBFA_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395051.1"
                     /translation="MSTINKLLPKNQIQVIETAENWMKAVEIASNPLLKNKMITHTYL
                     ENMIRSVEENGPYMVLADYFALMHAKPGLGVNEQSMSLLVVKDAVDMKGKPIHIFLVL
                     AAKNHESHLERLKDIMEIFMDNEKYQTILSGNKETIIQLFA"
     gene            complement(11436..13076)
                     /locus_tag="ACHBFA_001559"
     CDS             complement(11436..13076)
                     /locus_tag="ACHBFA_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9395052.1"
                     /translation="MSVIQYQESKLRRVAILNRLSNFEHVSYQQLSEEYYVSRSSIAN
                     DISYIKYVFAKEGLNLSFDRFGTYFEGNEIQVQKVLKRMILNHFDELEIVREIVDRKL
                     LAIVERSFRESIADKQIELPESYFSSIIVSILLMIQRSREGKKISLKGKNQYGKYFLE
                     FNKYPLVCELLHQLEEQRIYQFSQEEVQYLTYVIVGSGLRFFMKNETIPLVFREKIRQ
                     LIQKVSRGIQIDLTQDSRLEEDLLVHLYQLLLRIEAQTTIVNPLIDEIKQNYPSLYGV
                     VWFALNDFRWPYQVSLSEDEVGFVAIHFQAAIERVKRLNRLLFVCPNGMGTSSFVSAK
                     IRRILPDIDSIETASVDKLNRLDLFDVDLIISTVDIPKQIRPVVRISPMVTARDMKRI
                     MNHYIDLVIDNEQINIEGSIAEKVKPLISSNVYFDHFDSKEAAIRFLVDQQQFENEES
                     KQRFLYSIFEREEIQSTYLENGFAIPHGNPNVVKQTSVAVLIADKPFSWGYQKADIVV
                     LLMIREEDVKEVEPVMKMVMQGIEDKNWFISKMLEVRE"
     gene            complement(13296..13931)
                     /locus_tag="ACHBFA_001560"
     CDS             complement(13296..13931)
                     /locus_tag="ACHBFA_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9395053.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EISSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTASIGFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWSFLNKIGFCQRISFFFNYVPIAWLSILHLRQ"
     gene            complement(14083..>15071)
                     /gene="sstT"
                     /locus_tag="ACHBFA_001561"
     CDS             complement(14083..>15071)
                     /gene="sstT"
                     /locus_tag="ACHBFA_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9395054.1"
                     /translation="SALVAVIISSIFTVPITLQETVSEQAPQNLESVLSTMLTNVTQN
                     PVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQISVALSQVVQVIIAFAPIGIL
                     GLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLILFCLK
                     ESALPAFFTRSSAANIPINMMLAEKLKLTKESYSISIPLGATINMGGAAITISVMSLT
                     AVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIVG
                     IGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDRLDGLNER"
BASE COUNT         4559 a         3277 c         2457 g         4778 t
ORIGIN      
        1 gacaaagctg tcgaagaaag gtttgttcac gataaatggt gcgctatgtc cagctgcgat
       61 cgcattgaca tttgcttcga ttgccggcgc attgatcgtt tgcatcaaag gatcgaccat
      121 attcgccccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa gcaataatgc
      181 agatggataa gagttcgcaa gtcccataaa tggttcttgg accactgtat agaacgaacc
      241 tacgatatca tcaaccccaa atgcagcaaa aattgcagca actaaagcaa aggcagagat
      301 agtgatcatt gctggcagta atgaagcaaa tgctttcgca actgctggtg gcacaccatc
      361 aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt ctgttgaaat
      421 aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg ctccgcctaa
      481 agcaaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt
      541 agctaaatga tacgcaagta agaaagcaat caagacagaa agaatggcaa atgtgccgtt
      601 ccaagctgca gccccgaatc ctttccatga ttcaccgcca aaaattgaat tcatcaattc
      661 ttggaatccg ggaatcggta agttattgat cattactgct aatgctccta aaatcattaa
      721 cggcatactc accataaatg aatcccgaat cgcaactaaa tgccgttgtg caccaatttt
      781 tgatgcgaca ggtataaaat gtttctccat aaatgcaaca aatttatcca taaatcttcc
      841 ctcttttctt tactgtgaat tacgcgtcca accgacgata cagatcaatg atctcttttg
      901 ctaaatctgt aaaagcaata gatgtcatca ggtgatcttg actgtgaact gtcaaaagtg
      961 ttacttcaac gtggtttcct tgtgcttctt gcgtcagcat ctctgtttga gagtgatgtg
     1021 cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg aaatctcctt
     1081 ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca aacatgatca
     1141 gacccatgat ggcttctaga tttttttgat cttccatttc ttgtttcctc cgatattttt
     1201 attatttgtt tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat
     1261 agtcagccat attgatcaca tctagaggaa tgcctttagg tgctagcttt tcagcaaatt
     1321 gattttgcat aaaacgtact tgcggaccta agagtaaaac gtctacattt ttagattcta
     1381 tttgattatc tgcatctgag gcagagacag caaagatatc tgcttccatt ccttttgctt
     1441 ctgctgcttt ttgcattttt gtgactagga ggcttgtact cattcccgct gaacatacaa
     1501 gcatgattgt tctcttttcc attttaaaca cgttcctttc tattcttaca cttgtatata
     1561 aagcaagtag tgtgccaact ttaaaagcgc tttaaaaagt tgataatatc acgtttatgg
     1621 tctataccaa gttgcataca cactaaaaaa gaaagcgcac actaaacaca caaattcttt
     1681 tttattgtat ttatttcttt taacgtgcac aaaaaaagtt gtgacaagtc ttttgtcaca
     1741 acttttttga ataagaggtc aaaatgctac tttcacaacc tcctttattc caaataacca
     1801 tcaaatacaa tcaatatctt cgaaaaactt ctagttattt ataggcttca tcaggtaagc
     1861 ttaattctct cttatcaatc agagactgat accaggaagc agactttttc aatattctgc
     1921 tccgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg
     1981 gtgaaacaca gtcagtgaat gcccgtagca tataaccttc gcaatttgca ccttcttctg
     2041 ctgctttgat caaataacgc aaatgctgtc cgataaattc gatacgatag tcatcttgca
     2101 cgattccttt ctcatccatg tactgttctt catgttctct tcccatacca ttttctgtta
     2161 tcagccaagg gatattatga taattttctt ttagatacat ggctatatcg tacatgattt
     2221 ttggatagat ctcccaccct ctagaattgt tcattttctt tccaggtaaa tcaaagactt
     2281 cataatatcg ttctggatgg aaaggtgtct cttcattcca ttgataacga ggagcacgaa
     2341 cgcgtttagg gtaatattga ttgatcccaa ggaaatctac tgtatgatgc tgaaccgttt
     2401 ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt tcgacacaac tctaggaact
     2461 cttctgtgta gtaccctttt accatcggat caaaaaatac tcgattataa aacagatcat
     2521 acatataagc agctttttgg tcttctggag aactggaacg agcatagacc atctcaggat
     2581 ttaaaataca gccaatcctt ccgccataat tgccactatg gtaaatttct acagctttag
     2641 ccgttgctaa tactttgtga taattccaca tcatccattt ttttgtgttt tgttcatgcg
     2701 gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa
     2761 accagttttt taccctgtca ccgtaacgat caaaagcaat ctttgcgtac ttagcataaa
     2821 aatcaaccac ttttcttgaa gaccagccat catatttttc caaaagaaaa gcagggactt
     2881 cgtagtgttc taaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt
     2941 cacttatatg ctgagcatat tcttcatcta ctatacaatt ctcgtaatca gtgaaaaaac
     3001 gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc aaatctacat
     3061 cctgttggta ctgctccata aaattagttg ctacggctgg accataccca ttatgccaaa
     3121 caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt
     3181 tccaaccttc cgtttgccaa gcagaagatg ctgcaccaag aataaaattt tctggaatag
     3241 taattttcat gatttcctcc tgttttttag atcacatcat tttccacacg atttgctact
     3301 tttacgaatg gcagatagat caaaatagaa acaacaatac aaacaagttg cgtaataact
     3361 gcaccgatat ttcctgctgt ggccagataa gcattgacga tcggcggcat cggccaagga
     3421 accattacaa ctgcttttgc cgcaaatcct aaagcggttg caaaataacc aatcgttcct
     3481 gtaacaagcg gcgttaagat aaacgggata gctaaaatag gattcagcat gatcggcata
     3541 ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagagataa cttggtgatt
     3601 tgtttcatat cttctcgctt tcccactagc aatactgcta tcagtagacc tatcgtgacc
     3661 ccagacccgc ccatactcat atacatatcc caaaatggca tagtgataat gtttggtact
     3721 tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttct
     3781 cgaattggct tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaatg
     3841 aacatcaata acaaaatacc tggtagccct tgaacgacac cttctaatgg gcgttgtacg
     3901 acattgtaaa tgatatcata aaggtacatc cctgtaagtt ctttgatgat aaaccccgca
     3961 gccgcgatac ctgatactgt cagaatcgtg ggaaataacg cagaaaaact ggcagaaaca
     4021 ttcgttggca cggtatcagg cattctcagc tgtagttttt tctgtttcct caaccagcaa
     4081 taaagttcaa tggaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta
     4141 tattgtttag ccaatacgtt ctctactata acagtctgat tttcattcac taattcataa
     4201 acagttggat taacagataa ataagacatc actgcgataa taccagggaa tacaccatcc
     4261 actttattca gctttgctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa
     4321 cttagtgtga catagctgat ggcagatgcg ataggtttta attcttctaa aaaacgcaaa
     4381 aaacttatct gtgctaaccc attttccgaa tcaaagacca ttgctgaaaa caacgtgcca
     4441 aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata
     4501 taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata
     4561 aaactcattc cagatcccct cattcctgct tcttctcctt gccgtcatct ctttttgcct
     4621 gttagtcagc gcttacattt cggtgaataa aaactatttg cttgtccgtt ttaccaaata
     4681 taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag
     4741 tttctttctt cccaattatt ttctgtaatt ctctccgatt tgttgccata tggacagtca
     4801 tcgcatcaaa tgccgcaagt acattccgcc tctcgatagc ctgaacgatt tctgtgtgga
     4861 tatcgatcgt ctcgattttc aaatgctgga tatcataatt tttgttaaat aaagcaatcg
     4921 attcattaat gatcggaatg atatgcagta acgctacgtt tccgctagct tctgcaatcg
     4981 tcgtatgaaa atcgatgtct aattgaacat gatcttgtgt accttctcga aaagacgttt
     5041 caatcttttg cgttaagctt ttcattcggc tgatttgatt tttcgttgca tattccgccg
     5101 ctaaccctgc catctttggc tccagtaaat atctcagctc aaacaagtct cgtatcatcc
     5161 ggtcctgatc ttcaatcaaa gaaaaaccga gtggatcatt agcaatccct cgcttatcac
     5221 tgacaaatgt ccccgcccct tgccgaactt caagaatatt cctagaagca agcgctcgga
     5281 cagcttctcg caccgtgctg cgtcccacct ctaaataatc ggacaactca tattcattcg
     5341 gcaatttatc tcctacttgc aactgctttt cctttaaata tctaaggatc ttgtccgtgg
     5401 ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat
     5461 tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat aaaaatcgat
     5521 tcatccgttc atggatatac aaacgaagct atttaccata aaaaaagact aggataaaat
     5581 tcactcctag ccttcgatca tttttatttt gaacgacaaa acgtatgatg ttcaataata
     5641 gatgagcttc attcgattct tgatgacatt ttttgataga tggataaaaa gtttgattac
     5701 atcgtttcta ttctagaatt tggtcgatct tcaattcttc ttctggtgag aaatctaaat
     5761 tatccatcat tcgaacactt tcttgtaact gacttaaccg acttgctcca accagtacac
     5821 tcgtaacaga tttttgtcgc aaattccatg caagggccat ttgcgctaat gattgccctc
     5881 gtgatacggc gattgtctgc agtgctttga ttttttccag agttgatcca acttgttctt
     5941 ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc ttcaggaatc ccatgcagat
     6001 aacgatttgt cagcaaacct tgggcaagcg ggctgaacgt gattgctcct agaccttctt
     6061 cttctaatac tggagataag tcgtcttcta gtagttcacg agaaaacata ttgtagcgca
     6121 tctgatgaat aatgaacggt gcttcttttc tttttaaaat ttcagtcatt tttttggtat
     6181 cttctccatt atagttggat ataccaatat ataatgcttt cccttgtctt acaagttgca
     6241 tcaatgcttc tgctgtttct tccaaaggtg tatcagggtc tggacgatga tgatagaaga
     6301 tgtcgacata atctagcccc attctttgga gactctgatc acaacttgaa atgatagatt
     6361 tacgagagcc ccattctccg tatggacctt cccacatatg ataacctgct ttgctagaaa
     6421 taatcagttc atcacggtag gcttgaaaat cttctctaaa aatacgcccg aagtttttct
     6481 ctgcagctcc agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt
     6541 cgaatgcacc tcttaaaatc tctctttggt tatggatagg atcataatca ccaaaatttt
     6601 gccataatcc gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt
     6661 ccattttttc ataacgtgca ctatctgcaa tatacaagtt ttctcctcct taccattttt
     6721 aaaacgcttt ctttcgttct gcttttagtt tactacattt taaagaaaag gagttagtca
     6781 aaatgatttt ttcttgtatt tttttaccat ttgtttttta ttcgttgata gatcagaaat
     6841 tgtttacgtg cttgctgaat ttttttatag cgtttcggtt gttttttgta aaattgctga
     6901 tgataatttt ctgctggcca aaacgtacca actggttcaa tttttgtgac gattggttgg
     6961 cggaacgttc ctgaactgac tagttcctgt ttcgattgct ccgctaattt tttctgacgt
     7021 tcgtcttgat aaaaaatcac cggacggtac tgcttccctc tatcttgaaa ttgtccaaag
     7081 gcatctgtgg gatcagttat ttgccaatag attgtcaaca aatcgctata acttatctct
     7141 ctcgtatcat aaatgatctc cacagcttcc acatggcctg tgtaaccacc acttacttga
     7201 tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacgga taggttccct
     7261 tttttcgatt caaacggctc gaccatgcac cagaaacatc cccctgcaaa tactgcacgt
     7321 tcttgatagt cagcatcttg catagcatgc ttggaaaagt catatttttg ttgcgtgtca
     7381 aatccttccg taagcaataa ataaaagtcc attacgtcag gcgtcagatt cattcggata
     7441 gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttagcgga tgtattctct
     7501 cttatgtcat tctttgtctg gatcaatact tttcgttccc aatctcgtgt attcggattt
     7561 aaaatcagat tataaagtgt ttgaatgaat acttgttcct tatccataga ctctcctcct
     7621 ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataagc
     7681 gattgaatcg tatctattca aagaagataa gcggcttcag atgatataag atgatcttta
     7741 tcttaaaaag tatactctat ggtttggttt aactaaaaaa cgacctctga aaaaagggat
     7801 gttctccagt tctcagaagt cgagtaaaga tgattctttt tttatgaacg ataataaact
     7861 tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgctgccg cactttgctt
     7921 tcgaatattt catcgattgt ttctatcggc atggcacctg taccgatttc tagtaatgtt
     7981 cccatgatga ttcgcaccat tttatataga aatccgtctc cgacaaaagt aaaatgaagc
     8041 atttctccct tacgttcgat agataattca tcaatcgtac gtaccgtaga tttttttgat
     8101 tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca tcaatttttc gcaagctgca
     8161 ttcagcttat ccatatccaa ctcttgagga aaataaaagc tgtgatttcg ttcaaatgct
     8221 gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc attatatctt
     8281 gcatggaagc gctcaggcat ctcttcgatt cttttgatga caatatctcg cggaagatag
     8341 cgattcataa agtcgatcat ttctgccagt ccaatttctg agtttgtttt gaaatttgcc
     8401 acttgtcctc gcgcatgcgt gccggcgtct gttcttcctg atccaataat ttctatctct
     8461 tcatttgtca tctgatgcaa cactttttcg attttccctt ggatggtttt atcgtcatct
     8521 ccaagtcttt gccagcctga atatcttttt ccgtcatatt cgatcgtcaa cttgatgttt
     8581 ctcatatttt tgtggctcct ctatattcca tttggtcttc tctctacctt tttaagattg
     8641 tagcacaaat taaagctact tgcttctctg tgagagaaac caagtagctt taaataattg
     8701 tttatgaaca attgtattat ttataattca taatgaatca cttctttata ttcctcatat
     8761 aattctgcgt ttcgcagatc gcccccatct aaattcgccg aataaaatat aggaggatgt
     8821 tttatcccat ttgcaataag ctgttcgcaa acttccacga tgatcgtatt gaggatggct
     8881 gcaccgatta cagtagagga aggaccggct ttttgtacga tacctgtcag ctcacagcaa
     8941 gcatctccta tatccccatg gttatcgata actatatcag aaacctcaaa caatcgtttc
     9001 cctgatgaat gacgactagt gaccgatcgt gaatattgga cattcgtcaa actaatgatt
     9061 tttacgccct tcgcttttgc agttaacgct aaatcaataa tgattgggtt tcttcccgat
     9121 accgagtgaa ggatcaatac atcttgattt ttaaatgaaa ctgtttttgc taaattcgtt
     9181 ccatatcctt ctaaacgttc catttgactg gtaaaggtga taggggaacg atctagcatc
     9241 acttctcgtc caaagatagc attgatggtc atcatcccac ctgcccgata atacatctct
     9301 tccgctaaga taccagcatg actagcacca aaaatataga ccgaacgttt ttgcagattc
     9361 gcttgggtca atacatcgat tgcttctttg attgactttt tttcttcctt ttcaaccaaa
     9421 tctaataact gtttgatttt atccatatat ttgaacatct agaaccctcc aaacaaaccg
     9481 ctaaatcctc taccgatcat tccccataac gacaaatcgt ttccgccgaa aatcaatatc
     9541 cagctttgga tcgtcgtact aaaaacaata gctgaaaaac cagctaatac gaccatgaca
     9601 aatgaagctg cgattgttcc gacaaccgct ccacgaaagc caccttgacc ttcaccgatg
     9661 actgctgcac caccacattc aaagaaactt gtgattacta atggcacaag caacacgccg
     9721 aatagatgga atgaattcgc caaaacaact aaaatcgtag aagtgatcat tgccacgata
     9781 aaaccgataa tcactgcatt tggtcgataa ttgaataaaa taggcacatc aaatgctggt
     9841 ttcgctcccg gtacgacttt ttctgcaatc ccttgaaacg ctggaatgat ttgattgatc
     9901 agtagtcgga caccatatag cataatgagt agtcctgcac caaaattcaa ccctgcttca
     9961 atcgaaaaca tcaggagatt atccccttct ggtatcaaaa caggtgcgat gatcccaact
    10021 acggtattca tcaagaacat gactagtgca cctgtaattg agatttctcg gaaaaatgaa
    10081 agattctcag gtactttgat atcttctgtc gatttttctt tatttcctac tcgttttgct
    10141 acaaatcctg aaactaaaga caaaacaccc gttggatgac caatcaggaa agaatcatct
    10201 cccgtagcat cccagacata tttgcgcatg atccacggca taaaagacca ataacaagcc
    10261 gataaaatcc cgctgatccc aatcaatgct ccccctgaca gtcctgcttc cacaccagct
    10321 gctacaaata cgaaaggaaa ccaccaaagc atatgacctg ttaaaaagat cgtcttcaca
    10381 ggtgtaaacc gagcaatcaa taaatggata actaatccta aaaacatagc taatccgacc
    10441 gttccgccat attctgcagt aaacgtcaca tcagatagtc cttctgtcac ttctacaccc
    10501 aactgtgttt ggacagctat attgattgga gaaatcgttc cgaccagcat attgactccg
    10561 gcggtcaaag caaccatacc aaatgcagcg gtcaaagctc ccttaaccgt ttctgaaaat
    10621 gattttcgct ggatgaacag cccgagcatc gcaatcaaac ctaacaagac gggcggattc
    10681 ttcaagatgt gttcactaat gaaattcatt cttttattcc cccttatcag ctagatattg
    10741 ttccaattgc gcctttattt ctgaagtatc catatacttt ttaataggat aaacaggcgc
    10801 ttttacagaa gcttttagtt ctggaagtaa ttcatgactt gtaaaataaa catctgccat
    10861 cgttgctttg gcagtggata aatctgtatt ttccactgtc gcctttaaac ccagctcctt
    10921 aattacttta tcaagagaca ttttcaaaac catagaactc cctacaccaa atccacatac
    10981 tgctaaaatt ttcatttttc ttcctcctta tgcaaataac tgaatgattg tttctttatt
    11041 tcctgataaa atcgtttgat atttttcatt gtccataaat atttccatga tatcttttaa
    11101 tctctccaga tggctttcgt ggtttttggc agctaaaacc aaaaaaatat ggattggttt
    11161 ccctttcata tctacagcat cttttacgac taaaaggctc atactttgct cgtttactcc
    11221 aagacctggt tttgcgtgca tcagcgcaaa ataatcagcc aataccatat atggtccatt
    11281 ttcctctaca cttctgatca tgttttctaa atacgtatgc gttatcattt tgttttttaa
    11341 taatggattg ctagcgattt cgactgcttt catccaattt tcagccgttt ctattacttg
    11401 gatttgattt ttcggcagca atttattgat agtactcatt ctctgacctc caacattttt
    11461 gaaataaacc aatttttatc ctctattcct tgcatgacca ttttcatcac aggttcgact
    11521 tctttcacat cttcttctcg gatcattagc agaacaacga tatctgcctt ttgatatccc
    11581 catgaaaatg gcttgtctgc tatcaataca gcaaccgaag tttgtttaac gacattggga
    11641 ttgccatgag gaatagcaaa accattctcc aagtatgttg attggatttc ctcacgctca
    11701 aaaatagagt agagaaatcg ctgtttcgat tcttcattct caaactgttg ctgatcaacc
    11761 aaaaaacgga tagcagcttc ttttgaatca aagtgatcaa aatagacatt agaggaaata
    11821 agaggtttca ctttttcagc tatcgagcct tcgatgttta tttgttcgtt atctatgacc
    11881 agatcgatat aatgattcat gatacgtttc atatctctag cagtaaccat tggcgaaatt
    11941 cttactactg gtctaatttg tttaggtata tcgacagtgg agataatcaa atctacgtca
    12001 aacaaatcta gtctgttcaa tttatcgaca gatgctgttt cgatactatc gatatcaggt
    12061 aagattcttc tgatttttgc agaaacaaat gaactggttc ccattccatt gggacatacg
    12121 aataagagtc tatttaatcg tttgacacgc tcgattgctg cttggaaatg gatagctaca
    12181 aaacctactt catcttcgga caagcttact tgataaggcc agcgaaaatc atttaacgca
    12241 aaccagacaa caccatacaa agaaggataa ttttgtttta tctcatctat caaaggattt
    12301 acgatggtcg tttgagcctc aatacgtaaa agcagctgat acagatggac aagcagatct
    12361 tcttctaaac gactatcttg ggtaagatct atctgaatgc ctctactcac tttttggatc
    12421 aattgccgga ttttttctcg aaatactaaa gggatcgttt catttttcat gaaaaagcgt
    12481 aaaccacttc ccacaataac ataagtcaaa tactgtactt cttcttgcga aaattggtaa
    12541 atacgctgtt cctctaactg atgcagcaat tcacaaacga ggggatattt gttgaattct
    12601 aaaaaatact ttccatattg atttttacct tttaaactga ttttcttccc ttctcttgaa
    12661 cgctgaatca tcaataagat tgaaacgatg atacttgaaa agtagctttc tggtagctca
    12721 atttgcttgt ctgcaatgct ctcgcgaaaa gatcgttcga ctattgctag aagctttcga
    12781 tccactattt ctcttacgat ctccaattca tcaaaatgat tcaggatcat acgcttcagt
    12841 accttctgaa cctgtatttc atttccttca aaatatgtcc cgaaacgatc aaaactgaga
    12901 ttcaaccctt ctttcgcaaa tacatatttg atgtaagaaa tatcatttgc gatactgctt
    12961 ctggaaacat aatattcttc agaaagttgc tggtaactta catgctcgaa attggacaat
    13021 ctgttcagga tcgctactct tcgtagtttg ctttcttgat attgtattac actcatttcc
    13081 atcaccttct atattcacta taattttttc ttataaggag aactagacca aataaaagat
    13141 taaaataacc tgagaaatca tacactgctc tttctcagtt aatatacgct aaaacagaag
    13201 ctatacaaga aaattatgga actatatttc agaaaaacaa aaaaacagcc aacggacgaa
    13261 atgtccgctg actgttcaat ttgatggctc aagttttact gtctgagatg caagatactc
    13321 aaccaggcaa tgggcacata attaaagaaa aaactgattc tttggcaaaa tcctatttta
    13381 ttcaggaatg accaagaatt tatagaagga attcttgcca gaccataaat cgccgcaaaa
    13441 agcaaactga tcacagacaa tccaaggtat aagtggctta cgtccatttg cccatttttt
    13501 cgatagatca agaaaagaag aactggaaac aataaaaaac cactataacc taaacctgat
    13561 ccgatattat ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac
    13621 aaacctgtaa agatacaatc acctatacca taaaaaccga tagatgctgt aaccagggta
    13681 gctaacacac gagatgtact tacaaatgca gcatacaatg caggcaatga caggatgaaa
    13741 aaaatccctg aaaccactga ccaaataaga aatgcttttc ttacaggact ggaaatctct
    13801 ccaaatacac taatcacact cttcatttga tctaattcag gataatacaa tcctaatacg
    13861 tacggagtca aaaaatcact caacacgcct aataataaaa agaagaaacc aaatttacgt
    13921 aaaaaatcca tgatgcgcct ccaataacaa ttacctatgc ttttaattgt tattataaac
    13981 gactttatct cgatttaaca ggatcctcta ttttacacga acatctacat aactcattaa
    14041 aaatttgaat cgtcttaagg ttcatcccta aatatctaat attcaacgtt catttaaccc
    14101 atctaatcga tctttcaatt tgattttttc tccacttctt ttacgcgcgc ctaattctcc
    14161 tattgcagtg aacaacagat cacttgagga atttaaggca gtttccattg aatcttggat
    14221 aacaccgata ataaatccga taccgacgat ttgcatcgca acatcattag agataccaaa
    14281 caaactacat gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagccccaca
    14341 agcagctaaa gcagacaaaa tacataaaag gactttcaaa acaaatggtg cttcaatccc
    14401 taaggaatgt acagccgtca atgacatcac ggaaatggta atcgcagcac cgcccatatt
    14461 gatcgtagca cctaatggga tcgatatgga ataagactct ttcgtcaatt tcaatttttc
    14521 agccagcatc atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag
    14581 cgcactttct ttcaaacaaa aaagaatcaa tggatagggg ttttctttaa taaacaaaaa
    14641 cgtgaggaac gggtacacaa ctaatgcgac aaacagcatc gtgccaatca acacaagtaa
    14701 aagctgcaga tactctgcca atccagcgat tccagtcgtg gcaatcgatt gataaaccaa
    14761 tcctaaaatc cctatcggag cgaatgcaat aatcacttgt acgacttgtg ataatgctac
    14821 agatatttga tccaccaatt cttttgtcga ttctgaacgt gctcgaaaag ccagacctaa
    14881 caacacagcc cagaataaga caccaagata attcgcatca atgagcgatt gaacaggatt
    14941 ttgtgtgaca tttgtcaaca tcgtagataa aacactttcc aaattttgcg gtgcttgttc
    15001 agatactgtt tcttgtaaag taataggaac agtaaaaatt gaacttataa tcactgctac
    15061 aagtgccgaa a
//
LOCUS       DAYYNG010000064        14877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000064 DAYYNG010000000
VERSION     DAYYNG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.064"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHBFA_001562"
     CDS             complement(<1..430)
                     /locus_tag="ACHBFA_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9395055.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            complement(486..1040)
                     /locus_tag="ACHBFA_001563"
                     /pseudo
     CDS             complement(486..1040)
                     /locus_tag="ACHBFA_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(1056..1535)
                     /locus_tag="ACHBFA_001564"
     CDS             complement(1056..1535)
                     /locus_tag="ACHBFA_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM9395056.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1541..2565)
                     /locus_tag="ACHBFA_001565"
                     /pseudo
     CDS             complement(1541..2565)
                     /locus_tag="ACHBFA_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2582..3688)
                     /locus_tag="ACHBFA_001566"
     CDS             complement(2582..3688)
                     /locus_tag="ACHBFA_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9395057.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3918..6515)
                     /gene="adhE"
                     /locus_tag="ACHBFA_001567"
                     /gene_synonym="adhC"
     CDS             complement(3918..6515)
                     /gene="adhE"
                     /locus_tag="ACHBFA_001567"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9395058.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6820..7200)
                     /gene="yajC"
                     /locus_tag="ACHBFA_001568"
     CDS             complement(6820..7200)
                     /gene="yajC"
                     /locus_tag="ACHBFA_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9395059.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(7297..8442)
                     /gene="tgt"
                     /locus_tag="ACHBFA_001569"
     CDS             complement(7297..8442)
                     /gene="tgt"
                     /locus_tag="ACHBFA_001569"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9395060.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8660..9358
                     /locus_tag="ACHBFA_001570"
     CDS             8660..9358
                     /locus_tag="ACHBFA_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9395061.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9526..12165)
                     /locus_tag="ACHBFA_001571"
     CDS             complement(9526..12165)
                     /locus_tag="ACHBFA_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9395062.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12784..13815)
                     /gene="queA"
                     /locus_tag="ACHBFA_001572"
     CDS             complement(12784..13815)
                     /gene="queA"
                     /locus_tag="ACHBFA_001572"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9395063.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            14008..>14877
                     /locus_tag="ACHBFA_001573"
     CDS             14008..>14877
                     /locus_tag="ACHBFA_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395064.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSN"
BASE COUNT         4197 a         3245 c         2665 g         4770 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcaaaagcgc ctacgagata acctcccata aacaaagcag agatcgaagc accaccagca
      541 acaagtgaat acatgataaa agcagtactt ggaggaatga ccatacctgt cggagctgac
      601 gcgatattga ccgctgtcgc aaacttccgg tcatatcctt cttccacttg ctgagggatc
      661 aacactccac caatcgcggt agaagcggca atggccgagc tagagatgga accaaataaa
      721 gcattcccta acacattcgt atgagccaaa gcccccggca cgcgcccgac gaacaacatc
      781 gcaaaatcca ctagtttctt agctatccct ccattattca tgataatgcc cgagagaacg
      841 aaaaacggaa tagcgatcag cgaaaaacta ttgatacttc ccaccatttt ttgagctgaa
      901 ctaaaagcag aaacatcaaa cggtataact aacaataaag tcatcatcga tgagaatgca
      961 atacttatag caatcggcat ccctaataca agcaacacaa caaatacaaa aaataacact
     1021 aatccggctt ctattgccat tttattcctc catttttata ttttttcttg aattgctggc
     1081 ttgttgctta atgtatcgat tagattgatc aggctataga taatgatgag tacccccgat
     1141 acaggtactg acagataaac atatcccata ggaatactta aagaaggcga gatctgcccc
     1201 attgtcaatg tgacagcatt ccatccgcca tacaccatga tcatcgcagc aaataccaga
     1261 aataaacttt gaacaacaac ttctaaaatt ttactttttt tgccagttaa tttttcactt
     1321 aaaaccgtta cggctaaacg tgctttttgt cccacaacat acgcggaaga caacatggca
     1381 aaccagacaa gaccgaatcg aacaagctct tccgtgaccg tatttggatt tcctaaaacc
     1441 gttctggaaa atacttgcca taaaacaaca gcgaccatcc caacaagcaa aacagctccc
     1501 aggatctcta ataccttgtt taaaatcatt ttcattacag ctacgctcct tgctcttgga
     1561 tcttttgata aaggtctttg taggtaggat cgtttttgaa tatatcatga agtggggcaa
     1621 ctgcctctcg aaacggtgta ttatctacat cattgaattg gactccatgt tcttcttgtg
     1681 ctaaagcttt ttcttcttca attgcttttg caaaagcttc aatttcaaaa accgttgatt
     1741 ctttcgctgc ttcttcaatg gctttttcct gttcatcagt tagctgttct ttgatggcat
     1801 cattcatgat gatgatatct ggcacacgtg tatgctcatc ataggaatag tatttagcta
     1861 ccccgccatg tccagctgta tacaagacaa attcattgtt ttctgatccg ttgatcaaat
     1921 tggattgaag agacgtgtac acttcatcac ttcccatagg tacgggcgaa ccgcctaaaa
     1981 gctctaccat tttgatagcg gtttcgcttt gcatcacacg gatcttcttt cccttcaaat
     2041 cttccggcgt atggatcgga ccgtctacca tataaaagct gcgctgacca gaatcataat
     2101 aggtcaatcc ggtaaatcct aaatttttgg tcgattggta gattggctcc attatttctt
     2161 cattatccat caccttaaag aaatgttctt catcattgaa tagataagga acggaaaaaa
     2221 tcgaataatc tttagagaaa ctttccaacg ctgaggcact tacttttgtg aaatctactg
     2281 ctcctgactg tgtcaattcg atcagttcac gttctccacc cagttgtcca tctgggaaat
     2341 actggatctg tacggaacca tctgtttttt cttttactaa acgcccaaat tcattcattg
     2401 catcaattac tggctgttgc ttgcataagc aaagcgcaag gtaactatct catttacgtc
     2461 tgcatctgcg acagttgcca cactagaatt atttccgcag ccgcttagac ctagcagacc
     2521 taggctaagc gaacttgctg caaacagcag tgatgttttt ttcatatggg tttcctttct
     2581 tttaacaata agagacgagc aattctgtca aacaaagcac cgttaacgac tgtccataag
     2641 gcattgctgt gattccgata ttccgataaa aatctaaact gtcccccatt cctgtcccga
     2701 ttgaaacgtt ttgaacttct cctttttcat cgatttgttc aagcaatcct tggatagcac
     2761 gataagcgac ttctttatat tcttgtggta aataacgttt atggaccgct ttcaaaatac
     2821 cgtaagcaaa tcctgcagtt gcagaagatt ctacgtaaga agaaggatca tccaacaacg
     2881 tatgccataa accgctttca gactgataag tttttaatgc acgcacttgt gcttcaagtg
     2941 tttcgatcaa taatttgcgc aatgcatctt ctttcgataa ttctaagatt tcaatgatct
     3001 ctggaatagc aatcgtcaac cagcagtttc cacgagccca aaatgcttcg gcatagttat
     3061 gatttccttc aaacgtccag ccgtgatacc acaatcctgt tcgtttatcc atcaagtatt
     3121 tgatatggat cataaactgg taacgagctt cttctaaata ttctgggcga ttcaacagtt
     3181 tcccgatttt tgccaaagga agcactgtca tcatcaatgt atcgtcccac aactgattct
     3241 tattttccgg tccatatgtg gcatgctgta acccgtcttc atttgtccga ggcatgtcat
     3301 tcatgaccca ttctgcccat tgttctaaat aaggcaaata gcgtgaatct ttcgtgtctt
     3361 catacaagta agccatagtc aatagcggtg ccattgtatt gacgtttttg ggcggagcgc
     3421 cttcttccat acgatcctca aaccattcat tgaccacttg ataagcgcga ggattttttg
     3481 tcatctggta atttttataa ataccatata gaccgacacc ttgtggccaa ttccacactg
     3541 tccagctttt gtcatctact ttcagtccat caaagtccaa caaaaattca ccattatcgt
     3601 ctttgatcgt tgtcaggttt tcaacgagca ggtcaatttt ttctttcact aattctttag
     3661 gtacaaaaat ctcttcttta ataatcatct ttacgccacc ttttttaaac gctttcattt
     3721 tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg aacaaaaact
     3781 gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt ggaaattaaa
     3841 aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc ttatcttttt
     3901 tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt caattcagag
     3961 atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa ttcagccata
     4021 cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag actcatgttg
     4081 atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac taattcctct
     4141 gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt atctgcacgg
     4201 aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt atagcggata
     4261 atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc cccgccgatt
     4321 ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat accagccatt
     4381 gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac tgatttttct
     4441 aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata atctgaagcc
     4501 atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt atctgctgtg
     4561 acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc tggcgtcaat
     4621 gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac cgcaaatggc
     4681 gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt tttttctggt
     4741 ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc accaaagaaa
     4801 gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat tgcagaaccg
     4861 cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt tcctgcgtat
     4921 acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt cacatcattt
     4981 ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac cataccaggg
     5041 tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa tagagagttc
     5101 ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct ccgtttcgct
     5161 acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga gttcttacca
     5221 taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc accgatgccg
     5281 ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag accgaatttg
     5341 acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc tccaagattc
     5401 aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat catggcaaga
     5461 actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc caattcagca
     5521 atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat ttccatcgca
     5581 gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac tgcatcttct
     5641 aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa ttctgctttt
     5701 actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca aatcattcca
     5761 ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt tgctgttttt
     5821 tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc tgtagagtaa
     5881 gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc tggatggttc
     5941 attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca attttcagga
     6001 gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga agatttttgg
     6061 gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat cattgatttg
     6121 aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac accgactggt
     6181 tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac tgttttatca
     6241 tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc tttatcttcg
     6301 taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg ttgatccaaa
     6361 gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa gctttccatt
     6421 tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg tacgtcaatt
     6481 gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat gggtcatggt
     6541 tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg taaacacttt
     6601 tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac aggtgtttgt
     6661 atagaaaaat atataaagtt atttattatt atttatatga cacacccgat tattcaatat
     6721 tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca
     6781 tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc
     6841 tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc
     6901 tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt
     6961 gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt
     7021 gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc
     7081 tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga
     7141 cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat
     7201 ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa
     7261 cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt
     7321 attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat
     7381 tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa
     7441 acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt
     7501 tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc
     7561 atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt
     7621 aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc
     7681 agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc
     7741 taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc
     7801 agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc
     7861 tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg
     7921 ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa
     7981 tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat
     8041 tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc
     8101 ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt
     8161 taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc
     8221 ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc
     8281 tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat
     8341 tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt
     8401 ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta
     8461 tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc
     8521 ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa
     8581 aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa
     8641 agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag
     8701 tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat
     8761 tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg
     8821 atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg
     8881 aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga
     8941 tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa
     9001 aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt
     9061 atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta
     9121 ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat
     9181 cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc
     9241 atttatcggc tggcatatct tagcattagc aactgctggt attggttatc tatggctcag
     9301 cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa
     9361 gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa
     9421 aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt
     9481 tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc
     9541 tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa
     9601 aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt
     9661 aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa
     9721 agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac
     9781 atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact
     9841 tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac caaacaaggt
     9901 tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat
     9961 cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc
    10021 taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt
    10081 aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt
    10141 acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact
    10201 gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg
    10261 cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg
    10321 tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt
    10381 gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg
    10441 cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg
    10501 atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt
    10561 gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg
    10621 acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc
    10681 agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag
    10741 tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa
    10801 gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg
    10861 gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata
    10921 tttttcttct aattctgcct ttgtatgata attctcttca acagccctta cgatggctac
    10981 ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt
    11041 acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt
    11101 cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt
    11161 tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg
    11221 tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac
    11281 aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat
    11341 ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa
    11401 agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg
    11461 catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc
    11521 agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag
    11581 ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc
    11641 actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc
    11701 atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc
    11761 tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc
    11821 ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt
    11881 gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa
    11941 tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa
    12001 gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc
    12061 ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc
    12121 tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta
    12181 aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca
    12241 aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc
    12301 aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac
    12361 agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac
    12421 ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca
    12481 cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga
    12541 aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt
    12601 gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt
    12661 tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt
    12721 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
    12781 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
    12841 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
    12901 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
    12961 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
    13021 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
    13081 tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg
    13141 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
    13201 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
    13261 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
    13321 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
    13381 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
    13441 ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
    13501 aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt
    13561 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
    13621 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
    13681 atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg
    13741 atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc
    13801 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
    13861 tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
    13921 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
    13981 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
    14041 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
    14101 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
    14161 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
    14221 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
    14281 acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg
    14341 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
    14401 tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca
    14461 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt
    14521 gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt
    14581 gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat catgagtgaa
    14641 ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc aaacgatttc
    14701 gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc gacaagagtc
    14761 ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt acaagaagca
    14821 atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa ttcgaat
//
LOCUS       DAYYNG010000065        14691 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000065 DAYYNG010000000
VERSION     DAYYNG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.065"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..720)
                     /locus_tag="ACHBFA_001574"
     CDS             complement(154..720)
                     /locus_tag="ACHBFA_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9395065.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(720..1676)
                     /locus_tag="ACHBFA_001575"
     CDS             complement(720..1676)
                     /locus_tag="ACHBFA_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM9395066.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1676..2332)
                     /locus_tag="ACHBFA_001576"
     CDS             complement(1676..2332)
                     /locus_tag="ACHBFA_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9395067.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2702..4720
                     /locus_tag="ACHBFA_001577"
     CDS             2702..4720
                     /locus_tag="ACHBFA_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9395068.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4790..4996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5147..7561
                     /gene="leuS"
                     /locus_tag="ACHBFA_001578"
     CDS             5147..7561
                     /gene="leuS"
                     /locus_tag="ACHBFA_001578"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9395069.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7909..9462
                     /locus_tag="ACHBFA_001579"
     CDS             7909..9462
                     /locus_tag="ACHBFA_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM9395070.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9843..11627)
                     /locus_tag="ACHBFA_001580"
     CDS             complement(9843..11627)
                     /locus_tag="ACHBFA_001580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395071.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11642..13363)
                     /locus_tag="ACHBFA_001581"
     CDS             complement(11642..13363)
                     /locus_tag="ACHBFA_001581"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395072.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13463..14224
                     /locus_tag="ACHBFA_001582"
     CDS             13463..14224
                     /locus_tag="ACHBFA_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9395073.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4770 a         2712 c         2923 g         4286 t
ORIGIN      
        1 aaacagcttc tcatgttttt gatttggttg gacttattac cgcagatata agcttttgag
       61 caccgtgaat aaagaggtta tgaaatcaac gctctaacct gagtattaga gaaaaaaacg
      121 caaaacatat catttatgtt ttgcgttttt tatttatctt tttttctcaa tacaatataa
      181 aataggcggc tggttctttt ggttgataaa ccgataattc agcacattat atgtttcctg
      241 tggcagtttg gaacaaaaag aatcaaccat atccagttct ttttctccac cttcatgacc
      301 atagtagacg acaaggatca atcgtccgcg aggcacaagt cgtttcaata tttcttccat
      361 agctgtgcgt gtagtctctg gcatagtgat gattgcttta tcactttttg ggagataccc
      421 tagattgaaa atccctgctt tgagattttg ttgttcagct atcacttctc ctaacgtttc
      481 gtggccttgt agaaacaatg agacacgttc ttctaactga cgctgttcta gcctttcacg
      541 agtagcatcg attgcttgtt gctggatatc aaaactatag acatgaccag tttttccgat
      601 gtgttctgct agaaaaaccg tatcgtggcc attgcccatc gttgcatcta cgacgtgatc
      661 ccctggttgc aagatttctt gcagtaatgt atggctgaaa tgcaaagcag tttgcaacat
      721 cagacgctca cctttttccg tacatcatat tttccttgga agctatctct gcgaagaagt
      781 tcttcatcaa ttgcattcaa gacttcccat tttttcaagc tccacattgg tccaatcaag
      841 gaatcccacg gcgcatcccc tgtcaaacga tggatgatga tctcttttgg tatcatttcc
      901 agctggtcgc aaatgacatt gacatagtca ggacggctca ttaattgaag gcgaccttca
      961 tgatagtccc gcaacattct cgttttgcgc atcagatgca tcaaatggag cttgattcct
     1021 tggatatctg aatctaaaat ggtgcgtctg acattttctt tcatcatttc taaactttcg
     1081 cctggtaatc cgttgatcaa atgcgtacaa acacggatat tatgtttgcg taatttagcc
     1141 acgccatcta aataagtttg ataatcatgt gctcggttga tcagttttga agtttcttca
     1201 aaagttgttt gcaatccaag ttctacccat aaatacatcc gctcgttcag ttcagcaagg
     1261 taatcaacta cttcatctgg cagacaatcc ggtcgtgtac cgactgataa tcctacgaca
     1321 cctggtaaat tgactacttg ctcaaatcgt tctttgatta tttcaactgg cgcatgggtg
     1381 tttgtaaaat tttggaaata aacgatgtat tgttggacat gaggccattt ttgatgcatc
     1441 atgtcgattt ctttctggaa ttggattgga agagggtctt caggtgcaac gatcatgtct
     1501 cccgaaccag atacactgca aaaagtacaa ccgccatggg caacagtccc atctcgattc
     1561 ggacagtcaa atcctccatc gatcggcacc ttgaaaattt tcccgccaaa ctcatttcgt
     1621 aaagcgtaat tccaagaatg atatctttta tttccttctg aataaggaaa agtcattaaa
     1681 agtccttctt tctgtaagag ttatctattt gttttctttt ttctctatac actggataag
     1741 tcaggcacag ccaagaaaga ctgagtaagt agccgccgat gatatcagta ggaaaatgaa
     1801 cgcccagata aatgcggctt atcccgacac ttactataat aaacatcatc agcaattgaa
     1861 gcaatgtgcg taaggttctt ttttgaacaa aagctggaag cagaaatagt aaagaaccgt
     1921 aaagaatcat actgcctgct gcatgtccac tagggaaact gtaacttgtt tcaactacta
     1981 agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt gatcaccggg ttgatgattc
     2041 cagcaattac aataaatcca gaaacgaacc aaaatgtttc tgcccttttt ttcttatacc
     2101 acatccataa agccgcacaa gcaaataaaa tgatgacggt aagaggatta ccgaatttag
     2161 tcataaagag ttggtagctg ttcagatcag gataagaagc tctgacaatg cctgtaaacc
     2221 actgatcgaa accagaaagc cacgacagat ggaactttac gatccagccc aaaatgaaaa
     2281 acaggacaag aaagaagcta ccagccacct gaaatgatgt tttatatttc atacaattcc
     2341 tcctttgaag caatgactta ttatagcatt tttggtccag cgaaaaaaac ggtaaagaga
     2401 taaaaccaat gtatgattcc tctttgaata accaatattc ttataaaaaa aataactgta
     2461 atcggaaaga cattttttgg taatgcaaat gaaatgttag ggaaataaaa tcagtgtttt
     2521 tcgaaatcct tttaaacaag aacgtagacg taggaagaac ttgaaaatga acagaagttg
     2581 ttcattacgg taaattacaa tatgattaca aaaaacaaca aagctcttaa ataactttta
     2641 ataaatatta agagaagtta taattattaa taatagataa attgaagttg gaggatagaa
     2701 gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac gttttcgaaa
     2761 agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc
     2821 attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat
     2881 caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg ctcaagccaa
     2941 cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt ggggcagtag
     3001 tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct atcaaggaca
     3061 aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata aaaaggagcc
     3121 tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg tacttagaaa
     3181 tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca cgaaatccta
     3241 tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt acgcaggcaa
     3301 attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag ctagcggaaa
     3361 cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta gtactggcgg
     3421 ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt atactgtcca
     3481 atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg atcaattgat
     3541 ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga caatcaaagg
     3601 tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaact cttcaggaaa
     3661 tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc aatctggtga
     3721 ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga ttgaatggaa
     3781 caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa atggcggttc
     3841 ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct ctagttcgtc
     3901 cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag ccggagactc
     3961 tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc aatggaacaa
     4021 tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca gtggatcaac
     4081 aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca gttcagttaa
     4141 tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat ctgtttggag
     4201 cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata atatcaaaaa
     4261 caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg caaatacaaa
     4321 tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg ctacgacaag
     4381 tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt
     4441 agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata tcaaaaataa
     4501 tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat caacaaacca
     4561 acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg tttggagtgt
     4621 tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata ttaagaacaa
     4681 ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt cgaagaaata
     4741 acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg acaacacttg
     4801 aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga gaatgtgcac
     4861 ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg
     4921 gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt
     4981 agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc ttttgtgaag
     5041 tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt ttttcgttat
     5101 atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga gttacaatca
     5161 taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca
     5221 tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc catccggtca
     5281 agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa
     5341 aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc
     5401 tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa aaaatatcga
     5461 aacattccga cgccaaatca attctttagg atttagttat gactggaatc gcgaagtgaa
     5521 tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc tttatgaaaa
     5581 aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag gaacagttat
     5641 ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa
     5701 accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat tggatgatct
     5761 agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga tcggtcgttc
     5821 agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata ccgtatttac
     5881 gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg aactagatct
     5941 tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata tcgaagagac
     6001 agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag gagtatttac
     6061 tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga tcgccgatta
     6121 tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg atgaacgtga
     6181 ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag gtggagatgt
     6241 tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc tagacggcat
     6301 gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa
     6361 aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt actggggtga
     6421 accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag aagaagagct
     6481 accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag aatctccatt
     6541 agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg
     6601 cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc gttatatcga
     6661 ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc taccagttga
     6721 catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac gattctggca
     6781 taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa aactatacaa
     6841 tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt
     6901 caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt
     6961 catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa
     7021 attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat
     7081 cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga aaaaagtgac
     7141 agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg tctttatcaa
     7201 tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg ttcaactact
     7261 cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa atgatggtgg
     7321 tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag acgaagtaga
     7381 agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg
     7441 aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat atattgaagg
     7501 caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta
     7561 attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga
     7621 acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg atcaaaaagg
     7681 ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg gtcttgtaag
     7741 agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga tgtataaact
     7801 acctgaaatt gagagtacat accaaattca taaatttttt attagtggga ctagtgattg
     7861 tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat ggatcaagaa
     7921 ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga
     7981 gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt gctttttttc
     8041 ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc tgaagggatg
     8101 caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac aattgttgtg
     8161 cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg tcatttcgat
     8221 caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc tcaagatgat
     8281 gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca aaaaagtgat
     8341 tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga aagcaaatca
     8401 aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag atgggaaaaa
     8461 agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt cactggtttt
     8521 ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc aaaaatgtct
     8581 tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac gcatatacaa
     8641 aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac aacacaaaag
     8701 ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac agatagtggt
     8761 gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga atacaccctg
     8821 acaacggggc tgattcctga aggttattgg atggacaaca catatgatat ttccgccgat
     8881 ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc aagagataat
     8941 ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg ttatcgaaaa
     9001 gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg gatgtctgca
     9061 gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa aagtggaagt
     9121 acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt aggttttgtt
     9181 tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa agaatcagtt
     9241 ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc accaaatgaa
     9301 gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa aaaaggacca
     9361 atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg
     9421 caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga gattgtgagc
     9481 ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc atatttgttg
     9541 aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt tgaagtatag
     9601 cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt
     9661 attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga aactgaagga
     9721 gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc actagtacat
     9781 ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt agataatgtt
     9841 tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg tgattggagt
     9901 agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag gatcatgtct
     9961 gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca
    10021 gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga agttgcttca
    10081 tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa taattgttgc
    10141 tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc gtctggcagt
    10201 gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg
    10261 gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag ccaggtattt
    10321 tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt aatatcaaga
    10381 ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag attgactaaa
    10441 gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg ctttactgaa
    10501 aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac atttttgaaa
    10561 gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg aatttcatct
    10621 gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg gatcgagttg
    10681 atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg aagataagct
    10741 tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa taccgcactt
    10801 atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca gttcataaat
    10861 tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc agcttctgca
    10921 tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc ttccattcga
    10981 ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt tttgttcgca
    11041 atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa agtaagcttg
    11101 ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga agtaaaaaat
    11161 tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt tgtccgacta
    11221 atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt gaattttttt
    11281 gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat tgtccgttcc
    11341 tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa gacagggaac
    11401 aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt tcttatttct
    11461 gataagagat catcgatccc gattcccatg atcaagggca tggctacgac taataggttc
    11521 ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt gatcattttc
    11581 caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt atcccttctt
    11641 tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac agtagttctt
    11701 catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta tctgcttgtt
    11761 ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg tgtttatgaa
    11821 gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta tcatcgaata
    11881 catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc
    11941 ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca
    12001 ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt
    12061 catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga
    12121 cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa
    12181 gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt
    12241 ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa
    12301 tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag
    12361 ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt
    12421 cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa
    12481 tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta
    12541 cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca
    12601 aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata
    12661 ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt
    12721 cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca
    12781 atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt
    12841 tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta
    12901 gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta
    12961 agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc
    13021 ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt
    13081 tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt
    13141 aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa
    13201 ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta
    13261 attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag gtaaggagag
    13321 cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt
    13381 cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa
    13441 actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact
    13501 atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag
    13561 tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga
    13621 tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa
    13681 aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact
    13741 ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt tgaaattgat
    13801 gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc
    13861 aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg
    13921 gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag
    13981 tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg
    14041 tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag
    14101 agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac
    14161 gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa
    14221 ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa
    14281 ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt
    14341 tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat
    14401 acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata
    14461 attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta
    14521 tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa atacattgtt
    14581 gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg aataaacata
    14641 ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata c
//
LOCUS       DAYYNG010000066        14477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000066 DAYYNG010000000
VERSION     DAYYNG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.066"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1462)
                     /locus_tag="ACHBFA_001583"
     CDS             complement(98..1462)
                     /locus_tag="ACHBFA_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9395074.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1459..3018)
                     /locus_tag="ACHBFA_001584"
     CDS             complement(1459..3018)
                     /locus_tag="ACHBFA_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395075.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3074..5554)
                     /locus_tag="ACHBFA_001585"
     CDS             complement(3074..5554)
                     /locus_tag="ACHBFA_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395076.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5538..5873)
                     /locus_tag="ACHBFA_001586"
     CDS             complement(5538..5873)
                     /locus_tag="ACHBFA_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395077.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5887..6921)
                     /locus_tag="ACHBFA_001587"
     CDS             complement(5887..6921)
                     /locus_tag="ACHBFA_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9395078.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7011..7691)
                     /locus_tag="ACHBFA_001588"
     CDS             complement(7011..7691)
                     /locus_tag="ACHBFA_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9395079.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7861..8820)
                     /locus_tag="ACHBFA_001589"
     CDS             complement(7861..8820)
                     /locus_tag="ACHBFA_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9395080.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(9041..10144)
                     /locus_tag="ACHBFA_001590"
     CDS             complement(9041..10144)
                     /locus_tag="ACHBFA_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM9395081.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(10366..10836)
                     /locus_tag="ACHBFA_001591"
     CDS             complement(10366..10836)
                     /locus_tag="ACHBFA_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM9395082.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            11093..12412
                     /locus_tag="ACHBFA_001592"
     CDS             11093..12412
                     /locus_tag="ACHBFA_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM9395083.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            12629..12978
                     /locus_tag="ACHBFA_001593"
                     /pseudo
     CDS             12629..12978
                     /locus_tag="ACHBFA_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(13250..13540)
                     /locus_tag="ACHBFA_001594"
     CDS             complement(13250..13540)
                     /locus_tag="ACHBFA_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395084.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(13748..14440)
                     /locus_tag="ACHBFA_001595"
     CDS             complement(13748..14440)
                     /locus_tag="ACHBFA_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9395085.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         4125 a         2918 c         2538 g         4896 t
ORIGIN      
        1 ctcaaaaaat tcttccgcta tttcattata tgaatttttt attctcgatt gattaatatt
       61 tattatatcc ccacacaata aaagaccgtg atatttctca cagtctttta tctactctat
      121 tgctatttgc tctcttagct agtactttaa aagctaactc attgaggtag tgaaaacgag
      181 aggtgtattc agacagaaat ccaaaatcct caactgctag ctgtggaatc gtagttttgt
      241 gtttcacgat atccgcaagc aggttcaaat ctgataataa gaattcttgg cttatcagct
      301 gtccgccaat tatttctaat gtatccggat ggtagatcag ctttgcattt actgttttgt
      361 tttcttcgtc gtattgtgga aatagatcaa attctcctga acacgaaccg gtattttgtt
      421 ccaagaatgg cgcttcatct tctgtcagcc ctacagttcc taaaaagtag tctataatga
      481 ctgctgctgt tatcttttgt ctgaggtcgt aagcggttgg ctgaccactc aaagttagcg
      541 ctactgctct tgccatatgt acagctcgag taactaaagg taaaaagaga tgccgtttat
      601 ttttgtcaaa actgactggg acgatatctc caatcgcata gatgtccgga tcacttgtct
      661 gcagatattc attgactatc acagtctcat cctcatacaa agccactttt tcttttaaca
      721 atgtcgtatt aggacgagtt tgggcagaaa aaatgaccat atctgccttt attgtttgag
      781 acagaagttg tacggatact actttctctt ctgatttttt ccaatcgatc aagaattcat
      841 ttggataaaa atttatttgc ttagtttgca gctgattttg aaaactgttg atcatatcct
      901 tatcgaaata tcttgataaa acattcggca tataatcaac tagatgaata atgtatcctt
      961 tatttctcaa agcatctgct aattccaatc caatgtaacc agcaccgaag atcactactt
     1021 cttttgcttc ttttaaagcg gcaagtactt gcttagattg cactagattt ttataggtgt
     1081 agacattttc agcttcaatt tgtttataca aggtgacagg agaggaacct gtcgctacga
     1141 ttaatttatc ataggttatg gtctcgtttt ttccttcttt ggatgcgagg atgagctgat
     1201 gtttgtctgg atcaagttca gttactctcc attcggtgat cagttttgcc ccactttctt
     1261 gaagagtccg catgttatgg gcaacttcag ctaaactacg tatctttcct tttaaataaa
     1321 ggttgattcc atttgaaaca tagcctagtc catctttatg atcttcatcg atcagtaaaa
     1381 cttctgcttt cggttgaagt tttcttagat ttagggcagc tgtgatgcct tcatgggaag
     1441 caccaatgat cacgattttc atgttttatt catccttaaa ttcaattttt ctaaataaca
     1501 ttcttttaaa aattggttca aataggggaa atcataatta tattcgttgc tagtgaatac
     1561 aaaaacttta gcggacttct gtgacgggta ctctttcaat tggctagtca aaatgatatc
     1621 atactgcttt tctttctcgt attcgactaa atcatatttg atcgtattca gcaattgttc
     1681 ctggatgatt tctttcacga tatccgccat tagcctttca taaggaagat cataagcaat
     1741 ttgcagacgt aattctaact gcttgatcag cgtccgtaaa atagatgcat agcgagcaaa
     1801 taattcaaag cgggcatttt cctcgtctac ttcagtaaaa tcaacaatag caaatgtttg
     1861 tctgaccaat ctttcacata aaaccaggaa ttgtttatct tctacttcag ttaaccgctg
     1921 ttttaatcct tcttggccaa aaatattcat ccatcctcta aaataggcaa aatgagcgtg
     1981 attttgcatc actgtcaacg caatcttttg cgaaatctct tctggaaaaa cataaaaaga
     2041 gaaatattct tctatcgatt gtttaaacgt tcgattcaat tgagctacta cgtcgtcttt
     2101 gtatttttcg cttatcaaaa actcctggac atgcgagtta tgtgtatcta atgtaaagtt
     2161 actgatcatg aaaagataaa aaaccaacgt ttcattttga ttccaaggaa aggcatattg
     2221 aaagaagtaa cgggtcaaaa atgtatggag gtccaataaa aattgtgtcg tttgaaacaa
     2281 tccaacagga agattgctat aatctggttt ggattttttc gtacagcgca ttcgcttctt
     2341 cataatggaa agccatattt caaatttcaa tcgtccttct tctgtaatag caaaactaaa
     2401 ctctttttgc agcagatcta aaaaagtgtt tcctgataaa tcactgtatt cttttttgtt
     2461 ttcaagatag ggacgtccaa accaaaataa ttggaaataa aaataacgaa tttgactttc
     2521 tggaccggtc agccgcccgc tttttatttt cagatgaaac tcatttaaag aatcatttaa
     2581 ttccttcact ctgcgagaca aagtagccga actgatatta agttccatga caagtttctg
     2641 ccgatcaaat gagccatttt ccaacagata aagaagtatc tgatattttg tagaatagcg
     2701 caagtaacaa tgataataaa catcgactgc tgctatattt ccatgtctga ttaataaaat
     2761 attcccttct tggataacaa tttccaattg tttgatattt tccaagttag cttgtaaata
     2821 aaacagatct tctttcaagg tagcccgatc aactgataag cgggtaagta cgttattaac
     2881 ggaaacctct cctttttcct ctaataaaag ttggaaaagt ttgatttgcc gttgttcatt
     2941 tttttcaagc aaactgaaaa gttccaagtc cactccttca agcatttctg attttaacag
     3001 cagaagtttc cttctcatcc ttgaaaaaga ggatctgtga cagttatcac agatccatac
     3061 aaattctaat cacttagctg gttggcccag ctgtcagttc ccatgtgacc atgccttgat
     3121 acgttgcgcc aggttgagca ttcgtactgc tatttacctc taagagtaaa ccatcggtat
     3181 cgggaaagtc aacgagaacc tctcctttag ctgttccatc actctcataa atcaacgtgt
     3241 tttgaccttg ctgaatcaat tgctttgatc cagaatgatt gaagtataat tttgttccgc
     3301 ttaattcttg accttgatca ttcttaaata aagaagtcaa tgtacctgtc agttgccaag
     3361 gagacgtgct tggaagccga gtatcccgca catcaaaaga aaaatcgcct tttgattcat
     3421 aataacttgt cttcaacggt ttggggagat cgttaccaaa tgtaaaatca tctggtacat
     3481 taactaatgt taattcttgt ttggggacaa gaattgtttt agcagatgtt tggacaactg
     3541 ggttaggaag cttagcacca tctgctaatg taatattgct ggaaagtaca gctgcctttg
     3601 tcagtatgct gacggattgt aaagaagtat ctacagtatt taattgatag ttgtaagtaa
     3661 gatgaatggt atctcctatt ttgctcaata gatctgctgg gacagaaact ccttgttgaa
     3721 tgccggtatc tgcgatagtt gtaccattta attttaagga accattgata taagtagtat
     3781 tttcaggaag tgagatcgtg atttttttgt cggttatcgc atccccagat tttgttaacg
     3841 tgaatgtcac attttcttga atcgtatctc ctgatagagc agacagggga tctcctgtct
     3901 caggttgtct gctaaggttt tttagctgac tatctgcact cacagatgga aaattagcca
     3961 ctgttatttg aggaagagtg gtgattccag cttgttcaga ctgccatttg tacgcaaaag
     4021 acggatacat gttagtaagt acatcgtcca ccaagcgata atcaaaataa tattgccctg
     4081 aagccaatgc aggtagtgta gaagtagctg tatttacacc cccgttattt gattggatcg
     4141 tttggaaaag ttgatctgtc catcctgtcg ctaatttttg ggttcctttc gaataaagcc
     4201 tgtaatggat cgaacccttt tttagttcag ttgctgacca attggccgtg acttggtacg
     4261 atgcagaaga aaggagcttt ccccactgac tatcggctgt taagtttgtt ggtaaaaccg
     4321 ttccgtctag cttgtactgc tccaatacct ttaaagcaac aggagaaagc aatccgcctt
     4381 gcgcatcttt cccactgtac acgatattgg ctgtctgatt caatccactt gtgcttgctg
     4441 gagtatgagt cagtattgct ccttgtgtaa cgttttttcc tgtaaaatag ttgactacgc
     4501 gatcaaagcg actaaatggc aactgatcaa aacgtccttc agtcagcccc atcgtcaatg
     4561 tccgactatt ggtagagcct ggaaggttct ggttatttcc atccacgtat tctgtggtat
     4621 cagcaaatgt attatatttt cctgttgaat cacttggtga gatgtaacta ttccacgtat
     4681 ttatctctgc acttgcagaa aatgcatatg ctcctggatt caagtttccg ccagttaatt
     4741 taggataaaa tctacctgca gctggagcta aaatcgaatt gaacatacga tcttggataa
     4801 tattattcga atcataaaaa tcaacagaaa aaacggcctt tttagacata gaaggttgta
     4861 atttaccctt ggtcaaaatt ttgtttggac tcgtatcata tttatctccg ttttctgtca
     4921 tcttcgcgac attagctgta acatcaaatt gaagcgggat atcgtccacg ttgaattcgc
     4981 ctcgcgtaat aatagcaata atatctgtca atagagtatt tgtatcccac gaccccatcc
     5041 caaaacgcaa ggtatggctt cctgctgata ataaacttcc gctagtaacc ttgatggggg
     5101 caccggcaat atacaagtag gcgttgttcc agtcaattgc ttttaataca tcactgcttg
     5161 atacatgttc cggcaaattg atgatcgcca caaatgcaaa tccttgtgca gtgatattca
     5221 ccaaatcaag tacatttgtt agtttcacat ggctttccca atgaaaacgg accgtgccac
     5281 ctttccaagg atcatttgaa ccatcgttca aataagtagc agtcaattta ggcaattggt
     5341 tagccccagt tagttggaca agcttcccgc cattgactgc actacgtcca ccatttggag
     5401 gtacgatatt ttcatacgtg tatggatcat aagaaatggc aggaggcgtt gctccaacag
     5461 cttctgccga aggtgaaaag ataaatatac ttaaaacaaa aagcgaagta aaaaagaagg
     5521 agcgaaaaaa tgaaaattta ttttgtagca acatgttcat cctccttttc ctttcttctc
     5581 cataacaaca accacgcaag aagccataat tctataccaa tgatcaaata aagaaaacgg
     5641 atcttttgtt cattcgtctc cgggaatacg ccatgttgat tagattcttt ataaatcggt
     5701 agctgtgtct gctgttttga tgcgccattg actgaaccac ttggatgcgg aattggactt
     5761 agatcataaa atgtaatcgt acctgaactt ttggtatctg cttggactgg ctcttgccag
     5821 ctgaagaaca gcaaacttgc aataatccct cccaataaga tcgatgtttt caacgaaatc
     5881 acctcatcat gttgtttctc cttttttctt tttcttgtgg acccacaaaa acagcaaaat
     5941 caaccataac actagaactg cagcaaccaa tcctgcataa agccatggga aagattgttc
     6001 ttgtactgca aaaacggaat tttcatttac tttttgtgct ttttcttttt tgatcgtaaa
     6061 gttttgtgta aatcgccatt cattactgtc gcctgatttt gctatgattt ctgcgcggta
     6121 atcgccggct tttgctttat caccagctaa atcaagattc aagtgaaaat tcgaatttgg
     6181 tgcaactaaa aatgcattag acgtattttc aatataaagc tgattttcat aaaagatctt
     6241 gctcgtaaat atcaaattgc tgataatcct aggctgagga ttttggaaac gaagctgaac
     6301 agttggcttt gcgttgacct cgtccaaatc tcctgataaa agtttcagat ccggttctat
     6361 cgtctcttga ctttcttgta aaacgactgc gagattataa gcaatacgat ttttgatttg
     6421 tgctccttct gtagcatttt ctgtttcagc ttcttcttta ggtgaaacat gaatacccgc
     6481 taatacacgt cccttaaagc tttttgctgg gattttcact tctataggta cagttgtttc
     6541 tgcatgtgct gatacattga tttccttttt cgcaatgagc aaaacgtcac ggacatcaaa
     6601 cggcaatgtc tcatctaacg ttgggttttt ttgactataa tcgatcgttc caccatcact
     6661 cgttaccgcc ggattgacag agacctcaaa tgttcgttct tcatctgaag aattgctgat
     6721 aattacttct agtgtctgat tttgttccgg tgccactaat aaatcaaaat aaccgattgc
     6781 tttatctatt tggttctctg gaaataccgg tttgattcca aagtctcctg ctgaatcctt
     6841 tgcttggctg attgacggaa aaatgaaaaa tagacttaaa atagaaaaaa acgaaacaaa
     6901 cagtcgttta ctttgattca ttgtccacca catcctttca acaacaactg cggtaaaaaa
     6961 tccttcacct atagtatttt caccgcagtt tgttgaattt atttcatact ctatgctact
     7021 gggccagcca cgaggttcca agtcaaatca gccgtgaatg ttccagctgc tgctttcggc
     7081 actttcagtg tgatatttgc tggatcataa acagaaagcc atgttcccag accttgatct
     7141 tttgctgctg taaatattgg tgtcgctgca cttgctttac tcaacccagt tacagtttga
     7201 ctagttggtc cagtggattc gttatctgac gtacttttaa cagtggatgt tccaaaatct
     7261 aattcagcgc cttgcaaaac ttgtgaaccg tttacaaaat ctgaaaccga aactgtcacg
     7321 ttccaccctt gtgcttggcc atctgcacct acacctcgac gatctgatac ttggagataa
     7381 ggtgtatcgt tttttgaagc gtctacttga tacgtttttg ttccgctttc aatctcgtgt
     7441 gtaccaaatg gcaattcggg aactacatcc agtgttaaag gacctgtctg gccagttgct
     7501 ggattttccg gatctgctgg ctctactggt tttgaaggat cagtagggtt aagaggctcc
     7561 acggtatttg ttggttcagt aaaggtaata tctccttttg aagtcgcttg accctcagca
     7621 aaagctggtg ttgccaagcc gcctagaaga agtgttgaaa gtaataatgt cccaatcatg
     7681 ttctttttca taataaatca ctcctttttc ttagtgacct aagtatagta gagacagtat
     7741 gcaaaaaatc ctcatgaatt catccgctta cttgaaatta tgaccctttt tgtataaaag
     7801 agaaaaaaac aataaaatcg tttacatgaa tattcttcct tttttattaa gtttcgtttg
     7861 tcaaattaag ttagacaaat catcttttgt gacgtaactc aaaaatactt ccattggtgt
     7921 ccgatagtgt aatgattttc taggaatatg atttcgtttc gatgccaccg attggataaa
     7981 ggtttcagat acaccattaa aatccatagc tttatacaaa ccatctttac gtaataagcc
     8041 atttgaatgt tcattcaatc ctcgttgaga aggtgttcct ggatccgcaa aataaattga
     8101 aatatcgttg gtattactaa tctttttcca atttgagaat tcttttccac aatcaaatgt
     8161 gatggattta aataagtttt ttgggacaca acgaaaccat tgattcagcc tattttcaat
     8221 gtctttagcc tgtctgcctt cgggtttcaa ggtaatgatg actttagata gccgttcaac
     8281 taatgtgatg accgcgcttt tatgttttcc acccacgata gtgtcacctt ccaggtgacc
     8341 aaattcatga tcaaactggg tatagtctgt ctttctgtct cggattgacc ggcgaaaccc
     8401 ttgttttcct cttttttctt tatgaccgtt tggttttctt tttcccttca ttggtaaatc
     8461 gtgagtgtca aaggttttat ctttaaacat tcgatacagt gtgcgtaccg aacaagcaat
     8521 aggaaactct gctcgaccaa taaggacatc aggagcccac ccttgaacaa cctttttttg
     8581 aatgtattcc tgttgctcat caggtaaaac aatcggatgt cttccgcagt tacgtttgtt
     8641 tttcttatat tgtttatagt agctcattgc gcttccacca gatttaagaa aacgatagac
     8701 cttataaata gtttgtcgag aacgttgtaa cagattggct acttttgaaa caggttgtga
     8761 ttgattgaaa taagcttcta tcattacgag ttcagttggt gtaagatggt tataggtcat
     8821 ttgtactcac tccttataat tctttcgtcg gaactatttt gagtgtagca caaatgactt
     8881 tttttagttg tctagcttaa ttttacaatc ggcgaagtat gaaaaaatat ttcaatttta
     8941 ttttttcgtt tcaaattatt ctattagtga catccttgaa ctataaaaat aggagtacgg
     9001 catcatctgc cgcactccta tttttttcgt catttgtttc ttaaataaaa cgattctcac
     9061 gcaattttat ttttttgata tcttctactg tttgtgttcc agctaattgc atgaccattt
     9121 ccaattctgt tttaaagaaa tcaaacactt gatggactcc tgtacttcct cctaaagaca
     9181 atccataaat aacgggtctg ccaatagcaa caagatctgc acctgatgcg attgctttga
     9241 aaacatgctg tccgcgtcgt acaccgctgt caaacacgat tggtacacgt ttatctactg
     9301 cttctgccac atattgcaac gaatcaaacg ctgctggacc accatctagt tgacggccac
     9361 catgattaga tacccagatt ccgccagcac cagattctaa tgaacgatag acatcttctt
     9421 ctgactggac acctttcaca tagactggca aatcagaatg agcagcaata aattctacat
     9481 cttttggact aagcttctgt ttggaagatt tgtacacagc atccatcgtt tggcctactc
     9541 ctgattgata agcctgaacg atcggcattg gcagtgggaa ggtaaaccca tttctgcggt
     9601 ctgtttcacg gtttccgcca actgttgcat ccgctgtcaa tacgattgct tttgctccat
     9661 tacgcttggc cacttcaaga atatccaaat tgatcccatc atctttgctc atataaaatt
     9721 gaaaccattg cggtgctttc tccccgcctg cttctcttat ctcttctaat gtacaagaag
     9781 cgtaagaact tgctgtatag atcgtcccaa aatctgctac accttttgca gaagctttct
     9841 ccgccttcac atgagctaag ccatgagcag caactggtgc catgatgatt ggtgcagtca
     9901 gcgtttcttt gtcaaaatgt gtagttgtat cgggtaattc tacatctctt aatacatgag
     9961 gaataatcag ctgatgattg aatgcccgtt cattttcttg ataagtaaat aagtcgccag
    10021 caccactgct gatgtaacca taaccaccag ctggaatcac ttgtgaagcg gctatttcta
    10081 aatcttccat attgataaaa tccactattc cttcatttgt acctgcttga tacgtttttt
    10141 ccatttcaat gccttccctc tataaaaaat tgaatgaata atatgatagc gtttacacac
    10201 tttatattac tcttccgtat tgatacaggc aaataatctg tttacatttc agtcattcca
    10261 gttgttacta ctctgaaaga aatgtttaga acacaaatag ataaaaaacg atgggatttt
    10321 gacaaaagtc gtgtcaaagt cccttttacg aaatactgtg gatgcctatt gaaataagtg
    10381 cgggttgttt ctttgtattt caatcgtttc ttgccattct tcgtttaatt ttggattacc
    10441 gttttcttcc ttgaacgcat aaggtcccaa tccaaaaagc tcagttagta tttgattcac
    10501 gattccatat agctcggtgt gatcggacac atgattattg aagatcatct cattatcacc
    10561 acgtttgaat ctcacattcg ggctctcatg atgaacctct atgcagtttt cccctttttt
    10621 taactgacgg atttctgaaa acatctcata aatatcttct ccaataaaac cttcagcaag
    10681 cgccagactg tccacaagac cgcctactgt caactcagag attatcgctt tgttggcacc
    10741 aagctttaat gtatagcttg ttgcttctac tacgccgcat gcattgcaac caccttctga
    10801 tagttcgctt tttctgatca attgaacttc tttcattttc tgttcctcct cttagttttc
    10861 tacttatgta ttttttatca cctgacgtaa gtgtatttta tcgtcacgat cacgtgattt
    10921 attcttcaga gattctacct gaataattca tacttttaat taaaaccatg tgaaactgcc
    10981 tcacggcagc ttacaattac tttttcatct tcacccgtta agtgatgaaa aaagaacccc
    11041 tttctgctat ggttaaagtg actaaaccaa ccaaaagaaa ggagttcttt tcatgactag
    11101 cttacacaaa aaccaagtaa aattcaattc aaatatcact atttctcata caggtggtca
    11161 attatcgagt gattcgggtc tcgtcctagt gaaagagctg atgaacatct ttggtttttc
    11221 tgaactggct aagcaacaca ttcacattga agacgaacga gcgtatttta ctcatgacaa
    11281 cttatccata cttgagcagt tcattatgca attaattgct ggttactcag ctgattctgc
    11341 agctaatctc ctgagacaag atcctgtgtt taaagctgtt ttagatagga aagaactcgc
    11401 ttctcaatct tcactttccc gatttttaga tcgactatct gaggagaata tccatgaact
    11461 tcaagctttg aaccaagaac ttatcgataa agcacgcctc atccgtaatg atacggaatt
    11521 aatcattgat ttagattcga cccattcaga tacatttggt caccaagaac aaacgaatta
    11581 taatacacac taccaaacct atggttacca tccattggta gcttttgacg gattgactgg
    11641 tgacttctta aaagctgaac tacgttcagg aaatcaatat acatcaaaag gcgtaaagga
    11701 gtttctcaca cctttattag agcactataa tcactctcta ccaaacactg acatcttggt
    11761 tcgtggagac agcgggttcg ctacacctgg tgtgtatgat tcgtgtgaat caaaaaagag
    11821 tcattatgtt attcgactga agaataatcg tagactaggt caaatagctg agaaatcagt
    11881 actttatggc gataatcaaa agtgggaaga acgagaagtt cagtacttct cgaccactta
    11941 tcaagcacaa tcctggtcac aaagtcgtcg cgtgtgtata cgctcaacac gtgaagcggg
    12001 cgaactactc tttcgacatg aatttatcgt gacgaatcta tcagaaaatg tttctcctga
    12061 taccatcttt tctatctatg ccaaacgtgg cacaatggag aacttcatta aagaagcgaa
    12121 agctggcttt tactttgaca aaacagacag tcctcgcttt ttggagaatc atgtccgaat
    12181 gatgctcagc ttgatagctt acaacttagt caacttctta agaacgattg gctttgaaga
    12241 agtccaaaag ggaatgacca ttcattctat tcgattgaag tttctgaaag ttgctgggaa
    12301 attagtccaa acgggtagac gagtctatct caaattatct agttatcatg tgtatcagaa
    12361 tgaattctac agggtctttg ctcgcctgag gcgagccagt caatggatct aatccaacaa
    12421 tctaacgatt tttttactgg gaagttatcc agggagaagt atgctcaaaa tgccttacac
    12481 ccaaaattta ttcctcaata tttttgaaca gtgaatgttt gaagtaactt tttagtcaaa
    12541 atcagttgat agggatatta gtgatacata tttcaataaa atgtgccaaa gaattaaagc
    12601 tatgaattat tcaggtttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat
    12661 ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac
    12721 ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt
    12781 gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt
    12841 ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac
    12901 gactatgata atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc
    12961 tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc
    13021 cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct
    13081 caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata
    13141 tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca
    13201 tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc
    13261 tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt
    13321 tgggtttccc taacacatga taggatctca caacaaattg ctttctatta ttataataac
    13381 ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag
    13441 caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca
    13501 aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat
    13561 tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg
    13621 tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa
    13681 aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta
    13741 agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt
    13801 ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag
    13861 cagttgcttc gtctaatttc ttttgtgtat ctgttaactg agtttctaca tttgtttttt
    13921 cttgtgtcag tgttgcgatt tgttgattca aaccatttac agtattttgt aattctgaaa
    13981 gttttgcttg tgcttcttgt agtcttgctg tccaatctgc attcagatga ttgatttcat
    14041 catttttttg gttgatctca tctagtttga cattgatttc attttgtttg ctttgtagtt
    14101 gtgcatcctt gctgactaat tgactttcta agttttgttt ttctgctgcc gattgatttt
    14161 tttggttttc taattcattt acttttgttt ggtattcttg gacatctgcc tttgaatctt
    14221 gcaatgtctg ttctttttgt gctagtcgag cggtaagatc tgtaatcgct tgctctttat
    14281 ctgtcaatgt atgatcaagc tgtgtcagat tctcattgat tgtatcaata tctgtctctc
    14341 caccccacaa aataatttca tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa
    14401 atgcaacgac aatcccacca gtaataaatt tctttttcat ttgcatagct ccttaatcca
    14461 atacttttac atgctaa
//
LOCUS       DAYYNG010000067        14054 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000067 DAYYNG010000000
VERSION     DAYYNG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.067"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..249)
                     /locus_tag="ACHBFA_001596"
     CDS             complement(<1..249)
                     /locus_tag="ACHBFA_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9395086.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLI"
     gene            complement(728..1447)
                     /locus_tag="ACHBFA_001597"
     CDS             complement(728..1447)
                     /locus_tag="ACHBFA_001597"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9395087.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1496..3142)
                     /locus_tag="ACHBFA_001598"
     CDS             complement(1496..3142)
                     /locus_tag="ACHBFA_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM9395088.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3321..3911
                     /locus_tag="ACHBFA_001599"
     CDS             3321..3911
                     /locus_tag="ACHBFA_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9395089.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            4003..5508
                     /locus_tag="ACHBFA_001600"
     CDS             4003..5508
                     /locus_tag="ACHBFA_001600"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9395090.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5592..6944)
                     /gene="celB"
                     /locus_tag="ACHBFA_001601"
     CDS             complement(5592..6944)
                     /gene="celB"
                     /locus_tag="ACHBFA_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9395091.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6944..7462)
                     /locus_tag="ACHBFA_001602"
     CDS             complement(6944..7462)
                     /locus_tag="ACHBFA_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395092.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7478..7804)
                     /locus_tag="ACHBFA_001603"
     CDS             complement(7478..7804)
                     /locus_tag="ACHBFA_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM9395093.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7817..9796)
                     /locus_tag="ACHBFA_001604"
     CDS             complement(7817..9796)
                     /locus_tag="ACHBFA_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9395094.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9817..10131)
                     /locus_tag="ACHBFA_001605"
     CDS             complement(9817..10131)
                     /locus_tag="ACHBFA_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM9395095.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10299..10592
                     /locus_tag="ACHBFA_001606"
     CDS             10299..10592
                     /locus_tag="ACHBFA_001606"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM9395096.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10670..11782)
                     /locus_tag="ACHBFA_001607"
     CDS             complement(10670..11782)
                     /locus_tag="ACHBFA_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9395097.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11935..12783
                     /locus_tag="ACHBFA_001608"
                     /pseudo
     CDS             11935..12783
                     /locus_tag="ACHBFA_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13136..>14054)
                     /locus_tag="ACHBFA_001609"
     CDS             complement(13136..>14054)
                     /locus_tag="ACHBFA_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9395098.1"
                     /translation="REGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQAPTGYELDAT
                     PIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSG
                     LTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLT
                     PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4375 a         2878 c         2409 g         4392 t
ORIGIN      
        1 gatgagaaat gcttgatccg aagaaaataa aaatttgaaa ttttcccaac ccacccaagg
       61 actttccctc aagctagcca agaacccatc tgctgaaata tgaaaatttt tgaatgctac
      121 gacattcgcc attacaggta tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc
      181 catcaggaca agtgctttat aacgccatag attcataaaa aaatgattga acccctttcg
      241 ttttttcatc taaaaacccc tttcgattca ttcgcaacgt aattatagcg ctttcaataa
      301 tgagaataaa taaacaaagt atagggaaaa tgaaacaaag tataggagag gttgtgaaat
      361 cagcgttttg gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg
      421 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaatt
      481 cagcgttttg gcctgagtat tagggagatc gtgtccttac tatctaactc tgagtcacaa
      541 ggaacaatgg ggactgtaac aaaagtcttg tcacagtccc ttattccgaa caaacggtgt
      601 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaaagccat ccgaaagtca
      661 gacggacaag tctgcttttc agatggcgct acaactactc tgcttattct tttagctatc
      721 cattcaatta tttttctctc acttgttcta gctcttcttt ggtcatgggt cgatattgcc
      781 ctttattcaa atctttaggc agccaaaatt ttcccatacg gattcttttt aaatataaga
      841 cttcttttcc tactgctttg accattcgct taacttggtg aaactttcct tcatgcaaaa
      901 tcagacggat aattgaagtt tctgtctctt gatctacttg atcaatttgg agttttgccg
      961 gcaatgtctg ctcatcgcca tcaataatca gtccttctgc aaataaacgg atatcttctt
     1021 ctgtcattat accgctaacc tcagctagat attctttctc cacatgtttt ttaggtgata
     1081 gtaactgatg ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat
     1141 ctaatcggcc gactggaaaa agatcttccc gataatcctg gtcttccaat aaatcgatta
     1201 cggtttgatc atgattatcc tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt
     1261 agtaaaaatc tttttgatac gtaatgattt ctcctaaata agtaaccgca tcttcgaatt
     1321 catttacttg cgctttatcg cttttcacca cttgtccatt gacttcgatc gttccttttt
     1381 tgattaatcc tttgacttct ttgcggctgc caagtcccat atctgctaaa aacttatcta
     1441 aacgcatgcg tttcccccat ttcaaaagag catcaatcta gctcttctga ttcacttatt
     1501 ttatttttaa tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc
     1561 ggattttcaa agctaaataa ccatacacta aagcccctat acctgctact acaagaatca
     1621 acaatagaga ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga
     1681 tccacgaagc caaaagcata atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga
     1741 caaaacttgg tttaaagcgg gctacttcat ggattctctt taagatcaag gtacaagaca
     1801 cagcaaatcc gatcattgtc gccaataaag ggccgtatac ttcaaataaa cgaatggctg
     1861 ggtattgaag aaggagtttc actagaaacc caataaccaa ataattaatt gccgccttgt
     1921 tttcaaacat tccttgcagc atgttggaaa ctagcatata tagtcctagg aagagtccta
     1981 caaaacttgc ttggatcagt acattacttc ctagagaatc cggtgtataa aataatgtat
     2041 ttagcggata ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca
     2101 actgtaaatt tccgcttgtc aattttgcca gacctactcg atctttcaac gtaacagctt
     2161 ctgtaatcaa cggtaaccca gtagacgcga tcgatgttgc caatgcaata actaccatcg
     2221 tcaacttatc tggattggca ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat
     2281 actcggtgga tgcagacatg atcttcataa aagtgaactg atcgactaat ttgaagatcg
     2341 ttacccctga acccacaata ataaacggaa tcgcttcttt aacggtatct aacaacaatt
     2401 ccttcgttgc aatatttatt ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt
     2461 gatgtttctt caaaaaatac aaaaggacaa gaatacttgc tagcataccg ataaatgccg
     2521 cgaaagttga ttgcgtgacc gccgtcacat agtcaccatc catgactttc atgatgatga
     2581 atgtagccaa tagcatataa aaaatacgtg ccacctgttc tacaatctgc gacagcgcat
     2641 aaggcatcat ttcttgattg ccttgcaaat accctcgtat cacactcata caaggaaaaa
     2701 ccaatacagc gatactcaaa gaacgcatcg ttggaatcag ttcttctccg ccaccagaag
     2761 catgagctag ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc
     2821 ccatcatctg taatgcacgg ataaataatc gtcgtgaagt gccatattca ttcaatgaat
     2881 tatagcgtgc agtttgtttt gcaatcgcag caggtattcc tgctgtagag atcagtagaa
     2941 acaatgcata tatattgtat cccatattga ataatccatt ggcagcctta gcattttcgc
     3001 ccatccaagc ataccaggga atgatataga cagcgcctag caatcttgaa atgatattac
     3061 tggcggtcat ccaagcagag cctcgtgcca ttttttcttg cggtgttaat tgatctaatg
     3121 ctggttttct ttgattatcc atcggtcaga ttccaacttt ctacaaaaca ttactatcat
     3181 tttaattggt aaaacagaaa ctggcaatcg attttatata acttttataa gaacaattgt
     3241 ttttttgaat tcggtttcgt ttttttggta aagtaaagaa atatacagcc ttttatggct
     3301 aacatttata ggagatatca atgacacaag aaataaaaaa actacccgtt atatttttag
     3361 gtcttttctt tatcacagtt ggattgaatt ggttcttgct ccctcacgat atagcttccg
     3421 ccggagtggg cgccatcgga catttagttg aaacggcctt ttctatccaa cgctcagtta
     3481 ctgtttggag catcaatctt atgatgctgc ttctagccgc tgtcctatta ggaaaatcag
     3541 tattttttaa gactgtagcg ggaagtctgc tttttcctgt tgttttagag attgttccaa
     3601 aagtggaaat gatctcctca catttctttt cactgatttc aggaagtctt ttgttcagtc
     3661 ttggggttta cacattgtac acagtcgggg cctcaaatgg cggtataacg ataccgccga
     3721 ttattttcca caaatttttt cggcttccaa tgccgcaagg acttttactt accaatagtc
     3781 ttatcgtctt tttgaattat tatgtttttg gattgttgga gacactattc gttctcctat
     3841 ctattacagt cagttcattg tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa
     3901 aatttgaata aagctggatt ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg
     3961 ctataataaa ccaagaaatt gaataaagcg agggatcaat caatgagctt ctctttagaa
     4021 gacatccgtc atttactatt aaaagaaaat ctattaaaag aatttgtttc aacaagaggc
     4081 tggcatcttg atgtaccaga aaaagctgaa tttcttcagt taagttatga ttcaagaaaa
     4141 gctgatgctt ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga atatttggat
     4201 tcagcaatcg aacagggtat tcaatattat gtatcagaac aaccatacga aaattcagca
     4261 gtaggaatca tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat ggctttttac
     4321 gactatccgc aaaataaatt gaaagtgatc ggattcactg gaacaaaagg taaaacaact
     4381 gctgcgtact ttacaaaagc aatcttggat cacacaacaa ataaaaaaac agcattgctt
     4441 tctactatga atacaacgtt agatggcaaa acatatttca aatcccattt gacaacgcct
     4501 gagtcattag atttatatcg tatgatgtca gaagcagttg aaaatggtat gactcatcta
     4561 gtgatggaag tttcttcaca agcttacaaa acacaacgcg tatacggctt gactttagat
     4621 gtcggaatct tcttgaatat ctcgcctgac catatcagtc cgatcgaaca tccgacattt
     4681 gatgactatt tctattgcaa gcgacaattg atcttgaatt caaaaacagt tgttttaaat
     4741 catgaaagtg actattttga tttacttagg gaaacagccg atctattcca gattccaacg
     4801 atcacttatg gtcgttcgga agaaagcaat tatcaagtga tccgttcaga taaaggtact
     4861 cacggtttta ctttatcttc tcatgaagat cgcttagcta tctgcgatac tgcttatgat
     4921 attttgctgg ctggaggatt caaccaagaa aatgcagcca gtgcgatcat tgccgcagca
     4981 ctaacaggtg cctccagtca agatgcacaa gacggattaa aagaagctcg tgtaccaggc
     5041 cgaatggatc aattgatcca atccaatgga gcgcatgtct atgtggacta tgcacataat
     5101 tatctaagct tgaaaacatt gctgaaattt gccaaaaatg aacatccaga tggccgtgta
     5161 attgttgtat taggcagccc aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa
     5221 gtgctatcag aaacagctga catcgccttt ttgacggcag atgacccagc atttgaagat
     5281 ccgaaaaaaa tcgcggaaga aatcaatgaa gcaattacta atccagattt gatcgttcat
     5341 tatgaaatgg atcgtcctga agcgattcga cgcgctttag cagaaagcgc tccgcaagat
     5401 tcagtcgtta ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa
     5461 ccttatgaag gggattatgc aatcgtgaag cggttgattg aagaataatg gtatttatat
     5521 actagtggtt tcccatttct aagaaaaaaa gggactgtga ttttttgtca cagttccttt
     5581 tttcttagaa atcattttaa cttttcacta gctaattctg ctgccttttc tctttcttca
     5641 tttaataatt tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt
     5701 gcacaaatga ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg
     5761 atcccgactg gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga
     5821 atggcaaaat aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa
     5881 tagggattgt agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct
     5941 ggtacaagtg aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata
     6001 aagatgatca atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat
     6061 tctcctgcca acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc
     6121 ggggtcaaga aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg
     6181 atcaagaaat aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca
     6241 aatgggatag caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg
     6301 atcatcacca caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact
     6361 cctggcggaa caacatctgg catttttatg acccaatttc tcttgacaca catacaatac
     6421 aattcgaccg caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt
     6481 tcgacaaaag agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca
     6541 atcaagctga ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa
     6601 acagatagta atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt
     6661 gacgttaatt cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt
     6721 gtattgacga tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct
     6781 aaaaatggga acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg
     6841 aaaggaatag aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt
     6901 ttccccatag gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc
     6961 ctaaatataa cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg
     7021 aaattttctt taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa
     7081 tgaaaacaaa taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc
     7141 ctgaggtaaa caacactgga atcactaata aattaaagac actctggaac aaaaaatacc
     7201 actttgtcca tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg
     7261 caggcaaaat agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata
     7321 gaagcagaat catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga
     7381 ttagataacg attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt
     7441 tcctttttac tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat
     7501 aattcatttc ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat
     7561 ggaccatgat aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag
     7621 tttgtgactg atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt
     7681 cttcaaacat ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc
     7741 cgctatggaa aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag
     7801 ccatctgttt tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa
     7861 taggttcagc taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt
     7921 tagtcacgac cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat
     7981 gaatttcttg atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg
     8041 tttcgattcc aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt
     8101 gtcccatatt ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt
     8161 catttatgct cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg
     8221 tataagtctt gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta
     8281 gagaaaatgc tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct
     8341 ttcgtatttt acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt
     8401 tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg
     8461 tttcattgat ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac
     8521 tgactacgcg attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg
     8581 catgtagttt atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat
     8641 aggcccattc atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc
     8701 tttttactgc gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct
     8761 caatgcgaat acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat
     8821 tgaaaagctg gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa
     8881 ctttcgctaa ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag
     8941 ccatgagatg taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta
     9001 cctcaatacg ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt
     9061 gttcgatgcc taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca
     9121 agtgtaaagt cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt
     9181 catcgagaat gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc
     9241 ctaaatattc tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat
     9301 gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt
     9361 ttagtgaata tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac
     9421 gactgatata aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca
     9481 attggttcaa tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag
     9541 catatccagg tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa
     9601 tatgcagttg gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta
     9661 actctttgat ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag
     9721 ctaaacgttg actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt
     9781 tttccttctg tttcattatc atccactcct attccatcat atgttctctt cgatcacctc
     9841 tgctaacttc tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac
     9901 aggctttcca acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg
     9961 gctgatcaga aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga
    10021 aactgcatct acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc
    10081 catcatcgag gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc
    10141 actcctttta tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata
    10201 tttccgtagc attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt
    10261 gatatactag tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt
    10321 tgtatacaag tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca
    10381 ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag
    10441 aagatccaga atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc
    10501 atcacaaatc cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa
    10561 tgaaagtcga acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt
    10621 atcatccatg tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca
    10681 aaccgtgttt ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc
    10741 ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga
    10801 ataaactggc agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg
    10861 tagaacaaac acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt
    10921 ggctgatacc aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc
    10981 gttctttgaa accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc
    11041 ctgcccacat caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc
    11101 caaatgcata gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct
    11161 ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa
    11221 accaacaaaa aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg
    11281 aatctttagg taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc
    11341 ccattttggg atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca
    11401 acatgcccat aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca
    11461 tgacgattgc taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa
    11521 tactcatcaa catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg
    11581 ttgtaattat aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca
    11641 atgaacaaat atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta
    11701 aaataaacgg atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt
    11761 tacttttatt ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc
    11821 gttgtcattt tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt
    11881 ttcttgtatg attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat
    11941 ggttctatca aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct
    12001 tacgaaacct tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca
    12061 tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg
    12121 aaaaacaaga agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt
    12181 atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa
    12241 agttatttta tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct
    12301 ttactgactg aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca
    12361 accgttattc gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt
    12421 ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga
    12481 gttttatttc cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa
    12541 aattcctcga ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt
    12601 agttacagac atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa
    12661 attggtgatt gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt
    12721 cacgagttgg ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca
    12781 aagagccatt aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa
    12841 atcaaagtat tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa
    12901 tgattttcgc agtgtttttg tatatttttt attaactagt attactacaa aggtttattt
    12961 ttcacttctc aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc
    13021 tctactccga tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa
    13081 ttgatgggta ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata
    13141 tatttcgttt ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca
    13201 atataaactg gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg
    13261 ttggattgtt tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa
    13321 agatcgccac ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat
    13381 accccgttgg cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatctatcg
    13441 ccagttttcc atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt
    13501 gtaactcaaa aacagctcct tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca
    13561 cgacaccgcc tggtgtaagt cgattttctt tcgtcaattc taccaccgct gtctgacttc
    13621 tttcgatctc aaactcgatt ggtgtcgcat ccaattcata ccccgttggc gcttgggttt
    13681 ctaccagctg atacactcct ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg
    13741 tggttagacc tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt
    13801 gcaggacttc cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc
    13861 ggtttgcttt cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgatcg
    13921 gtgtcgcatc taattcatac cccgttggcg cttgagtttc taccagctga tatgctcctg
    13981 gtgctaaacc gtcaattgcc agttttccat cttctcctgt agttagaccg gtttgtagcg
    14041 tttctccttc tcgg
//
LOCUS       DAYYNG010000068        13713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000068 DAYYNG010000000
VERSION     DAYYNG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.068"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..282)
                     /locus_tag="ACHBFA_001610"
     CDS             complement(52..282)
                     /locus_tag="ACHBFA_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9395099.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            578..916
                     /locus_tag="ACHBFA_001611"
     CDS             578..916
                     /locus_tag="ACHBFA_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395100.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1121..1696)
                     /locus_tag="ACHBFA_001612"
     CDS             complement(1121..1696)
                     /locus_tag="ACHBFA_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFM9395101.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2062..2643)
                     /locus_tag="ACHBFA_001613"
     CDS             complement(2062..2643)
                     /locus_tag="ACHBFA_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9395102.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDAEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2826..3452)
                     /locus_tag="ACHBFA_001614"
     CDS             complement(2826..3452)
                     /locus_tag="ACHBFA_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9395103.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3474..4802)
                     /locus_tag="ACHBFA_001615"
     CDS             complement(3474..4802)
                     /locus_tag="ACHBFA_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9395104.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERFFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4960..5361)
                     /locus_tag="ACHBFA_001616"
     CDS             complement(4960..5361)
                     /locus_tag="ACHBFA_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9395105.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAEMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5447..5653)
                     /locus_tag="ACHBFA_001617"
     CDS             complement(5447..5653)
                     /locus_tag="ACHBFA_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395106.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5748..7250)
                     /locus_tag="ACHBFA_001618"
     CDS             complement(5748..7250)
                     /locus_tag="ACHBFA_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM9395107.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTNPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTRYAQDLVTKIKKGMGTQDDKPLKGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7268..7723)
                     /gene="argR"
                     /locus_tag="ACHBFA_001619"
     CDS             complement(7268..7723)
                     /gene="argR"
                     /locus_tag="ACHBFA_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9395108.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7821..8438)
                     /locus_tag="ACHBFA_001620"
     CDS             complement(7821..8438)
                     /locus_tag="ACHBFA_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9395109.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYDEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8659..10032
                     /locus_tag="ACHBFA_001621"
     CDS             8659..10032
                     /locus_tag="ACHBFA_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9395110.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEKLQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10107..11219
                     /locus_tag="ACHBFA_001622"
     CDS             10107..11219
                     /locus_tag="ACHBFA_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9395111.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTSHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11291..12928)
                     /locus_tag="ACHBFA_001623"
     CDS             complement(11291..12928)
                     /locus_tag="ACHBFA_001623"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM9395112.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVQGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13096..13704)
                     /locus_tag="ACHBFA_001624"
     CDS             complement(13096..13704)
                     /locus_tag="ACHBFA_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9395113.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPVAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DATYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3708 a         2879 c         2443 g         4683 t
ORIGIN      
        1 caatccatac aatatcacct tttttaggaa tataaattct ttcttctgac actataaaat
       61 ttctctccct tcaggagtta aatcttccca ttcgtctttc tcataatact cggcctcttc
      121 cccaccacta aatggatctt caatttttgg aactaacgta attgttccat catcagaata
      181 cacaacgata tattcttggt tttctgaagg tttctttccg ttatcagaag gtaaagttac
      241 cactacagaa cttccttgta atctagattt tgtaacaagc attatcatca ctcccttaat
      301 aaaagtatac tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa
      361 ttgttttgtt taaagtatat cttattggat ataaatagaa ataaattgta gccagttcat
      421 tttttcttta aataaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc
      481 caaatactat agctaactcc ccgctacctt caatcttgca gataaaacgc tttggaaaaa
      541 aatcgagaaa atcgcaaaga cgacaggcca tcctatcatg cctgattata aacaaaaagc
      601 tggctattcc ttttgctctg acaaatttca attgggaaag ttcacgaaac tagcaggtga
      661 agaagtttca actgttcgga ttaaagaatg cccgattcaa atagaaacat tggttcaacg
      721 aatttcaact agacaagact ttgctattgt agaacgtgag atcgtagcta tttttgtaga
      781 agaagggatt ttacatgatc caactcatat caatgtggat aaatggcaac ctcttatcta
      841 taaattcaga gaatatacaa ctgccaacca gtcgttaact ttgaatttta gattccaaga
      901 attcaaagag tcataatgat aaacatcgtt aggtataaat aaaaaagact cgcagcacga
      961 gtaaaaaaca aatagaagct atacattaga aaggtcgact gtgaccctat agatgcagta
     1021 catgcagctt atgagcattt cttctgttct ctttgttttt gggatctgtg ctgaagtctt
     1081 tttcgttttt tgccgctctt tctttgaaaa atcgattatg ttataccttc tccattacta
     1141 attcaggcat ctgctgagta atgatttttc gcgcaaaaga aatatctgtt acccaaggtt
     1201 cgatttgttc ttcagtcaag acaagcggca ttcggtcatg gattggtgcg actgtgcggt
     1261 taggttcagt agtcattatg attgattctg ccattttttc atcctgatgg ctacgataaa
     1321 aaccgccaat atacactatt ttttccttat ttgaaaacat aaatttgtct ttttcttcat
     1381 tccactcata aaagccactc attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa
     1441 aggtcttttt ttcttctact gtctctgcac gggcattgat catcaattgt ccttttttaa
     1501 atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc tcttttctta
     1561 acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct gcctgctccg
     1621 gttgtttctt ctgggcttct cgataataat tctgaaggag ctctgactct agatcaaaaa
     1681 aataacgacc acacatccta atccctccca accttttttt ctatcataca ctgcaacaag
     1741 aagttcgtac caaaaaaaac gctttcaatt tgtgtagaac taaaaaaatt gtgacaagtg
     1801 acaaactctc cagtgaatgg tattctcctg ccaactcctt ggtcacaagt cacaatattc
     1861 aacaaacatg agaagctatt ttttgaaatt gttccttgtg acccgaagtg aaacggcagg
     1921 cttacgacct ctgggtaaac ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat
     1981 ttcacaaaaa tttgagaagc aattttcgta aatgccttct tatttcttga agctgaccac
     2041 ttctgtcaca acctctaatt attaaagaac cttttgcgaa gttaacaatc tttctttgat
     2101 caattggaaa ttctgtctaa gtagtgatag atcttgtgtg actttcaaca ataagatact
     2161 gccctggatt tcagcaatca acgctttggc ttgacggtct gcctctgcat cggacagttt
     2221 ttcttcagaa agtgccagga tattttgttt cgtatacttg acccattgat tgactaattc
     2281 tagtagcaac agacggtatt cttcatccac tgtactcaac tcaataatca aattacctac
     2341 aggacagcca tgataagtga tctgtgtctc atgatcagcg aaaatccagt ccaacatttc
     2401 ttctaatgct tcttctggct gttttccagc atttaagatg tcttcgaaca attctgtctg
     2461 ccatacgtcc atatgcgcat ggatcaccgc tagacaaact tctttttttg aagaaaagta
     2521 ataataaaat tgaccttttc ctgtatcaga agctgctaga atatctcgga ttgacgtttc
     2581 atgatagcct tttttataaa taagatcttt ggtcgcttcc aagatctttt cctttgtttt
     2641 caatgatatt ctcctttttc atttctcata gtcctatatt atacagtaaa ttcatcattt
     2701 tttcttctta atcgtttaaa aaagactgtg ccaaattatc tgacacagcc ctcattataa
     2761 aactgttctc atatagctag atgttcattg attcaagaac tgcgaggaca aaagcaattc
     2821 ctatcttatg atagttgctc taatacttcc tctgttgtca tcacttgatc tgcaataaag
     2881 ccaaagttcg tcaacgctgc atccattgct tcttgaccag gagcaccaat tgcatctgtc
     2941 gctaccacaa cattgaatcc actctctacc aattcacgca tatgggaatc gctacacaag
     3001 tttccgttca ttccaccgag aattactgtg tcgattccat tttttctcaa ttgaagcgct
     3061 aagtcgttgc tttctggtcc aaagatttta tgaggagagc tgatgatcgt attttcatcc
     3121 aaatatggtt tcaattcttc gatgaaatca gctcctgaac cttcttcaac tgattcatat
     3181 tgacttttcc tagcaaacat ctgctgttca agcatcatgt gttctcctgc accgccaaat
     3241 tgccatttat ggtcatgtgg gtaaaaataa tgaggagaaa taaagagttt gtatcctttt
     3301 tcttttgctt gtttcaccat ttcgatcatg ttttcgatcg tattgtattt ttctaagata
     3361 tcttttgtca aaccaaagcc accgcctgct ggtttcaaga actcgacttg cgggtcagtg
     3421 agaactactg ctgtattttt attattgaac atggtggatt ctccattcgt tatttatttt
     3481 aatacatcat ttaaagcttc actcattgtt ggatgagaat agatttgatc tctcaataac
     3541 gtataaggaa gttttccatt catagctagc gaaatcaagt tgatgacttc atgagaatct
     3601 tccgcataga tacttgcccc tagaatcaag tcagtttcag ggtcgactaa aactttgaat
     3661 acacctttag gatcttctaa tacttgtgct ttagggacac cgcttgtcat atacttgaat
     3721 aatttgtaat tgacgccttg tgctttggct tgtttttctg ttaaacctac attggataaa
     3781 gtcggagtga taaatacact gtaaggcaca gctttccggt tattcgttgt tcgtttattc
     3841 tcgcccttca actgatccat aatgattcga taatcatcta gagagatata agtgaactgc
     3901 aatccgcctt tgacgtctcc aatcgcccaa atgttgtctg catctgtacg aaggtattca
     3961 tccacagcga ttgctccgcg atctgttagt ttaacatctg tattttctaa tccaagtgat
     4021 gctgtacttg gttttcttcc agttgcagcc aaaacttttg acgcttggat tgttacttct
     4081 tggccatctt ctgtaaaggt gacggcagct gttgattctt ggtctgctac ttgatctaca
     4141 gatacaccta gatggaattc gattcccgca tttgtcatgt cttctaaaat catttgcgcg
     4201 atatcttcat cttcacgagg aagaaattca ggatgggcat caagaacgac aactttagat
     4261 ccgtattcat tgaacattga tgcaaattcc aagccaatat atccagcccc gatgatcact
     4321 aatttttccg gtaattgttc ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt
     4381 gtattcaatc ctttgattgg taaaatgatt ggttcagaac cagtattgat gaaaaaccgt
     4441 tcgccttcaa caatcacttt ttcaccatta ttcgtcactt cgatttgatg gttagaaaga
     4501 aaacgtgctg taccgtccat cactgtccca gttgcctcat cgctgatcat atgataattt
     4561 ttggcgcgaa gcatgcccgt caatttttct ttccgagcaa cagcctctgt aaagtcgatt
     4621 cctctttctc cattgaaaat caatgattta gaaggaatac agccgatgtt gatacatgtt
     4681 ccgccataca tacgtgttga cttttcaacg actaaaaccg attcgccttt tgaagctaac
     4741 gccttcgcta aagtcttacc gccctttcca aaaccgatca caatattttg atatttttcc
     4801 atcctttttc cctcgcttct tttttaccta ccgattggta caaaaatatg ataaccgata
     4861 attgtctatt ctccaaatca tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc
     4921 ctttcacgtt tttagtctgg ttcgatttcc tttattcttc taaacaatcg ttacttctac
     4981 atcgatattt cctctagttg ctttggagta agggcagaat tcatgcgcat cttctgcaat
     5041 tttttgggct tcttcttcgc tgatgccatc gatatgcact tctaatacta caccgatctg
     5101 atagccacca tcatctaaat tataaagaga gacacgtgct tttactgtgg actctgctgt
     5161 catctctgct tctttaatca tgtgctgtac tgcaccattg aaacaagaag cataacctgc
     5221 tgcaaataat tgttctggat ttgttccttc tgcatggatt cctggaggag tgattttcat
     5281 atgcatcgag ccatttggtg cctcgacttc tccattgcgc ccgccatggt tgatcattgc
     5341 tgtttcgtat gcttttttca ttgtactgct cctttcgatt gaccatttgc tacttggcta
     5401 taactatagt gtatgacaag taaggcgctt tctacaaaaa ttctgcttat tcgaatacca
     5461 gaatgccgtc ttctactatt tttccaaaaa acaaaaaaat actactcgtg aacaacagcg
     5521 tcatatttgt tccaaaataa atagcaatct tttctacata atcttttata tgattgattt
     5581 taagatacat atataccaga aagttgaccc cataaaccaa tactgttaaa aaaataacca
     5641 tattcgttac cattttttcc tccttttgct ggtctcccta ccagcactcg atttaaccat
     5701 accacttttt ttgccgtcca agcaactagc ctttcaaaaa gactggttta ttctgctgtt
     5761 aatgattcaa gatcagaaaa atgtccgaat aattttgcta attttactaa cgccaacttg
     5821 ttctttccga attcatcatt ttctatctgt aatactagta acggctcatg taagcttaaa
     5881 cggatcaaaa accagccgtt tccgtatggc tcttctagat catatcgaat accttcttca
     5941 ttttccgggt cgaagttcat ccctttttct tcttccaaca gtcgcttcat ttcttctaat
     6001 atcttgtttc cttgttcgcg tggttcatcg ctgaacaatt ttaatcggat ttcttgtact
     6061 tctacgggct gtttcaactc tttgatttgt tcatctaatg tgataccttt tctttttaat
     6121 tctggcaaaa gcatcaggat ctttgcgatg acatatgcac catcatccag aaaatagttt
     6181 tccttgaatg cagcgtgtcc gcttgtttcg atggctagct gtgtatcttt cccaagacgg
     6241 ttaagttcta atgctttatt gattacattt cgatagccgg aaatataacg aacttggatg
     6301 cctccttttt cttcaatgaa tctttgtaga tgagaagaag taggggaatt cgtcacgatt
     6361 cttgttcctg gatgctcttt taatacgatt gtacttaata ctgcgatcaa attatttcga
     6421 ttgatcactg ttccggaagc actgacaact gcagaacgat ccacatctgt atcgaaaatg
     6481 atcccaagat ctgcttcatt tgctaaaaca gcctgcttga tactttccat cgcttcttta
     6541 ttatcaggat ttgggatatg attaggaaat cggccatctg gttccaaaaa ttgggaacct
     6601 gttgaatccg cacctaagct attcaatact ttttcagcaa agaatccacc agcaccattt
     6661 ccagcatcca cgatgatttt ccaacctttt agtggtttgt catcttgtgt tcccatccct
     6721 tttttgattt ttgtgactaa atcttgcgca taacgtgtca aaaggtctgc tttttctacg
     6781 cttccagttt gttcgctttt tattttttgc tgctgtgata ggataaaagc aatatcttct
     6841 ttttctgctc ctccttctga ggtaaagaat tttattccat tataataata gggaagatgg
     6901 ctagcagtca gcataaccgc agcatcgcac gagaactgtt caaattgagt ggacataaac
     6961 atagcaggcg tagtagctaa ttcgaaatcg atgacatgta tcccttgttc tttcagactt
     7021 tggatcaacg cctctttcag tgctggtccg cttatcctac tgtcatgacc aatcccaatc
     7081 gtccattttt tatttggatt ggttgacttc ttctttttct ttatccagtt tgctagtccc
     7141 cagccgatct tttttaccat gtctgtcgtc aagtttactt catatccttc aactggtaat
     7201 gcgattcctc gaatatccga tccattttgt agcgctttca tatctttcat ataaaagtcc
     7261 tcctcattta ttccctcatt ttttccagct tttctgcaaa tcgctgtgct tcttcttctg
     7321 agcggcaaat aataatgaat gtatctacac cggccatcgt tccgacaact tcagggtaag
     7381 ccacttcatc tagccaattc gttaccacat cggcaccatt tcgttcagtc aatacgatga
     7441 ccatgaactg aacagactgg atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt
     7501 cttcatttaa agaaacagat ggctggttaa ataatgtata gcgtactatt ttgttttcat
     7561 tttgactttt tactaacttc atttctcgaa tgtctcgaga aatcgttgct tgagtcacat
     7621 cggcgcctgc ttcatgcagc cgcgcaagca gctcttcctg tgtcccaata ggttgttctg
     7681 taatgatctg gcggatcagt gcttgtcgct gtttcttttt catattagac acctctcaat
     7741 gttgattata gcaaaagata agtaatattt cttcatattt tggtattttt tacttttatt
     7801 ttttgttttc attcaggttg ctaacctttt tctattttaa cacgcaaaat cgtcttcatt
     7861 ttttcaggtg ctgattttct tggatcgctg atgtaaatct ctttgtgttc tttagaagct
     7921 ctttcatagc cttcttttgc gataaatgct tctaatttct caaacgttgc tggttcttcg
     7981 tcatagggcc cgacgtgtaa gatttgggca caaagacctt cttccatctt tttgaatact
     8041 acttgttctt tcaagtcttc cggaagtttg ctagaacgct gaatggcttc ttgtgctatc
     8101 gcttctgtaa caaagtcagg ctgtcggatc atcaattgat aagaaaaatg ttcttttttc
     8161 atcactggtt catgcaaata cttttcttgc agtccccatt gtccttccag tggatagaca
     8221 gtatacatct gataatcagg aatcagccaa ttttttttag gcgacatgcg tattgcatag
     8281 ctgactgcat atagacaggc tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc
     8341 cctctgatgc taaagaatgg ttttttagga acctctagta tttgcgggct ttttttcata
     8401 ctatagctct tgtctttacg ccattcaatt ttttccattt ttcttcctcc gcgaaattcc
     8461 atttagttat ttattatcat acctcttttt gagtgacttg gctaatcgtg ttgacactat
     8521 ttttcagttt gtgctagaat aagttaagta aataaatcgt acagataaca cgtttcggcg
     8581 tgtcgagaga gaggcggttt ctatttttat aatagaaatc actctctttt tttacgaaat
     8641 tttttagagg tgatgtaaat gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt
     8701 gcaacaggga tgatgtcttt tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc
     8761 ccaactctta tcagacagtt tgatttaacg actgcacaag tccaatgggt aacaacgatt
     8821 tatcttttga tgatttcgat cattgttccg ctgtctactt atttgaatcg caattttagt
     8881 ctcaaaaaac tctttttagc ttcaaatctg cttttcttgg caggtataat gatcgatttc
     8941 ttctctccta ccttcatggt cctattgttt ggtcgattgc tccaaggtgc agcgacaggt
     9001 atcgctcttc cgttgatgtt ccatattatc ttgacatttg cccccctcaa tcgacgaggc
     9061 gcgatgatgg gtgtcggtac tttgacaacg gccattgctc cagcaattgg tcccacttat
     9121 ggaggcatta tgacttcaca ttttacatgg aatcatatct tcctgtttct tgtgcccgtt
     9181 ttgattcttt ctttgattat cggctggttt gctattcccg agatggcagt acaaaaaagt
     9241 ggttatttgg attggataag cgttttagga atttgtcttt tgtttagtgg aagtctaaca
     9301 tttttgagta cgttgggaac agtttccagc tggattgctt taatcatcgg cggtattggc
     9361 tggttccttt tttactatcg aagtaaaaaa gtggagcatc cgctgatccg actgtctatt
     9421 ctttcaaata aaacattccg cttgtttttg attggcttct tagtttatca gtttttatta
     9481 cttggtgtat cttttgtctt gccgaatttc cttcagatcg ttcaaggaag tagtcctttt
     9541 gtcgctggat tagcgatgct tcccggcgca gcggttggtg ctttgctttc tcctttttcc
     9601 gggaaaatgc tggatcaata tggtccgaag aagccaattt ttgctgggct gattctttct
     9661 ttgattggtt ggctagcatt attcctactc ataggacacg cttcagtggg tgtattgatt
     9721 gcttgtcatg tgttttatat gatcggtatc ggattatctt acagcaatat gatgaccacc
     9781 ggaatgaatg cactgaatga aaaactacaa ggagacggga acgcaatgtt caataccttg
     9841 cagcagtttt ctggcgctgt ggctacttca ttagtagcgg tcattatcaa ttacgtccag
     9901 catcatactt cccataacta tgaagtgtct actacattag gatcaaaagc agcattaggc
     9961 gtcttgttgc tgttgcttct cgtaagcttt gctcgattcg cctattatct gttctttgct
    10021 aaaaaagcgt agattccata tacggtaggt gttgcatctg ccgttttttt gtgtgaaaat
    10081 ggttgtttgt aaaaggaggc ttttttatgg aaaaaattgc cattatcggt ggcgggatca
    10141 ttggtatgac cctagccagt tacttagata catcaaaatt caatgttgct ttatttgatg
    10201 aagggacagg acaggcaact aaagctagcg ctgggattat ctccccttgg ttgtcgaaac
    10261 gcagaaacaa acaatggtat cgtctggcta aagacggcgc tgcttttttc cctaaactta
    10321 taaaagattt ccaacttgat caaacgattt acgcacaatc agggacactt ttgcttcgac
    10381 ctgaaaaaga tttagctgaa ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag
    10441 aaattggaaa catcgaatgg ctttcacctg aacaaacatc tcaagcactt ccgttattga
    10501 aaccagcagt ttcactgcgt atcagtggtg gtggtcgtct ggatggaaaa gcatatttag
    10561 aacatcttgg tcagcgttta gcacaaaaac aaatggagca gatccaggca aaagtcactt
    10621 cgcataagaa gagcgattct tggctcatca agggaccgtt tggagaatac acagctgatc
    10681 gtgtaatttt gacacctggc ccggcactta agcctttatt acaagaatta ggctatgctg
    10741 cagatatacg tccgcaaaaa gggcagctct tagcatttca gacatcttat aagatgagtc
    10801 aagactggcc tgttgctatg cttgacggag aagctgattt cattcctttc atggatggaa
    10861 agatcctttt aggtgctacc catgaaaacg atcaaggctg ggatttggct cctactagcg
    10921 aagcttataa gggattgact aagagtacag aacagttctt gactgatcca gatcagataa
    10981 aaaactggcc atacgagttc cgtgtaggta cccgtgctta tacgtcagat ttttccccat
    11041 ttttcggcgt actgccagat gatccaagct tactggctgc tagcggtcta ggctcttctg
    11101 gcttgaccac aggtccttat atcggctatc tattagccaa cttcttaaat actggtcatt
    11161 ggcctacgga agattatcaa aaggacatct ccacctatat tacgaaaaat ccatcataga
    11221 aaaacagctt tttcaagtaa ggatcccgag cgatattgcg catggattac ttcttgaaaa
    11281 agctgttatt ttagttcaat ttctgaatca aattctccaa actttctttt gcttcatctg
    11341 gcaagagatc ttttcctatc tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt
    11401 tcaccagatt tttataggac gtatcctcaa aagaaacagg aaaatctggt ataggtaaat
    11461 agtggatgtc ttcaaatgga ccaaacgact tgaattctgc cgtttgttgc acgattcttt
    11521 caacgattga caatgtcgtt tcaggtttga tttttttatc atcgaaatat ccagtattca
    11581 ggacatagca ggctgtttgc tgtttcaaaa acaactctcg aaaagcttga tagtcttccc
    11641 taagaggata gacacggaaa ggattagcaa atggttcaat gactagctca tctagctgtc
    11701 tttttgtagt gatattttcc gcgtctgatc gttttgttgc taatgttgca ccaaaagacg
    11761 ctgctaaaac cgggtcattg atcttgataa ttggcggaag cgaatcatct ttcatgatcc
    11821 aaaaaacagc atcgatcttt tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg
    11881 atttgactgt ccgcccattt ccgtttcgta tatctcctgt tactaggact ttttctcctt
    11941 tacttgtaca tgtcacgccc acattctgac aagtaaggaa agaagaaatg gccggatcat
    12001 cagacggata gtcttgtgtt ttatcaaagt aagaaggttc caaggcagta gcaaaaccat
    12061 tttttcgaga aataacgaaa gcatcgtcat gcaagatcga aacagagtga ccttccgtat
    12121 gttttgctaa ggtaatcgtt gattttccag aaccggacaa cccaaataca gccatcgtat
    12181 aggtctggtc attactagca tattgtttca tcccaccgtg gcaggcaata aaaccattgc
    12241 gatttcccaa tgcccaagca agcgtcaaag tcgctttttt taattctcca aagtaacgta
    12301 atccttgcac agccgctgta ttatggagag catcaaacaa aatgagtcca ttgggaaatg
    12361 tttcatgtgt ccactcggga tcacaaaaga tataaagatc tccctcatca aaggaaacag
    12421 acttttgata agtctttaag atcttcgaat tcgctggctg aaaattcagc aaataagaat
    12481 acaaattgtt ctcaaatcct tctggaatca gcaaatgact ttgcacagaa aattcttcag
    12541 ataggccaac gattactttt gtatggtaaa actggcgttt gcgactttga tacacagctt
    12601 ctcttaaaat tttctcgtat ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag
    12661 cacttgctgt tcttcccaca atgcttccat cattgaatac tagtgtcctg acatcagctg
    12721 gtaaatctag ttcttcagca tgtgagattg ttaaatctgt gatgatcgtt cctggactcc
    12781 cttttgctag ttgataagct gtcttcctgt caaaaacttc atgaacatta ttaccgtaaa
    12841 atgtcgtttc tacaatcgat cgaatactgg ataacagtgg atttccactt gtgatttcgt
    12901 ttaattcgag actctctttt gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt
    12961 tttctttatt atacattatt cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg
    13021 agttattttt cacacaaaaa aaccgagaac ttgaataaac aagtttctcg gtttcatcat
    13081 tttcctttta tcggcttagt accagccgtt tgctaaccag aatgttttag cagcatccca
    13141 tgaaccataa cgagatgtta catattgttc tgctacacgt tcttgattgg cagctgaata
    13201 gtcgccgttt aagtatgtag catctaattg ataacggccg atgtaacgtc cgttagtagc
    13261 tgtgtatgaa ccgcctgatt ctttttgtgc gatccattct tttgcagagc ttgaagttgg
    13321 cgctgttgca gcaactgggg cagcaggagc tacttcaact gtttcaacag gtgcaacgta
    13381 tgtatgttct tgcgttgctg tttcagttgt agatgctgta ttttctgttg ttttgttttc
    13441 tgttgttgct gaagaatcat ttgaagttgg gattgttaat tgttcaccaa cataaatgcg
    13501 gttgatgttt gcgatcgaat ttttttcagc aattgcatcg atcattgagt tgtctcctgc
    13561 aaatttttgt gagatcttag ataatgaatc acctgatttt actgtataaa cttcatcagc
    13621 gtgagcattt gtagttccta caaacattgc agtagcacca gcagcaaaag ttgtacctaa
    13681 aacaattttc ttaaatgaat tcatattaaa ctc
//
LOCUS       DAYYNG010000069        13638 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000069 DAYYNG010000000
VERSION     DAYYNG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.069"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="ACHBFA_001625"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="ACHBFA_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM9395114.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="ACHBFA_001626"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="ACHBFA_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9395115.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="ACHBFA_001627"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="ACHBFA_001627"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9395116.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="ACHBFA_001628"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="ACHBFA_001628"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9395117.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /gene="rpsF"
                     /locus_tag="ACHBFA_001629"
     CDS             6088..6387
                     /gene="rpsF"
                     /locus_tag="ACHBFA_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9395118.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="ACHBFA_001630"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="ACHBFA_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9395119.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /gene="rpsR"
                     /locus_tag="ACHBFA_001631"
     CDS             6975..7211
                     /gene="rpsR"
                     /locus_tag="ACHBFA_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9395120.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="ACHBFA_001632"
     CDS             7354..9327
                     /locus_tag="ACHBFA_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9395121.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="ACHBFA_001633"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="ACHBFA_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9395122.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="ACHBFA_001634"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="ACHBFA_001634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9395123.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12206..13498
                     /locus_tag="ACHBFA_001635"
     CDS             12206..13498
                     /locus_tag="ACHBFA_001635"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9395124.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4545 a         2467 c         3001 g         3625 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
     4441 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
     4501 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
     5881 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    12001 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    12061 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    12121 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    12181 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    12241 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    12301 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    12361 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    12421 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    12481 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    12541 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    12601 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    12661 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    12721 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    12781 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    12841 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    12901 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    12961 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    13021 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    13081 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    13141 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    13201 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    13261 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    13321 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    13381 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    13441 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    13501 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    13561 cgataataat aacttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc
    13621 acagtccctt tttccgaa
//
LOCUS       DAYYNG010000070        13428 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000070 DAYYNG010000000
VERSION     DAYYNG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.070"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHBFA_001636"
     CDS             complement(<1..914)
                     /locus_tag="ACHBFA_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM9395125.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1341..1904
                     /gene="efp"
                     /locus_tag="ACHBFA_001637"
     CDS             1341..1904
                     /gene="efp"
                     /locus_tag="ACHBFA_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9395126.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2005..2175
                     /locus_tag="ACHBFA_001638"
     CDS             2005..2175
                     /locus_tag="ACHBFA_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395127.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2390..3649
                     /gene="lysA"
                     /locus_tag="ACHBFA_001639"
     CDS             2390..3649
                     /gene="lysA"
                     /locus_tag="ACHBFA_001639"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9395128.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3636..4436
                     /gene="dapF"
                     /locus_tag="ACHBFA_001640"
     CDS             3636..4436
                     /gene="dapF"
                     /locus_tag="ACHBFA_001640"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM9395129.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4439..5320
                     /gene="dapA"
                     /locus_tag="ACHBFA_001641"
     CDS             4439..5320
                     /gene="dapA"
                     /locus_tag="ACHBFA_001641"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9395130.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5317..6033
                     /gene="dapB"
                     /locus_tag="ACHBFA_001642"
     CDS             5317..6033
                     /gene="dapB"
                     /locus_tag="ACHBFA_001642"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9395131.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6039..6734
                     /gene="dapD"
                     /locus_tag="ACHBFA_001643"
     CDS             6039..6734
                     /gene="dapD"
                     /locus_tag="ACHBFA_001643"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9395132.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6750..7751
                     /locus_tag="ACHBFA_001644"
     CDS             6750..7751
                     /locus_tag="ACHBFA_001644"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9395133.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7748..9109
                     /locus_tag="ACHBFA_001645"
     CDS             7748..9109
                     /locus_tag="ACHBFA_001645"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9395134.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9069..10220
                     /locus_tag="ACHBFA_001646"
     CDS             9069..10220
                     /locus_tag="ACHBFA_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9395135.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEIIQSI"
     gene            10195..11364
                     /locus_tag="ACHBFA_001647"
     CDS             10195..11364
                     /locus_tag="ACHBFA_001647"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9395136.1"
                     /translation="MMKLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11539..12918
                     /locus_tag="ACHBFA_001648"
     CDS             11539..12918
                     /locus_tag="ACHBFA_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9395137.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13199..13428
                     /locus_tag="ACHBFA_001649"
                     /pseudo
     CDS             13199..13428
                     /locus_tag="ACHBFA_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3680 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaat aaaagtcaaa ggcttgaaag aaaaaaatat
     1201 ttcgatagca tcgtttaccc gtgtttattt ttctgacaaa taggcatgag accttaaaag
     1261 atagagaata ctctagtttt ctggccggtt gtctgataca ataaagttga ttatgataaa
     1321 gaaaagaagg agcaaaaata atgatctcag caagtgattt aagagccggt atgactttcg
     1381 tacaagacgg caaactgatt aaagttctgg atgcaagcca ccataaacct ggtaaaggaa
     1441 atacagttat gcgtatgaaa ctgaaagatg tccgttcagg tgcaacatac gacacaacgt
     1501 tcagaccaga agaaaaattt gaaaaagcac atatcgacac aaaacctgta caatatttgt
     1561 acacgatgga tgatacagct ttcttcatgg atttggaaac atatgagcaa tatgaaattc
     1621 ctgtagaaac agtagaagaa gaaatgaaat atatcctaga aaacatggaa gtaaaaatcc
     1681 aatttttcgg tagcgaagtc atcggcgttc aattaccaac aactgtcgta ttacgtgttg
     1741 tggaaacaca accttccatc aaaggtgcaa cagtcactgg ttcaggtaag ccagcgacaa
     1801 tggaaactgg cttagtagtc aatgttcctg acttcatcga aacagatgaa ttgttggaaa
     1861 tcaatacagc agaaggttct tatgtaaaac gtgcgtcaaa ataattcgct aacggattga
     1921 taagaatttg aaaacagagc ttgacctctt gaaaggcaga tcatcggatc tgcctttcaa
     1981 gaggtttttt gtggaatcag caaattgaaa aataaaaaag tatttgattt tatttatatt
     2041 ttgaaagatg aatggcaaaa gctagagaaa gaagaaactg cacttattta tgaagaaatt
     2101 gaaatgattc ataaaattga aaatatccga actattccga actatattga agcgaaaata
     2161 ttttttcatc actaaatagt gttgaattta tgtttttatg tataaatagc ttctaatagt
     2221 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
     2281 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
     2341 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
     2401 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
     2461 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
     2521 acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca
     2581 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
     2641 tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga
     2701 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
     2761 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
     2821 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
     2881 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
     2941 aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
     3001 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
     3061 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat
     3121 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
     3181 aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg
     3241 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     3301 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     3361 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     3421 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     3481 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     3541 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     3601 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     3661 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     3721 cttctttatg cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc
     3781 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     3841 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     3901 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     3961 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     4021 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     4081 taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     4141 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     4201 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     4261 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     4321 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     4381 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     4441 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     4501 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt
     4561 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     4621 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     4681 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     4741 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     4801 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     4861 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     4921 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     4981 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     5041 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     5101 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     5161 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     5221 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     5281 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     5341 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     5401 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     5461 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa
     5521 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     5581 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     5641 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     5701 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     5761 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     5821 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     5881 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     5941 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     6001 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     6061 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     6121 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     6181 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     6241 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     6301 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     6361 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     6421 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     6481 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     6541 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg
     6601 cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg
     6661 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     6721 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     6781 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     6841 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     6901 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     6961 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     7021 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     7081 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     7141 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     7201 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     7261 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     7321 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     7381 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     7441 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     7501 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     7561 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     7621 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     7681 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     7741 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     7801 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     7861 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     7921 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     7981 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     8041 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
     8101 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
     8161 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
     8221 cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc
     8281 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
     8341 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
     8401 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
     8461 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
     8521 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
     8581 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
     8641 tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc
     8701 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
     8761 tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga
     8821 attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc
     8881 tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt
     8941 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
     9001 catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt
     9061 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc
     9121 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc
     9181 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
     9241 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
     9301 ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
     9361 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
     9421 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
     9481 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
     9541 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
     9601 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
     9661 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
     9721 taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta
     9781 ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
     9841 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg
     9901 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
     9961 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
    10021 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
    10081 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
    10141 cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg
    10201 aaattatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa
    10261 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
    10321 tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct
    10381 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa
    10441 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
    10501 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
    10561 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
    10621 atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
    10681 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
    10741 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
    10801 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
    10861 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
    10921 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
    10981 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta
    11041 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
    11101 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
    11161 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
    11221 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
    11281 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc
    11341 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
    11401 gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct
    11461 tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata
    11521 ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga
    11581 gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta
    11641 tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac
    11701 aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat
    11761 gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg
    11821 gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt
    11881 tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg
    11941 atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa
    12001 aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa
    12061 gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat
    12121 gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca
    12181 aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa
    12241 aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg
    12301 ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa
    12361 acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca
    12421 cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta
    12481 aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca
    12541 ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa
    12601 gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca
    12661 gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa
    12721 ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca
    12781 ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag
    12841 ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa
    12901 gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt
    12961 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga
    13021 cttattaccg cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca
    13081 cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat
    13141 tttttatggt taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg
    13201 gcaaaggcga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat
    13261 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
    13321 tttctctatc aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac
    13381 ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAYYNG010000071        13159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000071 DAYYNG010000000
VERSION     DAYYNG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.071"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..2123)
                     /gene="glyS"
                     /locus_tag="ACHBFA_001650"
     CDS             complement(48..2123)
                     /gene="glyS"
                     /locus_tag="ACHBFA_001650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9395138.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2125..3042)
                     /gene="glyQ"
                     /locus_tag="ACHBFA_001651"
     CDS             complement(2125..3042)
                     /gene="glyQ"
                     /locus_tag="ACHBFA_001651"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9395139.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3428..4246)
                     /gene="recO"
                     /locus_tag="ACHBFA_001652"
     CDS             complement(3428..4246)
                     /gene="recO"
                     /locus_tag="ACHBFA_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9395140.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4389..5288)
                     /gene="era"
                     /locus_tag="ACHBFA_001653"
     CDS             complement(4389..5288)
                     /gene="era"
                     /locus_tag="ACHBFA_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9395141.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5303..5698)
                     /locus_tag="ACHBFA_001654"
     CDS             complement(5303..5698)
                     /locus_tag="ACHBFA_001654"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9395142.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5682..6158)
                     /gene="ybeY"
                     /locus_tag="ACHBFA_001655"
     CDS             complement(5682..6158)
                     /gene="ybeY"
                     /locus_tag="ACHBFA_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9395143.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6187..8379)
                     /locus_tag="ACHBFA_001656"
     CDS             complement(6187..8379)
                     /locus_tag="ACHBFA_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM9395144.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8403..9374)
                     /locus_tag="ACHBFA_001657"
     CDS             complement(8403..9374)
                     /locus_tag="ACHBFA_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9395145.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10079..11458
                     /gene="brnQ"
                     /locus_tag="ACHBFA_001658"
     CDS             10079..11458
                     /gene="brnQ"
                     /locus_tag="ACHBFA_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9395146.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11740..13080
                     /locus_tag="ACHBFA_001659"
     CDS             11740..13080
                     /locus_tag="ACHBFA_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9395147.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3615 a         2825 c         2433 g         4286 t
ORIGIN      
        1 gagacacagc acgtttcgtg catgctctct gtttttcaaa caaaagacta ttttgtgatg
       61 atttgatcca agcttgctaa tgacaaagcc atggcagcta tttgacttag ttgtgttagt
      121 cggttcttac gtactgctgg ttcatcagcc atgaccatcg tttgatcaaa ataattttcg
      181 atcagtggac gaagagcgac aagctggtcg tagttttcgt ggatcgtttg gtcagcaaaa
      241 cttgctttca ttgcttctac tgctttgtgt aattctttct ctgcgtcgtt ttcgaacagc
      301 tcagggtcta cttctgattg tttttcaagc tgcgcttttt tcgctaagtt cacgacacgt
      361 gtcaaagctt ccattgaagg tttgaagtct tcatctgcaa gatgttgttt gaacaattgt
      421 gctgctgcaa atacttttgt cagatctttt tgttccgcat tcaaaacagc atcgatgaca
      481 tcatgacgaa tattcttcgt tgtcaataat tggcgcagac ggcctttgat aaagtcgatc
      541 acttgttgtt gcccttctga taaaccaatg ccgaaacgat ccgtatcttt actgataaca
      601 tcgctaatcg tttcttgcag aacagacaaa ggaaaggccc attttttcga ttcgatgatc
      661 cgaacgacac cgtatgcttg gcgacgtaag gcataaggat cgtttgaccc gcttggaatc
      721 atcccaactg caaagaaaga aaagatgcta tctaatttat ctgcaatcgc taaaacagaa
      781 cctactgtag actgaggtag ctctccttca ctggatatag gcatgtaatg ctcacgaatc
      841 gctgtcgcta cagcaggttt ctcgcctttc agaagagcat atttttcgcc catgatccct
      901 tgtagttcag gaaattcccc taccatgttg gttactagat caaatttata gatttcagca
      961 gctcgttgca aatccatcaa ttcttcctca gatagtccta cttgttcgcc aatgatacgg
     1021 gcaatgactt gtacccgttg cattttctca tagatactac cgattttttc atggaaagta
     1081 acagttttta gtttttcaac gtattggtcg attgaagctt tcttgtcttc attataaaag
     1141 aattccgcat cttctaaacg agccgtcaat actttttgat tgcctcgaac cacatcttcc
     1201 aattttaccc gatcaccatt acgagcagaa ataaaatgag gaagcagcat accttgatca
     1261 ttccgtacct cgaaataacg ttgatgttct ttcatagaag tgactagtac ttcttcagga
     1321 acagaaagat atttttcttc gaagtcgcca gaaaagacag ttggatattc gactaaattg
     1381 ttgacttctt ctagtaaatc ttcgtctaga tcaataaccc agtgattttt ttctgctaat
     1441 tcgtttgctt gtgtaacgat catttcttgt cttttttgcg gagacgcaat gacgaattgt
     1501 tcattcaatt tttcttcata ttcttttgca tgcgcaaatg tcacatcttc gcctaagaaa
     1561 cgatgacctc gagaggctct gcctgttttg acatccaaga tttcgaatgg gattacttcg
     1621 tcatctaaca atgccacgat ccagtgaata gggcggatat attcaaaatc gtattctgcc
     1681 caatgcatcg ttacagggaa agtcaaactt ttgatcaccc ctgctaagcc tgtcaatact
     1741 tcttttgctt ccttaccatg agtgtatttc gttacataga catattccac accttttaat
     1801 tctctaaaag tgatagcgtc tgtcgtcaga ccttgtccgc ggacaaatcc ttccgctgct
     1861 ttcgtccagt ttccatctgc gtccaaagca atttttttgg ctggtccttt gacttcttct
     1921 tcactgtccg cttgtcgttc tggaatattt gttactttga ttgctaaacg gcgcggtgtg
     1981 gaaaatgttt cgatcgtatc ataagtcaat tggttttcat ctaaaaattt tgctgttttt
     2041 tcttctaatt gtttcatgct tggtgtgaca acatgggcag gcatttcttc cagcccgatt
     2101 tcaagtaata agtcttttgc catcttattc agcctccttc acggtttctg gatttgctgt
     2161 gtttttattc aacaaaggga atcctaattt ttcgcgttct gccacaaaga ttttggctac
     2221 agcacgagcc atgttgcgga tgcgagaaag ataaccggca cgctctgtta cggatactgc
     2281 accgcgggcg tctaataagt tgaatgtatg gctacatttc aagatataat catacgccgg
     2341 atgaacaaga ttttcatcga tacagcgttt agcttctttt tcaaaacgat caaagttgct
     2401 tagtagcaag tcttggtcgc ttacttcaaa agaatatttg gaatgttcat attctggttg
     2461 tttgaagatc tcgccatatt tcacgccatc agcccattct aaatcataga cactttcaac
     2521 ttcttggata taagaagcaa gacgttctaa tccataagtg atctcagaag ttactggttt
     2581 acaaggcagt ccaccaactt gctggaagta cgtgaattgt gtgatttcca ttccgtcaag
     2641 ccatacttcc caacccacac cggcacagcc catggatggg ttctcccagt tgtcttcaac
     2701 aaaacggatg tcgtgttcta atggatcgat tcctaaaagg cgcaagcttt ctaaataaag
     2761 ctcttgaata ttctcaggtg aaggtttcat caccacttgg aattgatgat gctgatacaa
     2821 acggtttggg ttttcaccat aacgtccatc agctggtcgg cgagatggct ctacgtaagc
     2881 agcattccat ggttctggtc cgattgcccg taaaaaggta tatgggctca tcgtacctgc
     2941 tcctttttct gtgtcataag cttgcatcag catgcagccg tttgatgacc aaaatttttg
     3001 taatgttaaa atcatatctt ggacggtcaa ttttttactc atctgttttg cccctttcaa
     3061 ttttaaagcg ttgctgtcaa ataagaatac aggaaagaaa acgacaaaaa atccctatgt
     3121 ctgattacag acatagggac gaataattcg cggttccacc ctacttccga ataaagattc
     3181 ggcagcttca tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg tccggctccc
     3241 actctcccga actcgctaaa ccacgtgttt tttactgatc ttggtcaatg tacgtattca
     3301 ttttgcagtt ttcttgtttc tataatagat cctatcactg aacgatctat ttaatctatg
     3361 gttaataata gacggaactc cctgtgtttg tcaaatggtt ttgatttttt gttgtatgtt
     3421 ttatttacta ttcttctttt tcaggtgtct ctttttcttt ttcagacatg tctttctctg
     3481 acgtctttaa tacgtgtgcc caagacttca tttgattgat gaactttttg cttttcaaat
     3541 ggataccgac atattcatca tacagctggt cgatgagttc acggattgct tgtttggttt
     3601 cttctttcag atggattcca ctaattttat cataagaaat agctgaaaac atccgaacga
     3661 aataaacagc tcgaggatct gcatgataac ggtgatcgtc catcgaccaa tgtctttcgc
     3721 atatcacacc accatatttg gaagaatagt caaattttcc ttttgtttcc ccgcatacga
     3781 cgcagtgtgt ccagattggt gcaattccga agcgttgcag tatttggact tcaaaaatat
     3841 tcgtgatgat ttctgcatcg tctccttggt ccatccgttg cagtgcttgt tgcgtaaact
     3901 ggaacaaatg cgggtcgtac tgatgatctt cgattgccgc atctactaga ttcaataaat
     3961 atgtgccata gccgctcaaa aagatgtctt cttggatgtg tcgaaacgca tggacatctt
     4021 tgacactgct taaaaatgac aaaccttcac ttcgaaaatt cccgatataa actgcttcag
     4081 taaaagggag caaagccgga gctaagggct gattttgccg atggatctct ttgatataaa
     4141 acatcatttt gccagaagat tctgtaaaga ttttgactaa tttatctttt tctttgtaat
     4201 tacggctaga taaaatcaag ccttttgatt ctgcaagctg gctcatattc tccctcctaa
     4261 aaaataaatc tggaaaagca gattctgccg atccagatca tttatgttgc gatcttccgc
     4321 agcttttctc aacacgcgga tttttattca gtttatttct atacctaatt tacaagaagc
     4381 tcagaatatc agtattcgtc ttttcggtag ccgaagtctt gcagatagac ttttttgtct
     4441 cgccagtctt tttgaacttt gacccaaagt tctagaaaga ctttgtctcc taaaagattt
     4501 tcgatatctt ttcttgcttt cgttcctact tgcttcagca tttttccgcc tttgccgata
     4561 atgatccctt tttggctatc tctttcgaca ataatcgtag cctggatatg gattttatca
     4621 ttctcgtttc gcttcatcga atcaacaacg actgcgacag aatgaggcac ttcgtctcgt
     4681 gttaaaaaca agactttctc acggatcaat tcagaaacga tgaagtattc aggatgatca
     4741 gtcacttgat catctggaaa atattgtggg ccctctggca tttcactgac taatacatcc
     4801 atcaaacgtt cgacgttgtt tccttctgta gcagagattg gaatcacttg tgcaaaaggc
     4861 atctgcgttg aataatcttc gatgattccc aacaactcgt ctggatggat tttgtcgatt
     4921 ttattgataa tcagataaac aggagaatgc acgtttttta gacgttcaat aataaaatcg
     4981 tctccccgac cgcgtttttg atcagcacta atcataaata agaccgcgtc cacttctttc
     5041 aacgcactgt aagctgcttc taccataaaa tctcctaaac gatgctttgg tttatggatt
     5101 cctggtgtat caataaaaat caactgagct tctggtgtag tgtatactcc ttggatttta
     5161 tttctagttg tctgtgcctt gtcactcatg atggcaattt tttgacccac gattcgattc
     5221 aataatgtgg atttccctac atttgggcgg ccgacgattg cgacaaaccc tgatttatgt
     5281 tctggcatat atgttctcct ttttaattaa acaacaaatg atatatttta gggacaaata
     5341 aaatcaaccc aacgatgacg gtaaaacaag cggtcactaa aactgctcca gctgccatat
     5401 ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc acgacatttt
     5461 caaaaacagt attgatgatc tctactatcc atacgagaaa aacagctaaa agcagccata
     5521 accattctat ccgatcaagt tggaaaatca cgcccataaa caaagcgagg attccgaacg
     5581 caacatgttt cctcatgttg cgttcttcct caaaaacagt tttgactcct tggacggcaa
     5641 attccacgga catcaggaaa tgtttgtttt tctctacgtt tttatctttt aagtccatag
     5701 gcatctaaga tctctttctg taaaccaaac atttctttct catcctctgg tgtcatatgg
     5761 tcatacccgt tgatatgcaa gaatccatga accgctaaaa agccaagttc ccgatcatac
     5821 gaatggccgt aagcttctgc ttgctctgca gcacgttcag tagagatcat gatgtctccc
     5881 agtacacgag gcaatggatt ctctccatct tcaaaaatca ctgggatttc gtcttcgccc
     5941 tcttcttcca aggcaaaact gatgacgtct gtcggtgcat cttttccacg gtaatcacga
     6001 ttgattactt ggattgctgc attgtccatg aaagtaacag acatttctgt ttcttctggc
     6061 aactctaaat atgatgctgc aaattgcagc aagtcgtcga tttctttcat tttctcttct
     6121 gagacttggt ttgtttcatc catgaaggta atatccatta tacatcgctt ctttctattc
     6181 ttcttgctat tcttcttgtt cttcttttat cttctcaact tccgatttca ttttaggata
     6241 tttgatccga gaatggaagg tactgcttaa accgctgatc aatgattttt ctactttacg
     6301 gatttctttt aacgtcaatc cactgtcgtc caattgtcca tcggaaatac gctcttcaat
     6361 aagattatgg acaaaacttt cgattttttc gctcgttgga tggtccattg cccgtactgc
     6421 cgcttcacaa ctgtcagcta tattgacgat tcctgcttca cggctttgag gttttggtcc
     6481 aggataacgg aattgctctt ccgtaacttc cgggttgcgt tcctttgcct taacgtagaa
     6541 ataacgcatc agcgtggttc catgatgctg tctacaaata tcgatcacca tttgcggcat
     6601 attataatct tccaatattt tggcaccatc tgtcacatgt ccaaagataa tatgtttact
     6661 gtcttctggc aataagaaat tgtgcggatt ttccgctcct gaaggcaagt tctcaacgaa
     6721 aaagctggcg tgtttgatct tgccgatatc atgataatag caagctactc gagttagtaa
     6781 agaacgtcca ccgatttctg ctacagcatt agcacttaga ttggctacca tcatgctatg
     6841 gtgatacgta cctggtgctt cttccagcaa ctgtttcaat aaaggatgat tgggattgct
     6901 gagttcattt aatacgatca cactatcatc attgaccatc aattcaatat aggggtgcag
     6961 acctatcgtc agcaagaatg aaaacagtgc acctgtgaat ccgcagacta gcgttaacca
     7021 agtcgttgca tccccaaagc tcattccttg gtagataatc aaaatgacat ccatcatcac
     7081 aggaaaaata atgacccaca tagcagcgga taacccttgt tctgagatcc gtttgcgtct
     7141 gacgatcgtt gccatgagac cagaaaatac ataggacatc aaaataactg tcagaaagtt
     7201 cgtgccaatc gaatcataaa aaatgaaaag tgcggatact gcttgaaaaa taccagccat
     7261 gatccctgct cttcgattca agaaaaagct taaaacaagt ggagcaaaag ccgcggggta
     7321 aaacagcgga atataagcag cttgttctgt ttggaagagt tggaaaaatt tcattagtac
     7381 gacgcttact gacattgccg tcacgtagaa aagtacgtat tttgttcttt ctcctgcatt
     7441 ttggaattga agtgaattat aaatcagtac accgatttga agcaaaacag ctaaaaccat
     7501 ggcaatgagt gggaaaatag aagtcgtttg attcgtcaat cctagtaatt ccaatttttt
     7561 catcgctgaa gcatcaattt gatttccttc gcgaacaata atttctcctt ggaagatcat
     7621 cactggttgt acagcttctc gtgcagattg ttttaattct tctgtctttt tctcattcaa
     7681 tacatcatta actacgatgc cttgatccac taagtagcga atcatttgtt gctgctctgt
     7741 tgtgacattc aaatattgaa tctgatcttc tgcttcttgt ttaaaagctt ccaattcgct
     7801 ttcacgaact tgtttactca tctgttcgtc aatgagcttc aaactttcat ccctaactgt
     7861 gtgaatttcc gtttcagtca tttcaaatat cgtcgaataa aaattgttcg gcagtttttg
     7921 gtaaaacgca acgttttctg cattcacgtt ttcaaaattt ttcttcagtg tagcaatccg
     7981 ctcttctgta gtaggtttcg ggatattttc atcttccttc gccttatcta ccttgccatt
     8041 ataagatttg ttgatggtat cattcgtctt atcgatcaat tcgaataatt gcttgatccg
     8101 attgttctgg tcatctgcca gatctttttg ataagtatat tctggagtca ctgcttctgc
     8161 tgccagtttt cttctttgtt cggtttcttc cgtgttctca atcgttttat tcgcacggat
     8221 actctcttct gctacttggc cttctctgaa atcaatggcc ttttgtttga cactactaaa
     8281 tgtaacgccg cagacaatga taaaaaacaa gaacagaagg aaaggcaatg ctgcctttcc
     8341 gagtttttct tgtagtttct gcagtgtttt attcaacatg tgcttatcct cctaagcaaa
     8401 gcttattctt gatgaagatc cgccttttca taagctttga tgatttcggc aactacaggg
     8461 tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga tttttttgat ttgctttaat
     8521 gttcgttctg catgaacaag accacttgtc gtcccacgag gcaagtctat ctgacttgta
     8581 tccccattga caatcatttt tgattgataa ccgagacggg tcaagaacat tttcatctga
     8641 gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg catcatccaa tgtccgcccc
     8701 cgcatgtaag ctaaaggtgc gatttcgatc acgcctcgtt ccatcaatcg gttcgtatga
     8761 tccaatccaa aaatttggta taaagcatca taaacaggcc gcagataagg atccactttt
     8821 tctttcagat ccccgggtag aaagcctagg ttttcccctg cctccacagc gggccgcgtc
     8881 aaaatgattt tttgcacttc gccttttttt agggcagcaa ttgctaatac tacggcaaga
     8941 aacgttttcc ctgttccggc tggccctaca ccaaatacga tgtcatgttt ggctaccgat
     9001 tcaacgtatt tcttttgacc gctgtttttt acccgtatag gttttccatt acggtctttg
     9061 acgatctctt cctcatacat ctcaacaaaa taatctaatg tacctttttg ccccattttc
     9121 aatgccgtca caacatctgg cgtacttacg tggattcctc gtttgatcaa ttcctgtaat
     9181 gtccgaatga ctgaggcagc caaggaaatc tgggattgct caccaatgat ctgaatgatt
     9241 tctccacgtg tatggatggt tgtttccgtt gtttcctcga tcagttttat atgcttgtcg
     9301 tgtgcaccta aaagcatgct gacatcatca gcagctgtca atctaatctc taaagaacta
     9361 gactcttccg tcaaaaatag acatcccctt tttttcgatt cattctttcc tattttacca
     9421 tactcagaag aaactatata gcaaataaga aaaggttgta aaagagtcgc ttaaccatac
     9481 gtaccaagaa acgttaaaaa atagccgttt ccattcgcta aaggtctcgt tttgataaaa
     9541 agaagctgag aatgtaagtt atatgaaagt ggagaacaaa atatttcgtt tcattcaaca
     9601 agtattctct aggtattttt cctgtttcaa aaaaagaaaa caaaatagtt aaaatattct
     9661 tgcatttcaa aaaaaacagg tgtattatta cgtcaacaaa taaacataca gcaaagcgtc
     9721 gaggaaaaca gtaggctatt caacgattta ttcccagtga gtcatgtaag tgagaaatga
     9781 caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta gatcattgtg
     9841 gctgcctcca ttatcaggct agcggataac tttatcaaaa agcgtccgtg aatgaagaag
     9901 cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt cccaagtgga
     9961 aaagggcttt tttttatttt aaggtggtgg ttttcatgga atctgatatt agtggcatta
    10021 ctaattgctg caacgtttat atcgtcaatc aaaatgataa ttattggagg aaaaatttat
    10081 ggaagaaaca aaaacttatt catggaaaca actattgatg gtcagttcac ttatcttcgg
    10141 gatgttcttc gggtcaggta atctaatctt tcctgtccac ttaggtcaaa tggctggcgc
    10201 aaactgggtc agtgctggcg taggatttgc tatctcaggt gctcttttcc cattactagc
    10261 tatcttagct gtagtagtga caaaaagtga tggtctgtat gacttagcta aacctgtcgg
    10321 aaaatggtat gctgccttgt tcttaatcct tgttcatttg acgatcggac catttttcgg
    10381 tacaccacgg acagctgcta ctgcttttga aatggctgct aaaccatttt tgcctgaaaa
    10441 atataattca ttaggtatgt ttttattctc tgctcttttc tttttacttg cttatttcct
    10501 tactgtccat ccaacacggt tggtcaaata cgtcggaaag tatctaaact ccttgtttct
    10561 tgcattatta gcaatcattt ttgtcgtttc tttattccat ccaatgggaa atctaaatca
    10621 atctgctgca gatacttatc aaacaaacgc tttaacaaat ggtgtcctag aaggttataa
    10681 cacggttgat gcagtagctt tgcttgcctt aagtgtcact tttgtccatg cagtccgtga
    10741 ccttggtttc aaagataaaa aagtcacaga attaacggca aaagctggta gcctgagcgt
    10801 tgcgctagaa gtcatgatct attttggact tgttcttctc ggtgcgatga gtttgaataa
    10861 gatgtcttta tcagaaaatg gcggaactgc actttctcag atcactaatt tttatttagg
    10921 taacttcggc gctagcttct taggagtgat ggtcacatta ggtgtcttca ctaccgctat
    10981 gggactcgtt gtttcttttg cgcaagattt ccataaactg ttccctaaag tcagttatat
    11041 gacatggctg cggttaacga ctttcgtctc ttttgtcgtt gcaaatgctg gtttagacaa
    11101 tatcatccaa tggtctttac cagtattgat gcttttgtat ccattgtcgc ttgcgttgat
    11161 cttgctttcc ttaacagcaa aatttttcca aaagacacca tttgtctatc aagtaacgat
    11221 gttgtttgct gctgttcctg ccgtattgga tatgctggca aattcgcctg ctttagtgag
    11281 tcaacaaaga gttgtagcaa gcatgctcga attttatcat catcacgttc cttttgctgc
    11341 attaggactt ggctggatgg tcccaacgtt acttggttac gctggcagcc tactgtttta
    11401 ttacgcctat cgcctatcag ggtataaaca agaagcaaat gaactaccag aagaataacc
    11461 aaatcaaaca caatttaaca taagaaatat taaacaggaa aaaaccagct agatgaaaga
    11521 atcctattct ttcgtctagc tattttttag ttctcttttt tacgcgcaca aaaaaactgc
    11581 ggacaatgat tgtccgcagt ttaaatgggc tctataatat ctagtgttgg tgacgccttc
    11641 tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttga aatcctttag
    11701 aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc agagtttacc
    11761 aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc ctttcgcaaa
    11821 gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta tcaaccagag
    11881 gaatgcttcc attgtcacta tcaaaataag caaacgatca ttaaatgggg ctggaaaaaa
    11941 gtatccatct tattgaatga tgtcagtaac tacaagacca ttctgcggat taacaaacag
    12001 cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt tagtgatcgt
    12061 cgctgctcca ttgctcgcag agtcaaacaa gccattcttg agctattaag cgaacctctt
    12121 tctatgtctt tgatcgcacg tatgaagcac atttctccga ccactgtcat tcgaatactt
    12181 cgcagccttc ggcctaaaac cgtttctttg aaccagccgt taccagaagt ggtgtgtttc
    12241 gatgaattca aatcggtgaa aaacgtctcg ggagccatga gctttgtcat gatggacggc
    12301 aagacccatc aactgattga tattgtggaa aatcggcaac taaatccttt acgcgactat
    12361 ttcttgcgct accctagaaa agtccgtgaa caagtacgtc tagtggtttc agatttctat
    12421 acaccctatc gcacattggt gaaagagtgc tttccgaatg cccgaatcgt cgccgatcgc
    12481 tttcatatta gccaacacat cggtcgggcc tttaccaacc accgaattca agtaatgaag
    12541 acctttaaaa aaagggatcg tcggtcgaag cacttaaaga aatactggag actacttcaa
    12601 aagaatgcgt gggaattgaa aggtcagcat cgctactggc gtccgtcatt tcgagatcat
    12661 ctgacagaag cagagatagt cgatcggcta ttatcttatg atgactcctt aaagcgaggc
    12721 tacgaagtct accaggattt tctttccgct atcagaagac aagatgttcc tgaattcgtc
    12781 gcgctcctaa aagaagacta caaggagttg ccagagcact accaaccagt gttcacgacc
    12841 ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag ttccctattc caacggccct
    12901 atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa ttgcctatgg ctttagaaac
    12961 tttcaaaact accaagaacg aatcttttta tatcgaggaa aatattttaa gaaaactaag
    13021 aacactaccc aactgactaa agctagaaca acaacacggc tagacaagat agctgtctag
    13081 ccgtgtgagt atctcgatta ttctattgaa tttcggctct atactttttg atgttggtga
    13141 acttcaccaa catcttttt
//
LOCUS       DAYYNG010000072        13157 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000072 DAYYNG010000000
VERSION     DAYYNG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.072"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..879
                     /gene="sdaAB"
                     /locus_tag="ACHBFA_001660"
     CDS             211..879
                     /gene="sdaAB"
                     /locus_tag="ACHBFA_001660"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9395148.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            892..1764
                     /gene="sdaAA"
                     /locus_tag="ACHBFA_001661"
     CDS             892..1764
                     /gene="sdaAA"
                     /locus_tag="ACHBFA_001661"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9395149.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(1855..2955)
                     /locus_tag="ACHBFA_001662"
                     /pseudo
     CDS             complement(1855..2955)
                     /locus_tag="ACHBFA_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(3047..3934)
                     /locus_tag="ACHBFA_001663"
     CDS             complement(3047..3934)
                     /locus_tag="ACHBFA_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9395150.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(4011..4556)
                     /gene="nusG"
                     /locus_tag="ACHBFA_001664"
     CDS             complement(4011..4556)
                     /gene="nusG"
                     /locus_tag="ACHBFA_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9395151.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(4657..4827)
                     /gene="secE"
                     /locus_tag="ACHBFA_001665"
     CDS             complement(4657..4827)
                     /gene="secE"
                     /locus_tag="ACHBFA_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9395152.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(4851..5003)
                     /gene="rpmG"
                     /locus_tag="ACHBFA_001666"
     CDS             complement(4851..5003)
                     /gene="rpmG"
                     /locus_tag="ACHBFA_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9395153.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            5165..5809
                     /gene="cbpA"
                     /locus_tag="ACHBFA_001667"
     CDS             5165..5809
                     /gene="cbpA"
                     /locus_tag="ACHBFA_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM9395154.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(5865..6368)
                     /locus_tag="ACHBFA_001668"
     CDS             complement(5865..6368)
                     /locus_tag="ACHBFA_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9395155.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(6381..6427)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            6965..7930
                     /locus_tag="ACHBFA_001669"
     CDS             6965..7930
                     /locus_tag="ACHBFA_001669"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9395156.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(8029..9201)
                     /locus_tag="ACHBFA_001670"
     CDS             complement(8029..9201)
                     /locus_tag="ACHBFA_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395157.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(9188..10333)
                     /locus_tag="ACHBFA_001671"
     CDS             complement(9188..10333)
                     /locus_tag="ACHBFA_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395158.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(10347..11279)
                     /locus_tag="ACHBFA_001672"
     CDS             complement(10347..11279)
                     /locus_tag="ACHBFA_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395159.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            11396..12475
                     /locus_tag="ACHBFA_001673"
     CDS             11396..12475
                     /locus_tag="ACHBFA_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9395160.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            12472..13101
                     /locus_tag="ACHBFA_001674"
     CDS             12472..13101
                     /locus_tag="ACHBFA_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9395161.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
BASE COUNT         4014 a         2694 c         2389 g         4060 t
ORIGIN      
        1 tatttgtttt ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa
       61 tttattctaa aaaaacaaca atttttcgtt ctcatttctt cttttatgtt acacttgcgc
      121 tagcatggtt caacagatga aagcaaacaa taacacggtt ttttagaccc ttgtacaaag
      181 attcctacat aacaaaatga ggtggagaaa atgaaagatt taaggtatag aagtgtcttt
      241 gatattattg gacctgtgat gattggtcca agtagctctc acactgccgg agctgctaga
      301 atcgggaaaa tcatacgtag tatatttggc gaacagcctg attctgtaga tatttatcta
      361 tatgaatcat ttgcaaaaac ctatcggggg cacggaacag atattgcatt agttggcggc
      421 ctgttaggga tggaacccga tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa
      481 gccggtatgg aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc aaactctgta
      541 aagatgcttc taaaaaaagg aacacgaaag ctttcagtca ctggcatttc gatcggcggt
      601 ggcaatatcc agatttctga attgaatgga ttcaaaattt ctttaagcat gggtactcct
      661 accttgatcg ttgtccacca agatgttcca ggtatgattg ctaaagtgac tagtatactt
      721 tctgagacca atacgaatat cggcactatg acagtgacac gggaatcaaa aggtgaaaat
      781 gccattatga taattgaagt agatgatcct caagtagaag aaacggtaaa aagactcaaa
      841 cagctgccaa atatcgacag cgtaaattat ttcgattaag gagctaaaaa aatgttttta
      901 tcgattgaag aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt
      961 gcagttgaaa tggaaatgag tggccgttcc cgtagtcaca tcatcgagat catggaaaaa
     1021 aacctctcag taatggaaca atcaatcaac gagggaaaag aaggtgttac ctccgtcaca
     1081 ggaattaccg gtggtgatgc aaaaaaatta gatgattatt taaatgaggc agacttttta
     1141 agtggagaaa caatcttaaa agctgtacgc aatgctgtag ctgttaatga agtcaatgca
     1201 aaaatggggt tgatttgtgc tacaccgact gccggaagcg cgggtgttgt tcctggtgtg
     1261 ctgatggctg tccgagatcg gcttgatttg acacatgaac agcaattgga attcttattt
     1321 accgctggcg ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg
     1381 ggatgtcaag cagaagttgg ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca
     1441 aaaaatggga ctcctcatca agcagcacaa gcagtagcca ttacattgaa aaatatgatg
     1501 ggactgattt gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct
     1561 ttaggctctt ctcaagcttt tatctctgct gatatggcat tagcgggtat cgaaagtgtc
     1621 attccgccgg atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt
     1681 ttcaaagaaa cagcagaagg tggcttagct gttacgccaa ctgctaaacg acttgcggaa
     1741 gagattttaa ataagagggc gtgataaagc tccttaattt cgagtattaa gagattagtt
     1801 atcttcataa caaaaaacga agagaggact aattgccctt ctcttcgttt tttattaatc
     1861 catcaatttc gtatttttta cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat
     1921 cgacaattct ttgttcaggt gttcaaaaac agatgttact ccttctgccc cacctagatt
     1981 caacccataa atcactggtc ggccaatcgc aactaagtca gcaccacttg ccagagcttt
     2041 gaagacatgc tctcctcgac ggacaccaga gtcgaaaatc actggtacac gtttgttgac
     2101 tacttcggca atttttggca atacttcaaa ggacgcagga cctccatcta attgacgtcc
     2161 accatgattg gatacccaga taccatctgc ccctgcagag atagcgatta ctgcatcttc
     2221 tggcgattgt attcctttta cgataactgg aagatgggtg atttctttga ttgttttgat
     2281 gtcatctggt gtcaagcctt gttttgcagc tgcataaatt tctgcgattc cttttccttc
     2341 gccattacca cttgcacttt gttcgctata cgcagctaag ttaggcattg gtaatggaaa
     2401 ttggaattga ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa
     2461 aatgatagct ttagcccctg cttctactgc tttgttcaaa atgaactcat tgaatctgtc
     2521 gtctttactc atatataatt ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc
     2581 tttgattgtc gtatttgcat aagtgctgat ggaaaagatt gatccagcag cagctactcc
     2641 tttagctgta tcagcttccc ctttttcatg tgccaatcct tgggccgctg atggcgcttg
     2701 gataatcggt gtatctaatt caattccgaa aacagatgta tgcaaatcgg ctgagtcgat
     2761 tccgcgtaat atccgtggca tgattttctt tgtattaaaa gaagtcgtat tttctttcat
     2821 cgtccactca tcttcagaac caccgcgaat atagccaaaa gctcctgctt ccatgcgctc
     2881 ttttacacga ccttctagtt aggcaatgtt tacaatatcg attggatgtt cttcattact
     2941 tgtatgataa gtcatagtag atccttcttt catcttcttg attacatata aatcatacta
     3001 aacttacaaa aagtaagcaa acatttactc tcagatactt ttatcgctat tctttccaaa
     3061 taaatcgact tacaagctga tctacttctt ggttttcatg aagcatacta tgagaggcat
     3121 tttcacctgt cactactttt tcctctattt catttccgcg ttgcttcaac aacgcataga
     3181 ccgctaatgc actgtttaac ggtactgttc catcactcaa gtctgtttca tctagttgac
     3241 cagcaatcaa gaaaaatcga gtggttatag gaatattacc gatcatttgc tggtaatctt
     3301 gatagcggtt agaaacgaca gcaggcccct ttgctaactc atctgtcaga gactgcgcac
     3361 tatcatcgac aaaatcatta aaaggcgcgc caattgctgt taatgtctga acttgtggca
     3421 atgacgtatc ctgtccatac gttcctaaat agcgtagtgc actaactcct cccatggaat
     3481 gccccactat gttgacttta tctatgccgt agttttcttt cagatacaaa agacaagttt
     3541 tgatccattc tgcttgattc cactcgttat ttttattatc ttcaaacaat acttggacca
     3601 tcggattgtc tgcttttcca gacaaatctc ctgttgcttg tatttgtcca tctgttccta
     3661 ctgttaaaac caattctttt actgcttgct gatttttttc caaacgccgc aacatccctt
     3721 taaaggaatt ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt
     3781 ctatcacttt actggattct gccgtttttt ctttatcaga aacttcagta gtatttatct
     3841 tcgtcgtctg gcaaccactt aacgcaaaca aactgataag gactaaagct gctgcagttg
     3901 cacgtttgca taaatttttg attggttctg tcatacacct cttcctttcc atatagaaaa
     3961 aagaaagact tgaaaggcca atcatatgat cgacatttca agtctcttta ttaatcgatt
     4021 ttatctactt gatcgaaatc aagttctgta ctggtttcac gtccaaacat atcgatgttg
     4081 actttcaatt tttctttttc tccgtcaact tctgttactt gtccttctaa tccagcaaat
     4141 gcgccttcga tgattttcac gacttcgcca acttctactt ctagatcatt ttgtcgcagg
     4201 ctcataccga tagaacgcaa gatatgattg acttcttctg gaagtaatgg tgctggtttg
     4261 cttccggctc cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg gacaacatac
     4321 catgaagcgt ctgtcatgat catttcaaca agtacgtatc ctgggaatgt tttatgaacg
     4381 atttcttttt ctttcccatt tttgacttca gtttcttttt cttcaggaac aacgacacgg
     4441 aaaataaagt cggccatttt catactttgc gcacgagatt cgatatttgc ttttactttg
     4501 ttttcatatc ctgaatatgt atgcaagaca taccagtttt tttcaaaaga ttccattgaa
     4561 ggctcctcat ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt ttctttcgct
     4621 tcattatagc actaaaagta cgttgtggct agcgagttat tttaaaatcc agccaaataa
     4681 gctttggatg gctgtatcca ttacgtaaaa catcaatccg aaaataatag aagtttcgat
     4741 gactactaac gtatctttac ggagttgttt tctgcttggc caagttactt gtttgatttc
     4801 atcttttacg ctttttagaa atttcatgag acaacctccc ttgctactgt ttatttcgtt
     4861 tctttatgta atgtatattg attacaatat ttacaaaact tattgatttc caaacgttcg
     4921 ccgctttttc cttcactgac tgattttgtg tagttgcgag acccacaaac ggaacaagcg
     4981 agtgctgctt ttttctttgc catttacttc tgcccccttg ctgagaatca gacttatact
     5041 tcataacagt atcacgtaac tttttacttg tcaatttgtg aaataaacta tctattgttt
     5101 tggataaaat tatgatatga ttaatttgaa attaagctaa aatgagacaa ttggaggtat
     5161 agcgatgcta ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac
     5221 tagtacatta gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat
     5281 cttagattct tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca
     5341 taaagcaaat ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa
     5401 gttcatttac ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta
     5461 tattgctgtc cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct
     5521 taatatgctc tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc
     5581 cacaggaaag caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat
     5641 tgccagctgt attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac
     5701 gctaccggct aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa
     5761 gaattataaa gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga
     5821 aaaaagaaaa cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca
     5881 aaatcaagcg cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac
     5941 tcacttaatg caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac
     6001 atataagcaa tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc
     6061 tttacatttt tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg
     6121 ccgccaccaa aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc
     6181 ccgcctaaaa ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct
     6241 gaaatacgaa actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga
     6301 taaagagcca tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt
     6361 ttttgcattt tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg
     6421 cggttatgtg aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat
     6481 ctgcttttaa aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat
     6541 ggtataaagt aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct
     6601 gcaagaacag aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa
     6661 cctcttacta gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt
     6721 ggaacttctc cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg
     6781 gtcttcggac ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt
     6841 aacagatttt tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat
     6901 tattggttaa ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga
     6961 aatcatgaaa ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta
     7021 taaagtagca gaaagaattt taccggtatt aaaaaataag catattgact atacacttca
     7081 ttatagtgaa tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt
     7141 gattccttgg atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt
     7201 aggaggtgac ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt
     7261 cccagttgct tatattccag caggttctgg aaatgatttt gctcgagggg caggccttcc
     7321 aaaaaatccc aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca
     7381 cataatggct tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg
     7441 tataggatta gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt
     7501 gaacaaatac aacttaggat cattttcgta tttattctct attctccgtg ctttattcac
     7561 tcaaaaagga tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc
     7621 atttttatgt acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac
     7681 tgctgatgcg tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa
     7741 aatcttgtgg ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca
     7801 tcactacaca acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga
     7861 tggagaagaa atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt
     7921 ttggtgttaa taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa
     7981 aaaggactgt gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc
     8041 ccaattgggt tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa
     8101 caccgcagca aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg
     8161 attgtaactg agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt
     8221 gaatgccgct attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc
     8281 gataaagcaa ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt
     8341 taccaatgaa gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt
     8401 taaaatatga taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag
     8461 aagtaagaag ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg
     8521 attcaagttt cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt
     8581 aaacgctaga ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa
     8641 gaaattaaag gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag
     8701 ctcaacttct ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt
     8761 tttatcttct tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac
     8821 aactgtttta gaaaaggcat ctgggcgact ctgtatcttg atcgtcgcca tctttcctac
     8881 ttcgacttgt tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata
     8941 aagagcatca tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc
     9001 attcaaatat ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat
     9061 tttcgcatct tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc
     9121 aacaacagtg ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca
     9181 aatcgtttta taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa
     9241 aaaggaaaat aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt
     9301 agaaataaag tttatacaaa ctttcgctga tcaagttgac gagatttaat tgaccaacaa
     9361 tcggaatatg aagatcaatc caatacttca cttgttccga ccccatcata ccagcaaaaa
     9421 agctcatcgc aatcgtcact gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg
     9481 gtatcaaatt accaaccaat gtacctaaca aaatcgcaca caacgacccc agagcggtaa
     9541 caatcatcaa ccattgccaa cgttcaccaa agtctacttg atagataaaa cgaaagacag
     9601 ctaacataat ataaatctct gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg
     9661 aaactaacga ttgctttcta ggaatcaagg acaagcgaat accatttgac gattgatttg
     9721 cttgctgatc ctgtacattg cggacccccc acatcgtccc gtagagaatc gccattccaa
     9781 tcagagtgaa gaaataaaaa cttttgaaac tcatattacg acctgcatga acttcttgaa
     9841 aagatacttc ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc tccacttctg
     9901 ccactttact cgaaattata ccaatacgct gtaaaaattg atccatgacg tttttcatca
     9961 acgtctgact cattcctgct tgacccacat ggagcgaaac agatgaacca tcaatgagat
    10021 aaaaaccagc caaatcatct gcatttagct tttcattcgc ttctacttcc gatagttcct
    10081 gtacttcaaa taatggttta tcattgactt cgacttgttc taagactgtg gtaaaatttt
    10141 ccgaaagcgc tgtttgatcc gctttgatta caattccagt attaatcgtt tcaagattgc
    10201 caccttgatc aatctcgcca aaggccgcca taaacatcaa tcctaaaaga attggaaagg
    10261 ccaatgtcca aaacaacatg gatttatttc gcagcagtat ttttgtgcga tatttataaa
    10321 aatgccaaaa cattatttcc cctcctctag tctctcaaat ccttgccagt aatctctaaa
    10381 aagacatcat tcaaggacgc ttctttggtt gctatttttc cgtaactgat cttttcttct
    10441 tgtaagattg taagaatcgg caacaatact gtatttagtt cagtagttgt tacttggagc
    10501 tgaccttttt tataagcgat tccatcagaa gctattgttc gctgaatcgc tgacaacaca
    10561 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    10621 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    10681 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    10741 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    10801 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    10861 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    10921 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    10981 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    11041 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    11101 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    11161 ccactgccgt ttggacctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    11221 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    11281 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    11341 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    11401 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    11461 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    11521 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    11581 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    11641 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    11701 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    11761 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    11821 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    11881 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    11941 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    12001 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    12061 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    12121 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    12181 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    12241 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    12301 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    12361 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    12421 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    12481 gtagtcatta ttgatgatga tccatttgtg acgaaatcgt tacaaaccat tttagaaagc
    12541 acgaaagaga tccgtgtgtt aggcattggt cattgtgcca aggatgcgct agtcttatac
    12601 gaaacccatc gtcccgatgt tttgctgacg gacatccgca tgcctaaaca aacgggcatc
    12661 gatgctgcca aagaaatttt ggctaaattt cccaaagcca tccttttgct tttgacgaca
    12721 ttcaaagatg aagaatacat ccgcgaagca ttttccatcg gtgtcaaagg gtatttaatc
    12781 aaacagaact tgcaagcgat tattccttct gtcaaatccg cttataatgg acaggccgta
    12841 tttggtaatg aaatcatcga gactttcact caaatgatga agaataaacc gacgattgat
    12901 agtcatgctt atgcttcttt tagtgaacgt gagcttgcaa tcatcaaaga agtagctgcc
    12961 gggaaaaaca ataaagaaat tgcagatgcc ttgtacttga gtgacggcac tgttcgcaat
    13021 tacatcagcc aattactaga aaagctagat ttacgggatc gcacgcaatt ggctatctat
    13081 ttttatcagc atttgcaata agtcttatct aacacaaaaa aacctgaatg atgctccttc
    13141 gcatcattca ggttttt
//
LOCUS       DAYYNG010000073        13014 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000073 DAYYNG010000000
VERSION     DAYYNG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.073"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1717
                     /gene="yfcC"
                     /locus_tag="ACHBFA_001675"
     CDS             <1..1717
                     /gene="yfcC"
                     /locus_tag="ACHBFA_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM9395162.1"
                     /translation="KPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGKFSTQDIEY
                     KDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPEKGLEKVIA
                     DGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGGFGFLNFVF
                     EGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLERLALPLLF
                     FAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNATSWMSPFS
                     VAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLTYKSDDYFR
                     SHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFFVMGLVAGI
                     IAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASVPSALNTIL
                     HGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADLVGVSRQVA
                     VLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLGTIFIMIAI
                     AIGLQ"
     gene            1874..3058
                     /gene="iadA"
                     /locus_tag="ACHBFA_001676"
     CDS             1874..3058
                     /gene="iadA"
                     /locus_tag="ACHBFA_001676"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM9395163.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3169..3372
                     /locus_tag="ACHBFA_001677"
     CDS             3169..3372
                     /locus_tag="ACHBFA_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9395164.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3473..4135)
                     /locus_tag="ACHBFA_001678"
     CDS             complement(3473..4135)
                     /locus_tag="ACHBFA_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395165.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4148..5650)
                     /locus_tag="ACHBFA_001679"
     CDS             complement(4148..5650)
                     /locus_tag="ACHBFA_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395166.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6198..7877
                     /locus_tag="ACHBFA_001680"
     CDS             6198..7877
                     /locus_tag="ACHBFA_001680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9395167.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8004..8483
                     /locus_tag="ACHBFA_001681"
     CDS             8004..8483
                     /locus_tag="ACHBFA_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9395168.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8702..9382
                     /locus_tag="ACHBFA_001682"
     CDS             8702..9382
                     /locus_tag="ACHBFA_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM9395169.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9390..10721
                     /locus_tag="ACHBFA_001683"
     CDS             9390..10721
                     /locus_tag="ACHBFA_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM9395170.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10736..11698
                     /locus_tag="ACHBFA_001684"
     CDS             10736..11698
                     /locus_tag="ACHBFA_001684"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9395171.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11809..12147
                     /locus_tag="ACHBFA_001685"
     CDS             11809..12147
                     /locus_tag="ACHBFA_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9395172.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12226..12933
                     /locus_tag="ACHBFA_001686"
     CDS             12226..12933
                     /locus_tag="ACHBFA_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM9395173.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4122 a         2307 c         2867 g         3718 t
ORIGIN      
        1 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
       61 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      121 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      181 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      241 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      301 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      361 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      421 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      481 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      541 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      601 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      661 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      721 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      781 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      841 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      901 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
      961 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1021 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1081 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1141 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1201 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1261 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1321 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1381 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1441 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1501 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1561 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1621 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1681 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1741 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1801 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1861 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1921 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     1981 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2041 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2101 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2161 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2221 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2281 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2341 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2401 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2461 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2521 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2581 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2641 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2701 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2761 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2821 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2881 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     2941 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3001 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3061 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3121 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3181 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3241 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3301 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3361 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3421 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3481 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3541 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3601 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3661 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3721 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3781 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     3841 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     3901 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     3961 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4021 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4081 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4141 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4201 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4261 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4321 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4381 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4441 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4501 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4561 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4621 tgataatacc ggctacagtc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4681 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4741 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4801 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     4861 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     4921 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     4981 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5041 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5101 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5161 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5221 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5281 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5341 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5401 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5461 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5521 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5581 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5641 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5701 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5761 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     5821 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     5881 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     5941 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6001 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6061 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6121 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6181 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6241 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6301 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6361 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6421 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6481 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6541 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6601 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6661 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6721 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6781 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     6841 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     6901 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     6961 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7021 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7081 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7141 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7201 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7261 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7321 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7381 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7441 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7501 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7561 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7621 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7681 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7741 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7801 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     7861 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     7921 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     7981 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8041 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8101 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8161 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8221 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8281 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8341 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8401 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8461 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8521 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8581 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8641 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8701 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8761 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     8821 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     8881 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     8941 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9001 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9061 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9121 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9181 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9241 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9301 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9361 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9421 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9481 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9541 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9601 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9661 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9721 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9781 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     9841 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     9901 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
     9961 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10021 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10081 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10141 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10201 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10261 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10321 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10381 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10441 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10501 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10561 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10621 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10681 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10741 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10801 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    10861 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    10921 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    10981 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11041 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11101 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11161 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11221 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11281 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11341 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11401 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11461 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11521 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11581 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11641 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11701 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11761 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    11821 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    11881 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    11941 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12001 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12061 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12121 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12181 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12241 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12301 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12361 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12421 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12481 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12541 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12601 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12661 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12721 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12781 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    12841 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    12901 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    12961 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataa
//
LOCUS       DAYYNG010000074        12491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000074 DAYYNG010000000
VERSION     DAYYNG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.074"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..946
                     /gene="mltG"
                     /locus_tag="ACHBFA_001687"
     CDS             <1..946
                     /gene="mltG"
                     /locus_tag="ACHBFA_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9395174.1"
                     /translation="LVQVEIPSGSSNKQIGEILEKDNIIKSGIVFNYYTKFKNLTGFQ
                     AGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPEGYDIDQIAERVAKVTGKDK
                     KEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEGYLFPATYDYYTSTSLKDLV
                     IEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEGVKENDRKNIAQVFFNRIKA
                     NMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTGYGPGPFDNPSEEAIKAVLE
                     PAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            1082..1561
                     /gene="greA"
                     /locus_tag="ACHBFA_001688"
     CDS             1082..1561
                     /gene="greA"
                     /locus_tag="ACHBFA_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9395175.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            1729..2460
                     /gene="liaF"
                     /locus_tag="ACHBFA_001689"
     CDS             1729..2460
                     /gene="liaF"
                     /locus_tag="ACHBFA_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9395176.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            2457..3524
                     /gene="liaS"
                     /locus_tag="ACHBFA_001690"
     CDS             2457..3524
                     /gene="liaS"
                     /locus_tag="ACHBFA_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM9395177.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            3531..4163
                     /locus_tag="ACHBFA_001691"
     CDS             3531..4163
                     /locus_tag="ACHBFA_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9395178.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4303..4968
                     /locus_tag="ACHBFA_001692"
     CDS             4303..4968
                     /locus_tag="ACHBFA_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9395179.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            4996..5289
                     /locus_tag="ACHBFA_001693"
     CDS             4996..5289
                     /locus_tag="ACHBFA_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM9395180.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5357..6304)
                     /locus_tag="ACHBFA_001694"
     CDS             complement(5357..6304)
                     /locus_tag="ACHBFA_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM9395181.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6482..7285)
                     /locus_tag="ACHBFA_001695"
     CDS             complement(6482..7285)
                     /locus_tag="ACHBFA_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9395182.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7431..8345
                     /locus_tag="ACHBFA_001696"
     CDS             7431..8345
                     /locus_tag="ACHBFA_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9395183.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8393..8863
                     /locus_tag="ACHBFA_001697"
     CDS             8393..8863
                     /locus_tag="ACHBFA_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9395184.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            8948..9556
                     /locus_tag="ACHBFA_001698"
     CDS             8948..9556
                     /locus_tag="ACHBFA_001698"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM9395185.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            9690..9890
                     /locus_tag="ACHBFA_001699"
     CDS             9690..9890
                     /locus_tag="ACHBFA_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9395186.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            10644..11870
                     /locus_tag="ACHBFA_001700"
     CDS             10644..11870
                     /locus_tag="ACHBFA_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9395187.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYIPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(11978..12448)
                     /locus_tag="ACHBFA_001701"
     CDS             complement(11978..12448)
                     /locus_tag="ACHBFA_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM9395188.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         4292 a         1928 c         2563 g         3708 t
ORIGIN      
        1 attagtccaa gtggagatcc cgagtggctc ttctaacaag cagattggtg agattttgga
       61 gaaggacaat attattaaaa gcgggattgt tttcaattat tatacaaaat ttaaaaattt
      121 aactggcttt caagctggct attatcagtt agcgcctaat atgactcttg acgagattgg
      181 caaacagctg caagaaggcg gcacttctga gccaacaaaa gttgcagatg ggaaaattgc
      241 cattccagaa ggctacgaca tcgatcagat tgctgagcgg gtagcgaagg taactggtaa
      301 agacaagaaa gaatttttag atttagtaaa tgatgagact ttctttaacg agttgtatca
      361 aaaattccca gaattgttgg aaagtgcctc taaagctgag aacacaaaat accgtttgga
      421 aggctactta ttcccagcta cttatgatta ctacacgagt acttcgctta aggatcttgt
      481 gattgagatg gtgaacaaaa caaatacggt catgcaaaac tattattcag ctatcaaaca
      541 gaaaaatttg actgtacaag aagttttaac tttggcttct ctagttgaaa aagaaggcgt
      601 aaaagaaaat gatcgtaaaa atattgcaca agtcttcttt aaccgaatca aggcaaatat
      661 gccacttcag tcagatatct ctgttttata tgcattagga gaacataaag aattggtgac
      721 gtatgaagat acagctgtag attcaccata caatttgtat accaataccg gctatgggcc
      781 tggtcctttt gacaatccaa gcgaagaggc aatcaaggca gtattagaac cagcagaaaa
      841 tgactattat tatttcgtag cagatacgag cactggaaat gtttatttcg ctaaaacgta
      901 tgaagaacat atggaactag tgcaaaaata tgtaaataat tcttagaaaa acaggggcta
      961 gtcattgatc atttttagaa aaacatttac attttaagaa aagcgtggta atcttttttg
     1021 gactatattc ctcaaaagat taccttttgc ggtattttat cgaaaaaagg agacgttaca
     1081 catggtagag aaagtttatc caatgactct agaaggtaaa gaaaaattag aaaaagaatt
     1141 agaagaacta aaaacagtta aacgcggaga aatcattgaa cgaatcaaaa tcgctcgcgg
     1201 tttcggagat ctttccgaaa actccgaata cgaatcagct aaagatgaac aagcatttgt
     1261 tgaaggaaga atcacaacaa ttgaaaatat gatccgtttt gcacaaatca tcgataatga
     1321 cggcgtagat gcagatgagg tatcgatcgg taaaacagtt acgttcatcg agttgcctga
     1381 tggagatgaa gaagagtaca cgattgtcgg aagtgctgaa gcagacccgt tttctggaaa
     1441 gatttccaat gattctccta tcgcgcaagc attgattggc aaacgtaaaa atgatgaagt
     1501 aaccattgct acaccaggtg gcgatatgtt agttaaaatc gtcaaagtag aacaagcgta
     1561 gtctgactga aagatctaga gagttcatct tactgatgac gcactctaga tcttttctct
     1621 ttgagtattg caggtttcac tgaacgatct tacatctaaa gtcctatagc caacagtcaa
     1681 aaaaataggt atgatgaaat gagaatgtgt caggagggag aaaaagcgat gaatagttct
     1741 tggcgttttt ttgtggtagt cgaagcactg ctgctgttat ttgctgtttg gcaaatcgta
     1801 aacaatactg gacttttgct tttagtaata ttcggtattt tcaatatata tctagctatg
     1861 cgcaaatatc ctcgtactaa atttcaaaat tttcaattga tcttagggag cttggtcatt
     1921 ttttttagct tagtcaacag tcctgcttta tggatgatgg ttgtattggc cgttttattt
     1981 atcgggctga aaggtgtaga gatttcagga atagatttga caaaaaatgc tttttggcga
     2041 aaaaaacaga tcatgatggt ccaaacagag caattaaaaa cacataataa tgagcgtaaa
     2101 agacagcaat tatttggtaa ccaacgtatt ggaaacgacg tctatgaatg ggatgatatc
     2161 aacattgcca tcatttctgg tgatactatc atcgatttgg gcaatacact tttgccaaaa
     2221 gatgataata ttgtgattgt aagaaaaggg ataggcagaa cacggatctt agtacccctg
     2281 ggagtggcta ttcgtttaga gcatgcgaca ttagtaggaa atgtcttgtt tgaagaagag
     2341 cagttttcct taaaaaacga acagatcaaa atctgcagca gtgattatga tgaaaatcct
     2401 cgcagattga agatcattac caatactttg gttggagatg tcgaggtgat acgaatatga
     2461 tgggaagaat atccaaagca atgctagctg tttattcggg gattgcggct ttccttatta
     2521 ttctattttc acttttcact tatttttatg ccagtaacca aagtcattgg tggggagaat
     2581 tgctgcgtgc gcgtttatta tacgttccgc ttatcttcca tttgatggca atttcattag
     2641 gtgtaggatt gatcgtattt ctattgttat cacttattca aaaggcaaaa tacgggaaaa
     2701 tcgaagaaaa gcttcgtgca ctttcttctg gcaattatga atccaaactt ttgcatcagc
     2761 cgatcccaag cgcgtcggat gatctgtaca tcaaagatat tgatatggaa attactaaaa
     2821 taaaagaaaa aatgatcgaa gtatccagtg aattacagat tatgactagc cgtcctcaat
     2881 atgtagatgg acaaaccaaa gaagagattt taaaattaga aaggcatcga ttagctcgtg
     2941 agcttcatga ttcagtttca cagcaattat ttgcagcgat gatgatgatg tcggcattga
     3001 cggagcaagc agaaaaaagt gagacaccag aaatgttccg taagcagttg aaaatggtgg
     3061 cagagatcat caacgcttcc cagtctgaga tgcgtgcgct gcttcttcat ctgcgtccag
     3121 tcaatttaga agaaaaaagt ttaaaacaag gcattgagca gttattgaag gaattgcaga
     3181 ataaaatcca gatttcgcta aaatgggagg tagaagatgt aaaattaact agttccattg
     3241 aggatcactt gttccgaatc gttcaagaat tgttatcaaa cacattaaga catgctaagg
     3301 ctaatgaatt agaagtctat ttgcataaaa tagataacaa tcttttatta cgtatcattg
     3361 acgacgggac aggatttaat atgaatgaaa ccaaaacggg aagttatgga ttgaacaata
     3421 tcaaagaacg agtagctggt attggtggaa cagtaaaaat cattagtttt aaagggcaag
     3481 gtacgagcgt ggagaccaag gtccctttga tgaaggaggc atagataaag atgataaaag
     3541 ttttattagt agatgaccat gaaatggtac gcttaggcgt ctcttcttat ttatctatcc
     3601 aagaagacat tgaagtgatt ggagaagctg aaaatggacg tcaaggctat gaaaaggcga
     3661 tggcacttcg accggatgtc attttaatgg atctagtcat ggaagaaatg gatggcatcg
     3721 aatcaacaaa agcaatctta aaagattggc caaaagccaa aatcattatc gtcacaagtt
     3781 ttattgatga tgaaaaagtt tatcctgcaa ttgaagcagg agcagcagga tatttgttga
     3841 agacgtctac agcacatgaa atagctgatg ctataagggc tacgcaacgc ggcgagcgag
     3901 tactagaacc tgaagtgaca acaaaaatga tggaaaaaat gagtcggcga aatgatccag
     3961 tattgcatga agaattaacg aatcgagaaa atgaaatttt gatgctgatt tctgaaggga
     4021 aaagtaatca ggaaatcgcg gatgaattgt ttatcacact gaaaacagtc aaaacgcatg
     4081 tgtcgaatat attagctaag ttagaggtag aagaccgaac tcaagcagct atctatgcgt
     4141 ttaaacatgg tttggtgaaa taaaaacgat gataaagagg ataatcaaaa gctgagaaaa
     4201 taactattat ttttcttggc ttttttacaa taatgatgct tgcttttgtt tttggaaagg
     4261 ctttgttata ctgagaaaag aagatactaa aggaggaaaa tggtgaaaca aaattttgca
     4321 attgtaggcc ttggacgatt tggcggaagc atctgccgta cactgatcga agctggacaa
     4381 gaagtactgg ctattgacag taatgaagat cgagtcaatg aatatatgaa tatagccacc
     4441 catgctgttg tggcaaatgc acaggatgag atgacacttc gttcattagg tataagaaat
     4501 ttcgatcatg taattgtcgc tattggagaa gatatccaag ccagcatctt agttactctg
     4561 atggtaaagg aaatgggcgt accgaatatt ttagcaaaag cccaaaatga gtaccatgcc
     4621 cgtgtactag agaagatcgg agcagatcgt gtcgtacacc ctgagcgaga catggggata
     4681 cggattgcac ataacttagt atcaaagaat attctagact acttagagct gtcagataaa
     4741 tattctttag cagaaatccg agtctccaat cctaaatttt tcaacaaaac attagctgaa
     4801 ttaaatttca ggcaacgttt tgacttaaca gtagtcgcca tacgaagagt agatcaaacg
     4861 gttatcgctt ctcctgcagc agatgaatat gttcgtgaaa atgataatct actggttatt
     4921 ggagagacag gcgatgtgga tattctagac gataagatga atcaatagaa agaaaagaga
     4981 aagtaggttg ttgggatgaa attaacggta acgccaaaag cacaaaaatg gtttgactca
     5041 gaaattgatc tgccagaaac ttacggaata cgtttttttg gaaaagtcta cggcaaaaca
     5101 gaggttcatg atggtttttc gattggaatg tcagttgaac agccagaaca tcctgtaaag
     5161 aaagtggaaa tggatgggac actttttttt atcgaggaag cagatgaatg gttctttaaa
     5221 ggatatgatt tacttgttga ttatgatccc aacctggagg agccaaccta tcactttaaa
     5281 aaacaataaa aaaagagagg gtgacacaat ttttgtcacc ttctcttttt tcttttgaaa
     5341 atattgtttt cagctattaa tatcctgcag acaattttac ttgattggtt tgaagctcat
     5401 gggtggcaaa gtagcttttc agattggtta aaaaaatagc cattaatttc ttttgaaaat
     5461 caggtgtcaa acctgcaatg tgggaagtaa tcagtacatt gtccattttc caaagaggat
     5521 gatcttgtgg aagaggctct tcctcaaaaa cgtctaatgc tgcaaaggaa agttgctggt
     5581 tatttaaagc ctgcaccaga tcttgtgtct taacagaagc accacggcca acattgatga
     5641 atacagattt tggatcaaag tgttcaaaca tatgctgatc aaagagccca gttgtttcct
     5701 ccgttagagg taagatattg actacgaaat ctacttcagg aagaacttcc tttagttgat
     5761 ttaaagaaaa agtcttttcc attccttctg ctggatgacc ggatgtattg attccatagc
     5821 agttcatgtc taatgctttg catttttttg caatcatttt tccaatatgg ccagttccaa
     5881 tgattagtat ttttttccct tggatagatt ggtaatggac cgatgaacct aaccatttca
     5941 tgtcactctg tgccttttgt gcttgaaaaa gaccacgaga atacccaagg atgattccta
     6001 agacatgctc cgtaatcgat tcactgtgga tgccactacc atttgaaagt aaaatatgtt
     6061 tctttgaaaa agtctcaagg ggtagctgat caacaccagc tgagatagat tggacccatt
     6121 tcaaggtaga agaggctaat agtttttctt ggtagtctga gttccagccc aaactgatct
     6181 ctacattttg tagatcctct ggttgtaatt ctgttttaaa gatataacca ggcgctatag
     6241 tatggatttc ttcgataaat tcatcacgaa atggacgatc tgcataaatg attctttctg
     6301 acatagttaa tttcccgcct ttctttactc taagtgtagc gcatacaaga agaactttga
     6361 agcgccttgc ttgttttttg agaaagaaat atttttcaaa taaaaaaggg gctgtgacaa
     6421 aaaaatgtca caacctctaa aagggtctat tgaagtagaa ataacgagtt gtctttattt
     6481 cttaattgat cgatattttt gcccatcctt tgacagcttg agcaaaaata aagattcctg
     6541 caaaagcaat gaaggcgcta gtgtcaaaag aaggaagaaa gaccatcaag attgttgcaa
     6601 ttatagtagg caatgaaatt gaatagatca atgtttttaa acaatcaaga aaagtagctt
     6661 ttgttagtcg aaagcgtgca tagagaaaag cagcaatgtt ggtaaatagc aaagtgatca
     6721 gcagattcag aaaactagga taaatagaaa atataaatgt caggactttt atccaaagtg
     6781 gtatagatgc ttcacttaaa aaagaacgga gctgcttacc tgttaagttt tttaaagatt
     6841 cattggtgta gggaagttct aattggttat tgttcaacaa agcagaagtc gtgcctgtat
     6901 taggaagagc tatgataagt ttgtttttta acatcccgac actaacaaaa ttccccatca
     6961 aatcagtaga gatgtcctgt tctgttcgtt ttccctctgg atcaaatgtg aagataatgg
     7021 agtttgtttg gtaaatgaat ccttcttttt cttcggtttg aagctgcccg ttttggattg
     7081 taaaatcagg aatgcgagta gcaatctttt gcccatccaa ttgaatgtta tttaagactt
     7141 gaaaaacttg aaaagagatt ggcaaagcca taattatact caaaactaaa agatagacaa
     7201 tggctttcca aaatgggaca tttctcgcct cctttaggtc tttgaatcgt ataaaggaac
     7261 tgataattaa ttgttttgtt gtcataagta ctcctcatct agtagtgtca tcacttgcaa
     7321 ttctagcgaa gtatcaagtg aaatacaagt gctttaggga aacatctgaa tgattggtga
     7381 aatatcaaaa aaatgttatt cttgtctaaa gactatccaa tataaataaa atggaggaag
     7441 gaatagtgcc tacacagcca cagagatcat caaaaaaact atttggagta gtggttttga
     7501 atgcggtgat cactttatca gaatttattg gagggattat gtcagggagt ttgtctcttg
     7561 tttcagatgc tttccataac cttagtgata cattggcaat tattttttct tacggggcac
     7621 aaaaaatggc ccgaaaagaa gcaaataaaa aacgtacgta tggctaccag cgtttagaaa
     7681 tattgagtgc tttcatcaac tcattcatat tgatcatatt atccttgttt ttaaccgttg
     7741 aagcattcaa gcggttcact tcaccagaaa agataaatag tcatttgatg ctaacagtag
     7801 cagttattgg attgctcgcg aatttgttct cagtattatt attacgacaa gaagcagatg
     7861 aaagtttgaa tataaaatct agttatttgc atttgttaag tgatacactg tcttcggtaa
     7921 gtgtgattat cggagcaatt ttgatccgtt ttttcgatat ctactggatc gatccagtta
     7981 ttacactgat catttcgata tatatattga tagaagcgat aatggtgatc aaaaaagctg
     8041 ccgctatttt gatacaaagt gcacctgcta ttgactatga aaaaatggaa caagaaattg
     8101 aagcaatcga tggggtaaag aatgttcatc atgtccatat ttggcagtat agcgaaaaga
     8161 taattatttt cgacggacat attgattttg aagatcagct actaagcgag attgaaaaga
     8221 tatatcctcg tataatttct ttattaaaat taaaatatgg tattacacat gtgacgatcc
     8281 aagcagagac aaaggcaaag gatcaaaaaa aattaatttt tttaaataaa gatgaaagtt
     8341 attgaaatat agagtatgat aaataagaat ttttaataag gtaggttggc tcatgaagca
     8401 gtatcgacag ttaaaaggat atttagaagc aaaaggatat tgggagatac tagtctatct
     8461 cttttttggt ggtttggcta cgattgtaaa ttttgttagt tttgctctcg cacgccaata
     8521 ttttgattta agtatggcct tatccaattc tatttcgtgg ttttgttctg tattgtttgc
     8581 ttttgtaacg aacaaacttt gggtattcca ttcaaaatca ccgaatttta cacatgcact
     8641 tattgaatgc ggtaagttct tcttctaccg tatcctttct tacggattgg acatgggggc
     8701 aatggtattg ttgatcaatg ttatgaacag caatgagtat gtggcgaaaa tcatcactca
     8761 aattattgtt atcctagcga actatatttt cagcaagttg tttattttta aagaaactga
     8821 ggtttttgaa gaagaagtag gtcaaagtga aaaagacgac taggcataac aattgaagaa
     8881 tttccttctc tttgttactc tttgtaatgt agacattata aagaaaacat tgaggaggaa
     8941 tttttttatg acttacacat taccagatct accttatgca tatgacgcat tggaacctta
     9001 tattgacgaa gaaacgatgc atctgcatca tgataagcat cacaatactt atgtgacgaa
     9061 tttaaatgca gcaattgaga aatacccaga attaggcgaa aaaacaatag aagaattatt
     9121 atctgatatg gacgctattc caacagatat caagacagct gtacgtaaca atggtggcgg
     9181 acatgctaac cattcatttt tctgggaaat catggcacca aatgctggtg gcgaacctac
     9241 aggagaaata aaagaagcga ttaatgaagc ttttggtgat ttttcttctt ttaaagaaga
     9301 attcaaaaaa gcagccgctg gacgatttgg ttctggatgg gcttggcttg taatggaaaa
     9361 tggaaaatta gctattacct ctactgcaaa tcaagattct ccattgatgg aaggaaagac
     9421 accaattcta ggtttggacg tttgggaaca tgcttattat ttgaaatata aaaatgtccg
     9481 accagattat attgcagcat tctggaatgt tattaattgg gatgaagtaa acaaacgttt
     9541 cgaagcagca aaataaccat ttcctccgtc atatcagtct taaaaataat ttggaaaata
     9601 aaagagcgaa agcttgctag cgttttctaa attgggtata atgttcttgc aaggttactt
     9661 gcaagaactt ttttggaggc tatacattta tggaacacgg aacggttaaa tggttcaaca
     9721 atgaaaaagg atttgggttt attacagtag acggcggaga tgacgtattt gttcatttct
     9781 cagcaattca aggagacggc ttcaagagcc tagaagaagg tcaagaagta gaattcacga
     9841 ttgttgaagg tgcacgcgga cctcaagctg ctgaagtgac taaattataa taatttgttg
     9901 aataaagaca cgacttctta gagagtcgtg tctttttgtc tatttatgaa gtttttagat
     9961 tttttggcta agtaaatgca tttgttcact tgacatgtcc taactaatta tcaagcaagg
    10021 aacattttct tcgttagtaa tggattttaa agaaatttca gtttttggat gttgaataaa
    10081 agaccatttg tgaaaccact agtatacata ctcattacca tgtcaattct attactttaa
    10141 aagaaaatcg ttttactttg aagaggagca tatctatcca ttaaaagatg atggtaatga
    10201 aatcactagt tcgagtcatt ttaagagcca tgcagtgagc tggaaataaa acgaatagaa
    10261 gatgggttta acaaaaggac cagtcatagg aagaaatttt cgtgaaatcg aatgataatc
    10321 tgaacggtag gcaaagactg ttaaaaagat gggaaaggta tgtgcgatca ttttactgca
    10381 cgattatttg gaaaatacct tgttgttcgc tttttctgga aaaaatacgt aaaaaaagat
    10441 aaaacgattg cgaactgcgt gtattcggtc tttcgatctt tttcacggta tggtatgata
    10501 gttaagaatt ttgagaaatt ttaaaaaata gtaatcgtta cacaaaaatg ttttttaaag
    10561 ttttcttatg attaaaaagt gaatttgctg attgaacagt tatatgagac aatattttag
    10621 aaaatgtgaa acagaggtgt attatgaagc gatggcaaaa agttctatta accttgttag
    10681 gggtatttgt tttgctgatt gccggattat cagcatacgg aatcagattt atgggagaag
    10741 cgagccaaac agtaaacaaa atttctaaaa actctaatcg aatatcctcc aaacgttctg
    10801 agagagtaag cattgatgac aaagaacctt tttcggtttt gctattaggt ttagataccg
    10861 gtggattagg aagaacggaa caaggacgtt ctgatacgat gatggtagta acagttaatc
    10921 cgaaacaaaa aaaatcaacg atcatcagtt tggaccgaga tatttatacg aatatcgtag
    10981 gttatggaac ggtagataaa ttaaatcatg cctatgcatt tggtggtgtc gaaatggcca
    11041 tggattcaat tgagcaactg ctcgatattc ctattgatca ttatgtaacg atcaatttgg
    11101 atggcatgga agatttgatc aatgcggtcg gtggcatcaa agtgaataat aaaattgact
    11161 ttactttgga tggtgtccat gtaccagtag gtgaacaaac attgaatgga gaaaaaggtt
    11221 tagcctattc tcgaatgcga cacgaagatc cagaaggcga tatcggtcga caacgtcgtc
    11281 agcgggaagt agtgacgaaa atcgtcaata aagttttaag tttagatggc gtaagcaatt
    11341 atagaaaaat tttaaaagca gttgagaaca atgtgacgac ggacttagat tgggatgaca
    11401 tgcttgatgt agcgacgaac tacatccctg cgtttgaaac aatcaaacaa gaccagttgc
    11461 aaggcgaagg acaaatgatc aatgatatct actatcaaat tttaggaaaa aatgatcttc
    11521 tttcgatcca aaatgaactg aagaaacaat taaatataaa aacaagcgat acattaccta
    11581 atttgaaaaa tgagaatgct tcaatgatgt tctttgatga ttcggaagat ggcgattcga
    11641 caaacggtga tggatcgaat agtgaagggt acaccgatta ttcttcctat tcagaaaaca
    11701 catatgatca gtaccaagat ccgaatacct attatcagca aacagaagat acaagtcagt
    11761 actatacacc aagtcaagat actcaaagtt atgatcctta cgctggacag gaacaaaatg
    11821 gttatggtta tgaccagaat aatgagtatg gaacaacaag tgggtactag aaaaaaatag
    11881 tttggttaat aaagagtgcc acctaaatta gacaaaaaat ctaacttagg tggcactttt
    11941 tctatgtgtt aaattacagg acgtctcttt ttagcgctta gtttaaaaaa taaattgaat
    12001 aattcaatag agttgcgaat agtacccata aataataagg aatcatgagc cacattgcca
    12061 ggcgatgatg tagccacaac tgatacataa gcactgacaa gaaaataact aaaagtgtaa
    12121 tatcgattgc tgcaatgaaa atatttctta aattaaaaaa tagagcagac catataaaat
    12181 taaaaatgaa ctgaatcaca aaaagaccga caagattgtt tcgctgtttt ttgttgttgt
    12241 gattaagtaa caaataaaga gaaataccca tcaatatata cagtaaggtc cacatcggcc
    12301 caaatatcca gctaggtggt gcaaatggtg gaagttgcaa agaatagtaa tattctccgg
    12361 gatttccgct aagcaaccct gataaaaagc ctaatatgac aattcctata acacaaaccc
    12421 aaaaacgaat atcttttatc atcttcattt tactaactcc tttctataat agtagtttac
    12481 catgaaggct c
//
LOCUS       DAYYNG010000075        12422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000075 DAYYNG010000000
VERSION     DAYYNG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.075"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..243)
                     /locus_tag="ACHBFA_001702"
     CDS             complement(<1..243)
                     /locus_tag="ACHBFA_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9395189.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
     gene            complement(507..2453)
                     /locus_tag="ACHBFA_001703"
     CDS             complement(507..2453)
                     /locus_tag="ACHBFA_001703"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9395190.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            2638..4077
                     /locus_tag="ACHBFA_001704"
     CDS             2638..4077
                     /locus_tag="ACHBFA_001704"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9395191.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            4079..5041
                     /locus_tag="ACHBFA_001705"
     CDS             4079..5041
                     /locus_tag="ACHBFA_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9395192.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(5211..6637)
                     /locus_tag="ACHBFA_001706"
                     /pseudo
     CDS             complement(5211..6637)
                     /locus_tag="ACHBFA_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6880..7335
                     /gene="tnpA"
                     /locus_tag="ACHBFA_001707"
     CDS             6880..7335
                     /gene="tnpA"
                     /locus_tag="ACHBFA_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM9395193.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            7600..7698
                     /locus_tag="ACHBFA_001708"
                     /pseudo
     CDS             7600..7698
                     /locus_tag="ACHBFA_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(7921..8151)
                     /locus_tag="ACHBFA_001709"
     CDS             complement(7921..8151)
                     /locus_tag="ACHBFA_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM9395194.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(8381..9199)
                     /locus_tag="ACHBFA_001710"
     CDS             complement(8381..9199)
                     /locus_tag="ACHBFA_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9395195.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            9360..10049
                     /locus_tag="ACHBFA_001711"
     CDS             9360..10049
                     /locus_tag="ACHBFA_001711"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9395196.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            10063..11565
                     /locus_tag="ACHBFA_001712"
     CDS             10063..11565
                     /locus_tag="ACHBFA_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395197.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            11578..12054
                     /locus_tag="ACHBFA_001713"
     CDS             11578..12054
                     /locus_tag="ACHBFA_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9395198.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(12151..12261)
                     /locus_tag="ACHBFA_001714"
                     /pseudo
     CDS             complement(12151..12261)
                     /locus_tag="ACHBFA_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4115 a         2003 c         2187 g         4117 t
ORIGIN      
        1 tttcggggtc gaggtaatgt acccataagt ggcggaatcg cgatggatat cgataggccc
       61 aaatgagcca atcccaatcc ctgctaaatc tgcttggtat tgtttgaaga agtcaatgac
      121 taaaggcatt gtttcctcag gtgtcgttgt cggaaagctc actctttcct tgatcgtcat
      181 ttcttcatcg ccaatggcac acacaaattt tgtgccgcct gcttcaattg caccgtatag
      241 catagacatt tctcccttta ttttagaatg aaatcactaa ataaatgact atatatcata
      301 tttaaattat ttttttattt agtaacatga caagtaaata actactattt tttctttttg
      361 aatttttatc attttttaaa attctaataa tttttcatac aattgatata tgaaaagatt
      421 aaataaaatg ttattctcaa atatttgatt aatctatatt atttgatttt ggaaagaaaa
      481 ttccatcttt actcagggaa atatatttat ttgatttcaa gtaaatcagt accctcagtg
      541 actgtatttt cagctactat atttacttct gaataatccg aagtattggt cacaataatc
      601 attgttgtat catcaagacc ttctttctca atcattgaac ggtcaaatgc tccaagaata
      661 tcgccttttc taactttttg attagtagta actttttgtg taaatccttt tcctcccatt
      721 gatacagtat caataccaat atgaattaga acttcagcac cattatttga tttaatacca
      781 taagcatgtc cagtttcaaa cgtgagttga atagtaccat ctactggaga atggaccgtg
      841 tttccatttg gtttaatggc aatccctttt cccatagctt ctgatgaaaa gactggatca
      901 tttactgaag aaagactaat cactttacca gaaatgggac taacaattgt ttctgatgaa
      961 acttttgttt gtgttgattc aacatcagta gatattggat ctgttgttgc tacttttgaa
     1021 gaattaactg gtgtttgttc agcaatttct tctgctttaa agataccagc agcttttgtt
     1081 ttaccgtaga agtaagtaat tgcaaaagca actacaaagc taatcaattc acacaatacg
     1141 taaaatggaa tagatgttgc attaattgaa agaaatccca agaaacctgc tgaaccaagt
     1201 gaaacagcaa ctactttaag taatcctgct actgctgtag caattcctga accaattaaa
     1261 gcacaaaaga atggaaagcg gtattttaag ttaactccga acaaggcagg ctctgtaatt
     1321 ccaagaagtg ctgagacacc cgcagaggaa gcaagacttt ttgtcttaga atttttagtt
     1381 aagaaccaaa tggcaaatgt tgcagcacct tgagcaacgt ttgccataga tgcaactaca
     1441 aagataaagt ctccatgtcc gataccattt gtccacgctg aaagtaattg agtttcaata
     1501 gctggaaatg attgatgtaa accagtcatg actataagtg aatatgttcc tccaaaaata
     1561 cccattccaa taaatccagt agtgttatat agccaaacaa taatgtctgt aattccatta
     1621 gacaacaata acattacagg gccaataact gtgaaagtca aaaatccagt aatcattact
     1681 gataataatg gcgtgaatgt aaagtcaata gatgatggca attttttatg aaagaatttt
     1741 tctaatatag ataatatcca aacagctgct aaaactggaa taacttgata agcatagttt
     1801 gtttgtgaaa catgataacc aaaaatattc caatattcag ttgagccacc aagatctggt
     1861 gttgtcatga tcataccaat tgcagcacca agaaattggt ttgctccaaa acgtttagca
     1921 gctgaaatac ctactaaaat tggtaagaac atgaatggtg cagcggacat cagttggatc
     1981 attgaggaaa taccttcaat cgcaggatac atttcttcaa ttgattttgg tccaaaaagt
     2041 ccagcagctg ttaaaaagtt ttgtaatgcc attaataaac cacctgcaac aagggcaggt
     2101 ataattggaa caaaaatatc tgataataat ttaattaatg ccataatggg gttgaatttt
     2161 ttatctttac tagcaatttg ttttaaatca tctgttgaaa tttccgaaag tccagttttt
     2221 ttgataagtt cgtcataaac gaaattaaca tcacctgcac cgataatcac ttgatattgt
     2281 ccatcaattt taaatgtgct tttcacatct ggattttcgt ctaaagcttt ttgattcact
     2341 ttcgtgtcat cttttagtac gagacgaaga cgtgtagcgc agtgtgcagc tgcaataagg
     2401 ttatcttttc cgaccgcttg tattacgtcg gaagctactt tactgtaatt cattcgaatc
     2461 actccttttt tatttttacg tgaaaacggt tcactgaaat gcaaatttat tttagcatat
     2521 cttaaaaata tgtcaaacgt ttgtcatata gtttattttt ttaaatgaaa tgattgattt
     2581 ttcaacggaa aacgtttacc atactagttg taaaaagata aattgaagga gtaaaatatg
     2641 aacttacctc gagaaatacg atatcgtgct tattctgatt ggactaaaga tgagatggat
     2701 aaaattaagg ataatgtaaa acgatcacct tggcgtgcta gctatcatat cgaacctaaa
     2761 acaggattgt taaacgatcc taatggtttt tcttttttta acggaaaata tacgcttttt
     2821 tatcaaaatt ggccatttgg agctgctcat gggttgaaag aatgggtaca caatgaatca
     2881 gatgatttag ttcattttca tgtaacagga gcagaacttc ttccagacac aaaacgagat
     2941 agccacggcg cttattccgg ttctgcatat gaaatagagg ggaatctatt cttactctat
     3001 acaggaaacg ttcgaaatga aaattgggaa cgtgatacac ttcaaatcgg agcatggatg
     3061 gataaagact acaacattac gaaaggtgag aatgttctta ttcataaacc tgctgatgta
     3121 actgatcatt ttcgtgatcc acaaatgttt aattatgaag gacaattgta tgcgattgtt
     3181 ggagcacaaa atttagaaaa atcaggcttt atcaaacttt acaaagcgga tgacaataac
     3241 gtggaaacat gggaggaagt tggcaacctt gattttggcg gcactggttc agaatacatg
     3301 attgagtgtc caaatctagt atttgtagac aaaaaacctg ttctcattta ttgtcctcaa
     3361 gggttggata aatctgagct aaattatggg aatatctatc cgaatacttt taagatctgt
     3421 caatcgcttg acatagaaaa agtaaaactt gtgggcgctt cagaaattca aaatcttgat
     3481 tatggatttg aagcgtatgc gactcaagga ttcaatactc ctgatggtcg tgctctgatt
     3541 gtcagttgga ttggactacc tgatatcgat tatccaactg ataagtatga ttatcaggga
     3601 gcaatgagtc ttgtaaaaga actttcaata aaagatggta aactttacca atatccagta
     3661 gaagctatta catcattacg tgctagttct gaacaattta cagcaaaagc agaaacaaat
     3721 aatacttatg agcttgaact aacttttcct ccaaatcaga aatcggagtt acttcttttc
     3781 tctgatagta aaggaaatgg acttagcttg attgtagata ccaaagaagg aaaaattatt
     3841 ttagatcgca gcaaagcagg cattcaatat gccactgact ttggtacgat tcgtgaatgc
     3901 tcaattgata ctaaagaaac aactgctaac atttttgtag ataactcaat cattgagatt
     3961 tttattaata aaggagaaaa agtgtttact agtcgtgtat tccctgaaaa tcaccaaaat
     4021 ggtattcaaa tcaaatcagg cgaacctact ggaaaatact ttgaattaaa atattaaaat
     4081 ggtagcaaaa ttaactgatg tcgctgaatt agctggagtt agcccaacaa ccgtttcacg
     4141 cgtcattaat aataagggat atttatcaga aaaaacaaaa caaaaagttc atgaagctat
     4201 gaaaactctt ggttataaac caaataattt agcacgtgga cttcaaggaa agtcacctca
     4261 gcttattggt cttatttttc ctaatattag taatatcttt tactctgaac tcattgagta
     4321 tctcgaaata gagttattta aacatggata taaaacgatt atttgcaata gtcaaaatga
     4381 tcctgctaaa gagagagaat acattgaaat gcttgaagcc aaccaagttg atggcataat
     4441 ctcatcaagc cataatcttg gaattgctga ttatgagcgt gtgagagcac ctattgttgc
     4501 ttttgataga aatttagctc ctaatgtttc cattgtttct tcagataact ttgaaggggg
     4561 aaaacttgca gctaagactt tacaaaaaaa tggttgtcaa aataccatta tgattacagg
     4621 aaatgataac acggattctc ccacagaatt acgtgcacta ggtttttctt ttcaaaatcc
     4681 aaatggaaaa attttcaaaa ttcctaataa tctgtctact atccgtcgtg aaatggaaat
     4741 aaagtcaact attataaatc acaaaccaga tggtattttt gtttctgatg atttaacagc
     4801 cattctcact atgaaaattg ctcatcaact taaacttaat atcccagaag atttaaaaat
     4861 tattggatac gatggtacct cttttattga gaaatttttc ccacaattga caacaataag
     4921 acaaccaatt gatgaaattg caagtcttat tgttgatatt cttctgaaaa aaattaaagg
     4981 cgaaaagaca aataaggatt atattttacc aatctcaata ctttctggag gaagtattta
     5041 acgttaaatt aattttttaa tgtctataaa attcgttaga aaatacaatt tgtagatatc
     5101 caattgattt gtaaacaaaa aggagttgtc aacttgtagt gacccccaaa agttagactt
     5161 ttttggagtg gagaaatcca ctcctttttt catgctctaa aatgatattt ttacacaact
     5221 gttacggttg ttttacgaaa ttggacggga ctttgccagt ttaatttctg ttttatccgt
     5281 tcattattgt agtaatagat atagctatcg atggctgatt ttaattcctc gaaacagtta
     5341 taaactttcc cgtgaaaaat ctcttgtttt aataagccaa aaaaattctc cataagtgaa
     5401 ttatctaagc agttcccttt ccgagacatg ctttgaaaga ttttgtactc cttcaatttg
     5461 tttctataag ctttcatttg atacgcccaa ccttgatctg agtgaaacgt acgacgatag
     5521 ggacaatcgt tagtggtctg gatagcttcc tccaaacctt ccattaccgc taatgcattg
     5581 ggcttttcag agatacgata ggataaaatc tcactattga acatatccat aaaaggatct
     5641 agatacaatt tcttttgacg gataatgcct ttggcatcca cttcatggta tttaaattca
     5701 gttgtatccg tcgtgatttt ctgatggcag atactggtgt agaaacgacg atggatccga
     5761 tttttggcta ctgctccgac ttgtcctcga taagaattgt agcgccgtga tttacgtgtg
     5821 tatgtcgtta cttttattcc tagcttcttg atcaggcgct gaactttctt tttattgata
     5881 gagaaaccgc ggtttcttag ttcattggtc atccgtaaac agccataatc cttgtgcttc
     5941 tcccgaactt taagcatttc cgcttcaatc tcttgatctg gatttttacg gtccaatcgt
     6001 ttttgccagt acataaagat tgccttaggg aactttaaag tttctagaag ctcaactaat
     6061 ttaaacggtc ctcggaggct tgcgatgatt cgtgcgattg cttcattcgt cgttgttggg
     6121 cttcctgttt tcgcagcttc ctcaattctt ttaaaaaggc attttcaatt tgaagggaac
     6181 ggactcgttt ctccaactcc tcgatgcgtt cacgttcttt agacgttgct ttagttgtag
     6241 attttttctt atttttgggt tcatttttct tagacaagat aggtggacat cctttcgttt
     6301 ttgagagtcc atcgatccca ccctcacgga attgacgcat ccaactagcg atcaaattag
     6361 ggttgttcat gttaagtgtg ttggcaactt cacgatagga cagctcactt gttagatata
     6421 actctatcgc atccagctta aattgaacag aatatttttt cttttgtcgc tttcttacta
     6481 gaccctcttc accaaattct tgataggcat tgatccagcg cttcacttgt gatggagcct
     6541 taatggaata tttctttgcc agataatcac ttccgccttt accatccaag taatcatgaa
     6601 cgatctttaa tttaagttca aaagtatact tagccataaa aaataccgac ctccaaaagt
     6661 tagatttttt ggtctaactt ttgggggtcg gtacaaaact taaaagtttt agaccctttt
     6721 attttcttaa tgacttcagt cagttttgtg cgtaaagcgt gcgaaacaag aaaaccgcgc
     6781 gtttctacgc gtggaagaag tatctttagc gataaaaact cctttcgata tactagtgag
     6841 tggctaccaa ccgctcatca gatcgaaagg agttttttca tgtcaaatga cgataaaagt
     6901 ttagcatata ctcgttggaa ttgtaagtat catattattt ttacaccaaa atatcaacga
     6961 aaagtaatct atgggcaatt acgaaaagat cttggaaaaa tacttaggaa gttgtgcgaa
     7021 atgaaagatg tggaaatcat agaagcacat gcaatgctag atcatattca catgcttgtt
     7081 cgagtaccac caaaaatatg tatatccagt tttatggggt atctgaaagg tcgtagtgcg
     7141 gtactaatcc acgagcagca cgcaaatctc aaatataaat acggaaacaa atccttttgg
     7201 tcgaagagat actatgtgag tacagtcgga ttgaatcaaa agacaaatga aaaatacatc
     7261 cgtgaacaag aagctgatga tcgcattcga tatattataa gtaaaagaga gtacgtagaa
     7321 ccatttaaaa aataaagaga agtaagtgag tgaagatgag cagttggcgg tcttttaaaa
     7381 atcccctttt aaggggcagc gagtgtcgac cccttctagg gggattaaaa atacacccgt
     7441 cctcacgggt gtatgggtta ctgatagact tgattttaac tggtgtcaga aacttatgtc
     7501 cagaaagggt gatgaaattt tggcaattta cggctattca agaattagca caagcaacca
     7561 agattacaaa acacaaattc aaacattaga aaatgctggc gcagaaataa tcttcctaga
     7621 aaatcactgg attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat
     7681 ctgtctaact taatttgaca attaagagta tatttacttc agatggtaag gcaacaaaat
     7741 aaaatacaat aggcagatga actaataaat aaaatctgta ttaaatacag ataaattaat
     7801 ataaaaatca aaaggatcat aaacttttaa caagtgatga aatgggtgga aattgattct
     7861 tatagacagt ttgtctcata acattgaagt attttgcaat tttagcattg aaacactttt
     7921 ttaatcttca acgttgttct tataaacaag atgattggga atcttttgaa ttaacactgt
     7981 atagcttaga acacccttta tagaaaacat tcgcttagca atcttaataa ttccttgtgc
     8041 ccgatgtcgt ttagattcag taggttcctg ctcatcgatc atggaaaaaa tttgaaggat
     8101 cagatcctta ataaatttat ttaagagagg atgatccatt gcttcatcca tactcgataa
     8161 actagtgata atgagctaca cgtttttctt tcaagttgat caatagcttc cacgataaaa
     8221 atatctttag ataaaatcaa gaaccgtagg aactacaggg atatttggtc aataagagcc
     8281 accgctatta gtaaaaaata agctatcaag tatgctctca tcccaagaat tgcctacttc
     8341 aatcgttaaa aaccgttagg tttagctaag tgggggtagt tcactcaata cttttatcaa
     8401 taatagagcg caaaactttt tctcttaatt caactgaatc aatattattg ttttgttcat
     8461 aaccatgaac taatagatca caaatataaa gttgtgatat ttttccagct aaagatccac
     8521 cattaagaaa ttcttcaatg gctgtttgta aaactaagtc tgcagacttt cctataggtg
     8581 aatgaatata gttagttata gcaataattt ttgcgccatt ttttttagct attttcaatg
     8641 aatcataagt atctttcgtt ttcccagata aagaaaaaat gattactaaa tcattaactg
     8701 ttaataaaga agcgacttgg gcttgaaaat gagggtctag tacagctttt gcttgaactc
     8761 ctacgcgaag aaacatattt tctaaatcaa gactagtatt tccactagat cccactccaa
     8821 atatatatat gttttttgat tgagtgatca ttttaatggc ttcattcaat ttttcttcgt
     8881 ctaaaagcct aatagtggaa tagagagctt ctgttaaact attagcaatt tcatcatagt
     8941 actttacact agtcggatgt tctttttttt gactaaagtc ttcctttgca atttcaattt
     9001 ttaaatcaga aaaaccagaa aaaccaattt tttgacaaaa gcgaataata gtagcatcgc
     9061 caacttttgt gtttttctta atatcactca tcgtactata aataatagtt ttcccagaat
     9121 ttaaaataaa atcagcgact tttttttcag acttcgttaa aaatggataa ttttctttaa
     9181 ttaaatagat ataactcata caaactcctc tacaatttca acttttctcc attatactcc
     9241 aaaataataa aaaaaactcc taaatactcc atttttatct ttacttttat gaaagcggat
     9301 gctatattgt ttttggagtt gttatccaaa aataataaaa aattggagtg tttaggataa
     9361 tggataaaaa gaggttttta gatcttattt caaaaggttt gattgtttcc tgtcaagcat
     9421 tacctggtga accattttac aaagaggaag aaagtttaat gccttatttt gctttggctg
     9481 ctcaaagggc tggagcagtt ggtatacgtg caaattctgt tcgagatatt attgaaatta
     9541 gaaagtgtgt atcattacca atgattggaa ttattaaaag agattatcca ccagaatctc
     9601 cattcattac tgccactatg agagaagtag atgaattagt agaaaccggt gttgaggtaa
     9661 tcgctcttga ttgcactctt agaaatcgac atgatggact atctataaat tcttttatta
     9721 ctaaaataaa agaaaaatat cctcatcagt tatttatggc agatatttct acatttgaag
     9781 aaggcaaaaa tgcatataaa gcaggaatag attttgtagg aactacttta agtggatata
     9841 ctgaggaaag taaaaaacaa gatgggccag atatagattt gattaaaaaa ttggtagaag
     9901 aagggatttg tgtaattgct gagggaagga ttcatactcc agaacaagca aaagagataa
     9961 aaaaaatagg agttgcaggt attgttgtgg gtggtgctat tactcgacca caggaaattg
    10021 catcaagatt tgttaaagca ttaagttagg gggagaaata aaatgtttaa aaaattttca
    10081 caattaggac gcgctttcat gttaccaatt gctatattac cagtagccgg attactattg
    10141 ggactgggag gagcgttaac aaatgagagt gctataaatg cttatccatt tttagatcaa
    10201 ccgtggttgc atacaatttt aagtattatg agttatgctg gaaatgcagt ttttgcaaac
    10261 ctagcactaa tttttgcaat tggtattgct gtaggacttg caaatggaga taaaggaaca
    10321 gctggtttag ctggtggagt agcttatcta gtatatactg caactattag tgggtttctt
    10381 gaactttttt cagcaaaaga tgcaacacta gatacaggtg tagtgggatc aattgttgtt
    10441 ggtggtactg ttgcattttt acacaatcgt tatagaaaaa ttgaattacc acaattctta
    10501 ggtttttttg gaggatcaag atttattccg attgtttcta gtttagctgc aatcattatt
    10561 ggttctttct tttatttaat ttggccacca attcaaaatg gtttagtagt tgctggtgaa
    10621 caaattgccc aaatgggtag tttaggaact tttttatatg gttttttatt gcgtttaaca
    10681 ggtgctgttg gactacacca caccatttac cctttgttct ggtatacatc tttaggtggt
    10741 accgaaactg tagctggatc aacaattgct ggagcccaaa atattttctt tgcccaacta
    10801 gctgatccaa accacactgg tctgtttact tatggtacta gattttttgc aggaagattt
    10861 gcaacaatga tgtttggatt accagcagca tgttatgcaa tgtatcgtgc aattcctaag
    10921 aaaaatcgta aaaagaatgg tgggctttat tttagcgggg cccttacatc atttttaact
    10981 ggaattactg aaccagttga atatatgttt ttatttgttg ctccttggct ttatgtgatt
    11041 catgcattct tagatggatt atctttttat tttgctgata tcttaaatat tcgtgtaggg
    11101 aattcctttt caggtggttt gatcgacttt ttattattcg gtgtacttca agggaatgac
    11161 aaaactaatt ggttaaaatt aattcctttt ggtattgcat gggcatttat atatttcttt
    11221 gtttttacat tctttatcaa aaaatttaag gtagctattc ctggaatgga agaagaatca
    11281 gaagttcatt ctgaaaaagt aataaacaaa aatacatcat tacatgaaga gtctcttatc
    11341 attattgaag ctcttggtgg aaaagaaaat attgagtctg ttgaagcttg tgcaactcgt
    11401 ttgcgtgttg ctgttacgaa tggggagatt attcaaaaac aaacaataaa agacactggt
    11461 gcaactgctg tcttcgaagt gaagggtggc atccaagctg tttttggagg gaaagctgac
    11521 ttattaagtc aagaaatcaa tcaaatatta ggaaaagata attaggaggt ttttaatatg
    11581 tttgaaattt tcaaaaagaa aaaacaagaa actttttatg caccttgttc cggaaaaatc
    11641 atttccatca cagaagtaaa tgatcctgtc ttttcacaaa aattactagg tgatggtttt
    11701 gccgtcatac ctgatggttc aaaaatttat gctccattgg ctggtcgagt aacaaatata
    11761 tttcctacta aacatgctat taatataaaa agcaactcag gcattgatta tttgattcat
    11821 attggaattg atacagttga attacaagga gagccttttt cgatttgtgt tcaagaaaat
    11881 gatgaggtta ctcctgaaac ctcgttggtt gatgtggatt ttgacaaaat aataaaatct
    11941 ggtaaagata cttctgttat agttgttttt ccggagaaaa atcagttcaa tgatttaata
    12001 atagataatg gtcgaattgc tcatgggaat tcatgtggaa aaatcacttt ttaaaaaagt
    12061 gtatataaaa agctataaaa taagctaaat aggatatttg tcaaatgaag ttggtgaaca
    12121 gaaagaactg ttttctttct gttcattttt ttattttatt agtaatgatt ccccatgcca
    12181 aatcatgatc cgtaatttaa agttataaaa gcttcgaaat ccattggcat ttcgttggaa
    12241 taccttgata tgattattca aatataaaat atagagagaa gagagagagg gaagatattt
    12301 ataaagggca aagttatgtt tactcaactt aggaaataga aatagatcaa tttatattct
    12361 cttgtcaatc gaatatgaac cttagcacta tataccaaat atagtaaaat gctgaaagat
    12421 gt
//
LOCUS       DAYYNG010000076        12398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000076 DAYYNG010000000
VERSION     DAYYNG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.076"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1859
                     /locus_tag="ACHBFA_001715"
     CDS             <1..1859
                     /locus_tag="ACHBFA_001715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9395199.1"
                     /translation="SRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGVPIFFVVSGY
                     LITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQRGLLNNLRGV
                     VVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPILFVLLMVFVR
                     KRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSALAFVWPSTR
                     LKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLLCVVLVAVTA
                     HPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLLHTLVEITLI
                     LIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLLVAIALVGFV
                     TAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYELSKAQGNKAQ
                     KLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQDLLKDTVLI
                     GLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMTKKYDNMTLI
                     DWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETNVTE"
     gene            complement(1934..2218)
                     /locus_tag="ACHBFA_001716"
     CDS             complement(1934..2218)
                     /locus_tag="ACHBFA_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9395200.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2208..2942)
                     /locus_tag="ACHBFA_001717"
     CDS             complement(2208..2942)
                     /locus_tag="ACHBFA_001717"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9395201.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3071..3694
                     /locus_tag="ACHBFA_001718"
     CDS             3071..3694
                     /locus_tag="ACHBFA_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9395202.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3886..5007
                     /locus_tag="ACHBFA_001719"
     CDS             3886..5007
                     /locus_tag="ACHBFA_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9395203.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5014..8142
                     /locus_tag="ACHBFA_001720"
     CDS             5014..8142
                     /locus_tag="ACHBFA_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9395204.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8161..11355
                     /locus_tag="ACHBFA_001721"
     CDS             8161..11355
                     /locus_tag="ACHBFA_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM9395205.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11501..12208)
                     /locus_tag="ACHBFA_001722"
     CDS             complement(11501..12208)
                     /locus_tag="ACHBFA_001722"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9395206.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4209 a         2199 c         2621 g         3369 t
ORIGIN      
        1 acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg ggtgttatct
       61 tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg atttttttcg
      121 tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa aatggaaaaa
      181 tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca cttgttttta
      241 tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta aacaatcttc
      301 gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc aataacggtt
      361 tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg tctttagcag
      421 tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg gtatttgttc
      481 gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc gccatcttga
      541 tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt accgacactc
      601 gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa
      661 agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga atctcgcttt
      721 tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc tattacggag
      781 gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca caccccggag
      841 caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa cgaagctatg
      901 gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg actgatattg
      961 ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc atcagtgaac
     1021 tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt acattccaag
     1081 tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg gttgttccag
     1141 gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa
     1201 cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa
     1261 ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt gataagtacg
     1321 agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca tttggtgatt
     1381 cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta gtggtagatg
     1441 gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta aaggaacaag
     1501 atttgttgaa agacactgtt ctgatcggac taggaacgaa tggctcattc tcggaagctc
     1561 agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc aatgtccgag
     1621 taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg acgaaaaaat
     1681 acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt gactggtttt
     1741 atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac ttagtcagcg
     1801 aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg acagaatgat
     1861 agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt tatatttacc
     1921 gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac
     1981 gttctttttg ttttcgggtg agttttttga atgcagcttc tgcttttgtt gcttcgcttc
     2041 ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga agacgagtat
     2101 atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg gttgtatatc
     2161 caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca tttgccatag
     2221 agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat caatggtgga
     2281 gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa aatatttgcc
     2341 tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg atagtcttcc
     2401 atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa gcgtccattt
     2461 gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg aatctcatga
     2521 cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc tttgaaataa
     2581 ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc
     2641 agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat actacgtctt
     2701 gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg ggttcgttta
     2761 cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt ccctttttta
     2821 ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa cactttggaa
     2881 ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc
     2941 atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt tattaaaaag
     3001 aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata agaagggaaa
     3061 ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat tagccttagt
     3121 taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga attacatact
     3181 agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg cagcacccaa
     3241 ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc gatttatatt
     3301 aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg tcattaaaac
     3361 acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca gtgggacaag
     3421 acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta aagcgaaaat
     3481 cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc gtcagagagt
     3541 gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg ataaagaagg
     3601 tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca aagaagtgaa
     3661 ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc
     3721 ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg gagagacgtc
     3781 aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc atgtaatgga
     3841 tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg ttttttacat
     3901 acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga agaccagcgt
     3961 catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt
     4021 gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt atacaatgaa
     4081 ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc aggaaatcac
     4141 gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa ctattttttg
     4201 cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca atttttcttt
     4261 cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa tatccgaacg
     4321 atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga ttcagaaaaa
     4381 aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga ttcggaaacg
     4441 aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc gtttgattat
     4501 gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc tcgctatagc
     4561 ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt
     4621 gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca tgatattgaa
     4681 gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc cgtcaatcgg
     4741 gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat gatgaaccag
     4801 cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg agcagaacaa
     4861 acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc agaagcattt
     4921 tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct cggagaaacg
     4981 ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa
     5041 aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga agcaccgctt
     5101 tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg gatcaccttc
     5161 gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg ttctttgttt
     5221 gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca gatgagctat
     5281 gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa
     5341 caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat aaaacaaatc
     5401 acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga tgcgaaacag
     5461 ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt ttcatcaagc
     5521 agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca acagttcaat
     5581 gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt agagcaagag
     5641 ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc agcagggttt
     5701 gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca aatcacggaa
     5761 gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa ttcctactat
     5821 cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa caagcaaaag
     5881 gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg gtattatgaa
     5941 tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt tgaaaaaata
     6001 gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac gcagaattat
     6061 gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat caaagaagct
     6121 gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt acgtagtaaa
     6181 aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa tgccgagaag
     6241 ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga aaatttactg
     6301 gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata tctagatgaa
     6361 aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc acagataaga
     6421 gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt gaaagaacag
     6481 acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag
     6541 ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca ccccatacaa
     6601 caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca acaactggca
     6661 gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga agcacatgaa
     6721 aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac gaatatacaa
     6781 ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc tgagataaca
     6841 gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag acgaaaaagc
     6901 aagatggcca atgcaagaat agagctggaa gaaataagac aacaagcaga aaagctcgaa
     6961 caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct ccagcaactg
     7021 caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa ctgtttagaa
     7081 gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact agctgttgag
     7141 cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca gcttgatctt
     7201 caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga aagaatagct
     7261 aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc atttgcacaa
     7321 cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga agagcaatta
     7381 gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac attcccagat
     7441 acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat aaagcaagaa
     7501 aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt
     7561 tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca gcagctttcg
     7621 caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt gcttcaggtt
     7681 tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt
     7741 tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg attagaaatc
     7801 aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag
     7861 agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca gacacaagct
     7921 ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt agatgaagaa
     7981 gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag aatgatcggc
     8041 atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag aattgataca
     8101 gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag attggtaact
     8161 atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc
     8221 cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc
     8281 agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta ctgccaatgt
     8341 ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact ttatttacgg
     8401 tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca
     8461 acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct
     8521 caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac taatcagtat
     8581 cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc atcaccgaaa
     8641 aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga aaaagaaact
     8701 tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca aaaagaagat
     8761 caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt aggtacgaac
     8821 ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt agaacaagcc
     8881 cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat aaatgaacaa
     8941 aacgatcaag gaatacaatt ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa
     9001 tttggagaag attatttgct gcaagcattg aatgattttg attttttctt tatgcattat
     9061 cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca aacggtttat
     9121 caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa
     9181 gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg cgacgtagct
     9241 attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat tttctttttc
     9301 cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggcga agtcatctat
     9361 tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca
     9421 gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa aaatgaaact
     9481 ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg cacagaacta
     9541 tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga gttgtattta
     9601 gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt
     9661 cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca aagcttgctg
     9721 agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga ttcagaagaa
     9781 tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac agaagaagga
     9841 acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga aaaaagcaat
     9901 gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc agaaagatac
     9961 caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa gcaaggtttt
    10021 cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga tgaaatggga
    10081 cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga acggaaaagc
    10141 ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc agaggtgaga
    10201 aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga acgtgaagaa
    10261 ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag acaggatcta
    10321 gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca aatggtcaaa
    10381 aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca gcggtttgca
    10441 ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt tgcaacgatt
    10501 atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg
    10561 caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa agacttgccg
    10621 gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac tgtttattta
    10681 gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc attcaagaaa
    10741 aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg cgatcctcgt
    10801 ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa agatctactg
    10861 gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac agggatgctg
    10921 actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt aagaggcagt
    10981 acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga aaaagatgtt
    11041 tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc agatacggtc
    11101 atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg acgtgcacat
    11161 cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg aacgattgag
    11221 gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt
    11281 aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt aagtgtagga
    11341 gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa
    11401 agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc attttatccc
    11461 agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag attgtcttca
    11521 ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa
    11581 ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt gcacctagaa
    11641 catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata tgaaattctt
    11701 tctcagctag ctgaaaaatc tctttttctg gtttttggaa acctgttgct tgagaaatga
    11761 gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt tttgtctgat
    11821 ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc aagaaatcga
    11881 gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg taaatttttt
    11941 gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt agtgattggc
    12001 tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact tttgggaagt
    12061 tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca tctgaaacaa
    12121 gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac atcgtatcat
    12181 caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt tcattgattt
    12241 tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg ataaataaag
    12301 gtttttctta ctatatttct atttccttga cagaataagg tttttatatt tcagtttttt
    12361 tcactaaaaa tcgtgaaaca cgtcaataag tttgatga
//
LOCUS       DAYYNG010000077        12338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000077 DAYYNG010000000
VERSION     DAYYNG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.077"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACHBFA_001723"
     CDS             <1..154
                     /locus_tag="ACHBFA_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM9395207.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACHBFA_001724"
     CDS             complement(208..486)
                     /locus_tag="ACHBFA_001724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395208.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACHBFA_001725"
     CDS             complement(601..1218)
                     /locus_tag="ACHBFA_001725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395209.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACHBFA_001726"
     CDS             complement(1613..2437)
                     /locus_tag="ACHBFA_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9395210.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACHBFA_001727"
     CDS             2780..3196
                     /locus_tag="ACHBFA_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395211.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACHBFA_001728"
     CDS             3193..3663
                     /locus_tag="ACHBFA_001728"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395212.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACHBFA_001729"
     CDS             3677..4450
                     /locus_tag="ACHBFA_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9395213.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACHBFA_001730"
     CDS             4464..5276
                     /locus_tag="ACHBFA_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9395214.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACHBFA_001731"
     CDS             5330..6295
                     /locus_tag="ACHBFA_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9395215.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="ACHBFA_001732"
     CDS             6432..9155
                     /locus_tag="ACHBFA_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9395216.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="ACHBFA_001733"
     CDS             9168..9800
                     /locus_tag="ACHBFA_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9395217.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10008..10232
                     /locus_tag="ACHBFA_001734"
     CDS             10008..10232
                     /locus_tag="ACHBFA_001734"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM9395218.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10334..10567
                     /gene="relB"
                     /locus_tag="ACHBFA_001735"
     CDS             10334..10567
                     /gene="relB"
                     /locus_tag="ACHBFA_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9395219.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="ACHBFA_001736"
     CDS             10557..10826
                     /locus_tag="ACHBFA_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9395220.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11005..12306)
                     /locus_tag="ACHBFA_001737"
     CDS             complement(11005..12306)
                     /locus_tag="ACHBFA_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9395221.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4031 a         1998 c         2422 g         3887 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg ctctttgtca aataggactg atagagtgag ataagaacat
    10981 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    11041 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    11101 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    11161 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    11221 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    11281 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    11341 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    11401 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    11461 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    11521 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    11581 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    11641 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    11701 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    11761 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    11821 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    11881 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    11941 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    12001 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    12061 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    12121 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    12181 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    12241 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat gatgaagatc
    12301 aggcatacag aacgctcctt tgattgtgtt gtggtgat
//
LOCUS       DAYYNG010000078        12082 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000078 DAYYNG010000000
VERSION     DAYYNG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.078"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..750)
                     /locus_tag="ACHBFA_001738"
     CDS             complement(97..750)
                     /locus_tag="ACHBFA_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9395222.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1038..1558
                     /locus_tag="ACHBFA_001739"
                     /pseudo
     CDS             1038..1558
                     /locus_tag="ACHBFA_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1556..2275)
                     /locus_tag="ACHBFA_001740"
                     /pseudo
     CDS             complement(1556..2275)
                     /locus_tag="ACHBFA_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2300..2485)
                     /locus_tag="ACHBFA_001741"
     CDS             complement(2300..2485)
                     /locus_tag="ACHBFA_001741"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM9395223.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2712..3985
                     /locus_tag="ACHBFA_001742"
                     /pseudo
     CDS             2712..3985
                     /locus_tag="ACHBFA_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4092..5123)
                     /locus_tag="ACHBFA_001743"
     CDS             complement(4092..5123)
                     /locus_tag="ACHBFA_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395224.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5130..5960)
                     /locus_tag="ACHBFA_001744"
     CDS             complement(5130..5960)
                     /locus_tag="ACHBFA_001744"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395225.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5957..6772)
                     /locus_tag="ACHBFA_001745"
     CDS             complement(5957..6772)
                     /locus_tag="ACHBFA_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395226.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6769..7593)
                     /locus_tag="ACHBFA_001746"
     CDS             complement(6769..7593)
                     /locus_tag="ACHBFA_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFM9395227.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7583..8683)
                     /locus_tag="ACHBFA_001747"
     CDS             complement(7583..8683)
                     /locus_tag="ACHBFA_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9395228.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8861..9646
                     /locus_tag="ACHBFA_001748"
     CDS             8861..9646
                     /locus_tag="ACHBFA_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM9395229.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9679..10062
                     /locus_tag="ACHBFA_001749"
     CDS             9679..10062
                     /locus_tag="ACHBFA_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9395230.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10138..10269
                     /locus_tag="ACHBFA_001750"
     CDS             10138..10269
                     /locus_tag="ACHBFA_001750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9395231.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10238..10474
                     /locus_tag="ACHBFA_001751"
     CDS             10238..10474
                     /locus_tag="ACHBFA_001751"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9395232.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10486..10572
                     /locus_tag="ACHBFA_001752"
                     /pseudo
     CDS             10486..10572
                     /locus_tag="ACHBFA_001752"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10552..11523)
                     /locus_tag="ACHBFA_001753"
     CDS             complement(10552..11523)
                     /locus_tag="ACHBFA_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM9395233.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3633 a         2738 c         2214 g         3497 t
ORIGIN      
        1 gaaagataaa aaaaattaat gaatgaacta atataattat tattccaaga acagaaaata
       61 tttgtatgaa catttttgga aatatcttta attttttcat tttctctcca atttatatcc
      121 tacatttctt atagtagtta tacagtctaa ttgtaatttt tttctaagtt cttttatata
      181 tacatctatt actcgatcat aaggagtttc ttcgctatct ttccatacat aatcaataat
      241 ttgcattctt gttaacactt gtccattatt atccaataaa cattttaata cttctagttc
      301 ttttgcattt acatctattt cttcaccatt tattttagct gtatagctat taaaattaac
      361 cgatagatcc ttatattcaa acttctctaa atgtccataa ttcttcttaa ttaaagaatc
      421 tattctagct tttaagaccg gcaatgaaaa tggcttttct acatatccgt ctactaaatt
      481 agtaaatgca tcaattttat attcttcatc actaaatgca gtcaagatta gaattggtaa
      541 attgcttttc tttctaattt ctttcaacac ttctaaacca tttataaaag gtatctgaat
      601 gtctaagata accaaattta tatcgctatt aaattttgac aaggcttctc ttccatcttt
      661 agcttgaata acagtatatc caaattctga aagatattca cttattccct ctcttatcat
      721 attatcatct tcaacagtta atattttcat atatacctcc acttaagcct atacactaga
      781 tattatacag catattatgg ctctataata tctagtgttg gtgacgcctt ctgttgccaa
      841 tactttttct tttttttaat aaatgcaaac agacaccttg aaatccttta gaaaaaaggc
      901 ttctataata ttttgtgttg ttggaaaatc gaaggcgatt ttctactaac actttttctt
      961 cgtttcaact caaaaagaga ctcacttttg gtaaaattta gtcgaccaaa acatcaccct
     1021 accaaaggag tcgctctatg gataatcata ctagaaaatt actcggatta acagataaac
     1081 atttattttt tgatgaagaa tggctgattg aaaaagaata taaaggcgtt caagcgcagt
     1141 ttattaaagg gagattggat gactcacctt cccattgtga acactgtgga tcgattcgta
     1201 ttatccgcaa cggttcctat acgacacgta ctcagatgtt gaaagtcaaa gaaaagttga
     1261 ctattttaga attaaaacgt acccgttttc tatgctatga ttgtgggcag accttttctg
     1321 cgaaaacgga cttagttgat gaacatcatc aactaacaaa ggaactgaag caggcgattc
     1381 tcatagaact gtacgaaaat caatcacgta atctgaaaat cagaagaagt accgtcggct
     1441 caagcgatat tggaaactct tactcaaaga ttccacgaca ttggagccgc tcaaacggca
     1501 ttatcatcga ctgtttaaac gaccaattag tcaaacagaa atcgtcgatg aaccgttgtg
     1561 cacaatggtg tcctgaacgt acagcgacac cttccatatc cagagcagtc gccacatcac
     1621 ggggatggat gccatccaaa ttaaaagcaa ccacgccagt atgatgttca ggatcttgcg
     1681 gtccgtaaat ggtgataccg ccgatcgctt gtaatttagg taaaacgtag gcaaccagtt
     1741 cttgctcata acgatgaata ttctccatac caatctcatt caagtaatcg attgctcctg
     1801 caatgttcgg agtaccagct tcaaatttcc atggcagctc tttccatgaa ctatcgtaaa
     1861 gattgacaaa atctatcatt tcaccgccga attcgactgg ctccatttgt tctaacaatt
     1921 gacgtttgcc atataataca ccgattcctg tcggcccgca cattttatgc ccgctaaagg
     1981 cgaaaaaatc tgcatccaac gcttgtacat caactgtcat atgcggtgct gcttgggcac
     2041 cgactggact atcctcaact acaacagcct gttcaaccct cagccgtttc aagcacaaca
     2101 aataaccttc tggatctggt ttgccgacct gaagatcatc gatcgtcaca aaggaatcaa
     2161 ttttttcctt aatcatcgga tgtgcttcaa aaaaatagtc tagctcccgc agttcagaat
     2221 tgagactact ccgattttcc agtgctgctg atccgctgct caaaatgttc agcatccact
     2281 ggtctcccaa ccgcttgact tatcccttca taaaaggaca gttctgctgc cgtcccaaca
     2341 ccttgttgat agtctgcaaa aggaatttta tagccgaact gctccagtaa ataggcttca
     2401 gtaaatagtg atgaatactc tcgtaaaaca tccattcact gtcaatcatc gtcccatcaa
     2461 aatcaaaaat aagtgctttg gtcatcttct ttccccctta cccaaaacaa gcaaaagctg
     2521 tgtcctgctc tctctacagt atcagcataa aaactgccgt ctgaaaagtc aaaggtgaaa
     2581 gaaatgtggc gctataattt ctaggactga tagtaaatca ttctatcagt ccttttgagc
     2641 ataaaaaaac aaaacataca gaagctccgt tagaattgaa tcaccacaac acaatcaaag
     2701 gagcgttctg tatgcctgat cttcatctta tcaaactatt actaggaatc aaagatggaa
     2761 acattcaaat cactcatgtc gagaagaaaa aaatacattc aatcaacagt atcgttgttt
     2821 ctgccacgct cattaaaacg attcagccct gtcctcagtg taaacaggtg aatcaacatg
     2881 ggctgatcgt taagaacgga aagaaaaaat cgctggtcct tctcaataag tgtgcgaatc
     2941 aacggactta tcttgctttg gcaaagcaac ggtacctctg tcgtggctgt cacacctatt
     3001 ttacggcgaa tacctacatt gttgaaaaaa actgttttat cgccaaacaa gtacattata
     3061 aaatattgga ggaattgact gaaaaacaag caatgacgac gatttctaaa cattgcggcg
     3121 tttcttggtc aaccgtttcg cgaacactcg cttctctgct ccccatgaca aacgtgaaaa
     3181 taaattggtt gcctcgctgt tcattggtag atgaatttcg ttcattgaaa aatcaagtcg
     3241 gtccctattc tttttcttgt atggatggcg atactgggaa attactggat attcttcctt
     3301 cacgtaagaa gaaagatctc atttcttatt ttatgcaatt tgaacgacgt gcacgtttaa
     3361 acgtaaaaat actcgtaaca gatatgaacg cctcttatgc tttgctgata aaagagtgct
     3421 ttcctcatgc caaactcgtt gtggatcgct ttcacatcgt caagcacctc attcgcagtt
     3481 ttaaggatat ttgactatga gtcatgaaaa gcttcgaccg aaacgatcca atacaagcca
     3541 aacactatcg acaagtgaag gctttatctc gacttctgat caaacgacag gacacgcttg
     3601 tttatgataa atggacaaaa tggcgtaatt ttggttgggc ctatttaacc gaaagtgaag
     3661 tcgtagaacg tctactttct acttctgatg aactacgaat agcttatgct tactatcaag
     3721 aaattcttca agctttttat gacaaagaag cggacatctt ctttcagctt gtaaaaacaa
     3781 tgcccaagtc ggttccaaga gaactgcatc atataaaaaa agcgtttata aactatgaat
     3841 ccggcattcg tttagcctta gagttgcctt actcaaatgg caaaatagaa aatctgcata
     3901 cccacatcaa agcattgaaa cgaatagctt acgggttagg atgagaacca gaattttctt
     3961 aatcaatcat ctgatcactt attaaacata aaaaaacatc taaagcagaa aatcaattct
     4021 accttagatg ttcttatatc actctatcag tcctatttga caaagagcca gaaatgtttg
     4081 ccgactatac actagctttg ttgtgcaaaa tgctgagtat caatttccac agggattgct
     4141 agaatatctt tcagctgaat gtataaggca tcgctaattt tctcttcccg atcgttcaac
     4201 cgatccacta aaatttcttt gccctccagc gtttgaaaaa cataccgttg aatgttgcct
     4261 aaaataaatg attccttcca tagaaccgga atcttaaccc cctgaggaac cggttcaaat
     4321 tgaatcagct ccggtcttaa ataataaatg cagcgattgc ccttagcgga aaactcgtgg
     4381 gtatactgat tcagctcata ccattcgaaa cgattatagt tgccgataaa ttccgcaaca
     4441 aatcggctct gaggattctg atacaattcc ttcgaggtgg aaacctgagc cacccgccca
     4501 gcttccatca caaaaatccg atcactgatt cgcatcgcct cttcttggtc atgagtcaca
     4561 aaaatcatcg tcatccctag cgattgctgg atagcgcgca aatcccgctg cagctgtttt
     4621 ctaattcggg catccaaagc acttaatggc tcatccagca gcaggacttt cggttcagtg
     4681 accaatgagc gagccagtgc cacccgttgt cgttgaccgc cagaaagaaa ctggacattt
     4741 ttatctgcct gctccgtcat atccaccaat gatagcatct caaaaacctt ttcctgaatc
     4801 acctcaggac tcagcttctt taccttcaaa ccaaaggcta cattttcaaa aacagtcata
     4861 gttggaaaca atgcatactg ttgaaaaaca aagccgatat tgcgatcctt cgtaggctta
     4921 tcggcaatat tctcattgtc cagcacaatc tcaccgctct ttggttgttc caatcccgtg
     4981 atacagcgca gcaacgtaga ctttccgcac ccgctgggac ctagtaaagt aaccagctca
     5041 cccttttcaa tctgaatttc atcaatagct aaaatctgtt tttcatcaaa aaacagattg
     5101 agatcatgta tttctaaaaa cattttccgt cacaactcct ttaagggctt ctttttcgac
     5161 tgcttctctt ccctcgtctg cttaagggac agctgttgct tttgtcgata agtcagcata
     5221 aaggtagtta aagcaattcc caccataaac agcaaataca aaaccatcac agcgctagcc
     5281 aaatggccgt tttcgttcat ccgccgcagc atatagatgc gtaccgtctg aaatcgaccg
     5341 ccaatcaaca ggttcgtcag catgtactcc ccaaaaaccg aagaaaaaat catcagagaa
     5401 actagggtca caccgatacg gatgtttgga aacagcacct ggataataat cgtagacatc
     5461 ggggcgccca aggtttctgc tgcatccact aattccttca gattaattaa gcgcatctgg
     5521 ttgttaagac taagatagac aatcggcaac gccgtaataa acaatgcacc taccaacact
     5581 acaaacattg ggataccagt ttcagcatac gttcgaagca aagccgttac caaaatcact
     5641 cctggtaaag catacggcag caggagcagc ttttccagcc aacgattcaa acgagggaaa
     5701 tacaaatgaa tccaaataat ggtgggaatc atcaacaaca gaatcgtcac caagaccaca
     5761 cctgctaata aaagtgagcg acccaccgca gcaataaatt ccctgtccgt cagcagctgt
     5821 tggaaccatt gcaaggtaaa atcactaggt aaaagcgatt ttgaccattt agtggagctg
     5881 gcatacaaaa tcgttccaat aatcggcagc aagtaataca ggacaaagca gaacaaaatc
     5941 ggttttatta actttttcac gtctgcacca ccttgccttt cttttgaata atcaacattg
     6001 ccgttaccag caggacacct aaagcgactg ccaacgcact gcctaaatta ggcttggcga
     6061 acacatctcc cgacactaaa gccgcaattc gaatcgttaa aagatttaca ttactgctgg
     6121 tcagggcata cgctgtttca taggtcccca tgccattagc aaataagatg acaaaggtgc
     6181 tggcaatcga tggtcgcaaa aaaggcaaag ccaccttgaa ccaagcatac gctgatgaag
     6241 cgcccagcaa ttgtgcggac tccagccatt cctttttcac ttccttcatg ctgggataca
     6301 aaaacaagac ccctaacgga atttggaaat aggtatacgt gatggtcaat ccccaccaag
     6361 aatacaaatt gaagtccgct agaaatggca aaatcatttt cagcacacct acattcccca
     6421 acaaaataat aaaagagaat gccagcggaa tcccagcaaa attagctgcc aaattggaaa
     6481 caaaggtgat tttctcctgc aatttagtcg atagattcat taagcataaa cataaaataa
     6541 acgtcccgat taacccaatc acactggagc tgacagcaat cagtacgctg ttaacaaagg
     6601 cctgtgtgta atatttgttg ctgaaaatat cactgtaatt ggccaatgtc cactggttag
     6661 ttaaatcact gtgcaagctg cacagaacca taaaaagcag cggcagcagt aaaaatgcac
     6721 agaagataaa ggaggcagga accagaacca agcgtttttt catctctttc atcccctcat
     6781 caattcttga atgtcctgca ttttctctga cggtgcaatt cctaataacc agcaaaacaa
     6841 tggagccgcc agcagctgtg gtacagtttg ttccttcagc ttttggcgtt tatttgaaat
     6901 caaataaaaa ggaacttcac gatgtgcagc caagctgccg ccatgcagtc cgtgcgcatc
     6961 catcccgtga tccgcagtca ccacgacttg ataacctttt tgcagccatt caggaaggta
     7021 aatagccaat aatctgtccg cctgattaac cgctgctgca tattcatgag aatcggcggt
     7081 aaatttgtga ccgacatcat caatattcat cgaatgtacg accaaaaagt ctggctgata
     7141 ctgattcact aaataatggg catccgcaaa taaatgcgaa tcagggtaag tgtcctcact
     7201 ataaaagatg ccatgctgaa tcagtcgctc ctcctgatgc tggatgcgat caaccaaacg
     7261 attatagggg gcttgattgt acaactcgct gtaccagtgg taagcggcgg ctcctgtttt
     7321 tccgccagct tccttaacta attcaaacag cgaccactct ttggatcgct gagcatggta
     7381 attactatag atgccatttt cataggctgg aacgccggtc atcagtactt cgtataacgg
     7441 acgtgaattg ctgggcagct cactttttac cttcaataag cttgcttgct ggtgctcaac
     7501 taaatgattc atgaacccaa gctgggcgac ggctgtgtcg tagcgacagc cgtctagtat
     7561 aactacaatc agtttgtcaa gtctactttg cataggtcag tacctccgcc tgccaaagct
     7621 ctggaagctc tacagaggtc ttgttccaag cttcattgtc ttcaactggc accgccgctt
     7681 cataagcatc ctccggcagt aatttttcag ctacttcttt aggcagctct accttgcgaa
     7741 taggtcgcgc atagcccttt gccaaattga tttgcccttc atcagataaa atatattccc
     7801 gtgctagctt tgccgcattg ggatgagggg catttttatt gattaaggtt gtatacccgc
     7861 taatgatgga gccgtctgtt ggcacagtta cctcaaaacg ctcggagtca atttggtcac
     7921 gataatttag tccattgaag tcccaaacaa ctgccacatc gatttcacct ttttcaagat
     7981 ttgctacgct ggcatcaacg gtggacaaac ggcctttttc tgccattttt ttgtagtaat
     8041 tcagacctgg ctgaatatcc ttttcgtcac ctccgttggc aatcgccgca gccaatacag
     8101 caaattgtgc ttggttggca atcgtcacat cccccacgct aacttgatag tcaccctctt
     8161 gcagctcctt ccagctagtc ggtgcttgct tcacattctc tttatcggtg ataaaggaaa
     8221 tggttcccgt atagcttaac aaccaatccc catcatcatc ctttgcccaa tccggaattt
     8281 cctcccaata gttggtctta taaggtaagg ttaactcttt gctttcagcc aacggaccga
     8341 agctgatacc gacatcacca atatcggcgg ttccattttt tccttctgat tcaaacttag
     8401 ccaattcttc tgcactggac atatccgtat ccacgtgact taagccgtat tctttttcaa
     8461 ggtcgttcca agtgcctacc caatttgccc aagtatccgg cattcccaag ctggcaatct
     8521 ctccttcttc cttggctttt tctataatcg tatctaaaga gtcgtctgcc gcagccgatt
     8581 gcttcgttgc ttcacctgag ccgcagcctg tcagcaggat acttactgtt gctgcacaaa
     8641 ataaaccgat agtcatgtta cgaatttttt cattcatttt cattcattct tctccccttt
     8701 cgataattcc attctaacaa agctttgtaa tgagagaatc agtttcttga aaaggtttca
     8761 tgaacaatgt aaaggttgta tatggtttgt aaatttcgac gaattcccgt taaccttcta
     8821 aagcttttgc ggtatgatag tagaaaaaag gaggctccct atgcagtatt atgatcagca
     8881 tctccacacg tatttttcct ttgattcaga ggaaaaattt gaaaactatt tagcctatca
     8941 gccggagttt ttcgtttcta cggatcactt tgatttaaac aatccatcta ccggaaaaga
     9001 tgacattccg gattatccag cctatataaa caaactggac tctttaaaag aaacctattc
     9061 caccaaattt ctgcgaggta tcgagattgg cgttgttcca ggacaagaag agcggatcgt
     9121 ggattacctc gctcagcatc cttatgattt aaagctggta agtattcatc aaaatggccg
     9181 ctttgattat atggacgatg tggttttaac caaagatccc attcaaacta ccaaggaata
     9241 ttttgatcag atgttgcagg tcttgacgga ttttccagaa ggagatattt tgactcactt
     9301 tgattatgga ttacgccgct tcaccttcac agcggaagaa ttcgcggagc attttgaggc
     9361 tcagctgcta acgattttca aaaaggttgt tgaacgagaa ttggcgatgg agttaaatgc
     9421 caagagcttc ttgaaatacg gcaacgaaga cctttaccgt tatgccgttc ctttgtatca
     9481 aagtgtcggc ggaaagctct ttaccttagg ctcggatgct catgttgccg aagattatca
     9541 gctgggcttt aaaaaaatga gtcagctgtt ggcagactgt caggtagaaa agttgctagt
     9601 cattcaagga aaagaacgcc gcctgacgcc gctgccgatt ttgtgattcc tatataatag
     9661 aagaaagtgg tgaaaaaaat gagcaacctg tgggactaca atcaagaggc acccatccat
     9721 tatttaattg cgcgacattg ggatgcgttg aaaattgagg cggtttgccg atccttgtta
     9781 gcagccgttc caaaacaaca actggaaaat tttttggtgg ctgacagctt gcagcgggaa
     9841 aaagttcagg cttattttgc agcctttaaa gatcagcctt tggaatattt gcatgcgcag
     9901 tttcatcttt tttatcaagt agccgcccca gatgactaca acgatttacg aggacagctg
     9961 caattgacct ttcaagcgga tgaaaccgcc tataccgttt tattgggaat ggctcgcctt
    10021 ggtgatcagg cgaaggtcga gtggcggatt ttcgatattt gatggcagga tcaaggcgtt
    10081 tcagttgcaa accttcctct ctttcaatta gaaaaaccgc agcaggaaga agtttgtgtg
    10141 ctggtcaccg atgctggtga aattcgtctc cgcttatttc ctgaccaagc tcccctggct
    10201 gtagccaact ggcgctccct agctgagcaa ggcgtttatg acaacacacc tttcggctcg
    10261 tgtcattaag gaatttgtta ttcaaggtgg cgctttggat ggttctggtg atgaagctgt
    10321 ttccagctat gagggctttt tcgctgatga agtgcatcgc ggactttatc attttaatgg
    10381 cgccttggcc ttaggggacc acggccccca caccaacggc aaccagtttt ttattgtgca
    10441 aaacacaaag gcgcaggctg acctgctaat gtaaagcaag catacttgga gcttggagga
    10501 ctaccggagc tggatggtcg ttatactgtt tttggacagg tataccaagg gttagaaatc
    10561 gtagcagcga tcgcagctca gccgacaaac agtgaagatg taccgattga gccagtgttc
    10621 attcgacaaa ttaacttcga aaaaacatcg taaaaagaca ctcactgact tcagcaaaag
    10681 ctctattctt gagctggctg cagctagtga gtgtctttca tttatccata agataaagct
    10741 tggattcaat aaataaataa atgttttggt taaagctgtg accagcaaat gaggaaccgt
    10801 gcgagccagt tgatctgcca atgcaaacat ttctttaggt gaaggcgctg gcgtgtctgc
    10861 caacggatac tccagtccca atgcttggta accgtgcata ccatagcagt ggtacggtag
    10921 taattctaac ggtacgaacc gatcaagatc tgccacaaaa caagcaatcg tcagcagctc
    10981 ggtcaggttc atattgatac ctggaaccac aggaactcga attcgcactt cgcagttagg
    11041 atactcaatt aaaaagcgac gaatattctt caaaatttgt tggttatcta agccggtcca
    11101 ttgtttgtgg agcttgctgg taaacatttt tacatccaca aacaatgtat ccacataggg
    11161 agctaccttt tggatttcat tataagcacc acaaaagctg gtttccagta cggtattgat
    11221 tccaatgtat ttcgattttt gcaaaatttc tttagcaaaa tccgcctgaa ccagtgcctc
    11281 gcctccgcta atggtcactc cgccatccga gtgaaagtaa aaaacagcat ccttctcaat
    11341 ctcatccatt acctcttcag cggtcatggt ctggccaaat cctttttcaa tctgcttgct
    11401 ttgtgattcg ggaatcgaac accaggcaca gcgtaatggg cagcccttta aataaacaac
    11461 ggtccgtaaa ccagcaccat catgtagaga accctgttct attcttaata ctgttccttc
    11521 catatagatt tttgaattac acactagttt aaactaaaac acacaaggaa aaacttacca
    11581 tttaatcata aaattagctc atataaatat actaggatgt aaaatagact ggtcaaaatc
    11641 tggtcaaaaa agaggactaa tacaggtagt cctctttttt gttattttaa tattctaaat
    11701 cattttttat tggtttattc ccttattcga tgtagactca aatcactata tcactagata
    11761 tcaaacggcg tcatacggaa tcaaaaacat caaatttgac ctgttaaatg gtcattgaat
    11821 aacattcaaa atcaataaaa cggagtcaga cacggagtca aaaacgctga gagtaaaaaa
    11881 tatatacagc cttgtaggta aagtaccaca gggctgtata ttttttattt cagattacct
    11941 taaatgattc catttacttt ttatctcatt ttttcctact agttagtatc aaatactctc
    12001 ctattttctc aataaactat ttttggatac atggtatctg aaacagctaa aaccttgtca
    12061 ttacagcaaa aaatcacgtt at
//
LOCUS       DAYYNG010000079        12062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000079 DAYYNG010000000
VERSION     DAYYNG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.079"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..511)
                     /gene="frr"
                     /locus_tag="ACHBFA_001754"
     CDS             complement(<1..511)
                     /gene="frr"
                     /locus_tag="ACHBFA_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9395234.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKE"
     gene            complement(514..1236)
                     /gene="pyrH"
                     /locus_tag="ACHBFA_001755"
     CDS             complement(514..1236)
                     /gene="pyrH"
                     /locus_tag="ACHBFA_001755"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9395235.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1372..2253)
                     /gene="tsf"
                     /locus_tag="ACHBFA_001756"
     CDS             complement(1372..2253)
                     /gene="tsf"
                     /locus_tag="ACHBFA_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9395236.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2352..3134)
                     /gene="rpsB"
                     /locus_tag="ACHBFA_001757"
     CDS             complement(2352..3134)
                     /gene="rpsB"
                     /locus_tag="ACHBFA_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9395237.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3493..3972
                     /locus_tag="ACHBFA_001758"
     CDS             3493..3972
                     /locus_tag="ACHBFA_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9395238.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4189..5280
                     /gene="phnW"
                     /locus_tag="ACHBFA_001759"
     CDS             4189..5280
                     /gene="phnW"
                     /locus_tag="ACHBFA_001759"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM9395239.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5273..5401
                     /locus_tag="ACHBFA_001760"
     CDS             5273..5401
                     /locus_tag="ACHBFA_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM9395240.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5405..5950
                     /locus_tag="ACHBFA_001761"
     CDS             5405..5950
                     /locus_tag="ACHBFA_001761"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM9395241.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6404..8095)
                     /gene="argS"
                     /locus_tag="ACHBFA_001762"
     CDS             complement(6404..8095)
                     /gene="argS"
                     /locus_tag="ACHBFA_001762"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9395242.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8515..9462)
                     /gene="arcC"
                     /locus_tag="ACHBFA_001763"
     CDS             complement(8515..9462)
                     /gene="arcC"
                     /locus_tag="ACHBFA_001763"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9395243.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9577..10596)
                     /gene="argF"
                     /locus_tag="ACHBFA_001764"
     CDS             complement(9577..10596)
                     /gene="argF"
                     /locus_tag="ACHBFA_001764"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9395244.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10687..11916)
                     /gene="arcA"
                     /locus_tag="ACHBFA_001765"
     CDS             complement(10687..11916)
                     /gene="arcA"
                     /locus_tag="ACHBFA_001765"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9395245.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3296 a         2508 c         2189 g         4069 t
ORIGIN      
        1 cttctttgat gctgttatct gtcaatgttt gtacgtcttt ttctaatccg cgcaaatcat
       61 cttcggtaat atcaccattt ttttgttgtt ttttatattc atcgatcgca tcacgacgga
      121 tgttacgaat cgcgattttt gcattttctg cttctttttt aacatcttta gctaattctt
      181 ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat gacatttcca tcattggttg
      241 gactgattcc gatatcactt gccataatag ctttttcaac atcttgaaga atgctcttgt
      301 caaaaggtgt gatcattaaa acacgtgctt ctggaatttg gattgatgcc atttggttca
      361 atggtgttgg cgcaccgtag tagttcacag taatacggtc caaaaggcta gcgttcgcac
      421 ggcctgcgcg gatttgccca agttcacgtt gaagattttg tgctgctttt ttcatttttt
      481 cttttgcttc tgtcataatt gcatctgcca tttttatttc ccccttacag ttgttccgat
      541 attttcgcct aagattgcac ggcgaatatt tccgtgttca tttaaattga agacaagcag
      601 tggaatatca ttatccatgc ttagtgaact tgcagttgaa tccatgactt gtaatccttt
      661 ggcaataact tccagatgag tcagttcttc aaattttact gcatttgcat ctactttcgg
      721 atcagcagaa taaacaccgt ctacattgtt tttagccatc aggataacat ctgcgccgat
      781 ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc cggtaccgcc
      841 tgcaaagata acgatccgtc ctttttctaa atgacgttct gctttacggc gaatatatgg
      901 ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac ccacgttttc
      961 caacgtatcc tgtaaagcta aggcattcat aactgtagct aacataccca tgtaatcagc
     1021 ttgagcacgt tccatgccca tttgtgctcc gatttgtccg cgccagatat ttccgccgcc
     1081 cacaacgatt gccatttcaa cgcccagctc gtgaacttct ttgatttctt ggatgatttc
     1141 tttgattact ggtggtttga ttccaaatcc tgcgtcccca gctaaagctt ctccactcaa
     1201 ttttaagact acacgctgat atttcggtgt gaccatgttc attcctccaa tattttctat
     1261 tgccatttta ccatacttag atgatttcga cactctaaga ctggtttttc ttgttttgct
     1321 taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga attatttttt
     1381 cacttggctc ataacttctt ctacaaagtt atcttcgcgt ttttcgatac cttcgccaac
     1441 ttcgaaacgt acgaatgttt taacagttgc gccttttgat gcaacgtatt tttcaacagt
     1501 catatcagga tctttaacga atggttggtc caccaaagcg atttctgctt tgaatttctt
     1561 caagcgtcct tctaccattt tttcaacgat attcgctggt ttgccttcgt tcaatgcttg
     1621 ttctgtaaga acagtttttt catgttctaa ttcagcttct ggaatttgtg attcgtttac
     1681 ataacgagga ttgatagctg ctacgtgcat agctacgtct cttgcaactg tttcgtcagt
     1741 tgttccatct agaacagtca atacggcaat acgtccgccc atatgcaagt atccaccaaa
     1801 tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg aagctgattt tttctccgat
     1861 aacttgagtt gcttcgatca agtcagattc aattgtacct ttatctgttt tgattttcat
     1921 tgcagcttcc atgtctgctg gtttgttttc agcaacaagt tctgcaattt cttttactaa
     1981 atcttggaac atttcgtttt tagaaacaaa gtctgtttct gagtttactt caacgattgc
     2041 tgcagtgtta cctttcacag caactgaagc caatccttca gctgcaatgc ggtcattttt
     2101 cttcgcagcc tttgccattc ctttttcacg taaaagatca actgcttttt ccatgtcgcc
     2161 ttctacttca actaatgctt ttttcgcatc catcattcct acgccagtca tttcgcgtag
     2221 ttcttttacc attttagctg taacgtctgc catgtttaac tcctcctgtg tgtttttctt
     2281 tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg aaacagctcc
     2341 atcattcaaa attattctgc tgaagaattg tcgccttcaa caacatctac gatttcttcg
     2401 attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc ttcaccttgg
     2461 tttccttcga tgaaagcatc agccattttt gaagtaatca atttaacggc acgaattgca
     2521 tcgtcgtttg aagggattac tacgtcgatt tcatctggat cgcagtttgt atcaaccata
     2581 gctacgattg ggatatttaa tttgtgtgct tcttgaacag caatacgttc tttacgtggg
     2641 tcaacgatgt acattacatc tgggattctt ggcatatcag cgataccgcc taagaatttt
     2701 tcaagacgtt cacgttcttt gttcaaacca gcaacttctt ttttaggtag aacttcaaaa
     2761 gttccatctt cttccattgc attgatttgt ttcaaacggc tgatacgttt ttggatagta
     2821 tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg tcctgcacga
     2881 gttgcttctt ctttgattgc ttcttgtgct tgtttttttg tacctacgaa taatgctacg
     2941 ccgccttctt cagcaacgct cttcatgtaa tcataagctg catctactaa tttaactgtt
     3001 ttttgcaagt caatgatgta gattccgttt ctttctgtga agatatattt cttcattttt
     3061 ggattccagc gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa ttgtttcata
     3121 gaaattactg ccatgtttgt ttcctccgat ttggttttat ttttcctctt cttatcttct
     3181 accttgccgg caaacttttt caagcaccta ccgacaatcc aataagaatg tggatttgct
     3241 gatgcataaa gcaccctcag ctattataca gacaaaagat ccgtatcgca agagcaaata
     3301 tacttttctg ctcaacattc aatagtttac ctgaaagata aaaagaattc aagaaaaaca
     3361 gtagatttct ctttgtttct ttattatcct ttttccataa aataatttta ggtataaaag
     3421 acgaatatta ttgggcaatc cttctttttt ttgttatgat acatgtgaaa tctttcgtaa
     3481 aggagaaaga aaatgcgcaa agctcaaaga catttgctga ttaaacaagt cattgaagaa
     3541 ttcccgatcc gtacgcaaga agaactatta aacaaacttg ggcattatgg cgttaccgcc
     3601 acgcaggcaa cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc acctgatgaa
     3661 aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac taggactgag
     3721 gaagaggatc ggctggttca aatgattgaa gacattgtat tgaaagtcga acgagtccac
     3781 tttttgacga tcgtcaatac cttgccagac aatgctcatt tatttgcatc tgttttagat
     3841 gagatcaaac ctccctctat tgtcagtaca attgctggat tcgatacgat catggtcatc
     3901 tcggaaaatg aggaggacgc aaaacgagta gaagaatttc ttcaccatcc aactgaagta
     3961 tcactttttt aatcagagca cgtattccta atatggaatg cgtgtttttt tattttcaat
     4021 attttcctta ttgaataaac ctttacactc tttacaaaaa tacatcattt tttattcaaa
     4081 tttttcaaca ccttgattga atcacaataa tcatcattta tattggatgt taacaagagc
     4141 agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat gtataaatta
     4201 ttaacttctg gaccattgac gacttctgaa acagtgaaaa atgctatgct gatcgatcat
     4261 tgtacttggg atcaagaata caaagaaatt acacaatgga taagaaaaaa attattggat
     4321 cttgctcaag tatcggaagg aatatacaca acggtcttaa tgcaaggtag cagctccttc
     4381 ggggtagaat cggtattaag ttctgtgatc aggccagaag atactgtact gatttgttca
     4441 aacggcgcat atagacaacg aatgatcaac atgtgcgaat gtctgaaact gaactatacc
     4501 gtttatgagg tatcggaaca tcaaatcctc caagctcatg tgattacaca gctgattgaa
     4561 gagaatccgt ctatctccgc actagcaatg gtccatagcg agactacttc cggtattttg
     4621 aataatttgg aagagattgc tgctgtcaca caagaaagag atcttttgtt tatagtggac
     4681 gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg aactagggat tgattttttg
     4741 atttccagct caaacaaatg tattcaaggt gttccaggct tttcttttgt acttgctaat
     4801 aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga tctatatgac
     4861 cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc tacccatacg
     4921 gttttggctt ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat cgaaactcga
     4981 tattcacgtt atctcgccaa taatcatgtg ctacgacaaa aaatgacaga gatggggttc
     5041 cagcctttca ttcaagaata tcaggggcca ttcatcacct cttttcttta ccctaaggac
     5101 tggcgatttg attttaatca tttttatcag tatttaaagc aaaatggatt tgtgatctat
     5161 cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa atattggcga gatctatccg
     5221 gaagatattt tggaactgac agcaattatc aaagactata aggagaacgt taatgattaa
     5281 aactgtaatt tttgattggg caggaacaac tgtggacttt ggctgtatgg ctcctgtcca
     5341 cgcttttaga aatgcttttc tagaaaaagg aacccaactg actgacaaag aaatccgcta
     5401 accgatggga aaattaaaat gggatcatat ccaacagctt ctttccctcc cttctgtcaa
     5461 aaaacaatgg atagctattt atggacaact acctcaggaa aaagaggtgg aggaattgta
     5521 tcaacaattt gaacattatc tgtttaaaga tttggtcaag cattctacct tgaaacccga
     5581 cacattggaa acgatcgaac ggttgagaaa cgctgggatc aatattggtt ccacgaatgg
     5641 atttaatgct cctatgatga caatcgtagc agaaaccgca aaaaaagaaa ggtattcacc
     5701 agacttcatg gcaacttttg aagacgtcga agggtatggt cgaccgtttc cttatatgat
     5761 ccataaaaac atgcagtact tcaaaaacaa gtcggttgat gaagtaatca aagtcggaga
     5821 tacggtctca gatatccagg aagggaaaaa tgccggagtg ttgacagtag gtgtaattga
     5881 aggtagttcg ctaatgggga tttcttatga tgagtaccaa aaattagaga tggggaaaaa
     5941 gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ccggcgctga tatttgtgta
     6001 aacaatttaa aagagttaac agaatacatt ttgtacgctg acaagaatcc agaatgactt
     6061 ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc aagtaataaa aacagccaag
     6121 caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
     6181 gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac gacttttgtc acagtcccct
     6241 tatttgcaaa caaacagtgt ttaagagaca acctctccac taaacggtgt tcattcagct
     6301 gactctcggt atttgctcaa aaaacgataa aatttgaaaa gctattttct cttttgtttc
     6361 tctaatactc ggatcagaac gctgatttca cattctcttt ttcttacatc ttatctggtg
     6421 catgaagtcc tagcaaacga aggtcttctt tcaataatac agtcactgca taaactagag
     6481 ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt cgtatgagca tagtatttgt
     6541 tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac tgacggttcg tatttttccc
     6601 cagcagacaa gaccgtttca gggtattttt gaactaattt aaccacttcc cagctggtat
     6661 cgtcattcaa tgcatagtct gctgtttctg aagggacaaa tcctgctttt tccaacaggc
     6721 tgactgcacg agcatgtgta tattgcacat aaggtccagt ttctccttca aatcgaacaa
     6781 cttcttccag gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg tggaaaatga
     6841 ctgccccaac cccaacttgt ttagctacag catctttgtt ttccaaatca gggtttttct
     6901 cgctgatttg ttctttagca gattggattg cttcatttaa tacttcttct aataagacga
     6961 tctttccttt acgagttgat aattttttac caccttgcgt gatcaaacca aatggaatat
     7021 ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa gacagctttc aactgtttaa
     7081 aatgatagct ttgttcattc cctactacat atagagattg tttgaagtcg taagtacgtt
     7141 tacggtacaa tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg tctgacttct
     7201 taatcaacgc tggattcagg tcataagcag ataggtcaac gatctctgca cctttatcct
     7261 cgttcagcaa atgtttttct tctaaaagtt tgacgatttc atccatcttg tcattgtaga
     7321 aagcttcacc gttcaacgaa tcgaaacgta cttccagcag atcatagatt ttattgaatt
     7381 ctttcatcga ttcatcacgg aaccattgcc ataattgaat agcctcttta tcgccttctt
     7441 ccagacgttt gaaccaagca cgagcttctt catttaattc tggttctgtt tcagctactt
     7501 catggaattg gacgtataaa cgaagcaatt cattgattgg ttctgcttta acagcttctt
     7561 ctgtacccca ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc cagtcaccta
     7621 aatggttgat gcggattggc tgataaccga ttttttccat aatgaaaccg atcgaattac
     7681 cgataactgt cgaacgcaaa tgtcccattg agattggttt ggcaatatta ggagaagaca
     7741 tgtcgattgg tactgttcct tgattcccga tgttgctatc cccataatgt tctttttctt
     7801 tcactactgt ttgcaaaact tttttgctga taagttcttt attcatgaaa aagttcaagt
     7861 aaggtccgac aacttcgatt ttttcaaagt tggcagaatc gattttttct gctaaatctg
     7921 cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct ggaaatgcaa
     7981 catctccgtg ttcagctgat tttggatttt ctagtaattg ctcgacttgt tccagtgtta
     8041 aatcatcttt gacagcgtca tagacagctt ttgcgacaag atctttatta ttcatcttta
     8101 ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa tctctagcga cggcatactt
     8161 agtatgccat cactagagac gagatttccc gcggtaccac tccaattgtc ctaaaaaagg
     8221 accagctcca tctgatatcg gtagacgacc gtctgtttaa aacagaattc tccaagtgcg
     8281 cttcgttgta tcttcttcat tcggcttcca ccacttccga attcgctagc caagaatgat
     8341 acaagtactc tcttgttcaa caaatcttag tcttattaaa ctttcttgaa ttatatcaga
     8401 aaaaaaaacg aaaggctagt cgtttcataa aaaaaaaatc cagatggcct aaaggagaag
     8461 aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa caaattaatc
     8521 agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt ctaaagaagt
     8581 gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgctt ctactttcgg
     8641 aagcatgctt cctggagcga attgcttttc atcaatgtat tgtttcattt ctgaaacagt
     8701 aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat tgtctactcc
     8761 tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg aagcaaaatc
     8821 tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa caggaatccc
     8881 accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga tgacacgtgc
     8941 ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac gtccagcatc
     9001 ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt cttccgtgta
     9061 gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat ccacaataac
     9121 ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta cttcgcccat
     9181 ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg tatctaaagg
     9241 cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt taccgacttg
     9301 cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt tcactaaata
     9361 ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg tagacaagat
     9421 ggcattgccc cctaaagcga caactacttt tcgtttggcc atttcattcg tcctttcttc
     9481 agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt tccctcaatg
     9541 ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga taaacaagtt
     9601 acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg cttggtcaaa
     9661 ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag tgatgccaaa
     9721 gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg gcaagcagtg
     9781 caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat tgatttggta
     9841 aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg atacccatac
     9901 atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg taatcatcaa
     9961 ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt cttcaggttg
    10021 taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg tgactaataa
    10081 agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac cttctactgt
    10141 tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat gccattcatc
    10201 agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta ccattttttg
    10261 actgaatcca cggaattcga ttccgtcaaa catacgtcct agtactttag ctgtatcttc
    10321 tgtggattct tttttaccta attggatatc atttgcacct agatattcag gatgtgcacc
    10381 tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt tttcaaataa
    10441 aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt tcaagtcttt
    10501 caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt ctttttctgc
    10561 taataagctt cttccttgga atactgattc tttcatatta aacattctcc tttagattgt
    10621 ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagact gccgtcagga
    10681 aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg gaccaccacg
    10741 acctcgtgac aattcacttg aattgatttc taacactttg atgccatagc tacgaagcaa
    10801 ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga ttgccaatgt
    10861 gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat taccagttgg
    10921 gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta agttatctga
    10981 atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca tcttaccgtc
    11041 tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg taaatacggt
    11101 atccaaatgc atcattgcac ggttgttcgg aattttgatt gccaacactt tttcaaatcc
    11161 tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat tagttcgttg
    11221 agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc cttcgatatg
    11281 atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc ttggatgatg
    11341 tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag tcatcttatt
    11401 gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca agtttggcat
    11461 tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcatatagac tgctagaacg
    11521 tacttcgata tcttttgtac gaaccccagc catgatttta tctaccattt cttgcgtttc
    11581 cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa tatgagattc
    11641 atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg cttctgccgc
    11701 taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtcttcg caaagttgtc
    11761 atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac gatccataat
    11821 atctggtgtc aaattttcca cttcttgtcc aggccgtttc agtaaaactg tctttaattt
    11881 ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc ttctttttga
    11941 ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct cattcaaaat
    12001 gagacattcc tcacattaat attcgataat taaataatta aacgtttttc tatttttaag
    12061 cg
//
LOCUS       DAYYNG010000080        12036 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000080 DAYYNG010000000
VERSION     DAYYNG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.080"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..296
                     /locus_tag="ACHBFA_001766"
     CDS             <1..296
                     /locus_tag="ACHBFA_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9395246.1"
                     /translation="FIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQRILKR
                     GLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            300..941
                     /locus_tag="ACHBFA_001767"
     CDS             300..941
                     /locus_tag="ACHBFA_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395247.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            938..1855
                     /locus_tag="ACHBFA_001768"
     CDS             938..1855
                     /locus_tag="ACHBFA_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9395248.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            1852..2523
                     /locus_tag="ACHBFA_001769"
     CDS             1852..2523
                     /locus_tag="ACHBFA_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395249.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3064..3552)
                     /locus_tag="ACHBFA_001770"
     CDS             complement(3064..3552)
                     /locus_tag="ACHBFA_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM9395250.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(3753..4019)
                     /locus_tag="ACHBFA_001771"
     CDS             complement(3753..4019)
                     /locus_tag="ACHBFA_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9395251.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4019..4282)
                     /locus_tag="ACHBFA_001772"
     CDS             complement(4019..4282)
                     /locus_tag="ACHBFA_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9395252.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            4803..4943
                     /locus_tag="ACHBFA_001773"
                     /pseudo
     CDS             4803..4943
                     /locus_tag="ACHBFA_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5085..6908)
                     /locus_tag="ACHBFA_001774"
     CDS             complement(5085..6908)
                     /locus_tag="ACHBFA_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9395253.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7506..10211)
                     /locus_tag="ACHBFA_001775"
     CDS             complement(7506..10211)
                     /locus_tag="ACHBFA_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM9395254.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10517..11464)
                     /locus_tag="ACHBFA_001776"
     CDS             complement(10517..11464)
                     /locus_tag="ACHBFA_001776"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM9395255.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11654..11923
                     /gene="rpsN"
                     /locus_tag="ACHBFA_001777"
     CDS             11654..11923
                     /gene="rpsN"
                     /locus_tag="ACHBFA_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9395256.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
BASE COUNT         3428 a         2510 c         2271 g         3827 t
ORIGIN      
        1 gatttatcga tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca
       61 ttttgaataa acatgttttt tacgtaaaag aatccgccta tcttcgtgat accttgcaac
      121 gcattttaaa acgcgggctg aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag
      181 gtatcctgac tcgtgcatca ttagtggata tcgtctacga tgtgatttgg ggagaagatc
      241 ccgcaactac tgatgttgca gagtcagcta ctcctgaaga atctaaggag gtctgaccta
      301 tgcagcagtt ttttcatcag cacggttcag aaattctctc aaaaggtttt gaacatcttt
      361 atatttcagg acttgctttg atcattggta tcatcattgc tgtaccgctt ggtgtcttac
      421 tgacacgttt tccaaaagct gccacgattg tgattggctt aaccagtgcc ttgcagacaa
      481 tcccttctct tgctttattg gcattgatga tcccgctatt tggcgtaggt aaattacctg
      541 cgattatcgc actctttatt tattcattat tgccgatttt acgtaatacg tacattggga
      601 tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg catggggatg actgagttcc
      661 aatccatttt ttccgttgaa ttgcccattg ctatgccaac gatcatggct ggtattcgtt
      721 tagctgccgt ttacgtgatt gcatgggcta cacttgcctc ttatatcggc gctggtggtt
      781 taggcgattt gatttttagt ggactgaata attaccaacc agatctcatt ttggcaggta
      841 cgatccctgt aacaatttta gcactattgg ctgattggct tctaggtctc ttggaacacc
      901 ggctgacacc gatcgcactt agagaggaga atgaagaatg aaacgtatac taaaaatgac
      961 catggttctt ttctctcttc ttatacttgc gagttgttcc ttccctggtc ttgccagcaa
     1021 tacagatgaa gacaccattt cgatcacagg tggaatcact tcagaagcac agatcttagc
     1081 aagtctagtt gccggaatgg ttgaacatta tacagataaa aataccgcaa tcatcaacaa
     1141 tctggctact acaactatca accatcaagc gatgatgaac ggagatgctt caatttcagc
     1201 ggctcgctac acaggaacag atttaacgac tacattgaac cttcctcctg aaaaagatcc
     1261 gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa agatacggtc aaacgtggtt
     1321 cccgtcctat ggttttgaaa atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa
     1381 ataccatcta tctaaagtca gcgatctgaa aaatgtagcg gatgaattag tagctggtgt
     1441 agatacttct tggatcaatc gtaaaggaga cggttatgac ggcttccaag aaacttatgg
     1501 cttctccttc aattcgatat tacctatgca gattggactt gtttatgacg cagtcgaagc
     1561 aggaaaaatg gatattgttc ttggctactc tacggatgga cggatcgcca gttatgatct
     1621 agtcatgttg gaagatgatc gtcaattctt cccaccatat gatgctgctc cagttgtaga
     1681 caacaagata ttagctgata cgcctcattt aaaagaagcc ttaaataagt taggtcatac
     1741 catcagtacg gaaaaaatgc aggaattgaa ctacgaagca gataataatc tagtggaacc
     1801 atcagtagta gcacaacgtt tcttacaaga aaaccactat tttgaaggga agtgaagcaa
     1861 ttggataaaa tgaatgtctt tcaacaattt ttctattatt tccaggaaaa tggctcctat
     1921 atctttgcac aatttatccg tcacttcctg atttctatct atggtgtcct ctttgcagca
     1981 gttgtcggga ttccggttgg gatcatgatt tctaggagac gtaaattagc aaactgggtg
     2041 attcgtttgg cgaatatcat ccagacgatc ccttctctag ccatgatctc cattttaatc
     2101 attggattag gcctgggagt caatgtcgtg attgttactg tatttttgta ttccctctta
     2161 ccgatcatca agaatacgta taccgggatg atccaagtag acaaaaacat ccttgatgtc
     2221 ggcaaaggca tggggatgac tgctagacaa cgactattca tggtagagtt accactctct
     2281 gtttctgtta tcatggcagg cataagaaac gcgttagttg ttgccatcgg gataaccgcg
     2341 atcggtgcat tcgtcggtgc tggtggtctt ggagatatca ttatccgagg aaccaatgca
     2401 acagagggca catcgattat tttagcaggt gcattgccaa ctgcattaat ggcgatcatc
     2461 acggactggc ttttaggtat tttggaaaga cggctagatc cagccagccg tacatcacgc
     2521 tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa aggctttttt
     2581 agttctgtca tttttaaggc aataatccac tctgattctt ccctccttat gggcaaagag
     2641 ttgcttttct tagctattta tccattccgt atactccaac ttcatactgc caacgggttc
     2701 atatctgcat ccaccgacta agtttttttc ttatccgcta aggcattttg ccaatccatt
     2761 ttcccatcac tcgtcatgga attcatacca catgatctct aaatactcgt ctgcttgttc
     2821 tttgaattgg gcatcaattc ttacatcctt tggcgtggct ttcatttaag ccttttccat
     2881 cagtcctcga ttgttcgatg ccttcagctg catcatcaaa actggtgact gccgtatcgg
     2941 taacttcgat catcttttat acaatcggga acttgccatc ctcatccttg gctaaaaaca
     3001 tatctagcca ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac cggtacacga
     3061 ttcttatgga ctgcatccgt cgcaactaga cttggcgata cgatgatcct tcaccagaag
     3121 agcacccccg taaaccaatt gatcgatata ttgccaataa gaaaagacat tggcgtaaaa
     3181 gatatcgatc cctcgtgatg cattgccgtt gatactgcta ttcatggccg aaagcgccac
     3241 gactttaact tcttccgttt gagtgacatt catgttcggc aatgtcgctt tggatacccg
     3301 ctctgctaaa ggaacgacac tggtattgta tttcgtatct gtatcttttc caaaagacca
     3361 tgccaacgaa tcctctggga accaataaga cgactccggt tgattgtcca ttgctttttt
     3421 caacatactt ttggctgttt ctcgataaac gacatttcca attgtcttcg cctcactttg
     3481 gcgataatcg atggtcgttg ccactgccaa cactaacaac gatgctccaa ttttactgat
     3541 tttccttgtc acacttcttc ctccttgttt tacataaacc cgtcaacatt ccattcctaa
     3601 actcaaacaa tgacccttca aaggtggaga tataggaaca aattaaagaa atcatgcaat
     3661 aaatccaaga aggttggttt tagaacaaaa aaagagtctc ctttttgggg agactctttg
     3721 ttaactatct aaaaaggttg gctcttctca cttcactcat aatgtgtcca ggcagaataa
     3781 atttctacaa ctttattttc ctcatcaact ttgtaaacca ctcgatgctg tctattcagc
     3841 ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt caaaagactg cgtgggcata
     3901 tatggatctt ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa attcgtttgc
     3961 ttgatttttc ttaaatccac tttcgcagag tttttgattg cgactgtata gttactcatc
     4021 aaagagcttc ccaatcaata tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt
     4081 ctcgtacttt atctaaagta cctgtttgct caagaaaaag ggtttcttga atcgctctcc
     4141 agtcttccag cccaatcaat acagcattgt ttccactacg atcactaata atctcgattt
     4201 cagtatgatt ttcatttacc tctttcaata actgataaaa atttttacga gcagtgctag
     4261 gattcatcgt tgtattagcc attgtctctc ctcctttttc tgatataatt atagcgtact
     4321 tgattaagta cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact tttcctcttt
     4381 tttacataga gaaaaagacc cctttttagg gaggatcttt gtccacgctc acttcttact
     4441 cctttacgac tagcggtagc caaacgtaaa gtaattggta acaacgtatc atctttagca
     4501 aaggtcgaat ccaaagcctg tgagtctatg caacaacacc aatacacgaa ggttcctcgt
     4561 tgcagtgtga cttgattgat atcttgaata acctgaggat acgcacgaaa gatttgtaac
     4621 ggttattcaa aactgatttt ggccgtcaag tcttctactg cctcaaaaat gattgcctct
     4681 ttcgtctcct gtcattcatg ttcactatcg atttgatact tgcttagacc gttgggtttg
     4741 tgaatcacca attaaaaatg gcactgagtt atggtaacaa aaaatggtca cttgacgagg
     4801 ctgagattcc tacttatgaa gacacatggc acttaagagt cgaggtaaag gaagaagctg
     4861 aaaatatagg aggctgtaca ttatttggca aaggattcgg aaattatgat caagacatca
     4921 aaatcaaaat gttttacagc taaataggaa gaataataac atttcatacg ctgttcccat
     4981 ctttaatggc ttatagaggg cacctttagc tgagaataag acaaaacaga ccctctagct
     5041 tcaattaaga gctagagggt ctgtttttga ttttaaaact tggtttattt cttcaaatgg
     5101 tagctatagg tgctgacaac gatattttca cgccaagaca atcgcaatct taaccgcaaa
     5161 cttgtttggt tatggagaat attctgccag cgtctgtttg gtacaaattg aggaatcaac
     5221 acagtagttg tatagttttg tttcgctgca tttttactta cgatatctac ataacggata
     5281 attggatttt caatcgagcg ataagaagag tggacgattg aaaaacggac atctgggaaa
     5341 tgctttttaa attctgcttc gatttctttt tctttttcga catcttcgtc tagcgaaaca
     5401 tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc
     5461 cgtgtcacat tcccgactaa tacaagaacg gtattcccgt cataatcatg tagttttgca
     5521 tcattttcta aacgaagctg ttctgctact tttttataat gatcatggat cttataaaaa
     5581 atgaacataa cgattggcat aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca
     5641 aacaaaatcg caatgattgc atatgagatg aacgcacccg taatattggc aatcgatttg
     5701 cttaaccatc tttttccttc ttttttccat ttgacgacca tccctgtttg agataaagca
     5761 aatggaatga acacgccgat agagtataac ggaatcaagc gttctgttga gccatggaaa
     5821 ataaacagca atacgatcga acctagcgct agtgtgataa tcccattgga atagcctagt
     5881 cgatccccac gatcttgata catgtgaggc atgaatttat cttttgccag attgtatgca
     5941 agaactggaa aagcagaaaa accggtgtta gcagcaactg ctaaaatcag cgctgtagca
     6001 aattgaagga cgtaatacaa aataccatgt ccaaagacag ctttaccaat ttgagacaaa
     6061 acagtgactt ctttttcagg aacgatccca taccaatagt tgataaatgt gatccccacg
     6121 aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt ttttgctctt
     6181 ggcttcttaa agaatggtac agcattacta atcgcctcca ccccagttaa agaggaagaa
     6241 ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac ccggcacagc cgctcctgga
     6301 agtgcagtag cttgcaatgg cacagcacct gtcacgatat tatataaacc aacaacgatc
     6361 agtaacgaga tcacaaggat aaatgtgtac accggaaata ataggaacga tgcagattcc
     6421 ctcagcccac gaagattcag catcatcagt agcaaaacga ttgtcacaga aatagcgact
     6481 tgatgaccgt agagtgctgg caccgctgat gtgatcgctt cagcacctgc tgagacagat
     6541 actgctactg tcaacatgta gtcgatcaat aaagagccac cggaaatcaa gccagcattc
     6601 tttccaagat tctcactgct gacgacatat gctcccccgc catgaggata agcatggatg
     6661 atttgacgat atgatagcgt taatgaaatc aataaaataa taacaaaagc ggcaatgggt
     6721 agcgaatacc agatggctgc agcagacaaa gcgactaaaa cgacaacgat ttgttccgta
     6781 ccatatgcaa tagatgataa cgcatcagac gacaataatg ccaaagcagc aaatcgggtt
     6841 aatttgtgct catcattttc agcagatttt aaaggcttac ctactaacaa cctttttaag
     6901 taatccaatt tctaaactcc tttacacaca attttacttc aagttttata gtcgaaaatt
     6961 tatagtaaac aagatgttat agtacgctca ctgaccttaa aagtcaataa actttaagaa
     7021 aataattgga aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta atacagcaaa
     7081 aatctaacat ttttttatgg gctttttctt tgaattctat aataaaagga tagaacttga
     7141 cttagaaaat caagttctat cctttttgat atcttaactt ttggaagaga gatgtaaggc
     7201 tgctttctac ccatcattct tactactcga agcatagcgc agttgtcaca acctcttgat
     7261 aaacggtgtt taaaagcttg catctgcggt aataagctca accaaattaa aaacataaga
     7321 agctgttttt atttggtcgt tcttattact tgatgcagag cagctttttt acaacctctt
     7381 ggtacacggt gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg caaaatatga
     7441 ggaactattt ttccattttt ttctctaata ctcaggtcaa aacactgatt tcacaacctc
     7501 tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt cattttttct
     7561 tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacaa accgatcatg
     7621 atccagattg ctttcgtagc atttggccaa taaattcctc ctgcagattc ccgtaacaag
     7681 tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt ccctgagact
     7741 tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat aatagcgatc
     7801 cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat gaacgctaag
     7861 gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac tcctagtaag
     7921 aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc
     7981 atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt tcctaaatag
     8041 tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca tagtgcagta
     8101 taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt ggtttgtagc
     8161 tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa taatttgatg
     8221 acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc tttttggata
     8281 ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt cacagctgtt
     8341 tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc attttttagt
     8401 gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg aatgatcggt
     8461 aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt atccatattt
     8521 ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat ttctttttga
     8581 tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgagct gagaagcgaa
     8641 tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg
     8701 acttggtcta gtagagaagt gacagtggaa ctgatttgac ctgtattgat accagcaatg
     8761 atgccattga tattattaac aacggcatca atttgatcta gcaattcatc aatagccttc
     8821 gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag attgcttagc
     8881 aagggtttta attgatttag ctgatcaata attggctgta atttcgtatt tccagcactt
     8941 gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg ttgagatatt
     9001 ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc agtcagctta
     9061 ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt tgccacttgt
     9121 ccaattgact tcaatgtaac atctacactg tttgtgatgc ttggtatcgc atctttcagc
     9181 tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc taaatttttc
     9241 acatctggca agatatcttg tacttgttgg atgatcgtca agccttgttt tgcttcatcg
     9301 attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc
     9361 ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat ttttgcttga
     9421 tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat ctcatctact
     9481 ttagggatgt aatccacgaa ttcatttgct ttattcagtt tttccttgat ttcaggtagt
     9541 tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa attttcatct
     9601 gttgataaaa tcatatcttt aactttattg atacttacta ggttcgtatc gatgtcgtaa
     9661 cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa ttcatttgtg
     9721 atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat cgcattgatt
     9781 ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt aaaactcagc
     9841 agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc ggaacggact
     9901 ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt atttttatga
     9961 agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc tgctggcttg
    10021 tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc ccataatgct
    10081 ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt
    10141 gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag cgtatttttg
    10201 attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta ccatgtattt
    10261 catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata tcacggcaat
    10321 tgtctcattg taatctctta catattgaga aataattaag gattacagcc tttactttat
    10381 catgaaataa aagggaaaga taattttatg ccaaagtttt tatctattat acgtatacgc
    10441 aaaaaagcta aaacattttt tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt
    10501 tttattcctg tatttcttat ttttcgaatg acaggatgat tttcccagca ggaggatgtc
    10561 cttcaactag ctgatgtgct tggatgacat gcttaagtga agcagacaga ttcattgaaa
    10621 taaaaacatg aaaggctcct ttttggtagg ctttgattat gccagcaaag gcttctgcat
    10681 ctagatattc ctttcttgga acaaacgatt cataaaagcc ttcctttttt tgccgcaaat
    10741 ctagatctgg gaggtcattt aatgcaacat acctgccgcc tggtttcatg atctggatgc
    10801 cggtttctcc tttgatactt cctttcgtcg catcgattac aaggtctgcc tgatcagcaa
    10861 attgcagacc tggatttgtt ttatcataag cagcaaatgc tgaagctcct aattttttta
    10921 ccttttcctc atttttactg gatgcactgg ttaagattcg gattcccttt tcatggagaa
    10981 gctggatcag acttgacccg accgcacctg atgctcctag tatcattaca gtatctgtcg
    11041 gctggatttc cgtcaaatgg ttgattagat tataagccgt aattcctggc gtgaccattc
    11101 cggctgcttc ttcccaactc attttatctg gaatttttgc tacttttgct gatggcaaca
    11161 cgacttcttc tccgtaagtc ccgccgactg catggacagc tactcgatca cctacatgaa
    11221 cagttgtgac atctgaacca atctctgtca caataccagc gccatcatta cctggtacat
    11281 aagggaattt caatgttcgt atctctttca tttctcctaa acgcaaggca acatcataag
    11341 gattcacact gaatgctttt atttctacac gaacatgtcc agaaccgact tctctgggat
    11401 gagcatcgat ttcttcaaaa acatcttgtg cttctccgta accgttaatt gcaaatcttc
    11461 gcatggtcac catctccctt tttctttaag tttacgttta tgtctttatt ttgacaaaaa
    11521 aaatgtcttt tcgttttggc gcatgattca aaacaaaata gttgtctttt ttcgcgtaac
    11581 gtgttaaatt atttatctaa aagcgtaaag attacgattt acgctaataa aaaataattg
    11641 gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac
    11701 gattgaacgc tatgctgctt tacgccagga attaaaagcg caaaaagatt acgaaggctt
    11761 gcgtaaactt cctttagatg ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg
    11821 acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc
    11881 acataaaggt ttgattccag gagtaaaaaa agccagctgg taatccaagt ctggctgtaa
    11941 tgtaaaggag gagggcgcat ggaccactcc ttggatcatc aaaaaagata cgttcgcttc
    12001 ttctagcaga aatgctggta ctagaagcag acgtat
//
LOCUS       DAYYNG010000081        11893 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000081 DAYYNG010000000
VERSION     DAYYNG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.081"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..1093
                     /locus_tag="ACHBFA_001778"
     CDS             149..1093
                     /locus_tag="ACHBFA_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9395257.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            1107..2180
                     /locus_tag="ACHBFA_001779"
     CDS             1107..2180
                     /locus_tag="ACHBFA_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM9395258.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            2170..4341
                     /locus_tag="ACHBFA_001780"
     CDS             2170..4341
                     /locus_tag="ACHBFA_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFM9395259.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(4453..6624)
                     /gene="copB"
                     /locus_tag="ACHBFA_001781"
     CDS             complement(4453..6624)
                     /gene="copB"
                     /locus_tag="ACHBFA_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM9395260.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(6644..8830)
                     /locus_tag="ACHBFA_001782"
     CDS             complement(6644..8830)
                     /locus_tag="ACHBFA_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9395261.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(8830..9039)
                     /gene="copZ"
                     /locus_tag="ACHBFA_001783"
     CDS             complement(8830..9039)
                     /gene="copZ"
                     /locus_tag="ACHBFA_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM9395262.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9052..9492)
                     /locus_tag="ACHBFA_001784"
     CDS             complement(9052..9492)
                     /locus_tag="ACHBFA_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9395263.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9566..10064)
                     /locus_tag="ACHBFA_001785"
                     /pseudo
     CDS             complement(9566..10064)
                     /locus_tag="ACHBFA_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(10349..11815)
                     /locus_tag="ACHBFA_001786"
     CDS             complement(10349..11815)
                     /locus_tag="ACHBFA_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9395264.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         3413 a         2508 c         2120 g         3852 t
ORIGIN      
        1 ttatttttaa ttttactcaa tttaatcgta ttctcatttc aaaacgaatg tactttaaaa
       61 aaacataata aaatcacttt gcttgtagga tgtattccct tccattacaa tgaaataaat
      121 agcaatttta tgagaaggtg atgtgaaaat ggaagaaaaa atcgaagaat ccaaacaaaa
      181 aatcgatcag ttgatacaag aattagaaaa agcggtcatc ggcaaacgtt caactatcca
      241 attgatgatc gtggccctat tagcaggtgg tcatgtctta ttcgaagata ttcccggtgt
      301 aggtaaaacg ttactggtca aagcactagc aaaagctgta cagggaacct tcagcagagt
      361 ccaatgtact cctgatctgc tgcctagtga tattcttggt ttttccgtat acaattcaca
      421 atcaaaagag tttgaatttc gtccgggacc gatctttacc acgatcttgt tggcagatga
      481 gatcaatcgc acgactcctc gtactcagtc agcactttta gaagcaatgg ccgagaatca
      541 tgcaacgatc gacaacaata cataccctct agacaatcat ttttttgtct tagctacgca
      601 aaatccaatc gagtatgaag gaacatatcc tttgccggaa gcacagctcg atcgtttttt
      661 attccgacta caaatcggtt atccttcttt tgatgacgaa cttttgctac ttttagataa
      721 attcgaaaaa gtgttggata acttaaatga agtattgaac agttatgaaa tagaagaaat
      781 aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg gcatcttatg ccttgcagtt
      841 agtgtcagca acaagaagcc atgctgccat ccagcttggc atcagtccac gaggaagcct
      901 ttcctttatt caggcagcaa aagcttatgc tttaatccat gggagaaatt acgtcacgcc
      961 caaagatttg caggatttag ttccttacgt gttttcccat cgcctgattt tttacgatcg
     1021 ttcattgaag gaagacgaaa aaaaacaaat cattcatact cttgttgcac aaattcctgt
     1081 tcctgtgagg tagattgagg tagataatgg aaaaaatcaa agattatttc attaattttg
     1141 tgttatttct ggtatatttc atcattgtgc tttttgctct tgtattcaac aattctttag
     1201 gctggttctt gttggccttt tttactcttt atgtatcatg gagcttgatt tcattaagca
     1261 gctttctaaa gaaactttcg gttacttgta cattgcagcc actcgtttat cgcaaaaaaa
     1321 gtatgcaggt tattttttcc atagcaaaaa agagaacttt ttctttacct ctccctcgtt
     1381 tacaaatcgg cttcccagca atctttgcag aaaaaaaaca agagattttg attcttacaa
     1441 ataaaccgca agaagtccaa ttgctttggc agccaaaaga aagaggatgc catgaaacat
     1501 taactgtcat ttttacagcc tatgatagtc taggactttt cagaaaaagt gcaaaaagag
     1561 agatctcttt tcctgtgact gttctgcctg ctttttgtaa gattcaagca gaaaaattac
     1621 agagagttct ggaaaaaaaa cagcagctga acgcgtacca aagaggtctt gattttcggg
     1681 aacatcgcgc ttatcgtcca ggtgattctt tcaaccgaat cgattggaaa ttgtctgctc
     1741 atactcaaga gtggttatac cgtgaatacg attatcaaga agaagaatgt tctcctcttt
     1801 tttgcttttg gggttcgcac agtccaaaat ttgaacctct gctagacttg tatttttctt
     1861 tatggcattt gagaaaagaa aagcagccgg aacttctgat attaggcaac cgcgcctttc
     1921 atggcaaaga ccccggtcat acctattttg cttctttgga agctggcgat gagaaagctt
     1981 ttttcgcaca cctaaaaaaa tatgcaaaaa aacaaatcgt cctctttgtt cctgagaatt
     2041 ctcctgaagt ctcagaagct attgaaacat tgtcaaaaga tcgaacggtt tatcttgttt
     2101 atttcgtaga aaaacaactc atggtccaag aaaaagacaa gatatgttca ttgccaggag
     2161 gtgaatggat tgacgattaa atgggaaaaa tggctgtgtg gcgtttactc ctttgtgata
     2221 ctaagtgtag gtgctccgat atttgtagta gctaatcaat tgcaaaacag ctaccttctt
     2281 atcctaacta ttggtgtcct atgccttatt gtctgctgta ttcctaaaaa atgggtacgt
     2341 ttgctgcttt ctttgccagt aatgataggt tgtttgtttt tgtattttcc ttcgaagaca
     2401 ttttccttat tatggatctt acagtttttt tcgtcagtca cagaggaatt cttccatcta
     2461 tcccaaggta aactttatta tttgccagaa aagacagctt tttttctcat aatgttgctg
     2521 atctatttat ttattacatt gaccgtggat tacgactatt tgtacgcacc ctttctttct
     2581 ttgatggctt acttcatgat gcttaccgtt tttcgggaaa aagatttact gtacgaaatg
     2641 attgcggtag taactgtttt gttcgtgtat ctggcagcaa ggcaattttt ctccatgcgg
     2701 ttgttgtcgg gaaacactcg attccagtca ttgactacag ttctgcttat ttgtttctta
     2761 actattagcg ctatcttgcc tttatatctt ccgaaagtac accaatctct cgaggaaacg
     2821 agtgaaccga tccgagaata tcttaatgat gaagggcttt atgatacact ccatgagtat
     2881 caattaaccg gaagtgcaag aacaggcttc agtgagaatg atgaacagct tggtggacct
     2941 ttatatgata atggcgaagt tgtttttaca gcgaaccaaa aaggaaatgg ctattggaag
     3001 atcgaaaata aaaatcatta caccggttct ggatggatag aagatcagga cgcacccatc
     3061 caaccaatcc aagaattccc tgttgacatc acgagctacc aaaccgttat ggaagacaaa
     3121 gaaaacatta cattgaccct aaaaaaacaa gctttcttac cttatcctta tggaaatact
     3181 accttgactg atttttcatt agctagtgga caaacgcttt actatttgcc tgaaagccag
     3241 cgattcattc ctgtcccgaa tgaatcgaca cgttctgcaa ctttacaaac agaaaggtta
     3301 gtttttacga gagaagaatt aaaaagcagt cagttaaaag ctgatgattt agaaaatcag
     3361 cagacaaatc ttcaattgcc aaaaagtttg cctaaacgta ttgaggactt ggcggtcgaa
     3421 ttgaccaaaa atgaagcaac tttaattcgt aaagtcgaag ctgtcgaaca gtatttaaaa
     3481 acaagcggaa atttccgcta ttccaaaacg gataccggac atactccgaa agaacacgat
     3541 tatgtggatt atttcctctt tgattcaaaa gtcggctatt gtgacaactt ttcaacttct
     3601 atggtcgtcc ttcttcgaac acttggtatc ccagctcgct ggacaaaagg ttttacaaat
     3661 gggctcagaa caggcacaga taatcaaggg aatgaggaat acagcattct caatagtcat
     3721 gctcattctt ggccagaggt atactttcct ggatttggct ggcttccatt tgaaccaacc
     3781 ccttcatttg ccaatccaat gggttcacaa ccagttgctg aaacaaccga aaccgtccag
     3841 cctgctatcg aacaaccaaa tgaaagcgaa aatatggcag aaactatttc cagtgagaga
     3901 tctgaggtca ttccatcaga agaaacaaca ctcacaccag cttataaaat cgtcttattc
     3961 agccttgggg tgtgtttata cgtcttgttg ttctttctat tgttattcag aaaatctctg
     4021 cgctggacaa gttatcttat ctatcttcat ttttttgctt ctactaatac gattgcatat
     4081 caaaaacttc ttaaccattt ggaaacattg gtaccgcgca aacccgcaga atctttgcag
     4141 gaatatagcc tgcgaataga acaagaattc aagctagatc tgcgtttcat tcgattaact
     4201 actcaatatg aagcagaaat atatggacga caagcagatc cggtaaattt cgataaaaaa
     4261 acctatttag aaattacgca tataataaga aaaacggtat acaaaaaagt tctgacaaaa
     4321 gggagccgct tcaaaaaata agataaaatt tctggagaat ctacttctgg aatgccgaga
     4381 accagctgct tgagtatcga ttattcgaaa aaaaggttgt gacacgtttt ttgtcacaac
     4441 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
     4501 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
     4561 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
     4621 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
     4681 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
     4741 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
     4801 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
     4861 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
     4921 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
     4981 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
     5041 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
     5101 tccgagacgc tcaacttctt tttcattaac tattttcact tgttggtttt tgactgttgc
     5161 ctctagccca actcctgaaa gattttttag attttcggct tgataaggct tgatttcatg
     5221 ttctttcaaa taattcataa taccaacagc caatggatga ttggcattcg cttccaatgc
     5281 tcctaaatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
     5341 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
     5401 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     5461 tgtagagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     5521 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     5581 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     5641 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     5701 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     5761 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     5821 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     5881 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     5941 ttttccatct ttcgtcaagc gttttactga ttctggcagt aattctgcta gtttttgtaa
     6001 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     6061 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     6121 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     6181 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     6241 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     6301 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     6361 gataagcgat agccaaaatt tttgtttgaa atttcccatg tgcatggaat gatccatgcc
     6421 gccatgatcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     6481 atggttatgt cccatgtgca tggaatggtc catctggcta tggtcatgtt tcatctccat
     6541 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gttcatgttt
     6601 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgcc
     6661 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     6721 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     6781 aagcccagaa aagattttgt ttgatttttc tcaatgtcac cctagacaac tcgattgttt
     6841 gtgcaatgga tgccaaactg ctactcatca gcgtcacatc ggctgtttcc atcgcgatat
     6901 ccgttccact tcccattgcc ataccaacat cagctaatgc taaagccggt gcatcattga
     6961 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     7021 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     7081 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     7141 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatca
     7201 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     7261 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     7321 tacccgcaaa atagcgtact ccgttaatcg taccgctgat tccagcacct gggtgagctg
     7381 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     7441 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttctttag
     7501 aaccgactag atctgtaact ttgggctttc cttctgtaat cgtcccagtt ttatccaaaa
     7561 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     7621 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg agtcgctaaa cctaatgcac
     7681 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     7741 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     7801 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     7861 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     7921 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     7981 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     8041 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     8101 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     8161 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     8221 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     8281 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     8341 atacatccat atttggtgct ttcgacttga ttgcatggta cgcaccttta taaaaacgag
     8401 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     8461 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     8521 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     8581 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccatag ccgatgtttt
     8641 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     8701 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     8761 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     8821 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     8881 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     8941 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     9001 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     9061 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     9121 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     9181 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     9241 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     9301 ttgttctgtc cagatcactt ctttttttac caaacgaccc agcaaagttt tgatcgttgc
     9361 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     9421 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctaatcttta ctggcgtttt
     9481 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattaac ttatgaatac
     9541 agattacact tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     9601 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     9661 cttggatcag ctgctgtatt tttggttcat tgtttccttc aaaatgccag ataaaagata
     9721 aaaattctaa gtatttcctg tttagtttct ccgaaaatga ttcaggcata tctggccgag
     9781 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     9841 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aacgtagaag
     9901 tataatttcc ttcaacgatc catgacggat tagataccat aaacgattgc tgttcttgca
     9961 aaaaccactt ttttgcctga atgcggaaag ccggtgattt ctttgatctt ccttgcgagg
    10021 gacgattttc ccgacccact ttgtccaatc acgatgatct tcattcttct ccctcacttc
    10081 tctttggaat ttggttcgat cataccactt ataacgccgc aaaaaaacaa gggactgtga
    10141 caaaagtcgt gtcaaagtcc cttgtttcga ataaatggtg ttcaatcagc tgaccttcag
    10201 cattagctca aaaaacggca aaatatgagg agctattttt gtttggctgt tcttattact
    10261 tgatgcagga cagctttttc acaacctctg attcacggtg ttccagaagt agcttctgtc
    10321 acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct ggttcagtaa
    10381 tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat aagatcaaga
    10441 caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga tcagcagtat
    10501 aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca aagcaccaag
    10561 caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa ttgaatttct
    10621 tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc attggcatga
    10681 caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg ataccaaaga
    10741 tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca ttcaccaatg
    10801 tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc gcatctgcta
    10861 acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt ccaatcatgt
    10921 ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta cctaaaattt
    10981 gaaaagcact gtatgcccca tttgccatca agtctttagg gatattatcg ctagcaaaca
    11041 gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct gctaagaaga
    11101 tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa ggggatattt
    11161 tttccgcccc tccaactgca aatactagca tcgaaacggt tgtgaaataa ctgaaatcaa
    11221 atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc atcgttggat
    11281 tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg aacatcgctg
    11341 ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat aaatataaaa
    11401 aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc gtatttacga
    11461 tatttccatt tccttggact gcccaaccac cagcaatcag aattgcttga ggcttttgtg
    11521 ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc agtcgtttat
    11581 tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg aaggctgatc
    11641 ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc aatagccaag
    11701 aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc atgccccaaa
    11761 ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg ttcattcgtt
    11821 ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt ttaacaaact
    11881 tacaagacag tgt
//
LOCUS       DAYYNG010000082        11835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000082 DAYYNG010000000
VERSION     DAYYNG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.082"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..457)
                     /locus_tag="ACHBFA_001787"
     CDS             complement(38..457)
                     /locus_tag="ACHBFA_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395265.1"
                     /translation="MKKIIVATHYRLAEGFKSTLGYIAPNMVEIIAINAYLENTSVEN
                     QVQAALDQFEAEEQILVFTDLLGGSVNQTFAKFLDRYNIQLIAGVNLPILMTLALAIG
                     SNDLSETTIRQAIEESRQQIVYVNDLLQVSAMDDMDE"
     gene            complement(478..1308)
                     /locus_tag="ACHBFA_001788"
     CDS             complement(478..1308)
                     /locus_tag="ACHBFA_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9395266.1"
                     /translation="MNNERTKGLTQELNKKDRRNAAWRWSFIGSNNINYGTLQGTGYA
                     WSMAKTLRKIYPNDDDYVEAMRVEYEYFNITPYMGPLVAGADVALQEQQGTESLGAVR
                     SIKTSLMGPLSGIGDSLLWVLYPTIMGSISGYMALEGNPLGAIIWIILNLGLVWMRLK
                     LFDLGYESGIRLITDMADKLNAITESASVMGLTVVGSLIATVVKVYTPIEFTFGEVTM
                     GLQAEVFDRILPALLPVLLTALVYFLMEKKKWGFLKVIFLLLAFSLLGAYFGFLGVAP
                     "
     gene            complement(1301..2089)
                     /locus_tag="ACHBFA_001789"
     CDS             complement(1301..2089)
                     /locus_tag="ACHBFA_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9395267.1"
                     /translation="MPEFLQNILIILIPMLTLIDNRGITIINHWPVTVGLLAGLVMGD
                     LQSAMVIAGTFQLMSLGVAALGGSSVPDYGLATVVAIYLNARTGAEIGTSIAVGLPVG
                     ILAINLDIFSRTINSFVSEKSKQYLEERKFSKMQWINFISVFFVAMQAFIPMVILVAF
                     GQVAIETATQWIPEWITNGLNVAGGMLPVVGVAMLMRYMPTKRYIWALLAGFIMAAYM
                     ELPILAVSIVGLALAVFIYNNLTSQKVAVTTENAAYDEGDDFDE"
     gene            complement(2114..2587)
                     /locus_tag="ACHBFA_001790"
     CDS             complement(2114..2587)
                     /locus_tag="ACHBFA_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9395268.1"
                     /translation="MGLVLARIDERLVHGIVVTQWASAVKAKRIMVVDDQVAQDEPRK
                     AAMRLSKPAGTGMSLIDTETGITNFKAGKYDSHNVLLVVNNVKTLLDLSEQGIAIPKV
                     NIGIMLDRNDRKKLAKNFAASPEELQQLQQLADKGIPVSYQFAPSDKEEPLDKFI"
     gene            complement(2604..4661)
                     /locus_tag="ACHBFA_001791"
     CDS             complement(2604..4661)
                     /locus_tag="ACHBFA_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HFM9395269.1"
                     /translation="MLFKSKEVIVSEQSFVVKDRNQCLKVEGWGRDTVRVTCYPNHKG
                     FDPKQGIEELEKNVLTTATVTETSDQLILTNNRLTATYDGEKLVFYNNGTIILEEYSR
                     KQSQVRRTVGIDTHIPIPDEPTSSLNISPREFTYSGENTYQATLRFEGDPNEKLYGMG
                     GYQEENFNKNGGTYELMQRNSQTSIPFYLSDKQYGFIWNNAAIGEVTFGYNQKIWKSN
                     NTNLIDYLVTVGENPKQIIENYTQMTGRTPRLEPELLGLWQSKLRYQTLDELRTVYEG
                     YNQRGISLSVLVVDYYHWTSDGDFAFDSRYWEGIEDFAEEIKKTGTQLMVSLWPTVTE
                     ESRYYQSYRNNQLLIRSVTNKDSLFAGKALLDFFHPESKTFLRQVLDENYRSKGISLF
                     WADQAEPEMDVYQHKEYLIYEGSLEKYANKYPLHYLQAIQKNPERSNTEPTLIRSAWF
                     NAQKYGALAWSGDIESSFDSLRRQVQVGLSMGISGITWWTSDIGGFHSGNSQSPVFKE
                     LMIRWFQFAVFSPILRMHGDRQPHTQKIGNSGGGVRTSGGPNEIWSFGEEVEGILTRF
                     IHIRTRLKDYIRDVYNESATKGLPIMRSLFIEFPDDPKSWEETTHYLFGSDLLIAPVV
                     TEGATHIPVYLPKGCNWIQVFTKKVYPGGQIYKIAVTLNTIPVFCKEGAPLEHHLADI
                     FGN"
     gene            complement(4711..6768)
                     /locus_tag="ACHBFA_001792"
     CDS             complement(4711..6768)
                     /locus_tag="ACHBFA_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HFM9395270.1"
                     /translation="MITIEANRIVKKYDDELLIIEPWGQHSLRVRSFLDQHFVERENA
                     LTELHGYDEKLVETYYKNNQATIVNGKIKAVLDHRDRLTFYNEKDEVLLKEFIRLRAV
                     KHDDGSEDVGTVEFTKDFNSTLKLKSREYRANNDGSNFRTKVRFEPHAQEKIYGMGQY
                     QQNYLDLKNTMLELAQRNSQMSVPFYVSSRNYGFLWNHPGIGKVTFAKNMTEWEMYGT
                     DYIDYWITAGDSPKEITEQYTSVTGRVPEMPKKLLGLWQSKLRYRTPEEVLEVVEGYK
                     QRGIQLSTIAIDYFHWPKQGEYRFDPDFWPEPEKLVKTLKEDYNVEPLISIWPTVQSD
                     AYNYEQYLQGGYLIKVNRGVRLTMQIQGSTVFMDTTNPEARDYVWNLIKKNYKDIGID
                     YYWVDVAEPGYAVYDFENYRYHSGTAVESGNLYPNGYLKMVYDGNKDSNESVVTLVRG
                     AWAGAQKYGALAWSGDIDSSFEAFHNQVNSGLNMGMAGIPWWTTDIGGFHGGNPEDPE
                     FRELMVRWFQYSTFSPILRMHGDRLPHSKPLSNSGGGSMPTGAPNEIWSYGSEVENIM
                     TDYVEIRERMKDYLAESMTEAHELGYPIMRTLFYEFPEDKKSWEIDNTYMLGSDILVA
                     PIMNYKERGREVYLPSGEKWRHLFTGKEYAGGATYYVAAPLEQIPVFIKESASELFES
                     LGK"
     gene            complement(6791..8284)
                     /gene="xylB"
                     /locus_tag="ACHBFA_001793"
     CDS             complement(6791..8284)
                     /gene="xylB"
                     /locus_tag="ACHBFA_001793"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9395271.1"
                     /translation="MGYVLGLDLGTGALKGVLVDETGVIRHIEACDYPLHSPKGGYNE
                     QNPADWLTACGALFEKFSCNVSDFQAELAGISFSGQMHSLVVTDEAGTVLRPAILWND
                     VRNSAQCKRIMDEFGNEIIAITKNRALEGFTLPKILWIQEHEPAIWEKVRKIFLPKDY
                     LRYYLTGHSHMDYSDAAGTLLMDTTKKEWSKPILEKYNIPEVILPPLVESSACVGTLK
                     PEIKATFGFEKEVRIFTGGADNAVSSLGSGLTDSHTALASIGTSGVFLALEEEDHTRY
                     GGKVHLFNHVLPDTYYAMGVTLAAGHSVSWFKNLLAPEQSYDEFLRNIPNVPIGSNGL
                     LFTPYISGERTPHVDSQIRGSFIGLSNHHTRDDLIRAVLEGVTFSLKDSQQLMTSLKG
                     EEFKRIISVGGGAKNKTWLQMQADIFNTEVVSLDTEEGPGLGAAILAAVGLGWFDSFE
                     SCTNQVIRYGTTVQPIPENVEKYNTVYDLYTKVYGATKDLSHALSIL"
     gene            complement(8303..9610)
                     /gene="xylA"
                     /locus_tag="ACHBFA_001794"
     CDS             complement(8303..9610)
                     /gene="xylA"
                     /locus_tag="ACHBFA_001794"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFM9395272.1"
                     /translation="MNYFEAVPKIQYEGKQTKNKFAFRYYNPEEEILGKPMKEHLRFA
                     VAYWHTITADGSDPFGAGTYQRNWEGNNPMETAKNRVEAFFEILEKLGAEYFCFHDVD
                     IAPQGDSLEEFFQNIDTITDLIKEKMDQTGIKLLWNTANMFTHPRFVNGAATTNNADV
                     YAIAAAQVKKGLDISKKLNGQNYVFWGGREGYESLLNTNMDFELDNLARFYKMAIAYT
                     KKIGHQPQFLIEPKPKEPSKHQYDFDAATSMAFIMKYGLQDHFKLNLEANHATLAGHT
                     FEHEINVARSYNALGSLDANQGDLLLGWDTDEFPTDIYSTTLAMYEVLENGGIAPGGI
                     NFDAKVRRTSFEMEDLILAHIVGMDTYARGLRAAAKMKEERFFEEIMEEKYASFTQGI
                     GKKIIDDQETFESLSEYALSLSDVTNQSSHIEYIKSLINDYLV"
     gene            9865..11013
                     /locus_tag="ACHBFA_001795"
     CDS             9865..11013
                     /locus_tag="ACHBFA_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9395273.1"
                     /translation="MNDYTPTHYNESTILEFIIQNAEVSRSKLAKLTNLTKSSISDIT
                     KTLIEKSLVYELNTGMASVSGGRKPIYLSFNNECALVIAIEIGKDYIHSSLSYINGEL
                     VNKLSYHSLLVTKDTLVMLLTKIISDLTSKIPQTPYGILGIGMAIHGIVKDDTVLFTP
                     YSDIDSIPLKDLLAKEIPYPIVLINEANAGALGEYTFKTPSENLVNINIQDGIGAGIV
                     ENGKLFIGANGTAGEIGHTTLYPDGILCPCGNHGCLEHYASTRILLKKLLDSDHKPHP
                     DISVIVKKWHQGDAHTQKILKHNAKLLSIGINNIVLMYDPEVVIINSEIYRGIPELID
                     EIKKHLTGKNTKNLAIKNTSLKGNSTLYGCVSIVAQSFLKINNLKFTV"
     gene            complement(11104..11415)
                     /locus_tag="ACHBFA_001796"
     CDS             complement(11104..11415)
                     /locus_tag="ACHBFA_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395274.1"
                     /translation="MRNKPITVTLFANAYDNELKTNPHSKLKEGTYSVDNDRISFLTK
                     KGEGLVLNLNIQHIDSIATFNQSVIQIKDHNMTEVKNKIYAFSKKSNDKSYWETLAEI
                     I"
BASE COUNT         3237 a         2621 c         2260 g         3717 t
ORIGIN      
        1 agcaaaaaga ttgaacgaat tcaatctttt tgctttttta ttcatccata tcatccatag
       61 ctgatacttg caacaaatcg ttaacataaa caatttgttg tcgggactct tcgatggctt
      121 ggcgaatggt tgtttcactc aaatcgttgc taccgattgc taaggctaaa gtcattaaga
      181 ttggcaaatt aacgcccgca atcaattgga tattgtaacg gtctaaaaat ttggcaaaag
      241 tctggttaac ggaacctccc aacaaatccg tgaaaactaa tatttgttct tcggcttcga
      301 attggtctaa tgccgcttgg acttggtttt caacagaagt gttttctagg tacgcattaa
      361 tagcaatgat ttccaccata tttggtgcaa tatagcctaa cgtactcttg aacccttccg
      421 ctaagcggta gtgtgtagct acaataattt ttttcaccta tttccctcct cgtatgatta
      481 aggtgcgact cctaagaaac cgaagtaggc cccaagtaaa gagaacgcga gcagcaagaa
      541 gataaccttt aaaaagcccc attttttctt ttccatcaag aagtaaacca atgcggtcaa
      601 caagaccggt aacaatgctg gtaagatacg gtcaaagact tccgcctgca agcccatggt
      661 tacttcaccg aaagtaaatt ctattggcgt gtaaacttta acaacagtcg caattaagct
      721 tcccactacc gtcagtccca tgacggaagc tgattctgtg atggcgttga gtttatcggc
      781 catgtcagtg attaaacgta tccccgattc gtaccccaaa tcaaacaatt tcagacgcat
      841 ccagactaat ccgaggttca atataatcca aatgatagcg cctaatgggt taccttccag
      901 agccatgtag ccactgatag atcccatgat agttgggtac aatacccata gtaaggaatc
      961 tccgataccg gacaaaggac ccatcaggga tgttttgatt gaacggacag ctcccaagga
     1021 ttctgtccct tgttgctctt gtagcgctac gtcagcacca gcgactaagg gtcccatata
     1081 aggggtgatg ttgaagtact catattcaac cctcattgct tccacgtaat catcgtcatt
     1141 tggataaatt ttacgcaagg ttttcgccat ggaccaggca tagccggttc cttgcaaggt
     1201 tccgtagtta atgttgttac tgccgataaa agaccagcgc caagctgcat tacgacgatc
     1261 ttttttgttt agttcctgtg taagtccttt tgttctctca ttattcatcg aagtcatctc
     1321 cctcatcgta tgcagcattt tcagttgtta cagccacttt ttggctcgtt aagttattat
     1381 agataaaaac tgccagtgct aatccaacaa ttgaaacggc caagattggt aattccatat
     1441 atgctgccat tataaaaccg gctaacaaag cccaaatgta gcgttttgtt ggcatatagc
     1501 gcattaacat ggctaccccg acgactggca gcataccccc agcgacgttt aaaccatttg
     1561 taatccattc tggaatccat tgggtggctg tttcaattgc tacttgacca aaggctacga
     1621 ggataaccat cggaatgaaa gcttgcatcg cgacgaagaa gacactgatg aagttaatcc
     1681 attgcatctt actaaatttc cgttcctcca aatattgttt agatttttcg gaaacgaaag
     1741 agttgattgt ccgcgaaaag atgtccaagt taatagccaa aataccgact ggtaaaccga
     1801 ccgcgatgga agttcctatt tctgctcctg tacgagcatt caagtaaatc gcaacaacgg
     1861 ttgccaaacc ataatccggt accgatgatc cacccaatgc ggctactcca aggctcatta
     1921 attgaaacgt tccagcgata accatggcgg attgcaaatc tcccatcact aaaccagcta
     1981 aaagaccaac cgttactggc cagtggttaa taatggtaat tccccggtta tcaataagag
     2041 taagcatagg gattaaaata attaagatat tctgtaaaaa ttctggcatt ataattcctc
     2101 caatctcaat ttattaaata aatttgtcta acggttcttc tttgtcggat ggagcaaatt
     2161 ggtatgatac cggaatgccc ttatctgcaa gctgttgcaa ctgttgcaat tcttctgggg
     2221 aagccgcgaa attcttagct aactttttgc ggtcgtttct atccagcata atcccgatgt
     2281 tcactttcgg aatggcgatg ccttgttcac tcaagtcgag taacgtttta acattattaa
     2341 ctaccaacaa cacgttatga ctgtcatatt tcccggcctt aaaattggta atgccagttt
     2401 ctgtgtcaat caaggacatt cctgtccctg ctggtttgct tagacgcata gctgcctttc
     2461 ttggttcatc ttgggcaact tgatcgtcca ctaccatgat acgttttgct tttaccgcac
     2521 tggcccattg tgtcacaaca attccgtgaa ccagtcgttc atcaattcga gctaatacta
     2581 atcccatttt tcatttctcc ttttcaattt ccaaatatat ctgctagatg gtgttcgagt
     2641 ggtgctccct ctttacaaaa taccggaatg gtattcagtg ttacagcaat tttgtatatc
     2701 tgaccaccag ggtaaacttt tttcgtgaaa acttgaatcc aattacaacc ctttggcaag
     2761 tagactggta tgtgtgttgc gccttctgtc acaaccggag caatcaacaa atcgctgccg
     2821 aataaataat gagtggtttc ttcccaagat ttgggatcat ctggaaattc tatgaacaga
     2881 ctacgcataa ttggtaaacc ttttgtagcg ctctcattat atacatccct aatgtaatcc
     2941 ttgagacgcg ttctgatatg gataaagcgc gtcaatatgc cttcaacttc ttccccgaaa
     3001 gaccatattt catttggtcc accgcttgtc cttacccccc ctcccgaatt ccctattttt
     3061 tgggtatgag gttggcgatc accgtgcatc cgtagaatcg gactgaaaac agcgaactga
     3121 aaccagcgaa tcatgagttc tttgaaaact ggggactgac tgtttccact atggaaacct
     3181 ccgatatcgc tcgtccacca agtaatgcca cttatgccca tactaagacc gacttgaact
     3241 tgtcttctta gtgaatcgaa tgaactttcg atatcccccg accaagctaa tgccccgtat
     3301 ttctgggcgt taaaccaggc agaacggatt aaggtaggtt ctgtgttcga tctctctgga
     3361 tttttctgga ttgcctgtag ataatggaga gggtatttat tggcatattt ttctaagcta
     3421 ccctcgtata taagatattc cttgtgttgg tacacatcca tttctggttc cgcttggtca
     3481 gcccaaaaca aggaaattcc cttgctccgg taattttcgt ccaagacctg acggaggaat
     3541 gtcttactct ctggatggaa gaaatctaac agagcttttc cggcaaatag cgaatcttta
     3601 tttgtgaccg acctgatcaa gagttgatta ttccggtagc tctgataata acgactctcc
     3661 tcggttaccg ttggccatag agataccatc agttgggtgc cagtcttctt gatttcttcc
     3721 gcaaagtcct cgattccttc ccaatagcga gaatcaaatg cgaaatcacc atctgaagtc
     3781 caatgatagt aatcaacaac caaaacggaa agagaaatgc ctcgttgatt gtaaccttca
     3841 taaaccgttc ttaattcatc caacgtctga taacgcagct tgctttgcca taaccccagt
     3901 aattccggtt ccaaccgagg cgtgcgccca gtcatttgcg tataattttc tatgatttgt
     3961 ttcggatttt ctccgacggt caccaagtaa tcaataagat tcgtattgtt gctcttccat
     4021 attttctggt tgtagccaaa tgttacttcc ccgattgccg cattattcca gatgaaacca
     4081 tactgcttat cggagagata gaatggtatt gacgtctggg agttccgttg cattaactcg
     4141 tacgttcccc cgtttttgtt aaagttttct tcttgatatc cgcccatccc gtacaatttt
     4201 tcattcggat caccttcaaa acgaagagtt gcttggtagg tgttctcgcc agaataggtg
     4261 aattctcgag gtgaaatatt taacgatgag gtcggttcgt ccggaatcgg aatgtgggtg
     4321 tcaatgccta cagtacgtcg gacttgactt tgcttccgag agtattcctc caggattatt
     4381 gtcccgttgt tgtaaaagac caacttttct ccatcgtatg tggctgttaa acggttgttg
     4441 gtcaagatta attggtccga cgtctctgta acggttgctg tagtcaagac gttcttctcc
     4501 aattcttcta tcccttgttt tgggtcgaac cctttgtggt tgggataaca ggtaacgcgc
     4561 accgtgtcgc gtccccatcc ttccactttc aggcattgat tacggtcctt cacgacgaag
     4621 gactgttcgg aaacaatcac ttcttttgat ttaaagagca tagcttcttt cctcccaatt
     4681 gacttgtcct ttatctcctc tctttatggg ctatttgcct aacgattcaa aaagttcaga
     4741 ggcggattct ttaataaata ccgggatttg ttcaagaggt gctgcgacat aataagtcgc
     4801 tcccccagca tattctttac cggtgaataa gtgtcgccat ttttcaccgc ttggtaagta
     4861 aacctcccta cctctttctt tgtagttcat aatcggagca accaggatgt cagaaccaag
     4921 catgtaggta ttatcgattt cccaggattt tttgtcctct gggaattcgt agaataatgt
     4981 ccgcatgatt gggtatccga gttcatgtgc ttctgtcatt gattctgcca agtagtcttt
     5041 catccgttct ctaatttcca cgtagtccgt catgatgttt tcaacttcac tgccgtaaga
     5101 ccaaatttcg tttggtgcac ctgttggcat cgaaccacca ccagaattgg agagcggttt
     5161 actatgtggc agacggtctc catgcatgcg caaaatagga gaaaacgtgc tgtactggaa
     5221 ccagcgaacc atcaattcgc ggaattccgg atcttccgga ttaccaccat ggaagccccc
     5281 gatgtcagtc gtccaccatg gaataccggc catccccatg ttcaatccgg agttgacttg
     5341 attatgaaag gcttcgaaac tggaatcaat atccccagac caagccaaag caccatactt
     5401 ctgagcacct gcccaagcac ctcgtaccaa tgtgacaacg gattcatttg aatccttatt
     5461 gccatcgtaa accatcttta agtatccgtt tggatacaag ttgccacttt ccaccgccgt
     5521 tccggaatgg tagcgataat tttcgaaatc ataaacagca taacctggtt cagccacatc
     5581 cacccagtag taatcaatac cgatgtcttt ataatttttc ttgattaggt tccagacata
     5641 atcacgggct tcaggattag tcgtatccat gaaaacagtg cttccttgga tttgcatcgt
     5701 caagcgaact ccgcgattca ctttaatcaa atagccacct tggagatatt gctcatagtt
     5761 ataggcatct gattggacag ttggccaaat cgaaatcaat ggttcgacat tgtaatcttc
     5821 cttgagtgtt ttgaccagtt tttctggttc tggccaaaaa tcgggatcaa atcgatactc
     5881 cccttgcttc ggccagtgga aatagtcaat agcaatagtc gatagctgaa tgcctcgctg
     5941 tttgtatcct tctaccactt ctaagacttc ttcaggagtg cgatagcgta gtttactttg
     6001 ccataatccc agcagttttt tcggcatttc aggaacacgt cccgtaacgc tcgtgtattg
     6061 ctccgtaatt tctttaggtg aatctccggc tgtaatccaa taatcgatat aatccgttcc
     6121 gtacatttcc cattcggtca tgttctttgc aaaggttact tttcctatac caggatgatt
     6181 ccacaagaaa ccgtagttcc ggcttgaaac gtaaaaggga accgacattt gtgaattacg
     6241 ttgagccaat tcgagcattg tgttctttaa atcaagataa ttttgttggt attgccccat
     6301 cccatatatt ttttcctggg catgcggttc aaatcggact tttgttcgaa agttcgaccc
     6361 gtcgttattt gcacggtatt cacgagactt caactttaaa gtggaattaa aatctttggt
     6421 aaattcgact gtcccaacat cctcactgcc atcgtcatgc ttaacggccc tcagacgaat
     6481 gaattctttt aagagaactt catccttttc attgtaaaac gtcaaacgat ctcgatgatc
     6541 aaggacagct tttattttac cgtttacaat cgtagcttga ttgtttttgt agtatgtctc
     6601 aaccagcttt tcatcatacc catgcagttc agttaatgca ttttcccgct caacaaagtg
     6661 ttgatccaaa aacgaccgga ctcgaagtga atgctggccc catggttcaa taattagtaa
     6721 ttcatcatcg tattttttta cgattcgatt tgcttcaatt gtaatcatga tttcctccta
     6781 ttagaattac ttacaatata ctaagcgcat gagacaaatc ttttgtcgca ccgtacacct
     6841 tcgtatataa gtcataaaca gtattatatt tttctacgtt ttccggaatt ggttggacag
     6901 tagtaccata ccgaatgacc tggttggtac aagattcgaa agaatcgaac caacctagac
     6961 cgactgcagc caagattgct gctcccaaac ccggaccttc ttccgtatcc aggctcacga
     7021 cttccgtgtt aaaaatatca gcctgcatct gtaaccaagt cttgttttta gcgccgccac
     7081 ccacactaat gatgcgcttg aactcttcgc cttttaaaga agtcataagt tgttgagaat
     7141 ccttcaagga aaatgttacc ccttccaaaa cagcccgaat caggtcatcc cgggtatggt
     7201 gattactcaa tccgatgaag gacccacgga tttgactgtc aacgtgtggt gtgcgttccc
     7261 cagaaatata cggggtaaat agtagtccat tactgcctat cggcacatta ggaatgtttc
     7321 ttaagaattc atcgtatgat tgttccggtg ccagtaaatt tttgaaccat gagacgctat
     7381 gtccagcagc caatgtgacg cccatcgcgt agtaggtatc tggcaataca tggttgaata
     7441 gatggacttt tccaccataa cgcgtgtgat cctcttcttc cagggccaag aacacaccgg
     7501 aagtaccgat acttgctaaa gcggtatggg aatccgtcaa tccagatccc aatgaggaca
     7561 cagcattgtc ggctccaccg gtaaaaattc gtacctcttt ttcaaagcca aaggtcgctt
     7621 tgatttctgg tttcaatgtt ccaacacaag cggatgattc cactaatggg ggtaagataa
     7681 cttcgggaat gttatatttt tccagtatag gtttggacca ctctttctta gtcgtatcca
     7741 tcaataaagt accggcggca tcggaataat ccatgtggct gtggcccgtc aggtaatagc
     7801 gtaagtaatc cttgggtaaa aatattttac gtaccttttc ccagatagcg ggctcatgtt
     7861 cctgtatcca aaggattttt ggtaaggtga atccttccag agccctgttc ttagtgattg
     7921 caatgatttc attcccaaat tcatccataa tgcgcttaca ttgggccgaa tttcgaacat
     7981 cattccatag gatagccggt cgtaagacag ttcccgcttc atctgtaacg accaaggaat
     8041 gcatttgtcc ggaaaaacta attcctgcta gttcagcctg gaaatcagag acattgcaag
     8101 aaaatttttc gaacaatgca ccacaggctg tgagccaatc agcgggattt tgttcattat
     8161 agcccccttt tgggctatga agggggtaat cacaagcttc aatgtgccga attaccccgg
     8221 tttcatccac caacactcct ttaagggccc ctgttcctaa gtctagccct aatacataac
     8281 ccatgtttta caccccgact ttctatacta aataatcatt aatcagtgat ttgatgtatt
     8341 cgatatgact tgattggttt gtcacatctg aaagggaaag tgcgtactca ctaagcgatt
     8401 caaaggtttc ttggtcatca ataatttttt taccgattcc ttgggtgaag cttgcatatt
     8461 tttcttccat aatttcttcg aagaaacgtt cttctttcat cttagcagcg gcacgcaaac
     8521 cacgtgcata agtatccatc cctacgatat gtgctaaaat caaatcttcc atttcgaagg
     8581 aagtccgacg tactttagca tcgaagttga taccacctgg tgcaatcccc ccattttcca
     8641 agacctcata cattgccaaa gtcgtggaat aaatatctgt agggaattcg tctgtatccc
     8701 accccaacaa caaatcacct tggttagcat ccaacgaacc caatgcgttg taactgcggg
     8761 ctacattgat ttcgtgttcg aacgtatgtc ctgccaacgt agcatggttg gcttcaaggt
     8821 taagtttaaa gtggtcttgt aaaccatatt tcatgatgaa cgccattgaa gtagctgcat
     8881 caaaatcata ttggtgtttt gatggttctt ttggttttgg ttcaatcaga aattggggtt
     8941 gatggccaat ttttttcgta taggcaatcg ccattttgta gaaacgagcc aagttatcca
     9001 attcaaaatc catgttggta ttcaataaac tttcatagcc ttctcttcca ccccaaaaaa
     9061 cataattttg accatttaat tttttgctaa tatctaaacc tttttttact tgtgctgcag
     9121 caatagcgta gacatcagca ttattagttg ttgctgctcc gttgacaaaa cgaggatgtg
     9181 taaacatatt agcagtgttc caaagtaatt taatgcccgt ttgatccatt ttttctttaa
     9241 tcagatcggt aatggtatcg atgttttgga aaaactcttc caaggaatca ccttgaggag
     9301 caatatcgac atcgtggaaa cagaaatatt ccgctcccaa tttttctaaa atttcaaaga
     9361 aagcctccac acggttttta gccgtttcca ttgggttgtt gccttcccaa tttctttggt
     9421 aagtacctgc accgaatgga tcggagccgt ctgccgtaat tgtgtgccag tatgctaccg
     9481 caaaacggag atgctctttc atcggcttgc ctagtatttc ttcttcggga ttatagtagc
     9541 gaaaagcaaa tttattttta gtctgtttgc cctcgtattg tatttttggt accgcttcaa
     9601 aataattcat tgctaattcc acctttctta gttagttcga aactcgtact aacctgatat
     9661 gattatatta cctgggtggg aacagcttgt caagcgcttt cgttttttaa aaattacctt
     9721 ggttggttag gtgcgattta tattttttcc ctgggacttc ctttagaatc cttgacaaga
     9781 ttgaaattat agtaaatgat ttaataaaat ctatttcata tttgaagtat ttcgaaatta
     9841 tcttaattaa aggagcagac taaattgaat gattacacac cgacacatta caatgagtcg
     9901 accatcctcg aattcatcat tcaaaacgcc gaggtttctc gatcaaaatt agcgaaatta
     9961 acaaacttaa cgaagtcgtc tatttccgat atcacaaaaa cattgattga aaaatcactc
    10021 gtctacgaat taaacactgg aatggcctcc gtatcaggcg gtagaaaacc tatttacttg
    10081 agttttaaca acgaatgcgc ccttgttatt gcgatagaga ttgggaagga ctatatccat
    10141 agttctttgt cctacatcaa tggggagttg gtcaataaat tgagttacca ttccctctta
    10201 gtaaccaagg atacacttgt catgttgttg acaaaaataa tctccgattt aacttcgaaa
    10261 ataccccaga ccccttatgg tattttagga attgggatgg caatccatgg catcgtgaaa
    10321 gatgatacag tcttgtttac gccttattca gacattgatt ccattccttt gaaagaccta
    10381 cttgccaaag aaattcccta cccaatcgtc ttaattaatg aggcaaacgc gggtgctctt
    10441 ggggagtata cgtttaaaac cccttcggag aatttggtta acatcaatat tcaagacggg
    10501 attggggctg gtatagttga gaatggtaag ctgtttatcg gggcaaacgg cacagcaggg
    10561 gaaattggac acactaccct ttaccccgat ggcattctat gtccgtgcgg gaatcatggg
    10621 tgtttggagc attatgcttc tacccgtatt ctgttgaaga agctgcttga ttcagatcat
    10681 aaaccacatc cggacatctc agtaatcgtt aaaaaatggc accaggggga tgcacatacc
    10741 cagaaaatac tgaagcataa tgcgaaatta ctcagcatcg gtattaacaa tattgtgcta
    10801 atgtatgacc cagaggttgt catcatcaac agtgaaattt accgaggcat tcccgaattg
    10861 attgacgaga ttaagaagca cttgactggt aaaaacacaa aaaacttagc tattaagaat
    10921 acatctctaa aaggtaattc tacactctat ggctgtgttt ccatagttgc ccagtccttt
    10981 ctgaaaatca ataatctgaa atttacagtg taaagtgata gaaaaatatc ctccttaaaa
    11041 cttcctattt ttcgggagga tatgatttta ttagggtgta agtacttagc agcatttttt
    11101 tcactaaatg atttcagcta acgtttccca ataactttta tcatttgatt ttttagaaaa
    11161 agcataaatt ttgtttttta cttccgtcat attatgatct tttatttgaa taacgctctg
    11221 attgaaagta gctatactgt caatatgctg aatatttaaa tttaacacta atccttcccc
    11281 tttttttgtt aaaaatgaaa tccgatcatt atctacgcta taagttcctt cttttaattt
    11341 tgaatgagga tttgttttta gctcgttatc ataagcgtta gcaaataacg taacggttat
    11401 aggtttattt ctcatttttt cgtctctcct ttttggtcta gagttttaca acttttttct
    11461 tggcgtttgt caagaagcct atcttttgat cttatttttt atttttttaa ttatcttctg
    11521 ttctgttcat acagttgact cctttcttat ttagtccttt tttcaagtat gtccgtctgt
    11581 cgcctgtttt gcccttatca tgagggattt agggaagtaa agggcgagtg tagcgagtgc
    11641 attttaccct ttacttccct aaagaactta tgatataaac cggttggcga gagactgact
    11701 tgaaaatagg acgtgaatag aagtaatagg ttatgaacaa acgggtaatt ttttcaataa
    11761 aaaaacgaac aaaagcagca atctttttcg attgctgctt ttgttcgttt catctgtttt
    11821 aattgcattt atttt
//
LOCUS       DAYYNG010000083        11729 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000083 DAYYNG010000000
VERSION     DAYYNG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.083"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1128
                     /locus_tag="ACHBFA_001797"
     CDS             112..1128
                     /locus_tag="ACHBFA_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9395275.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1269..2570
                     /locus_tag="ACHBFA_001798"
     CDS             1269..2570
                     /locus_tag="ACHBFA_001798"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9395276.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2616..3278
                     /gene="deoC"
                     /locus_tag="ACHBFA_001799"
     CDS             2616..3278
                     /gene="deoC"
                     /locus_tag="ACHBFA_001799"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9395277.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3299..3697
                     /locus_tag="ACHBFA_001800"
     CDS             3299..3697
                     /locus_tag="ACHBFA_001800"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9395278.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3763..4845
                     /locus_tag="ACHBFA_001801"
     CDS             3763..4845
                     /locus_tag="ACHBFA_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9395279.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5057..6619
                     /locus_tag="ACHBFA_001802"
     CDS             5057..6619
                     /locus_tag="ACHBFA_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395280.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6612..7745
                     /locus_tag="ACHBFA_001803"
     CDS             6612..7745
                     /locus_tag="ACHBFA_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395281.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7745..8701
                     /locus_tag="ACHBFA_001804"
     CDS             7745..8701
                     /locus_tag="ACHBFA_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395282.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8800..9966
                     /gene="deoB"
                     /locus_tag="ACHBFA_001805"
     CDS             8800..9966
                     /gene="deoB"
                     /locus_tag="ACHBFA_001805"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9395283.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            9990..10808
                     /locus_tag="ACHBFA_001806"
     CDS             9990..10808
                     /locus_tag="ACHBFA_001806"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9395284.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10825..11532
                     /gene="deoD"
                     /locus_tag="ACHBFA_001807"
     CDS             10825..11532
                     /gene="deoD"
                     /locus_tag="ACHBFA_001807"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9395285.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         3701 a         2101 c         2730 g         3197 t
ORIGIN      
        1 cggtagataa aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct
       61 tgggggtttt ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg
      121 accatcagag atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg
      181 aataacaaag gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat
      241 gaacatcatt ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc
      301 atcggaatga tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta
      361 gagagctatt tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa
      421 gaaaatgaga tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg
      481 gccacaccaa atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg
      541 ccaatcattt taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag
      601 gaatatgagg gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc
      661 ggcatgctga aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt
      721 catgcgctga aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg
      781 actgtccaag gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct
      841 attttttgcg ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc
      901 aaaaaaatcc cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac
      961 atgattccgc aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca
     1021 cgtttcctga tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa
     1081 acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata
     1141 ttgcttatgt aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt
     1201 ttactaaagt ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg
     1261 atcaggcaat gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga
     1321 aagaggaaat cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag
     1381 tcagtgcatt attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact
     1441 taacattggc gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa
     1501 aggtagataa gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc
     1561 tagtagctag tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag
     1621 gtggtacact agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag
     1681 aatttgtacg cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg
     1741 caccggcgga caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc
     1801 cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt
     1861 tggatgtgac cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg
     1921 caaagaccat gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag
     1981 atatgtcaga acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg
     2041 aaacattaca aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc
     2101 aaatggttgt tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag
     2161 aagctctgga atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg
     2221 gagacccagc gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc
     2281 ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg
     2341 caatgatgct aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat
     2401 tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct
     2461 atagtaacga cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg
     2521 gtgaatcggc aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag
     2581 cggactttac cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc
     2641 atactatttt gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa
     2701 aaaaatacca tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac
     2761 aattacaagg tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca
     2821 cacccgagac aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg
     2881 atatggtcat caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata
     2941 tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat
     3001 cgttattaga tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg
     3061 attttgtcaa aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt
     3121 tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg
     3181 aagaagaagc aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag
     3241 tagcaatcgt atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat
     3301 ggcagaactc aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt
     3361 tccttattcc cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca
     3421 aggcatcaac attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt
     3481 tttcaaagca gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac
     3541 ccccgatcct atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc
     3601 tgatatgccg gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc
     3661 actgctgcct tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga
     3721 ctgttgaaaa ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta
     3781 tttggtttag gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac
     3841 ggctctactg attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc
     3901 acagacacag gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa
     3961 aaatggggga aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct
     4021 tcaaatgaat cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg
     4081 atttttggta ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca
     4141 ggtacgaact ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca
     4201 actttcaaag acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa
     4261 acaaacgtag tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct
     4321 ggattcaaag ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg
     4381 aatcaatatg ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg
     4441 tatgcacaaa acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc
     4501 caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac
     4561 cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg
     4621 acatctacac tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac
     4681 ggtaaattcc ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta
     4741 acagaaggac aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc
     4801 atttcaggtg acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc
     4861 cttgttagac acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat
     4921 ccaagctgcc ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca
     4981 aaagaagaac gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt
     5041 aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa
     5101 agaatttgga acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat
     5161 tcatgcgttg cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg
     5221 attattagag ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag
     5281 tccgaccaaa gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga
     5341 tgcctttact gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct
     5401 tgaccataaa aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt
     5461 caacccagat gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt
     5521 aaaaacactt taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac
     5581 ccctcaagaa attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc
     5641 gatcattttg attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt
     5701 tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt
     5761 agccgatatg atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc
     5821 tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc
     5881 cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga
     5941 tggaaacggg cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg
     6001 acatatcaag ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca
     6061 tggtgtagga cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt
     6121 atctgaaaat atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct
     6181 gaattattca gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac
     6241 aacgaatgaa cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat
     6301 cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact
     6361 ggatgtaggt gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa
     6421 agcagtgtta ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc
     6481 tgttatccat gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga
     6541 attaggtcta ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg
     6601 ggctggcgca catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt
     6661 cagttgtttt aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca
     6721 tccaaggtta ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa
     6781 cgttaaggca agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag
     6841 ctgggttttt caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct
     6901 gggtggcact tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct
     6961 taggtgcctt agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg
     7021 gaacaagtga agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat
     7081 ttgctttaca tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga
     7141 tcagtcaaaa tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg
     7201 taaactcagg attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga
     7261 aaacgacttt aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg
     7321 caggaatgag tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag
     7381 ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt
     7441 cgctatctat tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg
     7501 gtattctttt atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga
     7561 caggagcagg tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg
     7621 tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag
     7681 aagagatcgt tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac
     7741 tataatgtcg actatcgaat taattgcctt gatccttact tctacactgg tttattcaac
     7801 accgctaatt ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt
     7861 agggctagag ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat
     7921 gagtccatca cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg
     7981 tatcctattt tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat
     8041 cagcgggaca gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg
     8101 gtatggtaaa ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg
     8161 attagcagat attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta
     8221 ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt
     8281 gcgcctccgt tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata
     8341 tgctatgcgt tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt
     8401 ttttgctcag tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat
     8461 ctccatggct gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt
     8521 gttctttggt tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc
     8581 gattccgcca gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt
     8641 tttcttaggg aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata
     8701 atcttttagc gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt
     8761 tttaaaggca ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg
     8821 atcgtcatgg attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt
     8881 ggttctgata cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag
     8941 cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa
     9001 ggctatgcga cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg
     9061 gaaatcatgg gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat
     9121 gatctgatca aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat
     9181 agcggaacag ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt
     9241 gtatatactt ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta
     9301 gaagaattat atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg
     9361 attggccgaa tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc
     9421 aatcgccatg attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa
     9481 gcaggaaagg acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc
     9541 actgaatctg tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg
     9601 aaaaacgatt tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt
     9661 catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca
     9721 gaattgcttg aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac
     9781 gatccgacgt ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt
     9841 aaaaaaatga aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg
     9901 attgctgaga actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta
     9961 aaataatctt tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa
    10021 cgacccagtt tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt
    10081 caggattagg tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga
    10141 tcccgttctt tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc
    10201 tggcaggcaa gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca
    10261 tgcaaacagt gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg
    10321 tgactaatgc atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta
    10381 ccgatcatat caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg
    10441 gccctagatt tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag
    10501 cagcagcaga aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac
    10561 caacttatga aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag
    10621 ggatgtcgac tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa
    10681 tctcttgtat taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg
    10741 tagaaacaac tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat
    10801 tattgtaata ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc
    10861 gccgataaga ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt
    10921 ttggaagatc cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat
    10981 aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat
    11041 gcccatgaat tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt
    11101 gcattgtcaa aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt
    11161 tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat
    11221 ctattactga aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat
    11281 gttctttcgg aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa
    11341 cttggcgttt taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac
    11401 catgtgcaga cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca
    11461 acagctgcag aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg
    11521 atagcagaat aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag
    11581 caaaaatagc tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt
    11641 gaacaccgtt taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat
    11701 ttcaaaaaac agcttttcat gtttgatga
//
LOCUS       DAYYNG010000084        11679 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000084 DAYYNG010000000
VERSION     DAYYNG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.084"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1373
                     /locus_tag="ACHBFA_001808"
     CDS             72..1373
                     /locus_tag="ACHBFA_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9395286.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1442..2041)
                     /locus_tag="ACHBFA_001809"
     CDS             complement(1442..2041)
                     /locus_tag="ACHBFA_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9395287.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            2295..3176
                     /locus_tag="ACHBFA_001810"
     CDS             2295..3176
                     /locus_tag="ACHBFA_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9395288.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            3189..4799
                     /locus_tag="ACHBFA_001811"
     CDS             3189..4799
                     /locus_tag="ACHBFA_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395289.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFRLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(4876..5543)
                     /locus_tag="ACHBFA_001812"
                     /pseudo
     CDS             complement(4876..5543)
                     /locus_tag="ACHBFA_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
     gene            complement(5826..6491)
                     /locus_tag="ACHBFA_001813"
     CDS             complement(5826..6491)
                     /locus_tag="ACHBFA_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM9395290.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(6638..7243)
                     /gene="dhaL"
                     /locus_tag="ACHBFA_001814"
     CDS             complement(6638..7243)
                     /gene="dhaL"
                     /locus_tag="ACHBFA_001814"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM9395291.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(7271..8281)
                     /gene="dhaK"
                     /locus_tag="ACHBFA_001815"
     CDS             complement(7271..8281)
                     /gene="dhaK"
                     /locus_tag="ACHBFA_001815"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM9395292.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(8256..8651)
                     /gene="dhaM"
                     /locus_tag="ACHBFA_001816"
     CDS             complement(8256..8651)
                     /gene="dhaM"
                     /locus_tag="ACHBFA_001816"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9395293.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8805..9125
                     /locus_tag="ACHBFA_001817"
     CDS             8805..9125
                     /locus_tag="ACHBFA_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM9395294.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(9237..10094)
                     /locus_tag="ACHBFA_001818"
     CDS             complement(9237..10094)
                     /locus_tag="ACHBFA_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9395295.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(10202..11506)
                     /locus_tag="ACHBFA_001819"
     CDS             complement(10202..11506)
                     /locus_tag="ACHBFA_001819"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9395296.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         3264 a         2402 c         2155 g         3858 t
ORIGIN      
        1 cctcttattc tttcttcgtt acaataagga atgggacaag taaaaaaatg aaaaagcaag
       61 ggacgaggta tatggaaaaa aagcaaaaaa ttattttagg agccacttct gctttaacta
      121 tcgttatttt aattctaagt ttgttttttg tcatccgatc atctacaaaa aaagaaccaa
      181 tcattcctga aaaaaaaata acagaaaaag caaaagaacc gacagatgat aagcaacaac
      241 tagttactca agcaaaaaat ctggctcaaa gctacgatta tgaccaagcg attgcgttat
      301 tggaaaaaga tagtaagaac ggatctactg aaacacaaca gcttcttcaa acactaaaaa
      361 aggagaaaga acaactgatc gactggtctg atcctactca gatctctcat gtctttttcc
      421 acagcttgat cgtggacccg caaaaagcct tccactctca acaggcacaa ggatacaaag
      481 attatatggt gactgttgaa gaattcaacc gttcgatcga acaactttat caaaatgatt
      541 ttgttttagt taatttgaat gatttgatcc aaaaagacga acaaggaaat ctttccttta
      601 acgggcttgc gctacctgaa ggaaaaaaac cattggttct ttcacaagat gatgtcagct
      661 attatgaata tatggataat tccggttttc ctagccgatt ggtcgtatca aataacaatg
      721 aaatcaaaaa tgtctataaa gagaaagata aagaagaaat cggtgacttt gacatggttc
      781 ctctgatcga tgcctttgtc aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta
      841 ccttggccct gacaggttac aacggagttt taggttatcg aacatcaaaa tctcaatatg
      901 gagacaatga aaaaacaaaa aaagagatta aagaagctaa aaaagtggcg gaacagttga
      961 agaaagacgg ctggagcttt gcttcccata catggggtca cttgaatatg actcaagctt
     1021 cactagaaga tgtaaaaaaa gacaatgaat tgtggcagca agaagttgca cctatcctag
     1081 gaaaaacaaa tattctgatt tatccatttg gtgcagacat cagcgactgg cagccttatt
     1141 cagcagaaaa tcaaaaattt gcttacttga agagtcaagg tttcgatatt ttctgtaatg
     1201 tcgatgcttc tacaccagct tggggacaat taggtacgga tttctatcga aatgctcgga
     1261 tcaatatcga tggcattcgt tttcaatctg atttagacgg aaaaaatcct attcttgatc
     1321 cattcatcaa tgtccatgat gtctatgaca aagaagcacg cgaatctgct tgattttacg
     1381 tcatttatga accaaaaaag ccgaactgac attttatcgt cagtttcggc ttttttatgc
     1441 tttatttctt cttcggtgcg attcccttga tcaggacttc tgtccattct gctgtatatg
     1501 gaagcagttt tgcgtatatc tccggcttag aaattccttc taccattgct tgaaaataga
     1561 ggatcaagaa ctcatccgaa tatttcaaat ccacttttcc ttctttccgc cctcgatgga
     1621 aaagatcaag catgatactg aaactttctt gcgtatacgc atccatcgcg tcagaaaatt
     1681 cttttttttc tttttttgta tagtatgtca tcaaatcgat ataaaattgt ttgttcactc
     1741 gattgagata acggattttg ttttcactca tggcaatcag tgtttcttca aatggaagat
     1801 tttccgcaat caacgctttc gcttcttgcc ctaaatcatt cataaatgca aaaaatactt
     1861 cataaatcag cccttccttg ctgccaaaat atttaaaaat cgtcgcttta cttacctgag
     1921 cgttctcagc taccttatcc attgtcactc cagcggttcc tatgtctgtg ttcatcaagt
     1981 caaaagctgc ttgtaaaatc tctttctttt tctcttgtgc tctcttttca aaaccgttca
     2041 tttgtagagc ctcccgtaat tttaaacttt tataatatat atagttcaaa tttattctga
     2101 ggtttattat aactatctat tgagaaagta tcaagctagc aattccttgt ttatgaattc
     2161 attgacagaa atttataaaa agaatattat gtactaaaat aataaaaaaa gttttataaa
     2221 aaggtataaa acctttttat taaactattt aatcttgttt agtatatatt tttagaaaaa
     2281 ggagtggggt ttttatggca gaaatcgtca aagtcactca tttgaaaaag agctttggtc
     2341 gtttcgaagc tttaaaagat gtctctttca ccgtacattc aggtgaagtc gttggattta
     2401 tcggaccaaa tggttctgga aaatccacga cgattcggac acttttaggg attataaaaa
     2461 aagacggcgg aacggcaacg atttttgggc aagatgtatg ggaagatagt ttatctatcc
     2521 atgaacgtct ttcttatgtt ccaggcgata tctctttgtg gggaaatttg actggaggag
     2581 cgatcatcga tctcttcatg aagcttcatg gaaaagggag tgctcaaaaa aaagatgaac
     2641 tgattcaacg tttctctctt gatcctaaga aaaaagcaaa aaactattca aaaggaaatc
     2701 gccaaaaagt gggcttgatt gctgctttgg ctgttgattc ggatctctat ttatttgatg
     2761 agcctacttc tggtttagat cctttgatgg aaacgatttt ccaagaagaa gttgaaaaat
     2821 tgaaagaaca gggcaaatcc atcctacttt cttctcatat tctcagtgaa gtcgagcgac
     2881 tagcggataa agtcgtgatc atacgcgaag gaacactcga tgaagaagga acactcgatg
     2941 aattgcgtca tttgactcgt tcgaccatca ctatagaaac agaagcagag aatagtcact
     3001 tagcacaatt gaacggtgtc catgactttg tgcaaaaagg tcacctcgct caatttgctg
     3061 ctgataacga agctttcgat gtgattttag ctgaagcgtc caaattaggt gtgaaaaaat
     3121 ttgaatctat tccaccgact cttgaggatt tattcatgag acattatgaa ggatgaggag
     3181 gaagcgaaat gactgataaa ttttcacgct ggtccaccct tttttctcaa aaccttagcc
     3241 gtgactggaa aaagatcctg atatggctag tcggtgtcac tgctttttct ggtggttttg
     3301 tccctgcgtt tgaagaattg acttccggac aaggattagc tggattatac gaaaccttgc
     3361 aaaacccggc aatgattgca atggtcggac caactccagc ttcgaatgct gctgagtata
     3421 ctttaggagc gatgtatgca aatgaaatgt tgcttttttg cagtttgttt gctgctgtga
     3481 tctctgtttt acacgtggtg agccaaacga gaaaaaaaga agaacttggt ctgattgaac
     3541 ttattcgttc ctttcgagtc ggacgtcaag caaactcttt agctgtcatc gctgaaagta
     3601 ttctgatcaa tttgctttta gcaatatcga tcagctgtac gatgattttt ttccaagcgg
     3661 attcgatcac tgcagaaggt gcttggcttt ttaggctatc tgtaggtagt atggggattt
     3721 tggggagtgc cgttgcactg tttgctgcac aactgatgcc aagttcagcc agttcgaatg
     3781 gtctgtcttt agcgttttta ggtggacttt accttctacg agcaataaca gatgtagctg
     3841 attcctccct ttcgatgtgg aatcctttag gctggtctta tttgacctat ccatttacaa
     3901 ctaacaactg gattcctttg ctttatttgt ttttgttcag tctaatcttg ataatcagtt
     3961 catttgtttt agaggaacat cgagatttga acaccggcta tatccccgaa cgtgaaggaa
     4021 aaccgtatgc taaacgttct cttttatcct tgcgtggttt attattctat ctaaataaag
     4081 ggatgacaat cagttggctt atcacttttg gtttgatggg ggcggcatac ggctctatct
     4141 acgggaatat gcaacagttt ttagaaagta acgacttaat gaaacagatg tttatccaat
     4201 ctggcgtctc aattgaaaaa aactttacaa gcacgatcat ggtcgtcttg atttgtctag
     4261 ctgctatttt accaattgct agcatcaatc gtttatttac agaagaaacc cacggacgta
     4321 tgaatcagct gttcgcaaca aaaatttcta ggaaccaact ttattggacc aatatgttac
     4381 tagctattct atttgctata ttggcaatcc tgattagttc tggtgcacta gggctttctg
     4441 ctttggcttc gatgaaggat gcgaaagaca tggatctatt gatgtttctt aaagccgggt
     4501 tcaatttcct gcctgccgtt ttagtgttta tcggaattag tggctttcta ttcggatggc
     4561 ttcctcgaat cggtaaactt gcttatatct acttagctta ctcctttgca ctcaattact
     4621 ttggcggaat tttggattta ccagaatggt tttccaaaac agctgtccct agctgggttc
     4681 cacggatgcc aaacgattct tttgatcttt ccgtgttcct tacgttatca tgtatcagcc
     4741 ttgcactgtt cgtccttggt tctattggtt ataggcgaag agacttacta gaaggataat
     4801 aaaaaagcat gtacaatttc atgctagcct ccaaatctgg gtcaaaaatc caacatttgg
     4861 aggtcatttt tttatttaca agtcttctaa ctgctcttta tcaatgatcc agacttctcg
     4921 attctttctt tttacataac cattctcctc taataattta aattttctag ataaagtttc
     4981 gggggtcgta ccgataaatg cagcaagttc tttcattttc attggcagga aaaagtgatc
     5041 cttccctgtc gctttagcta agtcaagcag atagacagcg actcgttctt ctactttttc
     5101 catcatcaaa aaagttgtct gctgctgtac ttttgttaat ttctcaacgt tgattgctaa
     5161 aagtttcaga ctaagggcgg gataatgcag taagagttcc gagaaatctt tttgtctcaa
     5221 tatgcaaatt tccgtttttt ctagtgcttc gccaaataaa gactcatttg gaacgcctag
     5281 caactggctc tctccctcat atccgcccgg ctctattact cgtaaaagct gttcctttcc
     5341 gctagcagat aattgataca ctttcatact gccggaggcc acgatcacta attgaggttc
     5401 tccattcggc gcaagaatct gttctccttt ttccacatag tgatgtttga caagtgcgtg
     5461 tactttttct tgatcttgct gtactaagtg attgaacaaa ggaacaaagg aacaaatgaa
     5521 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
     5581 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
     5641 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
     5701 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
     5761 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
     5821 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
     5881 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
     5941 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
     6001 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
     6061 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
     6121 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
     6181 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
     6241 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
     6301 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
     6361 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
     6421 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
     6481 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
     6541 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
     6601 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
     6661 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
     6721 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
     6781 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
     6841 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
     6901 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
     6961 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
     7021 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
     7081 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
     7141 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
     7201 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
     7261 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
     7321 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
     7381 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
     7441 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
     7501 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
     7561 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
     7621 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
     7681 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
     7741 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
     7801 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
     7861 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
     7921 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg
     7981 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
     8041 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
     8101 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
     8161 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
     8221 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
     8281 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
     8341 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
     8401 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
     8461 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
     8521 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
     8581 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
     8641 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
     8701 attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct
     8761 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
     8821 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
     8881 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
     8941 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
     9001 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
     9061 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
     9121 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
     9181 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
     9241 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
     9301 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
     9361 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
     9421 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
     9481 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
     9541 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
     9601 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
     9661 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
     9721 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
     9781 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
     9841 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
     9901 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
     9961 gcaccagaag agcttatggt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    10021 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    10081 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    10141 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    10201 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    10261 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    10321 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    10381 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    10441 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    10501 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    10561 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    10621 tcagccagac ttctgttcca ccttttgccc aacgttttct gtgtcgatat agcatcttca
    10681 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    10741 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
    10801 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    10861 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    10921 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    10981 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    11041 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    11101 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    11161 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    11221 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatcttcga
    11281 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    11341 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
    11401 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaaatac ataatagaca
    11461 aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca gttccacccc
    11521 attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt cggagacaga
    11581 ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat acacttcttc
    11641 aagtaattct tgtctttttt tcaatttttc atcttcata
//
LOCUS       DAYYNG010000085        11456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000085 DAYYNG010000000
VERSION     DAYYNG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.085"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      87..253
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            401..958
                     /locus_tag="ACHBFA_001820"
                     /pseudo
     CDS             401..958
                     /locus_tag="ACHBFA_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1016..3055
                     /locus_tag="ACHBFA_001821"
     CDS             1016..3055
                     /locus_tag="ACHBFA_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9395297.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEYPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3164..4315)
                     /locus_tag="ACHBFA_001822"
     CDS             complement(3164..4315)
                     /locus_tag="ACHBFA_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9395298.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4469..4999)
                     /locus_tag="ACHBFA_001823"
     CDS             complement(4469..4999)
                     /locus_tag="ACHBFA_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9395299.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5098..5448)
                     /locus_tag="ACHBFA_001824"
     CDS             complement(5098..5448)
                     /locus_tag="ACHBFA_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9395300.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5605..7134)
                     /gene="ahpF"
                     /locus_tag="ACHBFA_001825"
     CDS             complement(5605..7134)
                     /gene="ahpF"
                     /locus_tag="ACHBFA_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM9395301.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7144..7707)
                     /gene="ahpC"
                     /locus_tag="ACHBFA_001826"
     CDS             complement(7144..7707)
                     /gene="ahpC"
                     /locus_tag="ACHBFA_001826"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM9395302.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7851..8513)
                     /locus_tag="ACHBFA_001827"
     CDS             complement(7851..8513)
                     /locus_tag="ACHBFA_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395303.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8697..10343
                     /locus_tag="ACHBFA_001828"
     CDS             8697..10343
                     /locus_tag="ACHBFA_001828"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9395304.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10334..11416
                     /gene="uxuA"
                     /locus_tag="ACHBFA_001829"
     CDS             10334..11416
                     /gene="uxuA"
                     /locus_tag="ACHBFA_001829"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9395305.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         3851 a         2143 c         2169 g         3293 t
ORIGIN      
        1 agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta ggaaaagtct
       61 atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc ctatacaaac
      121 ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt
      181 aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta aaactcggac
      241 gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt ttctttatat
      301 ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag tggattgctg
      361 tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ggtcgctcgg attcacttct
      421 ggttgcacaa tacaaccaga aaacacaaca agcaaaactg atctctatca tgagagattc
      481 atatgtcgat attccaggtt acggaatgaa taagataaat gcagcttact cttatggggg
      541 agttgattta ttaaatcaaa cattaaagga aaatttcaaa tttgaagctc cctattatgc
      601 aagtatcaca tttcaagatt ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa
      661 gatcgatgca gaaaaatctc tagatttaga tggcgtatat ataaacaaag gagagcaagt
      721 gatggatggt aatactttac tccagtatgc tcgatttcgg gaagacaaag aaggagactt
      781 cgggaggatc agaaggcagc agcaagtgat caaagctatc tctcagcagc tgaaagacgt
      841 aacttcaata ttcaagctac ccaaagcggt tgggaaatta cttggtagta tccaaacgaa
      901 ccttccagaa agtgtgcttc tggattgtgg tatggatttc ctcaaagaca tatgttagaa
      961 taaacaggta taaatagtga aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa
     1021 aagaagtgac aagcacggca aaaatcgaac aggcgcttat attgccggcg cagtgatctt
     1081 aatagcagct gcgggtggcg gttattttta ctaccagcac tatcaagaaa cccaagcagt
     1141 agaagctgga gaaaagacag ttgagcaatt tgtccaagct ttgaacaagg gagattataa
     1201 caaagctgca gaaatgacat caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga
     1261 gatcttagat caataccaga atatatacgg tgctgctgat gtcaaaggac ttcagatatc
     1321 gaatctaaaa gtagataaaa aagacgattc tacttacagt ttttcatata aagcaaagat
     1381 gaatacctca ttaggtgaat tgaaagatct ttcttataaa ggaacattag acagaaatga
     1441 tggtcaaacc acgatcaact ggcaacccaa cttggttttt ccagaaatgg aaggaaatga
     1501 caaagtaagc ctgaccacac aagaagcagc aagagggaat attatagatc gaaatggtga
     1561 accattagca acaacaggca aactaaaaca attaggagtc gttccaagta aacttggaga
     1621 tggaggcgaa aaaacagcca atatcaaagc gattgcttcc tccttcgact taacagaaga
     1681 tgctatcaat caggcgattt cacaaagctg ggtacaaccc gattactttg tcccattgaa
     1741 aatcattgat ggagcaacgc cagaacttcc agctggagct accattcaag aagtagacgg
     1801 cagatattat cctttgggtg aagcagctgc tcagctgatt ggttacgtgg gagatatcac
     1861 agcagaagat attgataaga atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg
     1921 tttggagatg gcttttgata aggatcttcg tgggacaaca ggtgggaaat taagcattac
     1981 agatgcagac ggtgtagaga aaaaagttct gatcgaacat gaagttcaaa atggcaaaga
     2041 tatcaaattg acaatcgatg cgaaggcaca aaaaacagct tttgacagtc taggaggaaa
     2101 agctggatca actgttgcga caacgccaaa aaccggtgat cttcttgcgc ttgctagctc
     2161 tccaagctat gatccaaaca aaatgacaaa cgggatctca caagaagatt acaaagctta
     2221 tgaagaaaat cctgaatatc cattcatcag ccgatttgcg acaggttatg ctcctggatc
     2281 tacgtttaaa ataattacag cagcaatcgg tctcgacaac ggcactatcg atccaaatga
     2341 agtgttgacg atcaacgggc ttaaatggca aaaagacagt tcttggggat cttttaaaac
     2401 aacgcgtgtc agtagtgatg tatcacaagt agacttaaaa actgctttga tctattccga
     2461 taatatatat gcggcacaag aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt
     2521 agataaattc atttttggtg aagaccttga tttgccaatt agtatgaatc cagcacaaat
     2581 ttctaatgaa gatagcttta actcagatat cttgctagct gatactggat atggacaggg
     2641 cgaacttcta attactccta tccagcaagc agcaatgtat tctgttttta ccaacaatgg
     2701 cacacttgtc tatcctaaat tgattgcaga taaagagaca aaagataaga agaatgtcat
     2761 cagcgaaaca gcagtacaaa cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa
     2821 tggtacagca cattctcttt ctgctttagg gattccgttg gcagcgaaaa ctggtacagc
     2881 ggaaatcaaa gaaaaacagg atgtaaaggg gcaagagaac agtttcttgt tggctttcaa
     2941 tcttgataac caaggttata tgatggtcag catgttggaa aataaagaag atgatgattc
     3001 agcaaccaaa cgagcatcgg aactattgca atacctcaac caaaattatc aataaaaaat
     3061 tgctttttaa gtacagtaaa aggactgtgg cgttatcgtc acagtccttt tttcaaataa
     3121 acggttttca cgatgtctct ttcacaactt ctttttgatt tactcacact tcataaactt
     3181 tttttgtaaa atatttgaga acaagtggag aaataagtgt acttaataaa atcacgataa
     3241 ctaaaggtga atagtactgt ggttcgatca attgactttg ctgtccaatt tgtaaaatga
     3301 tcaaagccat ttctccacgc gaaatcatac cagcaccgac catcaatgaa ctatgttgag
     3361 aaaaaccggc aatctgagca ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa
     3421 gcgtcaatat agaaataaag agtagttgtt cactaaagtc agaaaaatca acttctaatc
     3481 ctacactgac aaaaaatact ggtataaata ctgcatatcc taaagcctct acattgtggt
     3541 atacttcttc tttaaccttt gtctgactga cagctatccc agcaaaaaaa gcaccaatga
     3601 cagagctaag cccaacaaga tccgctacgt aagacattcc taaacaaata actagcgata
     3661 cgatgataat agaagaattt gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa
     3721 tccatttgac taataagaaa ataaaaacaa aataaattag ctgttcaatc aaaacaagcg
     3781 gtaaagaagc attgctagaa gcttcccccg ttagaaaaga taggctgaaa cttagaacta
     3841 aaacgactaa aatgtcatct accacagaag cacccaaaat tgttgatcct tcttttgtat
     3901 tcactacgtt tagttctttt aatacttcta ctgaaatact gacagaagtt gccgcaagaa
     3961 taatcccaaa gaaaatcgct tcatttcccg aaacttggaa tgctttgcct gtcaaccagc
     4021 caaaaacaac cgggaagatg atcccaagca aagcaacgaa cataccaggt cggaaatatt
     4081 ttttcaaaag agataaatca ctttccagac cagctaaaaa cattaataag aacacaccaa
     4141 tctccgaaaa gtcatggact agaatatcag gtttgaccca attcaatcca gcttggccta
     4201 gcaaaacacc aacaagtaat tgtccaatca ctgctgggat acctaatctc cgcgagatat
     4261 gtgagccaat cgttgttgaa atcaatatca ggcataaaat accaataaat tccattctca
     4321 tcattccttt gtataaaata tagcccgaac ttagcataac atacattatt gaaaaaatag
     4381 gaaaatatac tcacaaaaaa accaacaatc caaaagattt ttttggattg ttggttttta
     4441 tacggtctat tttaataact aaaaatgatt aacgtatagt catttttctt attctgatga
     4501 aattgataat ctcctggatg cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa
     4561 gcatcaaact tttgaatgga ttgaacaata agacgaatcc gccaatcaac aacagaatac
     4621 tactataaac actccaactt ttagcttttg tattcatgct gaagaacaac tggaacaagc
     4681 tgttgatgat aacagctaat ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa
     4741 aagcatagac gatcagagct aagatcagga aaaaaagacc actgaacaga ccgatactcc
     4801 aagaacctgt tttcctttta aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa
     4861 caagataggc ggtgatcaga taaccgataa aatgaaaaac agcagaaggg tttagcacaa
     4921 tcgcaatccc agaaataata taaacagcag ctcttaataa ggcgtatttt tgaatatttt
     4981 gtttaatcgt ctcaaacata taagaaaacc tcctttgact tattttaacg tgaactagtc
     5041 attttaccaa attaaatgtt acaaaatgag cgaaggagaa gaatatctct ggaaaaatca
     5101 acttctgagg atcttttcca tttttttgcc ttttgctagc tcatcgatca ttttatctaa
     5161 ataacgaatt tctttcatca agttatcctc gatgtcttct actcgtacac cacaaacaac
     5221 acctgtaatc aaggaacgtg aaggattggg gacaggggct tccgcaaaaa attgttcgaa
     5281 agtcacttgt ttttgtagtt gatattctag ttctgtttgg gaataacccg tcagccaaca
     5341 aatgatttca tctacttcct cttttatacg atcttttctc tccgcttttg ctacatacat
     5401 aggataaacg cttgcaaaac ttgtcgtaaa aatacgattt tgcgccataa tcagtcacct
     5461 ttcttttttt caaattattt actacttata gtatataaga aaaagagaag aaaaagcgat
     5521 tattcaaaaa aacaaaaagg attgtgacaa aattcttgtc accatccttt tttccgctct
     5581 ctgacttttt acaatcttca tagctcaagt gcgcatcaga taatcgaatg ctcctaaagc
     5641 tgcagtagct cctgaaccca ttgaaacaat gatttgtttg tatggactat ctgtacaatc
     5701 accagctgcg aaaacgccgg ggatattcgt tgcgccattt ttctcggtca ctatttcatt
     5761 tcttggattc atttcgatcg ttccatctag ccaatccgta ttagggagta agccgactag
     5821 aatgaatact cctgaaacag ctagtgtatg catctccatc gtacggcgat cgatatagga
     5881 gagagattct acctgattcg taccgctgat ttctttcgtc gctgcatgtt tgataacagt
     5941 gacattcgca agagagacta gtttatcctg taagatttga tctgctttta attctggtaa
     6001 gaattctaga acatatacat gttttgctaa cccagccaaa tcgattgctg cttcaacacc
     6061 agaattacca ccaccgataa cagcaatctc ttttccttta aataaagggc cgtcacagtg
     6121 tgggcaataa gcaattcctt tatttttaaa ctctttttcg cctggtacat tgatagaacg
     6181 ccagtgcgca cctgtcgcga tgatggcggt ttttgcttta agtgatatac cgttagctaa
     6241 gccaatatta ataaaatgtt gctctttatt taaggaaacg gcctgttgat tcatcataat
     6301 gtcgataggg taactacgca catgactttc tacttgctgc atcaattttg gaccttctgt
     6361 gtaagaggta ccaatcatat tttcaatccc tagtgtttca acgacttgtc cgccgaaaga
     6421 atcagcgaca agtcctgtac ggattccttt acgtgctgca tagatagctg cactactagc
     6481 tgctggtcca ccgccaatca ctaatacatc ataaggttct ttttcaaaca actcttcttc
     6541 aatcaaaggg ccagtgattt tttcaacaat ttgttcgatc gtcatgcgtc cgctactaaa
     6601 ttcttctcca tttaaaaaca cagttggcac tgccatgatt tttttatcat cgatttcttt
     6661 ttggaacatc ccaccttcaa tcatcgtatg agtaatattt ggatttaata cagctaatat
     6721 attcaatgcc tgtacaacgt cggggcaatt atgacaagtc aagctaacat aactgtcaaa
     6781 atgcaatgtt tgtgttattt ttttgatttt aagactcaaa gattcactga ttttcggtgc
     6841 ccgtccacta atttgtaaca tagccagtaa aaaagaggaa aattcatgac cgagggggat
     6901 accagcaaaa acgattccac tttctttgcc aactggattg atagaaaaac taggtgtgcg
     6961 catcgaatct cttttttccc aagagatttt aggagacatt tctgctattt ccttcgtaaa
     7021 ttcagctagt ttttgactat tttcactatt atccaaacta acagaaaaaa caataggaga
     7081 ttcaatcagt gctaaatatt gttcgagatt ttgtttagtt tctttgtcta acatatccac
     7141 cacctaaatc tttccaacaa gatcaaaact tggtttcaat gtttcgccac tttctttcca
     7201 ttttgccgga caaacttcac ctgggtgggt acgtacatat tgagcagcgc gaatcttatc
     7261 aatcagtgca ctagcgtcac gaccgattcc atctgcattg atttccatag cttgaacgat
     7321 tccgtctgga tcaataataa atgttcctcg ctgagctaat ccttgctctt catccaaaac
     7381 atcaaaaata cgagaaatct gatgagacgg atcaccgatc atcacgtatt cgattttacc
     7441 gatagcatct gatgtatcat gccatgcttt gtgagtaaag tgggtatcgg tagagcaaga
     7501 aaagacttca acacctaaag atttcaatgt actgtattgt tcttgtaagt cttctaattc
     7561 agtaggacaa acaaaagtaa aatcagctgg atagaagcaa agaatactcc agttccccat
     7621 aagatctttt tcactgactt ctaaaaattc tccttgatga taagcatgtg cattaaattc
     7681 ttcgattttt ttcccaatca atgacatagt aaaacctcca ataataatta tttatttata
     7741 attattgtaa tttagttgga gttgcaaagt caatggatat tttgttattt aataaagatt
     7801 actcaaaaac gaaggttaat tataaaaata gaagaggtcg caagaaaatg ctactgctgg
     7861 aaatactcgg gataacgttg agtaacagtt tcctgttctt tcagcatcat gtgtaagtgc
     7921 aatcccgttt gaaatcgagc ttcttctggt tttttatcaa ctaaagcctt gagtaaatca
     7981 taatgttgtt ccatgatcgt ttcccatgga agcccctgaa tggtcactcg taaccagcgg
     8041 aaacgttcta aatgggtatt atggtcatct aaccagttcc aaacttcttt tcttcctgca
     8101 atttcgaaac aagcttcatg aaataaatta tctgcacgga aaaaagcgcg ttcgtcattt
     8161 attttgactg ctttttcttg ttcttctaaa atcgtttcta gcactattcg tgtttgtgtg
     8221 tccaatttgg aacaacattc aatcatgatt tgctcttcca actgttctcg gacaaagcga
     8281 gcattttctg cagaaacaag attaattttt gaaacatacg taccactttg aggtatcgta
     8341 tacacgagtt cttgctgacg tagttgaatc aatgcttcgc gaatgggagt ccggccgata
     8401 gcaagatgtt cctccagtgc cttttcagat attttccttc caggttctaa gtcagcatag
     8461 ataatttgtt tacgaatcgc ttgataagct tgagattgca agctgctttt catctttatt
     8521 cctccaaaat catttctatg aacgtagtat aacagaagag gtctgaaaaa caatctggta
     8581 tgtcacatta actagtaaat ttgtataaat agtaaaattt tggttgacat agcgattttt
     8641 attgcataaa attcacttga atacaaaaac taatatgtta gtttaggagg ggaaaaatga
     8701 ttgatttaaa caactggagg aactttatcc ctgaattatc tcaaaaagaa gtagaaatac
     8761 ctcattttga ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc tggatccatt
     8821 tcggtggcgg aaatctttat cgaggatttc atgcagaaat cgctcaacgt ttagcggatc
     8881 taggcgaatt gtccactggt gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa
     8941 gggcttacca agattttgat gatttgattc ttgaagtggt catgccaagc agtggagagt
     9001 tcgtcaaacg tatattagct gcgacagctg acagtctgta ttgtcatcca gattgttctg
     9061 aacactttga taagatgatt cgacactttg aaaatcctag tttacaatta gttacttttg
     9121 ctattactga aaaaggttat cgattgaaag atacaaataa taaatatata ggaccgattg
     9181 cagtcgatat aaaaaatggt ccaaagaagc catcacatac aatgagtatt cttgctgcgc
     9241 tactttataa acgtttttta tctggagctt ggccgattgc acttgtctct acagataatt
     9301 tttcacaaaa cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa
     9361 aggaaggtta tgtacaaaat acatttatcc aatacttatc gaatgaagaa attgttgcgt
     9421 ttccatggac gatgattgat cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt
     9481 tggcaaaaga gggcttttca aatatgtcga ttttccaaac cgcaaaagga aataatattg
     9541 ctccatttgt caatacagag gacatccatt atttaatcat ggaagatcat tttcccaatg
     9601 gacgtccgaa attcgaaaaa gctggagtat tcttgactga tcgtgcaacg gtagataaag
     9661 cagatacgat gaaggtgacg acttgcctta atcctttgca tacgtcttta gctattatgg
     9721 gttgtttact ggggtatagg agcatagctg aggagatgaa agatccagat ttatcgctgc
     9781 tagtttctga aatcgggtac aatgaaggcc tcccagttgt ggagtctcca ggtatcattg
     9841 atccaaagat ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca aatattcctg
     9901 atatgcctca gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt tatggtgaga
     9961 cgattaaaaa atatatggaa atagaagtta aagacccgca gacacttgta ttcataccat
    10021 tgactctcgc tgcttggctt cgatacctta ttggcattga tgatcaagga aaaccattca
    10081 tcccgagtcc agacccacta ttgacagact ttcagcataa gctttccgat attcaactgg
    10141 gtacgcaaga tacatcacat attcatgaga aaatggctcc tattttgtca aataaagaaa
    10201 tttttggtgt agatctttac caagcagagc ttggcagaaa aatagaagaa atgttcatca
    10261 agatggtagc aggaccgaat gccgtacgaa aaacattaca ggattactta caagaacatg
    10321 gaggaaattt atcatggaaa tgacttttag atggtacgga tcacaggaag aaaagattac
    10381 cttagatgaa atcagacaga ttcctgcggt caaagggatt gtaggcacat tgatggatat
    10441 accagtagga gaggtatggc ctagggagag gatcaaagaa ctaaaagaag aaattgagtc
    10501 tcgtgggtta acactaaaag taattgaaag cgtcaatgtg catgatgcca ttaaaattgg
    10561 attaccttcc cgtgagcagt atatcgaaaa ctataagcag acaatccgaa acttggcaga
    10621 atatggaata gaagtcatct gctataattt tatgcctgta tttgactggg tgaaatctga
    10681 ccttgactac cgcttagaag acggctcatc cacgttagct tttatatctg atgatattcc
    10741 agatgatcca agagaaatcg tcgcgcgcat cgaacattcc agcaatgaat ttgaattgcc
    10801 gggatgggaa cctgagcgct tagctcatat caaatccttg cttgaagcct atgcatcagt
    10861 agacgaagaa aaacttcgag aaaattttgc ctattttctc caatcaatca tcccaacatg
    10921 tgaagaggta ggggtcaaaa tggctgtaca tccagatgat ccgccatact cgatttttgg
    10981 attacctcga atcgtaaaga atcgagaaga tttagattgg ttgtgccgca tagtagacag
    11041 cccttataac ggaatcactc tttgtacagg gagtattgca gaagatccag aaaatgatgt
    11101 atatgggatt ttagcagaat tttgtcaaag agatcgaatc ccatttgcac atgttcgaaa
    11161 tattaaattt ttagctaatc gagatttcta tgaggcaccg cataaatccg aatacggttc
    11221 attagacatg taccgcatac taaaaacaat gtatgacaac ggatttaacg gatacatccg
    11281 tcctgatcat ggaagaatga tctggaatga gacaggaaga ccaggatacg gcttatatga
    11341 tcgagcatta ggtgctgttt atctaaatgg attatgggaa gcaatcgaaa aaggaaacaa
    11401 aagtaaagat aattagtgaa aaaaactgta attttattat gtaaataaga aggatg
//
LOCUS       DAYYNG010000086        11377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000086 DAYYNG010000000
VERSION     DAYYNG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.086"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            264..470
                     /locus_tag="ACHBFA_001830"
     CDS             264..470
                     /locus_tag="ACHBFA_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM9395306.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(672..1673)
                     /locus_tag="ACHBFA_001831"
     CDS             complement(672..1673)
                     /locus_tag="ACHBFA_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9395307.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1678..3591)
                     /locus_tag="ACHBFA_001832"
     CDS             complement(1678..3591)
                     /locus_tag="ACHBFA_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9395308.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3759..4265)
                     /locus_tag="ACHBFA_001833"
     CDS             complement(3759..4265)
                     /locus_tag="ACHBFA_001833"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9395309.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4425..4865
                     /locus_tag="ACHBFA_001834"
     CDS             4425..4865
                     /locus_tag="ACHBFA_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9395310.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4891..6048)
                     /locus_tag="ACHBFA_001835"
     CDS             complement(4891..6048)
                     /locus_tag="ACHBFA_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395311.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6051..6407)
                     /locus_tag="ACHBFA_001836"
     CDS             complement(6051..6407)
                     /locus_tag="ACHBFA_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395312.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6705..7598)
                     /gene="sgrA"
                     /locus_tag="ACHBFA_001837"
     CDS             complement(6705..7598)
                     /gene="sgrA"
                     /locus_tag="ACHBFA_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM9395313.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            complement(7794..8633)
                     /locus_tag="ACHBFA_001838"
     CDS             complement(7794..8633)
                     /locus_tag="ACHBFA_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9395314.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8818..9705
                     /locus_tag="ACHBFA_001839"
     CDS             8818..9705
                     /locus_tag="ACHBFA_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM9395315.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10299..10994)
                     /locus_tag="ACHBFA_001840"
     CDS             complement(10299..10994)
                     /locus_tag="ACHBFA_001840"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM9395316.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGTDYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(10978..11376)
                     /locus_tag="ACHBFA_001841"
     CDS             complement(10978..11376)
                     /locus_tag="ACHBFA_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9395317.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3403 a         2262 c         1706 g         4006 t
ORIGIN      
        1 taggatacat cgaatttttg gggaaattct tttgggggta tcctaaatct tttctattat
       61 attcccatat tttctattat tggtctagac gtatgatggt aaactttgta cctatctgat
      121 tgctgaatca ttaattgctt atcattagcc atctttgttt caagttcgct agatgatcat
      181 tttattcaca tcctaacaaa cttgtcacat ttatagattc ttaatctatt tatcatacaa
      241 ttataaaaaa ggaggtgctg cttatgaaag ctatccatat ttctttgctt tttttcgcta
      301 gtgcattgat tttatttgct attcatcttt gttcccagcc ttatatcaat aacaacggaa
      361 tattagtgga accagcattc ttttgcttac cactcggctt tttgtccctt cttgcctccg
      421 caggcttcgg atttgttgct ttttttaaac gatcaaaaca acaaatctga cttataaaat
      481 ttacgtaaaa gagatcgtga caataatcgt gtgatattcc caaatttcga ataaaaggtg
      541 ccccttgcca tctctaactc ctcagtcaca agttacaata ttcataaacc atgaaaagct
      601 tgtttattga aattgctcct tgtgacttgg agctaagtga cagattacaa cctcttttgc
      661 atcataagac tttactcctt gaaaaacatt actaactttt gatatgtttc ttgcgcttgt
      721 ttattgctga aatcaaactg atattcatgg ttcaaaacag catttttctc cgcataatct
      781 acttttgtga ctttcacccc cgctttctca aatacagaag ttacttcttc catttgtttc
      841 gtaaacgtat ttgtatttcc atccgttaag aatgtgtttt gcgtccaacg atccgcatgt
      901 ctgaccaagg ctaaactttt gaccttctct gattgttcaa agtccttatt atcaaagtaa
      961 gcccatccac atttttcata aagaaagcta gagattttat cacttacttg agtaagtgca
     1021 gaaggatcca acagaccaga caaagaaata aacttcgtaa tctgcttgtt ctccaaagtc
     1081 ggatcaagat gatcagcttt tttaatttct tcattttgaa gtgctaacaa atattctcca
     1141 ctaatttgtg cacctgccga atttccacca atcgaaatat tcgaccaatc tgcttgatct
     1201 tttatctctt ttttagctgc ttgaatggct tcgttcaatt ggataactgg tgtaggatgt
     1261 tttgcttccg gtgatcgttc ataatctata tttacgatga tttgctgcgt gtctgaacat
     1321 agatattcca aataatcacg acaatcattt ttatcccctc ctacatatgc gcctccatgc
     1381 atccagaaaa gaatcggtaa ttttttcttt tccactttcg gtgtgtagat atccatatta
     1441 ttatttttgt actttgaagg ataggatatt tcttttttta tatcgatttg ttcgattttt
     1501 tgctggaaat catctggtcg ggcatatact tgttcttttt cacttgtgtc aaattgtttt
     1561 ctaacaagca agctaatcgg ctttggtgtg atttgattaa tcaaaaatac agtgactaaa
     1621 ataactacga tgataactgg tactgcaatc actattcgct tgaaccattt cattcattta
     1681 ccctctcttt cttcgccaaa tccatgtgat aatcgtgcta acagataaaa atccacctga
     1741 ataaatgaga agcgaatgtt tcttttctcc agttttcggc aacattcttt tttttgcatc
     1801 tgtctttttt tgtactttca ctgtcatctg aatcggttgc gtatcatcac ttgattgatc
     1861 ctctcgaggt tcattctttc ccttattgtc tgcattctct gtcggatctt gcttttttgg
     1921 tggagctact gggatttttt tgtaatagaa aataatagtt ggtatcgtct cattaaaaat
     1981 cccttcagtc ggtccttcaa catttgctaa ttcgtatcct tcgaatgtct tttgctctgt
     2041 catataattt tctccataag ctccgtaaag ctggatttcg tcctggtctt tgagtagact
     2101 tttattgttt tcatccacat actttacgtt tatctgatca tggaacacta ctttcaaaaa
     2161 gtccgttaca tgtttcgtgt aggcagcatg gtcaacttcc tgtgcctttc catgccctgc
     2221 tccttctact aataaaagat ctttataccc tcctgataac tcatataatt tttgcgcgtt
     2281 agacacaggt actacatcat ctgctgttcc atggataatc aatttaggca atgaatctga
     2341 cattccaatc gtaggtagag cagcttttgt atccaaatgc atctcctgat caaaatagta
     2401 ttgtttcaaa aggcccataa ataaatcttc atgctcttga tccccaatga tgtctaactg
     2461 attttttatg gcatccggta gtgcgggggc tactgtctgg ctgtacgtaa aacgtagttg
     2521 ttcatcaatt gatgcaaatc cgcaattttc aataattcct ttgacattga caggaatatc
     2581 tttcgctaat acagacatca tcgtagctgc tcccatcgaa ccaccataaa gtaaaatctt
     2641 ttgagatggc cgctctcgaa cttcttgatt tatccagtaa aggacgtcat ctgactcata
     2701 ttgtccatag gtcacataat cgcctccgct attaccagtc gctctggaat caacaaataa
     2761 taaattatac ccggccttat aaaaatcatt gtattcttct gtaacgatcc cgttattcca
     2821 attccctcga aatccgccat ggatcatgac tgttttatct gaaccttgat cgacataata
     2881 tgcatataca tccatcattt tgccatcttg tttgtttagt acagtttgat gaagttgttt
     2941 tgtatttgga tagaagcctg tgttttctgt aggcgttgtc ccatcatctg tacctccaag
     3001 catcgacatc aacacactgt tttttgcaaa agcgatatcg tacaatagat aagtagcaat
     3061 cagatgtttg ataatctctt tatctacacc ataatcatta acagcaagct cagtgactaa
     3121 taactctatt aattttgact tgatgtcttc tgttggtcca ttaagtattt ctgtcatctg
     3181 tttaccgaag taattcaaat attctttacc attgaaattt tcacctttac cgatatcatc
     3241 attttctgtc ccattctctt tgtcacttac tttagcagaa gaggattcgt cgtttgacat
     3301 ttttgtagct gttgcttctt ctgtttgttg gttatctgtg gtagcggtcg tttcttgatc
     3361 tgtgctagta acagatgtct caaccgttgt ttcagtattt gtttctgttt gtgaagtact
     3421 cgatttcgtt tcatttccag catcagtagt ttctgtggtt tctacctccc gagattccga
     3481 ttcggaagaa atgctctctc catatactgt ccattgcggg acgcttgtac ttacagcaaa
     3541 taagatgacc aacgctccct ttaatacttg ttttgttctc aagctattca ctccttttta
     3601 tgtttctagg agaattatat gtaaacgcaa acatttttta tacaaaaaat aaatggttta
     3661 tcccataaaa atagaacagg attattttct cattttacgt aataaagcag aatggataaa
     3721 ccagtatcca ttctgctttt tttaaacgaa gaaacaactt attgctccaa aaatgtcaga
     3781 aatcgtttat cccaaatttt ctcgtaaaaa gcgattgttt cctttgcttt cataaaagga
     3841 ttatcataag ttgtcgttgc tcctctacac atcataagct ggtatcctaa tccatgagca
     3901 aacttcacag tcgtatctat acaatactct gcttgtgccc cacataattc gatcgaatga
     3961 cagtccaatt cagttaataa aacactcaaa ttagtcttat aaaatgaatt cgcatgggtt
     4021 tttctcacaa aataactgtg ctcaggaata gataattccg acaatagctc ccatggtttt
     4081 gtttgtggca ctaattgagc atcttcgtgt tgaacgaaaa taactggttt cccaatcgtt
     4141 tcgtatgttt tgattctttt attcactaat gctgtcactg tttctaattc gtgtatcggc
     4201 gaagaatcaa aacatacccc tttttgtaaa tcgatcacta ctattgcgtc ggctgtgttt
     4261 gacatacttg tgccttcttt cttttttctt gatttaatag tatttcttat ctagctagtt
     4321 ccattatacg tgacaacatt ttcttatttt acacctctaa atggcacttt tcgactattt
     4381 cttcatgtta ttatttcata aatacttagc tattggagga atgtatgatg attaaattgt
     4441 atacaggttc tggctgctcg tcttcaagac gcgcaaaacg ttggcttatc gtccatgata
     4501 taccgtttat cgaacagaac ttaattcgtc aaccactaac caaaaatgaa tttatccact
     4561 tactaaaatt gacaaacaac ggcgtgaccg aaatgctctc tactcattct attagttata
     4621 aaaatcttgt tcacagaata gatgaactca gtttgagtga actatatatt gagattcgta
     4681 catccccttc tttattgaaa aaaccgatta tctacgacca atctgcaaaa cttttgactg
     4741 gatttagcga agataaaatc aggatgttcc tttctaaaac taaaagaaag caagagctca
     4801 acgaactctt gctttctggt catcttgtcg attttaaaga aaataatctt tttgcaatgt
     4861 cttaattttg gcataaacaa tctgcttttc ttaattaacg aaatccaatt tttccaatat
     4921 ggatctattt ttctcattcc tgatttttat gatttcagct ttcagacgcg cccaatcatt
     4981 ttttgtaggt tgtccattcc agtaaaatat ctgtgtattc gtttgatcat atgccttttt
     5041 gtcgatagga taatcgctaa tgacaatatc aggtaactga tcagcggatg cactatattt
     5101 tattgggcgt tcagtcatag ataatatcct tttctttgcc cataattcct gtgttttacc
     5161 atactttgat tgaatataga atatgacagg ttgttcgtgt tttataaaaa tatccctgac
     5221 taacatacag tattgaaaaa tcaaccgtct gttcttttca taataagtat taagctctgg
     5281 aattctgcga gctaccccac taaaaaactg ttgcatgatt ggatacatca ctggaaatat
     5341 atgctgaaaa tctctgtctt ctgctgattt tccaaaagca tccaccttct tttttgtccc
     5401 aaaaaccctt gaatacaaat ggatcattgc caaattgaca tatagataat ttttttctga
     5461 agttgtaaaa caaatcttga agtttttttc aaaattttct acaatagacg attcaatcca
     5521 gccccaactt tcttgtactt tatctggagt atccaacaaa tcttcttcca aataggtgtt
     5581 cattacacca aacataaaat aaaggtaagc ttgctctgtt ggagtgatgt gattcatcga
     5641 ccagaagttt gcttcaaaaa aaggctctag tatgtaatgc gtaaactctt cttttgttat
     5701 caatggatgc acaaatttgt tcatctcttc tgggacatac gtaatcgagt ggccttgttt
     5761 cattcgttgc aacgaaattc ttagtaggct gatccatttc tttctatcta tactacgcgt
     5821 atatggcgca tgtttcgcaa atttttctaa tagttgacgt ttatttttta tacttgataa
     5881 tatatcagaa tgattttcgt cgaatgactg ccaatacatc aagtgaaaaa agtatcggat
     5941 acggtattct tctccttcta tcggattacc ttttttcaag ctgcaagata aatgatagtt
     6001 tttcaaaata gtattaagtt ctcttaattt tctttgaatc gtacgcaaac ttatataatt
     6061 ttttgactaa gattttctaa ggattgataa gtacctttgt aaagtgaatc cagtaaaata
     6121 taaccaatcg ttccctctaa aagtatagaa taaactttac tcagtaaaaa ctgagaagaa
     6181 aactcgactt ttatcatctt gttttctgaa agaaaagata atttatattc tggatacctt
     6241 tcgcaaaacg attctgcttg ttgaatcacc gaattcatct tagccagggt gtagtttaga
     6301 tctaaagcga tatccttcac cacatataat ttctcgtctc tttcataaca atagttcaaa
     6361 aattgatata cttgcctttc attgatatca ataaattctt caaacaaagt acatactcct
     6421 ttttatctat tatttagact tattccgaca gctacatgaa taacgtacaa atagattcgt
     6481 tactgatttt tcagagatac taccatgcgc agtgtgcatt gtcaattatt cttgctctta
     6541 ttttccattt gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa caaaaataga
     6601 tctggcaaag ctacaacttc gccaggcaat acagcactct cgaatgaaat attcactcaa
     6661 aaatctatcg ttttattcaa ttttagatct gttgctttta ttctttattt cactcgatgg
     6721 aagagagaac gaaaatagct tgctcctaca atcaaaatga gtaacacacc catccatgta
     6781 attagcttgt tcaaggttct actaccagtt tctggtaaat cgctttttct attatctgtt
     6841 tctttcgctt tttcttgatg atcaattcca cgagaaacaa aatcaggctg ttttagcaca
     6901 ctttttaggt tgctccctgc atttctcttt tgttccttag ttggtatgat aggtaccaaa
     6961 tcaagttcat tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactt
     7021 tcactactcg tactctccgt actgctttcg ctgctcgtgt tttctgtgct actttcactg
     7081 ctcgtactct ctgtactgct ttcaccgctc gtgttttctg tactgctttc actacttgtg
     7141 ctatccgtac tgcttggcgg tccaacaact gtccaactgc ttggtttcaa attctccgtg
     7201 atcgtcatct gtccatcata aaaaacaatc aaatcaactt tatctttact cttgatctca
     7261 tcaatcgata gttctgcaga aatcattatt gtatactcaa taccagcggt ccattgtacg
     7321 tcttttaaat aaacttcccc aataactttt tctgaacttc cattaccatc ccaaccatat
     7381 gttagaacag catgtacatc actagcattc acgcttttgt ttggtttgac tggaatagta
     7441 atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt tcacagtaaa tgaaagattc
     7501 tcatttgccc gttcatttgc agaagtactg attccaatca ttgctgcaat tatactcagc
     7561 ccaagaataa caaataatac ggtcgctgtt ttcttcattt ctatcactct ccatataatt
     7621 accttctatt tgataaagga attatataaa atgaaaatga tgagaacacg acagtcagtt
     7681 gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat
     7741 caatgacaga cttaaaaaag caagaccgat atcagccttg ctttcttcat cttttattga
     7801 taaacagcag tgtatttctt catccgcttt gttttatatg catagagtaa caaaccaaca
     7861 atcaagacta acaagaataa tgtagttgat atagatgctt cagccccaaa gtttccccca
     7921 gtaattattg tattttgcga ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg
     7981 ctgactggta tacccaaaat agctccttgt gtccaattcc aagtaatatg agcaccaata
     8041 gccgtaaata aacttcctgt actgtagata agtgcgctga aaaagcaacc tgctaaaaag
     8101 atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat
     8161 gcagcagatc caatcattgc tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag
     8221 ccacggaata aaagctcttc tgatgccccc actgcaataa ataaaataaa actcgttatc
     8281 agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg cccccgtcaa atataatgga
     8341 ctagtaataa gcagaaacac tccaattcca acaacgagac ctcccgctaa atacccccag
     8401 tcttttcctc gaacagaaac accataatct tctattcttt ttccttcgac cttactagca
     8461 agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg taccggcaat tgctccaatc
     8521 cattcactat ttccaataaa catatccgca agtgcattat tgacaagaat tcctaaaaca
     8581 aaaccaacta agactattaa aacagatcca atcaaccgaa taatcctgat catctttcat
     8641 ccctccttta tattaagttt aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa
     8701 tgttctccat catagagaga atagattttt acgaactaag aatatctaca gcaaccttac
     8761 gaaaatgaaa gactgtctcc ctaattcttc tgttagactg tttttaataa ggaaaggatg
     8821 ggaaattttt tgaaaaaaca actactaatt atgtgccttt cagcatgtgc aattttacta
     8881 ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt ttaaaggtgg aacacttaca
     8941 gttcaggatt tttatgataa cccaaaagtt aaatcaaata atcaaacact attaaaaaaa
     9001 atgattgtct tcaaagcatt tgatgatgta tacggaaaag agatttcttc aaaggaagta
     9061 acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa actacaagga acaattaaaa
     9121 gcagttggcc aaacagaaga gacatataag aaactcttta aacagatgct ggctttccaa
     9181 tacggtttga aagccaatgt aaaattaaca gataaagatc tagacactgc ttggaaagaa
     9241 ttctatccag aagttagtac acagatcatt ctattttcga ctaaagaaga agcagataaa
     9301 gcaaaaaaag aagcaaatga aggggaaaat ttctccaagc ttgttcaagc ttatggaaaa
     9361 aataaaacat taaaggaaac tgatggaaaa atgaattttg attcaaccaa tccagagatt
     9421 cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt
     9481 cctgtaacag atccaacgac ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa
     9541 caggataaag gttctaataa agacaaatac aaatctgagc tagaaaaaat tgctaccgaa
     9601 gcaaaaactc ttgatacgga attcatggat aaaacaatcc gtaaagtgat gaaaaaagac
     9661 aatgtaacta ttaaagatcc ttatgtaaaa aatatattca aataaacaaa tttgacccaa
     9721 ttggggatgt ggcaaaattt gagaagtgat ttttggaaat tccttcttat ttttgaggca
     9781 aaccactttt gccacaactt catcggggcc tttttttatt tgtaccaaaa caaatcactg
     9841 gatttgttac aatcatgagg atataattta aaaaagtttt ttatttctat cacatgaatc
     9901 aatgcgccat taagtagatt ctttcatgct tccctatttt ttcacttgcc gtatcgcttc
     9961 aaaaacaact atcataaact caaactcgac tgaaaagtta ttttaaaaaa gactaaaaat
    10021 atcccttctg gacttgttct tcttattctg ttatactaga gaaacacgaa agatattttt
    10081 atgcgacact tttcatttgt ttttatgaaa tttattcaaa actggcgtaa cttcagagtt
    10141 acaaggatgt attagaggtt agggaataca tcgtttaaat gagaaataag taatagttag
    10201 taatagtcac ccaaacttta aattaatgaa tattgaatcg tcagatagcc tcctcgagtt
    10261 ttcgagaagg ctatcgttta ttttatggag aaattgattc agaagtaatc agcgaccttt
    10321 tttagttcac caatcgtttc tgcttggaaa gtttgcaccc aatcttcttg gaagcccctc
    10381 actgctttat caatcgaggg catctctaat acttgatcta gtagctcttt acctaacgtt
    10441 atcgcatgag ctccagatcg aatggctgta gtgacttgtt cacaattttt gaaactagct
    10501 gctaatattt ttgttggtct ttgcgtacgt tcgatctcag tccgcaacga atcaatcact
    10561 ttcatagcat caatgccttg attttgcatc ctgttataat aaggtgcaat ataatctgtt
    10621 ccattattaa tagctaaata cccttgtaat tctgtgtaaa tagctgtacc ggtaatcaaa
    10681 taaccttctt ttttcaactt tttgatcgct tgaaggcctg cttgattcac cggcaccttg
    10741 ataaacgtat cttttcccag ttcttctatg ataactcgag catcctctat catttcttct
    10801 tccgttcttc ctattgtctg aatatggata ggaatctgtc cgattatttt tttgatttct
    10861 ttcatatgcg agaagaagtt tgacgctcct tcttttttca gaattgtagg atttgttgtt
    10921 acgcctgtta aattcaaaaa ctgttgacaa gaataaatac tttttgtatt tgctgtatca
    10981 agcatgattt ccatatagct ttcccccatt ctgatcctct tgatcgattt taatgattgt
    11041 cccctttctt aaatgcggca tttccttatc ttctacatac attgttcctg gaagttccgg
    11101 ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat tcattcaaat tagtttggac
    11161 aacatctcct actgctgtga ttacatacat ttgtttatca aaacagagct tcatatcaca
    11221 agttatttct tttcttacct gattcaattc aatcttataa caataatctg ttaatgtttc
    11281 cggtgcttca tctccaaata aaatcattat tttttctttc ctgaatactt ctgcttctat
    11341 accaacatct gttacaaccg tttcaaagat cgtcatc
//
LOCUS       DAYYNG010000087        11368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000087 DAYYNG010000000
VERSION     DAYYNG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.087"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..285)
                     /locus_tag="ACHBFA_001842"
     CDS             complement(58..285)
                     /locus_tag="ACHBFA_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9395318.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(388..2976)
                     /locus_tag="ACHBFA_001843"
     CDS             complement(388..2976)
                     /locus_tag="ACHBFA_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9395319.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(2996..3655)
                     /locus_tag="ACHBFA_001844"
     CDS             complement(2996..3655)
                     /locus_tag="ACHBFA_001844"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9395320.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="ACHBFA_001845"
     CDS             complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="ACHBFA_001845"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM9395321.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4428..5294)
                     /locus_tag="ACHBFA_001846"
     CDS             complement(4428..5294)
                     /locus_tag="ACHBFA_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9395322.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5403..6044
                     /locus_tag="ACHBFA_001847"
     CDS             5403..6044
                     /locus_tag="ACHBFA_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9395323.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="ACHBFA_001848"
     CDS             complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="ACHBFA_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM9395324.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(7485..8384)
                     /locus_tag="ACHBFA_001849"
     CDS             complement(7485..8384)
                     /locus_tag="ACHBFA_001849"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9395325.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(8386..9183)
                     /locus_tag="ACHBFA_001850"
     CDS             complement(8386..9183)
                     /locus_tag="ACHBFA_001850"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9395326.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(9161..9865)
                     /locus_tag="ACHBFA_001851"
     CDS             complement(9161..9865)
                     /locus_tag="ACHBFA_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9395327.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            9996..10571
                     /locus_tag="ACHBFA_001852"
     CDS             9996..10571
                     /locus_tag="ACHBFA_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9395328.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            10689..11366
                     /locus_tag="ACHBFA_001853"
     CDS             10689..11366
                     /locus_tag="ACHBFA_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM9395329.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         3234 a         2471 c         2040 g         3623 t
ORIGIN      
        1 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
       61 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
      121 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
      181 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
      241 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
      301 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
      361 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
      421 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
      481 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
      541 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
      601 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
      661 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
      721 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
      781 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
      841 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
      901 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
      961 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     1021 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     1081 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     1141 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     1201 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     1261 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     1321 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     1381 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     1441 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     1501 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     1561 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     1621 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     1681 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     1741 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     1801 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     1861 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     1921 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     1981 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     2041 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     2101 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     2161 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     2221 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     2281 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     2341 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     2401 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     2461 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     2521 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     2581 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     2641 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     2701 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     2761 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     2821 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     2881 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     2941 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     3001 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     3061 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     3121 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     3181 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat
     3241 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     3301 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     3361 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     3421 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     3481 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     3541 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     3601 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     3661 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     3721 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     3781 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     3841 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     3901 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     3961 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     4021 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     4081 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     4141 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     4201 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     4261 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     4321 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     4381 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     4441 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     4501 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     4561 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     4621 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     4681 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     4741 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     4801 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     4861 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     4921 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     4981 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     5041 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     5101 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     5161 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
     5221 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
     5281 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
     5341 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
     5401 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
     5461 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
     5521 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
     5581 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
     5641 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
     5701 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
     5761 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
     5821 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
     5881 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
     5941 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
     6001 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
     6061 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
     6121 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
     6181 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
     6241 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
     6301 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
     6361 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
     6421 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
     6481 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
     6541 gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag
     6601 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
     6661 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
     6721 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
     6781 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
     6841 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
     6901 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
     6961 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
     7021 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
     7081 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
     7141 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
     7201 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
     7261 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
     7321 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
     7381 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
     7441 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
     7501 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
     7561 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
     7621 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
     7681 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
     7741 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
     7801 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
     7861 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
     7921 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc
     7981 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
     8041 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
     8101 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
     8161 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
     8221 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
     8281 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
     8341 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
     8401 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
     8461 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
     8521 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
     8581 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
     8641 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
     8701 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
     8761 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
     8821 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
     8881 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
     8941 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
     9001 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
     9061 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
     9121 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
     9181 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
     9241 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
     9301 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
     9361 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
     9421 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
     9481 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
     9541 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
     9601 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
     9661 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
     9721 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
     9781 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
     9841 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
     9901 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
     9961 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    10021 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    10081 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    10141 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    10201 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    10261 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    10321 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat
    10381 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    10441 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    10501 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    10561 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    10621 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    10681 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    10741 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    10801 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    10861 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    10921 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    10981 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    11041 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    11101 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    11161 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    11221 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    11281 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    11341 gccatttaca cttgctttct aattaaaa
//
LOCUS       DAYYNG010000088        11333 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000088 DAYYNG010000000
VERSION     DAYYNG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.088"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..309
                     /locus_tag="ACHBFA_001854"
     CDS             109..309
                     /locus_tag="ACHBFA_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9395330.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            456..3245
                     /locus_tag="ACHBFA_001855"
     CDS             456..3245
                     /locus_tag="ACHBFA_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM9395331.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3292..3819
                     /locus_tag="ACHBFA_001856"
     CDS             3292..3819
                     /locus_tag="ACHBFA_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9395332.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3840..5030
                     /locus_tag="ACHBFA_001857"
     CDS             3840..5030
                     /locus_tag="ACHBFA_001857"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9395333.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5070..6368
                     /gene="asnS"
                     /locus_tag="ACHBFA_001858"
     CDS             5070..6368
                     /gene="asnS"
                     /locus_tag="ACHBFA_001858"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9395334.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7163..7837
                     /locus_tag="ACHBFA_001859"
     CDS             7163..7837
                     /locus_tag="ACHBFA_001859"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9395335.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            7834..8175
                     /locus_tag="ACHBFA_001860"
     CDS             7834..8175
                     /locus_tag="ACHBFA_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395336.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8205..8564
                     /locus_tag="ACHBFA_001861"
     CDS             8205..8564
                     /locus_tag="ACHBFA_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM9395337.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9088..11172
                     /locus_tag="ACHBFA_001862"
     CDS             9088..11172
                     /locus_tag="ACHBFA_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9395338.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         3791 a         1981 c         2459 g         3102 t
ORIGIN      
        1 gtttttgttc ggtaatctgt ggtagcttgt ttcaaataaa catcttccag atatacacca
       61 ttcaagtaat tgcaaaatat gtgttgatac acaaggagga tttttattat ggaaacaggt
      121 acagtaaaat ggtttaactc agaaaaagga tttggattca tcactgcaga aaacggaaac
      181 gatgtattcg ttcatttctc agctatccaa ggcgacggat tcaaaacttt agaagaaggc
      241 caagcagtaa ctttcgatgt tgaagaaggc caacgcggac ctcaagcaac aaacgttact
      301 aaagcttaat taacctatac taaaaaataa acgcccagac atttgcgtgt ctgggcgttt
      361 ttgtctattt tctttaaaaa gcgatgagag tatttttctg cactttgtag tataatttca
      421 aaagaacatt agaggcagga gaagaggaag atattttgaa accaagaact tatgcagtcg
      481 ttgatatcga aacgactggg acgaatccaa aagaagatcg gatcatccaa tttggctgcg
      541 tcatgattga atcaggtcgg atcacttcac gtttttcgat cgatattcat ccagggcgga
      601 agatttcaaa acaaatccag catttaacag ggatcacgaa tcaacgcgtc caaaaagcgc
      661 cttatttcga agatgtcgcc caaacgattt acaatctttt ggcagacacc atatttgtcg
      721 cgcataatat ttattttgat tataattttt tgaaccatga actcatgcga tgcggacttc
      781 ctagtttgaa gataccaggc atcgatacag tagaacttgc acaaattttc ttgccgacgg
      841 aaccaagctt tcgcttagct gatttgtcag aaagccttgg atttattcat gagaatcctc
      901 atcaggcaga tagtgatgcc gaagtgacgg gtcaacttct tttgctgatc gaagaacgga
      961 tgagaaaatt gccaatcatc acgttagagc agattgcgcg attaagtaga cacacaggta
     1021 tggatacgag tcgtttcatt tatcatgtga ttgaagaaat gaaagaaaaa ccagagccac
     1081 ttgatccctc attagaaatc gtcgatgggc tagctttgca gaaaaaagaa gtggaactat
     1141 tcacatccgt ccattatggc gagcggacat atccaagaaa aaaacaagca aaagaaaaaa
     1201 tgtttggtaa aacactgatg tatcgaaaag aacagaacag actgatgaat gcggtctacg
     1261 accatttcac aaaagatgaa tcaaaagatt tgatgatcga agcagctaca ggaatgggta
     1321 aaacaatcgg ttatctgctg ccattgagtt atttggctac acctgaaaaa cccgctgtca
     1381 tcagtaccgt ttcgctagtt ttgcagcagc agatcataga aaaagacatt ccattgctga
     1441 atcaattgct agatcagccc atacaacctg taattatcaa aagctatcgc cactatattg
     1501 atttacagcg ttttaaaggc acacttgtgg aaccgccaga acaaaaacaa tatgctttgt
     1561 atcaaatggc ggtcttagtt tggctgacgc aaacgaagac cggtgatttg gatgaattac
     1621 atttaacaaa tttgaaccat tcattctttg cagatatcgc gcacagaggt attggatttt
     1681 tagcaagaaa tcagtcattt tacgaacaag actttgttcg tttcttgcaa aagaaaatca
     1741 ggcaaagtaa ttttttgatt gtcaatcatg cgtttttaat acaagaaaca cagcgaaaag
     1801 aaccattgct tccagctagt ccatttttgt tgatcgatga agtacatcat ttgccggaaa
     1861 tcgctgaaaa agtaaatgac aaacgattaa gtattaacta tttccataaa caaatccagc
     1921 aattagaaga accaaatcag ctgtttgacc gaatacaaag ctatttaccg aaagaacatg
     1981 aagccttacg ctggttatcg atttatcgag aagaactttc tgcgctatgc gaagcacaga
     2041 tttgtatggc agaaggatta atgagtttag tgagcggcaa gcaacctaac ggacagtatc
     2101 cagaagaagt catgatgaca aaagagatgt tggatcagct gccactggaa tctgggacag
     2161 ccattgatca cctgcagctt ttttatcagg agatccagca gctacagtca caaatgcgtc
     2221 tgacttttga gattatccaa gaaagctgga caaaccagat gcggatcgat ttagcaagtt
     2281 tatatagctt atttgatcaa ttagacaagc agtacttgct gatggatcaa tggctggaag
     2341 actggcgaga tcatttgatc cattggttcg ttcctgggaa cacagcgcag cagatgattt
     2401 ttcatctcca tgattttcaa gcggctatgt tgccgtcgac tgtttggtat cctcgatatg
     2461 atcgtattct ttatattgga ggaacgctga agatcggccc taatcgacac tatctctcta
     2521 agcggcttgg cattgaagca gcacctctta aagtgattgc ctctccttat gattatgaga
     2581 aacaggcaag gatctatgtg ccaaaagagg cacctgccgt ccatcaagtc agcaatcaag
     2641 catatatcga ttatttagag acagcactta caggtctgat cgaacaagaa gatcggccaa
     2701 tacttgtgtt attcacttct catgatgttt tgcagaaggt ctatcagaaa atgcatctgt
     2761 ctatgctaga aaaaggacga gaaatactag ctcaagggtt tggcggaagc cgggagaagc
     2821 ttttgaaacg cttcattttg tcagaaaaaa gcgtattgtt tggcgctgac agcttttggg
     2881 aaggtgtaga tttgcctggg gatgctttgc agatcgttgt agtcacacgt ttacccttcg
     2941 ataatccgct tcgcccgatg gtcaaagcta ggaacgctta tttagaatca gaggggatca
     3001 atccttttta ccaggaatcc gtacctaaag cagctttgaa gttacgccaa gcattaggtc
     3061 gcttgataag gacggagaca gatcgtggcg tattgcttat tttggatcga agactagtca
     3121 ctgcaaaata tggtgcacgg atcatccatg cattaccgaa aaaagtaccg atcaaagagc
     3181 tagctatgga ggaaatctat caggacatcc atgaattctt aaagaaaaat gaagaaggaa
     3241 gttagaggag ctgtcttttt tcataattaa aatctgctat aatgaaaagg aatgaagata
     3301 aaggagcgac gacccttgaa gaaaaaacag acaaaggaaa aaacgattaa tcgcgtgtta
     3361 cttggcttga tcgtattttt attagcggtc atcgtgatca gctcgatttt ttacttgcgt
     3421 tccagtcgtc cggcggtgca ggcgaagaaa gaagcagtag aaatcgcaaa gaaatatgca
     3481 aatcttgagg aagccgatca tttttattgg tttactcgaa agaagacata cttttcactg
     3541 acaggtacaa atgataaagg aaaagaaatc gccgttatca ttcctaaatc tggtgaaaaa
     3601 gtaaaagtat tggatcaaac aaagggactg actgaaaacg aagcaaaaca gacaattgct
     3661 gcggcccatc ctgaaatcca aatcgaaaaa gctgctttag ggatgtatga tgatcagcct
     3721 gtgtgggaag tagtaggaaa agaaacaaat gggaaactga attattacct gattggcttt
     3781 gaatctggag atgaagtcaa gacaatcaag ggaatttaaa aggtaaagga atgaatggga
     3841 tggagttatc aaaaagaaca aaacgattag aaccgtctgt cactttggca gctgcggcaa
     3901 aggccaaggc attaaaagca aaaggcagag atgtgctgag tttgactgtg ggagaaccgg
     3961 attttgcgac accagaaaac atccaagaag cagcaatcga agcaatccgc aatgggaaag
     4021 ctagctatta tacaccaaca gcaggaatcc cagagcttcg acaggcaatc gttgattatc
     4081 tgaagaaaaa ctatggactt gtctatgaac cttcacaaac agtcgtaaca gacggagcga
     4141 aatttgcttt gtataccctt ttccaaacga tccttgaccc gcaagatgaa gtcatcattc
     4201 ctgtgcctta ctgggtcagt tacggtgagc aagtaaaact ggcagaagga cttcctgtct
     4261 ttgtgaaagg ggaagaatcc aatagctgga aagttactgt tgaacagcta gaacaagcac
     4321 gttcagaaaa aacaaaagcc gtaatcatca attctccatc gaatccgact ggaatgattt
     4381 atagcaagaa tgaacttcag gcaatcggtg aatgggcagt aaaaaatgat attttgatag
     4441 ttgctgatga tatttatggt cgtttagttt ataatggcaa tgagtttact ccaattgcaa
     4501 cgatttcaga agcaatcaaa aatcagacca tcatcatcaa tggcgtgtct aaaacatatg
     4561 cgatgacagg ctggcgcatt ggttatgcgg tggggaattc tgagatcatc aatggaatga
     4621 ttgctgttgc ttcccagtca accagtaatc cgacagcagt cagtcaatat gcagctgttg
     4681 aagcattgaa aggtgagcaa gatacagtag aagaaatgcg gaacgccttt gaagaacgcc
     4741 tgaatacgtt atatccgctt gtggcagaac tgccaggtgt ttctcttgaa aagccgcaag
     4801 gagctttcta tctttttcct aatgtaaaag aaacgctacg catgtgtaaa tatgaaaatg
     4861 tcacacaatg ggtagaagat cttttagaag aaacaggggt agcgcttgtg actggcgaag
     4921 gctttggggc accagaaaat gtccgcatga gttatgctac tgatcgcgct acgttagaag
     4981 aagcggtacg cagaatcaag caatttattg agagtaaaag tcaaaattaa tcctgaagaa
     5041 attcaaggaa tataaaagga gagtcatacg tggaacaaat tcaaatcatc gattcgaaaa
     5101 accatgtggg agaaacagta acaatcggcg cttgggtcgc aaataagcga tcaagcggaa
     5161 aaatcacatt tttacaatta agagacggaa cagccttttt ccaaggtgta gttgtaaaaa
     5221 gcgaagtgtc ggaagaagtt ttccaaacag ctaaaaatct acaccaagaa acatccgtgt
     5281 tagtaactgg agaaatccgt gaagacagtc gttcaaaatt tggttatgaa atcggaatta
     5341 caaatgtgga agtggtaagc gagagccacg attatccgat cacaccaaaa gaacacggaa
     5401 cagacttctt gatggatcat cgtcatttat ggctgcgttc ttcaagacaa catgcgatta
     5461 tgcagatcag aaatgaaatc attcgtgcaa cgtatgaatt ctttaatcaa catcattttg
     5521 tcaaaattga tccgccgatc ttgactggtt ctgcaccaga aggcactacg gatctattcg
     5581 aaacaaatta ctttgatcaa aaagcttatt tatctcaaag tggccaatta tacatggaag
     5641 ctgctgctat ggctttcgga aaagtctttt cattcggtcc gactttccgt gctgaaaaat
     5701 caaaaacacg acgtcatttg atcgaattct ggatgatcga accagaaatg gctttcactc
     5761 atcaagaaga aagtttagaa gtacaggaac aatatgtagc attcttagta caaaatgttt
     5821 tagatcattg cgaatacgca ttagatgtac taggacgaga caaagaagtc ttaaaaactt
     5881 atacaaaatt gccattccct cgtattactt acgatgatgc aatcgaatta ctgaagaaaa
     5941 atggatttga tgacatcgaa tggggcgaag actttggttc acctcatgaa acgttcattg
     6001 cgaattcatt cgaacagcct gtctttatcc taaactatcc aaaagccatc aagccattct
     6061 acatgaaacc acatccaaca agagaagacg tggtaatttg tgcagatatg atcgcaccag
     6121 aaggttacgg tgaaattatt ggcggaagcg aacgggcaac ggactatgat ttcttgctag
     6181 aacaaatccg taatcacggt ttggatgaaa aagaatattc atggtatctt gatctacgta
     6241 aatacggttc tgttccgcac tctggttttg gtctaggtct tgaacgaaca gtcacttgga
     6301 ttgctggtat cgaccatgta cgtgaagcta ttccattccc acgtttgttg aaccgaattt
     6361 atccataaga ggttgtgcgc ctgccgttca gctccgagtc acaaggagta atttcaaaaa
     6421 acagtttttc atgtttgatg actattgtga cttgtggccg gagagcaacg tttatttaaa
     6481 aaaaagaact gcgaactctc gcagttcttt tttttaaata cgggataatg gaagtttcca
     6541 gcagacaaga gaagaaagca aaagaaattg ctatttgatt cacaaggttc tataacgaaa
     6601 tcgcaatcaa tcaaacattt acaaagaagg taattcaaat ggaaatctga tttgatgatg
     6661 cttctgctgt tgaaataaag cagcatctag cacctgaaaa caaactaata gtaacattcg
     6721 aagatggagt aggtccgtat tttcagcatg caataatcca tatgtaagtt caattcaccc
     6781 ttgaaatcat cggaccagat aaagttatga ccaatctatc ccaagcaatc tagaagagat
     6841 attagtaaag ggacatttac tagaagtctt agatgagcct ataaatatat gtttcgatgg
     6901 cgtcatcagt ggtctgattg acggaagctt gggtgtgatc gatctctctg gaccaaacaa
     6961 cgaaggaatc aaggggaatc cggcacgtta agaaaaaaat agcataaaaa actagacact
     7021 tgatgagcgt tcattcaatt gtttgaagga actaggctgg tgtcattttt tatacatata
     7081 ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat catcgtttta
     7141 ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg atagcattac
     7201 agacgcagga agataccgag cagaagcaac tgatctaggt aaaggttatc cacttttgat
     7261 tgccggaaaa ctaatctccc aatatccaac gatggaattt gaatgcatga accgtgggat
     7321 aggaggagac aaaattgcag atctgcaagt acgctggcaa gaagattgtt tagctttaca
     7381 acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata ctagcgatca
     7441 ggctatattc ggaactgaga aatctgctca gcaatttgaa tccgtttatc gaaaattact
     7501 aaatgaactt caaacagcgg aaatctgcaa catcatcttg atggagccgt ttgtacttcc
     7561 tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc aaattgttcg
     7621 taaacttgct aaggaatatg gcgcattgct ggttccttta gatggtatat tcaatgcttt
     7681 agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc caacgattgg
     7741 cggacatgag atcattgcac aaagctggat agatgcaatt gtcgcagtcg atttttttag
     7801 tggagataaa aatgagcgaa agaaaagtga aaaatgatgg aaataaggtg ggcagtaaaa
     7861 gaagatttac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt cgtaaaagca
     7921 aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt aacaaaagaa
     7981 aaacggatta tcggatttgc gcaagcggac gtgcaacagt cagaagaacg attggatcag
     8041 cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc
     8101 caaaaaaaat ttgcagaata cttgatgctg gaagctaaaa aaagaagaat aaaatcaatt
     8161 tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt tgtttaggaa
     8221 atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta gcagcgggga
     8281 tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt aagatcgcct
     8341 ccctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc atgtcattga
     8401 tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca catgtcgtca
     8461 gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa tctttcaaac
     8521 ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaagaga ataataaagg
     8581 ttagataaag catagagcca ggtgggatac atgtatttca cctgactcta tgcgttttcc
     8641 tgtataagtt cttctatgtg acttttacgt cttttattta tatggggtta ctgtttatgg
     8701 aaagttgaaa taattttcat taaagaactc tttctatatt tttgaaagcg tagtggagct
     8761 tcgtatatct gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa
     8821 ttaatgaatt tagacaaatt gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag
     8881 atcaagaagg caagtgcggg ttggataaga gcagtggcaa tgatcgaaga gaaaaagaaa
     8941 gaataagcca aaacatctgt atggaagaaa ttttccgatg cagatgtttt tcattttttc
     9001 tttcggatac cttgacatat gaataaccat tcatttataa ttagttatga agagatattc
     9061 atatgttgag gcgaggaggt cattaaaatg aaaagttaca gattagaagg tttagattgt
     9121 gcgaactgtg caatgaaaat cgaaaaagga gttcagaaaa tcaatggagt caaggaagca
     9181 acggtaaact tcacttctgg aaaactaacg attgacgcag aggaagatca tttggctacg
     9241 attgaacaag aaacaaaaaa agtagtgaag gaattagaac cagatgtcaa agtaacagaa
     9301 atagataagg aaaaagtatc tgaacatgga aatgaaaaaa agagaaatac gctgttccgg
     9361 attttatttt cccttgcagg tatcgcttta ttgctattat ttgattttaa tgaaccaatt
     9421 cgcttgatag ggtatttatt gatttatctg ctgataggat atgatgtagt gaagaaggca
     9481 gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt ttcttatgtc tgttgctacg
     9541 attggcgcta tgttgatcgg tgaatatcca gaagccgtag cagtgatgct attttatcaa
     9601 atcggcgagt atttccaagg attggcagtc agtcattcaa gaaaatcgat tcgcgaattg
     9661 atggcgatcc gtccagaaac agctcacgtg cagacggcgg aaggattgat gaccgtgaac
     9721 cctgaagacg tgctgatcgg tcagtttgta cttgtgaagc ctggtgaacg tgtaccgtta
     9781 gatggaacga tcatcgaagg agaatcatta gtcgatacgt cagcacttac aggagaatca
     9841 gtccctaaat ctgtatatgt tggtgaaaca gtattaagcg gatttatcaa taaaaataaa
     9901 ccatttttag ttcaagtaga aaaatcttat gaaaattcta ctatcagcaa attattagaa
     9961 ttagtagaaa atgccagcag taaaaaagca ccagctgaaa atttcattac taaattcgcg
    10021 cgctattaca caccagtcgt tgtaggattg gccgttttat tagctgtttt acctccactt
    10081 gttgtttcta gagcggcgtt ttctgaatgg atctatcgag cattgacatt tttagtgatc
    10141 tcatgtccat gtgcattagt gatttccgtg ccacttagtt tctttggcgg tatcggagga
    10201 gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact tggagttgct tgcacaaact
    10261 gagacagtgg tattcgataa aactggcaca ttaacgaaag gtgacttttc gatccagacg
    10321 atcgatccaa caggaattga tccaaagaaa tttatgcaat atgttgcaag tgcagaacag
    10381 ttttctacac atccgattgc tcaatctgtt ttagaaggct acgcaggtcc gcttttacca
    10441 acagctaata tccaggaatt tgcaggtgaa gggatcttag ctgaagttga tggcaaacaa
    10501 gtattagtag gtaatcacaa attgatggaa agatttgaaa tctcttttcc ttcaagtcaa
    10561 gaaatcggta cattgcttta tctagcgatc gatcaaagtt acagtggtta tcttgttatt
    10621 gcagatacct tgaaagaaga tgccgtagac gcattggttc aattaaaaca ggctggtgtg
    10681 aaaaacacag tcatgttgac gggcgactcg aaaaaaattg ctgatcatat cgggaagcaa
    10741 gtaggcgtgg acaagatata tagcgaattg cttccagaag ataaagtgca gcggttagag
    10801 gaaatcttac aaagtaacaa taaaaagacc gcatttgtcg gagatggaat caacgatgca
    10861 ccggtgctag caagagcaga tgtaggaatc gcaatgggtg gattgggaag cgatgctgca
    10921 attgaagcag cagatgtagt catcatgaat gatcaaccat cgaagattgc agaagcaatc
    10981 catttagcaa agaaaacatt gaaaatcgtc aaacagaaca ttgtctttgc tattgggata
    11041 aaaattttgg tactttcctt aggtgctttt ggttttgcat caatgggaga tgcagtattt
    11101 gcagatgttg gggtgacagt attagctgtt ttgaatgcca tgcgaagctt gcatgtaaaa
    11161 aataaacgtt gatcaatgaa gaggacgaat gaacgtcctc ttttttttat atccttttct
    11221 taatgaaaaa tgtaaagaaa atagatagga ttataaaata actaatgttt ttaattttgc
    11281 agaaaattca aaatagcttg aaattcaata aggtcaatga aataaaagaa aat
//
LOCUS       DAYYNG010000089        11329 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000089 DAYYNG010000000
VERSION     DAYYNG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.089"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..712)
                     /locus_tag="ACHBFA_001863"
     CDS             complement(56..712)
                     /locus_tag="ACHBFA_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9395339.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            905..1183
                     /locus_tag="ACHBFA_001864"
     CDS             905..1183
                     /locus_tag="ACHBFA_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395340.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1202..2410
                     /locus_tag="ACHBFA_001865"
     CDS             1202..2410
                     /locus_tag="ACHBFA_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9395341.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2558..2923)
                     /locus_tag="ACHBFA_001866"
     CDS             complement(2558..2923)
                     /locus_tag="ACHBFA_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM9395342.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3070..3843)
                     /locus_tag="ACHBFA_001867"
     CDS             complement(3070..3843)
                     /locus_tag="ACHBFA_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9395343.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4017..4877)
                     /locus_tag="ACHBFA_001868"
     CDS             complement(4017..4877)
                     /locus_tag="ACHBFA_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9395344.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4877..6295)
                     /locus_tag="ACHBFA_001869"
     CDS             complement(4877..6295)
                     /locus_tag="ACHBFA_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9395345.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6352..6801)
                     /locus_tag="ACHBFA_001870"
     CDS             complement(6352..6801)
                     /locus_tag="ACHBFA_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395346.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7246..7509)
                     /locus_tag="ACHBFA_001871"
     CDS             complement(7246..7509)
                     /locus_tag="ACHBFA_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9395347.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7608..8903)
                     /gene="rho"
                     /locus_tag="ACHBFA_001872"
     CDS             complement(7608..8903)
                     /gene="rho"
                     /locus_tag="ACHBFA_001872"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM9395348.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8900..10171)
                     /locus_tag="ACHBFA_001873"
     CDS             complement(8900..10171)
                     /locus_tag="ACHBFA_001873"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9395349.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10336..11205)
                     /locus_tag="ACHBFA_001874"
     CDS             complement(10336..11205)
                     /locus_tag="ACHBFA_001874"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9395350.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         3177 a         2390 c         1962 g         3800 t
ORIGIN      
        1 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt gttcatcaaa
       61 agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg tctttaacgg
      121 tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta gcagttgaga
      181 tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc atcccttctt
      241 ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca gctcccagct
      301 ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga tagcggtaat
      361 actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa aagcgcacac
      421 gattgattcg ataaaactct gcatattctg attcaactcg aatattatac ggcgaatcga
      481 tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg atatagcgaa
      541 ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc tctttcaaga
      601 tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat cctttgcgta
      661 taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc aatgatgctc
      721 ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt taaagtccaa
      781 aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac ttatgcgatt
      841 tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa tagagggaaa
      901 aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat atcaaacaag
      961 atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt
     1021 gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa atctgtttat
     1081 gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca attatcacga
     1141 tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat gtataacata
     1201 catggaaaag aaattattta ataaatattt tattggaatc acattgatca attttattgt
     1261 ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag acaccttaca
     1321 cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa cattgctggc
     1381 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
     1441 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
     1501 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
     1561 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
     1621 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
     1681 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
     1741 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
     1801 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
     1861 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
     1921 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
     1981 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
     2041 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
     2101 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
     2161 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
     2221 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
     2281 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
     2341 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     2401 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     2461 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     2521 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     2581 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     2641 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     2701 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     2761 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     2821 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     2881 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     2941 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     3001 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     3061 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     3121 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     3181 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     3241 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     3301 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     3361 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     3421 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     3481 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     3541 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     3601 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     3661 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     3721 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     3781 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     3841 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     3901 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     3961 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     4021 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     4081 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     4141 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     4201 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     4261 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     4321 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     4381 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     4441 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     4501 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     4561 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     4621 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     4681 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     4741 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     4801 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     4861 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     4921 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     4981 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     5041 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     5101 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     5161 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     5221 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     5281 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     5341 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     5401 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     5461 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     5521 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     5581 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     5641 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     5701 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     5761 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     5821 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     5881 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     5941 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     6001 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     6061 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     6121 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     6181 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     6241 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     6301 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     6361 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     6421 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     6481 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     6541 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     6601 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     6661 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     6721 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     6781 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     6841 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     6901 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     6961 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     7021 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     7081 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     7141 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     7201 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     7261 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     7321 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     7381 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     7441 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     7501 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     7561 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     7621 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     7681 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     7741 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     7801 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     7861 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     7921 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     7981 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     8041 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     8101 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     8161 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     8221 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     8281 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     8341 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     8401 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     8461 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     8521 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     8581 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     8641 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     8701 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     8761 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     8821 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     8881 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     8941 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     9001 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     9061 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     9121 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     9181 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     9241 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     9301 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     9361 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     9421 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     9481 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     9541 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     9601 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     9661 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     9721 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     9781 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     9841 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     9901 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     9961 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
    10021 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
    10081 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
    10141 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
    10201 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
    10261 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    10321 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    10381 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    10441 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    10501 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    10561 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    10621 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    10681 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    10741 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    10801 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    10861 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    10921 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    10981 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    11041 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    11101 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    11161 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    11221 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    11281 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaaga
//
LOCUS       DAYYNG010000090        11304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000090 DAYYNG010000000
VERSION     DAYYNG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.090"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(335..1453)
                     /locus_tag="ACHBFA_001875"
     CDS             complement(335..1453)
                     /locus_tag="ACHBFA_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395351.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1686..1808
                     /locus_tag="ACHBFA_001876"
     CDS             1686..1808
                     /locus_tag="ACHBFA_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395352.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1924..3231
                     /locus_tag="ACHBFA_001877"
     CDS             1924..3231
                     /locus_tag="ACHBFA_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9395353.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3350..4549
                     /locus_tag="ACHBFA_001878"
     CDS             3350..4549
                     /locus_tag="ACHBFA_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9395354.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4576..5844)
                     /locus_tag="ACHBFA_001879"
     CDS             complement(4576..5844)
                     /locus_tag="ACHBFA_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM9395355.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5899..5965)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6007..6653)
                     /locus_tag="ACHBFA_001880"
                     /pseudo
     CDS             complement(6007..6653)
                     /locus_tag="ACHBFA_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7061..7564)
                     /locus_tag="ACHBFA_001881"
     CDS             complement(7061..7564)
                     /locus_tag="ACHBFA_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9395356.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7687..8451)
                     /locus_tag="ACHBFA_001882"
     CDS             complement(7687..8451)
                     /locus_tag="ACHBFA_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9395357.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8559..8834
                     /locus_tag="ACHBFA_001883"
     CDS             8559..8834
                     /locus_tag="ACHBFA_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9395358.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9272..10222
                     /gene="yidC"
                     /locus_tag="ACHBFA_001884"
     CDS             9272..10222
                     /gene="yidC"
                     /locus_tag="ACHBFA_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9395359.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10401..10580)
                     /locus_tag="ACHBFA_001885"
                     /pseudo
     CDS             complement(10401..10580)
                     /locus_tag="ACHBFA_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3391 a         2381 c         1995 g         3537 t
ORIGIN      
        1 gaaataagcc gaaatttcac aaaaatttga gaaataattt ttgtcaatct cttcttattt
       61 cttgaagctg accacttctg tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
      121 tgcggtaata agcccaccaa acaaaaaata tgaagagcta tttttgcttg ctgttcttat
      181 tacttgatgc agaacagctt tttcacaacc tctcatacac ggtgtttaat cagctgacct
      241 tcggtattag ctcaaaaaac ggaaaaatat gtgaaactat ttttccgttt gtttctctaa
      301 tactcaggtc aaaacactga tttcacaacc tcttttaata acgaagaatc gctgtcagac
      361 tcttttcatc tggcgcatct ttttgatcca aaataaacac tttcccgcca ttaatgatca
      421 catcatctgc taatgcattc agcacttgac gccgatcata ttctgtttca ggatcttcac
      481 cgaaaccatc agctaagttt gacgtagcga taaataaatg tgacactttt ccttctttcg
      541 cagctggaac gatatcaact aactgatcga tgaatttctt atctaagagt ttattgtatt
      601 cttttgattc ttgagctgcg agttcacttg acagcttctt cgccccttct tcgatatctc
      661 taaaggatag ctgcgcagga gaagcagaaa tcgcaacgtc tgcacaataa tagggatttt
      721 tagcaatttt tttgaacatc gtctgatttt ctggtaatgc aaataaataa atcggccatt
      781 gttcaggatt ttcgaaagaa tccttcaagt acgtatcaac agcttggtaa tagttgaccc
      841 aatcgatctc tacttcttca tcttttggat ttacgccatg gaaagttact ccatctcggg
      901 ctccgcctcc tccattagaa gagaagttca aaccgccacc tgtcagttca gatcccaatg
      961 cagtaacgac atcaaccggt gccccttctg gtagagatac ttcttttact tgtttgttgc
     1021 tgacctcata caatttcatg gagtcgcggt tcaatcccaa taaataataa gaatagttga
     1081 actgcccatt tttgataata gccagtaaat aaggaagatc acttacatag tattgatcgt
     1141 ctaccgggat actcaaacga tggatatagg tctggtcagc agttataatg accgctacac
     1201 tcgtagttgc actacgccaa aacgaaggat ctcctaacaa attttcaatt ttttcttgga
     1261 acggggacca gtcatgttcg gaatattttt tctcaaaacg tttctttgct tctttcgcaa
     1321 aatttttgaa tttgatctga tctttttcca catcttgatg ggcgacatgc gtattcagca
     1381 tgatcgtgac gaatggtcct ttagcgcctt ctgaagtcaa ttgtgtcaat aattctttat
     1441 ccttttttgc cataaacgta cccctcctaa aaaaatcatt cctttgtttc tatctaaagt
     1501 ctaccacaac actatttttc catacaataa aacgtatcta aaaaaataaa acggtttcta
     1561 cggttttaaa tttagctatt tttttaagtt atcttcataa tttcctcaat tatttagccc
     1621 cagtcttcaa ctattcttca tgaaatgctg ataaactaag aaaaagaata aaagaggtgg
     1681 atcttatgca tttgattatg attttcagtt tattaagtat tatcagttta tttgctttca
     1741 gcgcttcccg cttattagat gcggaaacac agcctattcc tgtaaaagta cgtaaaaacg
     1801 accgctaaac ttccataacg cgcgttctta tcctaaagtt ttttcagaaa cattgaaact
     1861 ctctcccctc tctgataaaa ttttaatttg tagcgaaaca aattaattac aaggagatcg
     1921 gatatgtcca taaaaaaaac acgtctagtc ctattattgg ctagtctcat tctttgcctt
     1981 gcttcttttc taccagctgt tgctgtgcaa gctgaagata cgttcaaagt aaatgcaaaa
     2041 gcagcttttg ctgtagatgc gcaaagcggt aagattcttt atgatcaaga tggagaaaaa
     2101 accatgggga tcgcttcaat cacaaaaatc atcggtcttt acatcgtcct tgatcaagta
     2161 aaagaaggaa aactgtcatg gggtgataaa gtttcgattt cagattatgc agaaaatttg
     2221 agtatcaccc cagatttatc gaatgttcct ttgcataaag aaaacaccta cactgtcaaa
     2281 gaattattcg actctgcaat catccagtca gccaatgctt caatggtggc cttggcagaa
     2341 aaaatctctg gcagcgaagc taaattcact gaacgaatga aagaacaatt gaaagattgg
     2401 ggaatcaaag atgcaacaat cgttaatgca tcaggattga acaactctta tcttggcgaa
     2461 aaccgaccag aaggcactgg agaaaacgac gaaaatcaaa tgtctgctca agatgtggcc
     2521 atcgttgccc gccatttgat cttggatttc ccagaaatac tggatgtatc aagtactacg
     2581 acccaaatgt ttggcgaaaa tacgcaatca ccagtggaaa tggtcaactg gaactggatg
     2641 ctcccaggat tcgttaatta caaggaaggc gtggacggat taaaaactgg tacgactgag
     2701 cttgcaggtg cttgtttcgt tggtacgatc actaaaaacg atcaacggat catcactgtc
     2761 gtattgaatg cggacggcca tgcggaaaac ccgagtgccc gcttcaatga aacagaccgt
     2821 ttgatggact actgctatga caattggtca gaaaaagagc tcggaaaagc taacgcaagt
     2881 attcctagtc taaaaaccat tgatgtaaaa gacggaaaag aatcaacagt tcctgttgtg
     2941 cttaaaagcc ctgtaaaagt atgggtacga aacgatatgg atactggcaa attaacgatc
     3001 acgccaacac ttgacaagaa gcaagtcact gacaatgaga tccaagcacc tatggataaa
     3061 ggtacaaaaa tcggtaccgc tacgatcacc ttggcagacg ataaattagg ctatttagaa
     3121 gacaacaatg caccatcaac agagatcatt acggataaaa cagtggaaaa agcgaatata
     3181 ttcgtgattg gctggcgacg cgtcgcagaa tttttcggca acttgtttta attgaacaat
     3241 aagaaaaaca tctttccatc ggaaagatgt ttttttgctt tttctagttt aaataacagc
     3301 ttttttcatc tattttcgtt atactgtata agaaaaagga gttgaattta tgaatctaac
     3361 cagagatctg atctttttag ttctctatct tattggggta ttctctatcg tttattggcg
     3421 aaaacagaag aaggatcctt ccttgttctt ttacggcagt acgtggacat ttacggtaat
     3481 cttaacttat tttgtcgtgt tggaaatttt ttattcgacc ttttattttt tgattccatt
     3541 gttgttcttt agtatctttg ctttttctta tttcacagaa aaacgaaggc tactgaatgg
     3601 tcttttattc aatgttttac tcatcagttt cggcatctac ttgtttgtct tgctgtatga
     3661 aacgcaaaat atttttcttg gcggactgat cgccttgatc acgattccat tgctacttgt
     3721 ccttttattt ggtatttatg gattgattgt ttttcttttt tggaacgggg tcacagtttt
     3781 acgtagagaa tcgcattcat tagctaatct tttaacgttg atactagcca tttttcttac
     3841 actgtttctt gtttttgact ttttcttgtt aaaatatttg ccgcaatgga taaatgcttt
     3901 gtttttctgc gttcccctga tcttgatcta tctatttatc gtattctaca actttttgac
     3961 tgtctcattc ttgtatcagt tcaaccggcc tcgatacaat caagacttca tcgttgtgtt
     4021 aggcgctgga ttgatcaatg gagaaacagt ttctcctctt ctagccaagc gaatcaataa
     4081 ggcaattgct ttttatcggg ctcaaagccg tgctacatta aatccgccca tcttattgat
     4141 gtctggcggg caaggagcag acgaaaaagt accagaggcc attgcgatga aacaatatgc
     4201 catggaacaa ggaatccctg aaagagacat tcttgtcgaa accaattcga caaccacttt
     4261 ggaaaacatg ctctattcaa aggaaatcat ggatcagcaa atgaaagggc cataccgcgc
     4321 gatctttagt tccaacaatt accacatttt tagagccggt ctttatgcgc ggcaagccaa
     4381 attgaaagca aatggtattg gtgcaaaaac agctttctac tacttgccta atgcgttttt
     4441 aagagaatac ctcgccatct tgatgatgaa caaaaaaaga catatgatca tttgcggatt
     4501 gcttttcgca ggctccgcat tcttatcgct gattacttta cttttctaaa aaaacaaggt
     4561 gtgacaaaac atatgtcaca ccttgttttt ttctttcatt ctcttaacta ttggcaaaaa
     4621 gagtatccct tttttgaccg ctacttgctg agcagaataa ataccgctgt tgcctgaaca
     4681 cataaagctg atcaagcaga tcataaagaa gtatactgcc gcttgtgaac cgaacaattc
     4741 gatccccatc acaaagcagg caatcggtgt atttgtcgca ccagaaaata ctccgataaa
     4801 gcccaatcct gcaagaaaag gaacagaaac atggagcaaa ggcgctagtg ctgcaccaag
     4861 cgttgcacca atttcaaaca atggtgtcac ttctccccct tgatatcccg cgcctaaaga
     4921 caaaactgtg aagaataact tcccaataaa atcatagaca tgtgcctgtc cattaaatga
     4981 ttcttgaagt aacggaagac tcaatccgag ataacgttgt gtttgcaaaa tcaatgctgc
     5041 tgccacaact acagctccac ctatcgtatt tcgacaaaca ggatgaggaa tccattgtgc
     5101 atatcgcgct tttaaaaaga cgatcgaacg gctgaatacc caaccaatca aaccaaaagc
     5161 aatcccagca acaaaaactt tgaagaacag caacatactc cattcaggaa tcacacccat
     5221 ttggtaatgt agatgagaaa caccatagct ttctgtcatg aaatttgcaa acagtgcagc
     5281 aaagaagcta gggaagatcg cttctgttcg gactttcccg attgccaaca cttctaatcc
     5341 aaataatgtt ccagctaaag gtgtgccaaa tacagaacta aaacccgcac tgattccact
     5401 gataatcaag atttcccgtt ctgatttatc caatcggaac aaacgtgccg tattatctgc
     5461 taacgctccc cccatttgga ctgctgttcc ttcacggcca acagatcccc caaataaatg
     5521 ggtagttatg gttccaaaca gtgtaagtgg aataagccgc aacgggattt tttcttctcc
     5581 accatttcct tgatcgatca ctaagttgtt tccacgacta gaaagtccgc cgtgatacgc
     5641 atagagataa gcaaaaagcg caccactaac tggtagtaga caaaggagcc aaggatgtga
     5701 tagtcggata tctgtcacaa ctgtcaggct atttaagaaa aaagcagaca agctccccat
     5761 gaatataccg agaatcgctg tgatcaccag ccacttcagc atatacacta aaatgcgtac
     5821 tggactcggt aattgttttt ccataaaaaa ttcctcctca ataaaaaaac gcctactaaa
     5881 gaaaggggct gtccccagac tttagtaggc gtcattagct atctttcgtt agcatttaaa
     5941 ggcgaacacc atcacctatt cacttccatt aaaagatagc atatttttct gtaaaaaaca
     6001 ataaaattat cgttctttct ttacacgttc agcaaattca tagaaaggag ccaatttcgt
     6061 taactgttga ttacattggt cactatcatg acaaatataa ttcccctttt tcgtatacgt
     6121 gccatcagaa cctgctttcg ttgtagaaag aaaaagcgaa acattcgatg ttttctgaca
     6181 gattgcacag attcccttat ttatcgttgg tgataaagta ccgctaatcc cttttaattt
     6241 ttcatcttcg taatagacca tgaatttttt ctgcgtacct ggatcattcc agccgatgta
     6301 cgtatgttct tgcaagtcga tttcattcca agctggttgt ttgagttttt tcacttttcg
     6361 gaataatttc tcgatttgtg gtttgctagg ctgcataaac ggctgaacga tttgcttcag
     6421 ctcctccaat gctctttctg cttgcgcttt tgtcagtttt acgtctaaaa ctgtcgttaa
     6481 aaatgtatcg attctggcag ttcgtgatct aatatgtctc gaatcttttc agctgctagt
     6541 gcctgaactg tttgtaccgt ttttggatca ttgacagatg agtacgcatt taataactgt
     6601 tctacttctc ttattataaa aaaatattgg tatggtttta ttgtttgttc catgatttta
     6661 aagtcctttc ttctaaccaa agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta
     6721 tgtgttacct gaagatcccg gactctttta aataagatac gttttgcatc ttatcatcac
     6781 cccctttaat gcttattatt ctatgtacat gattcattcg ataaataaaa gtaaaccgta
     6841 tcttgaacag atagtcaact gcaaacagaa aaaaggactg tgacaaaagt cgtgtcaaag
     6901 tcccttgttt cgaatgaatg gtgttcaaaa gctggcatct gcggtaataa gccgaaactt
     6961 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
     7021 ctgtcacaac ctcaatatgg tctattattg gttttatcta ttaaatcgag gattctttcc
     7081 caaagtaacg ttgccatctc ttacgcatac ttgctgacat aggctgagcg gcttctttga
     7141 ctgcgcagta tttgtctacg aatgttacga tatatccttc tttgtactta ggcggtgcaa
     7201 tcgttgcgcc ccacatgtgc ttcaaaatga tatctcgctc aagatccgac aattcggtga
     7261 tcttttctgc attctttaca gcaattcttg gatggatata cgcatgtgtg ccttcatcaa
     7321 atttcgtcgt acgccaatca tagtagaaca gatcatgcaa aagtccagca cgcgctgttg
     7381 ctttagcatt gccaccccat ttttttgcga gcagataact atagtaagaa acactgatgg
     7441 aatgctccaa acgagtggaa tgaacatgct gtgtgtaatt cgctaatttt ttcactgctt
     7501 ctgtttctaa aagatcttct acataagaca tatattcttc gtcttcacgc catttttctg
     7561 tcataattat cgatctgcca cctttttgaa tttcattttc ttctgaaatt gatctaagta
     7621 taccaccagt gttcggatta ttctagtcgc cttggataaa atagccaaag cttaatagtt
     7681 ccttaatcat aaagaaaaat gataaagaac aatccctgcc gcaaccccaa cattcaacga
     7741 ttccgcattt cctttcatcg ggatatatac gttctgatca gtcaaagaca agatttcctg
     7801 acttaccccc tgaccttcgt tccccatgat tattgcgaag ttatctgatt tttctacttt
     7861 gtctaatgcg tgtgcttctt catttaattc tgtgccatac actgtaaacc ctttgtcttt
     7921 cattaaagaa atcacttctg tcaaatcttt ttgcaacaca ggcaaatgat aattgcttcc
     7981 ctgcatgctg cgcagcacct ttgtactata aatatctgcc gttcctttgc ctaataaaac
     8041 accagccata tcaaatgcat ctgccgtacg gatcatcgtc cctacatttc caggatcttg
     8101 tacattatct aacaacagcc atcctcctga aaaatctggc gtgccttcag ccgttaacgc
     8161 catcactgct aagattcctt gtggtgtagg tagatccgat acagcatcca tgacctcatc
     8221 cgaaactaga aaagtagaaa tctctttgtt tgcggccagc cagtctcccc attctttcat
     8281 gcctcgctgt gtcacaaaaa gttcttttat ttctgcatca gacgacgcag cttcttcaat
     8341 cagatggaac ccttccaaaa gatattcttc cttttcttcc cgatatttct ttttatgtag
     8401 tttttttaat tctttgattc ttggattttt tgctgattgg atctctttca cattcctcac
     8461 ctccccatga ttatatcatg gaataggtgc ttgagcttgt ttcatcctaa aaaaatcggt
     8521 atcattaatt tataaaatag gaaagaaagg tgacgaacat gcgcaaagtt aaaatgaacg
     8581 tccaaggacg cgtccaaggc gtaggatttc gctacatgac caagatggtc gctgatcaac
     8641 taggcgtgac tggaacggta aaaaacgaag atgacggctc agtcacgatc gaagcaatcg
     8701 gcaatgacga catcatccag aaatttattg aagaagtcaa gaaatctccg agcccaagtg
     8761 gacgagtgca gtatgtggat gtccaagaag acccgatgat tgaggaacga aagaaatttg
     8821 atgtggtggg ataaggttgt gaaaaagctg gtctgcatca agcattaagg acaggttgtg
     8881 aaaaatagct ctccatattt ttcaacaggc tggacttaat gccgtagatg caagcttttg
     8941 aacaccgtcg ataaggttat tcttgaggcg ctttgcctca aaaattaagg acagcagtga
     9001 aaaagctgat ctatcacttg cagtgagttc atttgactcc gctttttcgg gcagaaaaaa
     9061 agtttttagt ttattaaagt aagaaagaaa cacttggaat cattctactt tttcctagta
     9121 tttctttctt gattttttgt ctttttttca aaatttttcg ctacgtaaaa gaacgaatat
     9181 ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta tagttaagtt
     9241 tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact ggttattggg
     9301 atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg attcaaacgg
     9361 aaaccctgac acttcgggaa tcgtttatcg agtactcgtc catcctatgg gccaagcgat
     9421 tacatatcta gttgaaaatt tcaattggtc ttatggctgg gcagttattg caatgactgt
     9481 gatcgtccgt atcatcatct tgccactagg tattagtcaa tcgaaaaaaa caatgatcca
     9541 atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcccaacaaa aattgaaagc
     9601 tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag tataccgcga
     9661 aaacggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc aaatgccgat
     9721 cttctctgcc ttgtacttta cggcacgtta tacagaaggt atccgggaat catctttcta
     9781 cggcatcgat ttaggctcac caagtatggt acttgttgcc attgcagggg tcgcttatct
     9841 gcttcaagga tacatctcaa cgatcggtat tcctgaagaa cagaaaaaga cgatgcgtac
     9901 gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc cagccggtgt
     9961 tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca tcacaaacgt
    10021 tatgatgaaa ccacgtttga aagcacaagt ggcagaagag ttgaaacaaa acccaccaaa
    10081 acaagtcgtc actccaagaa ttgatgtgac agaatcagct aaaccagctg acagcaaaca
    10141 agcaaccaaa caaatcaagc aacccaaaaa cagtacaaac aataaaggcc gcaacgcagg
    10201 aaagcagcag aagcggaagt agatagtaaa taagagtgta ataaaaattc attaacagtt
    10261 ttagcaatta aaaatatcga ccaagatttt tgaaaaagaa atctggtcga tatttttatt
    10321 taagtgaatg tttaatttga atcatcgatt gatcgctatc taattgcagc agcatctttt
    10381 tatgaagaaa caactttttc ttatctatca acgtagacag gtgttgctgt ttcttctaat
    10441 agtgtactta attccttaat attttgtttt ccttcttcta acatccaact actcgctttt
    10501 tcttcgcctt cggtaacaaa aggcgccact cccttcgccc atgttgctcg cccacataag
    10561 acaccattct ccataaagga caacgcggag ataccaccca tctaaataac atccataaac
    10621 ctttctagta gaagaatagt gatagtatcc atcccttttt ctactgggaa tacaattaca
    10681 cgattcaaag cttgatacac aacatttccc ttcttataaa ccgctattgt aaccgaattg
    10741 ttttgaatca cctttttggg atagaaactg gctcctttcc gcaacttctt aaatgttggt
    10801 ttcttttata ctttctttgt agcaactaca aatttcttca aatctctaaa tgtttgcata
    10861 acaaccgcct ttctgacttt tactcaggat ggtatttaaa aatcagcaaa ataaggcttc
    10921 tctccatctt ttttatttcc tagctgcttt aaagacactc ttgctgtttt ttaatctcta
    10981 tcaagggggc tctgaatcta ttttgatgaa cttatcttat taatttctca aatgagctgt
    11041 cagacacaac tgtatttttt caatctcata ccggacaact atgccagctt aaaaaaattt
    11101 cctctggcaa aacttcttta ttgaaaaatg acaatgaaaa aagaccattc tttttgttga
    11161 atgctcattg tgtaaacata tttcctgcga ctgctcttcg aaccattctc agtgcaatcc
    11221 ctaatttatc tattaaatct tcttttagtt cctcatcttt catattcaaa atttcctttg
    11281 tatgttacac aagtttgttc tcta
//
LOCUS       DAYYNG010000091        11160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000091 DAYYNG010000000
VERSION     DAYYNG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.091"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHBFA_001886"
     CDS             6..305
                     /locus_tag="ACHBFA_001886"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395360.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHBFA_001887"
     CDS             378..1739
                     /locus_tag="ACHBFA_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9395361.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHBFA_001888"
     CDS             1761..2021
                     /locus_tag="ACHBFA_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395362.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHBFA_001889"
     CDS             2035..2787
                     /locus_tag="ACHBFA_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM9395363.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHBFA_001890"
     CDS             complement(2881..3486)
                     /locus_tag="ACHBFA_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9395364.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHBFA_001891"
     CDS             complement(3539..4513)
                     /locus_tag="ACHBFA_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM9395365.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHBFA_001892"
     CDS             complement(4891..5610)
                     /locus_tag="ACHBFA_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395366.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBFA_001893"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBFA_001893"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9395367.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHBFA_001894"
     CDS             7161..8558
                     /locus_tag="ACHBFA_001894"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9395368.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHBFA_001895"
     CDS             8639..9079
                     /locus_tag="ACHBFA_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9395369.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHBFA_001896"
     CDS             9227..9553
                     /locus_tag="ACHBFA_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9395370.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHBFA_001897"
     CDS             9554..10303
                     /locus_tag="ACHBFA_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM9395371.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHBFA_001898"
     CDS             complement(10383..10838)
                     /locus_tag="ACHBFA_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9395372.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3544 a         1955 c         2293 g         3368 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
//
LOCUS       DAYYNG010000092        10879 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000092 DAYYNG010000000
VERSION     DAYYNG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.092"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHBFA_001899"
     CDS             476..1339
                     /locus_tag="ACHBFA_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9395373.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHBFA_001900"
     CDS             complement(1468..3090)
                     /locus_tag="ACHBFA_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9395374.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHBFA_001901"
     CDS             3339..3860
                     /locus_tag="ACHBFA_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9395375.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBFA_001902"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHBFA_001902"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9395376.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHBFA_001903"
     CDS             5941..6759
                     /locus_tag="ACHBFA_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM9395377.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHBFA_001904"
     CDS             complement(6862..7059)
                     /locus_tag="ACHBFA_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395378.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHBFA_001905"
     CDS             7228..7806
                     /locus_tag="ACHBFA_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9395379.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHBFA_001906"
     CDS             7901..8038
                     /locus_tag="ACHBFA_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395380.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACHBFA_001907"
     CDS             complement(8851..9354)
                     /locus_tag="ACHBFA_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9395381.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACHBFA_001908"
     CDS             9713..10738
                     /locus_tag="ACHBFA_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9395382.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         3333 a         1890 c         2115 g         3541 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaga aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttata
//
LOCUS       DAYYNG010000093        10820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000093 DAYYNG010000000
VERSION     DAYYNG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.093"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..424)
                     /locus_tag="ACHBFA_001909"
     CDS             complement(62..424)
                     /locus_tag="ACHBFA_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9395383.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(435..1556)
                     /gene="alr"
                     /locus_tag="ACHBFA_001910"
     CDS             complement(435..1556)
                     /gene="alr"
                     /locus_tag="ACHBFA_001910"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9395384.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(1572..1922)
                     /gene="acpS"
                     /locus_tag="ACHBFA_001911"
     CDS             complement(1572..1922)
                     /gene="acpS"
                     /locus_tag="ACHBFA_001911"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9395385.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(2052..3563)
                     /locus_tag="ACHBFA_001912"
     CDS             complement(2052..3563)
                     /locus_tag="ACHBFA_001912"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9395386.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(3885..5237)
                     /gene="murF"
                     /locus_tag="ACHBFA_001913"
     CDS             complement(3885..5237)
                     /gene="murF"
                     /locus_tag="ACHBFA_001913"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9395387.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(5390..6466)
                     /locus_tag="ACHBFA_001914"
     CDS             complement(5390..6466)
                     /locus_tag="ACHBFA_001914"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9395388.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            6662..8407
                     /locus_tag="ACHBFA_001915"
     CDS             6662..8407
                     /locus_tag="ACHBFA_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM9395389.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(8687..9124)
                     /locus_tag="ACHBFA_001916"
     CDS             complement(8687..9124)
                     /locus_tag="ACHBFA_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM9395390.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(9108..10481)
                     /locus_tag="ACHBFA_001917"
     CDS             complement(9108..10481)
                     /locus_tag="ACHBFA_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395391.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
BASE COUNT         3067 a         2341 c         1997 g         3415 t
ORIGIN      
        1 tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta cttcgctata
       61 tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat gctcggtcaa
      121 cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt gatttatcga
      181 tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt ccagtagctt
      241 tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg gccgcaacaa
      301 tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact gggcgtgttc
      361 cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct cctcttttta
      421 ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa tccgcaagcc
      481 acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat ttctttgtct
      541 gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg caatcggatc
      601 atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc taccaataga
      661 gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat cgttccgatc
      721 cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc tgctgggagt
      781 tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg ataaacttcc
      841 ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg aatcatattg
      901 ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac atatcgagga
      961 agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt ccaatatgta
     1021 tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc taatacacga
     1081 ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa tccgatacga
     1141 cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt acttttctct
     1201 aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga taaatcatga
     1261 gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat gggctgagta
     1321 aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa gcccgaagct
     1381 ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc gttcgctttt
     1441 actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc tacattttca
     1501 gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac aaccatcgtt
     1561 tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt tgtatgagta
     1621 atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg accattttca
     1681 ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc ccaagcttta
     1741 gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc
     1801 aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt
     1861 tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat tccttttatc
     1921 atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat tataaccatt
     1981 ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa agagaaactt
     2041 tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt atcattgtga
     2101 cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt tgatttgttt
     2161 ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg tttgccattt
     2221 ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt cactggcact
     2281 gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa
     2341 tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt ttctgcaagt
     2401 tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc tttttcggtt
     2461 ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg caagtagtcc
     2521 atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg gcctgtacgt
     2581 ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta cacatgagtg
     2641 acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat atctaaatga
     2701 ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc gccatggatt
     2761 ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac acggcgtttt
     2821 gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg agtcatgata
     2881 tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc agctgtcatt
     2941 tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc aatattcttg
     3001 atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa
     3061 atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc ttcatccaaa
     3121 actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc aagcatacgt
     3181 cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg acggccaata
     3241 tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa acgatataat
     3301 tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc ttgtaattcg
     3361 tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt tttccctgta
     3421 cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat cgtttctgct
     3481 tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa ttctggtgat
     3541 aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact gaggatgtag
     3601 cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt tgacagtggc
     3661 cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca ctttgcgaag
     3721 tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta tgacatctgg
     3781 cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt tcggcaatag
     3841 gttattctaa catagttttt cgttttctgc aagtttacag atttttattt catttgctga
     3901 agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat aatactatct
     3961 gttgctgcaa gattttgttt gatagcatca atcaatttat tcttttcttt ttcaaaatag
     4021 aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg catttgttcc
     4081 ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat ttgcgcatgc
     4141 atatttaaag actctttccc taattccagc atatctgcta acacagcgat tctgcgtcct
     4201 tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg atttgcattg
     4261 tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt tctgttttga
     4321 gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc aaagaagcgc
     4381 ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag cgggatcgta
     4441 aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt cacttccgca
     4501 tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt tgccgttaaa
     4561 ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac aagtccgtca
     4621 gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc tatgtgtgct
     4681 tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag ttccgtgatt
     4741 tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg gggcatgtgc
     4801 aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt atatgttttg
     4861 tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc attgcttcct
     4921 gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa ttgctgcatg
     4981 gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg ttcaacaggc
     5041 aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa ttcatgtcca
     5101 tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg gctgtcaaat
     5161 tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg tgcaatttct
     5221 tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct
     5281 aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt atttagaagc
     5341 tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt tattcatttt
     5401 tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc agttcttcaa
     5461 tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata ctgaactccg
     5521 taaatcctgg catagagttt aattcattca ggaataattc atttttattt gtcaaaaaga
     5581 aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc gcgtactctt
     5641 gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg attttattat
     5701 tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca ccaggcaaag
     5761 tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct tcaattcctt
     5821 gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt tgcagctctt
     5881 ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt ttgacaaaca
     5941 tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt tctttccatt
     6001 gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt aaaatatact
     6061 tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg acataaggca
     6121 tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa
     6181 ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact tttcctgtaa
     6241 agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct ttgctggcag
     6301 gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata aatacgagtt
     6361 gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa agaatggaca
     6421 cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct
     6481 gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa aataaacgaa
     6541 taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt tattttttgt
     6601 cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag gagggatgat
     6661 gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt tcatcaatta
     6721 caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga
     6781 agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag aagatttgct
     6841 ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta gcgacacatt
     6901 ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag atatattcga
     6961 tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc gtgccaagct
     7021 gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg aagagatccg
     7081 acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc ctgaaactag
     7141 cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca atgaaatgga
     7201 cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg gctggaaaga
     7261 gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga
     7321 aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg aaacttttat
     7381 cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag ttgagaactt
     7441 tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag ggaatctgct
     7501 attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg aaacagtcca
     7561 tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag accctgttgc
     7621 gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg caggacttag
     7681 gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa
     7741 agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga
     7801 actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca acttgcaaga
     7861 agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg atcgtgatat
     7921 cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc tcgatcctga
     7981 agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca ctcatctgga
     8041 tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga aggctgcgcc
     8101 taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac aaggaaaagt
     8161 caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta tgatcaatcg
     8221 gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc
     8281 agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt atgctattgc
     8341 cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata agaagaggaa
     8401 aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag gggttttaca
     8461 taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg ctacttctga
     8521 aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag aacaatcaaa
     8581 aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt attcgagcat
     8641 aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag tcaattgcct
     8701 tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt ggaccctttg
     8761 tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga agcgtgccga
     8821 tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc ttcggcaata
     8881 caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta accataccga
     8941 cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg acatgattag
     9001 cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca agaccatagg
     9061 attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca atgaatagcc
     9121 tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct cagtaaaaga
     9181 ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg cactgattca
     9241 tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc taaaacaaac
     9301 aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt agtatacttg
     9361 acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc cttatttagc
     9421 cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc aaaaccattt
     9481 ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc caatggagac
     9541 catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa gcctgctgca
     9601 taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc aacataaaaa
     9661 ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa cagatttagc
     9721 cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa caagatgaac
     9781 agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa caatcgggca
     9841 tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc tcgcgtagct
     9901 accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg actaaccgta
     9961 tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt
    10021 gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa taagtacttt
    10081 ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt ggtattagca
    10141 ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa taaaggaaaa
    10201 taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag agcgatcatc
    10261 agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag tccagtcatc
    10321 cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga gtctgttagt
    10381 cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa gggcgttgtt
    10441 tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca
    10501 gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac caattttaca
    10561 gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct gcatttttac
    10621 ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc gacacccctg
    10681 catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat caattaggag
    10741 ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca cagttcctat
    10801 gtttatgcga gagctttttt
//
LOCUS       DAYYNG010000094        10744 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000094 DAYYNG010000000
VERSION     DAYYNG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.094"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACHBFA_001918"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACHBFA_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9395392.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACHBFA_001919"
     CDS             1995..4661
                     /locus_tag="ACHBFA_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9395393.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACHBFA_001920"
     CDS             5025..5777
                     /locus_tag="ACHBFA_001920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9395394.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACHBFA_001921"
     CDS             complement(5799..6953)
                     /locus_tag="ACHBFA_001921"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9395395.1"
                     /translation="MKIGIDCLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACHBFA_001922"
     CDS             7138..9579
                     /locus_tag="ACHBFA_001922"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9395396.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="ACHBFA_001923"
     CDS             complement(9909..10613)
                     /locus_tag="ACHBFA_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395397.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3425 a         1931 c         2264 g         3124 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacaatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaa
//
LOCUS       DAYYNG010000095        10690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000095 DAYYNG010000000
VERSION     DAYYNG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.095"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..711)
                     /gene="trmB"
                     /locus_tag="ACHBFA_001924"
     CDS             complement(49..711)
                     /gene="trmB"
                     /locus_tag="ACHBFA_001924"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9395398.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(781..1563)
                     /locus_tag="ACHBFA_001925"
     CDS             complement(781..1563)
                     /locus_tag="ACHBFA_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM9395399.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(1767..2978)
                     /locus_tag="ACHBFA_001926"
     CDS             complement(1767..2978)
                     /locus_tag="ACHBFA_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395400.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(2971..3708)
                     /locus_tag="ACHBFA_001927"
     CDS             complement(2971..3708)
                     /locus_tag="ACHBFA_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395401.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            3846..4280
                     /locus_tag="ACHBFA_001928"
     CDS             3846..4280
                     /locus_tag="ACHBFA_001928"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9395402.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            4273..4617
                     /locus_tag="ACHBFA_001929"
     CDS             4273..4617
                     /locus_tag="ACHBFA_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9395403.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            4797..5807
                     /locus_tag="ACHBFA_001930"
     CDS             4797..5807
                     /locus_tag="ACHBFA_001930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9395404.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(5876..6820)
                     /locus_tag="ACHBFA_001931"
     CDS             complement(5876..6820)
                     /locus_tag="ACHBFA_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9395405.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6804..9524)
                     /locus_tag="ACHBFA_001932"
     CDS             complement(6804..9524)
                     /locus_tag="ACHBFA_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9395406.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(9521..>10690)
                     /locus_tag="ACHBFA_001933"
     CDS             complement(9521..>10690)
                     /locus_tag="ACHBFA_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9395407.1"
                     /translation="GLTTLDKTVQEKLLKTNLTVLSNIIEQAIINNVDFVLLAGDNFH
                     QNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERYWFSFPKNVHLFTSETVE
                     TKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYHIGLYHGEPGVSQKGNYA
                     PFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGHTKKEQASTSILLVELSK
                     GSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSKVEDGFSLIEIKLKDYDH
                     LNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTFYTKTPLAASAKLMEQLF
                     QYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAKQLLNQDFLFEEDQE"
BASE COUNT         3223 a         2141 c         1779 g         3547 t
ORIGIN      
        1 ggatttattt atggatatta atgtttttat gaaagcagaa agttagtttc acagactttt
       61 ctttttggtc ttatcttgaa attgagcttc tactcgataa atacgttgac cttttgaaga
      121 aaatttttct tcatactccg tcataatatt gccttcaaat tgactttgat gtaaatctaa
      181 ccatacctgt ttgaggatca tgccatattg agaaaaactt gccagagaat actcaaacaa
      241 tccttgatta tctgttttga aatggatttc tccatttgga cgaagaatct gttcatctac
      301 agcaagaaaa gtcttatatg tcaatcgccg tttttcatga cgtttttttg gccatggatc
      361 ggaaaaattc agatagattt gatctacttc gctgtcagcg aaatactctg tcaacgctga
      421 tccatccaca tgtagaagtt tcaggttagg aagcggttgt tcgatcaact tgtctagtgc
      481 aatcgaaaca acactgacct gcatttctac tccaatataa ttgatttcag gatgggcttt
      541 tgccattcca gtgatgaact ggcctttccc cataccaatc tcaatgtgga ttggatgatc
      601 gttttcaaac aattcattcc attttccctt ccacaatgtt ggatcactaa ttacaaaatt
      661 cgggtgagcc gctaacattt ccgctgctcc cggacgattt cggacacgca ttatttatct
      721 ccattccttt cctctaatta aaaaggtctg gccaaatctc cgcagagttt gggccaaacc
      781 tcagccactg aaaattcgtt ttaattgtaa aatttcttcg ttcatccgtc gatgatcttc
      841 acgtttgcaa tatctggtga tttcttggag caaattcatg gccgcatacc aatgaaattt
      901 ttcaagcatt tcgtcagtcg ggcgtatgcc ataactgatc agccactgat tccagctaga
      961 cagtggcaca taatttccta aaatcgttgc aatatccacc gcagggtctg caaacatgat
     1021 tgaatcccaa tcgactagat ataaataatt tttacatact aaccaattac gatggtttac
     1081 atctccgtgt actactgata agttttcctt atctattgac gggatctgct cacgaagaaa
     1141 acgatggaca atttgcagaa attgattttt ctggagttct tccggtaatt ctttttcata
     1201 tttttgcagc atcttctccg gcgtcatgac acgaccaccg atacgggtca gcatggtttt
     1261 aagagaattt gaatgatgta aatggtacaa aacatcaatc acgtcgtttc tttgaccaat
     1321 ctcattagga gtcagcaagc gaccttccag ccactcttgt gctgtcaaag tatcgccatt
     1381 ccccgttcgt tttgtccaaa caagttttgg tgtaattcct tcccttgaaa gagcagccaa
     1441 catcggagtc gtgtttcgtt taataaagac tttgtcctca tcacgcatgc ctatgtatgt
     1501 ttttcctgtg tcgcctttga tgggacgtaa ccgccactct tggtctaact gaaactccat
     1561 catgcattct cccgtcctct tactctttgt tttgcagtac ttattattct agtcgcctga
     1621 acactacacg tcaagcacat atacgttctt tttcatttaa ttgccattca aaaaaccagc
     1681 ttttctcaaa aaaaatagaa cgaacagcat tcttactgtc aacgctctat ttttcattct
     1741 catttttacc ctacacagct ttgttttcag tctgacatct tcttcaaacg agttggtaca
     1801 tatagtttaa caaagagcat agtaacaatc aaataagccg ctagcggaac ccatcgctgc
     1861 tgcccatcta atacgactgc agcaatcaaa ccaaaaataa ccgacgcgat aattaatacc
     1921 catgtgagta gatactgaat cgcctgcttc ttctgattct tatggattgg ataaagctga
     1981 acgagaacca tatagcggaa ctggttcgct aaaggtaaca gttggaaacc gatcaaatat
     2041 aaaaataaga aaccgataat cagactaaaa taaaagtcga ctactccggc aatcaataac
     2101 ccacctataa ttactaatcg gaaataaaga ccactaaaat cagaacctct tgccaatctt
     2161 cttgcaaaca agtaaagata agtgtttttt gattctcttt taatatgagt caatatagga
     2221 tccaaatagc gtcttctttt gatctttgcc gtaatttcag gaacatctgt gaataaattg
     2281 ataaattgat agatctgatg caacctatgt tcttctacat gcaccatttt ctcccaatcc
     2341 aatccttttt cttgttgctt ccacaaaaac cagtaatata gacttgcctg tactaatgca
     2401 cctgctattc ctgcccatat tagccaataa aatgatagaa acaaaaagat cagagaagaa
     2461 aaaaaccagc ctaatttcca ataacgaata acttgttgat ctagtcgaaa caattctgct
     2521 ctcttgattt gcagatgact gaatttcaaa ctccataata tgatcagata gacgaaaaac
     2581 atcgaaaagg attgctccgt actaaccacc actaatggca tggccaatcc gcatgccaat
     2641 atcaaaaaga tcaacggaaa aatgcaagaa tagcgaaaag ctcgttccaa ataggtttgc
     2701 atttcttttt ctttaggaag taaaaaaaca atatctgctg cctctgcaaa agtagccatc
     2761 ttacccacga ataaacagag catccaaaat acagagatta tgagccctcc aattgcaaac
     2821 ggtggctgca gggttttcaa ccaattagaa taatataatc ccacaccgcc aatcaagaaa
     2881 agacaaacta aaacaaaatg atcgttcaat acataacgca tatatttcat cattttcttt
     2941 tgatgtcttg ctaaacgttt ttgaaaaaaa ctactcatgt gcctgctcct ttgttaaagc
     3001 aatatagatt tcgtccaaag aagaatccgg cagctgaaat tcttttctca acatctcaag
     3061 agaaccgtct gctcgtatct ctccgttatg taagaccaca aaacgatcac aatatttttc
     3121 tgcagtagct aaaatatgcg tagacatcaa aataccggct ccttcttttt tcatttctac
     3181 catcaattca agcaatgcat tgatagctag tggatccaag cctaaaaatg gttcgtcgat
     3241 gatatataaa gaaggctcga tcaaaaaggc gcataagatc atcactttct gtttcattcc
     3301 ctttgaaaaa ttagctggga accagtcaag acgattagat aaccgaaaag tctccaacaa
     3361 cttttctgct cttttcattg ctatatccat aggtatatcg taggccatcg cagtgacttc
     3421 gatatgttct ctcaatgtca attcttcata taaagaagga gtctctggaa tatacccaat
     3481 ttgtttgcga tattcttccg tatttttctt taaggataga tgattgattt ctatcgttcc
     3541 ttttgaagct tccagcagac cgataatgtg cttgatggtc gtacttttcc ctgctccgtt
     3601 cagtccaatc aaccctacta tctctccagg ctgtacatcg aaactgatat cttttaacac
     3661 agggagatgt ccgtaccccc ctgtgacatg atcgataatt aaactcattc tttttcctcc
     3721 gccatttatt tttatttatt ataccacgaa gaaaatgacg gtattttgtt tttttcctta
     3781 caatagcttt aacttatggt aaagttaagc tgaagtattt attctttata gaaaggatgt
     3841 tttatatgga aaactgtatt ttttgcaaaa tcatcaatca agaaatccca agctataaaa
     3901 tatatgaaga cgacaaggtc tatgcttttt tagacatctc tcaagcaacg aaaggacaca
     3961 cattagttgt gcctaaacgt catgtagcag atatttttga atatgatccc gaacttgcag
     4021 gagaggtttt ttcgcgtgtg cctaagattg ctcaagcatt ggaaaaagct tttccagaaa
     4081 tgaaaggtct taatatcttg aataacaacc gtgaactagc ctatcaatct gtttttcatt
     4141 cacatatcca tttagtccct cgctacagca aagaggatga tttttctatt cactttgtca
     4201 atcatcaaga tagttatgga tcggaagaac taaaagctat tcaagaaaca atcgtaaagc
     4261 aggtgtcctg cgatgattaa aaactttttc aaaggactgt tatttggagc tgctgctggc
     4321 actgtcggag gtctgttatt ggcgcccagg agcgggaatg aaacaagaaa gaaactgatc
     4381 gaagaattgg aagaagcaac aactctgaca aatgatttaa ataacagcct gaatcatttt
     4441 aaaagcgcat tgatcgaaac aaaagaaaca gctgaatctg tgattcctgc tttccaagaa
     4501 gcaatcaaaa aagatatcga agattttaaa ttccaagccg aaccacgtat cacacaaatc
     4561 aatgaacaag tagaaaaact aaaagctgat ctgccagagt atcctgaaca aatataataa
     4621 tgattatttt ctcaacagat ttaactatat aagtgagaaa agtttgcgta tttcctagga
     4681 atgtgatgaa attttgaatt ttgtttgaaa ctaccacgac aacgttttta aaggaaatgg
     4741 gatatggtat agttaatata taaaaataat aactaattac aggagtgcaa attttaatga
     4801 aaaaaaaatc aattattttg gcagctacta gtgcgttggc tgttttaact ctagcagctt
     4861 gttcaggcga tactaataaa gatatcgcaa cgatgaaagg cggaacgatc actgtatctg
     4921 atttctatga tgaagcaaaa cttgaatcat cgaaccaatc cttagtacaa cggatgatca
     4981 tttataaagt attcaataac aaatatggcg ataaagtaac cgataagcaa gtggatgcag
     5041 aatacgacaa acaagcgaaa tcattaggtg atacttttga atcacaatta gaagctgctg
     5101 gatatacgaa agatacttat aaagaatata ttcgtaacaa tttagctttt gaagctggct
     5161 tgaaagctca cgtagatatt acagatgacg atttgaaaac tgcttggaaa tctttccatc
     5221 cagaagtaga agcacaaatc atcaaattat caagtgaaga tgaagcaaaa gatgtgaaaa
     5281 aatcagcgga tgatggagat gatttctcta aattagccaa agacaaatca actgatacag
     5341 agacaaaaga agatggcggt aaagttaaat tcgactcaac gacaacaact attcctgcag
     5401 aagtaaaaga agcagctttc aaattgaaag acggtgagat ctccgatgtt attactacaa
     5461 caaatcctac ttcttatgcg acagaatact acgttgtcaa aatggtgaaa aaccaaaata
     5521 aaggcaatga catggataaa tacaaagatc aactaaaaga tatcgctact gaaactaaac
     5581 taagtgacaa tgcattcaca actaaagtaa tcggtgaaga attaaaagat gcaaatgtaa
     5641 aaatcaaaga tgatgccttt gaaaatgtgt tgagtgcatt cacaacaact tcatcaagta
     5701 cgaaagactc aagtgaaact acagcttcaa caaagagttc tgacacaaaa tcaactgatt
     5761 caacaaaaga atcttcaact gaagaaacaa ctgattcatc aaaataaaag aaaacaaaaa
     5821 aagcgaagac ttatgtctcg ctttttttgt tttgaatgaa aaaaataaat ttttgctata
     5881 aatcttttgg tacatagaaa cttcggttgt ccataccgaa aatccgatcg gtgtattttc
     5941 ctggttctgt ttgacgaatc gaagtcaaca tcatctgcat cgaagcatcg atattgtcga
     6001 tttgatgtaa tatttctgct tccattattc ttggacgtac aggtgagcca tattctaata
     6061 agccatggtg tgctaaaacc atatgacgta atacgagaac atcttcgtca gcttcgctga
     6121 ttttcaacgc aagacaggct ttagttattt cttcgtcaat caataccaga tgaccgatca
     6181 aatttcctgc aagcgtatat tctgtcgaca taggaccgga aagttcaatc acctttccta
     6241 aatcatgcaa aatgatacca gcatacaaca atgctcgatt cagctcagga tattcatcgg
     6301 cgattgattt ccctaagtga agcattgtca aagtatgata agctaatcca ctggcaaatg
     6361 catgatggtt tctttttgct gccggaaact caaaaaattg tttttgatat ttattaagta
     6421 gatagcgaac aatacgattc caatgcgcat tggtgatctc aaacaacaat tgattgattt
     6481 cttcttccat ttcttctttc ttcaaagggg ctctttccat gtataaacta ggctctcctg
     6541 gctcatctac ccttgttaag cgcatatgaa gaatctttac ttgagggttt ccttgataaa
     6601 cctctctttt accgtttaga aaaacgactg tgccggctgt aaatttattg atttcctcct
     6661 ccgaagcatc ccagtattta ccatcgatag tacctgaagt atcttggaaa gtaaaagcaa
     6721 taaatttttt tccgttttta gcgattctta catctgcact tttgatcagg acaaattgtt
     6781 caaactgctc atcgattact aaatcacgaa tttttttcat tatttgctcc ttctaattgt
     6841 tttacgattt ggttcatctc atggtaatag cttaccattt ctttatcaga agaaaaacaa
     6901 attatttgat acttttctcc aaatttagct aaaagttcag ctaattgata ctttcgctga
     6961 tgatcataat gaagccagcc atcatcaatg atgatcgggc atatcgaacg gttagcttct
     7021 aatgagaggt aagcaaatct catcgccata atcagctgat ctttcgtacc tgtagataac
     7081 tcatagatgg gaaattctgt atatccgtct gttaaagtca gtgtatcatt tttcagtaat
     7141 atcttctgat aatttccttt tgttaaaata cctaaataat cacctgctgc tcttaacaac
     7201 tggggtaatt gtctttcact catctctgtt gaaagatcac ctaagaatgt cccggcgatt
     7261 tcaagtgctc cccattcctc cagtaattcc tgtaattttg ctttttgccg gctagcttgt
     7321 tgataaagtt cgtccaatgt gccgtctctt tgcaactctt ctacttgaag ttggagtcgt
     7381 tgccgatttt cacttgcttc tttttcttct tgctggagtt gggattgctt ttctttcagt
     7441 tggcgcagtc ttacagataa tttctctttt gtcagctctt caggaaatag tggagtaagc
     7501 atttccgata attcattgta tcttttcatc tgctgttcgg cttgtttggc tttttgtaaa
     7561 aagacaggta cttccgaagg ataagataag cctgcttgag tcaattgttc gctgtattct
     7621 tgtatccata ctttttgttt ctttttcaat tgattgattt cttgcttcaa taaagtattt
     7681 tgctgatagc ttctggcaaa tttcatctgt tccatctctt gtataaaacg ggaaagcaat
     7741 accatttttt ctgaaattgt cttatcttgt atcggtaacc attctttaac aaactcaaat
     7801 ttttggtcga tttgtcgtag tttttcttgt atttcctgtt ctcttttatg tttccgctca
     7861 aatacgccat ataatttctg ataatctatg acttcctgtc catattcttt catgagtttg
     7921 agcgtatcca tctttcctaa gtgccgatga tccgcaaaac gatatacttc ttgctgaaga
     7981 atctgttttt cttctccagt tctcttttgt ttttcttctg cttcagctaa ttgagctaag
     8041 gctagatcaa gttgtcctag tttttcttgc cacattcctt ttatctcaga gaaatcattt
     8101 ttagttttaa tttttggttg ccttttgtag aagaaatagc caatcatgcc tgcaccaatt
     8161 agtaaaagga tcactttcaa gggcattggt acaaatatac ctgcaatgat tattgctccg
     8221 ccagccatca gcatatacgg tttagatgaa gagactggtg ctatctctga ctgattttgt
     8281 aataatgctg gataggtctc ttccaagtca ttcacttctg cttcaatcat ctcttgctgt
     8341 tctttagcta tttggagacg cacagaaatc tggctttcta gatcagcaat ccttctttct
     8401 tcagccagaa gatgcttcca ttcttcttca tataaaaatt cttccggtaa cggatttttc
     8461 tgccaatgcc acctttgttc caaaatcttc aattctgaat ttgtttctct agtctcaatt
     8521 tgtaatcttt gccattcttc gtttaattgt tccgctgtaa attgaagttg ctggagatct
     8581 tttagttcct gctcctgctc taaataaaaa taatatctgg ctgactgctg atccatcccg
     8641 ctatgctttt ccagtgttgc ttctaattct tccaaacgct catttgtttg ctgataccgt
     8701 ttggagaaag actccagatc taaaagaagt tgctcatcag gaagacctgt agtgctgttt
     8761 tttttgagag attgccattc ttcatataaa gaaaaattaa gttcttgctg tctggctaat
     8821 gaaaattgtt tgttcgcctc ttttaattgt gccaataact gctgctgttg cgactcgtat
     8881 tcctgttctt gttggatcaa cttgccaaaa taagcttcct gttcttgttt ttgatgaatt
     8941 ttttcttgca gctctttcca ttcatttaat ttttgattga ttagtaattt ttggcctttt
     9001 ttcttgaata attgttgtgc tttctgataa tattcttgcc gcttttggaa tacttgagaa
     9061 ctgcccgtga tgcctaaaga aatcagcgca tcatgtagct ctttttctct caaatgatct
     9121 agttccatca actgctcttg ctggaaagta aatacctgct ggaataactc tttcgtcaaa
     9181 ggtgcgatca gtttttctag taactcatca cttcccactt gatcccccag ccacaccttc
     9241 gattttccgc gattttgctg cttgtagcgc tcgacagcaa attcaccata tacaggatgt
     9301 ttaagccata ttttgccgcc ataggctgag ccgtcttttg gtgtataatc ctttttttta
     9361 cggccttttg aagggaaacc aaataatatt gcttgaataa attgataaat cgttgatttt
     9421 cctgcttcgt ttcccccata gatcagctga ttgcctgaat aaaagtcaaa acttttttga
     9481 cgccattttc caaaagcagc aatctcgatc cttaaaatct tcattcctga tcctcctcaa
     9541 ataaaaaatc ttgattaagc agctgcttgg ctttttcaag tgtttcttct tgatattccg
     9601 gcagttcgct caatattcta gccgcttctt gatgactata tacttcattt aaaatttgtt
     9661 ggaaatcttg aggtgtctga tagtattgaa ataactgttc catcaattta gcagaagcag
     9721 caagtggggt cttcgtataa aaagtattct ctatgagaga aatacgccaa ataaagaaat
     9781 catttatcct atctgagaat tcctctaaca aatagtctaa tagctctcct gattgtatcc
     9841 gttccaaaac atctgtattg agatggtcat aatcttttaa ttttatctca attaaactaa
     9901 atccatcctc tacttttgaa agctgctgac gaatgtgaac aatgacctct tttgtcgttc
     9961 ttgctttcct caatgatatt tcctttgtct tccaatagac ctctgctact ttaatcggat
    10021 gtatttggca acttccttta cttaattcaa ctagtagaat agatgtagaa gcttgttcct
    10081 ttttagtatg accttgaggc gcacctggat aaacaattgt ttgcttttca tttaagaccg
    10141 ttggcacatg gatatgccct aaagcccaat aatcgtatcc cttttcttgc atctttgagg
    10201 gttggaaagg agcatagttt cctttttgag agacgccagg ttccccgtgg tacaacccaa
    10261 tatggtaatc tgtgagttct ctagaaggaa attccatcac tttgtctttt tgaatccacg
    10321 gttgacgata actaaatcca ctgagagaga cagtttctcc actttttatc gttatttttt
    10381 ttgtctctac cgtttcggat gtaaataaat ggacattttt aggaaaggag aaccaatacc
    10441 gctctttttg atagaaatca tgatttccga atattataaa tacaggtata tggttctttt
    10501 ctaaccgttt catctgttct gaaaaatgtt tctgtatttt taatgaagga cgattttgat
    10561 gaaaattatc tcctgcaagt aaaacaaaat caacgttatt tattattgcc tgttcaatga
    10621 tatttgataa aacggtcaga ttcgtcttca atagtttttc ctgtacagtt ttatcaagag
    10681 ttgtcagtcc
//
LOCUS       DAYYNG010000096        10568 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000096 DAYYNG010000000
VERSION     DAYYNG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.096"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACHBFA_001934"
     tRNA            12..84
                     /locus_tag="ACHBFA_001934"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACHBFA_001935"
     tRNA            88..160
                     /locus_tag="ACHBFA_001935"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACHBFA_001936"
     tRNA            168..249
                     /locus_tag="ACHBFA_001936"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACHBFA_001937"
     tRNA            267..339
                     /locus_tag="ACHBFA_001937"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACHBFA_001938"
     tRNA            351..422
                     /locus_tag="ACHBFA_001938"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACHBFA_001939"
     tRNA            439..524
                     /locus_tag="ACHBFA_001939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACHBFA_001940"
     tRNA            535..608
                     /locus_tag="ACHBFA_001940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACHBFA_001941"
     tRNA            618..691
                     /locus_tag="ACHBFA_001941"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACHBFA_001942"
     tRNA            711..783
                     /locus_tag="ACHBFA_001942"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACHBFA_001943"
     tRNA            811..884
                     /locus_tag="ACHBFA_001943"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACHBFA_001944"
     tRNA            898..971
                     /locus_tag="ACHBFA_001944"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACHBFA_001945"
     tRNA            995..1084
                     /locus_tag="ACHBFA_001945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACHBFA_001946"
     tRNA            1097..1170
                     /locus_tag="ACHBFA_001946"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACHBFA_001947"
     tRNA            1189..1264
                     /locus_tag="ACHBFA_001947"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACHBFA_001948"
     tRNA            1266..1336
                     /locus_tag="ACHBFA_001948"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACHBFA_001949"
     tRNA            1350..1423
                     /locus_tag="ACHBFA_001949"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACHBFA_001950"
     tRNA            1448..1535
                     /locus_tag="ACHBFA_001950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACHBFA_001951"
     tRNA            1557..1628
                     /locus_tag="ACHBFA_001951"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACHBFA_001952"
     tRNA            1651..1740
                     /locus_tag="ACHBFA_001952"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACHBFA_001953"
     tRNA            1753..1826
                     /locus_tag="ACHBFA_001953"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACHBFA_001954"
     tRNA            1845..1917
                     /locus_tag="ACHBFA_001954"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACHBFA_001955"
     tRNA            1921..1993
                     /locus_tag="ACHBFA_001955"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACHBFA_001956"
     tRNA            1998..2068
                     /locus_tag="ACHBFA_001956"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACHBFA_001957"
     tRNA            2081..2154
                     /locus_tag="ACHBFA_001957"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACHBFA_001958"
     tRNA            2166..2237
                     /locus_tag="ACHBFA_001958"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACHBFA_001959"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACHBFA_001959"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9395408.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACHBFA_001960"
     CDS             3826..5298
                     /locus_tag="ACHBFA_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9395409.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACHBFA_001961"
     CDS             5381..6169
                     /locus_tag="ACHBFA_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9395410.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACHBFA_001962"
     CDS             complement(6236..6904)
                     /locus_tag="ACHBFA_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM9395411.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACHBFA_001963"
     CDS             7019..8731
                     /locus_tag="ACHBFA_001963"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395412.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACHBFA_001964"
     CDS             8734..10506
                     /locus_tag="ACHBFA_001964"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395413.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3032 a         1925 c         2449 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataa
//
LOCUS       DAYYNG010000097        10474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000097 DAYYNG010000000
VERSION     DAYYNG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.097"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..2351
                     /gene="pcrA"
                     /locus_tag="ACHBFA_001965"
     CDS             117..2351
                     /gene="pcrA"
                     /locus_tag="ACHBFA_001965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9395414.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2368..4404
                     /gene="ligA"
                     /locus_tag="ACHBFA_001966"
     CDS             2368..4404
                     /gene="ligA"
                     /locus_tag="ACHBFA_001966"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9395415.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4510..4815
                     /gene="gatC"
                     /locus_tag="ACHBFA_001967"
     CDS             4510..4815
                     /gene="gatC"
                     /locus_tag="ACHBFA_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9395416.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4815..6287
                     /gene="gatA"
                     /locus_tag="ACHBFA_001968"
     CDS             4815..6287
                     /gene="gatA"
                     /locus_tag="ACHBFA_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9395417.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6284..7714
                     /gene="gatB"
                     /locus_tag="ACHBFA_001969"
     CDS             6284..7714
                     /gene="gatB"
                     /locus_tag="ACHBFA_001969"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9395418.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7724..8779
                     /locus_tag="ACHBFA_001970"
     CDS             7724..8779
                     /locus_tag="ACHBFA_001970"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9395419.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEARRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRVIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8890..10263
                     /gene="rlmD"
                     /locus_tag="ACHBFA_001971"
     CDS             8890..10263
                     /gene="rlmD"
                     /locus_tag="ACHBFA_001971"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9395420.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRTGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGVKPSVIVVDPPRKGL
                     DHRFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            10266..10394
                     /locus_tag="ACHBFA_001972"
     CDS             10266..10394
                     /locus_tag="ACHBFA_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9395421.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         3636 a         1885 c         2325 g         2628 t
ORIGIN      
        1 acgaattgac aaggaaaaat ccataaaaag aaacaaatga ttttgaacta gctagtttaa
       61 tgaaagccaa gaaaatgcta taattaacaa gattgataaa agaaaggatg ttgttaatgt
      121 catccaaagc tgaattatta aacgggatga acccccgtca gaaagaagct gttcttcaca
      181 ctgacggacc attactgcta atggcaggtg caggaagtgg aaagacgaga gtgctgacac
      241 accgcattgc ctatttgatt gaggaaaaag aagtcaatcc ttggaacatc ctagcgatta
      301 cttttaccaa taaggctgca aaagaaatga aagaacgtgt caatgcgatt ctcgcatctg
      361 gaggcgaaga tgtctgggta tcgacttttc actcgatgtg tgtgcgtatt ttaagaagag
      421 atgttgattt tatcgggtat aaccggaact ttacaatcat cgattcttct gaacagctga
      481 ccttgatgaa acggatacta aaagaactga acattgatcc gaaaaaatat gatcctcgaa
      541 gtatattagg aacaatctct caagcaaaaa acagcctgca gacccctcag gattttgcaa
      601 aaatgcaagg cagttattac gaagagattg cggcaaaatg ttatgctgct taccaaaaag
      661 agcttcaata taatcaatgt atggactttg acgacctgat catgaatacg attcgattat
      721 ttgaagaaca tcctgattca ctcacatatt atcaaaacaa attccattat attcatgtag
      781 atgagtacca agatacaaat cacgcacagt atacgctagt caatttattg gcaggtagat
      841 ttaggaacct gtgcgtcgtg ggagatgcag atcaaagtat ttacggctgg cgcggtgcag
      901 atatgcagaa catcttggat tttgaaaaag attatccaga tgctgcagta attttgctag
      961 aacaaaacta tcgctccacg aaaaacatcc ttagcgcagc aaaccaagtc attgaaaaca
     1021 atagcaatcg aaaaccaaaa aacttgtgga cagaaaacaa agaaggaaac aagatcactt
     1081 actaccgtgc agataatgaa cgagacgaaa cccgttttat cgtagatcgg atgcaagaag
     1141 agattcgaag caatcatcgg aattacggcg actttgccat cttgtaccgg acgaacgcac
     1201 aatctcgtgt aatggaagaa actctgttga aagcaaatat tccttataaa atggttggag
     1261 ggcacaagtt ctacgatcgt aaagaaatca aagatatttt agcgtatttg aatgtgctgg
     1321 caaatcctca agattcaatc agttttgagc gtatcgttaa ttcgcccaaa cgaggcatcg
     1381 gaccaggatc tatcgaaaaa ctacgcagtt ttgcttctct tcatgaatgg ccactgcttg
     1441 aagcagcgca aaatgtggat ctggcaaata tcggtggaaa agcaggacag caattaggtg
     1501 catttggaga gatgatccaa gaagtcactc agatgatccc atacttgaca gtgacagaat
     1561 tgacgaaaga ggttttggat cgtagcggat atctagaaga cttaaaaatc caaaatacat
     1621 tagaagcaca agcacgcatc gaaaacttgg aggaattttt aacggtcaca caagaattcg
     1681 acaaacaatt cgaacagcaa aatgaagaag acgctgatgc accagaagaa aaactaacgg
     1741 tcttcttaaa tgatctggca cttgtttccg atattgacaa tttagaagaa gatgcttccc
     1801 aagtgacatt gatgactttg catgcagcca aaggattaga attcccagtt gtattcctga
     1861 ttggtctaga agagggggtt tttcctttat ctcgagcatt gatggaagaa agcgaactgg
     1921 aagaagaacg ccgtctcgca tatgtgggga tcacgcgagc agaagaagcg ttgtatttaa
     1981 caaatgcttt ctccagaaca ttatatggac ggacgcaata taaccgacct tctcgttttg
     2041 tagaagaaat cgatcaggag ttactggaaa tcgaaggcat gcgtcctacg cctaaaaaaa
     2101 cacctgtatt cgctaaaaaa acagcatata gctataaaca gccagagaca gcggttgttc
     2161 cctctaaaag tgctactgga ggggaaaata acagctggaa acctggagat aaagtcaaac
     2221 acaaaaaatg gggtctagga acagtcgtcc gagtaagcgg gacgtcgaag gatctagaac
     2281 tagatgtggc tttcccaagt caaggagtaa aacgtctttt ggctgccttt gcgccaattg
     2341 aaaaagctta gtagtggagg gtaaatgatg gaacatgaaa tgacgattga cgaagctgct
     2401 aaacgagcag aagaacttcg tacaagactg aaccaatggt ctcgcgaata ctacgtagaa
     2461 gataaaccaa cagtggaaga ctatgtttat gataaagaat acgcagaatt agtagcaatt
     2521 gaggaacaat atcctgattt gatcacgtct gattctccca ctcaacgagt aggcggaaaa
     2581 gtattagaag gttttgaaaa agtcactcat gatattccgc tttacagttt gaacgacgtt
     2641 ttcagcaaag aagagctgat agcttttgat caacgtgtac aaaaagcagt gggacgtgta
     2701 gttgactatt gctgtgaact aaaaatagat ggactttccg tttctttgcg ttatgaagat
     2761 ggaaatttcg tgagaggtgc gactcgcggg gatggaactg taggagaaaa catcactgaa
     2821 aacctaaaaa ctgtccggtc ggtcccaatc aaattaaaag aaccaatgaa catcgaagta
     2881 agaggcgaat gtttcatgcc aaaacggtca tttgtccaat tgaaccaaga cagagaagca
     2941 gagggaaaag atatctttgc gaatcctcgg aacgccgcag ctggttcttt gcgtcagtta
     3001 gattctaaaa tcacagcgaa aagaaatctg gacacttttt tatacacagt agctgatttc
     3061 ggtcctatgc aagcaaaaac ccagtatgat gcgctagaag agctagaaaa aattggattc
     3121 cacacgaatc gggaaaaacg tctatgtcat tcgattgatg aagtgtggtc atacattgaa
     3181 gaatatcatg acaaacgtgt ggatttgccc tatgaaatcg atggaatcgt catcaaagtg
     3241 aatgaattct cgctacaaga tcaacttggt tttactgtca aagcaccgag atgggcagca
     3301 gcttacaagt ttccgccaga agaagttgaa acgctaatcg aaaacatcga atggacagtc
     3361 ggtcgaacag gggtagtaac gccaacagct atcatgacac cagtccgagt agcaggaacg
     3421 acagtaagcc gtgccagcct gcataacggt gattatatta agttgaagga tatccgcttg
     3481 aaagatacag ttctgattta taaagcggga gacatcatcc cagaagtttc gcaagtcgtg
     3541 ttagataaac gacctaaaga cagcgaagaa taccaattgc ctacacattg tccagtttgt
     3601 ggaagtgaac ttgtccattt ggatgaagaa gtagccttac ggtgcatcaa tcctaaatgc
     3661 cctgctcaaa tgaaagaagg tttgaaccac tttgtttcaa gaaatgcgat gaatatcgat
     3721 gggttggggc cgcgcgtact agaacagatg tatgataaaa aactagtagc agatgttgct
     3781 gatctttaca aattgacaga agaagagtta ttgacgttgg acaaaataaa agaaaaatca
     3841 gcaaataata ttttgacagc aatcgacaac agcaaagata attcggtcga gcggttgatt
     3901 ttcgggttag gaatccgaca tgtcggagcg aaagcagcaa aaattttagc agaacatttc
     3961 ggtgatctag aaacattgag taaaagtgac tatgagtcga ttattgcgct tgatacgatc
     4021 ggtgatatca ttgccgacag tgtagtcact tatttcagta acgaagaagt ccatgaattg
     4081 atgaatgaat taaaacaagc aggcgtcaat ttcgaataca aagggcttcg aaatgcccaa
     4141 ttgcaagaag tcgagtctcc ttttaaagaa aaaacagtag tccttactgg taaactaaca
     4201 cgatttactc gtgaagaagc aaaagagacg atcgaaaatc ttggcgggaa agtgactggt
     4261 agcgtatcga aaaaaacgga tatcgtggta gcaggagaag atgctggcag caagctgacc
     4321 aaagcacaag aattaggaat cgaagtttgg acagaagatc aaatggcaga tgcattggct
     4381 aaaagtcgct cagtagaaga atgaaaaatg agttttttca gacaaatttc aataaacaat
     4441 ttcagacacg gcactcttgt gttaaaatag agagcaaagt ttttaactcg tttagaaaga
     4501 aggggtagta tggcgattag tgaagaacaa gtgaaacacg ttgccaaatt agcaaaactt
     4561 gcgttcgcag atgaagaact tgcaagtttt accgaccaat taggtaaaat catcgatatg
     4621 gttgaaatgc tggaagaagt agatacagaa ggtgttccat ttacgtcgaa tgtggttgaa
     4681 acagtaaacg tgatgcgaca agatcgggcg caagctggat ggaaccgcga tgaattattg
     4741 aaaaatgtgc cggaaacaga agacggcttt atcaaagtac ccgcaatcat cgataatggg
     4801 gaggctggcg cataatgaca gaattacaca atcgttctct agaagaactg catcatatgc
     4861 ttgtatcgaa agaaatcact gtacaagatt tgacaaaagc aacatttgaa cgaatcaaag
     4921 aaacagagcc tgaggtcgaa gcattcatta cattgaacga agaaaaagca ttggaacagg
     4981 caaaagcttt agatgaaaaa gggattgatg aaagcaacgt ccttgctggt attccaattg
     5041 gtatcaagga taatatcgta acaaaagata tcttgacaac cgctgcatct aagatccttt
     5101 acaattttga accaatttat gatgcaacgg tcatggataa aatctatcaa gcagatatga
     5161 tccctgttgg taagctaaac atggatgaat ttgctatggg tggaagtaca gaaacttctt
     5221 atttcaagaa aacaaaaaat gcttgggacc caacaaaagt acctggtggc tcttctggtg
     5281 gttctgcagc agcagttgca gctggacaaa ttccagtttc tttaggtagt gatactgggg
     5341 gaagtatccg ccaaccagca gcttttaacg gcatcgtagg gatgaaacct acttatggcc
     5401 gtgtctcacg ttttggtttg attgcctttg cttcatctct tgaccaaatc ggaccaatga
     5461 cccgtacggt gaaagataat gcattagcat tgacagctat cagcggttat gacgaaaaag
     5521 acggtacttc ttcaggcgtt tcagttcctg attttgcaga gggactgaca ggcgacatca
     5581 aaggaatgaa gattgcttta cctaaagaat acttaggcga aggtgtggcc cctggcgtaa
     5641 aagaagctgt attgaaagca gcagaaacat tccgttcatt aggtgctgtt gtggaagaag
     5701 tcagcttgcc tcattcaaaa tacggtgtgg ctgtttatta tatcattgcc tcatcagaag
     5761 caagttccaa cttgcaacga ttcgatggta tccgctacgg ttatcgctca gaaaatgtga
     5821 aatcattaga tgatgtatat gtcaactcac gttcagaagg attcggtgaa gaagtaaaac
     5881 gccgtatcat gttaggtaca ttctccttaa gtgctggtta ttatgacgct tacttcaaaa
     5941 aagcgggaca agtccgtaca ttgatcaaac gagattttga aaaagtcttt gcagattatg
     6001 acttgatcat cgggccatct tcaccaacag ttgcattcgg aatcggtgag aacattaacg
     6061 atccaatcac aatgtacatg agtgacatct taacgatccc tgtcaacttg gctggacttc
     6121 caggtatgtc tgtaccggca ggtttatcag aaggattacc agttggattg cagatcatcg
     6181 gtaattattt tgatgaaaaa acgatgtatc aagcggcata tgcgtttgaa caagcaacag
     6241 aattccataa acaacaacca gctattttag gagggaaaga cgaatgaatt ttgaaacagt
     6301 cattggacta gaagtccacg tcgagttaaa aaccaactca aaaattttct ctccagcgcc
     6361 agctcatttt ggtgcagaac caaacagcaa tacaaacgtg atcgactggg gttatccagg
     6421 cgtgctgcca gtaatgaata aagctgcgct ggaattcggg atgaaagcag ctttggcatt
     6481 aaactgtgaa atctctaaag atactcattt tgaccggaaa aattactttt acccagataa
     6541 tccaaaagca tatcaaattt cccaatttga tcaaccaatc ggtcatgacg gatggatcga
     6601 aatcgaagta gaaggtaaaa agaaaaaaat ccggatcgaa cgtgtccatt tagaagaaga
     6661 tgctggaaaa aacatgcatg gtattggcgg ttattcttac gttgatttga accgacaagg
     6721 cacgcctttg atcgaaatcg tttctgaagc tgatatgcgt tcaccagaag aagcatacgc
     6781 ttatctagaa gcattgcgat caattatcca atttacagaa gtcagcgatg tgaaaatgga
     6841 agaaggctct atgcgctgtg atgccaatat ttctcttcgc ccttatggac aagaagagtt
     6901 cggtacgaaa gcagaattga aaaacttgaa ctctttatct ttcgtgaaaa aaggtttagc
     6961 ttttgaagaa aaacgtcaag caaaagttct tctttcagga ggagaaatcc aacaagaaac
     7021 acgccgtttt gacgaaacaa cgaataaaac agttttgatg cgtgtcaaag aagggtctag
     7081 tgattatcgt tacttcccag agcctgatat cccgaaattc gtgatcgatg acgaatggat
     7141 tcaaaaagta aaagctagtt tgcctgagat gccagcttct cgtcgcgaac gctatatccg
     7201 tgagtttggt cttccagagt atgacgcaat ggtattgaca cttacaaaag aaatgtctga
     7261 tttctttgaa gctgttttgg aaaatggtgc agatgccaag caagcatcta actggttgat
     7321 gggtgaagtt tctgcttact tgaacagtga gaaactggaa ttagctgaga caaaattgac
     7381 acctgagaac ttagcaggaa tgattcgttt gatcgccgat ggaacgatca gctcaaaaat
     7441 cgccaaaaaa gtcttccgtg aattgatcac aaatggcgga gaagcccaag aagtagttga
     7501 aaaaaatggt tgggtgcaat tatctgatcc gaacaaacta ctgccaatca ttaatgaaat
     7561 tttggataac aaccaacaat ctgtcgatga tttcaaaaat ggaaaagatc gtgcggttgg
     7621 cttcctagtc ggtcaaatca tgaaagcaac taaaggacaa gcaaatcctg gtgtcgtgaa
     7681 taagctactt aaagatgaat tagcgaaacg atagaggctg aaaatgaaaa aagcacgcgt
     7741 aatttataac cccacctcgg gaaaagaatt attgaaaaaa aatttggcgg atatcctaca
     7801 ggcattagaa gaagcaggat ttgaagccag cgcctatgca actacaccag aaccagattc
     7861 agccaagaat gaagcacgta gagtcgcaga actaggtttt gatctagtag tggcagctgg
     7921 tggcgatggc acaatcaacg aagtggttaa tggaatcgct ccgcttgaac accgtcctaa
     7981 aatggccatc atcccagctg gtacaacgaa cgactatgct cgagcattaa aaattcctag
     8041 agataatatt aaagcagcag cagaagtgat taaaaagcat caaaccgtaa aaatggacat
     8101 cggcctttct gataacagtt acttcatcaa tatcgctgca ggtggctatt tgacagaatt
     8161 gacttatgaa gttccatcag aactaaagag tatttttggt tatcttgctt atcttgcaaa
     8221 aggagcagag atgctccctc gagtaaagcc catcaaaatg cgcttgaagt atgatgaagg
     8281 ggaatatatc ggaaatgctt cgatgttttt cctagggctg acgaactctg ttggaggatt
     8341 cgaacagatc gcgccagatg ccaaattgga tgatgggaag ttttctttaa tcattgtgaa
     8401 gacagcaaat atctttgaga tcttgcatat tgcggcactc atgctgaatg gcggtcgaca
     8461 tgtcgatgat ccaagagtaa tctataccaa aacaagcagt ttgttcgtcg aaacagaaga
     8521 tgatatcaat cgaccagtaa tgatcaactt agatggtgaa tatggtgggg aagcaccgat
     8581 gcatttccaa aatcttcatc agcatattga gttctttgcc aataccgatc agattcctga
     8641 tgaagcaatc acaggcacag atgaagaaga attagaggtt gttagcaaag aatttgtaaa
     8701 agaagtcgaa cgtttaacgg atgaagatat cgacggtgac ggaaaaatag cagacgaaac
     8761 acctaatcaa aagaattaat caaaagccgg tgctgaacgt agtaaggtca gtgccggttt
     8821 tagttgtgta attagctgat ttatagtaga gttcagtagg attgaaaaat aggcttaaag
     8881 gagcaaaata tgacagaaca gcaaatagta aagaaaaacg aacgatatac agtcgaaatc
     8941 gtcgacttga gttatgaagg aatgggggta gcaaaaatcg atggttatcc attatttatc
     9001 gaaaatgcac tccctggaga agaagtagaa atactagtag taaaagcagg caataagttt
     9061 ggatatggaa aagtcgaaac atttattaca caatcacctg atcgtcagcc tgtagaaaac
     9121 aatgcattac ttcgcacagg tatcgcacct ttagcccatt tgaagtatga acaacagctt
     9181 gtcttcaaac aaaaacaagt agagcatgta atggcgaaga ttgctaagat gccagaagtt
     9241 cctgtacttc caacggtcgg aatggcagaa ccatttggct accgtaataa agcacagatc
     9301 cctgttcgcc gtatcgatgg acagttagcc accggttttt acaaaaagaa tagccatgat
     9361 ctagtcccta tcgaagattt ttatatccaa gatcctgtca tcgatcaagc aatcaaagta
     9421 gtcagagata tcttgcaacg gttccaagta cgaggatata atgaagaaaa aaatgaagga
     9481 cagcttcgtc acatcattat ccgtcgtggt cattattccc atgaaatgat ggtcgtttta
     9541 gttactcgta aagaaaaatt ctttaaatcc aaggaaattg ctgaagccat tcatgaagaa
     9601 ttgccagaag tggtttcggt catccaaaat atcaatgaag aaaaaacaaa tgtgatttta
     9661 ggagacaaag aaaaagtttt atacggcaga gactatatcg aagatcagct attaggaaaa
     9721 acctatcgaa tctcagcgaa gtcattttat caagtaaata ctcaacaagc agagtatttg
     9781 tatcaaacag ccatcgaatt tgccgagttg gataaaaaag acattgtgat cgatgcttat
     9841 tcaggaatag gtacaatcgg attgtctcta gctgacagag tagataaggt atatgggatg
     9901 gaagttattc cagaagcaat tgaagatgca cagttcaatg ccttaaataa taaaatcgaa
     9961 aatgttcatt atgaagtcgg aaaagcagaa aaagtcatga aaaaatggca agaaaaaggg
    10021 gtcaaaccat ctgtgatcgt agtagaccct ccacgtaaag gattagatca tcgttttatt
    10081 gaatcagcaa tcgaaatgtc accagaacga atcgtctata tctcttgtaa tccagcaact
    10141 tttgcaagag atgctaagtt atttgcagaa gcaggttacg ccgtgaagaa agttcagcct
    10201 gtagatcttt tcccgcaaac ccatcatatc gaattagtcg cttcattcgt taaaggagaa
    10261 taaaaatgga ccaaaaagaa aaaatgaaag aattgatcaa aaagaaacaa ggtggaggcc
    10321 gatccaaaca aatggaaaca cctaaaaatg atcgtaaaaa catgagaaaa ggccctaaaa
    10381 tctttaataa atagacgtgc taaagaaaga ctaagaagcg ttaacttttg ttattgtttc
    10441 ttagtctttt tagttagtct acattatttt ggcg
//
LOCUS       DAYYNG010000098        10246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000098 DAYYNG010000000
VERSION     DAYYNG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.098"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..113
                     /locus_tag="ACHBFA_001973"
     tRNA            43..113
                     /locus_tag="ACHBFA_001973"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Cys,seq:gca)
     gene            134..218
                     /locus_tag="ACHBFA_001974"
     tRNA            134..218
                     /locus_tag="ACHBFA_001974"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Leu,seq:caa)
     gene            280..352
                     /locus_tag="ACHBFA_001975"
     tRNA            280..352
                     /locus_tag="ACHBFA_001975"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:His,seq:gtg)
     gene            364..435
                     /locus_tag="ACHBFA_001976"
     tRNA            364..435
                     /locus_tag="ACHBFA_001976"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:396..398,aa:Gln,seq:ttg)
     gene            453..537
                     /locus_tag="ACHBFA_001977"
     tRNA            453..537
                     /locus_tag="ACHBFA_001977"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:487..489,aa:Leu,seq:caa)
     gene            792..1667
                     /locus_tag="ACHBFA_001978"
     CDS             792..1667
                     /locus_tag="ACHBFA_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9395422.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1771..2535
                     /locus_tag="ACHBFA_001979"
     CDS             1771..2535
                     /locus_tag="ACHBFA_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM9395423.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2522..3964
                     /locus_tag="ACHBFA_001980"
     CDS             2522..3964
                     /locus_tag="ACHBFA_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395424.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3975..5306
                     /locus_tag="ACHBFA_001981"
     CDS             3975..5306
                     /locus_tag="ACHBFA_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM9395425.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            5319..5777
                     /locus_tag="ACHBFA_001982"
     CDS             5319..5777
                     /locus_tag="ACHBFA_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9395426.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRYAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            6069..7370
                     /locus_tag="ACHBFA_001983"
     CDS             6069..7370
                     /locus_tag="ACHBFA_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395427.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            7397..7657
                     /locus_tag="ACHBFA_001984"
     CDS             7397..7657
                     /locus_tag="ACHBFA_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9395428.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(8656..9891)
                     /locus_tag="ACHBFA_001985"
     CDS             complement(8656..9891)
                     /locus_tag="ACHBFA_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9395429.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3209 a         1582 c         2145 g         3310 t
ORIGIN      
        1 ctataattta ttctttattt tatagattga taatagtata atggcggtat agccaagtgg
       61 taaggcagag gtctgcaaaa ccttcatcac cggttcaaat ccggttaccg ccttagtact
      121 aataaatcaa cttgccggcg tggcggaatt ggcagacgcg ctggactcaa aatccagtgc
      181 ccgttgaggg cgtgccggtt cgaccccggc cgccggtatt gactaagatg tcttcatctt
      241 agttttttat tgagaaatta aataacatct tttatgatgg cgaatgtggc gaagtggtta
      301 acgcaccgga ttgtggctcc ggcattcgtg ggttcgattc ccatcattcg ccctttttat
      361 tcttggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      421 atccagctac cccagttatt atttttaaat ttgccggcgt ggcggaattg gcagacgcgc
      481 tggactcaaa atccagtgtc cgttgaggac gtgccggttc gaccccggcc gccggtataa
      541 ttatcaatat agcaactttc ttatagagag ttgctatatt ttttttgttt ttatgtaatt
      601 acctccaaat caattcgata caagtattat cataaaacga gtaaaaaaga tattgataac
      661 gcttttatta tgtgttaaac tatttttgta aattatgtag ggtgctacta ttcaattagg
      721 cacgacctaa gaaagggttc tttcttaggt cgttttttgt ctttaaaaat tgtttggaga
      781 caaaaatatg tatgaaggag tctgcaagtt gttttgtttt atcatataga ggaggtgaat
      841 taacggtgta tgtgatgaaa aagctgaatc ataatgctgt attgatcgat gacgatggaa
      901 tagaaaaaat tgcgatgggg aaagggattg gcttttctgc taaagtgagt aagccatttg
      961 atgttgagga tgctgacaaa ttatttgtat tagattctaa agaaaagatc aaacgattca
     1021 gtgagatagt ctcgcagatt ccaatcgaat ttatggattt tgcggaagaa gtgattgaac
     1081 tgataacagc taggatctcc tctccattag attcaaatat ctatattaca ttgacagatc
     1141 atatttattt tgctattcaa cgaaaaagag aagatagaga tgtcacagca attatgttgc
     1201 cagaaatgag attcctttat ccaaatgaat atgatatagc agtagaagtg gtcgagcgaa
     1261 tcaatcatcg atatcaaacc gagttagggt caaatgaagt aggattcatc actatgcata
     1321 tcattaatgc tgagctagga gagcctaata gtttaaagag cttaaaaata gtagaaatga
     1381 cgaccacgat tttgtcccat ctagaaaaat cctattcaaa cgtttatgat aaagaatcac
     1441 tgacttatca tcgcttaatg gttcatatca aattcctggt taaacgcttg atttatcagg
     1501 aagaaacgcc agtagaaaat attggttttt ttactgaaac atttaaacat agcaaagcgt
     1561 attgtacagc actaaagatt aaagatatca tcgaacagca atttcaactt gccattcaag
     1621 aaaatgaaat catctatttg gcaattcatc tagcaagaat ctattaaaat aagggcgtaa
     1681 ctatctatag gcgcaaccaa atgacacata gtaaatggac tatgattatt tggttgcgcc
     1741 tattttagta gaaaaaagaa ggagaatgaa atgggaaaag tgatttttaa ttcggatgat
     1801 tttggctata gccatggtgt gaattatggg attatggatg cttatcagcg aggaattttg
     1861 acatcaacta cattgatggc aaatatgccg ggtttcgagc atgctgtgaa actaagaaag
     1921 gaaatgccta ggctaggtgt aggagtccat ttaacattga cgtgtggcaa accactcctc
     1981 aaaactgtcg atactcttat cgaaagcgga gagttcagat ccttatctta ttatcagcat
     2041 ccatttaaca ttgacaaaga tcaactgtat caggaatgga atgctcagat tcaaaaaatt
     2101 tatcatgcgg ggatcatacc aactcattta gacagtcatc atcatactca tactttggat
     2161 gaaaatcagg aggtagttgt tacattagca aaaaaatatg atttgccggt tcggagcaac
     2221 tttgaacgaa aagatgaagt tcgtcatgtc gattattttg agccttgttt cgatgtcgta
     2281 ggagaaactg atgaaaaaaa acgacaaatc aatacgagtg tggaagaata tttggcaagt
     2341 ttactcatga tattgagaga agataaaacg atcgaagtca tgtgtcacgc agcgtatttg
     2401 gatcaggaat tgttaaatgg ttcaagtttt gtgtatcctc gtacccacca agtcgaattt
     2461 ttaattcatt cagcatttgc tcaaaaagtg aaaaatgatc ctttgattga gttagtaaca
     2521 tatgaggata tttaatagag gaggaataaa aatgaatgtt aaatcaaatg tgcagaagct
     2581 tggaagagcg atgctacttc cagttgcagc gatgccatta gcgggattga tcatgcgttt
     2641 atcagctgac gatatgctga acatcccagt aattggtgca gctggaaatg ccgtttttgg
     2701 aaacttggat atcttatttg ctataggagt tgcaatcggt tttgtcaaaa ataaggacaa
     2761 aggaatacct gcacttactg gttttttagc gattgctacc ttgaaaaaag gcttagaaat
     2821 tatggaccca gaagtgaata tggggatttt tggtggaatt atttccggtt tagtagcggc
     2881 atggacgtat aatcgcttta aagaacaaaa attaccagta gttttttctt attttgcggg
     2941 cgaaaaattc ccattgacta tggtgatgat tctacaaaca atcgcagctg tttttttggg
     3001 ctttatctgg ccgctcatcc aaattgggat tgataatttt gcaaaactgc tcgttgatat
     3061 gggagcatta ggtgtgggga tatttatgtt tttgaatcgt ttgctgatcc cctttggact
     3121 gcaccatgtg ttgaatacgt atatttatta cgatttagga agctatacat ctcctagcaa
     3181 ggaagttttc agaggagaga tgacacgctt tatcaacgga gatcctcagg ctggactgtt
     3241 tttatcaggt ttttttgtag taatgatgtt tggtatacca gctatatgtc tagctatcta
     3301 taaagcagct tataaagaaa ataaagagat ggtcaaagga atcatgggaa gcggtgcagg
     3361 aacttcattt attgcaaata tcactgagcc tgttgaattt agtttcatgt ttctttctcc
     3421 tatgttgtac gtgatccatg cactttttgc cggtttagca gggattgtct gttacttatt
     3481 caatatacga attggtttta cattcggagc atgtattgtt gattatctca tcaattttcg
     3541 aatcgctacc aatgctattt tgattcttcc aatcggaatt ttcttttttg ccttgtatta
     3601 cgttactttt tattatttga tcaataagag aaacattcag acattaggac gtgaagctaa
     3661 ggcagagttt ggcaatgaag taaccttaga agaaacagag ttagggttag caagtaaaaa
     3721 ttatgaatat atggcaacaa aaatgctaca agcgtttgga ggaaaagcca atatcctgga
     3781 tgtctatagt tgtaatacac gcctacgggt agaggtagtg gatcccacca tggtcgagga
     3841 acagcgaatc aagcaattag ggataagcgg gatcattaaa ccaactgaaa aaaattatca
     3901 aattatcatc ggcttagaag tgacttatgt tatggcagaa ttcaataaat tattagaaga
     3961 atagaaagga aaagatgatg atgaaaaaac agattatcac tatagctgga ggcgggagta
     4021 cgtatacacc aggaatcgtt caagccattt tgaacaatga aaaacggttg ccattatctg
     4081 aaatacgttt atatgatatt gatgaagaaa gaaatatgga tatgtacttg attgttaaat
     4141 ttatgctgaa aagaaaaggt ttttcaaata ttcgcattcg tgcaacggat gatccaaagc
     4201 ttgcttttac aggatgcgat tttgttttct cacaaattcg tgttggagga ttggagatgc
     4261 gagaaaagga cgaaaagatt ccacttaaac atggattagt aggacaagaa acgtgtggac
     4321 tcggaggatt tgcttatggg atgcgctcaa tcaaaggttt attggaaatt gtcgatcata
     4381 tacaagacta tgcccccaaa gcatggattt tgaattatac aaatccagag tcgatcgttt
     4441 ctgaagctgt tcgtcgtaaa tatccgaatg taaaaatgat taatgcctgt gatatgacta
     4501 tttcaattga agaaacgatt gcagtgaatt acggttatga tcgtaagaat tggatcgtta
     4561 cgtattatgg tttaaaccac tttggctggt atacatcgat ttatgataaa gaattgcaac
     4621 gagaaattat gccagagatt attgaaaaac taataacaaa agaaatgcaa gtcgccgatt
     4681 ttaatatagg agataaaact tggcaaaaaa cctttcaaat gatgtcagtc atcacgaaaa
     4741 attttccaag taatatcccg aataattact tagagtatta cttatatcct gatatggtcg
     4801 ttgagcatac tgacaaagag tatacacgtg ctaatatggt catggatgga cgagagaaaa
     4861 ataccaaaga aatggctgat aaaatacgcc gcggtattgt ggaagaagta ttgaacttta
     4921 attttgggga gcatggacaa tatatcgtcg atatagctat ttccttgttg aatgatgatc
     4981 gtcgtcgctt tatgctaatc gttcctaatc aaggggcgat tccgaatcta cggtcagatg
     5041 cagttgttga aataccggca tatgttggtg caactggtgt ggagccaatt acactaagaa
     5101 aaccaatcag tgattttcat aaaggattga tggaggcaca agttgcagta gagaagttac
     5161 tagtagatgc ttactttgaa ggttcttatc aaaaagcttt acaagcattt acattgaatc
     5221 aaaccgtacc aaatgccaga gtcgcaaaaa aaatactaga tgaaatgatc aaagtaaaca
     5281 aaaaatattg gccagcatta aagtagaggt gataaaaagt gcatttcttt aaaacaagaa
     5341 acattctaaa agctgtagct agtggtcaaa ttattccatt aacagcagta aaagacgagg
     5401 tcttttcttc aaaaatgata ggagttggat acgctataac gaatcatgat ggatatatcc
     5461 atgcgccagt taaaggaata atcgataata tatttcctac ccgatatgcc ataacgatcg
     5521 aaagtgaatc tggcagaaaa atactgatcc atatgggttt agatacagtt gaactcgaag
     5581 gaagtccgtt tcagatacgg gttgaaagta accaggaggt aaaagaaggg acactgttag
     5641 ctattataga tttgaagaag ttggagcaag cacaaaaaga tacggtgatc attgtagttt
     5701 ctccaggcat ttttgaagga aatttattaa aacaaaacca agaagtaaag attaaagata
     5761 gttgttttga attttagtgt acgatgaaaa aagttaaata gggtgatatt tatgggtgaa
     5821 tttacaaaat ttttttgaaa tgatatgttt caatcactag atttaatcat tttgattttt
     5881 gttaattatc gaattttttt gttgctccta tgatttagaa aacttatttt caggattctt
     5941 aaaaataaat ttgattattt aagagaatat attgaaataa aatttcttaa ttatataatt
     6001 agttatgaac attctttgcg ttgtcatgca atgaatggtt catattttat tgaaaaggag
     6061 tggttttagt ggaaaagctg atgaaatggg tcgaagagaa acttgtccca ccaatggcaa
     6121 aaattggtac acagcgtcat ttattagcaa ttcgtaatgg ggttgtttca acattgtcat
     6181 tgattttgat tgggacattt tttatggtct ttatcaattt accgtttcct ggatggaatg
     6241 aatttattgc cccttattcg gcaacgattg ttttaccctt taggatgacg atgggattga
     6301 tggctattta tgcaactttt gttatgggtt cggatctagc taaatcatat ggattagata
     6361 gtgtgacagg tggtatatta tctttaggaa cgttctttat gttacaagtt cctgtaaacg
     6421 ttttaactcc tgaagaggca cctcttggct gggtcttacc tatgagttct ttgggagcat
     6481 ctgggatgtt tgcggggatc ttatctatga tttttgcagt ggaagtatat cgtttcttta
     6541 aaaatcgaaa tattactatc aaaatgccag aacaagtgcc acctgctgtt gcccgtagtt
     6601 ttgaggcatt gattccaggt gcagtcattt taacaattac ttggctgatt cgaagcgtcg
     6661 cgggctttga tgtaaacgct gccttattat cgttattcga gcctttaacc aatattttag
     6721 ggaataattt gcttggtgtt ttactaccaa tgtttttgat ccatttgttg tggtcatttg
     6781 gtatccatgg catgagtatt gtaggggctg ttgttcgtcc aatgtggctg atcatgttgg
     6841 atgagaatgc aaaggcattg gcagatggta cagctgccac ccaattaccc tatatcgctc
     6901 ctgaacagtt ttatcaatgg acggtaacaa ttggtggtgc aggagcaacg atcgtggtat
     6961 ctgttttgtt tatattcttc tgtaaatcaa aattcttgaa agaggttggt cgtttttcta
     7021 ttattcctgg tatcttcaat atcaatgagc ccataatttt tggagctcct atgatgttga
     7081 atccttacat gttcatcccg ttcaacttag ttcctttagt tttgactatt atcagttatt
     7141 ttgcagtcaa attagaaatg gtcaatagat ttactgtttt gccaacatgg acattaccag
     7201 ctccaatcgg cggttatctt tcagcaggaa atgactggcg tgttgttatc ttgatcatga
     7261 tcaacacatt gatagcattt attatctatt atccatttgt aaaagcttat gataaaaaga
     7321 tgctgtcaga tgaacaaagt cgtggagaaa acgaagtcca gactgtctaa gtttggtttt
     7381 tagaaggtgg gaaacaatgt attggctgat tttatttttt gtttttatct ttttattaac
     7441 ggctagtcac cttatattga atatgctagc tacatatcat attcaaatca atcgatggat
     7501 ttgggcactg gcatcatttt tgatcgtgat tcttccaaag atcattgtac cgcatatgaa
     7561 tgttttgttt tcatggggga catatgtact ttgtggaatt tttgctatca attttatgat
     7621 cgagcagcat agatggtttg tcacatcgaa gctttaaatg ttctaaaagt ggttgtaaca
     7681 gaagtggtta gcttcaagaa ataagaagaa atttacgaaa attgcttctt aaatttttgt
     7741 gaaatgttgg cttatttttc gtttggttga acttattacc gcagatgcaa gcttttgacc
     7801 accgtttatt cggaatatgg gactgtgaca aggcttttgt cacagccctt ttttacataa
     7861 agattattat tgttttaact gttttggcag aaagaaggag gaaaaaatgg aaattttatc
     7921 agcaatggat ttagaattag tagatctact accaaagtga tcaagtcaaa tatagatttg
     7981 ggagcttaca accatatagt aggggctgca gtatggtgta tgaatggtaa tatgtatgat
     8041 gtgagtgttt attcaaatca tgaaacgtgt atagaaataa ttgtgctggg aatagcaatc
     8101 agtcatggtg aacgtgactt ttagtgtatc gttgctgacg acatagaaca ttctgacatg
     8161 atctactcaa tatgtgggga ttgctggcag tttatatagg agtatgcatc ggattgcgag
     8221 atcattgtct caacagaaga gggaggttga ttatcaaaga taaagtggta aatcctacat
     8281 ttgatttagt aattgttgag gggactagcg gcacaaatct ctacaaagcg atgcttctag
     8341 atctatatga cacattttaa gaaaatgtat ttgcctaatt attatggtct ttcttttgaa
     8401 gaaacgttga gatgatccaa ccagagaaag gtgtcaaaga atttgactaa gaataaatgg
     8461 cagacgctac ctatgctcat ttggatagcg tcagatcttt tattttaagg atgattttgt
     8521 gttttttaga caccgtaatt taagtaactc taacttttca atccactatt tacttttttt
     8581 gtattttcac tgtctgggtc tcgaatagcg aaattactga tgagtctcta ttaaaaaagc
     8641 tttttctact tgaaactata ttgggtggtt gctcaggaag gtttcgattt cttgaattgc
     8701 ttgttgtgga ccatttattg gaaaaacggc ttgcagcgtg cgtgcctgct gacgaatctt
     8761 gggtgaagaa acttcgcgca gagcaatctg cagttttctt tcagtcactg gttttgtgag
     8821 ggcgattgcg tttccgtatt ttacacaata gtcaatatta cagctttgct caaattgcat
     8881 ccccacaccg ataaacggtt tgccggattc acaggcagtt tggacggtcc cttccccgcc
     8941 gtggataaaa gaaaaatcaa tgaattcatg caaaagatga ctagacagat attctgttat
     9001 taaaagatct tctgagtcag attgaatgtc atcaagcaga tagtcaatag gtgcaataac
     9061 cgctagatcc ggttgttttt gtaagaagaa caaaattttg cgtaacatac gcggattgcc
     9121 tgaactaccc attgcgaaat agataatacg ttttctttct tgttgctttc gctgtataaa
     9181 agttagcact tcttcaggca aggaagtact gagcttagca aaaattggac caacgattcg
     9241 ataattttcc ggcagctgtt cttcttccaa aaatagttta ggcgttgtga tgagatttag
     9301 atcagcatcc atcatttgta aacttgtttt tggtaaagga agcatttctt tttgtgcagc
     9361 gtgtttgaag gcattgggga tccatcgggt atttactata cgcttttttg tccagtgcca
     9421 aaggggattg cttaaagggc cgaagaaacg taactcgtgg ggcatcagcg catgctctga
     9481 ttgaaaaaaa ggttcactca aagcataggg tttaacatat actaaaggaa tctttttatt
     9541 gcgggccgat aaaaaaattg tcacattcga acctgtcaca atcagtcgag gctcaaactt
     9601 agcaatcaga ctctgttcac tagtgatacg agaggcaacc atttctttgg taaaagggtt
     9661 tttaagtgta cgcaactggt caaattttat gatttgttct tcttgttttt tcgtaaggat
     9721 cggcgctagt aaatgatact caaagccact ttctgtgatc aaaggagcaa aagttttaga
     9781 aaagccaaag aatacacagt aataacggtc tttcatagct ttggcaacct caatcattcg
     9841 tgttgtctca gctaaattga atactgcagg agcaaaaaga attgtcttca tgaaatcctc
     9901 cttattggaa tgagtctatc ccttcgtttc ttactagtat actaaaaaac acagcgttcg
     9961 agtagcctat gtatattttg aatattaaac tgtctctcgc tgttttcaca acttctaaat
    10021 ttaaattttg taatcaagtt gaaactccaa ttttcaaaaa aacacgcaac aattttttga
    10081 tcgatttata tgttgtgcca gataaatacg agaagttgtg tcattaaaaa taaacatgag
    10141 aacataaaaa aataaaataa ttatattaat tgataggtgt gctgtcgtca ttatacacac
    10201 gtaagaaaga aatagttatg tttaaaaata ttcttagact ttctcg
//
LOCUS       DAYYNG010000099        10135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000099 DAYYNG010000000
VERSION     DAYYNG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.099"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..478
                     /locus_tag="ACHBFA_001986"
     CDS             5..478
                     /locus_tag="ACHBFA_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM9395430.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            487..714
                     /locus_tag="ACHBFA_001987"
     CDS             487..714
                     /locus_tag="ACHBFA_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395431.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1350..1721
                     /locus_tag="ACHBFA_001988"
     CDS             1350..1721
                     /locus_tag="ACHBFA_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395432.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1977..2219)
                     /locus_tag="ACHBFA_001989"
     CDS             complement(1977..2219)
                     /locus_tag="ACHBFA_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9395433.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2251..3153)
                     /locus_tag="ACHBFA_001990"
     CDS             complement(2251..3153)
                     /locus_tag="ACHBFA_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9395434.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3166..3354)
                     /locus_tag="ACHBFA_001991"
     CDS             complement(3166..3354)
                     /locus_tag="ACHBFA_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9395435.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3368..3931)
                     /gene="amaP"
                     /locus_tag="ACHBFA_001992"
     CDS             complement(3368..3931)
                     /gene="amaP"
                     /locus_tag="ACHBFA_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9395436.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3969..4862)
                     /locus_tag="ACHBFA_001993"
     CDS             complement(3969..4862)
                     /locus_tag="ACHBFA_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9395437.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4940..5863)
                     /locus_tag="ACHBFA_001994"
     CDS             complement(4940..5863)
                     /locus_tag="ACHBFA_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9395438.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5912..6262)
                     /locus_tag="ACHBFA_001995"
     CDS             complement(5912..6262)
                     /locus_tag="ACHBFA_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9395439.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6295..7197)
                     /locus_tag="ACHBFA_001996"
     CDS             complement(6295..7197)
                     /locus_tag="ACHBFA_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9395440.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7190..8047)
                     /locus_tag="ACHBFA_001997"
     CDS             complement(7190..8047)
                     /locus_tag="ACHBFA_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9395441.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8381..9190
                     /locus_tag="ACHBFA_001998"
     CDS             8381..9190
                     /locus_tag="ACHBFA_001998"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9395442.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9230..9727
                     /locus_tag="ACHBFA_001999"
     CDS             9230..9727
                     /locus_tag="ACHBFA_001999"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM9395443.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         2924 a         1922 c         1743 g         3546 t
ORIGIN      
        1 aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt
       61 tattttgttc ggcgtacttt tttatactca gatcgctaca agcgaaaaag gattcaattt
      121 tgattggcag accacattat ttgtttctgt catttacttt gcccttctcc tagggatcgt
      181 tattgcttat ttcttattga aactattgaa aaaaatcgaa gataaccaag ctttttccac
      241 tcaaagctta aacatactaa aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt
      301 ttttggctta cttcctaaat ttatgaaagt agcggattta gacgatgctc caggtttgat
      361 gttggtagga atcgctgtcg tattcattcc ttttgcagta tacacattaa caagcgtgct
      421 ggaaaaactg cttgaacatg cgattttact taagacagat catgatttga cagtgtgagg
      481 tgcgacatgg caattatcgt taatttagat gtagtattag ctcaaaaaaa aatgagtgtg
      541 actgaattga gtgagaaagt gggcattacg atggcaaaca tctctattct aaaaaatgga
      601 aaagcaaaag ccatccgttt ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa
      661 ccaggtgatt tgttgaccta taaaaatgac tcgaatacta taccagaaga ataaaataaa
      721 gtagaacaaa cagtaaataa aaaaaaatct gaatgataga tgtgtgagac ttttagaagt
      781 tatttacgtc tattattcag attttttatt tcgattgaat accgttttat ctataaataa
      841 gatatgtact gactcataca aagggggcct tttcttctcc atgtactatc acttttcctg
      901 atacaactgg tttcctgtta cgttgtagtt ggatctgctt ttagtataat actttattca
      961 ttccaccaga ctgatagcag tttgagcagt ctaaataatc atgtagtatt acaggccatt
     1021 ctgtttcata ccttgaagtg cttaaaactt cagctaatgg cccattgtgt taactcttac
     1081 tccattcttt tttagtaagc tcccatatat actgtaaagc cttctatata ttatgtcatg
     1141 ttgcttttta acatttacta tgtagttttg ataaaatgat ttgaataaca gtgatggaca
     1201 attctttttt atttttaaca ttattttgat aataatgatt atttatttaa aaatacatta
     1261 gataacattt gttacgacac gaaaaagctt cacaaacaaa tatcgttatt ttaaaatgag
     1321 ggcgaactca cacaattagg aggaatagta tgccatttat tgtttacttt tttctttcat
     1381 tactcgtgat ctatattcca ttaccaacat ttttcctgct cgtcaatcgt ctagcagagc
     1441 aacatgatac gattgctgct cttacgaaac tttgtttact tctatttgtt tgtctagatt
     1501 actttacgag ctattattgg ctaaaacaga caagagtaaa aaagagatat tatttcttcc
     1561 ttgtcttttt caatctcttt gagtttctga tccattgctt tttacttcat cagtatcata
     1621 cgatccattt gtggatcatc tgggttttca aagtggtcgt attattttgt atgttcacct
     1681 ttcctatctc aagaaaattt agggaggcat tgttttatta aagaagcttc tctgtatcaa
     1741 taaagacaca tttaaggaaa aacttttaaa aatctaattg ggaatttgcc cgcataaata
     1801 agaggctgtg acaaaagtct tgtcacagcc tcttattccg tataaacagt gttcaaaagc
     1861 ttgcatcttc gataatatgc ccaaccaaat gaaaaatatg agaagctatt tttgtttggc
     1921 tgttcttatt acttgatgaa gaacagcttt ttcacgacct ctttttaaaa gtctttttac
     1981 agttttcgag caaagaatga tacgattagt actaaaatga tggcaccaat caatgatggc
     2041 acgattgcca ttcctgcaat acttggtccc caagaaccta ataatgcttg accaagtgct
     2101 gatccaacta aacctgctac gacatttgca ataatcccca tcgaagaacc tttattcgtg
     2161 attgctcctg caattgctcc gataattcct ccgacgataa gtgaccaaat aaatcccatg
     2221 tttcgttcct cctaatgttt tacttcctta ttactgaacc cgtggttctg tattttcttt
     2281 tactttttgt gcgccgcttc cagctgctga ttttacttta tctgtttgat tcgaagcaaa
     2341 ttcaccggtt tttttcgctg cgtcagttac tttgtcttgc accgtttctt gatctttttc
     2401 aaattcttct tttgttttga tgtccactac gttagcgttg acttcgatca cttctaaatg
     2461 tgtcatattt tttacttctt tgccgattac ttcttgcatt tcttcaaaga ttttttcgat
     2521 atctttgcca tattccacaa tgacatccat atcaactgct acttgttttt taccgacttc
     2581 tgtttcgatc ccagccgtta cattatctgt attcacaagt tttcctgcca cattagagaa
     2641 gaaaccaccg tttgcagata ataaaccgtc tacttgttca attgcgattc cgatgatttt
     2701 ttgcacaact ttatcttcat aattcagctc accgcgcatt gaatcttcct gttcagaatc
     2761 atgctttcct tggttatttg tatgatcaga accaagtttt ccttgtccgt ttgtgtggtc
     2821 agaactaagt ttttcttgtg ctttcgcatg gtcagaagga tgtttttgct cttccacagg
     2881 tttagaagga tcgattgcat catgttgttc tggaacttta gattctggag ttcctggatg
     2941 atgaggtgca tgcttttctg catcatgttt cttctcttct actccttgtt gaacgttagg
     3001 gactactgga tctggatgtg cagatactgg tgctgttttg ttcttttcat cgttcacacc
     3061 atttgattgg tttggtgtcg ttgggtctgg atgtgcagac actggttttt ggttgttcat
     3121 tttattgtta ttatttttgt tattgttatt catggatgtt tcctcctaag atttaaaata
     3181 gtgatctaag attttcgttt gtttcagata aaaaccgata gcgacgccga tcgctgccat
     3241 gatcaagatc aataaggtct tgaaaaagcc cacggtgatc cataaaacgg ctaatatcaa
     3301 tccaagtacc ccaaagatga tagggagctt gtatgtcgaa aatagttctt tcatttttgg
     3361 tcccccccta ttctactcgt ttgcggtcat tgcttcgttt agctggttct tcaaagcctg
     3421 tgtatttcac cgatactttc actggctctt ttgttcccaa tatgtgttgt acttcatttt
     3481 cgatttcttt catcaatgtt cctgttttac caatgagtgc tgaagttcgt ttcaactccc
     3541 cttttacatt gacggcgatc ttgtttttcg ttgctttgac atttactttt ggtgaatgga
     3601 tgaattcatc ttcatgcaag tgagaacgaa ccattccttc aattgctttt ttatccaacg
     3661 tcaactcccc atcaccatgt ttcagtagaa agtttccgat tgtttttgga taaaacagga
     3721 aaacaagtaa cgtgataatt gtcaaaatag caaatgcaac ggcaagccaa aatacatatt
     3781 gttgcacgta tgaattagtg atggaaaaga atcgaaactt ctctagctga aaaggcaatg
     3841 gataggttgc acttaatagt atcgcaaaca aaagcacaga tagcaaaagt aatgttccga
     3901 tcactccgat cgtttttaca cctttattca ttttcgcacc tcctcatagg ggtgaaacga
     3961 tcaacttctt ataagttgat cgcttctcca ccagttactc cgtaaatttg ggctgtaaca
     4021 taacttgctt tattcgatgc taagaaaaca taaactggtg ctaattctcc tggctgacct
     4081 gcacgttcta gtaaactttg ctgaccaaat tcagggatcg aatcagaagg ttgtccttcg
     4141 tctagttgta atggtgtcca gattggacca ggtgctacac catttacacg gattccttta
     4201 ggtgcaagct gttttgctaa accgatcgta aagttagcga ttgaagcttt tgttgctgcg
     4261 taatctaata aatgttcgct tggattgaat gcttgaacag aagttgttgt tacgattgct
     4321 cctcctgctg ggatatgcgg aacagcttct ttcactaaag caaacatagc gatgatgttt
     4381 atcataaatg tgtctttgac ttgctcaagt ggtaactcag caatagactg acaagaaatt
     4441 tgctgtcctg cgtttagtac catcgtatct agcccgccaa gttcactcac ggctttatct
     4501 atgatttctt taggtgcttg ttcgtcacga agatcaaaag gtagtaagac aatcttacgt
     4561 cctgcatctt tcacgtattt tgctacttcc tctgcatctt tttcttctcc tgggaagaat
     4621 tgaagagcga tatctgcccc ttctctagca aatgcaatcg ctgctgcacg accgatccct
     4681 gaatctccac cagtgattag tacacggcgg ttttctagtt gattattgcc ttcataggat
     4741 tcttctccac aatcaggaac aggaatcata ttctgctgta aagctggtgt atcctgatct
     4801 tgtttaggaa atttctccgt atggtacaag tttcgcgggt cgattaattt tggttctgtc
     4861 attgctgtat tcctcctagt ttttaaattg tattgccaaa acaaatttct ttgtttgata
     4921 cctgttttaa atagattctt taatttgtat aatctttccg gtataaatgg taacctaaaa
     4981 ctactgctgc tatcaaagag ccaaagacta cgagtataaa gcctagagca gaacttttac
     5041 ccggtaatcc tcctgtattc attccccaaa tccctgcgaa caacgccgga acggaaatga
     5101 ttaatgcagc tgaatctaga tatttcatga tatgatttag attattgtcc atcatcccgt
     5161 tgaatagatc accaatactc tctaataaat ctcgatagat ctcgatcatt ttttctgttt
     5221 gtcgctgtct caaatggaca tcatagagta gctgttcatc gtctaaacgt ttgatgaaag
     5281 tttcagaatt ccataattga tccattgttt cccgttgatc tcttaaggta tgatcgatat
     5341 atacgatgtc tctttcaaga tctgcctgtt tatacagttc ttcattttcc gttgttttac
     5401 gagctgcttt atccaaagcg tcaattcgct ttttcatctc tcttagttct ttcacgtaat
     5461 gggtgtaaat atctaataat gtatacgtga tgatcttttc aaacgagttt aaatgatttc
     5521 catgctttgt cagcagccga tccacaaatt cggtttttct atcgatacaa gtaatcaaga
     5581 gattatcgga aagtacaaaa gatattggcg ttaatttttt ttcgatacga ttatctgact
     5641 gatctagatt aatcactacc aaagaaaatg gatgctctag tttcgtctca taaaggcgct
     5701 ccatccgaga aacttcttcc ggatattcgg ttcctacgaa aatatcatca ggaagctcga
     5761 attcttcgat tgcttgctca atctcttcgg gctttgcact atcaagtacg agccaattat
     5821 attttttttc atctgtttct attacattct tttcttcatc cacaaagtag tatctcaatg
     5881 tatacctcct ttttggactt tctcgaaact attagctgaa gaagcagttc tttaatttga
     5941 tattataatc gagtactgcg atttcatttt ctaatgaaaa gctgtcaagc tgtttgttga
     6001 agtccatgtt caatcgttta ccgaaataat tttgctctaa tttagatgta tagatgtcca
     6061 ataatggatg ctcgatttta gtcgcatatt ctccaaattc cgaaacaaaa ggaatgattt
     6121 gaaaaaagct gccttttgca aaagcacctt tgtcccttag aaatggatcg gaatcattga
     6181 taagcgcgag tgcgattttt gtattttccc caaccttttc aattaacgtc tctactgcat
     6241 ttttagacat tgtaatttcc attcctactc cccctcttca gttgttgtct tctattattt
     6301 aacaactaca tctgtttttg gttgaacttt tgcatatttt ggatatttag ggaatttagc
     6361 tacacaacgg aaagccgtca tccctgcata tttttcgtaa tatttcttcg ttggcccttc
     6421 aaaatccttg ctccacggtt tcacatgtcc tgaaaaatgg ataatgtaag gattgttgcg
     6481 agtttcttcg tattcacgtt cacctgctgc tgttggatgt ttttttgctt ttgccatgat
     6541 atagccttgt gcattccaac gcggatgaag tggtaaccaa cggtcatgta agatagcatt
     6601 taatgcatct tggtcatgga atcgtaattt ttctggatta tgttcgatga aatctaatac
     6661 tttttgcgta atattttcat ctaaccattt tttcacattg atcagcatca aacctgagtt
     6721 gaaataacgc attgatttag ctgaaatttt cattttttcc agtcgttgat ggaacccagc
     6781 gtcttcaaca gctgctacga ttgaatcacc gaagtcaaga cgccataatt tagagatatc
     6841 ttgtaaagcg atcatgtcgc aatccatata taagatacgt tcaacttctg tgccgcggaa
     6901 caattccgga atcgcaatac gataataagc tgtttctgga atacgatcgc tgattaccac
     6961 atttgtaaag aattctttat tgattttcaa gaattccaca tctgagttca ttcgtgcgtt
     7021 tttcactgag aagcgtaaaa gctgtttact ttcaaaatcg atatcatcgt caatcacata
     7081 gaaccgaaca aatttagacg ggttgcaatt atctaatact gagacaaata atgcagctaa
     7141 atgtggtacg aattttgtgt tacaacttgc gactactgct atttcttttt tattcattga
     7201 tctcgcctac tttcgttaga gtactgtgtg ctaatttttt taagtataaa ttggtatacg
     7261 gatgatcttt caacgtattc caaggtttat catgtcctgt aaagtgaacg atcgaaggtt
     7321 tttcatttcc ttctttatac tgacgttcat atttttcatt tggtgctgga tgacgttcaa
     7381 aaatcagcga agtctgcatg ttccattttg gatgaagttg ttcccaatct tcataaagta
     7441 cagcattcaa agcatcttgg tcgtgataga tgattcgatc gccattttca ctgaggtaat
     7501 ggatagtttt ttctgtaatt tcttttttat tccattgatc aatgtcaatc accatcactc
     7561 ctgaattgaa gtacaagtca tcggaatcga ttcctaatct ttccaaagct ttcacttggc
     7621 ctggatcgat aatagcgccg attgtttttc catttaacgg ctcatcatat agttttacga
     7681 tatcgtctag tactaacaca tctgaatcta agtaaaggac tttcttataa tcctctttag
     7741 ctaataagtt tggtaaagaa attcttaagt aagcggtagt tgtgatatgg tcactgacta
     7801 ggaagtcttc gtagatatct ttttccactg tcaggaattg gatacttgca ttacttgaat
     7861 atttatttac cgtttcttcc aatccttctt tactatattc tgatagacca tcgtcaatca
     7921 cataaaattt tatgcgtctt gttttattgc aattttctaa tgcagttgcg atcatcacgc
     7981 tcaagtaagg agcataattt tcgtctgaag cagttaccac aggaactacg ccgtatctct
     8041 tctccatcaa aatcactcct ttttctaatc tttttctaac ctgttctaca tctagaatat
     8101 tcctcatttg gttttaaagc aaaatatatg ctttgttttc attgaatatt ttaaaatttt
     8161 tttatcacaa aacgcatcgg taagcgctaa ctattcgttt ttcatggctt tttattcaaa
     8221 ggtattgacc aaaaactact atatatagcc acctttttct taagaatgtt ttttgaaaaa
     8281 attcataatg acaaagcaag ttagtttcat ttttttaaat tctcatttaa tcttaaggta
     8341 acaggaaaat agatatgtaa aaagaaagta ggaagataaa atgaaactag cagcgattga
     8401 tttagacggt accttgcttg atcgaaatgg aaagatacca gaagaaaata tccaagcact
     8461 tcggaaattt gatgaaaatg gcggaatcgt aaccgtcgct accggaagga acagcatttc
     8521 tgctaaagat attttttccg aattaggaat cggtggctat ttgatttctt ccaatggttc
     8581 actgattgct gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga
     8641 tagcgataca ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc
     8701 tagccgagaa acccaagatg atcagctgac attcgatgaa tcaaatctag tcaaagatga
     8761 cccttattat gaacatttca atttacagaa ccattctttt gatcaaattt taaatatgtt
     8821 ggaagatcct tctcttagct atcttaaatt agcattaaca gataagagtg aagaaaaact
     8881 acagaagcta caaagagatc tgcaagcaga tggtatccaa tcagtattct ctgatccgca
     8941 tttcttagaa atcacgccta caggtatcac taaagctcat tcacttgttt ttctggcgga
     9001 ttaccttgga atcgaatcga gtgacatcat ggcttttggc gatcaagaaa acgacattgc
     9061 tatgttgaat tacagcgggt tgagtattgc gatggggaat gcacagcgac aagtaaaaga
     9121 aattgcaaac caagtaacac tgacgaatga agaagctggt gttgcttata ttttaaaaaa
     9181 cttctattaa aaagaataaa atgacgatat gaatgtagga ggaaagaaaa tgttagttat
     9241 tattgatatg caaaatcata ttcttgatcc aaacagcgaa ttttatattc cagagtcaga
     9301 tcaattggca gaacgtattg ctcaaagatt agctaaagca cgccaaaata atgaatatat
     9361 tctcttcaca cgggatatac cagttgaact gaaggacaaa gaagaagaac gagaagattt
     9421 gcaattgatc cctcttcttt ctccacaaga aaatgaacga gtgattaaaa agtattactt
     9481 tacgattcca cctgaaactt tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga
     9541 acaaaaagag atcgaagtag tcgggattga aacaaacttg tgtgtactct ctaatctgat
     9601 aggcctgcag agtgcttttc ctgaagcaga cttttttgtt gatccaatgt tagtctccag
     9661 tagaaaacac ggagaatccg ctttagagct gttgaaagat ttcaacgtct ctatcgtcga
     9721 atcataaaaa tatctatctt catttacgat atttaacaca caataaaaaa agcacgcaga
     9781 actgttttag tcctacgtgc tttttctatc aaagtagatt ggctttttta tgacacagag
     9841 tgaaacggca ggctcacgac ctctttctta aatggtgttc aatcagctga ccctcggtat
     9901 tagcttaaca aacggcaaaa tatgaggaac tatttttccg tttgttaagc taatactcag
     9961 gtcaaaacgc tgatttcaca acctcttcct taaacggtat tcaaaagctt gcatctgcag
    10021 taataagaac agccaagtaa aaacataaga agctgttttt acttggctgt tcttattact
    10081 tgatgcaggg cagctttttc acaacctctt atgtaccttg taggactcga accta
//
LOCUS       DAYYNG010000100        10102 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000100 DAYYNG010000000
VERSION     DAYYNG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.100"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..341)
                     /locus_tag="ACHBFA_002000"
     CDS             complement(21..341)
                     /locus_tag="ACHBFA_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9395444.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(489..2723)
                     /locus_tag="ACHBFA_002001"
     CDS             complement(489..2723)
                     /locus_tag="ACHBFA_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9395445.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3202..3468
                     /locus_tag="ACHBFA_002002"
     CDS             3202..3468
                     /locus_tag="ACHBFA_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9395446.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3468..5195
                     /gene="ptsP"
                     /locus_tag="ACHBFA_002003"
     CDS             3468..5195
                     /gene="ptsP"
                     /locus_tag="ACHBFA_002003"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9395447.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            5359..6405
                     /locus_tag="ACHBFA_002004"
     CDS             5359..6405
                     /locus_tag="ACHBFA_002004"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9395448.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6422..7645
                     /locus_tag="ACHBFA_002005"
     CDS             6422..7645
                     /locus_tag="ACHBFA_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9395449.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7734..8621
                     /locus_tag="ACHBFA_002006"
     CDS             7734..8621
                     /locus_tag="ACHBFA_002006"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9395450.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELTDKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8707..8937
                     /locus_tag="ACHBFA_002007"
     CDS             8707..8937
                     /locus_tag="ACHBFA_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM9395451.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(8994..9959)
                     /locus_tag="ACHBFA_002008"
     CDS             complement(8994..9959)
                     /locus_tag="ACHBFA_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9395452.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3225 a         1779 c         2041 g         3057 t
ORIGIN      
        1 ttattatgtt caaaatttgt ttacacggct aatgtagcgc ctaattcatg ttttggaatc
       61 gagatttcaa caaggcctgc caattcgatt actttgattt catcctcgtg gtaaacggcg
      121 tctttgattt tatctaagaa ataattttta gcatcttcga tttctttcgg ctggatttta
      181 cgtttgtcat tgatcagcag aatttcatta tgttcagggg cttcagtgta gacaatagtg
      241 gtatttccgg ctgtgtagac cttgactaga tgggcatcag tgttttcaag ctggtttaat
      301 acaagacggg aatggctgtt tgttacattt accaatttca tggcgctcac ctcttttatg
      361 tatttttaat tgcttgtttc tcttattata atttcttttt tataaaatga acagtatttt
      421 gacttttctg aaaaaagaat gagacggcat ttctcaaaga aataccgtct cacgctataa
      481 aactggattt attcagccgt ttcttcggct gattctttag ccaaacgttc tggtttagaa
      541 gtaacgatga tttttccatc tttatccagt tttgctttca actcatggat agaagggtga
      601 tcaagataaa attcagcgat accatcttcg atttgttctt ggatcgtgcg tcgaagagga
      661 cgagcaccca tagatggatc atagcctaag tcaactaatt tttctttgac attcgtagga
      721 acttcgatgt gaagttgctg tgctgccagc atttggttga catcgtctag cattagtgtg
      781 acgattgtca tcaagttttc ttttgataat gcactgaatt cgatgatccc gtcaaaacgg
      841 ttcagaaatt ctggtgtaaa gtagttgttt aattggttca atacagaacg agtaacacct
      901 tcgcgtgccg caccaaaacc aacgtttgct tctactttac cagttccggc attacttgtc
      961 ataatgatga tcgtatcttt aaagctgacc gttcttcctt gtgcatctgt caatcgacca
     1021 tcatctaaga tttgcaagaa catatgcaaa acatctggat gagctttttc tacttcatct
     1081 aacaagacaa gactgtaagg atttctccgt actttttctg ttaattgacc agcttcttcg
     1141 tatccgacat aacctggagg agaaccaatc aatttagaga cactatgttt ttccatatat
     1201 tcagacatat cgaaacgaat cattgaatct tgtgaaccga atagttcgta ggcaagttgt
     1261 ttcgcaagct ctgttttacc tacaccagtt ggtccaacaa ataagaagga gccgattgga
     1321 cgattctgtt tgtttaatcc aacacggttt cggcgaatcg ctttcgctac tcgatctaca
     1381 gcgttatctt gtccgatgac atgagctttc aagtcatctg ctaagttttt cagttgtgtc
     1441 tgttcttttt cttttaattc acccactgga atacctgtcc gttgttcaac gattttttcc
     1501 atatcttttt cggtaatcac aggtgtttcc tcttcagtga gctgacgttc tttcattttt
     1561 tgaagtttat tgatctgatc gcgataataa gctgcttttt cgaaatcttc ttctcgagaa
     1621 gctaaaactt tttgttcttc tgcttcttgc agttttttct cgattgtttt aggatcaacg
     1681 atttggatcg tcaagttttt cttagaacct gtttcatcta gcaaatcgat ggctttatcc
     1741 ggcaagaagc gatcttggac gtaacgattg gataaagttg cggctgcttc gatggcctca
     1801 tcagtatatt ttacatgatg atagtcttca taacgttttt gcaaaccttt caaaatactg
     1861 atcgtttctt cgacagttgg ttcatctacc cgtacaggtt gcatacgacg ttctaaagca
     1921 gcgtcttttt cgatgatgcg gtactcattt aaggtagtag caccaaccat ttgcaattct
     1981 cctcgagcta aagctggttt taaaatatta ccagcatcca tatttccatc accagcagaa
     2041 ccagcaccga cgatttcgtg aacttcatct atgaatagga tcacattttc tgcttgacgg
     2101 atctcttcaa tcaatttttg catacgttct tcaaattgtc ctcgtatacc agtaccttgg
     2161 actaaagata cgacatctaa acgaatgact tctttgtcca ttaatttttg cggtacatca
     2221 ccatctacga ttttttgtgc taaaccttca acaactgctg ttttaccaac acctggttct
     2281 ccaatcaaga caggattgtt cttggttcgg cgatttaaaa tttcaatgac tcgtttgatt
     2341 tcgtcatcgc gtccgatcac aggatcgata tcaccttgtc gtgcttgttc tgtgatattg
     2401 attccgtatt ctcccaataa accttctgta ttgccttgag gtggctgacc accattgaag
     2461 ttgttgtttc cgccaaattg tgttggtggt gtttgctcat aatttccttg ctgttgcatt
     2521 tggcgagaca tggaacggaa aagatcgtcc aaactgccaa atccaaatgg atcattttgg
     2581 gccatattag tcagacctcc attttgttga tttttgattt tttgataaca gctctgacag
     2641 taatcgagtt gtgtccgctg accattgaca gtggcataca agtgaatcgt tgcctcattc
     2701 ttgccgcagt tttgacaaag catgaatcat tcacgtcctt tcacaaaaat gggtacatcc
     2761 atattttaca tctcgttttt catgaggtaa aagaataacc cctagttttt taagagattt
     2821 atgaggtaga aaaggtgaaa tctctattga ccattcctga cctttgactc ttattatact
     2881 aaaaataaaa agggaagcaa gtaattcgtt tcatatgaaa acaaagaggg tcaaaggaaa
     2941 gaatgataaa cgtcaggaga agagaaagcg acaagaaaaa ggaaataaac acgtttgtta
     3001 taaaatgtga agaaaaggat gaaaaagaat ccggaataat ttgaatactt ttatctgaaa
     3061 ttgaatgaaa actgttgtat ctaacgaaaa aaaatgttaa tctttacaga tgaaagggtg
     3121 gcatttgtta ggattctctg aattactaaa aaagaaaacc ctcacataaa aacaacactc
     3181 acttaaagga gaccgattaa tatggaaaag aaagaatttc acgtagtagc agaaactggg
     3241 atccacgctc gtccagctac actattagta caaacagcaa gcaaatttaa ctctgatgta
     3301 aacttagagt ataaaggtaa atcagtaaac ttgaaatcaa tcatgggcgt tatgtcttta
     3361 ggtgtaggtc aaggttctga tgttactatt actgcagaag gtgctgacga agctgatgca
     3421 atggcagcaa tcgttgaaac aatgaagaaa gaaggcttat ctgaataatg gttgaaatgc
     3481 taaaagggat tgccgctagt gacggtgtag ccgttgcaaa agcttacctg ctagttcaac
     3541 cggatttaac attcagtaag gcaacagtag aagatactgc ggctgaagaa gctcgtttgg
     3601 atgctgcatt agctaaatct actgaagaat tgcagcaaat tcgtgaaaaa gcagcgcaaa
     3661 gcttaggtga agcagaagcg caagtatttg atgcccattt gatggttctt tcagatccag
     3721 aaatggtagg tcaaatcaag caaaatatta aagacaacag cgtaaatgct gaatcagcgc
     3781 taaaagaagt aacggatatg tatatcggta tgttcgaagc aatggaagat aatgcttata
     3841 tgcaagagcg tgctgcagat attcgtgacg ttgctaaacg tattttagcg cacttattag
     3901 gcgttacttt accaaatcca tcaatgatca acgaagaagt ggtcgttgtt gcacatgact
     3961 tgactccaag tgatacagca caattagatc gcaattttgt aaaagcgttt gtaacagaca
     4021 ttggtggacg tacttcacat tctgctatca tggcacgttc tttagaaatt ccagcaatcg
     4081 ttgggacaaa agaaattact gctaaagtca aagaaggcga tattttggct gtcaacggga
     4141 tcgaaggcga tgtaatcatc gatcctacag atgaacaaaa agctgaattt gaaaaagcag
     4201 gtgcagatta cgcagcacaa aaagcagaat gggaaaaatt aaaaaatgca gaaactgtca
     4261 cagctgacgg caagcatttt gaattagctg caaacatcgg aacaccaaaa gatctagttg
     4321 gtgtccataa taatggtgga gaagcagttg gtctgtaccg tactgaattc ctgtatatgg
     4381 attctccaga cttcccaaca gaagatgacc aatatgaagc ttacaaagct gtattagaag
     4441 gtatggaagg aaaacctgta gttgttcgta caatggacat cggtggagat aaagaactac
     4501 catatctgca attacctcat gaaatgaatc cattcttagg atatcgtgca ttgcgtatca
     4561 gtttatctga acaaggcgac gaaatgttcc gtacacaaat gcgtgcattg cttcgtgcat
     4621 ctgttcatgg taatttacgt atcatgttcc ctatggtagc tacactcaaa gaattccgcg
     4681 cagcaaaagc gatctttgaa gaagaaaaac aaaaattgat aagtgaaggc aaggaagtat
     4741 ctgatacgat ccaagtcggc atcatgatcg agattccagc agcagcagtc ttagcagaca
     4801 agtttgcaaa agaagtagat ttcttctctg taggtacgaa cgatttgatt caatacacaa
     4861 tggcagcaga ccgtatgaac gaacgcgttt cttacttgta ccaaccatat aacccatcaa
     4921 ttttacgttt gatcaaaaat gtaattgatg cagcacacgc tgaaggaaaa tgggcaggta
     4981 tgtgtggaga aatggctgga gatcaaacag ctgttccatt gctagtaggt atgggtctag
     5041 atgagttctc aatgagtgcg acatctattc ttaaaacgcg tagtttaatg aaacgcttgg
     5101 acacagcgaa aatggctgaa cttgctgatc gtgcattgaa agaatgcgat atgatggaag
     5161 aagtcgttga acttgttcat gaatatgtaa aataaacaac gtatcaaaag ctggtgtggc
     5221 ttgtaaaaga gctacgccag tttttttgtt cttttttaag aaaacgtttt tgtcagtaag
     5281 aatagacaaa aatagggctt aagtttgagt gacagtttgt aggctatgtt atactatata
     5341 agtgaagggg gacgtaaggt gaaggtatta ttatattttg aaagtgaaaa gatgcttgca
     5401 aaatctggaa tcgggcgtgc gcttgatcat caaaaacgtg ctctgacgga agtcggtatc
     5461 tcttatacac tagatgaaaa agaagattat gacatcctgc acatcaatac atacggaatc
     5521 aatagtcata atatggtcaa taaagccaga agagaaggaa aaaaagttgt ctatcatgcg
     5581 cattctacag aggaagattt ccgaaattcc tttatcggat cgaaccagct ttctcctatc
     5641 gtaaaaaaat atctggtagg attgtaccaa aaagcggact atctgatcac gccaacacct
     5701 tattcaaaac agttgttgga aagctatggt atccgtgtgc cgattcaagc tatttccaat
     5761 gggatcgact tagagaaata tcaccctgat ccaatgaaag aacaaaagtt cagagaatat
     5821 ttcaaactgt ctccggatca aaaagtaatc atttgtgtcg gtttgttttt cgaacgtaaa
     5881 ggaatcattg atttcgtaga aattgctaag aagatgcccg aatacacatt tatttggttt
     5941 ggtcatgttc ccatgtactc gattcctcgg aacatccgga aaattgtaaa agaagatcat
     6001 cctgaaaatg tcctgtttcc tggttacatt cgtggagaga tcatagaagg tgcgtattca
     6061 ggtgcagatc tattcttttt cccttcttat gaagagacag aagggatcgt tgttttagaa
     6121 gctttagcaa gcaaacaaaa cgtacttgtt cgagatattc cagtgtataa tggctggtta
     6181 gaagatagca agaactgcta tatggggaag acaaacgaag aatttatccg actgatccaa
     6241 cagattacca atcaagaact accgagtacg acacaagccg gttatcaaac agcaaaagag
     6301 cgaagtatca ataaaatcgg tgaagaactt aaaaatgtat acgaaagtgt actgaatgaa
     6361 aaagtctcta gcaaattaaa aaaagtaaat caggtcaagg actagtttgg aggcgaaaaa
     6421 catgaaaatc ggctttttta ccgataccta ctttccgcaa gtcagcggag ttgctacatc
     6481 gatcaaaaca ttaaaacaag aattagaaag acatgggcat gaagtctata tttttacaac
     6541 gaccgatcct aatgcggata agttggaaaa agatgtgatt cgaatgccaa gtgtaccatt
     6601 tatttctttc aaggatcgaa gagttgttgt tagaggcatg tggtatgctt atttggttgc
     6661 caaagaacta gaactagatc ttattcatac ccatacagaa ttcggagcag ggatacttgg
     6721 gaagatggta gctaagaagt tgaaaatacc tctgatccat acttaccata cgatgtatga
     6781 agactacctt cattatatcg gtaaaggaaa agtgatcagg caatcccatg taaaatactt
     6841 gtctagatta tttgcaaatc atacgacagg tgtagtttgt ccaagtgaac gtgttattga
     6901 tacattacgc agctatggtg taatagcacc gcttcgcgtg attcctactg gtatcgacat
     6961 tgaaaaattc aaacgttctg atatcactca gcaggatatc agcaaattaa gggagtcact
     7021 aggtcttcag gaaaaccatt tgatgattct ttctttaagc aggatttctt atgagaaaaa
     7081 tatccaagcc ttgattaatg ggtttcctca ggtcatctcc gcttatccta acgccagatt
     7141 agttatcgta ggaaaaggac cttatgtaga agagttagag gaattgatct cagaactgca
     7201 gttagaagaa tttgtgcagt tcacaggaga agtggacaac aatgatgtag cactttatta
     7261 taaagcggca gattattttg tcagtgcttc tacttcagaa acacaagggc ttacttatac
     7321 agaagcgatg gcagccggaa caccttgtgt cgtggaagga aatgcttatt tgaaccgatt
     7381 gttcgatcat ccttcattag ggaaaacttt tgaaaccgac gaagactttg caccagcact
     7441 tattgattat atttccagtg aagttcctaa agatccaaaa gttttagaag ctaaacttta
     7501 tgatatttca tctacacatt tcggcgaagc tatgatcgaa ttttatcaag acatggttgt
     7561 ttatcatgaa gaagagatgc gaagaaaaga agaagaagac tcgattgaac gaatcaaaat
     7621 aaaattaaat tcatttaaaa gataaaattc aaacattttt tatgaaaaaa tccttgtttc
     7681 tagcgtatac tagcatagaa gcaaggattt ttgattttta ggaggtcgaa aaaatgaaac
     7741 ttggttttat cggaacaggc gtcatgggaa gtgctgtcgc acgccatcta ctagaagcag
     7801 gacacgaagt agctgtctat aatcggacaa aagcaaaagc tgatccttta gtaacagaag
     7861 gagctatctg ggcagataca ccaaaagcgg tagcagaaca aagtaatatt ctctttacaa
     7921 tggtaggtta ccccaaagat gtagaagaaa tctactatgg acagtcgggg attttttctg
     7981 cagatatctc aggacatatt ttagttgatt tgactacaag tacaccaagc ctggcagaaa
     8041 aaattgcaaa aacagcaaaa gaaaaaggcg cagatgcgtt agatgcaccg gtatccggtg
     8101 gtgacttagg agcaaagaat ggtacactga cgatcatggt cggtggagaa gaagccgttt
     8161 atgatcaagt actgccttta ttcaaagaat ttggtacgac atttacattg catggttctg
     8221 ctgggaaagg tcagcataca aaaatggcta atcagatcat gattgcaggt acgatgacag
     8281 gaatgacgga aatgcttgtc tatgcacaaa aaaacggact tgatttaaag aaagtgattg
     8341 agacgctttc aggtggaagt gcagctaact ggtctttaag caattattct cctcgtattt
     8401 taaaagaaga ttacacacct ggtttttttg tcaaacattt tatcaaagat ttaaagattg
     8461 cattagaaga agcagaaaaa atggatcttg tattgccagc aactacccaa gcattgaaac
     8521 tatatgaaga gttgacagat aaaggttttg agaatgacgg aacccaagct ttgatcaaac
     8581 tttggtggcc tgaaggaaaa ataccagaaa aaaaatcata aaaaacgagt ttttttagag
     8641 aaaatcaatg caagtaggct atgcttttga tacaatataa cagaaataag aaaaggagga
     8701 cctccgatga cacattcaca attagtcgca atcataaaac gactggaggc tatggtcgag
     8761 tcagcagata atgaactgca agttcgccgt tttgaaaaag aaggcgtaga gaagtgtaca
     8821 gtaacttatg ataaagcgac cgatacattt gaattaactg aaacagatac tcatcagcaa
     8881 tacgaatttg ataatatcga tatcgtagcg atggaaatct atgacttaat ccaataaatt
     8941 ttaggataat tgacatttga agttgaagat gcctaaaaga agccgagaag caactaattt
     9001 agttgctttt cagcttcttt tttgtttaac agttcaattt caaactgttc tttttctaat
     9061 tttttgcggt aactgatcaa gatttgtaca ggatcgattt cttcttttga caattgacgt
     9121 ttcaaagata aatgagcttc tttcttttca taatagaagt caggagaaac gtcaccagag
     9181 gtaatcattg tttctgcaag ttgaccataa caaacgaggt agtgcttttt ctgactctca
     9241 tctgctgcta atggcatgct gaaggtgagc gatggtgttc ttaaaaactg cttttgttcc
     9301 tggatccgtc taataaaata agtaactaat tcgatatctt tttgaagcat accggctttt
     9361 tcgaaattca actgatagat gaaatgttgt tgtctttttt tcaagtaagt cagtagcttt
     9421 ttatttcggc cttgaaacag cccttggagt tccttttttt tctgttcaaa agtcatttcc
     9481 tgtaggtcag gaagctggac tcgcaaagct aacaaactaa tttcagacaa ccaaggcatt
     9541 tgataagttt cttccattat acgcaaaata gacggcattt ttttatagag acgaaaaggc
     9601 ccgtatgttc gagccgtagg agtatctgtt aatacaaatc ccttattggt aatatgtaca
     9661 taattataat tactgaaata attcatttgt cggttataaa aaggacggta atgttggatc
     9721 aattgacact cgagcaataa agcatctaat tccgtgtcta ctacgacgaa atcaaaatct
     9781 gttatatgat gaatcatacg taatactttt tttgaatgct gtttattctg atgaaaatag
     9841 ctgcttactc tatttttgag ccgttttgct ttaccaacat agatgacctt accatctcgg
     9901 tctttcataa gatagacacc cggcagcatg gggagagttt tcattttttc ttttaacata
     9961 ggatctcctt ttacgctttg aataaaaaga ctataacaaa ttttgaaata atggacaagt
    10021 tttagggaat tctatagttc tgttcacaga aaatactttg ataatcaaaa tatgtgagat
    10081 agatgatttt tctaataaaa ga
//
LOCUS       DAYYNG010000101        10085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000101 DAYYNG010000000
VERSION     DAYYNG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.101"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..234)
                     /locus_tag="ACHBFA_002009"
     CDS             complement(37..234)
                     /locus_tag="ACHBFA_002009"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9395453.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(380..1258)
                     /locus_tag="ACHBFA_002010"
     CDS             complement(380..1258)
                     /locus_tag="ACHBFA_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9395454.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1297..2415)
                     /locus_tag="ACHBFA_002011"
     CDS             complement(1297..2415)
                     /locus_tag="ACHBFA_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9395455.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2412..4511)
                     /locus_tag="ACHBFA_002012"
     CDS             complement(2412..4511)
                     /locus_tag="ACHBFA_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM9395456.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4562..5383)
                     /locus_tag="ACHBFA_002013"
     CDS             complement(4562..5383)
                     /locus_tag="ACHBFA_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9395457.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5380..6189)
                     /locus_tag="ACHBFA_002014"
     CDS             complement(5380..6189)
                     /locus_tag="ACHBFA_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9395458.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6210..6704)
                     /locus_tag="ACHBFA_002015"
     CDS             complement(6210..6704)
                     /locus_tag="ACHBFA_002015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395459.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6716..7216)
                     /locus_tag="ACHBFA_002016"
     CDS             complement(6716..7216)
                     /locus_tag="ACHBFA_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395460.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7281..9977)
                     /locus_tag="ACHBFA_002017"
     CDS             complement(7281..9977)
                     /locus_tag="ACHBFA_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9395461.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3064 a         2267 c         1660 g         3094 t
ORIGIN      
        1 gaattacgat ttattttata cggctgaaaa aatctatcac caaaaaaatt caaacattaa
       61 gccaaggatt atgaagtcaa catttcgaaa aatgaacata cattggtaag caactataac
      121 tattaccaca gacataaagt aacacaatat attactattt aagtgtacgc caccagtgaa
      181 tatcctcaag gtatacagcg cacaacaaaa agaaaaaagg aaacgcagaa gcatttcctt
      241 tcagtgattt aatgaaaaca acacgatttg acttagagcc tttttatttg aattcatttt
      301 cgctttatcc aacttcattt gatcatttga ttactcggtt aaatactttt gacatgagta
      361 tcaatgaaac attcattaat tatggttgct tatttcttac cgcttttctg gccaatgcta
      421 atccgccgat tattccggca ttatcaccta attcgggcgt aataatgtag ctatcaattg
      481 gtggtatttc aacatatcca tttataagct caacaaattt atcctgaact tttttcttca
      541 tgtgttcttg tttcattaca ccaccgccaa aaataattct atctggcgaa aacatcagtg
      601 ttgtattgta tgcacattgg gcaaggtaaa aggcttcaat ctgccaaaac gaatcgtcag
      661 ccaataaatt ctggcctttt acacccaacc gtttttcaat cgctggtcct gctgccatcc
      721 cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt ggatgtcgct
      781 tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc ccattgaaca
      841 aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct aaccctttgc
      901 cagaaccagc aacatattca ccatagcaag ccgcattgac atctgttgtc cattcaatcg
      961 gaatattgaa atattgcttt agcgttccta caaaattgaa attttgccaa gcaagttttg
     1021 gtgttgatgt gatatgaccg aaactcgttg atttgcattg aatatcaatt ggaccaaagc
     1081 agccgacccc gatgcctgcc aattgttcct taaaaggttt aaaaaaatca atgacctgct
     1141 tcattgtttc aactggagtt gttgtgggaa aactgactct ttccaaaatc gttaattcct
     1201 cagtaccgac tgcacagaca aatttagtcc ctccagcttc aattgcccca tatatcatca
     1261 taggttcctc ccttcgaata tgtgaaaaac acttcattaa cgcggcaagc gttctcgatc
     1321 agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccagtaag cgacactagc
     1381 aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac
     1441 cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg
     1501 tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc
     1561 tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt
     1621 cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc
     1681 ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa
     1741 ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta
     1801 atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg
     1861 ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc
     1921 gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat
     1981 tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccctg taggattaac
     2041 cactatcggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa agaaatcccc
     2101 taaaggactt tcaacagcag ggctatccga cccatcccaa taaattctta agattaaatc
     2161 acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca tatgacgaat
     2221 tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca gcggaattgc
     2281 gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt tacctttctc
     2341 gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt gggtaatatt
     2401 ttttagtata ctcatctttt tctcctttga atgtgaatcg tttttccctt ttctacgtga
     2461 agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga aatactcaac
     2521 tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt cttccaagaa
     2581 aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt caatccgaaa
     2641 tattgactga tcattaggct gacaaaaaga cccgatgccc agccgcattt gccgcagcca
     2701 aagtctcgca cgacttctcc ttgttgagcc tctaagcgat acggccacca ccaccacgaa
     2761 ccgtctaaat ctgccaaaca aattagttct tctaatcggg ttacgaaagc ttgagggttg
     2821 tgcagctcgc tcattaaaac agtgataaag ccaggaaaag tggcgccgga ttctaatccc
     2881 caagtgatgc ctttgatttc ctcgctatag gttggattga aagaactagc agaaaaggcc
     2941 atggtgttct gatataaatg atctttatta tctaaaaatt gataaaaagg catcaacgta
     3001 gtgtctgatt cttctccatc atgcatcaat aaattaattt ttccatccac aatcacatca
     3061 cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agtgcttcac tgaataggtt
     3121 gttttctcct catcaccgat tccctccaaa aattgctcac caaaaggacc atcgatagtc
     3181 atttgtttac aaattgattc ctttagttta caggcaatgt ttttgtatcg cttgctaaga
     3241 ctcagttgtt ctagggcttc aaaaatttct gcaagtccaa tacaggcctt ccaaagacaa
     3301 agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca aacagatgga
     3361 aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat tcttgaagca
     3421 ttcagtagaa cctgcggaca ccgctcaaag aattttgtat ccccagtatg ttcaaaataa
     3481 agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat acctcctgta
     3541 aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt tttttgacat
     3601 aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt attccaaatt
     3661 cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg tcgattcata
     3721 ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac tctatcgttt
     3781 ggcattgaca gctgcccaaa tagctgagca aaatagttca atgtttcctt cagccatttt
     3841 tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc cacaaaagca
     3901 atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt ttgagaaatc
     3961 aacacatttt gctgatccga atacttctct tgatacgaag gcacatcttt cactgaaact
     4021 tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat acttaaacgc
     4081 ttattagcca gtatgggagc aaaggatact acaatgacct taaacgtcga ataatgatga
     4141 atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc cgattctgac
     4201 aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt ttgagagagc
     4261 tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg ataaaaggtc
     4321 aacgtttgaa tcgtcccgca aacatcaagg tttgctaatg tcttttgatt acttacatcg
     4381 aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt tgaagtagtc
     4441 acatttccgt cgtttaactc agcattaaaa aatgggtgtt tgggtgaata aattacttca
     4501 ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct gcagcttcct
     4561 actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca aaaataattt
     4621 tgatgacgcc gactttttta tctagtaatt tgaaacagct caatgttacc aataacggta
     4681 caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct tcgcccttca
     4741 ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgca cctaccatga
     4801 tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc gcatacagct
     4861 tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat cgcatcaagt
     4921 aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt ccggcattgg
     4981 ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac aatgaatcgc
     5041 caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt tccacgtcaa
     5101 aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg acaaaggacg
     5161 cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca gctcgatctt
     5221 cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa ccaccgccgc
     5281 ccatcgtcac cggattcacc gaagcataca tagtcatcga gcgccaaaac atcttccgaa
     5341 ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga aatcctcctc
     5401 ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa cagatatatt
     5461 ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa ctgcggcaat
     5521 tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa accacacagc
     5581 aatattttta ctccaaagca tccgtaacag catcgccatc ccaactgccg gcagcagatt
     5641 tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga cgcttttgac
     5701 aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg catagtggac
     5761 aaaccacata aagaagtgca gattgatgat tcctttttga tcaccagatt ttgcaaattt
     5821 gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca ttaactgtaa
     5881 tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc ctcccatttt
     5941 gattgcgaac gctgtagcca gcccagcagc caaagaaggc tccgccgtac tagagattcc
     6001 gatattgacg acacccataa agatggcttc tagtgacgcc ccgacaatca accccgtctg
     6061 caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac tcagcatgga
     6121 catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga ctacaaatgc
     6181 atcatacata atttttctcc ttcctttttc taaaaatcac tcaaaataga gatagctgaa
     6241 cgtttttcca tcgccggtgt aggctgaaaa acggtatctg gataaagttc aactaattct
     6301 tttaagtctt gaatttctga cgtcgttaac atcacttcct ttgataaggt tttcttatct
     6361 tccgatggcg gtgtcatttt cccagcattc cccacgttca ttaattcgat ttccgttggg
     6421 aaagctttcg caacttttac cgcatctttg acattaggga ctaaaaccat caagcgcatg
     6481 tccttcgccc gcggatcttt taacaaatga atcgcgtcat caactgtttt aataatgact
     6541 ttgacattcg atggtgcagc catcttcaat gtcatctttt gaatatcgtt ggtagccgcc
     6601 tcatcgcttg ctactactaa cccggttaaa tttagactct ttgtccaagt catcgctact
     6661 tgtccatgaa ttaatcgttc atcaattcgc actgctttaa tcatttttct tcctcctaaa
     6721 aaaaatcttc ctcttcattt actacatgta cctcatcaag aatggtcatc tttaattcct
     6781 gttggcattt atttaccagt tcctgaatat cctcctttgt taattccttg gttgccaata
     6841 ctatttcgag cagaattggc agattaaagc cagacaaaat agttacagca atctctttct
     6901 ccaaaaaggt tttggtaatc ttctgattga cacttccgcc aaataaatcg gtaaaaacaa
     6961 aaatctgatc ctgctcgttc acctggttta caaatacatt caactcctgt tcaaaatcac
     7021 actcatccac ataagcatta atcaccgtaa tctcttgaac cttccccgtc agtaattgaa
     7081 tactgctaag aaagcctgaa gctaagtgac cgtgagtcgc tattagaatt ttcttcatat
     7141 ggttaattcc tttctttctg ttcactttta tataagcaat tcctgtgcca acttttaaaa
     7201 acattggttt aatcacgtta attcacatta aaaaaagtgt ctaacctaaa aaaaacagat
     7261 tagacacttt tttagacact ttatttttga atatacggtc ctaataattc atagagcagc
     7321 gaaagctcat aagcattgac tttaaaatta tatttcatct ccattgattg aaaaatactt
     7381 ctagaaacat aataaaattc atcttcatct ggtttcaaat cataattcgg ttcttcagat
     7441 ttttctgtcc gagccaagaa taaacgttcc atcatcaatg ccgtatgcat atagagattt
     7501 agttttacac ggccatttaa agtgaaatga tagtaatttt catatttaga aatgactgtt
     7561 tccacttcac tcatcacgat cgttgggttc agaaagttca aacggtccgc aattccttcc
     7621 aacgaaaaga attttactag ctcctgaatc aagcggttaa attctgccgg cttcatttca
     7681 ggactcaaaa tatgctccaa attggctgct ccatcaccgt ctaagatgtc ataaacatta
     7741 atattaggaa ttgaaaagga acgtggaaga tccgaagtcg tgatgaccaa ttttgtcttg
     7801 tcaaaataag tttcatcatt ttggtcgatt aattcacgta gttctctgta gtccttcgtg
     7861 aaaacttcga tggattcatc cgataaatga tgacgaaaaa catctttcaa tttgtccgaa
     7921 atccccaaac ccgacataca ggaaatgata atgttgctac tttgagaaaa gccttcaaag
     7981 tattgaacat tgattgaata cttgtctgct gcatcctccg caatctgttt aaatggcgca
     8041 ttcatcgtca tcttcaagcc aatatcaagt gcaatggaag tcgtcaaatt attcagaaga
     8101 agtaactccc cttttaaatc gttcttgatc tgtgtgtaaa tttgatttaa agagcccata
     8161 tctacgatca gcactagact atctgtattc acttgccgtt tcacgaaatt ccttgtcgat
     8221 tcgacaatct ctgacacatt actctctact ggcatatcca acgcttcaaa aacataattg
     8281 ccgcataatt gattcactac tgcttgaatg ctgctagccg tactatctcc gtgagcaagc
     8341 aacaggccct tcagctcgat ggattcatct acataatcag ataaagtcac gacaataagc
     8401 agtcttagaa agttctcgtc cttttctgag agcggtggta aatgctccaa gaactgatta
     8461 cataagtaag tcgtccgagg tagatttgca gctaatgtac gatttatccc ggttaagtct
     8521 gtcttatagc ttggggaaat gacataggat ctgctaataa tttctgcaac aggtccactc
     8581 gtcgtcaatc cgtacgtttt tcctaaaacg gatacccaga cctttctaaa catctgaatc
     8641 atcaattttc tactcattga taaaaaagaa tccttatcaa tgtgatgcac caaatgctgt
     8701 acttcattct ttatcgaaga aaaattttca ttgaaattac cttcatttat cagctgagta
     8761 attttttcca aaatcgaatc ataatcaaac ggtttgattc gatccgtttt tatcttttga
     8821 tttttcttta tcaataaact agcaatcggc aagctttcta cttcggattc cagcatattt
     8881 aactgaatca agatctcatt tttcccaatc gttaatggtt cttcttgtgt tcgactatac
     8941 acatccgcac aactgatttt aatgatattc ttcagcttac caatattccc actaaaggga
     9001 tgagataaca ttaaagacaa ggcttctctt gaaacaataa tatctttttg taaaacatcc
     9061 gcttcatttt gaaaaaataa ttgaatcagc tctaatcgtt ctcgaatggg gcgatcttca
     9121 aaagttggca acataacact aatttgaata cgccgatcaa aggtgtctaa taaataattt
     9181 tcaccttttt ccgtcgtggc gaaaataaaa cgaaccttag cagaatgcca atttacattt
     9241 tcaccgactg gacgatagtt tccagtatca ataaatagaa agagcttttc ttgattttct
     9301 ttcgataggc gatggacctc atcgagaaaa agatagccgt tgttggccgt tgccaataaa
     9361 ccatcggtat ccttttcagc ccccgtaaag gcccccttct tgtatccaaa cagtgttgcc
     9421 gaaagcagtt cgggattgtc cgcataatcg gcacagttta agatagcaaa tggtgcatct
     9481 ttttcaataa tttgctcatg gatagcgtat tcaaaaattt ttgtggctaa aaaacttttt
     9541 ccaacacccg tggctccatt tattaataca ctgagtccgt taggcggata tttgacagcg
     9601 gctatacatt tttgaatgac ctctctcaga cttccgtcat gaccaactac agaatgaaag
     9661 gcttcgttta tttgctccgt attatctaca accgcatatt catctaaagg cttccacaaa
     9721 acaggctttc ccggtaattt ctcgaccttt ttatcttcca ctaatctcgt caaataatgg
     9781 ctggcattct gacgagagat acctagattc ttcgaaattt tctcggcagt gacagaagac
     9841 atattccctg ttagtcgtat cagctcatca aaaactcgat ctttagcgct ctttttcatt
     9901 ttttcctcca gtttaatgtg tctaaaagca taataaacta tcggctgcaa tatgtctatt
     9961 ctatatttgt attccaattt ttactttgaa acacaaatag tcgaatattt gcctcagata
    10021 tctgacaaaa taatttatct accaatttag tttttatcta atatgatttt tatgtttttt
    10081 catag
//
LOCUS       DAYYNG010000102        10034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000102 DAYYNG010000000
VERSION     DAYYNG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.102"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..1043)
                     /locus_tag="ACHBFA_002018"
     CDS             complement(24..1043)
                     /locus_tag="ACHBFA_002018"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9395462.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="ACHBFA_002019"
     CDS             complement(1676..2680)
                     /locus_tag="ACHBFA_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9395463.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="ACHBFA_002020"
     CDS             complement(2693..3742)
                     /locus_tag="ACHBFA_002020"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9395464.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFFFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="ACHBFA_002021"
     CDS             complement(3759..4580)
                     /locus_tag="ACHBFA_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9395465.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="ACHBFA_002022"
     CDS             complement(4567..5346)
                     /locus_tag="ACHBFA_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9395466.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="ACHBFA_002023"
     CDS             complement(5363..5833)
                     /locus_tag="ACHBFA_002023"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395467.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="ACHBFA_002024"
     CDS             complement(5845..6264)
                     /locus_tag="ACHBFA_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395468.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9122)
                     /locus_tag="ACHBFA_002025"
     CDS             complement(6345..9122)
                     /locus_tag="ACHBFA_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9395469.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            9313..9537
                     /locus_tag="ACHBFA_002026"
     CDS             9313..9537
                     /locus_tag="ACHBFA_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395470.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         2925 a         2094 c         1653 g         3362 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaaaaaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaa
//
LOCUS       DAYYNG010000103         9927 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000103 DAYYNG010000000
VERSION     DAYYNG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.103"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..591)
                     /locus_tag="ACHBFA_002027"
     CDS             complement(79..591)
                     /locus_tag="ACHBFA_002027"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9395471.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(605..2902)
                     /gene="recJ"
                     /locus_tag="ACHBFA_002028"
     CDS             complement(605..2902)
                     /gene="recJ"
                     /locus_tag="ACHBFA_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9395472.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3044..3487)
                     /locus_tag="ACHBFA_002029"
     CDS             complement(3044..3487)
                     /locus_tag="ACHBFA_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM9395473.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3488..4276)
                     /locus_tag="ACHBFA_002030"
     CDS             complement(3488..4276)
                     /locus_tag="ACHBFA_002030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9395474.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4273..5214)
                     /gene="rnz"
                     /locus_tag="ACHBFA_002031"
     CDS             complement(4273..5214)
                     /gene="rnz"
                     /locus_tag="ACHBFA_002031"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9395475.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5391..5978)
                     /locus_tag="ACHBFA_002032"
     CDS             complement(5391..5978)
                     /locus_tag="ACHBFA_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM9395476.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6199..7512)
                     /gene="obgE"
                     /locus_tag="ACHBFA_002033"
     CDS             complement(6199..7512)
                     /gene="obgE"
                     /locus_tag="ACHBFA_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9395477.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7809..8819
                     /gene="gap"
                     /locus_tag="ACHBFA_002034"
     CDS             7809..8819
                     /gene="gap"
                     /locus_tag="ACHBFA_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9395478.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8904..9839)
                     /locus_tag="ACHBFA_002035"
     CDS             complement(8904..9839)
                     /locus_tag="ACHBFA_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9395479.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2730 a         2270 c         1716 g         3211 t
ORIGIN      
        1 atagagaatc tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga
       61 atagcttctt ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt
      121 gtcgcgtcca tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc
      181 taattgttcg atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc
      241 acaaatcagc acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt
      301 tccatattct aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg
      361 gactggggca aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg
      421 ggcttctggc ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt
      481 agtagcttcc cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat
      541 tccttttgac ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt
      601 cctcctaggt tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct
      661 caactttgat tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt
      721 ctgggctagc gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa
      781 acacctgtat cataaaaatt aataattttt cagggatacg caaatattga gctattgctt
      841 ttattttatg acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt
      901 actgttctct tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat
      961 aaacacgatt gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac
     1021 aatccataac aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta
     1081 gtccttcgaa aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag
     1141 aatcaaatgc taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg
     1201 cccgccaatc aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt
     1261 tcttacgccc gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca
     1321 gcagttcttc ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat
     1381 cggaaaggac aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt
     1441 ggacttgtcg aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt
     1501 tcagctcatc aattcgctct actgttactt catttggcag caatacttca tcaattctca
     1561 actcaggccc tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct
     1621 gtagtatagt gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga
     1681 agtaagtaaa taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac
     1741 tacgtccaga tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac
     1801 ccgtttcatt cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt
     1861 ttaccaatag atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct
     1921 gctctacgat tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg
     1981 cttcctcttc atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac
     2041 cgattgcatt tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca
     2101 tttttacgcc gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg
     2161 catcgattcc taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga
     2221 tcgttcctat cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag
     2281 ctactttgaa tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat
     2341 gacgagggtg aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg
     2401 taatgatcac gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc
     2461 cgttatcaac agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt
     2521 ggtttggtcc atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa
     2581 gtaattccag tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac
     2641 cgtaaacgag gattttttct ccactgatta cagcttcttg gattcggctg attgctttat
     2701 ccatatcgtg tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt
     2761 tgatatcctt ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata
     2821 atttcagttc acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc
     2881 aatcaaaatt tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat
     2941 cagcaaaaaa aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca
     3001 gcaggacctg atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga
     3061 agctttgttt gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg
     3121 cggttttcgc ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa
     3181 actcgcgttg ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt
     3241 ctgacatttc ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag
     3301 cagtcaaaaa tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg
     3361 gcgcagaaaa ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa
     3421 aaagaacaat aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt
     3481 ccttcaccta ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca
     3541 gcgtataaaa ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat
     3601 gtctcatatt gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga
     3661 ctttttccaa atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac
     3721 ctggattcac tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg
     3781 agaagccaag gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt
     3841 ttccagccat agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg
     3901 caaatttttg tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt
     3961 ttccaaggtc gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa
     4021 tatcgacgcg cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat
     4081 tgctgacgtc taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc
     4141 tgacaccttc tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg
     4201 ctgcttcata acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt
     4261 tatcttctaa attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc
     4321 tttcataatc gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc
     4381 ttttcctaaa tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt
     4441 tgcaacttca gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc
     4501 atctttatta aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga
     4561 attatgtgtt tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc
     4621 tacgaaatct tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt
     4681 cagtttgccg tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa
     4741 ttctccttta tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag
     4801 tttttttgta taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc
     4861 aatgaatttc agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta
     4921 ttcctctatc ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg
     4981 gctgctgatg agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat
     5041 tttaccgatt tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc
     5101 acagtcgaac aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac
     5161 atttctatgt tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta
     5221 cctgactttc tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta
     5281 agtcgagaat gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa
     5341 ttactataaa aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa
     5401 gatccagaac tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt
     5461 gcagcttctt tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct
     5521 tcataatcca agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg
     5581 cgattgtcct cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt
     5641 cctacaaagt cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg
     5701 atcattcctc cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc
     5761 aacattccga ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct
     5821 tctattttga attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct
     5881 ttaatcgaac gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt
     5941 tctacttgaa aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct
     6001 tcttgttatt tattttacct ttaattgtaa aataagaaaa gaaaagcact ctttttttcc
     6061 aataaaatag ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt
     6121 cttgaagctg atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat
     6181 ttgtttttat ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag
     6241 gtcgccatct ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg
     6301 acgagcaaaa cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc
     6361 gatcttctca ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg
     6421 accttctgga ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg
     6481 gaattctggc gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg
     6541 tcgagtcact ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc
     6601 cttgttcagc tgttctttga atttcactag attttccggt gcatccggca tatccatttt
     6661 gtttgccacg atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt
     6721 gttgatggca agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac
     6781 atgtaaaatt actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc
     6841 ttgcgaagct ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact
     6901 tgtagtaacc attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg
     6961 acgagcagat gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag
     7021 tccaacgtct gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc
     7081 gttttcagca atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc
     7141 accgcgtcca ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag
     7201 agcaccagtc tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc
     7261 tgaaccacga ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg
     7321 gcgattgaac cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc
     7381 gccgccacga ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg
     7441 aaatgcaacc attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa
     7501 aaacatggac ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc
     7561 cacaataaac aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca
     7621 gcaaaaagat tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt
     7681 ctgctaccaa gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt
     7741 aattgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa
     7801 cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt
     7861 tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa
     7921 gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca
     7981 cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag
     8041 aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat
     8101 gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac
     8161 gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg
     8221 atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag
     8281 cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag
     8341 ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc
     8401 gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa
     8461 tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag
     8521 tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc
     8581 aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc
     8641 gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc
     8701 aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca
     8761 atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat
     8821 ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt
     8881 tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact
     8941 tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca
     9001 tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca
     9061 aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc
     9121 actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact
     9181 tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca
     9241 tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct
     9301 ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc
     9361 gattttccat tgttttctag cactttgttt atccactgta catagtcttt acgctctggc
     9421 catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg
     9481 acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc
     9541 atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc
     9601 gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa
     9661 ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg
     9721 tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac
     9781 actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata
     9841 aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct
     9901 ctactatgca atgttcattt ttataac
//
LOCUS       DAYYNG010000104         9916 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000104 DAYYNG010000000
VERSION     DAYYNG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.104"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..543)
                     /locus_tag="ACHBFA_002036"
     CDS             complement(<1..543)
                     /locus_tag="ACHBFA_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9395480.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(555..1247)
                     /locus_tag="ACHBFA_002037"
     CDS             complement(555..1247)
                     /locus_tag="ACHBFA_002037"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9395481.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1266..1550)
                     /gene="macP"
                     /locus_tag="ACHBFA_002038"
     CDS             complement(1266..1550)
                     /gene="macP"
                     /locus_tag="ACHBFA_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9395482.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1563..2126)
                     /locus_tag="ACHBFA_002039"
     CDS             complement(1563..2126)
                     /locus_tag="ACHBFA_002039"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9395483.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2354..3016
                     /locus_tag="ACHBFA_002040"
     CDS             2354..3016
                     /locus_tag="ACHBFA_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM9395484.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3140..4336
                     /locus_tag="ACHBFA_002041"
     CDS             3140..4336
                     /locus_tag="ACHBFA_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM9395485.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4437..5813)
                     /locus_tag="ACHBFA_002042"
     CDS             complement(4437..5813)
                     /locus_tag="ACHBFA_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9395486.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5850..6716)
                     /locus_tag="ACHBFA_002043"
     CDS             complement(5850..6716)
                     /locus_tag="ACHBFA_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9395487.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6805..7830)
                     /locus_tag="ACHBFA_002044"
     CDS             complement(6805..7830)
                     /locus_tag="ACHBFA_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9395488.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7848..8522)
                     /locus_tag="ACHBFA_002045"
     CDS             complement(7848..8522)
                     /locus_tag="ACHBFA_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9395489.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8703..9599)
                     /locus_tag="ACHBFA_002046"
     CDS             complement(8703..9599)
                     /locus_tag="ACHBFA_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9395490.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(9673..>9916)
                     /locus_tag="ACHBFA_002047"
     CDS             complement(9673..>9916)
                     /locus_tag="ACHBFA_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9395491.1"
                     /translation="YVLPTGEAAFTEFIISYGSYNEEIQIVGVENPGPERVPNMKRGS
                     LPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         3012 a         2153 c         1736 g         3015 t
ORIGIN      
        1 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
       61 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      121 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      181 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      241 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      301 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      361 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      421 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      481 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      541 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      601 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      661 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      721 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      781 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      841 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
      901 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
      961 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1021 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1081 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1141 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1201 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1261 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1321 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1381 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1441 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1501 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1561 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1621 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1681 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1741 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1801 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1861 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     1921 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     1981 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2041 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2101 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2161 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2221 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2281 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2341 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2401 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2461 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2521 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2581 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2641 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2701 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2761 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2821 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     2881 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     2941 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3001 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3061 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3121 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3181 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3241 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3301 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3361 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3421 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3481 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3541 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3601 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3661 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3721 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3781 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3841 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     3901 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     3961 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4021 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4081 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4141 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4201 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4261 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4321 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4381 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4441 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4501 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4561 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4621 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4681 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4741 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4801 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4861 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     4921 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     4981 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5041 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5101 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5161 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5221 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5281 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5341 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5401 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5461 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5521 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5581 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5641 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5701 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5761 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5821 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     5881 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     5941 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6001 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6061 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6121 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6181 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6241 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6301 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6361 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6421 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6481 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6541 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6601 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6661 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6721 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6781 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6841 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     6901 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     6961 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7021 accgatccct gaatctttaa ctgaaaggct gactccttgt gcttcctttt ctatgctcac
     7081 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7141 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacaa tctcgtagcg
     7201 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7261 tagcctatat tcttggatga ggtagtcccg cgaaaaactg tctagtcttg cataatacaa
     7321 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7381 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7441 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7501 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7561 ttcttctgtt cgtccaccgc tcaaatagtt ttgcaaaggg gaagtttttt gtatgtcggc
     7621 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7681 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7741 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7801 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7861 tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg
     7921 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     7981 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8041 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8101 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8161 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaaggt
     8221 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8281 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8341 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8401 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8461 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8521 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8581 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8641 atttaatcgc ttttgtcttt tcaatacagc ttaaatttat tatgttattc ttaacaattg
     8701 gtctatgatc tgttattttt ccttagttgt ttttgtcttt taaatttcca ataacaccaa
     8761 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8821 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgcctt
     8881 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     8941 agatcctgct tttctctgat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9001 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9061 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9121 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9181 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9241 atatagattg ctccgatcat atgttgttcg tagtattctt ttcctggatt tcggacattt
     9301 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9361 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9421 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9481 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9541 aataattgta ataaaagact gatttgggtt tgaggtctat tacctgttct ctttttcatt
     9601 ttcttcacct tcttatttaa aaaaactgca gcaatcggtt ttataaaggt tgctgcagct
     9661 tatttttgtt gcttatactt cagacttcat tttaaattta cggtaccagc tgtacgctcc
     9721 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9781 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9841 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
     9901 tgttggaagt acataa
//
LOCUS       DAYYNG010000105         9829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000105 DAYYNG010000000
VERSION     DAYYNG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.105"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..723
                     /locus_tag="ACHBFA_002048"
     CDS             244..723
                     /locus_tag="ACHBFA_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM9395492.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            756..2984
                     /locus_tag="ACHBFA_002049"
     CDS             756..2984
                     /locus_tag="ACHBFA_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9395493.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3113..4756
                     /locus_tag="ACHBFA_002050"
     CDS             3113..4756
                     /locus_tag="ACHBFA_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9395494.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4829..5374)
                     /locus_tag="ACHBFA_002051"
     CDS             complement(4829..5374)
                     /locus_tag="ACHBFA_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9395495.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5397..5882)
                     /locus_tag="ACHBFA_002052"
     CDS             complement(5397..5882)
                     /locus_tag="ACHBFA_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9395496.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5872..6684)
                     /locus_tag="ACHBFA_002053"
     CDS             complement(5872..6684)
                     /locus_tag="ACHBFA_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM9395497.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6823..8355)
                     /locus_tag="ACHBFA_002054"
     CDS             complement(6823..8355)
                     /locus_tag="ACHBFA_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9395498.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8464..9495)
                     /locus_tag="ACHBFA_002055"
     CDS             complement(8464..9495)
                     /locus_tag="ACHBFA_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9395499.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3271 a         1832 c         1893 g         2833 t
ORIGIN      
        1 tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa catcatcttg
       61 gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc
      121 aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt
      181 agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa
      241 ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat
      301 aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact gggccttgct
      361 cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa
      421 aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta
      481 gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca
      541 gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat
      601 accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac
      661 ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat
      721 taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa
      781 accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga
      841 caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa
      901 tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca
      961 aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg
     1021 acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc
     1081 aagaattact agaagctatt taccaaacag atacacttga agctgtactg acactgatcc
     1141 agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa
     1201 ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc
     1261 gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc cgagatgcct
     1321 ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag
     1381 gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag
     1441 gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc
     1501 ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag
     1561 aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg
     1621 ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag
     1681 gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc actgattcag
     1741 atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg
     1801 acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa gaaagaacca
     1861 ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct
     1921 tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag
     1981 aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg
     2041 aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg
     2101 acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca
     2161 tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa
     2221 aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc
     2281 ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg
     2341 aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa
     2401 agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt
     2461 caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat
     2521 tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa
     2581 gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg
     2641 aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag
     2701 cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg
     2761 gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg
     2821 ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca
     2881 aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg
     2941 aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac
     3001 gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt
     3061 ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca
     3121 agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga
     3181 tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa
     3241 agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa
     3301 tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt
     3361 tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat
     3421 caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc
     3481 gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac
     3541 atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca
     3601 tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact
     3661 attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat
     3721 ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt
     3781 tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta
     3841 cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt
     3901 agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca
     3961 agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg cttggcaaac
     4021 ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc
     4081 cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc
     4141 tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat
     4201 gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta
     4261 taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac
     4321 aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc
     4381 tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca
     4441 agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac
     4501 caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta
     4561 tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc
     4621 acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa
     4681 ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata
     4741 taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt
     4801 tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt
     4861 taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga
     4921 caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata tcgataccgg
     4981 cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag
     5041 aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt
     5101 ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta
     5161 gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt
     5221 ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa
     5281 caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca
     5341 attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa
     5401 tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt
     5461 ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat
     5521 aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct
     5581 aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg
     5641 tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga
     5701 ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag
     5761 atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt
     5821 aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc
     5881 atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat
     5941 cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt
     6001 aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc
     6061 gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata
     6121 gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt
     6181 tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag
     6241 agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg
     6301 aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt
     6361 gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg
     6421 aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt
     6481 cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat
     6541 aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc
     6601 aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc
     6661 gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt
     6721 ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa
     6781 aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt
     6841 taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc
     6901 aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg
     6961 attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt
     7021 atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat
     7081 agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg
     7141 gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc
     7201 ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat
     7261 atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt
     7321 catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt
     7381 atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc
     7441 atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa
     7501 agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg
     7561 ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat
     7621 atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat
     7681 caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa
     7741 tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga
     7801 atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata
     7861 agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa
     7921 ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt
     7981 tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt
     8041 tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac
     8101 agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt
     8161 cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta
     8221 agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt
     8281 ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt
     8341 tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata
     8401 atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt
     8461 gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat
     8521 cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt
     8581 taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag
     8641 ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg
     8701 agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc
     8761 tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt
     8821 cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg
     8881 tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc
     8941 aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc
     9001 aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc
     9061 tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc
     9121 tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta
     9181 atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt
     9241 atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag
     9301 ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc
     9361 ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg
     9421 ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct
     9481 agttttgctg tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat
     9541 ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta
     9601 ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt
     9661 cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg
     9721 aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga
     9781 aaacattttt tcagaatcga ttggctctcc atttattcaa taatggttt
//
LOCUS       DAYYNG010000106         9822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000106 DAYYNG010000000
VERSION     DAYYNG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.106"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHBFA_002056"
     CDS             complement(105..629)
                     /locus_tag="ACHBFA_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9395500.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBFA_002057"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBFA_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9395501.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBFA_002058"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBFA_002058"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9395502.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHBFA_002059"
     CDS             complement(2351..3031)
                     /locus_tag="ACHBFA_002059"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9395503.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHBFA_002060"
     CDS             3157..4026
                     /locus_tag="ACHBFA_002060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9395504.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHBFA_002061"
     CDS             4052..4567
                     /locus_tag="ACHBFA_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9395505.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHBFA_002062"
     CDS             complement(4614..5177)
                     /locus_tag="ACHBFA_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9395506.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHBFA_002063"
     CDS             complement(5232..5411)
                     /locus_tag="ACHBFA_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395507.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHBFA_002064"
     CDS             complement(5488..5997)
                     /locus_tag="ACHBFA_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395508.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHBFA_002065"
     CDS             complement(6093..6473)
                     /locus_tag="ACHBFA_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9395509.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBFA_002066"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBFA_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9395510.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHBFA_002067"
     CDS             complement(7736..9013)
                     /locus_tag="ACHBFA_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395511.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHBFA_002068"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHBFA_002068"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9395512.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
BASE COUNT         2731 a         2032 c         1759 g         3300 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca at
//
LOCUS       DAYYNG010000107         9810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000107 DAYYNG010000000
VERSION     DAYYNG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.107"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..2153)
                     /locus_tag="ACHBFA_002069"
     CDS             complement(33..2153)
                     /locus_tag="ACHBFA_002069"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9395513.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="ACHBFA_002070"
     CDS             2383..3561
                     /locus_tag="ACHBFA_002070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9395514.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="ACHBFA_002071"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="ACHBFA_002071"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9395515.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="ACHBFA_002072"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="ACHBFA_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM9395516.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="ACHBFA_002073"
     CDS             complement(4914..6167)
                     /locus_tag="ACHBFA_002073"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9395517.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="ACHBFA_002074"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="ACHBFA_002074"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9395518.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSRGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="ACHBFA_002075"
     CDS             7745..9091
                     /locus_tag="ACHBFA_002075"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9395519.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="ACHBFA_002076"
     CDS             complement(9399..9785)
                     /locus_tag="ACHBFA_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9395520.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1781 c         2009 g         2892 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttttctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 agggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat
//
LOCUS       DAYYNG010000108         9773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000108 DAYYNG010000000
VERSION     DAYYNG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.108"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..357)
                     /locus_tag="ACHBFA_002077"
     CDS             complement(<1..357)
                     /locus_tag="ACHBFA_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9395521.1"
                     /translation="MLTKKELIDHIDETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIIDILDLKVDPELGGILRGKEGYFPAYHMNKEHWISVDLRKI
                     EKLDEPDCRQKLSVRTA"
     gene            501..1940
                     /locus_tag="ACHBFA_002078"
                     /pseudo
     CDS             501..1940
                     /locus_tag="ACHBFA_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1947..4499)
                     /locus_tag="ACHBFA_002079"
     CDS             complement(1947..4499)
                     /locus_tag="ACHBFA_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM9395522.1"
                     /translation="MNWYKLTVEDTLKNYQTSTEGLSQEERQKRLTDQGYNEIEARQQ
                     TKRWKKIVKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADVRLIEVHDLLVE
                     ESILTGESEASEKNHTVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFSYGMDWPILLSAMIAL
                     VVSMVPEGLPAVLTMILSVGVNEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEETRVLSNMKNCQELKTKEEQKTENLSGNPTELALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHTEGKGAVIYVKGAPEVLLAKSTLSSSEQEAWGQTATAFAKK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSEEELIRQVPKVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILILALILNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVALLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKSLFLSLGILALLQGMVIFLPS
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREFSR"
     gene            complement(4612..5943)
                     /locus_tag="ACHBFA_002080"
     CDS             complement(4612..5943)
                     /locus_tag="ACHBFA_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9395523.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIALAGPVVI
                     LVYLICGVAIYGLMRAMGDLFLANSKFNSISDFIDYYLGEKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIV
                     FFILLGIGLSVSYFHTQSIGIKANFGQLNELLPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYVGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQSGAAIVNLMILVSSLSSANSAVYSTSRILYQTAKEKSLPPWFCALSKKGVPLHGIL
                     ITGTLFLAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHIQFVKEKKRNTIP
                     KYKDLALLVFFVAIAFSLLFERMTRFIPFLLVIWFVLLSFVYKRISDRNIN"
     gene            complement(5909..7387)
                     /locus_tag="ACHBFA_002081"
     CDS             complement(5909..7387)
                     /locus_tag="ACHBFA_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9395524.1"
                     /translation="MKDAGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKNWGFIVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLTFDMAKIGGKIGVWLGLY
                     VPLTMMLLLGLAAWIKVGIVPTGTLGNFSWDKVIPDSTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGISVLRLRY
                     THAKLNRPFRIGKKENKSNLGVWAIVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7694..9529)
                     /gene="tdc"
                     /locus_tag="ACHBFA_002082"
     CDS             complement(7694..9529)
                     /gene="tdc"
                     /locus_tag="ACHBFA_002082"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9395525.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMADGWGHITADGSIANLEGLW
                     YARCIKSIPLAVKEILPEKVKNMSEWALLNLSVEEILEMTESFTDEEMDKVKAASSRR
                     GKNIQKLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     DQKIPILGVVAVVGTTEEGQVDSVDKIIQLREKLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHYVTIDKEVYEGFRAISEADSITIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVEAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLEQLHFTVKGKTIEVYPLNHPDFNMVNWVFKVQDC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFIERMGLSKEE
                     WQKEQQITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIK"
BASE COUNT         2963 a         2088 c         1657 g         3065 t
ORIGIN      
        1 agctgtcctc acagacagct tttgtctaca gtctggttca tctagttttt ctattttacg
       61 tagatcaaca gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt
      121 tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa tcaaggatgt cgatgatcct
      181 atggtcactt gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca
      241 ttttttgctt ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc
      301 tggagacaaa ttgtatgttt catcgatgtg atcgattagt tcttttttcg ttagcatctg
      361 cttccatctc tttcttattg attcacttta ttcaaagata ccacgtaaga atagaaaaat
      421 gaatactggt tgaaagatca gttgaaactt gaccaccgtt tattttctcc gtattcttag
      481 aatagaaggg agtctgattt atgtattcac taatgaaaaa aatcattacc gaaaaagata
      541 ttcgacgtca tgtttcttta gtcgaacaat tgctgaatca tacaaagata acggtaaatg
      601 aattagctga aatcataggg acaaccgaac gaacgatttt ttctgacctt caatctattc
      661 gctctcagct gcccgaaggc tgggacatcc tcagcgatca agcaggaata agtttgcaaa
      721 accatcaaaa tttattaaca aatgaccttt gggagatttt tttcaaacaa tcagtcagtg
      781 ttgagctgct gaaaaatctg ctttttacaa aaaaagttgc tgtccctgat tttctagcgg
      841 atcacgggct ttcttatggg acattaaaaa gacatgtgac taaaatcaat cagcgtttag
      901 caagttacga cttgcagatc gatttgacga aatatacagc ttgtattctt ggaaaagagc
      961 gtgcgattcg tactttctat catcgattgc tgattccttt cactcataac aactattttt
     1021 tcgaggatta ttcgatccat gaatcccatt attttcaatt tttaagaaac ctcagtcaaa
     1081 ctgaactagc tgttgaaaca gaagagattt tcggaacatg ttggttcttt atcaatacga
     1141 tccgtataaa agcgaattgc cgattagatt cttctatcca tatcaacagc acactctcct
     1201 ccctgtatga ctctgcattg aaaaaattgt atttaacgga aggtatttat cttaaagaca
     1261 ctgagctttc ttttgcttct ttctgctttc tggaaagttg gaattacaac aataactatg
     1321 gacaagaaat cgctcgctgc ctgcatcatt ctccattttt agaggtcctc gaaacgtttg
     1381 tagaggaact agcttcagag ctgtctcttg atcaactaaa aaaaacatct ctgactgata
     1441 acttggctct cctcgtatta aaatatcatg aatcgccgat actgatttaa cctttggaac
     1501 gccaatacca tcgtttttta gagacgtatg atcagcaata tgcacacttg tatcagcaga
     1561 aagagaattt actgaacgat ctaaaaaaag aaatggaaat caatgaacca aattactttc
     1621 ttcaactctt gtctttactg atccaacaaa cgattttttc tatcaaaccg aatttgttca
     1681 atgtctattt tattttccaa ggagaacctt catggaaggc gttcttgcag caggaattaa
     1741 aagattactt gggaaaacgt gtctgcttct tacctatcga actaaccgat ctttccacta
     1801 tttcttttga aaaaacagat atcttgattt ccaatttccc tctggattct ctagatatcc
     1861 ctattgtata catttcttct attccaacga aaaatgagct caatcgtttg actgaactca
     1921 ctttcaaaac ttttttatag taacggctag cgactgaatt ctctagctgt tttttctttt
     1981 ttgcgaaaca gtttattcat gatccctttt tctatttcta caactaagaa tagaagaatc
     2041 acatttgccc caatcaataa ctgttgtgtc atcgttaaac ttactgtacc gatcagctgc
     2101 tggccgcttg gcagaaaaat aaccatcccc tgcaataaag caaggatacc caatgaaaga
     2161 aaaagcgatt tgttttgcaa aagaccttgg ttcaacgacg gcttggacaa ctctctgcaa
     2221 ttgatcatgt aaacagcttg agctaagacg atattttgca gaagaatggt ttgctgtaag
     2281 ctttgccctt caaattgcat agccaataaa taggcaggaa ccatgatcag tagtgcgaca
     2341 tagatgatcc gaaacaagtg ataacctgat aggattcctt gctttggatc tcttggtgga
     2401 cgcttcatga tttctggatc tggcgactcg aaccctaatg cataagataa agtgattgtc
     2461 gttaccatat tgacccataa aatctgtact ggggacaaag gtagcggccg attcagtatc
     2521 agcgccaaaa tcaagatcaa tccttgagct aacgcagttg gtaagaagaa attgatggtc
     2581 ttttgcaagt tatccaaaat gcgccgacct tcttttacgg cttttgcgat cgtatggaaa
     2641 ttatcatctc ctaaaaccat atcagctgcc tgtttgctta cttcactacc tttgattccc
     2701 atagcgattc ctatatcggc tcttttcaaa gcaggtgcat catttacgcc gtctccggtc
     2761 attccgacga tttctccatt tttctgcaaa gctttcacga ttcgcagttt gtgttctggt
     2821 gtcgttcgag caaagacatc gacttttggt acttgtcgaa tcaactcttc ttcagacata
     2881 agatccaaat cgatcccttc taaaacttta gcagtatgtt ttaagccaat ttgttccgca
     2941 atcgcttttg ctgtgtcttt atgatccccc gtaatcattt tcactgagat ccctgcttgt
     3001 tggcattcct ttacagcctt gactgcactt tcttttggcg gatcgatcaa cccggcaatc
     3061 cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt cctgtttcga atctactttt
     3121 ttgtaagcaa atcctaaaac acgctgacct tttttcgcaa acgctgtggc tgtttgaccc
     3181 catgcttctt gttctgaaga agaaagagtt gatttagcca ataatacttc tggcgcacct
     3241 ttgacgtaaa tcaccgctcc ttttccctcc gtatgcatcg ttgccatata tttgtattca
     3301 gagctgaacg gaatctttgc ttctattttt ttcaaaggca gattggctgt ttcagtgtgt
     3361 cgaatcagtg caagttctgt cggattgcca cttaaatttt ctgttttctg ttcttctttc
     3421 gtctttaact cctgacaatt tttcatatta gacagtacgc gagtttcttc ctttgctgct
     3481 acatccacaa ctgtcatttc attttttgtc aatgtccctg tcttatctga acagatcact
     3541 gtcatagaac ctaatgtttc tacagaaggc agtcctttga tgattgcctt ttcttttgcc
     3601 atttcattta ctccgacaga taggatcatg gtcaaaacag ctggcaatcc ttcgggtacc
     3661 attgacacca ccaaagcgat catagctgaa agaaggatag gccaatccat gccatagctg
     3721 aatgttgtaa aaacaactaa gaacaagatc aaacaaagaa tgccttgaaa aatttgtttg
     3781 ttcaatcggt tgatcttctt gatcagaggc gtcgtttgtt gttcgaccga ttgcagtgct
     3841 tgattgattt taccgatttc tgtcgtgttt cctgtttcta caacgacacc gactgcagaa
     3901 ccagattgta caagtgttcc tgaaaaagcc atatttttct ggtcaccgat tgattcttct
     3961 gctgccaaaa cagtatgatt tttctcagat gcttctgatt ctcccgtcaa aatcgattct
     4021 tccactagta gatcatgtac ttcgatcaac cgaacatctg ctggaacaac gtctccagca
     4081 cgtaagacaa tgacatctcc ttttactagt tctttcgcag gaattttttc ttgcatcccg
     4141 ttcgatagta ctatcgcttc ttgtcccatc agctgcttca aaccattcaa cgattcttct
     4201 gcttttcttt cttgccaata actaacgagt ccgttgacga tcactacgaa taaaataata
     4261 cttccttcga tgtattcgct cgaagcaaat ttcaaaaaag aggcaatgat caacacgatc
     4321 atcagcagat cagtaaagtg ctttactatt tttttccatc gttttgtctg ctgtcttgct
     4381 tctatctcat tatatccttg atcagtcagg cgtttctgcc gttcttcttg cgacaagcct
     4441 tctgttgatg tttgatagtt tttcaatgta tcttctaccg tcaatttata ccagttcatc
     4501 ctatcccctc ctacttaatt gatactttta ttatacgtag ggagatcttt caggcgtatc
     4561 gattatttca gagaagtctg aaataattcg ttttgccgga tatatttagt cttaattgat
     4621 atttctatcg cttattcttt tatatacaaa acttaagaga acaaaccaga taactaataa
     4681 aaaaggaata aaacgtgtca ttctttcgaa taacaagctg aaagcaatgg ccacaaaaaa
     4741 taccaacaaa gcaaggtctt tatattttgg tatagtgttc cgtttctttt ctttaacaaa
     4801 ttgaatatgt gcacataaga tcgaagacca cacaaataga aaacaactcg ttgtcatacc
     4861 tgccaaaagc tgaaaaaaca atcgactatc agcgacaaaa aaatgtagac aaagacctgc
     4921 aagaaataac gttcctgtaa tcaaaatacc gtgtaaaggt acgcctttct tagaaagcgc
     4981 acaaaaccaa gggggcagac ttttttcttt tgctgtttga tataagatcc tgctagtact
     5041 gtaaaccgca ctatttgccg aagacaaaga agaaaccaga atcatcagat tgacgattgc
     5101 tgccccgctt tggcaatgga tcatttgcaa catagtgact aaaggacttt cttttaaatc
     5161 taaacaatgc catggaacaa cgaataaaat cgacagaatc gttcctacat agaaaaagag
     5221 gatacgccaa ggcagacagt tgattgcatt tttcaaattc ttttgtgggt cactcgtctc
     5281 accaactgtt aatccgatca tttcaattcc agcaaaactg aataaaacca taggaaatga
     5341 agcaatcatt ccagcaaagc caaacgggag taattcattg agctgtccaa aatttgcttt
     5401 gattccgata gactgggtat gaaaatatga gacgctcaaa cctataccca ataaaataaa
     5461 aaaaacaatc cccaaaatct tgatgattga gaaaaaagat tccaattcgc caaaccattt
     5521 gacggcaaca agattgactg ccattaagat cattaatgca ctacctccgc ttatcaaagc
     5581 tggtacatgt acaaaccaga aatggatata caatcctatt gctgtcaatt ccgcaagtcc
     5641 agctccaagc cagcaaagcc aataagtcca attgacaaaa aaggcccact tttcacccaa
     5701 atagtagtca atgaaatcac tgatagagtt aaatttacta tttgctaaaa acaaatcacc
     5761 catagcccgc atcaagccat aaatagccac accgcatatc aaatagacga gaataacaac
     5821 tggtcctgcc agtgcaattg cctttgctga acctaagaat aatcctgttc caattgcgcc
     5881 acctagtgcg attaactgga tatttcgatt agttagagtc cgtttcattg ccggtttttc
     5941 catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc caagaaggtt
     6001 tctttatttt ttcaatacat agtggtacta caaacaaaat gacactgatg gctgtgactg
     6061 caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaag ccgattgccg
     6121 caaataaaac aataacgatc gcccagacgc caagattgct tttgttctct ttttttccaa
     6181 ttctgaatgg acggttcaac tttgcatgtg tataacgtaa tctcaaaacc gagatcccca
     6241 taatgacata gacgatacaa tagattacag tagttgaatt aactagcatc aaaaatgctt
     6301 gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg acagattgag
     6361 tcaaaataac ggatttggat acgtcaaatt gatttgtctt ccaaaaattg aattttggtg
     6421 gatacgcacc ttttctagca ctttctgtca ctgtctttgc aggtcctgat gcccaagcag
     6481 ataattggac ggccacaccg atgaagacaa gcaaactaaa cagattcaca ataagatgcg
     6541 gcaaaccgag aatttggcaa taaatcgaaa tagattgtgc gatattattc aattccattt
     6601 gaccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg aaaaatataa
     6661 aaatcagagc agcaaatact ccgcgaatat acgttttcac tgggttcttc aatcgagtga
     6721 tatacacgga ggacatttcg atgccagtaa aaataaacat gatcggcgca aaataaacaa
     6781 atgaggatgc agtcgtacta tctggtatta ctttgtccca tgaaaaattg cctaatgttc
     6841 cggttggtac gattccaact ttgatccacg cagcaagccc taataagagc atcatcgtca
     6901 aaggcacata tagacctaac caaacaccga tttttcctcc gatttttgcc atgtcgaatg
     6961 ttaaattcaa aaaagtgatg atccaataaa caactaaaat aacaactaat gtaaatttgc
     7021 tgttcagccc aagcggcaca ttatcgataa tattaccaaa caacggagcg agtgctgaag
     7081 caaccatcac catccctgga aacatttgga cccataacag ccaggagaca atgaatcccc
     7141 aattttttcc tagagcattc gatacccaaa gttctggacc acctttttga ggaaacattc
     7201 cggcaaattc accagagatc aatgagatcg gaaaggcata taacaatacg gcaaccagca
     7261 tgtaaaaaat catcgtccag ctcgttgctg ccacatctgg aatatttcgt acacttgcta
     7321 caagggcaca ggtcatcccg ataaaggtca tggtcgtgat ttgttttgat gaaccagcat
     7381 ctttcatctt tattccttct ttctttcaat agcgacatgt ttttcgtttt cttttatttt
     7441 tgttcaataa caaaagtgac aaggcttcat cgattcagta caaaacccag tatcaccgaa
     7501 acatccattg aacgaactta gtgaactggg tttgatacgt gaaacgaacg aaccaactat
     7561 agaaagattt ataaaaaaga atgtcttgct gaggaatata tgaaaaacag ggggaaatac
     7621 aactgttttg atgaggcaaa taattgtctg ttctccatat gtctcttgct attctttcac
     7681 acaaaaatca gttctatttg atgatttcgt ttagtttctt ttccattgct ttggcgattt
     7741 cttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg atagccgcac
     7801 gtagcagagt gatttgctgt tctttttgcc attcttcttt cgatagaccc attctttcaa
     7861 taaaacggat cggagaatca ccgtaatctt cttgtgtaaa tgtcgtatga gaagtaataa
     7921 accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt ttttcattta
     7981 attcattgat agcgttcaaa tctgtacaat cttgtacttt aaatacccaa ttgaccatat
     8041 taaaatcagg atgattcagt ggatagactt cgatcgtttt tccttttaca gtaaagtgta
     8101 attgttctaa aaattctctg aatctttgag ctgcttcgat tgatgcacca atcaattgac
     8161 catatccggt cacattcaat ggcaatacac gatgagctgt ccagactgca gcagccgtcg
     8221 ctcctgcttt tgagccttct aaaatataag cacctagcat atctggtgct tcgactgatt
     8281 tttcaaacac atatggtgca aaataagaaa tgatatttct catattttta tgtttgatta
     8341 cgattcctcc agcagcataa gggacatagc ccatcttatg cggatcaatc gtgatagaat
     8401 ccgcttctga aatagctctg aatccttcat acacctcttt gtcgatcgtt acgtaatggt
     8461 ggaatacatg atgctcttca aagaattcag ctaaggaagc atatggcaca aattctcctg
     8521 cttcgtttaa aaacagtgaa cgcgcgtacc cgccatatgc agcgtcaaca tgtaaataga
     8581 aatagatacc ttcatctttt aacttttctc ttaactggat gattttatct acactgtcta
     8641 cttgtccttc ttctgttgtt cctacgacag caaccacacc tagaatcggg attttttgat
     8701 cagctaattc acgaattgtt ttttccaaag cgttgatatc cattcgataa tcctcttgaa
     8761 ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc ttcatccaag
     8821 aatagtgttt tgtttgagga accagccatt tccctaattt ttggatattc tttcctctac
     8881 gagaagaagc ggctttgacc ttatccatct cttcatccgt aaagctttcg gtcatttcca
     8941 aaatttcttc aacagacaga ttcaaaagag cccactcaga catgtttttc actttttcag
     9001 gcagaatttc tttgacagct aaaggaatcg atttgatgca acgggcgtac cataatcctt
     9061 caaggttagc aatagaacca tctgctgtaa tatgtcccca tccgtctgcc atgttgaata
     9121 gtgaggcaaa atcctgtcct acttctgctt ccatctgcga agttgccata gaagattcta
     9181 gtgcaacgtt attgggattc catagcatcg cataattgta agctaataaa gcaggcatca
     9241 gtgtttcagc gttcatttgt ccccagtaac ggcctgctga atgccagggg atcgatcctg
     9301 cgcgtaatcg ctgactgact tcatccaaga cttctatcat atggtcgcgg gtggctagat
     9361 aatctggagt tagcttatct ccttcgctaa ttgcaggcat atctgaagga atataatttt
     9421 ctctccagcc aagatgttca tctaccatct tgttcaacaa cattttgtaa actggtccat
     9481 tttcagcttt gtcgcctata aagactgcct tgatatccat atctttcatg tttcttccct
     9541 ccatatttcg ttggtcaatg tctcgcagat acatgtgctt atctgtaaca cctcttcact
     9601 atagcgccaa aaatgagaaa atttttttta taacatcaga ttcacgtaga atatttcaaa
     9661 caaaaataaa taaagtcgga tttttctgat aattgaacgt ggaaaaacga acaatttttt
     9721 aataaatcag acttctaaaa tggatagtct aaaaaaaacg aacaaataac tta
//
LOCUS       DAYYNG010000109         9684 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000109 DAYYNG010000000
VERSION     DAYYNG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.109"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            749..1552
                     /locus_tag="ACHBFA_002083"
     CDS             749..1552
                     /locus_tag="ACHBFA_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9395526.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2137..2592
                     /locus_tag="ACHBFA_002084"
     CDS             2137..2592
                     /locus_tag="ACHBFA_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395527.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2711..3769
                     /locus_tag="ACHBFA_002085"
     CDS             2711..3769
                     /locus_tag="ACHBFA_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM9395528.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3811..5760
                     /locus_tag="ACHBFA_002086"
     CDS             3811..5760
                     /locus_tag="ACHBFA_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9395529.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5763..9404
                     /locus_tag="ACHBFA_002087"
     CDS             5763..9404
                     /locus_tag="ACHBFA_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFM9395530.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3476 a         1391 c         1970 g         2847 t
ORIGIN      
        1 taaattcaca tactttttct ttttttggat atattcggaa taccacatcg ctttttgtcc
       61 acgtttgcat cgctttttgt ccacattcac atcgcttttt gtccatgttc gcatcgcttt
      121 atgtccaccc taaaatctat ttaagattga tataaagcct ttttggaggt tgctaaagtt
      181 ttaaagatta aaatatatat tataaatata aataaaaggg gaagctgaag cttcctcctt
      241 ttcctttttt tcaattattc aaagttacac aagcaaaata aactcctaga tagtgttagt
      301 tttttttaag attttttatt tttaagattt tgaaataaga gaggagtaat cctcctttat
      361 taaatatctg ttaatatatc tgttaaatat ctgttaatat atctgttagg ggaggcttaa
      421 tctcttggga gagtaagtat taaaggggta taaaggtaac acttttggac agtaaaggta
      481 acaaaagtgg acagtaaggg taacagtttt ggacagcaaa ggtaacaaaa gtggacattt
      541 gaggtatcac actaggataa gacgttacct tcggttttct tgatttggaa ggcgaaaaaa
      601 tagaaaatta gattagtaac aaatctttgt tgactgttac ctttagttgt gatttaattt
      661 aggtaacaag cagaaaaaat tgtttccttt aattagtacc agattaacat gattaaagga
      721 gcgcaaaaag aagattggag agtaatcaat gtctcaagaa gttaccgaaa agattattga
      781 tagtgatgta gatttagtat cagaacaaac aaatgcaatt gtgcgttata acaatggtct
      841 gaatacaaca gctttgcgag gatttaagcc tgttgaaatg gatgtgtttt gggctatttt
      901 atcaaaaatg aaacgtcaag gaacagcaga agtaactttt gatttcaacc tctttcgtta
      961 tttggtcaaa tacgacagaa gagaaaagga cagtttttat tctgtgttaa aagatttgag
     1021 tgacaaacta ggtaatttaa cctaccgttt tgaagattca gacgtctatg aacagctgtg
     1081 gctttttcaa aaattccgga tagataaaca gaaggaaaca gttacaatta aggcaaatga
     1141 ggaatttgaa tttatcttaa actcaattgg caaaaacttt actcgttttg aacttgataa
     1201 ttttacaaaa ctaagaagta gctacacaaa agaattgtat cgtcatctca tgcaatacag
     1261 aaataaagag tggcgaaatg gttattgggt agtcaaagtt gaagatttta gaagagcatt
     1321 aagtatacct tcaagttata gaatgacaaa tattgataaa agaatactag agcaggcgaa
     1381 agaagaattt ttagctcctg atgaaaacgg aatacaggta tttgaaactt ttgattatga
     1441 aaaaatctat gcaaagaaag gaagaagagt tgatcggctc agattcactt ttagtgagcc
     1501 agaatcaaac ttgccaacca tttcaatgca taattggtta gaagaagatt gagaagaagc
     1561 aggttgtaat tttaatacaa cctgcttctt taaaaaagta tgggtaacac ttttggacaa
     1621 taaatgcaag aagttttttt gtaagcaagt ttcagtctaa ataatatttg aaagaatggc
     1681 taatctatac aaattttaac aactatgggt aacacttttg gacaagtcaa gaaaaaaaac
     1741 aatttttctt aatttgttga tcgactaaag gtaacacttt tggacaacac agaataaaaa
     1801 gtccggtgat atcagttttg gtaggaaaca tcttaatatc tttgtgagat tgaaggtaac
     1861 atttttggac aagtcaacta tttgttaaaa gttaatatag agtaattaga taagtttttt
     1921 cttttcgtct gataaatgga taatattagt tattttattg ctaattagtc tttcatgatg
     1981 actgaaatag ctaaaataaa tatctcgttg gataattgct gatactttaa gggatatcta
     2041 aatttgctga ttgatttatt tatgttatta tgatattata ataacataaa tgtgttacgc
     2101 ccgaacctca ttttaaaaaa atgaaggggt gagacaatga atgctattaa ttggcatgaa
     2161 tttgttaact atatggagag cttactagat gatctaaaaa gttttagtat tgctgatgca
     2221 gtaaagcatg ggttaccagt tagtacaacg atgaaattac gaggattgaa aattccagat
     2281 gaaattatta agctgagaac taaaacaatt attgaaaccg caaaagtcgt tgacaatatg
     2341 aaccatgaac taaaaggcaa gaaaacaact tacgagaaag attttgaacg ttttttaaaa
     2401 tctgttattg gaaaatatct aggtaaaaaa tctttcagtg aattgagaga tgaagagatt
     2461 gtacctactc caatggttag caaaatccga aaaaacaaag ccgaggggaa ttactatctt
     2521 ggtacaatag caaaaatcat tgattatttg gaaaataaag agcaaaagaa aggaacgaag
     2581 aaaggaagat aatttttgtg ttaaatatta atggggaaga atataccgaa atgattcaaa
     2641 atcccgaacc tagaattacc gaaaaaacgt ctgattgttt gctcttttat gaaagggggc
     2701 agacaaacta atggaaaaaa acgtcaattc aacatctacc aaagatcaat acagtattat
     2761 tggagggttg cttggtgata aggataagag aacacgtcac ggattacgca ttatgcttgc
     2821 aacttttcta ggagtgcttt ttttattaac aggcttgggt agctacattt atcaattagg
     2881 aaactataat tattatctcg tgaacaatac aacacctata ggctcttcat tggagtttgc
     2941 tcgatcagga gcagagatca ctatttcaga tgtgtggaca gatcaaaaca gagatgtaac
     3001 agtagtaaaa ctcggttact cgcaaaacgc tagaaagttg ttatctacgc aaggaaaaaa
     3061 ctacaactta tatatggcaa cgaaagaaaa agatcgtcct aaaaagcttg aaatgagcta
     3121 tgggatgtta agtactgatg gggatgcttt cttattccta aaaggtaaat tagatgaaag
     3181 agcttatcaa gtttttattg caaaccaagt atcactaatt tctggtgatg atacagctga
     3241 cgatgatacc aaagtcaatg tgaatgaagc agattcagtt acaaaagcac tttctcaata
     3301 ttctatgaat gatgttaata atgaaggagt aatttctttt gggaaaaaag caaaacctgt
     3361 taatactcct gataatatca atttcagaat aaatccatat tcagattcaa ctaatgtcta
     3421 tcaaggaagc tttcttacca aagatggcga tattgattat tcaaaagttg tagcagttac
     3481 aagtaagaat aatgttgtaa gtaagttaga agatcagtta aatgcttcaa atactaacat
     3541 caagaaaatt gaagctacaa aagctgagta tctggaacgt ttacaacaaa atcctgacga
     3601 taaagattcg caagaaagta ttcgtcaact agacagtcga atggagaccg aaattaaagc
     3661 aatcaatgat acacaaaaat tgattgatca atacaaaaat gctgacttta acaaagattc
     3721 ttttggagac atgaagacga catttaagta cctaactctg actgactaga agtaaattat
     3781 tcttatttta gaggaagtga acacaaacag atggaaaata gtaccgagtt ttttaagaat
     3841 attcaaactg aatttaatca tgctctcaca ccaatgaaac caccgaaaca taagttagtg
     3901 ggacgtgatg acgaactcca tgagttgtca ctgatcttga atagaccgga aacaccgcaa
     3961 gctatcttgt taggtgaagc cggaacaggt aaaacagcac ttgcaagtga atgggctgaa
     4021 agacagcgac aagatggaaa gaaagtacaa gtatacaaat tatcaatcgg acaattgcgt
     4081 gttgggggag atgatgtcct ccaaacacgt ttagaaacat taattccaag attgaaagaa
     4141 tatgagcgaa ttttaaaatc tgaggacaaa gaaaatgaat ttgtcctctt cattgacgag
     4201 tttcataaag ttgttagctc ttttggtagt ggcaataaaa ttgggggtga ttcgctaaaa
     4261 gaaccgttag caaatgaatc acttcaattg attaccgcta ctacaagaaa agaatatgaa
     4321 aactatatca aaagagatga tgccttaggg agacggttac aaccgttaga attgaatgaa
     4381 ctaaaaagcg atgttgtcgt cgatattttg agacaatggc taaatacatc ctctgttaaa
     4441 gatgatcctc aggcttcttt taaattatcg caaaggattc ctgatgaatt acttcaagaa
     4501 atcgtacaag caaacaagct ttatcgtgag gatttgtccg aaccggctaa atcaattgcc
     4561 gtaatggagg atttgattgc aaaagatcga gtagacggaa atgcttataa tcgagaaagt
     4621 ctattgcaaa tatttggtaa gtataatatt cagctagatt ctgattttga tatggaaaaa
     4681 cttcaagagg aattaaagtc cataaaaggg caaccgttag cagtttatac tataacaatg
     4741 ttgtttaaac gtatgaagct tggatttgat cgttttaaag gtcgaaagca accaaaagca
     4801 actgctctgt ttacaggatc aacaggggtc ggtaaaactg caatggctaa agccgtggca
     4861 agaggacttt atggcgatga aaataaagtt ctcttagtta atatgccgga ctacaaaaca
     4921 aaagatgatg caatgcgttt tagacgactc ataggtactc atgtaaggca tgatccttct
     4981 gcgattattg tgtgtgatga atatgagaaa gctggtgtag aaacactcga tgccttttta
     5041 tcaattttag atgaaggtag gataagtttt actgaaagag gatgggatga tcgagaagtt
     5101 cacgacactg tttcattgtc aaatacaatt ttatttgcga caacaaatgc aggtgcggag
     5161 atgttcgatt cgttaaacag atatagctca ctatctcgtg ttacaaacaa ggaaaattct
     5221 aatgaatata gcactgaact aaaaagagaa taccgaaaag tcgcatcagg gatcaaacaa
     5281 gccttgattg gtgaaggtct aaagcctgaa gttattaacc gatttggacg aatagttcca
     5341 ttttgggctt tatccgaaca aactcttctg gaaattgctt ataatgaaat gttgaagagt
     5401 gctgaattag tacgaaaaca tttaaatata gatttacgtc ttccacctag gaaagaaaga
     5461 gtatatggtc aaaattttta tgcagacgat ctctccatgc acttagtttt tgacatggct
     5521 tcacaagagg attctaaatc agggggtgca aggaatatct tgcatttagt tgaatctgag
     5581 tttgaagaag cgattactga agcaataatt acttatccaa acgcaaaaat attcgatgtt
     5641 aagttgagtg aagccggagc gtttgaagga aaaggtgatt ctaatgctga aattattgca
     5701 aagccttccg ttgctgaaag taaagaaaac aagattgtat tttcgattgg aggggcgtag
     5761 ctatgaaatt caagaaatat ttactacaaa accttagaat aaaacgatgg gtcgtaagag
     5821 gtctagttat tggaggagta ttagcagtta ctttaggcgc tccttttgga atgaatgatc
     5881 caactcctga caatcaagat agcaaacaag aaacagttat aaataagaac gttgcatatg
     5941 cggaaagtaa accggcagaa caaggaatgt ggacactata tgctcgtatc ctaatgggag
     6001 ataaagaagt taatgatagt gccgctaaaa ctggacaaat gaatttgtta ggacaaggtg
     6061 gtatcagtgc cgatttccct tattctgaca tttctaaaca aggtaattcg attgcagggg
     6121 gtaatgcagg tactcaacta gcatctactt tagcaactta tagttactac ggatatattg
     6181 aaacagtctc aggaaatgct attgccagtc aagcatcatc cattctagga agtattggta
     6241 ggacgattgg tagttttatc ggatcaatta gtttaatcct ttctaatatt gctgaatcca
     6301 taaatgaact ccttggaaaa tttttcatga gtatgaatat attttctctt atggggtggg
     6361 gagaaggtgg atcggatgta tctaatccgg tatctaaagc attaactagc ttgcttaaca
     6421 atttgggatt aaataatgaa ttatttaaag cgatagcaag cttatctatg ctctttttca
     6481 cagcaatact aggttatcaa ggaatgaaat tattgtcgaa agatggtgtt aactcgaagg
     6541 gattatcacc agtacttgaa aaatggattg ttcgtatttt tgtattgttt atattcttac
     6601 cagtggcagg atcgctattg ggagtttata tcaacggcat ccagtcagca aaaaaagatg
     6661 gagatatacc aacaaatgtt gtttctgact acatcattaa cacaagactt tgggcttcta
     6721 ctcaaaatct tgctccatca tctgatactg gtggtgatgt acctgacgga aagcctacat
     6781 ccgaaatcaa gttcgttgat acagattatg aaccatctaa aaaatctgca atgataagag
     6841 cgatcaatac tagcagttat gccagacaaa ataatgatct agattctaat tcagtcggta
     6901 tggctctatt aaaagaatgg tcatcgaatg atactttcaa tattaatact tatgccggtg
     6961 atgttaatgt cgccgaaaat gatgcttata tgggaagagc gcctgctgtt tggacaggct
     7021 ataatttagg ggatggaaaa gaaggaagtg caattaagaa accaaccaat ttaaatggat
     7081 atatttggtc agcaaatcaa aatgtgacag acgacaatcg caagcctgat ggaaaaggaa
     7141 cagcgcataa ctttgatgct actcaacctt ggggcgttgc taacaatgca acttttagca
     7201 cacagtcggt agctttattg ctccaatcgg cacttgatac atcgggagct aagttttatg
     7261 cgtataatat cgctccttca ggaattcaag gcagtgctaa gaatatgtca acagtaaaaa
     7321 cagaatggcg tacagcatcc ttagttggtc atggagctat gggtaagttt ggctcgtggc
     7381 tagcgatgaa cgctgaatca atcgttttag cagttcttta cttagcatgt gcttgggcga
     7441 tgttcaatgt gaatatcgtg aaagcattta aacgtgaatt tattcggatt tgggaagtag
     7501 ttctaacagg atcacttgca tcaggagcag gtgtgatggc aatttcatta ggtgttgtta
     7561 caacatctct gatcggacta caattaccta tctttctgat tacttttatt cgtgcatttg
     7621 gtgatactgc tgtgggattc attcctaata cattagttgg cgatggcgta gcaaatattg
     7681 ttcgatctgc tatgatttta ggtgtaggat atcttttagt ctttaaaaaa ggcgaaaaag
     7741 taaataattt gattaatatg cttattggct taccacttga agtagcgttt gggtttgatg
     7801 aacgtgctaa gttattattt ggtaatagaa atgatgctgt gcgagttgtg gacggcgttt
     7861 ctaaagcaat caagaaaagc caaggagcta aaaatcccta ttatgatggt ggcaacagct
     7921 cagtaaaaga tatggttgca ggaggtcttg gagcagttgg agcgcaaaaa atggctgaac
     7981 gtttgaacaa tatgggagat agcaatgctg atgctaagtc cattgctgat aaagtaattc
     8041 ctgaaacacc agaaggaatg tcattcaacg gtggcggagt tggttccaca atcaataata
     8101 cctctttcaa agatttagtt cgtcaagatc aactacgtaa taactcccaa caatatattg
     8161 atcatttagg acgtatggtt gatgaaaacg gaaactttgt cgatgaaaat ggtgaaatat
     8221 taaaagatag taaatacggt aaagatgcta aaggcaactt tattgacgaa aaagggcgtt
     8281 tggtcgatga agatggaaac cttatagata ctaatggtaa accattggaa gatagtgaag
     8341 atggtaatat tggtatggat agcgaaggac gtttaattga tgaaaaaggt cgcttagtcg
     8401 atgaagatgg aaatcttgtt gacgaaaatg gacgcaggtt gctaaatggt gattttggtc
     8461 atgatagtaa cggacgttta gttgacgaaa aaggtcgttt agttgatgaa gatggaaatc
     8521 ttgttgatga gtttggtaat aaacttggta aacacagcga tggatataat gtgaatggag
     8581 atttagtcga tgaggatggg aatatcattg agacgagaga tgcgcttgtg gcagatcgaa
     8641 atagagaact cgaacaacaa cggcatacgc caattgctgt tgcaattcct gagaatgaag
     8701 aagacgagat tactttagat caaactcatc aagaactgag gaatgaaatg agtggtgagg
     8761 acgtgtcaga cagagtgcat cgagaattag gagatggagc atctggtgaa agtcagtcaa
     8821 gagagcttaa cagcaacttt gataatagtg ctattcggaa tgacacagta cttaaagaca
     8881 gagaatcgct aacaaaagaa acggataaag aacaatcaga tgtgaagaaa aagttcattc
     8941 ctactcctaa tgttaatcaa acagataaat atgaaacaaa tattggatca ggaatgtcga
     9001 aagacaatca atcaattaga gaacctgaac tgacaaataa aaataataga acaaataatt
     9061 ctaaatctac ttcagataat tcagggaaat ctaatcaatc aaccaagaaa acatcaacag
     9121 ctaaatcagc cgttgaatat ctatcaaaaa ctggtaaaaa tgtttacacc tcagcagata
     9181 aagctttagc aaaagctcca aaaggtagtc tagcagggat aacctatgaa gctaaaaacg
     9241 aagctattaa aataggtaaa ggaacggcta aggttgccaa ggctactgct acaggagcat
     9301 ccaaaactgg taaagtagtg aagaaatcta ccatgtcagt caaggactat attgtaaaat
     9361 acggtaatca aaaatcaact gaaaataagt ctgaagacga gtaaaaagaa ggagcattga
     9421 tttgctcctt ctttttactt tgatttctaa ctgaagatgt caaggaaaat ttctatttat
     9481 gcaaaaaaat tattttaaat tgtctataaa tgaattaaat atttactgtt caaagttgta
     9541 taattaaaaa gggcttatgc tggtaaagaa cagaattatt atctaaacaa aaacttgata
     9601 aaccagcatt tttagccaat aaaaaaagtg aaataattta atggaaaatc tcttttaatc
     9661 atcgaaaatt tatgttatta tgat
//
LOCUS       DAYYNG010000110         9662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000110 DAYYNG010000000
VERSION     DAYYNG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.110"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..242)
                     /locus_tag="ACHBFA_002088"
     CDS             complement(<1..242)
                     /locus_tag="ACHBFA_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9395531.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNEL"
     gene            complement(235..1827)
                     /locus_tag="ACHBFA_002089"
     CDS             complement(235..1827)
                     /locus_tag="ACHBFA_002089"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9395532.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2337..3062)
                     /locus_tag="ACHBFA_002090"
     CDS             complement(2337..3062)
                     /locus_tag="ACHBFA_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395533.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3216..3542)
                     /locus_tag="ACHBFA_002091"
     CDS             complement(3216..3542)
                     /locus_tag="ACHBFA_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395534.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3579..3944)
                     /locus_tag="ACHBFA_002092"
     CDS             complement(3579..3944)
                     /locus_tag="ACHBFA_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM9395535.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3981..4196)
                     /locus_tag="ACHBFA_002093"
     CDS             complement(3981..4196)
                     /locus_tag="ACHBFA_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9395536.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4325..4660
                     /locus_tag="ACHBFA_002094"
     CDS             4325..4660
                     /locus_tag="ACHBFA_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9395537.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4736..6733)
                     /locus_tag="ACHBFA_002095"
     CDS             complement(4736..6733)
                     /locus_tag="ACHBFA_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395538.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6831..7106)
                     /locus_tag="ACHBFA_002096"
     CDS             complement(6831..7106)
                     /locus_tag="ACHBFA_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395539.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7338..8072
                     /locus_tag="ACHBFA_002097"
     CDS             7338..8072
                     /locus_tag="ACHBFA_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9395540.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8111..9253
                     /locus_tag="ACHBFA_002098"
     CDS             8111..9253
                     /locus_tag="ACHBFA_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9395541.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3166 a         1809 c         1411 g         3276 t
ORIGIN      
        1 attaattcat tatatttttt ttcagtaatt ttatatcttg tttcgtaatt atcataaata
       61 gcattttgtt gttcatagac aacaaaattg ctattagata caaagaattc tttctttaag
      121 gcactcccaa atggtcccct ccttactcca tcttttttat caagtaagca ttcaaactta
      181 cgctgttccc aagcttccgt aaatcctggg aaacgaattt ctggttgtat atcattactc
      241 atgtttgaac acctccagcg caccttgtaa ccacgcttca ttttcaggtg ttgtttgcag
      301 ggaagagata gcttctaaaa gttctttttc tagcacttgc tgttcttgtc gtatcttctt
      361 gatgtcattt ccgacatgaa ccatatcaat tggttcttct tcttcaaacg tatctacata
      421 gcgtggaata tttaagttat agtcattttc tttgatttca tcaaagtttg ccaaatgagc
      481 gtatttctcg acatcttttc tttcagcata ggtttcgagg attttttgaa tgttttcttc
      541 cgataattta ttctgatttt ttcctttgac aaattctcga ctagcgtcaa tgaataatac
      601 gtctcgagtt tggcggttct ttttcaatac aatcaccgtc gttggaatcg atgtcccgaa
      661 gaacaagttc gcaggcatac cgatcacagc ataaatactg ccgtcttcta gtaatttttg
      721 acgaatgacc ccttctgcag cgccacggaa taaaactccg tgtggcaaga cgatggccat
      781 agtccctgtt tccttcaaat gatagaaacc atgaagaaga aacgcaaaat ctgctttgga
      841 ttttggcgct aattttccat aacgattaaa ccgagaatcg tctaagaagg tcgtatctgc
      901 tgaccaatta gccgaataag gtgggttcat tactactgca tcaaatgtgt aaggttcatc
      961 tgttggccaa tccttattca acgtatcccc attgcggaga ttcatttctt cggcatctac
     1021 cccatgtaaa atcaagttca tttttgctag attgtatgtc gttgtattta attcttgccc
     1081 atgatattta acgttatctg ggtgagttaa gtagttacgc acatttaaca tcaatgaacc
     1141 tgaacccatg gttggatcaa aaacactaaa gaatcgtcgt tctttttgat ccaacgtcac
     1201 gatttgtgcc atcatatcag aaaccatatg aggcgtgtaa aattctccgg ctttcttccc
     1261 tgcttctgaa gcaaattgac tgatcagaaa ttcataagca tccccgatca cgtccccatc
     1321 gtgacctaaa acttccacgt cattcaattt cttaatgact tcggtaatcg tcacatttcg
     1381 ttgttgctca tctgttccta gtttttttga ttggagatcc acatcatcaa ataacccatt
     1441 aaattgattg taggtagatg ccaattgcac aaaggctttg tttagatcat tcaattggaa
     1501 cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggcg aaatcgcata
     1561 ccccaaaata tcaacgattg ttgcaatcag atcttctttt gattcttcat ctgaaagtaa
     1621 ttcgcgatac aacatcgttt gtttgcttac cgtatcatat tcctctaagg attcatctgc
     1681 taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa tcagtcccaa
     1741 taaataattt ttatattctg acgcatccat tttgctacgg agattatctg ccgcactaaa
     1801 taatttttga tttaattctg cacccaaaaa gatacactcc tatctcttat ttaaagttgc
     1861 tatataccct cttattttag catactccct tttagaaaaa gcaaaaaaaa aaagaaagaa
     1921 acttattctt gctaataaac tacgcgaaag gagtctaacg ttgtttgata taacttagac
     1981 tgatcgttaa cttatttttt atatttctac atagttatct ttagaaccat ttcagataca
     2041 tatgaaacaa ttacatatga ttttttcata aaaaaacagt actgtctagt agtataaaat
     2101 atacaaattt cttaaaaatt ttttcactaa aatcactacc tgaaacagtc taaaacactt
     2161 atatatcaac gtttaatgaa actatctccg tttcatttca gatacaaata aatatagtcg
     2221 ggctccaaat ccgaaaaaaa atacccccag tagcttatcc taagctactg gagatgtata
     2281 agtacggtca tataacaacg gggtagggta aataaatgaa aaagatggaa gaatcattat
     2341 tcaataactt ttttaaatac ttcgttgtaa tcagttacct gtccgttctt aaataccaca
     2401 gaataatctt ttttcccatt ttcaagtaac cgaagtggaa tatctttacc aactttctct
     2461 gtcactgcat tactgatacc tgttagatta gctaataaat ctgataaaga ttgagcatct
     2521 gtagaaatct ctgtatcagt atttatcgtg attcctttat cttcatttac aagtttccaa
     2581 gtaggtggta tagctgtgta tgtttctaca aatgaattaa gtttttcatt aagtgacttc
     2641 tcttttatat ttccatctaa aaactctgta tatatggctt gagctatact aaaacaaagc
     2701 tggccttgtt ctaataattt tacatcatta gttagtaaag ctgtgttcac gttatcaatc
     2761 gcttcaagaa gagtatcttt ggtgtcccta acagctttag gtgtgccttc tggaatttcc
     2821 ctacttgata cctcgtccat tttatcttcc ataaacccct taaaatcata tctttctccc
     2881 aaatccatag aagtttttga gaaagctttc atttctgagt ctatttcttc aataatattt
     2941 aacatgtctg atacatattg tttaggctga ttcgttttta atctatatgt ggtaaatatc
     3001 tcaattcctc caattatcaa taaaattgcc aaacaaatcc ataataattt tttctttttc
     3061 atttttcctc ctatacactg ctgttataac agtgactaat gtaaagattt tcccagcaat
     3121 catcagacta ttatttacag ggaatcaaaa aaactcctta gtaagcttac acctttagga
     3181 gttcctgttt gaacggtatg cataatacat ttaaatcaat ttaacattaa cacctcagta
     3241 tatggtatct ctacatatac acggtctttt tttggatagt actcgacatt tatattgata
     3301 gcacttccat tgtctaacgt agctttagcc cggtacgtgt tatcaccatt atcatgagtg
     3361 atcgtcacat catcacatgt ccgatttaag ttatactgtt cttgtaaaat ctgagttacc
     3421 aatggtacag atgacttctc tagtccagat gaacccgtca ttcttgaaaa aattatacct
     3481 ataatagcca atacaacgat acctataatg acaaacttct tattcatatt tactttattc
     3541 aactttttat ctcctatctc caaaatgatt ttctctcatc agtaactcat tgacatcccc
     3601 aatagtacac caaagatggc tccagctacc cctgctatca tcaaaatcgt acccatttgt
     3661 tcatctttct ctcgatataa gtaacccata ataacagcaa cagcaccaaa taccacagga
     3721 ataaatgcta atgatactac catagaaatc caaccaataa tcaaatacag cttccctgtc
     3781 tcttgtccat gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt
     3841 actgtgtctg ttccggttgt ctcatctgtt tcttgtaatg ctccgcaaaa ctggcaatac
     3901 ttactttcat ttgttatttc tttaccacat tttctacaat acatgtcttc tcctcctaaa
     3961 atcattcaat tgcttattag ctaactgttt actttattaa atacatcaga aaacagctca
     4021 tttattggta cacttaatat ctcagaaagc attttcattt gatagggttt aggaatcgtt
     4081 ctccctgttt gccacgaact tacacattgc tgtgtaacat tcattttttt ggctaactca
     4141 gtttgggaaa gtcctttttc ttttctgtat cggctgagat tacttacttt tttcatgatt
     4201 ctcccctcct gttttattat atacaacaat atgatgtatt tcaactcata aaatatataa
     4261 atacttcaaa tgtcattaca aatacaacaa cttgttgtac tctactgaga aggggggtgt
     4321 attaatgaaa cagaagcaat atgcaaaggt cattagagaa aagcgaaaag cactcgggtg
     4381 gacacaaaaa gaattagcta aaaaaatctt ctctacacaa caggcagtag caagatggga
     4441 aaattctgta acagaaccta acctcgatag cttaacagct ctttctagag ccctaggaac
     4501 ccctgtaagc cattttctag acaatgttga catagattat gaggaagagt ttttggctct
     4561 ctatcgctct cttagtactg aagatgcaat tcgaacaatt gactatatga agctattaaa
     4621 aaggcaagaa aatgaacgca atcagctatt gaaagattga aaaataaata acccaccgaa
     4681 ctttaacggt gggtgtattg agatgaaata agattaagag ctatggataa cctgtctagt
     4741 ttaatataat tgggtctctt ctattcctta ctctccgtcc atcatctctt gttacacttg
     4801 taaattgata aataggaact aattcaactc ctgataatat caattctttt attacttcat
     4861 ctatccctgc ccctttcgct agatatacta aaaaactact gcttgctttc acattgtata
     4921 gtttcttaat atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc
     4981 tgttttctat taaagcattt ttcacgctgt tatccctgac atttcgtagg cgttcacaaa
     5041 cagtgttaat aagttctttt tctgtcatta gatatgtccc ttgccacgat aagccctgta
     5101 aaacttcatg tgcttcatct aaaatcaatt gtttttcctt gctaagttct gatcgggaat
     5161 tttgaggaaa tactctttta gcctcttttt cgttaaaaca tatcttaacc cattcataag
     5221 aaaaatattg atatgtaaaa tgaaattttt ccagaagtgg aaggatatct tcattcaatt
     5281 tttgaatcac agaagttgca ttaaaaacgt ctaatcgata gacattgctt ctcttatgtt
     5341 taaagtttcc taacgctttc tcttccttta acctatttag gtattcgagc atcttcaaag
     5401 gaacatcttc atcatctaat ttttttatca atcctataaa agtcataagt gatagtaact
     5461 tattacattt atctatgtca taagttttac catagtaaga actcattttt ttagctaatt
     5521 gttcccccga caaaatacaa gtcacacttt ccttactatc atcaaaaaaa ttaacatcag
     5581 aaagcaagac atcatagaca cgtcgcattt cctctagata attccttatt gtcctgaaaa
     5641 gtttgtcttt cattgattca agagtgtcaa aaagtacttt tgattcctct ttcaattttc
     5701 gaaaatgtgt aggatacaag tctaggttct ctaatagcca attaaatgtt tctacccgac
     5761 tgtctttctt taaaattagc atcacaactc caataaaatt ttcactgata tcacaattac
     5821 tggaatgaca ataaaaaatg gaatgcccag ttttactggt aaaaatacta gcagagggat
     5881 tatgatcgtt gtgaaacaga catcttaagt ttttacttga cttaagtaat ttattcatat
     5941 caatggttaa caatctttca taaatctcat taaaagacat gtcggaggaa tcaaacaggc
     6001 agtctttaaa ccactgtttc atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat
     6061 cggtttcatt gactagttca tactctctcc ctgaatcact ttgacaattt ttagaacatg
     6121 tcatggtaaa atcaatcgga agctcagtaa tatctaacat gttgtcttca ttaattatta
     6181 tcactgcatc tgacttatat cctccaaaaa acattctatt cgaagcattt acattgtcat
     6241 cacacccggg gatgttcttt ttcaagtatt cgtaaacagc aaccatttta ccatagtcag
     6301 taattgcttc ttttaataga aagacaatct tgtatttttc tagattttca gagtaccgaa
     6361 tacttttttg tatcgcaaac gcagaattat gaactaaatt gatagataaa accttttcta
     6421 tgcttaagta tctactatca tctttactca aatctactac cttccccttc tcatctttaa
     6481 cagtgttatc gacatccaga ataattactc tggtttccac aaaactctcc ttgtcattgc
     6541 ctttattctt tgtcttgctt ggaataaatg attgtgatga atttagtatc tctacaaact
     6601 catctcttgt aacttctcta tagttatcta aacacctatc tcttacatca gttttttctt
     6661 tttttgtaac tggcttctct aataattttt ctttatctac ataaattgga atattaatca
     6721 ttgatgtttc catttgtgtt tcctccttat aaaattctgt taaagactaa aacatacaga
     6781 taataaaaac aaactcttta ttactatatg ttttagtctt atttgtttac tcaaccttta
     6841 taccgttcca tgaaactatc aatatcagat tttttaaatc taatgaccgt atcaattttt
     6901 ataacaggta acccgtattg agaaacatat tttctaatcg ttcctactga tacacctaga
     6961 tattcacttg cctgctgata gtccatatac tctagagtga ctccagaaat aatgtcttct
     7021 agcataggct ttagcactgt aattatctct ttcacttgtt gttcaacttg tatagaaaca
     7081 atgctatcaa tgatttgtag agacatattt ccccctcctt tcgtgattgc ttgaacaaat
     7141 tttctaagaa ttatcttcgt attttttgct caaaataaaa gtattttgtt attaaccgat
     7201 taatcaaaca aaaaatgatt accagttcat cgacatacac ataatacctc gataaaaaag
     7261 gggtgtcaat agttttgtaa aatttattta caacacccct tttttgaggt atactaattt
     7321 taggaggtgt ggtcaatatg ataaaacgtc caccaataaa ttatttagaa cgaaaaaaga
     7381 tactcggaac aaaaataaaa gctataagaa agagtaaaaa attaacacaa ccagcatttg
     7441 gtcttatgat taacaacggt caattaattg acaaaaagac tatctacgaa tgggagaagg
     7501 gaacctattt accaatacca gagcgattat cccgaattgc tgatttggga aatatgagta
     7561 tagaggagtt agtttgtggc aatgtagaag aatatatttt gggaattatt ctttatagag
     7621 atagcattgt actagatggt ataacatttc cagataaaaa cctgttccag catcttcgtc
     7681 aacagttccc accagtacac tctaatttag atacatggct ggatagatat tccaaattag
     7741 aaccagagat gcaagaattt attgctaaca agacttgtaa taaagttaaa aatgaaaaaa
     7801 ttagtctttt caatatctta aaaatagaag aattattcat caatgctatt gtagaagaat
     7861 ttgacaataa cattcttttc ctcactagca gtatagaaga actactagaa agaatggtag
     7921 atgaatggct acctattcaa ttgaaagaca tgtcttatcc cgaagaagct gtgagagaaa
     7981 ttactgataa tataaacaag ctagaacaaa cgatttcatc aattgggaaa aaatacacta
     8041 agaaaaaaat gaagggaggt gataccatct agtggtaagt actccttgta aactaagagg
     8101 agatgatttc atggcaaaaa tatcaaagta caaaaagaaa aacggtcaag tggcgtggat
     8161 gtataaagga cacattgcca ccgatccacg aactggtgaa aaaataaata ctacgagaag
     8221 aggttttagt agcaaacaag aagctcaaaa agattatgac gagtacagac atcaaatatt
     8281 atacggtgtt aaaaagaaag cacctgatat gacatttgaa gacctgtata atgaatggat
     8341 aactcatcag cgtaccagtg ttaaagcttc aacaatcgca atctcagtaa gatacgcaaa
     8401 taatcaaata cttccagcat ttggtaagct taaactatcg aatatctctg tcccatattg
     8461 tcaaaaagtg gtagatgaat ggcacagtaa atatgaaagt tacgattata tgagaaaaca
     8521 gactgcacaa attttacgct acggggttgc aatgcaatat attgacaata atcctatgga
     8581 aaaaacactt cttccacgaa aaaaagagta tgagaaaaat cgtaaatttt actcaaaaga
     8641 ggagcttaat aatttactgg atgcttttaa agattttggt aacatgaaac aatacgcctt
     8701 cttcagatta ttggcataca caggtatgag aaagagtgaa gtactcgctt tacagtggaa
     8761 agacatagac acgtttaata aagaactaca tgtcaataaa actcttgctg tagatgaatt
     8821 tggtaaagtt ataattcaat ctccgaaaac aagagcttct agacgagtta tctctttaga
     8881 tactgagact ctttctatcc tcaacaactg gaaattacag caaaaagaag agtatttaaa
     8941 acttggatac aatactagct ctaaagagca acatgttttt acaacagtta aaaacactct
     9001 atacattcca aacactgtta atgattggtt aagatatatt ttgaaaaaat ataacttgcc
     9061 tcgtattaca cctcatgggt ttagacatac acatgctagt ttattattag aagcaggaga
     9121 atctgtcaag gtagttcaac aacgattagg gcatgaaaat agtaaagtga cattagacat
     9181 ctatgcacat attacaaata atgcacctaa gaaaacaggt caagactttg ctgatatgat
     9241 ggctcatcag taaaagagct ggtcaaaatc tggtcatttc tagtatacag gcaaaacaaa
     9301 aaagctagaa cccttatata tcaagggttc tagctcctat aacaggttta cccaacactg
     9361 ccttccaatg actcttttgg gaatcagagg aaagaatcag gctttataga gatatttcat
     9421 ctaacaaatt actttagact gacagtatat atcagcaggt actgtcatcg ggaacgtagc
     9481 caatatcgta tccaaaacag caggtagaga agctatttga ggattcaagt tattctattt
     9541 caaccatgaa gttaagttca ttaaaaataa agatagttgg tatttcaata atttttgtat
     9601 agagttaatc actttattga tgctaattca tcttgattgg tattataatc gtattcaagt
     9661 tg
//
LOCUS       DAYYNG010000111         9574 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000111 DAYYNG010000000
VERSION     DAYYNG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.111"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..1137
                     /locus_tag="ACHBFA_002099"
     CDS             139..1137
                     /locus_tag="ACHBFA_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9395542.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1249..1758
                     /locus_tag="ACHBFA_002100"
     CDS             1249..1758
                     /locus_tag="ACHBFA_002100"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM9395543.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1788..2447)
                     /locus_tag="ACHBFA_002101"
     CDS             complement(1788..2447)
                     /locus_tag="ACHBFA_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9395544.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2449..3213)
                     /locus_tag="ACHBFA_002102"
     CDS             complement(2449..3213)
                     /locus_tag="ACHBFA_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9395545.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3221..3898)
                     /locus_tag="ACHBFA_002103"
     CDS             complement(3221..3898)
                     /locus_tag="ACHBFA_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9395546.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3935..5320)
                     /locus_tag="ACHBFA_002104"
     CDS             complement(3935..5320)
                     /locus_tag="ACHBFA_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM9395547.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5530..5614)
                     /gene="ffs"
                     /locus_tag="ACHBFA_002105"
     ncRNA           complement(5530..5614)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHBFA_002105"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5637..6152)
                     /gene="tadA"
                     /locus_tag="ACHBFA_002106"
     CDS             complement(5637..6152)
                     /gene="tadA"
                     /locus_tag="ACHBFA_002106"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9395548.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6259..7116
                     /locus_tag="ACHBFA_002107"
     CDS             6259..7116
                     /locus_tag="ACHBFA_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395549.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7197..7532)
                     /locus_tag="ACHBFA_002108"
     CDS             complement(7197..7532)
                     /locus_tag="ACHBFA_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395550.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7599..8969)
                     /locus_tag="ACHBFA_002109"
     CDS             complement(7599..8969)
                     /locus_tag="ACHBFA_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9395551.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9047..>9574)
                     /locus_tag="ACHBFA_002110"
     CDS             complement(9047..>9574)
                     /locus_tag="ACHBFA_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9395552.1"
                     /translation="RVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPIYT
                     HFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDL
                     DMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNE
                     VTDEYLENILKEQSP"
BASE COUNT         2801 a         1969 c         1618 g         3186 t
ORIGIN      
        1 aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta taacttaccg
       61 tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct gctatagtgt
      121 gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc aggtcctgta
      181 gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat cattgacagt
      241 ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat ttacgatatt
      301 cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa acaattaact
      361 acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg tgaagacgaa
      421 gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat tttagcttta
      481 ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc atttgaaaac
      541 cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt
      601 gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat tgcaagcgaa
      661 gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt atcacgcttg
      721 aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc aggcaataat
      781 ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg
      841 attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct ttcttcttgg
      901 ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac ttctattcca
      961 ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact gattgctact
     1021 gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa tccaaaggaa
     1081 cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc tagttgattt
     1141 tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt
     1201 tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa
     1261 actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa
     1321 gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt gactttggcg
     1381 gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa cgccattatc
     1441 actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc gttgcaacat
     1501 attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt gtcaatcgtg
     1561 aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt gaaacctggt
     1621 attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt agatgatatc
     1681 gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc agaaaaaaga
     1741 agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta gacgagttct
     1801 ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc aaaaaataat
     1861 tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg aagtacattg
     1921 atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga
     1981 aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac aactggcagt
     2041 aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt ttgcaaatat
     2101 ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc cactaaaaac
     2161 aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa atctggcaag
     2221 ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa atttttcagt
     2281 aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat atcaaatacg
     2341 tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt cagaaatagg
     2401 ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt agaagctcca
     2461 ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta catcttcttc
     2521 ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc cagcttggat
     2581 atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc ctaatacttc
     2641 aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg gcgtttgggt
     2701 tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc gttcagtagg
     2761 tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag caagtgttgc
     2821 tttaactact ttttcatagg tcagataatt atctaagcct accactacat agtcaggagc
     2881 ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg cttctccgat
     2941 aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg ccaaagtagc
     3001 tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac gattagctac
     3061 tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt ttcttttttg
     3121 caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat aaatcgttcc
     3181 gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc ctttttctga
     3241 tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt tttacttttt
     3301 cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg cgattagatg
     3361 cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc ttttttcggc
     3421 gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac gcacaaccaa
     3481 aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct ggatgcgccc
     3541 gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc caatctccta
     3601 cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt tctgcattaa
     3661 atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa aaattactgt
     3721 catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc tcaacgattt
     3781 cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct gatgattttt
     3841 tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt gcagacatgc
     3901 gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag tgagctttca
     3961 agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt tttccagcaa
     4021 tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta aaagtataat
     4081 ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata gaatcatatg
     4141 tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag
     4201 gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg atcagatcat
     4261 ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg tgaagctgat
     4321 cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt aatattgctg
     4381 cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac gaatgctcat
     4441 tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct tttaataatt
     4501 catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa gctgtcattg
     4561 tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca tcgatggtta
     4621 aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg acatcattga
     4681 ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca tcaattgctg
     4741 gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc aaaactaaaa
     4801 catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa ggattccgta
     4861 tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca attagtccga
     4921 ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc ggtggctgga
     4981 gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga tcgtataaat
     5041 gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc gctgcatcat
     5101 aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct gacaatggat
     5161 gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt tctttctttt
     5221 cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt tctaaatgag
     5281 aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc gcctctcttt
     5341 atctttatca taccataggc agctttttct tccattgaaa caagctctat ttcagagttt
     5401 ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct ggctaaaaaa
     5461 tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg aaagtctgtt
     5521 gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc cttccagacc
     5581 tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag tataccttat
     5641 ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag ctttcggaaa
     5701 aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac atatgcttgg
     5761 tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc gccttttgga
     5821 tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata
     5881 ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct tcccactttt
     5941 tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg cgtggtttct
     6001 ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc tccaatcgga
     6061 acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat ccatttttct
     6121 ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt gttttttctt
     6181 gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat ggtaaaataa
     6241 atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc acaagcacag
     6301 aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga tgatgctttt
     6361 atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa agcgttattc
     6421 cctgtaatca acgatcaaaa aaaacaccca tggtatcgct atctttttaa agatgcttct
     6481 tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc
     6541 ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc tgatttcttt
     6601 ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta tcttgatggt
     6661 actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac aacgtctgta
     6721 ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga
     6781 aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt ttctatgcaa
     6841 gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt gatcaaaagt
     6901 tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa
     6961 ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat
     7021 cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa cgatttactg
     7081 ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg ggttgtgaca
     7141 ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag tttctcttac
     7201 atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga atctttgatt
     7261 gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa cattcccatc
     7321 aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt ttcatttcca
     7381 tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg atggatcttt
     7441 tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc tgctacgctt
     7501 aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt atctatctcc
     7561 atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct caatccttta
     7621 gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg tatcttatct
     7681 aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa
     7741 gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt ttttgtagca
     7801 agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa atgcaaacct
     7861 gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc ccaaagatgg
     7921 tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga aaactccgtc
     7981 cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt cttcttttct
     8041 ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga
     8101 tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc ccattcagaa
     8161 cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg ataattttct
     8221 acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt cgaataaatc
     8281 agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg ctgacgtttt
     8341 tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc ttttcggaac
     8401 attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc cgaaaaatga
     8461 gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc ccatcgttct
     8521 atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac taataaccct
     8581 ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc acacaaatcc
     8641 aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc acttggttct
     8701 tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt gccaagaaat
     8761 ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc
     8821 aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc ttgttcaagc
     8881 gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt gataaattgc
     8941 tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt ttacaatctt
     9001 ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa ggtgactgtt
     9061 cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa acagcgttca
     9121 tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat gcattaagcc
     9181 ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca tatgagaaaa
     9241 atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt tcacgaatac
     9301 tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca tcatcggaac
     9361 tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc ttctctttta
     9421 acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg cagaactctg
     9481 ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc atacttttga
     9541 acagcaaata ttctttacta taatttgtta ctct
//
LOCUS       DAYYNG010000112         9550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000112 DAYYNG010000000
VERSION     DAYYNG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.112"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..2101
                     /locus_tag="ACHBFA_002111"
     CDS             218..2101
                     /locus_tag="ACHBFA_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9395553.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            2113..3060
                     /locus_tag="ACHBFA_002112"
     CDS             2113..3060
                     /locus_tag="ACHBFA_002112"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9395554.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3080..3607
                     /locus_tag="ACHBFA_002113"
     CDS             3080..3607
                     /locus_tag="ACHBFA_002113"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9395555.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3670..4323)
                     /locus_tag="ACHBFA_002114"
     CDS             complement(3670..4323)
                     /locus_tag="ACHBFA_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9395556.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4455..5297
                     /locus_tag="ACHBFA_002115"
     CDS             4455..5297
                     /locus_tag="ACHBFA_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9395557.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5455..6351
                     /locus_tag="ACHBFA_002116"
     CDS             5455..6351
                     /locus_tag="ACHBFA_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9395558.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6354..7067
                     /locus_tag="ACHBFA_002117"
     CDS             6354..7067
                     /locus_tag="ACHBFA_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM9395559.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7087..7605
                     /gene="msrA"
                     /locus_tag="ACHBFA_002118"
     CDS             7087..7605
                     /gene="msrA"
                     /locus_tag="ACHBFA_002118"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9395560.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7602..7829
                     /locus_tag="ACHBFA_002119"
     CDS             7602..7829
                     /locus_tag="ACHBFA_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9395561.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7981..8532
                     /gene="lepB"
                     /locus_tag="ACHBFA_002120"
     CDS             7981..8532
                     /gene="lepB"
                     /locus_tag="ACHBFA_002120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9395562.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8615..9157)
                     /locus_tag="ACHBFA_002121"
     CDS             complement(8615..9157)
                     /locus_tag="ACHBFA_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9395563.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         3437 a         1660 c         1896 g         2557 t
ORIGIN      
        1 ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg agtccaataa aataaaaagg
       61 taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga atcggtcctg
      121 ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga agttcactat
      181 ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa aagtaactga
      241 cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt tggtccacga
      301 aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc tcttaaatat
      361 tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc caactgatta
      421 ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg ttctccaagc
      481 agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg aagcattgat
      541 cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta aagcagaaga
      601 acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta ttttgcaaaa
      661 attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc agaaaaaacg
      721 agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag acgaaccaac
      781 aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac aataccgtgg
      841 cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa accgaatctt
      901 cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt acgtcttaga
      961 aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc ggctttataa
     1021 acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc agcaagcacg
     1081 gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag aagatgattt
     1141 agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca aagatggcag
     1201 ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc aatctcggga
     1261 aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta atattctagc
     1321 tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc atcttgcgta
     1381 ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt acttacaaga
     1441 agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag aattacttga
     1501 acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt caggcggaga
     1561 aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt tattattgga
     1621 tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt attttagaag
     1681 ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca aggtagcaga
     1741 taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa acatgtccag
     1801 ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac caaaaataca
     1861 gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag aatgggaaac
     1921 gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag aagagatgaa
     1981 tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat ctgaaacaga
     2041 agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg tagaagatta
     2101 ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg
     2161 catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata ccaaatgcgg
     2221 tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt tggattgatc
     2281 aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct attacaaaat
     2341 aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc agactataat
     2401 ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt caatgaagaa
     2461 tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc ctttgctgaa
     2521 acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga aacaaaagac
     2581 ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac tcctgattct
     2641 agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc tttgcctcct
     2701 tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca attgtatcaa
     2761 cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc gttgctgact
     2821 cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac attaggagat
     2881 gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg agaaatacgc
     2941 gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt tgagatggaa
     3001 gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat cgctgtttag
     3061 aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa acggattgat
     3121 tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct tcaaacaaat
     3181 gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg gtagccgtcc
     3241 gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag cagaaggcgt
     3301 gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag acggtatctt
     3361 tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta cgatattata
     3421 ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg ttgactggtc
     3481 tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc gttatccgca
     3541 tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac
     3601 gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc catagcttta
     3661 ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac ctaacataac
     3721 aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa agaatgcgcc
     3781 tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc caagagagtg
     3841 ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga tgatcgtaga
     3901 tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa tgccaagaat
     3961 cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga tactcagcaa
     4021 acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg
     4081 gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa
     4141 aagaatcatc atcgatccat agatagcata agagacaata tggagggagg aatgcaagtg
     4201 ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc caattccatg
     4261 ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt tcgttttttc
     4321 catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg tctgttatac
     4381 taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg ttaaagaaag
     4441 agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt acgatggaaa
     4501 aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg atcgacggaa
     4561 cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg atggcgaaag
     4621 caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg atgtatgatg
     4681 agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc ttgagtggaa
     4741 cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg gttatcgaca
     4801 gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa ttagcaaaag
     4861 aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac aatacaaaat
     4921 tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc agccgaacaa
     4981 ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc cattctgaat
     5041 taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt aataatttga
     5101 aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat gcggatgcaa
     5161 aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac atgcatatcc
     5221 ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg tttgcaatca
     5281 tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc ttgcagctta
     5341 ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata aagactacct
     5401 ttttgataaa atttttgata acattagaac caatcgaata caagaggtga aaaaatgaaa
     5461 ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat catcatctat
     5521 gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt aactactgtg
     5581 agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga ttcgctgact
     5641 gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc aaatgatcta
     5701 aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa tggtgaacga
     5761 agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa tatcaaaaca
     5821 gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat ccaaaataat
     5881 ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca ggaaaatgtc
     5941 acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta cattttaggc
     6001 atctacaatc cattttattt gaactttcct gagatcactg acatgcagac aattgttaac
     6061 aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta ctttatccca
     6121 atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga agaaggaaca
     6181 gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt gctctatgaa
     6241 gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc agtgagagat
     6301 cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata aacatgaaaa
     6361 caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa ttcgccttcc
     6421 ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt atttttgcta
     6481 atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga gatcctgtct
     6541 taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc cttagtgagt
     6601 atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca ctactcaatg
     6661 gtactttcga agtactaggt tttcctatcg atttctattt atattttgat ccgtatgtga
     6721 tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca ttgagtttgc
     6781 caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat tggatcgaag
     6841 tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg caaaacggga
     6901 tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt agtctatatt
     6961 tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact agcaccagca
     7021 gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat tagaggggga
     7081 atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat ccagcctttc
     7141 gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca tgtaccagat
     7201 ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt ggagattatc
     7261 tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca gcaaactgat
     7321 ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc ggtgatcttt
     7381 tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt agctgaaagt
     7441 ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt ttatctagca
     7501 gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag aaaccatgca
     7561 cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga agcttttttc
     7621 attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt ttcgctacag
     7681 aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa atttctaatt
     7741 atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa gcatgggaaa
     7801 aatatttaga aaataatcat gacatataag agatagtttt gtactttatg atcaaaaaag
     7861 ttttttgtcc aatagcctaa ttgaaagaat tatttttatt tcagctatct agtcgcattt
     7921 ttttgatatt tttcttataa taaagaaaga atgaaaaaaa gagggatgga ttgatagaga
     7981 ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg tattcaaata cttacttgtt
     8041 tctgtattcc ttgcgttcat gttgcgggga tttttattca tcccggtacc agtggaaggc
     8101 aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa attttcggag
     8161 atcagacgat ttgatattgt tgtttttcaa ttagcagatg gcacgattta tatcaaacga
     8221 gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg atcaattgaa gatcaatggg
     8281 aaagtagtta aggaacctta tctaactaaa aacctgaaat cagatcatgc aaatgcatcc
     8341 tatacgacgg actttacact acaagagcta acgggacaat ccaagcttcc ggaagataat
     8401 tattttgtac ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt tggcacgatc
     8461 aataaaactg atattctagg taaagcaaga tttgtctatt atccacttga tgaaataaaa
     8521 tggatccagt gagaaacaat aaagcgtgca gtctgttgag actgcacgct ttattgttta
     8581 atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta aacacttttc
     8641 tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg atttggatca
     8701 tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac aaaacagaca
     8761 tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg gcagcgatga
     8821 cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat ccaccaagga
     8881 aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg aaatttaaag
     8941 tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa cccataagcg
     9001 ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct atcttgtata
     9061 agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca ttcatttcgg
     9121 gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct ttgtcagtta
     9181 ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga tttacataca
     9241 ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaactg taaaaaagag
     9301 attgtacgaa taaaaaaaat agaggcggag aatacttatc gtatcatccg cctttgtttt
     9361 tttgttataa aaaacgtcag attagttatc tgcttacatc ttcatccata cgggttctaa
     9421 tgtatttccg tctagatctt ggacttctaa tgaaaacatt tcttcttcag gcattcccat
     9481 atctacttcg tggaaagtac caccattttc ttttgcaatt tgagcaaatt tttttacttc
     9541 atccgcattt
//
LOCUS       DAYYNG010000113         9407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000113 DAYYNG010000000
VERSION     DAYYNG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.113"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..240
                     /locus_tag="ACHBFA_002122"
     CDS             <1..240
                     /locus_tag="ACHBFA_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9395564.1"
                     /translation="QFLPVKDKNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQ
                     QVLINNYEDVLLDQSRKLRLRPFESIVLANYGGQL"
     gene            237..1697
                     /gene="gtfA"
                     /locus_tag="ACHBFA_002123"
     CDS             237..1697
                     /gene="gtfA"
                     /locus_tag="ACHBFA_002123"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM9395565.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            1774..2880
                     /locus_tag="ACHBFA_002124"
                     /pseudo
     CDS             1774..2880
                     /locus_tag="ACHBFA_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            3202..3411
                     /locus_tag="ACHBFA_002125"
     CDS             3202..3411
                     /locus_tag="ACHBFA_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9395566.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            3404..3553
                     /locus_tag="ACHBFA_002126"
     CDS             3404..3553
                     /locus_tag="ACHBFA_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM9395567.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            3707..4372
                     /locus_tag="ACHBFA_002127"
     CDS             3707..4372
                     /locus_tag="ACHBFA_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9395568.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(4656..5741)
                     /gene="xerS"
                     /locus_tag="ACHBFA_002128"
     CDS             complement(4656..5741)
                     /gene="xerS"
                     /locus_tag="ACHBFA_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9395569.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            6075..6308
                     /locus_tag="ACHBFA_002129"
     CDS             6075..6308
                     /locus_tag="ACHBFA_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM9395570.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            6305..6712
                     /locus_tag="ACHBFA_002130"
     CDS             6305..6712
                     /locus_tag="ACHBFA_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9395571.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(7849..8367)
                     /locus_tag="ACHBFA_002131"
     CDS             complement(7849..8367)
                     /locus_tag="ACHBFA_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM9395572.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            8734..8982
                     /locus_tag="ACHBFA_002132"
     CDS             8734..8982
                     /locus_tag="ACHBFA_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395573.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         3233 a         1486 c         1596 g         3092 t
ORIGIN      
        1 caatttctac cagtgaaaga taaaaatgaa catattttgc tttatgaacg ccaattagaa
       61 gataaaatcg tattagttat cttaaacgtt acagcaacag aacaagaata ttatgtagat
      121 gaacagtacc aacaagtatt aatcaataat tatgaagatg ttttattaga tcaaagtcga
      181 aaattgcgtt tacgaccgtt tgaaagtatt gtattagcta attatggagg acaattatga
      241 aaattaaaaa cgaagcaatg ctcatcacct atgcagatag tttaggaaaa aacatgggcg
      301 aattaaaaca agtcttagac aaacatttac aaggtgttat tggtggtgta catctgttgc
      361 ctttttttcc atcgacaggt gatcgaggct ttgcgccgag tgattatacg acagttgatc
      421 cagctcttgg aacatgggaa gatgtggaag cattaggtga agattattat ttgatgtttg
      481 attttatgat taatcacatt tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc
      541 atgagcagtc accatacaaa gaaatgttta tccgtattca tgaatttttc ccagaaaatc
      601 gaccaacaca agaagatatt gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa
      661 cggtagaatt tgcggatggt acgacagaag aggtttggaa tacctttggg gaagaacaaa
      721 ttgatttaga tgtgaccaaa gaagttgtga aagaatttat tcgagaaacc atcaaagata
      781 tggcgaacca tggctgttct ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat
      841 tagataccaa tgatttcttc gtggaaccag atatttggga acttttagac gaagtgcgtc
      901 aagaagcagc gaaatatgac gtcgaattat taccagaaat ccatgagcat tattccattc
      961 aaatgaaaat tgccaatcat gattattata tctatgactt cgccttgcca atggtcactc
     1021 tgtattcact ttatagtgga aaatcagaac gtttagccaa ttggttgaaa atgagtccga
     1081 tgaagcagtt tacaacattg gatacgcatg atggaattgg tgttgtggac gctcgtgatt
     1141 tattaacaga tgaagaattg gattacacct ctgaggaatt gtataaagtc ggggcgaatg
     1201 tgaagaaggt ctattcaagt gccaattaca ataatttaga tatttatcaa attaacagca
     1261 cctattatag tgcattaggc gataatgacc gtagctatct gttagcacgt gcgattcaag
     1321 tatttgcgcc agggattcca caaatttatt acgttgggtt attagcagga aaaaatgata
     1381 ttgaactgtt ggaagcaacg aaagaagggc gtaatattaa tcgtcattac tatgatttag
     1441 ccgaaattga agaagaagtg caacggccag tgattcaaca attatttgca cttttaaaat
     1501 ttagaaatga atctgcggcg tttgatttgg atggaacgat tgaagtagca acgccatcag
     1561 aatcagaaat agtgattaca cgtaaaaata aaaacggtag tgatagtgca acattaacgg
     1621 ctaacttgga tacgaaagaa tttatcattg agtctaaaga tataaaaatt ttcactagtg
     1681 ttgcattaaa attttaacct attccataga acaaaaaatc ctttatacta gcacttggat
     1741 gacttatcca agaccaataa aaaggactca tacatggata agtataaaac aaatacagca
     1801 tttaataaat ggttttctgc cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt
     1861 atttttgatt acaattcgta tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt
     1921 ctctacgcag ttaatgaaga aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac
     1981 cgatcgctcc aactagaaac cgatgtgtca tcgattagtc atacccaatt gtctcgagca
     2041 tttaatgcac ttgaacctaa agtgttagaa gaaatttttc aacaactgtt agaaaaagtt
     2101 caacatcaaa tcaaaccaac caagcgaaat catctgtatc tgattgattc cagtacgttt
     2161 tcgcttagtt taaaacgtca taagtgggcg actttccgtc aaacaaaatc aggtgtcaag
     2221 ttacacttaa actattgtta tatggacgat actacgatgt atccaactga ttttacctta
     2281 acaaatgccc atgaacatga tctcaatcaa ctaccagtat tggtgaatca accagaggca
     2341 acttacgtgt ttgatcgtgg gtattaggac tttgaaaaga tggatcagat gcattgggat
     2401 ggctatttct tcgtcactcg gattaaaaaa aacaccaaag ttcacgtgat agatactctg
     2461 gaaacatcgc cagaaacaga aattctacga gacgagctgg ttcgtttagg atctaaaacc
     2521 tatctaaccg ccaatttccg actagtcact gttcaagata agaacggacg tgtgtttcaa
     2581 tttatcacca atcgaatgga cgtctcttca aaagaaatct ctgatatgta tcacgctcgt
     2641 tggcaaatcg aattgttttt caagcacatc aaacaacaca tgaccattaa aaccttcttt
     2701 tctcaaagtg aaaaaggcgt gcagaatcaa ttgattttaa cgatgatttc cgctctcctc
     2761 acgtttctca tcaaattgga gacgaaaacg gaaaaatcag tgttccaaat caaacgattc
     2821 tttcgttatt tactgtttca gccctttgaa tgttgtctag aaaaattaat tcccacatga
     2881 gaaaaaagaa atttggctcg ttacttttgg ttttcagttg aataacagat aaaaaatttt
     2941 ctggaaaaag tcatcctctg cgtcagcaca gttttttttg acttattttc agaggaaata
     3001 gttttcagaa tattctaaaa tgttttatgc aacactagtg atatttttta gaattagatt
     3061 tctaatgtgc attttttgag tattaaggag caagtataca aagatttata aagctgatta
     3121 aatttgaata aaaatatgca ggaacgctac tatttttagt agtgttcttt tttatatttg
     3181 aataaaaaat gtggtggtta aatggtggtt ataacaaaaa atgaattaat gaaattaggt
     3241 tatgggaagt atcagtctga gagaatcata cgacaggcaa aagcaattat ggtttcaaaa
     3301 agctatacct attacgaaaa tagaagatta ggactagtac ctgttgaatc tgtagaagaa
     3361 gttttaggta tgaagataga ggtagaggag gattttcaga atcatggtta attttaaaaa
     3421 agataaaaat gggaaatatc attttactgc tagtttggga ttcgacaaac gaacagggaa
     3481 aagagtacaa aaaaacgtag aggggttaaa acagttaaag aagaaaaaaa gcgtataccg
     3541 aacttgtttc taattatgga aaagaagcag aaacttctca aagtactatg ttgtttgagg
     3601 agttttttta taaattattc ctaccttatt atcaagggaa agtagaagaa agaacatata
     3661 aaaatcggtt atctacaatc aaaaaaactt ttcttatttc tttaaaatgg agttaagaga
     3721 aatacgtcca attgacattc aaaagtggca aaataattta ttgagcaaat ataaaaattc
     3781 ttatgtaaga aacatgtatg ggatttttca gatgtcatta gatcgagcag ttgttttggg
     3841 actacttgaa aaaaatcctg caaaagttat tggaaatgtg aagaaaagga aaactacagt
     3901 tgatttttgg acaaaagaag aatttacaaa agttatttca actttttata ttgatgattt
     3961 gtaccaacat tttggtttta tttgtatgtg gttacttttt atgaccgtta tgcgtttggg
     4021 agaagccact gcattaactt ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa
     4081 taaaacactc tattataaga gagccaaaac ctatgagttt gtagaacccg aaacggcttc
     4141 tagtattcgt acaatcgaac tagacgacaa tacaattacc tttttaaaag cttggaagga
     4201 gcgtcaagtt tcaataggag gcatgtcgtt tgtactttct tataatggaa cacctactca
     4261 gaaatataca atccgtcata ttatgagaag acatgcaacg atcgttggtg ttcatataat
     4321 ccgcatacac ggattaaggc atagtcatgc ttctttgctt atatcacaat aaaaagatgc
     4381 acttcgaatt aaagaaaggt taaaacatgc tgatgtacaa acgactttag gaacttatgg
     4441 tcatttgtat ctaaattcat tgagagagat aacaaatgat cccacagatt atatccaaac
     4501 taatttttct aaaatcaaac cgacacctat ttacgataat aaaaaataaa tgataaacaa
     4561 aagtaggatc acaccaaaag aaacacacta gaaaaactat aatacaggct tttctagcgt
     4621 gttgtttctt atttccgcct atttatacat tatactcata aagcatttaa tgcatcgatc
     4681 gtagccgcat tgtctatatg ggtatataaa gttgttgtat tggtacttga atgtcccaat
     4741 tgttgggcag ttaataccaa tgaatttgtc tgttggtaga gtctagtagc taatgtatga
     4801 cgcagtttgt gagggctgac ttgaattcta aagccttttg aatattttgc taccatccgt
     4861 tcgattgcaa taccggatat acgcttggct tctccttggt aaattgctag aaataaagct
     4921 ttttcgtttt ctgttacttt atatcgttct ttccgaatag ctagataatt agctaaatat
     4981 tccattgcga atgctgctat attgacagca tctcttttat ttccttttct agttactacc
     5041 acattattct tatctaaaga taggtcatct aaattaatat ttataacctc ggagagtctc
     5101 aatccagatc ctaaaatcag tgcattgatc gccaggtctc tttctacatc tcgttgataa
     5161 tagatcaaag ctcgtttact caaagtattt ttatattcat tggcaatata atctagataa
     5221 ttttgtgtat ccttatctag aaataatttt ttctcaatgg cattggcacg tgcagctaac
     5281 gtagttttat ctgtgatggt tgctacttga tccataggat tattgtctaa gtatggtttt
     5341 cctagaggat tattggaact tttcgaaaga tatttaaata gaacctttaa tgccgtgata
     5401 tttctattga tagtagaagt agatagacct aagttattat tatctttgag cttagttttt
     5461 gttgtctctt tttttacatt ttcttttgtg cgacgtttca aatgagattt gtacaagttt
     5521 aggtcatagg cagttaactg tcttatcgag gtgatagaag tatcaggaac gatagactca
     5581 ctcagaagcc attcataaaa gagcttataa ttttttagat attctaaagt ggttcgaggt
     5641 gattgatctt tatcctcacg atcttgaaca aaatctttta tataatcggg catatgaagt
     5701 atgattgtat ctattttttg atatatttct gtatttttca tgtcattcat cctttcaata
     5761 agtaggggag aagagtatct ctataattgg actataccaa ttatagaatg ttttgtcaat
     5821 tggtatgctc taaaatttat taatttgttt caaaaaaagc atataaacag gtatacgttt
     5881 aataatttaa aataaatggt atacataaat agggaaggaa atagaaggga gcaatctaaa
     5941 ttttttgaat tttcttaatt aattatatag aaagaacaat ctttctatag agttagacat
     6001 gagagggact atgcttgatt taaaatcaac aagcgtatat actgtatata caaagaaaaa
     6061 ggagggagaa gactatggca attaaagaaa gaaaatttag ggaagtgggg aactcagtag
     6121 tattaactat ttcaaaggag tttcttaaag aaaatgggtt aaatcccgga gatacggttt
     6181 ttttagatga agaaaaactt cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa
     6241 ttgatttagc aatatctcaa gcattaactg aatatgatac tacttttaga gagcttgtcg
     6301 ataaatgagt attcattacc tagaagccaa agatattata aaaatgaatg taatccaaat
     6361 aaaaaaatac tctcctaatg aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg
     6421 tgtagggcaa cttcgagcct cagcttttgg tgaagaggtt tatccttcga tttatgaaaa
     6481 agcagctatc ctacttatac aattgataaa gaaacatcct tttcacaatg caaataaacg
     6541 tactgctttc ttagctactt ttgtcttttt gaaaatcaat ggacagcttt tagctattga
     6601 tcaaaaagat gctgtaaatt tagcggtata tattgctacg tatgataagg atttcgatca
     6661 actaaaatat gaagtgatca acactataaa gacacatacg cagcccattt aaggagttac
     6721 tgtatgtgtc tttatagggt tattagctgc tatagcgaaa taattgaagt aaattacaaa
     6781 tagctataat tcaaatccaa ctatatagaa aacgtaaatt ttctatatag ttggattaag
     6841 ataacgacta tttattttat catattttta ctatacccta tacttttact tggtatattt
     6901 atgaataatc gcaaatttac ccctatacta ctagtttgta agttagatta ataaaaatac
     6961 taaagttatt tgttctttta ttaaatacac ctacaactta taaattgatg gtatttagat
     7021 aacacgaaag aataaaaata acagctgata gtaaagtttg ttgacgattg ctactcaatc
     7081 gaaagtgagt tagtgcgtat gaacttgctt ctgaaaactt atgaaatgaa agacctttta
     7141 catcttgcag aaatcggtac tgggattaaa ctaagcaata aaaactaatg ttcccctcat
     7201 tctttgaact tctactattt gatttattta ttctgataaa tgacgatttc accttcagga
     7261 tcttcaaatc ccgtatagaa tttatttaag atctcttcag tatcagtaat aggataggcg
     7321 ataggccaca taccctttga tggttagaat aaactgctca aaatctgtat cgtatgttgg
     7381 ctttagcata ttaacaaagg ctatctccaa attataaacc ataagcgttg aatcgttctt
     7441 aacaatttca attcttccta acccaaagtc agaagatata attaccggac ttgccttacc
     7501 cttcatgtgg gttgttgaga ttccatattt aaagggaatt gtcggtacta tcggcggtct
     7561 gaaaaacatc ccaaaaataa aaaaatcttt cccctgctag tctcctaagt ccctttgtag
     7621 cggatatggc accataatct cattttatcg aagtgagtca tggtttatct gttggccaac
     7681 caaaattgat acaatctaac gatgcaccct ctacggcatt ctaggggagt gcacttgcat
     7741 tcacccaata aaaaccggca gttagcaaag aacttatgta gtcgtatgtt cttactgtta
     7801 acatacaaga ggtctccttt ccacaattgc ttagcagtaa acgggaattt acaaagctaa
     7861 tctcttccat tttcttatct ttgttctaaa agtcccaaat ggagcaactg tatttacatg
     7921 aataaattta tatacttccc atacagccgt tttggttgcc tcgtctgccc attttctttt
     7981 gtgtgcagtg aatagatctt catcactcat tgaatcaatc atcatgttaa tatcatcaat
     8041 attacgattg agaatttcct ctaattcttt caatggtaga tgtgcatagg tatttgtaaa
     8101 ccacttatat aactctccta actgattcca tttaaattga tcagaaggcg ttttaacaga
     8161 taatccattt ctttcatcat tttcccattt tagaattaaa ttcgtccaac caacttgata
     8221 tgctaaattt tcagcaggtg ttctatccac ttcctcaact cttttatcct tcaattcttc
     8281 aggaatagtt tcaaattcta aaatatattt agtataagtc ctatttattt ctttttttaa
     8341 ttcttcttta tttttatatg ttctcattat attacctcca taaattccta attgtctcct
     8401 ttaattcatc tataatttca tccacgatat tttaatccgt tctacaatgc atatctgata
     8461 tgcattgact gtctaactca tatgtgaatg atttataaaa tatcccaacg tctcatctaa
     8521 agaaatgttg atcgtcagca aggagctaaa aaatacgaat atatttcttt tgtagtaaaa
     8581 tcggactggt ttgcatatca ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa
     8641 ttactaataa aaactaccac tttaagcgtt aagaactgtt aagtttagtt aagtgaggat
     8701 agttcacaaa atccattgtt aaacttcacg aaagtggtaa aactcaaaat gctttatata
     8761 ggattacagc atcgctcttt cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa
     8821 attgacgatg acacgttcct tacggctcat cagatgaaac agctacaaaa acgaaatgat
     8881 caactcgaag aaaatttaat tctaaaaaag cgattgccat attcacgcct cgctcgacga
     8941 actattaaag gtcaagattt acctgaagat catccttttt aggaaattga agctttttat
     9001 gttgcacaat gcatttataa tactacaatg atgttttcac cagatattct tctagggtgc
     9061 ggtgtaatga aacagataca cttgcaaaag aaaattcacc gcgaatttac taaaattatg
     9121 aataattatg ttaatcttcc gaaatttttt tccaaaatta agggatcaag ctggtgttat
     9181 agattgttta aggttggcaa aaaaataact tctttttaaa caattataga aaaccgatac
     9241 tctgttaagt tggttgacac ggtgtcgatt tttatgactt tcttattctg aaacttcact
     9301 aacggacagt acctaaaaag tctatagtat ggacgtttac gagtactatt ttatcaaaaa
     9361 tgctgctcat ttcaacttat atgttttagt gaactacccc cacttag
//
LOCUS       DAYYNG010000114         9273 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000114 DAYYNG010000000
VERSION     DAYYNG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.114"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHBFA_002133"
     CDS             <1..435
                     /locus_tag="ACHBFA_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9395574.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHBFA_002134"
     tRNA            complement(759..842)
                     /locus_tag="ACHBFA_002134"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHBFA_002135"
     CDS             1370..1870
                     /locus_tag="ACHBFA_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9395575.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHBFA_002136"
     CDS             complement(2060..2404)
                     /locus_tag="ACHBFA_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9395576.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHBFA_002137"
     CDS             complement(2558..3478)
                     /locus_tag="ACHBFA_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9395577.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHBFA_002138"
     CDS             3665..4156
                     /locus_tag="ACHBFA_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM9395578.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHBFA_002139"
     CDS             complement(4180..6555)
                     /locus_tag="ACHBFA_002139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM9395579.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHBFA_002140"
     CDS             6881..7507
                     /locus_tag="ACHBFA_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9395580.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHBFA_002141"
     CDS             7530..8234
                     /locus_tag="ACHBFA_002141"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9395581.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHBFA_002142"
     CDS             8426..9259
                     /locus_tag="ACHBFA_002142"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9395582.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1788 c         1651 g         3006 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga aca
//
LOCUS       DAYYNG010000115         9250 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000115 DAYYNG010000000
VERSION     DAYYNG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.115"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..650)
                     /locus_tag="ACHBFA_002143"
     CDS             complement(<1..650)
                     /locus_tag="ACHBFA_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9395583.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPL"
     gene            784..1071
                     /locus_tag="ACHBFA_002144"
     CDS             784..1071
                     /locus_tag="ACHBFA_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395584.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1487..3025
                     /locus_tag="ACHBFA_002145"
     CDS             1487..3025
                     /locus_tag="ACHBFA_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9395585.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3022..3987
                     /locus_tag="ACHBFA_002146"
     CDS             3022..3987
                     /locus_tag="ACHBFA_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395586.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3974..5296
                     /locus_tag="ACHBFA_002147"
     CDS             3974..5296
                     /locus_tag="ACHBFA_002147"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9395587.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5359..5463
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5537..6280
                     /locus_tag="ACHBFA_002148"
     CDS             5537..6280
                     /locus_tag="ACHBFA_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395588.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6295..7287
                     /locus_tag="ACHBFA_002149"
     CDS             6295..7287
                     /locus_tag="ACHBFA_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9395589.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7299..8036
                     /locus_tag="ACHBFA_002150"
     CDS             7299..8036
                     /locus_tag="ACHBFA_002150"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395590.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8026..8747
                     /locus_tag="ACHBFA_002151"
                     /pseudo
     CDS             8026..8747
                     /locus_tag="ACHBFA_002151"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
     gene            complement(8831..>9250)
                     /locus_tag="ACHBFA_002152"
     CDS             complement(8831..>9250)
                     /locus_tag="ACHBFA_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9395591.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3025 a         1598 c         1957 g         2670 t
ORIGIN      
        1 agaggatcaa gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca
       61 gatccaattt ccagatactt cactttattc aatgtataag atagaataga acagctaagt
      121 aatgtataca gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa
      181 gtaatcaaga aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa
      241 atgatatctg ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca
      301 gaaagaatac ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga
      361 aggctctccc ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga
      421 aaagcgtgtt ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt
      481 gaagtgacag aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc
      541 ccaccatctg ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct
      601 gcgcctaaaa tgattccgca caattctgta aataaattta attttttcat ttctagcctc
      661 ttttcttttg aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag
      721 aggaaaatga aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga
      781 aaaatgcgag cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat
      841 caaggagttt ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat
      901 aaagcaaatc aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac
      961 atctttcttt ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa
     1021 ctcattcctc tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata
     1081 agaaaacaag acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg
     1141 tcatgataca gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt
     1201 tctactttat tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata
     1261 gctgattcca agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga
     1321 accaaaaagt tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt
     1381 atttttttct tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa
     1441 aggaaagaaa tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat
     1501 acaaacatta agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat
     1561 ttctctcatt tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc
     1621 caatcataaa aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc
     1681 ttcacttgcc ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt
     1741 gattgcactt gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc
     1801 tgaaattgac ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg
     1861 cttgctgaaa gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc
     1921 cttagtttta atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg
     1981 gacatttatt ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat
     2041 cagttctgcc ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa
     2101 acctcgatat tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt
     2161 atatcagtct gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga
     2221 acctgaaatc ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt
     2281 tgaattaaaa ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca
     2341 aatcgatatc tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga
     2401 agcaatgaaa agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt
     2461 gaataagcaa ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt
     2521 ggcagtcaag agatcttttg cgaacgaaca tcaacttaaa aaaatttccg atttggcagc
     2581 tatctctgat cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata
     2641 taaaggaatc caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag
     2701 tttgcgttac caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga
     2761 tagccagctc aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc
     2821 ttatcaaggc gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa
     2881 agcactgaat aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca
     2941 agtcaatgtc gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg
     3001 attagtaaag gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag
     3061 tatgatggaa agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt
     3121 gttttggttg gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta
     3181 tctaaaccat cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg
     3241 cagaaaatgc ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg
     3301 acagtcaagc aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga
     3361 gcagaccgtg tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat
     3421 cgtatgccta gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt
     3481 gctgcaagcc cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca
     3541 agaaactcat tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt
     3601 ttcgttacgc acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag
     3661 gatggagaaa ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac
     3721 tacgttcggc atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta
     3781 gtcgtcaatg gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt
     3841 gatacgccta tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta
     3901 gaaaagcaaa gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga
     3961 gaggaggaag gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt
     4021 aagtgctgct tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct
     4081 atggggcggg acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt
     4141 agaggcacgt aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc
     4201 caactgggaa gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg
     4261 aaaaaaacaa ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga
     4321 tgattcatct attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac
     4381 cgggcaaaga cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga
     4441 ctttttatca ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc
     4501 ttttgagcta gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa
     4561 tcaacggcca ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga
     4621 agcatctggc attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac
     4681 tcgctatcgt ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac
     4741 tgggagacag ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa
     4801 acacggaatt gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg
     4861 agatattgtt tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta
     4921 tgtagctaag cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac
     4981 gatcgtctac gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc
     5041 gtcagatcaa gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga
     5101 accagttgct aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt
     5161 gattagcgag caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt
     5221 gactaaaaaa gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc
     5281 ctatttattg aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc
     5341 tacactttaa aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt
     5401 gtgaacttag acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac
     5461 gcttgaagcg ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa
     5521 gaaaggaggt aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt
     5581 aattatttgg cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc
     5641 tgctgctgta ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac
     5701 gacattaaat gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc
     5761 cttggcaatg gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc
     5821 acaaatgttg ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat
     5881 gcttccaaaa gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc
     5941 ggatacattg aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga
     6001 tacagggaag atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg
     6061 tttaaaagtg gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc
     6121 tcaagcaggc ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt
     6181 ttttgctgcg atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt
     6241 attgaaaaaa gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa
     6301 aaattatggc ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa
     6361 acaagtagca aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta
     6421 ccgaatacta atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct
     6481 ggtttaaatg tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt
     6541 gcggacaaag cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat
     6601 ggacagaata taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc
     6661 gccaccctat cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca
     6721 ggttggcaaa gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg
     6781 gacttttcaa aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa
     6841 tcctcagata tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt
     6901 attgaactaa actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg
     6961 gtaattatta cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg
     7021 gatgccacga aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg
     7081 ttgcaaaaat atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg
     7141 tcaaaaaatt atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc
     7201 aattatacag cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa
     7261 ctattcacta atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact
     7321 tgaatgtagt cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag
     7381 atacctttac atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt
     7441 taacaggagt ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg
     7501 gtctccatac agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg
     7561 tctatcaaaa cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag
     7621 taagggatta tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat
     7681 tatctggcgg gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt
     7741 acttgcttct tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag
     7801 actggatgtt gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata
     7861 tcgaagaagc aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg
     7921 tgattgctga gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat
     7981 cagagttgaa gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct
     8041 cattgtcatt tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg
     8101 acgatcatca attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt
     8161 aagactcaag ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa
     8221 caagttgagc cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt
     8281 tggacgaatg taccgatgac tacacaaaaa aagtatttga gcgacagctt gcttttgcag
     8341 ccataaatga gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt
     8401 atatccaagc ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac
     8461 aagactatat tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag
     8521 cagtctggca actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac
     8581 tacatgcagc agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc
     8641 ttgaagaaca gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa
     8701 aaaatactta tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga
     8761 cacaactagt gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa
     8821 taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac
     8881 cataacgtga agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac
     8941 catttaagta agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt
     9001 aagaaccgct tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag
     9061 tagcagcagt ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt
     9121 tttccgtttg aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa
     9181 cggctgcttg aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt
     9241 gtcctgagta
//
LOCUS       DAYYNG010000116         9176 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000116 DAYYNG010000000
VERSION     DAYYNG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.117"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACHBFA_002153"
     CDS             <1..243
                     /locus_tag="ACHBFA_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFM9395592.1"
                     /translation="CTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQK
                     GSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            259..408
                     /locus_tag="ACHBFA_002154"
     CDS             259..408
                     /locus_tag="ACHBFA_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395593.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            482..1075
                     /locus_tag="ACHBFA_002155"
     CDS             482..1075
                     /locus_tag="ACHBFA_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9395594.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1091..1990
                     /locus_tag="ACHBFA_002156"
     CDS             1091..1990
                     /locus_tag="ACHBFA_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9395595.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1993..2478
                     /gene="ssb"
                     /locus_tag="ACHBFA_002157"
     CDS             1993..2478
                     /gene="ssb"
                     /locus_tag="ACHBFA_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9395596.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2844..3104
                     /locus_tag="ACHBFA_002158"
     CDS             2844..3104
                     /locus_tag="ACHBFA_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395597.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3544..3750
                     /locus_tag="ACHBFA_002159"
     CDS             3544..3750
                     /locus_tag="ACHBFA_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9395598.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3750..4001
                     /locus_tag="ACHBFA_002160"
     CDS             3750..4001
                     /locus_tag="ACHBFA_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395599.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            4016..4453
                     /locus_tag="ACHBFA_002161"
     CDS             4016..4453
                     /locus_tag="ACHBFA_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395600.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            4446..5153
                     /locus_tag="ACHBFA_002162"
     CDS             4446..5153
                     /locus_tag="ACHBFA_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395601.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            5318..5518
                     /locus_tag="ACHBFA_002163"
     CDS             5318..5518
                     /locus_tag="ACHBFA_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395602.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            5534..5713
                     /locus_tag="ACHBFA_002164"
     CDS             5534..5713
                     /locus_tag="ACHBFA_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395603.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            6054..6317
                     /locus_tag="ACHBFA_002165"
     CDS             6054..6317
                     /locus_tag="ACHBFA_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM9395604.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            6311..6661
                     /locus_tag="ACHBFA_002166"
     CDS             6311..6661
                     /locus_tag="ACHBFA_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9395605.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(6685..7299)
                     /locus_tag="ACHBFA_002167"
     CDS             complement(6685..7299)
                     /locus_tag="ACHBFA_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9395606.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            7613..8035
                     /locus_tag="ACHBFA_002168"
     CDS             7613..8035
                     /locus_tag="ACHBFA_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395607.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            8045..8248
                     /locus_tag="ACHBFA_002169"
     CDS             8045..8248
                     /locus_tag="ACHBFA_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395608.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            8459..9043
                     /locus_tag="ACHBFA_002170"
     CDS             8459..9043
                     /locus_tag="ACHBFA_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9395609.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         3290 a         1454 c         1758 g         2674 t
ORIGIN      
        1 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
       61 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
      121 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
      181 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
      241 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
      301 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
      361 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
      421 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
      481 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
      541 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
      601 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
      661 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
      721 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
      781 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
      841 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
      901 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
      961 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     1021 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     1081 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     1141 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     1201 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     1261 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     1321 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     1381 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     1441 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     1501 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     1561 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     1621 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     1681 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     1741 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     1801 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     1861 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     1921 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     1981 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     2041 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     2101 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     2161 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     2221 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     2281 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     2341 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     2401 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     2461 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     2521 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     2581 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     2641 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     2701 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     2761 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     2821 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     2881 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     2941 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     3001 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     3061 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
     3121 agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     3181 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     3241 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     3301 agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     3361 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     3421 taaatggcac gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa
     3481 atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca
     3541 aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat
     3601 catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat caaaagctat
     3661 cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattacatt
     3721 ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa
     3781 aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagctt
     3841 tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc
     3901 tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa aacatggaaa
     3961 cttatgaaat cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc
     4021 tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga
     4081 ggatatgacg ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt
     4141 gtcagtcgca gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta
     4201 ctatcgaaca ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact
     4261 atgcttggaa gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga
     4321 actgacttat gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcgactggct
     4381 agatactttt gaaagtatcg aaaaaaatgg tgcattttat acgattgaat cggaggagaa
     4441 agataatgaa taaggtggtt tatcagctaa caacagaaga tgtttcgcaa tttttaacaa
     4501 gcaaagaaca cttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag
     4561 aaatgaagta ctttattcaa agagtggaag aacagttgta tatggaaagc gaaacaagtc
     4621 aaacgaaggt cacagtcagc cataatcatt tagacgtgta taaacgtcgt cctgaaacgg
     4681 taaaaaaagg aacagagctt ctacgactag aaacagaaaa atacatcatg aatgtgtacc
     4741 tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgtagg aaacactatt
     4801 actcagaatc agaatattat tcaattttcg agagcatgga attaatggaa gaagaaatgg
     4861 aagagctaaa cagcaaaaat gcgacggtag acgattatta ccttgtattt actaggtttt
     4921 tgacgtgtgg gacggaatct atcgtgttaa tgggaatgga ttatgaactc gctgtacccg
     4981 aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga
     5041 ctttaactga ttttttagac acctacactt acgacgatac agaagaaatc caaaatgaac
     5101 ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa taaccattta
     5161 taccgaaaag tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt
     5221 cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataagcggg
     5281 caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca ttcctctatt
     5341 gttcgtactg tttgttggcg agatacttat gcgtcatgct gcagctgtac atttaaactt
     5401 cgtagtattc ttaggaatac tggtattttg tttgatcttt tggcgatttg tattagccct
     5461 tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa
     5521 ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga
     5581 aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg aatctaagat
     5641 tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atataccagc
     5701 actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac ttaattgttc
     5761 ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat aactatgaaa
     5821 tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc tgattctaaa
     5881 gattttgttt tcttaaacat attatgattt cctttcgact attttcatat aaatgttaac
     5941 atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa ttaagtatat
     6001 acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct actatggaaa
     6061 tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag tatattatgg
     6121 aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa gtaaacggtc
     6181 agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt gaacagttat
     6241 ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt gaagcagtag
     6301 gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga atacagcacc
     6361 tcgatctgga cacgaacaga caggtaaaag accttatatt gttttaagtc atgatattat
     6421 tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta gaaactatcc
     6481 gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag tattattaga
     6541 tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa aagcgcatga
     6601 tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag taagcaaata
     6661 aaatagaaac agcagcatga tcaactattc gtgattgacc atgctgtctt tttttattct
     6721 atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg caacaccttt
     6781 ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt ttgcattggc
     6841 actgtatagc ttaggacgtc ctttataaac accgttcgct tttgcaatct taatgccttg
     6901 tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca aaatttgaat
     6961 gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg ccatgatcgg
     7021 tagacttgtg atgatcagct gcacattttt cttcttcaag taattgactg aagcgatgat
     7081 ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat cttgatctcg
     7141 gacgaaatcc agtgcttctt gaaaaacaag tcgatgagta atcgttgcgc cagattgttt
     7201 ctcaatgaaa attctctctg caccgaattt tttcatttct tcaatctgcc gttgttcatt
     7261 ttgatcgatg gatgagacac gtatgtatgc aactctcaaa atttttacct actttctgca
     7321 actatttaaa aaacacccta atcatacacg agaaaaccag atttttcaat gtcgcggtcg
     7381 tgtctagtta gggtatacct tatatagaca aaaactaatg atggaggaaa tagtatgtgt
     7441 cacttttctt tttaaatggc aatatttttt gtaaataaat gtcaatcaag aaaatttaaa
     7501 cgattatgac attaaaaata tattttatga attttctagt atataacttg atttatctgt
     7561 taagatttat taagaaaacg ttttcttatt tttgatttcg aggtgataga caatgaaaaa
     7621 taagtttatt ttatctttag gtattttaac tattggaatt agtgtaactg gaactacaat
     7681 tcctacattt gctgatagta caaatgaacc aactactgta gtagataata accaagaatt
     7741 aactccttta gataggaacg gaatagccat tggtaaccca actaacgact taactgttac
     7801 tgaaaattat ttaatagttt ctccattact tgctcgagca gcttctaaaa cgatacgagt
     7861 gacaggaacg atgatttttg ataaagttgg ttggccttct agacctcctc aatcacggaa
     7921 ttggaaagaa aaaagaaatg gttattggta ctatggaaaa ttgtatttac aaagttataa
     7981 atcagctgga aacaattgga ttgcgactta ttcaggaacc ttaactagaa agtagggatt
     8041 ttttatgcca gaatttgtga acacaccaaa tttttatcaa gatagcgata ttctaagtaa
     8101 aattaattta ccgaaagaag aatcagcttt acaagttcag cgaactttac aggagacaca
     8161 aaaaattcaa aaatcaattt ctaatctaga agattctgaa gaaaaaagaa aaaaaagaga
     8221 ccaaaaaaca tatcaaaaag aaaaataatg attttaaaaa aagagtgtct agtcgacatt
     8281 ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt tctttttttg
     8341 ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa tatctatgtt
     8401 cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag tgattttttt
     8461 gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac aagaagacag
     8521 cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg caaaaagtaa
     8581 tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac ttgtcgtttg
     8641 gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca atcgactgaa
     8701 tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca aatcaagttc
     8761 tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc gtaatttgat
     8821 cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt ttggtggtcg
     8881 tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg atagcggtac
     8941 acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt atcgttattt
     9001 ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt catttaatgc
     9061 tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt ttgtaagtat
     9121 ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa tattaa
//
LOCUS       DAYYNG010000117         9024 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000117 DAYYNG010000000
VERSION     DAYYNG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.118"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..584
                     /locus_tag="ACHBFA_002171"
     CDS             <1..584
                     /locus_tag="ACHBFA_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9395610.1"
                     /translation="YKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTN
                     IVSPAVRGVFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRV
                     ADQSPITINLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLD
                     VYENAELKIEFSKQNHYFFVEMYLASRKSKSAY"
     gene            906..1133
                     /locus_tag="ACHBFA_002172"
     CDS             906..1133
                     /locus_tag="ACHBFA_002172"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9395611.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1256..1582
                     /locus_tag="ACHBFA_002173"
     CDS             1256..1582
                     /locus_tag="ACHBFA_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9395612.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1650..2363
                     /locus_tag="ACHBFA_002174"
     CDS             1650..2363
                     /locus_tag="ACHBFA_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9395613.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2666..3517
                     /gene="licT"
                     /locus_tag="ACHBFA_002175"
     CDS             2666..3517
                     /gene="licT"
                     /locus_tag="ACHBFA_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9395614.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3638..5503
                     /locus_tag="ACHBFA_002176"
     CDS             3638..5503
                     /locus_tag="ACHBFA_002176"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9395615.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5517..6960
                     /locus_tag="ACHBFA_002177"
                     /pseudo
     CDS             5517..6960
                     /locus_tag="ACHBFA_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            7068..7769
                     /locus_tag="ACHBFA_002178"
     CDS             7068..7769
                     /locus_tag="ACHBFA_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395616.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7895..8407
                     /locus_tag="ACHBFA_002179"
                     /pseudo
     CDS             7895..8407
                     /locus_tag="ACHBFA_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(8805..9014)
                     /locus_tag="ACHBFA_002180"
     CDS             complement(8805..9014)
                     /locus_tag="ACHBFA_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM9395617.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         3050 a         1484 c         1980 g         2510 t
ORIGIN      
        1 attataaaag tctactgttt tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt
       61 tggaattttt agaaatatta gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac
      121 aacagctaac aaatattgta tccccagctg tcaggggtgt ctttttaaaa tttctagaaa
      181 aagcagaatc ggagaaaatc ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc
      241 cgattgatct ggttgttttt attcgctgtt tatctatttt tatttctaac gcattcgatc
      301 atcgtgtagc cgatcagagt ccgattacga tcaatctgct ggaagaaaaa gagggatcac
      361 gttttagtat agcgaacaag gcaaatccaa cgaatttgac gctatcggag atggtcaaaa
      421 gaggaaaaac ctctaaaaaa aatggcgggt taggtctata cagtgcgaaa aaaatgttgg
      481 atgtttacga aaatgcagaa ctaaaaatcg aattttcaaa acagaatcac tatttttttg
      541 tagaaatgta tctggcatct agaaagagca aatcagccta ctgacttact gtcaaaggat
      601 aagtttgagg aaagtctaca cggagataga gacaacaaca cagcatattc aaaagaaaca
      661 gaagcgtgat gaccgttgtc acccttctgt ttcttttgct atgtagttaa acaaagtagt
      721 tgacacttcc gctacttagt attagtacga aagtatactt catgatacta agtagataag
      781 aagtgaaaag cggaacccac gatcgtcaaa atgctaactg ctttgcttac accagataaa
      841 gggaagagca atatcgctta ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa
      901 aaaaaatgct gacccagcag ttaagagagt tagaacaaga tgaaagtatc catcgaaaag
      961 cctataacca agtcccgcct aaagtagagt actcgctcac cgaagaagga aaatcattaa
     1021 gagagattct agtagcgttg tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct
     1081 caagggaaga agacacgcta ctgcatacac atggtggcta cctaaattat tgattttcta
     1141 tgacatttat tttaaataaa agaaaacagg agttttttta aaagcatcat ctatttttta
     1201 tgtaaaaaaa tggtatgatt aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa
     1261 tagtatggaa aaagtcaacc agtttagaga tgaacgtaat tggcgacaat ttcataacga
     1321 aaaagatttg gctatctcta tttctttgga agcatctgaa ttgttagaac tttttcaatg
     1381 gaaaacgccg gaagaagtaa aagaaacaca attagaacga atcaaagaag aactggcaga
     1441 tgtattgatc tattcataca tgatggcaga caaccttcac ctggatctag atgagatcat
     1501 tgaagaaaaa ttgatcaaaa ataatttgaa atatcctgta gaaaaaagcg caggtgtacg
     1561 aaaaaaatac acagagttgt agaaatgtgt cattcggctg ttggaaagag caaaagaaaa
     1621 acagtaaaat gagtctggag ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc
     1681 tagtcaatat cctcttgttc ctcttgttat tagtaggact agctttgatt ttcaatgaac
     1741 agatcaagga ttattttgtc agagagacag gagataaata tgctatagcg aatgtaacaa
     1801 aagaagatct gaaaaagaat aacgataaag atgtcagttt tgactttgat gcggtagaac
     1861 caatgacgac agaaggggtc atgaggtcgc aaatgagagg aacagatcta ccagtgattg
     1921 ctagtatcgc agtcccttca gtttcaatca atttaccggt tttcaaaggc ttggataata
     1981 cgtcactact ttacggagca ggaacattat ctccagatca agaaatggga aaaggaaatt
     2041 atgccttagc aagccatcgt gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt
     2101 tagagatggg tgccaagatc tatttgacag atttagaaaa tgtgtataca tacaaaacat
     2161 tttttaaaga aaaagttgct cctacagata cccaattact gaatgaagtc gagggaaaag
     2221 aaattgtcac actaattact tgtggcgaca tggacgccgt cacgcgacta gttgtacaag
     2281 gagaactgga aagtgttaca tcgataaaag atgcgacaga tgatatgaga tctgctttca
     2341 atctagagac aaaaacattt taggatactg ggacataagt cgttacagat taaaaacagc
     2401 caaaggatat gaatcgataa ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag
     2461 gtgcatctct gatttggttg ttttctagat agacttgtgt tccagttttt ttatatgaaa
     2521 aggcttgcat aaataaaaat tcgatggtat gatgattttg aataagaaat ggattgttac
     2581 tgtacggcag gcaaaaccag aattcagcta agaaacagta ggcgaattct aggttttttt
     2641 atttttcttg agagggcgaa gcaaaatgaa aattgataaa attttgaata acaatgtcgt
     2701 catttcgaaa aatggattcg gggaagaagt cgtttgtatg ggaaaaggac tagcttttca
     2761 aaaaaagatt ggggacgaga tttcgccaga agcggtccaa aaagaatttg ttttaagaga
     2821 ttcttttgct aaaaaccaat tccagcagct gatggctgat attccggcag aggagatcga
     2881 atgggtcaaa caagtagtcg aactggctga agataaacta aaagttgaat tttcgcctaa
     2941 tatttatctg acattgaccg atcatcttca ttatgcgatc acacgagcaa aagaaaatat
     3001 ccaactgcct aatccattat tatttgaaac aaaaaaattt tatccgaaag aatatcaagc
     3061 agcgaaagag gctcttttgt tcgtgaaaca aaagacgggt gtggaacttt ctgaggatga
     3121 agccggtttt attgcctttc attttgtgaa tagccaacaa ggaaacgaag acatgcaagt
     3181 gacgatgacc gctaccacaa tggtaagaga tatactgagt atcatcagta aattcttcgg
     3241 catcgtgttc gatgaagaat ctttgaatta tcagcgtatc attactcatc tgcagttttt
     3301 tgcccagcgt tatctcaaag gggaaggatc tgacgagcaa gatgaatttc tctacgcatt
     3361 agttcaagga aagtatccaa aagcgtttca ttgtgtcgaa cggatcaatg agtatcttcg
     3421 acaaacgcaa aaacaggaaa tggggatcgc agagcagatt tatttaacga ttcatattca
     3481 aagagtagta agtgagaaaa aaagcatcca atcataagga ttgttactgt atggcaggca
     3541 aaacctggat tgaaaaaata ggttcacgaa tgtttgtgac tgtttttttc aatctgggtt
     3601 tttatttttt aaataaaaaa gaaagaagga aaaaacaatg gataatcaag cagttggtcg
     3661 tcgtgtatgg gaagcggtag gaggacaaaa gaatgtcaaa agtttagtac attgtgctac
     3721 gcggttgcga ttcagattaa aagatgaatc tttagcagat acgcaaaagt taaaagaaga
     3781 cccggacgtg atccaagtcg tacaaagtgg cggtcagtat caagtagtga ttggcagtaa
     3841 tgtagcagat gtttatcaat cgatcgtgga tgaggaaggt cttgcagttt cggaggatgg
     3901 aaaagaagag tccaaaaata ttttgaatcg tctgatcgat attatctcta gtatatttac
     3961 accttttcta ggggcgatgg cagccgccgg ggtattgaaa ggattcttat cattggcaac
     4021 ggtgatgggc tggctgacag ctgatagcgg tacttatcaa atattgtttg ctgcagcaga
     4081 tggtgtcttt accttcttac ctgtcatgct tgcttttaca gcagctaaaa agttcaaagc
     4141 caaccagttt ttagcagtag caattgctat ggcacttgtg tatccagcta tcacagcagt
     4201 tgctggtgcg ggagaagcta tcagtttctt tgggatccca gtgattcttt caccatctgg
     4261 ttatacttct tctgtcatac caatcattct agccgtatgg gttcaaagta aattagagcc
     4321 attcgtaaaa aaagtgattc cgcaatttct gcaaatgatt ttagttccgc ttgtcgtttt
     4381 agtagtgatg gttccattaa catttcttgc tttaggacca atcgggacgg ttgccggtaa
     4441 tgctttaggc ggtctcttca actcgattta tggatttagt ccaatcgtag caggtctaat
     4501 catgggaagt ttatggcaag tattcgtgat gttcggcatg cattggggat tcgtaccgat
     4561 catgtttttg aatatcgaac aatatgggtt tgatgtattg atgccaatgc ttcttccagc
     4621 catcttggca caaggtgggg ctgctttagc tgtagcattg cgaacaaaag atacgaaact
     4681 tcgagcatta gggatttctt ctacagtgac ttcgttattt ggaatcactg aaccaactgt
     4741 gtatggtgtg acattacctt tgaagaaacc attcattgcc gcttgtattt ctggaggtat
     4801 tggtggagct attatcggat tttccggagt aaaagctttc tccagcagtt tggtcagctt
     4861 attgacgatt ccgactttca ttaatacggt ggatggtgta gagtcaaatg tgacagtagc
     4921 agtaatagct acaggaattg cttttgttct tgcattcgta ggtacattga ttttaggatt
     4981 tgacgaacaa acacaagaca atcaattaga aaacaaacat gcgaatgcag gagaaccgat
     5041 tacttcagca cggcatacct taaaaagtcc tttaacgggt aaagtactgc cattgagtga
     5101 agtaccagac caagtttttt cttctggcgt gatgggaaaa ggtatcgcta ttgatccaga
     5161 agtaggagaa ctagttgcac ctgcagacgg agagatcacg actatttttc caactggaca
     5221 tgctgtaggg atcacaacga ctgacggagc ggagattttg attcatatcg ggatggatac
     5281 agtagaatta aatgggaatg gctttgagat cttagtcaaa caaggggatc tcgtcaaagc
     5341 aggagattta ttgattcgtt ttgacatcga agcaattaga gcagctggct atagcgtaat
     5401 cactccagtt gtcattacga atactgatgc atttgccgat atacttgaat tagaccaaaa
     5461 agagattatt gcaaacgaag atgttctggc aattgtaaaa taaggaggaa atcaggatgc
     5521 aatcacgttt ttcagaaaaa tttttatggg gtggagcaac tgctgccaaa tcaattagaa
     5581 ggggcttatg atcgagacgg aaaaggacta tccgttgcag atgctatgcc tggaggtaag
     5641 cagcgctttg cgattatcgg cagtgaagaa tttgactgga cgattgatca agaaaaatat
     5701 atttacccga atcatcgagg aatcgatcat tacgatcgtt tcaaagaaga tcttgccttg
     5761 tttgcggaaa tgggattcaa atgttatcgt ttttcgattg cctggacacg aatttttcca
     5821 aatggagacg aaggtactcc taacgaagcg ggattagaat tttatgatca gctgattgat
     5881 gagtgcttaa aatatgatat tgaacctgtg atcacgattt ctcattacga aatgcctctt
     5941 catttggcaa aagaatacgg tggctggaaa aatcgaaaac tgatcgactt ctatgaacga
     6001 tttgctcaga cagtattaga gagatacagc agtaaagtca aatattggat gactttcaat
     6061 gaaatcaatt cggcctttca ctttccagct ttaagtcaag gattagtaaa aagcaatgga
     6121 gcaggagagt accaaaatat tttccaagca tggcataatc aattcgttgc aagtagcaaa
     6181 gccgtaaaaa tcggccacga attgcgttcg gatatccaaa tcggctgcat gattatctat
     6241 gcaacaactt acagcatcga tgccaatcca gtcaatcagg cagcaacaat gatccaaaac
     6301 caagaattca attttttctg cacggatgtc caagtacgtg gagaataccc tgcatacaca
     6361 gcgcgtaccc ataaaaaata tggagtcgat ccggaaaaat tagagcaaac ggaaaaagac
     6421 ttccagttgc taaaagaata tccagtggac tacatcggat ttagttatta tatgtctacg
     6481 gcaatcaatg agactgatcc acaagcagca acttcagaag ggaacttgct gggaggagta
     6541 aaaaatccgt ttcttgaggc aagtgaatgg ggctggcaga tcgatccaga agggctgaga
     6601 atcgcattga atgaattata caatcgttac cagaaaccat tattcattgt tgaaaacggt
     6661 cttggagcca ttgatcatat ggaagaggat ggcacgatta ttgatgatta tcggatcgat
     6721 tatttacgtc gtcatatcga agcgatggct aatgcagtag cagacggtgt agatttgatg
     6781 ggctatacgc cttggggatg tatcgatctt gtcagtgcat caacaggaga aatgagtaaa
     6841 cgttacggct ttatctatgt ggatctagac gatgaaggaa atgggacact gaatcgatca
     6901 aagaaaaaat cttttgattg gtataaagaa gtaattgcct caaatggcgt aaacttgtaa
     6961 ttaaagcagc tgaatgaaaa agaacacact tctcacatgt gatttgtgtt ctttttttat
     7021 tttttatgtg ctattctttt agtaatagta aagcaaaggg tgacgttatg aaaaggacac
     7081 gtgtactata cttggaagtc gcagataaaa ttaaagaaga tattttctca ggaaaatatc
     7141 cagtcggttc gatgttgccg acagaaagtg aattagaaga actgtttcaa gtcagcaaaa
     7201 tcactgtccg aaaggcaatc gaactgctgg catcagatga atatgtcgca aagaaaagtg
     7261 gtcgtgggac gacggttcta agcaatcgtc cttacaatcg attatccaaa gcggcaagct
     7321 ttacgcaaat cttggagaac tcccgatatg aagtccggaa agaaacgctg gatttgcaat
     7381 tggtccatat aaagaaggaa gatcctttgt tttccttttt cggtgagcaa gctgtttgtt
     7441 ttaagcgctt atattatttg aacaatcagc cttatattta ttttgttcat tatcttccac
     7501 aagaaatgat gcaattcagc agagaagaat ttggcaaaga atctttgtat cgtttgctga
     7561 cgaaaaaagg atatactatt gactcatttt ctgatgattt ctcggcagta tttttaacag
     7621 aagaagaaca aggaattctg aaaacagacg cacagatcgg catcaaacgg atacggaaat
     7681 cgtctacgca aattggggaa atgattgaat attcggaggc ggtctaccat acggagctgc
     7741 atccttacca tattgattat gaaacataaa taaaaaaaga ggttgtgaaa aagctgccct
     7801 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttagg
     7861 cttattaccg cagatacacg gctgtttctg gattaccatt tattctggat aagggggtgt
     7921 ggcacgattt tcagaaggaa tctatgaacc aatcactgta ttgattttag cttcgatggt
     7981 aaccccattc ggcttgacga tcgcttattt cttagggaaa atcattcgta aaaatatact
     8041 caatcgtcaa gaaatcgata cattgaagac cgcttttcct atggggatct gtcagatcac
     8101 agagggatgt ttccctatcg tattgaacga cttggcacgc aatgtcatag caacgggagt
     8161 cggtgggaca gtaggtggcg gattaagcat gttttggggc gcagatagcc gtattccggc
     8221 atcgggaatg tttgcagtcg ctacgatgac cagaccatgg gcattcattg gtgcattgtt
     8281 agctggttct tttgtcacag gaataaccat tttattattg aaaaaaccag tagatccgaa
     8341 tgcagaaatc atccaagaag aaaaagagga agaagacata tcttgggatg atttgacaat
     8401 tagttaggag aggcagaatg gattacaaga aaaaagtaca atagatgtac cttgagacag
     8461 gaatggtatg aaagaatcag agttagaaag aatcgaatat acagattttg gtttaggagc
     8521 gatcgaaact gtccagagca tcggcaccct ccttgaaatg acgatgaagg aaaaatgaga
     8581 acctttcgtt gtcgaaaggg tctggtatac cttatgtcga aggaaaggaa acaaaaaaac
     8641 tttcctgtac tattccaaaa aggaaagaaa gttattatac agtaaaaaaa gaaattattc
     8701 ttcatgctag agatcagtac acgttcgagc ccaatatcaa acactagttc caggcaggcg
     8761 caacaggtgc ggttatcact gaattttctt ccagcagtga cgattcaagt gatattttta
     8821 caaatccagc tatttatcgt taacaaaaag atttttagga gaagctgatg aacaaaaatg
     8881 atcaaatttt gatcgtttag ttcgtcggct tttttattta gagaaacatc atttatcagt
     8941 gaaacataac acgagaacaa aatgattttt tcgttaataa aagaaacaag tatttgttgt
     9001 ttaaaataat acacgttatt ggca
//
LOCUS       DAYYNG010000118         8942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000118 DAYYNG010000000
VERSION     DAYYNG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.119"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACHBFA_002181"
     CDS             <1..260
                     /locus_tag="ACHBFA_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395618.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACHBFA_002182"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACHBFA_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACHBFA_002183"
     CDS             complement(1379..1984)
                     /locus_tag="ACHBFA_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9395619.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACHBFA_002184"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACHBFA_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACHBFA_002185"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACHBFA_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACHBFA_002186"
     CDS             3019..3990
                     /locus_tag="ACHBFA_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9395620.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACHBFA_002187"
     CDS             4130..5443
                     /locus_tag="ACHBFA_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFM9395621.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACHBFA_002188"
     CDS             5486..6352
                     /locus_tag="ACHBFA_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9395622.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACHBFA_002189"
     CDS             6349..7197
                     /locus_tag="ACHBFA_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9395623.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACHBFA_002190"
     CDS             7211..8683
                     /locus_tag="ACHBFA_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9395624.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWVKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACHBFA_002191"
     CDS             8700..>8942
                     /locus_tag="ACHBFA_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9395625.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1514 c         1830 g         2768 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccatgtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggtg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAYYNG010000119         8836 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000119 DAYYNG010000000
VERSION     DAYYNG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.120"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(434..2815)
                     /locus_tag="ACHBFA_002192"
     CDS             complement(434..2815)
                     /locus_tag="ACHBFA_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9395626.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2805..3452)
                     /gene="recU"
                     /locus_tag="ACHBFA_002193"
     CDS             complement(2805..3452)
                     /gene="recU"
                     /locus_tag="ACHBFA_002193"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9395627.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3516..4055
                     /locus_tag="ACHBFA_002194"
     CDS             3516..4055
                     /locus_tag="ACHBFA_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9395628.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4145..4564
                     /gene="gpsB"
                     /locus_tag="ACHBFA_002195"
     CDS             4145..4564
                     /gene="gpsB"
                     /locus_tag="ACHBFA_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9395629.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4586..4953
                     /gene="rnpB"
                     /locus_tag="ACHBFA_002196"
     ncRNA           4586..4953
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHBFA_002196"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5162..6328
                     /locus_tag="ACHBFA_002197"
     CDS             5162..6328
                     /locus_tag="ACHBFA_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9395630.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6374..7870
                     /locus_tag="ACHBFA_002198"
     CDS             6374..7870
                     /locus_tag="ACHBFA_002198"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM9395631.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7951..8634
                     /locus_tag="ACHBFA_002199"
     CDS             7951..8634
                     /locus_tag="ACHBFA_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9395632.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8643..>8836
                     /locus_tag="ACHBFA_002200"
     CDS             8643..>8836
                     /locus_tag="ACHBFA_002200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395633.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2790 a         1591 c         1863 g         2592 t
ORIGIN      
        1 ttttcagctt ttttattctt ggaataacga ggtattgttt tcgataaaaa aagatggtgc
       61 agacaaatta tgataattaa gcatactatc agataactca aaatgaagcc ttctttcaaa
      121 aaattattca aaaccatata ttgtattttt tatttgataa taacaatggg attattaaga
      181 agtggcacta aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg
      241 gataaagtat atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta
      301 gatcacacat gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat
      361 gatttttatt tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt
      421 ggtttctttt ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat
      481 ttgcagcatc cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag
      541 acggtgtcga gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc
      601 ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag
      661 agctagagct ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg
      721 aagatggaat agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt
      781 agtacagttc accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca
      841 cgttagctga aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt
      901 ctgaagtgac aggcgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg
      961 gcgtatagcc cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat
     1021 actcgtcatc tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt
     1081 gcgcattcgt accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag
     1141 ctgtttctgg agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt
     1201 ggaagacgat tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg
     1261 cataggctgc tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg
     1321 tattactaga gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta
     1381 agaaagaagc cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac
     1441 gggataagta aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc
     1501 ctacaggagt cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag
     1561 aatatttcaa atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa
     1621 aatcacggga agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac
     1681 gtccgccaat ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt
     1741 gcatctcgtc gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct
     1801 tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat
     1861 ctgttttttc ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat
     1921 tgtcatcgga ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat
     1981 catattctgt ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc
     2041 ttttcttcgc ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc
     2101 cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct
     2161 cagaagctgt ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg
     2221 tcaagatctc ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg
     2281 ccttgcgttt cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt
     2341 gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac
     2401 cgatgatccg aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca
     2461 cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg
     2521 cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt
     2581 ctaatttttt atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac
     2641 ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc
     2701 cttttttatg tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct
     2761 ttttaggagt aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg
     2821 ctttctcctt caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc
     2881 cgcggagaaa taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt
     2941 gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa
     3001 taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg
     3061 aggcatgctt tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt
     3121 ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag
     3181 tctgtagttg aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga
     3241 tagtctactt tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg
     3301 gaaagataat atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg
     3361 aattcagtga tctgatttct tttttcttga attttttcga cgggagaggc attcttataa
     3421 taaggttggc catttggata gcgcaatacc atctttaatc acactcctag gccacattat
     3481 agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg
     3541 tgtctgggta tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag
     3601 ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg
     3661 tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga
     3721 aagaagaata tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata
     3781 actggaatga agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg
     3841 aagctgttag ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta
     3901 tacttgagca tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga
     3961 agtatttttt tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga
     4021 tcagtatgga cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac
     4081 ttttttccta tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt
     4141 aaatatggcg aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa
     4201 aatgcgcggc tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta
     4261 tgaaacttac agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa
     4321 agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc
     4381 accaaagagc gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg
     4441 tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa
     4501 tccaaataac tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt
     4561 ttaatttttg attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct
     4621 gaggaaagtc catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca
     4681 taagctaagg tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt
     4741 aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc
     4801 ggtaaacccc tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag
     4861 aacaggacaa aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct
     4921 ggaaaagcaa agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg
     4981 tgacaaaagt aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact
     5041 tcagatgaat cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga
     5101 gtaaaagtgt ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg
     5161 tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact
     5221 ggttggtaaa gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg
     5281 ttttgaagga acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg
     5341 agtaaaaatc gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt
     5401 aaaagcattg ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa
     5461 atcgatcaag tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat
     5521 cgttgaacgt ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg
     5581 cgcacatttt cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac
     5641 gacaggacca agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa
     5701 agaaaacatg gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta
     5761 tgatcctgta tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat
     5821 cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat
     5881 catggaaaat gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat
     5941 tactggttca gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat
     6001 cggattaggc tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa
     6061 agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac
     6121 tgttcggaaa ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa
     6181 atatatcttg actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa
     6241 aagaaaattg tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc
     6301 gcctagaaaa aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt
     6361 ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct
     6421 acaacaagct ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag
     6481 tgcataccga agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg
     6541 tccaaaaatc aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt
     6601 tggaactgcc gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga
     6661 agatcttatg agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc
     6721 tagagaaaca caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac
     6781 aaaacagttg ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa
     6841 tcaatacgaa cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga
     6901 aggaattctt tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt
     6961 tttaaatcgg agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca
     7021 acttggttat gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg
     7081 aattaatccg aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc
     7141 tgtttttgga gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa
     7201 atatgcgtat acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt
     7261 gtttaacgag atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt
     7321 tcaagaatgt gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt
     7381 gaaccataca aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca
     7441 tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga
     7501 actacctgaa atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga
     7561 tgtggaaggc atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc
     7621 ttatgcatta gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga
     7681 tgtggaaaat gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca
     7741 tatccatcaa tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga
     7801 accgcttaat ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca
     7861 ggtaaaataa aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc
     7921 agctatttac ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat
     7981 agtatggacc aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt
     8041 gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt
     8101 gcagcagctt acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca
     8161 aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg
     8221 attttttcca ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta
     8281 tctctggaag gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc
     8341 attttgcgta ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg
     8401 tatccgcaaa cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat
     8461 ttttatgaaa aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa
     8521 gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct
     8581 attgacgatg aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg
     8641 ttgtgacaga agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct
     8701 ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa
     8761 tttcaaaaaa tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag
     8821 gagaacaccg tttatt
//
LOCUS       DAYYNG010000120         8817 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000120 DAYYNG010000000
VERSION     DAYYNG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.121"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1354
                     /locus_tag="ACHBFA_002201"
     CDS             <1..1354
                     /locus_tag="ACHBFA_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9395634.1"
                     /translation="VNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIVYTHREKDCYTL
                     YFSEHIPRCKIMQQLTQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVL
                     AYFHDCGIEIDRYSPRFTEMERRLLLLNVAYRLGSFKSWELPESFFERADRFIESVTE
                     NSGRFYDKENKEILSIGFAISFLRQQLGTVTIDPTFIEEIKKRPIYNYVESTWETTDF
                     QTYYKKEEFVFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFETHF
                     RQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQANLYKDVQTLFSSWAKRL
                     PYDLRFNPNCMRAFVIELSGILRLTKERLTIYIVTNSDVHYLIYREALEAVTTFDFQV
                     APTIYASISDIKKYAQQPSNRVLCERTLYTPDAVQYENIIPISINTIERAIISAVQNK
                     "
     gene            complement(1515..1880)
                     /locus_tag="ACHBFA_002202"
     CDS             complement(1515..1880)
                     /locus_tag="ACHBFA_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9395635.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLIWGVCLSIKDFLKYLF
                     VHTEAKNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVPNK"
     gene            complement(1877..2242)
                     /locus_tag="ACHBFA_002203"
     CDS             complement(1877..2242)
                     /locus_tag="ACHBFA_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9395636.1"
                     /translation="MFHSNLIEFLEWIIAILNICSIGILVAGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEIG"
     gene            2683..2811
                     /locus_tag="ACHBFA_002204"
     CDS             2683..2811
                     /locus_tag="ACHBFA_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM9395637.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2868..4562
                     /locus_tag="ACHBFA_002205"
     CDS             2868..4562
                     /locus_tag="ACHBFA_002205"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM9395638.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKDTPMQDEITVTGLKMTNTLTQ
                     EEEHVTVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(4966..5826)
                     /locus_tag="ACHBFA_002206"
     CDS             complement(4966..5826)
                     /locus_tag="ACHBFA_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395639.1"
                     /translation="MKQIQNKWNKRIIVMISSVCLFCLTVMLCLISTLANQHFMLEQN
                     MQSDYAKKVAAEINREIQSYHYEIGAPAEALEKIVSEELVQKNIELFIRNRYEKEPVE
                     LIGEKEVEEKLQEIINTYKQEEAEDDPFASEENNLLIYMTSGIFRRGIQSIYLMSFID
                     QILTIRERMVSFLPMISSILLVLLSGVFYLYRMSWKKVLWSFAVVIGGAGFQLLSLSV
                     SLYLYDMASQLDIRTQALEHLLDTYILSFIQFFSVVAIVEVVIAAGLWWIGKRVNTEK
                     KVFSKRNGYK"
     gene            6117..7250
                     /locus_tag="ACHBFA_002207"
     CDS             6117..7250
                     /locus_tag="ACHBFA_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM9395640.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPRILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDETNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNNLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLEEAAQAYEIFDKKEDKNIKVVLKP"
     gene            7281..7595
                     /locus_tag="ACHBFA_002208"
     CDS             7281..7595
                     /locus_tag="ACHBFA_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM9395641.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAVFLTIRPILRIVRNIFIYDEKRTRKKHGK
                     AV"
     gene            complement(7732..8190)
                     /locus_tag="ACHBFA_002209"
     CDS             complement(7732..8190)
                     /locus_tag="ACHBFA_002209"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9395642.1"
                     /translation="MERRTVIKLRDRPTLIKETAEWFASKWHLPVAVYEESIQASTRK
                     NTSVPQWYVVLNEWGEIIAGAGVVDNDFHDRVDLTPNVCALFVEKDYRNQRIAKMILE
                     TIKQDFAEMGVKVLYLVTDHTDFYEKYNWQFIGMAQDNEGIPVRVYQMTL"
     gene            complement(8412..>8817)
                     /locus_tag="ACHBFA_002210"
     CDS             complement(8412..>8817)
                     /locus_tag="ACHBFA_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9395643.1"
                     /translation="ATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPI
                     VLNPIMFIPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPVIGGVLATKSIAGGV
                     LAAVNLILSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2796 a         1750 c         1567 g         2704 t
ORIGIN      
        1 agtcaatcta gaaaagctct ccaatcttct acatgtttgc aaaaccactt tactcaatga
       61 tatcaataac ataaaaaaag aatttgaaga acaaattgtt tatacacatc gggaaaagga
      121 ttgttacact ttatattttt cagaacatat acctcgatgt aagatcatgc agcaattaac
      181 ccaaaattcg ttgtttttaa aaacttgcct gctttatttg gaagaagatg aacctgatta
      241 tctccaatta actgagtgtg aattcatctc tgtcagcaaa gcttactcct taaaaaaaca
      301 agtattggct tattttcatg attgtgggat cgaaatcgac cgctactccc ctcgattcac
      361 tgaaatggaa agacgtctgt tgcttcttaa tgtggcttat cgtttaggca gctttaagtc
      421 ctgggagcta cctgaatcgt tttttgaacg tgcggatcgt ttcattgaat ctgtaacgga
      481 gaatagcggt agattttatg ataaagaaaa taaagaaatc ttatcgattg gttttgctat
      541 tagtttcttg cgccaacagt tgggtactgt gacaatagat ccaacattta tcgaagaaat
      601 caaaaaacgt cctatttaca attatgtcga gtcaacatgg gaaaccactg atttccaaac
      661 ctattataaa aaagaagaat ttgtctttat tttgacactg ttcaatcttt gtaattatgg
      721 tttccattct tatcagttga ttgcggaaga ttttcaacag ctacaccaag tgtttattga
      781 taatactccc gaaataaaag aactagttgc tacctttgaa actcatttcc gtcaagaatt
      841 attcgggaat cagccatttg aacgtgcact gattcatctg atgcgttcgg cttgggataa
      901 ctatcaatta ttcatgccgg aaaaatttta tcttctaaat gaggaacagg caaatttata
      961 caaagacgtc caaacacttt tttccagttg ggccaaacgg ctaccttatg atctacggtt
     1021 taatcctaat tgcatgcgag cttttgtcat cgaattgagc ggaattctcc gtctgacaaa
     1081 agaacgtctg actatctata tcgtcacaaa ttcggatgtt cattatctga tttatcggga
     1141 agccttggaa gctgtaacta ctttcgattt tcaagttgca ccaacaatct atgcaagtat
     1201 atctgatata aagaagtatg cacagcaacc ctcgaaccga gtgttatgtg aacgtacatt
     1261 atatacaccg gatgcagtcc aatatgagaa tattattcct atttcaatca atacgatcga
     1321 acgagcaatc atttcagctg tgcaaaataa ataagaggct gtgataaaca tcatgtcaca
     1381 gcctcttatt ctgaataaac aatattccaa aagtaacttc ttcggaaata agccgaaatt
     1441 ttcataaatt tcttcttatt ccttgaatct gcccacttct taacaacctc tcgataaacg
     1501 gcgggctcat agcctcactt atttgggacg tctactggcc cgatttcttt atttaaaaaa
     1561 taagaaatga tcgttcggat aactaccaat gcagctagct ggaaaatatc ctgcaaagta
     1621 ggtttgataa tcgattcgat gatcccagcc gaaacaagaa tttctaaact taggagtata
     1681 taacctccta acatcttttt aatagcattg ttctcgctga ctcggtcgtt tttcgcttct
     1741 gtatggacaa ataagtattt caagaaatcc ttaatcgaca gacaaacgcc ccaaatcaat
     1801 aaacagatag caaaaatatc aagtcccagt acaagttttt caaaatatgg aagaagaaac
     1861 tgcatcaact ctgtcgtcat cctatctctc ctcttctttg tttctctttg tcttctattt
     1921 cctgattcaa gaaataagaa atgactgttc gaatgataat aataagtgca agcttcaaaa
     1981 tatctgtcca agtcggcttg ataactgact cgatgatatc agcaacgact agcacttcca
     2041 agcttaacaa aatataactt gctaacagtt ttcttgtatc tgcattacga gtattacgtt
     2101 ctgcatagtt tttcttgaca tgagaccaat caaaaagttt gaatagaaca agaacgatcc
     2161 cagcaactaa tataccgata gaacaaatat ttaaaatagc aatgatccat tctagaaatt
     2221 caatcaaatt tgagtgaaac atataagcta cctactttct tttgatttgt tcctattctt
     2281 ttattgtaaa agaaagagac cttgttcgtc tatttaaaag ttgttttatt gatcacacct
     2341 tgcctaaaat cctcctcttc cctttctcat tcttcaccct ccataccatc catacatcat
     2401 ggaagtatcc aaaacaacat agcaagttaa aataaggctt agtccgtcat cagcttttca
     2461 aagtggcact gtctcgcgct ttttttaccc aaatttctaa aaatagaacc atacatctcc
     2521 tcaaaacttt tcttctttcc taccctatta ctaattacag gaaaatcaga cacttcttct
     2581 tttttgtcaa aaattccttt ttcttctcac cctatcattt ggcattctca taaaaacgaa
     2641 ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc
     2701 aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaaaaca
     2761 gggctattcg aagatgacag tcatttatat gatgaatttg aatccatcta gcatcttatt
     2821 tcgttgaatc actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta
     2881 aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag
     2941 aaggcaaatg gaaaggggac cagatcacct tttattcatt ggatgatcac ggttcaaatg
     3001 atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaatcat ccattagaaa
     3061 accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt
     3121 tgatggatct attaagtcga ataccttctg ttactgagcc aggaatgact gctgaagaag
     3181 atacgagaga ttttgatgca aaacaccgta cctttgacaa agcacgtttg atggaaggtg
     3241 gtaaagggat cattgacggc ggaaaactgg gcttcaacaa caaggaccgt attcttctga
     3301 ctaaactgat tgccatgcct gattcagaag aagaaaaact agacaatatc actatcgaag
     3361 aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa
     3421 catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga
     3481 tttatgaatt cactcaaatc gaacatttag tgggcgtcaa tcgaacacgt tacaatcagt
     3541 acgaaagtat catgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt
     3601 tgaaccgccg tgtcactgac tgggaattca aagatacacc gatgcaagat gaaatcactg
     3661 tcactggttt gaaaatgact aatacattga ctcaggaaga ggaacatgtc acggtagatg
     3721 aagatacagc ggtcattttc acgaatggtt ccattacaga ttcagctact ttgggagatt
     3781 acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag
     3841 ccagcgaacg tttctataac ttaggaaatc ccgacaaatt tttcgcggat cgagatgcga
     3901 gcgaatgggt cagctttaca ctgacgacga aaaaccacct gttattgaat gaaatcaccc
     3961 gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaact acaccgatca
     4021 cgccattagg ccaaaaagat gtcaatatgt ctatcgtcgt gcatcatcaa ccgcatttta
     4081 cgtctcaaaa accgaatgaa acagttattt ggggttattt cctttatcca agacgacgtg
     4141 gcgaatttgt ggataaacag tatattaaga tgaacggaaa agaaatgttg gaagaactga
     4201 tcggacaact ttcaaaagta gatcctggtc cagtgaatat tcgggaaaaa gaaacagaga
     4261 ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcattattta
     4321 ataaccgtgc aaaaagtgat cgaccaaaag tcattcctaa gcattcaacc aatctggcct
     4381 ttacaggaga atttgttgag cagccttatc agatgatctt tactgaacaa agtgctgtac
     4441 gttctggaga aatcgctgct ttccattttg cgggcatccc tatgtcccgt cttgtcaaaa
     4501 caccgcgtta cgacaaagac atccctactc tagctcgtgc cgcaaaaaaa atgtttgaat
     4561 aatcaagagg ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc aagcaaaaac
     4621 agctacttat gtttttgact tggttggact tattatcgca gatgcaagct tttgaacacc
     4681 gtttatcaag aggttgtgaa aaagacgttt aatttcgagt attaagaaaa attttgtgaa
     4741 aatggttcct cacatttttg cataatttgg cttaataccg agaaactggc ttttgaacac
     4801 cgtttaccaa gaggttgtga acctaccgtt tagctctgag tcacaagaag caatttcaaa
     4861 aaatagcttc ctatgtttta tgaatattgt gaccgaggag ttggtagggg aaccccattt
     4921 attcataaca agaggttgtg acagatttca tgtcacaacc tcttattatt tatatccgtt
     4981 tctttttgaa aaaacctttt tttcagtatt cactctcttg ccaatccacc ataatccagc
     5041 tgctatcact acttcaacga ttgctactac actaaaaaat tggatgaatg acaaaatata
     5101 tgtgtctaac aaatgttcga gtgcttgggt tcgaatatct aactgactgg ccatatcgta
     5161 caaatagaga ctgactgaca gacttaataa ttggaagccc gcacctccta tcaccactgc
     5221 aaatgaccaa agaacttttt tccaagacat tctgtataaa taaaacactc cgctcagcaa
     5281 tacaagcaag atactactga tcattggaag aaaagaaacc attcgctctc gtatcgtcaa
     5341 tatctgatcg atgaacgaca tcaaatagat tgattgtatt cctcgtcgaa aaatcccaga
     5401 agtcatatag atgagtaaat tgttttcttc actagcaaat ggatcatcct ctgcttcttc
     5461 ttgtttgtat gtattgatta tctcttggag cttttcttct acttcctttt ctccaatcag
     5521 ttctactggt tctttttcat atctgttgcg gatgaaaagc tcgatatttt tttgtactag
     5581 ttcttcagaa acaatctttt ccaatgcttc agccggagct ccgatctcat aatgatagct
     5641 ttggatctct cgattgatct cagcagctac ttttttcgca tagtccgatt gcatgttttg
     5701 ctctaacata aaatgttgat ttgccaaggt gcttattaaa caaagcataa cagtcaggca
     5761 aaacaggcag acagaactaa tcataacaat gatccgttta ttccatttat tttgaatttg
     5821 tttcattacg cctcctcgta tttgtttttt cactgaaacg aatgatggtt ggttattgtt
     5881 taaatagtta aaatgattca atagttcaac tatactttac tgtacgtaag gatacaatga
     5941 aggcacactc cttctttttc taataaaaaa aaattttgga actcttgctt ttttgaggat
     6001 ttttattttg agaaaaaagc gtttcattat caaaaagata agaactcctt tagaatgaaa
     6061 ctatctgtag taccaataat gcagacaatt ttgtgaatct gaaaggagca tctccaatga
     6121 aagcagtcac ttggcaagga aaacaaaaaa tggaaatcag aaacgtagat gatccaagaa
     6181 tattagaacc aacagacgca atcatccgta tcacagcaac cgccatttgc ggctccgatc
     6241 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggtcacgaac
     6301 ctatgggaat cgttgaagaa gtaggatcac aagttcaaac attgaaaaaa gggcaacgag
     6361 tggttatccc ttttaatatc agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     6421 gccaatgtga tgaaacaaat ccagatcagt tatatggcgg gcttttcggt tttggcaagt
     6481 tgaatggcaa tcattggggt gggcaagcag aatacctaag agtaccttat gcagactcta
     6541 cgtcgtttgt cgttccagat aataatcttc ctgatgaaaa agttttgttt ttatcagata
     6601 ttttgcctac agcttattgg agcgttgaaa atgcaggcgt aaaaaaaggc gatacagttg
     6661 tgattctagg atctggtcca gtcggtctat ttgctcaaaa attcgctgta atggctggag
     6721 cagagcgcgt gattgcagtg gatccagtac aacatcgttt agataaagcg gctaattaca
     6781 acggcgtgga aacttatttc ttagaagaca cagcacaagc tggaacggaa ttgtatgagt
     6841 tggctaaagg tggtgccgat gtcatcatcg attgtgtagg aatggatggg ttagaacctg
     6901 taaaagaaaa agcaaaaaat ttggtgagcc tacaaagcgg cacgatttct ccattacaga
     6961 tggcttcgca agctgtcaaa aaattcggaa ccgtccaagt aactggtgtg tatatgacgc
     7021 cagcttcttc ttacccgctt caagagtttt tcatgcgaga tattgcggtc aagcatggac
     7081 aggcaccaat tatccatctg atgccgaaaa tctacgaaat gattgcagaa ggactatttg
     7141 atcctacaca gatcattact cattcgctgc cactagaaga agcagcacaa gcatatgaga
     7201 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga aatcagtaaa
     7261 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     7321 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaaccgtt
     7381 acacattgat ctcactagct gtcgacttat taaccgggat tttctatatt tttggaagcc
     7441 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgctg
     7501 ttttcttgac gattcgtcct attctcagaa tcgttaggaa cattttcatt tacgacgaga
     7561 aaagaacaag gaaaaagcat gggaaggcag tttagccttc tccatgcttt tttgttattc
     7621 ttgtgttgtg catatttcgc atactgcgat tcgaaaaatt tctaagtata gaacaatttt
     7681 tcggtttcct tctctatata ctcacgtcta tgctgtttca tgtatctact cttacagcgt
     7741 catttggtac actcgcacag gaattccttc attatcctga gccatcccga taaactgcca
     7801 attgtatttt tcataaaaat ccgtatgatc cgtcacaagg taaagcacct ttacgcccat
     7861 ttctgcaaag tcttgcttga tcgtttctaa aatcattttg gcaatccgtt ggtttcggta
     7921 gtctttttca acaaacagcg cacagacatt cggagtcaaa tctactcgat catgaaaatc
     7981 attgtcaaca acacctgcac ctgcaataat ctctccccat tcattcaaca ccacatacca
     8041 ttgcggaacc gatgtgtttt ttctcgtgct tgcttggata ctttcttcat aaacagcaac
     8101 tggcagatgc cattttgaag caaaccattc ggctgtttct tttatcaatg tcggtcgatc
     8161 ccgcaatttt atgactgttc ttctctccat ttgtactctc tcccattcat atttggtcta
     8221 ttatagcacc ttgtatttca aatagctttc caatcgctcg atttttttga atgaatggga
     8281 ttttttattt atacacaaat ttcaagacaa atatacctcg attttctcat tcattattcg
     8341 atcctaagtt ttaaattact tatttccata aaaaaacaga accagataac cggttctgct
     8401 ttcttttttc attaagcatc caaagcttct tttcttcttt cttggatcgt agctaccttg
     8461 acgaacggca gatagatcaa aatagaaagg atcagattga cagcggccag tacaccacct
     8521 gcaatactct tcgttgccaa tacaccgcca ataactggtg gtgttaccca tggtggcata
     8581 acagtagctg ctggcactaa acctgtagaa gtcgcaaagt acgcaacact aactaagacc
     8641 attggtgtca aaataaatgg aatgaacatg attggattca atacgatcgg taaaccaaac
     8701 atcgttggtt cattgatatt gaaaacacct ggcgcaatcg acaagtttgt tacaaccata
     8761 tacggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa tgttgca
//
LOCUS       DAYYNG010000121         8815 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000121 DAYYNG010000000
VERSION     DAYYNG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.122"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..328
                     /locus_tag="ACHBFA_002211"
     CDS             29..328
                     /locus_tag="ACHBFA_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395644.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(425..859)
                     /locus_tag="ACHBFA_002212"
     CDS             complement(425..859)
                     /locus_tag="ACHBFA_002212"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9395645.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1091..1801)
                     /locus_tag="ACHBFA_002213"
     CDS             complement(1091..1801)
                     /locus_tag="ACHBFA_002213"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9395646.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1791..2651)
                     /locus_tag="ACHBFA_002214"
     CDS             complement(1791..2651)
                     /locus_tag="ACHBFA_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9395647.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2656..3294)
                     /locus_tag="ACHBFA_002215"
     CDS             complement(2656..3294)
                     /locus_tag="ACHBFA_002215"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9395648.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3309..3608)
                     /locus_tag="ACHBFA_002216"
     CDS             complement(3309..3608)
                     /locus_tag="ACHBFA_002216"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395649.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3651..5135)
                     /locus_tag="ACHBFA_002217"
     CDS             complement(3651..5135)
                     /locus_tag="ACHBFA_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9395650.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5155..5616)
                     /locus_tag="ACHBFA_002218"
     CDS             complement(5155..5616)
                     /locus_tag="ACHBFA_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395651.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5634..6701)
                     /gene="ulaG"
                     /locus_tag="ACHBFA_002219"
     CDS             complement(5634..6701)
                     /gene="ulaG"
                     /locus_tag="ACHBFA_002219"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9395652.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6919..7689
                     /locus_tag="ACHBFA_002220"
     CDS             6919..7689
                     /locus_tag="ACHBFA_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9395653.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7962..8789)
                     /locus_tag="ACHBFA_002221"
     CDS             complement(7962..8789)
                     /locus_tag="ACHBFA_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395654.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2672 a         1855 c         1388 g         2900 t
ORIGIN      
        1 atgtatcagt atagaaagga aataaaacat ggattatact gttgaatttg acagtgaact
       61 acttaagcaa tttccacaag aagaacattt catgtataaa gaagcaattt cccacaaagt
      121 gacagagtat ttggagcata atttttttcg tataaagcca gtgcgtactc gttcggcaaa
      181 aggaatatac gagatgaaag tcaaaattaa taaaaaaaat tatcgcatag catttgcttt
      241 aaaaaaaaat catgtgttca ttttttatat tagttcgaat ttgcaaaaaa ttgtatttga
      301 taaagaggtg agtaaaaaaa taaaatagct gagcattgga ggatagattg agtgatacta
      361 agtctatcct cttgtttttc taaagaacta agctagaatt ttttcttttg tacgttatta
      421 tatgttattt gtcattacca aaaagcacct tttttcttct agtatctatg acataagaaa
      481 taccacccat ttcctttact tgaacaaatc ctactgattc ataaaatttt tttgttttct
      541 cagtatcatc tgtcaataat tcgatttgga ctggtccata actagtgatc ttctccaaag
      601 cttcattcaa aagcactttg cctattcctt ggcgctgata cctcggatga atgaggatat
      661 cttgaataaa tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg ataatcttcc
      721 cttccccatt acgctttatc agagattcag attgttcaaa tgctctagct aaccttgcag
      781 tgtcttttgt gtaattcgtc caaccaacgc tcttatataa ttcagaaatc tcatcgatca
      841 taagggaatc aatattcatt tttttcttcc tttcttatac aattctccct ctaataatac
      901 agttctttga ctatttaatc tatcacatat aactctcctg taaagattct ctcgattcct
      961 taaaattcct ctttcctttt agaatgcata ctttttgata aataaaaagg ttgtgataaa
     1021 ttttcttcac aacctctgtt tctctctatc aagtgttatc ttttaatttt ctacccttac
     1081 cctagcatct ctattgcccg tagtatgcat actttccatg tttgcgaaaa taatgtttat
     1141 ctagtaaata ttgcgggatt tcacatatat tggggttgat cagctcgttt tctttggcca
     1201 tcagacaaat ttcatctaag atcaaactgt tttctactgc tttcataggt gaatcccccc
     1261 atgtgaacgg tccatgtcca tagacaagta ctccagggac ttcgtttgga tctaattttc
     1321 tttccttaaa cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct tctcttactt
     1381 cttctttcgt taattggcgt gtacaaggaa ccttaccata aaaagcatca gcgtgagtcg
     1441 tgccataagc tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc gtggaatgag
     1501 tatgagtaat agcagttata tcttcaaaag tttgatacaa aacaacatgt gtggggagat
     1561 ctgaagatgg ttttaagcta tcttcttcta ttatatttcc cgacaaatct gttaccacca
     1621 tttgatctgc ttgcatgcat tcatatctga caccgcttgg cttaatgacg atgactccta
     1681 gctcacggtc cacttcactc acattgcccc aagtcaattt gactaatccg tatcgcggca
     1741 atgccagatt tgcttcaagg acacgttgct tcattgctgc aattttttct tcacaagaca
     1801 tcatagccag cctccttcaa tttagaactt aagtagtctt ttgcctgctg gatttctctt
     1861 cgaaagtctt ggcttttttc actccacatt tcaatcagaa atgttccatc ataatctaga
     1921 cgtttcaaat gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa aggaacatca
     1981 cgaaactggc ctgaatattc ttttgttaca gggtaagtat cttttaagtg gatagcagtg
     2041 atataatcga tccctttctc aagttctcga gccggattat tttccggcca tgcggacaaa
     2101 ttgcctaggt ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt gatttctaaa
     2161 aattttgaaa tggagttcat aaatggatca tccatgattt ccacggaaag aatgatgcca
     2221 tatttagaag cttcctttac gctttctttt attccttcga taaagtcttc tctggaattc
     2281 attgatttct cttcataata aacatcgtat ccagctattt ggattacttt gacgcttaga
     2341 ttatgcgcca aatggattgc ttttttcatt agttgtttcg ctgctttttt cttttctgga
     2401 tcagcagaac caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg gatacgtaca
     2461 ccggagctga acatctcttc tctcagctca gctatctttt cttcagacca atctaatcgt
     2521 gctaaccgct catccgtctc atcaatcgac atctcgataa aatcgaagtc catttctttt
     2581 acaagggaaa aacgttcttt ccatgaaata tctttgggta acgctttttc ataaatccca
     2641 atttgagcca tttccttacc cccagatacg aatgatctca tctttgaagt tttgtgcggc
     2701 cttcatcgga tcatttgctg ctgtaatacc acgaccagtg ataaaggtat acacatcgat
     2761 cccttcaaat aagtgtaatg tgtgtggatt taatccacca gttacagaaa cattaaagcc
     2821 tagttcaatc agttttttga tcttcgataa atctttttca ccccaagttt caccagataa
     2881 cagcgcatca cggctttgat ggtagattac ctgccggata ccgatgttat gccaatccat
     2941 ggcttgttca aaagtccaat tgccgtagag ttctacttgc aattcaccta tttctttttg
     3001 tgctgcctcc atagttggaa tcgttgcaca acaaatgact gtcatccagt ctgctcctgc
     3061 ttttgcaaca ttactcgcca ctgttcctcc agcgtctgca catttcgtat cagcgactag
     3121 ttttttatct gggaacatac tgcgaataca gcgaatcgct ttttgtcctt cctgtaagca
     3181 aagaattgtg cccacttcaa taatatctac tacatcacca actttcataa catctcctaa
     3241 tgcatgttct aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc tcatcttagt
     3301 tccttccttt acttgttttt ttggtaccac tttgtttcag caaatttttc actgatttct
     3361 ttgactgaca tcacattttt gataccgata actgtaactc cttttttctc tgcctgagta
     3421 aacatattca aaaaattgac tggcgtaaaa actacatcgt attggccagc tgaacttttt
     3481 ccctctgaaa tggcacaatg atggattttg gtaattggta cgtttagttc tttcattgct
     3541 ttttccacac ttctcatcat catcaagctt gttcctgatc catttgcaca cgatactaaa
     3601 attctcatct taatccctcc attatgagtg ttgtctaatc agattttctt ttatactctc
     3661 tgcttttctg catatgtgtc atagtcttct acaatcaaaa aataaccatc aggatttttc
     3721 cgatattgta actgcggaat agccaataaa ccaatgacaa ctacggcaat tccagcataa
     3781 cctaggactt tcatgattac tgtaaacaat ggccaagctg ttgcccagtc gaacatcccg
     3841 atataaccac cgtatgttga catcccaatc catgtagcga ataatgctga accgcctact
     3901 tgtatgattc ctgaaagaaa cgggaacagg caagcggctt tgaatccacc tcggttattg
     3961 gcataaactg ctataacggc attatcaaag aacaacggaa tgaaaccggc tatgacgatc
     4021 gttggtgatt tcaataaaat cagtaaacca atcatcaaaa actgtcccaa agcaccaaac
     4081 aagaacccaa tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc tacgtcaatt
     4141 cctggaacag ctcctggtaa aatcgtgttt gagattcctt ggaaagattg agttagttca
     4201 tcgacgaatg tccgaacacc taattgcaag atcgccagat ataccgcaaa atttaaagaa
     4261 gtcgtcataa tgtagaataa gaaactctgc ccttcttgca tgaattgcgc ttgaatcaaa
     4321 taatctttgc ctagtacaat cagaatgatc ccaaagaaaa atagcatgag taacgaagta
     4381 gcaaccatat tctcattgaa aatcgacaag aatcctggta atttgatatc ttcaattttt
     4441 ttgtcttgct tgattgatct atttttcttt tcatcccgct ttttcatcca ctcagctaat
     4501 tttgcaaata taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc ggcaccttct
     4561 gtcaattcct gcgtgattcc tactgttaag ttagaagcta ctgcccaata acaacctaaa
     4621 atcagtccca taaaaatcaa tacttgtatc ctacctagat caggaaagca aaacagtaaa
     4681 atccaaaaag cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa aacggaacgc
     4741 agtttcgtat atttctgaaa acggactaat aagatattta aaacaaatgc aatcagtaat
     4801 aaaatcatca catctccaaa ggtacggcca aatgtctcca ttaatccagc atccacagca
     4861 ttttgtccaa aataaggatc tgtaaccatg gcatccaaat tgaaccgttc ctttagtcca
     4921 accaagattg gccgaaaatt attgaccaaa ccaccagagc caacagtcaa aataaagtaa
     4981 ccgaccgttg cctttaagaa tccagcgatg cattcatgaa gcgggcgttt cagcaaaata
     5041 tagcctaata acacgataaa accaatcagg tatgctggct gcgtcaagat atttgttgca
     5101 aaatacgtcc aaatgcttaa caaaaattcc cccatctttt ttctcctcta tcaattaatt
     5161 atattctgcc atcacaactt ggtaatcatc gatggagttc gtagagatga gacgctcaac
     5221 caatccatct gtcattaata attcggataa tttttggata ttttctagat gttcttctgg
     5281 atcttttgct gcgagtgtaa aaaacaaact agccgttttc tcctcattct ccccttcact
     5341 ttcaaaacgg attggctgtt tcaacttggt aaatgaaata gctgttccaa aaacaccatc
     5401 acttttttct gaagaatgcg gcattgctac ttgaggtgcg atcacaatat aaggaccgta
     5461 tttttctaca caatggatga tctcctccac atacgtttca tcaataattt ccttatcgat
     5521 caatacaaga cagctttcgg cgatagcttc acgccaatcc gtgatttctt tatctgggta
     5581 atgaatcaat tgatggtcaa aaaagtattt cagcaagaaa ctccccttct ttcttataaa
     5641 aacgacggaa acggtgtatc attttcaatc gagaagacat cttcaaatcc tcgataaaaa
     5701 ttaaactcaa gatcatcttt gtttgtcgga tagatgaatt tcccgcctac ttgccagata
     5761 taaggtttaa atccatactt caggcgatcc tttttgaatt tccaaatttc agtgatctct
     5821 tttggatcag ccataaaatt agaccaaata tcgtagtgga caggaatcac tacttttgca
     5881 ttcaatgact ccgccatgcg cagcatatca acagaagtaa ctttgtctgt tatccctcgt
     5941 gggttttcac cataggcccc taaacagaca tcgattttgt gttcattacc atgtttggca
     6001 aataaattgg agtaatgtga atctcctgca tgataaagat tccctcctga tgtttcgaat
     6061 aaatagttta ctgcaatttg atccatctcc tgaggcattt ttccttttaa tacctcttct
     6121 ggatcttcac aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac tacattgatg
     6181 tctttgattt tgactgcatc acctggcttt actgtaatcg ttttttcttc aggaattccc
     6241 catttttgcc acgtgcgaac cacttctttt ggcccaatga atgttgcttc aggacaattt
     6301 tgatgaactg ctgcggctgt atggatatct agatgatcgg aatggatatg tgtaacgatt
     6361 aaagcgtcta cattttttat cgcaaaagga tcgattacga aaggctgagt acgtaaatta
     6421 ggctgcatat tttgaacgcc actcatacgc atcatctgat ggcctttttt catcatgcca
     6481 ttcccatgac tacgttttcc cgtcccacac catagatcac ataaaatatt cgttccttca
     6541 tgacttttca gccaaattcc cgtacagccc agccaccaca tagcaaaagt tccaggatcc
     6601 actacttctg tttcgatttc ttcattcaag tatgtgcccc actcaggaaa tgtttctact
     6661 aaccactttt cttttgttac ttcattgaca gttttactca tttgcttgct ccccccatat
     6721 cgttgttcca acttacaagt aaatattaat gtgtatcagc atccatgaaa acccttttat
     6781 atgaccgttt gttttttata tgattttctt tcagtaatca atgattgttt cagtataata
     6841 aagacagata tgaatgttta aatgaacaac tagtcaaata aatccatgga tcaagatgtc
     6901 cctcaggagg caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg tcaaaaagaa
     6961 attttggaac tgcttcagca gactgaacaa ctatcaactt tcgaattagc tgaatccctt
     7021 gatgtatcta ttagtacgat acgaagagat ttaaatttat tagaagaaaa aaacgagatc
     7081 atacgcaatt acggttattg ttcttataat tacaaaaacc agactgattt tgatcaatct
     7141 ggtcctgaaa ggattaaaca agcgattgcc agagaagcta gtaaatacat taacgattat
     7201 gatacgctgt ttatcaattc tagctcgact gcgttgaaaa cactggattt tcttcaatct
     7261 aaccatttga cagtcattac aaacaacgtg aaaatcaatt actctccgca caaagataat
     7321 tgtagttata ttttaactgg tggagagatg cgctttccaa aagaagcact agtgggcgac
     7381 atcgcattaa atacgatttc ttcagtcaat gcagacattt gtatcattgg ctgtagcgga
     7441 gtcgatgtag tacatggggt cacaacaaag atattaaatg aatcaacgat caacgaaatg
     7501 atggtcaaca agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa aatcggactg
     7561 acatcaaagt tcaagattgc tgatatttca gtctttgatt atttgatcac tgataaattt
     7621 tgttctgaaa aagtattaga ggagatcaga aatacgggga taaaaaccat tcaagtagat
     7681 tcattttaag aaccaggacg taaatatgta aataggatta tcaaaaaaat gaataacaaa
     7741 tttatcgata aatgaaaaac acgctacgct caaattgata aaggaatccc aagatgtatc
     7801 catccaatcg ataccatcat aatctattgc ttcttttata accgttatat gttgcctaaa
     7861 taggaatcaa acaatatcga aaaaaggagg tatgacaaaa gtcacacctc cttttttcga
     7921 ataaacggta ttcaaatcag aacactgatt tcacaaccac tttatttcag gtcgttttca
     7981 atcaccttta ccgcgtaatc atttccgttg aaacgcgcta ttttttttgc gtccatatat
     8041 ctttctaaat aatcatcttc tcctaattgc ttatggcaag aagcaatttc ataaagcaag
     8101 tattccaatg cataagtcga tttatgtttc ttattatact caataccctt ctcacataat
     8161 tggattgctt tatcataatc ctcgatttta cggtaatagg tggcgatatc ataatataca
     8221 atgcagacca tcagattatc aaaatcagtc tcactcaata ttttaagtgt acgctccaca
     8281 taaactttcg caaaatctaa ttctcctttt gacaagtaga tcattgccac gtttgcagct
     8341 gcttgtaagt gatggatatc tgtttcggtc gtttgttcca acacttgatt aaaataaaat
     8401 aatgctgcat ctggtttttt cgcataaaga tgatcaagca gtccttgata aaataaaatt
     8461 tgatttaaaa gatcagaatt acttactttt tcctgatcga tcgtttgtaa aattttataa
     8521 gattcttggt attttgatac agaattcaat gattgaatct catttatttt tttcctgatt
     8581 ttgtcttctt ctgtttctac taatatatcg gagatctcga cattcagctt tttgcaaagc
     8641 gcacttaaga tattgatgcc tggcgacata ttcttgtttt cgatattgct gacagttgct
     8701 tgttcacaaa tgccttctgc taattgtact tgtgttaacc cctttttctt tcggtattct
     8761 tttagtaatg ctccatctac tttaatcaaa acaatttcct tccctttaaa aaatt
//
LOCUS       DAYYNG010000122         8755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000122 DAYYNG010000000
VERSION     DAYYNG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.123"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..464
                     /locus_tag="ACHBFA_002222"
     CDS             282..464
                     /locus_tag="ACHBFA_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395655.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            448..633
                     /gene="tnpB"
                     /locus_tag="ACHBFA_002223"
     CDS             448..633
                     /gene="tnpB"
                     /locus_tag="ACHBFA_002223"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9395656.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            876..2396
                     /locus_tag="ACHBFA_002224"
     CDS             876..2396
                     /locus_tag="ACHBFA_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9395657.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKAPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2583..3155)
                     /gene="hxlB"
                     /locus_tag="ACHBFA_002225"
     CDS             complement(2583..3155)
                     /gene="hxlB"
                     /locus_tag="ACHBFA_002225"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFM9395658.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(3162..3824)
                     /gene="rpe"
                     /locus_tag="ACHBFA_002226"
     CDS             complement(3162..3824)
                     /gene="rpe"
                     /locus_tag="ACHBFA_002226"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9395659.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3840..4355)
                     /locus_tag="ACHBFA_002227"
     CDS             complement(3840..4355)
                     /locus_tag="ACHBFA_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFM9395660.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4357..4641)
                     /locus_tag="ACHBFA_002228"
     CDS             complement(4357..4641)
                     /locus_tag="ACHBFA_002228"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395661.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4675..6003)
                     /locus_tag="ACHBFA_002229"
     CDS             complement(4675..6003)
                     /locus_tag="ACHBFA_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9395662.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(6017..6472)
                     /locus_tag="ACHBFA_002230"
     CDS             complement(6017..6472)
                     /locus_tag="ACHBFA_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395663.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6475..8421)
                     /locus_tag="ACHBFA_002231"
     CDS             complement(6475..8421)
                     /locus_tag="ACHBFA_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395664.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2919 a         1626 c         1373 g         2837 t
ORIGIN      
        1 ttattgaaag attcgattaa attagttgag taaatgcttc gtacgaacat agttgattta
       61 ttcaccatac ttcccattac atcgtaaaac gctatattta agatttcaaa caactatatt
      121 aataggaaga aaacttcatt tttcctctta gttctataaa aagtgtggct aatttgaggc
      181 aaaatattaa attaaatgta agcgtgtcat aggatagtat ctcgtacgcc gattatcttt
      241 cttctataat taaactatca aatatagaag gagaaaaacg aatgaataaa aaagatattg
      301 taccagttca attttcaagt gcggctcaaa aatcgcaaat tgacaccaag ccaaaactag
      361 cttgcagaat caaatcggaa gataaggaaa tatttattta taatggaatg gataaatatc
      421 ttttgtatac cttgttaaaa gagttgaatg ccaatgctca ttgatgcaac tgcattgaaa
      481 catattttta tcgtttgtgg aaaaacagat cttcgaaaag gaattgatgg actagcagct
      541 ttcgttatag aagaatatga ccttgatgtt tacgatcaag cgcttttctt attttgcggt
      601 acgaagaaag atcgcttcaa ggctctttac taggatcaaa atggatttgt attaatgtac
      661 aaaagatttg aaagtgggta ccttcaatgg ccgcgttcca gaaaagaagt cttactgact
      721 ggcctttcaa tcgaagaaaa atgtacgcta aaagaaacga aaaaaggtgc gatatattag
      781 acgaaaacct tgttaaatca aggattttgt ctgtttttcg ttgtcataaa atggtataat
      841 agatgtaaga aaatcgctag aatggtggtg taaaaatgac aaaaatggaa tctgaacttc
      901 ttgtacaaat caagcaatta acacagttgc ttgaaatcca gcaacaggaa aatactcttc
      961 ttagagaaca aattgctgaa atgaatcgac gcttgtttgg acgaaaaaaa gaaacaccac
     1021 ccgtagatgg gcaaatcgat ttattagacg actcaacttt taatgaacca gagcacactg
     1081 gacaagaaag ccaggaaccc attacggtat cttcttttaa acgcagaaaa cgcaagggac
     1141 tcaaagcact ttcattagaa ggtctgcctg aagttcctat tcattatgaa ttaaaaggtg
     1201 ccgattgttt gtgtgaagac tgtggtcata ctttacatga tatcggtgcg acaaggctta
     1261 gagaagagcc attgtatgtt cctgcccata tagaaaaaca agtcatctac caacatgcct
     1321 atcattgcag agaatgtgaa gcattaggag aaacaaaaat caaaaaagcg cctgttccta
     1381 aaccgttgat caataatagt ctaggttcct cttcaattgt gacacagagt atttgggaaa
     1441 aatttgttaa aaaggcgccg gcatatcgac aagaaaaaga atggcatgca cttggtttcc
     1501 ctcttgatcg taaaaaaatc actaattggc atatcaaggt aagtgaaatg gttttagagc
     1561 caatctacaa ttgccttcat caagaattaa gcaatcagga ggtcttacac gcggatgaga
     1621 caagctatcg ggtattgaat actcataaag aaaagacctt ttactggtta tatgcttcag
     1681 gtaaaagcga agaaaaacaa gttgtgctct acgagcacgc aaattcgcgt tccgttgatg
     1741 agccaaaaag ttttttagaa tcatttagcg gtttcttgca tactgatggt tacgcggcct
     1801 atggaaagct gccggtaaac aatgtttatt gttgggcgca tgttcgcaga aagttttttg
     1861 aagcattggg taaacaaccg tccccaaaaa gtcatgccaa aataggatta gacttctgtg
     1921 acaagatgtt tcgaatcgaa cggaccttta ataagcttag cgcagaagaa aggtttgtag
     1981 cgaggaagga aacgttgcag cctgtgatga ctgaattttt tcattggtgt gagagttttg
     2041 cttatttgcc aaaatcgcag cttggaaagg cggttcaata cgcagtcaat cataaagacg
     2101 gacttcagat tgtgttgctt gatggacgct tggaactttc aaataaccga gcagagcgtg
     2161 ccatcaaaga gttagtgatc ggtcgaaaaa actggctatt ttctaaaagc ttaaaaggcg
     2221 ctagatcaaa tggaattata ttgagtatta tccagacagc tgttgcgaat gggttaaaca
     2281 tccgcaaata tttaaatcat ctttttacag agattcccaa tctgtcttcg atgacacctg
     2341 aagcgctacg cgcttatttg ccatggaatc agcagataca agaaatctgt aaataatata
     2401 agccacaaat aacctgattt gagtgtatca aatcgggcta tttgtgttta ggtaccattc
     2461 tatgacacgc ttacgctcaa aactactgaa ttattaagtt atgtctaatc aaataaaaaa
     2521 accattctca tatttgctga ataattagca tttagagaat ggttttttat ctttaaaatc
     2581 tactactcaa aatctgcatg tcgagcaaac atcgtttctg agctttcacc tgttttatcc
     2641 attaaagata acactaaact atcaaaggtg agaaatgcta attgttcaaa acttgaaccc
     2701 ataggttgtg aaaaagtatc ttcttctcgt tgattttcat ctttggtaat acctggtaat
     2761 acaagaacag aatctgctaa ttgaccaatc gacgagtctt ttttcatggt aactaaagca
     2821 atgcttacac cactttgatt tgctttggcc gctaaatttt ttaaactacc tgtttcacct
     2881 gagccagaac agataattaa taaatctcca ggttgcgaat gtggtgaact aatttcaccg
     2941 acaaggctga ctgaaaaacc taaatgcatt aagcgattcg taaatgcttg catggcaatt
     3001 ccagagcgtc ctgcaccagc tagaaagatg tgtttagctt gaacaatttt ctcactaaat
     3061 tgttctaact ctacagaatt aatacgtttt gagttatttt gtaattctaa gataatcgct
     3121 tctaaatagt ttttattttg gtctttattg gtcatgatct tttacttcac ttcctctaat
     3181 gattgattta gtttatcaat ttgttcttgc ggggtatttt tcccattgaa gatgaaaccg
     3241 cctgatacaa aaatatccgc ccctgcttta gaacaaactt taatcgtttg attatcaatc
     3301 ttgccatcta cttcaatatc atacgtataa tcattttgtt tgcgtattac atctaactgt
     3361 tcgattttaa ctactgtctc ttgaataaaa tgcttattct ctgttcctgg attgattgtc
     3421 attactaata cttgatcgac taaagataat actggttcaa taaaactgac tggtgttgca
     3481 ggattaaaag taatccctac ttttgctcct gcttccttga ctaactgaat gaccttcatt
     3541 gcgtgtttgg tagcttcaat gtggaaggta acaatatctg ctcctgcttc tacaaatctt
     3601 gtgacaattt tttctggtgt atcaatcatc aaatgaacat caaacacaac gtctgttagt
     3661 ggacgaatat ctttaataac acttggtcca aaagccatat ctggtacaaa acagccatcc
     3721 attacatcaa tatgcaaata atcaacattc ttttctttta agatttcaaa taactcttta
     3781 acttctgcga taggtgcatt taacaatgat ggagcaattg tcattctcta ttcccccgtt
     3841 tattctccca taatcatgag attgattgtt ctagttcgct ctcttagttg atcccaatcg
     3901 acaaaataga cacggcccat ttctcctaat aaaagtttac cttcttgaac aattaaagtc
     3961 attgagttgg ttccataaat agatgaacgt aaatgtgcat cggtatttag catgacccat
     4021 ttttctgcag ggtaattggg atcagataat cttgttccaa aatcaatgtg ttttggcccc
     4081 ggactgttgt attgattttc actcgtcatt tttggaataa tcgtttccat tacattgttg
     4141 atgtctacat gtaaataatc atcaccaaaa aagtttgtgt catgcatcgt ttcttcaaaa
     4201 aagagtgagc aagttgtatg cgggctcgaa attactaaca ttccattctc aatacctgct
     4261 gtatcaactg tatctaaaag ttcttttcga atatcatgat acgaaggacg ccctgaaact
     4321 gtttcaatag tcaaagtgtc atgatgaaaa cgcatattaa acacctaatc tttcttttac
     4381 atctgcataa atggcatctg taccaatacc tgttaataag cccaagccat tcgttacttt
     4441 agctttaatt acatcaggga caattgttgt acttactaca gcatcatatt catcaatttt
     4501 atttagctca tcagcaattt tagattgata aattttcact tgatctgtta atccttcttc
     4561 ttccaaccat tttgataatt ttgctactgc aattgtagaa gtagcatgac ctgctccaca
     4621 aataactaat aattttttca tattgatctc ttctttctta ttttaatttt atgattaagc
     4681 tttcttcact ttgttcatgt agatagaagc acctactaaa acaactccta atccacctat
     4741 tccaattaca ccaaagtaag atgctaataa aacaaacaac cctactggcc ataatccaca
     4801 taacaacata gttactaatc ctgctgtccc cacatcgaaa cttgctaatg taaacacatc
     4861 agtgataatt ggtgccaacc atgttgccat atataatgtt gtaacaacat acaacgaaca
     4921 tcctactaat gatctcacaa tatttccttt aaaataagga atcattaaac agatggcgaa
     4981 agcaaagaat gctaaatctc caaaaggtaa tactttattt cctggaagaa ttagtgctag
     5041 caataatgag attggtacaa gcaatagaga agttgacata atagttggat gacccacagt
     5101 taacgctgca tccattccaa tgtttacttg gcgaccattt aactttttat tcgtaaattc
     5161 tttcgccgct tctgcaattg gcattaaacc ttccataaac attgctacca ttttaggcat
     5221 aatcttcatc actgccgcag tcataattcc taagtttcca attcctgcta cgtcatagcc
     5281 tgccataata cccactacaa taccgataat taggccaata acaactgtgt cacctaaaac
     5341 accgaaacgt tttgttaatg tttctgcatc tgcatctatt ttattaaatc caggaatacg
     5401 atcaaaaacc caatttactg gtttggcaaa tagtactcct gataatccca tcatatgtgt
     5461 gatagagatt cctggatagc cgaaaaattc gctaacagtt ttttgtgtac gatctgctaa
     5521 gaaccattgt aaaattactg cgacaatcgc gccaattaag ccaaaccaaa agtttcccgt
     5581 aacagattga gtaatcatac ctaggaaaat tgcgaaccaa aagttccaaa tatcaacatt
     5641 caatgttttt gtccaaccca aaaccacaaa tatgaagtta agaattaatg aaattggaat
     5701 cattaaaatt cccaatgatg tagagtaagc caaaggtcca ccaacaccta cgccaatatc
     5761 taggatattc aggccaccac cgtattgttc ccccatttgt tgaattgctg ttcctaaacc
     5821 atctgataat aaacttaaaa ctaatcctaa accaatagaa ccgataccaa cagtaacgcc
     5881 ggaaacaaat gctttaccag gttttaatcc aaataatatc ccgattacaa aaataatcag
     5941 tggtaaaaag acgtttgacc ctaatccaat aaaccattca aaggcattta atacaatatc
     6001 catctctcta ttcctcctaa taaactcctg cagaatgtac taactcaata aatgcttctt
     6061 tcgtggtaca tgcaagtaat tcatttactt tttcttcatt ttgaaatagg ctaactaagt
     6121 tttgtagtgt atcaatttgt tcatgaggtt gtgacattgc caacataaag actaaacgta
     6181 caggtatttc atgagattta tctgtcatat ctgaaaatac cagtggttcc tttaatgagg
     6241 caaaacagat ttgagagcta tttacatgtg cggaatctgt atgtgggata gcaaacccaa
     6301 tttgatttac ttctaatcca gttggaaatt cttgttcacg gttggtgata ccttctaaga
     6361 aactatcttt cacgcaacca gcgtttttta attgttcaga cattacttga aacaattctt
     6421 tttgatcttt cacttctaat tgaaataaac ataattcttt atctagatac atttttattc
     6481 tcctttttgt tgaaaaattc ttagtaattt ttcgctatca tgaacttgta acaagcgatc
     6541 gaccatttct ttttgcccaa taaattggta aagttcttca tatactcctc taatctcaga
     6601 tacatcttct tcagctaaac taaccataat gactaatttg acttttcgat cgccccaatt
     6661 aatcgcattt tttaatgcta caataaaaat gttggattca atgaccattg atggattcgc
     6721 atgcggtaaa gcaacaccac tttctaaatt agtattcccc attttctctc tagagaagat
     6781 agaagcacga aactcttcag taactaatga attggcttct agttcggaaa tcatcaaatc
     6841 caaacattct tctttgcttt ccacctgtac tcctgttttt actaaagatg attgaacaaa
     6901 tttagatatg gtgggaactt taaccgcttt aggaatcagt tcattttgaa taaatcgcga
     6961 aggttcttct attaaatatt tagtataagc ttccataata ttcatataat ctgttttact
     7021 taccaatgga gataccctta ctaagggtat accacctgca tcaaaggaaa gagaggaaat
     7081 aatcaaatca acttccccaa tttcagctaa atgaaactta tctaaagttg atatttctac
     7141 attatctttt gctggtaaaa accgtcgaac tttattgtaa attaattgcg cagaagaaat
     7201 accgtattgg caaataatga tgatattatt tgctttggac tgatcttcaa tagcaatctg
     7261 aaaatgaatc aaaattaagg aaacttcatc gtccgttaaa gtcacatcaa attcttcttc
     7321 aaggatggac atggcgtacc atactatgct aaagagttct gaatattgag atttgatatt
     7381 ttctaataac ggattttgaa ttttaatacc tttttttaaa cgaaggacca ttgctggcac
     7441 atgatacaat aaggattgat ataatcgatc attttgagtc aaatcaattt tttcaatctc
     7501 actcattctt tcaatcaatt tatgaacgct catagcgtac tcttcattgc tggtttttaa
     7561 attatttgtc acacgatgtg caaatagttg acgacataga tattctttat ctgcgtcata
     7621 aaatggaaga ttcaattttg ccttcaattc ttcaattaaa ccatttgcca caatgtacgt
     7681 ttccatatag cgaatgctat tgaagaggaa ttcttcttca acagttaaat gatgaccttt
     7741 cagtaaacgg ttacattgga taactaaagt agaaatcaat gaccgaacat agtaatcaga
     7801 aaccttttca gtcagttcac tgtaatcatt gaatagcaaa tcatgaatga cactaataac
     7861 ttttgaatca aacaacatat ttaaaacttg aaaaaatgtt tttgattcca tgtcattcgc
     7921 ataatagaat acaagttgct tgattgctcg ttgaattgct tgttcattac cagtaaaact
     7981 aattccttct gcagatgact gcaacttaac ttctccactg ccaataattt tattgataat
     8041 tctcaggtca ttgtataacg atgttttact aaccataaaa ctttgagaca aatgagttaa
     8101 agaaacaggc ttatcaccaa acaaaacttc tttaatgatc tcgacacgtc ttttttctgg
     8161 agaataaata tctttttcaa gataattttc taccgatgaa atcaaatgtt caacgttttt
     8221 cctatcacct atgagtttga tacccaaaga aggtaatcgc tcaattgtta cttggtaatt
     8281 aactacataa tcttccaaca attcaatatc ttgataaatg gtcttggtcg acactgccaa
     8341 tttatcacta aaaaaagaag cgggtttaaa gtcttcttgt cgacttaata attttaataa
     8401 atttacatgt ctttcaatca aacttgttca cctcttaacg ctagcatttg ttacctcttt
     8461 tcattattta ttctagcact ggaaccccct tccctcaatt acatctatgt caataatact
     8521 tgtggaagtt tgttgtaaag gaaatccaaa taatcttaaa aaatgagaaa caaaaaagga
     8581 gaaatcctta aaagagctaa gatattccaa tagaaaatac tccttattat ggaagtaata
     8641 tgaaatatta caattgaaaa ataaaaacaa tataatggcg tcactaaaat tcgtattttt
     8701 ttattataat ataacttaat tacaaatgct catctatgaa caattgagtc ctaat
//
LOCUS       DAYYNG010000123         8716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000123 DAYYNG010000000
VERSION     DAYYNG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.124"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(276..1160)
                     /gene="ftsX"
                     /locus_tag="ACHBFA_002232"
     CDS             complement(276..1160)
                     /gene="ftsX"
                     /locus_tag="ACHBFA_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9395665.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(1153..1839)
                     /gene="ftsE"
                     /locus_tag="ACHBFA_002233"
     CDS             complement(1153..1839)
                     /gene="ftsE"
                     /locus_tag="ACHBFA_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9395666.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1944..3045)
                     /gene="prfB"
                     /locus_tag="ACHBFA_002234"
     CDS             complement(join(1944..2972,2974..3045))
                     /gene="prfB"
                     /locus_tag="ACHBFA_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9395667.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(3171..5705)
                     /gene="secA"
                     /locus_tag="ACHBFA_002235"
     CDS             complement(3171..5705)
                     /gene="secA"
                     /locus_tag="ACHBFA_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9395668.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPYPCGSGKKYKNCHGKNAN"
     gene            complement(5927..6478)
                     /gene="raiA"
                     /locus_tag="ACHBFA_002236"
     CDS             complement(5927..6478)
                     /gene="raiA"
                     /locus_tag="ACHBFA_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9395669.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6606..7325)
                     /locus_tag="ACHBFA_002237"
     CDS             complement(6606..7325)
                     /locus_tag="ACHBFA_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM9395670.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7322..8635)
                     /locus_tag="ACHBFA_002238"
     CDS             complement(7322..8635)
                     /locus_tag="ACHBFA_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9395671.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
BASE COUNT         2322 a         1905 c         1530 g         2959 t
ORIGIN      
        1 tcaaaacgag cagccatgcc catgtttgag cagtttcctt tctttctcga aaaacgatga
       61 taaaggaaag caaaatattc ataatcaaaa ggatcgtaaa aaaattgtca acgataattt
      121 tcacggcttc acctcttctt ctaccaactt aagaataccc aagttgtttt ttaaagtaaa
      181 gggaaaaaag ccgcttcctt ctattctcct agccaataaa aaaagaaagc tggaacgaaa
      241 ctgtccaact ttctcatttt atttatattg tggtattaga atttcaaaaa acgtctcata
      301 gaaatgatcg agccaagtga accaatgatc acaccaatgc cgaccattaa aagattgatt
      361 ttccaaacga tattttgtgg acgaatcaat gaataatggg aacgtaataa tgatggattg
      421 atcaattgga aaaaccatgc ataaccaaat gtgatgatca aaatcggaag aatcgcaccg
      481 atcagaccga tccagccacc ttcaaggaaa aacggccagc gaatatatcc attttttgca
      541 ccgactaaac gcatgatttg aatttcacgc tgtctagaca aaatcgtgat ccgaatcgtg
      601 ttcgaaatga gaaacatcgc tacgaacagc agcaatgccg ctgcaactaa tccccatgtc
      661 cgaactgcac cagcgatttg gaaaatccga tctgaggaag ctccaccgta atcagaacga
      721 gaaacattct tcaaatctgc tgcttcttct gctacttgtt ttgtataaga aggatcagta
      781 gcacttacta catacacatc atataacggg ttattgtcac cttcaaacaa tttccaggaa
      841 tcacccattt cgttttggat ttttttcaat tcctgttctt tgcttgaaaa ttcaactttt
      901 ttaacatgat ctaagtcatt caattctttt tctagctttt tcatctcttc gtcttttgta
      961 ccgatatcta cgaatacaga tacatcaacg tttccttcta tatcctgagc taatttagtt
     1021 gcattcatga taactgccat gaatacaccg actaaagtca acgtgatcgt taccgcactg
     1081 actgaagcaa ctgtcatcca gccgttacgt ttcaaactct tgatactttc taataaatgg
     1141 cggaaaaagg ttctaatcat cgtatccata ttccccttcc gcttgatctc gaataatgcg
     1201 gccgttttcg atagcaatta ctcggtgccg aatcgtattt acgatcgtac tgttatgtgt
     1261 agccatcacg acagtagttc cttgggcatt gatccgatct aataatttca tgatttccca
     1321 tgagttttct ggatccagat ttcctgtcgg ttcatccgcg ataagtactt taggcgtatt
     1381 cacgattgcc cgagcaatag agacacgttg ctgttcccca ccggataatt cgctaggaaa
     1441 gactcgaact ttgtgtttca aaccaacaag gtccagcact tccattactc gttttttgat
     1501 atctcgaggt tttctgccga taacttgcat agcgtacgca acattttcat atactgtttt
     1561 tcttggtaat aatttatagt cttggaacac cacgccgatt tctctgcgta gataaggaac
     1621 ttcgcggttt ttgattgcta ttagatcatg acctgcgacg ttcaaagttc cttttgaagc
     1681 tttttcttct cgatacatca atttgatgaa tgtagattta ccggctccag acggaccaac
     1741 cacatagaca aactctcctt gttcgatgac aatcgagaga ttgcggatag cggtcgtacc
     1801 atttgagtac ttcttcatta catccttcat ttcaatcatg gcgttctctc catatctttt
     1861 tcagtcttta gctattatag catggcaatg tttcaacatg cttgcaaaat cttacaattt
     1921 tatttcaatt taacaatgag ttcttattgt agatgcattt ttaaatagcc gtcaataaat
     1981 aaatcaaggt caccgtccat aactgcttgg acatttcctg tctctacgtt tgtccgatgg
     2041 tcttttacca tagaataggg atggaaaaca taagaacgga tctgtgaacc ccagccgatt
     2101 tctttttgtt ctcctcgaag tgcagctgct tcttgttctt tcttttccac ttctaattga
     2161 taaagcttag cttttaacat ccccatcgct tgctcacggt ttttcaattg tgagcgttgt
     2221 gcctgactag ctactactgt tcctgtaggc aagtgagtga tccgtacagc agattctgtt
     2281 ttattgatgt gctgaccacc tgcaccactt gctcggtacg tatcgatttt caaatcatct
     2341 ggattgattt ctatatcgat cgtttcatca agctctggca tcacatctac cgaacaaaaa
     2401 gaagtatgac gtcgtttcgc tgagtcaaaa ggcgaaatcc gtacaaggcg atgaacacct
     2461 ttttctgatt ttagataacc aaaagcgtta tgtcctttaa tcagcagggt cacactcttg
     2521 atccctgctt catcacctgc ttgataatct agtgtttcta cttgatagcc atgctgctct
     2581 gcccaacgtg tatacatacg taaaagcatg ctgccccagt cttgggactc cgttccgcca
     2641 gcccctggat gcaattcgac gatcgcattg tttcgatcat aaggtccgtc taacagcata
     2701 gataattcat acgtcttcat tttttcatct aatgcattga tacgtgtttc tgcttcttct
     2761 tgcattgctg catcaggttc ttcttgcagc atctccacca tcaattcgat ttcttctagt
     2821 tcttcggcaa gagaatggaa ttggtcatag gtttctttat tagcgttatt ttcgctgatc
     2881 actttttgtg cggcttcgct gtcatcccaa aaaccaggtt cagccatttt tgcttctgct
     2941 tccgcgatgt cttcttccag ctgatctaag tcaaagagac ctcctgaaac ttgtgacctt
     3001 ttcctgcatt tctgtcagga gatttcgaat ttcggatatt tccatgtttt ttccttcttt
     3061 cactatactt ttttcattat atctagtcta acagaatcag caaataacag aaagaaggtg
     3121 agacgaattc ttaaaaaaag aatttgtctc accaatcctg ctgtcagaaa ttagtttgca
     3181 ttttttccat gacagttctt atattttttc ccgcttccac aaggataagg atcatttctt
     3241 ccgaccttgt tcacatggat tggctgtttt ttcgcgctag tattactatt ttctttggca
     3301 tctccgtctt ctgagtgaga agctgttcct tgagaaacct gttcacgctg tacgttttga
     3361 cggatctcag ctttcataaa gattcgagtg acttcatact cgatggcgcc taccatatct
     3421 tcaaacatct tatagccttc tgtttgatat tcgactaatg gattgttctg tccataagca
     3481 cgtaagccga ttgactgacg taattgatcc attgcatcaa tatggtctgt ccatttagag
     3541 tccacaacac gcaagatcac gactttttca aattccaaca gttgctctgg accatttagt
     3601 tgagccgctt tggtattaaa tacttcttgt gctcgttcgt ttagatactc tttgatctcc
     3661 tgagatgttt ttccttcgat atctgcaaga gtaatactat cttcatggac aagagtactg
     3721 ccggcaaaat cgatgatact ttccaaattc caattttctt tctctaattg tgtatgcgca
     3781 tctactacac gactgattgt ccgtttgacc atgttcatta gtggcttgga taagcttttt
     3841 tcttccatga ttacttcttg tcgctgttta tagattactt cacgttgttc acgcatcaca
     3901 tcatcgtatt gtaagacgtt tttacgtgtg tcatagttat ttccttctac acgtttttgt
     3961 gcagattcta cttgtttcgt cagcatcttg ctttggataa cagcatcttc ctcaccgatc
     4021 ttcatccgat ctaagaaagc tttgattcgt tctgaaccga atcgtttcat caaatcatct
     4081 tccaatgaca agtagaattg tgacatacca gggtctcctt gacgtcctgc acgtcctcga
     4141 agctggttat cgatacgacg agattcatgt cgttctgtcc cgataactgc tagaccacca
     4201 agttctctta ccccaagtcc taacttgata tctgttcccc gaccagccat gttggtagca
     4261 atcgtcacag cacctttttg accagcattc atgatgatct ctgcttcttt aaagtgattt
     4321 ttcgcattca gcacttcatg aggaatacgt tcttgattca gcatatctga taataactca
     4381 gatgtttcaa ccgcaacagt tccgactaac acaggttgac ctttgcggta gcgatctttg
     4441 atatcttgca cgacagcgtg gaacttgctt tgtaatgttg ggtaaagtaa atctggacga
     4501 tcatcacgga taaccggacg atttgtcgga atctggtata cttggatgtt ataaatttct
     4561 ctgaattctt cttcttctgt tttagctgta ccagtcattc ccgcaagttt cttgtacata
     4621 cggaaatagt tctggaaagt gatcgtcgcc atggttttcg tttcgtcttc gatctctacg
     4681 ccttctttag cttcgattgc ttggtgaagt ccatctgaat aacggcgtcc atccatgatc
     4741 cgaccagtga actggtcaac aatcagtact tttccgtctt ggacaacata atcgatatct
     4801 aaaagcatga tatagttggc tctcaatgct tgatccaagt gatgggtcaa tgcagtattt
     4861 tcaatgtcgt ataagttatc caaaccgaat gtttcttctg ctttttcgat ccctgcttcg
     4921 gttaaactga ttgtttttga ttgtacatcg attttataat cttcttcttc tttcaaacgt
     4981 ttcacgaagt tgtctgctct tgtatataaa gctgtggatt tttctgcttg ccctgaaatg
     5041 atcagcggcg ttctcgcttc atcaatcaag attgagtcaa cttcatcgac aatggcataa
     5101 ttcagcggac gttgtaccat ttgatggcgg taaacgacca tattgtctct caagtagtca
     5161 aatcctaatt cattattcgt actataagtg atatcacaat tataagcctc gcgtttttca
     5221 tccgaagatt ttgagttgat attcaacccc actgttaatc ctaagaaatt ataaagttcg
     5281 cccatttccg tcgagtcacg ggttgctaaa tattcattga cggtaactac gtgtacccct
     5341 tcgccagtca gagcatttag atatactggc atcgttgcgg tcaaggtttt accttcacca
     5401 gtacgcattt cagggatatt tccatcgtga agcacgattc cacccatcaa ttgtacgtgg
     5461 tatggatata accccaatac acgttttgct gcttctcgga cgactgcaaa agcttccgga
     5521 agtaattgat ctaatgtttc tcctttttgg tatctgccct tgaattcagc agtttttgcc
     5581 tgtaattgtt catcagttaa tgctgccatt tggtcagcgt atgcttcaac tttttttgca
     5641 atgctatcta aacgttttag ttctttctta tcgttttcga taagtgaacg aatcaaattg
     5701 gccatgcgtt gtgtaatctc cttttccttc tgtgtattat ccagactatg tagactttac
     5761 ttttcatttt atcattagtt atagataatt gaaatatctt aagcgtatta aagtaagaac
     5821 ttcataattt attcatattt catctaaaag aggaggaaac tcaaaaaaac ttgcggcagc
     5881 tgtcagtagc tgctgcaagt tttcgttgtt atttaagtaa ggctgatcag tctgtttcaa
     5941 tcagtccgta ttttccatct ttacggcggt aaacgatact tgtaccattt gtttctgcat
     6001 cttcaaaaat aaagaagtta tgtcccagca tgttcatttg taagactgct tcttcactat
     6061 ccattggttt caatgaaagg cgtttcgtac gtacgatatc taattcagaa tcattttcgt
     6121 tttcgtcctc ttcattgaat aaagcagcag catctgcagt attcatgccc gtttcacggg
     6181 atttacgatt aatttttgtt ttgaatttgc ggatttggcg ttccaattta tcaacaacca
     6241 aatcgatact tgcgtataaa tctggagaag tttcttcggc acgtaataca agataaggta
     6301 atgggatcgt tacttccact tttgctgttt tttctgtata aacttttaag ttcacatgcg
     6361 ccgttgcatc gggtgcgtca ctgaaatagc gttctagttt gcctactttt ttctcaacgt
     6421 agtcgcgaat cgcttcagtt acttcgatat tttcgccgcg tacattatat ctaaacataa
     6481 aaattacccc tttcgtctac cacaatcaag aaggagaagg accactcttt ccctcttctt
     6541 aattctatta tatcgaactc taggttattt gcaaattaaa tcgctatcaa atttccacat
     6601 ctaattcatc tcgccaaaga aatggttcgg atgattttcg gctgtgcgac ctgcaaacaa
     6661 tctgctgcgt ggaaaagagt ctgtcctgtt gtatagacat catcaataat caatattttt
     6721 ttattttgta tcttagaaaa ggtgactgca agttcaaaag gttgaggcat ctccaatctt
     6781 tcctttcgcg tgttttttgc ttgagccagc agatgcttct ctcttttgag tagcatctga
     6841 aaaggaattc ttgctgcttt caacatctcg cttacttgat tgaatcctct ttctgttcgc
     6901 cttttttcag ataaaggtat tggacaatag atgtatgtcg ggtattcttt ttttatctgc
     6961 gtccatttat atgcaaaggt gcctgctagt cggatatctc ccataaactt gtattgattg
     7021 agccaggcct tcatattttc atcgtacaca aaaaaggcac ggtgctgaaa atcataggtc
     7081 ggatatttct tcttccattc ttgacagtca ctgcagtaaa cgggaataga ctttactttc
     7141 tttttcgtac tctctagatt gaaatggatt ggtttttgac agccagaaca gcctcttgtt
     7201 tccaataaat gaaattgtgc gcgacaaact gaacaagtct gaggtaatct gtatttcgaa
     7261 acaccaataa tctcgctgaa tgttaaattt tggaagatat ctttactgca gcaactgcac
     7321 ctcacagcat cttcttccct tcttcattca tttctttgat ttcctgacaa gcttttcgaa
     7381 tagctgcagt tttttcttca tagaaaaagt atacttgact gttcgtataa ggccctttac
     7441 gatcggctct tcctgcgatt tgtaccaaag aagatttagt aaaaaccgga tgatccgctc
     7501 ctaaaacgat aacggatatt ctttcaaatg tgactccacg ttcaaggatc atcgtggtta
     7561 acagtatatc atattttgct tctcgcattt tctggacttt ttccgcccgc tgattgtctc
     7621 ttgaagaaac atctgtgatc tcatatctcg gaagagcctc ttgaagtgtg gctttcaaac
     7681 gatgcatcat cgtgatactt ggacaaaata aaaggatatg gttttcttta attaattgat
     7741 gaatgatccg caccaaagat tttatcttct tcttgttctt gcatttttgt cgccaattat
     7801 tcatgaaaac caagacgggt tcaggaagca aacgccgatg aaagcgtaaa gcaagtttgt
     7861 ggatgtcaaa cgtctgcttg atttccttta gtaacttttt atcaggtgtg gcacttaagt
     7921 agatgaatcg tccgtctggt ttaatcgctg tttgcacagc atgagctaat cctgcatccc
     7981 cgttataagg aaacgcatcg atctcatcga tcactattaa atcaaaggac cgataaaaac
     8041 ggtatagctg atgaatcgta cagataacaa aagaagtaaa ggtataagcc gttgctgtat
     8101 tcccatggag caacgtgatt ttttcttctg gaaaaacata acgatagcgt aaaaacaatt
     8161 cattacaaac atcgactcgc ggtgtacaaa tcgctactct tcctccagct tttaaagttt
     8221 ccactaaaat aggataaagc atctctgttt tccctgcacc tgttacagcc caaataagat
     8281 gatgtctttt ttctgaatgg caaagtattc tctttgagat ttccgtttgt ttttcagtaa
     8341 gctgtcctgt ccaaaaaaag acgacttctc gaggttttgc ttcaggctga tcaaacaaat
     8401 ataaatcttg ttgagtgtcg catcgcccta aatatagaca atgtgtacaa aaataaccgt
     8461 aatctgcaag ctgtccatca atgacggacg tcctcattcc acagcgatga caaagaatcc
     8521 agttgcccaa acgttccact gctgcgatct tctcataatt tggtaaattt acctcctcac
     8581 agtctattgc ttcaatatct ctcgtcagaa gccttctacc ccaaaaatga ttcatattct
     8641 tcgccctaaa aaaatatacg aaaaaacaag cgatctattt tttggatcgc ttgttcgata
     8701 gttatactgt tctatt
//
LOCUS       DAYYNG010000124         8606 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000124 DAYYNG010000000
VERSION     DAYYNG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.125"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..1099
                     /locus_tag="ACHBFA_002239"
     CDS             230..1099
                     /locus_tag="ACHBFA_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9395672.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            1119..1466
                     /locus_tag="ACHBFA_002240"
     CDS             1119..1466
                     /locus_tag="ACHBFA_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395673.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1479..2741
                     /locus_tag="ACHBFA_002241"
     CDS             1479..2741
                     /locus_tag="ACHBFA_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395674.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2821..4197
                     /locus_tag="ACHBFA_002242"
     CDS             2821..4197
                     /locus_tag="ACHBFA_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9395675.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            4194..4823
                     /locus_tag="ACHBFA_002243"
     CDS             4194..4823
                     /locus_tag="ACHBFA_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395676.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4842..5321
                     /locus_tag="ACHBFA_002244"
     CDS             4842..5321
                     /locus_tag="ACHBFA_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395677.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5880..6479
                     /locus_tag="ACHBFA_002245"
     CDS             5880..6479
                     /locus_tag="ACHBFA_002245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9395678.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIHFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6495..6971
                     /locus_tag="ACHBFA_002246"
     CDS             6495..6971
                     /locus_tag="ACHBFA_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395679.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            6988..7890
                     /locus_tag="ACHBFA_002247"
     CDS             6988..7890
                     /locus_tag="ACHBFA_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395680.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7963..8157
                     /locus_tag="ACHBFA_002248"
     CDS             7963..8157
                     /locus_tag="ACHBFA_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395681.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            8262..>8606
                     /locus_tag="ACHBFA_002249"
     CDS             8262..>8606
                     /locus_tag="ACHBFA_002249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395682.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNKE"
BASE COUNT         3292 a         1192 c         1701 g         2421 t
ORIGIN      
        1 tagagctgaa aagctctatt ttttttgata aaaaaactag ctattttatt aaaaataatt
       61 tggttcaaaa tttatttttt taacttgata attttttgcc atcctttttt aaattggcta
      121 tatatcaagg tttatagtgg gaataaataa aaatacattt gacaaaattt acaatatagt
      181 ttattatata tttatcttat tatgataaca caataaaagg aatggtggta tgaaaaagaa
      241 gaagtttagc cgatcgttgt taggaatttt ggttttagtt gcggttggtc ttagtggcaa
      301 gggttacttt cagaataaca gtactcagga acatgatttt cagatcaaaa gtagtgttca
      361 ggtaggtaat aaagcagaca gtgaatcaaa agaattttta gggatacgtt atgatccaaa
      421 caaatatcca aataatatcg taaacattaa tgatgggaaa tatacactga caaaagaaga
      481 ccatgaagca ttatcagaaa aaggcagtca aaccttttgg gtaaattatg cagatttaga
      541 tcaacttgga agagcaggag atgttgaagc gttgatcacc tatgatgctg taaattctca
      601 ctcatctaaa aatgtggaac gtccatcttt caaaagttct gtccatattg caggagaata
      661 caaggatgga gtatttgatc aagagagaga tacttgggtc ggcaaagatt caaacaatga
      721 aatcactcag ctagaaggct ataaaggtta tctctataac aaaagtcatt cattagcatg
      781 gtcgctagga ggagatatgg aggtacacaa tttaacactt ggaacgagag cgcaaaatgt
      841 tgggaaaaat gatcagaatg gagggatggc ttactcagaa acgattgtac gtgatgcaat
      901 ctataaaaat aagaatgaaa aagtattgta taaagttgat ccaatttata aagggaatga
      961 attagttcct cgtggttctc atgtcgaagc tttctcagtt aacgataatg gaaaaacggt
     1021 aaaccttaat gtttatgtat ttaacaatca aaaaggaatc aacatcaact atcaaaatgg
     1081 tacttgggta agagattaga aattgagaag gtgaagaatt ggaaacaata agcatcaatg
     1141 gattagataa atcagttgca aatcgaataa aaaaaggaaa tggctactat cttctgaaga
     1201 agggtaaata ttcagttgct agagagttag aaacaatgag tatcgtgtat gtagctaaga
     1261 atcattttga cgtagaacag gctatttttg atccagattc tgaaataggg aaatgtgttg
     1321 aattatcaga gaaaactgtt agtacaaaag gggcgatgat cattgagaga gaaatgttag
     1381 acgaaatcag agtaatcatt cctaaagata cgcagatgtg tgaggacagc ataagtgacg
     1441 atgaaccaaa atcagaaaga aaataaggag atattcagat gagcgaaaaa tccattgaat
     1501 taaaggaata taagcgaagc gatggagaaa aatattatcc tgtagccagt ttttctaaat
     1561 cagtatatct ggttggacgt gcattggtag ttggtgcaat tggcatatat attttgcatt
     1621 gggtaacaaa ggttgtacaa ctgcttgtag ataccttagt aagtattctt gtctcgttaa
     1681 atgatgttaa cggaacagat gatttaggat taaaagagat gtttaaagag ttactcaatg
     1741 cgttttttcc caattattta agttattcat ataatgtaca aagctttttt gcaaagctac
     1801 acttggtaga catctctatt ttagtattag aagcaggaac ggtattcttt gttggatatt
     1861 tggttacttt gatgttcagg catcactatg gagaacaaag accatttttt gatgatatgg
     1921 aaagtagaag gttgaaaaga gaagctctag aagctatgga tgtgaagtat gatccaaaca
     1981 agattatagg agatgaaacg aacaacaaaa aaagaaaacg aaacaaaacg caattagaat
     2041 ccgatgtgct atcaacgctt aaaaatatca aaatttctgt tcatacaaga cgtgatctaa
     2101 gtgtcaatga agatattcga gaatatatcg taaaaatccc gcaaccaaaa gggaagaaag
     2161 ttagagaaac agttttacga caaattcaag atttagggtc gatcctgaca agagcaacaa
     2221 gcggacgagc caaatttggt gaaaaagaag atgaagtttc aggcgattat ttcattttcc
     2281 gagcatcagt aaatattgaa agagaacgta aggaaagagt aagtaccaaa aacaaagaaa
     2341 cggaaagagc aacatcttca atcgcagttt caaattcaca attttctttt ccaatcaatc
     2401 tctttgccga taattccaaa aatatccaaa accaaaccga aaaagctaga aaatatgccg
     2461 aaactcaaaa gcaatcttta gaactttatc tatcttctgc tgacgtacct gcaagtctaa
     2521 cagatatgca cgttgggaat acatctattg aatatttgtt tactttatct cgtaccagaa
     2581 aaattcaact aagctcaatg aaagaagaaa ttgaaactta cttgcatata tcaggtgtaa
     2641 tcgtgcggat caacgcaggg cgtattcaaa ttactttacc attaggagaa aatgatcgta
     2701 ttccaattga tgtaccatca atgatcaata gcgtgtttta gaagacatca caaatagaaa
     2761 cagaaaatac aaactaccag ttcacaaaca gaaaagtacc gagaaaagtg aggaatagaa
     2821 atggctatga aattattaga gaaagtcaaa aagagagccg aaaaagctga atctattttg
     2881 ctagataaag aacctaatat aaagcttaat ggaaaattca gtgtcaatat gttgaacaca
     2941 aaggctaaag acccaacaga tgctattttt ggaattaagc ctgatggcac aattgctcat
     3001 ttttgcatca gcgaaatgcc acacatgttg gttgcaggaa cgacaggatc agggaaatca
     3061 gtattactga atgaaattct tgttacagta atgtgtcact caacgcctga tgaattaaaa
     3121 ataggaattg tagacccaaa aagggttgaa tttggaagat acaaaaaatt gccgttcatg
     3181 ttggctgatc caattacaga tatggatgaa gcttatgatt tttttgaata tcttgtgatc
     3241 ttgatgcatg aacgttacaa actgatggaa aaagcagggt tacaaaacat cacgctatat
     3301 aacgaatatg ccgaaaaaaa tggattagat agatttccct atgtgatctt attagttgat
     3361 gaatattcac agttggttgg aacacataaa gaagtagaag gtttgattgt tcaattagga
     3421 caaatggcaa gggcagcagg tattcatgtt atcttagcaa cacaatctcc tcgatcaaca
     3481 gttgtcactg gtattatcaa agccaatttt ccgtcacgtg tttgccttat ggtagcaagt
     3541 gatttagaaa gtaggattgt attagatgaa ggcggtggag aaagtctaag tccaaaggga
     3601 gatatgctta ttaagcttgt tgatggaagt atggttagag cgcagggagc ttacatcagt
     3661 aacaaagaaa ttgaaacaat cttcaatcac cttagaaata ctatgccaga accagaatat
     3721 gttgattacc gagcaatcgt agctgaaact cgtggtgagg gcggtgaaga cgaggatttt
     3781 ggtggaattg gtaaagctcc tgtaagaaaa tctgctaaaa gaaatttgaa cacaccttta
     3841 gcaaatccat catctaaaga gccatcaatt gaaaagaaga gtgcgtttgc tattaagctt
     3901 gaaaagaaca aagaagaaaa agtcgagcag ggagctaaaa cactagaaaa actgcaagca
     3961 gaaaaactag aaaaagtttt aagggcatta ggaaaagctg atcctgaacc gcaaacctca
     4021 aacaacacga ataataaaca gccgattgca tctaaaacca aacctaaagc taatgatcca
     4081 aaaggctcag aaaacgtagt gatcacaact gataaatctg aaaagaaaca aataaatgga
     4141 agtacttcac ctatcatgca aaaaatgtta aaagcaaaaa aacaggtgca taaatgatca
     4201 tcgtcagttc ttttttaaga tggcttactc catataaata tacagatgaa gtagttgtga
     4261 aacgagcgaa gaaaagagat aaaaaagcgg tactaactcc taaacagatg cttgatcatg
     4321 gcattttagg ggtgaaggtt ttagaagtga ataaaccgtt tgaatcaaat agtgcaagag
     4381 aaaagttgct tggagctgaa gccgatgtcg catattttta ccatgacaaa agcaagcatc
     4441 aaatagctat taaattcaat ccaaaatcgg catggcggta tcaagcaaga gatgacttga
     4501 aacgtaggaa acttagacca atgctctatc cagaaggaaa agaagcattt gatagaaccc
     4561 acatcgttcc cattggtttt catggtacag aatcagataa ccgcctatta gtaggttgga
     4621 acagcaatca aaatagagtt cagatcaata aatttgagaa gaaagtaagg aaattcaacg
     4681 atcgaaaaac gatcctatgg tttgcagata tttgtaaaca gccggatgga acagcaaagt
     4741 gggaagcggt gatatttgac gaaaatggta aaaaagtaat gtctgaaaca tggcatgata
     4801 aagctaaatt tgtttggcag tagcagtaga ggtgaaaaag attgctagaa ctttttgaag
     4861 caaatagaac aggctttaat caaaaattag aagtgggaga aactatagaa tataaagata
     4921 aaaactatat tattatcgga atctataaca cgagaattaa cagaataggt gatccacgta
     4981 taaccagtga cttggtttgt caatctgtta actattcacc tgatctgaca agcaggtata
     5041 agaaatacgt gttattagaa tatagacaca agattactga tgaaattggt gtaaacaaca
     5101 cacgaaacat atttaagatc ggaacagtga ttccttatag taacaacgaa gaaaaaatat
     5161 tttatcagat ttacggaata gaaaagtttg aatatgaaca tgtcgatctg ctagtgacgt
     5221 accaaatgcg tttaatagaa ccttggtcac aggcagaaat tgataaagca gtaaaattga
     5281 accgattatc aaaatttaac gtattaggaa atgcctttta aaaatcgcaa tacacaaaca
     5341 gaaaaatacc aaaaaattag caatgaacgt aggtaagaac atgacaataa ataataataa
     5401 ccctaaaaaa tataatgctc ttgtccaatg ctatatcgaa aacaaaatta caatgcaaga
     5461 agtcgctgat gaattaggca aatcaaagag cctagtatca cacgatttta agcacaatgt
     5521 ttttccgaac ccgattgagc tgaggaaaaa atttcaaaag agagtttatc aagaaatagg
     5581 taagtggatt gatgaaggga atgcaattga ccctttaatt gaaaaatatc cttttttaaa
     5641 aagcaaaaat aaaattcaaa ggttaattcg tcaaggcgat attcaagcta aagaacctgt
     5701 tttgacaacc aatgatttgg ctaaaataaa tcttcataaa caaattattg aagactttga
     5761 aaaaaatctt tcaacaactg aaattttaaa aaaatacaat attccgagag gaaagctaac
     5821 atcgactctt tttagagaat atggagaatc ggcagttact aaagcaaagg aagtagcgaa
     5881 tgagagaaaa aaacaaaaat agtagtcgat acctagtagc gactgtagtt tttgtttgtg
     5941 tattgatttt cctgtttgat tttttgcaga ttggaaaaag cataattgga aatacattga
     6001 gtgcaaagga aatggttgct ttaggtggtt tgtatgaagg acacttgttc ccgaatatat
     6061 tcatttctat cgtttctcat attaacttgt accatttaat ttctaattta ttgttcttag
     6121 tagttcttga agaagcactg aatgatcagt atgagtggtt aacattattg atcgttttaa
     6181 tcatatcagg atttctagga agcttagttc cttttctgct acacagcaac atcgtaagta
     6241 tggggcttag tggaggtatt tttggatgga ttgctttatt tgcgattgca tacatccatt
     6301 ttgatagcaa gaaacattta ttactaagtg tttttgtatt attgataatc ggcattttac
     6361 tggtatctac ttttgaatca ggcgagacaa acgtcttgtc acatgtaatc ggatttgtct
     6421 gtgggatgat cttgggattt attctaaaga acgtttcatt gatcaaacaa aagaattaaa
     6481 agggagtgct aaaaatgcca aaaaaaaaag aaaagaaaat tccagtgttt ttgtctgtct
     6541 cagaaatgtt atcacttcaa atactgttaa gaaaaaagat tgaaacaata gacgaagaag
     6601 ttagaaataa aagggctatg tacgaacaac gtgaagcgct aagagaacga atccactatt
     6661 ttgatgatat acacactaaa atctatgaag cacgtacaaa tcacgaagac gaaagcaaat
     6721 taggattagc tcaacaacga atatttgaat ggctgaatac gaatactgag caagaaccgt
     6781 taagagcaat tagtaatctt tttgaatttg gaacgaagca ttggtttaac gctgtagaaa
     6841 aggatgctga cttacaagaa gcgttcgata cactaagcga ggatagcaaa tccatgcgag
     6901 acttattgat cacttatcta aatgagcatg aatttgaaca gacgaaagtt aaaaagaaaa
     6961 ataaaaaata gaaagttgtg atgtatcatg ctgtttacct tattaggtta tttatgttta
     7021 gttcttgctt tttttggagc aatagctata tttgtcggtc agtttcaagc aaataatccg
     7081 ataatgcgta acataggcat tggaattgtt atcatatcct tgattgtatt tggtgccaca
     7141 aagatttttg ggagtaatga tcagcagttt ggtatttctg gagttgataa tagttatgct
     7201 ccagattcat tgggaagagt agtgatagat ggaacgtttg agaatgtaac gagctacaaa
     7261 aatgtttcat tgacagtgaa ggaagatggc aagaaagcat cagacgacgt tctcgctact
     7321 attaagccgt ctcaatcaca atttgaagtg ttttatacag ttaaagagaa tagccctgta
     7381 cacaagctga cattggtttt taacgttgat ggagaagaat actataaaga tgtaaaaata
     7441 aataaaacat caacgaaaaa gattatcgaa tcagcgaaaa aagaagtagc tacagaagaa
     7501 agtacaactg aatcaagttc tactgtcgaa gcggaactac cagataaaat tgattttgat
     7561 cctgaaaaag tcaaatcgtt tgaatctgtt tctggttcta tggcagatcg gtttaacact
     7621 gaagttttca aagctatcaa aactgttaaa ttggatactg aaaagcaaat gattaatgtt
     7681 accttattca atgcagttga atctatggat caggataaaa aagttacggt tgcaagagaa
     7741 ctagggaaaa gagtaattgg gttgtatcga ggtacggcaa aaccagattc agacggttca
     7801 gatttgccag ttcacgtagt ttatgaagac ggtaaagttt ttggagactc tacagcaaat
     7861 gaagcactaa cgattaaatt agctaaatag ccggaaaatt ataataaaca agtggtgtca
     7921 caccaccatc gcataggtgt gacaccttgg agatgattac tgatggtagt aaataaagaa
     7981 aaagtaagaa agtatgttac tttaaaaata gcttctgttg aatatttaga aaagcgagct
     8041 aaagaagaag aaaaaacaag aggtaagaag gtaacagttt cagagttgtt agaagaatta
     8101 attgagagtg accaaacaat aagacgtgca tttaccaata ggtctgttaa aagttagggt
     8161 gatactatct ttaatttcaa ccaaatttct aatatatgat gaatagtttc cttgtacaaa
     8221 atatctaatt aagagataaa agttaggagg attcattcaa aatgattatc caaaagtttg
     8281 actggaaaat gaaattagga attagtcttg taatattggg atttattatt tttttgggga
     8341 taaaagcaca aaaaaatcaa tctacttttg aaactatgtc ttctcaaatt attgaggaat
     8401 gttccaaaat ttattattgt gagaacaaaa ctgaagaatc taatggcttt tattatatcg
     8461 atcaaccaca tgatgttgtt ataaaaatgg agtcacatga agtaaacgaa tggattcaaa
     8521 gaagtactaa tgtatcaaaa aagttagaaa gatattctca aacgttttta agtaaagtca
     8581 ttttttcaaa ttgcaaaaat aaagaa
//
LOCUS       DAYYNG010000125         8555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000125 DAYYNG010000000
VERSION     DAYYNG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.126"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACHBFA_002250"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACHBFA_002250"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM9395683.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACHBFA_002251"
     CDS             complement(1098..2201)
                     /locus_tag="ACHBFA_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9395684.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACHBFA_002252"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACHBFA_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM9395685.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="ACHBFA_002253"
     CDS             3528..5462
                     /locus_tag="ACHBFA_002253"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9395686.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="ACHBFA_002254"
     CDS             5919..6335
                     /locus_tag="ACHBFA_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9395687.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="ACHBFA_002255"
     CDS             6349..6915
                     /locus_tag="ACHBFA_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM9395688.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="ACHBFA_002256"
     CDS             6938..7924
                     /locus_tag="ACHBFA_002256"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9395689.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="ACHBFA_002257"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="ACHBFA_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9395690.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2517 a         1651 c         1736 g         2651 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttac cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DAYYNG010000126         8484 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000126 DAYYNG010000000
VERSION     DAYYNG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.127"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHBFA_002258"
     CDS             complement(<1..149)
                     /locus_tag="ACHBFA_002258"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9395691.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHBFA_002259"
     CDS             complement(593..1714)
                     /locus_tag="ACHBFA_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM9395692.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHBFA_002260"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHBFA_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM9395693.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHBFA_002261"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHBFA_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM9395694.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHBFA_002262"
     CDS             complement(4304..5215)
                     /locus_tag="ACHBFA_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9395695.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHBFA_002263"
     CDS             complement(5232..6239)
                     /locus_tag="ACHBFA_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9395696.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8355)
                     /locus_tag="ACHBFA_002264"
                     /pseudo
     CDS             complement(6236..8355)
                     /locus_tag="ACHBFA_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(8360..8470)
                     /locus_tag="ACHBFA_002265"
     CDS             complement(8360..8470)
                     /locus_tag="ACHBFA_002265"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395697.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1723 c         1485 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt cgttcaatac
     6541 tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc gcaactcccc
     6601 tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt ttttcgtctt
     6661 ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta cttgattttt
     6721 tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc caacttttct
     6781 accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt gttttttcag
     6841 atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg acactctatt
     6901 gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac caactgctgc
     6961 accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca attttcgatt
     7021 catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat cattactttg
     7081 aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa aggtcacaat
     7141 ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa acatcgtgga
     7201 gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa tcgtattgaa
     7261 caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg acaataagaa
     7321 actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca acataatccc
     7381 tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa agaccacggt
     7441 tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat cttcaatctc
     7501 tgctttgact gcttcttccc gatccttacc cttattttca tccggactca cggataaaat
     7561 cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt gtaatagtaa
     7621 ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca cactatcttt
     7681 tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag ccgcttcact
     7741 aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg caatgaaaga
     7801 acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt tggtagtttc
     7861 tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca tcaataatag
     7921 gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg ctaaagtttg
     7981 gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt aagcttcttg
     8041 aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa aattggtaat
     8101 ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc aaggtaacca
     8161 atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat atttggaata
     8221 aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta ccgtaccgac
     8281 taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca caatccgaaa
     8341 aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga gtgtcaaaag
     8401 catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc ccatacaagt
     8461 cttgcatcaa gcattgaccg tttt
//
LOCUS       DAYYNG010000127         8440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000127 DAYYNG010000000
VERSION     DAYYNG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.128"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..2731
                     /gene="clpB"
                     /locus_tag="ACHBFA_002266"
     CDS             122..2731
                     /gene="clpB"
                     /locus_tag="ACHBFA_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM9395698.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2882..3568)
                     /locus_tag="ACHBFA_002267"
     CDS             complement(2882..3568)
                     /locus_tag="ACHBFA_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395699.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3546..4040)
                     /locus_tag="ACHBFA_002268"
     CDS             complement(3546..4040)
                     /locus_tag="ACHBFA_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9395700.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4060..5280)
                     /locus_tag="ACHBFA_002269"
     CDS             complement(4060..5280)
                     /locus_tag="ACHBFA_002269"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9395701.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5427..7262
                     /gene="lepA"
                     /locus_tag="ACHBFA_002270"
     CDS             5427..7262
                     /gene="lepA"
                     /locus_tag="ACHBFA_002270"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9395702.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7356..8438)
                     /locus_tag="ACHBFA_002271"
     CDS             complement(7356..8438)
                     /locus_tag="ACHBFA_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9395703.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2717 a         1614 c         1698 g         2411 t
ORIGIN      
        1 gagaaaaaaa gtcaaaaaac tatgtgtata ttttgaccaa aattgaccaa agcgatataa
       61 tagtttttgt aaagatcaaa gaaataagtg atcttacctt taattaggag ggtatacctg
      121 tatgaatatt gagaaaatga ctacgacgct tcaagaagcg attgctgaag ctcaacagat
      181 tgcggtcact cgaaaacatc aagatatcga cattgcccat gtatggaaaa tctttttaca
      241 acctaatcat tttggtcgaa atttctatac ggatgccgga ttggatgtcg aatcttttga
      301 acatgaaatt gatcgtctac tggatgagta tccagttgtt tctggtggaa acgtccaata
      361 tggacaaaac ctcagccaga atttctttcg attattgaat gaagcagacc aaatacgtga
      421 aagcttcggc gacgagtttc tttcaacgga agtcgttatt ttagcgttga tgaaattaaa
      481 aaattatccg ttaactgttt atttgaataa aaacggatta tctgaaaaag aattgcgtaa
      541 aaacatcgaa gagatgagag gaggagaaag agtgacctca caaaaccaag aagaacaata
      601 caaagcatta gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga
      661 tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac
      721 aaaaaataat cctgttctga taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg
      781 gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt
      841 ttcattagat atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag
      901 actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga
      961 tgaaatccac aatatcgtag gggcaggaaa aacagaagga agtatggatg ccggaaatct
     1021 gttgaaacca atgcttgctc gcggtgaatt acatttgatc ggtgccacaa ctttagatga
     1081 gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt cgctttcaaa aggtattagt
     1141 caaagaacca acagtggaag atacgatcag tattctgcgt ggattaaaag aacgttttga
     1201 gatccaccat ggtgttaaca tccatgacaa cgcattagta gcagcagcta ctttatctga
     1261 ccgttatatt actgatcggt tcttaccgga caaagcaatc gatttgatcg atgaagccag
     1321 tgcaacgatt cgggtagaaa tgaactctat gccaacggaa ttagatcaag ttactagacg
     1381 tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa
     1441 aaaacgcttg aaaaatttgc aagaagaatt agctgaactt cgtgaagaag caaatgcgat
     1501 gaagatgcag tgggaaacag aaaaagaaga agtcaattct gtttcagcaa aacgtgctga
     1561 aatcgataaa gcaaaacacg aattagaaga tgcagaaaat aactatgatt tagaacgcgc
     1621 tgctgtattg cgtcatggta cgatacctca attagaaaaa gaattgaagg aattagaagc
     1681 taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa atgagattgc
     1741 tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga
     1801 aaaactaatt aaattgaatg aaacacttca taaacgagtg attggtcaag atgaagcagt
     1861 tgatgcggtc agtgacgcgg taattcgttc acgggctgga ttgcaagacc caaatcgtcc
     1921 tctaggttct ttccttttcc taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc
     1981 gttagctgag aacttgtttg actcagaaga tcatatggta cggatcgata tgagtgaata
     2041 tatggaaaaa catgctgtgt ctcgtctagt cggagctcct ccaggctatg taggttacga
     2101 agaaggtggt caattaacgg aagctgtcag acgaaatcct tatacaatcg tcttgttgga
     2161 tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc tagatgacgg
     2221 acgcttgact gattcaaaag ggcgagtagt agatttcaaa aatacggttc tgatcatgac
     2281 cagcaatatc ggttcacagt tactgctgga aggcgtgact gctgatggaa cgattccaga
     2341 agcagtggct gaacaagtca acacgctatt acgtggaaac ttcaaaccag aattcttgaa
     2401 ccgaatcgac gacacgattt tatttacacc attgagccta gacaacgtga aaggaatcgt
     2461 tgacaaaatg gtggcgcaat tggcacaacg tctggaacat caagaaatcc tgctgacaat
     2521 cagtgatgaa gccaaaacgt ggattgctga aaatgcttat gaaccagcat atggtgcaag
     2581 accattgaaa cgatttatca ctaaggaagt agaaacacca ctagctaaag aaatcgttgc
     2641 aggacatgtt atgcctaaat caaaagtaac gattacttta cttgatggac aacttcattt
     2701 caaaacagaa gagctagaag aaatcgtcta atcttccatt gagactataa aagaaaaaag
     2761 gggtctgaca caactagtgt cagacctcct tttttcaaat aaacagtgtt ccagaagtag
     2821 tttcttcgaa aaataagccg aaatttcttg aagctgcccg cttctgtcac aacctctttt
     2881 attaggtatt tttcactttt tctaatttat aactttcaaa gtaacgctca tcttttttac
     2941 tttgagacca tgcaaacaat cccattaagg cagcacctat cgtatttgtc agcaagtcgc
     3001 ccatcgtatc catcaatgct gctcgtccga caaacagtgt gccatcagaa gcagcaaaac
     3061 gttgaagatt catgcctaag aactggtcgc ataagaattc ccagaactcc caaaacacgc
     3121 cgcaaagtcc agcaaacgca aagccgaata agaggaacaa ccaaggactg gtttttgata
     3181 tctcagcatc tttcatcaag taaccaatca atccatagcc aagagctgtc aggaccattg
     3241 gactcaccgc atgtaagatt ttgtcccaaa aagagatgat actgatcaaa tgcatccctg
     3301 tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa caaaacgatt gctgctggta
     3361 ttttaagttt gaaaactttc aaaatgactt gtggcatgaa aatcaatgca atccctaata
     3421 aagattccac gataaatgtg attccttgag aagtcgaata tttttgtgtg ataaattcat
     3481 aaaaattata gcagacaacg aatacaccaa aagcaagtaa agctatttgc acctttttga
     3541 tttctttaat cactgaaagt ccctccatct aaaaatcttt ttacttctga aagcaactca
     3601 atttctgaag aaaccacttt tccgttcaat ttgatcaaac ctattgtata taagttcagg
     3661 tagtgaaatt gattttcagc tgtttcttgt agtgcagcga tttttttcgg gtgggttccg
     3721 ccttgctgtc gagtatcagt atataagccg atcactggga tgcctttcgc ataagccaca
     3781 ccgatttccg atgctacccc ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa
     3841 tcactagcca acactttttc tgtgtcagca agagcaatca ttcgactgtc tgcataagca
     3901 gttttgtcat tgattgcttc attttcttga ggaagataga tcgaaagctc agaagagagc
     3961 tctctgattt ctttaactaa ctgttggtta tataacaaat cacttttagc aaacattggt
     4021 gcggcaaaat aaattttcat ctaaacatca tcctttttat caaaaataat cgctaaagta
     4081 agcttccatg tttggaatga tacgctctag tgacttcagt gtttcttcat ctgtttctaa
     4141 ccgttcgatt cttgccaaat aagaagcacg gcgataattc atcatcaagc aagtcaatgt
     4201 ttggatatcc agtcgaacgg attttcctat aggttcattt aagacacgga cttgtccctg
     4261 ttcatcccat gttaaagcga agacaccatt attccaagga gcgacaggat cttcaatgat
     4321 gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc
     4381 aacgatccga gccataaaat atggttcgat ctgctctttg atctgactat cttcaatcaa
     4441 aaatgctaaa ggctcatttt tataaatatc tccatgtacc cagtagatca tggaaaaatg
     4501 tgcggaaata aagttccata agccatttcg agcttcttga tcaagataga acatttcttt
     4561 tacgtgaaat atttcttcag ctacccagta gaacagaaca cctcttggtt catggttgct
     4621 atcgtagtag acagcggctg tgcgctcatc ttcattttcg aaacgccaat actcttccca
     4681 atgaaaatca ctgcggaata aagcaccatg gttttgttgg gcaaatttgt cataaacgga
     4741 aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata ccacttactg ggatttgttt
     4801 aggtagctgt gtatctctga ttttaaagga aagtttatca gacatgattt cccatccttt
     4861 acgccgatag taggggatac tgtaaggata caaataagag atccattgct tattcttgcg
     4921 catattatcc aatgcaacga taattagatc ttgcatcaat ccatggttag catattctgg
     4981 ataagttcca actccagtca caccacccat tttataacgt gcaccgtgaa tattgacttc
     5041 acaaggatag atagcgattt gagagatcag tttattttcg tggaaccatc cgaacacttt
     5101 cgatacttcc aaaatcggtt ttttggaacg gataaaagct cgtttattct caaacccgct
     5161 ttcctcgatg tctgcttcag tcacttggaa cacataggaa agcaattcat tgaattgatc
     5221 gatatgttct tgagtgaccg gttttaattc aagattttct ctaaaatctt tttgatccat
     5281 tttttcatct ccatttatta gcttgataac agtatagcaa aattctacaa aaaaaacttc
     5341 tatagctgac taattaaaaa ctctttaaat tactgctttt cgttggtata atggaaagtg
     5401 ctgattatat gaaaagagga gacgctatgg atataaagaa aatgaaagaa cgccaagagc
     5461 agattcgtaa tttttcgatc atcgctcata tcgaccatgg gaaatctaca ttggccgatc
     5521 gtattttaga aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag ctgttagact
     5581 cgatggattt ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta gaactcaatt
     5641 acacagcgaa aaatggagaa acatatatct ttcacttgat tgacacccca gggcacgtcg
     5701 acttcaccta tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg
     5761 atgcagcaca aggaatcgaa gcacaaacat tggccaatgt ttatttagcg ttagacaatg
     5821 atttggagat cctgccagtc atcaataaaa tcgacctacc cgcagcagat cccgaacgtg
     5881 tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag tgaagctgta ttagccagtg
     5941 ccaaatcagg gattgggatt gaagatattc tcgagcagat cgtggaatat gttcctgcac
     6001 caagtggaga cttagatgca ccgctaaaag ctttgatctt tgactctatt tatgatagtt
     6061 accgcggtgt tgttctaaat gtccggatca cggacggtat ggtcaaaccc ggagacaaaa
     6121 tcaaactgat gagtaatggc aaaacatttg atgttacaga ggtcggggtc ttctcgccaa
     6181 aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca
     6241 aaactgtcca agatacacga gtaggggata cagtaacgtt agcagacaat cctgccgctg
     6301 aagctttacc aggttaccga aaaatgaatc cgatggttta ttgtggtctt tatccaattg
     6361 atacttctcg ttacaacgat ttgcgggaag ctttagaaaa actgcaattg aatgatgctg
     6421 ctttacaatt cgaaccagaa acctctcaag cattaggttt tgggttccgc tgtggattcc
     6481 tcggacttct tcatatggat gtcgtccaag aacgcttgga acgggaattc aatctagaat
     6541 tgatcacgac tgcaccatcc gttatttacc atgttaataa aacggatggt acgactgcta
     6601 cagtagataa cccggcagat tttcctgaac ctgtaacgat tcaggatgta gaagaaccat
     6661 tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg tgcagtaatg gaactttctc
     6721 aacgaaaacg gggagaattc attacgatgg attatttaga tgattaccga gtaaatgtcg
     6781 tctataatat tccgctttct gaaattgtct ttgacttttt cgacaaattg aaatcaagca
     6841 cgaaaggcta tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc aagttagtga
     6901 agatggacat cttgctgaat ggagaaaaag tcgatgcatt aagctttatc gttcaccgag
     6961 actttgccta tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc
     7021 aacaatttga ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc cgttcagaca
     7081 tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt tctcgtaaac
     7141 gtaaactctt agagaaacaa aaagaaggga agaagcggat gaaacaaatc ggctccgtcg
     7201 aagttccgca agaagccttc atggccgttc tgaaaatgga cgaggacgaa ccgaagaaat
     7261 agcaagggtt tgtttgaaaa cagtgtaaaa aataaaccat gtttacatgg atttgccacc
     7321 ggtttgccac cggtggtttt tttcggtggc aaaaatcaag aattctttct aagattttcc
     7381 atgatatcga ccgtttcatt tctcatctta tctgttacat gcgagtaagt atccatcgta
     7441 attgaaattc tgctgtggcc caatcgttca gagatttctt tcatttttgc gccattttcg
     7501 agaagaagtg tagcatgagt atgtctgaga gaatggaagt taaaagagag ggagagtgca
     7561 ttcgatatcc tcctagtatt ccatttcact acacttggcg taactagctc accgtcttcc
     7621 ttcgtacata ctgcatttga atcaatgtag agctttccat acttcattcg attttctaat
     7681 tgttgtttct tatgtttttt cagaattgct agcaaggttt gtccaataaa aatcgttcga
     7741 ttggaactgc ttgtctttgg cgtaccatat acccatgcgc cgtcattctt taccatttgt
     7801 ttctctacag taattgttcc atttgaaaaa tcgacatcat cccacgtcag accacaaact
     7861 tcgccaacgc gcattcccgt ataaaatcca atattcaaag gaatatagaa aggatgtcct
     7921 tcaggagtaa tttctagcat atgatcaaag tcctcaagag aaatgatttt tagatctttt
     7981 ttagtcgttg gtcgtgcttc atattttggt atttttacat acagcatagg attttgcttg
     8041 attaatcccc aaggataaac cgccatattc agcgcattct taaggacaga gtgagtaata
     8101 gtcattgttt tcttcgagta acccttttta aattcagcat tgatgaaatt ttgtaaaaga
     8161 gcaggggaga gatccgtaag ttttttcttt cctaaataac cgtttatatg atttttgatg
     8221 gtaaatcggt agttttcata ggtattgtat tttagattta gtttaacgta ttcctccatc
     8281 caaaaatcaa ggtattgttt tactcgagta tccgtaccta aaaagtattg tcctgtttcg
     8341 tcaatatctg ataaaacttt tcgtaaagca gcttcggcct ctggtcgggt gtctccgcca
     8401 actttctcca ctttttttct tgagccatca tcattgatat
//
LOCUS       DAYYNG010000128         8385 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000128 DAYYNG010000000
VERSION     DAYYNG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.129"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1055
                     /locus_tag="ACHBFA_002272"
     CDS             <1..1055
                     /locus_tag="ACHBFA_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9395704.1"
                     /translation="KVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLL
                     PQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFL
                     GKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSV
                     SPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYY
                     SPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANI
                     VNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRR
                     LNSRIDKVRYMFNKIT"
     gene            1153..1341
                     /locus_tag="ACHBFA_002273"
     CDS             1153..1341
                     /locus_tag="ACHBFA_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9395705.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1399..1752)
                     /locus_tag="ACHBFA_002274"
     CDS             complement(1399..1752)
                     /locus_tag="ACHBFA_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395706.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2283..2705
                     /locus_tag="ACHBFA_002275"
     CDS             2283..2705
                     /locus_tag="ACHBFA_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9395707.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2702..2932
                     /locus_tag="ACHBFA_002276"
     CDS             2702..2932
                     /locus_tag="ACHBFA_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395708.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3430..3630
                     /locus_tag="ACHBFA_002277"
     CDS             3430..3630
                     /locus_tag="ACHBFA_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM9395709.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3657..4850
                     /locus_tag="ACHBFA_002278"
     CDS             3657..4850
                     /locus_tag="ACHBFA_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9395710.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4918..6483)
                     /gene="guaA"
                     /locus_tag="ACHBFA_002279"
     CDS             complement(4918..6483)
                     /gene="guaA"
                     /locus_tag="ACHBFA_002279"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9395711.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6542..7465)
                     /gene="coaA"
                     /locus_tag="ACHBFA_002280"
     CDS             complement(6542..7465)
                     /gene="coaA"
                     /locus_tag="ACHBFA_002280"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9395712.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7661..8266
                     /locus_tag="ACHBFA_002281"
     CDS             7661..8266
                     /locus_tag="ACHBFA_002281"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9395713.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
BASE COUNT         2730 a         1516 c         1591 g         2548 t
ORIGIN      
        1 taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt
       61 attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag
      121 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
      181 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
      241 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
      301 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
      361 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
      421 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
      481 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
      541 taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat
      601 tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta tactatagcc
      661 ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag caagccttta
      721 gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat gcaccacagg
      781 aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc gtaaatactc
      841 aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt attcaagatt
      901 atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca gagctaaaag
      961 gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat agtcggatag
     1021 ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt tatataaata
     1081 tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta aagtggaaag
     1141 gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca
     1201 agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt
     1261 aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac
     1321 gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt
     1381 ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac cttttgctag
     1441 tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa
     1501 ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg
     1561 gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat
     1621 atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc
     1681 ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc
     1741 ttttttacgc atactaaatc acctctatac attttactgt tcctatataa ccataaacag
     1801 gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa
     1861 tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat
     1921 aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc
     1981 gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt
     2041 atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa
     2101 aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata
     2161 aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta
     2221 gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa
     2281 gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac
     2341 aagttattga agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag
     2401 aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt
     2461 acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt
     2521 tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt
     2581 actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg
     2641 tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag
     2701 aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga
     2761 cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt
     2821 acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg
     2881 cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt
     2941 tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca
     3001 tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac
     3061 aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc
     3121 atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga
     3181 ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt
     3241 tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg
     3301 tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat
     3361 aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa
     3421 ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct
     3481 ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac
     3541 tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt
     3601 cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg
     3661 ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt
     3721 atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag
     3781 caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg
     3841 ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg
     3901 taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg
     3961 ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat
     4021 caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg
     4081 atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga
     4141 aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc
     4201 taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta
     4261 tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact
     4321 caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg
     4381 ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt
     4441 atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct
     4501 ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc
     4561 tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa
     4621 tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga
     4681 cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat
     4741 taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc
     4801 aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta
     4861 aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct cctacaacta
     4921 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
     4981 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
     5041 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
     5101 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
     5161 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
     5221 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
     5281 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
     5341 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
     5401 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
     5461 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
     5521 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
     5581 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat cgactttgat
     5641 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
     5701 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
     5761 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
     5821 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
     5881 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
     5941 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
     6001 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
     6061 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
     6121 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
     6181 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
     6241 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
     6301 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
     6361 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
     6421 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
     6481 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
     6541 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
     6601 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
     6661 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
     6721 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
     6781 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
     6841 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
     6901 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
     6961 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
     7021 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
     7081 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
     7141 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
     7201 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
     7261 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
     7321 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
     7381 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
     7441 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
     7501 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
     7561 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
     7621 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
     7681 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
     7741 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
     7801 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
     7861 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
     7921 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
     7981 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
     8041 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
     8101 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
     8161 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
     8221 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
     8281 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
     8341 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaa
//
LOCUS       DAYYNG010000129         8319 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000129 DAYYNG010000000
VERSION     DAYYNG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.130"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1456)
                     /locus_tag="ACHBFA_002282"
     CDS             complement(80..1456)
                     /locus_tag="ACHBFA_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9395714.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1584..2672)
                     /locus_tag="ACHBFA_002283"
     CDS             complement(1584..2672)
                     /locus_tag="ACHBFA_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9395715.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2844..3124
                     /locus_tag="ACHBFA_002284"
                     /pseudo
     CDS             2844..3124
                     /locus_tag="ACHBFA_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3202..3735)
                     /locus_tag="ACHBFA_002285"
     CDS             complement(3202..3735)
                     /locus_tag="ACHBFA_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9395716.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3829..5007)
                     /gene="mutY"
                     /locus_tag="ACHBFA_002286"
     CDS             complement(3829..5007)
                     /gene="mutY"
                     /locus_tag="ACHBFA_002286"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9395717.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(5000..5797)
                     /gene="recX"
                     /locus_tag="ACHBFA_002287"
     CDS             complement(5000..5797)
                     /gene="recX"
                     /locus_tag="ACHBFA_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9395718.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            5904..7286
                     /gene="rlmD"
                     /locus_tag="ACHBFA_002288"
     CDS             5904..7286
                     /gene="rlmD"
                     /locus_tag="ACHBFA_002288"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9395719.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7460..8038)
                     /locus_tag="ACHBFA_002289"
     CDS             complement(7460..8038)
                     /locus_tag="ACHBFA_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM9395720.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         2503 a         1679 c         1434 g         2703 t
ORIGIN      
        1 tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag caacacgtct
       61 ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct tttctgaagt
      121 atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa atgacacgga
      181 ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta tcgacatcaa
      241 acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa taaccagcca
      301 tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca
      361 gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga ttctcaactt
      421 catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct agtgttgcta
      481 acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc gtctttttca
      541 tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc tcctgcatga
      601 ttttcttgat atctaaatta tcaaaaccga atttatgggc agcttttagt aaattctttg
      661 tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt cttgaatagt
      721 tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca atcataaatg
      781 catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa aaaacccctt
      841 ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata taacgcattt
      901 catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata cgcgccaata
      961 aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca atcaagcgta
     1021 tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata cgtttagggt
     1081 acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg atatttgcaa
     1141 tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat aaagtattag
     1201 ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt gtcgatagga
     1261 aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct ccttcatcag
     1321 ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa gctgctaaaa
     1381 aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc gactgactct
     1441 cagggtcagc attcataatc gcattctctc cttataaaat taatttttag tttacgatag
     1501 aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt tctttgcaat
     1561 aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt gttttcattt
     1621 tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca gattgcgtaa
     1681 aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac tagaagaacg
     1741 ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg ataaatgata
     1801 ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc ttttaccgga
     1861 tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc taaatatgga
     1921 attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac accaattacc
     1981 atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg tccgctaatg
     2041 taattagcca atgttttatt cagttgcccc aataactcca caatttggtt tcttcttttt
     2101 tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa catatagaac
     2161 aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc aacgatcgaa
     2221 cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta agaaatgtct
     2281 aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc ggctacctct
     2341 tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt
     2401 tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc aactaaaagg
     2461 agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa cagcttgaca
     2521 ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa taacgtggaa
     2581 aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat gatcaatgca
     2641 gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag tttttcaaac
     2701 aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa attcttttat
     2761 tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc cagtatacta
     2821 tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca atgagcaata
     2881 tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag ggtatgcctt
     2941 tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg tactcagata
     3001 aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa atgaaattac
     3061 agcggatggc gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt
     3121 ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg
     3181 gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat
     3241 atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt
     3301 cacattttct tttaggatga agtcaatatc gttcggataa ttcatcattt tgctgtgcat
     3361 ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa
     3421 gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca
     3481 ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg
     3541 aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa
     3601 tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg
     3661 ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc
     3721 ttttggaact cccatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt
     3781 tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt
     3841 cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac
     3901 gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg
     3961 ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa
     4021 aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa
     4081 tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt
     4141 ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt
     4201 ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg
     4261 attttgaaga gccgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac
     4321 aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt
     4381 ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt
     4441 gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct
     4501 gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac
     4561 gttgccgtca accgcgggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt
     4621 atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc
     4681 aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc
     4741 aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt
     4801 aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg
     4861 aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt
     4921 tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc
     4981 tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga
     5041 tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt
     5101 taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat
     5161 attctatttc ttgatctgct tcttgcggaa gatcagccat cgcttgttgg atcacttctt
     5221 gagaaaaacc tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt
     5281 ttccgcgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt
     5341 caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca
     5401 tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac
     5461 tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat
     5521 ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc
     5581 gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct
     5641 tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta
     5701 ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag
     5761 ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat
     5821 tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag
     5881 aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc
     5941 ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga
     6001 tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa
     6061 ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag
     6121 tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc
     6181 aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag
     6241 caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata
     6301 gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt
     6361 tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc
     6421 gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct
     6481 atgatgaacg gacaaacagt ggaatcttcc gcacgatcat ggttagaatc ggtgttaaaa
     6541 ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa
     6601 tgatctgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg
     6661 cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca
     6721 ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca
     6781 atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta
     6841 atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac
     6901 aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa
     6961 atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc
     7021 tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta
     7081 cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt
     7141 atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca
     7201 agattgaata tttacagtct gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg
     7261 tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc
     7321 aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac cgtacttgtg
     7381 accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg
     7441 tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg
     7501 tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa
     7561 gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga
     7621 tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt
     7681 agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc
     7741 ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc
     7801 cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac
     7861 tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc
     7921 ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa
     7981 aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt
     8041 agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt
     8101 atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa
     8161 cagaagtaga cgagaaaagt acaaatagaa aacatgagaa ccaaatcata tgatcttttt
     8221 caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat catcaatttt
     8281 tttcttactt cacaaactaa atacaggtct tgctgtgag
//
LOCUS       DAYYNG010000130         8292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000130 DAYYNG010000000
VERSION     DAYYNG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.131"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1938
                     /gene="nrdE"
                     /locus_tag="ACHBFA_002290"
     CDS             <1..1938
                     /gene="nrdE"
                     /locus_tag="ACHBFA_002290"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9395721.1"
                     /translation="SPSFIEKLYQFLDEQHFSFKSFMAAYKFYSQYALKTNDGSAYLE
                     TYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLNAGRKRRGELVSCFLTQ
                     VTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFED
                     SFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIRVKTLSLGVIVPDKFYE
                     LTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNIRKQKIKARDLENEISK
                     LQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPSVINGKQEYEVLGTDIS
                     CNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPPIQNGNKLNHTIGLGAM
                     GLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIARERKQVFHNFEKSAYAD
                     GTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVMKDGLYHQNRLAVAPNG
                     SISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETIPYYTSAYDMDMRKVID
                     VYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLNILRHYAFHKGIKSIYY
                     VRTFTDDAEEIGSNQCESCVI"
     gene            1988..2953
                     /gene="nrdF"
                     /locus_tag="ACHBFA_002291"
     CDS             1988..2953
                     /gene="nrdF"
                     /locus_tag="ACHBFA_002291"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9395722.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3042..4181)
                     /locus_tag="ACHBFA_002292"
     CDS             complement(3042..4181)
                     /locus_tag="ACHBFA_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9395723.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(4490..5203)
                     /locus_tag="ACHBFA_002293"
     CDS             complement(4490..5203)
                     /locus_tag="ACHBFA_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM9395724.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(5457..6158)
                     /gene="nagB"
                     /locus_tag="ACHBFA_002294"
     CDS             complement(5457..6158)
                     /gene="nagB"
                     /locus_tag="ACHBFA_002294"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9395725.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            6392..7924
                     /locus_tag="ACHBFA_002295"
     CDS             6392..7924
                     /locus_tag="ACHBFA_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9395726.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8115..>8292)
                     /locus_tag="ACHBFA_002296"
     CDS             complement(8115..>8292)
                     /locus_tag="ACHBFA_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9395727.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         2749 a         1639 c         1458 g         2446 t
ORIGIN      
        1 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
       61 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
      121 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
      181 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
      241 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
      301 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
      361 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
      421 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
      481 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
      541 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
      601 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
      661 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
      721 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
      781 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
      841 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
      901 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
      961 aacggcaaac aggaatatga agtattagga acagatatct catgtaactt aggctcgaca
     1021 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
     1081 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
     1141 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
     1201 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
     1261 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
     1321 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
     1381 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
     1441 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
     1501 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
     1561 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
     1621 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
     1681 atgcgtaaag taatcgatgt ttatgcaacg gcacaacaac acgtcgatca aggcatgagt
     1741 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
     1801 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
     1861 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
     1921 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
     1981 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
     2041 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
     2101 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
     2161 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
     2221 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
     2281 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
     2341 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
     2401 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
     2461 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
     2521 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
     2581 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
     2641 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
     2701 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
     2761 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
     2821 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
     2881 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
     2941 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
     3001 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
     3061 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
     3121 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
     3181 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
     3241 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
     3301 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
     3361 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
     3421 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
     3481 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
     3541 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
     3601 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
     3661 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
     3721 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
     3781 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
     3841 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
     3901 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
     3961 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
     4021 ataaaaccag caacaagcaa tggtaaaaaa attgccgaaa gcattttcaa tattggacta
     4081 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
     4141 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
     4201 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
     4261 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
     4321 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
     4381 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
     4441 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
     4501 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
     4561 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
     4621 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
     4681 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
     4741 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
     4801 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
     4861 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
     4921 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
     4981 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
     5041 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
     5101 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
     5161 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
     5221 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
     5281 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
     5341 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
     5401 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
     5461 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
     5521 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
     5581 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
     5641 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
     5701 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
     5761 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
     5821 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
     5881 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
     5941 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
     6001 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
     6061 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
     6121 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
     6181 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
     6241 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
     6301 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
     6361 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
     6421 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
     6481 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
     6541 tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc acaaaggtta
     6601 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
     6661 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
     6721 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
     6781 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
     6841 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
     6901 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
     6961 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
     7021 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
     7081 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
     7141 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
     7201 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
     7261 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
     7321 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
     7381 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
     7441 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
     7501 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
     7561 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
     7621 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
     7681 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
     7741 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
     7801 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
     7861 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
     7921 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
     7981 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
     8041 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
     8101 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
     8161 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
     8221 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
     8281 aacataaata ca
//
LOCUS       DAYYNG010000131         7979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000131 DAYYNG010000000
VERSION     DAYYNG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.132"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHBFA_002297"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACHBFA_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9395728.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="ACHBFA_002298"
     CDS             1228..2331
                     /locus_tag="ACHBFA_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9395729.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2328..2534)
                     /locus_tag="ACHBFA_002299"
     CDS             complement(2328..2534)
                     /locus_tag="ACHBFA_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395730.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="ACHBFA_002300"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="ACHBFA_002300"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9395731.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="ACHBFA_002301"
     CDS             complement(3821..6268)
                     /locus_tag="ACHBFA_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9395732.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="ACHBFA_002302"
     CDS             complement(6457..7893)
                     /locus_tag="ACHBFA_002302"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9395733.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2215 a         1736 c         1358 g         2670 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa atatgtaaa
//
LOCUS       DAYYNG010000132         7971 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000132 DAYYNG010000000
VERSION     DAYYNG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.133"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..714)
                     /locus_tag="ACHBFA_002303"
     CDS             complement(151..714)
                     /locus_tag="ACHBFA_002303"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9395734.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(729..1772)
                     /locus_tag="ACHBFA_002304"
     CDS             complement(729..1772)
                     /locus_tag="ACHBFA_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395735.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDKKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMQTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1905..2309)
                     /locus_tag="ACHBFA_002305"
     CDS             complement(1905..2309)
                     /locus_tag="ACHBFA_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9395736.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2477..3112)
                     /locus_tag="ACHBFA_002306"
     CDS             complement(2477..3112)
                     /locus_tag="ACHBFA_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9395737.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLGSMIFGVLTRLE"
     gene            complement(3378..4430)
                     /locus_tag="ACHBFA_002307"
     CDS             complement(3378..4430)
                     /locus_tag="ACHBFA_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9395738.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4443..5381)
                     /locus_tag="ACHBFA_002308"
     CDS             complement(4443..5381)
                     /locus_tag="ACHBFA_002308"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9395739.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5832..6482)
                     /locus_tag="ACHBFA_002309"
     CDS             complement(5832..6482)
                     /locus_tag="ACHBFA_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395740.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6582..7091)
                     /locus_tag="ACHBFA_002310"
                     /pseudo
     CDS             complement(6582..7091)
                     /locus_tag="ACHBFA_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
     gene            complement(7454..7837)
                     /locus_tag="ACHBFA_002311"
     CDS             complement(7454..7837)
                     /locus_tag="ACHBFA_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395741.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNKVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         2470 a         1646 c         1227 g         2628 t
ORIGIN      
        1 tctagatggt cattcatgtt agaagaatca aactattttt ttactgtcgt aataatatta
       61 aaacactaga acaaatcaat taaattccat aaaatataaa taatagacct agcatcatct
      121 ggtataaaat ggatgctagg tccgttatat ttactcaaaa ttgattctac gaatagatct
      181 tcttatcatt tctattttag tagtcatgcg ttgttgttct tctaaaggtg gtaatggtat
      241 aagtagttta gataaatttg taccatttac atttggttgc ccagttccgt aagaaatttt
      301 ttctaataac ttccaatata atggcgagtc taagaagcag tctacgtatt ctgataataa
      361 tttctcaact aacctaattc gaatcaagta agaagcaaaa acagattcct cacttatttc
      421 cttaaccaag aaactttttc ccatagttcc tcctgtcctt gctatcaaaa tgtcattttc
      481 ttccaatctt aaatcaacta gtttagaatt actaatatca cagtaaggca cactggacca
      541 atttacacgt ccctcttgta tatctgtgat tctcaaaaac ttaacattgc cttgtttttt
      601 cgccgaatct gtatagccat attgaatctt agtagaaata cttcctaaat aaacccatag
      661 tcgaacatcg ctagatgatt tttcttgtcg ttctttatga acaacagtga tcatcttgag
      721 ttgtccgact attttttagt tgcagcaatc tttttgggac gcttatttgc atgaagcaac
      781 gttgctaagt ctagtaataa aacattggct aacacaggat tggctttctc gcgatacgct
      841 gcggcgccac tttcaaacaa tactgctaga cgatcttcta caacaacgat ttgttccaaa
      901 tacggtggaa atttgattcg ttttaggatc cggctaggag agtcccgctc ggacactaag
      961 agttccgagg tataaggacc ataagaacga ctggctaaga gataatggcc ataaaaagcc
     1021 acaccttgaa tcttatcaga tgtttttctg ctcattgtca cggttggttg gattttgtct
     1081 ttgcggcgaa ttgctgtttg catttcagct atcgtcggca atagatagaa agtcaactgt
     1141 ccttcacctt ttagtgtgaa gttgccagat accagttgat cattgttttg agtcaaatac
     1201 gatgcttgtg gaatctctga gagatcagtt gtcagccaat aagcgattgg ctgtcccgtc
     1261 tcttcagatg tatctgctaa gatatcgatc aaacgtaaag cggctactcg tccagtagca
     1321 gatcctgtga ttgtcaacca taaaagttcc cgttttttat catatactaa gccccctaca
     1381 tgtggccgtt taggtaaaat cagcagtttg atcggctgtc ctgtttctct atccagtaca
     1441 tgaataatag aatgatgctg atggtcatag cagtaggcag aaataaacac aaaatcttga
     1501 gaaaaagcca acccttgcgg cgtcatccat tgacaaacct cattttcgcc ggtttccggc
     1561 accaccattc ttgtttctgt taatccagga atgttatatt gttttgcttc agccttttcc
     1621 caagctgctt gaacaatagg ataccgtttt aacttttttt caatatcctg atgtatccgt
     1681 tgctgcttcc aatgcttaaa ctttaggaca tagtaaacaa ggaggccaat gaccagcaac
     1741 actaaaatca ttattgctac ccgctgtgtc atttttttgc ctccttgttc tattcgatca
     1801 atcgtacata taccgctatt aattaccgta actacatgtt tcttttacta gcgtttcttt
     1861 ataggatctt tcataatctc atttatagta acattaagaa tcccttattc tactatagtc
     1921 acttccacat caatattgcc tcttgttgct ttagagtatg ggcaaaatgc gtgggcttct
     1981 tctgcgattt tctctgcttg gtccttctca agtcctggca aagaaacttc taaaacaacc
     2041 ccgatttggt agcccccatc ttctaattga aataatgata cccgtgcttt gacttcagaa
     2101 tcagactcaa gattattttc ttccagcatg tgttgaaccg cgccgttgaa gcaagatgcg
     2161 tatccagccg caaaaagttg ttccggattt gtcccttcac tatggattcc tgggcggtcg
     2221 atcttcatgt gcatacttcc gtttggtgcc tcaacctcgc catcacggcc tccacgattg
     2281 atcattgctg tttcgtacat ttttttcatt gagatcatcc tttctttcgt cgttctatat
     2341 taagtgtaac gagtattttt taatctgaca aaggtaatgc ttgcattcgg cgacatcagt
     2401 tagacaacat cgatgatcag tgatagaagc agtttatcta tacaaaaaaa gaagggttct
     2461 atgatatttg gtgttgttac tcaaggcgag taaggacacc aaatatcata gaacccaaat
     2521 agttaaagaa aaaacttaat cgttgaaaga agcccaattg cttaaagaag gcaaactgac
     2581 taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta ataactgtta
     2641 aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc agaacaagta
     2701 acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta tggatccaag
     2761 tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta aaaatacaat
     2821 cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt tttgatgttc
     2881 caacaaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca gatacaactg
     2941 accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca ctaattacgg
     3001 tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc gataaatcac
     3061 taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc attttcttcc
     3121 cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttcaca ttttaacaca
     3181 tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt tcttctacat
     3241 tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc tactcaaaaa
     3301 ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat tgattgaaaa
     3361 tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc atacagttta
     3421 aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat gactaaccaa
     3481 caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg accaacaagt
     3541 ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag agcgacagga
     3601 atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt tctaaataga
     3661 ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc tgacttatta
     3721 accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat cgcagccgca
     3781 ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga aataggagag
     3841 ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg ttgtaaggta
     3901 gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa agggaataaa
     3961 taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac aatcgacact
     4021 gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc tgtcagcatc
     4081 gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc caaatcaggt
     4141 gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat cattctcatc
     4201 ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt tgctagttga
     4261 aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa atattttaaa
     4321 atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc tgtaccagcg
     4381 aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat tcaatctctc
     4441 ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa gaaatcacaa
     4501 taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt gatataatcg
     4561 atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata atgtaaccca
     4621 attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac atctagttca
     4681 atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac cattttatga
     4741 gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca tagtccgttc
     4801 attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc agaaaaatga
     4861 gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc ttcttttcca
     4921 tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg accaggacct
     4981 tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg tacaaatttc
     5041 tcaataatat cttcatgccc gataccattt aatagacata agacctctgt atcttttttg
     5101 attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt aaatagaata
     5161 atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg tagttttgct
     5221 tctacctctt taccattaaa atttgcttgt aatccatgtt ctttgatttg ttggacatgt
     5281 tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat taacccaaag
     5341 cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc ctcctttttc
     5401 tttctattat acttatttga gaatcatttc acaaccttgt tttgttattc ttctcttatt
     5461 tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa aactggaata
     5521 gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga aacattcata
     5581 gaaaaaacct cactcctctg attataaaag aggggacatt aacctcgtgt atactaaaac
     5641 cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg tagtacgatg
     5701 ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca atacacttat
     5761 ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt attatcatga
     5821 aaaatagcct tttattgtct atgggaataa taatttaaaa ttggtacttt tttattaatt
     5881 agttcatcgc ccaataatct ctccagtttc ttagcatctc ctgactcaac cggaattatc
     5941 caagcccagt ccaaataatt ttttaaaatc tcatcatatt ttgcaaatat ttcattgcca
     6001 tattccgtcc ttaaaattat attttcagtg ccagacaagt gatttgtatt gagttgataa
     6061 aaaagatctc catctaaaaa aatatcatta gaagaagatt tccccacagt aatgaccatt
     6121 ccatcagctc tatgtgttac atacaaataa ttgtatgacc tcacttcacc taatgaatca
     6181 attatttctt gccctttagc tctaatatat tgatctctat tacctatctc actcttattt
     6241 aacaaaagtt ttttcatgtc aaatttaatc ggctgtttta tttttgtaaa ttcgcttcta
     6301 ctactaatcc aatccgagat taatttttca acatgtccca gagtaaactc tgatttattc
     6361 gctaaatact ttttttgttg tattaaagac atctttcccc gaagctcaaa attgagccta
     6421 catcgattct ctatctcttc aaaaagcatc ttatcttcat cattcttata ttcatcattc
     6481 ataaaaccaa tccagctcct cgattaagat aatattttaa tctcatacaa aaactagctc
     6541 tcatcaaaac aaccattgtt ttgatttctc atgaaatttc tttatacttt tctccgatta
     6601 taaattccta accccttgag aaaattacgc atatatttat ctccgcaagg cttaaaattt
     6661 cgttggcctt cttttctcaa aaatgccccg atttcgcctt ttgatggata gatttctgcc
     6721 tcagcaaaaa cgtctaaaat atcatcagtg gtcatagaca aggcgatttt taattttttt
     6781 agcaacacat tgttgatata tttgatttcc ttgctttttt acggcgtgtc tactatcggc
     6841 ttatcttttt gcggaccacg tgccaagaga atcaggccat ttaggaatgc gtcaaaaact
     6901 tggttgttga tggttttttc atacacatta ttttctgcat tatctttgtt cttatcctgt
     6961 actttggtca gtaccgcctg agcttcttct ttagtcagtg gtacaccgcc tagctcaaaa
     7021 gctttaatca aatccgtatc tttcaaatca atcgcataac gcaatcgcaa taacctatca
     7081 ttgttattca tttgttcctc cgaagtattt agtctttact ttcttacgta ctttttgtct
     7141 tgctaagatt tttaggatca atgataaaaa ccgcatcact tatgatacgg agctgggtat
     7201 catgagtgaa tgatatttat aaatcactgg atattgaagt atattctgtt ttctcaactt
     7261 atttagtcag attttttaat gaagattttt tgagtaaaat ttctataatt cctacttatt
     7321 gaaaagtagt ttgtagtgct gcttctacct ctctcatctg ttctacaaat tccaaataac
     7381 ttgacgcagc ttgcggaatg aaaaaatgat atgtgaatat ttctcctcct agcccgctta
     7441 atatgcctat tttttatacc catgatttgc aggaagggga tcggaagtag gttgcagaaa
     7501 gccgcctttc ataatctgtc ggaaaccgga catcaaaacg gattatttta cttgtcacat
     7561 ttgtattttc ttgtgaggaa gtgccaaata ccttatttac ttgagtgaca ttctcattgt
     7621 cctgtctgat attgcttcgt ataaaagtac cagatccacg tctcgtatat aacaaacctt
     7681 catcaataag gagttgtata gctttgcgga tagtaacacg gcttgtttta aactctctag
     7741 ccagactttc ttgctcaggt aatttagaac cttcctgata ttttccctca acgatcctct
     7801 cttttagagc caaggctatt tctttatatt ttggcatatt ttcccctcat ttcattgatc
     7861 ctacaaaaaa attagtatat atacaaattt ttgtaaagta tatatactaa taaatacaaa
     7921 cccgtggtgc tagttaattt taatatccta aagaatatct caaagttcct t
//
LOCUS       DAYYNG010000133         7938 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000133 DAYYNG010000000
VERSION     DAYYNG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.134"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..383)
                     /locus_tag="ACHBFA_002312"
     CDS             complement(<1..383)
                     /locus_tag="ACHBFA_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395742.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGK"
     gene            complement(417..770)
                     /locus_tag="ACHBFA_002313"
     CDS             complement(417..770)
                     /locus_tag="ACHBFA_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395743.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(882..1283)
                     /locus_tag="ACHBFA_002314"
     CDS             complement(882..1283)
                     /locus_tag="ACHBFA_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395744.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(1320..1829)
                     /locus_tag="ACHBFA_002315"
     CDS             complement(1320..1829)
                     /locus_tag="ACHBFA_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9395745.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(1851..2708)
                     /locus_tag="ACHBFA_002316"
     CDS             complement(1851..2708)
                     /locus_tag="ACHBFA_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9395746.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(2726..3559)
                     /locus_tag="ACHBFA_002317"
     CDS             complement(2726..3559)
                     /locus_tag="ACHBFA_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9395747.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(3573..4370)
                     /locus_tag="ACHBFA_002318"
     CDS             complement(3573..4370)
                     /locus_tag="ACHBFA_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9395748.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(4403..4687)
                     /locus_tag="ACHBFA_002319"
     CDS             complement(4403..4687)
                     /locus_tag="ACHBFA_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM9395749.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(4684..5685)
                     /locus_tag="ACHBFA_002320"
     CDS             complement(4684..5685)
                     /locus_tag="ACHBFA_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM9395750.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(5687..6589)
                     /gene="gnd"
                     /locus_tag="ACHBFA_002321"
     CDS             complement(5687..6589)
                     /gene="gnd"
                     /locus_tag="ACHBFA_002321"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM9395751.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            6753..7601
                     /locus_tag="ACHBFA_002322"
     CDS             6753..7601
                     /locus_tag="ACHBFA_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9395752.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         2321 a         1717 c         1275 g         2625 t
ORIGIN      
        1 tcttttcctt gtttgacaat ttgttccacc gcttcttcta tcggtgtgtg aagcatctga
       61 tgattgattg cttctaacaa cattggcaga ttcacgcccc ctatgacata agtggctgct
      121 ttcttttctt cagtcagttc attgactgcc aagagcgctt gattgtacgg acttgcgcta
      181 attagatcaa caaatactag catgggttct gctgtgttga tcatagttaa tttttctttg
      241 accttctctc cgaatgtttg gatgtcctcc ccagactcta agcacattgt ttcaatatgg
      301 ctcgatgtcc cgacaattac ttccgccgca tctttcagtc cttcgctcaa cttaccatgt
      361 gtaacgatca tgatttccaa cattagtctt ctcactcctt gttttgcact ttttacttat
      421 cgaaaaagag caataatctg attggcattt tgccaattaa atgttttccc tcgtaaatct
      481 gttttggtca aatagaagaa ctgcgcttgc ttttttcttg cactaaaaat ataattgacg
      541 tttttttctt tcaacttatc aacttgtttc agccactgtt ctttatttga aaaataattt
      601 cccgaaaact gtagcggtgt atctgcccca acagagtcta gataaaaaag aatagcctct
      661 tttttcatac ccgatctcac gctgtctaag cccttttctc cataacagcc ttcatcaaca
      721 aacgcatacg ctacactcgg atcatcaatc gtacggatca tttctagcat cgttaatatg
      781 gatgaagtat ttcgaataaa agtttgtctg ctaggccatc cgcgagctgc ttttcccaat
      841 aagaaaaagt agctcccaaa tgctagtaca gtgatacctg tttatacgga tagcagatcg
      901 aatacgtgat gaggattttt gttatatatc atcattcccc accaacctaa actgaacaac
      961 aaaatgaagg acaataatat agagtagatt gtttttttcc tttggtcttt ccaatcaaac
     1021 atgaaataac ttcctaatgc atgaacgggt gtatcgtaat aagtacagat aactttctct
     1081 gctttttcga tatctccaac ataaatattt tttgatcggt aggctagcgt atcgtaagca
     1141 atgactgtca catctgttct catactgtaa atatctgata ctaatgctga aagaaaacgc
     1201 tgtttactct tttttgtacg gcgcttacgg taaatatagc gataacgaaa ttcccaatca
     1261 cgaaacagct cgttcttctt cattttttcg ccctctttat cttttttcta agcatttgtt
     1321 tatgacaaat caaacttgtt caatacatct tttaatcctg ttttaggtgc agatggagtg
     1381 gcttgaaaat aaatattctt tacgccatat tgttcattca tttctttcaa tttttcagca
     1441 tctgatttat ttaaataaac agatttcgtc actaaaagat ctgtatcagg atttttttga
     1501 gcaatccctc caatattcaa ttcttccatg gggattccgt gttttactaa gtcgtaaatc
     1561 gtttggactg tttttgcgat caaaatacaa ttgtattctt taaattgata ttcatcccaa
     1621 tagaatttag ctttctctgg tgtaatgaca attgtttttt gtcctgtacg gcttcctgca
     1681 tttttgtaaa gatctctcat aaatttatct tttgctacaa cttcgtctac aacaaaaatg
     1741 gaattcactc cacttttttg agataacgtt accattactt gcccatggat caaacgctca
     1801 tccacacgcg ctaaattgac tacacccatc ttattcacgt tcccttcttt ttataaaata
     1861 ccgattcctg ctagcacaat taatattcca gtcagccata ataatgcttg tgtgactttc
     1921 aatccttttt tcgtatagaa caagtacaca cccattacga cagctaaagg taatatccct
     1981 ggcacgattt gatcaagaat ttcctggata acaaactcac gtccggatat cgcaaatttc
     2041 aatgaagatt ctacttttac atagtttccc gcaagcaccc ccatcataaa caaccctaaa
     2101 actgatagca tttcaatcgc tgtctgaaca ccagtgtttt gcattaggcc tgtagcatta
     2161 cggcccatag tgaaagcttg tcttgcgaaa aataccccta gtaaaacctg atacaaccca
     2221 aagcagataa aaggaaaaag tgcgcctaaa ggacttccct gttgtgccca aggtaccgcg
     2281 attgcgataa aaatatattg gacggttcct gaatcgattg catctccgat tcctgctagt
     2341 gcccccatca atcccgcttt cgtgttatac atcaagtcat cctgcatcaa gatctctttt
     2401 tcttcatact cttcttctgc tctttgctgt tccatagaag acattaggcc agtaatcaca
     2461 ccgccacccc agcttaattg agtgttgaag aacagtaact gccgtttata tgctgctttt
     2521 aaagagtctt catctttata aagttttttc aagattggca tcattccgta tagtagtgat
     2581 ggcgccaagt aacgttcaaa agagtgagga atttcgtttg caaaatgcca tctcagatag
     2641 gcttttgtta catctttttt cgtgatttct tcaggagcac ctgtttgatt atccattatt
     2701 tcagccattt cccattcctc ctaaattaaa atccgtcatc atagtcatct tcgtcatcaa
     2761 aagatgctga attgttattg cttgtggcag gctgtacttg tttccctttt tgcgactgga
     2821 caaagatcaa agcgataata atcccaaaaa tggcataagt aaccatcgta atatctaaag
     2881 atttcaaaac aacactcata aaataagcaa ggaagaaaaa gaccagataa tcttttcgac
     2941 cgataacgta tacagttgtc gcgataccga ttgccgctaa ccctccacct actacttcaa
     3001 gaatgtggat aacgactgtt ccctctaaag ctttgattac atcagcgatc aatggcgccc
     3061 ctaaatataa agcaatgaaa acaagcggaa cgaataataa aaatgaggca accgttggat
     3121 aaagaataat tgatcgggcc atagcttttg tattcaaatc ttctacagct ttgtctgtat
     3181 atttacctaa aaaagtattg atgaaaaaac gaaactgata caagtaactt ccgagcaatc
     3241 cgactggcac cgcaacggca atcgctgctt ctggcgacag atcccctaat aatgcgactg
     3301 gtactgcgat agctgctgca atagatggtt cagctggcat tgatcctcct ggagaaaaaa
     3361 cccccatata aatcatttgc aaagcggctg taacaatcat cgcttgtgca acttccccca
     3421 tgatcaaccc gacgactaat ccagtcatca aaggagaaaa acgtaaggtc aacgtggctc
     3481 ctccacctaa ccagcgggac ataaccgcgg ctacccataa agcaatcaac aaactttgta
     3541 ctacacttaa tgtttccatg tgtcctcctc cttcagatct ttctgatata accttccagt
     3601 tccatcattg actgtatcag tgatttctgt gttacctcta taggaatcaa atgcactttc
     3661 tctttcgaat caataaattc agccatttta tctattatct gtttatcttt cggaaataat
     3721 cccatttgtt ctagagaaac cggcagttct aattcccgat acaaaggata taactcgtct
     3781 attttttccc agcgaccttc tagtgccagt tgatagaaaa tcccatacgc tactttttct
     3841 ccatgtagaa aacgatggct ttccggaata tatttactga tagcgtcatg catagcatgt
     3901 gctgccgcat ttcttgcgta tttatctcct aatcctccaa ctaaaccagc aacagcaaag
     3961 ataatctctg aaagctgttt gaactccgat gtcagttttc tttgtgccat atcttggatt
     4021 gctttagcag aatgattcaa aatctcttct ttgcagatct tcgcagtatc ttgtgctagc
     4081 ttcaaaaatg attcatcttg caaaaaatct tgttctaaga tgagctcaga ttcataccat
     4141 tttgctagtg tatctgctaa cccggcaatg aaatatggaa caggggaatc aatcactaat
     4201 tggggatctg ttataaaaaa agcagcttgt ctgtaaaagt gttctgtttt cccttctgct
     4261 tgtccattct ccttatacat gacagcaaga ggtgtccatg gagcacaatt actagctaaa
     4321 gtagggatga ctccgaaatc gattcttgca agattggcac tataacccac tagatcggtc
     4381 agttttcctc cgccaacgcc aactacgaaa tcgatttcat ttttttgatg attgtagcta
     4441 ttcgtctggc ttcagcataa ctacattctc cagaataacg atgataaaaa aattgaatcg
     4501 tttcgtcatt caaaaatact aatttaggta gcgccttttc ccatgatacc gttccgtggg
     4561 tgaccagtac tcttttcgcg ccgtatgatt tcaagagatc agaaactttt gaaataccac
     4621 cttcttcata taaataaaat tgagggcctg ttttcacttt taaaccgttc accattttct
     4681 tcatcatcct tttatatagt tgctctttgt ctggacagaa cgttcaggaa gaaggtgctg
     4741 actgatctgt tcgacatctc tgacacattt ttcacccatt gcttctaaac attcccttgt
     4801 gtaggccgat atatgtggcg taatcactac ttgttcaaac gctaaatacg gatgatcttg
     4861 acgtcccggt tcttcttcta aaacatctgt ggcatatcct gcgatctttc cgcttttcag
     4921 accattaatc atcgcttctt catcaactag agcgcctctt gcactattag atagataaac
     4981 attttctttc atctgatcga cttctttatc agaaatcatg tgatagttct cttctgtcaa
     5041 attcgcacat aaacaaatga tatctgattg ttccagtaat tcttgaagtt ctactttttt
     5101 tgcaccaaag ctttcgatgt ataaatctga tttgtaagga tcataagcta gaacatcaca
     5161 gcgaaaaccg ttacgcaaaa tttcgattac tccgcttccg gtatttcctg caccaatcac
     5221 accaactgtt ttgttaaaca gcgtcctacc gacgaactcc gcccgatctt cccatcgatc
     5281 gtccttgact cttgcttgtg cttgtgtcgt gcgtcgaagc atggacaaca aattagttat
     5341 attattttct gctacagcat ttcgttcaac taaagcaggt atgatagata cgattgtctg
     5401 gtgttctttt gctgctttta aatcaatatt gttgtaacca ataccatgtc gggaaatcaa
     5461 tagcagttca tctttgtttt caaaaaaatc tcgtcgaaaa aatggcgtaa cacttgcaat
     5521 aatgatattg aatcctttaa gtaactccgc tagttctttt cctggaatct cttgatcaac
     5581 agtaaagaaa tgaacgtctc caactgactt taatctttcc aagtggtgtg gaaagactct
     5641 tccgaaactg ctggaattaa cgattgctat atgataatct ggcattttac tcaccctttt
     5701 caactatttc atgtccgcca aacccatttc ttagtgctga aactactttt gctgaaaaac
     5761 tatcttcttc ctgtgaaaga ttcctcatga acaaactagt tgcaattact ggaactggaa
     5821 cagccatttc tagcgctgtt tcaaccgtcc attttgcttc acctgatgca gaaacgattc
     5881 cccgataatt acttaaattg ggatgttcgt ttaactgatc ttccatgatt tccatcagcc
     5941 agccgcgaat caccgaacca tgattccaaa cacgtgcgac ttgttccaag tcaaaagcat
     6001 actcactttt ttgtacaagt tggaatcctt caccgattgc ttgcatcatt ccatattcga
     6061 ttccattatg gatcattttc aagaagtgtc cgcttccgga tgcaccagca tacagatatc
     6121 ctctttcaat tgctacatct ttgaataatt gttccacgga tagaaaagtt tctttatctc
     6181 ctccaatcat taagcaggct tgatgtcgtg ctccttcgat tcctccagaa gtcccacaat
     6241 ccaagtaacc gatttcattt ttctgaaact cttctgctcg acggattgaa tctttataat
     6301 aagagtttcc gccttcaata ataatgtctc ctggtccgag ttttggaaaa agttttctta
     6361 acatgttttc ggttatctct ccagcaggaa gcattaacca gatgattttt ttctgctcaa
     6421 aagtagctag aaattcagag atctcttgat aagcaggaat gtcattttct cttgcctgac
     6481 taacagctgt acttgaaatg tcgagtcctt gtactttgtg atggtgctct tttaagttta
     6541 aagctaaatt caatcccatt tttcccaaac caattacacc aatttccatg atagcgcctc
     6601 catttcttat accctcatta tacaaaaaaa tttttttgta aatagatttt acaaaaatat
     6661 tttttgtttg attttttcct actctccttg tatctttaac attaatgaga acggaaaaaa
     6721 tgataatctt attatgaaaa tgaggaattc tcatgagcgt ctccataaga ctacaaatca
     6781 aaacaatgta caacgacttc agcccaaaag aacaggctat cgccgattat attttagaaa
     6841 atccaagcaa agtaagtcat agtcctatta gcaatttagc aagtgaactg ggcattgctg
     6901 actctacttt ttttcaattt acaaaaaaac ttggttataa tggatttaaa gattttaaaa
     6961 tggccatgtt gatccaagaa aatgatctat ctgctatttc tatccacgaa aatattcaga
     7021 aaacagatac tgagctcact atggcgcaaa aggtgttcga gtctagtatt tcaactttaa
     7081 atgacacaaa aaagttgtta caacaaaagg atctaaaaaa agcggctact attatcaatg
     7141 aatctaaacg tgtctatttt ttcggtgttg gcggttcaga gattgtagca actgacgctt
     7201 atcataaatt tttgcgttcc cctatcgcaa cttctcacag cacggattac catattcaat
     7261 tgatggaagc ttctttattg acggaaaaag actgtgctgt tttgatttct cacacagggc
     7321 aatcaaaaga aacaatccat attgctgaaa ccgtaagaaa aactggagca aaaacaatcg
     7381 ttattactag ccaagctaat tcttcccttg ctaaacttgg cgatgtcgtt tttatttcta
     7441 tttctgaaga aacagaattc cgatctgaag ctttagcttc ccgtatttct caactaagca
     7501 ttttggactc tctatatgtg attttgatgt tttacaacga aaaagaatca cgtcgttcta
     7561 tcgctaaagt tcgacatact atttctgaaa tcaaagaata gcaccataac agaaaaagcg
     7621 aaaaatccat aaaacaaaaa aagccactac caaatatttg gtagtggctt ttttggttct
     7681 gcaagggtgg tacagaacat caatagtcgg tgggttagaa cgtatgtcat cgcatctttg
     7741 gagaaggttg tattggtatt attcgagtta cttgttagat gcgataacaa agttagtata
     7801 cagtagtaaa aaatttatct tttctttttt ttattatttt tgtttcttat tttctaaaaa
     7861 attaaaaaaa attttggtga attaagtatt tccttgataa cactggcttt tggcctcatt
     7921 ttaggttatc atttgtaa
//
LOCUS       DAYYNG010000134         7872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000134 DAYYNG010000000
VERSION     DAYYNG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.135"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..421
                     /locus_tag="ACHBFA_002323"
                     /pseudo
     CDS             1..421
                     /locus_tag="ACHBFA_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769456.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
     gene            470..1483
                     /locus_tag="ACHBFA_002324"
     CDS             470..1483
                     /locus_tag="ACHBFA_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9395753.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1550..2584)
                     /locus_tag="ACHBFA_002325"
     CDS             complement(1550..2584)
                     /locus_tag="ACHBFA_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9395754.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2698..4923
                     /locus_tag="ACHBFA_002326"
     CDS             2698..4923
                     /locus_tag="ACHBFA_002326"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9395755.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALIHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHSDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLITDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            4941..6824
                     /locus_tag="ACHBFA_002327"
     CDS             4941..6824
                     /locus_tag="ACHBFA_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM9395756.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGTWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSTAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGYFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VYLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPEGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6805..7779
                     /locus_tag="ACHBFA_002328"
     CDS             6805..7779
                     /locus_tag="ACHBFA_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM9395757.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2546 a         1446 c         1679 g         2201 t
ORIGIN      
        1 atttgacgcg gaaattatta ttcgatttgg gcggcatcag ggggttatga cgacgtagca
       61 gacccattat tttactcaag aaaatgtccg tgcctactat ttagaatacg atagtgaacg
      121 tgctgggagc tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      181 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      241 aagcgacacg atatctgcct ttggatcgtt tgtgattaag tcctcaatgt ggatttgctt
      301 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      361 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      421 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      481 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      541 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      601 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      661 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      721 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      781 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
      841 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
      901 gaatgaactg attaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
      961 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1021 acaaacgaat gacgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1081 tctgaaagga accatttctt attgcggacc tgttgatttg aagctaacag cacagcagca
     1141 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1201 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1261 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1321 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1381 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1441 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1501 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1561 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1621 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1681 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1741 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1801 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     1861 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     1921 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     1981 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2041 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2101 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2161 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2221 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2281 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2341 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2401 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2461 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2521 acacgcggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2581 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2641 cagtatacag gaattataaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg
     2701 gatattacgc atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc
     2761 ttccatttac gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg
     2821 gcgcatattt attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga
     2881 agagatcgtg gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat
     2941 acactaccgc aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc
     3001 attcagcgca agaatggatc agacttactg gatctgagat atgacagcca ttatattaca
     3061 gatgggaagc cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca
     3121 caaacgttgg tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat
     3181 accatattta ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga
     3241 gaaacaatca aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga
     3301 tttgatgaag tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa
     3361 tctgttttat ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg
     3421 aataacttta ttgctttaat tcatcatcat acgacagaaa atactggaga agcgatcgga
     3481 cttcaatttg tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa
     3541 ttgagagtcg tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag
     3601 tcttttcaaa cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt
     3661 cagatccacc atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa
     3721 cggccgattc tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc
     3781 aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc
     3841 tggtttggaa aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc
     3901 aagttgacaa atggattacg tgaaatagca gattatgctc atagcaaagg gttgaaattt
     3961 ggtttatggt ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacattct
     4021 gattttctta tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg
     4081 gattttacaa ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat
     4141 acgattcctc ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg
     4201 attacccttg acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat
     4261 gaactacttg aacatttgat aactgattat ccggaaatct tatgggaagg ctgttctggt
     4321 ggaggtggac ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat
     4381 aacacggatg cagtggagcg tatgaaaatc caatatggaa caagtcttgc ttatccgatt
     4441 tcgtccatca cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg
     4501 ttgaaaacaa gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca
     4561 aagctgtctc ctgaagaaaa aaagcaagtc aaagaccaaa ttatatccta tcaaacgatt
     4621 cgtccggtga tccagtatgg ccattattat cgtttggcga gtccttttga agaaaatatt
     4681 gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt agggaggata
     4741 ctagcttcag ctcaaccagc gttccatgaa gtatatctga tggggttaga tgatgaggca
     4801 ctttatcagg aacagacctc gaagcggata ttttcggggg ccgaattgat gacagtcgga
     4861 ctttacttcc cagattttca aggtgatttc caaacagagc tgcttcattt caaaaagtta
     4921 tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg gttagatgat ccacaagttt
     4981 ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg ctatgcaaca agggaagaag
     5041 tagaaaccac atcctccctt aaacagtctt taaatggaac atggcgtttt tgttattcgc
     5101 caaatccttc ggtacgtcca aaaacatttt ataaaaaaga ttttgatgcc tgttcttttg
     5161 atgaaatcac tgttcctagt catattgaat tagcaaatta tgacaagatc cattacatca
     5221 atacgatgta tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg acaagccctg
     5281 agaaagaggc ggaagggata ttttcaactg ctaaagacaa tccggtgggt tcgtatttga
     5341 cgacttttcg attagaagaa ggtctaaagg gaaaacaaat ctcgattcat tttgctggtg
     5401 tagaacaagc aatgtttatt tggttgaatg gttattttat cgggtatgcc gaagattcgt
     5461 tcactccgtc tgaatttgat ttaacacctt atttggaaga agaaaatatt ctagctgtgg
     5521 aggtttacaa aagaagtaca gctgcattct tggaagatca ggactttttc cgattttttg
     5581 ggatcttcag agatgtctac ttagttgctc gaggacaaaa ccatttagaa gatctttgga
     5641 tcaaacctcg attagatgac aaatgtcaaa atggcaaact tgaagtagat ttgtcttttg
     5701 agcaagtagc caaaaacagt cagctgaatg tagcgatact agatcctgag gggaaggaaa
     5761 tctatcaaca gcaatatccg ttaaaagagc aaatgacgat tcggatcgat ggatttgaaa
     5821 acgtggcact gtgggggcat tttcagccca atttatataa gcttgaattg gagacgatag
     5881 acgaagaaaa aagagtaacg gagtatattg actattcctt cggttttcgt aaaatcgaaa
     5941 tcaaagataa aatcatctac ttcaatggaa aacggatgat catcaacggg gtaaatcgac
     6001 atgagtggca tccaaaaaaa ggacgcgcca tcactataga agatatggaa aaagatttgg
     6061 ctatcataaa aaatgcaaag atcaacggcg tacggacatc tcattacccc aaccagatca
     6121 cttggtatta tttatgcgac caagcaggca tccatgtgat ggcggaaacg aacctcgaat
     6181 cccacggctc atggcaaaaa atgggcgcat ttgagccttc ttacaacgtg cccggtgcag
     6241 tacctcagtg gcaggctgct gttcttgatc gggcaaaaac gaatttcgaa acattaaaaa
     6301 accatccaag cattcttttt tggtctcttg gaaatgaatc ctatgcggaa gaaaacattc
     6361 gacaaatgaa cgattacttc aaaaaagtag accccgatcg actcgtccat tatgaaggga
     6421 attttccaaa tccagcattc gaagattcga tttcagatgt aaaaagctgc atgtatgcgc
     6481 caccagaaga gatcattgac taccttgaaa ataaagcgga aaaaccgttc atcttatgtg
     6541 aatacatgca tgacatgggg aattcgttag gtggaatgga ttcttacatg acgttgcttg
     6601 atcgttatga aatgtaccaa ggaggctata tatgggattt taaagatcag gcgatccaag
     6661 tgactgatcc aatcactggt agagaagtga tgcgctatgg cggtgacttt gacgataaac
     6721 caagtgacta tgaattctca gggaatggaa tcgtttttgc agatggacaa gaaaaacccg
     6781 ccatgcagga ggtaagatat tattatgaaa aatacagtaa ataaaagtca tatggatacg
     6841 gaaaaagttg caatcgtctt cggcgactgt acattaggtg tcaaatcggg gaatacgcat
     6901 tatatttttt cttatacaag aggcggactg gaatcgctca ataaaaacgg aaaagaatgg
     6961 ctataccggg aaacacttcc agttttttgg cgagcaacga ccgataatga cagaggtagc
     7021 ggatttggat ttaaatccgg tatgtggtta ggagccggat tgtatccaaa agtcaaagaa
     7081 atagaagtac tggtggacaa taaaaagatc gatttgccga ttgctccagt aaataatcaa
     7141 tattcaagta atgaatacgc tgaaacggtt gaaatcattt ttacactgga aatcaacaca
     7201 gtaccaaaaa cccaagtgac aatttcctac caaatcgctg gagagggtaa aatgcttgtc
     7261 aatatgcatt attatggaca aaaaggatta ccggaattac ccgcattggg tttaaggttc
     7321 attatgccaa cgaaagcaaa attttttgag tatgaaggcc tatcagggga aacatatccg
     7381 gatcggatgg ctggtggtgt cgaaggtatt tataaagtgg caggactccc cgtttcccca
     7441 taccttgttc cccaagaatg cggcatgcat atggaaacga aatgggtaga actaacaaga
     7501 aatcaaacgt tggataatac ggataaagat caagaagatt tttcgttgcg atttgaacag
     7561 caggatacgc catttgcttt ttccgcttta ccattcaaag cagaagaact ggaacaggca
     7621 acacatatag aagaactacc gccagaaaga cgaacggttc ttacgatttt tgcaaaagta
     7681 agaggagtcg ggggtatcaa tagctgggga gcggatgttg agtcacctta ccgaatcagc
     7741 agcgaagagg accatgaaat cggctttatt attcagtagc aatctttaga ttattatttg
     7801 aaaaacggaa tttacaagac aaagctgctt tattctatat gtcatgaaca agagagtaaa
     7861 aaagttctca ga
//
LOCUS       DAYYNG010000135         7820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000135 DAYYNG010000000
VERSION     DAYYNG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.136"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..170)
                     /locus_tag="ACHBFA_002329"
     CDS             complement(<1..170)
                     /locus_tag="ACHBFA_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit R1E"
                     /protein_id="HFM9395758.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEK"
     gene            complement(329..553)
                     /gene="nrdH"
                     /locus_tag="ACHBFA_002330"
     CDS             complement(329..553)
                     /gene="nrdH"
                     /locus_tag="ACHBFA_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9395759.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            954..1427
                     /locus_tag="ACHBFA_002331"
     CDS             954..1427
                     /locus_tag="ACHBFA_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM9395760.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            1424..3556
                     /gene="feoB"
                     /locus_tag="ACHBFA_002332"
     CDS             1424..3556
                     /gene="feoB"
                     /locus_tag="ACHBFA_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM9395761.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            3558..3692
                     /locus_tag="ACHBFA_002333"
     CDS             3558..3692
                     /locus_tag="ACHBFA_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM9395762.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3786..4430
                     /locus_tag="ACHBFA_002334"
     CDS             3786..4430
                     /locus_tag="ACHBFA_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395763.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4617..5810
                     /locus_tag="ACHBFA_002335"
     CDS             4617..5810
                     /locus_tag="ACHBFA_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9395764.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5803..7530
                     /locus_tag="ACHBFA_002336"
     CDS             5803..7530
                     /locus_tag="ACHBFA_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM9395765.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2526 a         1364 c         1694 g         2236 t
ORIGIN      
        1 attttttcca taatagatgg aaaagtcatc gtatttggtt tgacattttc ctcaaaaaag
       61 gcaactaaag cttctttatc cttattcaaa gggatttgac cattgactgg ccgattgatt
      121 tcattattca gcttgaaata actgacatct tgtaaatttt taagactcat acgtacgccc
      181 aaccttcatt tcgtatttga tgatgcttga ttgcaaatca ttgcattgtt tttaagcttt
      241 aaaaaaggat ttggaagttt gcgacaaaaa ctggtgctgt ctcaagccct tcaaatccat
      301 gtatcacgtt ttgattcttt tagtcatgtt agctggcaag tttacgcaac tgatcaggac
      361 ggaatccgat aactgtttct gcatcagaag taatgacagg aacactttgg aatccttgtg
      421 ctttcaacca gtctactgct tcaggctgtt catcgatatt gacttcttca aaagcaattt
      481 gattttctgt taaaaaccgt ttgaccatct tacattgcat acaattattt ttagaaaaga
      541 tttttacgtt catggtgagt gcctcctcta acacattctt attatctata atagatagta
      601 taaaactctt agtaaaaaag tcaaacaaca aacactacat attgtgttgt ggaaactgtg
      661 gaatactaag ttttgtgttt ttgaaaaaag agctgaaaac aagtatcgtg tgggaaaata
      721 gcaaatttaa tagagaaaaa attgtggaaa actatgaaga aatatttttt tctcattttt
      781 atctatcaat actaaagatt cgctcataat atcacaagta taattgacat tctcaattac
      841 cgagagtatt ctatgtgaga atgattctca tttatttgcg taacacgtaa gaaaattaga
      901 acatataatg aatgatttta atacatgatt tatttcacaa gagaggaatc aatatgcaga
      961 ccttagcgca ggcaaagcag aatttcgttt atcaagtcga acgaatcgaa acagaagaaa
     1021 aaacaaaacg ccatttgcaa aatatcggga tcgttgccgg aagtaaaatc gtcgtcgtca
     1081 atttatcagg caacaatgcc attttactgg taaaaagtca tcgagtggca gtaagtcggg
     1141 aaatactagg tcaaatcttt gtcaataaaa agactgcttc ctctgaaagc tggacttctc
     1201 ttgatcaatt aaaagcagga gagactggga tcgtcgtagc aatccacgga caaggcgcag
     1261 tgaagcgacg gttaatggat atgggattga cgcgaaatgt aaaaatagtt atccgaaaac
     1321 tagcccctct gggagatcca atcgaactga cagtgcgagg gtatgaactg acgttacgaa
     1381 agtcagaggc cgaactaatc ttgatacaaa gggaggaaaa agaatgaata tcgcgttagt
     1441 cggaaatcca aacagcggga aaactagctt gttcaatctc ttgactggtt cgaatcaata
     1501 tgtagggaat tggccgggcg taacagtaga aaaaaaagaa ggatcgttga aaaaaaatcc
     1561 gaacattcat attcaggacc tacccggcat ctattctttg tctccttata caccagaaga
     1621 agtgattgca agagattatt tggttcagga acagcctgaa ctgatcatca acattattga
     1681 tgctaccaat ttagaacgaa atctttattt aactacgcag ttattggatt tagacattcc
     1741 tatgatcatt ggtctgaata tgatggatgt catcaaaaaa gaaggaaaaa aaatcaatct
     1801 agaaaagctt tcctatggtc ttggtgtacc agttttacca atcagcgtat tgaaaaagca
     1861 aggaatcgaa aaactaatcg aaaagattca ggagtctgca aaaaagcggc cttctattcc
     1921 tgttccctgt acatatgatc cacgtttaga agcagcttta catgaaattg aagatgtact
     1981 aggagaacaa agcggcaaga aacgctggta tgcaatgaaa tattttgaac gagatcagaa
     2041 ggtaagagag gattacgaga tagcagtttc tcagcaaaaa gagatcgagc aactgatcca
     2101 gttaacagag aaactattgg acgacgatag tgagacgatt ctggtcaatg aacgttatga
     2161 atttattacc caactatgta cactctgtgt tgtgtcaaat gattctttcc aattatcgat
     2221 gagtgacaaa atcgatcaga ttgcaacgaa tcgttggtta gccttgccga tcttcgcatt
     2281 tgtgatgtgg ctgatctatt atctggctat tcaaacagta ggaacaatgg ggacagattg
     2341 gatcaatgac acattgtttg gtacatggtt accagaacat gttggcagat tgttagctga
     2401 atggcaagta gcgggatgga tgcaggagct gatcttgaac ggcatcatcg caggcgtggg
     2461 agcagtgtta ggatttctac cgcaattgat tatattgttt ttatgcttga gctttctgga
     2521 agactgcgga tatatggcga ggatcgcttt tgtcatggat cgacttttca ggaaattcgg
     2581 tctttcagga aaatccttca ttcctatgct gattgctaca ggatgtggag tgccaggcgt
     2641 catggccagt cgcacgattg aaaacgagca agatcgtcgt ttgacgatca tggtcacaac
     2701 cttcatgcct tgttctgcaa agttgccgat tattgcctta gtagcagggg ccttttttcc
     2761 tcatcaatct tgggtagctc cttctgccta ttttcttggt atgactgcaa ttattttctc
     2821 tggaatcagc ttgaaaaaaa cacgtttgtt tgctggagat acagctcctt ttatcatgga
     2881 attgcccgca tatcattttc ctcaatggtc agcggtccta agacaaacct atgaccgcag
     2941 caagtctttt gttaaaaaag caggaacgat tatctttgtt tctagtatcg tgatctggtt
     3001 cagctcttct tataattttt atgctcaacc tgtttctgaa gaccaaagta tcttagcctt
     3061 tttcgggcga atcctggcag ttgtttttca gccgcttggt tggggaaact ggcaaggaac
     3121 agtcgcagcg attacaggat tggtcgctaa agaaaatatt attggcacat ttggtatttt
     3181 gtttggtcat gcggaagtat ccgaaaatgg cagagaaatc tggcaagtgc tacaacacac
     3241 atatactccc gcagcagctt attcattgtt agccttcaat ttactttgtg cgccttgttt
     3301 tgcggcaatt ggggctatcc atcgggaaat gaatgccaag aaatggactt ggatcgccat
     3361 aggctatcaa tgcggtttag cttatttagt tagttttgtg atttatcagt tgggacactt
     3421 attggagggt ggacaggtag cgttagggac gtatcttgct attttactga tgataggact
     3481 cgtttatgtt ttgttccgtc agccaaaatc caagaaccag ttttatacta tattgtcatt
     3541 agaaggagag gaataggatg gcgacattct tattaagtgt gttaatcttt ggaagtgcag
     3601 gcgcaattgt ctatactcgt ttaaaatccg gtaagaattg tgatgactgt caaacagctt
     3661 gcccagtaaa aaaggaacag atcaagcgat gatcagtcgt tgcaagaggc tattctcccc
     3721 ttttttgctt attttgctag agcatagtaa gatgagggaa agtaaaaaaa gaaagaggaa
     3781 agaaaatgtc tggaaagaaa aaaatcgtcc gcccgcgcta ccagcagatt gctgttgatt
     3841 tagccgaacg tatcgttgaa aaccgatata aagtgggtga gaagatccat gcccgctcaa
     3901 cacttgccag tacgtacaat gcttctcctg agactacccg caaggctatc aatgtgctgg
     3961 tagatcttgg aatcatggaa gtacggcatg gaagtggggc atttgttgct tcgaaggaaa
     4021 aagcaaaaga tttcctattg caatatcaag atgtacaatc catccaagat acaaaagcag
     4081 aaatcatggc aagtgtggag aagcaacaag atgagctgaa tcatttttct caattattgg
     4141 atcagctggt caatcaaacg aagcgaatac ataagatgaa tcctttactt ccgtttgaat
     4201 tatctttatc agaaaaagca atgcatctgg accgttcaat tagcgagttg aatatctggc
     4261 aggcgactgg cgccacagtt atcgcaattt ctcataatga agaactgctt gtttcacctg
     4321 gcccctatgc taaatttacg gcaggggaca tcgttcattt tattggaaat gaatatacac
     4381 tacagcgtat gacaaacttt ttttacccgg agcatcagct tgcagaatga ttgtgtgaaa
     4441 tattaaaaaa atattaactt ggctaaacct tttatttaca aagatttagc caagtttttt
     4501 tcagtttgga caagcaaatt tctttcattt ttgacaaagt gactacatga tgttaacata
     4561 gtgtggtcac ttttttcaga aagaaaaatg aatttacata aaaaaggaga atttgtttgg
     4621 taaaagtaga agtaaaacat ttaacaaaga tatttggtaa aaagacacaa gccgctttag
     4681 atatgatgaa tgatcaccag ccgaaaacgg agattttaaa gaaaaccggt gcaacggttg
     4741 gtgtgtatga cgtgaatttt gacgtaaaag aaggagaaat cttcgtgatc atggggcttt
     4801 ccgggagtgg gaaatccaca ttgatccgtc tattgaatcg tctgattgag ccaacttcgg
     4861 gcagtatcta tattgatgga gaagatgtat cgaagttaag caaagaagaa ttgcgagaag
     4921 tccgtcgtca taaaatcaat atggttttcc aaaacttcgg tctgtttcca catcgaacga
     4981 tcttggaaaa tacagaatac gggttagaag tccgtggtgt acctaaagaa gaacgacaag
     5041 aaaaagcaga aaaagcatta gaaaattcta gtttgctttc atttaaagac caatatccta
     5101 gtcagttatc tggtgggatg caacaacggg ttggcctagc cagagcttta gcaaatgatc
     5161 ctgagatctt gctgatggac gaagcgttct cggctcttga tccgctgatc cggcgagaaa
     5221 tgcaagacga attactggac ctgcaagcca atgttcaaaa aacgattatt ttcattaccc
     5281 atgatttgaa tgaagcttta cggattgggg acagaatcgc cttgatgaaa gacggcgaaa
     5341 tcatgcagat cggaacaggt gaagaaatat tgaccaatcc agcaaatgat tatgtacgtg
     5401 aatttgtgga agaagtcgat cgttccaaag tgcttacggc tcaaaacatc atggtacctg
     5461 cattgacgac gaatatcgaa tctgatggac caaacgttgc attgacaagg atgcgtaatg
     5521 aagaagtcag catgctgatg gctgttgaca gaaagagaca cctgaaaggg atcatcactg
     5581 cagatcaagc gttagaagct aggaagcaaa aacgtccatt gatcgacttc ctagatgaaa
     5641 atgtgacggt gattgggaaa gacatggtgg taagtgatat tttcaatatt atttatgact
     5701 caccgactcc acttgcagtt gtagaggatg gaaaattaaa aggtgtcgtc atccgaggta
     5761 gtgtgattga agcattagca gaaacaagcg aggtgagtga acatgaataa tctattgaac
     5821 tatcaattac cagtggcaaa ctgggtagaa aatatcacag aatggtttac gacaactttt
     5881 tctggcctat tttcattttt acaaacgatt ggacaggcag tgatgtcggg gattacaaat
     5941 ttactgttag tgattccagc tcctttgttt atcttgttat tgacggtagc agctttcttt
     6001 atttctaaga aacgtccagg acttacgttg ttcacattaa tcggtctatg gtttatttat
     6061 aaccaaggtc tatggaatga tttgatgaat actgtcacac ttgttctttt atctagtgtg
     6121 atctcgatca tcattggggt accattaggg atcttgatgg caaaaagcag taaagcccaa
     6181 agtattatca aaccgatcct tgactttatg cagacaatgc caggattcgt ttatttgatt
     6241 ccagcagttg catttttcgg tatcgggatg gttccagggg tatttgcctc agtcatcttt
     6301 gctctgccac caaccgttcg ttttacgaat ttagggattc gacaagttcc taaagaatta
     6361 gtagaagctt ctgattcatt cgggagcaca agtcgtcaaa aactattcaa actagaattg
     6421 cctttagcta aaagtacgat catggcaggg atcaatcaaa caacaatgct gtcactttca
     6481 atggtcgtta ttgcctctat gatcggtgca cccggcttag gacgcggcgt attatcagca
     6541 ttgcaaagag cacaagtcgg gaatggattt gtcaacgggg ttgcattagt tatcttagca
     6601 atcatcattg accgttttac acagcatttg aaccagccaa acaataaaaa agctgcagga
     6661 gcaacaacac agaaaagtaa aaaacgtcaa ggactgatca ttggtgctgt agtagtagtg
     6721 atcttaggtg cgattggtat agggagtttt tcatctgcca aagaaacaaa acgtatcaac
     6781 ttatcttatg ttgaatggga tacagaagtt gcttcaacaa atgtagtcgg tgaagtattg
     6841 aaacaaatgg gctatgatgt aacgatgacg ccattagata attcaatcat gtggaaatct
     6901 gtttcaaatg gcgagtcaga tgccatggtt tcagcttggc tacctaaaac tcatggttca
     6961 caatatgcac aatataaaga tcaagtcgaa gacctaggtg ccaatttgac aggagcaaaa
     7021 gttggtttag cagtgccagc ctacatggat gtaaattcga tcgatgagtt aaccgatcag
     7081 gcaggtaaga aaatcatagg aatcgagcca ggagctggtg tagttacggc tgctgaaaac
     7141 acgatccaaa aatatgacaa cttgaaagac tggaaagtag agacatcttc ttcaggagca
     7201 atgaccgttg cactaggtca agcgatcaaa aaacacgaac caattgtagt tacaggatgg
     7261 acacctcatt ggatgtttgc caaatatgat ctgaaatact tagaagatcc tgaaaacgga
     7321 atgggaagcg aagagcagat ccatacgatg gttcgaaaag gattgaaaga agaccagcca
     7381 gaagcttaca aagtattaga caatttccat tggtcagaaa aagatatgga aaaagttatg
     7441 ttggaaatca acaatggaaa agatccacag caagcagcaa aagactggat caaagagaac
     7501 caagaattag tcgaaagctg gaaaaaataa aaagacagac acgaaataaa tggagcttcc
     7561 aaaattggat caaatatcca aatttggaag ctccatgttc gtctctatct tttttgtcta
     7621 taaaaaaaga gaatgcgtag ctcatcttcg aaacgttcaa agacgccgac aaactattat
     7681 gaatttatgg gtgcgagtca aagagatcga tcgtaggtat gaaagactaa tgtgattaaa
     7741 gtttattatg tacgaagtgc attctcaaaa aatattaaag aatatacata gtttatatac
     7801 ttaaaagtga ttaaattctt
//
LOCUS       DAYYNG010000136         7615 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000136 DAYYNG010000000
VERSION     DAYYNG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.137"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHBFA_002337"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHBFA_002337"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9395766.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACHBFA_002338"
     CDS             complement(2032..3285)
                     /locus_tag="ACHBFA_002338"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9395767.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACHBFA_002339"
     CDS             complement(3291..4214)
                     /locus_tag="ACHBFA_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9395768.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACHBFA_002340"
     CDS             complement(4435..6927)
                     /locus_tag="ACHBFA_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9395769.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACHBFA_002341"
     CDS             complement(6934..7401)
                     /locus_tag="ACHBFA_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9395770.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="ACHBFA_002342"
     tRNA            complement(7537..7610)
                     /locus_tag="ACHBFA_002342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
BASE COUNT         2096 a         1661 c         1226 g         2632 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atatt
//
LOCUS       DAYYNG010000137         7589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000137 DAYYNG010000000
VERSION     DAYYNG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.138"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            432..3239
                     /locus_tag="ACHBFA_002343"
     CDS             432..3239
                     /locus_tag="ACHBFA_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9395771.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3430..3921
                     /locus_tag="ACHBFA_002344"
     CDS             3430..3921
                     /locus_tag="ACHBFA_002344"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9395772.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4024..5013
                     /locus_tag="ACHBFA_002345"
     CDS             4024..5013
                     /locus_tag="ACHBFA_002345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9395773.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5046..5849
                     /locus_tag="ACHBFA_002346"
     CDS             5046..5849
                     /locus_tag="ACHBFA_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9395774.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5870..6784
                     /locus_tag="ACHBFA_002347"
     CDS             5870..6784
                     /locus_tag="ACHBFA_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9395775.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6867..7241
                     /locus_tag="ACHBFA_002348"
     CDS             6867..7241
                     /locus_tag="ACHBFA_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9395776.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7380..>7589
                     /locus_tag="ACHBFA_002349"
     CDS             7380..>7589
                     /locus_tag="ACHBFA_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9395777.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILV"
BASE COUNT         2461 a         1255 c         1674 g         2199 t
ORIGIN      
        1 gaatcttatg attaaagagg ttgtgacaag acttttgtca cagccttttt tgtttataaa
       61 aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt
      121 tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt
      181 gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata
      241 aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta
      301 tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt
      361 tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg
      421 accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc
      481 aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta
      541 aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac
      601 gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt
      661 acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct
      721 ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg
      781 cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa
      841 agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg
      901 aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga
      961 gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga
     1021 ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg
     1081 aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa
     1141 caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt
     1201 cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg
     1261 aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa
     1321 accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg
     1381 tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat
     1441 ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga
     1501 ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa
     1561 tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact
     1621 cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt
     1681 cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc
     1741 tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat
     1801 ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt
     1861 taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt
     1921 tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt
     1981 ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt
     2041 ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac
     2101 gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag
     2161 caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag
     2221 acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag
     2281 aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta
     2341 attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt
     2401 ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc
     2461 atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg
     2521 aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta
     2581 cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat
     2641 tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag
     2701 aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc
     2761 ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat
     2821 cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg
     2881 ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt
     2941 ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca
     3001 aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat
     3061 tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg
     3121 cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt
     3181 attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa
     3241 tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt
     3301 aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct
     3361 aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga
     3421 gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc
     3481 gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc
     3541 gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca
     3601 aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca
     3661 aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta
     3721 cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc
     3781 aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa
     3841 gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta
     3901 gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt
     3961 aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata
     4021 aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc
     4081 ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa
     4141 ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac
     4201 gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta
     4261 ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt
     4321 gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt
     4381 gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg
     4441 aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga
     4501 gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt
     4561 gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta
     4621 tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca
     4681 aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc
     4741 aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta
     4801 gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt
     4861 aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt
     4921 gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg
     4981 aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc
     5041 ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg
     5101 aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt
     5161 tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt
     5221 taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat
     5281 cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga
     5341 ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg
     5401 ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa
     5461 tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt
     5521 tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa
     5581 cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca
     5641 acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa
     5701 tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact
     5761 tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc
     5821 ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga
     5881 aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca
     5941 aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc
     6001 aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga
     6061 attctttaat acacacccat acattgcttc tccaatttta ggggttacat tggctctgga
     6121 agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg
     6181 tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat
     6241 gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt
     6301 cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta
     6361 caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa
     6421 aggtgcttca attttaggga tgttcgtatt agctgccctt gtacaaagat gggtatcaat
     6481 caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga
     6541 taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt
     6601 agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg
     6661 tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc
     6721 aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt
     6781 ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt
     6841 cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg
     6901 taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtgata
     6961 atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag
     7021 aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca
     7081 ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc
     7141 gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg
     7201 tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta
     7261 agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat
     7321 tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta
     7381 tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca
     7441 ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa
     7501 gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc
     7561 catctatttt aggcgcagtg attttggta
//
LOCUS       DAYYNG010000138         7266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000138 DAYYNG010000000
VERSION     DAYYNG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.139"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACHBFA_002350"
     CDS             complement(146..1111)
                     /locus_tag="ACHBFA_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9395778.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACHBFA_002351"
     CDS             1345..2190
                     /locus_tag="ACHBFA_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9395779.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACHBFA_002352"
     CDS             2381..3568
                     /locus_tag="ACHBFA_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9395780.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACHBFA_002353"
     CDS             3574..3930
                     /locus_tag="ACHBFA_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9395781.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACHBFA_002354"
     CDS             3932..4270
                     /locus_tag="ACHBFA_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM9395782.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACHBFA_002355"
     CDS             4665..5699
                     /locus_tag="ACHBFA_002355"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9395783.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACHBFA_002356"
     CDS             5692..6378
                     /locus_tag="ACHBFA_002356"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9395784.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACHBFA_002357"
     CDS             6402..7250
                     /locus_tag="ACHBFA_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9395785.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2406 a         1299 c         1590 g         1971 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaa
//
LOCUS       DAYYNG010000139         7128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000139 DAYYNG010000000
VERSION     DAYYNG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.140"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            243..1403
                     /locus_tag="ACHBFA_002358"
     CDS             243..1403
                     /locus_tag="ACHBFA_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9395786.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLEDHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            1438..2205
                     /locus_tag="ACHBFA_002359"
     CDS             1438..2205
                     /locus_tag="ACHBFA_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9395787.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            2395..2793
                     /locus_tag="ACHBFA_002360"
     CDS             2395..2793
                     /locus_tag="ACHBFA_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM9395788.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3099..3473)
                     /locus_tag="ACHBFA_002361"
     CDS             complement(3099..3473)
                     /locus_tag="ACHBFA_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395789.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3495..3845)
                     /locus_tag="ACHBFA_002362"
     CDS             complement(3495..3845)
                     /locus_tag="ACHBFA_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395790.1"
                     /translation="MKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(3862..4257)
                     /locus_tag="ACHBFA_002363"
     CDS             complement(3862..4257)
                     /locus_tag="ACHBFA_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM9395791.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            complement(4324..4509)
                     /locus_tag="ACHBFA_002364"
     CDS             complement(4324..4509)
                     /locus_tag="ACHBFA_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFM9395792.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4591..4845)
                     /locus_tag="ACHBFA_002365"
     CDS             complement(4591..4845)
                     /locus_tag="ACHBFA_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395793.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(5119..5403)
                     /locus_tag="ACHBFA_002366"
     CDS             complement(5119..5403)
                     /locus_tag="ACHBFA_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395794.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(5897..6382)
                     /locus_tag="ACHBFA_002367"
     CDS             complement(5897..6382)
                     /locus_tag="ACHBFA_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395795.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKQQLDRIKEE
                     NNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(6549..6815)
                     /locus_tag="ACHBFA_002368"
     CDS             complement(6549..6815)
                     /locus_tag="ACHBFA_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9395796.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(6817..7038)
                     /gene="relB"
                     /locus_tag="ACHBFA_002369"
     CDS             complement(6817..7038)
                     /gene="relB"
                     /locus_tag="ACHBFA_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9395797.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
BASE COUNT         2343 a         1152 c         1191 g         2442 t
ORIGIN      
        1 tcgtatttta ttgaaaagga tttttatttt gaaaatgttc taaaatagcg atttctgctg
       61 tattttcaat gaaatttgat tggaaaaaat aaaatgtgtt ttactgacat tgagttatgt
      121 tataaattta cgatacattt cttattgtaa cgtatcgaaa acaggtctat acttattcaa
      181 tggaatcgta ccagttccaa gtttttaagc tcgtaccaag cttagaaaga agaggcaatc
      241 gtatgaaaat aacagaaaag aatgtaaacc cgaaatttga aactaccgaa agcaatctaa
      301 aaaagtttga tttaactgtt actttatcga atcaacttgt agatagcatt gttgaaatgg
      361 atgctatgcc acaaaaaatt tatctttacg ctataggaca tcttgaccct aaaaatccgc
      421 cttttaataa taccgtcaaa gtatctaaaa aggctttatt tgcgttgatg tcttctagtt
      481 catcttcttc aaaacattca agattaaaac aagttattga aaaattgcat cagcaatctg
      541 tcattaaaat tcaaagaact aaacctaacg gtaagactga atacaaagtc atctcaccgc
      601 tagaaattac tgaattcaat gatttttctg atgcagtatc tattaaattt agtagttcaa
      661 ttatgccttt attaacgaac ttagaagatc attttacgca atatacattt gatcaagtag
      721 ttaacttgac aagcaagtat gcgatcagtt tttttcaatt attcactaaa gattttaatc
      781 tgtatgaagc aaatagagat aacttaacat ggagtacaaa atcacttgat agattaaaaa
      841 atcctgtttt ctccattgca gatttacgaa agaaatttag tattgagaaa aaatataaag
      901 cttttcgaga ctttgaaaga cgtgtaatta aagaaccgat tgcccaactg aataaataca
      961 ctgattttaa aattgactat gagcttatca agaagggtca tacttataca aacataaagt
     1021 tcttgcttac acgtaccatg tcagttccgg taccttataa gcaagaacaa agagaccctg
     1081 cttatttaaa aggcgcagaa gataaaacag agaaaatgaa tcaatcgtac ttgaaagcta
     1141 tgaatagcaa atacactaaa ttactaatca aaaacttttt gatcagttct gccgacttat
     1201 gcgatcttaa acttatgagc cgacttcaga ataaagttta tccgctttat gatcgtttat
     1261 ccaaagatag aggatttgaa tcagttgtca atcacctatc ttacgttgct actcataagg
     1321 aggattacac aataaaaaat actgttaaat atctcgaaaa atcaattacg agttatttgc
     1381 cttatgtgaa cctttatgat taacaactga aaaaatgcgt gaaggaagga aaaacttttg
     1441 aaaaaagatg aactgacaaa gtacaatgag gagttcttag caacaattga tatgaaaatg
     1501 gatttaaagt cgtatgaact ttatcaattg ctgttcagtt atatctcacc cacaaagcta
     1561 aagaaaacag gcgtagtaga tttacctgtt aaagcgatta ttaattacat ggatgttgat
     1621 caaaatcttg aaacggaact aaaaaccata gttaacactt tgcaaagaca agcagtttat
     1681 gaaatatcga atctagacaa gaaatataag acgtatacta ctgtatctgc tattgatcgg
     1741 attcattggg atttagaaga gcatagtgta tctttcagtt tttcagaaga tgtaattccc
     1801 ttaattgtct caatcaaaaa gaagtatccg ttctttgttt ttaaagactt agcatctttt
     1861 caaaaaaaat atagcatcgt gttatacaaa tatttggcga tgtattataa aaaatatctt
     1921 atcgccaaaa ataaaggaga ttttgagaaa gctgaaatat ataagaatcc cctgattgaa
     1981 atagttaaaa ttcgtgagat tacgaaaaca gaaaatgagt ataaaacaat atcgaatttt
     2041 gatagacgtg ttttaaggga atcagttgaa gaaattaatg aaaaatcaat gctgaagatt
     2101 gattttgcta aagttagaaa aggcagaaat tacaattttg ctgtccagtt taatatttct
     2161 agtgagtttg atgaaaaacc ttatagagaa aaaaaattag gttaatgcaa aatatatctt
     2221 actcgtttga agatgcttca gacgtatgtt aagatatgta gtgccttggg agcaaggtat
     2281 taatctcctt tagaggttaa ttagcttgtg atcgcctatc aattttttgg taggcgattc
     2341 tttttttgaa accaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa
     2401 tacttttttt gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat
     2461 tttccagact tatatacgca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa
     2521 gtattggaag gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg
     2581 cctgttcaag agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta
     2641 aaagctaata cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg
     2701 actgtggaac actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct
     2761 ttgaatgatt tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa
     2821 aaacagcttg ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa
     2881 aagctctcag aagcgttctg agagctttta aattgctatg tattatactg catctgctca
     2941 aattgatttt cttttcatgc ttaccatgta tctagttttc catcaagtaa tttttagata
     3001 tgtattgagg aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat
     3061 gtgttaggat aaattataca agaaaatgtc aaaagctctc agaacgcttc tgagagcttt
     3121 ttatcggtag gcttgtttaa cgtgatcttg aaagataccc ttttgatgtt tctctcgatc
     3181 cctgcatcgc taattactaa gtgttcttca agtgtgatgt aggtatataa attaagcatc
     3241 aaaatggtgt agataagcaa aaaactccct aaaactaagt gttgattata aaaatctgac
     3301 ttataaaaaa gaactgagaa tgttagtgtt gcttcccaaa aaagaaacaa aattttgtat
     3361 ttccaaagat agtggatcaa gcgctgtgct ttactcatct caaataattt ataataacct
     3421 gctaattgat cagcaagcgt atcgttaatg ggttctgttt gtgtttgttt catctttctc
     3481 ttctcctgtt tcaattaatc taaaatttca gattgagcaa gcaactcatt gtggtagtcg
     3541 gtttgaaatt caaataacaa tttagccttc ttaacgaggt ttaaagcgtt cgttagtgat
     3601 tcacttaatg cttcaacatc tttaactgat tttgcatgtt tcattttttc agtgtgcgta
     3661 tagcagaatt caggtattga gttaggagtt gcctgttcaa taaacatctc aattcgcaag
     3721 ttattatcct caaaaataat tagatcattg gcaaagtgtt tggtactaac gctaatgcca
     3781 gttgtatctt ttaatagatt attgattaac gttcgagaga aatagtttaa gtgtgtagat
     3841 ttcataggtt cggtctcctt tttacttttc aagcaagtct tcttctagtg ctttttgaag
     3901 taattgagag aagtttatgt ttttctcctt ccctaaaaga actaaccatt ccggaacact
     3961 aacatttttt ctaattgttt ttgtatttaa gcttcttcgg aaaagtttaa tgtttagttg
     4021 gatgattact actacttgat tggctttcaa agttaattct tccactgctg atggtttagg
     4081 aaattctgca tctggtacac tatatagcgc taatcctaaa gcatccttag cgtttaaata
     4141 tgcttcttca agactatttc caaaggtaga tatttccggt aagtcaggga aaacgatgtt
     4201 ataagcttct ccttctttct cgaagattgc cggataaaaa atattttttg tcttcatttc
     4261 ctcgctcctt ttctagtgta agagaggata gtatgcctaa taccaaatag gaaggcaagg
     4321 ctgttaaagc cctgcttgct tccagatatt agcttctgtt cctagtggta catcaccatt
     4381 gtggatcgga acaacgaccg ttcttttagt cttggaatta taaaattttg catgactgcc
     4441 agtttgtcca gtctttttga agccgttaga tttgagaatt tttacaattt ctcttgctgt
     4501 cttaggcata gctaccctcc ctttacaaaa tctattatac acattgttgt gtatggaatc
     4561 aatagaaaaa gaggatgttt tattgcaaaa ctaataaaat gatttattgg ttttttcagc
     4621 aaattgaatg ttattagcgt tgcaaatatt cataatttcc caagtatcat tagaagtata
     4681 ggtatctaag aattcattga cagttacggt tgattcgtat ggttcataag tcaaagcgtt
     4741 ctccaagtct tttgatgaaa tgatcaagac tttttcaatt tcctcccctt tttcatttgt
     4801 gaaaacgtat tccaaagtga tttgttcttc atcggtataa ttcatattgt gttctccttt
     4861 cttgtattaa gaaaaatgga tatttccaat tgcatcccac tcggcaccat tgtggtataa
     4921 gacgtgaccg agcgttacgc ctttgaaatg ctcttctaaa tacatctaaa cgcattcaag
     4981 gctagtacct gctttgagat tgtagaagtc tgcatccaat accttttcag aagttatcgg
     5041 atcaatgcga gttggtacct caataaattc agtccaaatt tctgcaaaag tcttaccttc
     5101 caaagtccac actccctatc aattaattcc aaaagcaaat agagaaatca agcgaccgtc
     5161 aatagtcact tcaacaactg aaaatgccgt tgaaacagtt ttaacatcca cactttccaa
     5221 tttccctatg aaggaatttt tgtgctttag aatagctttc tttaactgtc tgttatacgc
     5281 ttgacgactg ttaaactttt tgaattctcg atccttgatc agctgtttaa cgacccctgc
     5341 attaattgta tagctttctg caatagcaac gtgagtaggt tgctgtaatg tagctgttag
     5401 catattgtca ctccttccta aaagttaatt agcttgttta caattacatt atacacacta
     5461 gtgtgtataa tgtaaagaga ggttttttgc tgatggatca gctgtgctaa acaaaagaaa
     5521 acgatagagt aacaaaaaaa gtcagcaaag ttaaaaagga ggaaacggct agtccggttg
     5581 ctttgcttgt gtaagtttcg gctttcttat agatcaaata ggaaattatc agtgatattc
     5641 cagtaaagaa taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc
     5701 cttctgtaat attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcaatg
     5761 gatgttttag ccctacccta aaatcaaagc ccagaaaagc atttaaaaac gttcgtacct
     5821 aatacgttta tattcatata catatacata aacataaaca taaacgtata cacgtttatg
     5881 tttatgtatt taagttttag gctacttcct taacatctcc tgctgatagt cgagtgaacc
     5941 gagtgagtga tccatcttgt agtctaaaaa taaagctacg tttgagttta tactgataaa
     6001 cagcgacata cgatccatcc cagattgagt aagttttacc tgcaactaat aagtcatcaa
     6061 ggtttggttt attgttttct tctttaattc tatctagttg ttgcttaacc attttttctt
     6121 gatcaccgac aaagaatttc aaccattttt tcggaatttt cttaaagtaa gcaggactgt
     6181 catttaagaa gtagattggt ttgtacattt catctcgaaa gatgaagctg tcaatatcgt
     6241 aggttccttc tgcatctttg aaaaggataa taccataggt cttcgttact ttggtatcta
     6301 cgatttccaa accatttttg gcgtaccctt tcttttcttc gatcacttca tttgctgatg
     6361 aataagcacc ataccatccc ataaaaaatc gctccttttg aaagttaatt agcttgttta
     6421 cacttatatt atacacacca gtgtgtataa taacaagagg gtattgttct tttttaggta
     6481 aagtgggatc gtaaacagtg attttgttgg gttaacaaaa aaacagacaa gccttatcag
     6541 cttgtctgtt acttataaac ttcacttctg tgaccaatag acaatataag aattgtcact
     6601 tcatgctcgc taatatcagc tattatacgg taggcaccta ccctatatcg ccagtagcct
     6661 gatttattag ctgttagacc tttaccatgt tttcttggat catcagtgcc tacaagattt
     6721 ttctcgatcc agtttaggat aacctttgcc tgaaacttat ccattttttt tagcgctttt
     6781 tgagcttcac gttcatatct tacttggtaa gtcatcttat aagcctaact cttttagcat
     6841 atcttcgtgg gaaatacttt gatcgttttc ctcatgctct ttcattagtt ccttgtaggt
     6901 ggcaaagtct actaacgttt ctgctgtttg cagggaatta ctacgcataa attcagaaat
     6961 gttatctccg ttgaattcag caactttttc aagaaaatct ttttcttctt tagatacacg
     7021 aatacttatt actggcatag tttatcaact ccttttcaat tacattgtac tacgattgta
     7081 tgacaaatgc aatagttata tatatgaata tatacataaa cataaaca
//
LOCUS       DAYYNG010000140         7071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000140 DAYYNG010000000
VERSION     DAYYNG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.141"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1437
                     /gene="istA"
                     /locus_tag="ACHBFA_002370"
     CDS             211..1437
                     /gene="istA"
                     /locus_tag="ACHBFA_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM9395798.1"
                     /translation="MRKDIYEGVLPFIMTHTKPNYAALAKQYNCDYRTVKRYYEAGIS
                     NKLPLLAQKNKNKKTLVSDFEDIIADKLALGCSSSAIYHFISKRGYRGSARTLRRYCR
                     NIRADKIQKATIRVETTPGLSAQVDWKEDLKLVNKFGEVISCNIFLYVLGYSRIKYLE
                     LTFDRSQTTLFRCLTNAFQYTGGVPTEIWFDNMKTVVDRSRSQFSKVVFNERFRQYAK
                     EAGFLPIACRAFRPQTKGKVEALARTIERIRVFNYEFESTHELEKIIQTIMDSLNTEE
                     ISQAIHECPSDRWSYEKEQLSSFDANQLKIYSSVEKQIKRKVSNEAMVQWNQNKYSVP
                     VNYIGKILTLRVKEDKLYIYDDSTLIRTHTITHQLLNYHRNDYIEILRSDVFKHLEDE
                     ELERFVDENLQAYDEL"
     gene            1450..2205
                     /gene="istB"
                     /locus_tag="ACHBFA_002371"
     CDS             1450..2205
                     /gene="istB"
                     /locus_tag="ACHBFA_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM9395799.1"
                     /translation="MSNYYQLLNKLEDLNLKHTREILPEYLDTIVQENISFVEALNHL
                     MTEEINARSERQKENRLRKANLPFKKTINDFDFGFQPKINRTEILDLCTLRFMDTNNN
                     ILFIGNSGVGKTHLAIAITLEAITKGYSCHFALSNELVNRLNRANQRGNLDRLLKKYA
                     SYDLLIIDEIGYLPFTPDGAKLFFQLINLHYEKKSTIITTNIPLSQWAETFQDKKLTN
                     ALIDRLVHHAKIIQITGKSYRIKDYKEKKLSYK"
     gene            complement(2256..4022)
                     /locus_tag="ACHBFA_002372"
     CDS             complement(2256..4022)
                     /locus_tag="ACHBFA_002372"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395800.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGKVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(3982..5736)
                     /locus_tag="ACHBFA_002373"
     CDS             complement(3982..5736)
                     /locus_tag="ACHBFA_002373"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395801.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(5846..6415)
                     /locus_tag="ACHBFA_002374"
     CDS             complement(5846..6415)
                     /locus_tag="ACHBFA_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9395802.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(6433..>7071)
                     /locus_tag="ACHBFA_002375"
     CDS             complement(6433..>7071)
                     /locus_tag="ACHBFA_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9395803.1"
                     /translation="KVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKI
                     IRPTKGKIIENKKISSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMEL
                     YKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKE
                     KGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         2415 a         1262 c         1017 g         2377 t
ORIGIN      
        1 gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa ttataactat
       61 atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgtaaat ggccaagtta
      121 aaatgtagaa aaatggccaa ataaaaatgt aggtttatgc gtcatctggt acgctttcct
      181 tgtgttattt tattcaagga ggaaagcgta ttgagaaaag atatttatga aggagtgtta
      241 cctttcatta tgactcatac aaaacctaat tatgctgctc tagctaaaca atacaattgc
      301 gattatcgta cagtaaaacg ttattatgaa gctggaataa gcaataaatt gcctttactt
      361 gctcaaaaga ataaaaacaa aaaaactttg gttagtgatt ttgaagatat tatcgctgac
      421 aaattagctt taggctgttc ttcttctgcc atttatcatt ttatttctaa acgtggctat
      481 cgtggaagtg cacgaacact aagaagatat tgtcgaaata tccgagcaga taaaatacaa
      541 aaagcaacga ttcgtgtgga aacaacccct ggtttatctg ctcaagttga ttggaaagaa
      601 gatctaaaat tagtcaataa attcggcgaa gttatttctt gtaatatctt tctttacgtt
      661 ttaggatatt ccagaattaa gtacttagaa ctaacttttg atcgttctca aactactcta
      721 tttcgttgct taactaatgc ctttcaatac accggaggtg ttcctacgga aatatggttt
      781 gataatatga aaactgttgt tgaccgtagt cgaagtcaat tttctaaagt tgtttttaac
      841 gagcgatttc gacaatatgc gaaagaagcg ggatttcttc caattgcttg tcgtgctttt
      901 cggcctcaaa caaaaggtaa agttgaagct cttgcacgaa caatcgaacg aattcgtgta
      961 tttaattatg aatttgaatc cactcatgaa ttagagaaaa ttattcaaac aatcatggat
     1021 tcattaaata ctgaagaaat ttctcaagcg attcatgaat gtcctagtga ccgttggtca
     1081 tatgaaaaag aacaactatc ttcttttgat gcgaatcaac ttaaaatcta ttcttctgtt
     1141 gaaaaacaaa ttaaacgaaa agtatctaac gaagcgatgg ttcaatggaa tcaaaataaa
     1201 tattctgtgc cagtaaatta catcggtaaa attcttacac ttcgtgtaaa agaagataaa
     1261 ctttatatct acgatgactc cactcttatt cgcacacata caattactca tcaattgctt
     1321 aattatcatc gaaacgacta tatagagatt ctacgttctg atgtgtttaa acatttagaa
     1381 gacgaagaat tagaacgctt tgttgatgaa aatttacaag cgtatgacga attatagagg
     1441 tgaatcatta tgtctaatta ctatcaatta ctaaacaaat tagaagattt aaatttaaaa
     1501 catacgagag aaattttacc agaatatctc gataccattg ttcaagaaaa tattagtttt
     1561 gtagaagcgt taaatcattt aatgacggaa gaaataaacg cacgaagtga acgccaaaaa
     1621 gaaaatcgtt taagaaaggc gaatttaccg tttaaaaaaa caatcaatga ctttgatttt
     1681 ggatttcaac caaagattaa tcgaacagag attttagatt tgtgtacctt acgctttatg
     1741 gatacaaata ataatatatt gtttattgga aatagcggag tggggaaaac tcacttagct
     1801 attgccatta cactagaagc tataaccaaa ggttattctt gccattttgc tttaagcaat
     1861 gaattagtca atcgactaaa tcgtgcaaat caacgtggaa atttagatcg attgttaaaa
     1921 aaatatgctt cttacgattt actaattatt gatgaaattg gttatttgcc ttttacccct
     1981 gacggggcaa agttgttctt tcaattaatt aatctacact atgaaaaaaa atcaaccatt
     2041 attacaacga atattccttt gtctcaatgg gctgaaactt ttcaagataa aaaattaaca
     2101 aatgcactaa tcgatcgatt agtgcaccac gctaagatta ttcaaattac tggaaagtct
     2161 tatcgaatta aagattataa agaaaaaaag ttgtcgtata agtaaccttg ttatttatgg
     2221 gccaaaaacc tacattttaa cttggccatt gacaatcatc gtgattgagc ttctttttca
     2281 tagacgaatt tctcagatag tttagttttc tttatgagat ttttatatat ttctgacctt
     2341 cgatacaact cactatgagt accttgatca actacttttc cttgatccat cactacaatt
     2401 ttatttacct tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt
     2461 attaaacgat ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta
     2521 atttcatcta aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt
     2581 tgtcgctctc ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca
     2641 ggtagttttt caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca
     2701 tctgacgctt gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt
     2761 acctcttgaa aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata
     2821 tcatacccat caattaaaat acagccttca tcataatcat acagtctaga cactaatttc
     2881 aagatactag ttttcccgga accactagca cctactaacg cagtcacttt tccttgttta
     2941 gccgtaaaag agatatgatc taaaattggg gtattgttat catatgagaa agagacatct
     3001 cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc
     3061 ttcatagctc taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct
     3121 ttcatagaat cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag
     3181 agtatagtca cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa
     3241 ctaacatatg agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt
     3301 aaatgaatcc tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat
     3361 agattaaagc tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttt
     3421 tctctcaaag tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag
     3481 attaagacaa aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca
     3541 accaaaagca tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt
     3601 gcatgactca tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct
     3661 gataaattat gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc
     3721 actcttaaat gcgcactttc tttataagta ctattatata agttttcata ctccctatct
     3781 aacaaccaat acatgaaaag caaagtaaca aacgatacgg caaaataaag ccaacggggc
     3841 tttgtatttt ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata
     3901 ttaatgcagt atacaaaaaa actagaaata gaagctttag ttaaagcttc tgcgccctga
     3961 tcacttaaag caaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc
     4021 attctcataa ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg
     4081 atttccacgt tctactattt tcccttctga caagacaaga atttcatcta cagatttaat
     4141 gctggataat cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc
     4201 cttttgtagt tcgtactcat tatctggatc aatagatgca ctggcttcat ccataataac
     4261 aatctttgca tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga
     4321 cagataaact cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat
     4381 cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt
     4441 tgctatagaa acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa
     4501 agcaattgtg ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt
     4561 aatctgtccc ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc
     4621 gcttcctgat tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt
     4681 ttctaaaaca cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt
     4741 tttaatctgt gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga
     4801 gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct
     4861 catgaaagaa acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa
     4921 tatcttggga gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac
     4981 tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt
     5041 atatgcaaac ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt
     5101 agcaccaaat atctttataa cttgaattcc gcggatatat tctaccgttt cagagcttaa
     5161 cacatctaac gattcttgat atttttgaat aaaatttgta ttctctgtca ttttctttaa
     5221 caagaataca ccaattacta acatggcgat taaaaccaat ccaattttaa agttaatgaa
     5281 aaagcctaag gcaactgata aaacaggtgt tactatggct tgagcactat ctggaatcaa
     5341 gtgagcaatt gccatatgtg ttttagccgc gttgtcatca attgtctttc gaatatatcc
     5401 cgaagaattt aaatcaaaaa aacgaaaact tgcttctgtt aaaccgctaa ttcccttttt
     5461 tctcaaatta gtctctaagc gaaacccaag gatatgagaa aataagccag aaaaaatata
     5521 aaataaagct cctaaagtta accaagcaac agtttgaacc gcataactct tagctaaata
     5581 ttcatctcct acaataatta gagactgaag aaatttgtaa atactgtaat atccatagac
     5641 tgttaatacg acagaaaaat cagaaaataa agtggctaac catccgtagt atttcatttt
     5701 agggacataa ctaaataatt ttttgtatac tttcattttt tcctcccccg cattcaattt
     5761 cttctcttat tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt aaagatgtac
     5821 aaacagatag aaggtaaact acgttctatt ttttgtattt ttttaagact tttcttccaa
     5881 tcaatacacc aatcatagat aaacttgcta caactaacac tgaaatgatt ccccatatac
     5941 ctaccgcata gtggatcatt gcatccattg ttgcagcaga ttgtccttta tctatccaat
     6001 cttgacgaaa acgatctgta aagaaccata taggaatcca ctgtccggca gcactgccta
     6061 attgcattag agcccaagac gcaacttgag gaattgctcg atcatatcca aatacagata
     6121 gaataatatc agctaagatc gccatactaa tcgaactgat aaaccaaggt aagtacgccc
     6181 cgcccattaa cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa attccttttc
     6241 tagggtagtt atagctaata taaacaaata tgattccaga taataatcca aaaatccctg
     6301 gggatatgct gtgaccaaac gccccaccaa tactactaac cattattgaa aaaaagtaaa
     6361 ttactaggga aataactgta gctaaaccaa taaaaatata atcttttgtt ttcatatata
     6421 ttctcctcaa ttctatttcg ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc
     6481 tactctaagt tttatatctt tttttattcc accatttttt aaataaataa ttctattgca
     6541 tgttttataa agaaattccg gatcatgagt ggctataact actgcaccct tttcttttaa
     6601 gtctttaata aggtcagcaa tcctttccat attaatataa tccaacccac ttgtaggttc
     6661 atcaaataga aataaattag tttttgacaa tgaagctata gaaaccaata atctttgttt
     6721 ttctccaccg gataaatcaa acggtaattg atctttcatt ttataaagtt ccattttttt
     6781 taaaattttt tcttgctgat ctttactaac tatttcattc cccagggcta actcgctcct
     6841 cactgattct gtgaaaaatt ggtaatccat ttcttgcatt actaaaaacg gcgatgaaat
     6901 ttttttattt tcgattatct ttcctttggt agggcgaata atcttcatga gtattcttag
     6961 taaggttgtt ttccccacac cgtttttccc taccaaacca ataacatctc caactttgac
     7021 atcaaaattt atgtctttta gaatattttc ataagtaatt tcttcaactt t
//
LOCUS       DAYYNG010000141         7033 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000141 DAYYNG010000000
VERSION     DAYYNG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.142"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..777)
                     /locus_tag="ACHBFA_002376"
     CDS             complement(<1..777)
                     /locus_tag="ACHBFA_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9395804.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSG"
     gene            complement(789..1742)
                     /locus_tag="ACHBFA_002377"
     CDS             complement(789..1742)
                     /locus_tag="ACHBFA_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395805.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(1732..2802)
                     /locus_tag="ACHBFA_002378"
     CDS             complement(1732..2802)
                     /locus_tag="ACHBFA_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395806.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(2808..3851)
                     /locus_tag="ACHBFA_002379"
     CDS             complement(2808..3851)
                     /locus_tag="ACHBFA_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395807.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(3852..4793)
                     /gene="opp3b"
                     /locus_tag="ACHBFA_002380"
     CDS             complement(3852..4793)
                     /gene="opp3b"
                     /locus_tag="ACHBFA_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9395808.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4877..5224
                     /locus_tag="ACHBFA_002381"
     CDS             4877..5224
                     /locus_tag="ACHBFA_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM9395809.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(5294..6955)
                     /locus_tag="ACHBFA_002382"
     CDS             complement(5294..6955)
                     /locus_tag="ACHBFA_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9395810.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         1945 a         1476 c         1300 g         2312 t
ORIGIN      
        1 cccagataat tgttcaagat aatcttctcg ctggttcttt tgtttcagat aaagatccat
       61 tacttttctc ttttgtttct gataattgct tgcctgatct aacctgatct ttctatggtg
      121 agcggagcgg actttcacat tcaatttccg ctgtgcatgt tgcagctttt cttctgtctg
      181 gtgaaatttt ggtaaagtta cttctagagg tcgagatgtt tggatcaatg tcttcggatg
      241 ataggcgatc ccgatccatt tagctaaaga ttcttttttt actggcgaaa catcacaaag
      301 tagtgcgaca taaaattctt cattatatcg agcagaaatc gtagcggagc gaatcgatcc
      361 ttcaactggc cgatgctgat ttatcttgat tagttctttt tgttttggta actttaagta
      421 gccattttcc agatagatcg tacgggtttg gttgttggtc gtataagatt gccacatact
      481 ttttttcgtt ttaagctttg ggaagctggc acgtcccttg aaataattgc gaaaagcccg
      541 atccaaatta agctgtgcat ttgctaaagc aagactgtct acttctttta agaatgggta
      601 ttcttttttt aatgtagcgg gtgtgagcga aaaattggct actcctgttt gttgccaact
      661 tttctgccgc tttttcagca aatggttata ggtaaatcga gcacatccaa aagtacgaat
      721 caattgctgt ttttgttctt ctgtcggata catacggaat ttaaaagctt ttagcatcta
      781 tgtccctcct agttagcaac cagaagagat tccttttgtc gttttagttt ttttgcgtaa
      841 tatgggactt cctgctcagt tgcatagaca aaatgttctg gtgcgatttc acgaagttga
      901 cgtacttgtc cgtcatctgg ttcaggttga tatttgattc ttctgcgttt tctctcatgg
      961 tctggatctg gtagaggaat agcagataac aaactttctg tataaggatg aacaccgtga
     1021 ttatagattt catcacttgt tcccatctct aataacaagc cgttatgcat tacaccgatt
     1081 cggtcactaa tatgtttaac catcgacaaa tcatgtgcaa taaatagata ggtcaattga
     1141 tgttcttttt ggagatcttg caacaaattg accacttggg cttggatcga cacatctagt
     1201 gcagaaattg gttcatcaca aatgataaaa cgcggtttta ctgccaatgc tcgtgcaata
     1261 ccaatacgct ggcgttgccc acctgaaaat tcatgaggat aacgagtacc gtggcttgga
     1321 ttcaatccaa cagttctcaa tagatcatcg actttttttg cacgctcttc agatgattta
     1381 gctagtccat taacatcaat tccttcggca atgatatctc tgactttcat acgaggattc
     1441 aacgaagcgt atggatcttg aaagatcatc tgaacatcat gccggaattt cgtcaaacct
     1501 tttttatctt tgatcttcgt tatatcttga ccgtcgaata atatctcacc atcagtaggt
     1561 tcattcaagc gaatgatcgt acggccagta gtggattttc cgcttccaga ttctccaact
     1621 aaaccgaatg tttctccttc ataaatgtga aagctgatgt catttacagc atgtacttca
     1681 ttcttttttc ctacattgaa atactgtttc aatcctttaa cttcaagtag tttatttggc
     1741 atcagcaatt cctccttgac cgtaagtatg tttttgtttc tctttaaaga tagcccagcg
     1801 acgttggatt tcttctggcg gtgtcacttt gggtgcctgc ggagcaagta gccaagttgc
     1861 tgccttgtgt gtttttgata gttcaaaaaa tggtggttct tgttctgcat cgattttcat
     1921 cgcgaattca tttcttggat agaatgcgtc tccttttggc gggttcaata aatccggtgg
     1981 agaaccagga atagcataca atcgatcgtt cgcactatct aatgtcggca ttgaaccaag
     2041 cagtccccaa gtatagggat gttgcgggtt gtagaaaatt tcttccgatg ttccaacttc
     2101 aacgattctt cctccataca tgacagcgac acgatcagcg acattcgcta ctacaccaag
     2161 gtcatgagtg ataaagataa ttgatgtatc gatttttgtc tgaatttctt tcagcagctc
     2221 caaaatctgt gcttgaatag tcacatcaag cgctgttgta ggttcatcag caatcaggat
     2281 ttccggataa cataccagtg caatcgcgat cacgatccgt tgacgctgac ctcctgagaa
     2341 ctgatgcgga taactgttga tacgtttctc agcttcgggt attccaacta atttaagcaa
     2401 ttctaatgca gctttttgtc cctctttttt tgaaacttta ttatgctttc taagggactc
     2461 agcgacttgc ttcccaatgg gcatcgtcgg atctaaagag gtcattggat cttggaaaat
     2521 catcgctatt tctttaccac ggattttttg catctctctt tctgattttc caacgatatc
     2581 gttaccttta aagaggattt gaccagcatc tatattggcg ttgcttgaca gcaagcgcat
     2641 gatgcttctg gtcgtcactg attttccact tccagattcc ccaacgatcg ccaatgtttc
     2701 tcctttattc aaatgaaaat tgacaccgcg aatcgctcgt acctttccag catacgtatc
     2761 aaaagagatt tctaagtctt tcacttctaa aattttcgtc atcgtcttca ctctttcatt
     2821 ttaggatcaa aggcatcacg caatccatca gcaagtaagt taaagccaat catgataacc
     2881 gataaagtta ctgccggaat ccataataga taaggcagat acaggaatgt cttgtaccct
     2941 tcgctcaaca tcgttcctaa tgaagctgac ggaggcgtaa gacctaaacc gataaagctt
     3001 aaaaaggctt cgaagaaaat agcagatgga atactaaaca tcatttgaat aataatgaca
     3061 ctagagatat tcggcaaaat atgtttgaat gcaattttca ttcttgattc acctaatgtc
     3121 aatccagcta aaacatattc ttgatctttt aatttcattg tttgtgcacg aacgatacgt
     3181 gccattggta tccagtttgt gatggccata gcagccacga ttgaaaaaat tcctggttca
     3241 aaaacgacca acatcaaaat catgacaacg aggtttggaa ttcctgataa gatttccaaa
     3301 aaacgttgca tcacgttatc gacacgtccg cctaacaaac cagaaaccaa cccgtaagct
     3361 acaccaatcg ttacatctag taatgcagca ataaatgcga tcagcaacga gattctcatt
     3421 cccataaata agcggcttaa tacatcacgt cccaaaccgt ctgttcctaa aaagaaattt
     3481 acgtttgaag ggacgtttgc ttgagcatat ttatcaacaa gttcaccacc aaccattgct
     3541 tttccattca atccatcgat attgattccc ggaatacgag gaggcaaatt gatataagcc
     3601 acgttttggg cagttggatc atgaggagaa acaaagattg tgatcacaga gatcaaaatg
     3661 atgaccaata gtaaaaccat tgaaataacg gcagctttgt ttttctttaa acggcgccat
     3721 gaatcttgga taaaattcaa agaaggtgca gtaatctgtt cacgttcttc ttcggtattt
     3781 ttttgtaatg gaagaaattc atccgcaggg atatctgcaa ctgtttcata acgctttggt
     3841 atcatttcca tttatcctcg gctcccttct gaaacacgaa ttcttggatc aactagaccg
     3901 tacaatagat ccacgatcaa aataacaaaa atcagcataa atgagtaaag gatcgttaca
     3961 gccatgatcg taggataatc atttgttgtg attgacttaa cgaattgttc cccgatacca
     4021 ggaatagcaa agatattttc taccaccagt gatccggtca tcaatgctac agctaacggt
     4081 cctaataaag tcatcaatgg aatcaaacta tttcgaagac catgtttaaa ggcgatttcc
     4141 caacggctca atccttttgc ttttgctaat tccacataat cactatgcag tacttccacc
     4201 atttccgtac ggataaaccg agcagagtca gcaagcggcg acatggccaa cgcaattgtt
     4261 ggtaagattg tgtacacaaa gccatcccat tttgcgattg gcaaaacttg taatttgatt
     4321 gcaaagatat attgtaataa aacagcaaat acaaagtttg gaatggaacg acctaatatc
     4381 gctacgagtg tagaagatgt atctgcccat gtattttgct tcatggcaga gatcgttcct
     4441 aaaatcgtcc ctaggactgt tccaaagata attgcctgta accctaactg caaggatggt
     4501 ccgattcttc cagctaacaa atgtgcgact ggctggtttt taaattggaa ggaaattcca
     4561 aaatctcctt gaagcaaatt agaaagataa atcccatatt gtacgataac tggtttatcc
     4621 aaaccgactt gtttatttag catctcgatt gtttcaggac tcaaacgttc ctgattcgta
     4681 tatggcgtgc ccggcagcaa ttgcatcaag aaaaaggtga ttgttgcaat taaccaaagc
     4741 gtgatgatca tgaaaaagac acgttttaat acatatttga tataactatt cactaatgtt
     4801 tccccctagc ttttgacttc tgacagatgt ttcgctacat tattagataa tgaaccaaaa
     4861 ataaaggagc aatcaaatga aaaaaaaatc tatcccctac gcagtagctt ttctactgat
     4921 tctggttatt ttaataaaaa atctcatcaa ccattccttt acactcattc aattatctaa
     4981 tgatctattc ttatggtcat tgccgtttct gattatcggc ggttttttat gggtcttttc
     5041 ttcgggtttc ttcgatcatt ttcagcgatc ggtccatctt gcacgaacaa ggaaccgaaa
     5101 gaaaaaacca gaattttcct cgctctcgtc tgccagctac ggtatgtaca gcttttggct
     5161 gatcattgcc ggaatactaa ttgctctttc tgctattttc atgctttttt ctttgttagg
     5221 ataagtgaat atgagaacga ggttgcgaca tttgccgcag cctcgttttt tctcatgctc
     5281 acggtattaa cgattattct tcaacgtatg tccatttgta atcccatgaa gcgcctgccg
     5341 catggtgaac gatacctttg attttttcat tcactaggtg accttctgct ttttggtaca
     5401 ccggaacgac acccatatca tcagagatga tcttgattgc tttttggaaa tcatcccaac
     5461 gggcttgttc atcgccagcg tctgttgatg aagctgcttt aactgcttcg tcatattctg
     5521 cattgctcca gcgaccacgg ttgtatgaat tacctgtgac aaacaaatca gtaaagctgc
     5581 ttgggtctgc atagtcagca gcccatccgc ctagtactgc gtcaaaatca cccgaagttg
     5641 agcggtcaag acgtacagag aatggaacag gtgttggttt gactgttacg ccatccaagt
     5701 tttcttggat tgagttttga acatattcga ttactttttt cgatgaatca tcatcagaag
     5761 ccatcaaatc aaattctaaa gaatcaacgc ccaattcttt tttcgctttt tcccaatatt
     5821 cttttgcttt atctttgtca taagaaacta attttccagc ttcttttgca aagtcttcgt
     5881 tcgttgttgg actttttacc atatctgatg ggatcaatcc tgtagagaca actgatccgt
     5941 cacctagtac ttgttttact aaagcttcac gatcgataga ataagaaata gcttttctta
     6001 ggttcacatt gttgaatggt gaattctttt cacgttgatt gaattcgata tagcttgtcc
     6061 gcgcttcttt aacagaagta taagctggat cgtttgcgtt ttgctgtgct aactcaccag
     6121 acaagatcac gtcatctgct tggccatctt tgaataagtt caatgcagta gaagattctt
     6181 ttactacact gactttgatt tcagaaagtt ttactgtatc tttgtcccag taatgatcat
     6241 ttttcttata agtccattct gtatctgttc ctggtccatc aaaatcttct aatgtgaatg
     6301 gtccattata aacagcttta tcacttgcag atgcataggc atccccattt tcttccacta
     6361 cggattgttt ttgtgggaaa aatgatggga aagcaagcag atattggaaa tatggtgttt
     6421 gtttcgtcaa tttgatttct aattcatagt caccaactgc tttgatccct agttctgatt
     6481 tatctttctt tccagctgta atgtcttctg cattttcaac tggcgcatac aggtaagcat
     6541 attcagaagc tgtagctggg tctactgttc gttgccagcc ataaacgtaa tccgctgctg
     6601 taacaggatc accatttgac cattttgcgt cttctcgtaa tttgattttg taagttaatc
     6661 cgtcttcact gacttctgcc aactcgctag ctcctgccgg ctgtggtttg ctgtcttcat
     6721 caagacggta aattccttca taaacgttat tcaatgctgt aaaactgatt gtatctgttg
     6781 caactgataa gtcagctgta ggcatttctt gaggcacgac taagttaaat acgcctcctc
     6841 catctgctga accgcttttt gctgaactac tgcttgtggc tgaactgccg ccataacatc
     6901 cagcaagtac aaggccgcat accgcaacca aaccaaatgt aaatttcttt ttcattttac
     6961 gtaccccttt attggaattt tcagaataat taatattttc tgaagttgtt taagagttta
     7021 atgaataatt att
//
LOCUS       DAYYNG010000142         6915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000142 DAYYNG010000000
VERSION     DAYYNG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.143"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            349..2391
                     /locus_tag="ACHBFA_002383"
     CDS             349..2391
                     /locus_tag="ACHBFA_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395811.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2402..3022
                     /locus_tag="ACHBFA_002384"
     CDS             2402..3022
                     /locus_tag="ACHBFA_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9395812.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3034..3492
                     /locus_tag="ACHBFA_002385"
     CDS             3034..3492
                     /locus_tag="ACHBFA_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395813.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3511..3789
                     /locus_tag="ACHBFA_002386"
     CDS             3511..3789
                     /locus_tag="ACHBFA_002386"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395814.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3814..5181
                     /locus_tag="ACHBFA_002387"
     CDS             3814..5181
                     /locus_tag="ACHBFA_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9395815.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5196..6341
                     /gene="dgoD"
                     /locus_tag="ACHBFA_002388"
     CDS             5196..6341
                     /gene="dgoD"
                     /locus_tag="ACHBFA_002388"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFM9395816.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6368..6859
                     /locus_tag="ACHBFA_002389"
     CDS             6368..6859
                     /locus_tag="ACHBFA_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395817.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
BASE COUNT         2176 a         1135 c         1500 g         2104 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaaaagcat tgtttttagc agtttaatat tattcttttt
       61 caattagact gacttgattt agttacggaa tagatacttt aatgtgttga agaagcaacg
      121 tcccgtgttt atttactaaa gcatccaata ttctaccgaa tatttaatat tcacattagc
      181 cctaaaataa ttgtcatacc atgttacatt tgatataaca gcgttcattt taaaaatagc
      241 tgttagtgtg acaaaacatt aaaccaagtg tgacaaaaaa cgtgatgaaa gctttccgga
      301 aacgtatgtg aacaagtata taatgatttt gtcaggagga tagtcaaaat gaataaacga
      361 attcgtgaaa tactcaaact gattattaaa aaccctgaaa tgaaactttc cactttaaca
      421 agtgaacttg atttgacacg tcgtcaaatc aattacgcga tcaatcaatt taatgaagaa
      481 ttgaaaacaa agaacattcc gttaattcag agaagtcatt ctggaaatat tacggtacca
      541 atcgaggtta tccaaatgat atcacagcct gattacgaca taggtgatga tcaggaactt
      601 gtcgttctca ctgatagtga aagacaagca ttgatagtga tgactctgat cgctaatatc
      661 gattacatca gtctggatca tttggtagat gcggttgatg ttagtaagac gactgttatg
      721 gaagatatca aacgaacgga accgttcatc agaaattata gtctgacaat tcgatataat
      781 cggatgaatg gttaccaatt ggttggttcg gaacaccgta tcttacagct gttatcggat
      841 ctagtaaaac ggtttccgat ttttcgccga gaaatgattc gcagaaaatt ggcacaagat
      901 gtttctgaag aagaaatcgt ccatttgatc cataacatgg aacaaatgtt gcatcttagt
      961 tattctgatg agtcggtaga ctatttgcag acatcagcga gatttttagt aagccgtggg
     1021 atcaagatgt cagataagca cgcgtttttt cccgatgatg tgacggatac tcctgaattc
     1081 cgaatgctga tgatcttggt acatgaaaca ggttggagtc tatccaaaag ttatatggag
     1141 tggttcacgt tattattttt aacttccaat atttttgaga gcaaaactac tcaaaattat
     1201 gattcggatc aagaattgcg aaaattgatc agccaaatgg ttgaaaattt ccaaaatcaa
     1261 acgttgattt tgatcgatga ccgagaagac tttgaacgta ggatactgag tcatctgcgt
     1321 ccagcctgtt ttcgaatacg ctataacttg agtttaggag tttattcaat agatagtctg
     1381 atcaaggata gtaatcatgc gattttgatt gatttgatga aagaattgat tattcctatt
     1441 gagaattggc taaataaggc ctttccaaat gatgaattag atcttttatc ctattacttt
     1501 ggctatcaat taagtaatca taataaattg aataagcaac gacaaagggc tgtagttgtt
     1561 tgtactaatg gtgtcatggt ctcaaagtta gtacgagcca atatggaaaa actttttcca
     1621 gagatccatt ttttagcttc cctatctgtg cgtgatttct acaaatttga agctgattat
     1681 gatctggtct ttactacgac gccactaaaa agttctatgc tgcagtttat gatcgagcca
     1741 atcatgacat atcagcaaca aatcagttta cgatatcgag ttttgagtga tttaggaatt
     1801 actaaggttg atcgagcaat ggatgaattg ttaacaatca ttaggaaata tgcaaatatc
     1861 accaatccag tttcgttgcg agaagaactg cagtactttt tgatcaagga agatggaagc
     1921 acgccgctgg acaacttcaa tgtattgcct tcattgactt attacttgcg gccgagttat
     1981 gtacaaatcg ttgaagaaga gatgtcttgg gaacgagcag tggaggtggc ttgccgaccg
     2041 ttattggaac atcaaatcat tgactcgcgt tttatcgctg attgcttgga tcaaatcaga
     2101 caaccgggat atgcaggtta cttgggaatg aagacttgca ttccccatac aacggtcgat
     2161 aaaggtgtaa tcagtgacgg tgtcagcatt ttagtttgta aaaagccgat acgatttcct
     2221 aatggtgaag atgtcagctt gattttgccg ctttcatttt ttgatttaac gaagcatctg
     2281 cgagccatca atcaaatcgc tgatatcgcc aaagatgatt cattgattga gcagttattg
     2341 attgctgatg acgaaaagac agtttatcaa ctattaagac aaagatctta aggaggaaaa
     2401 catgagacta gcagattatc ctaaattgac agtgatcatg cgtggttata cttttgatca
     2461 ggcagatgct attttacaag caatgcagga atttgaccat caatttgccg tagagatgac
     2521 aatgaacaca aaaggagctc ttgaaaatat acatgaacta aatcaacgtt atggtgacac
     2581 gacttatatc ggcgctggga cggtaaggac attagaacaa gcccaagcct gctatgaggc
     2641 tggtgcaaaa tttttattag gaccacatat gttcacaaaa gaaatgttgt cttacgccaa
     2701 taagaaagga ttattggcgg tacctgccgc aatgactcct tcagaagtcg accgtatgtt
     2761 taaagaagga gctgatattg taaaagtatt tcctgcagcg gttgttactc cacgtttttt
     2821 caaggatatt caagcacctt taggtaagtt accactaatg ggggttggtg gaatcagtaa
     2881 ggagaatgcg aaagagtttt ttgaacaagg tgctagctat ttaggtcttg gttccggtat
     2941 gttcaacaag caagacatcg aagagctaaa tgttaaaaac ttagcacgtt cgatgaaaga
     3001 attaattgag gctattaaat aggagtgagg actttggaac tttatttgaa tgaggaactc
     3061 gtttttcgaa atgttcaagc aaaaacagat agtgaagtct tagcgttttt agcaagcgag
     3121 atgtacaaaa aaaactacgt gaaggaggaa tatattcaag caatccaaga acgggaaaaa
     3181 gaatacccta ctggattacc atcaacacca ccaggaatcg caattcccca tgcaaactat
     3241 gaaatggtca ataaaacaac tttagcaatt gcgactctta aagatccggt cctatttcat
     3301 aatatggaag ataacaatga gcagttacca atccaaattg tgatcatgat ggcgattggg
     3361 gaaccacatg ggcaagttga aatgttacaa aaaattgttg gaatcataca agatgagcca
     3421 ctgcggcaag aaatgatcag agcaggcaat gatactgaat tattagaact attaaagaaa
     3481 gcggtttttt aatataagga ggaaatcata atgaaacgaa ttttagtagc atgcggaaat
     3541 ggtatcgcca cttcaacagt agtagcaaca aaagttagag agtatttgca acaacatggc
     3601 attgatgttt caacgactca aacaaaattg atggaagttc caggaaaagt tcaagactat
     3661 gatctattgg tgacgactgg tcaatttgat ggtcaaacag gtggtgtacc agtaatcaaa
     3721 gggatgccga ttttaacagg tatcggtgcc gatcagacaa tggaagagat tttgaactta
     3781 ctgaaataat agctgatcaa aggagctgaa aaaatgaaaa tcattatgaa cgtctttaac
     3841 ttctttattg atgctggacc aacagttatg ttgccagtca tcatcacgat tattggtttg
     3901 atttttgggt taaagatttc tcgtgctttt aaatcaggtt tgacattagg tatcggtttt
     3961 gccgggatca agttgatatt ggactttatg acgaccaatg ttggaccagc tgccaaggcg
     4021 atggttgatc gaactggtgt taaactagat gctttggatg ttggctgggg ttcgattgcg
     4081 gctgttactt gggcatcgcc aatcattcca atcttgattt ttgccattct attggtcaat
     4141 atcgttttgc tgattttgaa acgtacacat acgctggatg ttgatatctg gaactatcac
     4201 catatggcaa tcgttggtgt tatagtgtat tttgtgacta agaatgtctt cttaggtgta
     4261 ggtgcatcag tagtgatggc aattgcgact tttaagattt cagactggtc gcaaccgatg
     4321 gtagaaagtt tcttcggaat ccctggtgtt tctttaccaa ctgtttctgc gctttcatca
     4381 ctggttattg cttggccgtt aaattggctg ctggatcgta tcccgttatt cagaaaatca
     4441 aaattcacca tcaaagatgc acaaaaatat ttgggatttt ttggtgattc tatgatcatg
     4501 gggttagtta ttgggatggt gattggtgtt ttagccggct atgatatcaa agcggtttta
     4561 caattaggtg tcagcatgtc agctgttttg gttttgattc ctaagatgac ttcattattt
     4621 atggaaggat tgatgccaat ttcagatgct gcacaaaaat ggtcacaaaa aaaattcaaa
     4681 ggtacacgtt tatttatcgg attggatgct gcagttgttg taggtaatcc agatgttatc
     4741 acaacagcat tgatcattat tccactaacg attgcattag ctttagtatt gccaggaaac
     4801 cgtgtgttac cattcgcaga tttagcggtt gttcctttcc gcgtggcaat ggtcgttgcc
     4861 ttgactcgtg gtaatttatt aaaaaatatc attattggct tagttgtgac agcttcattg
     4921 ctatggtgtg ggtcagctac tgcgccaatg ctgacagcaa ttgccaaatc ggttggtatt
     4981 aacttaggaa cttcttcaat gttaatctca tcatttgctg cgacttccat gatccaaagt
     5041 tatttggtat ttatcgcatt tgcttacaaa ccaatcatcg gtattccagt gttgttatta
     5101 gcctttgcgg gcgcatggta ttacttcgat ggtatcaaaa aagttaatca tgaaatggat
     5161 gaaccattga aggcacaata gtaggagggt ttaaattgaa aatttcagat gtcacagtgt
     5221 ataaagtaaa accaagatgg atttttgtta agatctcgac tgacgaagga atcgaaggct
     5281 ggggagaaat gatctcagga actaaaacag aaacggttgt tgctggagct tatgagatag
     5341 gaaaccggct gattggacgt aatccgtttg aaatcgagcg cctcttccaa gaaatgcatc
     5401 gatctttctt ccgaggcggt cctattaatg gaaccatcgt ttcgggttta gaaatggctc
     5461 tgtgggatat caaaggaaaa gcgtttaatg ttccggttta tgaattattg ggcggtgcag
     5521 cacgtgataa aatcaaggtt tattcttgga ttggtggaga tcgaccaagt gaagttgcag
     5581 aacaagcaca agatcggttt gatcggggct ttacggccgt caagatgaac gcgacagaag
     5641 agctgcatta tatcgattcc taccaaaaag ttgatgaagt ggtggaacga gttgcgtcta
     5701 ttcgcgaacg ttttggtgat gcaatggata ttggtgttga ttttcatgga cgggtccata
     5761 aaccgatggc aaaagtgcta gcaaaagctt tggagccatt ccatccaatg tttttggaag
     5821 aagtcgtttt acctgaaaat gaagaacact ttaaagaagt agctgattct gtggctgtac
     5881 cattagctac tggtgaacgc ttgtacactc gttggcaatt caagaacctc tttaaacaag
     5941 gtactgttga tattatccaa ccagatgtgg ctctttgtgg gggaatcttg gaaacccgta
     6001 aaatcgcagc gatggcggaa gcttacgaca tggctgttgc gcctcatgcg ccatatggtc
     6061 ctgttgcact agcagcaact ctgcaagtag actcatgtac gccaaacgtc tttatccaag
     6121 aacaaagctt agggatccac tataacaaag gttttgatct gttagatttt gtgaagaaca
     6181 aagaagtttt ccaatataaa gacggctacg tagatctacc ttcaaaacca ggtctgggct
     6241 tggaaatgga tgaagacaaa atcaaagaaa ttgcacaaga aggtttgatt tggaccaatc
     6301 cgcaatggaa aaattatgac ggtacgatcg ctgagtggtg ataaatgaaa ataaaggatg
     6361 atgaaagatg gcagaagcac ttatgatcac agttttttta ggactgctga ttattttgca
     6421 aataatcgat caaaagaaaa ttatcgtgtt tgctcagagt agtcctgtaa ggaagagttt
     6481 gagtatttta ctgacagtcg ccatcctgtt gattttctgg ccggaacatc tgactgatca
     6541 gattaaactg gttgcttttg cttctgtcat tttgatcaat ggttttttga aggagggtct
     6601 agcaaaagat tctgttgtta gatttggagt attgaacggt aactttaatc gatatcctca
     6661 agtccaagta gaaaaactag gtgacgataa atcatttatt tcattttaca aaagcaagaa
     6721 taattctttt tcaatgattt ttgataaaga tcaaactgaa atgatggatg tctttcaaga
     6781 acttgatatc cattccaaaa ttactttaga tcaaagttcg gaagaaaaaa gagcagttgt
     6841 aaaaaaagat aatgaatgat tctactaccg aaattatgat ttctttatca cgaataatga
     6901 aacttgtttt aatga
//
LOCUS       DAYYNG010000143         6885 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000143 DAYYNG010000000
VERSION     DAYYNG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.144"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHBFA_002390"
     CDS             complement(<1..182)
                     /locus_tag="ACHBFA_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9395818.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHBFA_002391"
     CDS             517..951
                     /locus_tag="ACHBFA_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9395819.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHBFA_002392"
     CDS             974..1294
                     /locus_tag="ACHBFA_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395820.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHBFA_002393"
     CDS             1435..1626
                     /locus_tag="ACHBFA_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395821.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHBFA_002394"
     CDS             complement(1697..1954)
                     /locus_tag="ACHBFA_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM9395822.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHBFA_002395"
     CDS             2112..2375
                     /locus_tag="ACHBFA_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395823.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHBFA_002396"
     CDS             2399..2587
                     /locus_tag="ACHBFA_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395824.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHBFA_002397"
     CDS             complement(2670..2855)
                     /locus_tag="ACHBFA_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395825.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHBFA_002398"
     CDS             3070..3441
                     /locus_tag="ACHBFA_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9395826.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHBFA_002399"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHBFA_002399"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHBFA_002400"
     CDS             complement(3709..4137)
                     /locus_tag="ACHBFA_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395827.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHBFA_002401"
     CDS             complement(4197..4955)
                     /locus_tag="ACHBFA_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9395828.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHBFA_002402"
     CDS             5250..5447
                     /locus_tag="ACHBFA_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395829.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHBFA_002403"
     CDS             5553..6035
                     /locus_tag="ACHBFA_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395830.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHBFA_002404"
     CDS             complement(6093..6689)
                     /locus_tag="ACHBFA_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9395831.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1059 c         1353 g         2142 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttt
//
LOCUS       DAYYNG010000144         6861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000144 DAYYNG010000000
VERSION     DAYYNG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.145"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHBFA_002405"
     CDS             33..656
                     /locus_tag="ACHBFA_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395832.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHBFA_002406"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHBFA_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9395833.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHBFA_002407"
     CDS             2152..3033
                     /locus_tag="ACHBFA_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9395834.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHBFA_002408"
     CDS             3036..3806
                     /locus_tag="ACHBFA_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395835.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHBFA_002409"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHBFA_002409"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9395836.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHBFA_002410"
     CDS             5117..6280
                     /locus_tag="ACHBFA_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9395837.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHBFA_002411"
     CDS             6397..6807
                     /locus_tag="ACHBFA_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9395838.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
BASE COUNT         2308 a         1172 c         1499 g         1882 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt t
//
LOCUS       DAYYNG010000145         6853 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000145 DAYYNG010000000
VERSION     DAYYNG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.146"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..553)
                     /locus_tag="ACHBFA_002412"
     CDS             complement(218..553)
                     /locus_tag="ACHBFA_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395839.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(587..781)
                     /locus_tag="ACHBFA_002413"
     CDS             complement(587..781)
                     /locus_tag="ACHBFA_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395840.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFKTIS"
     gene            complement(948..2918)
                     /locus_tag="ACHBFA_002414"
     CDS             complement(948..2918)
                     /locus_tag="ACHBFA_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395841.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2933..3799)
                     /locus_tag="ACHBFA_002415"
     CDS             complement(2933..3799)
                     /locus_tag="ACHBFA_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395842.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3789..4022)
                     /locus_tag="ACHBFA_002416"
     CDS             complement(3789..4022)
                     /locus_tag="ACHBFA_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9395843.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(4034..6190)
                     /locus_tag="ACHBFA_002417"
     CDS             complement(4034..6190)
                     /locus_tag="ACHBFA_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395844.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(6210..6455)
                     /locus_tag="ACHBFA_002418"
     CDS             complement(6210..6455)
                     /locus_tag="ACHBFA_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395845.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6541..>6853)
                     /locus_tag="ACHBFA_002419"
     CDS             complement(6541..>6853)
                     /locus_tag="ACHBFA_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395846.1"
                     /translation="SGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFGDAKWGDGLK
                     YVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLVSKFPKNELL
                     G"
BASE COUNT         1949 a         1334 c          932 g         2638 t
ORIGIN      
        1 actttcaaat taaactaatt agttatattc tcacaatagc atttaataat aaatatactg
       61 tcgcttgatt tatatatttc cctcttcttt ctttagaatc tctctttatt ttgatataat
      121 aaaatagaaa tcgcaccaaa caatgaaatg gcggaaaaca tttcattgaa aaaaggattg
      181 aacataatgt tcaatccttt tttatagtta taatcaatta acattcggtg tgtgaaattg
      241 aatcttcgat gctttcacat ggaaaattat tgattccttc ttcactcatt tcccatgtag
      301 tatagaaagg agttaactgt cctccatact cctgaataat aaaatatttt ccatcattga
      361 tatggttaca acagtacact ccaatagttg gatactcgac tactaaaaat tggtctggat
      421 caaactgttc ttttttcaac atttctcgta tttgaaacaa atgatccttc tctaagcaat
      481 caggtaattc ctttattctt tgcgttacta atgacccctc tatcgctttc cattcattta
      541 aatattgttt cataacaatt cattcctttt atgttaacta tttttcctag gaaattgttt
      601 taaaaaaaac tctcgctttt tcttcatcag taaagttttt atacaactga ttacaataat
      661 gaggtttttg aagtttctga atacctatac taacttcaaa tccttcttca tgttgaatta
      721 ggattacttc tgtgtctcta cctagaaata ttcgatctaa aatttttatg tttggtttca
      781 ttatttaacc tccactataa ttatttcact cagtaaggtt tcactttatg aaatttctat
      841 agttctcttt tcacaaaacg taccatgttc tctaagactg tttttagtct caaatataca
      901 tgttttttct tatcctttcc ttatacactc aaaatcaaag ataaaactta aagaataaac
      961 tctccaataa caaaattatt agtcagatcc actggtctcc tgatctgaaa tttattactt
     1021 cttcctgttg tcaaatactg tctcaaatca acagaaagtt tttgatcatc ttctcctttt
     1081 ctagcaggag caactttttt ttcatacttc attaactctc cactggtcat ctttccaatg
     1141 atagtgtaat taaaatcact ttcaaaactt tggtaaattt ttccatctgt attgaatata
     1201 tcagctgttt caatatcttc tttgtattca ttgtcttctc ctgaaccgat tgtatcaaat
     1261 agaaaggcaa gacgaattcc atcttcttca agcattgtta atcttttttt aattagccca
     1321 acggtttcaa tagataactt atcgaagccg tgaatcataa caatatcatc tttttttgct
     1381 tgtgatgtga tgaaatcaaa ggtatttaaa aattgagctt ccataacatt ggtttctgct
     1441 cttagatcac ctaagttata gtaaaactgt aatttgtcac ttttaccgat ttctttcagt
     1501 gtcgttcttt gattaaacat cctagagaag ctgtctaatg ctgttctcaa tgtgtctttt
     1561 aatacaataa tacgatcgac ttctttttca gtaccagatt ttttggcttt tgtttcagca
     1621 ttagtcaaat agctaataaa atccccaaga actggcattg tatcactact gttcaccaaa
     1681 agttttgaac gttcttcata attaatatcc caaagtttct gacttatata aaagttacct
     1741 aattcttttt tcaataaaac tctagttgca gaatctaatg ttctgcctgc taacaaataa
     1801 aaaatctgag ttagtttatc gagattccgt ctatataaat caccaatgtc cttttgatta
     1861 ccaaaaagct ccaaagagtt aagaccttct tcagccaaat taaccttttc tattacactg
     1921 tttaacgaag gtttggcaac aaatgttcta ccagttggat caccaaagta atcaaattta
     1981 tttaagacaa tatgaaaagt tcgtttatga tttgccattg cattattagc aatcaattgc
     2041 ccccaaagtg atgatgctga gattttttca tcatagcctt taattgtagc gtttttgtgt
     2101 gatgccacta aaactttact tgtaaaagaa tcatccagtg ccataaaagc tacaccactt
     2161 gcgtagctag tagacatagt gccgatagcc catccattcg catcatctaa acctttgcga
     2221 attgcatgat catttcctgc aaaatccgaa ctcatagacg tatactcata gacattcccg
     2281 attggttcac ttaaaagtct atcaaacaga tcacgttgat ctcctccata tgacataagc
     2341 tttaatccat acattttttc atcatacaac cgttggagtt taattagctg ttcgctgatt
     2401 ttattaggag aatcggatac taataggatt tctagcttcc aatcaataat tttatttgcc
     2461 atgctgtcta gtaaagatgc tgtattcaaa tccataatcc gcataacagt tttgctaatt
     2521 tcagcatctg aattttttcc ttctaaatta ttttcataac tattcgttga tcccccaaca
     2581 ttctcgtgca ttatttcttg ttgatctttt tgtgacactg gcttatctgc ttcaattaga
     2641 taggctttta ccccttctac tctcacttca ggaatagctt taaccatcca accaaatcca
     2701 taatttctat tcattccata agtattcact aatttcacaa ttgttcccca ccgcttacct
     2761 gtcctaatgt aagatggtgt aaattgataa ccaactaaag atgttaaatc actaacaaag
     2821 gtattattca ctacctttga atttctagtt ttttgttctt ttttagtttt tctctttttt
     2881 ttgaaattta atagttgtga tctacccata atttccattt aatctctcac ctctatttca
     2941 gtgcataaat atcagataat atttcttttg tttgtttagc tgacaactga gtgacactat
     3001 aataaagtcc tctactcgta tagttttcta aacgttccac accttcatcc aataccatct
     3061 tagattgaga tttcaagata agatattgga ttactgttgt tttcacacca tcaatatatt
     3121 ctgatacaaa tcgttcttgc tgatctatta tgtcaaagac tgctttgtta ctccttgcat
     3181 ttcgtttttg tattttcata tttcgagttt gtctttccgt attctgaggt tgagatgaag
     3241 tgattttgat ttcagctaag gaacgatctc tggtattttg atattgtatt gatttcgatt
     3301 cctgagccat aacttcttct ggaaaagcgg ttagtgatgt agccccatct agattgaaca
     3361 ttaatgctac actcccatct cgatatacga ttgttccatc ttgtttcact tttaaaatct
     3421 ctgtgcctcg aaatggtttc tttttcccca tagatactct tgctgaatta actactgatt
     3481 tgaacagtct aatagaacca gaaatcaatg aaccactaga tggatcaata aacatatact
     3541 ggctaattag aataagaaat ccaattatca ttaataacgt tggaacaaaa ataaatttca
     3601 gtttaatcgt atcagaaact aagtaaacaa ttgatccaag aacaacgact gcaacaacta
     3661 tagctgatac aattaatctt tttgctgtaa cagaataggt gctattgaca tgcttttcaa
     3721 gccattcaac tctgacaatt gtttcagaag gtttttgatg taagttatgt ttaaatccat
     3781 agtttgagtt atctgccatc agaaatactt cctttcacgt tcaacttacg gtaaacacct
     3841 acagttatca ctattgcaac aataagcaag ccaattaaag atattgactt gctcattgtc
     3901 tcgttcgttt gtggtagttc atttacctga tacacgagct gtgcttcctg taagtgctga
     3961 gaagaggtca tgttttcttg attgttttga attactggat aaactatttt ttcttctcgc
     4021 ataatagccc tccttagtta ttatcagata agtgagcttg aattttgtac tgatttccgt
     4081 ctggagtttt cacaactgct ttttctccat caatagaaat cacttcagcc ttagttgttt
     4141 tcccatcaat actgatgaca acatctttcc caactttgaa ctctgtagtt ggtgttgctt
     4201 catagctact atttgttgaa gcagagttcg tatttgtccc aatcgaattt tccactctcg
     4261 aaatagatgt ttgagtttga gcctgtgctt gttgattatt atgactaata aagtaggacg
     4321 atcccaagat cgttccgcaa gccattgcag cgacagaaat gatagtagcc atctttttac
     4381 ttgtaaaatc acgttttgaa cttgattgat tttctgtagc aactgctaat gatttcgttt
     4441 tttctgccat ttgagctaga tcacttcggg aacgagataa gtcttctctt aaagcttcac
     4501 tttgttctgc caacttttcg ctatttaatt tataaccctc tgctaaatct ttgacctctt
     4561 tcattcgatc tttatattct tcgatagttt tttgttgctc tacaagtttt gtttctaatt
     4621 ctttcgtgtt gttagctacc tgcttttcaa cttcttcttt gttcgcaaaa tatttgacaa
     4681 ccacatctgt tgttttctct tttgtatcat ggaactcagc ttcattttct gactttgttt
     4741 tttcgacaat ctctaaagtt ttttcttgca cgtctgttaa tgatttttca atttctggtt
     4801 tcaatgaatt gataattcgt tccaccacaa cttcttttgc ttcttggctt tgtttcaatg
     4861 catcgttttt aatagattca attgcttctt ggtactgttg atcaagactt tcttcagttg
     4921 cttttctata ttcatcttct tgttgtttaa cttctggtaa atgctcagca tcataccgtt
     4981 gctctagttt ctttagttca gcatccaaat aagcctgttt cgccatatca taatctttac
     5041 gaatgatatt ggatttttcc atcactaaat catcaatttt agatgcttct gcttcgtagc
     5101 tctctttagc aaccgatagt gtatttgtaa actgattttg accatttaat gaggttgctt
     5161 cctctatccg caaaagctca tcagctaaca tttgctttcc tttgaggatc aattgctcct
     5221 tcgtcttatt taattcactg ttgattttat tattagcaat agagatgcga gcgttcatac
     5281 tctctttagc taaaaacagc aatggtttca actcaggtat ggatagcatc tcctcgttta
     5341 aatcgacagg gtcattaatc tcatctaaaa ctacaggttg aaatttgtca gcaatgatct
     5401 gttcaaaatt tttttctaaa aaatcagcat ctaagttttt cggtacatca ctttcagatg
     5461 tgattttttc agcttcataa acataatttt ctttgtcact ttgggattcg ggtttttcat
     5521 caatttttgg ttgttggtag gtttctgttt gtatttgtgt ttcttctatg cttacttctt
     5581 ggttttctgc ttcggttggt ttgttatcat ccacaaaaag agcttctttt tcatctggta
     5641 cattttctgc tacggcttca aattcaactt cttcagaaga gttgaaggtc tcagcaattc
     5701 gttctaatcc attagtagct acttctaatt gctctactgc gctatagaag tctgtagctt
     5761 cttcaacttc tgattcaaac acattaggat catccggaat aactaataat tgaccactac
     5821 tatccataaa acggacacct gctaatagct tctctttaaa tttttctggt agatcagcta
     5881 cataattcaa acttgacttc aatagaacga catacttctc agaatcgaga atagcatcat
     5941 cctcatctat aactccctca ctgttgagct tttgtacatc ttcaattacc atataatttt
     6001 tctttggtaa atctttggtc agcaacgcat cgaattcttt ccataccttt gctttagctt
     6061 tttttgcctt cgcttctttt tcttcttttt tgtacacttc gagtaattgt ggcttcaaag
     6121 agagtgcaac tacaggaact gtttctgcca cataattaat agattcggta atatctttga
     6181 attttgccat tattgtaaaa ctcctttttt cagtcaatag ttcttttctg tttatctagc
     6241 atatatacat atgctttttc gaatttaata agcgtttcaa gtctggcttt ttcaatcttt
     6301 ttactcccat ttcgataatc atagaactgg ttaacattca tattcattat ctttgccagt
     6361 tcggtacctt tcagactgct cattaatact tcgttggcgt gagctaacat tttttctcta
     6421 tcaaaaacca tcttacattt actttcttcc ctcacttcta aactacgctt ggagcgtagt
     6481 tttttggtaa aaagtaaatt gaacaaatcg gcatattatc caaatttgtt caattcactt
     6541 ttaacctaac agttcatttt ttggaaattt tgaaactaga tagataccaa caactggaat
     6601 aagtgctgat atgatattag ctttttggtt caaatcatta cctgttttag tacccattgt
     6661 gtaaagcata acggttgtaa ttacaggaat aataaagatt gcaataatcg ccccaacata
     6721 ttttaatcca tctccccatt ttgcatcacc aaaaaatttc attgcagtga atcctggttt
     6781 caacgaaaaa acgaatgcca agaaatacaa aacaacttgt actaaagttt tcatatcagg
     6841 cgtcattccg cta
//
LOCUS       DAYYNG010000146         6769 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000146 DAYYNG010000000
VERSION     DAYYNG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.147"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1614)
                     /locus_tag="ACHBFA_002420"
     CDS             complement(61..1614)
                     /locus_tag="ACHBFA_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395847.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1762..2931
                     /locus_tag="ACHBFA_002421"
     CDS             1762..2931
                     /locus_tag="ACHBFA_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9395848.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3057..4136)
                     /locus_tag="ACHBFA_002422"
     CDS             complement(3057..4136)
                     /locus_tag="ACHBFA_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9395849.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4245..4685)
                     /locus_tag="ACHBFA_002423"
     CDS             complement(4245..4685)
                     /locus_tag="ACHBFA_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9395850.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4742..5152)
                     /locus_tag="ACHBFA_002424"
     CDS             complement(4742..5152)
                     /locus_tag="ACHBFA_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9395851.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5333..5767)
                     /locus_tag="ACHBFA_002425"
     CDS             complement(5333..5767)
                     /locus_tag="ACHBFA_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9395852.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5867..6715)
                     /locus_tag="ACHBFA_002426"
     CDS             complement(5867..6715)
                     /locus_tag="ACHBFA_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9395853.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2403 a         1235 c         1124 g         2007 t
ORIGIN      
        1 tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat cttatttggt
       61 ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt cttttaattc
      121 aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg tgatgattcc
      181 ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaaagggc agtcgctgat
      241 aacgatgttg cagttctcca taaaatcatt ttgtattttg atcgattcat ttgaaaaaat
      301 ttgttctatc ttaacgatca gatctttact gatataaaaa tcacgcgtat agttcacacc
      361 aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa tgtactaggt cttcaaccat
      421 ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt gttctctgaa
      481 atccatataa aattgttcaa tggcggtttg gacttctttc aacacgctat gttcttgata
      541 aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc cattataaag
      601 tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta taaacaaatc
      661 aaatacagta caaaaggact ccaccatttc tcttgaataa agaacatgag gaacgtttgc
      721 tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt tcgtgataga
      781 aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca gattaggtcg
      841 caaaatatct acagagggat caataaaaaa gtcaatatat tcatactctg tgaattcttt
      901 ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta atagaaaata
      961 taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa aaaaagtatt
     1021 catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag tacttgaaaa
     1081 gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc cagaaaaaga
     1141 ctttctgcca ttattagaaa aggagataga caaaccgtac aatgctaaaa atgcattaat
     1201 ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac ttaaactgat
     1261 aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga ctgattcgtt
     1321 gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt caaaattcaa
     1381 ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac gctcaaagtc
     1441 ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga aatgatctgc
     1501 taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa tatctaattt
     1561 tgtattgctt tttttcttca agagaaacgt tttaatcgct tcttgagttt gcatgaaaga
     1621 ggctcctttt tacttaaatt gttaaaactt gaaaatatta ctgttttttt cataaattta
     1681 gacaaaaaaa cagtaaacat ataggtagaa tctctactat cttatttctt gcatacaaaa
     1741 aaatcaagga gtgagagagt tatgagatat tcaaccatct taatatcaac atttgcagcc
     1801 ttaagcttat cgcttactat aaatcagaca gtccacacag aggagataga aacagccaaa
     1861 tggacagaaa ccaagtgggc caacgtcaat cgaattacct ttcaggatga attttcatcc
     1921 ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa agcaaaaatt
     1981 gactattcag gagacttagc acctatacta caatcagctg ttacatttga gaatgttgat
     2041 cccgctttgt cgattggcta tgacgagaac actttgacta tccaacacaa gcaagcgata
     2101 tttaccttca ccgttacatt aaatcaggac ttgttaaaac cagcgctttt tactgtcaag
     2161 gtatcagatg gactaccgaa cgatcatcat catttaacgc cgtatagtca gcggcagaca
     2221 attgagcaag attcagctat cgactcagga ggagatcttg ttgaagaacc aacagataaa
     2281 ccagaaaatg aaaataagcc agaagtaccg cctacagaaa atccagacgg agaacagaaa
     2341 ccggaaatag aacctggaga agaacccgat acagaaactc aaccagagcc agacaatgaa
     2401 tcaaaacctg aaataacccc tggagaaaaa ccagatgtag acccagaaga aaaaccggat
     2461 gtgactccag aaccagatac ggattctgga aaccaaacag taccggaaac aaatccagac
     2521 acagataatg aaactgaaaa tccagaaaaa ctagaggtag atccagaaga aaaaccagat
     2581 gtgactccag aaccagatac ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa
     2641 aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat caaatctagc aattctaaaa
     2701 ataaatgaag agcagctgaa taaaaaatca cgaatatttg atagcgccca atcagctgaa
     2761 accttaaaaa gtagcaagga tacaaccttt gctagtcctg aaacgaaaaa taaacagcta
     2821 ccaaaatccg gagagtcaca aaacaaagtt atactgtgga gcggaatcat tctattaagt
     2881 atagcaacaa tgctttctgc aaaaagattt aaacaaaaca gatctctcta acatttgaat
     2941 aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc agaaaacgac cccaaaatac
     3001 cagattgaaa tctgatattt ggggtcgttc atactttatc tttcttcata tttattttaa
     3061 gggatatctg tttcttttgg aagatggttc gggatctttt tcactttcaa tacatctaaa
     3121 aagaaggtaa agatcggaat gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta
     3181 acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg ttttgctgga catcaacttc
     3241 ggattcagca cataagactc aattagatgg acaatcaaaa tcgtaatcag gatataaaca
     3301 acatctcgga tgcctccttg ggaataagct atgaaagata gaggaataca agaaatgata
     3361 acacctgcta ctgggatcag acttaaaatg aaaatcatga tcgctaaact cagcagctga
     3421 tgaaagccaa atacagccaa ggcgattgta gtgagcaacg tgtttaaaag agcgataaca
     3481 aattgagctt ctaataccaa accaaatgta ttgacaaact tatcggcaaa atagtaaata
     3541 tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc ttgagaatac aattgttttt
     3601 ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca tagcaaacgc cactgccatt
     3661 tttcctatat cctgtaaata gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa
     3721 aaatcgtttc gctcaatata agtatggata tattgtaaaa caggatctgc atcttttggc
     3781 tgatgctcgt aaaaatcaat aacggactgg atcatatcaa atgtttgatg aataagcatc
     3841 gggatatact ttgtgaatgc cagatagata aggtaaacga tcagtgtata aatcatgata
     3901 gctaaaacac ctgtagggat tcggacaaat cgctgaatga tttgaacaag atgaatcatc
     3961 aaataagtaa agataaatgt gagcaaaatg gttgtaatca tacttctaac taagaaaaga
     4021 acaaaaacga caaataataa aacagtgaga cggcgtattt tttcattcgc caaaaaacgc
     4081 tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa taaacacgtt attcattata
     4141 tgatggaata tgaaaataag aaagagaaag agcaaaaaga aaaaccgcta aatgacgtta
     4201 tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc ctttttctgg
     4261 tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc caattcttcc
     4321 tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg tttttgcaac
     4381 gcagcacctt gaggagtcag cgtaacattt acgattcttt catcacccgt ttcacgctga
     4441 cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt accagaatcc
     4501 agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca taaaactaac
     4561 atcacaagat actgggtata ggtaagattc agcggcttga ggagggaagt gtagtgtcta
     4621 atgatttcct tagaggcggc atataaagga aaacatactt gattaactaa atatagatct
     4681 tccatttttc cacctccgct gttttactat agtgtatcat agcggattta cacaaaaaag
     4741 tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct tttgaatacg
     4801 gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa agaccttcga
     4861 tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa taaagggaaa
     4921 cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc aattctaatg
     4981 cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg gttgcatttt
     5041 cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga ttatgaactt
     5101 ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc ataaaattac
     5161 cacctttaat tatattttat acaattgaat tttacacaat ataattgtgg aaagcaaaga
     5221 aatggattgg cataagataa ataagagaaa gcacatgaaa cagacgcgct ttctcgaata
     5281 gcaaacatat acaaaaaaat atcagagaca aaaactaatc tcatcataaa cattacatat
     5341 atttctcaaa aagttgtttt ctttcttcaa aacttacttc acgatggacc tgatgaagca
     5401 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
     5461 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
     5521 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
     5581 aaccaagaga cgtgttttca tccagcatat ggaatctcgt gccatacaag gtaaaaatga
     5641 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
     5701 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatctatt tccgttcctt
     5761 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
     5821 agttaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
     5881 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
     5941 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
     6001 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
     6061 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
     6121 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
     6181 caatagataa aatagcataa agaaactctt ctattccaaa aacaaacaga ttaagagaaa
     6241 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
     6301 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
     6361 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
     6421 ttgtttctgg aactattgca atgattaaag gaaaaaacaa gcttccgaaa agtactttaa
     6481 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
     6541 aaataactat agctcggtca aatcctaaaa cagcttccag taatattcct agaccgctga
     6601 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
     6661 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatc
     6721 cacttcctta attcattaat aatactctta tcttattata acttacttt
//
LOCUS       DAYYNG010000147         6707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000147 DAYYNG010000000
VERSION     DAYYNG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.148"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..313
                     /locus_tag="ACHBFA_002427"
     CDS             164..313
                     /locus_tag="ACHBFA_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395854.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            394..990
                     /locus_tag="ACHBFA_002428"
     CDS             394..990
                     /locus_tag="ACHBFA_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9395855.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1003..1902
                     /locus_tag="ACHBFA_002429"
     CDS             1003..1902
                     /locus_tag="ACHBFA_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9395856.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1905..2390
                     /gene="ssb"
                     /locus_tag="ACHBFA_002430"
                     /pseudo
     CDS             1905..2390
                     /gene="ssb"
                     /locus_tag="ACHBFA_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2435..2668
                     /locus_tag="ACHBFA_002431"
     CDS             2435..2668
                     /locus_tag="ACHBFA_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395857.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2827..2949)
                     /locus_tag="ACHBFA_002432"
     CDS             complement(2827..2949)
                     /locus_tag="ACHBFA_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM9395858.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3104..3363
                     /locus_tag="ACHBFA_002433"
                     /pseudo
     CDS             3104..3363
                     /locus_tag="ACHBFA_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3806..4012
                     /locus_tag="ACHBFA_002434"
     CDS             3806..4012
                     /locus_tag="ACHBFA_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9395859.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4012..4263
                     /locus_tag="ACHBFA_002435"
     CDS             4012..4263
                     /locus_tag="ACHBFA_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395860.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4279..4716
                     /locus_tag="ACHBFA_002436"
                     /pseudo
     CDS             4279..4716
                     /locus_tag="ACHBFA_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4827..5063)
                     /locus_tag="ACHBFA_002437"
     CDS             complement(4827..5063)
                     /locus_tag="ACHBFA_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395861.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIILFKWITF
                     FLLSYRALPVYFAPPKRRGRLERLVRNKNHFLSY"
     gene            5161..5340
                     /locus_tag="ACHBFA_002438"
     CDS             5161..5340
                     /locus_tag="ACHBFA_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395862.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5399..5755
                     /locus_tag="ACHBFA_002439"
     CDS             5399..5755
                     /locus_tag="ACHBFA_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395863.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         2409 a         1071 c         1328 g         1899 t
ORIGIN      
        1 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
       61 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
      121 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
      181 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
      241 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
      301 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
      361 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
      421 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
      481 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
      541 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
      601 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
      661 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
      721 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
      781 cgctatggga atcctattat agcttatgat ccagaaagaa ctttatgtga catttggaat
      841 ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta tatggagtcg
      901 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttaccaac agacaaaacg
      961 atgcgttctt atattatggc tttaaattaa aggagaagat acatgacacc tacgcagtta
     1021 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     1081 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     1141 gtgttaaaag gcggcttctt gattggctca aagtatggca ttgaaaatcg cacaaccaaa
     1201 gatattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattaac aacggttcta
     1261 aacgaaattt tttcaacgcc gacgaaagaa gacattcaat ttgagattca aggaatgaaa
     1321 gaaacgagag aagccgatta ctatccagga tttagcttac gtgtgctggc acatttggaa
     1381 aacatgcggc cagattttaa ggtagatgtg actacaggtg actcgatcta tcctgcaacg
     1441 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     1501 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     1561 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     1621 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     1681 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     1741 aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga gacaatggat
     1801 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct
     1861 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     1921 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     1981 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag
     2041 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     2101 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     2161 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaagta
     2221 aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccgcca
     2281 cctttgaaca acggaataca gcgacgccta acaacaatgg attgaacgct tcacagaatc
     2341 catttggtgg acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag
     2401 aaggagtaag catggcaaaa aaattagtct gacgatggat ctattgatac ccgtaattgc
     2461 atttatagac gggatttctt tgggcgtcac tgaaaatcct ttggtaaata gaattgcgaa
     2521 aggatcacta tttcttgtat tcgtgtatct tgtctgttct tatcttttaa aaagagcagg
     2581 aaaggataat tccttttggc gattgactaa agtcacagta gcagcgacag ctgttatact
     2641 tacggctatt ctaccgcttt atatgtagag ttaacgggaa atcgttgaac ttcttgcgaa
     2701 atattgctat aatgaacatg aaaaagagga gttgcgctaa caacccctca ttaacagcac
     2761 cgattaaaga acggtggcag aatattagtt atttggaaat aaccgctgac ctgctaaagt
     2821 agacggttat ttctttttgt catttttaag catatttaca atcaaactaa tcaaagcaat
     2881 ggtaaacata ccaaaaccaa gaatggtttg aattgtctca tatgcggaca aaaggcttac
     2941 tcctttcata gattttagca cttacattca taagcaccac cacctttcca gaagtcgcca
     3001 ccgtcttcaa actttactgt cttgactatt atagcatatc taaaatcgaa taaaaattct
     3061 caattttagc tgtttttgct ataattttag cgaggtgatc gtgatggaac aatatattgg
     3121 tttactcgat aaattagggt gatcaagaca cagccattgc ttgtgcgatt tacattacgc
     3181 acgagtcatg aaccaatcaa ttgtgtagtg gccaatatag aaatagcgga taaattgctg
     3241 attttggaag aaggcaagta tactatttca gtgacaggtc acttcaataa acgaaagcaa
     3301 ctagtcattg aatcaatgca aattagaaac cctgatcatt ttacgcgatc gatgagcata
     3361 taaaagaaga gctgcagtcc taaatgagga ttgtagctct ttttgtttta catagaaaca
     3421 gtaaaaatga aaaggcctgt ttaggaaggc acaagcgcct tctcaattga aagacctttc
     3481 tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgctac gcgttcacta
     3541 agataaaggg tcttgcatag caagacaaaa tagaaaaaat ttcctctcac caaaaggttc
     3601 gctcctgttc gttatctctt tattgatttg tcagaccggc aacaagttgc cttttatcac
     3661 caaaaagcga caaggacaag ggtaaatggc acgctgcgcg cccttgtctt gtcgcttttc
     3721 gttgattcat tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct
     3781 ttcctaagag gaggcagaat caatcatgcg aaagaaattt gattattggg gtgttccctt
     3841 cagcgaatta ttcccaaact atcatgcacc acatactgtc gaatgcgatt gtggagaaag
     3901 agcgaaatgt atcaaaagct attgtttgta tcaatgtcca acatgcggga aaaaatatac
     3961 cttgtcttat ggcgattata ttttaattga tgagaaagga aagaaacgct gatgaaaaca
     4021 caacacaaat tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt
     4081 tctgaaaaag tagcagaagt tttacttatt ccgcggtact ggacgataga aaccccttta
     4141 gtagaggatg caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg
     4201 cattatcaag aaaacttgga aacttatgaa aacgaatatt ggttaaccaa attagagaac
     4261 taaaaaggag aaaaaaatat ggctcaaaat tactatgacg aatttgtcaa attacctttg
     4321 gataaaatgg cacaaaaaat ggaggatatg acgttcctat acaacgaaac acgtgttcca
     4381 aaaaaacatt ataaagaaaa attgtcggta gctgtggaag aaatgattga ataaggtgtc
     4441 gaaatgaatc ttattgcgac gtactatcga acactggaag aactgaaaaa gcaaaatgca
     4501 aaatggtttt tccaagcctt actatgcttg gaagtaggtg tcaaaccaag cactattaaa
     4561 ccttctgaat accaagcttt agaactaacg tatgcgaaat ttatcgaaac gaaaaaagcc
     4621 aagactgttt caagtgaatg gttagattat tttgaaaata tcaataagta tggcgcatat
     4681 tacaccatga aaaaggaaga caacgaaaat gaataaggtg atttatcaac taacaacgga
     4741 ttccatatca acgatttgaa agaaaaaaag gtcaataatc atttataccg aaaagtgaga
     4801 atgtgtgaaa caaggacaaa aaaagcctag taactaagga aatggttctt atttcgcacc
     4861 aatcgctcca accgcccccg ccgcttaggt ggtgcgaaat aaacgggcaa agcccgataa
     4921 cttaagagaa agaaggtgat ccatttgaac aagattattc ccttattgtt agtactgttt
     4981 gttggcgaaa tacttatgcg agatagtgca gctgtacagt taaacctcgc tgtatttttg
     5041 gtaatactgg tgttttgttt gatcttttgg cggtttgtat tagtcctcgt gagtctacta
     5101 ttcaagatga ttgcagccat atttttcata gggttatttc gatgaaagga gtgagtacca
     5161 atgccaattg caataggaaa taaacggtta cccgtgacat tagatgaaaa gagacaaaaa
     5221 gaattgcagc aactaaagca gaagtatggc aaaagtgaat ctaagattat gtgtattgcg
     5281 ttagatctat tgatcgcgca agaaaaagca ggatttgatg taccagcgct aaaaaagtga
     5341 cgtcactttt taatttgatc tatataagtg atatcacttt taccaaggag ggaattaaat
     5401 gttaaaaact aaaaagaatt cgtacaaatt aaagcttagt atactatcta ttatttgtct
     5461 tttaggcatg ataattagtg gtcatttgta tagagtggca tatacgcagt ttattgagta
     5521 caaggttcac tctttaggat tagctgggga tcgatacgtt gaatatgtcc attattcatt
     5581 aaaatgttct tcatatattc ttctttggct tattttttca ttgtttgaat tgctaaattt
     5641 aatagcaata tcttcatatc acttagataa gctaaatacc aaatctagcc gttataacga
     5701 agtgtttaag gaaaatgtga agttagaaag tgacttaaac aagctaaaaa aataaaaaga
     5761 agaaaacata agttaactaa aattatttta ttaagaatga tggaatataa ggataaggtt
     5821 accaacataa cgatcattac ttctctgata tgctatggac ttctatcaat cagtattttg
     5881 tctatagatt atggagtaac tatactgcaa gccttagttt tggctatgcc tatgtatcta
     5941 tctttgttta tcatgcatga aatatataag ctatttgttt tatcaaaaaa tgtagaaata
     6001 cgggtaaata ttttggtgaa tagttatcaa aaagaactca aagaaagaag acggtggcgg
     6061 tatttattga aaggatgaaa aagtaggatt tgacatactg gtattcagaa agtgccgtca
     6121 cttttaaaac aatggaaaag gtggagataa gatgaagaaa aaacaaacta aactatcatt
     6181 agcagatatt taacacagtt aaccgcaact gaagatggtg ttgttgaatt tgaacaatct
     6241 gaaattgcgg tagtagatga tcgttacctt aaaatgcctt acttctttga ccaagcaaag
     6301 gtcatttgcc tttgtcacta cgatggggta cgtgatttta ggcgttaaaa agtagtgatt
     6361 atatcaactt tacgagtaaa tataaattta gacatgaaac tgaattatcc aatggaaaat
     6421 catggtttat aatctagaaa aattatagta atgattggat tatatatatt ctttcgtatt
     6481 ttcgtacaac tgtagcttgt caaaattgag aaatttaacc gattaggtac tttacatcgt
     6541 tatttgggta atttctaaaa catgaagctt accaacgaat aaactgagac tatcttatta
     6601 attgatcaat ataagaaaat atctttgagg agatgatgta ctatgttttc gtctatccac
     6661 gattggttag gtggtctttt cggcggtgga ggtaaaggtt tttaata
//
LOCUS       DAYYNG010000148         6569 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000148 DAYYNG010000000
VERSION     DAYYNG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.150"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..950
                     /gene="sufC"
                     /locus_tag="ACHBFA_002440"
     CDS             177..950
                     /gene="sufC"
                     /locus_tag="ACHBFA_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9395864.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            963..2249
                     /gene="sufD"
                     /locus_tag="ACHBFA_002441"
     CDS             963..2249
                     /gene="sufD"
                     /locus_tag="ACHBFA_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9395865.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2246..3481
                     /locus_tag="ACHBFA_002442"
     CDS             2246..3481
                     /locus_tag="ACHBFA_002442"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9395866.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3468..3938
                     /gene="sufU"
                     /locus_tag="ACHBFA_002443"
     CDS             3468..3938
                     /gene="sufU"
                     /locus_tag="ACHBFA_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9395867.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3943..5334
                     /gene="sufB"
                     /locus_tag="ACHBFA_002444"
     CDS             3943..5334
                     /gene="sufB"
                     /locus_tag="ACHBFA_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9395868.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5432..>6569)
                     /locus_tag="ACHBFA_002445"
     CDS             complement(5432..>6569)
                     /locus_tag="ACHBFA_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9395869.1"
                     /translation="TFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAEAQ
                     TALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAF
                     GELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLP
                     RKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDID
                     YFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILY
                     GYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFES
                     GASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         2099 a         1266 c         1402 g         1802 t
ORIGIN      
        1 agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta atttagaaat
       61 caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt cattgcgctt
      121 cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta taatgcatgt
      181 ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc cttaaaggcg
      241 taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac ggcacaggaa
      301 aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca gaaggcgaaa
      361 ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg cgacttggct
      421 tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg gaattcatgc
      481 gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg gcatttttga
      541 aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct gaacgttatc
      601 tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa ttgttgatgt
      661 tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc gatgctttga
      721 aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca ttgatcatca
      781 cccactatca acgattattg aactatatca cacctgatgt cgttcacatc atgatggaag
      841 gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca gaaggctacg
      901 ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa gggggagcaa
      961 gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt tctttaagta
     1021 aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa gcagatgaac
     1081 tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt gatgtgaaag
     1141 aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg aaagacaatc
     1201 ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg gaattagcag
     1261 aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca gaactagtcc
     1321 aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact gcactccatg
     1381 tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg atcgatgaac
     1441 cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc aagcatgtgt
     1501 tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag acaaccaaag
     1561 aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa gctggttcaa
     1621 aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat atgaatcgcc
     1681 gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg aatgacggtc
     1741 atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca gaagtcaaaa
     1801 tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta accaataaag
     1861 cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa ggaacattga
     1921 cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc caacaagaaa
     1981 gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt cttttgatcg
     2041 atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat ccagaagaaa
     2101 tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta gtgattcgag
     2161 gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa gaattagtcg
     2221 aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca atgattttcc
     2281 tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg cagcgacgac
     2341 ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg ataacgccaa
     2401 tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg aacaagcaag
     2461 agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt ttactcgtgg
     2521 tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata tcaaagcagg
     2581 agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt ggcagcagtt
     2641 ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag gcttcctgga
     2701 tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca ttgctcatgt
     2761 atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg tccatgaaaa
     2821 cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca ttgatgtcca
     2881 agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac caacaggtat
     2941 tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg aatttggtgg
     3001 cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac cttggaaatt
     3061 cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa tcgattattt
     3121 gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg cttatgtgtt
     3181 gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc cgaattttcg
     3241 gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg cgacagcttt
     3301 agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc tattgaagta
     3361 ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa cgcaagacgc
     3421 cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg ggactatcta
     3481 aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg catcatcgtg
     3541 gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt ggagatgtga
     3601 tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt gacggcagtg
     3661 gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc ggaaaatcaa
     3721 tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga aaagaagtca
     3781 aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa tttcccgctc
     3841 ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa aacgatggta
     3901 ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt accagaatta
     3961 gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac aggagaaggc
     4021 ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga atggatgctt
     4081 gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac atggggcccc
     4141 gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag tgacaaaccc
     4201 gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa gattggtata
     4261 ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc agaagtcgtg
     4321 taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga cactgactct
     4381 gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt accgccaacc
     4441 gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt tatttacgtg
     4501 ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa tgcggaaaac
     4561 acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat ccactatgtc
     4621 gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat cgtagaaatc
     4681 tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc agacaatgtg
     4741 tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga atggatcgac
     4801 ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga tggaaaaggt
     4861 gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca agatacgggt
     4921 gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa atcaatttca
     4981 aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa cagtgcgggt
     5041 tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa atccgatacg
     5101 atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc caaagtctca
     5161 aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga atctgaagca
     5221 actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc aatggaatat
     5281 gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg ttaataacta
     5341 tttcattgct cgaaacgttg atattaaagc gtttcgagcg tttttttatt tgtattttaa
     5401 tggttggcta cgtttttggc tacgtttgga attattcgcc cataaatttt gcaaatcggt
     5461 caccagtttt cttaacagat tgtggtgtga catgagcata gatattcatg gtagttttaa
     5521 tatctttatg acctaatcgt tcctgtactt cttttatcga cgcaccactt tcaaacaata
     5581 agctacagtg ggtatgccta aatccatgtg gagtaatttg aggctccatc ggtgtctttt
     5641 tgtaaatcca gtctaaccaa tcgtttacta cttgggggta gtacaacttg tttgtgtttg
     5701 tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc atataagatc attattttca
     5761 tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa atcatcaagc tgaatcgttc
     5821 ttgatgaatt tttagtttta ggtacttgta gaactacttg attaaactcg tcttgagcga
     5881 tagttttacc aattgttatt gttttattga aatagtctat atcttcccat tgaagcgcca
     5941 atgcttcgct tttacgcatt cctgtataag ctaataaacg gaaaaaagtg tacagtttaa
     6001 ctgaattatt ttctttagta ttctgtaaaa acagttgtaa ttgatctttc gtgtaaaact
     6061 tcaagggttg ttcttcttca atttttcttg gaagcaatgt tttagacata ggattatcct
     6121 tacagacttc cattgctact ccaaatttaa atatttgagc agttgttttt ctcaagaaac
     6181 catattgttt taatgttttg tgccattcat tcacctgttt ttggcaatag ctaacactta
     6241 ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg gtttttgcaa aaacgttgag
     6301 caacggcaac agaagacggt tttactcttg ttctgtagtt atcaatccat aattcataca
     6361 actcttgaaa agttgtaaca ttgattttaa actcttgttt ttctttaaag tctacaagta
     6421 gtctattaag tgctgtttgc gcctcagctt ttgatttgaa tcttctacgc tttgtacgta
     6481 tatcttttcc agtcttggga tctttcccta aataagtttc aaatccccaa tatttttcgc
     6541 cattttgtaa tttataacta aaaaatgtt
//
LOCUS       DAYYNG010000149         6547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000149 DAYYNG010000000
VERSION     DAYYNG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.151"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHBFA_002446"
     CDS             complement(1273..1938)
                     /locus_tag="ACHBFA_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395870.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACHBFA_002447"
     CDS             2064..4100
                     /locus_tag="ACHBFA_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM9395871.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACHBFA_002448"
     CDS             4104..4742
                     /locus_tag="ACHBFA_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9395872.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHBFA_002449"
     CDS             4770..5198
                     /locus_tag="ACHBFA_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395873.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..>6547
                     /locus_tag="ACHBFA_002450"
     CDS             5655..>6547
                     /locus_tag="ACHBFA_002450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9395874.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDS"
BASE COUNT         2242 a          995 c         1212 g         2098 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctag
//
LOCUS       DAYYNG010000150         6527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000150 DAYYNG010000000
VERSION     DAYYNG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.152"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..674)
                     /locus_tag="ACHBFA_002451"
     CDS             complement(<1..674)
                     /locus_tag="ACHBFA_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9395875.1"
                     /translation="MQAPIKKICVHFETKQWRNKMVATVTLYLTFTLILDLGIYLFFS
                     KQLLVKNSFGCIALMVVTLLLPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFF
                     AITGLSFFLVFDLMDYLGKRHLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPT
                     EHYFGGICILLSWGVIYLVLGSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKE
                     RQHQESL"
     gene            675..1415
                     /locus_tag="ACHBFA_002452"
     CDS             675..1415
                     /locus_tag="ACHBFA_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9395876.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1501..2259)
                     /locus_tag="ACHBFA_002453"
     CDS             complement(1501..2259)
                     /locus_tag="ACHBFA_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9395877.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2256..2720)
                     /locus_tag="ACHBFA_002454"
                     /pseudo
     CDS             complement(2256..2720)
                     /locus_tag="ACHBFA_002454"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(2739..3236)
                     /locus_tag="ACHBFA_002455"
     CDS             complement(2739..3236)
                     /locus_tag="ACHBFA_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM9395878.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(3304..6471)
                     /locus_tag="ACHBFA_002456"
     CDS             complement(3304..6471)
                     /locus_tag="ACHBFA_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM9395879.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
BASE COUNT         1835 a         1390 c         1156 g         2146 t
ORIGIN      
        1 agcgattcct gatgttgtct ttcttttact tggaaactga tcaacataat ggaaaaaaac
       61 aaagaaaaaa aactgatgac tatcgacaaa agagatagaa gcagataatt agatgagcgg
      121 aagtgaacac ttcccaatac caagtaaatc acgccccagc ttaataaaat acaaatacct
      181 ccaaaatagt gttcagttgg ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct
      241 atacttttta ctaatagata gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt
      301 ccaagatgtc tctttcctaa ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca
      361 gtgatagcaa aaaaaagatt actgatcaaa cagctgataa acaggttttt cgtagtaaaa
      421 taaaaaagaa acatcaataa taagccaact aggtaaggaa agctcacttt agggatagga
      481 agtaaaagtg tcaccaccat caaagctata caaccaaagc tgttttttac tagcagttgt
      541 ttcgaaaaga ataaatagat acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc
      601 actgttgcga ccatcttatt cctccattgt tttgtttcaa aatgtacaca tattttttta
      661 ataggagcct gcatatgaaa aagtatttta tcatagaaga taaccagttt cacagcgaat
      721 ggatcatcag agaattggtc aatcatcata tcgagaatat cgtgcagctc acttctatct
      781 ctcaaatcaa tcagttgatc cagttgggca ctattgataa tcacgatgtt ttctttatcg
      841 atatcgactt gaagcaatca ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa
      901 atgcacatag taatattgtt tttttcaccg cttatacgaa ttttgcagta gagatcatca
      961 atcggaaaat ccttccgatt ggatatatca gaaaagacag gaatttatct gaacaaattc
     1021 gtcaagtgct agaacagatc aattggaaag aacagcagat cgtaaatgtc gataaaatct
     1081 ttattgatca aaaaggtgga agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa
     1141 gtatcaaagg tttgaaaaac caagttatgg tcaaaacaac tacagaagat aaattagtga
     1201 atacttcttt aaaacaattg aagacagagt tagaaaagta tgcttacttt ttacttttaa
     1261 aatcttactg tatcaactta tcgcaattgc agaaaattga ttctgctcat tatgtgttag
     1321 aatttttcaa cggtgactcc ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac
     1381 gtttccatga cttccaaaaa actgcttcga gttgatgaat aagagctgag acgtaagcat
     1441 agcagatttc agggagtggg ccttgaatcg gctccactcc ctgaaatcgt tgatatgatt
     1501 ttacgcttcc tcttctgtag gagcatctgt taacgaccat gtgaatgttg aaacgtagtt
     1561 gtctacagca taattggata caggaacaaa taattccact gattgtctcc cttgttccac
     1621 attctctcct aatgaataaa tccatgtgcc aatcccttca tttgctcctg cagttactag
     1681 atctactgcc tgatctgctg ggatttcgat tccttcagcg ccagttccag aaacggtaga
     1741 tggcattcca ctgctattag ttgcgctatt tcgaatcacg gcgtttttga atttcacctt
     1801 tgaaccagtt agtttattcg tatcagatgt tgtaccaata aattctgttc gtttgactga
     1861 taatttccaa ccggcataat ttccgctttt atccgttact tgtacgaaat tctgtacttg
     1921 tgtttgtgct ccagtactgt ccgttccgct ttgtaatgca gcatatactg tttttgctgt
     1981 acctaacttg attgttccaa aatcaagatc tggagcttgg tcaatcgtca gtaatccgga
     2041 tgttccctga tttggttcgt caggtgtaaa aggagtatca ggatcatttg gatcaacagg
     2101 aggaactgga acaacgtctt caggatttgc agcatctggt ttggtcacag aaatcgttcc
     2161 ttctgtagtg gctgagctct tattctcagc aaaaacactt tgtgtacata ataatagact
     2221 tccagctaga acggtactta atgcaattgt ttttttcatt tctttttcct tctttctttt
     2281 aaaataaata atccagcaaa taagccaata agaatgactc caacaagtat cagccatcgc
     2341 cagtcaaatg tttctggctg attttttaaa tcaatatcct tttcattcaa ttggtccgct
     2401 gcatcatcag ttatttcaaa atcggtatgg agattccagc gtttttcttt ccaagcaccc
     2461 ttcacaaccg cttgataatg tcccggagct aatttttttc cttctaatgg gacaagtaaa
     2521 tcaaaatttg tattgggtgc tacatctatt ttttcttttt ttattgaaag gtaaggctct
     2581 tttgtttctt ttttgtagat ttcaaccgat gtagtcaagc cgaacatata agaagcattt
     2641 ggattttgaa gattcgcctt aatcacattt cttccatcgc tttgatccgc ataagctttt
     2701 tttaatttca aatctgggga agactctttt tcgctatttc aaatcaagat tgctttcact
     2761 tgagaaaact gattgacgac accaactgta cttttgttag aaacgttctc ttttttttct
     2821 tgaaacgtga tccctcctgc gaccacacca gccagctctt tttctggcgc tttcagtgaa
     2881 agtgtctcag tgaccgcact ttgaggagga acagttattt ctcccactgt ttttgctaag
     2941 tttttcatat cataacttaa tgtttcatca aatttacttt ttgattctcc atattcgatc
     3001 acaccgtttg aattcgtcgt agcggagtgg atcaatgtct caactgttac tgatttatct
     3061 gttcggttaa agaccgtgta ggggatttcc tgtgttttcc caggctccat ccttacattt
     3121 atatatgttt tgttcttatc aatctgatta gccggtaatt caaaatctac cgcaaaattg
     3181 aattcggagg catggacagg tgtctgccaa aggatgaatc caaggagaag gatcataagt
     3241 aaagagcagt tgtttcgatg acttttattc catattcgat tcatcaatca tcctccttga
     3301 tctttattct gtcggcgcat ctgctaatgt ccatgttatc tctgctgagt aattaccagc
     3361 tatctgattt tctttggtta agacaagttt aaacgcccga ccgttattta aatattctga
     3421 ataatctgct tgcacattcg ttgaattttg tcctctgaca atttccagtt cttcttcatt
     3481 cactaattga tactcctctc ctttcttgaa atataggaca tcctgcatag aaaatcctgc
     3541 atcatttgtg aaaggctgag ataattttat cgttaagatc cacggatttt tcgtttctcg
     3601 ctgatcataa atccataatt tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa
     3661 accagttgct ttgatctgac caaaatcgag tttggaaggg gcaagaaatg agagaccacc
     3721 ttctactacg atcttcgtag caggtgcaaa aggcggggca tcatgataaa caactggcgt
     3781 atcacttgga ttgtgattcc caatttcatc gttggtactt ggcttgttct gcacaccctt
     3841 agcagtagct tctcctgctt ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag
     3901 ttgattttct gggatagaaa gaacaaattc ccctgtatca tcaatagttg tttctgacaa
     3961 aacagcttga ttttttttca atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt
     4021 gcgagaagct gcattgatca atgtggtact taaattagca ggttcaggag gaatcacatc
     4081 gactgtcgtt ttggcagagt ccagttctgt cacttcttgg ccacctctta cttttttagc
     4141 agcaacaact tgcacttgat cctccgtttt taatctctca tcattgggta cttgaacttc
     4201 gaacaagccg ccttgttcgc cttctcccca tgggttatat cctgcccctt cattttcatc
     4261 tgtttgtcct tgcgtgagag cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt
     4321 aatacgtaaa gtcacttcta cttcgcctgt ccaagcatct cttggctttg cctcgacacc
     4381 ttcgggaaca acgacatgcc catagatttt tttatctgca ttcgttggca ctcttaagga
     4441 ttcaatcatc gggagctgat tattggcact gattcgatta taatttttca ttccggcacc
     4501 tgtctgagca aattcatttg ttaaacctgc actagggctt gatacgaaat tgcctaagtc
     4561 ggtcccattg atcgataagt ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt
     4621 atcgaatgtt gcgcctgttt ttcgccataa agcgatttct gaatgggcga tctttaaaga
     4681 agaatctcca tttccttgga atacccagcc gcctatgcca ttggtctcgc ttccaatccg
     4741 tttgttttgg aaatcaaaat aggtaggaga ttctaagtcc acattcaaca catccccact
     4801 gatgatgccg gtcttatctt cccctgtttt cccgaccatg actaaataag ttttttcacc
     4861 tgccgtaaaa tttaaatcaa tatctccagt tgtatctaca gttgctccgg aatctgcgcg
     4921 tatatcgata tttgattttt ctccagtgac tgtaaaatca gctgaacctg gactttctag
     4981 aaaaccagaa gcgtcataaa attggatcgc ttgatttcct tgattgagtc tgttcacgta
     5041 agatttgcca tcacctgtat ttcttaccct caatgagcct ccattggtta ctgtcagctt
     5101 ttgcgtacca ttctcaaaac gaactgcgct ggaacgccca ctctgcttat cgatgtcgat
     5161 aactgatccg gctccttcta cgatcaaaga aaggcctttt cctacaaaac gcatcccatt
     5221 gttcccgggt tgatcgtcat ctccagccat tttgaccgag atttgtccac cacttgatac
     5281 atggatttga ctagaatctc cttgcaagcg aaatccgccc gtagttgctg tattgatggt
     5341 gattttccca ccattcgata cagtgacttt cgatcctgaa cctctcattt ggatgattcc
     5401 gcgattgttg tcattggtaa tattgatctt tgtatttacc gaaaattcgc ttcccgaacc
     5461 actgacttca aacatgacat taccaggtag cgtatcgact gcctctcctt catcgctaac
     5521 gccatctgcc cgatcataat caagagagac ctttgaaccg cttaagatat ggattcctgg
     5581 cgctgttttt ccactaccag tctcatctcc tgaatctaga aaaccgtaga atttccctag
     5641 atacggtcca taaagttgac taccattgat cacattcaat tgatagcttc tacaaaaata
     5701 ggctttaccc ggagcggcat tcagtccttc cttcactggt tggacatcca acacaccgga
     5761 aacaccatct aatgtgacag ttgcacgagg agcgtaaatc agtcctaatt tggacccatc
     5821 atacaaatca agtgtcccgg taaattttat ttctcccgta aaatgatatc tgcttcccca
     5881 aaaatcggtc atataagcaa tgataatggc atttccatcg atatttggtg tcaatccacc
     5941 tggatgtgca gctgttcctt taaaattctg gaactcgatc gactgaggta tctcattgat
     6001 ataaatatgg tgtgtgccga aattgtgagc gaatccacca aaatcaataa cgatatctcg
     6061 tttgatctct aattccctgt ctaaagtcaa aggctgaatc agtcggatct tagttactga
     6121 ttcgtctgcg agaagctgcg ccagttcttc tgttgtggca gcttcgctgt acccatccgc
     6181 tgaaacagct cttgccagtg ccgcttcact gctatcagac gtttgttcag ctacttctga
     6241 ggtctcactt gtcttatttt cttcttgcct tttattagta gaagcctcac tttgagattc
     6301 tgtagtttca gaagtaatcg attcttctga aaaagtagtc ttgtcaaaac tttgattctc
     6361 tgattgcgtt gattcgtttg ttttctctac atcggcaaaa acggatgtca ccatcaagag
     6421 agggaagaca aacaaaatcg acgcaatcag cttttttaca cattgattca ttctccacca
     6481 tcctctctga cgttttgaca attttatttt atcgttttaa tctattt
//
LOCUS       DAYYNG010000151         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000151 DAYYNG010000000
VERSION     DAYYNG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.153"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHBFA_002457"
     CDS             334..714
                     /locus_tag="ACHBFA_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9395880.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHBFA_002458"
     CDS             696..1610
                     /locus_tag="ACHBFA_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9395881.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACHBFA_002459"
     CDS             1855..2544
                     /locus_tag="ACHBFA_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395882.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHBFA_002460"
     CDS             complement(2665..2751)
                     /locus_tag="ACHBFA_002460"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9395883.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHBFA_002461"
     CDS             3427..4377
                     /locus_tag="ACHBFA_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9395884.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHBFA_002462"
     CDS             4398..4931
                     /locus_tag="ACHBFA_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9395885.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHBFA_002463"
     CDS             4947..5117
                     /locus_tag="ACHBFA_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395886.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHBFA_002464"
     CDS             5567..6100
                     /locus_tag="ACHBFA_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395887.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHBFA_002465"
     CDS             6112..>6303
                     /locus_tag="ACHBFA_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395888.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAYYNG010000152         6263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000152 DAYYNG010000000
VERSION     DAYYNG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.154"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHBFA_002466"
     CDS             complement(99..1229)
                     /locus_tag="ACHBFA_002466"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM9395889.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHBFA_002467"
     CDS             complement(1878..3080)
                     /locus_tag="ACHBFA_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9395890.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHBFA_002468"
     CDS             complement(3140..3343)
                     /locus_tag="ACHBFA_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9395891.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHBFA_002469"
     CDS             complement(3485..4552)
                     /locus_tag="ACHBFA_002469"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9395892.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHBFA_002470"
     CDS             complement(4610..4717)
                     /locus_tag="ACHBFA_002470"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395893.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHBFA_002471"
     CDS             4880..5692
                     /locus_tag="ACHBFA_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9395894.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHBFA_002472"
     CDS             complement(6066..6257)
                     /locus_tag="ACHBFA_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395895.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1857 a         1285 c          996 g         2125 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tat
//
LOCUS       DAYYNG010000153         6256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000153 DAYYNG010000000
VERSION     DAYYNG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.155"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..228
                     /locus_tag="ACHBFA_002473"
     CDS             <1..228
                     /locus_tag="ACHBFA_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9395896.1"
                     /translation="AIEVVGTFIWLTGNTKPYKEDIKALEFRYSPKKYAWYKAPKDYK
                     KRSRLRSICEKRLSLRQLHHILVVNMYLKYT"
     gene            complement(313..909)
                     /locus_tag="ACHBFA_002474"
     CDS             complement(313..909)
                     /locus_tag="ACHBFA_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9395897.1"
                     /translation="MDGFERRTEEKKKKVLRAAFDLMNTDTGIENLTIDAVAKKANVG
                     KTSIFKYFGSKEKLIHEVFIRSMDEMGEKAKKIMEKNASFEETLIAMSQNKIDYLEKI
                     NKQFYLDLMSYYTKKEDSGFTRMMEAYTKQSFGIMLDLFHRGRKEGKVDLKYSDEFLM
                     LYFQAMIEGISNPQIYEKILPYTAEWTELLVKGIAPRQ"
     gene            1220..2101
                     /gene="narA"
                     /locus_tag="ACHBFA_002475"
     CDS             1220..2101
                     /gene="narA"
                     /locus_tag="ACHBFA_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionophore ABC transporter ATP-binding subunit
                     NarA"
                     /protein_id="HFM9395898.1"
                     /translation="MTEIVKVQGLQKKFGKFQALKDVSFTVNAGEVVGFIGPNGAGKS
                     TTIRTLLGIINRDEGDVQIFGKDVWKDSLEIHKRISYVPGDVALWGSLTGGEIIDLFI
                     KLHGGGSKAKRDYLIKRFELDPKKKAKGYSKGNRQKVGLIAALSVESDLYILDEPTSG
                     LDPLMEAVFQEEVEKIKNDGKAILLSSHILSEVERLADKVAIIRRGEVVETGTLDELR
                     HLTRSTVTLVTEGDIEKLATLSGVHDFVQKDGKATFSADNEAMNTILTEATKLGVTKI
                     ESVPPTLEDLFMRHYEG"
     gene            2125..3738
                     /gene="narB"
                     /locus_tag="ACHBFA_002476"
     CDS             2125..3738
                     /gene="narB"
                     /locus_tag="ACHBFA_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionophore ABC transporter permease subunit NarB"
                     /protein_id="HFM9395899.1"
                     /translation="MNEKFARWNVLFIQYVKRDWKKIIVWVLGLGLFSGAFVPAFEEI
                     AKGQGLLGMFETMQNPAMISMVGPTPIKIGTDYTLGAMYAQEMLLFCGLFAMIISALH
                     VVSHTRKEEELGLTELVRSFRVGRQANSLAVISEMLLINLLLGLLIGGLMMSFGVKTI
                     DAEGAFLFGGSIALAGIIGGVLALVMSQIMATSTGATGSTLSLIGLLYIVRAGTDVSN
                     LDLSMFNPMGWIYLTYPFTENNWLPLLFALIFSLVFTVLAFVLEEHRDMGAGYLPERE
                     GRATAKKSLLSVPGLFFKINKGVMIGWLIAFVVMGAAYGSIYGDMQVFLGGNELMKQM
                     FTQSGVSIEESFTATIMMVMIGLVTILPIAVVNKLFAEETRLHLSQLYVTKITRGQLY
                     WTTIFLAIFAGVVGIGLASAGLGGTAISAMKNESTMDLTDFLAAGYNFLPSILFYIGL
                     AALALGWLPKFGKVIYAYLGYSFALNYFGGILDLPDWFSKTAIQSWIPRLPMEKFDGT
                     IFAVITVISIVFLFVGYLGYKRRDMVEGA"
     gene            complement(3810..4400)
                     /locus_tag="ACHBFA_002477"
     CDS             complement(3810..4400)
                     /locus_tag="ACHBFA_002477"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9395900.1"
                     /translation="MVGKFLTTISCACFGGALAVIGSSAYNKIHERPSAANERLEIGN
                     WEADTVAGKTGRARIVTLVDRRSRFSLLGKVEKKISKNVVDCMINLLRNVGEDKCKTV
                     TPDRGKEFSQHSRITLELNGTKVYFPDPHAPWQRGTNENTNGLLREYSPKGEDISGIF
                     DSQIQEWANKLNTRPRKCLNWKTPYEVFFNESLHLI"
     gene            4321..4701
                     /locus_tag="ACHBFA_002478"
                     /pseudo
     CDS             4321..4701
                     /locus_tag="ACHBFA_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
     gene            complement(4964..5704)
                     /gene="erm(B)"
                     /locus_tag="ACHBFA_002479"
                     /pseudo
     CDS             complement(4964..5704)
                     /gene="erm(B)"
                     /locus_tag="ACHBFA_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
     gene            complement(5826..5909)
                     /locus_tag="ACHBFA_002480"
     CDS             complement(5826..5909)
                     /locus_tag="ACHBFA_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM9395901.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            complement(5958..6037)
                     /locus_tag="ACHBFA_002481"
                     /pseudo
     CDS             complement(5958..6037)
                     /locus_tag="ACHBFA_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
BASE COUNT         1953 a         1074 c         1186 g         2043 t
ORIGIN      
        1 gcaatcgaag tagtaggaac atttatatgg ctaacaggta atacaaaacc atataaagaa
       61 gatataaaag ccctagaatt ccgatattca cctaaaaaat atgcttggta taaagcccct
      121 aaagattaca aaaaaagaag ccgactacga tcaatctgtg agaagcggct gtctctgaga
      181 caattgcatc acattttagt tgtgaatatg tatctcaaat acacctagca gatgaatcga
      241 ttctgatttg tatgcataca ttttattttt ttgtggattc ggcgagtatt tcttcttttg
      301 ttgttcgtaa aactattgtc tcggagcgat tccttttacc aacagctcag tccattccgc
      361 tgtataaggc aggatttttt cataaatctg cggattagag atgccttcga tcatggcttg
      421 aaaatacaac atcaaaaatt catctgaata ttttaaatcg acttttcctt ctttacgccc
      481 gcgatggaat aaatccaaca taatgccaaa actttgcttt gtataagctt ccatcattcg
      541 ggtaaagccg ctgtcttctt ttttagtgta atagctcatt aaatctaagt aaaattgttt
      601 attgattttt tcgaggtaat cgattttatt ttgactcatg gcgatcaaag tttcttcaaa
      661 tgatgcattt ttctccatga tttttttagc tttttccccc atttcatcca ttgaccggat
      721 aaagacttca tgaatgagct tttctttgct gccaaaatat ttaaaaatag aagtttttcc
      781 tacattggcc tttttggcta cggcatcgat cgttaaattc tcgattcccg tatctgtatt
      841 catcagatca aaagcagctc gtaaaacttt tttctttttt tcttctgttc ttcgttcaaa
      901 accatccatt aaaaatacct actttctata aaaaagaact ttttgtttat catagttcaa
      961 aactagcttc gtatatatta tataggaaag aatacgaact aacaatggaa tcataggttt
     1021 aaatttaaaa ttatttttat tgactgttaa aataaaaaag aatattattg gagctgtaaa
     1081 agaactttgg tttgaaacaa agtataaagg ggtattttat gaacgataca tattgtatta
     1141 gttcataaaa aaagtgcgtt ttgttcagac tggaaagctc gttataaaat catgtcaaaa
     1201 gaaaagggag ttatgtacca tgacagaaat tgtaaaagta caaggcttgc aaaaaaaatt
     1261 tggtaaattc caggcgttga aagatgtctc attcacagta aacgccggtg aagttgttgg
     1321 ttttatcgga ccaaatggag caggaaagtc aacgacgatc cgtacactgc taggaatcat
     1381 caacagagac gaaggagatg tccaaatatt cggaaaagat gtttggaaag atagtctaga
     1441 aatccataaa cgaatttcgt atgttcctgg agatgttgct ctttggggca gcctgactgg
     1501 tggagagatc attgatctat ttatcaaact tcatggcggc gggagcaaag caaagcgcga
     1561 ttatttaatc aaacgatttg aacttgatcc aaagaaaaaa gccaaaggtt actctaaagg
     1621 aaatcgtcaa aaagtcggtt tgattgctgc actttcagtt gaatctgatc tgtatatttt
     1681 agatgaaccg acttcaggac tagatccatt gatggaagca gtattccaag aagaagtaga
     1741 aaaaatcaaa aatgatggca aagcgattct attatcttca catattttaa gtgaagttga
     1801 acgattagca gataaagtag caatcattcg acgtggagaa gtagttgaaa caggtacatt
     1861 agatgaattg cgtcatttga ctcgctcaac agttacattg gtgacagaag gcgatattga
     1921 gaaacttgcg acgctctctg gcgtgcatga ttttgttcaa aaagacggca aagcaacttt
     1981 ttctgctgac aatgaagcga tgaatacgat tctgaccgag gcaaccaaat taggtgtgac
     2041 aaaaatcgaa tctgtaccgc caacgcttga agatctattc atgcgtcatt acgaaggctg
     2101 attgtcggaa cggaggaaaa gaaaatgaat gaaaaatttg cccgttggaa cgtattgttc
     2161 attcaatacg tgaaacgcga ttggaaaaaa ataattgttt gggttttagg tttgggtttg
     2221 ttctcaggag catttgtacc agcatttgaa gagattgcta aaggacaagg tcttttaggg
     2281 atgtttgaaa cgatgcaaaa tccagcgatg atctcgatgg ttggacctac accaatcaaa
     2341 ataggtacgg attatacttt aggagcgatg tatgctcaag agatgttgct gttttgcgga
     2401 ttgttcgcaa tgattatctc agcacttcat gtggtgagcc acacgcgaaa agaagaagaa
     2461 ttaggtttga ctgaattggt tcgctcattt cgagtaggac gacaagccaa ttcattagct
     2521 gttatcagtg agatgctgtt gatcaatctt ttattaggtc ttttaatcgg cggactcatg
     2581 atgagttttg gtgtaaaaac gattgatgcc gaaggagctt tcttgttcgg aggatcaatt
     2641 gcattggcgg gaattatcgg tggtgtattg gcacttgtga tgtcgcagat tatggcgact
     2701 tctactggag caaccggctc gacattaagt cttataggac ttttgtatat cgtgcgcgct
     2761 ggaacagatg tgtctaatct tgatttatca atgttcaatc caatgggatg gatttacttg
     2821 acctatcctt tcacagaaaa taactggcta ccattattat ttgctttgat ttttagtctt
     2881 gtttttaccg tacttgcgtt tgtgttggaa gaacatcgcg acatgggcgc aggttatctt
     2941 cctgaacgag aaggacgtgc gacggcgaag aaatcactac tttctgtacc tggtttgttt
     3001 ttcaagatta ataaaggagt aatgattggt tggctgatcg catttgtcgt tatgggagct
     3061 gcgtatggct ccatttatgg agacatgcaa gtctttcttg gcggaaatga actgatgaaa
     3121 caaatgttca ctcaatctgg cgtttccatt gaagaatcct ttacggcaac gatcatgatg
     3181 gtaatgattg gattagtcac aatcttgcca atcgcggtgg tcaataaatt atttgcagaa
     3241 gaaacaagac tgcatctgag tcaactgtat gtaacgaaga ttacgcgagg ccaattatat
     3301 tggacaacga tatttttagc tatttttgct ggagtcgtag gtattggctt agcatcagcg
     3361 ggattaggtg gaacggcgat ttctgcgatg aaaaatgaat cgactatgga tctgaccgat
     3421 ttcttagctg ctggatacaa ttttctccct tccatcttat tttatattgg tttggctgct
     3481 ttagcgttag gctggttgcc aaaatttgga aaagtaatct atgcttatct aggctattcc
     3541 tttgctttga attatttcgg cggaatctta gatttgccgg attggttctc aaaaactgca
     3601 attcaaagtt ggattccacg cttaccgatg gaaaaatttg atggaacgat ttttgcagta
     3661 attactgtta tcagtatcgt cttcttattt gtcggctatt taggatacaa acgccgtgat
     3721 atggtagaag gcgcttaaaa attgaatttc taaaaaaccg ttcatattta cgaaagtcga
     3781 tttgagcggt ttttttatgg cttcaattgt caaattaagt gcaacgattc attaaaaaat
     3841 acttcataag gggttttcca atttagacat ttccgtggtc gcgtgtttaa tttattcgcc
     3901 cattcttgaa tttgactatc gaaaatacct gaaatatctt cacctttggg tgaatattct
     3961 cgtaataaac cattagtatt ttcgtttgta ccacgttgcc atggagcatg tggatctgga
     4021 aaatacactt tagtgccatt taattctagc gttattcgac tatgttggga gaattctttt
     4081 cctctatcgg gtgtaacggt tttacattta tcttctccta catttcttag tagattgatc
     4141 atacaatcaa ccacattttt agagattttc ttttcaacct taccaagtag ggagaaacga
     4201 gatctacgat cgactagagt tacaatacga gccctacctg ttttaccggc tacagtatca
     4261 gcttcccaat taccaatctc aaggcgctca ttggcagcag atggcctttc atggatctta
     4321 ttgtatgcag atgatccaat gacagctagg gcaccaccaa aacaagcgca tgatattgtt
     4381 gtcaaaaact taccgaccaa tttagaaacc cttcataaaa cgggcttatt tagcgatata
     4441 aggctttaca atagagaagg agtgaaactc tattcaagct tagaaacgcc ttccattagt
     4501 ccgaaagaga ccttagaaag agaattaaat cgtaaagtat cagggaaaga aattcaaccg
     4561 actttggaga gaatagagca aaaaatggtt caaaatcaac accaagaaac ccctgaattt
     4621 aaagcaattc aacaaaaatt ggaaagcttg cagccaccta caccaccaat acccaaaaca
     4681 cctaaacttc caggtattta aaccttaaaa aaggaaaaga gtagttacca aaaaacggta
     4741 actactcttt ttttataaaa acatttcctc atgttggggt cccgagcgcc tacgaggaat
     4801 ttgtatcgat aagaaataga tttaaaaatt tcgctgtttt tttctaattt cattttgagg
     4861 tgaattaatt ggtagttatt agaggtgccc tatacctaat aatttatcta cattcccttt
     4921 agtaacgtgt aactttccaa atttacaaaa gcgactcata gaattatttc ctcccgttaa
     4981 ataatagata actattaaaa atagacaata cttgctcata agtaacggta cttaaattgt
     5041 ttactttggc gtgtttcatt gcttgatgaa actgattttt agtaaacagt tgacgatatt
     5101 ctcgattgac ccattttgaa acaaagtacg tatatagctt ccaatattta tctggaacat
     5161 ctgtggtatg gcgggtaagt tttattaaga cactgtttac ttttggttta ggatgaaagc
     5221 attccgctgg cagcttaagc aattgctgaa tcgagacttg agtgtgcaag agcaacccta
     5281 gtgttcggtg aatatccaag gtacgcttgt agaatccttc ttcaacaatc agatagatgt
     5341 cagacgcatg gctttcaaaa accacttttt taataatttg tgtgcttaaa tggtaaggaa
     5401 tactcccaac aattttatac ctctgtttgt tagggaattg aaactgtaga atatcttggt
     5461 gaattaaagt gacacgagta ttcagtttta atttttctga cgataagttg aatagatgac
     5521 tgtctaattc aatagacgtt acctgtttac ttattttagc cagtttcgtc gttaaatgcc
     5581 ctttacctgt tccaatttcg taaacggtat cggtttcttt taaattcaat tgttttatta
     5641 tttggttgag tactttttca ctcgttaaaa agttttgaga atattttata tttttgttca
     5701 tataatcact cctgaagtga ttacatctat aaacaaatac agaagttaaa cgatttgttt
     5761 gtaattttag ttatctgttt aaaaagtcat aagattagtc actggtagga attaatctaa
     5821 cgtatttatt tatctgcgta atcactgttt ttagtctgtt tcaaaacagt agatgtttta
     5881 tctacataac gcatttggaa taccaacatg acgaatccct ccttcttaat tacaaatttt
     5941 tagcatctaa tttaacttca attcctatta tacaaaattt ttaatgcact atcaacacac
     6001 tcttaagttt gcttctaagt cttatttcca taactttagg gttaaccata cgcaagacca
     6061 atcactctcg gacaatactc atgattttaa tgataaaatc catgttaaac ccatagataa
     6121 gaaatacacc tgcaataacc gttacctgtt tgtgccaatt taaaaatgca ccactttttt
     6181 ataattaaaa cggttgaaaa ctgtaccact aataactcac aatagagaga tgtcaccgtc
     6241 aagttaaatg tacaaa
//
LOCUS       DAYYNG010000154         6192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000154 DAYYNG010000000
VERSION     DAYYNG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.156"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1526)
                     /locus_tag="ACHBFA_002482"
     CDS             complement(75..1526)
                     /locus_tag="ACHBFA_002482"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9395902.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADCLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1847..2611)
                     /locus_tag="ACHBFA_002483"
     CDS             complement(1847..2611)
                     /locus_tag="ACHBFA_002483"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM9395903.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2639..3337)
                     /locus_tag="ACHBFA_002484"
     CDS             complement(2639..3337)
                     /locus_tag="ACHBFA_002484"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM9395904.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3349..4128)
                     /locus_tag="ACHBFA_002485"
     CDS             complement(3349..4128)
                     /locus_tag="ACHBFA_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM9395905.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4144..5088)
                     /locus_tag="ACHBFA_002486"
     CDS             complement(4144..5088)
                     /locus_tag="ACHBFA_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9395906.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5134..5913)
                     /locus_tag="ACHBFA_002487"
     CDS             complement(5134..5913)
                     /locus_tag="ACHBFA_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395907.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1706 a         1283 c         1016 g         2187 t
ORIGIN      
        1 ttccaaatct ttatcaattt ttatccactc attctaccac tttagtaata ttttctattt
       61 ataacacttt cgtcctacat tgatcccccc ctctgaagta cagtttttac tgtcttaaat
      121 aaaattttaa aatctaaccc catactccaa ttttttatat attccgtgtc aagttttgct
      181 acttcttcaa aatcagtaat atcacttcta ccacttacct gccacattcc agtaattcct
      241 ggcttaatag caattcttgc acgatgatga taatcatatt cttcatactc tgatttaagg
      301 catggtcgaa atcctataag gctcatcttt cctgttagaa cgttgaaaaa ttgagggaat
      361 tcatctaaag acgtggatct aataaattga ccgattcctg tcttttttgt tccatctgga
      421 agtattttat ttccgataat tcttggatca aactccatct taaacataag attattgctc
      481 atcttatttt ctttcattaa ttctactttt ctttcttctg catccatata catacttcta
      541 aacttataca tcttgaacgg tttaccattc tgacctatac gttcttgtgc aaagaaaatt
      601 gggcctggtg aattaatata aataagtgga gcaataaaga taaaaataat tcccgttgca
      661 atacatccaa caatacctgc aagaatatca attgttcgtt ttataaatgc ctgttgagtt
      721 gtcataacat tgatacttgt tgtaagtact gtatattctc ctatacgacc aatagattgt
      781 tttcccaaag cactatctga tacttttgca agattcaaat gtacagtaag gcccatttca
      841 gagcatcgtt caataaattc tttcatacac gaagtacttt tattcaaaat aataaagact
      901 tcatctaccc atccctgaca taagcaatcc gctgcatttt tggaatcaga aacaactgtc
      961 actccctcaa catcttcacc aatcatgctt ttatctatga taatgacacc atttatttta
     1021 tacattttat agttatgctc tttcacatta cgaataacta cttcagcaat actactagat
     1081 gtaactataa taagtgattt gttcccctca tttttcattt tatgtttcaa gtaatgtttc
     1141 cataagaggc gagttatata tgtaattaca acgtataaac ctcccatcaa gaaaagaacg
     1201 actctagaat aatcatttcc accctgaaca ctaatgagat acaaacttcc taacagcacc
     1261 aacataatcg cttgctttac agaagaagca aattctatat agtaccctct ttttaataca
     1321 cctctatatg cttcaaacat gaaaataact aaaagatctg ccagttcaat gaagactgcc
     1381 atgttttgat acattggaat cacataagga ttccaatcta cctggcgtaa gacatacgat
     1441 agataaaaag caagctggat acacaataaa tctaaaataa taaaatcaat atgtttaacc
     1501 catccccttg aacttttctt gtacattcct ttttactccc ctctttttaa taaaataaat
     1561 tgtattactt gttttctctc ctaaatatct ttctgaagga ttattgactc gacaaattgg
     1621 cttgtggata ttcttatata tagaacatcc gtaagccaac ttaaaactcc ttaacaaaat
     1681 ttttttactc atattgtaga tcaaaacaag tatccaagaa atcatatttt atttcttttt
     1741 tctgcttact caacaacagc taaaagaaaa atcatcataa acaaattcaa ctaagccaac
     1801 gatccccttg tgaaatcaag ttttttcttg atttacgaat aaagagctaa aataaaccaa
     1861 acttcttctt ttttacctct tcatagactg gataactgat ttcatccccg ttgatcagat
     1921 cctttgcgac ttgtttcatc tgtactactt tttccttacc aaagtccttt gcaatctgct
     1981 catagcactc ttttaagtaa aaggtgcgct tagagacccc atgggcatca gaagcagcca
     2041 tctgcacgag attatgtttg accatcgttt tggctgtttt ctggatgtct ttgccaaata
     2101 ccccaacata acttggcgca gtgagttggg ccaaacatcc catatctagg aacggaagca
     2161 aatgatttgg gtcttcgcga aaatgcacgt tgcgttctgg atggacgatt actggtgtct
     2221 ttcctagttc taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg
     2281 gaaattcgat cagaagatat gtatcatcta aatctgtaaa taatatttca tctcggtgga
     2341 tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca
     2401 actgtctttt ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt
     2461 cattgctgta ttttccatta ttgtggtgtg gtgtgcataa aatatgcgtg atgccttgct
     2521 gaatcgcttt ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc
     2581 ctggtaggat atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt
     2641 aatttccgta gtaataacca gcatctttgc tatgttctgc tccgttgtac acaacaccta
     2701 agactcgtgc ttgtaccatc tccagcagtt cttttgcttt agtcaaggat tctttacgtg
     2761 acatattttc acgtacgacc aataaagtgc catccgtttt taaagacatg atctgtgcat
     2821 ctgttacggc tactactggt ggcatatcga atatcaccac gtcaaataac tgtcttgctt
     2881 cggataggat ctgatccatt cttggcgagc ttaacaattc tgatgggttg ggtggcttag
     2941 gtccactagg caaaatcgac aagttctcga ctggcgttcg ttggatcaca tctgctacac
     3001 ttcctgatga actaagtgct gtacttaatc cactggcatt gttcagctgg aatgtcttat
     3061 agacgacggg tttacgtaaa tctgcatcga ctagtagaac tcgttgacct gatttagcaa
     3121 aaacgactgc aatgtttgct gcagtggtgg atttcccttc tccaggacct gatgaagtca
     3181 cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg
     3241 tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat
     3301 gagtttgtgc tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga
     3361 gcggcttctg cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctgt
     3421 tcctttttta cggataccag cgttcaattc tttcgctgac atttctggaa ctgttcctaa
     3481 gattgggaat cctaatgtat ctgtcacata tttttcatct ttcactgtcc gatcgagtaa
     3541 ttccaataag aatgccagac ctacacctac catcattcct aacattaagc cagtcattaa
     3601 attcaattta ttatttggcg aaacaggtgt cgtattggca actgcacctg aaatgatcga
     3661 gattttatca acattcatca catcttttgc gttctcttgg aaaacttgtg aagttgtgtt
     3721 agcgatctgt tgagcagcca ctgcattcgt acttattgcc tgaatcgaga acatttgtga
     3781 attttctgcc tgattaactg agatggcatc tttgatttgt tcagcagtca ttttcacatt
     3841 gtcttctgtt tctaaacgat ctttgacttc attgagcact aaatcactga cgatcatatc
     3901 tttatacgta ttgatcattt gcaggttcgt attgacatca ttgacatttg cagtgtctga
     3961 ttgtggcagt gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa
     4021 tgttacgatt cctgagatgg ccaagccagc aaacattgag atgatgatta ttaccaagtg
     4081 tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact
     4141 ttcctattta ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc
     4201 ttgatcactg actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg
     4261 atcttgctta atcgtaccaa atgcctcccg ataatctaaa gcaatttttt tcatttcatc
     4321 aaagttcatg tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct
     4381 gacaccgttt agactcaata cttttttcgc tacgccttct acgatctttc tttggcgccc
     4441 ttgtctaccg taatcaccat ttgggtcttc ataacgcata cgagaataag ataatgcctg
     4501 atccccgttc aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag
     4561 gtcattattg acttcgatac caccaactgc atcaacgagt tcctttagtc ctttcatatt
     4621 gatagagaca tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat
     4681 ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat
     4741 gtcgacatac gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac
     4801 tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc
     4861 aatccccatc aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg
     4921 ttctccttgg gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag
     4981 atataatttt gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat
     5041 aatcaaaaat atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt
     5101 ttgctaacct tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat
     5161 ttgctgagca acagtttgtg cttttgttgc gtatgcggct cttatttttt cattccggat
     5221 ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa gtagcggtag ctgttacttg
     5281 accgtctttc agcatcgtat cgaatagctg atctagttgc gtgacttgtg caatttgggt
     5341 atcagcgaat cctagatatt gcataacgac tgttttccaa gcactatcct taaaaaagct
     5401 catattttca cgtacatggg cgacatctgc acttgccaac ttttctttga tgatgacatc
     5461 atccacagct tgctgccagt ttgatacgtt tttagtaaat aatttattga cagccgtctg
     5521 gatcttcagt tttgattctg catcctctaa ctgcgttaag actgctttct tttcttcttg
     5581 gatcgcagca gcttctttag gtaattctga ttccttgatg tcataatcat ctgccgaaag
     5641 tttgatagaa gatagtttat gtgacaaatt tgaaactgtt tcttctgtta tttcaggatt
     5701 taggaaaatt ttttcttttg tactgtacag cttatctact tcttttgata aagaggtcac
     5761 ggtgtctttt tgattggtga tggcagattc tgcgtaattg atccgttgat gatattgccg
     5821 ctgattattg agatggtatc caaacaagca aatcacaata gcgcctgtgc ctaccgccac
     5881 agtagccaat aacttgacga acggcttttc catagattca ctcctaaata ttgatgatag
     5941 ataagtaaag atgtgattca ttcacatcag aatgagcgct ttttatttta aaagcataat
     6001 taaactaata tattcataga aatatttata aacaggtttt ccctatataa tcataccaca
     6061 tacagttttt ttgactaggg aaattcctaa tttaaaagta aataaaacag taacaagatt
     6121 ataattaatc gttttcattt ttatttcctc acttttcatg agcgttttga aaattcgaac
     6181 aaagaataaa at
//
LOCUS       DAYYNG010000155         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000155 DAYYNG010000000
VERSION     DAYYNG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.157"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHBFA_002488"
     CDS             725..811
                     /locus_tag="ACHBFA_002488"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9395908.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHBFA_002489"
     CDS             complement(932..1621)
                     /locus_tag="ACHBFA_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395909.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHBFA_002490"
     CDS             complement(1628..1906)
                     /locus_tag="ACHBFA_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395910.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHBFA_002491"
     CDS             complement(1866..2780)
                     /locus_tag="ACHBFA_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9395911.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHBFA_002492"
     CDS             complement(2762..3142)
                     /locus_tag="ACHBFA_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9395912.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHBFA_002493"
     CDS             complement(3592..3879)
                     /locus_tag="ACHBFA_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9395913.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHBFA_002494"
     CDS             complement(3899..4123)
                     /locus_tag="ACHBFA_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9395914.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHBFA_002495"
     CDS             complement(4578..4748)
                     /locus_tag="ACHBFA_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395915.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHBFA_002496"
     CDS             complement(4763..5296)
                     /locus_tag="ACHBFA_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9395916.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHBFA_002497"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHBFA_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9395917.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYNG010000156         6138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000156 DAYYNG010000000
VERSION     DAYYNG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.158"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(350..835)
                     /locus_tag="ACHBFA_002498"
     CDS             complement(350..835)
                     /locus_tag="ACHBFA_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395918.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(974..1189)
                     /locus_tag="ACHBFA_002499"
     CDS             complement(974..1189)
                     /locus_tag="ACHBFA_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM9395919.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1413..2213
                     /locus_tag="ACHBFA_002500"
     CDS             1413..2213
                     /locus_tag="ACHBFA_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9395920.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2232..4100
                     /locus_tag="ACHBFA_002501"
     CDS             2232..4100
                     /locus_tag="ACHBFA_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9395921.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4093..4584
                     /locus_tag="ACHBFA_002502"
     CDS             4093..4584
                     /locus_tag="ACHBFA_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM9395922.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4572..5126
                     /locus_tag="ACHBFA_002503"
     CDS             4572..5126
                     /locus_tag="ACHBFA_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM9395923.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5144..>6138
                     /locus_tag="ACHBFA_002504"
     CDS             5144..>6138
                     /locus_tag="ACHBFA_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM9395924.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2172 a          974 c         1227 g         1765 t
ORIGIN      
        1 taatacgata caaaggctat ctttctaatt tgaaaaaggg atcattaaaa taataagaag
       61 aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca aatatagaaa aattgaatct
      121 agaaaaccgc ccgacaataa atttcattaa ctcagttgac cgaaaatgaa gtagatacca
      181 aaaatttatc aagagagcaa gtagagaagt gagtatattc ataagagaaa tagctgtaac
      241 gaattattag gttaatgcaa atgcttattt atactccgta taactttctc agaaaaaatg
      301 ataccaataa tgacttgcta gtactgtaaa tccgtataat atcatctaat caaaatggtg
      361 ttccatttgc atctgtcatc gtaatcgatc cttcgggtga cactcgataa aaaccaacgc
      421 ttccagatcc tccttgggaa cggatttgtt tgctggaagc tgtgaacaaa taatctcgat
      481 caattttttg ataaaagcta ataacaatat cgtcactttg taattcggaa cggtgggcaa
      541 taagaaatag aagtgctttg tcagagtttt ctaatggaag agaaggtaca gtgctttctt
      601 gcatttcaga tgttgaagaa atgctaaacg actgaatact tgattggcta tgttgcgaat
      661 ctacattacc agtagaagta ttagtaatgg aagtagtaga agtactttta gttgaaccaa
      721 cctcagttgt tgaaatggct gctgaagatt ctttaacaga attctttgtt ttatatccac
      781 aggcagaaaa aagtattaca gttcccaaca agcctgcgta aatcccttta tgcatgatag
      841 ctcctcctaa aatagattat ctttattcag tatatcactt aaacaaagaa aagcagatgg
      901 tttaaaatga aaaaaccttc ccaataaagg gaaggttcat gaagataata acttttttaa
      961 taattatagc tttctaatat ctttcatcaa cttttctagt aatcattggg aaaaatttca
     1021 agcagtagat cgcacaaata cctacgacga tataaatgat cttcgcaaaa atagttgttg
     1081 aaccaccagc aatcatagct actaaatcaa gttcgaacaa gcctaccaac agccagttca
     1141 accctcccac aatcaataaa gttaatgcaa tagcatctaa agttttcatt agaattctcc
     1201 tttcttaaat gataaaacaa ttatatatca aaaagagaaa cattgcttat ataacgctca
     1261 ggaattttag caatctatgc aaaaagatga aaattttttt tgctcttcca ttagtacaca
     1321 ttttttagct gaagctgtaa atttcatgtc ttaatttttt ttagtgagct tttatactgg
     1381 atttgtaaga aaaaagaagg agaggatcac atatgtcgga ttggttggat attgcaggaa
     1441 aagtagtaat tgtaactgga ggttcctcag gaattggaag aagtatcgtc gaaaatttgt
     1501 tgaagcagaa tgcacaagta gcgaattttg atgtaactga atgccgtata cagcatgaaa
     1561 acctactatc tttaaaagtc gatgtgtctt ctaaaacgga catagaagaa gggatatata
     1621 aagtaatgaa acatttcaag acgatcgatg gtctagtcaa taatgcagga ataaatattc
     1681 cttctcttct aatagataga aatcatccaa aaagcaagta tgaattatca gaacaagttt
     1741 ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt gatgagccaa gcagttggaa
     1801 gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct ttcttctgaa tcaggattgg
     1861 aaggatcaga aggacagagc tgttatgctg ctacaaaagc agcgatgaac agttttactc
     1921 gttcttgggc aaaagagtta ggaaagagga atgttcgtgt ggtaggtgtt gcaccaggga
     1981 tactggaaga aaccggtttg agaacacaag aatatgaaga agctctttct tatacgagag
     2041 gtataagcgt cgaacagctg agaaacggtt acagtcgaac aagtacgatc cctctaggaa
     2101 ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta ttatctttca gaaagatcaa
     2161 gttatatcac tggagtcacg actaatattt caggaggtaa aacaagagga taaaggatag
     2221 gggggacgca cgtgacgcta gtaaacagat ggtaccaatt attgaaatta ctagtcactc
     2281 acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa aaccagtaat caaaccataa
     2341 aaaaagatat tggggagttg aatgaagaaa ttgccggtat tgcaaaaatc atccaaaaaa
     2401 acaaacattt tgaacttgaa atttatgatt ttgatttttt tgatgagatc atgcaaggaa
     2461 aattgaaaac agccagtgat ttcaattcgg catccaaaag aatcgcttat ttactaaata
     2521 ggctgatcga aacagaggat ttcttacgta tggatgattt gtctgaagaa gtcggtgtca
     2581 gcagaggaac agtggtcaag gatctaaaga cattaaaaga gatgatcaat gattttgatg
     2641 taaagattac aggcacacca aataaaggac taagaataat aggagatgag ctagaattac
     2701 gactactttt attttatttt gtttctccct acttttcaga gacgaaaaca gaacagtttc
     2761 tagaagaaaa acaagcaatc aaacattttc agaagaagac gaatgctagt aaacaagaaa
     2821 tcaccctttt aacgaaagtt atcggtattt ctttgaatag aatctctagt aactatctgt
     2881 tgaagaagcc catcaatcgt tacagtgggt gttttgaata tatccaagaa ttcaatgaac
     2941 tgatcaatca gttggaagaa atctatgaac taactttgag ccaatatgaa aaagatttta
     3001 tcagttttcc tttgaacatc atcaacacaa gtataacgat gataaggaaa gctaactctg
     3061 atttgcgctt atatttcgac cccatgatga tgcgtattcg acgattagat ctcattgatc
     3121 tggatgagtc cttcttatat caagagatga aacagcacct ctctttattg ataaatcggg
     3181 taatttttcg ttatcgattg actgatttat tttatggtga gatagaaaaa aagtatcctt
     3241 tttcttacca aatcgctagc gaatgcatac aagcattaga agctatcttg caacataaga
     3301 tctctaaagt agagatcagc tacctaacgt tgtatttgga gttagcattt cgatatcaaa
     3361 aagagcattt ttcctctaaa aaagtagcca ttgttcatac gacaggaaaa ggaacagcat
     3421 taatgatcca aagacaaatc aaaaggatat tggggaggga tatacaaata acacatgttt
     3481 cagaagaaca atatgaaaaa gtagatctaa acaaatattt cgctgttttt acaacgattc
     3541 cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt gactaatctt tttgatgact
     3601 cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg attagccggg tttcattcta
     3661 ctgttttttt ctatcaaata tggaaagaaa aaaagacgta tcaagaatat ttaaacaaat
     3721 tagtcgctcc tttgatcaga ggctattatg tggatgaaga atttgaaaaa aggatcttga
     3781 aaagagaata tcagcaaagc acatttgtta acaaagaatt tgcttttcct cacacaacca
     3841 accagctatc tcaacacatt gttttatcag tagggctaat aactgatcaa ggtttagcag
     3901 aaacaggagg aaatggagtt atttttttat ttgccatacc tgaagaaatg acggctgaag
     3961 ttgagaatga attatttgag ctatacgata tcctatttga actcataaaa atgggaatag
     4021 atgagtttat tgaattagca ccgacacaag aatcattttt aaaactaatc aaaagtaaag
     4081 gagagagact tcatgaatga tatgtatcta ttcatagttt tcgcagcttc agcttatgtg
     4141 ctgcaagcag ttatcagttg gaagcaacta aagcatttca atcaaacata tagcgaactg
     4201 cgaagaaaag gaaaagtagc gattggtaaa cgacctggga aaatccgttc aggcacgatt
     4261 gttatgctag ctatagacga tcatgatcaa gttttagaag tcagaaaaat gcaaggggtt
     4321 acatctttgg caaaatttca ttcattacct gtctatgcgg gtgaagatat ccattacatc
     4381 gatcgttacc atccctcagt tcgagcggaa gatcgtttga cacaagcagc tttagaaaat
     4441 gccagagaag tatacttacg aatagaggca ggaaatgaaa aaagtgaaca accttcattg
     4501 ccttttattg ctatgctcaa taattttcgg atatggaaaa gtacgatcaa agaaaaaagg
     4561 agtgacaaac gatggatatc ataacaaaat ttgctgaggg atttatgagg ctgttccaaa
     4621 caggtgcaga aacattcata agctggatga caggaatcgt accagttgtc ttgatgctga
     4681 tggttgcaat gaatacgctg attgcttttt taggtgaaga ccgagtaacg aaagtagcca
     4741 aattatcagc gaaaaatccg cttactcgtt accttgtgtt accgttctta tctgctttta
     4801 tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct tccagaatac tacaaaccaa
     4861 gctattatgc tgctcaagct caattttgtc atacgagcaa tggtgtattc ccccatatta
     4921 atcctggaga attgtttgta tggatgggga ttgctcaagg gatcgaaacc ttagggctaa
     4981 attcgatgga gctggctatc cgatacttgc ttgtgggact gctgatgaac tttattggtg
     5041 gttggataac tgattttact actggatttg tatgcagaca gcaaggaatc gttcttagca
     5101 aaaaagtaga gctttcagtt gattagagaa aggagagttt taaatgtatg gtgatattaa
     5161 gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt attaggccga caaaagataa
     5221 acacaagttc atctatgtca caggtggagg tgagaggcca atgatcgtag ataaaataga
     5281 agaactgaca ggtatggagt ctatcaatgg gttcaaaaca tcaatacctg atagtgaaat
     5341 cgcgcttgca atcgttgact gtggagggac tcttagatgt ggaatttatc ctaaaaaagg
     5401 gataccaacc atcaatatcg ttcccacagg aaaaagtggc ccattagcac aatatatcac
     5461 tgaagatatt tatgtttcag cagtagggtt agaacaaata cagccactaa tggaaactga
     5521 tgataacaaa gaaacagtaa atactgcatc tccagataaa aagtacaaat atgatacttc
     5581 taaaaagata accgagcaaa aagcagagca gcaaaactgg atagcaaaaa ttggaatggg
     5641 cgcgggaaaa gtagttgcta cattcaatca atcagcaaga gaagcagtag atacgatgat
     5701 ccggacagtg atccctttta tggctttcgt ttcccttttg attggtgtga tacaaggttc
     5761 tgggatcggt aatgtatttg ctaacattat ggcaccttta gcaggaaatg cagttggatt
     5821 aacaatcatt ggctttattt gttcactgcc atttttatct cctttgcttg gtccaggggc
     5881 agttattagc caagtgattg gaacattgat cggtgtagag attgggaaag gaaatattcc
     5941 tccccaatta gcattaccgg cactatttgc tatcaatact caaaatgcat gtgattttat
     6001 tccagtcggt ctaggtttgg aagaagcaaa agctgagaca gtagaagtag gtgtaccatc
     6061 agttctttat tcaagatttc ttaacggcat cccacgagtt atcattgctt gggtagcaag
     6121 ttttggatta tatagctg
//
LOCUS       DAYYNG010000157         5878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000157 DAYYNG010000000
VERSION     DAYYNG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.159"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..509
                     /gene="rpsT"
                     /locus_tag="ACHBFA_002505"
     CDS             255..509
                     /gene="rpsT"
                     /locus_tag="ACHBFA_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9395925.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(598..1644)
                     /gene="holA"
                     /locus_tag="ACHBFA_002506"
     CDS             complement(598..1644)
                     /gene="holA"
                     /locus_tag="ACHBFA_002506"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9395926.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(1682..3955)
                     /locus_tag="ACHBFA_002507"
     CDS             complement(1682..3955)
                     /locus_tag="ACHBFA_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9395927.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(3948..4487)
                     /locus_tag="ACHBFA_002508"
     CDS             complement(3948..4487)
                     /locus_tag="ACHBFA_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM9395928.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(4623..4841)
                     /locus_tag="ACHBFA_002509"
     CDS             complement(4623..4841)
                     /locus_tag="ACHBFA_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM9395929.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(4838..5083)
                     /locus_tag="ACHBFA_002510"
     CDS             complement(4838..5083)
                     /locus_tag="ACHBFA_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9395930.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(5199..5876)
                     /locus_tag="ACHBFA_002511"
     CDS             complement(5199..5876)
                     /locus_tag="ACHBFA_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9395931.1"
                     /translation="MIEKISKKILASPKLIIWMSMIVLIFCIASGFLLSNLLFYRETE
                     ETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGSVKEPGIYSFSSEERVYD
                     VLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEHLDQLASSEGKQPTADSE
                     PPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKVEDLQEISGIGEKTVEKL
                     KNFVTIK"
BASE COUNT         1816 a         1205 c          963 g         1894 t
ORIGIN      
        1 ccattcaaac tttgtttaaa tagataaata aaaatctagt ttggtttgac gctattgcct
       61 cccacaatag cgtctttttg atttttttca gcttgtacta tccgttttta ctgaaaaaaa
      121 atcctaataa aagactgtta aggaaaaaaa cttgtcatgt tcatattgca aagcacgctg
      181 cctttaggta taataatgaa tgttgaatta tgcggtaaca cgcgtaagaa cttaagttat
      241 cggaggtgaa actaatgcca aacattgaat ctgcaatcaa acgtgttcgt acaagcgaaa
      301 acgctaatgt gaaaaattca tctcaaacaa gcgctatgcg tactgcaatc aaaaaatttg
      361 aagatgctgt agcttcaggt gctgacaacg tagatgcgtt atataaagaa gctgttaaag
      421 ctattgatat ggctgaatca aaaggtctaa tccacaaaaa caaagctaac cgcgacaaat
      481 ctcgtttaag caaaaaaatc gctaaataaa agaatcggct tcggccgatt tttttatttg
      541 tcaaaaaaag gacaaaaaca aacgaccttc ccgcatgctt tgccctcgtc tttcttctta
      601 tcgatttctt aacaactgcc ctgtgcgcaa aacgaatagt tgaaaactta attctttttc
      661 gatttgtccc gttttgattt gataatccat ctcaactaat tgatcaaaca actgtaccag
      721 caaatgttca tcgaattttt ttacttcctg catggcaagt tttacacgat atggatggat
      781 tttcaatgtt tcagcaatat ttgcctgctg ataaccaatc tttacaagaa attttgtctg
      841 taaaaataaa cgtaattgag aaagcaaaat cgcattaatt ttgattgttt cttccccttg
      901 cataactaaa tcttcgtata aacgcaaagc ttgttccgtc ttccctttta aaatatattg
      961 ggtcatgtca aaaatattat gttctaacgt tttaggaact aattcttcta cttctcttac
     1021 cgtgatttgc tgttgatttt gtccataaag gaccaatttc tgtaactctc tcatcatttt
     1081 ggataaatct aagtcggtca acctcaagaa taaatcaatt gcacttcgtt ccattttgat
     1141 tgaggaattc tctaaggtat tcatcagata ttgacgtact tctttttcat tcaactgttg
     1201 gacattgatg ataattgctg tttttttcaa ttgtttcgtt acttttttcc gttcatccaa
     1261 cttttcatat ggcgcaaaga atactaatac agtcgattct aatggttctt tcaaataagc
     1321 cgtcaataaa tcagtatttt gttcgattcc attgtttact ttttcacctg ttaaaaaata
     1381 aggattctct acgaatacta accgttgatc tccgaaaaac ggcaacgtct ctgcttcagc
     1441 tactacctca tctaaacttg cattttccat gtcaaaactt aaaaaattta agtcatcttc
     1501 gccctctaat tggatttttt tgacaatttc tgtccgaact tgatcttgga gaaacttttc
     1561 tgttcctaaa atcaagtaac aagatgcaag tttttcttta cgtatttttt gtaactctgc
     1621 ttgtatactc aaattactca ccactttcaa aaattatcct accatgagca gatgagaatt
     1681 actagtctat cattgtttcc actagcccat gaggatgaaa aactggatgc cagcgatagg
     1741 tgatcattcc ttgttgatcc gttcgccatg tttttatctg attttcttct agtgtctcca
     1801 atacttcgct gtgcgggtga ccataacgat tatttacgcc gcaggatata atcccatgtt
     1861 ttgcttcaat atgggaaaga aaatctgtgt tacttgacgt acggctaccg tgatgaccta
     1921 atttgactac atcggctttc agattaggat aaatcaaaag caattgtttt tccattgctt
     1981 gattcaaatc tcctaaaaat aaaaagcgcc tattgttata ttccatccat agaccaatcg
     2041 aatcttcatt tcttccctct cccttttcaa aaggagtcaa tacataaaga tcataataac
     2101 cgctgatcct gtccccatgt ttgatttcaa tgatctcagt accatttttt tgagcttcag
     2161 ataaatgcct ttgtaatggc tcatttcgtc cagcgcccca accgatataa atttttttga
     2221 tagtaaaatg ctgagataac gtgggtaatt ctcccacatg atccgtatcg tcgtgtgtca
     2281 agatgagttt atcgattaca cttatcccct gaccttttag atatggaacg atatttcttt
     2341 cagcaggagg acgtatcata ccttgcgccc acttttctct ttcaaagtgt aactgacctc
     2401 cagtatcgat caaaatcgtt tcttttcgaa aaggactttg caaaaagata ctgtctcctt
     2461 gaccgacatc gataaaagcg atcgttgaag aaaagcgtaa aaatggcaaa gtcaaaaaaa
     2521 taaaagaaaa aagaccaaca taaaacaagt gtttcacagg ttgttctttc aacctatcca
     2581 tacaaatcag taatccgaaa atatacatcc cagatagcac aaggggcaaa cgtcctataa
     2641 tgagagacga ccctgaagaa aaatcgatta ttcgctcaaa taagattaat ccctcttcta
     2701 tcagcaaaag taaagtttcc ggcagccagc gattgaagag tgtgagaaat aacacaccag
     2761 gaagaaaaag aaacttgaat accggtgcaa tcagtaatgt aaaaagacta cctaaaatag
     2821 gccactcata aaaatgccat acaatcaacg gcatcgtgat catagtaaac aggacgtgag
     2881 aggccgtttt tttcaaccaa tctccatcaa aagaaatcag gataaacaaa aaactcatga
     2941 aaaaactaag ctgtccaccc gcatgaaaaa gaaatcgagg atcaaatagt aaaagaagcc
     3001 aaaagacaac tgcaaataag tccatggtgg aataatgctt attcagcaat ttgactagca
     3061 atcgcaataa aaacagcaaa ctagcacgca aaacagaaat agaacttcct gcaatagaaa
     3121 cataacagat agttagtaga ctcaacggaa taatactgac gtgtagtggc catccgatac
     3181 gtcgaaataa ataatacagc caccctaaat aaaattggat gtgtagaccc gatagactga
     3241 acagatgcag caaaccactc tccctaaaaa tttcttctgg ttctctgaat cctgcatctt
     3301 tatagcccca taataaagaa ttaatatata cacttgtttt ttgcggaaat tcagattgga
     3361 caaaggagat aaaagatcct ctttttctct gaagccagtt tcgccagtga ttttgcatgg
     3421 tgatctgttc aatcttttga atttggaacc gtccagaaat tctatggttc ctgaagtaag
     3481 cttcttggtc aaaattataa agattccgct tttttgctgg agagatgaac tcgccttcag
     3541 cagtcaattc tattcccttt cctcggaagt taagccatgt tttcaattct gcttctgtgt
     3601 ttacttgata agaaagctgg agcttttgtt tattctctga taaccttcct tccattgtca
     3661 aaaaattacc atttgcacga atcgtatctg taaatacagt aatttgcgca gatgttgttt
     3721 tttcctctaa aaatgaatac tcttgtagcc tataagaaat ggataccgtt atgctacaca
     3781 aaatacacaa gtacgcgata aaacgtcctt cttttagcga aaaccataaa aaaattccgg
     3841 caagaagcaa acgataaccc caattgtcag tgtaatacaa caaacagata atcatcgacg
     3901 taattgccgg aaaaatcaac cgatttcgaa catgcctttc taaaacgcta gacatgtttt
     3961 cctgttaatt cattttgaag ttttgaaaaa tgctgtgagt ctaaagcaac ttggtgtaca
     4021 gatacaccta attgttcgat caacgcttgg gcataaggat catttcgata gtcatggaga
     4081 taattgatcc ctttcacacc agcttgcaac aaagcctttg tacaggccaa acatggaaaa
     4141 tgtgtgacat agatttctga accttccgtc gggataccaa gttttgcaca ctgtaggagt
     4201 gcattcatct ctgcatggat cgttcgcaag cagtgtccat ccacgacgta acacccttca
     4261 tctatgcagt ggacatctcc actgactgct ccattatagc cgccagcaat gatccttttt
     4321 tctcgaacga gtgtcgctcc tacttctaaa cgtgtacacg tactacgtaa agataacagt
     4381 actgcctgag ccataaagta ctggtcccaa ggaatgcgtt cattatgtga cttatcagta
     4441 actttttctt tattcgtatc gcatgctttt gaagattttt ctaacatgtt ttatattcct
     4501 ccgttttttc aagttgatta aaaagtactc attgcttaaa caaaatattc tttaaatata
     4561 acgaatcatc ctgtctgaca aaagatccta cccttcactg ccgaaatgaa gtccgtcatc
     4621 cattagccca ttataaaaat tcttcgaatc catttgttca agaaattccg tataaaaatc
     4681 gatacttcac aatcatggcg ttttgctact tctgtgagaa cagagatata ttctttgatc
     4741 aacttgttat tccgatattt ttgttttgtc tcatcgactg ctggtggcgt gataaagaaa
     4801 gcatgatcag aactatagtc ttttttcagc tgaactatca tccactgcag attttttggt
     4861 acgtttcttt agaaagttgt ttatgctctg ctagatcatt cgtcccaatg agaataaata
     4921 ctttatctgc tgtatagcta agtacatctt tggcaaagcg ttctcgtaga gcttgactgt
     4981 tattgccgct gacagccgta ttcataatat gaatcccaga tattttattt tggagataga
     5041 cattcagcat tggttttttt aatgtttctt tgcgaacaat caagctattt cctatccaaa
     5101 aatactacct cctcgtcctg ccaagctctt agaattggat tattcttagt atccattagc
     5161 gtccatttat tgctcgtgcc agtcgatttt gaatcattct atttaattgt aacgaagttt
     5221 tttagttttt caactgtctt ttcaccgata ccagaaatct cctgcaggtc ttccactttc
     5281 tggaaggatc cgttttcttc tctaaatcga atgatttctt gcgctttgac tgatccgatc
     5341 ccaggaatct gctgaagttc tgatggagtg gctgtattga tatttatttt gggaggctcg
     5401 gaatctgctg taggctgttt tccttccgag gatgccaact gatcaagatg ttctggcagc
     5461 tcttcaccta cagcagggat atataggact tgctgatctt ctatcttcgc agaaaagttg
     5521 attcgatcgc tatccgcttc ttctaataat ccacctgcac gctttagtac atcatagacc
     5581 cgttcttctg aagaaaagga atagatacca ggctccttca cacttccttt gatatcggtg
     5641 tacatgacct ttggacgagc atcctcttct acatcgtttc cttcagctga tggatcttct
     5701 ccactggaac taaacgaaga aagtatttct tttgcagcag tctcttctgt ttctctataa
     5761 aatagcaaat ttgataataa aaaaccactt gcgatacaaa agataagaac tatcatagac
     5821 atccaaatga tcaatttggg tgaagctagt attttcttgc tgattttttc tatcattg
//
LOCUS       DAYYNG010000158         5749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000158 DAYYNG010000000
VERSION     DAYYNG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.160"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACHBFA_002512"
     CDS             complement(<1..1128)
                     /locus_tag="ACHBFA_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM9395932.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACHBFA_002513"
     CDS             complement(1164..1874)
                     /locus_tag="ACHBFA_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395933.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="ACHBFA_002514"
     CDS             complement(1942..2757)
                     /locus_tag="ACHBFA_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM9395934.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACHBFA_002515"
     CDS             complement(2768..3736)
                     /locus_tag="ACHBFA_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9395935.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="ACHBFA_002516"
     CDS             4499..>5749
                     /locus_tag="ACHBFA_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9395936.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1183 c          915 g         1879 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DAYYNG010000159         5708 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000159 DAYYNG010000000
VERSION     DAYYNG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.161"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACHBFA_002517"
     CDS             <1..377
                     /locus_tag="ACHBFA_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9395937.1"
                     /translation="YSLFKTLQQNLSLYGKRGTMENFIKEAKGGFYFDKTDSPRFLEN
                     HVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHSIRLILLKVAGKLVQTGRQVYLKLSS
                     YHVYQTEFYKVFERLRRSKQWI"
     gene            529..2055
                     /locus_tag="ACHBFA_002518"
     CDS             529..2055
                     /locus_tag="ACHBFA_002518"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFM9395938.1"
                     /translation="MAICSKKQVKFRAMNYSGFFEVEASIKVNQGSFKGVLSPTQRFK
                     SQEELLSFVSSKQAKIGNQEGIIADVQTKEKKTNSPSLFSLSSLQSKVNQLYKATASQ
                     TLKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMVQTEPRKRY
                     VDGSKVQEHHAIIPTKQVPTESALAKMDDLQRKIYALVVKTTVAMFLPDYLYEETKIQ
                     TKVADLLFQSIGKTPKQEGWKILFKQQTKEEKEDVQTLPLVIIGEHAEVDVKSAEKET
                     QPPKAFTEGTLLTAMKTANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEYIQV
                     IKNKLVVTEKGKLLCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFINNIKKFI
                     VHLLEAVPNDIEKLNFSDYQVQKEKEAEKSIVGKCPKCGNNIVLKKSFYGCSNYPECK
                     FTLAEHFRKKKLTKTNVKELLEGKETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK
                     "
     gene            2158..3054
                     /locus_tag="ACHBFA_002519"
     CDS             2158..3054
                     /locus_tag="ACHBFA_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9395939.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYKNVFFEMLERIIQLENEKE"
     gene            3118..3333
                     /locus_tag="ACHBFA_002520"
     CDS             3118..3333
                     /locus_tag="ACHBFA_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9395940.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            3350..3622
                     /locus_tag="ACHBFA_002521"
     CDS             3350..3622
                     /locus_tag="ACHBFA_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9395941.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            3624..4487
                     /locus_tag="ACHBFA_002522"
     CDS             3624..4487
                     /locus_tag="ACHBFA_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9395942.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNLIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTAYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGL"
     gene            4618..4884
                     /locus_tag="ACHBFA_002523"
                     /pseudo
     CDS             4618..4884
                     /locus_tag="ACHBFA_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            complement(4938..>5708)
                     /locus_tag="ACHBFA_002524"
     CDS             complement(4938..>5708)
                     /locus_tag="ACHBFA_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFM9395943.1"
                     /translation="MTKVLTEEVNAFKVRTLNDKYVAIFMDATYIPLKRQTVSKEAIY
                     IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDVLLFITDGLKGMKDTIH
                     QIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAVYQASSKEEANTFLGSMIEKW
                     QKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFNKQIKKYSRRKEQFQNEE
                     SLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         2166 a          910 c         1026 g         1606 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttctct ttacggcaag cgaggaacaa
       61 tggaaaattt catcaaagaa gcgaaaggtg gcttttactt cgataaaaca gatagcccgc
      121 gtttcttaga aaatcatgtc agaatgatga tcagtgtcct tgcatacaac ctcgttaatt
      181 ttttaaaaag cattggtttt gataaagtga accagggcat gacgattcat tccatccggc
      241 tgatattgct taaagtagcc ggtaaacttg ttcagactgg gagacaggtc tatctcaaac
      301 tgtcaagtta tcatgtgtat caaactgaat tttataaggt ttttgaacgc ctgcggcgat
      361 ccaaacaatg gatttagact agttatcaca caaatttttt aaccgattct ttttcaaggg
      421 attcgtctgc ccttaaatag acaaataatt tttattatga tcttcttccg taggcatcct
      481 gttcgaaaac acattatttt ggaagaaagt atctgcctaa tttaaaaatt ggcaatttgt
      541 tcaaaaaaac aggttaaatt tagagctatg aattattcag gttttttcga ggtggaagct
      601 agtataaaag taaaccaagg gtcgtttaag ggcgttctaa gccccacaca gcgttttaag
      661 agccaagagg aacttttatc ctttgtttct tctaaacaag ctaaaatagg caatcaagag
      721 gggataattg ctgatgttca aaccaaagag aagaaaacga atagtccgag tttgttttct
      781 ttaagtagtt tacaatcaaa agtcaatcag ctttataaag cgacagcaag ccaaacttta
      841 aaagctatgc aaggactgta tgaagcaaaa ttattgagtt atccaagaac agatacacca
      901 tttattacag agaacgaatt tgcttattta aaagcaaatt ttggcaaata tagcggtttt
      961 ttaggacttg atctcgaaat ggttcaaaca gagcctagaa agcgttatgt ggacggtagt
     1021 aaggtacagg aacaccacgc cattatccca acaaaacaag tacctaccga atctgcatta
     1081 gcaaaaatgg acgatttaca acgaaaaata tatgctttag tcgttaaaac gaccgttgcc
     1141 atgtttttac ctgattactt gtatgaagaa accaagatac aaacgaaagt agctgatttg
     1201 ctgtttcaat cgatcggaaa gacaccaaaa caagaaggtt ggaaaattct tttcaaacaa
     1261 caaaccaaag aagaaaaaga ggacgttcaa acgttaccct tggttatcat tggagaacat
     1321 gccgaggttg acgttaagag tgccgaaaaa gaaacacaac caccgaaagc ttttacagag
     1381 ggtacattat taactgctat gaaaaccgct aataaaacgg ttgatgatga agaagcaatc
     1441 aagattttac aagaagtcga ggggatcgga acagaagcga caagagcaag tattattgaa
     1501 gcgttaaaac aaaaagaata tatccaagtg attaagaata agcttgttgt aactgaaaaa
     1561 ggaaaattat tgtgccaggc agttgaaagt cagcaccttt taacgagtgc tgaaatgacg
     1621 gctaaatggg aaacgtattt aaaaaaaatc ggtaaaagag aaggcaatca agaaaacttt
     1681 attaataata tcaagaagtt tatcgttcat ttactggaag ctgtacctaa cgatatagaa
     1741 aaactaaatt tttctgatta ccaggtacag aaagaaaaag aagctgaaaa aagtattgta
     1801 ggaaaatgtc ctaagtgtgg taacaatatt gtattaaaaa aatcgtttta tggttgttca
     1861 aattatcctg aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa
     1921 acgaatgtaa aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa
     1981 gagaaaaagc cctacaatgc cgttgtaaaa atcggagaaa agggatatat tgattttatc
     2041 tctttctcaa aataaacata aaagcccttt aaacagggct tttatatatt aatcacaaat
     2101 cacttatcac aaatcacaag tgattaatca caaatcactt gtgatttatg attcctaatg
     2161 atacaatatt actatacaaa aaaagaatgg ggcgtagtta tggagaagga agaactaaaa
     2221 atacttgaag aattaagacg tattttaaac agtaaaaatg aagcaattgt tatcttaaac
     2281 aattatttta aaggtggtgt tggaaagtca aaattatcga ctatgtttgc ttacttgaca
     2341 gacaaattta atttaaaagt tttaatgatc gataaggact tacaagcaac attgacaaaa
     2401 gacttagcaa aaacatttaa ggtagaattg ccacgtgtca atttttatga aggactgaaa
     2461 aatggaaact tggcttcttc tattattcat ttgactgata atttagactt gattcctggc
     2521 acgtttgatt tgatgttact gccaaaatta actcgctcat ggacgtttga aaacgaaagt
     2581 agattgcttg ctactctttt agcaccttta aaaagtgact atgatctcat tattattgat
     2641 actgtaccaa cgccaagcgt ttatacaaat aatgcaatcg tggcgagtga ttacgttatg
     2701 atccctttac aagcagaaga agaaagtaca aacaacattc aaaactatat ttcctatttg
     2761 attgatttac aagaacaatt taaccctgga ctagatatga tcggttttgt tccttattta
     2821 gttgatacgg acagcgcaac gataaaatca aacctggaag aactgtataa gcaacataaa
     2881 gaagataact tggttttcca aaatattatc aagcgaagta ataaagtaag tacctggtct
     2941 aaaaacggca ttacagagca taaaggctat gacaaaaaag ttttatccat gtataagaac
     3001 gtattttttg aaatgcttga gcgaattatt caattagaaa acgaaaaaga atagaatcac
     3061 aaatcacaag tgattgttga tgataaaata agagtaagaa gaaatagaaa gaagtgagtg
     3121 attgtgggaa atttaggcgc acaaaaagca aaacgaaatg atacaccaat cagtgcaaaa
     3181 aaagatataa tgggagataa gacggttcgt gttcgtgctg acttgcacca tatcataaaa
     3241 atcgaaacag caaagaatgg cggaaacgta aaagaagtta tggatcaagc cttagaagaa
     3301 tatatacgga aatatttacc tgacaaactt taaaaggagt gaaaatgaaa tggcagttac
     3361 gtatgaaaaa acatttgaaa tagagatcat taacgaatta tcggcaagcg tttataatcg
     3421 agtattaaac tatgttttga accatgaatt aaataaaaat gactctcaat tattggaagt
     3481 caatttatta aaccaattaa agcttgcaaa acgtgtaaat ctttttgatt attctttaga
     3541 agaattacaa gccgttcatg agtattggcg gtcaatgaat cgttactcaa aacaagtttt
     3601 gaataaagag aaagtggctt aatatggcaa atatagtcaa ttttactgac aaacaatttg
     3661 agaatcgctt aaatgataat ttagaagaat tgattcaagg aaaaaaagcg gttgaatcgc
     3721 caaccgcttt tttacttggt gggcaaccag ggtcagggaa aaccagtttg cgatcagcaa
     3781 tttttgaaga aacacaaggg aatcttattg tcattgataa tgataccttt aaacaacagc
     3841 accctaattt tgatgaacta gtgaaacttt atgaaaaaga cgtagtaaaa cacgttaccg
     3901 cttattctaa tcgcatgaca gaagcgatca taagccgttt gagcgatcaa gggtataatt
     3961 tggtgatcga aggtacagga cgaacaacag acgttcctat tcaaaccgca acaatgcttc
     4021 aagccaaagg ttatgaaaca aaaatgtatg tcatggcagt acctaaaatc aactcatatt
     4081 taggaacaat tgaacgatat gaaaccatgt atgcagatga tccaatgaca gccagggcaa
     4141 caccaaaaca agcgcatgat attgttgtca aaaacttacc gaccaattta gaaacccttc
     4201 ataaaacggg cttatttagc gatataaggc tatataacag agaaggagta aaactctatt
     4261 caagtttgga aacaccttct attagtccaa aagaaacctt agaaaaagaa ttgaatcgta
     4321 aagtatcagg gaaagaaatt caaccgactt tagaacgaat agagcaaaaa atggttctaa
     4381 ataaacacca agagacacct gaatttaaag caattcaaca aaaattggaa agcttgcagc
     4441 cacctacacc accaataccc aaaacaccta aacttccagg actttaaacc ttaaaaaagg
     4501 aaaatagtag ttaccaaaaa acggtaacta ctcttttttt ataaaaacat ttcctcaaac
     4561 gcctaacaaa aatggttgtc aaaaagccaa aaatcaaaaa gaaaaggaat gataaaaatg
     4621 aaatacatta aaaatgttga aacattggaa gaattgaaaa aagcctacaa aaagttagct
     4681 tttaagttac atcccgattg tggcggaaat gaagaagaaa tgaaaatttt gaacaatgaa
     4741 tatgacgaac tgtttagcaa acttaaaaac actcataaga ataaagaagg taaaacttac
     4801 accaaagaaa caacagaaac acccgaacag tttaaggata ttattaataa attgttcaat
     4861 ctaaaaatgg acggcgtagc aatcggggag cgtcaataat tttgtgtaaa taactcgtcc
     4921 ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt cacttgttgg
     4981 aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat ggatactagg
     5041 aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta tttcttgatt
     5101 tgtttattga aagattcgat taaattagtt gagtaaatgc ttcggcggat accaggcgga
     5161 aactcataaa aagtcaataa atcttgattt tttatcagtg actgcgtcac tttaggataa
     5221 gttttctgcc acttctcaat catactccct aaaaaggtat ttgcctcttc ctttgagcta
     5281 gcttgataaa cagccttaaa atcatcacag atttcttttc gatctttgac acgtacttta
     5341 tgagcaatat tacgggaaac gtgaacacag caatgttggt atttagcttt aggataaatc
     5401 tggtgaatcg tatctttcat gccttttaag ccatcggtga taaacagtaa gacatcatga
     5461 acccctctgg agtaaatgtc ctgtaacaac tcattccaaa cgtaggtcga ttcagtcgga
     5521 gcaatcgcat aacttagtac ttctttagtg ccgtcttctc gtataccaat ggcaatatat
     5581 attgcttctt tggagacggt ttgccgtttt aaaggaatat aagtagcatc catgaaaata
     5641 gcgacatact tatcatttag agttctaact ttaaatgcat ttacttcttc agtcagcact
     5701 ttagtcat
//
LOCUS       DAYYNG010000160         5610 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000160 DAYYNG010000000
VERSION     DAYYNG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.162"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..767
                     /locus_tag="ACHBFA_002525"
     CDS             66..767
                     /locus_tag="ACHBFA_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9395944.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ACHBFA_002526"
     CDS             764..1885
                     /locus_tag="ACHBFA_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9395945.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ACHBFA_002527"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ACHBFA_002527"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9395946.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ACHBFA_002528"
     CDS             3264..4172
                     /locus_tag="ACHBFA_002528"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9395947.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ACHBFA_002529"
     CDS             4207..4479
                     /locus_tag="ACHBFA_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9395948.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ACHBFA_002530"
     CDS             4490..5536
                     /locus_tag="ACHBFA_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM9395949.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1821 a          972 c         1218 g         1599 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg
//
LOCUS       DAYYNG010000161         5585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000161 DAYYNG010000000
VERSION     DAYYNG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.163"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACHBFA_002531"
     CDS             complement(488..1297)
                     /locus_tag="ACHBFA_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9395950.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACHBFA_002532"
     CDS             complement(1297..2043)
                     /locus_tag="ACHBFA_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9395951.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACHBFA_002533"
     CDS             complement(2061..2528)
                     /locus_tag="ACHBFA_002533"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9395952.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACHBFA_002534"
     CDS             complement(2528..2938)
                     /locus_tag="ACHBFA_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9395953.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACHBFA_002535"
     CDS             complement(2949..3962)
                     /locus_tag="ACHBFA_002535"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9395954.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACHBFA_002536"
     CDS             4179..4913
                     /locus_tag="ACHBFA_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395955.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="ACHBFA_002537"
     CDS             4897..>5585
                     /locus_tag="ACHBFA_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9395956.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DAYYNG010000162         5540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000162 DAYYNG010000000
VERSION     DAYYNG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.164"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..1026
                     /gene="pfkB"
                     /locus_tag="ACHBFA_002538"
     CDS             91..1026
                     /gene="pfkB"
                     /locus_tag="ACHBFA_002538"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9395957.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            1023..2468
                     /locus_tag="ACHBFA_002539"
     CDS             1023..2468
                     /locus_tag="ACHBFA_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9395958.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            2496..2951
                     /locus_tag="ACHBFA_002540"
     CDS             2496..2951
                     /locus_tag="ACHBFA_002540"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9395959.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            2970..3944
                     /gene="lacD"
                     /locus_tag="ACHBFA_002541"
     CDS             2970..3944
                     /gene="lacD"
                     /locus_tag="ACHBFA_002541"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9395960.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(4055..4616)
                     /locus_tag="ACHBFA_002542"
                     /pseudo
     CDS             complement(4055..4616)
                     /locus_tag="ACHBFA_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            4760..5374
                     /locus_tag="ACHBFA_002543"
     CDS             4760..5374
                     /locus_tag="ACHBFA_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9395961.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         1766 a          891 c         1210 g         1673 t
ORIGIN      
        1 tgtaaaagtt ttcaatgaat ttagtcattt atgaaaacat ttacattcga tcttgataca
       61 taatgaatat gtaagaaagg aggagcgtaa atgtcagttt atacatgtac tttaaacttg
      121 gcaatcgacc tttttatcga aacagatgaa atgaaaccat cccaagtcaa tcgaacaaac
      181 gatgatgata tacaagctaa tggaaaaggt gtgaatgttt cattgatcat gaagcaatta
      241 ggtatcgata gtacagcttt aggatttaaa gcaggtttta ccgggaatta tattgaggat
      301 tatttgcatc aaaaagatat tagcactgat tttattagcg taccgggaat gactagaatc
      361 aacgttttta cacaagttaa taaaaccaat gaagaattca aattggttaa taaaggacct
      421 gagattcctc aggaacaaat caaaactttt ctaaatcaaa tacaaaagct gcaagctgac
      481 gattatttgt gtgtttctgg cagtttgccg cgaggggtct cacccgaaat tcttattgat
      541 atcagtcgta tttgttttga aaataaggtc catctgatta ttgattcaag ctatccggaa
      601 ataattgatt gtctgccgta tcagccgttc ttattaaaac caaacgagga ggaattgcaa
      661 tcttggtata atgtcgagct tagtgatcgt gaaggatata ttcattatgc tgaacgcttg
      721 attgccgatg gagcgaaaaa tgttctcctt tctctaggag gagagggagg aatattttta
      781 aataaggata tcgtttattc tgggaattct cctaaaggta aagtagtcaa tacagcgtgt
      841 gctggtgaca ctttgttggg aacatttctt gcaggatatt taaaaggtga acaggtagat
      901 attaatttga agaaaagttt agcagctgga agttcaaccg cttttcgtaa aggattaaca
      961 gatttttcgg atgttgcaga gttggaaaaa caaatcgaaa tcaaagaggg agcgagtaaa
     1021 aaatgacaac ttacaaaatt gttgccgcaa caggatgtcc aacaggaatt gcccacactt
     1081 atatggctca agaagcattg gagcaggcag ctaagcgcaa aggggtatct atcaaagttg
     1141 aaacccatgg tcaagtaggg atagagaatg aattaacacc agaagaaata aaaaacgctg
     1201 acgctgtaat cattgcagct gacaaagatg ttcaagcaga acgtttcgca ggtaagcgtg
     1261 taatcgatgt gcctgtcagc aaggggatta aagatgctga ccaattgatc gaagatgcgt
     1321 tggctggtaa aggttcaatc actgcagaaa acaaaacagt tgacgctgct ggtgagaaag
     1381 cggagatggg ggttgcagca ggcattggtc acagtattta taaaaatctc atgaacggtg
     1441 tttctcatat gctgccgttt gtagtggcag gtggggtaat tattgcaatt tcgtttgcca
     1501 tctggggtgt gtactctttt gatcccaatg atcctactta caatccgact gccgctatgt
     1561 taaagggtgt tggtgatgcg gcaatgggaa tgatggttcc tattttagca gcttatatcg
     1621 ctgaaggaat cgctaaacgt cctggtttgg ttgttgggtt tgttggcgga ctgttagcca
     1681 attctggcgg agccggtttc ttaggcggaa tttttgcagg tttcttagca ggctacttta
     1741 tcttactatt gcaaaaggtc atgaaaggct tgcctaaaca attggatggt ctgaaggcga
     1801 tcttcttata tccggtgatc ggtgtcttgt ttgttggtgt agtcatgtct ttattagcaa
     1861 atccaatgga agcagtcaac caaggaatga tgaatttctt ggcaggattc caaaattcta
     1921 atccattaat tttagggatt atcgttggct gtatgtcagc atttgatatg ggaggtccag
     1981 tcaataaagc ggcttatgtt acagggactg cgttgttggc acaaggtaac catacattta
     2041 tggcaggggt ttcggcagct tgtatcgcgc ccccattgat tactggattt gccgcactgt
     2101 tttttggaaa atatttcgac acgaatgatc gcaacgctgg tttagtcaac tttatcttgg
     2161 gctcaacgca cattaccgaa ggagcaattc catttgcggc taaagatccg ctgcgtgttc
     2221 tgccaactat gatgctaggt tcatcaattg ctgctgtact gacatatatg tttggtgtcc
     2281 aagttcccgc acctcatgga ggttttctag tattacctgt tgttactggt aaggttgcat
     2341 gggtagggtc gattttactt ggttcaatcg ttggcggagt aattttaggg ttgatccaaa
     2401 aacgtagtgt agaaaaacgt gaagcagaag cagctgctgt tgaaatgaaa gaagcaacca
     2461 acaactaaag gttattctag aaagaaggat ttcaaatgac tttgatcaaa acaaataaag
     2521 tttttatgaa taaaaatttt acgacgcaag atgaggtaat gaacttcctt gcagatcagt
     2581 caattgtctt gagcattagt gaagaccgag atgcagttta cgaaaaattg atggaaagag
     2641 aagaagaaag cacgactggt atgatggatg gttttgccat ccctcatgcc aaagatgcta
     2701 gtatcaagga tgccgatatt gtgattgttc ggttaaacgg taaggtcgat tggaaaagtt
     2761 tagatggaga aggaacggat tttgtaatcg ctctgtttat tccagattca gaggctggaa
     2821 caacccatct aaaattttta tctcaggtag ccagactttt gatgcacaaa gatgtgacag
     2881 aaggtctgaa acaagcccag tctgctgaag aaatagctga acttttaaac caaaaattag
     2941 aagaaaacta aaaaggaagt agtgaaatca tgcctttaac agaaaaaaag aaaacttata
     3001 ttcgtcaact aagtaatgaa agtggcatca tcagtgcgtt agcaatcgat caacgtggtg
     3061 ccttgaaaaa aatgatcggt aaatttcaaa ataaatcagc aaccgatgaa caaatcatca
     3121 atttcaaaaa aatcatttct tctgaattga ctccatatgc ttcagcaatc ttattagatc
     3181 cagaatatgg gttgccggca gccgaagttc gcgataaaaa tgctggtctg ttgttggcct
     3241 atgaaaaaac tggttacgat gcttcaactc caggacgttt accggatatt ttatctattt
     3301 ggtctgtaaa acgtctaaag gagcaagggg ccgatgcttg taagttcttg ttatactacg
     3361 atgtagatga atctgatgac atcaatgacc aaaagaaggc gtttatggag cggattggtt
     3421 ctgaatgtgt tgctgaagat ttgccatttt tcttagaatt ggtttcttat gatgccaacg
     3481 atcctgatac ttcttcgaag gaatatgcct tgaaaaaacc gcataaagta aatgaaatga
     3541 tgaaggaatt ttctaagcct cgctatggag ttgacgtttt gaaagtggaa gttcctgtta
     3601 atatgaacta tgtggatggt tacactaaaa atgaagttgc ttataccaaa gaagaagctg
     3661 ctgcttattt caaagaacaa tccgagacta ccaacctgcc atttattttc ttgagcgcag
     3721 gagtaactgc tgaattgttc caagaaacct tgaaatttgc aaaagaggct ggctctacat
     3781 tcaatggggt actctgtggg cgcgcaactt gggcgaatgg tgtagaacca ttcgttacta
     3841 aaggtgaata tgctgccaaa gaatggatgc agacacaagg acgcaagaac atcgaagagc
     3901 tgaataatgt tttgaaagaa acagcaacag ctattaaatt gtaagaaagc tttaactata
     3961 accttaagtt tgtaacctaa tgatttttaa taatcaattt gagcttttaa ataaagaatt
     4021 ggaagcgtcc cgacgcttcc tttttcgttt gtttttaagg catgataggt tctatgttgt
     4081 cgttcaataa gcgcaagaag gtcagcattt ttttcttaga gtatgcaaaa ttgcttttct
     4141 acccaatgat ttaaaacgga gaggcaccct tggatatggt agatggtgaa gtaacgcatt
     4201 tcttctgtga tcacggtttt tttgtcttct tgtagccaga tttttaaata attttcttcg
     4261 atcagattat ttaatctctt ttgaaattgg atatctccgt ggttgatgac gaataacttg
     4321 tatacgaacg tattcgtata gacatagtcg atcaattgag cgtatagatc ctcgtagcta
     4381 tgtgtcgtat cgtaggaaat gatttcatcc aagtcttgga aaaagctatt ttccagtttt
     4441 tcataaagat catagatatc cgtataatgt gtataaaatg tcgaacggtg aagatcagca
     4501 gtttcggtca gttcacggat ggtgatgttt tgaatttttt tatctactaa taattttgat
     4561 aatgcttctg ctaacgcttt ttctgttttt ttgacgcggc gatcctcttt tttcattttg
     4621 cagcctccta atcaacataa tcaaggaatt gtgtcggtta tccgacaatt tcaccgctat
     4681 ctgaacatag ataaccgaca ccttgtctat tataatataa gtatagcata tcaatattta
     4741 aagaatagga gaataagtga tggaattagc ctttaagcaa ctggaaaaga aagactacaa
     4801 aaaagccatt caattcgcga ttaaaggtat gcactttgat tggtatacca agaacaaact
     4861 ccttttgaat ctttatggaa gatacttttg gtatctagaa ttgaatcgcg cgacacaaat
     4921 cattgcttta tatgcggatg atgagttggc aggtgtttta ttagcagaca tcaaaggaaa
     4981 atcaaaaaag catcattcat ttagtcaaaa gttatatgtt aaactatttg atttcttaca
     5041 aaatgcattt gtgaaagaca gcaaaggcgg gtatgacgac accaatgacg aattgctttc
     5101 agactattta aaaaaacatt ccccagatgg agaaattgtc tttttagctg ccaaccctga
     5161 tttaaagatc aaggggatcg gtagtaaatt attaaaagaa tttgaacgcc gagagcaagg
     5221 aaaagaagta tttctgttca cggatagcgg ctgtacgtat caattttatg aacgacgcgg
     5281 tttcgaacgg aaaaaagaaa aagataacgt catgaagatc cttaataaag aagtagatct
     5341 ccgctgtatg ttatatagta agatcattca gtaatgtttt ttactatcag aaagcagaag
     5401 taccacgaag gtcaatggaa aaagcgagta attgatccat ttagatgaag ataggtgtag
     5461 tctcaaagat attagctggc aaagatatcc ccagttaaaa atagatttct gtatccaaca
     5521 ataaaaatat ttttgtattt
//
LOCUS       DAYYNG010000163         5483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000163 DAYYNG010000000
VERSION     DAYYNG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.165"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1153
                     /gene="uxuA"
                     /locus_tag="ACHBFA_002544"
     CDS             68..1153
                     /gene="uxuA"
                     /locus_tag="ACHBFA_002544"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9395962.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            1146..1859
                     /locus_tag="ACHBFA_002545"
     CDS             1146..1859
                     /locus_tag="ACHBFA_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9395963.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(2066..2215)
                     /locus_tag="ACHBFA_002546"
     CDS             complement(2066..2215)
                     /locus_tag="ACHBFA_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395964.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            2611..2811
                     /locus_tag="ACHBFA_002547"
     CDS             2611..2811
                     /locus_tag="ACHBFA_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9395965.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            3507..3941
                     /locus_tag="ACHBFA_002548"
     CDS             3507..3941
                     /locus_tag="ACHBFA_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395966.1"
                     /translation="MIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGND
                     HSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAV
                     LLNHVRKCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(4002..4367)
                     /locus_tag="ACHBFA_002549"
     CDS             complement(4002..4367)
                     /locus_tag="ACHBFA_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM9395967.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(4443..4688)
                     /locus_tag="ACHBFA_002550"
     CDS             complement(4443..4688)
                     /locus_tag="ACHBFA_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9395968.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(4758..5012)
                     /locus_tag="ACHBFA_002551"
     CDS             complement(4758..5012)
                     /locus_tag="ACHBFA_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395969.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(5176..5481)
                     /locus_tag="ACHBFA_002552"
     CDS             complement(5176..5481)
                     /locus_tag="ACHBFA_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395970.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT         1965 a          845 c         1082 g         1591 t
ORIGIN      
        1 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
       61 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
      121 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
      181 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
      241 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
      301 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
      361 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
      421 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
      481 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
      541 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
      601 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
      661 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
      721 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
      781 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
      841 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
      901 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
      961 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
     1021 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
     1081 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
     1141 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
     1201 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
     1261 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
     1321 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
     1381 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
     1441 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
     1501 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
     1561 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
     1621 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
     1681 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
     1741 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
     1801 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
     1861 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
     1921 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
     1981 aaacacaaaa cgcatctact gttatctacc agtagatgcg tttaaagaga agaacgagaa
     2041 tcgtccatat gagggtttcc actccttatg cagatttttt taattcttgc actgcttgtt
     2101 cgatcgtaac acctgtcgct acatgattgg tattttcttt gtcaaaaacg acaaatagat
     2161 tcaaatctgt atccaaggtt actcgatatt gtatatcttt gttatagaat gtcatagcca
     2221 ttctcctttc tttaaatact tgctgaaagg caacgaacgc gttgataacg aaattccatt
     2281 tataagtata ccaccaattg aaaggttttc atagttaaga aattttaaag tcaaaagttg
     2341 cagataaaag caaataaaca agaaaacaaa aagtgaatca atcgcggtta ttcaactttt
     2401 tcttcgattc gaagacaata gaagacaatg aattttatat ttcattgcat tatccttgtg
     2461 ttattagaaa aagcatgata tattaaatga gtaatctatt ttgaaacgta atttgagcga
     2521 tatatctaca tagataaaac tccttttgcc tatcaaatat ttattgcaac gaaaaaacgt
     2581 acattgatac gtatcaggag gaatttattt atgaataacg gtacagtaaa atggttcaac
     2641 gcagacaaag gttttggctt tatcactggt gaagatggaa atgatgtatt tgcacatttt
     2701 tcagctatcc aaggtgacgg cttcaaaact ttagaagaag gccaagcagt gtcttacgat
     2761 gttgaagatg gtcaacgtgg ccctcaagca acaaacattg ttaaagctta ataaaaattg
     2821 acttttaacc gtcttttcga aagaaaagac ggtttttttg tacgaaaaaa ttcattggtc
     2881 gttgaatggg ctgaatagta gtgtataaaa caattttgtg gagcgtgaag cttttatggt
     2941 agaggaaaat acgaataaag tgatgttgat aaaaagattc ctatcttgaa tatgaatcga
     3001 gatgtcttga aaatgaatga gtttgaccaa tcattatcct atcgaaattt acagtttcgt
     3061 tcgattggtc tgtcaattaa tgaaaaagta aaaaagaata tgttctagga agagggcggc
     3121 acatctaaat ttttaagtaa cggacagttc aaatatcaag cattgaaaga ggctatccta
     3181 ctgtttgaag gaatacgaat atataaaaac gacaaagagg aaaaagtagt tcattaaatc
     3241 ccttcaagtg atgaaagaat gagatctaat aaaacctgat ctttcatctg tttttttaat
     3301 aacgtaataa tttttttatt ttttgtgctt tcgccgttat tgtagagtga tttagcattc
     3361 ttatttttaa aaataactgt gttatatatt aaaaaaggca taatagttat aagctttttt
     3421 aaagaatatt tagaaattat taattttcgt ctgaaaacaa aaaaagaaag cgttattatt
     3481 tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta tcaatattaa
     3541 cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc acaaaatcaa
     3601 ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac agaggcttaa
     3661 atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa
     3721 gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc aatctgtgct
     3781 gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg aaatgttcat
     3841 ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt tttgcacgtg
     3901 aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg gttaaagctt
     3961 tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag ataaattcct
     4021 ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc cggataaatc
     4081 ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt gccacgatac
     4141 ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc atccatgaca
     4201 agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc cacagtagca
     4261 tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg tcctaaaagc
     4321 tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga aatgtctcct
     4381 ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa
     4441 aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg
     4501 taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc ttcttcgtac
     4561 ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg agataaagat
     4621 cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg
     4681 atactcattt aatcaccttt cattatagta gaatagttat aatgactata gaaagctaca
     4741 catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag aatgacgatc
     4801 aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg ttcaaagtag
     4861 ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat agctaaataa
     4921 aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc gaccgcaggc
     4981 acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg attcatataa
     5041 taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat aaaagaagaa
     5101 tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg aactgttccg
     5161 ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt aattcaatac
     5221 atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc catagcgaat
     5281 ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc ctttatagct
     5341 gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc caataaacag
     5401 aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa ctaaatcgaa
     5461 taaaatcgca ataaatggca aaa
//
LOCUS       DAYYNG010000164         5479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000164 DAYYNG010000000
VERSION     DAYYNG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.166"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..410)
                     /gene="ssrA"
                     /locus_tag="ACHBFA_002553"
     tmRNA           complement(44..410)
                     /gene="ssrA"
                     /locus_tag="ACHBFA_002553"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(581..1354)
                     /locus_tag="ACHBFA_002554"
     CDS             complement(581..1354)
                     /locus_tag="ACHBFA_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9395971.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1498..1848
                     /locus_tag="ACHBFA_002555"
     CDS             1498..1848
                     /locus_tag="ACHBFA_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9395972.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1829..2545
                     /locus_tag="ACHBFA_002556"
     CDS             1829..2545
                     /locus_tag="ACHBFA_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM9395973.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2545..3993
                     /locus_tag="ACHBFA_002557"
     CDS             2545..3993
                     /locus_tag="ACHBFA_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9395974.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4063..4572)
                     /locus_tag="ACHBFA_002558"
     CDS             complement(4063..4572)
                     /locus_tag="ACHBFA_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395975.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4562..5287)
                     /locus_tag="ACHBFA_002559"
     CDS             complement(4562..5287)
                     /locus_tag="ACHBFA_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395976.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1865 a          955 c         1095 g         1564 t
ORIGIN      
        1 aaaataagga tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga
       61 gtcgaacccg cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt
      121 aaggattcac tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct
      181 ctttttcaac ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc
      241 gagcacatgg gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa
      301 gagttgtttt cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct
      361 acgaaacgca attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac
      421 aagggttacg tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata
      481 aaaaagcgaa tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa
      541 gcgattttct taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact
      601 tcaatgcgtg gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt
      661 ttaatccgtc ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa
      721 aatcattcgg accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt
      781 gcagacgttt gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag
      841 gaccattccg ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt
      901 gagataagat cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg
      961 gaacatctga atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc
     1021 agattttctg atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca
     1081 ttttttgaca ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa
     1141 tgaaagaacc attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga
     1201 tttctgcttc tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt
     1261 tcatagcttt tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa
     1321 gatcaaagaa agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat
     1381 aagtttatct taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat
     1441 attggttcag tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg
     1501 atttttttca gtattggtat cttattctta ataattggca gtctttttcg gattctgccc
     1561 tcaaaaggaa atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct
     1621 cattggcggc tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg
     1681 gcattgatcg gctattattt aaagacatct gggcatacga attattttct gattgaaatg
     1741 cttctgctag tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa
     1801 tatgacctgg aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc
     1861 tgtttctgaa atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta
     1921 tgatggaaaa gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac
     1981 actagacctg aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt
     2041 ttcaagtact gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat
     2101 cggcttagag atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat
     2161 tgctcgaatg aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt
     2221 agtttcagat gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg
     2281 agaagcgtat ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt
     2341 gtttacttta gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt
     2401 ctgggattat cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg
     2461 acaaaaaatc gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa
     2521 attcgatgat tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc
     2581 ggaatggtcg cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa
     2641 acgattgaag aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca
     2701 acgcaaacct atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca
     2761 ttgctgctga cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa
     2821 aacgaacgtg tcatttatcc gactaataca gctttactga attttacctt aacagctgat
     2881 caatggaaaa atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta
     2941 ggacaagcac aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat
     3001 ggagcacttg tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca
     3061 ctgaacctgt tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct
     3121 tttgcagcat tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg
     3181 aatggtaatt ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca
     3241 gatgatttca ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa
     3301 gaaaatcgtc gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg
     3361 acaatcaacg gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa
     3421 aacgcgatta aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac
     3481 ttggattatg aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga
     3541 acagaaacat tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat
     3601 acattgacat tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc
     3661 caaatcatgg tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg
     3721 attactttag aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga
     3781 atgtctgaac aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga
     3841 aaaaatacaa aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc
     3901 ttgcataaag gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt
     3961 cgtttgcctg acgtagaaat caaaaaagag taaaaaacaa gcggtataaa agatcatgaa
     4021 tcgatcttct ataccgcttg tttttactat aaaatgattg atttagcccg cgggctctaa
     4081 acgatcaata taaggacttt gagcaatagt agcatcatga gtaaagagta agataccttg
     4141 attttcttca tgagctaaat aagaaagcaa gtggagtagc tcttggatct ccaatacact
     4201 aagttcatca aatgtatctt caataacaat attgtttcgc ttggaaagca aggcatgaag
     4261 taattgcagc tttacttttt caaaagacgt caactcagct gccgcacaat taagcaagga
     4321 aggagagata tcaagttttt cttgataggc caaacgatct tgtttgattt ctttttccgg
     4381 tacatccaac aggacatggt ctttaagatt cagataagga aacatcggcc aatttttttg
     4441 gatgatcgca aaattatcca gttttgcaac aagaacctct tttttcaagt gctccaatat
     4501 tttatgctgt ttttcgagag gacacaaaat aacggtcaag tgttcagcat gaatcaaata
     4561 ttcaggtttc attacgtcca ggcctcctac tttgcaaatg atggataaaa aaagtgaaag
     4621 ggaatagact aagaatgaga cagcatccac tgagcccaat aagagtttgg gcaaatccct
     4681 taagtatatc tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat
     4741 ccatcggaat tgtaagtccg cttaccgaag tcaagatatc acggatatct ggcagttcag
     4801 tagtctttaa gacaagaaaa ttgatccttt gaagaagcgc ttcataaact gactggaagc
     4861 ttacgatcca caataaaacc aagatacaac aaagaaagag aggaaagaac agctgccaga
     4921 aaataaaaag gaataatttg gttttagaaa gaccagtcag gtgccaattg atcaattcct
     4981 gcttgttttg cctgactgaa tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa
     5041 ataaactgaa tatgcctttt atataaaatc ggctagctaa ttctaaagcc ggaagaagct
     5101 ctgtagatgc agaagcgcca ataaatgaaa gccgatcttg gacttgattg gctaaagccg
     5161 attgcaaatg gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag cttgaaaata
     5221 aacaatagag tattgtctgc tttttgtaat aaattaatcg agcagtagca tagcggatta
     5281 ttttcatact tttcacctct ataaaaagta tagaaaatga atatgaataa agtatgaaat
     5341 tcctttagtg aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg
     5401 aagaagctgc cctattctat taccgtagat gcaagctttt gaacactgtt tattcgaaaa
     5461 gaggagcagc gacaataat
//
LOCUS       DAYYNG010000165         5426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000165 DAYYNG010000000
VERSION     DAYYNG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.167"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..426)
                     /gene="prgO"
                     /locus_tag="ACHBFA_002560"
     CDS             complement(151..426)
                     /gene="prgO"
                     /locus_tag="ACHBFA_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM9395977.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(398..1351)
                     /locus_tag="ACHBFA_002561"
     CDS             complement(398..1351)
                     /locus_tag="ACHBFA_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9395978.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1963..3456
                     /locus_tag="ACHBFA_002562"
     CDS             1963..3456
                     /locus_tag="ACHBFA_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM9395979.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3591..3887
                     /locus_tag="ACHBFA_002563"
     CDS             3591..3887
                     /locus_tag="ACHBFA_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9395980.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3988..4839
                     /locus_tag="ACHBFA_002564"
     CDS             3988..4839
                     /locus_tag="ACHBFA_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395981.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4860..5072
                     /locus_tag="ACHBFA_002565"
     CDS             4860..5072
                     /locus_tag="ACHBFA_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395982.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1867 a          836 c         1013 g         1710 t
ORIGIN      
        1 tttagtatag taaaaactag aaacaataaa atttgtgaat gttgtatacc cccaaacaat
       61 acacatattt tgaaggatta ggattaataa agctaaaaat atagtaagta tactaaatat
      121 atttactata tctagtatac ttactatatt ttaatctttt gatcgtctat tatatataat
      181 ccgttcctcg tcgtttaaag tatcaacata ggcgtccatt gcaagtttga ctatttcata
      241 cataggaata tcttttgcat aactaagatc ccgaatggtt tttagtacgg gaggatctac
      301 ttgaactggt tttctttctt tcgcagtgta ctttcgttcc cctaactcga cattaatatc
      361 atctttaatt gaaatatctt cagcaccaat tttcttctta tctttgttcg ttggtaataa
      421 tgccatttag tttaatctcc tttccaagag gtaggatatg attggccatt gagtcagcat
      481 atttaggtgt ataatcaaat ccttcaatat caccatgttg ttcatagtat tcaattcttt
      541 cttctaattc tgtaaagatg tctgcaaaaa tagcccacca tcttcgatct acataatcat
      601 ttaattggat accattctca ccatacattt ctaaccgatc cgagccttta acaatgcttt
      661 taaaaatgtt tgattcacca tacttatcaa ttgttttttg atagtttgcg tgttgagagg
      721 aacgtcgcac tgagaaaaga acaggcaaaa ttccaatgat ttgaagatga gactcttcaa
      781 aattcgtgac aatcggaact aaaactttat taataaaatc ttctgttcct tccattgcgt
      841 accgttttaa ttcttgcatt gcaataatat aatctgcagc agttaagaat gcacgaacca
      901 cattatctgt tgatggtggg cagtcgataa agatataatc gaaatttttt ctcaattctt
      961 ctaaaggttt aatgaaggcc aatgatctct tacgacgatc aggatatttt tcggacaagt
     1021 attcattaag ttcttcagat ccaggagagc cagccataat atataaatgt ggacttactt
     1081 gagtaatacc tgaaacccag tccccttttt tgattgctgc agacatggat cgatcaaagc
     1141 tagtaatttg atacgttgat ccaatgttgg aagtagtatt tctttgttgg tcataatcaa
     1201 ttagcaacac gtctttttca aaaagttgac tagaaacaat cgctaataaa tcattgtctg
     1261 tagtcttacc aatgccgcct tttctacctg taagcattac aacttttcct agtttatctg
     1321 atgttttaaa tgcagtccgt aagttttcca tagttccctc caatttccta tactaaatat
     1381 atttagtata cttcatatac ttaatatatt cgatatatct aatatactcc aaattgataa
     1441 gaattcagac tttataagga atatagtgga tatactaaat atatttgcta tattcagtat
     1501 atcagatata ttcaaaatgt caaaatatat ttgtgcgttt tgcgttgaca atttagagtg
     1561 aaaagaggta aacttaattc tatagaaacc acagataatt cactatttac aaagtagcaa
     1621 aagcagtggt actctttaaa cagaaaaatt acataaaaaa aagagacgga aataattgat
     1681 tttagcagaa tcaattattt ctgaatcaca tggttaggac atgtgttcac gctcgtctca
     1741 agaatatgta agtagctacc aactacttat attaaaagtt gatcggatac cttttccgat
     1801 tgcctttatt ctaccaaaaa aaggcgagca actcaacaga agaaagttta tttagtgtac
     1861 ttaaattaaa tattcttaaa cactgaagag agaacacata ctatggctga gattaaagct
     1921 aaactgtgtt ctctttttgt ttgagaaagg acgttacaat cgatgttgag cgaaatttat
     1981 tcagctattc ttaaaaacgg cttacgtaca tacaaatatc gaaacagcac catgaaacct
     2041 gcaggtcata ttgattccag taggagtggt gctatctttg cgtaccgtag caagcaatta
     2101 atgaatgttg ggcgtggtat ggtcattact tcggaagaag ctattttaga gaacgaaaag
     2161 aaactaacgc attggacacc taatacctat cgctttggta cttatgcgga tgattatcga
     2221 acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa tcaacacatt tgtagttgat
     2281 atcgattcaa aagagaacca tcaaggcgaa attattttag cctgtttaga tcaagtgggc
     2341 tacatgccaa cgctaatttt agaatcagat catggttatc aagtttattt tgtactaaag
     2401 acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga tcgatgtagc aaaaaaaatt
     2461 tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg gagtggatct aggatgtaac
     2521 cacttcggaa ttgctcgatt cccacaagaa aataatattg tttttaaaga aatgaactat
     2581 cagtattctt ttgctgactg gattcaatgg tcgatgaagc ttgaatcaga tcaaaaaaca
     2641 aaagaagatc gagaacgaaa actaattgtc tttcctgaga aaaaagaaat tcgtcaagta
     2701 gatgaaccgt ggttcgatat gttacttcat aaagcaaaca tcgtcggcgg agaaggacgt
     2761 cttggtcgaa ataatgtgat tttcacgctt tctttagctt attactcttc aggttatgca
     2821 ttggatactt gcgaatacaa tatgttcaca ttcaacgaac ggttacagga accgctatct
     2881 gaagcagaat tagtaaagat tgtacgtagt gcttattctg gtaattatca agcagcaagc
     2941 agagaattca ttattgaatt gtgccagcaa tgggtttcac caaccttaaa aaataatgat
     3001 ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc cacgagatca aagaaaatat
     3061 agtcataaaa atgaatggaa agaagatcta ttggcttatc taagcgaaaa aagtcttgct
     3121 agtgagcctt atttgaacct gtcaaaaaaa gaattggctt cacaactaac gatgcccaag
     3181 cgaacacttg ataagatctt aaatgagtta cgagaggaga ataaagtctt ctacaaggta
     3241 acacgaggaa gaaatggcgg aatgatcatt gcttcagtca aaacgttgtt cgcgagggtt
     3301 attcaactaa gtaaagaaaa gagactagat tattcaacaa ggatcacaga ggtatttgga
     3361 ctcaacgaga gcttagtaga agagacattt agacaattat caaccccctt gaaccctctt
     3421 aaagaagtga atctttttga agtagataca ggttgaaaaa taaaaggtgc aaattgccct
     3481 atacaatata tctatgatac ttctgtaggt gttgctccct ttgcctagct acgtgtaggt
     3541 ggtatactta taactgcgta tgcagtctta tgtatcatgg aggaagttgt atgtcgttaa
     3601 aaaattatgt ctattcacat cctgttaatg tgtttatcat tggtcgtttg ggattatctg
     3661 tggaagtgtt ctgtgagctt tatggtttta agcaggggac tttgtcttct tgggttacca
     3721 gggaaaaaac agttgcttct ttgccgattg agtttcttca tgggttgtcc ttagcttctg
     3781 gggagactat ggatcaggta tatgattgtt tgtgtgtgtt agagcaggag tatattgagt
     3841 ataaaatagc taacgagtta agaaaaagga agaagtatat ccagtagtag tttttattta
     3901 cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa
     3961 aggaaagaat ttagggaaga aatcgaaatg aaatcggacg actggattga acacacctgg
     4021 cttcctctga aaagaaatag caaacgaaag aaattttggg tattgcaatt atggccatta
     4081 ggaaaacttt gtagttacgg ggcacgactt attcttgatt taaccgttta ctcgattgga
     4141 gtatttggaa cggcttatta cttgataaat gatcccaata ggattgcagc atcagacatt
     4201 tttacagtgc taatgacgtt gatttttgtc atgctattgt ctatttttac tcaattagtc
     4261 atgcaattta ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct attcgattta
     4321 attgttgtaa atgcaattct agcgacttta ataaaacaaa ttgaacaatc tggaagaaca
     4381 acaaaaggag tgaaggattt tttattgacg tttattcact ttgtaaatgg acacatgtat
     4441 ttgtttgaag tgggtattat tctctttttt gtcgggtatg tatccacgaa acttattaaa
     4501 cgttcaattg aaaaagaaat tgaaattgac ctgattccga cacttcttgg ttatgagtac
     4561 aatgttgatt ttggaagata tgacgaattg atcaatacgg atatcgttca cacgagtatc
     4621 catgaatcta ctgttaaggg catcagatct attttaaaaa atggccagga aatgaaagca
     4681 atagatcgaa gaaaagagat ctacaatttt tatgaagtcg aagcaagcct acgatatggc
     4741 atcggaaaat tgtggttaga tggaagtaat attcgtcaaa acaaaccata cattttttgg
     4801 aacagtagtg atttagaact tccaatgaaa gataaataat tatggagaat cgtttgaaaa
     4861 tgattcctct atacaggatt gtgcttatag ttggcatgga gttaaaaacc ttaaagaagg
     4921 agcagttatt caaagtgaaa gaacgtcata atcagttact atgtagtaaa acgggtacga
     4981 tgaacattga ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgttga
     5041 ttcgaaagca acaagagcaa gaaaaaaatt aatggctact tatagccaat tgattgatga
     5101 atgggaacaa ttcaaggaat ctaaaattcc agaacgtctc taaaagcctt tatttgtaaa
     5161 gagcataagg aaacgtggga acacttttta aaaaaagtgt tccaataggc tgtgcctatt
     5221 acgtttctca aaaataaatg tgggaacaaa atatttaaaa ataggatagt gaaaggatag
     5281 tgaaggatag taaaaggagg gtagtaggat agcaaaaagg ataggctcct tttcgctatc
     5341 ctttttaaat tggaggaatt aatatggaag taagaataag aaatgtggat gagaaaatcg
     5401 taagagaaat tgatcgaaga tgtgac
//
LOCUS       DAYYNG010000166         5387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000166 DAYYNG010000000
VERSION     DAYYNG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.168"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..352
                     /locus_tag="ACHBFA_002566"
     CDS             <1..352
                     /locus_tag="ACHBFA_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395983.1"
                     /translation="QMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKK
                     YPGLLHTSIQFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTR
                     NRNVLHQVAMEKAN"
     gene            411..851
                     /locus_tag="ACHBFA_002567"
     CDS             411..851
                     /locus_tag="ACHBFA_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9395984.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQITNKKQ
                     AKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            863..1531
                     /locus_tag="ACHBFA_002568"
     CDS             863..1531
                     /locus_tag="ACHBFA_002568"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9395985.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYASVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKKWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1595..2218
                     /locus_tag="ACHBFA_002569"
     CDS             1595..2218
                     /locus_tag="ACHBFA_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM9395986.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAKEFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2274..2660
                     /locus_tag="ACHBFA_002570"
     CDS             2274..2660
                     /locus_tag="ACHBFA_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9395987.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2873..4264
                     /locus_tag="ACHBFA_002571"
     CDS             2873..4264
                     /locus_tag="ACHBFA_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM9395988.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4476..4931
                     /gene="tnpA"
                     /locus_tag="ACHBFA_002572"
                     /pseudo
     CDS             4476..4931
                     /gene="tnpA"
                     /locus_tag="ACHBFA_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1807 a          891 c         1128 g         1561 t
ORIGIN      
        1 acaaatgcgt tatcaaatag atgacgaaaa ttctttttct atttttagac gtcagcaaaa
       61 gattctaaaa agctttttaa aagtgatcag taaaaaacgg aatctgatca aaacgacaca
      121 gctgttgaaa aaatatccag gtctgcttca cacgtctatt caatttcctc aattgattac
      181 gatgatccat cgttttatac gtatgcaaca gctaccatct aagaagctgt ttcttccatt
      241 gacggacaca tttcgagtag tcaacagaga agataaaaag aaagtgattg tgattgactt
      301 taccagaaat cggaatgtcc ttcaccaagt tgcaatggaa aaggccaatt aaggcttttt
      361 ctttttggat ttttttgtca taataaatat tagatgatgg aggagataat atgatgcaaa
      421 catggttaga aaggctaata cgatttcagc agaactatca caaacttatg cagggaagat
      481 atgcgaaatt tgatcagttg aatcgcggtc tattgatcgc tgcgcttgtc tttagtttgc
      541 taagtcgttg gttgccctat agaatcggac aattgttgtt catcgtcttt ttcggatgga
      601 tgatatttcg tttcctatca aaaaaaatct accccagact aaatgagaat caacgttatt
      661 tgaaatatct tgatagagtc aaagaaaaat atcatcagat aacaaacaaa aagcaagcta
      721 aaatggatcg aaaaagttat actttttttg agtgcccgaa ttgtcatcaa aagcagcgtg
      781 cgcctaaagg aaaaggacga atacgcgtga catgcaaaac atgtggtata aaattcgaaa
      841 cgaatgttta agggagaaaa gaatgaaatt agtattatta cgtcatggcg aaagcgaagc
      901 aaattttgaa aattactgga caggatggct agatgtcgcc ttaacagaaa aaggaaaaga
      961 acaagcagca gtagccggtc aaaaaatgct tgaagctggc cttcaattcg atgccgttta
     1021 tgcctctgtt ttgaaacgtg cgattcttac cggacaagtc gcactagaag aaatgggtca
     1081 gttatggctt cctataataa aaacatggcg tctaaatgaa cggcattatg gggatcttgt
     1141 gggtaaaaac aaagataaga tgaaagaaat atacggaaaa gatcaagtaa aaaaatggcg
     1201 cagaggatat acggaagttc cgccgttaac cgcagaaaat agatttgacc gcagatatga
     1261 tcacttggac ccgcatttaa tcccatacgg agaaagtctg gaaatgacag tgaatcggat
     1321 cattccctta tggcaagatc atctagcacc aaaactaaaa gatcggcaag atttactagt
     1381 tgtgggtcat ggtaatagca tccgagcact gacaaaatat ttagaagatg ttcctgaaga
     1441 tcaaatggat acgatcgata tcccaaatgc acaaccaatc cagtatacat tagcagatga
     1501 tttgacaatt ctggacaaaa aaatacttta aagaacacct ttatctaaaa gtgagagata
     1561 gaaaaaagaa gcaaaggaat ggaacaaaag gaacatgaaa aattatatat gggactttga
     1621 tggtacattg tttgatacgt atcccgcaat ggtagatggt gcatggcaag cactaaaaga
     1681 ttttggtatc agtatggaca aaaaagaaat ctacttcaaa atgaaaaagt attctactag
     1741 ttatttgatc aatgaatcaa acttgaatgc caaagagttt aatgaattat ttcaccgata
     1801 tgaaaaagaa agtacggaag tttcccgtcc atttccagaa acgaaacaag tactggaaat
     1861 gctaaaagac aacggcggtc gtcatttcat cctgacccac cgtttgacag aatcaacatg
     1921 gggcctgtta aaagaacatc gattggcaca tctgattgag gaagtagtag ggatcgatca
     1981 ggactttcct cgtaaaccgg atccagcatc tttaaattat ttgatcgata cttttcattt
     2041 agaaagaaca gacacgatga tgatcggcga tcgtcgttta gatatcgaag cagggaaaaa
     2101 tgcaggagtc gctacttgtc tttacgacat cgatcatttt ctgggtgaga tcccggcaga
     2161 ttatgtggta ggaaatctga atgaaatcct taccttgcag agcatcatta aaaagtagta
     2221 tcccctttca aaggagatat gttataattt tcataaagga gtgataaaat tatatggaaa
     2281 tcagaagaca aaaagaagtt gtagaagcat ttcattatga tatgagattg ccgaaccaag
     2341 aagtagaaac agatttgaaa gtgggcttct cgcctttaaa agaaacgatc gaaaactatc
     2401 cgaaagaaca tagcatcgta ggagcacgct tagagttccg tttagtgttt gatacatatg
     2461 ttttatctgg aagtgtgagt cagatcaatc atatcctcaa tcggaaaatt gaaggtcaaa
     2521 atgatgtcac acaagaagaa gtggacgagc tagtagcacc gttattcgat atcgtacgcc
     2581 gaatgtctta tgaggtgaca gagatcgcct tagatcagcc tggggtaaaa ttgaattttc
     2641 aatcagaaaa acaagaatag acagtaaaaa catacctgcg atagattaaa gctatcacag
     2701 gtatgttttt ttattactaa ttgtaagcaa aaagaatgtc atttcgataa aaatcgtcta
     2761 aaatataaaa gtcactgaaa aaaatttttt caggaggatg gttgaagaat cattctcggg
     2821 aacctttact tccttcgatg gacttgtcat taataatatg aggaggagaa aaatgacaac
     2881 actcatcaca gttttagtgg ttctagtttt tgcggcatta ttatatgtgt tctatcgtat
     2941 gcaaaagcgc catacaaaat tttcaacacg cgtctttaca gcattaggag ttggtatcgt
     3001 ctttggcggt ctgcttcaat tgatctttgg tgtagacgac aaagtcgtta ctcaatccat
     3061 ggaatggatc gggattgtag gtcaaggata tgtatcattc ttgcaaatgc tagttatgcc
     3121 tttagtgttt atttcgatca ttggtgcatt cacaaaaatg aaagtttctg aaaagctagg
     3181 gaaaattagt gcgactgtat tgacttcttt acttggaaca acagctattg cagcattgat
     3241 cggaattggt tcaacaatgc tgttcggact ccaaggagct aaatttacac aaggagctgc
     3301 agaaacagca cgaatctctg aactggcaac acgtcaggaa acagttgaaa acttatcgat
     3361 tccacagcag attttatcct ttatccctaa aaacgtattt gctgatttag caggtagtcg
     3421 tgcaacaagt acgattggag tcgttatttt cgcggcattt gtcggaatcg cttatctagg
     3481 cgtgaaaaga aaagcgccaa aagaagcagc attcttttca gatttagtcg acagtctgta
     3541 taaaatcatt atgcggatcg tgacgcttgt tttgcgtttg acaccttatg gagtatttgc
     3601 tttgatgaca aacgttttgg caacaagtga ttttgcagct atcgtcaatt taggtaaatt
     3661 tgtcgttgct tcttacgtag cattgcttgc tgttttgatt gtccactcac tgattttgat
     3721 tggtgcaaaa gtcaatccaa tcaactattt caaaaaagcc tttccggtat tgagctttgc
     3781 gtttacttca cgttcaagtg ctggagcttt gccattgaat atcgagactc agacaaaagc
     3841 tttaggagta gatgatgcaa cagctaattt ctcagcaagt tttggtttgt ctattggaca
     3901 aaacggctgt gctggcgttt atccagctat gttggcaacg atcgtcgcac cagcagctgg
     3961 agtgaatgtc tttagtttgg aatttatttt gatgcttgtg gcaattgtaa caatcagttc
     4021 ttttggtgta gctggagtcg gtgggggtgc aacatttgct tcactgatcg ttttaggggc
     4081 aatgaacttg ccagtagcaa tcgttgggtt agttatctct gtcgaaccat tgatcgatat
     4141 ggcacgtaca gcagtaaacg tcagcggtag catggtagct ggtatcgtga caagtgcacg
     4201 catcaaagat ttagatcgcg atgtcatcaa tgatgattct aaagtgatcg atgtgaatgc
     4261 atagtcatag ttaaaacata ttaggaaata tactaagatt atagggcaaa atcatcgata
     4321 gatgattttg ccctatattt ttttgtgtgt tttgaaaacc acctgctatg caggtggttc
     4381 cggaaagctt ctagcgaggt ataaaaaaag ccacctaaac tgataagatg aggtttcccg
     4441 accaccacat caaaaagttt aggaggtacc tttttatgaa aaaggctaca gaaagtttat
     4501 cacacactgt ttggaaatgt aagtatcata tagtattcgc tccggaattt cgtcaccaaa
     4561 tcatttatgg caattataaa gcaagtattg gagagattat acgcgaatta tgcgaaagaa
     4621 agggcgttga gattttagaa gcaaatgctt gtccagatca tatacacatg ttagtcagta
     4681 tcccaccaaa aataagtgtc tctcaatttt taggttacct aaaaggaaaa agtagtctaa
     4741 tgatttttga tagacatgca gaactcaaat atcgttatgg aaatagaaag ttttggtgta
     4801 gaggatatta tgtggacaca gtaggacgaa acaagaaaaa aattgcagaa tatattcgta
     4861 atcaattaag agaagaccac gtagcaaatc agcttacatt gtttgaagaa gttgatccgt
     4921 ttactggaga aaaaaccgga agaaacacta agggcttctt tgagaagcag cgagtaaatg
     4981 tggagcaaga gggaaccgat tcggtaagcc tttcaggctt aggccggtaa aagaggcttt
     5041 cagtcgcaga acaaaccacc cgttcatacg ggtggttttg attatttttg gattatagtg
     5101 aggagtgaaa gaaagttcga acaaaatcgt ccttcttatt tacgtatgtt gagactataa
     5161 gttacatgat tttcggccta atctaatagt tttgttcaac catttaaact taattgcgta
     5221 cttacctatc cttttaagct tatcagtgaa gaatcaaact ctctatacta gcttttattt
     5281 tatcagataa atagctcaga agatcatggt aggcgtataa ataattcatg tagagaattc
     5341 actttttatg ctatcctaaa actaataaaa agattatttg gtcttac
//
LOCUS       DAYYNG010000167         5287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000167 DAYYNG010000000
VERSION     DAYYNG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.169"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..305
                     /locus_tag="ACHBFA_002573"
     CDS             <1..305
                     /locus_tag="ACHBFA_002573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9395989.1"
                     /translation="EGPLVRTFRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLR
                     LRKQHYHCQSCGKYFTAHTYLVAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            462..719
                     /locus_tag="ACHBFA_002574"
     CDS             462..719
                     /locus_tag="ACHBFA_002574"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9395990.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            806..2044
                     /gene="istA"
                     /locus_tag="ACHBFA_002575"
     CDS             806..2044
                     /gene="istA"
                     /locus_tag="ACHBFA_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM9395991.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDEI
                     RAYTRESLKQYDLVEVADEE"
     gene            2034..2774
                     /gene="istB"
                     /locus_tag="ACHBFA_002576"
     CDS             2034..2774
                     /gene="istB"
                     /locus_tag="ACHBFA_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM9395992.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            3193..3348
                     /locus_tag="ACHBFA_002577"
                     /pseudo
     CDS             3193..3348
                     /locus_tag="ACHBFA_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3415..4842)
                     /locus_tag="ACHBFA_002578"
     CDS             complement(3415..4842)
                     /locus_tag="ACHBFA_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM9395993.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLRKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(4839..>5287)
                     /locus_tag="ACHBFA_002579"
     CDS             complement(4839..>5287)
                     /locus_tag="ACHBFA_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFM9395994.1"
                     /translation="QQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
BASE COUNT         1812 a          993 c          906 g         1576 t
ORIGIN      
        1 tcgaaggtcc attagttcgc acttttagac attgtctatt ttgtaaagaa tctgcaaagc
       61 agatcgagaa aaacggcaag aagatttcaa tgattatctt aaatcgcagt gggaataaac
      121 aaacctattt gcgtcttaga aaacaacatt accactgcca atcatgcggg aaatatttta
      181 ctgctcatac gtatcttgtc gctccttcat gttttatttc gaaacaagtt cattacacga
      241 tattagaaga gttgaccgag cgtcaagcat taaagacaat tgcgaaacgt tgtgattttc
      301 agtaacgaca gttcaacgga cattatctgc tctatcggca acgctgcgac agaagctaga
      361 ctatttacct cattgtttac tattcgacga gtttcgctct ctttcaattt tccatgggaa
      421 gttctccttt tcttgtatga attgagagac agggaagctc tttgacattt tgtccagccg
      481 gagaaaaaaa agatttgatt gcttatttca tgcgttttac aagaaaagct catatggggg
      541 ttcgatacat tgtcacagat atgaatgccc cttatttttc attagtcaaa gaacccttcc
      601 caaatgcaca aatcatcatt gattgctttc acattgggca acacttgaac aagtttttcg
      661 atcgtgttcg aaaacgtgtg atgaagcagt tgaatcaaaa atgtcaacgg cacctttaaa
      721 atgtaggaaa acggcaattt aaaattgttg gtttacctac atttttgtat gctcttctta
      781 tcccaaatga aatggaggct cagggatgag aaaagacata cgcgaaggag tgatgatcta
      841 cgtgatcaat gaaatcaaac ccaattatgc tgcactagca aaacaatacg actgcgacta
      901 ccgaacagtt aagcatgctt acgaagaagc ccaagttaaa gaatcaaaac ctccagaacg
      961 taagaagcgt ccaagtaagt tagatcccta cagagaaatc atccaagaca aaatcaaaga
     1021 ccaatgcagt gcttacagca ttttcaagtt tattgagcac aaaggatttg gaggaagcta
     1081 cagtttagta aagacctatt gtcgccagat taagcaggcg aaagtacaca aggcaaccgt
     1141 ccgagtcgaa catagccctg ggctttctgc tcaggttgac tggaaggaag atatgcgact
     1201 aatcagtaat cagggcgaaa tttttgagtt caatatcttt ttgtacgtct tgccatattc
     1261 caagaaaaaa tatgtcacac taactttcga ccgaaaacaa gacacactct ttgagtgctt
     1321 agatgatgcg ttttatcaaa ccggtggcgt tcccaaagaa atctggttcg ataatatgaa
     1381 gacagtcgtt gaccattctc gtagtcagtt ttccaaaccg catttcaaca aacgttttta
     1441 tgctttcagt aaggatgccg gcttcacacc aattgcctgt cggccatttc gtccgcaaac
     1501 aaagggctgt gtcgaagctt tagccagaac tgttgaccgc ctacgggtct ataatcatga
     1561 attttatgat gccgttgagc tgtgtcattt agtgaataac ctttgtgttg agttaaataa
     1621 cgaggtttct caatcaactg atcaaacacc agatgaacgt tggcagacga acgaaaaaga
     1681 gcacctgcat ccataccaaa aaaacctttt gaatccctat tttgaagact ttattcgccg
     1741 taaagtcacc aaggaagaaa tggtcatttt ccgtaaatgc aggtactctg tggcgcctcg
     1801 ctatatcggt aaagaagtcg aaatcgaact ctccgataaa gaagaccaag tacaaatcta
     1861 ttataacgga gaactgattc ggtcgcatcc attaacgaca aaacagctga actaccacga
     1921 ggaagactat ttccaaatct tgaaatccga tgtcatgagt cacaaagaag acgatgaaat
     1981 cagagcgtat actcgtgaaa gtctcaagca atatgatctg gtggaggttg ccgatgaaga
     2041 atagccatta ccaagaactg atgaccaact tagagactct tggtttgaat catatgcggg
     2101 aatatgttcc taactacatc gaagtggcca atcgagagga gctttcttta acggaagctc
     2161 ttctcgagct gacacgcaga gaagttgatc atcaaaataa gcaaaaaatg aatcgtgtgg
     2221 tggcacgcgc ccgcttttcc aagagaacca gcctggaaga gtttgatttt gccttccagc
     2281 caagtatcaa taagcgcgaa gtgctagact taaggcacat gggctttgtg gaaaaacatg
     2341 agaacttggt ttttatcggc agtcctggtg taggcaagac acatctgacg attgcattgg
     2401 gaatcgaagc ctgtcgtcaa ggctatcgga cactattcat cacctgtcat gagctgcttt
     2461 tgcgtctgcg aacagcctat gaaaaaggta ccctagagcg tgttctcaaa cgatacgctc
     2521 gctatgacgt cttgattatt gatgagattg gctatcttcc gattcaaaaa caggaagctg
     2581 atttattctt tcagcttctt gccatgcgct atgaacaccg ctctacgatc attacgacga
     2641 acattgtctt atctcgttgg ggggagttgt tccaaagttc tgagatcgca gctgccatcc
     2701 ttgaccgttt agtacatcac gtcaaggtgt tcaagatcac aggcaagtct tatcgtatga
     2761 gagacaagat ctaaagtgtc gattgtcgac aattactatt gccgaaaacc tacattttta
     2821 tcctgcccaa aacctacaat tttaagctgc ccttgacatc aaaaagacac ttcacaagct
     2881 aagtgctatc gacaattgaa acctttgtat aaattacttc tataatcaga tgacgagttg
     2941 gattatacaa cattcaagaa atggcagaat tttcaatggc gctatttaac agaaagtgaa
     3001 gtagttgatt gtctaccctc aatctcttat gaacttaaaa tggcttagca gtattatcaa
     3061 aagctgctta acaattatca caagaaacag ctagctactt cgagaaatga ccaaagaagt
     3121 tccctcgaaa cttcactatc ttaaaaagcc tttttcatat ctgaaaaagg cttttaacta
     3181 gcattttcga aaaagtattt taatacaaaa ttagagaatc ttcgcacaca tatcaaaaca
     3241 ttgaaacgat ttgcttatgg attttgcagt tttaccaata tgcgcttgtg gatttttcta
     3301 atcaacgggt taataagcta tgcattaaaa gctagtaaat cttactaact ttgttgatca
     3361 taaatgacct tatcagcccg atttgacaaa gaactacttt tttacaacat tattttattt
     3421 tcgaataatc tttcttactt catttcgtat atctggaaaa agaaaataaa caaacggaag
     3481 ataaataatg aaaatcatta ttgatttaat tgccatatct aaaaatgaag aagagaataa
     3541 ttgtggaaga aagtaatagg ccgaagtagt caaagtaaga ttgactataa aaaataagac
     3601 aacgttcttc ccaaagaaat aggatatttt tatttctttt cgtacaaaaa atactctaaa
     3661 gaaaaagact aataattcta ctaccacgag tacgattgta gccgctacag cgccataagt
     3721 aggcacaaaa gctacatttc caataatact tacaatcgca ccaatgatca ttggataagc
     3781 atactcctta tctttttcta tagcaattgc aaattggtta gcaaaaactc caccaattgg
     3841 actaacaact ataattagac aagaaataag catgtttatt gtcatcggtt tgaaattctc
     3901 cccaaaaaac catggaacaa attcttttgt attgataaac accacactaa aaagaattaa
     3961 tgcaataata atcgtgtaat caaaagattt ttttgtgtaa actaaaatat tttctgattt
     4021 tccctctgcc tgtgctttag atattttagg caacataaca atagtaattg atgttaagac
     4081 tgccaatata atacgagcaa ttttttgaga ctgatcataa taagataatg ctgttggatc
     4141 cacaaaatac ccaactatcg ttttgtctaa agttgtgtac acttgcataa acaactgagg
     4201 agtcaaaata ataaaagctg gtttccgcaa ctgtttaaaa acattagagt taaagagtgg
     4261 tttacctact tttccgtgga tactcaacca gaaaactaaa cttcctaaaa agttagtaat
     4321 tgaagtaatg aacacataaa tccacaaatc atctttagtt gaaacaaaaa ggattattaa
     4381 caataccgat gataatttga taactgtatt tctaataacc actttcgaaa attcttcaat
     4441 tccttgaaaa aaccaagaaa tatctaatgt tagccctgca ataaatatca attgaattaa
     4501 gtataaattt ttatacttta catcaaaaaa ataaatatag gctatataga gaaaggaaac
     4561 actcaagcct attaaaaatt gaagaaagta aatattccag aattgtaaag atagtgcttt
     4621 tttatctttc cttgatttag cgatatttcg aatacgaaaa ggattcattc ccacagcaat
     4681 gacagtcatt aaaaattgtg atattgagaa aacataccca taaatcccta aaccagttga
     4741 acctattcta cgactaagta taggtatagt aagtaagggc aaaacaatca aaaagacttg
     4801 atatatcgca ttataaaata tatcttttac tgtttttttc actttatatc cctctctaac
     4861 aactcaagaa ccttttctgt acttgcccct gtttcataat ttccaatata atcaagtaac
     4921 tcttgagaac ttttcaaaag cttttcttgt tggtttttaa aaacatcaac aaggccataa
     4981 ctatcttgta cgcttataat tggtaactca cttaactgta tataaaaacc tctttctaag
     5041 ctatattctt tttcatcata gttaaaaaga ataattggtt tttttgttag cgcaaaatcg
     5101 aaaaaaatag atgaataatc tgtgattaaa acatctatct cttcaaaaag catttcaaga
     5161 ggaatcccat cattaattaa agtaatttga tgttttttca tatattctat acttttagaa
     5221 tcagttgcta atttactatg aacattagga tgaaatttag tcattaaatt taattcttgt
     5281 tgttgct
//
LOCUS       DAYYNG010000168         5265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000168 DAYYNG010000000
VERSION     DAYYNG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.170"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..799
                     /locus_tag="ACHBFA_002580"
     CDS             47..799
                     /locus_tag="ACHBFA_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9395995.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            796..1710
                     /gene="pfkB"
                     /locus_tag="ACHBFA_002581"
     CDS             796..1710
                     /gene="pfkB"
                     /locus_tag="ACHBFA_002581"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9395996.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1728..3659
                     /locus_tag="ACHBFA_002582"
     CDS             1728..3659
                     /locus_tag="ACHBFA_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9395997.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3770..>5265)
                     /locus_tag="ACHBFA_002583"
     CDS             complement(3770..>5265)
                     /locus_tag="ACHBFA_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM9395998.1"
                     /translation="FVGLLTGIVVSLFRLSIEKLSDLVRSIYEMSREQPIYLIGLAIC
                     CILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSMNWFSVLWKKFIGGVLSVGAGLF
                     LGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSGASSGLAAAFNAPIAGLLFVLEE
                     IHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYLGDLPSLPLKYYGSLVILGVLLG
                     ILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLILPIGYLWPSTLGGGNQIVLAFGH
                     ASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPILTLGALIGSFYGNIMVDFGMDPI
                     YIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLNHLMPLGLAALVAYIVNDLLGGN
                     PIYESLLERLLGNTQTASLKGARTTFDFPVTAESSLDGLMVRDFNWPKNMLLISIRRG
                     NQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEAKSSITNLKQSF"
BASE COUNT         1722 a          999 c         1144 g         1400 t
ORIGIN      
        1 agtagaaaaa aaatatgcta ttatttgtat tgaggtgatc gtgaaaatgc ttacagagga
       61 acgtagacaa aaaatcctag acttgcttga acagcagaaa atcgtgaaat cacaagatct
      121 tgtaaacctt tttaatgctt ctgaatcaac gataagacgt gatttacaag agctggagaa
      181 tagtgggttt ctagaaagag ttcatggcgg agctaagaaa gagcaattat tgaattttga
      241 gcagaatatg agtgaaaaat cactcaaaaa cgttcacgaa aaacagcaaa tcgccaaatc
      301 ggcagctgaa accattaatg acgaagatgt catttattta gacgctggct ctactacgtt
      361 agaaatgatt ccttttctgc aaggaaaaca gatcacagta gtgacaaatt ccgttcaaca
      421 tgcggcaaat ttagttgatt tggagcttgc taccattatt ttaggtggaa cgataaagct
      481 ggcaactaat gctgttttag gatctaatgc gatagaacaa ctaaaaaatt ttcattttaa
      541 taaagcattt atgggaatga acggtgccca tcttgaacaa ggattcacga ctccggaccc
      601 agaagaggca gctgttaaac gtttagccat gaaaaattcc gaagagacat ttgtattgat
      661 tgatcatacc aaattcaacc agttgtcctt cacctctgtt gcacctttgg aagcagccac
      721 gatcataacc gagcgttgtc cattagaatt cgttaatgaa ttccgtgaaa agacaacaat
      781 taaggaggca atacaatgat ttacacagta acacttaatc catcaattga ttatatcgta
      841 cgcgtagatg ggttaaaact aggcgatttg aaccgaatga aggaagactt caagttacct
      901 ggaggaaaag ggatcaatgt ttcccgtatt ttgaaacgga tcgattctca atctacagca
      961 ctagggtttt taggagggtt cactggcgaa tttatttcga agtggcttga aaaagaacaa
     1021 atccagtctt tgttcacaac cgtaaaagaa gatacaagaa tcaatatcaa actaaaatca
     1081 gatactgaaa ctgaaattaa cggtttgggc ccatctattt cagatgaaga aatcgatgag
     1141 ttgaagcaag cattaggaac agtaggacaa ggagatatcg tcgtattgtc aggaagcgcg
     1201 ccggctagcc tgcgaaaagg attttatcaa gaactgattg atatcattcg agaaaaaggt
     1261 gcagatttcg tgattgatac aacaggtgat gacctaaaaa atgcacttgt taagaaacca
     1321 ttgttgatca aaccaaacaa tcacgaatta gctgagcttt acaatacaac cttccaatct
     1381 gtagaagaaa tcattccgta tggtaaaaaa ttgctagctg aaggggcaca gaatgtgctg
     1441 atctcaatgg ctggagacgg tgctttgctc ttcacggaac aaggaactta taaaacgaat
     1501 gtattagttc gcccattgaa aaattcagta ggtgcagggg attcaatgat tgccggcttt
     1561 attggggctt acagccgtac gaaagatcca atcgaagcct ttaaatgggg agttgcttgc
     1621 ggaagcgcaa cagctttttc tgatgacttg gcagctcgcg aattgattga tgagttgctg
     1681 ccagaagttg aaatcacaaa aattgattag tttcaaggag gataatcatg aatatcaagg
     1741 atttaatggt caaagaagcg atgatcatgg atttgcaagc gactgataaa aaaggcgcaa
     1801 tcgatgaaat ggtccaaaaa atgtttgatg ccggccggat ttccgatatc gaaacatata
     1861 aagaaggaat tctagcccgc gaagcacaaa cttctacagg tcttggtgat ggaatcgcca
     1921 tgcctcacgc gaaaaacagt gcagttaaag aagcaacagt tttattcgca aaaagccaaa
     1981 aaggagtaga ttatgaagcc ttagacggac agccaactta tctgtttttc atgatcgcag
     2041 ctccagaagg cgcaaacgac acacatttgc aagcattagc agcactatct cgattgctga
     2101 ttgatgcaga tttcgtagga aaattaaaag aagcaaatac accagaagaa gtccaagact
     2161 tgttccaaac agctgaagag caaaaagaag cagaagaaaa agcagaacaa gaaacacaag
     2221 ctgcagaagc ttctgctcat ccagaaagaa aattcgttgt tgccgttaca gcttgtccaa
     2281 cagggatcgc tcacacttac atggcagaag atgctctgaa gaaaaaagcg aaagaaatgg
     2341 gtgtggagat caaagtcgaa acgaacggtt cagaaggaat caaaaaccga ttgactgctg
     2401 aagacatcgc ccgtgcagaa ggtgtaatcg ttgcagcaga caaaaaagta gagatgaatc
     2461 gctttgacgg aaaagaactg atcaatcgtc ctgtcagtga tggtatccgt aaaccagaag
     2521 agctgatcaa tctagccctt agtggtactg caccagtatt ccatggtgat ggaaaagaaa
     2581 gcagttcaga agaaggaaat gcggatggta ctatcggaca aagaatttac aaagatttga
     2641 tgaacggcgt atcacacatg ttacctttcg ttattggtgg aggaatcgct attgcgcttt
     2701 ctttcatgat cgaccaattt attggcgtgc cacatgatca attatcaaat ctagggaatt
     2761 acaatcaagc tgctagctgg ttcaaccaaa tcggacaagc tgcatttggt ttcatgctgc
     2821 cggtattagc aggatttatt gcttcaagta tcggtgaccg ccctggtttg atcgttggat
     2881 ttgctgctgg tgcattagct aatacaggcg gtgcaggatt cttaggtgca ctgattggtg
     2941 gattcttagc tggttatgtc atcgtatttt tacgtaaatt attcaagaac ttaccaaaat
     3001 cattggatgg aattaaaacg atcctctttt atccagtgtt tggcttactg attactggtt
     3061 tcttgatgtt gctggtcaat gtccctatga aagcaatcaa tgatgcattg aacggtttct
     3121 tgacaagtat gagtggttcg aatgctgctt tacttggtgc attattagct ggaatgatgg
     3181 cggcagattt aggcggtcca atcaacaaag ccgcttatgt gtttggtaca gcaactttag
     3241 ctacgactgt tgcaactggc ggaagtgtcg tgatggcttc tgttatggct ggaggaatgg
     3301 tacctccatt ggcaattttc gttgctacac gtttgtttaa gaataaattc agcaaaacag
     3361 atcaagatgc tggtttgacg aacatcgtga tgggactatc gtttgtcacg gaaggatcga
     3421 ttccatttgc agcagctgac ccgattcgtg ccattccaag ttttattatt ggttctgctt
     3481 taactggcgg attagtcgga gctttcggga tcaaattact ggctcctcat ggcgggattt
     3541 tcgttgtatt cttactgagt cacccggtca tgtatttggt gtttattgct atcggagcag
     3601 tcgtatctgg tgtgatttat ggtttattga gaaaagctcc tgcagcagca gaagcataga
     3661 aataaaaaag aagaggctgg tttttcccag tctcttcttt ttttatagaa tcatatagaa
     3721 tcaagtagtg tagatgaata gttgttgtaa aatagttttt ccatgttcgt taaaatgatt
     3781 gcttcaaatt tgtgatagaa gatttagcct cgatttcctg cttgattctt ggcaagtttg
     3841 cttcatcagt caagataacc aaaatatctc ctacattcat gatcgtatca ccgtgagtca
     3901 agatttcctg atttcctctg cgtatagaga ttagcagcat atttttaggc caattgaagt
     3961 ctcgtaccat taatccgtct aaagaactct ctgccgttac tggaaaatca aaagttgtcc
     4021 ttgcaccttt tagtgaggcg gtctgagtat ttccaagtag acgttccaat aatgattcgt
     4081 aaattggatt accgccgaga aggtcattga cgatataagc aacaagagca gccaatccta
     4141 aaggcatcag atgattaaga gagccaacca tttctgtcac taaaataatt gctgtcagcg
     4201 gtgcttttcc aatcgctgtg aaataaccag ccatggcaat aacgacaaaa tccttgatgt
     4261 agattggatc catcccgaaa tcaaccataa tgtttccata aaagctaccg attaaagccc
     4321 ccaatgtcaa tatcggaagg aaaatccccc ctggaagacc accaccgtaa gaaatcatgg
     4381 aaaaaacgaa tcgaagaaca aacaaaccga taatcataat gattggcaag gaagcatgtc
     4441 caaaagctaa aacgatttga ttgcctccgc ctagagtact aggccataaa taaccaattg
     4501 gcaaaatcag caagaaagga acgaacccgt gaaaataagc tggtaaaggc aatcggttgt
     4561 aaaattttgg caaagccagc aagaccttct gataaataaa acctaagatg cctagaagta
     4621 ctcctaagat cactaaggaa ccataatatt tcaacggcaa agaaggaagg tcacctagat
     4681 ataaaacagg tctcaagccg aaaaagtaaa gagagacgaa gttagcagtg attgctgaag
     4741 caaatgatgt cagccaaaca agtggagaaa aggagtgatg gatttcttct aaaacaaaga
     4801 gtaatcctgc gataggagca ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa
     4861 ttaaaatctt ttcttctgaa gatggactcc gaaacaattg tccagctcct tgtccaatgg
     4921 atgcgccaag ctgtatagaa ggtccttctc tacctagaaa aaggcccgca ccaactgaaa
     4981 gtacaccgcc aatgaacttt ttccataaaa cagagaacca gttcattgat aattccccgc
     5041 gaagctgtcc ctctacttgt ggaatcccgc ttcccttgat atccggttca tttttgacca
     5101 gataacccac gaaaaatgct gcaaggatgc agcaaatagc taggccaatt aagtaaatcg
     5161 gctgttcccg actcatttca tagatacttc gaactaaatc gcttagcttt tcgatagata
     5221 atcgaaacag gctaacgacg attcctgtca gcagtccaac aaata
//
LOCUS       DAYYNG010000169         4989 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000169 DAYYNG010000000
VERSION     DAYYNG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.171"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHBFA_002584"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACHBFA_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9395999.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHBFA_002585"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACHBFA_002585"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9396000.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACHBFA_002586"
     CDS             complement(2169..2906)
                     /locus_tag="ACHBFA_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9396001.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACHBFA_002587"
     CDS             complement(2997..3524)
                     /locus_tag="ACHBFA_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396002.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACHBFA_002588"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACHBFA_002588"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM9396003.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACHBFA_002589"
     CDS             complement(4401..4886)
                     /locus_tag="ACHBFA_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM9396004.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1327 a         1116 c          852 g         1694 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcaacggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctc
//
LOCUS       DAYYNG010000170         4975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000170 DAYYNG010000000
VERSION     DAYYNG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.172"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHBFA_002590"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACHBFA_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9396005.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACHBFA_002591"
     CDS             complement(2066..3064)
                     /locus_tag="ACHBFA_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM9396006.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACHBFA_002592"
     CDS             complement(3290..3943)
                     /locus_tag="ACHBFA_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9396007.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHBFA_002593"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACHBFA_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM9396008.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1479 a         1031 c          761 g         1704 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattt
//
LOCUS       DAYYNG010000171         4959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000171 DAYYNG010000000
VERSION     DAYYNG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.173"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..1258)
                     /locus_tag="ACHBFA_002594"
     CDS             complement(275..1258)
                     /locus_tag="ACHBFA_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM9396009.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1263..1910)
                     /locus_tag="ACHBFA_002595"
     CDS             complement(1263..1910)
                     /locus_tag="ACHBFA_002595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9396010.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1995..2627)
                     /locus_tag="ACHBFA_002596"
     CDS             complement(1995..2627)
                     /locus_tag="ACHBFA_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9396011.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2643..3194)
                     /gene="coaC"
                     /locus_tag="ACHBFA_002597"
     CDS             complement(2643..3194)
                     /gene="coaC"
                     /locus_tag="ACHBFA_002597"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9396012.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3191..3928)
                     /locus_tag="ACHBFA_002598"
     CDS             complement(3191..3928)
                     /locus_tag="ACHBFA_002598"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9396013.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3925..4914)
                     /locus_tag="ACHBFA_002599"
     CDS             complement(3925..4914)
                     /locus_tag="ACHBFA_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9396014.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         1415 a         1022 c          973 g         1549 t
ORIGIN      
        1 aagtaaaaac agcttcttat gtttttaact tggttggact tattaccgca gatgcaagct
       61 tttgaacacc gtttattcgg aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc
      121 acgctctaaa cacgtataaa cggcggaagc agaagaaatt ccttcggaaa taagctgaaa
      181 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
      241 ttctgtccca gcctcttttt tgatcagcat gattttattc agttatcttc aaaatgattt
      301 ttccgatgtt tttgtttgct tccatatatt catgcgcttg tttgactgcg ctgaattgga
      361 atgtacggtc aatgattggc cgaatcttgc cattttctag aaaaggccca gctttttgca
      421 taaaagtttg tgcgagctct gctttatact gatcacttct aggtgtcaat aatgtaccaa
      481 gcagttggac acgtttccca atcaatacac caaggtctat ctcaggcacg atggtcccac
      541 ctagcatacc aatcaagacc caacgtccat caacggctat agaagctaaa ttcttttccc
      601 agtaagaagc gccgatgaaa tccaaaataa catcgactcc ccgaccttca gtgatttttc
      661 gtacctcttc ttgaaagtct tgttttttat agttgatcaa ttcatctgca ccaagttctt
      721 tacaaaaggc gagtttttct tctgatcctg cagtaacgat gattttcgcg tgagaaagct
      781 gtctagctaa ttggatcgct gctgttccca caccacttgc tcctgcatgg atcaagacag
      841 tttcgccttc ttgtaatttt ccaagccagt aaagtgtttg ataagcagtc aaaaaaactt
      901 ctgagattcc agcagcagaa acatagtcta gcgtatcagg gagaaggatt gcacgatcgg
      961 caggcatcac agcatattcg gcataaccac ctagattgac taatccgtat acttttgtcc
     1021 caggagtaaa agtggagata tcacttttat tctcaactac ctctccagct acttcgactc
     1081 ccaaaatagg gtagggaggt ttcaaagaga ctgattctct tttcataata tctgttcgat
     1141 tgacagctgc tgtatggact ttgatcagta attcgccttg ttttggttta ggaacaggca
     1201 cttcaatttg ttctaataca tctacagaac caggatgctt gattgaaatg gcacgcatgg
     1261 atctattcct cctttgccca ttcttggaat gctgtattca actctttgct accttcagaa
     1321 agtactagag tcgtacgaga aatatccgtt tcttccacta gagcggcaag tacagcagca
     1381 actgtagagc gagtgatcgt ttttgatgcc ttctcattag gcttagttaa ttgaatggaa
     1441 gtaacttctt catcgttagt caatgtgact ggttgaagaa tgacaaaatc taagttagtt
     1501 cgtttcagcc attcatctgc ataatgttta gtaatataat aatctgtcat ggactcaggc
     1561 cattttgctg gttcatctgc ataaacagca ctgaccatca gatagcgaga gacatgcgca
     1621 ttttcagctg cgatcactgt tttgattgcg ccatccaagt ctacttgcag aagattcttt
     1681 ccttgagagc cggcagcaaa aatcaataca tcgatgcctt tgaatgcttc ggtcattttt
     1741 tccacggacc aagttaaatc aaaagagaca tacgaaacat tctctttttt tactaatgtt
     1801 tgggaagcaa catctctcac ccctgcaaaa atttcatggg aacttggagc taaatctttt
     1861 atcaggtgtc ggccaatttg tccgtttgca cctacaacaa aaattttcat ttggatcatc
     1921 ctttcgacgt caaatctttc cattcaatag taacaccaaa agagaaaaaa atcagagatc
     1981 ggctacgatg actatcagtt agtaagagaa gggtgcttga agtatcttaa caagacattg
     2041 catactgctg cagtcgcaag acctgcgacg attccttcag gtataccact tgtaaaaata
     2101 accgctagaa tagctgaata aacagcattt aaatttgttc cgatgacttt ggcataatct
     2161 tgctggaata aaaagtagat catattcatc actaaaatcg tattggtcaa tgaaccggaa
     2221 agtccagcta agaataaaga aacggaatct gctttttgtt tcattagttt gcgcagacct
     2281 ttataaacga aataaggaac caccccaatc aagatgcgag gaataaacgc gatcagcaag
     2341 gctttccagc ttccatgttc tgttccgatg actgggataa acggagaaaa gacaaaagac
     2401 aaaggtgttt ggataactgt gctacggatc atgctgatca gtccaaatgt accgccaaga
     2461 aaagcaccta gtcttggtcc taaaacgatc gaagcaatga ttacaggaat atgcatggtt
     2521 gttgcattga ttggaccgat agggataaaa cctaaaggcg taacagctaa taaaatcaaa
     2581 atagctaaaa acatcgcagt taatgtaaag ttttttgtat ttttcataag tactcctttt
     2641 ttctaagcat ttctttcatt aagagtcagc atgacctgtt cgatgatcgt ctgaatatca
     2701 gctaaggcac cttttccaaa atctccacaa gctaaaagag attctcttgg ttcaatttcc
     2761 tgatagccga cagatctcaa tgtttccaga ttacgctgag tgatcgggtt ttgatacata
     2821 ttcgtgttca tggcaggggc aatcaatttt ggtgttccca ctggaagcgc cattgcgact
     2881 gttgaaagaa gatcgtctgc aatgccgttt gctagcttgc cgataatgtt tgctgtagca
     2941 ggcgcgatga gaaacaagtc agcttgtttt gctagttcga tatgattgat tacctgcgga
     3001 tttgattctt gcatgacgtc tgtatggact gcgcgcttcg acagagattg caaggttaat
     3061 ggcgtgataa aatgtgtgct gcttttagac ataatcacat ctacctgggc acctaatttg
     3121 acgaattgac ttgtaagatc agcgcttttg taagcagaaa tacttccagt aacgcctagt
     3181 aagatctttt tcattttgat tcctccagtc taattttctc agtaatcaat gcagcaatct
     3241 cagatttact ttgagcttct tccactgttc catctttaga taagagatag ccgtggtgtg
     3301 tctcgccatg gatttccgtc aaatcatttg cgaaaataaa atcagttttg ttatttatca
     3361 gactattttt tgcgactgcc agaagagatt cctttggtac atctactaat agtttgaaac
     3421 caatgatttt tgcttttggc tgccattcac gtagataaga aatgattttt ggtgttttct
     3481 tcaaaacgag cacaagatgc tcggtgttag aagagatctt tgaaacagtg gtatctgtct
     3541 gttcattttc cgcaaaccaa ttgtccagtt cttcttgcgt tgaagaagtg ggtaggtttt
     3601 ttgccagctg ttcttctgaa aaactaaaag caggagtaaa atcactgact gccatactgt
     3661 ggataatcgc atcgtagtgt tcttttttcg ttagttcttc taattgtaaa aataagtcca
     3721 gtgcagattc gatcgtataa atcgttatat tggagtgtct tttcggtttc agtgcttgtt
     3781 tcgtagtcac gtaatcgatt gtggtatccg gattggcagc taaataatca gctaatgctt
     3841 gccctaatcg tcctgtcgaa tgatttgtaa tagcacgtac ttgatcgatt ttttcagtcg
     3901 ttcctccagc tgtgattaga atcttcattt atggtcacct acatttacta gtgttcttcc
     3961 ttggtgtgtt ccagcaagta gtttagatgc tgtttcaggc aattgatcca gagtaatctc
     4021 ttggacgacc agttcatctg caatctgcaa atccgccatc tgttgccata aagaaagacg
     4081 ttgatctatt ggtacattga cagaatcaat tccaatcaac tggatgtttc gcagaataaa
     4141 aggcaagaca gtcgcgttta gtttgattcc cccagcattg ccacataaag ctactgcacc
     4201 gttataagag atcaatggaa gaagactgct tagctgctcg ccacctactg tgtcgatgac
     4261 atagtcgatt tgctgttttc ctaaagcttt tgttgtctct ggcagaaatt catcaggtcg
     4321 tacgacttgc aaagcaccta gactttttag ccatgtgaca gactcttctt ttctggaaaa
     4381 ggcaatgatt gactggaatc ctagtttatg taacagggct atcgctgtac tgcctacacc
     4441 gccagaagca ccagtaacga caactttttt atcggaggtc ataccttgac ggattaaagc
     4501 gttcacacac aacatagcag taaaccctgc tgtaccaagg atcatcgctt gacgcagcgt
     4561 catatttttt ggtagaggaa ctaaccagtc tcctggtact ttttgatatt ggctataacc
     4621 gcctggatgg gtgacgccaa gcccaaaacc agtagcaagt acaagatctt cttttgaaaa
     4681 acgtggattg ttactttgga cgacagttcc ggataaatca atccctggag tcatcggata
     4741 tgttcgtatc acctgaccag actcgctcat tgccaatgca tctttataat tgacatcaga
     4801 ataggcaatt tttaccaaga cttcattttc agccaactca gtaaatggca gatcactgat
     4861 tgctgtatgg aaaacaggtt cctttattac ctgaaaagat cgaaaagcgt tcattcgtga
     4921 gacactcctt ttaaaatttt cttttatcat accataaag
//
LOCUS       DAYYNG010000172         4936 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000172 DAYYNG010000000
VERSION     DAYYNG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.174"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..334
                     /locus_tag="ACHBFA_002600"
     CDS             35..334
                     /locus_tag="ACHBFA_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396015.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            599..1474
                     /locus_tag="ACHBFA_002601"
     CDS             599..1474
                     /locus_tag="ACHBFA_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396016.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1471..1689
                     /locus_tag="ACHBFA_002602"
     CDS             1471..1689
                     /locus_tag="ACHBFA_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396017.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1676..4303
                     /locus_tag="ACHBFA_002603"
     CDS             1676..4303
                     /locus_tag="ACHBFA_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM9396018.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4318..4536
                     /locus_tag="ACHBFA_002604"
     CDS             4318..4536
                     /locus_tag="ACHBFA_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396019.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1934 a          615 c          984 g         1403 t
ORIGIN      
        1 ttatagaata attaattaaa aaggagaggt ttttgtggaa gcaataaaat taatgatttc
       61 agaagaacag gaggaaatgt ttctaaagca gttttatcaa attgctacaa aagctattga
      121 aaaggctcaa gaaaatgcgg ggatgacaag ggaatatctc aatcaaaaag aaatggcgga
      181 atacttaaat gtgagtgaaa actttttaaa aaatgctgtg agaaatgagg gattgcccgt
      241 agttgaatta ggggcaagaa aattttattc gaaaaaggca gttaatgagt ttatgtttgc
      301 tcgaatgaaa agaggggaaa atattggaag atgatgaagt agaaattata aaaaaatgct
      361 ttaaaaaaag aaaaaaagtg ttcccaaaag tttgcagact gggggaacac tcaataataa
      421 agtgctgaga aaactttatt tatagctatt atatcaaaaa agaaggtgaa aaggtgtttt
      481 cttctggaaa tgttgtagtt gacaaactta gagaaataaa ttttgacgga aatgtaatcc
      541 acacacatgg tacgatcaat taagaaaaaa aacgaaaaaa ggtgttgaga agccctattt
      601 gaatgcaata gttatcttag cagaaattac ctattggtat agacctaaaa aaatttttaa
      661 cgatgatggt cagctacttg gttatcaaaa aaagtttctt gaagatattt tgcaaaaatc
      721 atataagcaa ttaagcaaaa aaacaggatt atcagaacga gctataaaag atgcaattgt
      781 gtacttagag aaaaagggga ttatcaaaag ggtttttaga aatatttatt atgagaaaag
      841 agcagttaca ttatcaaatg ttatgtacat tgttttaaat gtacataaat tgatagaaat
      901 tacttttcct actttggatt gcgaaaaaaa ggagtatatg ccgaaaattc gtcagagggg
      961 tatcgaaaaa aagagatatg tctctttaaa aaaaggtaga ggtatgtaca aatttgtata
     1021 tacgtataca aagactactt cacagaacat atcaaagact actctcgtta tggaagaaga
     1081 ggatattaat ataaagagga tttacagtaa gattagtgtt ctttatgacg caaataacga
     1141 agatggtcga aaattatttc aagtgtatca ctatttgaga aacgctaatg tagatacaga
     1201 agatatcaga aaaatcattg aatatttatc tgaaaatact cagtatctta ttgctgagtg
     1261 tattataaaa caacatgaag catgcttttt tcaaagaaaa actgaagaag ggttatacga
     1321 ttatgtaaca tattttttga atggacttca gaaaagatat aaaagtcaat ttttagaaaa
     1381 tgtagagcct atagatcagt tcaaaaaagc tttcgaaatc aaagagttac ctgaaattac
     1441 tatgcataat tggttgaaag gagaagaatc atgattaaaa atcgcttaat agatcaaatg
     1501 tggtgtcttg atcaaaatac agatactctc gaattaatcg agagtatctg cttcaacaca
     1561 gtagttctcg atttaagagc tgaaaatgac aattgtgtaa ctcatatcat aattaatcaa
     1621 aaaatggctc agcagctctc tgagagccta agaaaatggg ctgaaggaga aaactatgaa
     1681 agcaattaaa gaaaaaatcg ttagtagaaa aataaactgg ttagcactca gtgtacttgt
     1741 tttaatgatt agcgttgttt ttacaagtaa tattgttgaa gctagcattt tcaattggtt
     1801 tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac cttacctaaa
     1861 aaaatacaat ggagatttcc ttagtcagtt cgggatttgg cttggatggg ctgtggtaaa
     1921 aggcatgttt actgtgactg attcgattca aaatatgatt ccagatgtgt tggatttgtt
     1981 caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagtcataa atacaattgt
     2041 cgttggatta atgatccttt cattgatgtt tgttggctac aagatgatta ctggaaaagg
     2101 aacaatagat ttaagatctg ttggaatgaa tatagtgatg tctgtagcgt taattctttt
     2161 aatgccaaca atgatttcta gcggtattca atttgccaaa atattttaca atgatgctac
     2221 tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag gagtaacaga
     2281 tctggcatat attaataaaa cagatcaata ctcaagcatt gataaaacgg aggatcgaaa
     2341 taagttaaca cgaaaaaatt ttcaacagac agatttaaca caagtgttga cggataaagt
     2401 gattgataaa ttggaaaaag aaaatccagc tgcggataat cttcgctatg aattagttga
     2461 aaattctaat aatgaatttg ttgccacaaa gttttctgat aacttcttat caacattttc
     2521 tgatagcttg aaatcaggtt attatcgata tcaagcaaat ctttggggga tttcgattgg
     2581 gttgacagcg ttggcaatcg cctatgtgtt cagcgcgttt gtgatcatta cagcaatatt
     2641 agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcgacagata ttgaaacagg
     2701 acaacgttca aaagttgttt tatccgatat tttacaatgt tacttaacag ttggatttca
     2761 agggtttgga ctatctatgt ttgctatgtt tatcaatttt ttaaatgctg gacaaggtat
     2821 ctccacaaat atttttataa aaacaattgc ttatatctgt gctgtgtttg tgctgataaa
     2881 aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag aaggctatgg
     2941 gcaacttgct tctgcttttg gtatgggcgc aatgctgttt agaaaaggat caactggatt
     3001 taatcgtgct aaaggaagcg gaaatggaaa tggatcagac agtggagaag gagaagatcg
     3061 aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc
     3121 tttgggatat gcccatgaac gtggattatc aggtttagct tcagatggag ctacaatggc
     3181 tagtgaaaga gcaataaaac catttaagag tatgcgtgat atggctaatg ataccaagaa
     3241 taagttcaaa gaaggtttag atgatggtac agtgtccgct attaataaaa atagcaaacc
     3301 tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattcca tgccaacacg
     3361 tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc gatagaatta tgtctagttc
     3421 tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc ctgtaagtgc
     3481 aattcagcaa aaagttcaac aagatattga agaacgtaaa aatgctatgc acggtcaagc
     3541 taagtctgct gaagaattaa ttaatcagaa gcgacaagaa gctaaataca cgcctgaagc
     3601 aatgaatcgt gaggaaatgt taaggaaacg tgtagaaggg cgtacaggag ctaatacgga
     3661 cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaactcaa ataccggatt
     3721 agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg gccgatcagt
     3781 agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga atgttgatgt
     3841 aagagagaat ttacaaagct cttcaaatgg tcaaggtact aagacaattg atgttcgaga
     3901 aaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa gagagaatgt
     3961 acaaaattct acaagtagtc aagggtttaa atcaatggat gtaagagaga atgtacaaag
     4021 ttctacaagt agtcaaggat tcaaaacagt agatgttcgg gagaacgttc aaaaagatag
     4081 tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa
     4141 aaaatttgat tcaaatcatg aaacagtgat tattgatagt gaaatccgtg aaaatgataa
     4201 gggatcaaaa ccaagacgaa gatttaccta tgaagacaac gagttattca gagatacact
     4261 taatgatcct aatccgcttt tcgataatct attaaaaaaa taaaaggggg aattgtcatg
     4321 aaattaattc taaagattag tacgcatgat ttcttagaac ttgacgaatt atgtgaatca
     4381 gtaaagaacg caacaaatga aaaaatcacg gtcactaatt atattgaact gatgattcaa
     4441 gaagaattaa agatgaaaca tgagataaaa aacaacttga aatattgtta tctagaaaaa
     4501 agtgaagaga taatccatga attaaatggc ttttaaaaat tgtatttgac atgaatttac
     4561 gacaaggaat tagtggggat taagcgatta ttctcttcca ctttagagaa ataatgggtt
     4621 aaaaagccag aatacgtaga gcaatattga caacctataa aggagctatt gttgaaaatt
     4681 gaagtaacaa aaagataaag ggaggtttac atctatcaga ttaaaagtga tagtacaccc
     4741 atgaaacctg caaaagagtt gaacctaatg tatatcggtt taactctttt tatttgcaaa
     4801 ttaaaaaaat atcaccgaag tgacgaatga aactatgacc aaattaagca agaaaatata
     4861 tcttgcaaat aaagtagtat aaaaagatat tgataaagcc aatcaaaact ttgaatttca
     4921 aacgttagat aatcaa
//
LOCUS       DAYYNG010000173         4931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000173 DAYYNG010000000
VERSION     DAYYNG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.175"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHBFA_002605"
     CDS             85..972
                     /locus_tag="ACHBFA_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9396020.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBFA_002606"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBFA_002606"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9396021.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHBFA_002607"
     CDS             2274..2720
                     /locus_tag="ACHBFA_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9396022.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHBFA_002608"
     CDS             2993..4345
                     /locus_tag="ACHBFA_002608"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM9396023.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHBFA_002609"
     CDS             4516..4803
                     /locus_tag="ACHBFA_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9396024.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAYYNG010000174         4889 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000174 DAYYNG010000000
VERSION     DAYYNG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.176"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..1725)
                     /locus_tag="ACHBFA_002610"
     CDS             complement(115..1725)
                     /locus_tag="ACHBFA_002610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9396025.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1947..2192)
                     /locus_tag="ACHBFA_002611"
     CDS             complement(1947..2192)
                     /locus_tag="ACHBFA_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396026.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2918..4258)
                     /gene="glnA"
                     /locus_tag="ACHBFA_002612"
     CDS             complement(2918..4258)
                     /gene="glnA"
                     /locus_tag="ACHBFA_002612"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9396027.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4286..4678)
                     /locus_tag="ACHBFA_002613"
     CDS             complement(4286..4678)
                     /locus_tag="ACHBFA_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9396028.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1439 a         1053 c          798 g         1599 t
ORIGIN      
        1 ttgctttcta tcttttttcc tacaaataat aacaaatcag aaaaagagaa aagtgacaag
       61 aaaattgctt atccccttct cttgctactt ttctctttct atcaagttca aaaattattt
      121 tgtttcgtga tctgctaatg atgctccgac aaatgctttg attaattttt gcgggcgatt
      181 tggacgagaa atcaattctg ggtggaattg gcatcccaca aagaattttt tctctggcag
      241 ctctacgatt tctactaaac ggttatctgg tgagacacct gaaaagacta gcccttgttc
      301 ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg tgacggtgac gttcttgcac
      361 aacatcggct ccatcatatg cttcagccgt cttcgttcct tttttgattt tacatgggta
      421 taatccaagg cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag ccattaaatc
      481 aataatattg tttgctgtat ctggattcgt ttcagctgat gacgcatctt ctaatccaac
      541 gacgttacgt gcaaattcga cacaagccat ctgcatacca agacaaatac ctaaaaacgg
      601 aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc ccttctactc cgcgatcacc
      661 aaatccacca ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta cattttctct
      721 cgttaattct tgtgctttga tccaatcgat ctcgatgtct gaattatagg cgaaaccaga
      781 atgtttcaat gcttcaacta ctgagatata tgcatctggt aactcgacat atttaccgac
      841 aagcgcaatc cgtacttttt tcttcaagtt caagacacgt tcttctaatt ctttccattc
      901 tgtcatgtct gcttctggtg tatctagttt taaatggtcg caaacaattt gatccattcc
      961 ttgtgcttgt aaaactaatg ggatggagta aagtgtatct acatctggcg actcgataac
     1021 tgcttctggt tcgacatcac aaaattgtgc caatttgttt ttcacgcttt gagaaacagg
     1081 ttgctctgta cgtaccacca aaatattcgg ttggatacct aaaccacgta gctcttttac
     1141 actgtgctgc gttggtttag ttttcatttc tccagcagct ttcaagtaag gaatcaatgt
     1201 tgtatggata tacataacat tatctgtacc gacatctgct ttcatttgac gaagtgcttc
     1261 aaggaatggc aaagattcta tatcccctac tgttccccct acttctgtaa tgatcacatc
     1321 tgaatcagtc attttagctg ctcgcatgat tttttctttg atctcatttg taatatgagg
     1381 aataacttga acagttgctc ccaagtattc gcccttacgt tctttacgca aaacttcaga
     1441 atagattttt cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa taaaacgttc
     1501 gtagtggccc agatccaaat ctgtttcagc accatcatct gttacgaata cttctccgtg
     1561 ctgatatggg ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt tttggatagt
     1621 tacttttaag ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa tcccttttcc
     1681 gattgatgaa acgacaccgc cggtcacaaa aatatatttt gtcataagtt caaaaactcc
     1741 tttttaattt atgtgcagga aaagtctcag acaataaaca aagctcccta ttcttcaata
     1801 agaataggga gccgaaattt ctgtaaactt ctgaccctct aaaagggtgc ccaaggtgta
     1861 taatacaagc tgactaaggg aaagtcaagt actcaaatga atactatccg cttacaattt
     1921 tttctttttc gatatccttt gagaaattat ttcttaagtc ctttatgagt gcaaaaaaaa
     1981 tttaacccac ataataagct tgatataaac atagctaacg ttaaacctaa gcaaatcaga
     2041 aaatcgttac tgaaaaattt taagtgaaat aatagataaa aaaataaaac aaacattatg
     2101 atagataata tcccacctaa caaagaacta ctttttccta cttgcagcaa cagtttatat
     2161 agcttatcgt agaaacaatc tttttctttc acccttattt acctccttta cgtaaaaaac
     2221 gtagtccaca gttctcggat ttttggcata tcttttctat taaataatat caaaaacaaa
     2281 acaattccac taattttttc aaagatgacc tttttttcaa caaaagtgcc ttgtagggat
     2341 tcattgaagg taacgaatca cataagtaaa aaataaggag atgcgacaaa agtcgtgcct
     2401 ccttatttct aaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     2461 atcaaacatg agaagctgtt tttgaaatta tttcttgtga ttcggagtta aatggtaggc
     2521 tcacaacctc tggataaacg gtattcaaaa gccagtttct cggtattaag ccaaatttat
     2581 gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacgtctt
     2641 tttcaaaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcagcc
     2701 aattcaaaaa caagaaaagc tgtttttgct tggctattct tattacttgt tgcagggcag
     2761 ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
     2821 aaaacggcaa aatatgagga actatttttc cgttagtttc tctaatactc aggtcaaaac
     2881 gctgatttca cagcctctaa ttcacggtat tcctgtttta ataaagttct agatattgtt
     2941 ctctttccca ctcggaaaca gtttgtctga atgccgccca ttcgagacgt tttgcttcaa
     3001 caaaatttga gaagatatgt tgtcctaatg cgtcaatcat tacattatcc ttacgcaatt
     3061 ctttgatagc attatggata gttgaaggta agtcctggat atgtgcagct tctctttctt
     3121 cctcatccat tacataaatg ttacggtcaa cagctgctgg cggtgtgatc tgattacgga
     3181 taccgtctag tcctgcttgc aacaatactg ccattgccaa gtatgggttt gcagatggat
     3241 caactgaccg caattccaaa cgtgtggata aaccgcgaga ttctggtaca cgtactaatg
     3301 gcgaacgatt acggccactc catgcgacat aaacaggcgc ttcataacct ggaacgagac
     3361 gtttataaga attgactgtt ggattgcaaa cggctgtgta ggcacgagca tgtttcagca
     3421 aaccacctaa gaaatgataa gctgtttcac ttagttccaa ttcaccgtct ttatcataga
     3481 aaacatttcc ttcatcattg aacaaagaca tattgcaatg cataccagaa ccattgatcc
     3541 caaataacgg tttcggcatg aaagtcgcgt gaagtccatg cttgcgtgca atcgttttga
     3601 caacgagctt gaaggtttgg atattatcac aagcatctac cacatctgca tatttaaagt
     3661 caatttcatg ctgtcctggc gctacttcat gatgtgaagc ttccacttca aagcctagac
     3721 tttctaattc caacacgatg tcacggcggc aattttcacc taaatctgtt ggtgcaaaat
     3781 caaaataacc acctttatca ttcaaggtcg ttgtgatccc accgtcttcg tctaatttaa
     3841 ataagaagaa ttctggttca ggtcctaaat tgaaagaggt aaaaccaagg tcgcgcatat
     3901 ctttcaatgc tcgtttcaaa ttcccgcgag gatcgccggc aaatggtgtc ccatccggat
     3961 tatagatatc acaaattaaa cgagcaactt ttccatgagc actttcccac gggaagatca
     4021 tccaagtgga gagatctgga tacaaataca tatcgctttc ttcaattcga acaaatcctt
     4081 caatcgaaga accgtcaaac atcattttat tttctaatac tttgtcgatc tggctcactg
     4141 gaacttctac atttttgatc gtacctaaga tgtctgtgaa catcaatcgt aaaaatcgta
     4201 cgttttcttc ctcaatgatt cgtttgattt cttcacctgt tatctgtttc tttaccatgg
     4261 tatccctctt ccttttttaa tttattcagt caaagaattg tctttgcaat ccttgcgact
     4321 gataatgacc tgttttgctt aacccacctt gcgccaaaat ctcatcacga aaaatttgtc
     4381 gaacgtcatg atctgtcaac ggcttcttgt tttttgcttc ttgttttttc gcctgtgcca
     4441 tttcgtatac acgtttgatc ccagccatat tcaatccctc tgaaagatag tctttaatct
     4501 caagcaacat atcaatatca ttcagcgaat acatacgtcg gtttccttcg cttcgttccg
     4561 gatgaactaa atcttgttct tcataatagc gaatttgccg agcagacaaa tcagtcagct
     4621 tcataactgt accaattgga aatacggaca tagatcggcg caattccttt tctcccatca
     4681 gacgtcccct ttcgttttat aagttggcta aagtgtacca attaaaaaaa atattgtcaa
     4741 ctgcatatgt taggttttct aacatgagat ctctattttt tataaaataa caaaaaaata
     4801 agaacacaaa aaaggtatct agttgatttt cgcaccagat accttttatt ttttattata
     4861 ctgtactatc ctttattagt gtttttatt
//
LOCUS       DAYYNG010000175         4874 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000175 DAYYNG010000000
VERSION     DAYYNG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.177"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1246)
                     /gene="nagA"
                     /locus_tag="ACHBFA_002614"
     CDS             complement(98..1246)
                     /gene="nagA"
                     /locus_tag="ACHBFA_002614"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9396029.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1322..2158)
                     /locus_tag="ACHBFA_002615"
     CDS             complement(1322..2158)
                     /locus_tag="ACHBFA_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396030.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2342..3559)
                     /locus_tag="ACHBFA_002616"
     CDS             complement(2342..3559)
                     /locus_tag="ACHBFA_002616"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9396031.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3708..3869)
                     /locus_tag="ACHBFA_002617"
     CDS             complement(3708..3869)
                     /locus_tag="ACHBFA_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396032.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3942..4025
                     /locus_tag="ACHBFA_002618"
     tRNA            3942..4025
                     /locus_tag="ACHBFA_002618"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3976..3978,aa:Leu,seq:gag)
     gene            complement(4265..>4874)
                     /locus_tag="ACHBFA_002619"
     CDS             complement(4265..>4874)
                     /locus_tag="ACHBFA_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396033.1"
                     /translation="SSFGKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFF
                     LNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVL
                     ALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVI
                     AILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1490 a         1010 c          784 g         1590 t
ORIGIN      
        1 acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaaacct taaaaccaaa
       61 cttaagaagt ttagttttaa ggttaactta aattatatta tgcgtggaaa cgttccacgc
      121 catctagata agttgcttcc aatgtcatat cagggttcaa tacgataaag tctgcatcgc
      181 gtccttgagc gatttttcca catgtatcat cgattttaca gctgattgct ggaatcagac
      241 tagccatcat gactgcttgt tctggtgttg ctaatcccca gtctacaaca tttttaattg
      301 cttctttcaa tttcaatata cttccagcta agttgccaga ttctagacgt gctgttcctt
      361 cttttactac tactggaaat tcgcctaaat tgtaatttcc atcaggcatc ccaccggcca
      421 tcatacagtc tgtaattaat gcgacatgat catgtcctgc tttttccatt aggattcccg
      481 cagcatttgg atgtacatga tggccatcac aaatcaattc agaaaagaca tgttctaaag
      541 ataataacgc acccaccata cctggttcgc gatgattcaa tccacgcata ccattgaaag
      601 catggacaaa tacacttgcg cctgcttcaa cagctttttg cgcttcttct agtgtagcat
      661 tactgtgtcc tagagaaaca actactcctt cttcagtcac ttgtttgaca aactcttcta
      721 caccttgacg ttccggtgct aaagcaattt ttttgattaa accacctgaa gcttcttgcc
      781 attcatggaa agtatccaaa tcaggatccc cgaaataact tgggttttgt gcccctttgt
      841 gttcttctgt gaagaaagga ccttcaaaat agattccttt gattttagct cctggtgctt
      901 cctgatacat tttgccgatt gtctcagcta catcttttaa acgttctttg cttgacgtca
      961 atgttgttgg taaaaatgaa gtcactccac aagataacag accttcagac atcactttga
     1021 tgccttctgc atcattatcc attacatcat gattcatata gccgtgaata tgtgtatcta
     1081 ccaaaccagg agcaatccat ttgccgcttt ggtcaatgat gttgacttct ccctcaggta
     1141 gttctttggt gtattcacca aaaagtccat cggtaatttc aagatagcct gcatgtttga
     1201 cacctgacgc taaaaagaat ttgtcagcat agataaaagt tctcattcta ttctctccct
     1261 ttgtttttct acctctaaag tactcttatt tagagaagaa gtcaagaatg gtctacacca
     1321 tttacaaatt ttttgaaatt tgtttcacag cttgtgctaa gccttcatag aatgctgctc
     1381 tttctggttc ttctttacgc ttgtatatta agcttttaag cacaaataaa tcattgaggt
     1441 aatagtagct attcttctct tgagcccaat agattccttg attcacctgt tctaacgctt
     1501 cgtcgatttc attttgatgg aataagaaat ttccataatt ataaaaaatt tgcgaaaatt
     1561 cactttttga tcgttcatca gctgattgat gcagcagttg gatgcttcga ctcaaatagt
     1621 agcgtgcttc agccgttttt cctgccacac catatatttt tccgatacaa ctaatcagtt
     1681 gtatttctgt gtcagaaaca tggattcttt ctttttggct agagtagctt aatgcttctt
     1741 tcaaataata aagggactga gagatatttt gacgcaacgt gaattcgcaa acaccgagat
     1801 aataataata gcgttgaaag tcagttgctt gatacagatt ttcagaaata tcagaagatt
     1861 ttagtacttg ttctaacttc cgataatcac gtttttggaa acaactatcc aacagttcaa
     1921 ataatttatc gtttgtccga gtagtttttc gagagagatt catcacatga tcaatcgtta
     1981 cccctagccg ttgacaaatc tgcatcatca ccaatacatt cggcagttcc tcattatttt
     2041 cgatccgact taatacactt tgtgaacaaa tatcatgcga aagcattttt tgcgtcattt
     2101 tttgttcttt tcgaatctct ttaatgatcg ttccaaagga ctcgattaac gtttccataa
     2161 gtatcctccc caaaatatgc gtattcgcat tctattcgta ttttacttta ggaagtcgca
     2221 aacttcaaga aaagacttgc aactaaataa tatactttag ttatttaaga aaactccact
     2281 agaaagagag cctcagtgtg tttgtctgtg gccctcctac tgaaaaattt ggtttttaaa
     2341 atcatttttg gcgataggtt gctaggaatt ctctcatttt cttagctgtt tgtttcaaat
     2401 catccatttt aggaagataa acgattcgaa aatgatctgg ctggttccaa ttaaatccac
     2461 caccatgtac aagtagaata tgatgttcat gtaaaaagtc caaaacgaat ttttcatcat
     2521 ccaaaatatt gaatcgcttt gtatcaattt ttgggaatat ataaaaagca gcttttggtt
     2581 ttacggctga aagaccagga atatcattga ttgcattata gatatattct ctttgttcat
     2641 agatccttcc gcctggcagg agcagctcat cgacactttg ataaccgcct agcgcggttt
     2701 ggataatttg ctgagacaat acattggaac acaaacgcat ggaggacaac atattcagcc
     2761 cttctatata gcctttgacg tgtgacttat ctccacttag gaccatccag cctactcgaa
     2821 atcctgctac acgatgagat ttggatagac cgttcagcgt aacaacgaac aaatcaggcg
     2881 ctaatgtagc aatcggaata tgttctagac catccatcac taagcgatca tagatttcgt
     2941 cggaataaat aatcaggtta ttttgtcgag ctacttcaac gatttcttct aataattctt
     3001 ttggataaag tgccccagta ggattattag gattaatgat aacaatagct tttgtccgac
     3061 tcgttatttt tgatttgata tcatcaatat caggatacca ttctgcctct tcatcgcaaa
     3121 tataatgaac aggtgtgcct ccagctaatg caatagaagc cgtccacaac ggatagtccg
     3181 gcattggaac cagtacttca tctccattgt ccagcaaccc ctgcatacac atcgtgatca
     3241 attcgctcac tccgttgccg gtgtagatat cattgatcgt cacattcgga aaatgtttca
     3301 gttggcaata ttgctcaatt gcttttctag cagaaaagat ccccttggaa tctgagtagc
     3361 cttctgagtc acgggcattc atgatcaaat cacgaatgac ttcgtttggt gcttcaaatc
     3421 caaaaggtgc gggatttccc gtgttcaatt ttaaaatact aatgccctct tcttgcatcc
     3481 gctccgcttc ttctagaacg ggaccgcgaa catcgtaact gacaccttct aatttgttgg
     3541 atttttcaaa ttctctcatc acttagccaa tccctcattt caaaaagtta gaactttcca
     3601 aattttttgt tatttactat ttcaatatca tactccatcc tagaaaattt tgccacttat
     3661 ttaggaaaaa cgtaagtatt aaaaaaacac aaacaagcgt tgagaactta attgtcatga
     3721 tagtcattcc atagattcgc tgcccaatcc attgcaattc cacaaatgat tcctagcata
     3781 aaaccaagca aaaaacgtcc gaatgcaagt ccaatcaata cacctaagat cattcccagt
     3841 cctgaaaatt ctccgtcata tttctccatc ttaataagtc ctcccatttt tgagcttgac
     3901 aactatgtaa aacctcgcta tcattagtgt gttattatta cgcagatgtg ttggaattgg
     3961 cagacaagca tgattgaggg tcatgtgagc gttagctcgt gtgggttcga gtcccatcat
     4021 ctgcactaca actcgtgaag ttaattttgc ttcacgagtt tttttgtttg aacgtttctt
     4081 tatgccatta tatacatatc actgtatcct ttacaaaaag aacgtacaat catgtaaaca
     4141 gcctgaggtg agtatatgaa ggtcggaaac aagctactcg tagaaacttg caagtgttaa
     4201 aacaattaat cgaaaaacga gtaaaatccc tcacctaata ctaaagcgag ggattttttg
     4261 tatattatct gtttatcacc gttattataa aggtacaaat accaagcacg gttaatccaa
     4321 attgcataaa ttcatttttt tgtacactca tgcttttcat tagctcttta actgccatta
     4381 gtactaagat ggcgataaca acataaagta tatagtacgt ccgcattccg gaagcccata
     4441 ctgtgggaga aaatcccata attaaccttg aacaaaatcc cattatcatc aaaataacta
     4501 aaaaaataga agttttctta ttgtcaaaac ttaaataaat tccaacaatt aaacatagta
     4561 ataatgccaa gactactaaa gtagctatcc aagtacctgg ataaaaaagt gacagtttcg
     4621 ttcctaactc tgtaaagaga ttattcaggt tactactact ccaaatcatt gcccttttat
     4681 ttccattgac gtaagtaaag ctttgaccca ttgtattacc aaaataaata atcaggttta
     4741 agaaaaacgg taatgcagtc aatatacgca cataatagtt ttgacatttc ctatatgtta
     4801 gaaggaaaat taagaaaaat agcaataaaa ataagatatt catgtctaaa aaaaacggct
     4861 ttccaaatga tgaa
//
LOCUS       DAYYNG010000176         4869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000176 DAYYNG010000000
VERSION     DAYYNG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.178"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..371)
                     /locus_tag="ACHBFA_002620"
     CDS             complement(<1..371)
                     /locus_tag="ACHBFA_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9396034.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIF"
     gene            765..2057
                     /gene="hisS"
                     /locus_tag="ACHBFA_002621"
     CDS             765..2057
                     /gene="hisS"
                     /locus_tag="ACHBFA_002621"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9396035.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            2072..3841
                     /gene="aspS"
                     /locus_tag="ACHBFA_002622"
     CDS             2072..3841
                     /gene="aspS"
                     /locus_tag="ACHBFA_002622"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9396036.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            3950..4825
                     /locus_tag="ACHBFA_002623"
     CDS             3950..4825
                     /locus_tag="ACHBFA_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9396037.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         1565 a          878 c         1068 g         1358 t
ORIGIN      
        1 taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac cgattgtttg
       61 agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc agcttttgta
      121 gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc aatcagttct
      181 tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc gctattcaaa
      241 gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag attatgtcct
      301 ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg tccgattctt
      361 atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac caaaaagctg
      421 aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga gactggtttt
      481 tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt tgaaacttta
      541 caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa agacactctg
      601 gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag gaaagagaaa
      661 tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca tgatgaacaa
      721 gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt ttccaaagac
      781 caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt gtcgaagaaa
      841 cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg atttttgaac
      901 actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca aaagagatgt
      961 atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt acagcaccaa
     1021 tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa ccgttcaaaa
     1081 ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg ttacgtcaat
     1141 tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat gtggagagca
     1201 tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg ctagtcatca
     1261 attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc gactatcttt
     1321 taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa aatccattac
     1381 gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct ccatctatct
     1441 tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc atgctggatg
     1501 cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat tattacacac
     1561 atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca acgatttgcg
     1621 caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca cctggcttcg
     1681 gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa gtagttatcc
     1741 ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca aatatcgaag
     1801 cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc gacttcatga
     1861 accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa ttagtattgg
     1921 tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg gcaagccgaa
     1981 aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc tatgatgaaa
     2041 tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct attgcggatt
     2101 agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac aaaaaagacg
     2161 agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg ttcaaatcgt
     2221 ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc gcagtgaata
     2281 cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa atcctaaaat
     2341 ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc aagcaaaaac
     2401 cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt tgaaatatcg
     2461 ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa atgcgacaac
     2521 aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa caccttacct
     2581 agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg tccatgctgg
     2641 acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga tgaatgctgg
     2701 attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac gtggagaccg
     2761 tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag aagaaatcca
     2821 aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta tcgaagtcac
     2881 attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca gcgacaaacc
     2941 ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag aagtagaatt
     3001 caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga atgctaaagg
     3061 cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg taggacaatt
     3121 cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag gtccaattgc
     3181 taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa aaccaggtga
     3241 cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag gtgctatccg
     3301 gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt tcttatgggt
     3361 aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct ctgctcacca
     3421 tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc ctgcaaaagt
     3481 ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ctaggcggag gttctatccg
     3541 tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta caaaagaaca
     3601 aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc ctccacatgg
     3661 cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag ataatattcg
     3721 tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg atgcaccaag
     3781 tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata tagaagaata
     3841 gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc ttgatttaat
     3901 tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca tgaaaaaaac
     3961 attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta tcgtttatgc
     4021 gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt attcaagtgg
     4081 tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag gattcgaagt
     4141 accggtatca acaactttgt atttactgaa tatacctttg tttgtattgg cgtggttaaa
     4201 aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct ccctttgtat
     4261 ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt tgtttggggg
     4321 ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat cgggcggtct
     4381 agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt ctatttcgat
     4441 ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc aatatatgtt
     4501 ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt atactaaaca
     4561 aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa atgaaatcca
     4621 aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt ataagcatga
     4681 taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac ttagagaagc
     4741 aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc agatacttgg
     4801 tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa atactgctgt
     4861 agacagacg
//
LOCUS       DAYYNG010000177         4847 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000177 DAYYNG010000000
VERSION     DAYYNG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.179"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..861)
                     /locus_tag="ACHBFA_002624"
     CDS             complement(<1..861)
                     /locus_tag="ACHBFA_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396038.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLS"
     gene            complement(1069..1995)
                     /gene="glsA"
                     /locus_tag="ACHBFA_002625"
     CDS             complement(1069..1995)
                     /gene="glsA"
                     /locus_tag="ACHBFA_002625"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM9396039.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2018..3445)
                     /locus_tag="ACHBFA_002626"
     CDS             complement(2018..3445)
                     /locus_tag="ACHBFA_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9396040.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3476..4690)
                     /locus_tag="ACHBFA_002627"
     CDS             complement(3476..4690)
                     /locus_tag="ACHBFA_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM9396041.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1516 a         1022 c          835 g         1474 t
ORIGIN      
        1 tgataggtca ttgagaacaa aatttttata aacaacttcg aaatcttttc ttaacagctc
       61 catgttctca ttggtataat ttctactgtt gaacaaagaa aaaatataat agacatcttc
      121 ttcttgtaag tctagaattc ctttttgctc ttccaatcct tgacagacta attgaaataa
      181 cggcttcgtt tctgcgcttt tcttatcttc ttctttcagt ccaaggggaa atgcctttct
      241 tccgattgcg atctctattc ctcgtatgat aaaggacttt tcatcttccg aatatcttct
      301 tttaaaaaaa tgagtctcta cataatcaat caactcgtta caagcttctt ctacatacgg
      361 atttttattt tcatagcctt cccaattgac ataacgtact aaagctagca agatgttccg
      421 cgagtcaaat tctgggataa tgatttcttt atttttcaaa taatcatttt ctttaaaaaa
      481 atccaatacg atttttttga ttgtatatac tttgctcaaa gaaataaatt caagatcagc
      541 cagttctgta ctcgtgaata taccattaaa ataataactt aaaaacttta aaaaataaga
      601 ttgaccgtac agaaactgtg tgagctcaga aagagcaata ccatgtttga atacgagttt
      661 atacttatgc ttctctctta cttgttcttt tatacaatat tctagacact cggctagact
      721 ttctagatcg tgaaggatcg ttggtgtagt cacccttaga gaaatagctg tttgttcgat
      781 gtctatttct tggaattcaa ataattgttc acacagatac acttttctaa atatatttcg
      841 ctcgatgtaa ttttccagca ttcctgatac acgcctccag tttttcttat gattttattt
      901 aatcatatct gtaaatcgta tttttgtttc ctttttattt agaaaagccc tttcaaaaac
      961 caataaaaaa agaaactggg acaaaagtca aacaagctca caatttcagc ctcattgtag
     1021 gtagctttga gccgtttgga cttttacccc agcctctttc aagaatggtt attcaaagat
     1081 gtcagcgtct agttctttga ctacatcttt caacaattgg ataccagctg cgctgttacc
     1141 aaatggatct agtgccgggc tgaagacacc tatgccgtaa cgatttggca cagctgccat
     1201 caacccgccg cctacaccgc tttttgcagg tacaccaaca tggacggaaa actctcctga
     1261 ttcatcatac aaaccagcag ttgtcataat tgatttgacg attgtagcac tttcttcagt
     1321 aatgatttgt ttgccattcc aaggagcaat tcctttattt gccagcacag cagctaattt
     1381 tgctatgcct gtagcggtga cattgaggga gcattgtctg aaataagcat cgagtatctc
     1441 cggtacgtct ccttcgatca tttgattacc tttcatatag taagcaagag agcgattgat
     1501 atcacctgtt cttgattcag aatgatagat ttcttcattc aatgtgatat taggatcatc
     1561 acagatctct ttcataaatg acaagattcg ctcaaatttt tcatctgcat ctttaccggc
     1621 aatcaacgag gttgttgcaa tcgctcctgc attaacaaaa ggattcattg gatgtttgcg
     1681 atgattgatt tccatattca tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat
     1741 tgtttgaaag actttttcta ttccgttgtc tttgattgct aataacaata cgggaacttt
     1801 tgacatgctt tcgatggcaa agcgcacatc ggcgtctcct ccctcaatca gttgttttgt
     1861 atccaaatca taaatcgcca tacctaattg tttgggatcc acttcagcta atgctggaat
     1921 gtaatccgcc acttgtcctg tttgataata gtggaaattt tttccaatca attgttctaa
     1981 ttcttttact tccatttgaa taaccagctt tcatttttta tttttctgca tgctggaatt
     2041 tatgacggca tgcgtagatc gtaaatggaa taactaagac gataagataa gaaacaatca
     2101 gaatcgtttc ataaatccca tgctgtccag ctgcgatact agcaggtgga acaaatgaga
     2161 tacaaaaagc aaaaagtgaa gtcaaaagac ctaaacaagc aacgattact tttacaactt
     2221 ttccgcctgg aacctgatag gctctttcaa gactgtcatc tttaaaaacc agcttcaaat
     2281 agcccgcgaa aaacataaag tacaacatca ggtagattac aacagtcaat gacatcgctg
     2341 caagaaatga aagattattc cccccgccgc ttaatgtaaa gacggctgcc cagattgttg
     2401 caacgatccc atttgcgatc atcaaatgag caggaacacc gtgtgtgttt tcttttgcaa
     2461 aatattcagg aagtaatcct tcttctgctg ctacgtgaag tgcttttgaa ggagaggtta
     2521 cccaagaagc aatttctccg atgacaccta aacaaacaag aatcgcaaga attttcgcaa
     2581 tccatgcaag atttggatca atgtgtctta ataggaattc taacgattga atcacccctg
     2641 tattaagaga caaaccatgt tgaggtactg tagtagcgac ggtcaatccg ccaaaagtat
     2701 ctaaaatgat tgccagaata accaatacaa acattgctaa tggataatcc tttttagggt
     2761 tttccatttc atttgcatga ctggcagatg tttctacgcc catatatgct aaaataaagg
     2821 aaacaaaaac aaccagcgta ggcaatttcg aaaaatcagg aatcaatgtt ttcatagaca
     2881 gtatcgtttg tagtggagcg cctgaagcga cataatggat ccctaagcca aacaagacta
     2941 atgctggcaa caagatacca gctacaaaac cgatccgagc aatcaatgaa gtgtatttgg
     3001 ttccttttaa ctgtgagagt aaaatagccc agaaaataat caataggatt gccaatttca
     3061 gatatttatt gttttgtata gcaccaatac ccgttgcata ggataatgca ccagttaaga
     3121 aatacagcat ggtcaagtag ccaactgtta tctcaaacca ttggaaaaag attgcggcaa
     3181 aaccgaatcg ttttcccaat gtgcgactga cccaagtata gacaccgcct ttttcccatc
     3241 ctttcaccgt tgccatctct gctgcgcaca atgccacagg gataaaccat agcaaaccgc
     3301 ctaataacaa gaaaaatact aagctgaagc ctgaggtcgc gaatgtggga tattcatata
     3361 atgaaacgac cattgagatg gtcatagcca aaaagccaaa gaacgttaat ttttttgcag
     3421 gaattttcgt tttgctttgt tgcatgaatc ttcctctttt ctatttgaat ttgtttcaat
     3481 tgagtttgtt gccaaagaag aaaaagtcca atccttcttc ttgttcttct tttgtagtag
     3541 tgatcgttgt aaagtgcatc gccgatttta aaatcactgt tgtcagcaca taggcataca
     3601 cggcagtaat gatgacacca atcacttgaa tccctaagaa gtaccagctg cttggattcg
     3661 ttagtaaatg gctgttagat gcaaagatcc ctatcagaat cgtacctgtg aatccgccca
     3721 tgccatgaac accccacaca tcaagtgcat catcccattg tttcttttta cgaaaatcaa
     3781 cagcagcatg acagacgata cccgcaatga tcccaatcag catggcactt tttgcttcta
     3841 cataaccggc acaaggcgta atggctgcca atccagcaac ggatcctgtt agtacgtcga
     3901 caaaatcaaa gccatttcct tttacttttg caatgatcag ccaagtaatc atcgcaaatg
     3961 caagagcgat gaatgttgaa ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac
     4021 caccggaatt aaaaccgaac cagccaaacc aaagcagtcc tgtaccgata gcagctgcca
     4081 taagactgct atgctgactt ctttctccta aaggaatctt tctttgacca agtgtgagga
     4141 tacatgctaa agagccgaat ccagctgtcg tgtggatgac tgctccacca gcgaaatctc
     4201 taaatcccat ctgatccaaa aatccgccac cccatatcca gtggcaaaca gggaaataaa
     4261 tcagcagatt ccaaaaaata acaagtataa tatagccttt taaattcaat ctccctgcaa
     4321 aagctcctgt catcaaagga acggtaatca cgataaacat cagttggtaa agaaagaaca
     4381 ttaaaaatgg aatcgttgca ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca
     4441 aaaagaaatc tgccgggttt ccaataacac cgccgatatc ccgtccaaag gccaatccga
     4501 atccaccaaa tacccacata atcgttgtta tcccaacgga gagaaaagat tgagacatga
     4561 tcgttagtat attattcttc cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca
     4621 tcaggcaaac cattgctgta cataaaatca tgaatgctgt atctcctgta ttgataggta
     4681 atgtattcat ttttttcgcc tcctttctac gattacgagt ataaaagaag gtgtagttgt
     4741 ttttttcttc tgtttttttg aattaatact tgcatttata aaagacagac aattaataaa
     4801 agcttgatgt gttgtgctct ttttcgtttt tcacacaaat aaagaca
//
LOCUS       DAYYNG010000178         4786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000178 DAYYNG010000000
VERSION     DAYYNG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.180"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHBFA_002628"
     CDS             <1..813
                     /locus_tag="ACHBFA_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM9396042.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHBFA_002629"
     CDS             786..1229
                     /locus_tag="ACHBFA_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396043.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2401..3369
                     /locus_tag="ACHBFA_002630"
     CDS             2401..3369
                     /locus_tag="ACHBFA_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9396044.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="ACHBFA_002631"
     CDS             3379..3849
                     /locus_tag="ACHBFA_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9396045.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3865..4035
                     /locus_tag="ACHBFA_002632"
     CDS             3865..4035
                     /locus_tag="ACHBFA_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396046.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            4445..>4786
                     /locus_tag="ACHBFA_002633"
     CDS             4445..>4786
                     /locus_tag="ACHBFA_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM9396047.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         1825 a          778 c          789 g         1394 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcgattat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agccttaaaa agagcttttt acctagacca atccaaaatc
     4261 acaaagtgag cctctgtatc ggttgataca agggctactt ggcatagtcg acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DAYYNG010000179         4781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000179 DAYYNG010000000
VERSION     DAYYNG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.181"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..492
                     /locus_tag="ACHBFA_002634"
     CDS             <1..492
                     /locus_tag="ACHBFA_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396048.1"
                     /translation="GQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENF
                     IRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRS
                     LEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKK
                     HIS"
     gene            713..2476
                     /locus_tag="ACHBFA_002635"
     CDS             713..2476
                     /locus_tag="ACHBFA_002635"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM9396049.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2560..3018
                     /locus_tag="ACHBFA_002636"
     CDS             2560..3018
                     /locus_tag="ACHBFA_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9396050.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3127..3810
                     /locus_tag="ACHBFA_002637"
     CDS             3127..3810
                     /locus_tag="ACHBFA_002637"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9396051.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3957..4571
                     /locus_tag="ACHBFA_002638"
     CDS             3957..4571
                     /locus_tag="ACHBFA_002638"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM9396052.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4587..>4781
                     /locus_tag="ACHBFA_002639"
     CDS             4587..>4781
                     /locus_tag="ACHBFA_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396053.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1694 a          768 c          991 g         1328 t
ORIGIN      
        1 ggacaagtat ttttaccgga aaggatctat cttctatcag aaaaagaaca acttctgtac
       61 ggaaaaattg tagaaatatt ggaagagtgg aaaaagaaaa atcaaatcca actaagatgg
      121 aatgaaaatt tcattcgaaa attcgtcaaa agtctttctt tgatcaacag tgaacaagcg
      181 gggagacagg aaatagaaat ttttgttgtt tcagaaagtt cagtcaaaca atttttttat
      241 cgaacccaat ttcatttgta tgcggaagaa ggcatcgctg aaataaatcc tattttattt
      301 cgttcattag aagaattgcc tgatgaatgt ctatgtgcga aaaaaagagt ggttttgtgc
      361 gatgctgcta gctatcaaaa tggattagaa actgaaaaga caaagatcta tccaatctca
      421 ttaaaaggtg caaacatcca tttgtatcat attgctcagg aacttcacct attaaaaaaa
      481 catatttctt gatgagacct taattaggag accttctaca aagcttttta attgtctgta
      541 gaaggttttt atttgagtcc tgattcgtgc gaagatctac ttatataaca agctttctct
      601 gaaatttttt taaaatgcta ttgacttaga gtcaactcta agtagtagaa tagataaagt
      661 gtaacaaatc tattttcatt aaaagaagat agtacggagg agagaatgta ttatgagtac
      721 agtattacat gtattggatg caatcaatag ccccgcagat gtcaaaaaat tatctgttga
      781 ggaaatgaaa cagttggcag aagagatgcg ccaaaaaatc ttgcaaaagg acagcgatgt
      841 aggtgggcac gttggtccga atctaggtgt agtagaatta acgattgcgt ttcattatgt
      901 atttgattca ccaaaggata aaatcgtttg ggatgtttcc catcagtctt atggtcataa
      961 gcttttaact ggtcgaaaaa atggttttga aaaaggacat taccgtgaag tgagcggata
     1021 ttccaaccaa catgaaagta aacatgacta ttttacagtg gggcacacat ctacatctat
     1081 cagcttagca acaggtttag ctaaagcgag agatctgaag aaagaatcag gaaatgtggt
     1141 agcatttatc ggtgacggct ccctaagtgg tggcttagct tttgaaggat tgaacaatgg
     1201 tggtgtttta caatccaatc taatcgtatt agtaaacgat aatgagatgt ctattgatga
     1261 gaatcatggc agtttatata aaaacttagc tgagcttcgt caaaccaacg gtaaaagcag
     1321 caataaccta tttaaagcaa tgggatttga ttatcgttac gtggataatg gaaacgatct
     1381 ggaaacggtg atccgtttat ttgaagaagt aaaagatatt gatcatccga ttgtacttca
     1441 cgtccatacg gaaaaaggtc gcggttatca gccggcaatc gataataaaa tgaaatatca
     1501 ttggcatgta ccatttgatc tgtccaccgg ggaggcaaag caagtaactt ctggagaaac
     1561 atttaatatg atcatcatgg attatttgga taagaaaatg caaacaagta cagctcctat
     1621 tgccgcagtc aatgcagcga taccaggcgc ctttggactg aaaacattcc aagaaaaaca
     1681 tccagatcat tattttgacg tggggatcgc tgaacagcac tctatcactt tcgcttctgc
     1741 tttggcttct cagggagtac gtccagtcgt cttccaatca agtactttct tgcaacgggc
     1801 atatgatcaa ttatcacatg atttagcgat caacgagaat ccagtgatta ttttagtcgg
     1861 tggcggaacg atctcagcta gtgaccgaac acaccaagga acatttgaca ttccttactt
     1921 atcaagtatc ccgaacatca aatatcttgc tccagcaaca aaagaggata tgatctctat
     1981 gctagattgg gcgttcgaac aaacagcgca gcctgtagca atccgaattc ctacacatgg
     2041 tgtagaacat ggtacagcta tcgcaaatga ttatagtatt ccagcctatc aaacagtaaa
     2101 taatggagga aaagtagcaa ttttagcttt aggcggtttt cttgaattag gaaaagaaac
     2161 agtaaaagct tttagaaaaa aaggaatcga agcaacgttg atcaatccag gaacgatcac
     2221 agaaaatgac gtagaaacat tagataaatt aaaagaaaca cacgaattag tcatcacatt
     2281 agaagatggt agtttgtcag gaggatttgg cgaaaatatc agtcgttatt atggtgattc
     2341 ttcaatgaac gtattaaatt ttggtgcaga gaaagaattc acggatagcg ttcctgtagg
     2401 gcagctttat gaacgttatc atttaacacc aaaacaaatc gttgaagata gtctgcgtgt
     2461 cttgaatatt caataattca ttatgctcac catccagata attgtttata ctagtatctg
     2521 gatggttttt tcattataaa aagcaaaggg gtggatgtta tgtatcagca agtagaagaa
     2581 tatgtcaaaa agttccaaga aaaatatcct agttatcaat cagaaaaatt aggtttcttt
     2641 tcatttgggg atacagaaaa aatggcagac gagttgagcg aactagttgt aaaaaagata
     2701 aagactggta catcaagtgg ttacgatctg tataaacaag agaatgagcc tttaccaaaa
     2761 gttggtcaat tagacgtggt cctgaatgga aaaaatgaac cagtttgtat catcaaaaat
     2821 ataaatgtga ccatcttgcc ttttagtgaa gtcacgaaag agcatgcttg gaaagaagga
     2881 gaaggggatc ggacattagc ttattggcgg aaagtccatg ctgagttttt taaacatgct
     2941 tataaagaag cgttgaacat agaatttaca gaagagaagc tggtcgtata cgaagaattt
     3001 gaagtaattt tcttttagta agaaaggtct gctgaaagaa atagctcatt attgtctcct
     3061 gaatagaaaa gggttacatt tttatatgaa gctgtatttt tatataacat tactggagga
     3121 gatattgtgg atacactgga acaaatcaaa ggcggagtcg tcgtttcttg tcaagctttg
     3181 gaaaacgagc cgctacacag ttcttttatt atggggagaa tggctttggc tgcaaaagaa
     3241 ggcggagcag ttggtattcg agcaaatacg acagaagata tcaatgaaat caagcgaatc
     3301 gttgatttac cggtaatagg gattatcaaa agagattacg atgggtcaga agtgtttatt
     3361 actgcgacag aaaaagaagt ggaagaactt ttggagacgc aagcagaaat cattgcactg
     3421 gatgcaactt ctcgtaaacg tccgacagaa aaaacaactg ctgaacttgt ccaaatgatt
     3481 cacgaacacg gtcggttagc tatggcagat atttcgactt ttgaggaagc gcttcaagcg
     3541 caagaagatg gttttgatct tttgtctacg actttagcag gctatacaga atattcacga
     3601 aagacgcaga cacctgattt tgatttattg aatcaaatca tagaacaagt cgatatccca
     3661 gtaattatgg aaggacatac tgactctcct gaacaagtag agaaagcata tgaattaggt
     3721 gctttttcag tcgtagttgg atctattatc acccgtccgc aattgatcac taaaagatat
     3781 gtcgaagccg caagtaaagc aaataactaa aaaacacgca cggcacagat tcttgacaaa
     3841 aaagaaaaat tgatagatag tttaaagcag attctaagaa gatgaaaaaa gcattacttt
     3901 tcattttgtc ttcaagatct gctttttaac gaattttata gaaagaaggc gaaaccatgt
     3961 ttgaacggag tccttcagaa ttgacagaac gtgaaaatta caagttgctg attggttcag
     4021 tcatccctag accagtagca gtagtaacga ctaaatcagc aaaagatata gtgaatatcg
     4081 cacccttcag ttacttcaat atcgtcagtt cgaatcgacc catcttatcc ttggcgatcc
     4141 agagaaaaca agatgcagta aaagacacag caaagaatat tcttgagaca agagaagctg
     4201 ttatccatat ccttgatgag gacaacgtag aagaagtaaa taaaacagca gccaatctgc
     4261 ctaatgagga aagcgaactg tacctgactg acttgacttt aactgattca gtaatcattt
     4321 cagtaccagc attgacagaa gcaaaagttc gttttgaaac atccttatat gaacatgtcg
     4381 agatacggaa taaaaataaa gtaacagctg atttattttt gctagaagta cagaaatatc
     4441 atttaaatga aacagtctat gatgataaga caggaaggat cgatccaaga aaattgaatg
     4501 ctgtaagccg tttggcagga aatgattatg cagcaatagg agaaattttc acgattgcac
     4561 gtcccgaata aaaaggagca ctagttatgt taaaaataag ctataaacca tcgacagatt
     4621 caaaagaaat gaaaaaagaa tatgaaacag tgaatgattt tctgcaaggg cagtacttag
     4681 aagttccgcc attgcaagac cattttgtcg ttactactgt tactttagat ggtaaggaaa
     4741 tagaaatgcc agatcaaacg attagcggat tgtttaatta c
//
LOCUS       DAYYNG010000180         4739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000180 DAYYNG010000000
VERSION     DAYYNG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.182"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            478..2358
                     /locus_tag="ACHBFA_002640"
     CDS             478..2358
                     /locus_tag="ACHBFA_002640"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9396054.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPLTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDDVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVVIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2437..2664
                     /locus_tag="ACHBFA_002641"
     CDS             2437..2664
                     /locus_tag="ACHBFA_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM9396055.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDKVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2669..3211
                     /locus_tag="ACHBFA_002642"
     CDS             2669..3211
                     /locus_tag="ACHBFA_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9396056.1"
                     /translation="MNIVEEKFAQEIKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLLASTILENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3456..4175
                     /locus_tag="ACHBFA_002643"
     CDS             3456..4175
                     /locus_tag="ACHBFA_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9396057.1"
                     /translation="MKKRILLIMGLAFLLAGCTNGNEPRKESTKETAKTTIATTTTTS
                     TESKSTATSTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KAAQLEAFMTTWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFDQIVYGQVNQ"
     gene            4505..>4739
                     /locus_tag="ACHBFA_002644"
     CDS             4505..>4739
                     /locus_tag="ACHBFA_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9396058.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPL
                     LVFFLTMASIAKHKIGNKTFVKPILVLYLVGTLL"
BASE COUNT         1578 a          775 c          989 g         1397 t
ORIGIN      
        1 tttaagttca tgaccgttta tcaatgtacg ctcaattaga cacgctgtat gaatcaaaag
       61 ttttaaaaga atggaatgag tgaatttttt gtggaaagta cattccaatt cttggataaa
      121 atccattaag cttttttgga tcttattccc agctaataag tagagatatt tgtccactgc
      181 ttcaattccc atatcaaaaa acgatatttc ttgtgaatga gtggctgctg cccttttttg
      241 attgttgttc ttcagtcgta agatgacaaa gcatcttatc tttgaataaa aaaatagaac
      301 tacctaagga agcgtttcgg cgcttccttt ttctatatga tcgtttaatg actgtaggaa
      361 ttcagtatac caaaagaaaa ccaccttttt gactaaactt gatagtggtc aattttttat
      421 gatggataag tatctatact tagtctcgta agaatgaaaa ggaaaagagg gagaaagatg
      481 aaatttcaaa agtggatcca agagcatcga aatcatatca ccgccttatc agctgtgttg
      541 attatttttg gttttatcgg aaaatttact ggatttgtct tgttctataa cattgctcta
      601 gccatagctt ctattattgc ggtcatacct attatgatcc atgcgtatca ggcagtaaaa
      661 gtcaaagtta tctcaattga tttactcgtt actattgctg tgcttggtgc ctttatgatc
      721 ggagaataca aggagtcagc tatcgttaca ttcttgtttt tgtttggtag ttatctggaa
      781 caaaaaacat tgaccaaaac gcgggaatca atcaaggctt tgaccgagat ggccccattg
      841 actgccagcg ttgtccaaga agatggaacg actgaagaag tggaaatcga tgacgtggaa
      901 gaaggtgata tcgtcttagt aaaaactggt ggagctattc ctgtggacgg acgagtcatt
      961 gacggaaaag ggtatgcgga tgaagccgca gtgacgggtg aatcacgaca agtcgtcaaa
     1021 atcagtggag atgacgtatt ttctggaacg attttagcag atggctacgt gacgatcgaa
     1081 gcaactaagg tcggagatga tacgaccttt gccaaaatta tcgaattagt cgaagaagca
     1141 caagatacca aatcacatgc ggaaaaattc attgatcgtt tttctcagta ttatactcca
     1201 accgttttac ttattgcgtt gcttgttggt ctgatcagtc gtgattttcg tttagccatc
     1261 actgttttgg ttcttggttg tcctggcgct ttagtcattg gtgcgcctgt ttcgaatgta
     1321 gcaggaatcg gtaatggagc gaaacggggc atcttagtca aaggcggtga agcaatggat
     1381 actttttcca aaatcgatac ttttgtcttt gataaaaccg gtacattgac caaaggaact
     1441 actgctgtat ccactatcaa gaactataca gaggatgaag gggacagctt agctttagtg
     1501 gctcgattag aaacgttatc cgatcatcct ttggggcgcg ccgtgatcca ttatgctcag
     1561 gagaaagaca ttgattttca acaattagca gtcaaagata atcagacgat caagggacaa
     1621 ggaatcacag ctgagattga tggtcataca gtttgtgcgg gcaacaccaa attgattgcc
     1681 gctcacaaac tttctctcac accaaagcaa gtacaagatt tacatcaatt acaacaaaca
     1741 ggcagctcgg tcattattgt agtaatcgac aaaaaaatca ctcaaattat tggtgtgtca
     1801 gatgtgattc gaccagaagt cgctgaacaa ttagctatgt tgaaacaagc aggagcaaaa
     1861 catttagtga tgctgacagg tgataatcag atgactgctg attatgtagc tgatatgtta
     1921 ggcattgatg aagtccatgc tgaattatta ccagatgaaa aggttcaatt tgtgaaaaaa
     1981 taccaagaac aaggattgcg cgttgctttc attggagatg gaatcaatga ttctccctct
     2041 ttggctgcgg ctgatatcgg gattgctatg ggatcaggaa cggatgttgc tatcgaaacg
     2101 agtgacgttg tcttgatgca atctagtttt ggatcgttgg ttcatgccta ccgtttggct
     2161 aagaaaactg ttttgaatac ccgtgaaaat gtagtgattg ccattggtgt cgtcttattt
     2221 ttactcatcg gcttatttgc cgggtttatt tacatggcta gtgggatgtt cgttcacgaa
     2281 gctagtatct tgatcgttat ctttaacgcg atgcgtttat tgtattttgg acacgaaagc
     2341 aaagaggaaa aatcgtaaaa cttgataaga atcaattttt ttatggaagc tgtaatctag
     2401 aataagacta tcaagaaaaa gggaggaaga actataatga aaaaaatgat tatgcaatta
     2461 gatacactag cttgtccaag ctgtatgcaa aaaatcgaac atgcggtttc gcaacagcct
     2521 ggggtaagca atacgaaagt tttgtttaat gcgagcaaaa tcaaagcaaa ctttgatcca
     2581 gataaagttc accctgaaca attagctgca gttgtggaaa aattaggtta tgaagtccaa
     2641 tcaacaaaag taaaagaggc ataaatcaat gaatatagtg gaagaaaaat ttgctcaaga
     2701 aataaaacaa gcagatatcg accatcataa accaactgct ggcgccatgg tggggcatgt
     2761 attagcaaat ttcaaattag aagaaatgaa attgatccaa gcacaattgt atgttttggg
     2821 tacatcttcg caagaattaa gtaccctttt ttcagaatat gctgatcaag aagcgagatg
     2881 gttcaataaa atctctcatg agttagtgat tgagaatgaa atcgttccgt ctactttaga
     2941 agaagtcatg cgttatggaa aaatttacga acatggtcaa aacaaatatt tactagcctc
     3001 aacaatattg gaaaactttg caaaagattt tgatttccaa aatgtcttta tcacacgggc
     3061 aatcaagtta gctgaaaaag aagaaaaata tggcttgcaa caattactcg ttgaactttt
     3121 aagtttcaat aaagaaatca tctaccaagt acaaagactc caaggaaaaa ctgtccgaca
     3181 agatttggat gaggaagacg atgatgacta aaagtctatg tttcttcaaa cacaaatata
     3241 aagcgtgaga aaagcaggaa aaaaacacga agtttttgtc ttaaaaataa aataaaaaga
     3301 agcacccgct gatgttgtct ataagacatc agcgggtgct tcttagtttt taaatggttt
     3361 aaaagatgga tgagaaaata caaaaaatca ataaaatcac attgcttatt tgatagaata
     3421 aagaagtggg ggagtaatcg aaaggggttt tttaattgaa aaaaaggatt ttattaataa
     3481 tgggtctagc ttttttgtta gctggttgta ccaacgggaa tgaaccaaga aaagaatcaa
     3541 caaaagaaac agcgaaaaca acaattgcaa ctactacaac aactagcaca gagagcaagt
     3601 caactgcaac aagtacagaa acagcagata cctctacttc gtcagagtct tccaaaacga
     3661 ctgaaacaac tcaaacatct acttcaagtt cttctgtaga ggcttcatct tcctcttcag
     3721 aaagcagcga acaagtcgaa caaacactgt ggaatgcaga caaagccgca caattagagg
     3781 catttatgac cacatggggc caaacaatgg gacaacaata caaaagttat acgaatcaaa
     3841 tgagtgtgga cttatatggt cttaaagtcc ctcaagctat tttgaatggg gagtggaaga
     3901 tggctattgg tggagtgcct gtttctgcag aatggtcaga aagcgggaca ggtcaagcag
     3961 attatcaaat cacagccgtc tattccgatg cagagacaga accatatctg aaaaagcacg
     4021 tctatctatt cggttttcag caaaatcagc caaaagtatt ggtgacacaa caaaatcagg
     4081 ggaatcccga taattatctt tacttcaacg agacagcgaa taatgaattg aaaaatggct
     4141 ttgatcaaat cgtttatggg caagtaaacc agtgagagtg agagaaagaa gagactcagt
     4201 ggaaaatatt aattgattgt tatgtcaaag aattaagatg aaggatgaaa tttctagttt
     4261 cgtccttcat cttagttacg ttttacttgg ataactttaa taataacgat aaatagtttt
     4321 ctctcgactt ctttagagat tgaataaacc ctccaatact aacattgaat gttgcaagag
     4381 catgaattgc caattgtatt attagaattt ttagaaaatt gcacctttag tttctttaga
     4441 taaaatgata caatgattgg taaaattgtg aaagggtggt catttgttag agaagttttt
     4501 ggatatgtcg ttagttcaac gtattgttgt tggtatttta gtcggaatat tgtttggtat
     4561 ctttttcccc acgtggacat ttatttcaat attgggtacg ttgtttgtca gttgcttgaa
     4621 agcaattgcg cctcttctag tttttttctt gacgatggct tcaattgcca aacataaaat
     4681 aggaaataag acatttgtga agcctatttt agtcttatat ttagtaggaa cattgcttt
//
LOCUS       DAYYNG010000181         4707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000181 DAYYNG010000000
VERSION     DAYYNG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.183"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(273..1346)
                     /locus_tag="ACHBFA_002645"
     CDS             complement(273..1346)
                     /locus_tag="ACHBFA_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9396059.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(1343..2170)
                     /locus_tag="ACHBFA_002646"
     CDS             complement(1343..2170)
                     /locus_tag="ACHBFA_002646"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9396060.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(2171..2977)
                     /locus_tag="ACHBFA_002647"
     CDS             complement(2171..2977)
                     /locus_tag="ACHBFA_002647"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9396061.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(2989..4074)
                     /locus_tag="ACHBFA_002648"
     CDS             complement(2989..4074)
                     /locus_tag="ACHBFA_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9396062.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(4091..4633)
                     /locus_tag="ACHBFA_002649"
     CDS             complement(4091..4633)
                     /locus_tag="ACHBFA_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM9396063.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         1436 a         1017 c          770 g         1484 t
ORIGIN      
        1 cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt aactttttca
       61 atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc cacttttctc
      121 tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt ttcatccgct
      181 tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc tttagaacgt
      241 tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt gaattctaag
      301 aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac ttcaagatgg
      361 gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt tggcaataac
      421 gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg tgccgcattt
      481 tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt cacggttttt
      541 ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac ataatgtaaa
      601 tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc cacggaggct
      661 tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac atttggtgtt
      721 aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact attcaaagaa
      781 tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat cagcatcaaa
      841 ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt tgcatcaaag
      901 aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga atactgatta
      961 tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc tttgatcttc
     1021 gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat atattcactt
     1081 ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat cgcttcatta
     1141 gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt cgtgatcaaa
     1201 ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc actcatgcca
     1261 cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat gagaatacct
     1321 aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt actcttctgt
     1381 ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca atgaccaata
     1441 tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg attccttgtc
     1501 ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg acgaaaaatg
     1561 taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct atgattcctg
     1621 gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca ccaaggtctc
     1681 tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc atcaagacga
     1741 cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct aatccgaatc
     1801 ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga gagactaata
     1861 agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg taatagattc
     1921 ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa gccagcatga
     1981 acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca taattttccc
     2041 aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa aaaatcagat
     2101 agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac gaccatttaa
     2161 attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc atcacgatga
     2221 acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt tgtgtaacca
     2281 agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca atcagacgag
     2341 tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt ttcacaccat
     2401 ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg cctaaatctc
     2461 tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt ggcataatca
     2521 taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg aataatatct
     2581 gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc acactgaaaa
     2641 tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa ataatcagca
     2701 tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg taactgatga
     2761 tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc atagaaagcg
     2821 atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca ttcatatcaa
     2881 aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg agccacaaaa
     2941 tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct attcttcgta
     3001 gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga catggatatc
     3061 ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg ttgaatggac
     3121 catccattca ttttgctgtt catcataaca gatgatttca taatggacac cacgaaacag
     3181 ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg tcaaatcctc
     3241 tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga cacattcaaa
     3301 ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca cgatattgct
     3361 ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat ctacgggtgt
     3421 accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca ttgccaacgc
     3481 ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt gcagatcccg
     3541 cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg gttcatctaa
     3601 tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct gtctttgacc
     3661 accagacatt tcactaatct cccgtttttc gtatccagga agctggacca tacgcaaagc
     3721 atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca atccaaaagc
     3781 aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga ctgtatttac
     3841 ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga tgtccccttc
     3901 tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac agccggaagg
     3961 accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt ttaatactgt
     4021 ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc ccacgttttt
     4081 accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt cgttttttga
     4141 gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt cctgcttttg
     4201 ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca aaaataaacg
     4261 tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa gtcagcaaga
     4321 caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca tacccgtttt
     4381 cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga ttcgctgctt
     4441 cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa aagaatgttt
     4501 ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt gacaaatctg
     4561 tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt cttaacttct
     4621 caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt cctgtttact
     4681 accactcgta ttagtatacg aaattta
//
LOCUS       DAYYNG010000182         4621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000182 DAYYNG010000000
VERSION     DAYYNG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.185"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..959
                     /locus_tag="ACHBFA_002650"
     CDS             153..959
                     /locus_tag="ACHBFA_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9396064.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            977..1684
                     /locus_tag="ACHBFA_002651"
     CDS             977..1684
                     /locus_tag="ACHBFA_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9396065.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1681..2652
                     /locus_tag="ACHBFA_002652"
     CDS             1681..2652
                     /locus_tag="ACHBFA_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9396066.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2645..3673
                     /gene="vanB"
                     /locus_tag="ACHBFA_002653"
     CDS             2645..3673
                     /gene="vanB"
                     /locus_tag="ACHBFA_002653"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9396067.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3679..4287
                     /locus_tag="ACHBFA_002654"
     CDS             3679..4287
                     /locus_tag="ACHBFA_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9396068.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4353..4526)
                     /locus_tag="ACHBFA_002655"
     CDS             complement(4353..4526)
                     /locus_tag="ACHBFA_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396069.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1300 a          973 c         1230 g         1118 t
ORIGIN      
        1 attttgaccg acaggtataa ccctgccggt ctttttttgc tgctacagga aaactacaga
       61 ctggctacag ggaaagtaca gatgcgcttg ccataataac aatcgtacca gccacaaatc
      121 gtagttttat tgcaaaggag gcattcgatc aaatggaaaa aagcaactat cattccaatg
      181 cggatcatca caaacggcat atgaaacaat ccgtggaaaa gcgggctttt ctatgtgcgt
      241 tcattatttc gttcacagtc tgcacgctgt ttttggggtg gagactggct tccgtattgg
      301 aggcaacaca gataccgccc atccctgcaa ctcatacagg cagcagcact gacgtagtgg
      361 agaatttgga ggaaaacgct cttgccaccg ccaaagaaca gggagatgaa caggaatgga
      421 gcctgatttt agtgaacagg cagaacccca tccccgcaca gtacgatgtg gagcttgagc
      481 aactatcaaa tggtgagcgg atagatattc ggatttctcc ctatcttcaa gatttgtttg
      541 atgccgcaag aactgatgga gtttacccga ttgtcgcatc cggataccga acaacagaaa
      601 aacagcaaga aattatggat gaaaaaattg ccgaatataa ggcgaaaggc tacacctctg
      661 cacaggctaa agcggaagca gaaacttggg tggccgtgcc gggaacgagc gagcatcagc
      721 ttggtcttgc tgtggatatc aatgcggacg gaattcattc aacaggcaac gaggtttata
      781 gatggctgga tgaaaacagc tatcgttttg gttttattcg tcgctaccca ccagacaaga
      841 cagagataac cggtgtgagc aacgagccgt ggcattaccg atatgttggc atcgaagctg
      901 ccacagagat gtacaaccaa ggggtttgcc ttgaggaata tttaaaacca gaaaaatgag
      961 aaaaggatgt tatgttatgg acagaaaaag attgacacag cgtttcccgt tcctgcttcc
     1021 aatgagacgg gcgcagagaa aaatgtgctt ttatgcggga atgagatttg acggctgtcg
     1081 ctatgcgcag acgatagggg aaaaatcgct ttcccatttg ctctttgaaa cggattgcgc
     1141 attatacaac cacaataccg gatttgacat gatataccag gaaaacaagg tgttcaactt
     1201 aaagctggca gcaaagacct taaacggctt attgataaga ccgggggaaa ccttttcttt
     1261 ctggttgttc ctgcatacgc cattgacaat tatccagcgc agaggtcacg aagtaaagga
     1321 gtttccagag ccaaacagtg acgagattaa aggggtggat gcaaccattt cagagggctg
     1381 gattgattta aaagtgcgaa acgataccga ctgcacctac caaatatggg tgaccttaga
     1441 tgatgagaaa atcatcggtc aggtgttcgc cgacaaagag ccccaagcat tatacaaaat
     1501 tacaaatggc agtatccagt atgtccgtga aagtggcggg atttatgaat atgcccaggt
     1561 taaacggatg caagttgcct taggtaccgg ggaaataata gattgcaagc tgctttatac
     1621 aaacaaatgc aaaatctgtt atcccctccc ggaaagtgtg gatattcagg aggagaacca
     1681 atgagaaaaa gtgtgggcat tactgttttt ggatgcgaac aggatgaggc aaatgctttc
     1741 cgcgctttat cgccggattt tcatattatc cctacgctga ttagcgatgc gatatcggca
     1801 gacaacgcaa aattggccgc tggcaatcaa tgcgttagcg taagccataa gtccgaggtt
     1861 tccgaggcga caattcttgc gctgagaaag gtcggggtaa aatacatttc tacccgcagc
     1921 atcggctgcg atcacattga tacgactgcc gccgagagaa tgggaatctc ggttggcacg
     1981 gttgcgtatt cgccggacag cgttgcggat tatgctttga tgctgatgct gatggccata
     2041 cggggtgcaa aacccaccat gcacgccgtg gcgcaacaag atttcagatt ggatcgtatc
     2101 cgggggaaag aactggggga tatgactgtg ggagttattg gaaccggcca tatcgggcaa
     2161 gcggtcgtca aaaggctgcg gggatttgga tgccatgtgc tggcctatga taacagccga
     2221 aaaatggatg cagattatgt ccagcttgat gagcttctaa aaaacagcga tattgttacg
     2281 ctccatgtgc cgctttgtgc ggatacccgc catctgatcg gtcagaagca aattggagag
     2341 atgaagcaag gcgcattttt aatcaacacc gggcgcggag cgcttgtcga taccggggcg
     2401 ctggtggagg cgttggaaag cggaaagctg ggtggtgcgg cactggatgt gttggagggc
     2461 gaggatcagt ttgtttatac cgattgctcg cagaaagtgc ttgaccatcc ctttttgtcg
     2521 cagcttttaa ggatgccgaa tgtgatcatc acaccccata cggcgtacta cactgagcgt
     2581 gtgctgcagg atactacaga aaaaacaatc aggaattgtc tcaattttga aaggagttta
     2641 cagcatgaat agaataaaag tcgcaatcat cttcggcggt tgctcggagg aacatgatgt
     2701 gtcggtaaaa gccgcaatag aaattgctgc gaacattgat acggaaaaat tcgatccgca
     2761 ctacatcgga attacaaaaa acggtgtatg gaagctatgc aagaagccat gtacggaatg
     2821 ggaagccgac agtctccccg ccatactctc cccggatagg aaaacgcatg ggctgcttgt
     2881 catgaaagaa agcgaatacg aaacacggcg tattgatgtg gctttcccgg ttttgcatgg
     2941 caaatgcggg gaggatggtg cgatacaggg gctgtttgta ttgtctggta tcccctatgt
     3001 gggctgtgat attcaaagct ccgcagcttg catggacaaa tcactggcct acattcttac
     3061 aaaaaatgcg ggcatcgccg ttcccgaatt tcaaatgatt gataaaggtg acaagccgga
     3121 ggcgggtgcg cttacctacc ctgtctttgt gaagccggca cggtcaggtt cgtcctttgg
     3181 cgtaaccaaa gtaaacggta cggaagaact taacgctgcg atagaagcgg caggacaata
     3241 tgatggaaaa atcttaattg agcaagcgat ttcgggctgt gaggtcgggt gtgcggtcat
     3301 ggggaacgag gatgatttga ttgtcggcga agtggatcaa atccggctga gccacggtat
     3361 cttccgcatc catcaggaaa acgagccgga aaaaggctca gaaaatgcga tgattacagt
     3421 tcccgcagac attccggtcg aggaacgaaa tcgggtgcag gaaacggcaa agaaagtata
     3481 tcgggtgctt ggatgcagag ggcttgcccg tgttgatctt tttttgcagg aggatggcgg
     3541 catcgttcta aatgaggtca ataccctgcc cggttttaca tcgtacagcc gctacccacg
     3601 tatgatggcc gccgcaggaa tcacgcttcc tgcactgatt gacagcctga ttacattggc
     3661 gttaaagagg tgataagtat ggaaaatggt tttttgtttt tagatgaaat gttgcatggc
     3721 gtccgttggg atgccaagta cgccacatgg gataacttca caggaaaacc ggtggatggg
     3781 tatgaggtga atcgcatcat cggcacaaag gccgtggcgt ttgctctgcg cgaggcacaa
     3841 atccatgcgg ctgcgcttgg ctatggcttg cttttatggg atggatatcg gccaagaact
     3901 gcggtggact gcttcctgcg ttgggcagcg caaccggagg acaatctcac aaaagaaaaa
     3961 ttttacccca atatagagcg agccgagttg attacaaagg gttatgtggc ctcacaatcc
     4021 agccatagcc gtggaagcgc aattgatctt acgctctacc acctggatac aggggaactt
     4081 gtttcaatgg gaagtaactt cgattttatg gacgaacggt cgcatcatac agcaaaaggg
     4141 ataggggatg cagaggcaca aaatcgaaga tgcttgcgta aaatcatgga aagcagcgga
     4201 tttcagtctt atcgctttga atggtggcac tataagttga ttgatgagcc ataccccgat
     4261 acctatttta attttgccgt ttcataatga aagtattggt tttgtatcag attttggatt
     4321 tgataaaatc tatgctggtc atggacaatc ttttactgga aagtggttaa agcatctttt
     4381 gtaatacatt gcaaattata gtattcaatc tgcccagcgg cagaaaagaa aaaaaccgct
     4441 gttgggcaga cctttcccac gcccattttg tattttgctg gcggcggttt tgagaaatca
     4501 aggccgccgt tcttttctgg cagcaaaagg tgtggcggcc cataggagga tgccgctatg
     4561 ctgttttgct attgccatgc tctaatccac ttccaccact caaaaaaagc cttgcccctc
     4621 c
//
LOCUS       DAYYNG010000183         4612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000183 DAYYNG010000000
VERSION     DAYYNG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.186"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..807
                     /locus_tag="ACHBFA_002656"
     CDS             46..807
                     /locus_tag="ACHBFA_002656"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9396070.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(922..1362)
                     /gene="fabZ"
                     /locus_tag="ACHBFA_002657"
     CDS             complement(922..1362)
                     /gene="fabZ"
                     /locus_tag="ACHBFA_002657"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9396071.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            1582..2337
                     /gene="fabI"
                     /locus_tag="ACHBFA_002658"
     CDS             1582..2337
                     /gene="fabI"
                     /locus_tag="ACHBFA_002658"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM9396072.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(2447..3499)
                     /locus_tag="ACHBFA_002659"
     CDS             complement(2447..3499)
                     /locus_tag="ACHBFA_002659"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9396073.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            3625..4545
                     /locus_tag="ACHBFA_002660"
     CDS             3625..4545
                     /locus_tag="ACHBFA_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM9396074.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1418 a          905 c          863 g         1426 t
ORIGIN      
        1 gttatcatta aagataacaa aatagtaaat tggaaaggaa cagttatgaa gaaaaataaa
       61 gttattttaa gtattagttt agcagcagca ttgctgtttg ctgctggttg ttctgcaaac
      121 aacacgactt cttctagttc gtctacagaa agttcaaaaa gtacatctac tgcggaaagc
      181 acgactgcca gtagcgtcga tttgaacgca ttggaattac cacaattgac gaaagatgtc
      241 gccgaaaatg aagatctggt cgaaatggtg acgaataaag gcacaatcaa aatcaaatta
      301 tttcccgaac acgcaccaaa agcggttgaa aactttatga cacatgcaaa agacggttac
      361 tacaacggac tgactttcca tcgcgtcatc aaagatttca tgatccaagg tggcgatcct
      421 aaaggcgatg gcactggagg ggaaagcatt tggggagaag gatttgaaac agagatcaac
      481 aaccaccttt ataatataag aggtgcgtta tctatggctc gctcacaaga tcctaacagt
      541 aacggcagcc agttctttat cgttcaaaat acagatgata tgcatgatgg tcttttaaaa
      601 gacgactatc ctcaagcaat catcgacgct tataaaaacg gcggttatcc ttcactagat
      661 ggccattata ctgtcttcgg ccaagtgatt gaaggaatgg atgtcgtgga tgctattgca
      721 aatatcgaaa cagatagttc agacaaacca aaagaagaag taaaaatcga taaaatcaat
      781 atactgcaag aagcaaaaca aaaataaaaa aataacgtct gaatcctgca tagatgacaa
      841 cgtatgctag gattcaggcg tcttcttttt acataaaaag caagtaagct tccttttgtt
      901 ttgctatatc aggttttata gtcaagcatt ttggttgacg atgaacgtga actcgcactc
      961 acagactttt ttatcatcta catatgctgc agataacgct gtaccgactg atcccttcat
     1021 tttggtgatt tcaaaatgac atttcagcac atctcctgga acaacttttt gacggaattt
     1081 ggccttattg atcccaccga tataagctct ttttcctaga aactcttctg attttaggat
     1141 aagtatcgaa cctgcttgtg ccagtgtttc tagaatcaac gcacccggca tcactggatt
     1201 gtcaggaaag tgaccttgga aaaaatcttc attgattgtt acattttttg tcgcaacgat
     1261 cttttttcct ggttctaaag tatctacata atcaatataa caaataggat aacgattagg
     1321 aatcaattcc attacttcag tagcagataa tacgtaagac ataattaaac tccttctttc
     1381 aggcaattgc cattttcatt actttgacta tcaaagtatc atattcctaa atatttagca
     1441 atcttttttt aatttaagaa aaaatagttt gacaatcaaa acattttgac taataattaa
     1501 gaaatcaaga agttatttta gctattattc tctatttttt tacgactatc aaatttttat
     1561 cagaaacaaa ggagagtcat tatgtcattt ctagaaggaa aaaaaatcgt cattatgggc
     1621 gtcgctaaca aaaaaagcat tgcttggggt tgtgctgaag caatgttgaa acaaggggca
     1681 gaaattatct atacttacca aaatgaacga atgaaaaaat cattagtcaa attggtcggc
     1741 gaagaagcat tcactgtaga atgtgatgtt tctagcgacg aaagcatcga aaaagcaatg
     1801 aatgccatcg gtgaatatgc tggggagatc cacggccttg tccatgcagt tgcttacgcc
     1861 aacaaagaag aattgtccgg aaatgtgtct gatatttcca gagacggcta tcaattagct
     1921 caagatatca gcagctactc tttaattgct gtatcaaaat acgcacaacc gtttcttgca
     1981 ccaaacagcg ggatcgttag catgacttat ctaggttcag aacgcgcaat cccgaattat
     2041 aacatgatgg gtattgcgaa agcttctctt gaagctgcta tccgttattt ggcagctgag
     2101 ttctcgccta aaggaattcg ggtcaatgga atctcagctg gggcaatcaa aacattagct
     2161 gtcacaggtg ttaaagacta tcaaaaactg atccagttat ctgaatctcg tacgcctgat
     2221 cagcaaggtg tgacgatcga agaagtcggt aatgcttgtg ctttcttaat cagcccactt
     2281 tccagcggaa tcatcggtga tattatctac gttgacaaag gtgtccatct ttcataaact
     2341 tttgttgcat tctttaaagt aaaacatata aaaatcagga aaaaactttt taaagtctct
     2401 tcctgatttt ttctttttac gtttttttca actcattact ttgaaattat tccacgactt
     2461 caatccagcc ttctggtgct tctatgtctc caaattgaat acctgtcaat tcatcataca
     2521 accgttttgt tactggtcct acttccgttt cactataaaa tacatgaaga ttgttattgt
     2581 gataaatccc accgattgga gaaataacag ccgctgtacc acatgcacca gcttctgcaa
     2641 attgatctag atcatcaatg aaaatatctc cttcaattgc ttgcagtcct agtcgttctt
     2701 cagctaactg caacaaggaa tatttcgtga tacttggtaa gatagagggc gattgcggag
     2761 tgatgaattt tccgtctttt gtaattccaa agaaatttgc tgcacctact tcttcgattt
     2821 tcgtatgtgt ggctggatca agataaacac aatctgagta atccagttct tttgcttctg
     2881 ttcctggaag taaactcgct gcgtaatttc cgccgacttt tgctgcacca gtccccttag
     2941 gagcagcacg atcgtaatca gatacaataa aattcgttgg ttttagtcca cctttgaaat
     3001 acgctcctac tggcgtacag aagacgacaa ataaatattc tggagctgga tggacaccaa
     3061 tgttttcacc gaccccaatc agtaaagggc gcaagtataa tgtagcgcct gttccatagg
     3121 gcggcacaaa atcttcattt gcttttacca cttgcttgac agcatcaacg aacatctttt
     3181 cagggaccat tggcatcagc aatcgtctag cactttgatt catccgtcga ctattttgtt
     3241 caggtcgaaa gagattgacc ttgccgtctt ttcgacgata agcttttaga ccttcaaaac
     3301 attgctgacc ataatgaagc gctggagagc cttcatgaat cgtcaggtaa ttatcttctg
     3361 tcaattctcc ttcttcccat tgtccgtctt tccatttggc aataaaacgc caaggagttt
     3421 tgatataaga aaaacctaga ttgttccaat cgatttgcat atacttacca ccttcgttat
     3481 tttctccaca ttttatcatt atccatatta tttgtcattt tattttcagc ttttttcctt
     3541 cattatagtt atttttgcta tttccttttt tgaaaacttc ttggtacaat acagaaagaa
     3601 tcaaatttta gaaaagagga ttttatgttt tcattattag caactgccgg tacgacagac
     3661 tcttctggcg agacattgac agatcaagct gtcagcaaag taaatgcgtt tcagcggttt
     3721 tgggacggga ttgattggga cgccattttg gctgccttta tccaaaaaag tttatatctg
     3781 atttttttga tcgtcctttt ttggattctt caccgtgtgg gaaaatatct gattgataag
     3841 tcttttaatc agtattctaa aaaagccatt ctaaacgaga gccgcttaaa gactttacat
     3901 agtcttttaa atactatttt tcattatacc cttggtttct tttttatcta tgcaatttta
     3961 tctgctatcg gtgtgccgat cggatcactt ttggctggtg ccggaattgc aggtgtggca
     4021 atcggtcttg gtgcccaagg gttcatgagt gatgttatta ccggattttt tattatcatg
     4081 gaacaacaga tggatgttgg ggactacatc aagctagcaa acttaacgat tgaaggaacc
     4141 gtcgtatctg ttggtatccg tacccttcag ctgaaagcaa cggatggtac tgtccatttc
     4201 atcccgaatc gaaacatcac cacgatcagc aatctctctc gtgcaaatat gcaagtactt
     4261 ttagatattc ggattgttcc tgaagaagga tatgacaaaa tctatgagat cattgatcga
     4321 gtaaatcgta cattagctga aaaataccaa gaagagctcc aaactgaacc ttctatcttt
     4381 ggattagtgg atcttggaaa cagcaatttt gcgattcgta cggtctgcta cgcattgaac
     4441 ggaaaacaat atgccttaaa agaagagttc ttgagctcct atgtcaaaga attgacagct
     4501 tctggtttca cgattccgaa cagtccaatt gctgtcggaa aataatttgg acaaacgatg
     4561 caacgaatct cactaaaaag agcgtgggac aaaagtaaag aacacttttg tc
//
LOCUS       DAYYNG010000184         4592 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000184 DAYYNG010000000
VERSION     DAYYNG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.187"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..1585
                     /locus_tag="ACHBFA_002661"
     CDS             167..1585
                     /locus_tag="ACHBFA_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9396075.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1656..3125
                     /locus_tag="ACHBFA_002662"
     CDS             1656..3125
                     /locus_tag="ACHBFA_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9396076.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3135..3602
                     /locus_tag="ACHBFA_002663"
     CDS             3135..3602
                     /locus_tag="ACHBFA_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9396077.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3638..4510
                     /gene="scrK"
                     /locus_tag="ACHBFA_002664"
     CDS             3638..4510
                     /gene="scrK"
                     /locus_tag="ACHBFA_002664"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9396078.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         1521 a          664 c          954 g         1453 t
ORIGIN      
        1 gaactttcac ttccagtaaa gctcataaaa aaaaatacag cataagttaa atcggcgaaa
       61 aattccttcg tcgatttaaa aatattttta tgaaacgggt tgacatatga aacgggttga
      121 catatattag ttgtgaagat aaaaaccttt tcactggagg aagaaaatgg agaaattatt
      181 aattaaaagc aataatcatc aactttacta ccaacctaag gatgtttggg tcggagatgt
      241 tatgccttac gggaaagaag gaaaattctt tatttatcat caacgtgata ctagaaatcc
      301 agttcctttt ggagaaccgt ttggctgggc tttagcaact acgaaagatt ttgttcattt
      361 tgaagacttt ggggaatctc ttttaagagg ggatgacaat gagagagacc agtttatcta
      421 cgctggttct gtatttgagg caaatggaaa ggttcacgca ttttatacag ggtataatcg
      481 tgaattctta gcgcagggaa agacatctca agtgttgctt catgctgaga gtgatgattt
      541 tgttcattgg gaaaaatcag aaacagcatt ggagttagaa ccacaagaag ggtatgatat
      601 tcgaaactgg agagatccat ttgtcgtttg ggatgatgaa aaagaagaat atttgctaat
      661 tttaggggcg cgaaagggtc aggataaaca tctacaaacc ggacgtttag tggaatttac
      721 atcaaaagat ttaactaatt gggaattcaa aggtgatttt tgggctccgg gattgtatac
      781 catgtttgaa atgccagaac ttttcaagat tggtgactgg tggtatcttg tctattcaga
      841 atactcagat ggaaataaaa tactctatcg aatgagtcaa agtttggaag gtccttggat
      901 taagcctaaa gatgaagctt ttgatggacg ggcttattat gcaggtagaa cagcttatga
      961 tggaaaacgt cgagtcctat ttggatgggt accaacaaaa gatcaagaaa atgataaaaa
     1021 caattattta tggggaggta ctttggttcc tcatgaagtc tatcaaaaag aggatttttc
     1081 acttggggta aaacccatag acgaagtgtg gaatgcattc ataaatttac atgatattgc
     1141 cgatgtagag atagaaacag cagacacaag agaagaagta acgcttgtca aaaatagtgg
     1201 aactttgttt aatttagaaa caacaatttc atttgatgag aatcttagtg acttttcact
     1261 gagattatat aaaaaccttg agacagatga atcttacgaa tatcgcttca atttagatga
     1321 ggggcgtctt acatttgata aaaacccttg ttacccttgg tttcaatata tggataaagg
     1381 acttattaga cctatatctt taaagcctaa ccaatcttat aaactaaaag tgattgttga
     1441 tgacaatata atgacacttt atattgatgg aatcgctctt aacgctcgtg tacatggttg
     1501 ttttggtcaa gcaattacta ttacagcaac aaatggatca atgactgcaa taaataccaa
     1561 gttttcaaat gaaataaaga aataataaac gtcaatcatg tggcaggtct gaaaatcaaa
     1621 catgacctgt cacatttaaa agggaggata aatgaatgga ctacaaaaaa attgccgaag
     1681 atgtattgga aaacgttgga ggaaaagaga atatctcaag cgctacacac tgcattactc
     1741 gtttacgttt aattcttaaa gacaaaagca aagcgaatat ggaaatgctt gaggaaatag
     1801 aaggtgcaaa aggtgttctt ttcaatagtg gacagttaca agtaattttc ggaacaggta
     1861 taattgaaaa ggtttacaaa gagttcgttg aattgacagg agctaaggaa gtatccgttt
     1921 ctgaaatgaa agacgaagga gtcaataatt caggaaatag acttcaacga gcttttaaag
     1981 ttttctcgga tatctttatt ccgatcattc cagcttttat tggggcagca atggttttag
     2041 gaattcgttc gttgattacc actcctggaa tgtttggaat ggatggctca ttcgctgatc
     2101 acaatgtatt cctaaatgat ttatccaaat ttctgggtat tattgcaacc acatttgatt
     2161 atttaccagt tttagttatg tattctgcaa caaaacgttt tggcggaaac cctattttag
     2221 gattacttgt aggatttgtt atgattcatc caggactagc caatcggaat gatttcgtca
     2281 tgggaactat aaagcctgat tattggcatt tatttggatt aagcattcca gcagttgcgt
     2341 ttcaaggggg tgtttttcca gcaattttga cttcttggtt tttagcaaaa gttgagaaaa
     2401 ttacaaataa atattctcct caagtacttg cttacatttt agtcccaact gtgacaattg
     2461 ttcttgccaa ctttgcacta tttttagtgt ttggtccatt ggggaatttt atcggtaatg
     2521 ttttaggtgg aatcattgat ttcctatata tgaagatggg tgcctttggt gcttttgttt
     2581 ttgcaggcgc gttacaacca ttagttgtaa caggaacaca acatgctatt caaggtattg
     2641 aggcaaactt agtcgcaaca actggattca attatatcca acctatttgg tcagtttcaa
     2701 ttattgccca aggtggcggt tgtattggta tgtatttact ctttaagaaa aaatctaaag
     2761 atagagatat tgcgatttca agctttattc caacactggt aggtattact gaaccggcta
     2821 tctttgctgt taatttgaaa tattcaatcg ttccttttgt ttgctcgttt attggtgctg
     2881 gttttggtgg agcatttatg aaaatttttg atgtaaaagc cattggacaa ggattgactg
     2941 tattgccagg tctgactatt gctaatccag ttcttcctta tatatttggt aatctcatag
     3001 cattcatttt accaatcatt tttattctcg cttatgataa atataaagtg tttatccctg
     3061 aagctaaaca ccgtaaattt gcagaaaaaa ctgactcaaa aaaattgcca gttcaagtaa
     3121 agtgagatga gagcatgtta aattttttta gaaaaaatga aataagcgtt gtagcacctg
     3181 caaccggtgt ttttgaaaaa ctggagaaat taagtgatcc agtcttttca aaaggaatga
     3241 tgggacaagg atttgcaatt gatgcaaaaa acgaaacgat tgtatctcca atcgatggtt
     3301 ttgtttcaag tatttttcct acaaaacatg ccttaggaat aaaaacaaaa tcaggaattg
     3361 agatacttgt tcatgtagga atagatacag ttgagctaaa tggtgaggga tttgatatta
     3421 aagttcaaga aggacaaaaa gttaaggctg gggatattct tatgaccatt aattttgtag
     3481 tttttgaaaa gaatcacaag actaaagatg tcattgttgt ttttccagac taccaagacg
     3541 aaattttcat tccgaaactt gaagaagtaa aacaaggaca agaattgttt gttcttggct
     3601 aatgataagt aattaataat agaatcgagg aaaatgtatg gtttttgggg ctattgaagc
     3661 tggtggaaca aaatttgttt gtgccattgg agataaagat ttgaacatat ttgaaaaagt
     3721 cagctttccc acgactacac ccaaagaaac aatgaattta gttttagatt tttttaatca
     3781 tcataaagat gatttagaag gtatcggagt gggatcgttc ggtcctattg atattcattg
     3841 tgaatctaaa acgtatggct tcattacatc aacgccaaaa cttgcttggc aggattttga
     3901 ttttattgga agtttaaaaa agcaacttaa tattccaact gcttggacga ctgatgtaaa
     3961 tgcctcttgc tatggtgaat atgtttctgg aaatggcaga ggcctttcaa gtgttgttta
     4021 ctataccgta ggaacgggca taggaggtgg cgctatccaa aacggaaact tcattgaagg
     4081 ttttagtcat ccagaaatgg gacatatgct tgtaaaacga cacaataaag acaacttttc
     4141 tgggacatgt ccattccacg gagactgttt agagggctta gctgctggtc cggcaatcga
     4201 aagaagatca gggaaaaagg gacaagaagt gactgaaaat gacgttactt gggaaataga
     4261 agcttattac attgctcagt gtgcatataa tacaacattg atgttatcac ctgatgtcat
     4321 tatatttggt ggcggagtaa tgaaacaaaa acacttaaaa aagaaagtac aagataagtt
     4381 tattgagttg atcaacggat atgtcgctat tcccgatgtt gacaattata ttatgacacc
     4441 aaaattgtta gataattctg gtattgtagg ttgtttagct ttagcaagag acgttaaaga
     4501 aaaatcttaa tttggaaaaa agatcgtctc gactatttgt ttggttgatg cgatcttttt
     4561 tttcaattag taattgaagg gacgcctaaa ag
//
LOCUS       DAYYNG010000185         4586 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000185 DAYYNG010000000
VERSION     DAYYNG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.188"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..648)
                     /locus_tag="ACHBFA_002665"
                     /pseudo
     CDS             complement(1..648)
                     /locus_tag="ACHBFA_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570903.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(970..1191)
                     /locus_tag="ACHBFA_002666"
     CDS             complement(970..1191)
                     /locus_tag="ACHBFA_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396079.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1289..1534)
                     /locus_tag="ACHBFA_002667"
     CDS             complement(1289..1534)
                     /locus_tag="ACHBFA_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396080.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1774..2388
                     /locus_tag="ACHBFA_002668"
                     /pseudo
     CDS             1774..2388
                     /locus_tag="ACHBFA_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2758..3759)
                     /locus_tag="ACHBFA_002669"
     CDS             complement(2758..3759)
                     /locus_tag="ACHBFA_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM9396081.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3918..4184)
                     /locus_tag="ACHBFA_002670"
     CDS             complement(3918..4184)
                     /locus_tag="ACHBFA_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9396082.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(4174..4530)
                     /locus_tag="ACHBFA_002671"
     CDS             complement(4174..4530)
                     /locus_tag="ACHBFA_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9396083.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1425 a          843 c          639 g         1679 t
ORIGIN      
        1 agtagacacc gaaaagccga agagcgttcc atttcttcgg ttctttttat atattcctcg
       61 aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg agactttgat aaaatttatt
      121 tcgtcgttta ataggtcgat ggtcttgttc tattaaattg ttaagatact tcacagttcg
      181 gtgctctgtc ttagtatata aacccacact ctgtaacttt ctaaaggcgg agccaagaga
      241 aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac tgtttatgga gtcgttttaa
      301 gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac cagatatcta aggttaagcc
      361 gtccgcatca attgcacgat aaagataatg ccaacgtccc ttaattttga tataggtttc
      421 gtccattttc catgaataga aggattgtct atttttcttc ttccaaagat aatagaggac
      481 tttgctgtac tcttataccc aacgataaat cgtagtatga caaacattta ttccacgatc
      541 atataacaat tcctgaactt cacgatagct tagattgtaa cgcaggtagt aaccaacagc
      601 gacaataatg acgtcttttt tgaattgttt gcctttaaaa tgattcatta ctctgtcctc
      661 tctgtctttt ttctcaattt tacactaaaa tagatttttt ggaaaacttt gcaacagaac
      721 cgtttttact atatacttct tcccagaact tgtccgtctc cttcaaatta tctctacctc
      781 tcgagttcat tgggtggttt gcccatttat taaacatgga atcacctttc tcatactcat
      841 atatttatgt cctatttcaa ttatatttat atatgcttaa ataaatttgt atcctgaaat
      901 ttattgtaat atgaagctgt attttacata tagtaaccaa agagttaata aaataaaaag
      961 agcgaaccat tatagatcag ctcttttttc attgctgttt ttaatcttat ctagaatcac
     1021 ttcaaatgtt tcaaaggtat ttacatctaa ctgacataac caaagcaagt acttcttttc
     1081 tttgtaagtc caatcaatat tacggtaaga ggacattaga ttatggtacc ttgctctttg
     1141 ttcaactgaa gccaattccc agcgtttttc aaatattttt tcttcttcca ttattgatcc
     1201 ctcgcaagac gctacctcaa acttaaataa tgggcagcga aagtctaatt ccttagatct
     1261 aagtatagca aacaaatatg aaaaaaactt agaaggtagt aaaagttctt ccgtcttttg
     1321 ctttgcttct attcttaatg tgtttcgtta tcggtgccct acaaagattt tcattaatta
     1381 cacactcatt tattgttgtg tattttctga catttctctc ttgatcaatt attttaattc
     1441 catgggcatc ctgatagttc ataggagagg agcggctatc agaaatagtt gcacctatct
     1501 cttgaacttc ttttacaact tctggaaaat ccatccatcc tgcttcagct gtcaaaagta
     1561 ataatctata atatttatct ttccttttca atctaagaaa tcgtcacccc attctttttc
     1621 ttctattata aactatttaa cttttagaat tcaaaaaccg tttgtctatt tagagtttac
     1681 cctaaataga caaaaatata acattgaaaa atttagagtt ctaacgtatg attagggtgt
     1741 attataaacg ttatagaaaa gtagggaata aatttgaaaa ttgcttatat tagagtttca
     1801 tccatcgatc aaaatgaaca acgtcaaatt gaagaaatga aaaagtttgg cgctgaaaaa
     1861 atttttatcg aaaaacaatc tggagcgacg attagtcatc ggccgatttt ccaggaagct
     1921 ttagattttg tcagagagca agatattttt attgtagaag ccattgatcg acttggacgc
     1981 aattatgacg agattatcga ttcagtgaat tatctaaaga agaagaatgt gcgacttatt
     2041 atcaccagtt taccgatcat ggctgaagca attggaaatc ctctcctaga taaattcatc
     2101 aaagacttaa tcatttaaat ctcagccatg attgctgaac aagaacgtac tgaatcgaaa
     2161 cgacgccagg cacaaggaat taaaatcgct aaagcaaacg gcgtctataa aggtcgtcct
     2221 aagttataca gtgcagacac caaagatcct caacgtcgcc ttgtctatag gagtatcgtt
     2281 caggatcttg agaatggggt tgctatctca aagattgcaa cagactacaa tgtgactagg
     2341 caaacaattt accgaataaa aaaagaaatc gaccaactaa ttgtatgatt tcttttttga
     2401 atcacgtaat tattaattag ctttgtcatc aatacagaca atcattttag attcatctag
     2461 ctaagttgat tacatttatg ttaaaaagaa tttagttaac ataaatataa ttgatgcata
     2521 aatcgtcctt atttaaaggc atttaacatt gacaacttag tataatgtat cttatgtaaa
     2581 ctaataagat ttgaatttta ttttatacac actcgataat agatcaattt tatggtcccc
     2641 cgcatttacg attgcacaag agaaacataa agtcgaccta ttagcatttc ttttcaattc
     2701 aaacattaaa aaagtttata cctctgctac taaaaaatga aattatctat ttaaaattta
     2761 aaacggtaaa tctgattcat ccaaattgga aaatggatca gcctgtattg gagctttgta
     2821 tttctccata ataacagata tctttttttc attttttaaa aaataccttt taaatttctt
     2881 atagtcctct gaaatcaaaa tatagcgtga tggatactca tcaaaagaag aatcgtattc
     2941 ttcctttaaa attcccgatt tacttaattt ttctagtaca tcaaatactc tatcactcaa
     3001 gtaactcctt aaattgttat cactttccca ttgaactact tttctataag ttgaattatc
     3061 agttgaaaag atagaatttt catccataaa cgcatatatt agcagcaatc tttcatcatt
     3121 gtcaaattct attggatcaa aaatatctaa gttgtcatca aaatctttat cagttccggt
     3181 aacttttttg ttaattactg aaggtgtctt gtaagaaata gaatctaaat caaagcgttt
     3241 tgattctaaa ttattttgtg ctttctcaat taaataattc actttttttg tgagaaacct
     3301 tctgatattg tctttgctat atgttccata aaaaccctta tcttctaaag agtccctgta
     3361 cttcttaact tctatatact cttcagccaa aacttgtgga actggttcat ctaagaaaaa
     3421 aacccctacc tcgcctttgc tatttttata aaagtaatca atttctgcta tagttcccga
     3481 tctataatta tcaactggag aacccaactt atggccaaaa atcgctaata atatatcact
     3541 attttctaca atttgtttat taataacact ttgcccagag ctattattta tataagtagg
     3601 aatcgcattc gtttcccatc gaattggcat caaaattact tttgtattat ctgtattttc
     3661 tatattccat ctatatatgg tttcttctac ttcattcctc tttgctgcta tatcacctgg
     3721 tgatgctata aatactttta ttagatttat ggttttcatt tattgtacct ttctctttat
     3781 gtctctttaa agtaattttt gtataaaatt tattgacttt tacactatag gacaactttt
     3841 atctatttag tacaaaaagg cccactaaac taattgatta gttaggagcc tattacaggt
     3901 atattttcaa ctcgctttta agcaaattct actaccagct ttttgcctaa tgccctagcg
     3961 atatcatcaa gtttatcgaa agatacgtta tcccctctct caattcgagc aatagtagat
     4021 tgagttgtat gtgctttttg tgcaagctcc gcttgagtta ggcctgcttc ctcccgtgca
     4081 tgataaagag caaccgcagt ttcgagtttt cttccttcac ggtcatatgc ttctgcaaaa
     4141 tctttgtctt tttgcttttc ttcaatcaat tttttaatca tcatttgtga accactcctc
     4201 tctaagttct tttgcatgtt taagttcact tattggtgtt ttctccgttt ttttagtaaa
     4261 tccatgggta atgacatacc ttccattgac cacatggaaa tagatagctc tttggatgtt
     4321 tgatcctacc ttacttctta attcgtacaa attagtgtct aattttttca cccatttcaa
     4381 ccgttgagct actagcatcc cattcttttc tgtttcttct attgtacgta aaagcttcgc
     4441 actatctttt tttggtaatg tttccaacca ttctaaaaat tcatcatgac cattaggtct
     4501 tttaaatgtt tcaaaagata ttttttccat acccaattat atcatatatt gattttatat
     4561 tatagtatat aacataaaaa ttcgaa
//
LOCUS       DAYYNG010000186         4565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000186 DAYYNG010000000
VERSION     DAYYNG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.189"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHBFA_002672"
     CDS             complement(<1..1031)
                     /locus_tag="ACHBFA_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9396084.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHBFA_002673"
     CDS             complement(1113..1802)
                     /locus_tag="ACHBFA_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9396085.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBFA_002674"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBFA_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9396086.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBFA_002675"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBFA_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9396087.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHBFA_002676"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHBFA_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9396088.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHBFA_002677"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHBFA_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9396089.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
BASE COUNT         1309 a          999 c          799 g         1458 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatg
//
LOCUS       DAYYNG010000187         4535 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000187 DAYYNG010000000
VERSION     DAYYNG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.190"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..2311)
                     /locus_tag="ACHBFA_002678"
     CDS             complement(125..2311)
                     /locus_tag="ACHBFA_002678"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9396090.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2529..3212
                     /locus_tag="ACHBFA_002679"
     CDS             2529..3212
                     /locus_tag="ACHBFA_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9396091.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3293..4012)
                     /locus_tag="ACHBFA_002680"
     CDS             complement(3293..4012)
                     /locus_tag="ACHBFA_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9396092.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4324..4530)
                     /locus_tag="ACHBFA_002681"
     CDS             complement(4324..4530)
                     /locus_tag="ACHBFA_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396093.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
BASE COUNT         1201 a          889 c          748 g         1697 t
ORIGIN      
        1 aactgtgttc cagaaatagc ttcttcgaaa ataagctgaa atttcacaaa aatttgagaa
       61 gcaattttcg tacattcctt cttctttctt gaaactgacc acttctgtca caccctctct
      121 tctatcactt tccttttttc acacgactcc cggtttcttt gataaatctc gatactttca
      181 attttcttga atttttcatt tgaggtacac atagatacag acgatttctt gctctagtca
      241 tcgcaacata aaatagtctt cgttcttctt ctatcgtcgt cactggttct gcttttattt
      301 cattaaagtc gattactttc ttttctgtcg ctccgttata aggcaaactt ccttcgttac
      361 aaccaatcaa aaagacattt cggtaactaa gacctttact tttgtgaatg ctctggatac
      421 atagtgcatc aagttttcct tgtctgtaag caatcaagcg ggtttttaat tctttagctg
      481 tcgcttgggt ttcttgcgat aaggcttgga gtcttctgaa agtagcatgc tgctttactt
      541 cttctcgtat ccattcttga tcactctggt cgatttcctt agctatacgt ggcagatgaa
      601 gatagctttg aacaagcgaa tagatttctt gatgctggtc tgttttacgc aacgcttcct
      661 caaactctcg gatttctgca gatttcgatt ttaaattggg gcttatcgac aaaatataat
      721 ctgcaacggc tatattttga tatttaggag aaaagtattg atcgcattca ctttttttca
      781 ggtagcagtc tgccatcagc tgccggagca attgctggta ggcctgattt ttcttttgca
      841 gattcagtgc tgtttgccaa tgaacaattt tttgatagaa atgttggaaa gacttgatac
      901 caaagatacc ttcatatgga ctatctgctt ccagtacaaa aggaatattc gttttcagca
      961 gagattggac aagctgctgc gtttgtgaag ctgatcggta taatatgatt gattccttat
     1021 atttcccatt tttttgaata aatgtctgaa tttgtttgat tagccactga ctttctgctt
     1081 tagtatcaga aaacaaatag gcttccataa cacctgcttc tttttgtgct ggaatcatcg
     1141 cctttggtat ccgatcattt ttattatagc tgatcaatct atttgctcct tggacaatag
     1201 ctgcccgaga acgataatta gttaataaga aaagagtttt ggtaggatat tctttttcaa
     1261 agttcttgat gtatgctggc tctgatcctc tgaatccata gatactctga tcatcatctc
     1321 caacaacaat caacttgatc ctgttttcct ttgtcatccg tttcacgatt tcccattgaa
     1381 gtggattagt atcttgaaat tcatcaatga atagataatc atatttttgg ccgatataat
     1441 gctggatctc ctcattctcc aaagcgcttt tcagattttg gagtacatca ttgaactcta
     1501 gcaattgctg gcttcttttt tgttcttgga gttccgtgag caatccatac agtcgttctg
     1561 atatcggctg tcgtgcattc ttatcaatgg aatcatattt ttctttaact aactgaacca
     1621 tttcttctac tgaaaggttc atattctgcc aatagtctaa ccgagaagca atcgtttttt
     1681 ctccatattg atcaaatata taaagattat atttacgtat cagctgttgc aaaacagcat
     1741 tcactttttg tgcagtatcc tttgaaaagt ctccttgtat tccttgcatt gctacttcag
     1801 gaaattttcg acaaatttca ttgtatatcc taaaaaagat actatggaat gttccgatat
     1861 ggacagcggt ttttctctgg ctgaaattat ctactcgttt ttttagttca tttgcagcgt
     1921 tccttgtaaa agtgatcatt agtatgcgtt cttcgtctac ttgtttttgc gtcatcagat
     1981 aaacagcttt tgcacagatt gtttgcgttt tgccgctccc agctcctgct atgattcgaa
     2041 gatgtttctt catatcatat gtgatagctg ctttttgttc ttgattgaag cgattatagg
     2101 aacgtttctc catcatttta aagaaagagc tgtttccttc actttctttt acttgtactt
     2161 cttcctcaat aagtccgggt gcttcgccat aagccgcttc ataggtagca atgatttctt
     2221 cttgtagccg aaagacttgt tgaagctttt tatacttgtt ctcccagcgt ttcaattctt
     2281 tttgtaatcg gagaatttct ttttcattca actgattgat ttccttccca tctttcttct
     2341 ttgaatttgt ttatttcaag ttggttattt aaagtttcgt tgtctatttt ttctttatac
     2401 taacagacct ttctaaaaat gaaaatggga agcgatatga ttttagtttt ctgaaaattt
     2461 ttcctttttt tcaatcgttt atgctaagat aaggaagaca aaaatattca taaaaggtga
     2521 tgaaaaaaat gcagacttcc tttcacgagg ggcgtacaat aggacttttg ctgacattta
     2581 tcggaggagc catggacagt tatacttata tccaatatgg tgcttttgcc tctgctcaaa
     2641 ctggcaatat cattctatcg atcatccaag catttgacgg agaatggcta agtgttggta
     2701 aaaaagtatt gtctaccctt ttctttttct tagggattct tctaacaaag tatctaattg
     2761 attattttag aaaaaaagaa aaacatttct ggcgcttgtt tgttctatat tatgaagcag
     2821 tgattttttt ctttgttagt ttgacacccg tccatgagca tcccgcatta gtaacgattt
     2881 tgattgcttt tactgcatcg atccagtggg tagcttttga taaaatcaac gggcgtgcct
     2941 acactaattt atttacaact ggaaatttaa aaggggtagc aacgaactta tatgattatt
     3001 gtatttctaa agaaaaagca gattttgatc gatttttcca ctatctaagt gtcgttcttg
     3061 cctttatcgc aggagctatt gtccttgttt tctgtcatca tctgtttggc gcccattcga
     3121 ttttgatcgt ttccatttta tttttgatca tggccctcac tcagacattt catttatggc
     3181 aattttatca tacgaatcgg ttcactattt gatctcaatg aatcaaaaaa actaagcaag
     3241 atgctgctca cagagaaata tctgcgagtg agcaatttgc ttagttttta tattatttag
     3301 ttgtaaagat tccctttgct ttcgggttat ttgtagtgta gcggatcgac tgggcatctg
     3361 cctcttctgt cattttcttt gcccgcatct tgatttcttg tacgatttgt tctccacatg
     3421 ccggatcggc tacgaaatca tatttgtccc catcgatcac tattttggga catatatcaa
     3481 actgttcata ccactcttcg tatcgtcgat tgagttcttt atagtaggaa tagagttcag
     3541 gatcttgttc tagctgttca tattcccggc ctcttttttt gattctcgct aacatagttt
     3601 caaaggatac acgaatataa accagtagat ctggtttctt ttgtggcgaa atttcttcta
     3661 actccttcag catcgtttct aaaagacctt catactgctt tacttctact tcagaaacac
     3721 gccctaaatc tgcattcaaa tgaaacagca tactatcttc gtagatggag cggtctagta
     3781 cgttgtcatc atggctgaaa gcatctttga ttgccaaaaa gcgtttgttc aaaaagaaaa
     3841 tctgtaataa aaaagtatac ttttctggat tagaataaaa tagcggcaat acttcattat
     3901 cttccacgtt ctcataaaaa ggttttgttt ctaaatcttg ggcaagcatt tcagttagtg
     3961 tgcttttccc tgcaccgatc gttcctgcaa caatgatcac aattatcggc atccttcctg
     4021 taataaaatc tgttcttcat tttactactt catttataga aataaaagcc ttgactttat
     4081 ctgctcctta aaagctactt ctctaaaagt agccaaaagt aactcctatt catgaaaata
     4141 aaggctgtga caaaagtctt gtcgcagccc ttttttcaaa taaacagtat tctcctgcca
     4201 acacctcggt cacaaggcaa tattcataaa acatgaaaag ctgtttttta acattattcc
     4261 ttgtaactca gagctaaacg ggagactcac aacctcttta tgctctcaac attttatctg
     4321 tttttattct tctattgtac ttgatttgcg tacaactaac tgccagtgca gattatgagc
     4381 ggtatcattg tcttctactg cgcatttcat gacacgtatc atcacactat tttcatagat
     4441 tttttctatt tttcctttta ttggtgattc aaataaatca gatttaaatg catatgtttc
     4501 gccgacttca aacaattgtt cattttccat aagaa
//
LOCUS       DAYYNG010000188         4461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000188 DAYYNG010000000
VERSION     DAYYNG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.191"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACHBFA_002682"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACHBFA_002682"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM9396094.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACHBFA_002683"
     CDS             2298..3221
                     /locus_tag="ACHBFA_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9396095.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACHBFA_002684"
     CDS             3237..3776
                     /locus_tag="ACHBFA_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9396096.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1663 a          666 c          760 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc gtctctatcc attggtagag
      421 gggctacttg gcatagtcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAYYNG010000189         4407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000189 DAYYNG010000000
VERSION     DAYYNG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.192"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..840
                     /locus_tag="ACHBFA_002685"
     CDS             91..840
                     /locus_tag="ACHBFA_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9396097.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            831..1616
                     /locus_tag="ACHBFA_002686"
     CDS             831..1616
                     /locus_tag="ACHBFA_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9396098.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            1585..2301
                     /locus_tag="ACHBFA_002687"
     CDS             1585..2301
                     /locus_tag="ACHBFA_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9396099.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2403..3422)
                     /locus_tag="ACHBFA_002688"
     CDS             complement(2403..3422)
                     /locus_tag="ACHBFA_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM9396100.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(3441..>4407)
                     /locus_tag="ACHBFA_002689"
     CDS             complement(3441..>4407)
                     /locus_tag="ACHBFA_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM9396101.1"
                     /translation="VCRKKGIFSESVGKILSKVVLTVALPALAFRAFMQDINTELLMQ
                     GINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFFGTPIVSAIYGPV
                     GVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFIWLFQGY
                     LPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLGEVSFKK
                     AASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIVIMMATPAATVAA
                     AYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         1442 a          873 c          801 g         1291 t
ORIGIN      
        1 ctccagaaca cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct
       61 gccatattga tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc
      121 tctagcattg ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt
      181 gacccgacac atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat
      241 tttatcgttg tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc
      301 ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc
      361 tctgaaaaag aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat
      421 caagagatta ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc
      481 aaagatggac aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata
      541 ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa
      601 atagcagaac agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta
      661 gccatctctt ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac
      721 caaacagagc agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg
      781 ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa
      841 atgggataga aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag
      901 cagtcgcaac tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga
      961 cgaaacaata tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg
     1021 tcatttctgg tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc
     1081 taaatcaaaa ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa
     1141 ttgccggttt tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt
     1201 tagaaagcga ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc
     1261 aagtgttagg gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa
     1321 acggacaggt ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg
     1381 tctgcgatga cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt
     1441 tttcgacgaa gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac
     1501 gccacggtgg atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag
     1561 aattaccaaa aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca
     1621 atggttgcag aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa
     1681 agtttcagaa atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg
     1741 ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag
     1801 caaggaaatg atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag
     1861 aatgtgatcc gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt
     1921 cagctggcga tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc
     1981 gatcagaaat tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca
     2041 ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt
     2101 ctaaaagaag atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt
     2161 atatccgtca agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc
     2221 aaatatttgg aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa
     2281 aagctcacag attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca
     2341 gaagtaacaa ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt
     2401 tcttatctta attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct
     2461 attatttcac gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta
     2521 tttgaaccta catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata
     2581 gcagggtctg gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt
     2641 gcaaaaacat cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct
     2701 tcattatctt gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga
     2761 gctaaaaatc cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata
     2821 ctgacaatat cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta
     2881 gcagcttctg tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa
     2941 acagcttccg tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt
     3001 tcacgactaa acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta
     3061 tacgcagtat ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt
     3121 ggcgaatacg atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg
     3181 atatgattga ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt
     3241 gcaagattgg tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt
     3301 gctgacacga tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa
     3361 atattcaatt tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc
     3421 atactttctt cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca
     3481 acgatccata caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag
     3541 gctaaaagtg cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt
     3601 gtagccatca tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt
     3661 gccgtcaaaa ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga
     3721 ctgtaatacc atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt
     3781 gcgccgattg ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga
     3841 aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct
     3901 gcacctgtaa ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca
     3961 aaagtagcga taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc
     4021 aagccactca tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa
     4081 attgaggaat acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa
     4141 aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag
     4201 cttaagaaaa aaggttttga aactagaata agaataatat aaataactaa tccccaaatc
     4261 aagacattga ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat
     4321 gccagtgctg gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca
     4381 gaaaaaatcc ccttttttct acaaaca
//
LOCUS       DAYYNG010000190         4380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000190 DAYYNG010000000
VERSION     DAYYNG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.193"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..618)
                     /locus_tag="ACHBFA_002690"
     CDS             complement(133..618)
                     /locus_tag="ACHBFA_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM9396102.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(620..1291)
                     /locus_tag="ACHBFA_002691"
     CDS             complement(620..1291)
                     /locus_tag="ACHBFA_002691"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396103.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1554..2747)
                     /locus_tag="ACHBFA_002692"
     CDS             complement(1554..2747)
                     /locus_tag="ACHBFA_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9396104.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2831..3031)
                     /locus_tag="ACHBFA_002693"
     CDS             complement(2831..3031)
                     /locus_tag="ACHBFA_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9396105.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3455..3688)
                     /locus_tag="ACHBFA_002694"
     CDS             complement(3455..3688)
                     /locus_tag="ACHBFA_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396106.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3695..4126)
                     /locus_tag="ACHBFA_002695"
     CDS             complement(3695..4126)
                     /locus_tag="ACHBFA_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9396107.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1100 a         1077 c          845 g         1358 t
ORIGIN      
        1 atctatagaa ttatgtctgt tcctaattca gtttgatgaa ttaactgata aaaaaatatc
       61 tactatttaa agatacaggg ataagtaagt aaaaaaataa catgtcatta gatagtagac
      121 agttccaact ctttatttac cttttttgta tgctgcactg tcaatccaag ttcctaaatt
      181 gttgtatcca tcatcactta tataatcata ttggccatca ataatttcgt caaaccacaa
      241 atattctcca tctggttttt gcccaattat tttaattact gattgactag tagttgtcat
      301 ctggttaaaa tcttttattt tacctatatt aacggccaaa atagcatttc ctcgcttata
      361 actctcctgg atttcatatt taacatattc tcgatcaaga gtttctttac ctatcagcac
      421 cacagtcact gatgttccaa ttaattgatt atctatccag ttttttatag caatatctcc
      481 attacgttta actttctcaa attcagcctt atctacaaaa ccagcttcct ttgttcctcg
      541 caaagtccag ctgtttctaa ctaccattgc tcttgttaca tctgcataat ggaaactaaa
      601 aaaaaccctt ctcaccatat taatcctcct gaatactatt tattaaactg acaatttgtt
      661 caactttttt atctacttct ccttcagtat ctaatttttc tagcgcttga aatttacttt
      721 ttaaggaatc atcaaaataa tcactgaaat tctcttgaat cttatcaaac aaaaccttac
      781 ttgcaccttt ggtaaatcca atcggaacta ctatattttc attttgaaca gataattcaa
      841 attcttcaat cattccatct gctgtaacta tttcacctga ttcaagtttg tttccaaaaa
      901 caaaaatact aatcccagac tctgcaataa tgttttctcg atatcttctc cactcatgag
      961 gcatatcaag gggaagtggt cttaaagaaa gtacgtcttg tatgttattt tttcttaaat
     1021 ttcttctctg ctttagaact cctgaaacaa tactattacc aattccaagt ccgtaacctg
     1081 atactattct ataaccggat tcagaaagcc tatatgctaa tttctcgaca aattttaatc
     1141 ctttcgattc agatgtaaat ggagtataat caacagcact tcccgaaata aaaatagttt
     1201 ttctcaaata cttttttcta atacttttca aaatatcagt aatctcagaa taatcatcta
     1261 cttcaacaac gtttatcgaa aatctggaca aatcattgat ttgtagtctt ctttttgtca
     1321 gttcataatt atatctctct tgatcaaaaa aatcctcaga atcgacaaaa ttttccttta
     1381 aaattatatg ggattttaga ttatcttttt agaagttctt gaatgtcggt atagcccgtg
     1441 tttccgggct tttccggcat ttttgatatt gttagatact tacaaatgtt cttattacct
     1501 ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa aaccggcctt gtgtcaggct
     1561 gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg cgtgtgcata atagttcagc
     1621 gtcatggtga tattggaatg tcccataatg tactgcaagg ctttggggtt cattcccgca
     1681 ttggctaacc gggtgcagaa tgtatgacgc atagcatggg gcgtcgtcgt tttcggcaac
     1741 gcttcttcat ggctcttgtt gtacttcttc acaagcctgc caaacatccc gccataatct
     1801 accgccgtca tgggatagcc gttctgtttg agaaacagga aattggcata cccgtcaata
     1861 atgaacggct ttgcaccttt gcggtttttt atcactcgct ggaacgcctg atacacttcc
     1921 tcattcatgg ggatttttct gacgccgctt tgggtcttgg gttcgtcaat atagtaaccg
     1981 acgccggtat ttctcaaaag ctggtggctc acgatgatta cccggttttc aaaatccaaa
     2041 tctttgtccg tcagtccgca cagttccgaa atgcgaagcc ctgtccccag cagaataacg
     2101 attgcgtcat aatgcttttg gtaaacctta tcaccttgaa taaaggacag aaaactttct
     2161 tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt cgtccagaac gtcactaagc
     2221 tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta tggcggtgta aaaagccgct
     2281 ttcagggaac gcttgtcgtt attgatggtc ttataagaaa ggcccttttc tttcatccga
     2341 agcgcccatt cctttgcgtc ggacagcttc acactgtcaa tcgggcagct ccccagccga
     2401 tcttgttcca gaatacgcat cagccgttcc cggcccagtt tggtgttgtg cttcacattg
     2461 ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg tcattttctt gccgatggtg
     2521 tctatcccgt cgtcaaggtc tttctggatt tctttctcct tttcccggag tgaaatatcc
     2581 tcccgtttac ctgccggggt cttatccgta ggcactaact tccatgagta aacaaactgc
     2641 ggttttccaa aggtatcaac atatttgtaa gcatatcgtc cgtcttttct ctggctctct
     2701 ccagaccgca aaacgcgatt tttgctgtct cgtctttttt ctgacatctc gtcatgctcc
     2761 tttccatgac ggaaagagcc gcatatatgc ttataaatta ttataacata tctgcggccc
     2821 tttttcacta ttagattgtg tccaaagtgt caattatttt ctcaaactgc ttgcgtttga
     2881 tctggatgcg gttgccattc ataatgaccc agccagaggc cggattttcc tcggccagct
     2941 tacgcaattt gttttctccg atacggaaat atttagacgc ttcctcaatc gtcagcgtgt
     3001 atttttccca aataggcaca tcatggttca taatgcagcc ccctttcaca aattcaaagg
     3061 tttgtgtgat ttaggggcat agacggacgg ctgctggcac agctccacgg gactttcacc
     3121 cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac gcttttaacg ctcggactgt
     3181 ggctataagg gagtatcata gtcctgcctg tacgtcgtcg cccgcaagcc gccacgctca
     3241 cattgcggcg tggtgttcct cgctccgctt ttcccgtagg gaaaagtatc atggcgcacc
     3301 agccgtccgg ctcggcacag acagaatgtg tccgtatgcc ctatgatgcg ccgccgttat
     3361 cttgcgggca tatttatcaa ggtgcgatca ggccaccggg aaaacaccga aacggggaaa
     3421 gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt cgtgacgata gcgtggatga
     3481 gcttgatttc cagacggcgc ttcatggcct cgtccagaca cacatgcgga tagccgtttt
     3541 cgtcatagag cgtccgggta cacagcttgt tcatgtaacc gctgtaatac tgcaaaacgc
     3601 gctctacggc tccggcatcc ccagcgcggg cggcctcgat caccgaaagg ggcaaaagct
     3661 caatgccctt gtagttcggg ttcttcatcc gcgtctaacc tcccttcggc atcaacgcct
     3721 ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg ccgctgaacg gcactccggg
     3781 acattcccac aaggccgccg atctcaccat ctgccatatc cagaacacag tgcaggatta
     3841 aaatactctg ctccatgctg ggcagcgcgg caaaggcttc ggcaacaagc tcattgttga
     3901 tatgtaggtc atagccatgc gacgaaaacg taaagctgtc gctggggtag tgatctactg
     3961 tacagagctt gtccaaatct gcctgtgaca aagagcttag tgacacttca cggtcacggc
     4021 ggcgcttcat ttcagccaag tagcttttgg cctcgttacg cagtaccgtc ttgcaaaaag
     4081 catcgaacat gcaccgcgct tcatagtcgc gggggatagc atccatcttc tcaccccctt
     4141 tctgtggggg acttaggtgg ttctctccct ttccgctaac aagactttga caaggccgcc
     4201 agctacccgc taaatacctc ctttttgaaa atttctcaaa aaattttttt ggggacagcc
     4261 gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa acctaccggg cgagaaaatg
     4321 ctttgctatg ctgctgattt acatggtata gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DAYYNG010000191         4283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000191 DAYYNG010000000
VERSION     DAYYNG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.194"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..1174
                     /gene="truB"
                     /locus_tag="ACHBFA_002696"
     CDS             251..1174
                     /gene="truB"
                     /locus_tag="ACHBFA_002696"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9396108.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1178..2119
                     /gene="ribF"
                     /locus_tag="ACHBFA_002697"
     CDS             1178..2119
                     /gene="ribF"
                     /locus_tag="ACHBFA_002697"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM9396109.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2471..3256
                     /locus_tag="ACHBFA_002698"
     CDS             2471..3256
                     /locus_tag="ACHBFA_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9396110.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3269..4120
                     /locus_tag="ACHBFA_002699"
     CDS             3269..4120
                     /locus_tag="ACHBFA_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9396111.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
BASE COUNT         1444 a          698 c          946 g         1195 t
ORIGIN      
        1 ggaagtgaaa accaaactat ttacctgtaa gccacccata aatccaatca aatggtgcaa
       61 agaaaaaatc ttccattcta ttccctcctt taaaaaagca taacatgatt tctatttacg
      121 tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca ttatgatgtc
      181 aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa aaatgaaatg
      241 gagaaaagac atggacgggt tattaccttt atggaaagaa cgcggaatga caagtcatga
      301 ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg ggggtacgct
      361 tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa aagttatcga
      421 atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt tcagtacaac
      481 gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt ctttatctga
      541 aaaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga taccaccaat
      601 gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca ataatgaaac
      661 agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa gtgaattgac
      721 aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg ggaaaggaac
      781 ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg cgcacatgtc
      841 tgatctgacc cgaacagcaa gtgccggtat gtcccagaag gatgctctga cattagaaga
      901 agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa tagaaaaagg
      961 agtggaacgg ttcaaacgag tagatattac tgatgaagta tggcagaaag taaaaaacgg
     1021 tatgagactt gattatactg tatttgcgct cccagaaatg ccgtcagaag aaattgcttt
     1081 gttttataaa ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa aaaacaaatt
     1141 aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca agatcagaca
     1201 tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg gatttttcga
     1261 cggcgtgcat atcggacacc agaaagtcat aaagacaggt atggaaattg cacaaaaaga
     1321 aggattgaaa ttagcattga tgactttcaa tcagcatcca tctattgtct ttaaaaagat
     1381 cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa aaatggccgc
     1441 tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc atttagcacc
     1501 gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg tttccggttt
     1561 tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg aatatgccaa
     1621 aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa aaatcagctc
     1681 tacccgtatt cgtggattac tagacagcgg acaagtaaaa gaagcagcaa aactattagg
     1741 ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca gactactagg
     1801 attccctaca gcaaatgtca aagtaaagag cacggttcat ttgcctaaag aaggtgttta
     1861 tgtgtcggaa ataaaaattg gggaaacatg gtatccagcc atgggttcaa ttggacataa
     1921 tgatacattc ggtgaaggac gggagctgac tgtagagttg tatattttgg actttaatca
     1981 agatatctat ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag atcaagtgaa
     2041 attttctagt gtagaggaat tgattgaaca actcaaggag gatgaacaag caacagctga
     2101 atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta taactagcaa
     2161 ttacaataaa atgggtgata aataagccca ggaaaggggc tgtgacaaaa gtcttgtcac
     2221 agcctttttt tccaaataaa tggtttttca gaagtagctt cttcggaaat aagccgaaat
     2281 ttcttgaagc tgaccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2341 caattcatga aaacgattgc tagagcgtat catttgcttt agactatata tcttggtgta
     2401 cactcacttt aaggcggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2461 agtgggtact atgtacaagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2521 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2581 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2641 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2701 tacggcttta gaaaattttg ggacactgga cgtttgggtt aataatgctg ggatggaaaa
     2761 tcagcatcca acccatgaac ttagccttga agattgggaa agagtgttaa aagtcaattt
     2821 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga ataataaaaa
     2881 aggaaatatc atcaatatgt cttctgttca tgaacagatc ccatggccaa cgtttgctca
     2941 ttacgctgct tcaaaaggcg gggtaaaatt atttaccgaa acggtagcaa tggagtatgc
     3001 accgaaggga atcagaatca atagtatagg ccctggtgct atccgtacac ctattaatgc
     3061 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3121 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3181 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3241 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3301 tggctggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3361 attaggaact ggtattggag cggtgcttgt cggcttactc gttcaattat tcgcttctcc
     3421 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tttgggttat
     3481 tggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3541 ttcgactgca ttacagttag tcggaacctc gttgattggt gtcttcgctt ttggagaatg
     3601 gtctggaagt atggggaaaa tcattggagc agcagcaatc attcttcttg tatttggttc
     3661 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaattag
     3721 tctattagca tcaacaagta ttggttattg ggtatacagt gcattgccta aactagttga
     3781 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3841 ttatgtgatt ttccgtaaac caaccgcatt ttctgaagac aagagttgga aagcaacgat
     3901 cgttggagcc atctttagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     3961 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4021 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4081 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaattt
     4141 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4201 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagggaaa
     4261 tatctgtctg gatcaataat ttt
//
LOCUS       DAYYNG010000192         4282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000192 DAYYNG010000000
VERSION     DAYYNG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.195"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHBFA_002700"
     CDS             complement(37..1257)
                     /locus_tag="ACHBFA_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9396112.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHBFA_002701"
     CDS             complement(1277..1888)
                     /locus_tag="ACHBFA_002701"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9396113.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHBFA_002702"
     CDS             complement(1931..2863)
                     /locus_tag="ACHBFA_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9396114.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACHBFA_002703"
     CDS             complement(3284..4144)
                     /locus_tag="ACHBFA_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9396115.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAYYNG010000193         4259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000193 DAYYNG010000000
VERSION     DAYYNG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.196"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(605..778)
                     /locus_tag="ACHBFA_002704"
     CDS             complement(605..778)
                     /locus_tag="ACHBFA_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396116.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(781..1179)
                     /locus_tag="ACHBFA_002705"
     CDS             complement(781..1179)
                     /locus_tag="ACHBFA_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396117.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1395..2450
                     /locus_tag="ACHBFA_002706"
     CDS             1395..2450
                     /locus_tag="ACHBFA_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396118.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2470..2823
                     /locus_tag="ACHBFA_002707"
     CDS             2470..2823
                     /locus_tag="ACHBFA_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396119.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3005..3292)
                     /locus_tag="ACHBFA_002708"
     CDS             complement(3005..3292)
                     /locus_tag="ACHBFA_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396120.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3305..4123)
                     /locus_tag="ACHBFA_002709"
     CDS             complement(3305..4123)
                     /locus_tag="ACHBFA_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9396121.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
BASE COUNT         1472 a          734 c          594 g         1459 t
ORIGIN      
        1 tttaatcacc cataaagtat gcgctttcat ttttttggat gagattaata tcaataaata
       61 acgatgtaaa aatcatttat tatgctcttt tataaggata ttaattgatc taatttgaaa
      121 aaaaatcaaa atttaaatgt tttaatatta caaataaaat catgttattg taaaatggta
      181 taaaaaagct aaatatttta tttttgttat tttattagca ggaaaattta aatagtttta
      241 caagaatccg tagaaatata ttggttttgc cttcagatta atactggatc atattaatct
      301 gaatataata tatgaactaa taaatagctt aacctcgcta ttttgttttt tctgatcact
      361 atctatacag cattagtaaa tagaatttta aaaataaaaa aattaagaca aaaaagtatc
      421 aacataaaca tatacatata cacgtttatg ttgattcact taatttgtcc tttaaaaagc
      481 acttatttta atataatgtt attttataat cttattacaa gctgattgtt aggcgttgtc
      541 ctttcattac atttattaac ggttatttta ttgtttaatt tattaattat ttttgatcta
      601 aaacttagct taacaaataa ataattaacc aaccgccaga taaaaatgcg atcattgcta
      661 atacataagc aaagataaaa atatgttcga tcagcttaaa ccctgatgcg attaagcaga
      721 ccaagtaaaa tagaaattct cttacagata atggtaattt tctaactagc atactcataa
      781 ttaacacccc aaagtaatat tgtattttga acattttaca tccaatacag catcaatcaa
      841 ccattgccaa tcaaaaatac aaggcttcat tgaagtatgt ttttcagatg aaaagatata
      901 atataatttc ttcggaataa tattttcagt tgttgggatc aagctaaatc taataccgtc
      961 taactcatag ctaccagctt tctctccatt aaaaatttcc ttagcacttt cttctaaaga
     1021 atcaaaatat ttttcgacag caatctttga agcttttact attgaagcat ttcccttttc
     1081 gataaaatct gcttttgttt taccgacaat aagtaattct tgatcagtaa aatcttctaa
     1141 aataatggta gtgtaattct tatttcttct caatcgcatc aggatcactc cttaatataa
     1201 tttatgtttt tataatatca taataagata aatttgcaag catttattta caccttttta
     1261 tttacacttt attataatta attagacttt tatgattgca tttttttctt ataaacagtt
     1321 gcaactaata gtaaataggt gtaaactatt ttcgtaccga aataccataa acacaaacag
     1381 aaagaggtca taccatgaaa gaaccattat tgttttccgt tgacttaggg aataaatcag
     1441 taaaagaaat gagtacacac gtttctccac tctcacttcc ctctcgtatt ttgaacaaaa
     1501 atatcattaa ttcaggacgt ttttctaata taggttcctc attaaaacga tcactaaacg
     1561 tttctgaata tagcctagta aacactactg aaagctatta ctttgggaag gatatacacg
     1621 agctaggaaa agatgactac gtgatagaat cactaggttt tgggatcagc agatacaagc
     1681 aggaaaccta ccaaaatatc ctatctttct ctttagctaa cctagcacgt ttagaagatc
     1741 aatctgatga cgttttaacc gctaatctag ttttaggtct gccttcatcc gactataccg
     1801 aaacaatcgt caaatacgtg atgcaacaag ccacacgaca acattcagtt gaagttgacg
     1861 gtaaagctta taatataaac gtagataccg tttgggtact gccacagccg attggaacac
     1921 tatataacct cctacttgct gatgatggta gtgtacaaga tgaaacattg atcagtcaga
     1981 agatcggttt tgttgatatt ggcggtggca cgaaactttt tgatgttgtc caaaattttg
     2041 aaattgacga aaacaatcgt gaacaaaagg ataccggcat ttttacttta tataatcgta
     2101 tcgtaaatat gcttgacctt ccgatcagtc ctaatgcttt ccaacttgaa aagattatcc
     2161 gagaaggcaa agaaaaaggc gtatatctat ttaagccaag cggaaatgaa agttttgata
     2221 tttcagaagt agttgaaaga gaaataaaaa catacaccaa tatggttgtt gcagaagttt
     2281 tagctacatt caaaaacttc acaaccttag ataaagttgt ctttactggt ggtggagctt
     2341 ctattatcga caaacaactt gtaaaagata aaattccgca taccttgttt gttgaagaca
     2401 gtgaattttc taatgtaaaa ggcttttaca agtttggttt aactcaataa aacagcgaag
     2461 gagatcaaca tgaataaggg aaaatcagag cgttatgtag ttataatcaa tagagaaaag
     2521 gttagcgagt tacctttagc agagtttctt gaatccgaat atgaaacact aggcgttaaa
     2581 tactctcaga cgatcaaaaa agctttgaac ctatatcggc gtgtaaagaa caatgaagtt
     2641 acaattattg aagcaggcaa cgatagaaac gatagcaaag tagcagaagt aaagaataaa
     2701 ccaactgatc ataaccctat tcaacaagag catccttcaa cggttacatc cgatcattca
     2761 gcaaagaaag aaaagccaat gctgaaaggg ttcaatgcac caagtaacaa agacttactt
     2821 taaaaataaa aaggcgaggt tacaatacct agccttttta ttatgtacaa atataaacat
     2881 aaacatatac gtatacatgt atataaacat gtatacgtat atatccataa acacgtatat
     2941 taaatttagt taagctatac ctaaccgctt attccaataa aaaaagtgat cagctttaaa
     3001 ctgatcactc accaaaaact tcatcataca agttatctat catttcacca agtaaagctg
     3061 aatctgatcg aattcctaac ttttcgctga ttttagatag tttctttctt tgcgatggtt
     3121 gaagcgtgaa ttgataattt ttcttctttt cagaactatc actttttagt ttagctacca
     3181 aagaacttgt tgtttcttta ggtgtttgtt taggttcttc aactgcttca accacttcag
     3241 gaacttcttc tacttcagct tcagcgaatt ttttaaatct attttctttc acttcaatca
     3301 ctccttattc ttgtgccgta tcaattattt tgttgtatag cgtaaacgtg ctttcaaaga
     3361 aatccttatt cttacgatag atattcttat ccttagcgaa ctcagcaata ttcttacctt
     3421 ctaagctagt tgcattaagc aattccttct cttgtacata tccaagataa ttagataacg
     3481 ttggtaccag ttctaggaac tcacgacttg aattagtatt atgtttgatc ctgctaccga
     3541 tcgtagcgta tttagtttcc atcagtgttt caccagtctt aatatcaatc aactgttttg
     3601 ctagtgcatc aatatcttta ccaagcttca gcaaagcttc aaaaccatct ttagatggat
     3661 cagaaatgcc tacgacaaga tcagatacag cccaagcatt ttgagctagt agacctgcat
     3721 cattatgagt atcaatcaga attagatcat acttctcttt aagctgttca tagttaacaa
     3781 ccatccatct aaacagtagg gttctgttgt taggcatatc acgcatttgc atctcaactt
     3841 gttttaaatc ttcacctgct actaaaatat ccatgttctc atttacagct aaaggttcag
     3901 ctgatccatg aagatataaa tgattgattg tattctctct tttcgcttta ctgaaagcat
     3961 caggaaatcg ttttgatagg ttttgcgact tatcttcatc aatatacagc accttcatac
     4021 ctaaattttc gacagcatat ccacctagat tgtagattgt tgttgtttta cctgttccac
     4081 ctttttcaat gtttccagta atgattgaca acttcttttc catgattaaa cctcccttat
     4141 taaccttttg taactccatt atacactata aacacgttta tgtatatgtt tatatacata
     4201 aacgtgttta tatttatatt tgcttttata ttctacaaca taaacatata tatgtatat
//
LOCUS       DAYYNG010000194         4196 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000194 DAYYNG010000000
VERSION     DAYYNG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.197"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1022)
                     /locus_tag="ACHBFA_002710"
     CDS             complement(45..1022)
                     /locus_tag="ACHBFA_002710"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9396122.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACHBFA_002711"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACHBFA_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM9396123.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            2296..2553
                     /locus_tag="ACHBFA_002712"
     CDS             2296..2553
                     /locus_tag="ACHBFA_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396124.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            complement(2595..4019)
                     /locus_tag="ACHBFA_002713"
     CDS             complement(2595..4019)
                     /locus_tag="ACHBFA_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9396125.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         1159 a          890 c          731 g         1416 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa tcttatcaca gtcccttatt
     4141 ctgaataatg atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaa
//
LOCUS       DAYYNG010000195         4144 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000195 DAYYNG010000000
VERSION     DAYYNG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.198"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..164)
                     /locus_tag="ACHBFA_002714"
     CDS             complement(<1..164)
                     /locus_tag="ACHBFA_002714"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9396126.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINAL"
     gene            complement(499..729)
                     /locus_tag="ACHBFA_002715"
     CDS             complement(499..729)
                     /locus_tag="ACHBFA_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9396127.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(744..1694)
                     /locus_tag="ACHBFA_002716"
     CDS             complement(744..1694)
                     /locus_tag="ACHBFA_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM9396128.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1691..2533)
                     /locus_tag="ACHBFA_002717"
     CDS             complement(1691..2533)
                     /locus_tag="ACHBFA_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9396129.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            2854..4041
                     /locus_tag="ACHBFA_002718"
     CDS             2854..4041
                     /locus_tag="ACHBFA_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9396130.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         1322 a          774 c          634 g         1414 t
ORIGIN      
        1 ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa
       61 tgtttataaa aagcacccgt agttacgcct gcggtcaagc acagttgcct tatcttagtt
      121 tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaattt ttggtgagtt
      181 gtaatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt aactatatta
      241 tcttacacag gacttgaagt ttcaatcgtt aacgtctatt actgaactca ttccttagag
      301 ctaaactaat gagaaagagc aaaataactg atacaaaaag caatcctaca agaattaata
      361 gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata
      421 tccagctatt aggataagtc ctatcataat cactccaata gccattttct tatcctccct
      481 aaaaataaag tcttttactt aaaacctttt tttgtaatcg tcaataaatt ttttcccttt
      541 atcagttaat tgaaatccta tatctaaata gtcctcatat ctaagtttag ccagcatctc
      601 acttacagat gctggactaa cacttagaac ttttgctaaa tcattatttt ttaatttctt
      661 ttttcttttt gataattcct gaattgctag taaataaatt agtatagtag tcgttttaat
      721 ttgtaccata cctaccctcc aaattatttc gaaaggcctt cagcaatttt atccaggttc
      781 catttcatca tcgcatagta actatcacca tcttgtcctt tttctgcaat tgaatcagta
      841 aaaatcgttg aatagatagg aatattggta tcttttgata ctgttttcat tggtctatca
      901 tccacactgc tttctacgaa taaggaggga acttttgttg tgcgtaattt ttcaactaag
      961 tgttttattt gatctggtgt tccttcttct tcagtgttga tttcccaaat gtaagcagaa
     1021 ggcacattat acgctttcga gaaatattta aagcatcctt cacttgtaac aatcattttt
     1081 ttatcattcg gaattgaagc aaatttagat ttagcttctt tgtctagaga atccaatttt
     1141 tcaatatact tatctagatt ttctttatag aattttttat tatcaggatc tttttccgct
     1201 aactgttttt caatattttt agcgtaaata ataccgtttt ctaaatttaa ccaagcatga
     1261 ggatcttcct tccctttctc actctgaccc tctaagtaaa taacatctat gccatcactt
     1321 gctgcaaaat agtctttgtt ttcctctttg ttcgcatttt taactaattt tgtaaaccaa
     1381 gcatttcctc cagtttccaa gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt
     1441 ttttgaacat cttcaggcaa aggttcatat tcgtggggat ctttcccaat aggtacgata
     1501 ctgtgtaaat caattttatc ttttgctata ttttcggtaa tatctgctaa aatcgaatta
     1561 gtaactacta cttttaattt ttcgttcgaa gccactttat ctttgtcttt actatttatt
     1621 gaacatgccc cgaatataaa aagagcaatc aaaatcccca gacttcccag taccgctttc
     1681 cattttctca tcataacgat ctctccttct ttcttaaaaa tttttgtttt ggtgagatta
     1741 gaaaactaat tacaaataag aatgccgctg ttaaaacaat acttgatcct gctgctatgt
     1801 taaaactata accaataaac aatccaagca atgatgaact tgctccgact gttgcagaaa
     1861 taaatatcat agttttcaag ctatttgcat ataaatatgc tgtagctgct ggtgtaatta
     1921 acattgcaac aattaatata gttcccacac tttgcatagc tgttactgct actaaagtta
     1981 atagaaccat caatagataa tgataaaaac tgacattcat ccctattgct tttgccatta
     2041 atggatcaaa agaagtgatt aataattctt taaaaaatag aactactata accaacacta
     2101 aaaccgaaac gccaattgta atccacttat caatatcttg tacagctaaa atatttccaa
     2161 ataaaatatg aaataaatcc gttgagctat ttgcaactcc tataaggata actcctagtg
     2221 ctaaaaaaga actgaaagta ataccaattg ctgtatctcc ttttattaca ctattgcttt
     2281 ttataaaagt aataatgata gaagctaata gaccaaatac aatcgcacca ataaaaaaat
     2341 taatacctaa aatataagaa attgctactc ctggcaaaac agcatgagaa atagcatcac
     2401 ccataagaga cattcctctt aaaataataa aacaacctac tgttccagaa acaatcccta
     2461 taactacaga tgtaattaga gcattctgta aaaagtgaaa gtcatacaga ccattaataa
     2521 aattttgaag catattattc cccttgtata ttagagttag ggttttaacc ttccggtttt
     2581 gattggataa ccctatgaac catcgttatt ttaatttggt attaactcat ttcttttgag
     2641 ataatatatt tattggattg agaggctaag ccgttccccc cttgggtcat tgaacccgca
     2701 ctcgagagac tggctcctct ctctgataag tggattgcgt atgagttaga tgttgaaagt
     2761 ccttacttta ctccgtatct cgtacaaggt agtgacgctt ttcagttgag tggatttcaa
     2821 tccaaaattc tttttttgaa aggtcagata tacatgtact ttttaggcat tgatatcggt
     2881 aaacgcactc acgttgcttc tatcatgaac gaggaaggaa aggtattact aaaaggtttt
     2941 tcatttccta acaccactga aggagcagag tcattaattg agcgaatggt cgactattcc
     3001 ggagcaccca gtgactttgt tatagggatg gaagccacgg ggcactattg gctgtccatc
     3061 ttttcatacc ttcacgaatc agattatttg attcacgttg tgaatcccct tcaaacagac
     3121 ggatggcgta aaggtacaga aatacggaaa cgaaaaaacg atattattga ttctgttttg
     3181 attgctgact taatgcgcta tggctcattt gtcgaaacgg tcttatccga tgaaaatgtt
     3241 ttttctttga aacaactttc tcgttaccga acttatttag taggtacagc gagcgacttc
     3301 aaaagaaaaa ttatagccgt attggatcaa gtctttccag aatacgcgac gatttttacc
     3361 aagcagggcg ttttcggaaa agcttctaaa gagttattgc tggcgtttag tgccccgatc
     3421 gacttagaaa acatttcctc agaaactttg gcccaatact tagctgagtt aagcaggaac
     3481 cgaatcaagg cagaaaaagc ggaccaattg aagcaagcag cttctaactc ctttggagta
     3541 aaatttgctc aagaagcgtt caccttccaa ctacgttcaa tgattgaaca attaaaattc
     3601 attgaaagcc aaataaagga aacggaaaga gaaatcaagc acataatgga cactttaaat
     3661 tctgttattc tcacaattcc tggaattggt cctataaacg gtgcgactat tcttggtgag
     3721 atcggagata ttcaaaaatt ttctaacccg aaaaaactcg ttgcttacgc tgggatagac
     3781 gcttccgtga cacaatcagg gcaatatgag gcaacgcata atgttatgag taaacgaggg
     3841 tctccatatt tgcgaaaagc cttgttttcg gcggctttag tagcttctca atgtgatcct
     3901 gtttttagtg cgttttacca gaaaaaagtt tccgaaggaa aacatcatct aactgcctta
     3961 ggtgcggttt ctcgtaagct ttgttacgtg attcatgcta ttttgactaa aaacgagcct
     4021 tatataattg tttctgatta gctattgaac attcaaaaaa actaatggta agaagcttat
     4081 ttgttgtacc cttttttcgg aattatctgt tataaaatat tttttgattt ggctaatttt
     4141 tact
//
LOCUS       DAYYNG010000196         4064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000196 DAYYNG010000000
VERSION     DAYYNG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.199"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1130
                     /gene="bsh"
                     /locus_tag="ACHBFA_002719"
     CDS             156..1130
                     /gene="bsh"
                     /locus_tag="ACHBFA_002719"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9396131.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            1455..3350
                     /gene="asnB"
                     /locus_tag="ACHBFA_002720"
     CDS             1455..3350
                     /gene="asnB"
                     /locus_tag="ACHBFA_002720"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM9396132.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3545..3991)
                     /locus_tag="ACHBFA_002721"
     CDS             complement(3545..3991)
                     /locus_tag="ACHBFA_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9396133.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT         1359 a          662 c          758 g         1285 t
ORIGIN      
        1 ttgttgtact tttaacctac aatatttttt gattccccct gagtactatt ttttataaat
       61 gctagtaaat caatcttctt tggccttttt ttgataaaaa aaatactaga tgtaagataa
      121 aagtgtaaaa acgatttgtt ttaggacggg aaacgatgtg taccgcaatc acttatgcaa
      181 cgaaagacca ttattttgga aggaattttg attatgagat gtcttataat gaagtggtca
      241 ctattactcc aagaaattat cgtttcgatt ttagaaaggt aaaaaattta gataaacatt
      301 atgcaatgat aggaatagca gctggggtat ccaactatcc actctattat gaggctacaa
      361 atgaaaaagg gttgagtatg gcaggactga attttcctgg caatgctgat tataaagaat
      421 tacaagaagg aaaagataac gttgcaccat ttgagtttat tccatggata ctagggcaat
      481 gctcaaccat agacgaagct aaagaattat tggctactat caatctagtc aatatcgatt
      541 ttagtgaaaa actacccttg tcacctttac actggttatt agctgataaa gaaaaatcga
      601 tagtaattga aagtatgaaa gatggacttc atttatatga taatcctgtc ggtgtgttga
      661 caaataatcc gccatttgat tatcaattat ttaatctgaa caattatcgc tcgctatcaa
      721 atggaacacc agaaaatcat ttttcaaacc agatcagctt gaatgtttat agtcgtggga
      781 tgggaggatt gggcttacct ggagatctct cttctgtttc tcgatttgta aaagccacat
      841 ttactaaaat gaatgctgca tcaggtgatt cagaatctga aagtatcagt caattttttc
      901 atatactagg ctcagtcgag caacaaaaag gagtatgcga tactggagaa agaaaatacg
      961 aatatacgat ttattcttct tgctgtaacg ttgataaagg gatttattat tatcgtacgt
     1021 atgaagacag tcaaattact gctgtaaata tgaataaaga agacttggat cgtcatgaat
     1081 tggtcagtta tccagtcata aaagagcaac agataaacta tgtcaattaa ttaatgaata
     1141 gatccgtttg gtatcatatt ttctgataca gtcataatta gacacgatct ttaggtcgtg
     1201 tctttttttg ctggtttttt tggaaatagc atccactaat atttttttaa cacgaagaga
     1261 ataaggtagc ctcaaaaaag aaaacatggt aaattagata ttgggacttt attttctgta
     1321 aaaacagaaa ctttcatctt ttgttttcac gtgacgaaat tgtaagagag aaaatattcg
     1381 tccgctattt attctatgat aagatgcata tgaatgaaat aaacagtaat tttatattag
     1441 gatgtgaaag ttaaatgtgc ggtattgtag gttttgttaa cgacaaggac aacaaaaaaa
     1501 caattattaa tacaatgatg gatcgaatcg ttcaccgtgg acctaatagt tcaggagaat
     1561 atatcgataa acatgttgct ttaggattca gaagattaag tattattgac ttagagggcg
     1621 gtactcagcc tatttacaat gaagatcgga caaagatcat tatttttaat ggcgaaatct
     1681 ataattacca gcctttgaga gaagaattga ttgcagctgg ccacgttttt caaacacatg
     1741 cagatacaga agtccttttg catggttacg aagaatgggg aacagaatta ttgcaaaaga
     1801 tccgcggaat gtttgctttt gctatttggg ataatgaaaa gaatgagtta ttcggtgcaa
     1861 gagaccactt tgggatcaaa ccatattatt atgcagaaat gaacggaaca tttatgtttg
     1921 gttcagaaat caaaagtttt ctgcctcatc ctgattttaa caaagaattg aacaaagaag
     1981 cgttgaaacc ttatatgact ttccaatatt ctccattaaa cggcgaaaca ttcttcaaag
     2041 atgtctatcg tttgcctgaa ggccattatt atacgtataa agatggaaaa atagacattc
     2101 agcaatattg ggatgcagat tttgaaacaa aagaaacgca ttcccgacaa gaatggatcg
     2161 aaaaaatcga cgaaaccgtt caagcttcta ttgaagcaca tacggtcagt gatgtagaag
     2221 ttggttcttt cttatccagc ggggttgatt ccagttatgt aacctctgta ttgaaaccag
     2281 atcattcttt ctcaatcggg tttgatgata aaacctataa tgaagcgatt gaagccagaa
     2341 aactaaccga actattggac ttagataata cagcagcagt gatcgacgga gacatgtcgt
     2401 tcaaagcatt ccctttgatc cagtatcact tagatgaacc tgattcaaat ccttcttgcg
     2461 tacctttgta ctttttagca aatcttgctt cacaaagtgt gcgggtagtc caatctggag
     2521 aaggggcgga tgaactgttt gcgggatacc aaacgtatgg attccatacg aattcaaaat
     2581 tcatccgtgt gattgctcaa ggattgaaaa aattaccaaa aggaacacgc tataatttag
     2641 gacgtaaaat cggaaaaatg aaaaatttcc atggacggat ccatttatat gaatcacttg
     2701 ctcctgcaaa agaatatttt atcggtcatg ctcgagtgtt cgaagaatca gaagcatcag
     2761 aagtgttaac accaaaatat caaacagcac catctgtgga tgaaatcatg actgttcatt
     2821 acgagaaaac agaaggaatc aaagatgaag tcaataagat gcaatatgtc gatcttcatc
     2881 aatggatgcc aaaagatatc ttgttaaagg cagataaact ttctatggca agttctttgg
     2941 aagtccgagt acctttattg gatatcgaag tcatgaaatt ggctcaacaa ataccaagta
     3001 agtttttatt gaatcaaaac aacacaaaag atattttcag acaagctgcg aacaaacatc
     3061 ttccggaaaa atggtcgaat cgtgtgaaat taggattccc tgtgccaatc aaagcttggt
     3121 tgaaagaaga acacggctac gaacaagtca aagcattgtt cgaagcagat tttgcaaaag
     3181 aattttttga tcaagaaaaa atcatgaagt tgctagatga ccatcacgaa ggacgtaaag
     3241 aagagcaaag aaaaatctgg acaatcttca gtttcttgac atggtataaa gtctactttg
     3301 tagatgaaac gattccgcaa gcagaagcaa tcgactatgt gactgtttga tgaaaatcaa
     3361 atatcaataa aaaagaggtt ttctcctacc gtttaacact gagtgataag aaacaatttc
     3421 aaaaaacagc tcttcccgtt ttatagatat tatgacttat ggttcggagt tggcagagga
     3481 acaccattgt ttcgaacata agggggttgt gacacgattt tcgtcacaac ccctttacat
     3541 atgttcaata atcgatttct ttttcttttg tcccatcttt tttataaatg aacaaagttg
     3601 attctttgtt ttgtgctact tgctttcctt tttcgattgc ttcttttttt gtatcaaaat
     3661 ggttgcttgc cttttttgcc cctttagatt tcacgatcca ttgttcttcc tcatattcta
     3721 cgatcacatc tgaatcaagc agttttccag cacgaggatt cgtatcgtgt tcatccgtct
     3781 tgcttggatt tttttctttt tcgaaagttc gtttttctga ttcactagca tcttcatacc
     3841 atttttttgc ttgatcaatg gcaattggga tcgcacggtt atcagggtac ccttcatcta
     3901 ataatgcatt tgcgatgtcg attgcttttt ttcttgtcaa tttatctaag tttttcattg
     3961 atgcaggaaa gtccttcatg ttccatggca tattagtatc cccctttatt aaaaaacata
     4021 atctatataa acaagtgtaa gggtatttat acaaaaagaa aaag
//
LOCUS       DAYYNG010000197         4041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000197 DAYYNG010000000
VERSION     DAYYNG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.200"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(606..1487)
                     /locus_tag="ACHBFA_002722"
     CDS             complement(606..1487)
                     /locus_tag="ACHBFA_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9396134.1"
                     /translation="MLYGAIEAGGTKFVCAVGTKDLEVIERIQIPTTTPIETMNNVID
                     FFKKHQEQLAAIGIGSFGPIDIHPNSATYGYITSTPKKAWQNFDFIGYLNKNLKELPI
                     VFTTDVNAAAYGEYVAGNGKGRSTIVYYTIGTGIGGGALQNGTFVEGFSHPEMGHMLV
                     RRHPVDTFKGVCPYHSDCLEGMASGPAIEQRTGMKGQELSVADPSWELEAYYIAQCVY
                     NTTLLFSPDVIIFGGGVMKQAHLRERVQEALVGLMNHYVDYPAFENYVLPPKLGDDAG
                     IIGCLALALEKSEKDIF"
     gene            complement(1509..2492)
                     /locus_tag="ACHBFA_002723"
     CDS             complement(1509..2492)
                     /locus_tag="ACHBFA_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9396135.1"
                     /translation="MAVKLTDVAKKAGVSPTTVSRVINNYGSLSQKTIDKVNQAMKEL
                     NYQPNSLARSLQGKNTQLIGLIFPTVANPFFGELVEQLESKLFDLGYRSILCDSANNK
                     EKERNYLNMLAANKVDGIIAGAHNLGIQEYENIELPIVSFDRYLADGTPIVSSDNFRG
                     GYLATENLYLKGARKIAILTGSEESNSPTNDRMNGYLKFMDEQQLEPQIFQFHSTARS
                     TALKNLEIKRILQNEEIDSIFCTDDLTAVLAYNLCQELDIRIPEQLKIIGYDGTKFIQ
                     NYFPHLSTIAQPITDIADILVDLLLQRIQDAEKPLESHYVLPVKLIQGKTT"
     gene            complement(2505..3983)
                     /locus_tag="ACHBFA_002724"
     CDS             complement(2505..3983)
                     /locus_tag="ACHBFA_002724"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9396136.1"
                     /translation="MKLKENWSRTDRYRPYKEWSEEHLHSLEKKINSSHWRLGYHIQP
                     VTGLLNDPNGFSYFDGKWQLFYQAYPMGSVHGLKSWYHLSSVNLIDWKNDGLKLIPDN
                     SFDSHGVYSGSALPIDNQLFLAYTGNVRDSNWGRHSFQLGAWMDKNGNITKIPTPLIS
                     SPPPGYTQEIRDPQLIAYDDGYLLIVGAQTEQKQGRILTYYSKDLQNWQFLGTLGFTE
                     EEMGFMIECPNLLFVNGKALLVFCPQGLSHEIHSYSNIYPNTYVIGQTFDFEKNELSD
                     PTHIKNIDEGFDIYATHCFSDENGRTLAISWIGLPEISYPTDEEGWAHCLSIVKELTI
                     EKEQLYQRPVKETAQLRKGKPYHFSGLETTIDAEINQYELQLEFDYSQKGTILLFADE
                     QQKHGLALSFDMIHGKMSMDRSHAGKSFAENFGTVREFSIEKKPLKLQIFVDRSVVEV
                     FVNDGQQTATARVFPTDDQTGLYIKSDREFKGKLWHLRNMNK"
BASE COUNT         1183 a          783 c          774 g         1301 t
ORIGIN      
        1 tagaagtgga aatgtataaa agatttattt tatgaagttt tcctaatatc attcactcat
       61 gatacgcagc tccgtatcat aagtgatgat atttttgtaa tgtgttcggg catgctctct
      121 acttactagt aggtcaagtg attgatcatt aaatagtaga aatagcatgt ctaaggagac
      181 attccacaaa atcattaaca tacattaaat aaattgagct gttgcataat agacataagg
      241 aaatgttctt ttctatattt agaaaagctg tcaaggcgaa gtttaaatat cacaaaactt
      301 aattggaaaa gagaatcaac tggaacttgc tagcgttgca agttgaataa aaaaacaacc
      361 aagattagat tgtaggaact tgtagaagat cataatttga agttaacaaa agaattccac
      421 gacacctcta ctctgttaag tacaggaaac agagagttaa gggcaagaaa aataggcgag
      481 gatatcgggt tgaaaggccg attggagaac ataagttaat agtaaataaa aagacgaaga
      541 aatcgcatca agtggattct tcgcctgctt attgatgaga ttgttgtgac aacgtgctta
      601 ttttattaga agatatcttt ttctgatttt tctaatgcta aagctaaaca cccaatgatt
      661 ccagcatcat ctcctaattt tggaggtaat acatagtttt cgaatgctgg gtagtctaca
      721 tagtgattca tcaaaccaac caaagcttct tggactcgtt ctctcagatg tgcttgtttc
      781 attacgcctc cgccaaagat gatgacatca ggtgaaaata gaagagttgt gttatagaca
      841 cattgagcga tgtaataagc ttctaattcc caagaaggat cagcgacaga caactcttga
      901 cctttcatgc ctgttctttg ttcgattgca ggtccagaag ccattccttc caggcaatcg
      961 ctatgataag gacaaacacc tttaaaggta tccacagggt gtcggcgcac aagcatatgt
     1021 cccatttcag ggtggctgaa accttctaca aaagtgccat tttgcaaggc accgccacca
     1081 atacctgttc caatcgtgta gtaaacaata gtggaacgcc cttttccatt ccccgcgaca
     1141 tattctccgt aagcagctgc attgacatcg gtggtaaaga caatcggtag ttcttttaaa
     1201 tttttattta aataaccgat aaaatcaaaa ttctgccaag ctttttttgg cgtagaggtg
     1261 atatatccgt atgttgctga attgggatga atatcaatcg gtccaaaaga acctattcca
     1321 atcgctgcca gttgttcttg gtgttttttg aagaaatcaa taacgttatt catcgtttct
     1381 atcggcgtgg ttgtagggat ttgtattctt tcaattactt ctaaatcttt cgtacctact
     1441 gcgcagacaa attttgttcc gccagcttca attgctccat ataacataaa acatttgctc
     1501 ctttgcagtt aagtcgtttt accttgaatc agctttactg gtaaaacgta atgtgattcc
     1561 agtggttttt cagcatcttg gattctttgc agtagcaagt ctacaaggat gtcggcaata
     1621 tccgttattg gttgagcgat agtagacaga tgcggaaaat aattctggat aaattttgtt
     1681 ccgtcatatc caatgatttt caattgctca gggatgcgga tatcgagctc ttgacacaga
     1741 ttgtatgcca atacagctgt aagatcatct gtgcaaaaaa tactgtcaat ctcttcattt
     1801 tgcaaaatcc gtttgatttc taaatttttt aaagcagtgg aacgagcagt tgaatggaat
     1861 tgaaaaatct gtggttctag ctgctgctca tccataaatt tcaaatagcc gttcattcgg
     1921 tcatttgtgg gggagttgga ttcttccgag cccgtcaaaa tagcgatttt acgagctcct
     1981 tttagataga gattttctgt cgcgagataa ccgcctcgga aattatcact gctgacgatt
     2041 ggtgttccgt ctgccagata gcggtcaaaa gagacgattg gcaactcaat attttcgtac
     2101 tcctgtatcc cgagattatg agcgccggcg atgatgccgt ccactttatt tgctgctaac
     2161 atgttgagat aatttcgttc tttttcttta ttattagcac tgtcacataa gatagaacga
     2221 tagcctaaat caaataattt actttccaac tgttcgacta attcgccaaa aaacggattg
     2281 gcgactgttg ggaaaatcaa gccgatcaat tgggtgtttt ttccttgaag tgaacgggct
     2341 aacgaattcg gctgataatt gagttctttc attgcttgat tcactttatc gattgttttt
     2401 tgacttaaag aaccataatt atttatcact ctcgagacag tagtaggaga gactcctgct
     2461 ttttttgcca catctgtcaa tttaacagcc attttcttca tcctttattt gttcatattc
     2521 cgcagatgcc ataatttccc tttaaattcc ctgtcacttt ttatatacaa gcccgtttga
     2581 tcatctgtcg gaaagacacg ggcagtcgct gtttgctgtc catcatttac aaacacctct
     2641 acaacggaac gatcaacaaa tatttgtaat ttcagcggtt tcttttcaat tgagaattca
     2701 cgtacagttc caaaattttc agcgaaagat ttgccggcat ggctgcgatc catactcatt
     2761 ttaccatgta tcatatcaaa ggatagtgct aaaccgtgtt tttgttgttc atctgcaaac
     2821 aataaaatag tacctttctg tgagtaatcg aattctaact ggagttcata ttgatttatt
     2881 tccgcgtcaa tagtggtttc caaaccgcta aagtgataag gttttccttt tcgcaactgc
     2941 gctgtttctt tcaccggtcg ttgatagagc tgctcttttt caatagtaag ttcttttacg
     3001 atgctcaagc agtgtgccca tccttcttcg tcagttggat agctgatttc aggcaaccca
     3061 atccaactga ttgccaaggt tcgtccattt tcatcactga aacaatgcgt cgcgtaaatg
     3121 tcaaatcctt catctatatt ttttatgtga gtagggtcgg aaagctcatt tttttcaaaa
     3181 tcaaatgttt gaccgatcac gtaagtattc gggtaaatat tactgtatga gtggatctcg
     3241 tgagataaac cttgtgggca gaagaccagt agtgctttgc cgttcacaaa taatagattt
     3301 ggacactcga tcataaaacc catttcttcc tctgtaaaac ccaaagttcc taaaaactgc
     3361 caattttgaa gatctttact gtaataagtt aggattcttc cttgtttttg ttcagtttgt
     3421 gctccgacaa tgagcaagta accgtcatca taagcaatca gctgaggatc acggatttct
     3481 tgtgtatatc ccggaggtgg ggagctgatc aaaggagtcg gtatcttagt gatattacca
     3541 tttttgtcca tccatgcccc taactggaag gaatgacgtc cccaattcga atcacgcaca
     3601 tttccggtat atgcaaggaa cagttgatta tcaatcggca aagctgaacc tgagtacaca
     3661 ccgtgactgt caaatgaatt atccggaatc agtttgaggc catcgttttt ccaatctatc
     3721 agattaactg aggagagatg gtaccaagat ttcagaccat gaacagaccc catcggatat
     3781 gcttgataaa atagctgcca tttcccgtcg aaataggaaa aaccattcgg atcgttcaat
     3841 aaaccagtaa ctggttgtat atggtagcct aagcgccaat gggaagaatt gatttttttc
     3901 tctaaggaat gcaaatgttc ttcagaccat tctttatatg gacgataacg atccgttcgt
     3961 gaccaatttt ctttaagttt catgacaaac tccttgtaaa cgtatttttg gaattataat
     4021 atattttttc tggtaaaatg t
//
LOCUS       DAYYNG010000198         4034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000198 DAYYNG010000000
VERSION     DAYYNG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.201"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACHBFA_002725"
     CDS             73..234
                     /locus_tag="ACHBFA_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM9396137.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACHBFA_002726"
     CDS             253..1530
                     /locus_tag="ACHBFA_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9396138.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACHBFA_002727"
     CDS             1540..2196
                     /locus_tag="ACHBFA_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9396139.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACHBFA_002728"
     CDS             2196..3572
                     /locus_tag="ACHBFA_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9396140.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="ACHBFA_002729"
     CDS             3702..>4034
                     /locus_tag="ACHBFA_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9396141.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DAYYNG010000199         4017 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000199 DAYYNG010000000
VERSION     DAYYNG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.202"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..972)
                     /locus_tag="ACHBFA_002730"
     CDS             complement(<1..972)
                     /locus_tag="ACHBFA_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFM9396142.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKE"
     gene            complement(976..1386)
                     /locus_tag="ACHBFA_002731"
     CDS             complement(976..1386)
                     /locus_tag="ACHBFA_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9396143.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1404..1895)
                     /locus_tag="ACHBFA_002732"
     CDS             complement(1404..1895)
                     /locus_tag="ACHBFA_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9396144.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1905..2696)
                     /locus_tag="ACHBFA_002733"
     CDS             complement(1905..2696)
                     /locus_tag="ACHBFA_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9396145.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2689..3462)
                     /locus_tag="ACHBFA_002734"
     CDS             complement(2689..3462)
                     /locus_tag="ACHBFA_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9396146.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(3618..>4017)
                     /locus_tag="ACHBFA_002735"
     CDS             complement(3618..>4017)
                     /locus_tag="ACHBFA_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9396147.1"
                     /translation="NKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKV
                     LYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKD
                     FFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1257 a          831 c          543 g         1386 t
ORIGIN      
        1 ttctttctct aacttaaaac ttgaaaatgg agtgatttct ttaaaattct caattatttt
       61 ttcaggcagt ttaacaccaa gagttttgtc tgtcttttga ataatttgat gtggaacaag
      121 tgcaccgccc caatcccagt tattttgatc gttctcccct tcttttgttg ccacccagcc
      181 agttagaaaa cggccagttt cattagttcc gactgttctt gctgcataat aggcttttcc
      241 gtcgaaagct tcgtctactg gtgatttcca atctgaaaat aatcctgctc cgactcgata
      301 tttagtcttt ttatcttcac aatactctga aaaaagtaaa taccacgttc catcaatttc
      361 aaatatatca ggcatttcaa tcatgttaaa ttcattatta gcccagtagt ctcctttgaa
      421 attccaatct tttaaatctt cggattcaaa atagaccaat ctccccgtcg gctttttctt
      481 acttgctggt tttctagctc ctagtatcaa aacgtatttt ccatgatctt gatcaaaaat
      541 tgcaaaagga tcgcgccaat cattcttatc atatccttct tgaggaacaa gttttgctgc
      601 ttcgccatcc tttttccaat gaattaaatc tttagaactt gcaatcatta acacttctga
      661 agtttttcca tccaacttag ctttgcgatt atgtccagta tacaaagcta aatactcatt
      721 gattccttgg acaatacttc cagcgtaaat gtattgatca atgtcatcgt tgccaccacg
      781 atgaattact tccccgtatt cttcaaattt cacaaaatca gtggttgtaa ctagtgacca
      841 cccaaaagga tcagttaaag gtttaggatt tctcgtatct cgttgatgaa atagataaaa
      901 agttccatct ttataaaaag gcatgcaatc cccaaaccaa aaatctttag gcttgtaaaa
      961 aattttttgc atttatcact catcctcttc gttagtatgt ctttgcatat ctgcaagaca
     1021 cgcaattgta ttcatatttc ttagattttc gatgatatca tcgctttttt ttccttgtaa
     1081 tctcaaattg acagcctcaa tcaaaattcc taaattcatg ccaccaagaa gttgaaaccc
     1141 attttgaaat acttcacttc ttttagcaaa ttcgttgaaa ggtgttccac caatcaagtc
     1201 agcaaatacc ataacttctt gattttttac ttgagaaact attttttcaa tgttttcacg
     1261 gaattcttca atgcctgcat ctccaagtgt taccacatga caatttaaat tatcgccaaa
     1321 aaacattttt aatgtatcta caaatccaat ggcaatattt ccatgagatg taattataaa
     1381 ctccatccac tcatcctctg ttcttaataa ataaaatcct ttaaaaattc ttttgaatct
     1441 ctaggagttt gtatcactgt cacttcaact ccatcactaa ttagttcttt aaaagcctct
     1501 ctctcttctt cagtaatatt tatcgtcgga cggatgcgtt ctgttccatt ccttcttgat
     1561 aaatttccta cgtttacact tgttattggt aaaccgttat caatcaaaaa tttaacatcg
     1621 attggagaaa ctgcaacaat aagaactttt tgtccttcat atttaccaga aagaatattt
     1681 ttaactgcta cttcccttgt aattattgaa gttctaacat taggtggtgc agcgaggcga
     1741 actacagctt tttttacgtc atcattggct acttcattat taataaccat aattctgtta
     1801 gcactcaatt gatttgtcca ttgtgttgct acttgtccat gaatcattcg atcatcaata
     1861 cgaacatgta taataccttt cattttttca tccatattca acacctactt caaaatattt
     1921 aacgctctta acgcaataga aactactaag attaatagaa ttacataaac tggtttaacc
     1981 tttcgtccta acaaataata cacaattgct actacacatg caggtaataa accaggcata
     2041 acttgatcaa agatttcatt tccggtcatt gttaagtcgc cttgtttaaa ttcaaagcca
     2101 acattcactt ttacaactgt ggcaatgagt gcaccgacca ccattaaccc taaaattgat
     2161 actgcatcag taatagactt taattttgtt tcaatttgag ttagtaggct cgcacccgat
     2221 tctttcccag caaggtataa aggataagca gtaatcctta ataaaattga tgccagtatc
     2281 cacaaaataa ttccaacaaa actaccttcc aatgccatat tagctgcaat tgctccaaaa
     2341 atagtccatg gaataactac aaatacacta tctccaattc ccgctaaagg acccattaga
     2401 cctgttttca gagcacttac tgcttccaaa gacttatttt tttcttcttt ttcaattcca
     2461 acattcatac caataatgta aggcgctgta tacggcgtag tgttaaaaaa ttgtaggtga
     2521 gctaaaacgg attgtttcat tccttcagga tcatctttat atgtgtcctt gataaaagga
     2581 agcatagaat acgcataacc caacccttcc attttttcat aatttaatga agttgcacca
     2641 ttgagaatcc accggaaaat tacttgtttg tcgatttttg tcagtttctt attcatcttc
     2701 gtcaaaacct cctgttgcag ctggtgctac ttgagtatta tttgatctat ttttatatac
     2761 gattagtgcg attgcagcgc caagtaacga aatccctaaa attggtattt ggaaataaac
     2821 tgccaaaacg aaaccaacta acaaataggc aaagtaatct tttgtaggta aatatttaag
     2881 tagcattcct atcccaacag caggtaatag tccccctgct acctttaaac caccggtaac
     2941 ccattcagga atataattga ttgcacttcc cactaacgtt gggcctaata tcacagttaa
     3001 caaaacagga attccagata caaagcttgt attaattgat gccaaatatt gcatccactc
     3061 taatttttga taatcttctt tttgtgcgaa tttttctgcc ttttgttgaa aaaaaatgtt
     3121 tgttgaccac tttagaacat ctagttgtac aattaacatt gctaccggta ttgctaaagt
     3181 tattcctata gaagcatcct ggttagttga gattgctaaa taagtaccga tcaatgctgc
     3241 tgtttgataa tccggtactg atgcaccacc aaaactagta atacctagtg tcattaattg
     3301 caaagtccca ccgatataaa gtccagtttg aatatctccc ataataagtc cggtcaagaa
     3361 acctgcaacg acaggttggg aggttccgat tttgggccct tccttatccc agttaatgaa
     3421 aaatccatac aacacaacta aaaatgtttg ccaaaacgcc attttgttct ctcctttctt
     3481 cttatacttt tatataagca attactgtgc caactaacat tatccaataa agcgggaaaa
     3541 tacagtaaat attaattaaa tgagcgtcta aatatacttt ggacaccctt tcattttttt
     3601 agacgctagt atgctattta gaaaatagtc tgctcaaaaa ttgtatagat taactcatac
     3661 tctcctttgg gtatttcaat acagtaaata tctttgattt ctgaaaaaaa atcttttgat
     3721 acatttaaaa aagtatcaaa tagttgcgct ccctctttca aatgttcatt tgttattcca
     3781 tcaccaatca taattcgttc aatcattcca gaaagatgta gaaatagatt aattctcaga
     3841 aaacttggga ttttgacatc gtaatatttt tcatacaaat atagtacttt ttctatctca
     3901 ttgaatactt ttttggaatc taaaaagcga agcttagtac tagcgccttc aatagtaaag
     3961 agtttaacta attcatttaa gcagaactca cagctatccg agtccatata cttattt
//
LOCUS       DAYYNG010000200         3991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000200 DAYYNG010000000
VERSION     DAYYNG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.203"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..353)
                     /locus_tag="ACHBFA_002736"
     CDS             complement(204..353)
                     /locus_tag="ACHBFA_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396148.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            815..1027
                     /locus_tag="ACHBFA_002737"
     CDS             815..1027
                     /locus_tag="ACHBFA_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396149.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1305..3104)
                     /locus_tag="ACHBFA_002738"
     CDS             complement(1305..3104)
                     /locus_tag="ACHBFA_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9396150.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3228..3824)
                     /locus_tag="ACHBFA_002739"
     CDS             complement(3228..3824)
                     /locus_tag="ACHBFA_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9396151.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1430 a          708 c          686 g         1167 t
ORIGIN      
        1 tcgaaacagc taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt
       61 ttgtttaggt ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag
      121 acgtttgtcg cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt
      181 cgccatttgg aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg
      241 attgtaatac caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta
      301 gaagttgtat caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc
      361 atctcctttg taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc
      421 aagtttagca tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta
      481 ttttttctta aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg
      541 ttacaaatac tattgcttga atataaatca tatgctcacc tctttataaa taacttagca
      601 atataaagaa aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg
      661 tctaagatcg tgttaaaaaa tagttatcta aaaagcagaa agatactcaa aataaaaaat
      721 aaaaaaatgg ataaaaacgt tttcaaatac aaggtaattt gttattataa aataaagaaa
      781 aaacaaaata aaaataaaga aaagagggaa tgaaatggta aaagcagaag taggaagatc
      841 ttatatatgc aaaccgatcg gagtatctca tgaactaatc ggctgtattg aacgtacata
      901 tatcaacacg gctgtcgtgt atgttgaaag ttatgaccaa cgagactatc aacttatcaa
      961 tgaaagcaag tatcgcatgc tcgtaaaatt ctgtgatatt agcgcacctg ctgaattggt
     1021 ttcttaaaca gtatttttga gaattcgcaa gaactccctt ataatataaa taaagacaga
     1081 agggagcgag aagatatgcc aaaaagaaaa agttcaggga aaatgggaat tattcttgca
     1141 gtgatctctg caattcctgc tattttagct attgttcgtg cgatgaagga atcaagagaa
     1201 agttaagcac acattatgag cgtcaaacaa aaaacgcata tacctgcggt ggcaggttat
     1261 gcgttttttg ttgtataaag acttttttca ttaacgattt ttgattagcc gactcccaaa
     1321 acaaaattta gcagtttatc tgaataagtg atttcttcat ctgtttgttt cacactttca
     1381 tttagtagtc ccatctcatc ggttatgata ccatccacac cataaaacat catgcgactc
     1441 atagcatctg aatcgttgat tgtccataca tatacttctt ttccgtcttg gtgggcagaa
     1501 ttaatgaagt tacggttgat cgtactatat tccattgtca gaaaatcagc aggcgtcaca
     1561 gggggcccaa caatattgaa aggtaagatg tagcccacat aaaagtttgg ttctgcttct
     1621 tttaaatctg aaacggtttg gtacgtcaaa ctttgcaaaa tatgatggtt ctctaagata
     1681 actttgcggt atttatggac aaatcgttcg acaaggtcat ccgaatcttt tttagaggtc
     1741 ttgatctcaa taagtaattt ttggtttagc ttatgtgctt tttccaaata agaatcaaaa
     1801 gaacaaacag gggcttgcac tccgttttca gtaactgtaa gatttgtcaa ttctttcaat
     1861 gtaagatcct gtggtgttcc atttacccct gttaagtgac gaagatttgc atcatgcatc
     1921 actacgaaat gaccatcttt tgtttcctga acatccatct ctacataatc aggatgatag
     1981 tctctgcttg ttttttcaag tgcttctaaa gtattttgcg ccccattttg ggtactcaca
     2041 cctctgtgag agacagtcac tggttttgtc tgaacagctg aagtcagata atccatattg
     2101 taaagacaga cgccaatacc gaaaaaaact gcaaaaagag tcaaaccagt gtttttcagc
     2161 gcagagaaac gcaagttggg ggcttgtggg ataaaccact tagggatctc aggcaagaag
     2221 ccttcgtcat ccatgaaatc aacaatgata taaaaaatcc ctacggtaga catcacgata
     2281 ttgaataata aaatcccttg taaaagcgtc atagcaaaca cagcgctgat caatgcatag
     2341 tctgggaaaa attgttcaac catactttgt gccaaaataa caacgatata tcctaaagaa
     2401 gaaaggagta aaatcgtacc gccaatcaca ataaattggc ccgtgatggc caaaagacgc
     2461 gatttggtca atgaccagct ttcccgaatg gctgctcgaa aaggacgatc tcgtagaatc
     2521 atttccggta aagcaaaaat cagtcgaatc ccaatatagc caaggaaaat ataaacgagt
     2581 aaaaaggaac tgacgatgat ccaacgatta gcaaaaataa aatccataat gaaagcgggg
     2641 atttttattt ttgacagtag atcagaatta aaacttaatc cgctgatcgg tagaatcaat
     2701 aaaaaataag caaggaaaaa caaaaaagta atgggtctca ctttcttcag ctgaagtatc
     2761 gtcaagtgta agagttgctt caaagagatt ggttctttct ttttaataaa aaaaacactc
     2821 atcaaaagaa aagtaaattc gaaataaact aacagtaaaa tcgtcatcaa aactacgatt
     2881 aacgagagta gaaccccagg atgttgagaa aaaattgaag gaatattgtc ataagacaaa
     2941 tagtcaattg cccctcggtg taaaataaaa cgggtcgtac tgctcaataa gggtaaacaa
     3001 ataaataaaa taaagccatg catcagcaag acatcacgga aataagcttg tgttcctttt
     3061 aaaaagtccc aagcattacc taagctacgt ttcaaataat tcatcctatc acctgctctt
     3121 cacattgtag cgcaaatcta atgaaaaagg aaagggtgca gtgtgttttg cagacccttt
     3181 cccagattta acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga
     3241 ccacttagat attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt
     3301 ttagcaatca aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc
     3361 aaacataacg catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca
     3421 aaacggtttt gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg
     3481 gaaagtatga atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg
     3541 cagattccaa tatagttata gataaatcca gcataagggc caaatatcag gacaccagct
     3601 gcagtactga tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca
     3661 aaaataatcg gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct
     3721 ttgaaaacac ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata
     3781 atggatatga aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt
     3841 agaggttctt tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata
     3901 tgacagaaaa caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa
     3961 acatccaact aaagacttat ttaataactt g
//
LOCUS       DAYYNG010000201         3909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000201 DAYYNG010000000
VERSION     DAYYNG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.204"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..3149
                     /locus_tag="ACHBFA_002740"
     CDS             117..3149
                     /locus_tag="ACHBFA_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFM9396152.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3339..>3909
                     /locus_tag="ACHBFA_002741"
     CDS             3339..>3909
                     /locus_tag="ACHBFA_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFM9396153.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1343 a          622 c          827 g         1117 t
ORIGIN      
        1 taataaatca aatgattgat aataagatag ctgaattgat ggaacttgag agttataaaa
       61 aagctctcat ctacgaatac gtaaccggta aaaaagaagc ctaggaggtg attcatttgg
      121 tagagaacaa agaaattcgt tttgagcagg atattgagac ttcactgact acgtatggca
      181 aatggaagaa agaaacattc aatgaatcaa actttgatcc cataattggt ttggattgtc
      241 aaaaattgct aggctttgtc cagaagactc aaccaaaaca gtgggcacgt caccaacgaa
      301 actataacaa tgatggagaa gtcaagttcc ttcaacgctt taatcaagag gtagaaaccc
      361 acggcatgtt acatgtattg cgcaatggga tcaaagaccg cggtgtgcgc tttaaagtag
      421 cttacttcaa acctgcttca gagctgaatg atgaaacgat ggaaaagtat gaagcaaata
      481 gttttacctg tactcgccag tttgcttaca gtcctaataa ccataaaacc attgatatgg
      541 ttttgtcgtt aaatggtatt ccgctcgttg ctttagaatt gaaaaaccag tacactggtc
      601 aaacagtaga aaatgccaaa ctccagtttc aaaaagatcg tgatccaaaa gaattggtat
      661 ttcgttttaa tcgccgcttc ttagtctatt ttgcggtgga ccatacagat gtttatatga
      721 cgacaaagtt ggacggtaaa aaaacgtact tcttgccatt caatcaaggt tccaatgggc
      781 cgggaagagt tggtggagca ggcaatccgt ccaataaaga tggctaccca actgcctact
      841 tgtgggaaaa ggtgcttact agagacaatt tgatggatat tatccaacgt tttatgaatc
      901 tagagattaa aaaagaaaag caaggcgaca gagaagtcac aaagaaaacg ttgatttttc
      961 ctcgttatca tcagttagat gtggttcgaa aattggttgc agatgtaaaa agccatggta
     1021 gtggagaaaa ttatttgatc cagcatagtg ctggttctgg aaaatctaat agtattgcat
     1081 ggctggcata ccatttagcc tctattcatg atcaactgaa ctacccaatt ttttcatcaa
     1141 ttattattgt gactgatcga acagtgttgg atcgccagtt gcaaaatact atcatgaact
     1201 ttgatcatca gacaggtttg gtagaaacga ttgacgatcg caaatcctct caagatttaa
     1261 aagaagccat taatgatggg cgtaagatca ttattaccac tttgcaaaag ttcccagtga
     1321 tctatcagga agtagatcaa gtggaaggtc gtaattttgc tgtgattgtg gatgaggcac
     1381 attcttcaca aactgggaac agtgcccaga agctaaaagc cgctctttcg gacaaatcta
     1441 aatccttgga agagtattat gaactggaag gcgaagttga cgatgtatta ccagatgctg
     1501 aagatgaact agttcaacaa ttggtagcac aaggtaaaca tcaaaattta agtttctttg
     1561 cttttaccgc tacaccgaaa gaaaagacat tggaaatgtt tggtacgaaa caaccagatg
     1621 ggcatttcga accctttcat gtttactcga tgcgccaggc gattgaggaa ggctttatct
     1681 tagatgtgct gaagaactat atgacctata gtacttccta caaaattgcc cgtgaaattg
     1741 aagataaccc agaattacca aagagtgaag caacccgagc gattgttcgt ttccaatcat
     1801 tgcatccttg ggcagttact caaaagacgg ctgtgatggt tgagcaattc cgagcggtta
     1861 ctcgtacagc tattggtggg caggcaaaag caatggtcgt taccgcatca cgcttacacg
     1921 ctgttcgcta tatgaaagaa tttaaaaaat atatagcgaa caaaggttat aatgacttgg
     1981 atgtgttggt tgctttttct ggttcggtag tagatggaga gactgaatat acagaaacga
     2041 agatgaatgt cacaaaatct ggtgagaatg ttcgggagaa tcaattaaag gaagctttcc
     2101 atacagataa ttttaatatc ttgattgttg ctgaaaaata tcaaacgggt tttgatgaac
     2161 cgttgctgca taccatgttt gtcgataaga agttaaaggg tgttaaagct gttcagactt
     2221 tatcacgact caatcgaaca atgcctggga agaaggatac ctttattctt gattttgtaa
     2281 atgatgttga agatattgaa gctgctttcc aaccctttta tgaacaaaca aatttgagag
     2341 aagaaatcaa tgttaacttg atttatgata ctcaaagcaa gctacgaaaa tttaatgttt
     2401 acaatcaagg tgatattgaa cgtgtcatga aattggtgaa acaagcccaa aagcaacaga
     2461 atgagcgctt gcttggacga atttctagca tgtttaagcc gattatggat cgctatgagg
     2521 aaatgaacaa ggataatcag taccagtttc gggtgactct acgtaacttt ggcaagtggt
     2581 atgattatat ttcacaactt gatcggttat tcgatatgga actattggag gaacgaattt
     2641 ttactggata cttgctgaag tttctaccta aagaaacacg agaaaatatt gatatttctg
     2701 acaaagtaaa gttggaatat tataagttac gtcaggactt ttccggtgaa atccatctag
     2761 tgaataaaga aggcgagcta gtaaatccaa agtcaattga tgcaggaatc aaaccgccgg
     2821 atgaacgaga ttcactagaa gaaatcattc ataaaatcaa tgaacgattt ccaaatgatt
     2881 ttgacgaagg agaccgggtg ttagtagaga ttttgtatcg gtcatttgcc gataacccag
     2941 atttgaaggt tgtcagcatg gctaagaata acgatgctca gatgtttgaa aaatcgctgt
     3001 tccctgatgt atttaaagat aaggtgatgg agcagtacga gaagaacaat gcagcttttg
     3061 agaaaatgtt tgggcatgat gataagtact ttgatctagt atatacatta gttgctagag
     3121 atttatacaa gttattacgg acgaaataaa atttataaat aaggattaga caatacaatt
     3181 tctctcaaaa ataaaggaga agttgtattg tttgctttta agaaagccct gaaaaatttg
     3241 ttacgtacta aattgtctga actctgtcat atcagctatg tcagaataaa ggaaatagat
     3301 ttatttttat aaacagtgtc gtctcggaga tttaaactat gtcaacgaca attgaagtga
     3361 acaaacaaag cgtgaagcag tttcttgaaa cagggaaaat caaaaaattc gttattccag
     3421 aatatcaacg tccttatgcc tggactgacg agcaaaacca agtactattt gatgatttag
     3481 cggagtacac agcaaacaat aacgagagta cttacttttt aggaagcatt gtggcttatg
     3541 aaaatgatca taacgagcaa gaaatcattg atggacagca aaggataact acgctatttt
     3601 tatttttgag agctatctat gctaaattag aaaatagttg tgaaaaggaa gctttattct
     3661 tgaaaagcca aattgaaccc gcgttatggg aacaagatga tttaacaggt gaggttaaac
     3721 cagataaaat tctgattatg tctcgagtaa tgggggatga ggggaatgaa gaatttgcta
     3781 gtattctagt aagtggtgaa gcagatgtta aatcaaaaag taattattcg aagaactata
     3841 ttttaattca acatctatta aatgagtatg caacaaacga accactatca ttttatcgtt
     3901 ttatcagta
//
LOCUS       DAYYNG010000202         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000202 DAYYNG010000000
VERSION     DAYYNG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.205"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHBFA_002742"
     CDS             complement(717..3866)
                     /locus_tag="ACHBFA_002742"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM9396154.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYNG010000203         3838 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000203 DAYYNG010000000
VERSION     DAYYNG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.206"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..333
                     /locus_tag="ACHBFA_002743"
     CDS             <1..333
                     /locus_tag="ACHBFA_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396155.1"
                     /translation="VLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSI
                     SLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNN
                     FYFYRNKL"
     gene            complement(459..1136)
                     /gene="nth"
                     /locus_tag="ACHBFA_002744"
                     /pseudo
     CDS             complement(459..1136)
                     /gene="nth"
                     /locus_tag="ACHBFA_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1143..1841)
                     /locus_tag="ACHBFA_002745"
     CDS             complement(1143..1841)
                     /locus_tag="ACHBFA_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9396156.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2109..3293
                     /locus_tag="ACHBFA_002746"
     CDS             2109..3293
                     /locus_tag="ACHBFA_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM9396157.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3299..3742)
                     /locus_tag="ACHBFA_002747"
     CDS             complement(3299..3742)
                     /locus_tag="ACHBFA_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9396158.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          998 a          759 c          636 g         1445 t
ORIGIN      
        1 gtacttttaa gcactactct taaaggaaaa tttatggaaa gaagctctca tgcaatgatt
       61 ctcacttttt tatggttgtt gttcaccaga atctatattt ttctgatgag cctgaaaccg
      121 cccaaatcca ttagtttatg tttagttata aatggtattt ttttattatc acttatcatt
      181 tttattacac ctttagatag atacgtcact tatttatata atcctttgga atactggact
      241 tattttatag gtatattgga aggcattatt ttctacatcg ggtattttcc caataaaaat
      301 aacaactttt atttttatcg caataaattg taataagtta ccatcctagc tactgagtat
      361 gtacatttac tttttcatgc aaaaaagata aacgaaacag cttttctcca taataacaag
      421 ataattcata catttatttg caattaaaga aaccacgtct acaggcgtgg tttctttttt
      481 agcgcctttt cttttagacg catccgattt ttcccatctt gacatattga tagcaaagga
      541 catacctcgc acttgggatt tcttgccgtg caatgatatc aaccaaaaaa aatcagtgta
      601 tgatgtgtct ttacccataa ttcttgtggt actttccgca tcagtgtttc ttctacttcc
      661 atgaccgtag catctagttt gcagatcctc aggcgttttg agacacgttc cacgtgggta
      721 tctactgcga ttgctggtat cccgaatgca tcccccaata cgacatttgc tgtctttctg
      781 cctacaccag gaagagacat caattcttct cttgacgttg gcacttgccc atcgaaacgt
      841 tcaatcagct gctgtgcaca cgctttgatg ttttttgctt tatttcgata caaaccaatc
      901 gtttttattt taagaatgat ttcatcgatt gaagcttctg ctaacgcatc gggtgtcgga
      961 aaagaggcaa acaaatctgg cgttgcttta ttcactgata catctgtcgc ctgggcactt
     1021 aagatcactg caatcaataa ctcaaatgga ttattgtgct tgagttctcc atgtgcttcg
     1081 ggaaacattc catacatcgt ttctagagct tccattgttt tttgtttgct taacatattc
     1141 cgctactcct ccaaccaatt tttcagtgag actttcggca attcttcttg ttgagctgac
     1201 tgttctactt ctctttgaag aagctgctgt tttcttcgtt tttggtcttc ttccacttgt
     1261 tgttttgtac gtagattttt tctttcccac gatagcaaaa tgcggtcaac gtaattaaaa
     1321 ctataagcct gatttaatac ggcttcccgc agtgctagct tcaaaatttc cggctgataa
     1381 tggtcttctt ctagccattg tccgatccgc tggaactcga tcgaagaaag cgggcgtcca
     1441 aattcttgtt caaataattg atacactgac tggatttctt tttcttgtgt tttttcttcg
     1501 tatttttttt cttgtgtttt taaatactca cctaatagat catagatggg gtaaagatta
     1561 tatcgatctg cttttttacc gttgacaatc gtcgtttcta tcttgataaa tccatttgtt
     1621 acgagacgat ccagtatttg aaaaatctga tcttgtttga ttcccatatc catagcaatc
     1681 atctgcaaat cagggaaagt atctccttca agctgtgcca tatgcaactg tagaacaaat
     1741 aaaaattctt cagattgtag accgatccta tgataatttt taagtaaaag attagatatg
     1801 actgtttgtc cagcgcttaa gtaattttct aaattcaaca tgatttctcc tccgctcaat
     1861 cacagtatac gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta tttacttcac
     1921 tgatcggtca gatttacaat gtcgtctact tgatttttgt ttgttcatgt ttttgatgcg
     1981 tatcttcact tttcggaata aataaattaa gtagatttcg ctgttcctta gaatgttgac
     2041 aaggaaacaa aattactata cactattttt gtttccaagt taacattatt gcataaagga
     2101 gtttttacat gtacagtttc acaaataaac attctaaagt ttttggatac gtctctgtct
     2161 tattcactgg attaggattt actatcgtca gtcctgtact tccgttttta acgctgcctt
     2221 attctcactc catacatcag caagctttct atatcacttt gctcatgtct gtttatgctc
     2281 tagctgcctt cttatctgcc cccattctcg gtagcttaag tgatcatttt gggcgtcatc
     2341 cgatccttat tatcagttta ttaggatcaa gtatcggata tttgcttttt ggtttaggaa
     2401 attcgatatg gatgttgttc cttggtcgta tcattgaagg attgacggca ggagaaatct
     2461 ccaccttata tgcttacttt gccgacatca cggaacctaa tgaaagaacc aaagtttttg
     2521 gatggatggg tgctttagtc ggtataggaa caacgctggg tcctattata gggggtctat
     2581 tagctgagtt ggggaattct gttcctatat ttattggagc actctttact ttcttaaatg
     2641 ccgtttatgg ctacacattt atgctagaaa gccttccaat aaaaaaacgt tcagtagatt
     2701 taagtttctc ccatgtaaga ccttttcacc aattacaaca attattcaag atttcatccg
     2761 ttattccttt attgacagca ggttttgctg tttggttggc agctggctct cttcaatcta
     2821 tcttttctca attttcgatt gacgcctttc agtggaaggc tgggttggtt ggtttaagct
     2881 tctctttgat tggaatcttg gatactgtct cgcaattgtt cattatgcct cgattgctca
     2941 aaaaattttc agaacagcag atcacaagaa tagggatgtt cagtgaaatc ctcgcctatc
     3001 tattcatcac attatctgga attcttctac tgccgatttt gtttttattt gggattatct
     3061 gttatggttt cggtgattct atttttactc ctgtattcaa tggccaacta tccaattctg
     3121 tctcagataa ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa tctctatcgc
     3181 gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc agcaccattt cttccgacgg
     3241 tattcggatt tttgcttatg atttttgcct attatcacta caataaaaaa tagtagcttc
     3301 atttgctact tcgtataaat tctctgtatc gaccgataaa acgaaatact gtgtcaaatt
     3361 cttcttttga catattttca aatacagtag catctcgctg tacccatttt tgatggagtt
     3421 gctcgtgtct attccttatt ttttgtccga ttggcgtcaa ttgaaaatag atttcttttt
     3481 tattctcttc gctttgataa gtgtttatga tccctctagc tatcagtttt tttgttagct
     3541 tactgatggc tcctctagtc atgtaactag cattcgctaa ttttgtcacg ttaggatcag
     3601 ggattttccc gatatgttca atcacttcta gttcatttag agtaaatcct gcaaattctt
     3661 tttccatttc tggtttatta cgatgaataa tctcattagc taaatccaga tattcttcta
     3721 ttagcagttg tcttttatcc atttctacac cttctttttc tttcattata ttgttttttt
     3781 gtttcctatt caacaaagat aaaaatattt tgtaggaaaa cccaatctga aaccaaga
//
LOCUS       DAYYNG010000204         3821 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000204 DAYYNG010000000
VERSION     DAYYNG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.207"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBFA_002748"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBFA_002748"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9396159.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHBFA_002749"
     CDS             complement(2260..2511)
                     /locus_tag="ACHBFA_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9396160.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBFA_002750"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBFA_002750"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM9396161.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHBFA_002751"
     CDS             3461..3679
                     /locus_tag="ACHBFA_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396162.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1059 a          812 c          712 g         1238 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt g
//
LOCUS       DAYYNG010000205         3763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000205 DAYYNG010000000
VERSION     DAYYNG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.208"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..523
                     /locus_tag="ACHBFA_002752"
     CDS             <1..523
                     /locus_tag="ACHBFA_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396163.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2053
                     /locus_tag="ACHBFA_002753"
     CDS             584..2053
                     /locus_tag="ACHBFA_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9396164.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2064..>3763
                     /gene="topB"
                     /locus_tag="ACHBFA_002754"
     CDS             2064..>3763
                     /gene="topB"
                     /locus_tag="ACHBFA_002754"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9396165.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1392 a          638 c          832 g          901 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc
      241 tgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt
      361 aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaattca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgct gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg
     1081 tttagatcat gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc
     1141 aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca
     1201 cattgacaag aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca
     1261 tttaaaattc tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa
     1321 actacaagat gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta
     1381 cgttgaagag tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat
     1441 atgtgtggac aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat
     1501 cgttgatcct tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca
     1561 agtacctaaa gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc
     1621 tgaagcaatt atggccatac acaaaactga aactaattta cagctgacaa atcaattggt
     1681 gtctgctcat gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga
     1741 aacaattgat ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg
     1801 cgcagatgga acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt
     1861 taatgcagga atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca
     1921 tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat
     1981 ccagcaacaa gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca
     2041 agaaagggag tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag
     2101 cgaaggctta tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc
     2161 aagatcgatt gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt
     2221 tagattcgcc cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta
     2281 ttttccccaa tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg
     2341 tcaaacgaca gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag
     2401 gggagctgat tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta
     2461 aaagactttg gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac
     2521 gagacgcaga agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt
     2581 ggttaattgg gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac
     2641 caggaagttt ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc
     2701 aggaaaagat tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg
     2761 ctcaaaatgg cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag
     2821 catttgaagc ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc
     2881 ttcaagtcga aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc
     2941 ttcagtctaa aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc
     3001 aagggttgta tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg
     3061 aaggggaata tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg
     3121 aagcgattcc gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc
     3181 aggaacatta cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac
     3241 taagtccact gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg
     3301 cggaaaagta tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa
     3361 aggcaatcga aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg
     3421 acggaaaaga gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga
     3481 cggtggatct acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta
     3541 cgttgatcac tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca
     3601 tattgaaaga agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct
     3661 tgaaacaaaa ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga
     3721 ttttgctttg ccaagccgtg gaaaaagagc cgctgcttac gag
//
LOCUS       DAYYNG010000206         3754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000206 DAYYNG010000000
VERSION     DAYYNG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.209"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..523
                     /locus_tag="ACHBFA_002755"
     CDS             <1..523
                     /locus_tag="ACHBFA_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFM9396166.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2044
                     /locus_tag="ACHBFA_002756"
     CDS             584..2044
                     /locus_tag="ACHBFA_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9396167.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2055..>3754
                     /gene="topB"
                     /locus_tag="ACHBFA_002757"
     CDS             2055..>3754
                     /gene="topB"
                     /locus_tag="ACHBFA_002757"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9396168.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1383 a          647 c          825 g          899 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc
      241 cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt
      361 aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggcaaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 aacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgcc gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg
     1081 tttagatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc
     1141 gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca catataagca
     1201 tatagataaa aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca
     1261 gttaaaattt tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca
     1321 gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta
     1381 ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat
     1441 ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc
     1501 agtcgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca
     1561 agtgccaaaa gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc
     1621 agaagctatt atggccatac acaagacaga aaacaatctg caattaacga accaactggt
     1681 ttccgctcac aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga
     1741 agccattaat ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg
     1801 cgtggatggt acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca
     1861 gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga
     1921 atttatacct gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca
     1981 agaaatcaga aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga
     2041 gtgaaatcgt catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt
     2101 atgccgatag cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat
     2161 tgtttcctgg tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc
     2221 ccgatatgta cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta
     2281 cacattatca ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc
     2341 aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga
     2401 tcgcttggac gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt
     2461 ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa
     2521 aagagaccta tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg
     2581 ggatgaacgg tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct
     2641 tttccttagg cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga
     2701 ttagaaattt caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg
     2761 gcgcatttga tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag
     2821 attatttaaa agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg
     2881 aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta
     2941 aaatgaatca gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt
     3001 atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattacg gaaggggagt
     3061 atgcgtattt gttggatcat ttagatgaat acaagcattt tttaaaggcc gaatcaatcc
     3121 caacccctat tcatacgccg aatagtcggt atgtgaataa caagaaagtc caggaacatt
     3181 acgcgatcat tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac
     3241 tgcagcaggc catttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaaat
     3301 atacctatga agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg
     3361 gaaaagtacc gatagatcta ggttggaaga agttatttgg caaagagaac gacggaaaag
     3421 agaaggaaga agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc
     3481 tacaagtcct tgaaaaagaa accaagccac cacagcctta taccgaaggt acgttgatca
     3541 cggcgatgaa aacagctggg aaaaccgttg atagtgaaga agcgcaatcc atattgaaag
     3601 aagtggaagg aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa
     3661 aggaatatat caaggtagag aaaaataagc tcgtggtgac gaataaaggg attttgcttt
     3721 gccaagccgt ggaaaaagag ccgctgctta cgag
//
LOCUS       DAYYNG010000207         3747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000207 DAYYNG010000000
VERSION     DAYYNG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.210"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..352)
                     /locus_tag="ACHBFA_002758"
     CDS             complement(188..352)
                     /locus_tag="ACHBFA_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396169.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            647..1975
                     /locus_tag="ACHBFA_002759"
     CDS             647..1975
                     /locus_tag="ACHBFA_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM9396170.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2177..3010
                     /locus_tag="ACHBFA_002760"
     CDS             2177..3010
                     /locus_tag="ACHBFA_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM9396171.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3026..>3747
                     /locus_tag="ACHBFA_002761"
     CDS             3026..>3747
                     /locus_tag="ACHBFA_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9396172.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANI"
BASE COUNT         1241 a          594 c          782 g         1130 t
ORIGIN      
        1 gcgcatgact gttaatcatg atgtcgtagg ttcgagtcct actgccggag taaataaagc
       61 ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt attatgatga
      121 atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta gagttggcta
      181 ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc attcctacac
      241 caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta tctgtattca
      301 taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc attttattca
      361 tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa gctatcttat
      421 tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt atctcataga
      481 aatattcctt tttatctgaa aactattgta taccgtatga catttttctt catgaagttg
      541 taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt tctaaattct
      601 taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga aagctattac
      661 gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac agcaggcatt
      721 gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt cagcacatgt
      781 ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta atcaaatgca
      841 atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga tgctgaatca
      901 attccattta aaagagtttg aattatcaca aaattacact tttttctggg ataaatatga
      961 aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa tcgatagccg
     1021 gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg atatgatcgt
     1081 ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa gtgcgaatag
     1141 ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag atgcagtagt
     1201 gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa agaaagaaga
     1261 aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc caaaagaatt
     1321 cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac tgacaccaca
     1381 aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg tgatcaatgc
     1441 accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag gaaatgtcgt
     1501 tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa aattagcgat
     1561 tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc aatcttctac
     1621 tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat ttgatacgga
     1681 ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga cccatgcgat
     1741 ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag tcgaaaatag
     1801 ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct ggatggacga
     1861 atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat taaatgcgtt
     1921 caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag catagacgag
     1981 tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca gctcttttta
     2041 tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa ataaaggata
     2101 aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa taaaattggg
     2161 agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat taaatggtaa
     2221 agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa ccgaacatcg
     2281 tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag cagaagtaga
     2341 cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc taggtgaaac
     2401 aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg atcaacgaga
     2461 tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg gttttcgtaa
     2521 agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt ggtggacttt
     2581 tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga atcgatggaa
     2641 tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat cggttgaacc
     2701 aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg tgttctttga
     2761 ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc gggaacaaga
     2821 tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg cttatcaaac
     2881 tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt tatattatct
     2941 tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc aattactaga
     3001 aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc atgaagtttc
     3061 atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag gagtagttat
     3121 caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg tcaatagctt
     3181 tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc ctagagtcgg
     3241 tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct gttatcggaa
     3301 cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg ttttcgtatt
     3361 gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg ctccttttga
     3421 gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg attcgggctt
     3481 catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg ttaataatgc
     3541 gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta cgcatgttcc
     3601 agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga tcgaaagcca
     3661 ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc gaatgcaaga
     3721 acagctgtat tgtgaagcaa atattat
//
LOCUS       DAYYNG010000208         3714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000208 DAYYNG010000000
VERSION     DAYYNG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.212"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..551
                     /locus_tag="ACHBFA_002762"
     CDS             <1..551
                     /locus_tag="ACHBFA_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9396173.1"
                     /translation="FQTYALTNAGASQTQATGLGIILNVVALGTTIVFTSISGGKYRN
                     KAFFVGAFVQFAAMLGMVLIGGGAGFIALATTIAFYNFGNPLAGEAIYKVWTQESFPA
                     ETRASLQGFINGFSRLCCGLFAFVTPYLVMPDHIQSSMIGFAWIVLVATIAGIVMMRL
                     QKKYGTSETRGTEDLQDTNVTA"
     gene            579..2165
                     /locus_tag="ACHBFA_002763"
     CDS             579..2165
                     /locus_tag="ACHBFA_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9396174.1"
                     /translation="MAFTFQINNDVVWDYDQKLLKKAEENIPKLIETEIQPIRIVEKI
                     PAQQRTNEFKLQDSQKDICTLAQYSLGRGESIILDLGDHYVGQFSIDIDSVGSPMDAP
                     LYLRLKFAEVPSELYARSEDYEGWLSRSWIQEEYLHLDTLPTTLQLPRRYSCRYVELT
                     VIDTSPKWQASFSSPKFIAKSSVSMAQVPTMTIEDNTLKAIYDVSVKTLLDCMQTVFE
                     DGPKRDRRLWLGDLRLQALANYYTFNDLSLVKRCLYLFGGMRTNEGKIPANVFTEPKL
                     TPDDTFLFDYSLFFVTSLYDYVAHTKDKNVLADLYLVAKKAIDLALKQVDIEGRLVLT
                     EDFPVFVDWSNDFDKATAGQAIMIYTLKRFLKLAKLSNEANEMYYQEILEKMINFSNQ
                     TLFNHEKKQFVIEKNNEINVASQVWMVLAEVFDKQTNKEIMLETIKYHFPIQGIATPY
                     MYHHVVEALFIADCQEEAIKLMKEYWGKMIELGADTFWEAFKPEDLTFSPYGSPILNS
                     YCHAWSCTPAYLIGKYLIGK"
     gene            2283..3656
                     /locus_tag="ACHBFA_002764"
                     /pseudo
     CDS             2283..3656
                     /locus_tag="ACHBFA_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343224.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1147 a          576 c          741 g         1250 t
ORIGIN      
        1 agttccaaac gtatgcttta acgaatgctg gagctagtca aacacaagca actggattag
       61 gaatcatttt gaatgttgtt gctttaggta caacgattgt ttttacttct atttctggtg
      121 ggaaatatcg aaataaagct ttttttgtag gtgctttcgt tcaatttgca gctatgctcg
      181 gaatggtttt aatcggtggg ggcgcaggtt ttattgcttt ggcaacgacg attgcttttt
      241 ataattttgg taatccttta gctggggaag ccatttataa agtatggaca caagaatcat
      301 ttcccgcaga aacaagagca tcattgcaag gatttatcaa tggtttttca cgattatgtt
      361 gtggactttt cgcatttgtt acgccttact tagtaatgcc agatcatatt caatcttcga
      421 tgattggctt tgcttggatc gttttagtag caacaattgc tggtattgtc atgatgcggt
      481 tacaaaagaa atatggaaca tctgagacac gagggacaga agatcttcaa gacactaatg
      541 tcactgcttg aaaaattgac caagtaagga gaataaatat ggcatttact tttcaaataa
      601 ataacgatgt agtttgggat tatgatcaga aattattgaa aaaagcagaa gaaaatattc
      661 caaaactaat tgaaacagaa attcaaccaa ttcgaattgt tgaaaaaatt cctgcacaac
      721 aacgtaccaa tgagtttaaa cttcaagata gtcaaaagga catctgcaca ttagctcagt
      781 attcactggg aagaggtgag tccatcatct tggatttagg ggatcattat gtcggtcaat
      841 ttagtattga tatcgattct gttggttcac cgatggatgc acctttgtat ttacgcttga
      901 aatttgcaga agtaccttct gaattatatg cccgttcaga ggactacgaa ggctggttgt
      961 cacgttcctg gattcaagaa gaatatcttc atcttgatac gttacctact acactgcaat
     1021 tacctaggcg ctacagctgt cgttatgtag aactaacagt tatcgatacc tctcctaaat
     1081 ggcaagcaag ttttagtagt ccaaaattta tagcaaaaag ttctgtttca atggcacaag
     1141 tacctacgat gacgatcgaa gacaatactt taaaggcaat ctacgatgtt tctgtaaaaa
     1201 cacttttaga ttgcatgcag accgtctttg aagatggtcc taaacgagat cgaagattat
     1261 ggttaggcga tctacgtctt caagcactag ctaattacta tacttttaat gatttgtctt
     1321 tagtcaaacg atgcttgtat ctttttggcg gaatgcggac aaatgaaggg aaaatacctg
     1381 ctaatgtttt tacagaacct aagttaacac cagatgatac gtttttgttt gattatagcc
     1441 tcttttttgt tacttcctta tatgattatg ttgcgcatac caaagataag aatgtcttag
     1501 cagatcttta tcttgttgct aaaaaggcaa ttgatttagc actaaaacaa gttgatattg
     1561 aggggcgttt ggtactgact gaagattttc ctgtctttgt cgattggtcg aatgattttg
     1621 ataaagcaac tgctggacaa gcaatcatga tctatacatt gaaacgattt ttaaaactcg
     1681 cgaaactatc gaatgaagcg aatgaaatgt actatcaaga aatactagaa aaaatgataa
     1741 acttttcaaa ccaaactcta tttaatcatg agaaaaaaca gtttgtcatt gaaaaaaaca
     1801 atgaaataaa tgttgccagt caagtttgga tggtgttagc cgaagtattt gacaagcaaa
     1861 caaataagga aatcatgcta gaaacgatta aatatcattt tccaattcaa ggaattgcga
     1921 caccttatat gtatcatcat gtggtggaag ctttatttat agccgattgt caagaagagg
     1981 ctataaaatt gatgaaagaa tattggggaa agatgattga attaggtgca gatacatttt
     2041 gggaagcttt taaaccagaa gatttaacct tttctccata tggtagccca attttaaata
     2101 gttattgtca tgcatggagt tgtacaccag cctatttaat agggaaatat ctgataggta
     2161 aatagttagt taattaggaa aaatacatgg atcagttctt acttgttaat gaattgatcc
     2221 gtgatttaag aaaaaatgaa agctgttatc tctgttggca gatatagtaa aggaggctta
     2281 ttatggaaac aacacaaatg agtctattaa aaagaaagtg gttgacaaaa tggcgacaac
     2341 ggattggtta tggggtatct gacttcgcat gtaatcttat ctggcaagta atttcgctct
     2401 atctcttata tttttatacg gatatcatga atttgaacgc tgcagcaatc agcctgatgt
     2461 ttgtagtgac gcgttttgta gatggtgtga cagatttatt ggttggctac ttgatcgatc
     2521 acacgaatac acgctgggga aaatcacgac cgtattttct ttttggagca gttccttttg
     2581 cactttttgc ttttttgtgt ttcaatgtgc ctgatttttc taattcagga aaattagtgt
     2641 atgcatatgt cacatatctt ggtttgtctt ttgcatatac attagtaaat gtaccaatgg
     2701 catcgatttt gccaagtttg acaaatgata cagaagaaag aacggcacta tcaacttctc
     2761 gaaaattttt tgctttccta ggtgcaacga ttgttagttc atctgttttg acacttgttg
     2821 ctgtttttgg taagggaaat gaacaattag gcttcaagat tgtcatggga atgtttggcg
     2881 taatcagctg tctactattc ttttttacat tctccgctgt tcgagaattg cctgtccatg
     2941 gaacaaaaaa tccttcattc aaaagggtct ttacgtcttt gaagcaaaat aaaccttgga
     3001 tgatctttgc agtcaatatc ctgtttatgt ggaccagttt ttttatttaa tcaagtgcac
     3061 tagtttatta tttttctgta gtgattggaa gcaagacact ttctattact gtagcgacga
     3121 ttatgtcaat tgttcctatg tttgcaaact tttttgttcc tatacttgct aaactattag
     3181 gcaaaagaaa tttatatctt ttatccgcaa gtatccagtt attaggattg atagttatca
     3241 tgtttgctgc taagaatata acactgatac ttgttggtac ctttatttca gccattggtt
     3301 acggagtaaa agaaagtatt tatttttcga tgcaagcaga tccagttgat tttggtgttt
     3361 ggaagacagg tgtgaatgtt tctggttcac tttctgcaat taatggcttt ttagggaaag
     3421 ttgcacaggc aatttctggt ggtgtagcgg gtgccttact tgcttgggga atgtatcaac
     3481 ctggagaaat cagacaatcc ggaaaggcaa tttttgccat tcaagcgatg tatctttatg
     3541 taccaatgct cttgattatc tgttcgatag taacaatgat gttttatcat ctagataata
     3601 tttatcccca aataaaggcc gatttggaaa tgaagaaatc tttggatgtc aaatgataag
     3661 aaaagtaact tagatgagaa tactaagtaa caagaggttg tgacttgttt ttag
//
LOCUS       DAYYNG010000209         3689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000209 DAYYNG010000000
VERSION     DAYYNG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.213"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..489
                     /locus_tag="ACHBFA_002765"
     CDS             <1..489
                     /locus_tag="ACHBFA_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396175.1"
                     /translation="LGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGF
                     ILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVT
                     VKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKV
                     VI"
     gene            533..802
                     /locus_tag="ACHBFA_002766"
     CDS             533..802
                     /locus_tag="ACHBFA_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396176.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1034..1330
                     /locus_tag="ACHBFA_002767"
     CDS             1034..1330
                     /locus_tag="ACHBFA_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396177.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1332..1754
                     /locus_tag="ACHBFA_002768"
     CDS             1332..1754
                     /locus_tag="ACHBFA_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396178.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1773..3365
                     /gene="mobP2"
                     /locus_tag="ACHBFA_002769"
     CDS             1773..3365
                     /gene="mobP2"
                     /locus_tag="ACHBFA_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFM9396179.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3386..>3689
                     /locus_tag="ACHBFA_002770"
     CDS             3386..>3689
                     /locus_tag="ACHBFA_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396180.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1466 a          524 c          728 g          971 t
ORIGIN      
        1 ttaggcagta tgttaacgac tctaacagat cggttcgaac aatctgaaaa aacggtagag
       61 gggataaaga acttcttaac gacgattgat caatttataa atggatatat gtatttgttt
      121 gaagtaggct ttattctcac tttgtttggt tgtgtattta taaaactcat taaaagatca
      181 atcgaaaaag aaatggttat tgatctaatt ccgacgctga gcggctatga gtattacgtt
      241 gattttggaa agtatgagga gttgatccat acggatatcg ttcacacgag tatccatgaa
      301 gtaaccgtta agggcatcag atctacttta aaagatggac aggagacgaa aacaatcgac
      361 caaagaaaag aggtctacaa tttttataaa gttgaagcaa gtctacgata tggcattggg
      421 aaattgaggt tagatgggag taatattcaa caaaacaaac catatgtttt aggaaaagtg
      481 gtgatttaga acttcctatg aaagatgaat aaataaggag aatcttttga aaatgattct
      541 tctttacaga attgtgctta tagttggcac ggagttaaaa atcttaaaga aggagcagtt
      601 attcaaagtg aaagaacatc ataatcagtt actatgtatt aaaacgggta cgatgaacat
      661 tcaataccga acagaatatt tagaattaac gaatcaaact gattttttgt cgattcgaga
      721 gcaacataag caagaaaaat taatggctac ttatagccaa ttgattgatg agtgggaaca
      781 attcaaggaa tctaaaacat aaaattccat ttaaacacct tattttttaa agagcataag
      841 gaaacgtgac cccactttta aaaaaagtgg ttggataggc tgtgcctatc acgtttctga
      901 aaaaaataaa tgaccccaaa ataccaatat ataggatagt acaaggatag caaaggatag
      961 taaaaggata gaggtaggat agcgaaaagg ataggatcct tttcactatc ctatattata
     1021 ttggaggata tttatgaatg ttaaaattag agatttagat ccaaaattca tttctgaaat
     1081 tgatcgaaga tgcgttgaac taagtaatcg tacaggaaat aaatggagtc gtaatgatta
     1141 tttaaagcta ctcgttgaaa atgattttga tcgtcctttg atggaataca aacaagaaaa
     1201 gtttgaccag ttattagaaa agttttcaga aattcaaagt tataacacaa agatattaga
     1261 agaatatgtt agccaaaata atcgtattat agaaatcttg attgagcaaa atagaggaag
     1321 tgaactttga tatggcaaag gttatagaca aacacattta ctttcaccaa acacatatcg
     1381 atgtattgga tgaaactatt tctactcatg cagaggttaa aaactatagt gaagctgttc
     1441 gctttttatg tatgaactat gatccagaag tggagaaaaa agtaaattcc aatcaagcaa
     1501 agttgaatgg aatgagtaaa gaaatttcta ttttaaccga aatggtttca gaatttgcgg
     1561 atcttcattt gaaagataca ggaatattaa ttggtaaaaa ttcaactgtt tatcaagaag
     1621 caaaagaacg agtggaagga aaaattaaat ccaatcaaac aaaaaaatat tcccctcagt
     1681 tgaagaaacc tgtgaatgaa tctgttcatg ccagagtaga tccatttgat ccaacgaaga
     1741 ttctaaaaag ataaaaatga aggaggataa taatgactca atcgccttca gttattctca
     1801 cttgtcaatt tactttgccg aataaaaagt cattttctac ttatattgac tatattacaa
     1861 gggaacaggc tcttgaagaa aaggaacgac gtacgcccga agaagaacag gaacttcaac
     1921 gaatcaagaa tgctttagag gagttttata ttcctgaagg agaaactttt tcggagagta
     1981 agacgggtaa agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa
     2041 tcaattttga caagttagag gaacaagatt ttaccaatta tctttcttat atgacacgtc
     2101 actatgcgtt agaacaaaag aaagaactga cgcaaagtga acaaaaagaa taccaacgct
     2161 tgaaccaagc aatgaaaaaa tatcaaacag aaccccataa aaaagcttca ggaaatcttc
     2221 ctggagtttt ttctatagct tcagacgaag tgaaaggaag tgatctgaag gagattaagc
     2281 aaatcttcca aagaggacaa caaaaaggct caatcattta tcaagatgtt gtttctcatg
     2341 ataattcgta tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag
     2401 aagggttaaa agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga
     2461 atgaaacagg ttactggatg gcaacgattc accgtaacac taaacatatc catattcatt
     2521 ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac ggaggttgaa aatggtgtga
     2581 gttatatcgt accgaaaggt aagagaagac aagcaacatt agacgcaatg aagaccaatt
     2641 atgtacatga gttagaacgt ttttcttatg aaaaggatcg aatgattggt atggagaaac
     2701 gaaatttatt gacgcggaaa agtgatttgc gtaacacctt aacaaaagtg gtaaaagagc
     2761 cagcgcgtta cgatcgacac gcgatgaact tattaaatga agtatataaa accctacctg
     2821 acaaacgttc ggaatggaat tacggggatg aaaaacgaac caaactttca ccatacacaa
     2881 gagagaaact cgatgagtta accaaatatg ttttaacaaa agaacctgaa tatcaagaat
     2941 atatacagat ttcaaaagca ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga
     3001 gagaatcaaa ggatgcagag aaaaatgctc tctttgattt aaaaaagcgc acaggaaatg
     3061 ccatcctaac agagctgaag agacaggata gtcaaataaa accattaata gaaagtgttg
     3121 atcagatgca atctatttac aatgaacgat cctttcgaac gcttaaacaa ttgccaacga
     3181 atgaatacaa atatagttac gaagaattca tgaagcgcag gaaacaattc caacaaagag
     3241 ttgtcacgca gcggagtttg cgtcaattaa atcgaacgat taatgcccat gaagaaaaat
     3301 accacgcaag taaagcatac gaagagcaac aacgaagaat tcaaatggaa caagaaagac
     3361 agtaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga aagttataga
     3421 aggatcatta agagaagaac gaatcgaaca acttcctgat tgtttagaaa aagagcattt
     3481 gttccagata agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt
     3541 tgagtaccca gctactggtg tgtattgttg taatcatgtg aatggtgaaa aatattttat
     3601 cattcaagaa tatgagggca agttagcacc ttattatact acttgggaaa tgaatgaaga
     3661 gggaatcaat aactttccat gtaaaagca
//
LOCUS       DAYYNG010000210         3663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000210 DAYYNG010000000
VERSION     DAYYNG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.214"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACHBFA_002771"
     CDS             <1..276
                     /locus_tag="ACHBFA_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFM9396181.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACHBFA_002772"
     CDS             419..640
                     /locus_tag="ACHBFA_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396182.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACHBFA_002773"
     CDS             656..1498
                     /locus_tag="ACHBFA_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396183.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACHBFA_002774"
     CDS             1561..3363
                     /locus_tag="ACHBFA_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFM9396184.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="ACHBFA_002775"
     CDS             complement(3422..>3663)
                     /locus_tag="ACHBFA_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM9396185.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAYYNG010000211         3640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000211 DAYYNG010000000
VERSION     DAYYNG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.215"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1960
                     /locus_tag="ACHBFA_002776"
                     /pseudo
     CDS             4..1960
                     /locus_tag="ACHBFA_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
     gene            1950..3545
                     /locus_tag="ACHBFA_002777"
     CDS             1950..3545
                     /locus_tag="ACHBFA_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9396186.1"
                     /translation="MKNKWCKSAVIYQIYPRSFQDTNGDGIGDIPGILSRLDYLENLG
                     INAIWLSPVYESPNDDNGYDISDYRKIGAEYGTMADMDLLIKEARQRGIRIIMDLVIN
                     YTSDRHPWFQLAKKSVTSPYHDYYVWRKEPNGLESNFGGSAWTYVPEVNQYYLHLHSK
                     KQPDLNWQNPKMRQAIYDMMNFWLEKGIGGFRLDVIDLIGKQPEQSITKNGPLLHDLL
                     QEMYQKTFGHYDVVTVGETWGATPKIAQLYSKEERKELSMVFQFEHINLDKQNGKRKW
                     DLKDLDPQELHRTFSKWQIELGGCGWNSLFWNNHDLPRIISRWGDDQEYRTISGKMLA
                     IYLHFMQGTPYIYQGEEIGMINYPVKSIDEVDDIESQRMYAERIKKGYSKEELIQSIN
                     SKGRDNARHPMQWNNHKNGGFTEGTPWLPVGNTEEINVEAALAAQDSLFYTYQKLIEL
                     RKELPVIVYGKYQVIETEEPNVLAYLRSLKKQKILVVVNFSDQKTTFPIGKYRVKQVL
                     IQNYKKKITNNLLPYEAFSALIY"
BASE COUNT         1156 a          606 c          829 g         1049 t
ORIGIN      
        1 gaaatggatt acaaaaaagt agctgaagaa atcagcaaag cgcttggaga agacaatgta
       61 caagcagctg cgcattgtgc aacacgttta cggcttgtct taaaagattc agacaaagta
      121 gaccagaagg ctctggatga taatgaaagt gtgaaaggaa cttttgaagc aaatgatcaa
      181 tttcagatca ttatcggagc aggtgatgtg aataacgtat atgatgaatt cgttaagatc
      241 accggcgtaa aggaagcttc aaccgacgat ttgaaagcgg tagcatcgaa taagaagaat
      301 aatactgtca tggcctttat caaggtgttg tcagatatct tcgttccgat tgtccctgca
      361 ttggttgcgg gtggactgtt gatggctctg aataatgttt tgacggcaca ggatttgttt
      421 ggtcctcagt cagtggttga gttgtatcca tcagtgaaag attttgctgg catcattaat
      481 ttacttgctt cagcgccgtt tgcttttttg ccgattttgg taggtttttc agcaactcga
      541 cgttttggag gcaatccgta tcttggagca gcaatgggga tggcgatggt catgccggat
      601 ttagtgaatg gatacagtgt gacgaatgct attgcagaag gatcgatgcc ttattggaat
      661 atttttggaa tgaatgtggc acaagccggt tatcaaggat ctgttcttcc agtccttgca
      721 gtttcttgga ttttagctac tttagaaaaa ttcttccata aacggttggc taatgctttc
      781 gactacacat ttactccaat gctagctatt attattactg gttttttaac ttttacagta
      841 gtagggccgg tgatgcgtac tgtcggagac ggcttagctg atggattaag ctggttatac
      901 agtactactg gagctttcgg cttaggtgtc tttggtttct tctattcgcc gatagtcatt
      961 accggattac atcaaagctt tccagcaatc gagactactt tactagcaga tgttgccaga
     1021 acgggtggct cttttatctt tccagttgcc tcaatggcaa atgtagcgca aggagcggct
     1081 gctttggcga ttatgctgct gacaaaaaat aaaaagcaaa agagtctagc taccactgca
     1141 ggtgtttccg ctatgttggg gattactgag ccagcgatat tcggtgtgaa tttgaaactg
     1201 aaatttccgt ttatttgtgg aatgattgct gcaggaattg cttcattctt tatcggcttg
     1261 ttccacgtat tagcagttgc gatgggaccg gcaagcgtca ttggctttat ttccattgca
     1321 cctcgttcga ttccttcatt catgatcgga attgttatca gtatagtgat cggttttttt
     1381 aagcacttac ttctatggga aaaaacatat ggaactgcta gcagatggtg aatcaaaagg
     1441 aacagaagca gctgagtcaa tcagttcagc tcctaaaacg actaggaaac cgcaagatga
     1501 aattttagct gcatcagtca gcgggaagat tctcccttta acgaaggtaa aagatccagt
     1561 tttttccact gaaatgatgg gaagaggtat agcagtcaaa ccagatgacg gggtagtcca
     1621 tgctccagca gatggtatat tgacagttgt ccacgaatct aaacatgcat atggcttgaa
     1681 aacagagaaa ggagcggaag tactgatcca tatcggtatc gatacagtga atctaaaagg
     1741 ggccggattc acgacacagc gacaatatgg tgaattagtg aaaaaagggg atattctcgg
     1801 tacatttgat cgtaaattgc ttaaagaaga agggtacgac gatacaatta tggaaattgt
     1861 cactaattcc tctgcatatg ctaatgtaaa aattcttgaa aaaaacacgg tcaaaagtgg
     1921 tgaagatatt ctgatagtga gtgaaacaga tgaaaaataa atggtgtaaa agtgcagtaa
     1981 tctatcaaat ctatcctcga agttttcaag atacaaatgg agatggcatt ggagatattc
     2041 ctggtattct ttcaagatta gattatctag aaaacctggg aatcaatgcg atatggttaa
     2101 gcccggttta tgaatcacct aatgatgata atggctatga tatcagtgac tacagaaaaa
     2161 taggcgcaga atatggaacg atggcagata tggatttact gataaaagaa gcacgacaaa
     2221 ggggaataag aatcatcatg gatcttgtta tcaattatac gtcagacaga catccatggt
     2281 ttcaattggc aaaaaaaagt gttaccagtc cctatcacga ctattatgtt tggcgtaaag
     2341 aaccgaatgg attagagtcg aacttcggcg gttcagcatg gacctatgtg cccgaagtga
     2401 atcaatatta tctgcacttg cattcgaaaa agcagccgga tttgaattgg caaaatccaa
     2461 agatgagaca agcaatctat gacatgatga atttttggct tgagaaaggg attggcggat
     2521 ttcgattaga tgtcattgat ctgattggaa aacagccgga acaatctatt actaaaaatg
     2581 gtcctctttt acatgattta ttgcaagaaa tgtaccaaaa aacttttggt cattatgatg
     2641 tagtgacagt gggagagact tggggagcaa cgcccaagat tgcgcagctg tattcaaaag
     2701 aagaacgcaa agaattatca atggtctttc aattcgagca tattaatcta gacaaacaga
     2761 atggcaagcg aaaatgggat ctaaaggatt tagatccaca ggaattacat cggacttttt
     2821 ctaaatggca gattgaatta ggtggttgtg gttggaattc tttattttgg aataatcatg
     2881 atctcccacg aatcatttct cgctgggggg acgatcaaga atatcgaaca atcagtggaa
     2941 aaatgctagc gatatacctc cattttatgc aaggtactcc ttatatctat caaggagaag
     3001 agatcggcat gatcaattat ccagtaaaga gtattgatga ggtagatgat attgaaagcc
     3061 aaagaatgta tgcagaacgt ataaaaaaag gctattcgaa ggaagagttg atccaatcaa
     3121 tcaactcgaa ggggcgagat aatgctcgcc atccgatgca atggaacaat cacaaaaatg
     3181 gtggctttac ggaaggcact ccttggttgc cagtggggaa tacagaagag atcaatgtag
     3241 aagcagcatt agctgctcaa gattccttgt tttatacgta tcaaaaactg atcgaattgc
     3301 gcaaagaatt acctgtgatt gtctatggga aatatcaagt cattgagaca gaggaaccga
     3361 atgttttagc atatctacgc agtttaaaaa aacaaaaaat actagttgta gttaattttt
     3421 ctgatcaaaa gactactttt ccaatcggta agtatcgtgt caaacaagtc ttgatccaaa
     3481 attacaaaaa gaaaattact aacaatttac tcccatatga agcttttagc gcgctgatct
     3541 attaagtggt tttttctata gatgaatctt tgttatgagg tagatagttc aatgaaacag
     3601 ttattcgatt tgtttataga aaacaattta atcagcaaaa
//
LOCUS       DAYYNG010000212         3624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000212 DAYYNG010000000
VERSION     DAYYNG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.216"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..425)
                     /locus_tag="ACHBFA_002778"
     CDS             complement(27..425)
                     /locus_tag="ACHBFA_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9396187.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(422..658)
                     /locus_tag="ACHBFA_002779"
     CDS             complement(422..658)
                     /locus_tag="ACHBFA_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9396188.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(818..1102)
                     /locus_tag="ACHBFA_002780"
     CDS             complement(818..1102)
                     /locus_tag="ACHBFA_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396189.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            complement(1273..3501)
                     /gene="topB"
                     /locus_tag="ACHBFA_002781"
     CDS             complement(1273..3501)
                     /gene="topB"
                     /locus_tag="ACHBFA_002781"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9396190.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT          963 a          723 c          591 g         1347 t
ORIGIN      
        1 attgagaaat aaagttttta attcgttcat tttattgcgg tttgtcgtaa ataactggtt
       61 atcttggact ttagtgaatc gaaatccaat gaactagtgg taatcattat gataaaatca
      121 acagcttctt gacgagaaaa agcaactgga caattattca attgaagaaa caacaacatg
      181 gcaacaagag cagttcgttt attgccatta tgaaaaggat gttttttagc taaattaatt
      241 gctaaaatcg ccgctttttc atagatttca ggatagagtt cttgattgaa tactgcttgt
      301 gaaggctgtt tgacggccat atctaatgca tttgcatctt ttacaccgat catttcgcct
      361 ggcgagtgac gtaaaataac tttcgcgttg attttgataa tttctttagt cgaaagatac
      421 ctcataaatc aaccaaatct tttaacgcgt cttcatattg attcatgatc gaatcaacta
      481 atgaatcaat atcaggttct tcttgtgctt tgatcattcg tacagaacca tcagattgaa
      541 aaacataact gattgcgtca cctgtctgca cacccatgaa ttcttttact tctggtggga
      601 tagttgttac atcgctattg ccagattttc taattttata ctttttcatt tttttcatat
      661 ttcgccaccc tctataccat tctcattcta tagtaagtac attatatcca agtatatact
      721 tttaatcaat gtatatactt ttattttgct ttttcttacg aaaaaatgac aaagaaaaaa
      781 gtttgtgaca tgacttttgt cacaaactct cgtattctta accaaaatat tgatgaaata
      841 cttgtgtaat aactggtaga aaataaataa tgatcatagc cgctaccacc accaacaatt
      901 gttttcgctg tttctttcgc tttagcatgc ttgtcgtttt atgaaaaaaa taagtagcgc
      961 ttttagctcc tgcagtcaaa gtcgataaaa tcaacgttgg tgaatatgct gtttgattgt
     1021 gcgtcaatga caacaacttc gtcccgtaat caggatactt tagaaatgct tggtacacag
     1081 ccacaatcgt gattgctccc ataagataat ttaaaaatga atgcactttt ttcaaataaa
     1141 aaaactcctt gctatctata taaaatcagc ctaccccaac gtttttgctt gattcactga
     1201 tattttttag ttttattggt atttatattc tcacttttcg gaataaatat tccgctattt
     1261 cattgttttc atttactcta cttcgttttc attatttttt cttgatgttt ctttgtttcc
     1321 atcaactgct tttgttccac tgttttcgat ccatggaaaa gaaaaaccga ttgtcatatc
     1381 ttcttttaat actaaaactg catcgaattt ccctttttta ccagtaaaac ctttgacaac
     1441 tttacttgtc tttccttttg aaagcaaatc tctgagattc gttttagtca gcttcttttt
     1501 agctactgtt ggccacaatt tgaagtcgca ttccggattc aagcatgtag cgattttagg
     1561 ataaagcatg actattccct ttttacattt tggacatctt cctaattgat cgtttttctt
     1621 ctccatcgct tttatctgac gtgtcccgct aatctcatct gaaaagtcta ccttgtcgat
     1681 ttgattagga acttctgcca ataaatgtag gatgaatttt ttgatattct ctaaaaaaac
     1741 agatggtgtc ccctcttgtt taccaatttt tctcaagtac ccttcccagc gcgcagtcat
     1801 ttctgcgctc gtcagtaatg gttcattggc tactgcctta caaagagtga ttccttttgg
     1861 cgtaacagtt atttcgtttt tattgacttc gatgtaatgc ttctgcttaa gattttcaat
     1921 gatattggct cgtgtagctt ctgtccctat tccttctact tctttgagaa tttcttgcgc
     1981 ttcatcatta tccagcgttt ttcccgcagt tttcattgct gtgatcaacg ttccttcggt
     2041 atagggtttt ggaggttgtg tttctttctc catacttttc agttctactt ctgcttcttc
     2101 attttctacg ataggaggaa gagtctgagc ttctttcttt tttgacttga tgcgtaagat
     2161 ttcctgccaa ccttgctgta atgggatctt tccgatgctt tttagcttta attgctgtac
     2221 gcccgtcatt acggtcgtct cttcgtagat ataatctgca gcaaacatag ccacagtagt
     2281 gcgcaaaact tgcaaataga ttgcttgctg taatttggaa agcttcgcaa agcgtgcagg
     2341 agtcggaatc gtctttgtcg gaataattgc atgatgttct tgaacttttt tcgcatcgac
     2401 atatcgtttc tttgctttga tctcagttgt ctgtacttct tctattccta aaaatttttt
     2461 ataattttgc agatgtttaa ccagatactg atgctcattt tctgtaatgt aaaaggaatc
     2521 cgttcttgga tagcttagaa acttgctttc gtacaatcct tgagcagcat caagtgtagc
     2581 tttggcactt gctttcatta gctgattcat ctttgtctgc aaactagata gcgaaaataa
     2641 acgtggacta tgcaacctct tttccttttt cgtaacttca agaatccgtc cgttttgctt
     2701 acctaaatgc gctccaagtt tttctatttc cttcgttaac tcttcttggg ttttaaatgc
     2761 tttttttggt acgatttttc cagaaaactg ccctttgttt gtcgtaatta aaccttctcc
     2821 ttcaaagtac gtttcttttt tgaagtgttt gatttcttct tgcagctgat aaatcatata
     2881 gagtgtcggt gtttgtacac gaccaagaga aaaactgcca tcaatccctt ttcgttgaag
     2941 tagcaagcta tataatggac ttccattcat tccaatcagc caatcagcgt tctgccttgc
     3001 ttgcgcttct ttatattttg gaaaatagtt ctttccaggt tggagattct taaaaccttc
     3061 atagattgct tctttttcaa gagaattgat ccataaacgt aaatagttct tttccttaga
     3121 aaatgcgttt gcttgtcgaa taatcgacca cgcgatggct tcaccttctc gatcaccatc
     3181 tgttgcaata atgatggtat cggctgactg caattctcgt tttacgatcg aaaattgtgc
     3241 acgtttgtcc attggaactt cgtaacggaa ggtttctggg aaaataggca aattttctaa
     3301 agaccatttt gcccaacgct cttcataagc tcctggagca accatatcta ccaaatgtcc
     3361 aaaaccataa gtgatggtta cttcaccagc aaacaatgga tcgctgattt cataataccc
     3421 ttcatgccgc ttttgctgtt ggaaggtttc ggcataggct tttgcttgtg aaggtttttc
     3481 agctagtatc accgtcttca tcttctgacc tccgttactt aattttttgc ggacttctct
     3541 attatggaac atattttcgc atctggcaat aaaaaaaaga taccgtatag tcgaaataga
     3601 cacaaaaaca tcaagcaaac aaac
//
LOCUS       DAYYNG010000213         3530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000213 DAYYNG010000000
VERSION     DAYYNG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.218"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..2208
                     /locus_tag="ACHBFA_002782"
     CDS             55..2208
                     /locus_tag="ACHBFA_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM9396191.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2394..3203)
                     /locus_tag="ACHBFA_002783"
     CDS             complement(2394..3203)
                     /locus_tag="ACHBFA_002783"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9396192.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         1170 a          630 c          731 g          999 t
ORIGIN      
        1 aagaagtttt tgggtacaat gaagcatgaa tgtaaatagg cggaaggata ggaaatgatg
       61 aaagatttta tgaaaaaaat attaaatact tcttggttag aaaaaataag aaaaggtgca
      121 caagaaccta agaacaaaac gtttcgccgg tcccatgttc cttttcgctt aaattttctt
      181 tttttcatta tatttacgtt atttgttgcg ttgattgcta gattgggtta tttgcagatc
      241 gtgaacggcg aagagatgga ggcgcgagtg aagtctactt ctacgattac ggtccaagaa
      301 agtactcctc gcggaatgat ctacgactcc acaggaaaag ctttagttac gaataaagcg
      361 aatcaggcca tcacatatac gagaggaaca caagttacgg ctaaagattt gcttgaaatc
      421 gcaacgaagc tgaactcgct gatcgatgtt ccggtagatg aaaatctgac agatagagat
      481 aaaaaagatt tttggctggc tgatcccaaa catttgaaag aagccacaaa aagactttcg
      541 gcaaaagaaa agcaattagg gaacagcgaa caatatagtg cgatggtcaa taaagtcaca
      601 gctgatgaga ttaatttaaa cgaggagcaa ttaaaaattg ctaccatctt taaacggatg
      661 aatagtgcat atgaattaaa cactgtctat atcaaaaact ctggtgttac tgatcaagag
      721 ttagctgttg tggcagaacg cgcttctgaa cttccaggag tttcgactgg cacagactgg
      781 acacgagaat ataatgcagc aagttcactg aaaagtattc ttggcagtgt gacgactgaa
      841 aagcaaggac ttccagcaga tgaagcagag aaatacctag caaaaggtta ttctcggaat
      901 gaccgtgtag gacaaagtta tttggaaaaa caatatgaag atgtcctgca aggaaccaaa
      961 acccaatatg aagtttccct tgataatgaa ggaaatgttt cgaatcaaaa agaaatcttc
     1021 tctggggaaa aaggttctaa tttaatgtta tctatgaatg cagaattcca atcaaaagta
     1081 gaagaaattt tgaaaaggaa ttatcaaaca ttgatcaaca acggcaaagc acagtattcg
     1141 cctggtgctt atgcagtggc tatgaatcct cagacaggag aagtgctagc aatgactggt
     1201 ttcagtcatg aacaaggttc caaagaaatc accgagaatg ctttaggcac gattactagc
     1261 gcctttgatc cagggtctgt agtcaaggct ggtactttga cagcaggctg ggcaagtaac
     1321 gcgatcagcg gaaatcaagt tttgattgat gagccgattc gtctgcaagg tgcttcagaa
     1381 aaaagctctg tctttaatcg aagcggacaa gttgctcttg atgcagtcaa agcatcggaa
     1441 ttgtcttcta atacgtatat gatcaaagtg gcgttaaaga tgttaggact agactataca
     1501 cctggaatgg gactacctag tttagatgaa gaagctaaag cttatcagca attgagagac
     1561 tctttcaaag agtttggtct tggtacaacg actgggatcg atttgccgaa tgaatctcct
     1621 gggatctcac gttctgttga ttatatgaag aagttcaatg cggataatgg caaagaatgg
     1681 tatgctccag gtaactttac cgatttggca tttggacagt ttgacacata tacaccaatc
     1741 caattagctc aatatgcttc aacagtcgct aatggcggga aacgagtaca gccgcaccta
     1801 gtcaaagcga tctatggcaa tgacgaaaat ggaaacttgg gtgaagtgaa gaaagaaatt
     1861 ggcacaacag tagaaaatac ggtcaatatt tctgcagaaa atatggcgat cctccgtgaa
     1921 ggattccacc aagttgtcca tggtaccgat ccttacacaa cagccaaacc attagccagt
     1981 gcgaaaatgg acctttccgc aaaaacaggg actgcagaaa cggttgcgga aggtcatcca
     2041 gatattgcga cagtcaatag taatatcgtg gcatacggac caacagacaa tccggaaatt
     2101 gccatcagtg ttgtcttgcc gaacttatta gacgaacaag accacataaa cttgacgatt
     2161 gctaaagaaa taatggacgc gtattatgat atgttcatgg caaaataaaa aagaggtcgt
     2221 gaaaaagccg cttagtttcg agtatgaaga aaaattttgt gaaaatagct tctcacattt
     2281 ttgcttaaat ttgacttcat accgagaaac tggcttttga acaccgttta ctcggcaaaa
     2341 aaggttgtga caaaaattgt cacaacctct tttttgtgga atctaattct ctattatagt
     2401 ttaagtagtt tttccagata aacagctaaa ccgtcttcct gattgctcaa aggagtgaca
     2461 tcatttgcaa tgcctttgag ttggtcagta ccgttagcca tagctactcc ccaacctgca
     2521 taatccaaca attccaaatc attatgctcg tcaccaaagg caatgacgtc tttttgatcg
     2581 atagacaggt agttagcaat ctcttgcacg ccttttgctt tttggattcc ttttgaaaca
     2641 atctctaaga tggcagtagg tccaccccat gtccgaacat cgacataact gccaaattga
     2701 cgtgttaatt cagcagatac agctcctgca aaacgtttgt ctgttcggac aagcaaagat
     2761 gtaggatttg tcgtcaaatt tttaggagaa agcaaattct tctctcctgg acgagaagaa
     2821 gcaaaaattt tttcatcaaa gaaatcaaaa ctgtcaataa aaaaagtatc acgattttca
     2881 gcagcgataa aatctaaatt tagctgcttt ttttgcgata agatttcaaa agcgatttca
     2941 cgattgatca acgtttctct ttcaccatcc cagtgctgat tagggatgtg tactaatgcg
     3001 ccgttaaaat taaccatcgg tgttgttaat tctaattgtt ggtaaaactg atggctcatt
     3061 cggaatgggc gtcctgtagc aatactgaca tgatgaccgg cattgattgc tttttttaga
     3121 gtctcttttg ttttagatgt aatcatcgaa tcttgattga gggtagttcc gtccagatcg
     3181 atggcaatta attttttatt caataagggt ccttccttcc tcaactagca tttgttttag
     3241 tctagcatat tttaggtaat taacaagagc aatcagctat tcctaacaaa aactacagtt
     3301 atggctatat aaatagctaa acataagcta aacacgaacg ttaagtatta ttttcgcttt
     3361 ttttgttaaa aaaacgttga aaagcgaatg aaacgtctat ataatttgaa attgttcgat
     3421 ggatttgccc tattttactt attttttatt attatagttt tccatcagaa aaaaattcag
     3481 ggagtggaac tttttatgga aaagtttttc ggtttaaaga aaaacaatac
//
LOCUS       DAYYNG010000214         3509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000214 DAYYNG010000000
VERSION     DAYYNG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.219"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(279..764)
                     /locus_tag="ACHBFA_002784"
     CDS             complement(279..764)
                     /locus_tag="ACHBFA_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM9396193.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    854..1199
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1244..1885
                     /locus_tag="ACHBFA_002785"
     CDS             1244..1885
                     /locus_tag="ACHBFA_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9396194.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1904..2539
                     /locus_tag="ACHBFA_002786"
     CDS             1904..2539
                     /locus_tag="ACHBFA_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9396195.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2557..3372
                     /locus_tag="ACHBFA_002787"
     CDS             2557..3372
                     /locus_tag="ACHBFA_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9396196.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1148 a          571 c          746 g         1044 t
ORIGIN      
        1 agtttagtta gaatataata ttttttgtta aatatgactg aaatcaaaag aataaaattc
       61 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
      121 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      181 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      241 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      301 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      361 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      421 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      481 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      541 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      601 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      661 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      721 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      781 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      841 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      901 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      961 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
     1021 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1081 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1141 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1201 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1261 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1321 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1381 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1441 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1501 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1561 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1621 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1681 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1741 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1801 cgtggcaatc atctatctta cattgactgg tattctaaca atcgtaatga aacaagttga
     1861 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1921 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1981 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     2041 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2101 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2161 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2221 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2281 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2341 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2401 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2461 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2521 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2581 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2641 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2701 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2761 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2821 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2881 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2941 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     3001 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3061 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3121 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3181 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3241 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3301 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3361 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3421 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3481 cttggtaaac ggtgttcaaa agccagttt
//
LOCUS       DAYYNG010000215         3470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000215 DAYYNG010000000
VERSION     DAYYNG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.220"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHBFA_002788"
     CDS             complement(<1..2232)
                     /locus_tag="ACHBFA_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9396197.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHBFA_002789"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHBFA_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          973 a          768 c          549 g         1180 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt
//
LOCUS       DAYYNG010000216         3381 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000216 DAYYNG010000000
VERSION     DAYYNG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.221"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..2051
                     /locus_tag="ACHBFA_002790"
     CDS             123..2051
                     /locus_tag="ACHBFA_002790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9396198.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2189..2644
                     /locus_tag="ACHBFA_002791"
     CDS             2189..2644
                     /locus_tag="ACHBFA_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM9396199.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2724..2852
                     /locus_tag="ACHBFA_002792"
     CDS             2724..2852
                     /locus_tag="ACHBFA_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396200.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3114..>3381
                     /locus_tag="ACHBFA_002793"
     CDS             3114..>3381
                     /locus_tag="ACHBFA_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396201.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         1036 a          604 c          663 g         1078 t
ORIGIN      
        1 aataaaaaca acatcaagct taatcaccaa atgataacca ttatcgttct catttagctg
       61 ttattattat caattacatt tttattatgc tatccttgtt ttgacaagga ggtctaataa
      121 cgatgactaa tcagaaaaaa gcttttgcag caacaatcgg ctgcctttta tttatgatct
      181 tcggttttat cttcgaccga ttctctctta ccatcacccc acttttttat atcagtgcca
      241 ttatcatcgg tggatataaa caaacatcgg aaggcttgaa agaattgtgg aatgatcgga
      301 cactgaatgt ggatttattg atggctttag ctgctattgg tgcctgtttg atcggtaatt
      361 actttgaagg agcaatgttg acattcatct tttgtttaag cggtgcatta gaggaataca
      421 caactaataa aagtcaaaaa gaaattactg ctttgatgaa tcttcaacca caaaaagccc
      481 aattattaaa agaaaatgga caaatgcaag aagtagatgt ttctcaacta aaggtggata
      541 acttggtatt tgtcgctaaa ggagcagctg ttccgataga tggcttttta gaaagcacac
      601 aggcaacgat cgatgagtct gcattaagcg gtgaatctgt gcctgtagaa aaatatttag
      661 gcgatgagat gtatgcagga acgctgaatc aaggaaatcc catcacgatt cgagtaacaa
      721 aaacgagttc cgaaactgtc tttgctaaga ttatccaatt agtagaagaa gcgcagaata
      781 cgccaactca aacagcttca tttatcgaac gaatcgaaaa taattatgta aaattgattt
      841 tgcttgctgt tcctctaatg atccttctcc cccacttttt cctaggttgg agttgggatg
      901 aaagctttta tcgaggaatg gtattattag ttgtggcttc tccttgtgcg cttgtcgcat
      961 ctgctacacc agcttctcta gctgctcttt caaattctgc aaaaaatggg attttaatca
     1021 aaggcggtat ccatttagaa aagctttctg aattaaaagc gattgccttt gataaaacag
     1081 gaacgctcac taaaggaaaa ccagttgtaa ctgacagctt tttctttgat gaaaaggatt
     1141 tggcacaaca acttcttgtt gctatggaac aaaaaacgac gcatccactt gctcatgcga
     1201 ttttgcaata tttcgattgc acaatacctg aagaaatcca atacttacca atagaagaaa
     1261 ttactggttt tggattacaa actacctatt taggtgcgca atggaaagtt ggcaagcacg
     1321 catatgatcc tgagacgatg ataatcagca aagagattgc ggaaatgatc gaacgtttgg
     1381 aaaatcaagg caaaaccgtg atttatcttt ctaaagatca gcagttaatt gctgttttag
     1441 gtctgctaga tattccaaaa gcaaataccc aacaagtaat ttcctacttt aaatcccaaa
     1501 acatccatac aagtatgata accggcgacc attctggcac agcaaaggcc attgctgaac
     1561 aagtagggat tgaacattac tatgcaagct gtactccgga agaaaaaaca caactagtca
     1621 aaaaagagaa tgaactttac caagtaaatg cgatggttgg tgatggagtc aatgatgcgc
     1681 ctgctttagc agccgcttct ttaggtgtcg ctatggggca agggacagat gccgctatgg
     1741 atatcgcaga catcgtctta atgaaaagcg acttaaataa atttgtctac agtcatcggc
     1801 tatctttaaa aatgcggcgg atcatcaaac aaaatattat tttttcgatt cttgttattc
     1861 tcttgttgat tgcttctaac ttcctgcaat ttttaaatct acctcttgga gttgtaggac
     1921 acgaaggaag cacaatattg gttattctga atggcttgcg tttactaaga cctttacctg
     1981 atctttctga ttcttcaaaa aaatgtaagt cttgtccact atacaaagcg cggctgtctt
     2041 ctgtcaaata agcaaagtgt ttttttatga ttttgtttat tttatgaaaa aaacagctta
     2101 tgaataggtc ttgaaggatg aattcctact tttgtaactt tctaaaaaca tgttatgatt
     2161 gtttcgaaga aagtgaggaa tatatttcat ggaaagttgg ctttttttag gaattatttt
     2221 acttgtagca attttagcta aaaatcaaag tctgatgata gcggcagctt ttgttttgat
     2281 tatgaagctc ataccagcct cccgttcctt tttcccttgg cttcaaggca aaggaatcaa
     2341 tattggggtg acgatcatca cagcggctat tctaatccct atcgcaacag gagaaatcgg
     2401 tctacgtgat ctattagaag cctttaaatc ccctattggc tgggtagctg ttttttgcgg
     2461 tgggctagtg gctgtcttat cttccaaggg tgtaggattg attgcttctg atccacaaat
     2521 cactgttgca cttgttttcg gcacgatttt aggtgttgtc ttcttaagag gaatcgccgc
     2581 cggaccggtc attgcttctg gtatcactta ctgtatctta caagtatttg ctttaggaaa
     2641 atagaacaaa ttttgtctat tttaggagat tcttaacacc cccaatttac tgatccgtga
     2701 tatactgaca acgaggtgat acaatgttcg aaaaattaaa gtatttcata ggaacaatcg
     2761 gctggtttct tctagcggta cttttgattc tgggcttagc ttatctatta tttcctgatt
     2821 tgatacaaaa tgtgtttgat actgcttttt gattgaccta ttaaaacgat ataggggatg
     2881 ctcctgcgat gcaggggctt tttttaatca atgttttgta ataatttagg ccgtgccaat
     2941 gagacttcct atagaattcg atgctctttt ttctttttgg ctcttctgct cttttttgtt
     3001 atactagatt acagtggaca aaagaaagaa tgatcgcatc cacttcaacg aatatcaaaa
     3061 tggaagaatt agtgcctgtt ttcaggttac tttgaaaaga aaggaagacg cttgtggaaa
     3121 cttttgaagg tcttttaatt gatacgtacg ttttacaaga acagccaatg atgctgactg
     3181 ggatcaactt gcagcttgag gaccgtgtga tcaatggcct gcttaaagat cgctatttgg
     3241 caaaatatat cttacgtttg cccctaaata aatatgtgct ggaagtaagt ggcaaatgga
     3301 accgtcggaa ccagttggtc ataaaaaaaa tgttgataaa aaatatcgat gaatacatta
     3361 aaataatcgg tacaccagaa t
//
LOCUS       DAYYNG010000217         3342 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000217 DAYYNG010000000
VERSION     DAYYNG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.222"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2206
                     /locus_tag="ACHBFA_002794"
     CDS             <1..2206
                     /locus_tag="ACHBFA_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM9396202.1"
                     /translation="TNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGI
                     NPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSK
                     IRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTS
                     KTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTE
                     IADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGK
                     DERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIP
                     LTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYA
                     SFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNEL
                     SNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIIS
                     SPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTIND
                     SVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYT
                     LAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDIL
                     GVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2286..2681
                     /locus_tag="ACHBFA_002795"
     CDS             2286..2681
                     /locus_tag="ACHBFA_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396203.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3006..>3342
                     /locus_tag="ACHBFA_002796"
     CDS             3006..>3342
                     /locus_tag="ACHBFA_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9396204.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLET"
BASE COUNT         1176 a          418 c          661 g         1087 t
ORIGIN      
        1 tactaatcta gtagctaatt ttggacctac cactgaaaaa atgtacgata atcaggttac
       61 tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt cttattcaag gtgccttttg
      121 gtgtaagggg attaatccag gtggcttcga tggggtatat actccatatt tagatactgc
      181 tgtaaaagag ttgcagactg atgcagggtt taatggaagc gctgtaactg gagttttgga
      241 tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt gttcttgttc ccggcggaga
      301 tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat tatttagaat acacgggaat
      361 aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca
      421 agctgaacta ggatattctc ccagtgaagc aacagggtct tatggtaatg ggacaactag
      481 taaaacttat cctgtgtctg aaggtaactc gggaaactat gtaagaataa ttcaatatgg
      541 attatatgtt aatggttatt ttgaagacgg tacattcgat ggtattttca ctaaatatat
      601 gggtttagaa gtactggcat tccgaaaatt tatggtattg cctgaatata ctgaaatagc
      661 agatgttgtt gtaataaaag gactattgtc tagcgctgga gatattagaa gatctgcaga
      721 tggcgcagat acttctacgc aattaactag atctcaaatc caaacattgg ttgataatga
      781 tataaaaata gttggtagat atcttactgg tactgttgga attggaaaag atgaacgaaa
      841 taagtatcta actacagagg aattgaataa tattttttct aaaaatttat ctgtgtttcc
      901 tatctatcaa gatggaggtg cagcattagc ttattttact tatgatcgtg gcttatcaga
      961 tggtaagaag gctatagatg cagcaaaaaa tctaaatata ccattaacaa cagttattta
     1021 ttttgctgtt gatctagaca tgttagggga agaaattctt gcatatgctg gagaatattt
     1081 taagggggta tctgctgtaa tgtcatatag cgggtatcaa acgggtgttt acggaacaag
     1141 aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg tttgcatttg tttcagatat
     1201 gtcgactggt tatagtggga atttaggttt tcctatgcct agacaatggt cttttgatca
     1261 atttgttgaa tttaccatag gaagtggcaa cggagctgtt ggtatcgatt taatcgctac
     1321 atcgggtaga gatagcggat ttaatgagtt atcaaatgat actaataatg ataattacat
     1381 tgccaaatac aatcagaagg ttataaatca gatggtacgt gcgtatcaat atctatcaca
     1441 agctggtctt gataatcctt ggaaccctca tttgacattt tatagatatg taaattattc
     1501 tggtttgtca tgggacatca tttcttctcc tgtaacagag catgatagaa aaatttatga
     1561 tgaatatagg aataaattaa ctaatagcga gggcctgtat aattatttta tagatcctaa
     1621 tactggttca ataataggtt tgcctcactt aattgtgacg ttacaatcac agatgtttct
     1681 agccgacaca atcaatgata gtgtttctga ctttgcaggt tggttaggag atttaatgac
     1741 ttgctggggt gaagttaaga aattaggaat acaaatggag caaggtgtat ttgcattagt
     1801 aggtacggct gcaggtggaa catttagtct agaagattta taccaagatg ttgacgcggt
     1861 aaatgtttat accttagcaa aaagaaattc tgattcaaca gctttatcgg ctttaaaaac
     1921 gtactattgt gataagggtg ctattgatag atttaatcaa tttgttaaga acagatttgg
     1981 ttcttcagat aaaatcgctt cagtaacaaa tagtttatta gaaaatgacg aaacagttga
     2041 tatcttaggt gtagggataa tttcattttt cattgataaa atagttaata attcacttgg
     2101 gacagatttt acttatcttg attttattgg agatgagggg aaagttgcaa atggattttc
     2161 tgaaaaaatt attcaatttc aagaaaatag aagtaaactt aattaagaat cgtttactat
     2221 ttaaacagtg tccttttcct ttttaaatat atacgataaa ataagtatat gaattgtaaa
     2281 aggacatgat gattatggaa gaaatagaat ggaataatct gactcaaaat aataaaaata
     2341 attctggatg tggaattatc tttatgtttg ttatagtttt gggaatagta gtacttttaa
     2401 taactgtcaa gatagtagat tactttgcga atactcctga agaacaaata cagagaaaac
     2461 ttcctgagtt tgaaagagta ccaccttatg atccaagaga taattcattt caagtaaaaa
     2521 aattaaaaga gtctccaaat gaaatgtggt ttgaagttac agttgaatca agcgacttca
     2581 aagaaaaaaa gaaagaatac aattatattg taaaggcaaa agtatcaaaa agatttattt
     2641 ttaaccctaa attagaaata tatagtgttg taatagaata atagtgttaa aaaaattgta
     2701 aaagttaaag aagtcaaatt ctgatgtgaa aaataaaata taccatggaa gacataaaag
     2761 aaaaactgga taactagttt atttaatggc agaccgttaa ggcctgccat ttttatgtat
     2821 tttagcggaa taaatataat catgcgggag tgtaaactta ctgtttcaaa aagtatactt
     2881 tttgacatgc cttgattttc tttgtttatg tatggcataa taaccacata atacaatttg
     2941 aaacgtttct gatttaatct gattgttttg aaacaatttt agtgaaaaaa gggtagataa
     3001 aaacgatgag aaaatttttt tatatccggg tcagtagtta tgatcaatcg atcgaacgac
     3061 aactgatttc cgcgaaggaa ttaggaatcc ctgaagaata tatatttgta gaaaaagcca
     3121 gcggcaaaga ttttaaacgc cctgaatatc aattaatgaa acggatgttt cgaaaaggag
     3181 atttactcta tctacaatca ttagatagac taggcagaaa taaacaaatg attttagaag
     3241 aatggcgaga attaactaag gaaaagcaga ttgatatttc tgtgttagac atgcctctgt
     3301 tagatactgc tagttacaag caactagatg ggttagagac gc
//
LOCUS       DAYYNG010000218         3338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000218 DAYYNG010000000
VERSION     DAYYNG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.223"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..212
                     /locus_tag="ACHBFA_002797"
     CDS             <1..212
                     /locus_tag="ACHBFA_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM9396205.1"
                     /translation="ICTGVLAMIPICLITKMMGKKLIYIESFAKVTSPTQTGKLMYHF
                     ADQFYVQWETMLQFYPNAIYLGGIY"
     gene            229..708
                     /gene="pssE"
                     /locus_tag="ACHBFA_002798"
     CDS             229..708
                     /gene="pssE"
                     /locus_tag="ACHBFA_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFM9396206.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            728..1795
                     /locus_tag="ACHBFA_002799"
     CDS             728..1795
                     /locus_tag="ACHBFA_002799"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9396207.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            2363..>3338
                     /locus_tag="ACHBFA_002800"
     CDS             2363..>3338
                     /locus_tag="ACHBFA_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9396208.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRI"
BASE COUNT         1252 a          359 c          645 g         1082 t
ORIGIN      
        1 tgatttgtac aggtgtttta gctatgatcc ctatctgctt aataacaaaa atgatgggaa
       61 aaaaactgat ttatattgaa tcgtttgcaa aagtaaccag ccctactcag acaggaaaat
      121 tgatgtatca ttttgcagat caattttatg ttcagtggga aactatgcta caattttatc
      181 ctaatgctat ttatttgggt gggatatatt aaataatagg agaaaaaaat gatatttata
      241 acattaggtt cacaaaaatt tcaatttgat agattgttaa aagctattga tgatttaatt
      301 gaaaaaggag taataatcga aagtacattt gcacagagtg gttatagtga ttataaacca
      361 ctaaattatt cctataaagc atttctaaat agggatgagt ttactgaaac tatgcaaaaa
      421 gcagaaatgg tgattactca tggaggtaca ggtgctataa ttggggccgt aaaaaaaggt
      481 aaaagggtca ttgctgttcc tcgattggct aagtatggtg agcatgtcga tgatcaccaa
      541 ctgcagttaa ttagacaatt tgatgagatg aatattatct atccttgtta tgaattagaa
      601 aatttagaag atgccgttaa agaaacaaga gttcggatgt ttaacaagta tgtatctaac
      661 acggaaacga ttattaattc gatagaggta ttaatagaaa aaatataaat ataaggagta
      721 aagcgttatg caaagagtac ttatgattgg taatgacagc tcggtaaaag gtggaattac
      781 aagtataatt acacaaatgc tcaattttaa ttgggaaaaa aagaatatcg agatgacttt
      841 tataccgagt tataagggtg gaaatgcaat atttaaatct ttatatttta tgtatgcatt
      901 tgctaaaatt ataggtatct ctttattgag taaacctaat attattcata tgcacatgtc
      961 gtacagaggg agttttagta ggaaatatgt gctgaataag tggtttagat tattaaatat
     1021 tcctgtaatc atccatcttc atggatcgga atttaaaaaa tggtatgata gtgtttctgt
     1081 aaaaaaacaa aatcagattc gcttgctgtt aagagagtct gattccacga ttgtacttgg
     1141 agaaaaatgg aagaagataa ttaaagaaat tgagccaaat tgtaaaactg ttgttgtaga
     1201 aaattctatt gtagttccag aatataccat aaagtggaat tcaaaaatgt gccgatttct
     1261 gtttatggga gttttgataa agcgaaaagg tgtacacgat ttggtagaag cagttaaaaa
     1321 cattgcagaa caatatccag aaaaaaagtt tatttttgag attgctggag caggtgatga
     1381 agaaatatcg ctcaaagaat tgatagaaga atataatttg caatcgtatt ttgaatttac
     1441 aggttgggtt gtgggagaaa aaaaggagga gtgctataga agatcaaatg tgatgatttt
     1501 accgtcctat aacgagggat tgcctatatc tattctagag gcgatttctt atggaatgcc
     1561 tgtaattgct acagatgtag gagatatttc ttcagcagta cgtgatggag aaaatggata
     1621 tttgtttcaa cctggtgacg tatcagcgtt gacatctgca atactaaaaa tcaatgataa
     1681 agataaatac gtaaaatttt cacgttgttc gaaggatttg gcaataacga aatttaacga
     1741 tggaaatttc tttgatagaa ttttaaatct gtatcaaaaa gggagcaagg agtgatgaca
     1801 aggaaaataa aaaatgtttc gattatgtaa tgaacttatt aagtgaataa aatagagaga
     1861 aaaaatattg aaatagacga tgtaaatttt tatccgtgta agaattcaaa tgaatagtag
     1921 tgttttagtg cttgtaatag agcaagagta caagcacggt acattatata aaaacattaa
     1981 tgatatttct ataaataaat agattttgta ttctggatga tgaagaggaa gatattatct
     2041 gataacttac cctaaacttt gcagggggtg gttataagtt gtgtatatgt ggcagtaatg
     2101 aatgagtgaa ataatcatat taagaaataa aattgcaata ttgtgtaata caaaagtata
     2161 ttttcattat tccaagggaa agcatactta cgaggatcat attattgatt taataaataa
     2221 tttaaaggaa ataatttttc tagaagaaga tagtatgtca ttcacatcat caagatcaga
     2281 gtctattttt ttcagagaat ttaagaaaat cgttgatgcc gtcattgact aattgagaaa
     2341 acgtgagtga aaggatagaa ggatgaattc gagtgatcta attagtgtaa tcatacctgt
     2401 atataatgtt gaaaagtatg taggaagttg tttggaatca gtgtgtaatc aaacatataa
     2461 gaacctagat attataattg tggatgatgg atctaaggat tcatctaata aaatatgtga
     2521 agactatgca ttaaaggatt caaggattcg tattattcat aaaaataatg ctggcttggg
     2581 attggcaaga aatacaggac ttgaacatgt aaaaggaaaa tatgttgctt ttattgattc
     2641 tgatgatttt gttgcagcta attatattga agcaatgtta aatggtataa aaaggagtaa
     2701 tgcagacaca tgttatgctg gatattatga atattatgat gataataata ttatttcaaa
     2761 gccagcaaga tatgatagta cgatatttac aaataacgct ataattgaaa aagtgttgct
     2821 tggaatgatt ggcactgagc cgagttataa agtcgatttt atattgccaa tgtcagtttg
     2881 gcatgcgctg tattcatctg agataattca acagcacaat atcaagtttc cgtcagaaag
     2941 ggattatata tctgaagata tcatattcca tatttcatat ttgcaacatt caaaaataac
     3001 aagttttatt aaagattgta tctattatta tagatgtaat aataataatt cattgacaca
     3061 taattttaga gataatgagt tcgattgtaa tattgaatta gttaaaaaaa ttgaaggcga
     3121 tttaagctat atattagaaa atgagatatt tgtaaacaaa acaaggagac ttttgttggg
     3181 acgctgtcgt acttgtattc aaaaggcagt tagatatgct agttttaata aagaatttga
     3241 tttaaactcc tatttgtcta agatacttat gaacgatgag ttgattgcct gtattcactc
     3301 atacccatat aaacaaaatc caattaaaca acgaatat
//
LOCUS       DAYYNG010000219         3301 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000219 DAYYNG010000000
VERSION     DAYYNG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.224"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACHBFA_002801"
     CDS             <1..189
                     /locus_tag="ACHBFA_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9396209.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1054..1584)
                     /locus_tag="ACHBFA_002802"
     CDS             complement(1054..1584)
                     /locus_tag="ACHBFA_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFM9396210.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1581..2198)
                     /locus_tag="ACHBFA_002803"
     CDS             complement(1581..2198)
                     /locus_tag="ACHBFA_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9396211.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2780..3001)
                     /locus_tag="ACHBFA_002804"
     CDS             complement(2780..3001)
                     /locus_tag="ACHBFA_002804"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9396212.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1083 a          646 c          352 g         1220 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagcccccct ataatctcat tattattttc atgacttttt actaccgtgg aattacaagc
     1021 aacaacaata ttatctgcta aatgaacacc tttctatatc acgctttttg ctccaatcca
     1081 acaatgttta cctataacta ttttattatt tttattcaaa atcctatgag tattcaattc
     1141 aaaaatcgta tgtgaatcgg tatccataaa taatgtatcc cacgatactg tacagccatc
     1201 tttgaattga atttccttaa agcaaattat actcattttt cctgtcgaat taaactttga
     1261 cccaatatct aaaactgcat ttttccttat gtcaatatga gccccttgtg cgataaacgc
     1321 tggcccttgt attcttagta ttccttcaat acttataatc gtttcttcat ttcccgcagt
     1381 atttaatact ggaaagccca aataaactat ttttccagat tgtaatacta tctcaatttt
     1441 ccctctcgat ttgcgtgtaa tccgtacttt acgatgcacc aaaataggta atctccacgc
     1501 atctgaaata tgaagaaatt tataattgaa gtatatcgtt ttaggtaaat ttaatatatt
     1561 ctttattatc aatccgagtc tcatctttca ccatctcttt ttgtaatcaa aattagttta
     1621 taaaggatta cacccaatat accgcccaac gtattatata caaaatccga aatttgaaac
     1681 gttcctagcc tcaaaaaaag ttgtaaaaat tcaattattg ctgaaaaata aaatgcaatt
     1741 tttcctccat atactattga ggaccatatt ttgggtttct ttatattttc atatttaata
     1801 gacattacca tataaacata tggaataaaa agaataatat tttctaaaca cgctgtagat
     1861 aactcttttg ttccagttgt tgtattatac ttataaatcc accagtctcc cataacatca
     1921 gataaaggat ttaaccaaat tgttcgattg attaatgttg taaataaaat catcgcagta
     1981 tagaatatca aagcacaaac ttttctatat tttctatcct tctttaaatg aagataaagt
     2041 tccattagta tacgtttata gccctcacct gcagcaatgc catctgttgc atacataaaa
     2101 tagaccagtc ctaaaattgt caataaaaca gcaaacccaa agggttcata taatttatat
     2161 aatatatctg taataatttc tgcaatcact attatcataa ttatttctcc aatatcaagt
     2221 atacacttat cgcttccaat tacacatgat catattagag tgtattatca cattctctaa
     2281 tattcaattt atttttccta aaatattcga aataatcgtc ttgataacga gtaagtattt
     2341 ttatacgatt gataacatag taacaatatt tttaattata gtaaaaaatc atctgccttt
     2401 ttcatccacc taatcttatt tttcatttta ttcaacatcc ttaaataaac ttccgttcct
     2461 aagctctata atatcttctt aaaaaataat attatggtgt cgttttcaaa ctcgtaatct
     2521 tttacattgg aaaaatacat acttttctca atgtagcaac tcctatctca cagtattcaa
     2581 aaattcttca atattatgaa atacttgtgc atgttcttta aataccttgt cagtctgttt
     2641 ataatcccct actaaatttt gcattgctcc caactcttcg acaaattgtc atctccatct
     2701 gtaaacagcc ctaaaaactc atataatgct ttttcatcaa tatttttccc atattattct
     2761 cggtatagtt tcacaagttt tacggattct tcatattgtt tatcttttta taatattcat
     2821 ctagggacat aattactttt gctagatttc ctgcaacaac acaatcgtct gatacgtcct
     2881 tgtttactaa actatttgca cctataacta cattatttcc tatatgtaca ccctttagta
     2941 ttctagactt cattcctata aaaacattat tacctatttt gatcctctcc gatgatccca
     3001 aagcttctcc atactacttt taaaaccgat caatcataaa catgagtcaa aattgtaact
     3061 ccttcatatc atctccaatt tcaacaagag tcttcgttgg aacaaaaact atacatcgtt
     3121 cgcctattcg gcagcctaaa aattgtagtt actttatata tcattctgat gtctcttctc
     3181 atctttttac caataccgac gtaagaactt tcaaaatata cttaaacttc ttattacgga
     3241 tattaaacag aagaataact aaaatcgata tgacaaccga tataactaaa tttatcacta
     3301 a
//
LOCUS       DAYYNG010000220         3266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000220 DAYYNG010000000
VERSION     DAYYNG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.226"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(486..1100)
                     /locus_tag="ACHBFA_002805"
     CDS             complement(486..1100)
                     /locus_tag="ACHBFA_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9396213.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1706..2083)
                     /locus_tag="ACHBFA_002806"
     CDS             complement(1706..2083)
                     /locus_tag="ACHBFA_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396214.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2383..3264)
                     /gene="galU"
                     /locus_tag="ACHBFA_002807"
     CDS             complement(2383..3264)
                     /gene="galU"
                     /locus_tag="ACHBFA_002807"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9396215.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1001 a          667 c          498 g         1100 t
ORIGIN      
        1 aaaaaaataa taaacactta taaatatcca ttatctatcc ttcaattttt gtgtatttta
       61 tttctattag aaaaaggtat cctgttggac aattttgtta tatcaggtaa acttcattgc
      121 aataatgata acaaatgtac tataattact ttgtaaagag ttttggggaa gactctttca
      181 tcaacgtgta ttaagagaca agaaagaatc catatggatt ctttcttgtc tctttttcca
      241 ctacttttta tttttctgac actacatatt cttataactc atttctcata actgtgaaac
      301 aacttataaa aataacaaac tttaaacaaa acaagcttaa aatcaaagat gatccatctt
      361 aaaataacta aaacaaaaaa agtagtcgtt gttaatcctc attaataatc gttttctttt
      421 tttcaaacgc tccattttta tgtggaacat tattttttta aggaactttg tcaacatgtg
      481 gataactatt ttccacttgt gtcctggact agctgttttg ctgtaaaata caaaacttgc
      541 tgcagcaaaa agatcgttgc aagagaggct gttccgcaga tcgctggatc tagatttggc
      601 aaagtcaaga ctgcatcatg taaattggcg tggttgtcca aaaccaaatc agcaaattca
      661 aataatcgca aaccactgct atgttgcgat ttgatacttc gtgacaaatc aaatccagta
      721 acaatgatta atggatgacc attttctttg atccactgag ctagttcgat gattgccggg
      781 tttcttcctt cattcgaaag aagaagaaaa atgtcttctg atttgacaga aagctgatca
      841 ataattgcct gtcctgcacc ttctagcgtt tcatagacac ctccggctgg attttcaatc
      901 aatatacttg gtacaagatc cggggtttga tcccaaaatt caaatgctac accacttagt
      961 gtgcggctag catatatctg caatctacca ccttgattaa tttgtttcgc gatccaagtg
     1021 ccagcttgaa gcatcagcgg ctcttctatc cttaatttct cttccaattg gtgataatag
     1081 tcaaaataac ataaactcat ttatctctcc cgcctagcta tacttgacat gaatagaaac
     1141 agctgcctaa tctaaggaag tacataaaaa aacagtagtt agacagtaac acatgtggtc
     1201 tttgctgttt caaacacaaa aaaggcattg aacgaatacg cccaatacct tgacatagca
     1261 aataaaacaa cgtgcagacc atggaaaata tctacatagg ggtagaattt tcaccctaga
     1321 ttaggtacaa taaattaggt cgatgcactg cattgactat ggcaaaatga tcggtcaggg
     1381 tgtatgtgtg tgactggtca ttccctgatt cttagcgcca actaagggtc agggagcctt
     1441 tttttattca gctgtttcta tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc
     1501 aagaaaatac aaaaaaacat tggtatgatt tatatatata taaataacat aaaaaaagga
     1561 atggcttatc ccgatacaac aagcattcct ttaaaaaaga ttcttttata taagtgaaaa
     1621 aagctttttt ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc
     1681 tttttttgcg tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga
     1741 gagaatctgt actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc
     1801 ttttcatata tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc
     1861 aagcttccca actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg
     1921 cataccagat aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata
     1981 gactaataag caatagtggc aacacaaata tccaattgct tccttccatc agccacttga
     2041 gtatatcaag cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt
     2101 ttttataatt aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat
     2161 ttctcttatg taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa
     2221 tattttctga atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg
     2281 atctagagtc aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa
     2341 taacaaatag cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag
     2401 ctttttactc aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag
     2461 tccatattca atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc
     2521 tttaaactct ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag
     2581 ctgtatctcg tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt
     2641 cagtagataa cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc
     2701 tacaaaccct tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc
     2761 gtatttagat gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc
     2821 ttcataatcc atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc
     2881 cagcatgacg acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc
     2941 cagtcctctt ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac
     3001 taaagcgagt aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc
     3061 atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat
     3121 tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag
     3181 catttctttt gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg
     3241 aatgactgct tttttgacct tcactt
//
LOCUS       DAYYNG010000221         3226 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000221 DAYYNG010000000
VERSION     DAYYNG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.227"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..446
                     /locus_tag="ACHBFA_002808"
     CDS             210..446
                     /locus_tag="ACHBFA_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396216.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            453..2405
                     /locus_tag="ACHBFA_002809"
     CDS             453..2405
                     /locus_tag="ACHBFA_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9396217.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="ACHBFA_002810"
     CDS             complement(2477..2974)
                     /gene="dfrG"
                     /locus_tag="ACHBFA_002810"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9396218.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1137 a          530 c          559 g         1000 t
ORIGIN      
        1 gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat
       61 tgttaaatca acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa
      121 aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat
      181 ttactaagca aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg
      241 ctaagtctgt ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg
      301 aatatgcaga gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg
      361 ataagaagtt tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa
      421 tgggtatcaa agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga
      481 aggaagatga aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg
      541 ctaaacaata tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt
      601 tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca
      661 tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga
      721 aaccaagtcg atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact
      781 gcaatgacaa tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta
      841 attggtctgt tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg
      901 agccagatta tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg
      961 agccgaagca attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta
     1021 aggcgtgtaa gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc
     1081 aacaaaagaa caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca
     1141 gtcgtacttg tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc
     1201 tgtataggcg ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga
     1261 aatttaacga gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta
     1321 agaaaggaca aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata
     1381 tagttgtatc tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt
     1441 ggaatatttc aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca
     1501 atacttttag tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg
     1561 ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga
     1621 gtagatttgt cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt
     1681 taattaaaca aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta
     1741 ttagaaacgc cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt
     1801 ttatttgcca agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag
     1861 caaaagcaga actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga
     1921 ctttggcatc tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta
     1981 ttagtaaaga tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag
     2041 ccactaagga taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac
     2101 caaacgaaat cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc
     2161 aacagataag acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata
     2221 agaagagcta tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc
     2281 taagaactta ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac
     2341 ctgctcaaag attagggatt acagataaac aatttgattg gaaagatata atttatttca
     2401 aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct
     2461 tcaattaacg cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt
     2521 tatacggatt tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat
     2581 aattcacttc tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca
     2641 ttttctcaac ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt
     2701 cttcttcgtt tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat
     2761 taaaggtaaa ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc
     2821 cgattgattc aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa
     2881 catattccca gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat
     2941 tcttatccat cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt
     3001 taccatctaa tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg
     3061 aataagcaat tttttctata agttatatta tcataatacc acaaaatcac aaattttcat
     3121 tcaattatca ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt
     3181 taaaatgaaa aaagtagtcc attttgaagt acattttgtc attttg
//
LOCUS       DAYYNG010000222         3201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000222 DAYYNG010000000
VERSION     DAYYNG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.228"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..285)
                     /locus_tag="ACHBFA_002811"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACHBFA_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACHBFA_002812"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACHBFA_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="ACHBFA_002813"
     CDS             2091..3131
                     /locus_tag="ACHBFA_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9396219.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1187 a          447 c          461 g         1106 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta aaggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtgatat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa t
//
LOCUS       DAYYNG010000223         3191 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000223 DAYYNG010000000
VERSION     DAYYNG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.229"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..611)
                     /locus_tag="ACHBFA_002814"
     CDS             complement(243..611)
                     /locus_tag="ACHBFA_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM9396220.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            761..1243
                     /locus_tag="ACHBFA_002815"
     CDS             761..1243
                     /locus_tag="ACHBFA_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM9396221.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1280..2290
                     /locus_tag="ACHBFA_002816"
     CDS             1280..2290
                     /locus_tag="ACHBFA_002816"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9396222.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2405..3118)
                     /locus_tag="ACHBFA_002817"
     CDS             complement(2405..3118)
                     /locus_tag="ACHBFA_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396223.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1080 a          513 c          620 g          978 t
ORIGIN      
        1 attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactaagta
       61 gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa
      121 atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa
      181 caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag
      241 ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt
      301 tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat
      361 acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg
      421 atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac
      481 ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca
      541 aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga
      601 aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa
      661 tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga
      721 taagatgtct caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa
      781 attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga
      841 agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt
      901 cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga
      961 tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat
     1021 ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat
     1081 tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag
     1141 accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga
     1201 gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca
     1261 tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg
     1321 ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt
     1381 aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga
     1441 taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga
     1501 tgaaaatatc caaatgatct ttccgccttg gggaggagaa ttattgattg agattctcga
     1561 ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac
     1621 gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa ccaaccttat
     1681 tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac
     1741 aagagaagga gaatcgattc tacagcaacc ttctgaaaag tatcaaaaag agtggcccca
     1801 tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag
     1861 cggagatgca gtaaggatga agggacgatt attaggtggc tgtattgata cgatcagtca
     1921 tttaatcggt accccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa
     1981 gccgatcttg tggtttctag aaaattgtga gctatcgtca gttgatttaa gaagatcact
     2041 tgttcagatg aaactggcag gatggtttga tggcagctcg gggatcatgt tcggtagaag
     2101 tgcagcagag gatttgttag attataaaat gaaggatgta tatgaagatc tgtcaaagga
     2161 attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt
     2221 gatcaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac
     2281 gtttatttaa aaaatttttt cctgccttgg agtttatcaa agaccaacaa atctaaatag
     2341 aaaagaaaat gaaagaagct gacagtattt tgccagcttc tttttgtatg atctattaga
     2401 aattttaatt atccataact atcacattga tcagctgttt cacatcattt ggattacctg
     2461 taggaatagc tactacagat gcattatcaa taatttcttg agttgtttct ctaatgaacg
     2521 ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga
     2581 ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga
     2641 gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc
     2701 ctgcaggata atgaataaca tgtagttcga aatcacccga tgtatctcta aaagtattac
     2761 ctctaattgc tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg
     2821 gatctattga ctgagaagac tgtgaggaag ttgtgtctat tgttgaagaa gaaatatttg
     2881 tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg
     2941 aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg
     3001 tttgtttgat tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag
     3061 acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat
     3121 aaaatacctc attatttttt taataccagt atagcataat cctgaatttt tgaatccgta
     3181 aaaccttgtc t
//
LOCUS       DAYYNG010000224         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000224 DAYYNG010000000
VERSION     DAYYNG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.230"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            346..2406
                     /locus_tag="ACHBFA_002818"
     CDS             346..2406
                     /locus_tag="ACHBFA_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM9396224.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2489..3058
                     /locus_tag="ACHBFA_002819"
     CDS             2489..3058
                     /locus_tag="ACHBFA_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9396225.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1149 a          556 c          593 g          864 t
ORIGIN      
        1 gactgggaat attttggaaa taccatcatc agagaaaaaa atagacattt aactgaaaaa
       61 atcagctagt atttatggat ggcagctttg tctcacatat ttattagctg atttatgttt
      121 ttttcaaaga aaaggtaact agatataact agatataact agatatatta cttaacaaaa
      181 ttaaaaagta cataagttat tattccgatt ttcttgaaac aataaaaaat caaaaaaaat
      241 actctcctaa tagtccatct tcttttgatt ttatttatat aatgaaatca tcttatgaaa
      301 attactactt catgtttaga caagagtagg gaggactaag aataaatgtg ttcaaaaaga
      361 cgttcaaaac aactaaataa acacgcttgt cgtttagata aggaacggtc gtgttctgaa
      421 aaacactacg ttaagtttat gatgcgcacg atgctgatca gtttatctgt aggtgtctat
      481 atgcatgtac ccgttcaggc aacgacacaa accatcaagg tcgtacgtac ctctgtttct
      541 gagaccactg attcccctat cactattact catactttca ctgatgcaaa tggaaaacag
      601 ctacttcctg cattgacttc ctataataaa gtcagttatc ctccaacgat aaaccactac
      661 tggttaattc ctaacaaaac attatggaca tcggaaaccg gaagcagtgt gccgctttct
      721 atcttttccg atatgactga tcctacaaaa ggtgatcagt taaaacagat cactgatatt
      781 ttgaatcatg ctttgacaga tataaaggac ggtggaagcg cacaagtttc ttatgtatat
      841 gaaaaagatt attcacagtt tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt
      901 tcgatatctg atctggtaga aaggctgaag aacgcagatg gaacacaagg agatatgtcc
      961 aaagtaattt caaacaatgg gaatatcgtg gatacgacta atacaggaac atctactatt
     1021 tccttgtcct attttgatcc aattgcttta aaaacaatga ctgcatctgc tgtgatccat
     1081 gttcgtgctg atcaaacagc tatcaaagga aaagatcttc acttaagaac aggagagagt
     1141 tacgccccta aagatcttgt tgaatatgta acaaaaagcg atggaacttc aggaaataaa
     1201 gaagaaatca aaatgaacgg gacagtagat actacaactc cgggagaata tcccgttgta
     1261 ttaacttata tagactcgaa aacatcaaat gtggcagaaa caacagctgt agtttatgta
     1321 gaagcatctg ctgcttcatc gactgtcatc actcatttta tagatagtca aacaaataaa
     1381 gaacttcaag cagaaacaca tatgagtagt gtaaatcgta ttcctgattt gcgcctacct
     1441 gctgaatttt ctgatcgagt gccaaatata gatttatcga cttatgaaca aaatgggaaa
     1501 gtcacttcta tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg
     1561 gagatccttg aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa
     1621 attaccttta cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt
     1681 gttcatccaa acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta
     1741 ggtaaacccg taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat
     1801 ggttatttga ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat
     1861 tcactccaag aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata
     1921 gatcaaaaag aacaaaaaag acaagaagaa agcttgtcag aaaaaaaaca acaaatggct
     1981 catgcagttt ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata
     2041 tctagaaaaa tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa
     2101 gtttctggat tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa
     2161 tttgttgcta aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa
     2221 aaacgcttgt caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct
     2281 agtccgggaa cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg
     2341 cgaggtatgg caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg
     2401 ttctgattta tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg
     2461 cataatggag acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat
     2521 ctggatgaca agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga
     2581 atttcgattt tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg
     2641 ggaatttcaa acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc
     2701 tgaagaactt gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac
     2761 ctgtaccgct tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc
     2821 agtgaaacca gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca
     2881 agaatttcct accctccata cggacggacc aatctatctt cacacacaga catggagcca
     2941 tcaggcgatt gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg
     3001 cacacctaat ccagattatg aaaaagttat gcaggtttta agtaaattag ataaataacg
     3061 aaatggtgaa ataagtcctc agtattggga taaaaaacga aatagtcatt cctcatatct
     3121 tacttttttg agttaatact atcggataac taatggcatg cc
//
LOCUS       DAYYNG010000225         3161 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000225 DAYYNG010000000
VERSION     DAYYNG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.231"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHBFA_002820"
     CDS             278..787
                     /locus_tag="ACHBFA_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9396226.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHBFA_002821"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHBFA_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9396227.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..>3161
                     /locus_tag="ACHBFA_002822"
     CDS             2322..>3161
                     /locus_tag="ACHBFA_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9396228.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYN"
BASE COUNT         1115 a          508 c          631 g          907 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa t
//
LOCUS       DAYYNG010000226         3148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000226 DAYYNG010000000
VERSION     DAYYNG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.232"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            203..790
                     /locus_tag="ACHBFA_002823"
     CDS             203..790
                     /locus_tag="ACHBFA_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396229.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            790..1689
                     /locus_tag="ACHBFA_002824"
     CDS             790..1689
                     /locus_tag="ACHBFA_002824"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFM9396230.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            1689..2603
                     /locus_tag="ACHBFA_002825"
     CDS             1689..2603
                     /locus_tag="ACHBFA_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396231.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            2655..2870
                     /locus_tag="ACHBFA_002826"
     CDS             2655..2870
                     /locus_tag="ACHBFA_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396232.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         1206 a          384 c          671 g          887 t
ORIGIN      
        1 atatcatctt aagagaagaa ttacgattgt ttataggatt tacaaaatga ataagtttga
       61 agagtgattt ggtcgcagac tagatcactc tttttgtata aaaaaacttt acacttattt
      121 ttgagtgtgt taagattttt tatatagagt gacggtttct tgtcagagtg aatgataacc
      181 atcaatattg gaggaataat aagtggaaaa caataatgat attagatatt ttagcattgg
      241 ggaaagagtt acaacagtaa gtgaattatc aaaggattat agagataaag aaggaacgat
      301 tatttctctt aaaaagaatc gaagaggtgt ttggcaatat gttgttgatt taggggatgg
      361 cgttacagtt gagtgtaaag atagtcaact gatcagaaat gatggcagtc attccattga
      421 aaaagcaatt cttgtattcc caaaagtatc aagcaagatt atgaatgact ggttgcttgg
      481 aaaatttggt gagtacaata aaaaagaaat cgcagaagtg atcggcaaat cttatccaac
      541 agtattaggg tatctaaaaa agataacgaa caaagaagcg aaagttgagg atgttttaac
      601 agtaagagaa cttcagtcat taatcgaata tcggaataaa caaataacaa atcctgagta
      661 ccttaaaaaa gaagatggtc tattgaaaca attggaacag gtaaataaaa tccggaaaat
      721 gcaaaaggta gctcctttaa gcctagataa ctttaaaaaa atctatttga aagagatcga
      781 aaaagactaa tgtggagtgt cactttaccg tttcaacctg tatctgcacc aagaccaagt
      841 gcaagaagga atgttgctga tgcaactaat gacgtagcag agaaaaggat ttcaacctat
      901 tatgataaaa agtatctcga ttatttgaaa aaaataaaaa gctttatcga agatcaaggt
      961 ttgttagatg atgaatttta ctcgatcgta aatgatccga tgggtgcaat tatggaagtg
     1021 gacttttact atcagatacc aaaaagctat aaaaaagtct ataacattat gaagacgaca
     1081 gcgccagatt tagataattt agtaaaaagt gctatggatg gtattttcaa catctcagct
     1141 atacgagata gccatatcac agggttaatt gcttttaaat acaatgtggc aaacgaagga
     1201 aaaacagttg tgaggatcaa gcgatattca gaatttgagt gggatacttc tgaaggattg
     1261 aacggagata tttgggaagc cacatttgat ttcaaaccag tagcaacgcc aagaccaaag
     1321 tctaaattta gaggaaatgg gattatcact tactatccaa aagagtataa cgactatttg
     1381 gaaaatatca agaatacgct taaagagaaa gatttggtaa atgatcaact gaaaaaggta
     1441 atgaatgctc ctatgggggt tatagctcag atagattatt tttaccaagt gagaaaagat
     1501 aagaaaaagc ttgattcact gatgcgtact tcacagccgg atattgataa cttagtcaaa
     1561 ggaacattgg acggaatttt taacaaggaa attggcatca aagatagcag ggtggtaggt
     1621 ttaatagctt ttaaatacaa tacttttgaa aagtcgaaaa caaacgtgcg actacaggag
     1681 atgggctgat gaataataac gaaaaaattc ttcatgtact tgatagcttt gaaacaattc
     1741 aagaggaact aaagaaatat cgagatgttt tggaacaaag atatgatttt gtcaatcagc
     1801 aaaagagtaa tcacatggat ttcattctca atatgaatga cttgaagaag aaattagttg
     1861 aacgaaaaga acaagaaaag ttgatcaaag cttactttga actcggtgaa aaagaagtta
     1921 aaaaggcaat ggaactgaat gaagatcgca gggttttaga tcagctgtta gaacaattat
     1981 tggtgatgtt tcaaaaagga cgaatagatg aggatttaat tgaagaaggg ttgagaaagt
     2041 atcctgcaaa ctcaggtatc ggaatagtgc tgaaagcgat tgatgaagaa gaaattgaag
     2101 ctttcatacc gccagaggat tttgaatcgg caatggaata tataaaatat tatagccaag
     2161 ggatcactgc ttttagagag tttgatcctg aagatgttat tcatgacttg aacaacctaa
     2221 aagaatggtg tgaaagttat ggagtcgatg attccggatt ggactacttg atcagtatta
     2281 tggaaatcga agaagagatg ccggataagc ctgatccaac agatatttta gaattgatcc
     2341 atgaagctag aaatccaatt gcctatattt ctagaggata tactgtattg gaatactata
     2401 agccttatat atcagcgatg aaccatttaa gacgtgtatt aagggaaaaa cgtgaatacc
     2461 gctcagtgtt gaatgcaacg aatagattag aaaaagctgt ttcagagcta gatgcttatt
     2521 atcgtgaaca ttacctgcaa gcaggtggta tgccaagaaa tacgaaagca aatatcagta
     2581 ggtatataaa aaaagctgag taggcggagt tagcccataa aatggcaaaa ggtaacctga
     2641 aaggggcatt ttgaatgaaa agggatgttt ttgaattgta cgtattagaa cgtctagaag
     2701 acggcatgat ggcacaagag ataattgatg aaattgatga tatgatcagc agatttggtc
     2761 aattggagtt gaatgatgga aacaaaaatt cggtagtgct aaaacaattt tggttgaaca
     2821 atttttcgta taaggatttt atcaaagaaa aggcaaatac attgtactga ctaaattcac
     2881 gaggagcagt gatgagagct tattgctaag agcagttagg ctcttttttg tttgtgtaaa
     2941 attatttgtt ttataataca catttataca caaaaaaata ttgacagata gtacacaaat
     3001 gtggtaggat atatttgtaa gcaagttaga ttaacaagtt aaaggaagag cagttatgaa
     3061 aaaagaagaa ctaatagata tatttcaaat tgttgaacga gcaaataata tgggaattat
     3121 gttttttgat cgtatttctt taaagatg
//
LOCUS       DAYYNG010000227         3057 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000227 DAYYNG010000000
VERSION     DAYYNG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.233"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..980)
                     /gene="murB"
                     /locus_tag="ACHBFA_002827"
     CDS             complement(45..980)
                     /gene="murB"
                     /locus_tag="ACHBFA_002827"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9396233.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1053..1973)
                     /locus_tag="ACHBFA_002828"
     CDS             complement(1053..1973)
                     /locus_tag="ACHBFA_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9396234.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2086..2838
                     /locus_tag="ACHBFA_002829"
     CDS             2086..2838
                     /locus_tag="ACHBFA_002829"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9396235.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          857 a          620 c          530 g         1050 t
ORIGIN      
        1 ggccaccgtc ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt
       61 ctgcttgttt ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac
      121 acatcaaatt tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc
      181 gctgtcgcat gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg
      241 ccccatttca ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact
      301 gggcgcttaa agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat
      361 tcggtcaact catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat
      421 cgagcttcca atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc
      481 atttcgtctt ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca
      541 aaaacatctt tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca
      601 cttcccggga tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa
      661 gtcgtatcaa tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc
      721 tgtttcattt cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga
      781 ttgcttgcat ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt
      841 tgctttactt cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc
      901 gtaaacgtat agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca
      961 actatttctt ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc
     1021 tatcacgctt aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta
     1081 agcacttcct cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt
     1141 tccgatttac ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt
     1201 ttgactagcg tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga
     1261 atgacaagct gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat
     1321 ttttctatat tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt
     1381 tctaattgta aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt
     1441 ctcaaaatgg gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca
     1501 taaaggacga actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac
     1561 cgcttatctg ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg
     1621 atcatcaaaa ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga
     1681 tggacactca gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct
     1741 aattcataat gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa
     1801 agctcatcga tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt
     1861 gtttctttat ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt
     1921 ggtaaatatg ctttttctgc gatattccct acaccgatta caccaatctt catctacttt
     1981 cctcctggat catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat
     2041 agtatcgcaa aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct
     2101 tggaacgtaa acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag
     2161 ttagacgccg attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta
     2221 gatcttccag gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca
     2281 gcgatttttg ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat
     2341 gatacagaag gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac
     2401 acgccaaatt ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc
     2461 ttttataatt atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta
     2521 aatgtcgctc atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga
     2581 ttcactccag aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca
     2641 ttccgttatt tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat
     2701 tcaaggaaaa acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag
     2761 ccaggactag ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta
     2821 gaattagatt tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa
     2881 attttgtgaa aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc
     2941 ttttgaacac tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac
     3001 agccaaacaa aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagat
//
LOCUS       DAYYNG010000228         3022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000228 DAYYNG010000000
VERSION     DAYYNG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.234"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHBFA_002830"
     CDS             complement(287..649)
                     /locus_tag="ACHBFA_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396236.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHBFA_002831"
     CDS             complement(723..1103)
                     /locus_tag="ACHBFA_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM9396237.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHBFA_002832"
     CDS             1185..2360
                     /locus_tag="ACHBFA_002832"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9396238.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2436..3020)
                     /locus_tag="ACHBFA_002833"
     CDS             complement(2436..3020)
                     /locus_tag="ACHBFA_002833"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9396239.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          577 c          498 g         1005 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat at
//
LOCUS       DAYYNG010000229         2948 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000229 DAYYNG010000000
VERSION     DAYYNG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.235"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..456
                     /locus_tag="ACHBFA_002834"
     CDS             175..456
                     /locus_tag="ACHBFA_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396240.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            458..874
                     /locus_tag="ACHBFA_002835"
     CDS             458..874
                     /locus_tag="ACHBFA_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396241.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(1211..1351)
                     /locus_tag="ACHBFA_002836"
     CDS             complement(1211..1351)
                     /locus_tag="ACHBFA_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFM9396242.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAV
                     VN"
     gene            complement(1348..2322)
                     /locus_tag="ACHBFA_002837"
     CDS             complement(1348..2322)
                     /locus_tag="ACHBFA_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9396243.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          994 a          603 c          438 g          913 t
ORIGIN      
        1 aaaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa tttgatatca
       61 ctttttcgcc atcgtggcta gcttatgtat agtattttag caaaaaacag ctatacaaat
      121 actatattcg tatgatatac ataaattata cacaaatgcg aaaggagttt ttctatggac
      181 attttagttc ggaagatcga ttcaaaggtt attgcaaaaa tagacaaacg ttgccaagaa
      241 ctcacaaata aaactggcaa aaaatggagt cgaaatcgct acttaaaagt cctaatcgaa
      301 aacgattttg accacgcact tatgcattac aagaaagatc aattcgattt attactcgaa
      361 aaatttatcg ctattcaaga aaaaaatact gaaacgctga atgaatatgt tcgtacaaac
      421 aaccaactca ttgaaacact tatcggagga agataacatg agaaaaactt ctgttcaaaa
      481 acacgttacc ctttatccca gtcacgtgga cgttttagat tccattatcg aaagaaatcc
      541 agatatcaac aattactcgg aagccattcg ttttttatgt atgagttatg aacctgatac
      601 caacacgact actaccagca atcaagccaa actaaatgct atgggaaaag aaatttccat
      661 catgtccgaa atcattgcag aatttgctga tttatatcta aaagatacag gtattcttat
      721 tggcacaaat tctaaagtgt atcaagaaac gaaagaaaaa attgaaaaac aacttaatcc
      781 ctccaaacga aatcaaccaa aaaatcgtca aaaagaaacc aatcaaacat ttacttctaa
      841 aaatttcgac ccaacaaatt ttctaaaaag ataaaaaata aaccaggaga tttaaattcc
      901 tggtttattt tttgggtagg atcaccatct taatagcaac atttacaata agatagtaac
      961 caattgaatg aaaaaaacga atttcagaag aaataacaga aaaaaagata aaaacaaaaa
     1021 aattataaat acatgatttg aattcataat cgtaaaaaaa gctgctcgta cgagcagcta
     1081 aataagaaaa agattggtat gttttaagga taaaataagt ataacaaatt tttcacatag
     1141 agcaaagggg gcgaaaatcg cccccttttt ttcgttttaa aaccccaaaa cccaaagaca
     1201 taaatttcgg tcaattgacc actgcatttt tttctttata ctgagcaagg tcaaatcccg
     1261 aattgaccag ttgattgcgc accgtatttt tcgatacacc caattcatca gcaagttttt
     1321 ttagggtcat tttttctatc gaatcactca ttcagcacct acttccgtca catcaagtcc
     1381 ttgaagccat gtatctaatt ctcgtttcaa atcatctgcc gactccgaaa atgtttctcg
     1441 caaatgattc gtcaactcat cattttgact ttcttgcttt aacaattcac gatatttata
     1501 ttttgaattt ctatgaattt gcccccagta actatgttcc gttataagag cagtaatcat
     1561 cattctgtca gttactttca catcttctgg caaatagtga ggacttgcaa agctatctaa
     1621 actttcatga accattgcat gccaatcagt tgctttcccc cttcgcaatt gcaattctag
     1681 tcgccaccaa gtatcgattt ctttcggaac aactttccgt tttctttctt gttccaattt
     1741 tttgttatac atccgaatct gtcgttcact agcacgactt ccccaatatg ctgtttccag
     1801 tttcccagac cgaccgtaaa ttggtttgaa agaaacagga tcaaccaccc gatattgggt
     1861 aatgaaatca tctggcacgt caataatgtc acaagcaata tctgcacgtg agaaatgtgg
     1921 ttctaaaaat aaatcatgga taaagttctt catcgagccc gccagaaatt ggctaatttt
     1981 attggggtta aagtcaatcc gcccttttcc ctcttgaaac ttaagaatct caatataagc
     2041 tatattttcc tcatgttggt ctagtaattg ccagccatta tttccaaccg cttttaaata
     2101 tccgttacct tcgtttagac gcataagctg ttcaaaatct aaaatcaaca catcatgggg
     2161 agtgtgataa taaatatttt cccttaatgt accaacaatg gtaattcggt caattgacca
     2221 ttgcattttt aagggggtgc acatagtttg tgcatttaac cgcctgttag atatgcggtt
     2281 aagtttttcc cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa
     2341 gaaaaaagag atttttggaa agaatttcca gagttcacgt aactctaaaa atccatatcc
     2401 aaaaatctct agcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac
     2461 taggtgagta gtttccgtaa ttagccgaag ggtgcttcca acaccgttcg gcttttattt
     2521 tgctcttttt tctcgttttc tttgattata gcactcctct taaaaaaata aaaagacctc
     2581 agggaatttc attccccgaa ccccttgagc ctcctcctgc cgaaattatt tcacgcttcc
     2641 gctcttcatt tcattcagtt ctcggcttgc acaatctaac acgccgtttc gctaccagtc
     2701 gccttacagg ctccattctc gcttctctta cgtgttttaa cgattgtgcc gataactaaa
     2761 cgaaatgaac gctaaaacgt cttagaacgc aaaatacgcg cttataaaat catttcatct
     2821 tgattacatg cccgtcataa tcgttcagtt tagtttcatt ttcgtaaact ttttttattt
     2881 ttacgaacag aaaaaagtcg cacatcacca ttttataaag agcataagga aacgtgtacc
     2941 cactttct
//
LOCUS       DAYYNG010000230         2926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000230 DAYYNG010000000
VERSION     DAYYNG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.236"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1625
                     /locus_tag="ACHBFA_002838"
     CDS             234..1625
                     /locus_tag="ACHBFA_002838"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9396244.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1663..2493
                     /locus_tag="ACHBFA_002839"
     CDS             1663..2493
                     /locus_tag="ACHBFA_002839"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9396245.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2465..>2926
                     /locus_tag="ACHBFA_002840"
     CDS             2465..>2926
                     /locus_tag="ACHBFA_002840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9396246.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQM"
BASE COUNT         1018 a          422 c          575 g          911 t
ORIGIN      
        1 tgaatattgt aacttgtgaa taaggaatta gcaggagaat actagttcag cataaaggaa
       61 gtgatgggac ttctgagaca actttctgtt atgcagaaat acacatataa acagacaata
      121 aacgcttttc cccgtttctt ttaatttcct ttacaatttg gttattcact aggtttttaa
      181 actttgctat ggtaagatga tagaggcatt attttatggt taggagtgta tatatgaata
      241 aaaatgggga gtggaatgcg gcacgcagaa tattcattat tcttatggat gtacttgtat
      301 taaatgtatc gatctatctt agttttttgc tgaaattcca gggagaagta ccagtgagaa
      361 acttagaaac ctttcagcat tcagcaattt ttatttccat catctttatt gcgttgaata
      421 ttttgttagg agcttatgta ttctataacc gaatgatcag tgatattgtc ttcattactg
      481 ttattggaca agtactaata gcattgggga tcatggtagt tacttttgct ggccgatggt
      541 ttgcatttcc gcgtggcgtc attttgatct cgtttatcct aggtacgata ttacttagtg
      601 tttaccggat agtcgtttac aaactgtact taagagtcag tcgtgataag aaagtcctta
      661 tcgtgggttt ggagaaagat gtcgcaccag ctatccaaaa ttttcaatct aagaaaaaca
      721 ataaacataa ggttgaagca gtggcaatcg ataattatta cgaaaataca aaaaaattga
      781 tcgatgatat ggatatcgtc tatttggctt ctaaaatccc agaagatgaa aaaatcaaca
      841 tttatcaatt agtgactaaa aaagagaaga aattattttt aaatacgact tttgagaatc
      901 tgacaatgat aaatccgaat attatgaatt ttgaagatga aagcatcatt gaagcttctg
      961 ggttcagaat ccaaccagaa tacgaactat tcaagcgtct ctttgatatc atcgtttcgc
     1021 ttattttact gatcctcgcc tcacctttta tgctaatcac agcgattttg gtaaaaacaa
     1081 cttcacctgg accaatcatc tataaacaag tacgtattac gaaaaatcaa aaggaatttt
     1141 caatctataa attccgttcc atgagtgcga cagctgaagc aaaatcagga ccagtcttgg
     1201 ctaaaagtaa cgatgcacgt gtgacaccag taggtaaatt tatccgtgct gtacgttttg
     1261 acgaattacc tcaaatcttt aatgtattga aaggcgatat gtcgattgtc ggtccaaggc
     1321 cagaaagacc atttttcgtt gatcagttca atgaagaaaa tccttattat tacttaagac
     1381 acaatgtacg tgcgggaatt accggatatg cacaagtata cggaaaatat gtatcagatt
     1441 ataacagtaa actacgtttt gatttgttgt atatcaaaaa gtattcctta ctattagatt
     1501 tgaagatttt acttcaaaca atcaaaatct tatttgataa gatgtcctca caaggactag
     1561 atgaagatga aaatatcagt tcattagcag aaagtgcatt tccaaaagaa aaaacgtatc
     1621 gttagattgg aaaaattttg agcaaagaag taggagagaa cattggaaaa taaaaatata
     1681 agtatactgc tttctttgta tgataaagaa aagccggaat atttaaagga agcattagct
     1741 agtatctttg agcaaacggt gatgccggca gaaatcgtat tggtttatga tggccctata
     1801 aatgagaagc ttcaggcagt tgtcacttat ttccagcaaa aagttcctca cttgtttaca
     1861 gttgtaaaat tagaaaaaaa tcaaggattg gggattgctt tagcaactgg attagaacat
     1921 actaaaaatc gcttagtggc cagaatggat acagatgaca tcatggaacc acacagattg
     1981 gaaaaacaat tagagatctt ccaagaatat ccagaaatag cgattgttgg gagtaatatt
     2041 gaagaattta ctggcacact tagcaatgtt atcggaagaa ggattgtacc tgagtataat
     2101 gatgaaatcc gtgtgttttc gaaaagaaga aaccctttta atcatatgac agtcatgttt
     2161 gataaaaatg ctattttaga agtgggaaac tatcgcccat taattggatt tgaagattat
     2221 tatttgtggg taagactatt gaaggcggga tataaaggat ataatatcca agagattcta
     2281 gtacatgcta gagcaggtgc tgacatgtat gctagacgtg gaggaagtaa atatctttta
     2341 ccaggaatta aaggccgata tcgcgtatgg aaagatggtc taggatcact aaaagatttt
     2401 ctgatagtta tttgcggaca tcttgtaatt agtttattac cgaataaaat taggggaaaa
     2461 gtatatgaat caaaacttag aaaaacaaac taaactttct ataatcatac ctttttacaa
     2521 tgcaaagaat actttaagaa gagctgtaga cagcgtctta acgcaaggtt atcccaatat
     2581 tgaaatcttg ttaattgatg atggatctac agatggtagt agtgacattg gtgacgaaat
     2641 tgctaatcta gagagtaaag tagcatgtta tcacttggaa aacgctggtc catcagttgc
     2701 taggaactat gggataaaag aagcaactgg aaatttggta ggatttttag atagtgacga
     2761 ttatttttta cctgattgtt ttaataatgt tttgaaagac taccatagga atcaccccga
     2821 tatcattgct tttggattaa gtaaaggaaa aaatatagaa aatgcaaatt tatttgttcc
     2881 cgaaaagaag attggttgtt caagtgaaga agcaattgag caaatg
//
LOCUS       DAYYNG010000231         2919 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000231 DAYYNG010000000
VERSION     DAYYNG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.237"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..611)
                     /locus_tag="ACHBFA_002841"
     CDS             complement(69..611)
                     /locus_tag="ACHBFA_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM9396247.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGNIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            746..1135
                     /locus_tag="ACHBFA_002842"
     CDS             746..1135
                     /locus_tag="ACHBFA_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396248.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1139..1903
                     /locus_tag="ACHBFA_002843"
     CDS             1139..1903
                     /locus_tag="ACHBFA_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9396249.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2032..>2919
                     /locus_tag="ACHBFA_002844"
     CDS             2032..>2919
                     /locus_tag="ACHBFA_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9396250.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKG"
BASE COUNT          938 a          490 c          600 g          891 t
ORIGIN      
        1 ataataaata aaaactgtct aacatttaaa atttaattta gatattagac agtttttaag
       61 tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata
      121 tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac
      181 agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc
      241 cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt
      301 ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtttcc taatgccaat
      361 ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat
      421 aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg
      481 atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa
      541 cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt
      601 ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta
      661 agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat
      721 tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca
      781 cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga
      841 ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt
      901 ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc
      961 tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt
     1021 tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac
     1081 tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt
     1141 gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa
     1201 agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa
     1261 ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc
     1321 agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt
     1381 ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa
     1441 aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac
     1501 atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc
     1561 tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt
     1621 accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc
     1681 ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc
     1741 ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat
     1801 tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc
     1861 ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac
     1921 tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa
     1981 atttggtata attgatagta agaatgacaa catgttagaa aggaagagag catgcccact
     2041 ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat
     2101 catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg
     2161 gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa
     2221 ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga
     2281 atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc
     2341 gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg
     2401 atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat
     2461 gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat
     2521 tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa
     2581 actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca
     2641 aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa
     2701 acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat
     2761 gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac
     2821 caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta
     2881 tgtggagaaa tgttgttaaa aaaaatagaa gaaaaaggg
//
LOCUS       DAYYNG010000232         2868 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000232 DAYYNG010000000
VERSION     DAYYNG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.238"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..1054
                     /locus_tag="ACHBFA_002845"
     CDS             95..1054
                     /locus_tag="ACHBFA_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HFM9396251.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="ACHBFA_002846"
     CDS             complement(1487..2059)
                     /locus_tag="ACHBFA_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9396252.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACHBFA_002847"
     CDS             complement(2075..2680)
                     /gene="fic"
                     /locus_tag="ACHBFA_002847"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM9396253.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          921 a          581 c          471 g          895 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc ctctacaaaa ttcacaatag atataatcta ttgaaaattt
     2821 aaaagaagac aaaatagagt acagaaaatg ttttctgtac tctataaa
//
LOCUS       DAYYNG010000233         2866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000233 DAYYNG010000000
VERSION     DAYYNG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.239"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1533
                     /locus_tag="ACHBFA_002848"
     CDS             115..1533
                     /locus_tag="ACHBFA_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9396254.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            1545..2855
                     /locus_tag="ACHBFA_002849"
     CDS             1545..2855
                     /locus_tag="ACHBFA_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM9396255.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          929 a          448 c          600 g          889 t
ORIGIN      
        1 tgttttttat ctgtcaagat aatgcaataa aaagccaaca ttgtgaatat aaaaacaaga
       61 aaaaaagagt tataattttg aaaacgttta ctagaagagg taggaggaat tcaaatgctt
      121 tcacaaaatg ctgttacagc aaatacaata aaaaaagaaa aaaaggaatt tgggatttcc
      181 gataagatag gctacatgtt cggtgatcta ggtaactgtt ttattttagg tttagtcaat
      241 agtttcttga tgatttatta tacgaatgtt cttggcgttt caggggcaat cgtaggtacg
      301 ttattcttga tttcgagaat tttggatgcg tttgctgatg tgatggttgg aagattatgt
      361 gatgtctcag agttaacaga aactggccga tttattcctt ggatcagaaa aatgaagtat
      421 ccattttgct tagtgactgt cattttgttt ttgccattcg tccaacatct tccaatgacg
      481 gtgaagattg cttatatttt tgtttcatac attgtttatg ggatttttct ctcaacaatc
      541 aatatcccgt atggagcaat ggcagcagca attagctcaa atcctaatga ccgcacctcg
      601 ttgtccacgt tccgaagcat tggttctgcg attggcggat caactacagg attcttcatt
      661 ccgattttaa tgtataccca taacaagagt ggacaacaag tcgtttcagg gaatcatttc
      721 ttttggattt caatcggatg tgctgcaata gcatttgtcg catatatgtt aacttgtaaa
      781 ttgactactg aaagagtaag agttgtcaaa aacgaaaaag tatctgcttc tcagttggta
      841 aaaggtttgt tagctaatcg agcactgatt attttagtca ttgttgacat tttcattgtg
      901 atcaatcaga ttttagctgg gacgaatatg acttatttat tcaatgatta cttccataat
      961 aaacaagcaa tgtctgttgc tcttttattt acttacggaa cagtagtagt tttagcacca
     1021 tttgctacaa agctgacaaa attatttggt aaaaaggaag caagcgtatt tgcgttactt
     1081 gcttcttcat gtatgtactt gctgatgtat ttccttcata taacaaatgc atgggtatat
     1141 ctgattcttt tatttatcgc tactttagga tctggtttat tcaatttgat ggtctgggca
     1201 ttcattactg acgtgattga ttatcatcaa tataagacag gcttaagaga agatggaaca
     1261 gtatatggag taaattcttt tgcgagaaaa gtaggacaag catgtgcagg agcggtaggc
     1321 ggatttatgc tggcactcat tggttaccaa tcgtctacaa caggtggagc catccaatca
     1381 gttttagtac aagaaaggat ttatacgatg gcaaacttgt taccagcatt ctgcttattt
     1441 gtatccgcaa tcattttgct agtaggttac ccactgaatc gttcaaaaac tgtatttatt
     1501 ggtgaagaat tgaaaaaaat caatgaaaga tagggagaaa cgtattgcgg acaaaaaaag
     1561 ggattatcaa tgctttgaac tttaaaacaa acgacactca acaagatgat acaataatac
     1621 tgcaaaaagc aatcgatcaa ggtgcgaaag aacacttacc tgtatttatt ccaaaaggca
     1681 tttatttagt cggagccctt tttttaaaag ataagagtca tcttatcttt gaagaaggtg
     1741 cagtcctcaa ggggagaaca gatatcgaag catttccaga aatcgataca cgagtagccg
     1801 gcgtagaaat gaaatggcca gcagcgattt tgaatgttct ctcggcgaaa gatattctta
     1861 tagaaggaaa aggaataatc gacggtcaag gagaccattg gtgggagctg tattggggaa
     1921 aagatcaaaa aagcgggaca agagctgaat acgatcaaaa gggacttcgt tggattgcgg
     1981 attatgctat taaacgtcca cgcgcttgtt tactgtatca tgcagaacat gttgttgttc
     2041 gtgatttgat ttttcagaaa tcaggttttt ggaacttgca aatcacttat tctaacgatg
     2101 tattagtaga aaaagtaatc atccgcaaca atgatggacc aagtacagat gggattgata
     2161 ttgattcttc aacaaatgtg cgtgtctatg aatgtgattt ggcctgtggt gatgattgta
     2221 ttgctattaa gtctggcaga gatggcaatg gtgcaagagt caatcgaaaa tcatcaagaa
     2281 tcgaggttgc tagatgtaaa atccgttcag gatacggtgt aactattggt agcgaagtta
     2341 gtgctggagt tagtgacgta tatattcacg atatcgattt ttttcagtca gactgtggta
     2401 ttcgaatgaa atcttcaaaa gaaagagggg gagtgatcga aaatatcagg gtagaaaatc
     2461 tgaatatgat tgatgtacaa tttccttttt cttggataat ggactggcat aatgaataca
     2521 atagaaaaaa tagcaatgac cttgctcaaa tgccagaaag ttggcaagca gtggctgaag
     2581 aaatacctga atcaaagcaa atgacgaaag tcaaagacat acacatcaaa aacgtacagg
     2641 catgcctttc tccaggttac tcacttcctt cccgagcttt tgatttagta gcatttcctg
     2701 aaaagcctat agaagatgtc tgcttttctc attgccaaat aactgcaaaa gaattcgggc
     2761 gaatcgaagc agtacaaaac ttatgtttca aaagctgtga tttatctata gaaactggca
     2821 acacagctgc aaacaatact tttgacaatc gttgaaatta taataa
//
LOCUS       DAYYNG010000234         2803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000234 DAYYNG010000000
VERSION     DAYYNG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.240"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..497
                     /locus_tag="ACHBFA_002850"
     CDS             72..497
                     /locus_tag="ACHBFA_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396256.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(985..1686)
                     /locus_tag="ACHBFA_002851"
     CDS             complement(985..1686)
                     /locus_tag="ACHBFA_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9396257.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1702..2142)
                     /locus_tag="ACHBFA_002852"
     CDS             complement(1702..2142)
                     /locus_tag="ACHBFA_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9396258.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(2142..2717)
                     /locus_tag="ACHBFA_002853"
     CDS             complement(2142..2717)
                     /locus_tag="ACHBFA_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM9396259.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          866 a          562 c          448 g          927 t
ORIGIN      
        1 ctcttccatc cgtttgaagt tttacattcc aatggtataa tttctttatc ctatcaaatg
       61 ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat
      121 tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt
      181 ctgtcttaaa agcggaaaaa gcagatacca ccaacagtgt atatacgcta aaagtacgta
      241 aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac
      301 gcacgtatca gacacaagga actgattctt ctgaatcatc tgaagaaaaa acagtgctga
      361 cagatatcaa gatcactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa
      421 aagaaacaac agtcatattt acaaaaatcg gtaattaccg tttgaaggat gaggatggca
      481 acatctacag tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg
      541 ctatagtaaa gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat
      601 tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg
      661 agcatatgcg acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat
      721 cctgttcaaa attatgtaaa aagtagaaga agataaacaa atccaagcaa aactcactat
      781 ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc atcagtaaaa
      841 caaacagtgg atcatttcag tttacaaatc aaagagtaaa gatttattta tttcaaatat
      901 ttgaatataa aataaccaaa cgatagaaag gcttattcta catcacgtag taatttttct
      961 ttcccgtcgt ttggtttttt atttttagcc taattcttga ccttttgcat gctggttatc
     1021 cgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt ggatctgacg
     1081 gatcaccttt tttgcatctt ctggattttc tacaaaatcg aatttatccc catccacttg
     1141 tattttagga catacatcaa aatcttcata ccattgatta tagcgggtgt tcaacgtttc
     1201 ataataggta tataacgtct catcgctttc tagctgttca tagtcacgcc cacgtttttt
     1261 gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat caggccgttt
     1321 ttttggagca acatcatcta actcgtttag catcgtatct aataagctat cgtactgctg
     1381 tacttcgata tccgttaccc gacctaaatc agcgtttaga tggaacaaca ggctatcttc
     1441 atagatggat cggtctagca cattgtcatc gttcactaaa gcatttttca ttgctaaaaa
     1501 tcgcttgttc aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat ttgaataaaa
     1561 gagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat caatctcttt
     1621 tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa tcaaaatcac
     1681 tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa caggcaatgt
     1741 ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtatgga gactttgtgt
     1801 aatcatcaag ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt ttttcaacat
     1861 agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct caaagtcaag
     1921 gatagcaaca acaaattctg catttgtcag ttcttccata tctttttcgt atacttcctg
     1981 cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg gagagtagaa
     2041 acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc gatcgatttg
     2101 ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc ctccttcaat
     2161 aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa tggattgttg
     2221 gaaaagatta cttgatccaa ccatttttta tccataaaat cctcttttgc aaaagtttct
     2281 aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat tggcttgtcg
     2341 ggcgtcagac cgattgctat ccaagaaaga agttgtgaga attcctccaa tgtacctaga
     2401 cctccaggga aaacgaggac agcatctccc ttttcaaata ataatacctt tctttcatcg
     2461 atcgtttctg taggaataaa tgtagtaaca tcttttccag gcaattcagt ttcaaaaagc
     2521 ccattcggat agatacctgt gacttttccg ttattttcca gtactgtttt actaatcact
     2581 cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg tgccatgtat
     2641 tttcctaacg tttgcgcaat aaatttgtaa ctttctttat taccaaaacg ggaaccgcaa
     2701 aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt cattctacac
     2761 gtgttttttt aaaataaaag actagatttt gtagtttttt agt
//
LOCUS       DAYYNG010000235         2798 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000235 DAYYNG010000000
VERSION     DAYYNG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.241"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            303..893
                     /locus_tag="ACHBFA_002854"
     CDS             303..893
                     /locus_tag="ACHBFA_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFM9396260.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            920..2230
                     /locus_tag="ACHBFA_002855"
     CDS             920..2230
                     /locus_tag="ACHBFA_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9396261.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2227..>2798
                     /locus_tag="ACHBFA_002856"
     CDS             2227..>2798
                     /locus_tag="ACHBFA_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFM9396262.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGK"
BASE COUNT          958 a          309 c          551 g          980 t
ORIGIN      
        1 ctatagtgaa tttttggaga tttatattcc agttatatat taatgattgt gatacttgta
       61 ctattgtaaa aattcgttag gtttaaataa ctggaaatat cggaaaatgg gcattataca
      121 tcacggacat cccttctttt ggaaaaaatg ttacgatttt tataagtaat attgtatccg
      181 gtggaatcaa cgatgaagat aacatatcca tcgttgtaga ttcagtagca ttgaatgatg
      241 ctcttgttgc aagaaatccg aataaaattg ttaagtataa attgaattaa taggagatat
      301 gaatggaaat tagcagtaaa gaatatttaa aagcggttct tcgtatggaa aagaatctat
      361 atatagaatc taaaggacaa cacacaaaat tactaaaaat aatgaaatca catccggact
      421 acaacgcttg gcggtatact cgtcgaatga gaattacatc ttattattat tcaaagagaa
      481 agaaaaatat catttatgcg attatgtata ttgtgtcttg cagaagaatg aatagtttgg
      541 gaagaaaatg tggtatagag tgtggtgaaa atgtatttga tgaaggtgta agattttacc
      601 atacacaagg aattgttatt aatggtaacg ccataatagg aaaaaattgt tacttatatg
      661 ggaataactg tattggaaat gatggaatcg atcctaagtg tcctgtaatt ggaaataatg
      721 tacgtgtatg cgtgggtgca aagattattg gtaatgtaaa aatagctaac aatgtagttg
      781 ttgcggcagg agctattgtt atcaaagatt gtctagagaa tggtgcaatt cttgcaggtg
      841 taccggcaaa aataataggg tatgttaatt taacaaaagt atttttagaa taatttttag
      901 cgaaagaagg tgaataacga tgcgtatttc aataaaaaga tggtcaggag cagattggac
      961 attgttcacg ttggcatttt ttgcaatctc catagttctt gtatatactt ttaacgcccc
     1021 aagagcaatt ttatatttgg gagatataat taatatcttt gtgttattaa atgcacttag
     1081 ctttagacgc agaaaaggaa tatatgctag caaaaatgag atgctgatag ttttgttttt
     1141 actagtttct atttgtagct cagttattaa tttagaaaat ataggattga ttatctgggg
     1201 gatacgaaat aacactcgta tgttcatagt atatataaat tgtgcaacat atttaacaat
     1261 taaagattac tataaagtat tcgctataat tgaaaaatta ttctggattt cactgccact
     1321 ttgtttaatt gaaagattca tggtcaccta tccaactgga acaattgtag gagatatgat
     1381 aggtggagtt ttttggaatt attcaggttg taatatgcca ttgaatttaa ttttgtgtgt
     1441 gacagttact gttgttacaa tcggatattt caagggagaa aaaaatttca tccattttat
     1501 tttatgtgtt tgtgcagcac tttttatgtc tgcattagct gaattgaaag tttttattcc
     1561 tgaattattt ataatatttg ttgcaatagt attcacttta aaagttgatt ttaaagtagt
     1621 gatcaagata ttatcatgcg taatacttgt gtcgatagta ttatcctttt ttataacaat
     1681 atttattaca ttgaacgata atggacaaag ctatgctgat aatttcacaa ttcaaggtct
     1741 tttagaatat gcgtcaagag attctggata tgatggtgtt ggtgatttaa atcgaataac
     1801 tggtatctct agagtagtag atgaaatatt taataataat tttagattag tcctaatggg
     1861 agtaggcatt ggcaatgcag agtttaccaa tttttttgtc agtgagtttt ataaatcgtt
     1921 tggtaatctt cattatcagt attttcatca tatttggtta tttatagaag ttggaatatt
     1981 aggagtttcg atattcctat tttttataat aaagacattt ttcaatgcaa aaagatcttt
     2041 tatgaaggat aaagatgcaa gtcttgttaa agttattgca attatgtcgg tattcttttt
     2101 tgtgtataac acaacactta ggggtgaaac aacggggcca ctgatttata tgattttagc
     2161 catgcctgaa gttgtgaaaa ggaaagagca ttattatagt gaaaaaaata caaaaggaga
     2221 gatattatga ttcctaagaa gatacattac tgttggttcg ggggaaatcc acttccgaaa
     2281 aaagatagaa agtgcataga aagctggaaa aagtattgcc ctgattatga gattattgaa
     2341 tggaatgaat caaattatga tttaaataag aaccgttata tggccgatgc atataaagaa
     2401 cgtaaatggg gttttgttcc agattatgca agatttgata ttatttataa tcatggtgga
     2461 ttttatttag atacagatgt tgagctaatt aagtcgttag atatacttcg tgaaaatcat
     2521 gcctatatgg gatttgaagg ggaaatctgg gttaatggcg gtattggttt tggtgcagaa
     2581 gaaggacatt ctgttattaa aggtttaagg gatatttacg attcattaag tttttatcaa
     2641 aatgatggta cattgaactt gaaaccgagt ccgtattata ttacagatta tcttgaatct
     2701 attggattgg ttcgcgataa cagcgaacaa ataataaatg gcctgcatat ctatcctacg
     2761 gattattttg ctgccaagga ttatgatact ggaaaaat
//
LOCUS       DAYYNG010000236         2749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000236 DAYYNG010000000
VERSION     DAYYNG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.242"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..325)
                     /locus_tag="ACHBFA_002857"
     CDS             complement(5..325)
                     /locus_tag="ACHBFA_002857"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9396263.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(433..720)
                     /locus_tag="ACHBFA_002858"
     CDS             complement(433..720)
                     /locus_tag="ACHBFA_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396264.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            847..1491
                     /locus_tag="ACHBFA_002859"
     CDS             847..1491
                     /locus_tag="ACHBFA_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9396265.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(1550..2257)
                     /locus_tag="ACHBFA_002860"
     CDS             complement(1550..2257)
                     /locus_tag="ACHBFA_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9396266.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(2477..2647)
                     /locus_tag="ACHBFA_002861"
     CDS             complement(2477..2647)
                     /locus_tag="ACHBFA_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396267.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT          827 a          580 c          463 g          879 t
ORIGIN      
        1 ctatttattc acctatgagc gtgattgctt gatctaacac tttttcgcca ttcatcatac
       61 cataatcagc catattgatg acatctaaag gaatgccttt tggttccaga cgtttctcaa
      121 aatcagcttt cataaagcga acttgtgggc ctaataacaa cacatccacg tctttatttg
      181 caaggttgtt atctgcgtca gaagcggaca cagcgaaaat atccgagtcg attccttttg
      241 cttcagcagc tttttgcatt tttgttacta gtaaacttgt gctcatacca gccgaacata
      301 caagcatgat tgttttttta gccatacttt tctctccttt ttaaaacaaa ctatgcaaac
      361 gttttcttcg cgtctgttac tctattatat aaaaagaaag aaatttgtca acgttttcta
      421 aacgcggttt atttaatcaa atagccaact agatttcact aatttattga acacgacata
      481 ttctttgtcc aaattgatga caactacttc atagcctgca tttatccagc cataggcacc
      541 tttttcaggg tgtttataat catttgccca ccaatctgta ctttcccttg ctgttttggg
      601 aagtccgtta ctgggaaaca gcagttcgtc aaattgggta aaggtcaacg ttacttgaga
      661 gccgccattg ttcttcaaat aatgcgtaat tgcatcgtac tttttcattc gttcctgcat
      721 ccccagatcc acctgccttt gtctttatta tcgcatacta cagcaattgc gcaaccgtta
      781 cagtgttgaa acaacctaga actttattta tactgaataa agaaaatgaa aaaaagaggt
      841 gacaagatga tcgagcgcta tcgtacaata aaagaaaatg gacaaagtga gattgagatc
      901 aaaaaatcac gctttatctg ttctcttaga cgaatagaat cagaggacca agcaaaaagt
      961 ttcattcaag aaataaaaaa agaacattgg aaagcgaacc ataactgcag cgccttcatc
     1021 attggagaaa aaaatgaaat ccagcgaagc agcgatgatg gagaaccaag tggaacggcg
     1081 ggcgtaccga tgttggaagt cctcaaaaaa aatcaactga tcaatgtagc tgcagtagtc
     1141 actcgatatt ttggaggaac gaaactcggc acaggcggat taatacgagc atacactcat
     1201 gcggtctctc aagcattgtc tgacattgga atcgtagaag gtaccttgca acaggagatc
     1261 tctgtgatga tcacgtatcc gcaactagga aaattacaga attatcttga actccaacat
     1321 ttttttataa aagaaatcca atatactgat caagttagcg tcatctgtgc tgtagatgaa
     1381 aaaagtgctg accactttaa agaatccatc attgagctgc tgaacggaca agtccaattt
     1441 gaagagggtt ctgtttctta tcacgagcaa ctgataactg atcatatata aaaacaggtg
     1501 attctgattc tcttttattt ctaataagga atcagggatc acctgttttt tatttttcca
     1561 aataaaactc aaaacgattt cctacgtatt gcgtacgcac atactcaaac ggcgtaccgt
     1621 cttcaaaata agaaatctgg cgcaaacgaa gaactgcatc tcctcgtttt acctctaaat
     1681 agtcagcaat tttctcagaa gccaacacag cagtaatcgt ttgattcgct ggaccgatct
     1741 ttttcccgcc tttttcttct agcgtgcgat acaacgattc agtaatctct gctttgctgt
     1801 agccatcgat cagcttttgt ggaacactgg caacttcaaa acaaataggg acatcatctg
     1861 cgaaacgaat acgttccatt cgcaagatcg tgtcatcttt actcaagttt agtttttcca
     1921 tttcacttga gcttggcgaa gtcaagaaat aagagattgt ccgactagac ggtactcgat
     1981 tttgtgattc cataatttct gtaaaacttg ttgcaccaga cattttttct tgtacttttt
     2041 ctcgcgcaac atacgtaccc gaaccgatct ttctttctaa gattccttca tctgccaagt
     2101 tttgaattgc ttgtctcaac gtcatacggc tgacattgaa tttcactgct aattctcttt
     2161 ctgaaggaag acggtctccg actttccata taccctgttc gatttcattt tttaattggt
     2221 catgaatctg tatatataca ggtaattgat tcgacataac gtacacccgt cctttcgttt
     2281 cttctattat aactggtata tacctgattc acaactgaaa aagataaagg tttctatcca
     2341 tcaacagagg atttagacac acatcgttct ccctactcct attgtcagaa acaatttttt
     2401 agatatgttc aacctagaaa ataacaaaag accctaaaat cgatgaaaat gccgacttat
     2461 ggatcttttg ttattattag cggtttttag ccatccattt ttttgtatga ttcgttgggc
     2521 gttttgattt tacctggacc gcttttccgc cttttttcgg aatattcact tcaatattgt
     2581 atttttctga tttgaccaaa tcatataact tttcagctgc ttcgtctact ttttctttcc
     2641 atttcatgtg gatccctcca attcttgtag ttaacttaac tctatcttaa ttcagcagaa
     2701 tcttcaagga acaaggtagc aaagcaaaaa aaagagctct cgaactaac
//
LOCUS       DAYYNG010000237         2732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000237 DAYYNG010000000
VERSION     DAYYNG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.243"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..515
                     /locus_tag="ACHBFA_002862"
     CDS             171..515
                     /locus_tag="ACHBFA_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9396268.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACHBFA_002863"
                     /pseudo
     CDS             516..935
                     /locus_tag="ACHBFA_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ACHBFA_002864"
     CDS             complement(1027..1377)
                     /locus_tag="ACHBFA_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396269.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ACHBFA_002865"
     CDS             complement(1343..2674)
                     /locus_tag="ACHBFA_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM9396270.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1013 a          493 c          527 g          699 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca ac
//
LOCUS       DAYYNG010000238         2692 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000238 DAYYNG010000000
VERSION     DAYYNG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.244"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..915
                     /locus_tag="ACHBFA_002866"
     CDS             61..915
                     /locus_tag="ACHBFA_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9396271.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1012..>2692
                     /locus_tag="ACHBFA_002867"
     CDS             1012..>2692
                     /locus_tag="ACHBFA_002867"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9396272.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIE"
BASE COUNT          978 a          372 c          497 g          845 t
ORIGIN      
        1 atgtaataat ataaacataa taaaacaaaa attatccaaa tctaacatag gagggtggat
       61 atggaaaatt ctatatattt agtaacagaa cctaacactc agctgaaaga ttgttacttt
      121 aatttttgtg ggtattcaaa aacagaacct agacatagtt ttggaccagc tattcgagaa
      181 caatacttaa tacacatagt gctagaaggt gaaggctatt attcaattaa tgatcaaaaa
      241 tattttctga agaaaggcca aggatttgtt atcccaccag gagtatctac tttttatcaa
      301 gcagatgaaa aaaatccgtg gtcatatgta tggttgggtt taagtggcaa tcttgtagaa
      361 tactatctaa aagaaattgg tgttggaaag aatcatctta cctttaaagt cgaaaattta
      421 aattcattta aaggattaat ttttgaaact ttatcgtatg atcaggacaa tctagtcagt
      481 gaattgtcca gacaaaaaca agtttataaa tttttagaac tcctcttaaa ttcagctagt
      541 tatctgaacg aagggataat tactaaaaag atgaatcctt ttgttcgtca aacaattgaa
      601 ataatacgtg agaatgcgta tcaaaaatta tctgttaaag atattgctgt cagtctatcg
      661 atacacgaaa attatttatc acgattgttt aagaatgata tgggaatatc aataaaagag
      721 tatattagtg aaatcaaaat ttctattgca aacgatctgt tatcatcaac tgaatattca
      781 atccagcaaa tcagtgacaa gataggattc tcaacagtac aatctttttc taaagccttt
      841 aagaaagcaa atggtgtttc accgggagta tatcgaaaac gtagattagg tctaggaatc
      901 attaatggtg aataaactaa acatttgatt ttgataaata ttcgttactt taaacgctta
      961 ctatttatgt gagcgttttt tataatgcaa taaagaaacg gaggagagct tatgtataag
     1021 gataatgaaa tgaatattat ttatcgatca gaaaaccaac aatttcattt gtttaatcag
     1081 gagataagtt acatttttaa gatatcggaa gatggtaaat tattgcattt atattatggt
     1141 gagaacttac cagataatga ctatagtcat ctaattgaga tgcatcatcg gcctatgaca
     1201 acctatagaa atgaaaacga tttgctatat tcgttagagc acttaaaaca agaatttcct
     1261 gaatatggta caacagattt tagacatcct gctatttcat tactacagaa aaatggctct
     1321 cgcttatcag attttgtcta tcaagggtac gaagtggaag aaggaaaacc taaactagaa
     1381 ggtttacctg ctacctatgt tgataataat gatgaaagca aaacattgaa agtttatatt
     1441 ttagattcag ttacaaatgt tcaagtagaa ttattatata caatttatag agattatcct
     1501 gttattactc gtagcgcgcg agttatgaat aaaggaaaag atattcaaat aatcgagaat
     1561 attagcagtt tatcattgga tttacccgat gcaaattatg aatggcaaca attatctggt
     1621 gcttggggta gagaaagaac tattaagtcg cgtactttac aacaaggaat tcaatcaatc
     1681 gaatctactc gaggtatttc aagccatcag cataacccat ttgttacttt aaaaagaaaa
     1741 aatactgatg aaaatcaagg agaagctatt ggtgcagcac tagtttactc aggaaacttc
     1801 ttaattcaag cagaagtaga tacatgggat gttactagac tacaagttgg catcaatcca
     1861 tttggattta attggaaact caatccagga gaaatattta cagcaccaga agcgattctg
     1921 gtttattcaa gtaagggttt aaatgccatg agtcaaactt ttcatcaact atttagaaaa
     1981 cgcttagctc gtggtaagtg gcgagaaaaa gaccgtcctg tcttaattaa taattgggaa
     2041 gcaacatatt ttgattttga cgaagaaaag ttggtaaata ttgctaaaaa agcatttgat
     2101 gttggtattg aattatttgt attagatgat ggctggtttg gagaaagaga aaacgattgt
     2161 gcaggattag gagattggca tgtaaatcct aaaagactac ctagaggaat cggcagcctt
     2221 tcagaaaaaa ttcgtagttt tggtatgaaa tttggtttat ggtttgaacc tgaaatggtg
     2281 aataaggata gtgagttata tcgcacacat ccgaattgga ttatttctac acctaatcga
     2341 aaacctaatc atggtaggaa ccaatatgta ttaaattttg ggttagatga ggttgttgaa
     2401 aatattttcg atcaaatgtg taaaatcatt gatgaatcaa acttagatta cattaagtgg
     2461 gatatgaatc gtccattgac tgatgtattt gacagtcact tgtcagcaga tcaacaagga
     2521 gaagtattcc acagatatgt actaggcgta tatcgcttat atgaaaaact tataacaaaa
     2581 tatcctaaca tactatttga gtcttgttca tcaggtggtg ggcgctttga tcctggaatg
     2641 cttcactatg cacctcaagc ttgggctagt gatgattcag atgcaattga aa
//
LOCUS       DAYYNG010000239         2652 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000239 DAYYNG010000000
VERSION     DAYYNG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.245"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACHBFA_002868"
     CDS             137..748
                     /locus_tag="ACHBFA_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9396273.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACHBFA_002869"
     CDS             821..1750
                     /locus_tag="ACHBFA_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFM9396274.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACHBFA_002870"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACHBFA_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
BASE COUNT          930 a          369 c          493 g          860 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aa
//
LOCUS       DAYYNG010000240         2579 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000240 DAYYNG010000000
VERSION     DAYYNG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.246"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1614)
                     /locus_tag="ACHBFA_002871"
     CDS             complement(100..1614)
                     /locus_tag="ACHBFA_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM9396275.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            1752..2558
                     /locus_tag="ACHBFA_002872"
     CDS             1752..2558
                     /locus_tag="ACHBFA_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396276.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT          697 a          555 c          423 g          904 t
ORIGIN      
        1 ctttttcaca acctcttcac taaacagtgt tcaatcagct gaccctcggt attagctcaa
       61 aacgctgatt tcacaacctc tttttattta cgttctattt cattcatttt ccgaagcaaa
      121 tcgagctgct tgtatgcccg cttgtcgccc aaaaataatg atctccgcaa ctgaatttcc
      181 accgatccgg ttgtttccat gtaagccacc tgtgacttca ccagcagcat ataaccccct
      241 aattggctgg ttttctttat ctaatacttc agtgttcgta ttgattttca caccgcccat
      301 cgtgtaatgg attcctggtg cgattttgat cgcataataa ttcggagacg ataagtcatg
      361 ttccatggct gtggcacggt tgaattgagc atcttttttc tccgcaacat ccgtattcca
      421 tccatcaact gtttttgcta gtgtttcttt tggtaaatcg attttttctg ctaattcttc
      481 cattgtcttt ccagttgtga catatccttt ttggtcataa aattcaatag ctgtggcacg
      541 atcacgcacg ccttggtcaa aaatcaaata agctgatttt tcaggcaatg ctgtaattgc
      601 tgcagatact ttatcccttg tatccatttc attgacaaat cttttcccct cttgatcgac
      661 caagattgct ccttctccgc gaacaacctc accaatcaag accccctcat cctgctgcac
      721 agttggatga atttgtattt tatccatatc aacggtttgt ccacctaatt tttcaatcat
      781 ggtgatacca tctccagttg atccttcttg atttgtcgta atataatcag ctaaatcggg
      841 acgatatttt tcaataaatt ctttgctggc accaaaaccg cctgtggtaa ctacgattgc
      901 ttttccggtg acctcttttg gttcttctcc ttgaacatgt acagtcactt cattgaccgt
      961 ttccccattt ttcttaatgt ctgtcacatc tgcgttgaca aaaataggga tttcccgctc
     1021 atggacgtta ttcaataacc cctccactaa gtatccacca atagccgagc catcagacgg
     1081 acgatgtgtc ctcttttcac tcatccctcc agtgatcgtt aagttgtcca gtttgatacc
     1141 gttttgatct agccaatcga ccgcatcagc agagtgatcg acaaagtatc gaagcatttc
     1201 tttatcattc gttcctccac caccttttag cgtttcttca tagaaggcat cattcgagtc
     1261 tttgattcct tcttcttttt gaaattttgt ttctgaagca ttcataccgc ttgatgattt
     1321 agatgtgttt cctccggcaa caggcatctt ttccaaaatt actggattca acccagcatc
     1381 ttttgcttcg atagctgctg ccatccctgc accaccagct ccaataatca cgacatcata
     1441 agattctttc atttcttttg gatctgtata tacttgttca gaagcgcctg atgatacttc
     1501 ggcagattcc ttcgtactag aacttgtctg cgctgtattt ttctgatctg cgccgcatgc
     1561 gctcatagca agaagtgcaa cggctgttaa taaaccagca actaattttt tcatttgtat
     1621 cccccttttc tccttatact atattaagtg atttttttca caatatcaac tctgtttttc
     1681 tttaagtcat atagctttgg taaacacacg cttcttagta taattaagga tatacatgaa
     1741 ggagttattt tatgaatcat ttgaaaacct tttttacgaa cagtacaaaa actgcaggta
     1801 gaatcacaaa cggaatctat attttattac gtatcatcgt cgttattatg ctagtggatc
     1861 aggtctggaa ccaacactgg ggaaatacaa tgcttttatt actgacatta attttatttg
     1921 aaatccctta ttttctggaa aagatattaa agatacagat tccaaatatg ctagaaatca
     1981 ttttgttatc ttttattttt agttcaacag ttttaggaga gttaagtgat ttttatggct
     2041 atttttcaat gtgggatact tttctacatg cattcagcgg atttttagct gggggtgtcg
     2101 gcttttccct tgtttatttg ctcaataaaa atacaaaaac catgaacttg actcctttat
     2161 tactagcagt tgtttccttc tgtttttcga tgacagttgg tgttatgtgg gagtttgtgg
     2221 aattttcagc agataaatta ctgcaattcg atatgcaaaa agatacgatc atccagcaaa
     2281 tcaatagtgt ggagttaagt aaagacggaa acactgtcta tcatgttcca gatatcactc
     2341 ggacaatcat tgaaagtcaa aatgcagatg gtaaaacaga taaaacaatc atccaaaatg
     2401 gttacttaga cgtgggaatc atggatacta tgaaggattt atttgttaat tttattggag
     2461 cagttgtttt cagtgtattt ggttacctct atagtcgaaa cgattaccaa aaatttacgt
     2521 tcatcaagaa ttttattcct aaaaaaatca aacgataaaa catgctaaaa aacaagaga
//
LOCUS       DAYYNG010000241         2413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000241 DAYYNG010000000
VERSION     DAYYNG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.247"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..1904
                     /locus_tag="ACHBFA_002873"
     CDS             324..1904
                     /locus_tag="ACHBFA_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9396277.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2102..2326
                     /locus_tag="ACHBFA_002874"
     CDS             2102..2326
                     /locus_tag="ACHBFA_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM9396278.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          870 a          385 c          508 g          650 t
ORIGIN      
        1 aatatttgac tcttatggtt tggtggcttt ttcattcgat gaatccatgc tatactgatt
       61 cagttgtagt agttgacaaa ggttgtgaaa aagctatcct gcattaagta ataagagcag
      121 ccaagcaaaa acagcttttc gtatttttgg cttgggtggg cttattgctg ataggctgtc
      181 tctagaacgc cgtttactcg gaaaaagaga ctgctacacg gcttttgtca cagccctata
      241 tgaaattgtc tttctttaag agatcggaca atctgtgcct agagaaaata taaataattg
      301 aatggaagaa atggagttag gttatgaata atccagaatt aaagcaacaa gtggacaatc
      361 gcagaacttt tgcgatcatt tcccatccgg atgctgggaa aacaacgatt acagaacaat
      421 tactattgtt tggtggggct atccgtcaag ccgggaccgt aaaaggcaaa aaaacaggta
      481 attttgccaa atcagactgg atggaaatcg agaaacaacg tgggatttct gttactagtt
      541 ctgttatgca gtttgattat caagggaaac gagtaaatat cttggataca ccaggacatg
      601 aagatttttc tgaagatact tatcgtactt tgatggccgt ggatagtgcg gtcatggtaa
      661 tcgatagtgc aaaagggatc gaagcacaga caaagaaatt attccaagtt gtaaaaaaac
      721 gtggcatccc gatatttact tttatcaaca aactagatcg tgatggtcgt gaaccattgg
      781 aattgttaga agaattagaa gagctattag atatcgaatc ttatccgatg aattggccaa
      841 tcggtatggg taaagggcta gaaggtcttt atgatatcta ccataatcgt gtagagtttt
      901 atcgtccgga aaattatcag gatgaacgat tagtagaatt ggatgaagat ggactgcttc
      961 ccgaaaatca tcctctaact gaaaattcct tatatgaaca agtcttagaa gaagtagaac
     1021 taatcaaaga ggctggagat gcatttaatc aagaaaaaat tgctcgtggc gatcagacac
     1081 cagtattttt tggttctgca ttgactaact tcggtgtaca gactttttta gagactttcg
     1141 ttgaattggc accagctcct tatggacaca aaacacaaga tgatcagatg gtaagtcctt
     1201 atgaggaaga attttcaggt ttcgtattca agatccaagc aaatatgaat ccagcccatc
     1261 gtgaccgaat cgcttttgtc cgaatctgtt ctggaacatt cgaacgagga atggatgtct
     1321 ggttggaacg tacaaataaa aaactgaaat taagcaatgt cacacaattt atggcagatt
     1381 cgagagaaaa cgtcgaaaaa gcagtagcag gcgatatcat cggtgtatat gatacaggca
     1441 attatcagat tggggataca ttgttcgaag gaaaactaaa agttgcttat gaagaactcc
     1501 cttcatttac tccagagctg tttatgaaag tgactgcaaa aaatgtcatg aaacaaaaat
     1561 ccttccacaa aggaatctat caattagttc aagaaggagc gattcagctt tataaaacat
     1621 atctaacgga agaatatatc attggagctg ttggtcagct acaattcgaa gtattccaat
     1681 atcgtatgtt aaatgagtat aacgccgaag taatcatgtc tccaatggga cacaaaatcg
     1741 ctcgctggat cgatccagaa gatttggatg agaagatgag ttcaagtcgc aatatcttgg
     1801 ctcgtgaccg tttcgaccaa ccattgttct tattcgagaa ccaatttgca gaacgctggt
     1861 ttgcggataa atatcctaat gtaaaattaa aaagtttgat gtaaagaagt cttgacacaa
     1921 gctgccctgc atcaagtaat aagaatagcc aaacaaaaac agcctctcat attttgttta
     1981 gagccgtgac ggattttccg tgacggctct ttttactata ttaaaatcat agaaatgtct
     2041 aatcaaaaaa atataaaaat tattttgcgt acatatgatt aaagaaagga gagtgtgaca
     2101 catggaaaca gaatggcaaa tacgtgaaag aaaaaaacag aaaaaagaag agatgttacg
     2161 attatgtcga ttattagatg taaaagactg ggacaaaaac gagcaaattg cacaaaaagt
     2221 acgatcaatc atccataaag aggaaaaagt taaaaataaa gacacgcgta gaactgaaaa
     2281 tgaaaagaga aaagtacaaa agaaaagaga aaaatcaaaa agctagaaag aaaaagaggc
     2341 tgagacagaa gcgtttaact ccaagaaata agctggaatt cacaaaaatt tgaaaagcaa
     2401 ttttcgtgaa ttt
//
LOCUS       DAYYNG010000242         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000242 DAYYNG010000000
VERSION     DAYYNG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.248"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHBFA_002875"
     CDS             316..570
                     /locus_tag="ACHBFA_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9396279.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHBFA_002876"
     CDS             571..888
                     /locus_tag="ACHBFA_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9396280.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHBFA_002877"
     CDS             1447..2382
                     /locus_tag="ACHBFA_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396281.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYNG010000243         2377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000243 DAYYNG010000000
VERSION     DAYYNG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.249"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACHBFA_002878"
     CDS             complement(4..249)
                     /locus_tag="ACHBFA_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396282.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACHBFA_002879"
     CDS             complement(266..1117)
                     /locus_tag="ACHBFA_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9396283.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACHBFA_002880"
     CDS             1283..2287
                     /locus_tag="ACHBFA_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9396284.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          758 a          402 c          478 g          739 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaa
//
LOCUS       DAYYNG010000244         2304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000244 DAYYNG010000000
VERSION     DAYYNG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.250"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..2259
                     /locus_tag="ACHBFA_002881"
     CDS             115..2259
                     /locus_tag="ACHBFA_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9396285.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT          804 a          487 c          441 g          572 t
ORIGIN      
        1 acagacagat ttatgaaaag aaaaagcttt tcgacacgat tatctgttcc aaaaaatgtc
       61 taatgaatgc tgaataagac agaaaatcaa tgaaaggaaa aggactagtt tattatgaaa
      121 aataaaaaaa cgatacgttt gctagctgct acttggctga ttgctccgtc tgttttgtcg
      181 accaataccg tatttgccgt agataatcaa acagctgatc aaacagtcac aacagatacg
      241 actacatcca gctcagctac tagtgaaaca acggattctt ttacagaaga cccaactgga
      301 acaactaact caacaacagg atcgaccgag ccaactgaaa ttgatccggc tgatccatca
      361 gaaaatgaaa agccagccga gacatattcc cttacaattg ctccttcttt gattggtaag
      421 atccaactga ctgcttcgac agcggattcc cctgaacagc aacctttgga tattacggaa
      481 agtggaacgg tagaaggttt attagcggaa acgaaggtaa cttacactat tacgccagct
      541 gaaggtgaac aaattgcttc agtcgctgtt aatggtgcca aagaagtaaa ttatgatgca
      601 aagaccttta cagtaggtac gaacaattta tctattactg cagaatttga acctgtaccg
      661 gatacttcaa cagagaaacc aactgatcct agtgaagaag tccctagtac accagatccg
      721 gaaacgccaa acaaaccaga tgtgggtaat aacaacaata ataatggttc aaataattca
      781 aacaataata acaataattc gaacagcggg aattcttcga atcaaacaaa cccatctgga
      841 tcgaataaca atggaaatac tcagagagaa ccaaaccatc caaacaatgg ttcatcaaat
      901 ggcggaacga acaatgcttc atctcgtcca aatacagcaa atcaaagcgg tatagagaat
      961 ccatcttctg cttcttctga ttttgttgta aaatcaccaa ttgatgctgt attgcctaca
     1021 aatacaacga atgtccaaca agcaatcgtt cgagaagcat tcaagcatct tggaaaacca
     1081 tatgtatggg gagcaaaagg tccaaatact tttgactgtt ctggtttgac ttattacgta
     1141 tatatgaaag caaccggtca ttatatcggt ggctggacag gcgaacagca atatgcaggt
     1201 acacagattc cagtaagtca ggcccaacct ggtgatctcg tcttctgggg accttcttct
     1261 ggtgttaccc accacgtagg tatctatatt gggaacggac aatttatcca tgcgcctcaa
     1321 ccaggagata aagttcgagt aacttctatt tcagatttca cgcctgattt tgctgtccga
     1381 gtaaacttag caggtttacc tgctgcttca ggttcgctga caggcggctc atctatttta
     1441 gatggattag atggctcgtt ccatttcagt caaaaccaaa caactgatca gttcttgaaa
     1501 aagattgcag atgatgcaca ggaaatcggg caaaaagaag gaatctacgc atcagtcatg
     1561 atggctcaag ctatccttga aagtggatct ggaaacagct tattatcaag cgaaccaaat
     1621 cataaccttt ttggtatcaa aggatcatat aaaggaagca gcgtgacctt caatacactt
     1681 gaacaagata gttccggaca aagctaccaa atccgcgcac aattccgtaa atatccttct
     1741 tacaaggaaa gtttggaaga ctatgccgac ttgatcaaaa atggactgac tggcaatcct
     1801 gatttctaca aaccaacttg gaagtcggaa acaaaagact acaaagaagc aaccaaatat
     1861 ttggaaggac gttatgctac agatcgccaa tattcacaaa aattgaacgc gatcatcgaa
     1921 gcgtacgacc tgacaaaata tgatgaaccg aaaaaagaag cagcaacaac tgaagaagca
     1981 aacgctgaaa tcctcaatat gtctaagcga ttcgatgtgc caatcaaatg gacaacagat
     2041 ggcgtgccaa tcacaggatt agaattttca acgataaaac gcgtcaacaa cgcttcttcc
     2101 atcttccgtg tcgcttcaat ctgggatttg tggaacaatt ttacggcaag acagatacca
     2161 gaaacaacta ctcgtacagt taaagctgca actgttcctg tactaacctt gttaagttta
     2221 gacaaatcat tctggcttga tctgccttta aagaaataga ataaattaaa aaggggatgt
     2281 gacactgtca cactcccttt ttaa
//
LOCUS       DAYYNG010000245         2298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000245 DAYYNG010000000
VERSION     DAYYNG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.251"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..1745
                     /locus_tag="ACHBFA_002882"
     CDS             132..1745
                     /locus_tag="ACHBFA_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9396286.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          718 a          408 c          447 g          725 t
ORIGIN      
        1 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
       61 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
      121 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      181 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      241 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      301 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      361 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      421 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      481 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      541 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      601 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      661 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      721 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      781 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      841 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      901 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      961 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
     1021 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1081 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1141 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1201 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1261 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1321 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1381 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1441 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1501 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1561 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1621 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1681 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1741 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1801 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
     1861 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
     1921 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
     1981 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
     2041 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
     2101 ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac
     2161 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
     2221 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
     2281 gttcctattt atttaaca
//
LOCUS       DAYYNG010000246         2289 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000246 DAYYNG010000000
VERSION     DAYYNG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.252"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..1958
                     /locus_tag="ACHBFA_002883"
     CDS             96..1958
                     /locus_tag="ACHBFA_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9396287.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGCQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1958..>2289
                     /locus_tag="ACHBFA_002884"
     CDS             1958..>2289
                     /locus_tag="ACHBFA_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9396288.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          823 a          340 c          451 g          675 t
ORIGIN      
        1 taaaaagcta taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga
       61 aaacgcttat attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa
      121 ggcagctttt gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg
      181 aaaaagaaaa tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc
      241 tgaatgagcg gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta
      301 gtagctctga tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat
      361 ttgcagatca aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag
      421 ctattactgg aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg
      481 atttagatgt gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc
      541 gaaagggttt ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc
      601 ttcaaaaaga gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg
      661 gtgagactga atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta
      721 atcaagtaat cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt
      781 acgcggaatt actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag
      841 aggctacgat tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt
      901 cttatcgcgt catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat
      961 atcattatat ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag
     1021 ttgagaatgt gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac
     1081 aattgttgac gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg
     1141 tcaatgaata taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg
     1201 cgatcaagca agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt
     1261 tcgtggcata tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa
     1321 gcgttagagc cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa
     1381 agatcaatga agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc
     1441 aagaaatgat tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca
     1501 tcgatcgtcc tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc
     1561 aacaaataat aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc
     1621 ttagagaaac gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga
     1681 aagcttatac gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat
     1741 atcgagaagc ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac
     1801 aatatatggc agaaggttgt caagtagacc agattatttt agacaaagga acactggtag
     1861 gaaaaatcga agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg
     1921 cattattctc atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt
     1981 aaaaattatc gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga
     2041 agagcgatta gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa
     2101 tcacttgaat gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg
     2161 ggatttgttc aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag
     2221 tatcggtaat ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa
     2281 aaataatta
//
LOCUS       DAYYNG010000247         2230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000247 DAYYNG010000000
VERSION     DAYYNG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.253"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACHBFA_002885"
     CDS             <1..375
                     /locus_tag="ACHBFA_002885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396289.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACHBFA_002886"
     CDS             complement(324..893)
                     /locus_tag="ACHBFA_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9396290.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..>2230)
                     /locus_tag="ACHBFA_002887"
     CDS             complement(967..>2230)
                     /locus_tag="ACHBFA_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396291.1"
                     /translation="NAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDRHLKRLAESD
                     CPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYEQKYDRSTQE
                     RLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQSFSEMFIQLL
                     KQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSCNQITEKRWK
                     RIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVIGIHDRLIEE
                     VRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGYLDVHPPEVK
                     IVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNE
                     DQKTYLYHPSFGIERLLTDINYWLGKNR"
BASE COUNT          622 a          454 c          396 g          758 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt
//
LOCUS       DAYYNG010000248         2218 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000248 DAYYNG010000000
VERSION     DAYYNG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.254"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACHBFA_002888"
     CDS             complement(51..1598)
                     /locus_tag="ACHBFA_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9396292.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHBFA_002889"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACHBFA_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9396293.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          622 a          464 c          397 g          735 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaa
//
LOCUS       DAYYNG010000249         2177 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000249 DAYYNG010000000
VERSION     DAYYNG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.255"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(558..674)
                     /locus_tag="ACHBFA_002890"
     CDS             complement(558..674)
                     /locus_tag="ACHBFA_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9396294.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(1150..2028)
                     /locus_tag="ACHBFA_002891"
     CDS             complement(1150..2028)
                     /locus_tag="ACHBFA_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM9396295.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          687 a          381 c          363 g          746 t
ORIGIN      
        1 tttttcggcg ttacttttta aagttaactt tttcgacaaa tagaaagaga tacgaatcac
       61 tgattttttt gaagaaaaga aatggagtga gctgaacgtt agagatattg ggagctttcg
      121 taactgctta ttaaacgata atggttattt gcaaccgtat tgctcgcttc tacatgaaat
      181 atttcatgaa atatttcgca aaacctttga ttatgtagtt acgtttccat tcttgactaa
      241 aaatgtttca aaaacgaaca aaggtgttaa aaggtaaagt gatcaatact actggtcaaa
      301 ataagaggtt gaaagggaat tgaaaaataa ctacagataa tacttttata aacgtacgtg
      361 ctttgtggtt ctgtagttaa ctatataact ggaattaggg aatggtatca atttgaaaag
      421 aaagattcta ccaatttatc atatgttaaa aatggaaaat caacagcagt tctttcggaa
      481 accttacaca aaaaccgatt attgaaaata gccgaaaaac gtttttcggc tattttttta
      541 attaagtttc cttcttctta ccttttccca ctattccgtt tcacatcttt gatgatcttc
      601 agcgcacgtt ttcgtgtttt gatatttggg cttttcaagc ggcggtatgc acttgagata
      661 tctgtttttt ccatatggac gacccacttt cttctttgat tgtagtcatg ctaaaaaaca
      721 caaatcgaaa caattacgat ttgtgtttta aatggtatca aaagttaaaa tgaaagtcaa
      781 cttgttaaaa cagtcgatca gactagctga ttgactccta tctctggaaa taaattttca
      841 ttattacgtt ttagctacct aaaaaaggcc ctcttgtccg ttaactttaa tatctattgt
      901 tatagctagt ttacctattg aacgtactgt gtgttcgata gaacactaaa aaagaggctg
      961 ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt
     1021 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt
     1081 tagagcgtga gacaaaagtg ttctttactt ttgtctcacg ctcttttaaa ctatttcgaa
     1141 gtactaaact tatgaagtaa gaatacctaa gccagagcca atgtgctcgc ctgcaagcaa
     1201 ttgctctttt gcttgtcgct gtaaactcgt actttctgga taatcatgta tttgggcatc
     1261 tactcgtcca aagatccaag ggactccact atcgtcctgt tcatcaactg ataataatcg
     1321 ataatcggct gcaaaaagac agatttttga ctcttttttc aaccatttag taagattggg
     1381 tgatagtaac caagtatctg tgtacatcac ttggtaatgg taatcaggaa aataaagttc
     1441 aaaaaaacga atggcacttt cgtaactatc tcttatctta ttctgagata aatctgcatc
     1501 ggaaggaata tgaagagaaa ggcactgctt tccttctaat cttagatccg tgtacccttt
     1561 cggaaaatat gtcatttcgt actccaattc accaatccga aagatcaagc cactagtgta
     1621 atgccaaatc caaaaaccac ggtcaaagcg atagcttcct gttacttgga aatgttcatg
     1681 aataaatcta gtaaaagcat tcattgttgc caaatagata gttcttggaa tcttcaatgg
     1741 ttgataaatt tttttccaac tttgagaagc tgcatataga taaatagcta atgctgcaaa
     1801 aggttcctct tcatagactt tgtctatttt ctttgcagtc gcctttgctt tattaggatc
     1861 cattagttca tgactatatt gctcaaacac ctcatcaagg atgttttttg cttgtacaat
     1921 catttgctct ataacgactt tgggaagagt taaagtttct gccagaattt ggatttcatt
     1981 gcttattttc ttcatcaaat ttcccctttt catatctttg aacctcattg tttaaatgac
     2041 tgattatccg gacaccatat ggtataaaca tacttttatt gtacactaac tataaaactt
     2101 aaactttcta tgaagaaaga atatctgtaa gactttcaag gaaagcattg accatctcag
     2161 gaaagtttat tcagtta
//
LOCUS       DAYYNG010000250         2167 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000250 DAYYNG010000000
VERSION     DAYYNG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.256"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1200
                     /locus_tag="ACHBFA_002892"
     CDS             115..1200
                     /locus_tag="ACHBFA_002892"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9396296.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            1336..>2167
                     /locus_tag="ACHBFA_002893"
     CDS             1336..>2167
                     /locus_tag="ACHBFA_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9396297.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     S"
BASE COUNT          782 a          236 c          402 g          747 t
ORIGIN      
        1 gaaaacacct aaacaaaaat ttgtttatag attaaaatca acaattggac aaaaaaaatt
       61 taacaggtta cttgcactga aaaaattttt acacttataa aggagaaaag tactttgaag
      121 aaaatttgtt tcattagtgg tgtaatttct cgttctgggg gaactgaaag agtagggagt
      181 attattgcca atagcttgtc tgatttaggt tatgatgtca ctattttaag cttttggaat
      241 agtggagagc cttattttgg gttagataag acgattaagg ttgattatat gttaaatcct
      301 aaaacggagg gaaaattata tagaacttat ttatatccgg tttggaaatt gcacaagtat
      361 atatgcaaaa atgaatttga tatagtaatt gatattgata ctttgttgtc tttgtatact
      421 acaagggcaa ttaaaggaac aaagtgcaaa ttgatttctt gggaacattt taattactgg
      481 acaatggaaa tgctgaaaga aaagaagaga ttcaaggcta aaaaattgat aaaaaaatat
      541 gcaaagaggc ttgtggttct cacagaagaa gatcgtcaaa aacatattga tgaatatagt
      601 ttatcagaag attttgttat aactatgcca aatccgtgta tatctaatat cgagtacgaa
      661 tatcattatg ataaaaagag atttcttgca gtagggcgat tagtggaaga gaaaaatttt
      721 gctgacatga taagtgcttg gaaaattgta gaaaaaaaag tagatgattg ggatcttatc
      781 attgtaggaa aaggtgaatt agaaaaagat cttcgccagc agataaaaga tttaaatctg
      841 caaagagtaa ctatagcagg gcattcagac catgtggagg aatattataa atctgcatct
      901 tgttatgtgc ttagttctgt ttatgaagga tttcctatgg ttatattaga agcacagtct
      961 tatggattac cagttatttc ttatgattgt aaaactggtc cgagagattt ggtacatcat
     1021 aattttaatg ggatgttggt tgaagataag aatatagacc aattagctaa gagcatgata
     1081 atgtttacta agaatactga tttagcaatg agaatgtcac tgaacgcata taataatgta
     1141 caaaaattta atttgaaaga aattacaaaa caatgggttg ctttgattga aaatgtatag
     1201 ttttttgtgg ttgtccaaac atgaaaatta gcgtaattaa tcaaagagct agaagaaacc
     1261 tattacattt caagatttta gaaattatat agtattaggt aacaataaaa aatagaaagt
     1321 ttggagccat atgatatgat aattaatcgt aaacaaaatg ctaaaaggaa tataattact
     1381 ggaacaattt ttaagttata tcagattatc attccctttt ttatgcgtac acttatgatt
     1441 tatcagatgg gaatggagta tgctggattg aacaacctgt tcacatctat atttcaaatg
     1501 ttaaatttag cggaattggg aataggtgct gcactaactg taagtatgta taaacctata
     1561 gcagaagaca atagaaaaga gatttgtgct ttactgaacg catataaaaa gtacttttca
     1621 attattggat ggatagtgtt tacattagga ttgttatgca ttcctttttt gagattttta
     1681 gttaaaggtt cgattcctgg aaatttaaat atgacagttt tgtatttaat gtatttgata
     1741 aatactgttt tgtcgtattg gttattttca tatagaaaga gtctaatata tgcacatcaa
     1801 agaaatgatg taattaacgt gtctatgcta attactttta cattccaatt tgtaatgcag
     1861 gcatatgttc tgattttctt aaaaaattac tatttgtatt tattggcttc tattttggga
     1921 caaatagttc ttaacttaat gtgtgctgtt gtttctcaaa agatgtatcc tgattatact
     1981 cctgttggtg agttagagaa agaaactatt gacgatatta taaaaaaagt aaaaggatta
     2041 gttacaaaca aggtaggcgg tacaatatta cgatcttcag attcaattat tatttcatct
     2101 ttcttaggtc tttcagtact tgcgatttat cagaattatt actttattat ttctgccata
     2161 atgagta
//
LOCUS       DAYYNG010000251         2106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000251 DAYYNG010000000
VERSION     DAYYNG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.257"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACHBFA_002894"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACHBFA_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACHBFA_002895"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACHBFA_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACHBFA_002896"
     CDS             1898..2059
                     /locus_tag="ACHBFA_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396298.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          754 a          330 c          361 g          661 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatga
//
LOCUS       DAYYNG010000252         2090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000252 DAYYNG010000000
VERSION     DAYYNG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.258"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2090
                     /gene="acm"
                     /locus_tag="ACHBFA_002897"
     CDS             <1..>2090
                     /gene="acm"
                     /locus_tag="ACHBFA_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM9396299.1"
                     /translation="SMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGN
                     IKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSA
                     QVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGS
                     SSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQ
                     IHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTII
                     ENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYIND
                     TEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWID
                     FDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRAS
                     TNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQK
                     IENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDD
                     WRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITP
                     WKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKT
                     SSKKSSFAVIIGLL"
BASE COUNT          819 a          343 c          406 g          522 t
ORIGIN      
        1 atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg tctatgcgga
       61 tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa cgaatattac
      121 agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa atatccaacc
      181 aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg aaggattcca
      241 aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg tcagaaaaga
      301 ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag aaggttgggg
      361 gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata cgggaccatt
      421 caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag cttctggttc
      481 ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat
      541 tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag
      601 tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca
      661 cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt
      721 ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa
      781 aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga
      841 acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt
      901 tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg
      961 tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc
     1021 aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat
     1081 cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta
     1141 tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga
     1201 actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa
     1261 gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc
     1321 gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc
     1381 agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta
     1441 tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga
     1501 ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa
     1561 agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa
     1621 acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga
     1681 agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg
     1741 gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga
     1801 cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag
     1861 ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct
     1921 attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa
     1981 ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc
     2041 taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga
//
LOCUS       DAYYNG010000253         2056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000253 DAYYNG010000000
VERSION     DAYYNG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.259"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            493..714
                     /locus_tag="ACHBFA_002898"
     CDS             493..714
                     /locus_tag="ACHBFA_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396300.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="ACHBFA_002899"
     CDS             715..972
                     /locus_tag="ACHBFA_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396301.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACHBFA_002900"
     CDS             complement(1108..1710)
                     /locus_tag="ACHBFA_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9396302.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACHBFA_002901"
     CDS             complement(1777..1932)
                     /locus_tag="ACHBFA_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM9396303.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAYYNG010000254         2020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000254 DAYYNG010000000
VERSION     DAYYNG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.260"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..751
                     /locus_tag="ACHBFA_002902"
     CDS             <1..751
                     /locus_tag="ACHBFA_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9396304.1"
                     /translation="IFKYCQRKNLISENPMKSVRMPKYQLTVTDLKEAINNIEDKFLT
                     VDELRTFLNYGIVHEELPMSVLFHVLFYTGCRVSEALALQPGDIDFKRNEILFYKQTA
                     VKGKSKDFRIETTKTVSSARRVLVTPLVMEKLQQLIKALGEMRQHYRFIIEETYLFVY
                     LDSSKRGVPYRREYVNDHVKRCVNRSGINKPFHTHLARHTMASLVAEHCSWEVLKDRL
                     GHTDQTTSKIYRHLTSNEKVKPLIAFSSLES"
     gene            complement(932..1117)
                     /locus_tag="ACHBFA_002903"
     CDS             complement(932..1117)
                     /locus_tag="ACHBFA_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396305.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            1154..1447
                     /locus_tag="ACHBFA_002904"
     CDS             1154..1447
                     /locus_tag="ACHBFA_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM9396306.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1535..1681)
                     /locus_tag="ACHBFA_002905"
                     /pseudo
     CDS             complement(1535..1681)
                     /locus_tag="ACHBFA_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          726 a          300 c          374 g          620 t
ORIGIN      
        1 gatttttaaa tattgtcaaa gaaagaattt gataagtgaa aatccaatga agtctgtgag
       61 aatgccaaaa tatcagttaa cggtaactga tttaaaagaa gccataaata atattgagga
      121 caagtttctt actgtcgatg aattgagaac ttttttgaat tatggcattg ttcatgagga
      181 attaccgatg tctgtacttt tccatgttct attttacact ggctgccgag tcagcgaggc
      241 gcttgctcta cagcctggag atatagattt caaaagaaat gaaattctgt tctataagca
      301 aacggcagtt aaaggtaaga gtaaggattt tcgaattgaa actactaaga cagttagttc
      361 tgctagacga gttctagtaa ctcctctagt tatggaaaag ctacaacagt tgattaaggc
      421 tttgggtgag atgaggcaac attacaggtt tattatagaa gaaacatatt tgtttgtgta
      481 tcttgattct agtaaacgtg gtgttcctta tcgtcgagag tatgtaaatg atcacgtcaa
      541 acggtgcgtg aatcgctcag gaattaataa gccatttcat actcatttag ctcgtcatac
      601 aatggcaagc ttagtagcgg aacactgtag ctgggaagtc ttgaaagatc gcttaggcca
      661 tactgaccaa acaacttcga aaatttatcg ccacttaacg tcaaatgaaa aggtaaaacc
      721 tctgattgct tttagttctt tagagagtta aaaaaacggt gaaagcactg gtattacagg
      781 attcttgaaa aacgattttt taacaaatta gtcaatatgt agtcagaggt aataaaaatc
      841 cttttatatc aatactttaa cgacttttca caagtatcaa agaaatccgt cgttaatcat
      901 tgttatcaga ctgagaaaga acccttggta ttcaagggtt cttttttctt agcatgtaat
      961 ttgaacaccc tgatccagtt ttttctttaa ttattggtag gggagaataa tggattagaa
     1021 taaatctacc tataagtagt agtaaaaaaa agagcactat gataatatct ttcttaagag
     1081 taaccgcttt aataaaaaat ccagaatatt ctaacataaa tggagtattc ttagaatcaa
     1141 aaggagaaag aagatggttt atacttatac ggagcaagaa aagatgaaag ttttagacac
     1201 attcatgaat gatggaaaag taaaacaagt accaagttca acaaaaaaga aatacatcct
     1261 attgaaagaa gtcttgaaac gctttgacca tggagtgact tatacagaaa cagaggttaa
     1321 ttcgatactg ctgaacgtat tttctagtgg cgattatgtc gaacaaagac gttatctaat
     1381 cacatttggc tttttcaaaa gaagttcaga cggtcgggca tatcagatga tgggaattga
     1441 aaattagaag ttttaattta tactcattga aaaaagttca agtatgcgat attggttcta
     1501 caccgcatac ttgaactttt tttgtcatct gtatctataa tacaaagcca agaacgttat
     1561 tcaaaatatg gatagctatg ataaaagcta atgagcgatt cgcagttaaa taagcaatcg
     1621 tcagtaaaat cgttccagga gcgtacatga aaaaatcaat tggatgcgtt acatgaataa
     1681 agataattct cttttatttg tacgaataaa taccaaaacg tacctcctaa aattagattt
     1741 tggacagatg ccctttaaaa agataacaag atttcgttct tgaaacaata tatatgcttt
     1801 tattccaatg aggttgtgaa aacagtattt tgtcctgagc gttagaaaat tgaaaatggg
     1861 gaatagcaac tcgtacttta ccatttttta agtgaataca agatcagcta gttgaacacc
     1921 gtttattcgt gatgagggac acaacactgc agtcgacaca tttctctttt atgttcaaag
     1981 ataacaaata tttaaaacaa agaaaaatag tagataatcc
//
LOCUS       DAYYNG010000255         2018 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000255 DAYYNG010000000
VERSION     DAYYNG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.261"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            632..964
                     /gene="traE"
                     /locus_tag="ACHBFA_002906"
     CDS             632..964
                     /gene="traE"
                     /locus_tag="ACHBFA_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM9396307.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1583..1924
                     /locus_tag="ACHBFA_002907"
     CDS             1583..1924
                     /locus_tag="ACHBFA_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM9396308.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          700 a          270 c          428 g          620 t
ORIGIN      
        1 atatagaaaa gggaacgtat gtttgcatac tccttttttt tgagttataa taatacgcga
       61 agagagataa tttcattatt taaacaagct aaaaatcttg atattacttc ttttcaggca
      121 atacgaatat tattcgctta cattttttgt ataaatttaa ttgtctgcta tgcttgtgat
      181 gaattgattt tcgttttgca cttttggatg ataaatttgg acaattgaaa atacaaaaaa
      241 aagaaaggcg gtatgaactc ggaaggctgg taaccttcgt cagcgttcat acacggcatt
      301 aagatttggc ccttaatgcg ttaacctttc agtgttacgg tattacccgc aatggattta
      361 gtataacaga tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg
      421 tgatattacg atgaatctat gcctaagcat aatgttataa aaatgtccgt gtggctcttt
      481 gccgtatgac gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt
      541 gtttgtcatg aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttgttaa
      601 aaatatttaa agaaattggg tggtttattt tatgtcatat acaggaattt tgagtctcaa
      661 agatatttgt cactacggta aacggtgtac cgctacagag aaaatcacga agaaactatc
      721 aactggccaa agtaaaaccg ttgtgcagtg taagaaatat ataatccaga aagacaaagt
      781 ctcagaggaa atgatttatt acattgggaa acaaaagcaa ataattttaa aagatcctat
      841 ccccttaaaa gagctatatc caacaatcaa acatgtttat gaccaaaacg gtgtactgat
      901 tggtcgaaga aaaaatggag tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg
      961 ctgaattgat cattctcata acttgtattt tatcgttttt cgcttgcttg aaaatattgt
     1021 cgagacttct aaaggaactt agagtcacta gaaaaataag gagtagactt gatctatcaa
     1081 caattccctt cggggagtga cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc
     1141 aatcgagtaa atgcgcttgt ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc
     1201 agagtaaaca agagggaatt tagaagttag acaagttaga atagcgtcta gattaatcat
     1261 gagcagctac taagggatct agactttgac gcaaactcat ggaataagga gtaggtcttc
     1321 tcgtaatggg gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg tagatacttg
     1381 cgaggacgtt gattattgga tcaatcgagc atttaccgct gtctactagg gtttctcgtt
     1441 tcggacgcca taaacgagtc gaaatttttt atgtattttt aagtaaacga aaaacgataa
     1501 aatatgaaca agtcctgaat aaagtgaaaa aattaatata aagcatagag aaaatgaaat
     1561 ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg aatatacgat tgggcacaca
     1621 tactattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagggg
     1681 cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat acgatcaaac
     1741 attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag gtaatgaatg
     1801 gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag atacttggtc
     1861 atatttgagt gattatattg tcagctttga aatcattgaa tttgtagcgg ataaagaaga
     1921 ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac gacgattaat
     1981 aaaaaggtag tattacctta ggcattgagt aaggaatt
//
LOCUS       DAYYNG010000256         2001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000256 DAYYNG010000000
VERSION     DAYYNG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.262"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..376
                     /locus_tag="ACHBFA_002908"
     CDS             <1..376
                     /locus_tag="ACHBFA_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM9396309.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /locus_tag="ACHBFA_002909"
     CDS             424..1824
                     /locus_tag="ACHBFA_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9396310.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DAYYNG010000257         1979 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000257 DAYYNG010000000
VERSION     DAYYNG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.263"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACHBFA_002910"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACHBFA_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACHBFA_002911"
     CDS             complement(728..1744)
                     /locus_tag="ACHBFA_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9396311.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAYYNG010000258         1958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000258 DAYYNG010000000
VERSION     DAYYNG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.264"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1458)
                     /locus_tag="ACHBFA_002912"
     CDS             complement(67..1458)
                     /locus_tag="ACHBFA_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9396312.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            1584..>1958
                     /gene="tagD"
                     /locus_tag="ACHBFA_002913"
     CDS             1584..>1958
                     /gene="tagD"
                     /locus_tag="ACHBFA_002913"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFM9396313.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQI"
BASE COUNT          739 a          320 c          273 g          626 t
ORIGIN      
        1 ctataaaatt tgttgaagtt gggataacta gttttctttt ctactttcat atacctaaaa
       61 atagtttcaa ttagttactc cttcttcttt agatatagtt ttttggatag aacccagata
      121 tccccaaaaa ataaatgaac ctatcgtact aaccaaaatg agttctgtga taaataatgc
      181 cgaaactgct attgtaagaa tcattaaaat agcaaaaaag tgcttatctt ttactagatt
      241 tggttgtgtg aaacaataac gcaaggcttt aaagcattgg ataaataaga aggttaacaa
      301 tgtcaataga cctaatcctc ctgtagcagc catcactaag aaaataaaat tatgagcagt
      361 taaagtatct tttcccatta atgtatatgg tagtttatca tgtgcatatt caatatagta
      421 gcctggagaa cctccggata tcggcttact cttgaatatt tcaattgcac ttttccaaag
      481 ttcaaacctt aaattagaaa catccccgct atcagtgtca gggcgtttta aatcaacttt
      541 attgctgcta gttgttcctg atgaatcaga tgtatctttt tttacttctg taatatgcgg
      601 aacatgtgcc catgcatgag cagttatatc tgttacataa tacatcgcaa ttatacttag
      661 caatcctact actaatgcag ttagccatct ttttaaagta ttagttttaa gaaaacgact
      721 actataactt gtaaagaaac taaaaacaaa agttgttatt aataaacaaa ctaatgtggt
      781 gcgtgaaccc gataaagcaa taaacgagaa ttgaataaaa atattaatta caaaaaatat
      841 tttaaataga agatttaaat gcttgtaagt gaccaaataa tatatagcaa taatgatgac
      901 gattaatgat ataaccgcac caaaatttgg atctgtatat acaccaaata aacggttgcc
      961 taaccaccct aaacgaactg ggtaatactt atattttatt ggaattttta tgccatattg
     1021 aatgacaaac cagtataatg atactaaatt agctaaaaac cataccaatg aaagtatcca
     1081 ggtaaatact ttgaaaaaaa agttactact atagtctttg ccaaactgat aaatcataaa
     1141 gaaaaagatt aattgccaca tgattaactt aatattttcg ctatatccat attttaaatt
     1201 cattaaactg cttactagca tcgcgccaat aaaagcaatt aatagccaat catatttttt
     1261 tagatttttt tctctaaatg caataattgc atctataatg atcaagacaa ctcccgctat
     1321 tccagcaggc caaaaaagta aagtatcata tctagtaggt aatagtaaat acgggcctat
     1381 tgctcgtaac attaaaataa aaccaaaata aataaaatac caacctttca catattttaa
     1441 aaatctttca ttcaacattt ttcctccatc cacctatttc tcataaatat ttcttatcct
     1501 ttttaggata aactggtcct ttcaattcta acaaaaaaat gctaaactaa caatgtatat
     1561 taatgaattt cgagaggtga aagatgaaaa gagtaattac ttacggtacc tttgatttgc
     1621 ttcattacgg gcatatcaat ttactgaaac gagcaaaaca atacggagac tatcttattg
     1681 ttgccctttc tacagatgaa ttcaattgga atgcgaaaca aaaaaaatgt tattttagtt
     1741 atgaaaaaag aaaacagtta cttgaagcta ttcgctacgt tgatttagtt attcctgaag
     1801 aaaattggga acaaaaagta aaagatataa aagaatacca tgcagattat ttcgtgatgg
     1861 gcgatgattg ggctggacat tttgatgaac ttgaacaact aacagatgca caagtgattt
     1921 atttaccaag aacacctgag atctctacaa ctcaaata
//
LOCUS       DAYYNG010000259         1942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000259 DAYYNG010000000
VERSION     DAYYNG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.265"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="ACHBFA_002914"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="ACHBFA_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9396314.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="ACHBFA_002915"
     CDS             851..1039
                     /locus_tag="ACHBFA_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9396315.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="ACHBFA_002916"
     CDS             1052..1609
                     /locus_tag="ACHBFA_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9396316.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="ACHBFA_002917"
     CDS             1641..1883
                     /locus_tag="ACHBFA_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9396317.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          714 a          301 c          400 g          527 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac ac
//
LOCUS       DAYYNG010000260         1928 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000260 DAYYNG010000000
VERSION     DAYYNG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.266"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACHBFA_002918"
     CDS             <1..367
                     /locus_tag="ACHBFA_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9396318.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACHBFA_002919"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACHBFA_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1928
                     /locus_tag="ACHBFA_002920"
     CDS             1330..>1928
                     /locus_tag="ACHBFA_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9396319.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          627 a          290 c          453 g          558 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaaaaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaata gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactat attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattggc
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DAYYNG010000261         1906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000261 DAYYNG010000000
VERSION     DAYYNG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.267"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1906
                     /locus_tag="ACHBFA_002921"
     CDS             <1..>1906
                     /locus_tag="ACHBFA_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9396320.1"
                     /translation="VLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQLFSDGKLL
                     KNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSG
                     VGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEK
                     DTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILA
                     TTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIFCSIE
                     LFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEISIDL
                     KKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIALRNY
                     VVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQKL
                     LANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETNK
                     LLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESNV
                     IGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNVIVS
                     CLSGEGISEKV"
BASE COUNT          688 a          240 c          349 g          629 t
ORIGIN      
        1 gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca agatattgca
       61 atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt attttcagat
      121 ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa tcaacaaaag
      181 ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc cactatagat
      241 aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat aaaaggaaac
      301 agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct tgatagaaaa
      361 gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc aaataatcca
      421 gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg ggctgaaaaa
      481 gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga tgaggttcac
      541 aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca aaagtatcgt
      601 aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt agccacaaca
      661 gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc tgaaatcact
      721 ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt tgaatttctt
      781 aaagatgaag caattacaat tgattcaaaa atattttgtt cgattgaact ttttactgat
      841 cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat caagcttctt
      901 tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg ggacaaacta
      961 gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat gtttcttaaa
     1021 tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat tgaagaaagc
     1081 atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga gtataattat
     1141 ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat atcaccaatt
     1201 attgattcga caggtctatg tttattagat gaattcttgg cggatatcag tctatttata
     1261 atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa gttcagaaaa
     1321 tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag tagtttagaa
     1381 gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt aaagaagttc
     1441 tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat aacagcttct
     1501 gcgatcgcaa gggaaacaaa taagttgctg aatacatatg tttatgaggc tttcgatatg
     1561 ccaattgaag gtgagactag tgacttaatt aacctaatca acaatttttg taaaagtata
     1621 gatacgacga atggactcat tctgctagtt gatatgggat cattggaaca aatgtatgaa
     1681 aaaattgaat ccaatgtaat aggtgatttg gtaattttaa acaatgtttc tacagcttta
     1741 gcgattgaat gtgggattca agtttgccaa aaaaagccaa ttagtcattt ttaccagatg
     1801 gattttgatt cgtttcaagt taaagttcag tattataaag gattatcaca aaagaaaaat
     1861 gtaatagtat cttgtctctc tggtgagggg atttctgaaa aagtta
//
LOCUS       DAYYNG010000262         1880 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000262 DAYYNG010000000
VERSION     DAYYNG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.268"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..336)
                     /locus_tag="ACHBFA_002922"
     CDS             complement(142..336)
                     /locus_tag="ACHBFA_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396321.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
     gene            584..1725
                     /locus_tag="ACHBFA_002923"
     CDS             join(584..812,812..1725)
                     /locus_tag="ACHBFA_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9396322.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          626 a          394 c          351 g          509 t
ORIGIN      
        1 aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga
       61 tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca
      121 tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat cgacgaacgt
      181 cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg ccgctttgtc
      241 tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat tgtctctgct
      301 tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc ttctctttct
      361 tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa caattcattg
      421 aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat tcacttaaca
      481 actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat ttcatggaca
      541 cgatttggta gaatctaact actaagaaaa ggacgtgtcc acgatggcaa aagccaagaa
      601 aaaatttgat gaagacttta agaagatgat cttagattta aaccagtctg gccaatcggt
      661 cgaagaactg gcagcgcaat atggtatcgc tacacaaacg atttatcgtt ggaagaagct
      721 tcatacaaag aatgtagcaa ccggcatgac tgaggcagaa atcctagcga tgaaaaagga
      781 aatggcccgc atgcaggagg aaaacactat cctaaaaaag gctttaacca tattcgctca
      841 aaagtaaaaa tgagtgatgt cttcgaattt attcaatcaa atgttcatga tcatgacgta
      901 aaacaaatgt gtaccgtttt ggaagcaccg aaatccagct actatgactg gaagaaaaag
      961 aaaccttcca agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc aggaatctac
     1021 tttgaaaaca agggtatcta tggtgcgcct aaaattcata agattcttgt tggccgtggc
     1081 gagaccttgt ccttaaagaa agtacaaata ctgatgagaa aattaggctt gcgttcagtc
     1141 accctcaaga agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg acgtaaaaac
     1201 ctgttaaacc aagattttac cactacgtcg atcaatcaaa aatgggtgac agatattacc
     1261 tatattcata ccctaaaaga tggctggacc tacctctcca ctattcaaga cctccacaca
     1321 aagaaagtca tcggttggaa gtttggcaag cagatgacga aagaattggt tatcgaaacc
     1381 cttgacaatg cgctcttaaa ccaaacaccc tccgagaact tgatcatcca ctcagatttg
     1441 ggttctcagt acactagcga agcctatgag gcaaagctga atgaattgaa cattcggcac
     1501 tccttcagtc gtaagggctg tccctacgat aatgccggta tcgaatcgtt tcacgcctca
     1561 ctcaaaaaag aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc tgcttattta
     1621 gcgttatttc aatacattga aggattttac aatcgcaagc gaatccatag tagtatcaac
     1681 tatttaacac caaatcagat ggaggagttg gcattgcaag cttagttcct agctaccact
     1741 tacatttttt tggcgagatt tccacagggt ttcaacttca tcgcttcgat cagcggtcaa
     1801 acggttttgg tttgaccgct ggtcgaaacg atgagatact cacccgctgg aatctcgaaa
     1861 aaattctcac aaatcgtgtc
//
LOCUS       DAYYNG010000263         1828 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000263 DAYYNG010000000
VERSION     DAYYNG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.269"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            342..1724
                     /locus_tag="ACHBFA_002924"
                     /pseudo
     CDS             342..1724
                     /locus_tag="ACHBFA_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          684 a          314 c          328 g          502 t
ORIGIN      
        1 aaatgatatc cgactcctct tcatacgttc tggactattt gctaatagat taaaatttca
       61 gcatctattt ctgaccataa ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa
      121 ataaaggatc aaaaactttt aagtttctga ccccgtaact cacacttcaa aatcttactt
      181 aaaaaggagt ctgaaagaaa aaaagagtat tcaaaatttt tttctaacta gctacatcac
      241 atctatgcat tgacactcaa agaaccagaa taagtcatca gaaagcaagg gggtgcggac
      301 ataatcctgg acgaaaaaat aaacttagga ggaatgtccg tgtgttttcc cacaaagagc
      361 gtgtaagggc aattcagtta tttttaaaat acgattgctc ttatgctgca accattcgag
      421 aattagggta tccgtcgatt ggtgcactac ggaaatggta taaagagtat ttggaatccg
      481 gagagctcca tcaggagcac cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag
      541 cggtgaatca ttattttgaa tatggtcaat gttatgcacg aaccattcgc atgcttggtt
      601 atcctaatag agagtccttg cggcagcggt gtgaagaact tgcaccagga gctagaaaac
      661 tccgaaaaag tgcggtaaag ttaacacaag atcaaaaaga agctgtgttg aaaaaattct
      721 ataaaccgca aactaatagg aaaaatttag ccgaatctgg aggaattagt agagttactc
      781 tatatcaatg gaaaaacacg gttcttggaa aggattttcc attgtgtatg actttaaaaa
      841 atcaggaaaa acaaaaagag ctacttttaa aggaagtaga agacttacaa aaacaagtac
      901 accaattaca gctcgaaaaa gcattactcg aaggagcggc tgaactgcta aaaaaagaaa
      961 aaggcgtcaa tcttctttgt ctatcaaatc aagaaaaaac gatattgatt gatgccttac
     1021 gaaatcagtt caccttgaaa gagcttcttc aacagctcca gtttcctaaa agcagctatt
     1081 tttatcaaaa acaagcactt gagaaaccag ataagtatta caaagaacgt caaattaatt
     1141 atcacgattt tcaatcataa cttctgtgcg tatggttacc gtagaattca ccaagcttta
     1201 aaaaatatgg gtaaaaagct ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat
     1261 ctatttgtta aattttctag acgaaaaaag tattcttcgt atgctgggga aatttctcct
     1321 gcacacccca atttattaaa tcggaatttt aaagcaaaga agccaaatga aaaatggtta
     1381 acagatatta ctgaatttag aattctcgca gggaaaatct acttatcacc acttgtcgat
     1441 tgttacgatg gtgctattgt tagttggact attggaacaa agccaacttg cttcactcta
     1501 tgtctaaaaa gagttattca ccagataatt cagcgtgcga aggtttcttt ggacgattaa
     1561 aaaacgagat gttttatcaa cgtgactgga agaatacaac aatcaatcag tttataaaca
     1621 aattggatga ttatattcat tggtataata atcaacgaat taagttatct ttaggaaggc
     1681 taagccctct tcaatatcgg aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa
     1741 tatccgcacc cccttttttt tgtaaaaaac tattgcacaa tacaagtagc ctacggctat
     1801 cgaaatttcc ataactttcg agcaagaa
//
LOCUS       DAYYNG010000264         1804 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000264 DAYYNG010000000
VERSION     DAYYNG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.270"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..238
                     /locus_tag="ACHBFA_002925"
     CDS             <1..238
                     /locus_tag="ACHBFA_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9396323.1"
                     /translation="MILIKGPKEAFELVQNLPEIKEINFGGVSKKSESKQYGKAVFLT
                     PEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            252..1001
                     /locus_tag="ACHBFA_002926"
     CDS             252..1001
                     /locus_tag="ACHBFA_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9396324.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1001..>1804
                     /locus_tag="ACHBFA_002927"
     CDS             1001..>1804
                     /locus_tag="ACHBFA_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9396325.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGF"
BASE COUNT          564 a          251 c          361 g          628 t
ORIGIN      
        1 catgatttta attaaaggac ctaaggaagc ttttgaatta gttcaaaatt taccagaaat
       61 taaagaaatt aattttggcg gtgtgtctaa gaaaagcgag tccaaacaat atggaaaggc
      121 agtcttttta actcctgaag aattagaagc cacaaaaaaa attcttgatc aagaagtgaa
      181 aatatttgtg caacaagtcc catcttcaag tattgaaaat gtagatttta gtaagtaagg
      241 ggggaaaata aatgttatta aaagccattt tattaggttt agttggtgtg gtagctgtta
      301 ttgactccag gttaattggt cgacaaaata ttggtagacc actaattttg agtacacttg
      361 ccggacttgt gctaggtgac ttgcatacag ggattgttct tggggcatct ttagaattaa
      421 tttctatggg attcgtctca attggggcgg ctggacctcc taatatgcaa cttggtagta
      481 ttattgccac tgcttttgca attttaacta atagtagtac agaaacagcc ttaacaatag
      541 ctatcccagt ggctgtagct ggtgaattta tgagtattat catgcgtatg ataattgctc
      601 aatttggaca tgctgcagat aaagccattg aaaataataa atttagaaaa gctcaaatta
      661 ttcatattta ttggtcattt atttttaatg cttttgttta ctttattcca atctttttaa
      721 cagtatattt tggtgctgac gttgtggcag atttagtaga taaaattcca gaaatgatta
      781 cagacgcctt gactgtctct ggaaatatgc tttccgcatt agggtttgca atgctattaa
      841 gcacaatgtt gagtaaaaaa ttatatcctt actttatttt tggattcttt attgttgcct
      901 attcaggtct ttcactaatt ggcgtgacaa tttttgcagc tctgattgcc tttgtaatgg
      961 atcaagttaa atatggaaaa agagaagagg cgcatggtta atggaaaata gtacagaaaa
     1021 aaaacgttta atcacaaaaa aagatcttca taagatgtgt cttcgttcat ggacaatcat
     1081 gtgttcgtgg aattatgaaa gacaaatgca tatgggattt atgtatggga tggcgccaat
     1141 tttaaataaa gtttataaaa atgatgaaga gaaaaaaaga gaagcttata ctcgtcatat
     1201 ggcatttttt aactgtacgc ctcaaatgac tccatttatt atgggacttt gtgcttctat
     1261 ggaagaacaa aatgctcaag cagaagaagg aaactttaat actgaatcta tctccatgat
     1321 taaaaccagt ttaatgggtc cttttgctgg aattggtgat tcatttttcc aaggtacttt
     1381 aagaattatt acatttggga ttggattgtc ttttgctaag caaggtagta ttttaggacc
     1441 gattttagct gttggccttt ttgctatacc ttctatttta gctgtttttt acggcggtaa
     1501 attaggatat gagtctggca ataaatacct tactaaattg tatcaagaag gtcttatgga
     1561 tcgagtaatg caatttgctt ctatcattgg tttggctgta gtaggtggaa tggtggctag
     1621 catggtgcca attgtcaccc ctttaaaatt cactacaggg ggaacagaac ttgttttaca
     1681 agacttactt gattccatta ttcctggatt attaccactt gtatgtactt tgggaatata
     1741 taaattgatt aagaaaaatg ttagtacaaa tatgctctta ttaggttgta ttttggcagg
     1801 gttt
//
LOCUS       DAYYNG010000265         1789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000265 DAYYNG010000000
VERSION     DAYYNG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.271"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..200)
                     /locus_tag="ACHBFA_002928"
     CDS             complement(<1..200)
                     /locus_tag="ACHBFA_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9396326.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKG"
     gene            complement(250..711)
                     /locus_tag="ACHBFA_002929"
     CDS             complement(250..711)
                     /locus_tag="ACHBFA_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9396327.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            complement(693..1667)
                     /locus_tag="ACHBFA_002930"
     CDS             complement(693..1667)
                     /locus_tag="ACHBFA_002930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9396328.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          583 a          333 c          228 g          645 t
ORIGIN      
        1 atccctttaa catcactaat tttaacaatg tctaatttgc aatcagcagg tttagcaatt
       61 gtcaaagcca tcacagacat tggatctcct ggaactttgt tatcagccac aataatatgg
      121 ttaatatcat actgtttagt ccaagaaaat attacttgtc catgaagtaa tctgtggtct
      181 acacgcacta atttaatcat tctatttcct ccttcaattt ttaaaattta tagaatagaa
      241 attgccacct taactgacat cttttaaaag cgaattaata tgaaccatac ttttttgaga
      301 gttattcagt aattccttaa aatctatttc ttctatagat ttaccagctg actgggccat
      361 taataactcc agtaaaagag gtaaattaat cccagcaata atttgaattc tattgttgtt
      421 caactgttta atagaaacat tattaggtgt tcctccaatt aaatccgtta aaataattag
      481 aggttgcaaa tgatgctgac taattatttc tttcatactc tcctcaaaat catctggact
      541 agtattttct ttaaatggaa cagcactaat acctgtggcg tcattcatta aaattaattt
      601 tgttgccttt aaataaccct cacaaagggt agaatgtgtt gtaatcacaa tgtgaaataa
      661 ttgatttgcc atcttttgca acctcctatt aattaatatt tcatttgcca catatagcgt
      721 ctaacatcta aggaatggcc tctttcaaaa gcaatcgctt ctaccatttg gcgaataaca
      781 acgccactaa gtacagctgc aatatattct ttcgctacac tatctataga ttctaagtca
      841 aaatcagctt ggtctaaaac cattaattta ttactgaatt ttttagtgaa gtcttctacc
      901 cgttgatcta aaaatcttgt atctccaata cttttcacta agatgaatgg tacatcataa
      961 tcagtgattt caaaaggacc atggaaaaat tccccagaat gaatagcatt agaattaatc
     1021 cattgcattt ccatgaacca gcaagctgct gtggagtaaa tttctccgta attaataccg
     1081 ctaccaattg tgtaaataat tttatctcgt ttttggtttt taccccattc tttagcagtt
     1141 atttcaaatt tgctcttttc agcaataaca agatcattta acttatcaaa ttcattcaag
     1201 caagtttccc attttttaga gccatctaaa acaagtaaaa tattaaataa taaaccataa
     1261 agaattcctt tatttaaatc actagcatca acatctttac cccaatcata gtgaacagga
     1321 tattcagctg cttcccatag aggagatcct tcttcattag ataaacaaat agtcattgct
     1381 ccaaattctc ttgctttttt tgttgcttct acagtttctg gtgttgtgcc agaatgggaa
     1441 caagtgataa ctaaagaatt ttcagataat ccttttggta tagcatagtt aaattcgtta
     1501 gcagtataaa catgacaagg tacagttaat tctttatcaa atagatattg accagccatt
     1561 aaaacggctt gtgaaccccc acatgctgta aaatatacat gatcaattgt cttttccttt
     1621 aacgctgcca ttgctgtttc aatctgttct ttgtgtagtt gtctcatttc tttatccctc
     1681 actctaatag gtattataaa gttatataac tttacgatac ctattatcta tgaaattgct
     1741 ttattttgca atactatttt tcaaaaaaaa ttttttataa caattttat
//
LOCUS       DAYYNG010000266         1762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000266 DAYYNG010000000
VERSION     DAYYNG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.272"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACHBFA_002931"
     CDS             <1..367
                     /locus_tag="ACHBFA_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9396329.1"
                     /translation="IEKSWRENFNELDPFINYIFENKRIFDLVLFQSAGTKYQDIVEK
                     ITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTM
                     LLTRRLYQFSVPGWIELLS"
     gene            497..1165
                     /locus_tag="ACHBFA_002932"
     CDS             497..1165
                     /locus_tag="ACHBFA_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFM9396330.1"
                     /translation="MLKINPLTLITLNIFFPVIIFLGKGNTFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            1176..>1762
                     /locus_tag="ACHBFA_002933"
     CDS             1176..>1762
                     /locus_tag="ACHBFA_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9396331.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIV"
BASE COUNT          600 a          229 c          293 g          640 t
ORIGIN      
        1 tatagagaaa agttggagag agaacttcaa tgaattagat ccatttatta attatatttt
       61 tgagaataaa cgtatattcg atttggtttt attccaatca gctggaacca aatatcaaga
      121 tattgttgaa aaaattacat catttgtgac aaaaaaaaca gtctgttgtc tagataagtt
      181 aaagactatc ggaatgatca atcaaaattt agtctttaat gaagaagaaa tccacttcta
      241 catttactca ttctacgcta cattctatga tatattgaag catagctatt caaaagaaaa
      301 gactatgcta ttaaccagaa gattgtatca attttctgtt cctgggtgga ttgaattact
      361 gagttaatta aaaaaagctt gcattgagaa ttgttctcag ttatgctttt tgataagatc
      421 gcactgttat ctgaataacg gtgcattttt atcagcttaa gataacagtg cgatcttatt
      481 ttttggaggt gaaagagtgt taaaaatcaa tccgttaacc ttgattactt taaatatttt
      541 ttttccggtc attatttttt taggaaaggg aaatacattt caagtggggt gtttcattat
      601 tgctacagtt ttagtattga tgtttcgtaa aataaagaca tgtatttatt tcatcttgat
      661 ttattcagtt ctgtttttag taatgaaaat tattgatgct atcaagatag agttttttac
      721 tatatttttt gggacatcct tatatattgt atttcgaatg ttgccggtcg taatgatctc
      781 atggttgttg gttagtgtct atactagcaa tgaattatta tcctctttag aaaaaataca
      841 tttaccgaaa aaaataatgt taagtattac agtagtgatc agattctttc cgacttatcg
      901 ttctgaaata agaatgatta aagaatcttt aaaaatgaga aacattcgct tgtctttttt
      961 tcagcctctg caatatttgg aatttctgtt agttcccgtt cttatacgtg caactatgat
     1021 tgctgaagaa atgaccgcta atgcaataac aaaggggata gaatctcctg tgaaaagaag
     1081 ttcttactat aaaacaaaca tgagcttatt agactatatc atcctgtttt ccgttactat
     1141 ttttttctta agtattttga tttgaggtga aagaattgtt tgaatttaac aatgtttctt
     1201 tttcatataa ggatgttaag gaacaagcgc ttgttgacct taatttttca ataaaaaaag
     1261 gcagtcttac attgattact ggttcttctg ggagtgggaa atccacacta ttaaagttaa
     1321 tgaatggact tattccaaga ctttatgacg gaaaattggt tggggaaatt ttatttgaaa
     1381 ataactcgct acaatcctta acacaagaaa gtatatcaaa aaatattggc tacgtaagtc
     1441 aagatcctag aggacaattc tttacaacaa atacaacgag tgagttagta ttttcgatgg
     1501 aaaactttgg atttacaaaa gagcatatgg ataaaagaat caatgaatta gctgaattat
     1561 tagatctttc aaatatatta gataaaaaca ttttttcgct atcaagtggt gaacggcaga
     1621 aaatttctat tggatgtagt ttatcgctaa actctaaatt aattatattg gatgaaccgt
     1681 cttcaaattt ggacttcaaa gcgactaaga aacttgctga cttactttgt gatttaaaaa
     1741 ataaaggatt tactattgtt at
//
LOCUS       DAYYNG010000267         1748 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000267 DAYYNG010000000
VERSION     DAYYNG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.273"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..519
                     /locus_tag="ACHBFA_002934"
                     /pseudo
     CDS             55..519
                     /locus_tag="ACHBFA_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
     gene            complement(581..1624)
                     /locus_tag="ACHBFA_002935"
     CDS             complement(581..1624)
                     /locus_tag="ACHBFA_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM9396332.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          598 a          335 c          351 g          464 t
ORIGIN      
        1 ttttggatag agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa
       61 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
      121 tcttttcagc caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa
      181 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
      241 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
      301 gaaatccgaa cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg
      361 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca ttgagtgatc
      421 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
      481 cgccaattgt tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat
      541 tgttccatgc gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa
      601 taaatagtaa agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc
      661 gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag
      721 tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa
      781 acaagctgtt gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt
      841 ttcgtttttt ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga
      901 atttttcaaa cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca
      961 tggcgaattc gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag
     1021 attggagtgt accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta
     1081 atccaagaaa agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga
     1141 tctgtccttt ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag
     1201 aaccacaaac agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt
     1261 ttttaccaat caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa
     1321 ttccttttac accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa
     1381 cagagaattc taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt
     1441 taaaaaaagt atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact
     1501 gtggcagata aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac
     1561 cgggtaagat ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt
     1621 tcatttgatc ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact
     1681 aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt
     1741 ttcgaata
//
LOCUS       DAYYNG010000268         1733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000268 DAYYNG010000000
VERSION     DAYYNG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.274"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1460
                     /locus_tag="ACHBFA_002936"
     CDS             291..1460
                     /locus_tag="ACHBFA_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9396333.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          635 a          241 c          274 g          583 t
ORIGIN      
        1 aataatatac taccatttgt tggtagtata atttaaaggt aataagttat tcttttatta
       61 ctatgtttgg gttttatagc aaagaaagtt taataagagt ttggacctag caaacaatat
      121 taatgaggtc attaaatctt attatcttta gtgatataac gaaatatcta cacagacaaa
      181 atgatagcgt aaaatatttt gtgtaaatag atatttaggg tagaaaaaaa gaaagtccat
      241 tctgtaaaat taaagttacc acaaccgaaa ttttatagga ggactttctc atgcccaatt
      301 ttactacaga aattatggaa acactaatca ataaaggtga tttagatgaa ttatttcgat
      361 gtcatttaga attagctgtt aattcattat tgcaggctga attaacagct tttcttgatt
      421 atgaaaaata tgatcgtgct gggttcaatt caggtaattc ccgtaatggg aattattcac
      481 gttcattcag aacagagtat ggagaattaa atttggtaat tcctagagat agaaatggta
      541 agttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta gaaaccacta
      601 ttattcagct atttcaaaaa ggaattacta tgtctgaaat ctctgaattg attgaaaaaa
      661 tgtacgggca tcactatacg ccacaaacca tttcaaatat gactaaaata gtttctgaag
      721 atattattgc ctttaaagaa aggtccttag aatccagata ctctgtcatt tttatggatg
      781 ctactcacat tcctttaaaa agacaaactg tttcaaaaga agcagtttat attgtaattg
      841 gcattcgatt agatggaacc aaagaggttc ttgggtttag tcttgctcca actgaatctg
      901 cttatgtttg gaaagagatt cttcaagacc taaaagaccg tggtttaaaa gaggttttat
      961 tagtcgtaac tgatggttta agtggcatta acgatagtat tcatagtgtt tatccaaatg
     1021 ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag gttcgtgtta
     1081 gtgatcgaca agaaatctgt agcgatttca aattagttta tcaagcaagt tcaaaagaag
     1141 aagcaaataa tcaaataagg tttatgatag ataaatggaa aaagcagtat ccacgagtgg
     1201 ttaaattgct catgaatcct gctatattaa ccttttataa tttccctccg tcaatcagaa
     1261 gaactattta ttcaactaat ttgattgaag gttttaacaa acaattaaag aaatatacca
     1321 aaagaaaaga gcaatttcct aatgaagaat ctctggagag atttctagta tctcaattca
     1381 acaactataa tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa atacatgata
     1441 cattagaatc aatgttttaa cttgatcaac agaatgggct tttccattta cacaaaatta
     1501 ttgacgctcc cgacaaaatt atgatcttgc gtaaagttat aaaatattaa taaaaatatt
     1561 ttattaatga aaagcttggt agcacggtta ttcatatgct aatttatttc actttatagt
     1621 aaggggagtt cttagtatat tgttcaaact taaatttcat ataatttaat tatgtaaatt
     1681 agctattcta agaacttcta cggataactg cccgtctaaa atttaaataa gag
//
LOCUS       DAYYNG010000269         1717 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000269 DAYYNG010000000
VERSION     DAYYNG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.275"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1155)
                     /locus_tag="ACHBFA_002937"
     CDS             complement(<1..1155)
                     /locus_tag="ACHBFA_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9396334.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..>1717)
                     /locus_tag="ACHBFA_002938"
     CDS             complement(1158..>1717)
                     /locus_tag="ACHBFA_002938"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9396335.1"
                     /translation="QYGTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFG
                     YELDLNELSEGDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKK
                     TAIVAHYKTLNEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQ
                     INDQNRRPETFDFDSKIWVITQEEK"
BASE COUNT          583 a          307 c          253 g          574 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaa
//
LOCUS       DAYYNG010000270         1701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000270 DAYYNG010000000
VERSION     DAYYNG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.276"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            466..1065
                     /locus_tag="ACHBFA_002939"
     CDS             466..1065
                     /locus_tag="ACHBFA_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9396336.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1701
                     /locus_tag="ACHBFA_002940"
     CDS             1129..>1701
                     /locus_tag="ACHBFA_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFM9396337.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          612 a          257 c          321 g          511 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga t
//
LOCUS       DAYYNG010000271         1699 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000271 DAYYNG010000000
VERSION     DAYYNG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.277"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1509)
                     /locus_tag="ACHBFA_002941"
     CDS             complement(28..1509)
                     /locus_tag="ACHBFA_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396338.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          524 a          231 c          294 g          650 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
     1621 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
     1681 aagatattct tgtttttaa
//
LOCUS       DAYYNG010000272         1697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000272 DAYYNG010000000
VERSION     DAYYNG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.278"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..1509
                     /locus_tag="ACHBFA_002942"
     CDS             259..1509
                     /locus_tag="ACHBFA_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM9396339.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT          544 a          267 c          374 g          512 t
ORIGIN      
        1 ttaaaatgaa gatttatatt tatgaattaa atatgataat tttttgaagt atttagtaag
       61 gtcgtatctt gttattaaag aatactctac atgtcttgtg gagaatagat aaaattagat
      121 gtgtggttta taaatgaaag agattttatc acattctgga aaattatttt atcaacattc
      181 taagctgtgt tattttgaca tgatgaatag ccgtaggagt aacattctcc atacttaaga
      241 gaggaaagag gagatattgt gaagaaaaag tacaacatcg cagtagccgg caccggttat
      301 gttggtttga gtatcgccac actattggcg cagcatcacc aggtaaccgc ggtagacatt
      361 atccctgaaa agattgaact gattaataaa cggaagtctc caattcagga tgaatatatt
      421 gaaaagtatc ttacagaaaa agaacttgac ttaactgcta ctttggatgc aaggagcgct
      481 tataaatccg cagatttcgt tgttattgct acaccgacta actatgacag tcgtactcag
      541 catttcgata cttctgctgt tgagtcagtt attgaggttg tgatggacgt gaatcctgat
      601 accatcatgg tcatcaagtc tacaattcct gttggctaca ctgaatcagt ccgtgagaca
      661 acggggagta agaacattat ttttagtcca gagtttctgc gcgagagcaa ggctttatat
      721 gataatctgt atccatctcg tattattgtt ggtactgata tgactgatga gcgcttgtta
      781 aatgctgctc atgtttttgc aacacttctg cagaaaagtg caattaagga gaacgtggat
      841 actcttttta tgggattcac tgaagccgaa gcagtaaagc tgtttgcaaa tacctattta
      901 gcactgcgag tcagctattt caatgaatta gacacttatg ctgaaatgaa ggggctgaat
      961 actcagcaga taatcaatgg tgtttgtctc gatcctcgta ttggaactca ctacaacaat
     1021 ccatcctttg gatatggagg ttattgcttg ccaaaagata ccaagcagtt gttggcaaac
     1081 tatgcagatg ttcctggaaa tttgattgag gctatcgttg aatctaatcg tacccgtaag
     1141 gatttcatcg cagatcgtgt tcttcagatg gcgggctatt acaactacgc tgaatataat
     1201 cagtatgatg tcaacaggga aaatccagtt ttaatcggta tataccggct gaccatgaag
     1261 agcaacagtg ataattttcg tcagagttca atccagggag ttatgaagcg cgtaaaagct
     1321 aaaggtgcga ctgtggtgat ttatgagcct gcactgaaag atggagagac ttttttcgga
     1381 agtaaggtta tcaacgattt agagcagttt aaggcgatgt gtgattctat tattgccaat
     1441 cgatatgata cctgcttgga tgatattaaa gaaaaagttt acactagaga tatttttcag
     1501 cgtgactgat agagaggtta atatgaacaa aaagatatgt tttgcggctt cctctggggg
     1561 gcattatgaa cagttaatga tgttaaaacc actgatggag aagtatgaga gttttgttat
     1621 aacagaacag acagaatatt cagctgagtc aaaaggcgaa aaaacgtact acatgagaca
     1681 agtaaataga aaagaaa
//
LOCUS       DAYYNG010000273         1653 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000273 DAYYNG010000000
VERSION     DAYYNG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.279"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..187)
                     /locus_tag="ACHBFA_002943"
     CDS             complement(<1..187)
                     /locus_tag="ACHBFA_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFM9396340.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYR"
     gene            352..1512
                     /locus_tag="ACHBFA_002944"
     CDS             352..1512
                     /locus_tag="ACHBFA_002944"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9396341.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT          566 a          302 c          248 g          537 t
ORIGIN      
        1 aacgatacac ggtaaatcca agaaatccgc caataattaa taatgtaaga gcaatcacta
       61 aaatgatctt cctcacaatc ttatcttctt ttttacgcag atttctccgc ttgtcgatta
      121 tatctgctgt agctgcctta ctggcttctt ttttatcttt acttgggtgt tgtttgtttc
      181 ctgacaaaac aactcctcct tactatcatc ttcgtaactc tgtttattat acatgaaatt
      241 tctactgccg tgaattattt taccaaatat tatgtcaaaa ttgtaagtgg caaatactct
      301 ttttgaggat tacaatgaaa aatagttaaa aatggataga aaggaattaa catgtcaaaa
      361 ggaaaacatc gaaaaaaaca aaaaatttct atttcttttc ttgtaatttt attattgtct
      421 gtcagcttct tttcaacagg catcattata ggaggtcaat taacaaaaat acctcattcc
      481 caaacttata aagcggagcc aacacaactt acttccaatt taacgcccga tgagcagagg
      541 cttgctagca aaattgatca attactacaa aaatcagatt atatcggttc gatttatgtt
      601 cgcaaaaata atcgcgtact attagaaaaa ggatatggat atgcaaatct gcgcgtaaaa
      661 gaatcgaatt caccttctat ttactatcaa atcggttcga ttcaaaaagc aatgactgct
      721 ttactgatac taaaacaaat ccaagctggt aagttaactt ttgacacaaa gttagcagaa
      781 ttttatcctc agattcctgg aagtcagcaa atcacaatca aaaatatgct ttatatgcgc
      841 tctggtcttc atcgaactgc ttctcccgac aaacctatgt cggatagtga aatcattcag
      901 ttcgcccttc atcatttgaa acttactgat tatgatacgt atcattatga accattgaat
      961 tatacattgc ttacagggat attgatcaaa ctaacgaatc agccttatga aactcttcta
     1021 aaaaaagaaa tcattcgtcc actccatcta gaacatacag atttttatga caatgtaaaa
     1081 aattcgcctc aacatgctgt atcttaccag atgtctgcaa ctgacgatta ttcaaaagca
     1141 ttgatcgagc ctgaaaccga catccgaaat gaattaggta caggaaacat aagtatgtct
     1201 gtttatgact tgaacaaatt ctttaccagt gttctttctg gcggtatcat accaaaagaa
     1261 ctacttttct cattgtggca aaataatggt gataaacatc cttacagcgg cggtgtttac
     1321 agtggaaacg attatatcct tgctcagggg aatatcaatc gattccatgc agtagtggcg
     1381 atgaaaaaag acactaaaga tgcaatcgtc atggaaagta atatccaatc ggataagaac
     1441 atcaaattgc cggccactga tttaagaaat cagatttatg aactgatgga aggtgttcac
     1501 ttatcaaatt aaattttgac ataatattag ttgtcatctc tgtatagtca atttagttag
     1561 tactctatct gaaaagtaga aataaattaa caaatattat ctgtccttat tatttgttaa
     1621 tttatttctt attttactta aataattttt ggt
//
LOCUS       DAYYNG010000274         1613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000274 DAYYNG010000000
VERSION     DAYYNG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.280"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            344..1543
                     /locus_tag="ACHBFA_002945"
     CDS             344..1543
                     /locus_tag="ACHBFA_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9396342.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT          448 a          273 c          327 g          565 t
ORIGIN      
        1 gaaatatatt ggtacaagat atagcaggaa tcgattactc gatccctgct ttttttaact
       61 tcctttgcag caaaaaaagc tcgtaatcta agagatgatg gatagttttt agaatatata
      121 tattttcttc aattccagaa aaaaatgacc aagctagtat gctaaaaaag tacgatgctt
      181 ctatgatctt ttttccgtct ttattaacag atgttttaat tgatcaacgc atattgtaat
      241 ctaccctaat ttccatagtt cttcaccact tttaaaaata gtaaaacccc tacaagtatg
      301 ctatagtaaa ttgctaaaga aaaaccaata aggtgggaga tgtgtgcgcg atcaaacaag
      361 cggaaaaatg attcgttttt attatacaag tttgcatttt ctttattgga tgatgttttg
      421 ttctgtctat gggtttgcga cattgttttt gatttatttc aagattgatc cagctaaaat
      481 cggagggatc ttagcattgg tcaatattgt ttcaacggtg cttcagcctt ttgtcagtca
      541 agtgatcatc aagaaaagaa agatcccact gatcaccgtg ctgaagacga tgatagcagg
      601 attaggagtg attctcttag ctggggtatt gattccgaat ttgccgatca ttacttatat
      661 cttgggtggg attttagtag tttctatgca atcatttctc aatgcgctgg catttgagta
      721 ttcaaatacc ggctacagga tcaactttgg cttttcaaga gctggtggtt cattttctta
      781 tgctgtaact tcttttttac tgggacagct gttagcgatt tattctgccg cgattttgcc
      841 aatcttggtt atagttgaag cagtgatatt tttcgttatt ttgcatctgc tgccggatgt
      901 aaaaaatcaa gaagtttttc ctatgacaaa agaacaggat cgctcagttt ctttgagaaa
      961 aaaatatcct ttcctgattt tgttattttt aggtttcagt tttcttttta ctttccacac
     1021 catgatcaac agctttttgg cacagatctt tgaaaatatc cacggcggca gtaaagaagt
     1081 tggcatcgca ttgatgatcg ccgctttatg cgaattaccg ggaatgatct tttttgaaca
     1141 gcttgtcaaa aagaaatcca gcaaattttg gctgtctgtt gccagtattt catttgctgt
     1201 cagaggattc atgatgctgc tggccagttc gatcctgatg atggaaatga cgcatctcct
     1261 acaagccttc agtttcgctt tgtttattcc agcttcggct tatttgttca atcaatttct
     1321 ggcggatgat gatcgtgtat ttggacaaac gatgatcatc atcgccacaa ctcttggtgg
     1381 ggtgatagga aatgtcttgg gcggcagtct gctgcagcat ttcggtgtat gggagatgct
     1441 gattttcggt actggttttg cggtgctagg tgctttatta actattttgg gcattcgaaa
     1501 aataccgaag acaggaaacg atagtgattc tcttgttgag tagcataaaa atacttagat
     1561 actagtttta tataaataac atatagtgat tattggcgta attacaaaaa acg
//
LOCUS       DAYYNG010000275         1591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000275 DAYYNG010000000
VERSION     DAYYNG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.281"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..953)
                     /locus_tag="ACHBFA_002946"
     CDS             complement(69..953)
                     /locus_tag="ACHBFA_002946"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9396343.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1048..1485)
                     /locus_tag="ACHBFA_002947"
     CDS             complement(1048..1485)
                     /locus_tag="ACHBFA_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396344.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT          502 a          331 c          243 g          515 t
ORIGIN      
        1 tcttgtcaca gtcccttatc ccgaacaagc ggtgttctcc cgccaactcc tcggtcacaa
       61 cctccatttt aacaataaac ctgtaaatgt gagggcaata ctttgattgt cacaggtaat
      121 ttatcccctt catctccatc aacattcgtc gtcaattctt cctcatcagc taatgacaca
      181 actaattgct cacaagtaag gtaggctaga tttttcgtag attcatctac tccttttaat
      241 aggtctggca atgttttaag cgtatcgatc aaagaagaat cttttaaata aaccaaatgg
      301 agcttaccat catccacttt tgcatttgga agtaatgttt caaaaccgcc gactgagtta
      361 gttagtccta tcagcaaggt actgctctca atttcttcct tctttccatc aacttctacg
      421 tgaaacgagt aggattgtgt ggaagctaac tgtttgattc ctgacatgaa ataagctagt
      481 tttccaaatt tcgtcttctt ctccgcatct acatcattga ttgattcagg gatagagcca
      541 attgcgacga cattcataaa atagtcgtca tttatttttc caatatccaa tggcttgact
      601 gattcaatcg aaaaatgatt gatcgcttcc tcaggttcta gcggtatccc taatgctcga
      661 gccaagtcat tcaccgtacc caatgggaaa aatccgaaat ttggccgatg ttcttgttca
      721 gcgattccac tgattccttc gttgactgtt ccatctccgc ccatgacaaa cacactatga
      781 tacccttccg tagccgcttc gcgagtaaag ttttttgcat cccctgcttt ttttgtatgc
      841 aagacgacca cttcatcaaa caccgattcc aattttgcta tcgctaattg ctcaaattcc
      901 tttgcttgtt caccgcctga actggggttt accactaata aaactttttt catcactttc
      961 tttcccttct gattgatttc tttaatataa cgatagcata aagaaaaaaa ttaggctgtg
     1021 atgaagtact gttcatgaaa cattcattta ttttttgaca agataaaaca tccatactat
     1081 gaaactgagg aaaattaaaa caatgatata ggaaataaaa gaaaatgata ggcattgtat
     1141 acctaataaa gtaaaaatct taggtggcag ataaaaacac aaaaatagca agagaatgta
     1201 atagcgtttc ttccactttt tgttatcaga ccttatagcg atcagaggaa taccaaatgc
     1261 ataactgaaa aactcttgat gtactgttaa tatgtcacgg ccttgaataa taaatgcgac
     1321 aatccccgct ataattagtg tgcctaacac caaaacggaa aattgatgaa tatattttat
     1381 atacaaatga tccattgtac ttcctgcctt tttatttgtg agaaaaacaa atccgtatac
     1441 gtgaataact tctagaaata aaagaaattt gcaaaaccac tccatttatc tacttctacg
     1501 tgcatttttt gatattgttt tctttattta agattataca gaaaaatatc tagggtcgct
     1561 ggattctaag taaaaaaaat ttttaaaata a
//
LOCUS       DAYYNG010000276         1583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000276 DAYYNG010000000
VERSION     DAYYNG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.282"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(217..519)
                     /locus_tag="ACHBFA_002948"
     CDS             complement(217..519)
                     /locus_tag="ACHBFA_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9396345.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDVVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(613..1407)
                     /locus_tag="ACHBFA_002949"
     CDS             complement(613..1407)
                     /locus_tag="ACHBFA_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HFM9396346.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGILLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT          528 a          251 c          200 g          604 t
ORIGIN      
        1 attaaaaagc ataccatatt tttgaaaaaa aagtgagaaa aaaggcttac aattggagtg
       61 gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa tatagatatc
      121 atcatattac acaatcttaa tactaactta aaaatatctc cttttataag tatggtgata
      181 aaatccgttc cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc
      241 aacgaaagta tactttatat aatcataacg ccgatgatcg cttcgtgcgt ctggcacgtc
      301 agtcacgaca tcaaacaaaa aatatacgtc tttcttcatt ctcgttttcg cagcagggat
      361 tttaaagtag ttcttattag aatagtagag attgattaat aagctatctt cgattgctaa
      421 aaagaaaact tctgaatccc acaccttata aaaatctttg acaaatctat tcgaaggatc
      481 aaatttaaac cataattgtg tttttccttc catcaacata atgttcacct caaaaagagt
      541 atacgaacaa atgtttgtat tgtaaataga aaaagatgag ctaaattaaa tgctcatctt
      601 ctttgataga tattataaat ctaataattc ttgtttcttt ttattaaatt cttcttctgt
      661 aatgattcca tcatccaata gttctttata ttttcttatt tctgaagctg aggaaattgt
      721 tgatgtattg gaatttgtag cagaaatatt tcttcttttt aaattataaa taatttcgtt
      781 taaagaatca cttatcctat caacagtatc tttatcaact gcaacattca atactttacc
      841 aagagtatca atagttaatg tacccaacag tattcctctt tttttactaa tattatttaa
      901 attgtctaat ataatacttt gtacattatt gcctatcatt ttttgttgag ctacaattaa
      961 acgtttatta gttaatgcgt atgcataatt attgtcatgc ttagttgacg agatatagtt
     1021 atgcaatcct acaaagcaga agattacgta ttcgtcactg ttaagttgat ctgatattaa
     1081 gttaaaatga tgagttgtcc attttttagt cattccttta ccatagccgt atttaagaca
     1141 aaattctacc atctcttcag tagttctcat tctatcatta cctatatcat gattgttttg
     1201 tgtagtataa tcagatatgt ttaattttct tttttctgat ggacccttat ctaaatcttc
     1261 taaattcaaa gatcttatta ttttataagg agtagtcata ccgtaaccag cttttttcat
     1321 acaattgccg caaacaattt gtttatcttt gattttatat gaagttaagc caattttacc
     1381 tccgcacttt gcacattgtt tactcatatt agttcctcct agatatcagc tttctactac
     1441 tattaatatt ctatcaatag tgaccatact ttgaccatac tttttagaaa gaatagaaaa
     1501 ttacaagata tttaataata gaagaaaaac actaatatca tgggttcaga agcattgaga
     1561 gcttatcagg aaacagtata att
//
LOCUS       DAYYNG010000277         1581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000277 DAYYNG010000000
VERSION     DAYYNG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.283"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(223..1242)
                     /locus_tag="ACHBFA_002950"
     CDS             complement(223..1242)
                     /locus_tag="ACHBFA_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9396347.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1253..1459)
                     /locus_tag="ACHBFA_002951"
     CDS             complement(1253..1459)
                     /locus_tag="ACHBFA_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9396348.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          440 a          331 c          274 g          536 t
ORIGIN      
        1 agccgtctgc tctgaccaac tgagctaagg gtactggttg ttgccacata aagccataaa
       61 caatcaacca gtagaatgtg tggcaacaaa cctgttatcg catagcttgg agtgtgacta
      121 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaatga tttttatagt
      181 tgtcaatata gttatgtaat gctcctcaac gaggagctat ttttatcgtt taggaatatt
      241 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
      301 attcgttgcc cgcatcatga catagacttt cttattaggg aagttacgca tgttaaaaga
      361 tacatgatag ccaacatttc cagaagtatt ataagcttga tttacatctg atctataaat
      421 tccatcagct cttactcgag ctaattcttt cccagtattg taatccataa tgaagatata
      481 ctcgtattta tagttagcaa tgtgccatcc agctacatgc aaatttgcat tttcgatttc
      541 tccaaactga tcaatgtggg cgtaattcgt tccatctgtc agtgtaggat tagctgcacc
      601 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggtaaa
      661 tccatgagct aaatcatatg ctaatttttc tttacttacg cccatttcag aaagataacc
      721 gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc cataaatgcg atttgattcc
      781 cggttggtta taaggagtgt ccaacgttaa tggaatacca tatttcattg ctgaatctct
      841 agccaattca acgtatgcct tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg
      901 taactcaatc tgcacaggac tgttggcatt agcatacgaa ccagcaccgt actgtacata
      961 accaggttga ccgacttgat aaacaattcc gccgtctccc acaatataag cagtataagc
     1021 gctagtccat gaacgttgca tatactgcgc ttcattgcgt cctgttgctg tttcattagc
     1081 cgtttcatgc agtaaaatat actgattatt tgctacttgt gagctacctt catttgcgcc
     1141 caaattaaat tcattgttga tagtataggc aaatccgtta attggcaata aaaaaagagc
     1201 cgttaacagg ctcatcgcag taatagtaat tttctttttc atatctatcc tcctatttat
     1261 ctgatgaaat tttcttatac gttcgatttg atacacctaa aatactccct agaaatgctc
     1321 caaaacctgt aataatcatc accgttatat ctgtgtaccg ccagttaaca ccttttccga
     1381 caagaccgac aaaagtcgct aaagcgggga taactactat agcgatccat ttcaaaatat
     1441 caaaggttct attgttcata ctatccacct cccttcattg gttgggctac tttctttttc
     1501 aatcgcttgt tttcttctat caatatttct ctttctgaaa gcaaggcatc aatttgcccg
     1561 tccagttgtt caattttatc t
//
LOCUS       DAYYNG010000278         1580 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000278 DAYYNG010000000
VERSION     DAYYNG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.284"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..1530
                     /locus_tag="ACHBFA_002952"
     CDS             64..1530
                     /locus_tag="ACHBFA_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM9396349.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          437 a          291 c          332 g          520 t
ORIGIN      
        1 gttaaatttt tcggtaaaga tgtataatat tttctgaaaa tttaaagaat tggggtatag
       61 attatggaaa aacagcaaga caagggcttc ttcgggcagc cgaagggatt gtcaactttg
      121 ttcttcactg agatgtggga acgatttagt tattacggta tgcgtgcatt acttatctat
      181 tatatgtacg atacagttgc caacggggga cttggattac cacgagcaac agcattagct
      241 atcatgtcga tctacggatc tttgatctac atgtctagta tcgtcggcgg atgggtatct
      301 gaccgagtgt taggagcaaa acgcactgta ttcttcggtg gagttttcat tatgcttggc
      361 cacatcgtct tggctttacc atttggtgta agtacattgt ttgtttcgat ggcattgatt
      421 attatcggaa ccgccttttt gaaaccaaat gtttctggca tggtcggaca tctttattca
      481 aagactgact tgcgaagaga tgcaggattt tcgatttttt acatgggtgt caacttaggg
      541 gcattattag caccactaat cgttggtaca atcggtcaag aatataacta tcatttaggg
      601 ttctctttag ctgctattgg gatgttcttt ggattattac aatacatcat ccaaggacgc
      661 aagacattag acggagtggg tacaacaccg ccgaatcctt tgacaactga agaacgtaaa
      721 ggattcatcc gtaacttgat catcgcactt gttgttatcg ctgcagtatt cggcggggca
      781 caactaactg gtcatctgac aattgacttt tttgtcaata caatcagtgt attggggatt
      841 atcttaccgt tgtattactt tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa
      901 aaaccaagag tatgggctta tatcccatta ttccttgcag caatcatttt ctggtcatta
      961 caagaacaag gttcatctat cttagcttta tttgctagtg aacgtacaca atctacgcta
     1021 tttggtgtaa cgatccaagc aagctggtat caatcattga atccaatttt catctttatt
     1081 ctgacaccag tattcgtaac gatgtggacg aaacttggtg aacgtcaacc atctacagta
     1141 gtgaaattct ctcttggact tttatttgca ggattatcgt acttgctatt gatgttgccg
     1201 ggtatgcttt atggtacaag tggaaaagta agtccattgt ggttgttggg aagtttcttc
     1261 atcgtggtta ttgctgaatt gtgtctttca ccagttggtt tgtctgtgac aacaaaatta
     1321 gcaccacgag catttgaatc acaaacaatc gctatttggt tcttagctga tgcttcttca
     1381 caagcaatca atgcacaaat tgcacgcttc tacacaccgg gaacagaatc tgcttacttc
     1441 ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc tatttactgt gaaagaacca
     1501 atcaaaaaac taatgggctc tattcattaa tcaatgaaca taaaaacagg aattgcttat
     1561 ttgcagcagt tcctgttttt
//
LOCUS       DAYYNG010000279         1561 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000279 DAYYNG010000000
VERSION     DAYYNG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.285"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHBFA_002953"
     CDS             131..505
                     /locus_tag="ACHBFA_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9396350.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHBFA_002954"
     CDS             complement(619..1248)
                     /locus_tag="ACHBFA_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9396351.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHBFA_002955"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHBFA_002955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          535 a          232 c          271 g          523 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 t
//
LOCUS       DAYYNG010000280         1469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000280 DAYYNG010000000
VERSION     DAYYNG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.286"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..>1469
                     /locus_tag="ACHBFA_002956"
     CDS             114..>1469
                     /locus_tag="ACHBFA_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396352.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CL"
BASE COUNT          526 a          203 c          270 g          470 t
ORIGIN      
        1 aataactgat gaaaaaacaa gcataatcca tcataaaatc agtataggtg aaaagatgaa
       61 ctttcatctg catatcaagt aattggtatg aggcattttt tgaggtgtag gatatgaagt
      121 tggattacct tatagaagag gaagatagag aaaaactgct gctagctaga tatttagaat
      181 ttgagaaaaa gaattatgta gtgctgccta agttatatga aacattaggt ttgtctaaat
      241 ttaaaacaag aaattatatc atagaattaa ataaagaatt tactgaattc aaaacaaagc
      301 cagagatcca aatttcagaa gaagcagaga tccatgtcga acgtctgaat ttgacggata
      361 tcaaaaattt aagaagaatc tactttcaac gatcaaaaat tgctcgactg ttaacggaga
      421 tcgtggattc aaaagtaacg atcgaaaaat ttgcagagaa tcatttttta agcatatcaa
      481 gagcctatgt aaaaagaaga gaattagtct cttttcttga acagcatcaa ataaagttaa
      541 agaaaaacat attagttggg gaagaaataa acatacggaa cgtattattt ggggtatatt
      601 tcggggtctt taatggtttt tctatgccat ttagagaaag cgtcatgaat gaagcaaaga
      661 gtatcgtgca atattttgaa tatctgttca atttaaaact gtctgcaaca aaacgaatca
      721 aattcaattt gctaaccact attagtttgt tgagggccta tgggaatcaa ccaatcggaa
      781 aacgtttctt ttcagatgaa tactttaaga gctcagaagg aatgaagatc atcaaggatt
      841 tatcacatat ttcttttctt catatcccta aagagcagat cattgatgag atttcttata
      901 tcatgttgtt tttgatcacg gaagaagaat ttgaattaga tggcgcattt ggacttgcgg
      961 aagctcattt tcatgatatc cacatgatta gtcaaaaaat gtcagtggaa ttgattgatc
     1021 aattaccatt ttcgattgaa atctctgaag aagaaagact atatatacga aaagaacttc
     1081 gtatgggtat gatgcatgtc aatcgaaaaa attggatatt cgactttgag atgactagct
     1141 ttacgtctaa aaagcagcta cagtttttct tcgagacgta tccaatattc agtacagcaa
     1201 tccaaaaggt catacaagag aataaacgct atttcttggg aaaagatgaa cgtttcatga
     1261 ttcgcttatt ttatgattat ctctttttat tggtcggtat ttgtccggta tcatattttg
     1321 aagcaccgat tcatgtctgt attgattttt cgcaaggatc tgcttatacc gattacatta
     1381 tttctcaagt gaaaggattt aaaaattaca atattgtgat tgagagtcga atcacttctc
     1441 gtacgaatat ttatttatct gattgttta
//
LOCUS       DAYYNG010000281         1467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000281 DAYYNG010000000
VERSION     DAYYNG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.287"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACHBFA_002957"
     CDS             <1..189
                     /locus_tag="ACHBFA_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9396353.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="ACHBFA_002958"
     CDS             complement(275..1369)
                     /locus_tag="ACHBFA_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM9396354.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          447 a          303 c          227 g          490 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaata
//
LOCUS       DAYYNG010000282         1445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000282 DAYYNG010000000
VERSION     DAYYNG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.288"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..378)
                     /locus_tag="ACHBFA_002959"
     CDS             complement(193..378)
                     /locus_tag="ACHBFA_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396355.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1228..>1445)
                     /locus_tag="ACHBFA_002960"
     CDS             complement(1228..>1445)
                     /locus_tag="ACHBFA_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9396356.1"
                     /translation="RKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDF
                     KERKLKKIENLPLQDMAKIDQIIQYMF"
BASE COUNT          492 a          221 c          209 g          523 t
ORIGIN      
        1 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
       61 ctatcatttt tatcctcaac tacatacata tctgttgcaa cttcactttc tgtctcatcc
      121 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
      181 ccaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
      241 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
      301 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
      361 tttagaattt ttactcattc ataaacactt ccttaaatag cttttattat agaaggtatt
      421 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaccttaca ttatactgga
      481 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
      541 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
      601 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
      661 gcacattttc gagcattaca agtattggtg tcgttagaac aactaaatag aaactagcgg
      721 tagatttttc agaatctatg ctaatttctg tacggatgtt ccagtgatgg caaatttctc
      781 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
      841 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
      901 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
      961 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
     1021 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
     1081 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatccc atcgaccaca
     1141 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
     1201 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
     1261 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
     1321 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
     1381 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
     1441 acggt
//
LOCUS       DAYYNG010000283         1424 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000283 DAYYNG010000000
VERSION     DAYYNG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.289"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1424
                     /locus_tag="ACHBFA_002961"
                     /pseudo
     CDS             4..1424
                     /locus_tag="ACHBFA_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          590 a          223 c          318 g          293 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaa
//
LOCUS       DAYYNG010000284         1422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000284 DAYYNG010000000
VERSION     DAYYNG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.290"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..446
                     /locus_tag="ACHBFA_002962"
     CDS             36..446
                     /locus_tag="ACHBFA_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9396357.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            460..816
                     /locus_tag="ACHBFA_002963"
     CDS             460..816
                     /locus_tag="ACHBFA_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9396358.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          525 a          209 c          265 g          423 t
ORIGIN      
        1 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
       61 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      121 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttagcagcga
      181 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      241 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      301 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      361 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      421 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      481 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      541 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      601 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      661 attgcgattg acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat
      721 accaatctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      781 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      841 ctgagcattt catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      901 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
      961 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1021 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1081 ttgttgacat gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg
     1141 tgaagcttga ttctatcgaa aaaaacttca aaaaaagttc ttgacaaata acaaatgata
     1201 agttatgata aatgagttgc tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa
     1261 aagagttgac aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa
     1321 cgaatcgggg aacactcctg atgaagtaga cctttgaaaa ctgaacaaag taagacaaac
     1381 caaatttgta gggcgtcttg attcaattca agacaacaaa ca
//
LOCUS       DAYYNG010000285         1368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000285 DAYYNG010000000
VERSION     DAYYNG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.291"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1368
                     /locus_tag="ACHBFA_002964"
     CDS             82..>1368
                     /locus_tag="ACHBFA_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM9396359.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAYYNG010000286         1359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000286 DAYYNG010000000
VERSION     DAYYNG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.292"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..484
                     /locus_tag="ACHBFA_002965"
     CDS             <1..484
                     /locus_tag="ACHBFA_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9396360.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1215)
                     /locus_tag="ACHBFA_002966"
                     /pseudo
     CDS             complement(691..1215)
                     /locus_tag="ACHBFA_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          445 a          254 c          260 g          400 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc agtagaatag agttaccaca acacattcac agaaaagagg acttccatat
     1321 gaacgatttt actacagaaa ttctaaagac tctagcgaa
//
LOCUS       DAYYNG010000287         1354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000287 DAYYNG010000000
VERSION     DAYYNG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.294"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..688
                     /locus_tag="ACHBFA_002967"
     CDS             50..688
                     /locus_tag="ACHBFA_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM9396361.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(807..879)
                     /locus_tag="ACHBFA_002968"
     tRNA            complement(807..879)
                     /locus_tag="ACHBFA_002968"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(844..846),aa:Thr,seq:cgt)
     gene            1000..1242
                     /locus_tag="ACHBFA_002969"
     CDS             1000..1242
                     /locus_tag="ACHBFA_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396362.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
BASE COUNT          443 a          218 c          239 g          454 t
ORIGIN      
        1 catgtatact gactctatct tcaaaaactg aatagtagga gttattatca tgaataacat
       61 tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc actattgtcg ttgtactcgg
      121 tttgttaggg ttgctatttg cgccatccat tattagcatt ttttctttaa ctatcactgg
      181 atttatagca tttacacttg tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc
      241 tgtcgctcct gtcaaaaata tagccaaata tttttttatc aatgtagtaa tcagtgccgc
      301 agtctcggta tttttgcaac aaattttgaa atggggttta acaggcaatc caataaatga
      361 ggctttcagc ccgttattat ttattatttt gcctatcatg atacttggag aagaattgtt
      421 ctcagtgtat tttctcgcta ttttttctag taagttcagc attcctgtcg ctagtttctt
      481 gtcagcaatc atctttggat ttatccatta cagtacttat gataatggaa atattttaca
      541 cactgtagca cacatccttt tgatacaagg ggtggcaaga ttgttattca accaagctgc
      601 cattaaaagc aactcgatca ttacaagttg ggcagttcat gtgatatttg atttcacagc
      661 tatattgcta gtcgttttat tctcctaaat ttttatagaa ataatacatg ctctattgct
      721 aagaaagcat actttatttc ctttaaacaa aaagacagat gtatcaatgg attaaaaatc
      781 cttgatatat ctgtcttctc ttttaatgcc agctataggg atcgaaccta cgacctacgc
      841 gttacgagtg cgttgctcta ccaactgagc taagctggcc ttatccaaaa gaaattatat
      901 actctatttg gctgattgta aagagtccga tggaaaatta gtttaatttc atgatatttt
      961 tcataaaacg tggtataatt gatgtgaaag gcaggtttta tgaaaatggc aacttttgga
     1021 aaatttgata caactatcgt accatcggaa attggtgaaa aattcaagat caatactcac
     1081 gtaatgttca caattcacgg caagtcgcaa actggaacga tttcaaagca attgaaaaat
     1141 tccgctgttg ttgagattga tgaaactgca gaaaatgcga acttgatgac gcaaagtaag
     1201 ggtgtagtaa tcgttaacta caaagaacta aaaaagattt gaatttgacc atctctgcat
     1261 ttttgggatg gtttttttat atgcaaacga aagctgtgaa aaagctgttc tgcatcaaac
     1321 gttaaaaatg accaaaaaag gatgtgacaa aagt
//
LOCUS       DAYYNG010000288         1335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000288 DAYYNG010000000
VERSION     DAYYNG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.295"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..245)
                     /locus_tag="ACHBFA_002970"
     CDS             complement(<1..245)
                     /locus_tag="ACHBFA_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9396363.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            483..>1335
                     /locus_tag="ACHBFA_002971"
     CDS             483..>1335
                     /locus_tag="ACHBFA_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9396364.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEE"
BASE COUNT          474 a          217 c          228 g          416 t
ORIGIN      
        1 tcttgccata cctcctgaat ttgaccatca ggagtcgtaa tgaaaatagt atcattttca
       61 tctactaatt ctcttaagtt taaatattgt ttactagatg taaaattcga agctgctaat
      121 gacgagtctt cgcttttact ataaaatcct gttacttgta ttttatgttc ctggaaatac
      181 ttcccgaatg aacaacctac cttgccggct ccaataaaac caacgttcat aattatcacc
      241 tccataaaga agtgagacta caagtcccat tcttacgata tgagcttaaa aataaaaatt
      301 aaatcaaata tacagtatgt ttttttcaaa tatcatccat agttaattta tcataatatc
      361 ccatatttta aaataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
      421 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      481 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      541 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      601 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      661 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      721 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      781 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      841 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      901 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      961 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
     1021 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
     1081 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
     1141 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
     1201 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
     1261 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
     1321 attcaaaaga agagg
//
LOCUS       DAYYNG010000289         1328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000289 DAYYNG010000000
VERSION     DAYYNG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.297"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..297
                     /locus_tag="ACHBFA_002972"
     CDS             82..297
                     /locus_tag="ACHBFA_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396365.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(597..839)
                     /locus_tag="ACHBFA_002973"
     CDS             complement(597..839)
                     /locus_tag="ACHBFA_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396366.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(826..1254)
                     /locus_tag="ACHBFA_002974"
     CDS             complement(826..1254)
                     /locus_tag="ACHBFA_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9396367.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT          444 a          262 c          208 g          414 t
ORIGIN      
        1 gaccaccacg gtacccctta aaattgtaaa aaaacactcc agtactccat caaaacggac
       61 tagaaaagag gaatttttaa tatgaaacaa ggaattgcag acataaaaat aattaaagaa
      121 attcttgaaa aatctacggc taatgcaata gcattcggaa caggaatcaa cttgagtaca
      181 gtaaaaaaac ttaaaagtgg agagagagct gaagaaaaat taaatcttgc cgatgctatt
      241 aaaatcacag aatttggcat gaaaaatatg ccaaccaaaa tcgaaatatg gaaatagtag
      301 tttttctgat ctatcagctg ttttgccaaa gcacatagga atctcacctc cccccagtaa
      361 tggcgggccg atttggttac ggaagtatca ttatcgtagt cgcgattctc atattaaaat
      421 ttgtcgctta aaagctaacc ttgtcatggc gtaactagag aatcaaaatc tacatgtact
      481 gatagatttg ctattcattt gtcaaagagc gctaggctat tttcagccta tggaaatcct
      541 ttattttgac ccgataaaaa acttccatag aatgaaaagg aaagggaaaa aatactttaa
      601 cgagttgtat caaattcaag tatcttctca atcaattttg tttccagttg tctacgcata
      661 tattcatcta catacataac cattcctttt tcttcttcat aaactggttt catggctaac
      721 cgtgaaatat aattttgata ttttgacaga acgatggaca tggcaattga atcaccagaa
      781 gctgcggctt tgattgtcgt tagtggtatg aatggtatat atcgtttatt ttgcttcata
      841 tttgacaccc aaatactttc ctagctcttc caaagccatt tgtcgttggt accaaacact
      901 gccgagactt ttcccaataa tatcgccaat ttcacgatca ttaagaccga tgaagaagga
      961 cataaaaatg atttcttgtc tttgactagg taatgctgca atcgcatttg ccaactcctc
     1021 attggaaata aagattttca taccaaggac aataaaagta ccactactat tcaaatttgg
     1081 ttccaaataa tagaactctg tacctaaccc tttttgttcc aatatttcca ttgatatttg
     1141 ccgttggcga aatctagcgt acttcttttg aatatttaac gcctcattgc agactacttt
     1201 cttacaaaaa ctgtcaaacg tatgttgaat gtgggtttcc tctgagtcgc ccataatatt
     1261 ctccccctca aaaaattcat tcaactcgtt ttacttctat ccctttccat tattactaca
     1321 atctagga
//
LOCUS       DAYYNG010000290         1326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000290 DAYYNG010000000
VERSION     DAYYNG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.298"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..368)
                     /locus_tag="ACHBFA_002975"
     CDS             complement(78..368)
                     /locus_tag="ACHBFA_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396368.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(486..746)
                     /locus_tag="ACHBFA_002976"
     CDS             complement(486..746)
                     /locus_tag="ACHBFA_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396369.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(883..1296)
                     /locus_tag="ACHBFA_002977"
     CDS             complement(883..1296)
                     /locus_tag="ACHBFA_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM9396370.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          486 a          227 c          197 g          416 t
ORIGIN      
        1 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
       61 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      121 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      181 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      241 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      301 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      361 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      421 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      481 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      541 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      601 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      661 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      721 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      781 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      841 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      901 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
      961 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1021 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1081 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1141 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1201 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1261 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1321 tttttt
//
LOCUS       DAYYNG010000291         1311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000291 DAYYNG010000000
VERSION     DAYYNG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.299"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHBFA_002978"
     CDS             200..742
                     /locus_tag="ACHBFA_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396371.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1311
                     /locus_tag="ACHBFA_002979"
     CDS             898..>1311
                     /locus_tag="ACHBFA_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM9396372.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMA"
BASE COUNT          532 a          148 c          255 g          376 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAYYNG010000292         1266 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000292 DAYYNG010000000
VERSION     DAYYNG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.300"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACHBFA_002980"
     CDS             85..624
                     /locus_tag="ACHBFA_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396373.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          476 a          163 c          201 g          426 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataat
//
LOCUS       DAYYNG010000293         1227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000293 DAYYNG010000000
VERSION     DAYYNG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.302"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..945
                     /locus_tag="ACHBFA_002981"
     CDS             100..945
                     /locus_tag="ACHBFA_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9396374.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT          474 a          146 c          210 g          397 t
ORIGIN      
        1 gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact
       61 tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt
      121 ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca
      181 agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta
      241 gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat
      301 ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat
      361 gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga
      421 agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt
      481 gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc
      541 ccttataata atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt
      601 atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta
      661 aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct
      721 aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc
      781 gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca
      841 atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat
      901 aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt
      961 atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact
     1021 tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg
     1081 tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt
     1141 tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata
     1201 aaagaaaaaa aggaggaaat ttttaat
//
LOCUS       DAYYNG010000294         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000294 DAYYNG010000000
VERSION     DAYYNG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.303"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHBFA_002982"
     CDS             414..947
                     /locus_tag="ACHBFA_002982"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9396375.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYNG010000295         1174 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000295 DAYYNG010000000
VERSION     DAYYNG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.305"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(485..859)
                     /locus_tag="ACHBFA_002983"
     CDS             complement(485..859)
                     /locus_tag="ACHBFA_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9396376.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(882..>1174)
                     /locus_tag="ACHBFA_002984"
     CDS             complement(882..>1174)
                     /locus_tag="ACHBFA_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFM9396377.1"
                     /translation="FRISLINNQLTFGKTPNYPWPQMFDKGLQRPLFLEIGKEYELKL
                     IVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDYSINYECGF"
BASE COUNT          391 a          220 c          152 g          411 t
ORIGIN      
        1 aacctccaga cttcttgata gctcaagtat atcttgaggt gcatttgtta gtaaggtact
       61 gttctatggg gcgcttactc taggagtgag catttttcga aaaatactaa aaatttttca
      121 aaataaaaaa aggcatacag acaataaact gtctatacgc ctcacagact ctattctata
      181 catctaattt tggctgtcct gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt
      241 tggaaaacat cattataact ttgcttggta agagctttgc taaaacatga tatgacttgt
      301 atacaaaatg tggagtgtat tccaataaat aggaaggatt acaaaatttg aaaccaaata
      361 agtaaaaaat actgtcactt tgaattaatt tgaaatagta aattctcatt aaactattga
      421 aatgctgata ccaaaaaatg aagtactttt cgttcatacc aacttagagc acttcgttat
      481 ctatttattt cttcatttgg cttatctcta taatctcatg atttggtccg tagaaattaa
      541 agtaacggat tccattttcc cagaacggta attccataat atttttatct aataattcat
      601 atcctaaatc ttgaattgaa ttgaatacct catctatgtc atccgtatct aatgctagat
      661 gatcaatggc tcctctacac tcagtagttt tatcttccca tatttctatt atcgttccat
      721 tcaactcgaa aaagccgact ttacttccca cattcatttc ttcattaatc agactaaacc
      781 ctaaggattc ataaaagatt tttgaagctc caaaatcttt tgttggaatt ccaatatgtt
      841 gtaaaccatt taatttcact tacatcatcc cttcaattgt tttaaaatcc acattcataa
      901 tttattgaat agtctagttt atctaccttg attgctccct ccgtaacagt catacttaat
      961 gctgttccga ctttatcata cattctagta cttaaagcaa cattatcaat atataaaaca
     1021 gctatcgtat catcaacaat taactttagt tcatattctt tgcctatttc caaaaatagt
     1081 ggtcgttgca gccccttatc aaacatttga ggccaaggat agttcggagt ttttccgaaa
     1141 gtaagctgat tattaatcaa tgaaatacga aatt
//
LOCUS       DAYYNG010000296         1160 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000296 DAYYNG010000000
VERSION     DAYYNG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.306"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..314
                     /locus_tag="ACHBFA_002985"
     CDS             54..314
                     /locus_tag="ACHBFA_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396378.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(676..915)
                     /locus_tag="ACHBFA_002986"
     CDS             complement(676..915)
                     /locus_tag="ACHBFA_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396379.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          414 a          164 c          211 g          371 t
ORIGIN      
        1 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
       61 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
      121 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
      181 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
      241 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
      301 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
      361 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
      421 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
      481 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
      541 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
      601 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
      661 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
      721 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
      781 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
      841 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
      901 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
      961 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
     1021 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
     1081 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
     1141 tatgatatta tctagaggta
//
LOCUS       DAYYNG010000297         1150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000297 DAYYNG010000000
VERSION     DAYYNG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.307"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..786
                     /locus_tag="ACHBFA_002987"
     CDS             28..786
                     /locus_tag="ACHBFA_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9396380.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT          396 a          200 c          194 g          360 t
ORIGIN      
        1 attatcctaa ctaatcggag gcaaaatatg gatacaaaat taagtgaatc ggagaaatat
       61 ctctgggatt ttattcaaag tcacatactc gaaatcccct cctattccat tgtaaaacta
      121 agtgaatatg ccaacgtttc aacagcaacc gtcgttcgta ctatgaagaa aaaaggatat
      181 gaggggttta cgtcttttaa acattcgtta aaagaaaaag aaaatacaaa tatcaatttt
      241 gccgctcttg ataaagttga tgaaggaata aagactgcta ttcttaaaaa tgagcaagag
      301 gtaatccgca ccatcaatat ggtagaaagc gggaatattg aagatgctat tcaacgaatc
      361 aaggctgcta gaagaatcat gatttttgcc cgaggttttt cagaattgat tgctcaggaa
      421 atgatggtca aatttcaact taccggtaaa tattgtgaga tgcatactga tcccaatatc
      481 ataaaaaaca ttagtagaaa attatccaaa gatgacatcg tgatctttgt ttcactaaac
      541 ggtgaaacaa aggaattgac taccgcagct gaaaactgtt ataaagctga gatcgggaca
      601 attttgctta ctgctaatcg tcattcaagt ttgatggaac atatcgagat tccttttgtt
      661 ggctttaaat cagaaggctc cttcttccca gattatgagg tacgctccag acttccactt
      721 tctatactgg ctagaatctt acttgactcc tatgcactgc gaacatcagg attcacacag
      781 cactaactct atatctgtaa gactgtacct aaaatatatc tgtgtgaatt aactaaggtt
      841 gtctagaaaa atatttaatc gctatttgta ttctcgtgct ttctatcatt gctccttcta
      901 taacttctta gtttcaagat agaatagtgc attgagacta ccaggactac ttacgcaaat
      961 atataataat aagaatgtga taagcagttc gaagttcctg tctaatattt cttgttaatt
     1021 attatcaatt tccaacaatt ctagaattat aatacgcaaa aaaaggagac cgtctaaccg
     1081 tctccttata gtttattatt cagattacaa tcagttatct aaaatgttac agaagtttta
     1141 ttagggatat
//
LOCUS       DAYYNG010000298         1137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000298 DAYYNG010000000
VERSION     DAYYNG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.308"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..965
                     /locus_tag="ACHBFA_002988"
     CDS             21..965
                     /locus_tag="ACHBFA_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9396381.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          396 a          162 c          223 g          356 t
ORIGIN      
        1 gtgcaattgg agtgaatttg atggacacta tattgattac tggcggagct ggttttatcg
       61 gttcaacgct agcgaattat ttgggaaaag aaaacaaaat cgtagtagtt gacgatttat
      121 caatgggaaa aaaagaaaac ttggatatgg ataaacaagt gactttcatt gagggggatg
      181 tatcagattc tcaattgatg gaaagaatca tgaaagaata tcagtttgcc tatatttttc
      241 atctagctgc tgtagctagt gtggcagatt ctgtagaacg gcctgtagaa acacatcgtg
      301 taaattttga cagtgcgctt ttattgcttg agttagcacg gaaatatcaa tctaatttaa
      361 aacgattagt atttagttca tcagctgcag tttatggtga tgaaccaaca ctgccaaaaa
      421 aagaagaatc agtcatccgt ccattaacac catatgctat cgataaattt gcagcagaac
      481 aatatgtttt ggattactgt catttatatg atgttccgac tagtgctgtc cgatttttta
      541 acgtttatgg tccaaatcaa aatccaaact ctccatatag cggagtcatt tcgattctag
      601 tggatcgtta caaaaaacaa ttggcaggag aaaaagcgga atttacttta tttggcgatg
      661 ggagtcagtc tagagacttt gtttatatag aagatgttat acaagcactt ttacttgttg
      721 ccaaggaaga aaaagcttta ggacagcaat tcaatgtcgg tacaggaaaa agcactacgc
      781 tacttgaatt gatccattca attgatcaaa tcttaggaac agagctagcg cttaaatatg
      841 aagcggagcg ttcaggggat attcgtgatt ccttagcgga tatttcaaaa attcgttctt
      901 taggctatca gcctaaattt gatatattga gcggaatgga acggtattta aagacagaaa
      961 tttgatgaaa aaatgattgc atctttggat aaataattct aaaaagtaaa acaagaatta
     1021 aagttgtata accaactatt taattcttgt tttacttttt atttaggaaa tatataatta
     1081 aatattttac atgaaaaaga gaactttata aaattcaatt atgacataaa agccaaa
//
LOCUS       DAYYNG010000299         1135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000299 DAYYNG010000000
VERSION     DAYYNG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.309"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..>1135
                     /locus_tag="ACHBFA_002989"
     CDS             66..>1135
                     /locus_tag="ACHBFA_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFM9396382.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESM"
BASE COUNT          347 a          234 c          256 g          298 t
ORIGIN      
        1 aaaaaggaag tcccttctgt agaatagagt taccacaaca cattcacaga agagaggact
       61 tccctatgaa cgattttact acagaaattc taaagactct agcgaacaaa ggcgatttga
      121 atgaattatt ccgtgtccat ttggagaaag cagtcaatac gcttctcaaa acggagttaa
      181 cggctttcct agattacgaa aagtatgatc gcattggttt taacacgggt aattctcgta
      241 acggctccta tgaccgtacg gtcaagaccg agtatgggga acttcatctc cagattccgc
      301 gcgaccgcaa tggtgagttc aagcaacaga ctgttcctgc ttatagacgg acgaatgaca
      361 cgttagagga gaccgtcatt cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag
      421 acttgattga gaaaatgtat gggcattact acacgcccca aaccatgtcc aatataacaa
      481 aatcatttac agaagaggta acggcgttta atgggcggga gcttcatgac cgttatgctg
      541 ctatttatat ggacgcaacg tatattccgt taaagcggaa aaccgtcgcc aaggaagcta
      601 ttcatatcgc agttggcatt cgcccggacg gatcaaagga agtattgagc tatgcgattg
      661 caccgactga atccatcacg atttgggagg aaattttatt ggaccttcaa gagcgcggtt
      721 tgaaaaatgt cctcctgttc atcacggatg gcttaaaggg gatggtagga gcgattagtc
      781 ggttctatcc caaagctcgt tttcaacatt gttgtgtaca cgtttcccgt aatatcagtc
      841 ataaagtgcg tgtcgatgat cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag
      901 cctcatctaa agaggtggcg ttggaagcac gtggtacttt tgcggaaaaa tggaaaacca
      961 gctatccaaa agtggttgaa tcgattcttt cgaacgatca cttgctcact ttctatgatt
     1021 tccccttggc catacgcaag agtatttatt ctacgaactt gattgaatcc tttaataaac
     1081 aaatcaagaa atacagccac cgcaaggaac agttccaaaa tgaagagtcg atgga
//
LOCUS       DAYYNG010000300         1132 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000300 DAYYNG010000000
VERSION     DAYYNG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.310"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..465)
                     /locus_tag="ACHBFA_002990"
     CDS             complement(<1..465)
                     /locus_tag="ACHBFA_002990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9396383.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDY"
     gene            complement(487..1089)
                     /locus_tag="ACHBFA_002991"
     CDS             complement(487..1089)
                     /locus_tag="ACHBFA_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9396384.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          362 a          258 c          161 g          351 t
ORIGIN      
        1 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
       61 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
      121 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
      181 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
      241 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
      301 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
      361 cacttgtttt tgaccaatga tcggcacaaa agcttccggc catacttctt gtatgactgc
      421 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
      481 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
      541 acccaccgac catggtttca agaaaatcac aatctgcata aattccctaa aagataattt
      601 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
      661 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
      721 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
      781 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
      841 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
      901 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
      961 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     1021 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     1081 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc ct
//
LOCUS       DAYYNG010000301         1131 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000301 DAYYNG010000000
VERSION     DAYYNG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.311"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..419
                     /locus_tag="ACHBFA_002992"
                     /pseudo
     CDS             1..419
                     /locus_tag="ACHBFA_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
     gene            403..795
                     /locus_tag="ACHBFA_002993"
     CDS             403..795
                     /locus_tag="ACHBFA_002993"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9396385.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            796..>1131
                     /locus_tag="ACHBFA_002994"
     CDS             796..>1131
                     /locus_tag="ACHBFA_002994"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9396386.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGI"
BASE COUNT          457 a           77 c          202 g          395 t
ORIGIN      
        1 atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat atattgttgc
       61 gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa agttttatgg
      121 aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat aatgatttta
      181 gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt gattttggag
      241 attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat agtgaagaag
      301 aaataggaac aaattttgga gaagatatat taagaatgta tggaaatata gatattgaga
      361 aagcaaaaga atatcaagat atagttgaag aatattatcc tattgaaact attgtttatt
      421 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
      481 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      541 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      601 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      661 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      721 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      781 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      841 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      901 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      961 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
     1021 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
     1081 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat t
//
LOCUS       DAYYNG010000302         1122 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000302 DAYYNG010000000
VERSION     DAYYNG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.312"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1035)
                     /locus_tag="ACHBFA_002995"
     CDS             complement(<1..1035)
                     /locus_tag="ACHBFA_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM9396387.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERL"
BASE COUNT          330 a          244 c          187 g          361 t
ORIGIN      
        1 caaccgttcc aacacattgg ttgtacgaat ccttcggcgg atattttcag gaaaagacaa
       61 aatttgaata gcatcttcaa agccgttttc taaaatgtcg caagcagcac ttaattttga
      121 atcacaacca tatttttcca agaagtgctc ttttctttca cgtgtcaatt caagttcaga
      181 ggctttaaag atactcttca actcgtcttt tacatcagaa gaaactttct ttggcagctt
      241 atcaaaaata tttctcaaaa agtgggcttg gcatctttgc cagctcgcac ctaaaaagtt
      301 cttgcgaaca gccttaacta accctttgtg tgcatcagaa atgaccagtt ttacaccgaa
      361 caaacctcgt tgaacgaacg attgaaaaac ggtatcccaa ttatcttccg attcgccgtc
      421 ttgaacatca aaaccaagaa tcgtacgtcg tccatcagaa tcaatgccga taccaatata
      481 tacacctttt gagacaatac gatgattttc ccttactttc atatacaggg catcacacat
      541 gaaaaatgga tattccgagc cttctaggct ccggtttctc cactcaaaaa caaatggatc
      601 caactgtttc atcagatttg aaacgaatga tttagaaaca gtcgcaccat ccgttagcag
      661 ttcaactgtt ttagttactt tgcgagtgga gacaccagaa atgaccattt caatcatggc
      721 agcgatcaaa gctttttcgc ttcgctgata tctttcaaag atagtaggac taaacttacc
      781 gtctctggtt cgtggaacat gaagttctaa cgtaccaatt cgagttgtaa acgaacgatc
      841 gtagtaacca tttctttgtg attgccgttc ttctgaacgt tcataggcat ttgcctgaat
      901 atattgattt cgttgttctt ccatcagctg attaaacatg atagtcataa gtgtttttgc
      961 ggtatcattc gcaccgcttt cgtttataat tgcttgaatt tcttcgcttt caaaagtaaa
     1021 atgtacttga gtcattgata tcagtcctat tcgagtatgt tttctgcaaa aaacattgta
     1081 ctcctaactg agatcaatgg cttttttgat gcttcacttt ta
//
LOCUS       DAYYNG010000303         1064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000303 DAYYNG010000000
VERSION     DAYYNG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.314"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..700
                     /locus_tag="ACHBFA_002996"
     CDS             134..700
                     /locus_tag="ACHBFA_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9396388.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            845..932
                     /locus_tag="ACHBFA_002997"
     tRNA            845..932
                     /locus_tag="ACHBFA_002997"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:877..879,aa:Ser,seq:cga)
BASE COUNT          381 a          161 c          212 g          310 t
ORIGIN      
        1 ttgcaaaaaa aagttaaaga agactgaaaa aaaatttaaa atataacgtt ttgagtaaaa
       61 tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc agtaaagaag
      121 tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa aagctgctta
      181 tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg ctaaaaagat
      241 ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt taaaaaatac
      301 gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg aacgtactgg
      361 tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc atcatagtct
      421 ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg atttttcata
      481 caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat cggatgaata
      541 tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt cattgatgtc
      601 atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac ggacaattga
      661 ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca gtatgaattt
      721 gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa aaagcgattt
      781 ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta aaaatagtac
      841 ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa ccggctaata
      901 ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa agattagatg ttgtgaagaa
      961 gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct attttttcgt
     1021 ttgattgggc ttattaccgc agatgcaagc ttttgaacac catt
//
LOCUS       DAYYNG010000304         1061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000304 DAYYNG010000000
VERSION     DAYYNG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.315"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>1061)
                     /locus_tag="ACHBFA_002998"
     CDS             complement(36..>1061)
                     /locus_tag="ACHBFA_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9396389.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DAYYNG010000305         1043 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000305 DAYYNG010000000
VERSION     DAYYNG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.316"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACHBFA_002999"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACHBFA_002999"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9396390.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          373 a          132 c          182 g          356 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aac
//
LOCUS       DAYYNG010000306         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000306 DAYYNG010000000
VERSION     DAYYNG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.317"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHBFA_003000"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHBFA_003000"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9396391.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          275 a          227 c          159 g          374 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accac
//
LOCUS       DAYYNG010000307         1026 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000307 DAYYNG010000000
VERSION     DAYYNG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.318"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..828
                     /locus_tag="ACHBFA_003001"
     CDS             88..828
                     /locus_tag="ACHBFA_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396392.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          379 a          182 c          199 g          266 t
ORIGIN      
        1 tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt attgagaaaa
       61 gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt
      121 cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg agtattattt
      181 gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca gatgaaagaa
      241 tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga
      301 ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt accggattat
      361 accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt tggtgattcc
      421 tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac acgaaacgca
      481 tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt acccgcatcg
      541 attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga atcgatgaaa
      601 aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca agaaaaaaga
      661 aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga aacaaagcag
      721 tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt acaaggattg
      781 atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt gatatacaaa
      841 tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt tatactctat
      901 ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt ttctcccaat
      961 aagtaagaaa ataatggcaa aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa
     1021 tagttt
//
LOCUS       DAYYNG010000308         1013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000308 DAYYNG010000000
VERSION     DAYYNG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.319"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            311..>1013
                     /locus_tag="ACHBFA_003002"
     CDS             311..>1013
                     /locus_tag="ACHBFA_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9396393.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKAVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIF"
BASE COUNT          372 a          123 c          179 g          339 t
ORIGIN      
        1 acagacatag tcttgaataa tttttataaa tgttttgatg cttatcaact tattcaaccc
       61 gctatccact tcaagtataa tttttccata cgccaataaa gtagttttag gagcatgttc
      121 tcatttgaga acgctccttt ttttatatat tttgaaagct acttactata cgcgtagttt
      181 caaatagttt tgtcattcta atcttcagca acagaaaaaa atctaaatta taaaatttag
      241 ggttgactaa aatataattt ttgtatacca tatgatcgtg ataatcatta tcatttagaa
      301 ggagaaagtt atgttagaag tgaaaaagct taatgtttgt tataacgatt ttttagcatt
      361 gaacgagatt tctttaaaaa tacaagaagg tgctttaaca ggtattattg gtccaaatgg
      421 tgctggaaaa tctacattgc ttaaagcaat gttaaatatt atccctcatc agggagaggt
      481 ctccattaat aatgaagata tcaataaaaa actggtgaag attgcatatg tagagcaaaa
      541 agctgatatt gattttactt ttccaataaa agtaaaagaa tgtgtatcta tgggaacata
      601 tgccggaatg aaagtttttc aaagaattaa aaatgcagaa tggcaaagag tttcaaaggc
      661 tttggaaaag gtcgatatgg gaaaatattc taatcatcaa ataggagaat tatctggagg
      721 acaatttcaa cgtgtacttt tagcaagatg tcttgctcaa aatgcggatt tcatattttt
      781 agatgaacca tttgtaggga tagacttagt tagtgaacga ataattatgg atacgttaaa
      841 agaattgaaa catcagggaa aagcagtgct aattgtgcat catgatttaa gcaaggtaaa
      901 gaagtatttt gataatatta ttcttttgaa tcgaaatcta attgctcacg gaagtgtaga
      961 atctgtattt aatgaagaga atttaaaaaa agcctatgga gatactatat tta
//
LOCUS       DAYYNG010000309         1005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000309 DAYYNG010000000
VERSION     DAYYNG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.320"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..971)
                     /locus_tag="ACHBFA_003003"
     CDS             complement(24..971)
                     /locus_tag="ACHBFA_003003"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9396394.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT          308 a          221 c          189 g          287 t
ORIGIN      
        1 atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg cttcactttt
       61 tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat tttcccaata
      121 tatttcccga tgattccaag acataacaat tggattccac caatgaacag gaagatcgag
      181 accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa aataatgaac
      241 aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc aatcgttaac
      301 ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt ccagaaggac
      361 caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc tgttttaaat
      421 ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc taggacggca
      481 tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat ttctgtatcg
      541 ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat cgcaggctcg
      601 ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt gatccggttg
      661 atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac ggtcactaaa
      721 tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa atttctggaa
      781 aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc tgctaacgta
      841 ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc tttgaatttt
      901 tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg gacaacaatt
      961 gatactttca tcgtttctat cctttcaatc gtatgtttat atatg
//
LOCUS       DAYYNG010000310          988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000310 DAYYNG010000000
VERSION     DAYYNG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.321"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..>988
                     /locus_tag="ACHBFA_003004"
     CDS             81..>988
                     /locus_tag="ACHBFA_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFM9396395.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYI"
BASE COUNT          367 a          179 c          179 g          263 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatcttta ccaccattcc taaaaaaaat tcaaaatctt
      901 tcagagaagc tgttaaagga atttttaagt tcacagatat taacttagcg cgtgaggcta
      961 aaaatcgatt gattcatgat tatatcga
//
LOCUS       DAYYNG010000311          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000311 DAYYNG010000000
VERSION     DAYYNG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.322"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHBFA_003005"
     CDS             <1..>986
                     /locus_tag="ACHBFA_003005"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9396396.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYNG010000312          982 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000312 DAYYNG010000000
VERSION     DAYYNG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.323"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>982
                     /locus_tag="ACHBFA_003006"
     rRNA            <1..>982
                     /locus_tag="ACHBFA_003006"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          261 a          225 c          299 g          197 t
ORIGIN      
        1 gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc ggcccgtacc
       61 gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa ctctcgttaa
      121 ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat acgatcagcc
      181 gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa aatcgtaaga
      241 tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg cttagcgcaa
      301 gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg tcctaaggta
      361 gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat ttgggcactg
      421 tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta cccgcgacag
      481 gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt accgcatgta
      541 caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg ctggtgggat
      601 actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg agacagtgtc
      661 agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg cccaaaggtt
      721 ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc ttgactgcga
      781 gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt ccgcatggaa
      841 gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct cccccaagag
      901 tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct ggggctgtag
      961 tcggtcccaa gggttgggct gt
//
LOCUS       DAYYNG010000313          947 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000313 DAYYNG010000000
VERSION     DAYYNG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.324"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..750
                     /locus_tag="ACHBFA_003007"
     CDS             <1..750
                     /locus_tag="ACHBFA_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HFM9396397.1"
                     /translation="AHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KAKKPMAFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(784..899)
                     /gene="rrf"
                     /locus_tag="ACHBFA_003008"
     rRNA            complement(784..899)
                     /gene="rrf"
                     /locus_tag="ACHBFA_003008"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          301 a          191 c          172 g          283 t
ORIGIN      
        1 gcgcatactt cctgtgaaga taatggtttt atcttatcta cattaatcac accaggaaat
       61 gtacatgaca gccaagtcgc aatcgatctg gtcaaacagt cgaaaagagc ttttccaaat
      121 ataaagcgtg ttgtcgctga tgcaggatat aagacgccta aatttgttca ttttctgtca
      181 cacttgaaac tctacccgat tcttccatat actgtacctc atggagtaaa aggaatgttt
      241 cgtaaaaaag actttgttta tgatgcctat tttgactgct atttatgtcc caataatcaa
      301 ccgttgttat tttcaactat cactcgtgat ggttatcgaa cctataaatc aaatccaaaa
      361 ttctgtagga gttgtccatt acttaaaaac tgtactttga gtacaaaaca tcaaaaaata
      421 gtaactcgac atgtttgggc ggatcttatg gatgaacttg aacaccaacg ccatacggcg
      481 ttgaatagag aaatttataa aaagagaaaa caaaccattg aacgaatatt tgcagatgca
      541 aaagaaaagc atggtatgcg ttggacgaaa tatcgaggtc tcgaaaaagt cgccacgcac
      601 acgatgctta cgtttgctgc catgaattta aaaaaactag ccacatggct atggaaagca
      661 aaaaagccta tggcttttta tttaaaattt ctacaaagac taacaaaaag gagttggcaa
      721 ttaagccact cctttttgtc tacagtctga gatgaactat ttctagttca tctttttgcc
      781 tttgcgtggc gacgtcctac tctcacaagg ggcaacccct cactacaatc ggcgctaaga
      841 agcttaactt ctgtgttcgg catggttaca ggtgtatcct tctcgctatc gccaccacac
      901 tgtggtgtta tcttttattg agtaaatttt gttcactcaa aactgga
//
LOCUS       DAYYNG010000314          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000314 DAYYNG010000000
VERSION     DAYYNG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.325"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHBFA_003009"
     rRNA            <1..>943
                     /locus_tag="ACHBFA_003009"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYNG010000315          929 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000315 DAYYNG010000000
VERSION     DAYYNG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.326"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACHBFA_003010"
     CDS             <1..355
                     /locus_tag="ACHBFA_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396398.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..929
                     /locus_tag="ACHBFA_003011"
                     /pseudo
     CDS             615..929
                     /locus_tag="ACHBFA_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          356 a          108 c          196 g          269 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagttt
//
LOCUS       DAYYNG010000316          892 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000316 DAYYNG010000000
VERSION     DAYYNG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.327"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..308
                     /locus_tag="ACHBFA_003012"
     CDS             135..308
                     /locus_tag="ACHBFA_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396399.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          298 a          144 c          109 g          341 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gt
//
LOCUS       DAYYNG010000317          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000317 DAYYNG010000000
VERSION     DAYYNG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.328"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACHBFA_003013"
     CDS             <1..888
                     /locus_tag="ACHBFA_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM9396400.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAYYNG010000318          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000318 DAYYNG010000000
VERSION     DAYYNG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.329"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHBFA_003014"
     CDS             <1..672
                     /locus_tag="ACHBFA_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9396401.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAYYNG010000319          784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000319 DAYYNG010000000
VERSION     DAYYNG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.330"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..509)
                     /locus_tag="ACHBFA_003015"
     CDS             complement(<1..509)
                     /locus_tag="ACHBFA_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396402.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNE"
BASE COUNT          261 a          136 c           80 g          307 t
ORIGIN      
        1 ttttcattaa cagtatatat ttgtcctgct aaatatactt ctcccagata tgtgtgttct
       61 tttggtttaa atgtttcaaa taaatgaatc tttacaaaat tactttttga attggctaaa
      121 actttatttt gattcttatc caatacttga tcccctttta gccccatacc ggtatattgt
      181 aaaataccat ttaaccactt atctttataa acattacttc ctgtttgatc actaaacagt
      241 acaagagtat tagtttgatg agatcttctc attccaccct gtttggaaca tttaaacttc
      301 tctgaaattt ctttgttagt atatactcta ccaacttcta actcacctaa tatgttttta
      361 gaccaatttt ctcgccatgt aataatctta tcaagattaa acatttctcc ctcttttggc
      421 atgccatttc taattaaatt agctaataca ccagtagaaa tatctaattt ttttagtaat
      481 tctttcttag taatcaatgt tggattcata acaatgcagc tcctaatgat atcaatataa
      541 ttgcattaga taacttgaaa aatcgaaaaa aactcatttt gtgtcaagtt atctgtaata
      601 tataaaaata tcattattat acctcatcta tgtgaatttt tactattctt accttagcat
      661 attctttata ggtatatcag agctaatcaa actaaaaaat acccgaagtc ttcttatgta
      721 aaaacaccgg ttattactta aatatgactt aggtaaaaca atgagtgatc caattataaa
      781 attt
//
LOCUS       DAYYNG010000320          781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000320 DAYYNG010000000
VERSION     DAYYNG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.331"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..737
                     /locus_tag="ACHBFA_003016"
     CDS             <1..737
                     /locus_tag="ACHBFA_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9396403.1"
                     /translation="EVGELKQQINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGA
                     SHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENL
                     NNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNA
                     VKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSN
                     ILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          347 a           53 c          110 g          271 t
ORIGIN      
        1 atgaagttgg agaattaaaa caacaaatta atgatttgta ttctacttta ttaagaacaa
       61 ttgacgattt ggaatttaaa aataaagaaa ttttaaaatt agaaaagtta aaatatgact
      121 tttttaaagg tgcatcccat gaacttaaaa cccctcttgc tagtcttaaa ataatactgg
      181 aaaacatgaa atacaatatt ggaaaatata aagaaagaga tatttatatt aatgaatgta
      241 ttgaaatcgt tgatagttta acaaaaaata tttctcaaat attatcagtt cattctatag
      301 aaaatttgaa taatgatgaa gaatatttga aaataaatga tacattagaa gacatattaa
      361 aaaagtatga aatattagca aatcaaaaga aaataactgt caataattat atattagacg
      421 agaatgttta tataggaaaa acagctttaa agattattct atctaatttg attagtaatg
      481 cggtaaaata tactgatgta aatggggtaa ttaatattgg gatagttaat gattggttat
      541 atatagagaa ttcatacggt aacaataaaa tttcgaatat ggataaaata tttgatgtta
      601 aatttgattt aaataaggaa aatagtaatg gattaggttt atatattgta agtaatattc
      661 tgaataatta caatatagaa tataaagtat tgcaggatga agaatttttt atctttaaaa
      721 taaaaatatt ttcttaatta agatataatt ttgcaatagt tattgaaaaa tagagactta
      781 t
//
LOCUS       DAYYNG010000321          781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000321 DAYYNG010000000
VERSION     DAYYNG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.332"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..293
                     /locus_tag="ACHBFA_003017"
     CDS             <1..293
                     /locus_tag="ACHBFA_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396404.1"
                     /translation="VNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNTDLKRLFQI
                     SDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLRSL"
     gene            397..>781
                     /locus_tag="ACHBFA_003018"
     CDS             397..>781
                     /locus_tag="ACHBFA_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9396405.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVG"
BASE COUNT          272 a          113 c          165 g          231 t
ORIGIN      
        1 atgttaattc tctggaaata gagttctatt caattttaca aaaaaaactg gaagattaca
       61 cggaaaaatt gcttgcagtc agtcatttac ctctggtatt aacaaatact gatttaaagc
      121 gactatttca gataagtgat agtacactga accgtttagt aaaacttggt gagtttcctc
      181 cctgttggta cgggattcga ggacactatt ttcgtgatga tgttttagaa tggatgagaa
      241 agagtgattc gaatgactat cgagaaaaat tgcgactact tcgatcttta taagttacat
      301 tttctttcta agatgcggta caatgatgac atagatagaa agatttccaa agggtttctg
      361 cttgttgggt tccttcaaaa taggaggaat caataaatgg aaaaaacaga tcgattcaag
      421 aagcaaatca gttctactgg taggatcaca tggtactttc aagcatatcg aacaacaaga
      481 cgtggcttta attcaaaacg tgaggcgcaa ctcgcttatc tggaaatgga gaaacaacaa
      541 aggcttaaga aaaaaattgt tgcaagcaat gacaagttta gtgttgtagg tgagcaatgg
      601 ttccaatatt accgttcgtt aaatgaacaa aaggaaataa cctatgataa gcgagaggaa
      661 caattaaaaa ttattgttcg atggattggt gataaaaagt tgtctgattt gacaccagag
      721 tgccttcaag aattgctttt taccttaaag gaatgtggcg ttaatggaac aaatgttggc
      781 t
//
LOCUS       DAYYNG010000322          765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000322 DAYYNG010000000
VERSION     DAYYNG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.333"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACHBFA_003019"
     CDS             140..460
                     /locus_tag="ACHBFA_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396406.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT          257 a          130 c          140 g          238 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactc
//
LOCUS       DAYYNG010000323          761 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000323 DAYYNG010000000
VERSION     DAYYNG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.334"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..>761
                     /locus_tag="ACHBFA_003020"
     CDS             84..>761
                     /locus_tag="ACHBFA_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396407.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKN"
BASE COUNT          316 a          114 c          110 g          221 t
ORIGIN      
        1 tccagataaa aagtaaatac ttttaaatac ttttatttta taaatatgat atactatctt
       61 taaaaataaa aaggagaaaa aagatgaagc caaatccttc agaagcagga aaacgaatca
      121 aacaattacg cctttcatgt ggttttacta tggaagaact tggaaggaaa atagataatt
      181 caccacgtgc gaccatatcc aactgggaac gtgggaccaa tttaccaaat ccacaaaagc
      241 tgaaattatt atcaactatc actaattcaa caattgactg gattaaatgg ggaactttag
      301 aagaatatat aacttcatac cttatcgata tagggtatga gctatatatt aaggacttcc
      361 cagaaatacc tcacaaagtc tttaaagaca ttcaagaaag atatagtgat acgttttcgc
      421 tagataaaga ctatgagtta ttgaatccta taataaaaaa tatatttaca aaatattact
      481 caaaagattt tgaaatttat ctaaatgaga tacttgctaa taatatcaat cctctgattg
      541 aagagtacgc taaccaaaga cactctatct ctaaagagct atttttcaaa agagccaaca
      601 gtaaacttca agacaagata agaaatggtg atttcaaata cggtgaaaaa gaaagaatga
      661 ttgattcttt aaaacagcta ttagacgaaa tggacttagc ctataaagat atttcaaagt
      721 attcatctgt cgaggatttc ttttataaaa ttactaaaaa c
//
LOCUS       DAYYNG010000324          741 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000324 DAYYNG010000000
VERSION     DAYYNG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.335"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>741
                     /locus_tag="ACHBFA_003021"
     CDS             <1..>741
                     /locus_tag="ACHBFA_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9396408.1"
                     /translation="NRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGVSKITI
                     RKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTVLHSKI
                     IKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSL
                     YKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYS
                     QSTVRCDRISYVIEVTENITLDTVHKNDS"
BASE COUNT          279 a          103 c          129 g          230 t
ORIGIN      
        1 taatagacca ttatatctac aagtagaagc ttctatacgt aacgatattc gtgaaaaaaa
       61 atatcttcca ggagagcaat tacctacaga agaagaatta tgtaaaattt atggagtaag
      121 taagattacc attcgaaaag cttttaaact tttaacagaa agtggaacag ttgaacgatt
      181 aagggggaaa gggacctttg taactcaaaa aaaagaaagt ttaaaattag ctggagttca
      241 tggatttagc gattctcttt ctacccgagg acataaaatt agatatactg ttttacattc
      301 taaaattata aaggctgatg agtttttagc taataaatta caaatcaatg taactgatag
      361 agtttacaat attaaacgtt taatgtggga agacggtgcc ccgatgggaa ttgataattt
      421 tttcgcatca gaagaaaaat ttccaacttt atttgaaaaa ggtagccaat atacttcatt
      481 atataaactt ctagaagatg actatgatgt caaagctacg agctctatta tggaaataaa
      541 cggtttatct gcaacacctg agctttccga cttattgcaa tgtatagtag gagatccgct
      601 atttatttta gaaaaggttg gcaaagataa aaataatcaa ccatttcatt attctcaatc
      661 cacagttcgt tgtgatagaa ttagttatgt tattgaagtc acagaaaata tcactttaga
      721 tacggtgcat aaaaatgatt c
//
LOCUS       DAYYNG010000325          740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000325 DAYYNG010000000
VERSION     DAYYNG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.336"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>740)
                     /locus_tag="ACHBFA_003022"
     rRNA            complement(<1..>740)
                     /locus_tag="ACHBFA_003022"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          146 a          240 c          163 g          191 t
ORIGIN      
        1 cttgtgcggg cccccgtcaa ttcctttgag tttcaacctt gcggtcgtac tccccaggcg
       61 gagtgcttaa tgcgttagct gcagcactga agggcggaaa ccctccaaca cttagcactc
      121 atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac gctttcgagc
      181 ctcagcgtca gttacagacc agagagccgc cttcgccact ggtgttcctc catatatcta
      241 cgcatttcac cgctacacat ggaattccac tctcctcttc tgcactcaag tctcccagtt
      301 tccaatgacc ctccccggtt gagccggggg ctttcacatc agacttaaga aaccgcctgc
      361 gctcgcttta cgcccaataa atccggacaa cgcttgccac ctacgtatta ccgcggctgc
      421 tggcacgtag ttagccgtgg ctttctggtt agataccgtc aagggatgaa cagttactct
      481 catccttgtt cttctctaac aacagagttt tacgatccga aaaccttctt cactcacgcg
      541 gcgttgctcg gtcagacttt cgtccattgc cgaagattcc ctactgctgc ctcccgtagg
      601 agtttgggcc gtgtctcagt cccaatgtgg ccgatcaccc tctcaggtcg gctatgcatc
      661 gtggccttgg tgagccgtta cctcaccaac tagctaatgc accgcgggtc catccatcag
      721 cgacacccga aagcgccttt
//
LOCUS       DAYYNG010000326          739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000326 DAYYNG010000000
VERSION     DAYYNG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.337"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..546)
                     /locus_tag="ACHBFA_003023"
     CDS             complement(<1..546)
                     /locus_tag="ACHBFA_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9396409.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLP"
BASE COUNT          265 a          140 c          132 g          202 t
ORIGIN      
        1 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
       61 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
      121 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
      181 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
      241 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
      301 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
      361 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
      421 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
      481 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
      541 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaataaaa aacgtctttt
      601 gtttcataaa aagtggtttt gacatcaaaa ccgtcatgat ctcctaaaaa aatcttcgtt
      661 ttccaagaac cttaccaaaa aaagtgacag ttttgatacg aaaagtactt tttatgacaa
      721 gtttgtgctt tttcgagtg
//
LOCUS       DAYYNG010000327          737 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000327 DAYYNG010000000
VERSION     DAYYNG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.338"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>737)
                     /locus_tag="ACHBFA_003024"
     CDS             complement(<1..>737)
                     /locus_tag="ACHBFA_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFM9396410.1"
                     /translation="HTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVV
                     ADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLA
                     FSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDL
                     NKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWK
                     GKEPLFFCSKIRNEVDKKLFQARVTSL"
BASE COUNT          206 a          151 c          138 g          242 t
ORIGIN      
        1 ccaaactcgt aactcgagct tggaacagct ttttatctac ttcgtttcga atttttgaac
       61 aaaaaaataa aggctccttg cctttccata gccaggtggc taatttcttt aaattcattg
      121 cagcaaacac tagcatcgta tgcgtcgcga ctttttccaa gcctcgatac ttggtccaac
      181 gcatcccatg cttttctttt gcatccgcaa atattcgttc aatcgtttgt ttccgttttt
      241 tgtaaattga tttattcaga tctgttaggc gaagatgttc cacttcatcc atcagatctc
      301 cccaaacatg tctcgtgatg actcgttgat gatttttact tatggtacat tggtttagca
      361 gaggacagtt cacacattct tttggattgg atttgtattc tcggtaccct tctcgagtaa
      421 ccgttgaaaa agcgagcatt tgatcttgcg gacaaatata acaatcaaaa tactcgtcat
      481 agagaaactc attttttgat aatagctcct ttttcccttt gggccttgaa taaggcaaac
      541 aaggtcgaag gttcaaatga gtcaaaaagt gaacaaactt aggtgtctta tacccagcat
      601 cagcgacaac acaattgatt tcaggaaaaa gtctcttcga ttgtttgacg agctgaaagg
      661 cgacttgact gtcatgaata tttctaggag tgatgattgt gctaagaata aatccattgt
      721 cgtcgcatga agtatgg
//
LOCUS       DAYYNG010000328          725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000328 DAYYNG010000000
VERSION     DAYYNG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.339"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..606
                     /locus_tag="ACHBFA_003025"
     CDS             <1..606
                     /locus_tag="ACHBFA_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9396411.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          269 a          139 c          127 g          190 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag
      601 aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga
      661 tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca
      721 tcttg
//
LOCUS       DAYYNG010000329          712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000329 DAYYNG010000000
VERSION     DAYYNG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.341"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..706
                     /locus_tag="ACHBFA_003026"
     CDS             26..706
                     /locus_tag="ACHBFA_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM9396412.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          251 a          122 c          144 g          195 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg
      601 catggaagcc attcgaggat tatataagaa aacccgaaaa gaaggcactc tcttcgggtt
      661 ttcggtctgt actgaaatca aggtattatt gggaatccca gcttaaatca ta
//
LOCUS       DAYYNG010000330          671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000330 DAYYNG010000000
VERSION     DAYYNG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.342"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>671)
                     /locus_tag="ACHBFA_003027"
     CDS             complement(44..>671)
                     /locus_tag="ACHBFA_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM9396413.1"
                     /translation="TSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTY
                     VRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGK
                     EFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          189 a          157 c          121 g          204 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtctgta tgaaaaagct tgacgtctgt taaagaatac ggtatccgtc
      421 caaaggcttc ttttaccaat gaggcatctt tcttctcacc acaggaataa ccaataattt
      481 ctcgattaaa caaatcaagt attaagcaga tataatgcca ctttttcccc acgcgaacat
      541 aagtaagatc cgtaacgatg gcttccaatg gctgttcttg tgtaaaggtc cgatctaata
      601 cattcgctgt tttcgcttca ttacaagctg ttcgttgccc tttaaaatga gcgatcgtat
      661 aggtagatgt c
//
LOCUS       DAYYNG010000331          670 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000331 DAYYNG010000000
VERSION     DAYYNG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.343"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACHBFA_003028"
     CDS             154..417
                     /locus_tag="ACHBFA_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9396414.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="ACHBFA_003029"
     CDS             417..>670
                     /locus_tag="ACHBFA_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9396415.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAYYNG010000332          657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000332 DAYYNG010000000
VERSION     DAYYNG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.344"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACHBFA_003030"
     CDS             complement(<1..384)
                     /locus_tag="ACHBFA_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396416.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYK"
BASE COUNT          200 a          119 c           91 g          247 t
ORIGIN      
        1 tttataagcc acagaataac tcacgtgatg ctgtttagca aaattttcta ctgagatata
       61 tttttcctga aagaaagcca ataccatttt catagataaa gaatccgtta tataacgaga
      121 taacagagct ttgctagaac attttccatt aatttccaga catatatcca attcactgac
      181 ggttactcgc aaatttttct ctagatcgaa cttttgtaaa tcatgtttca gcttatcgat
      241 cgtttttttc aagagaaatg tcgatagcga caactcttct tgaacattcg atattgactc
      301 tgataaattt gagcttgctt ctaaatattt aagtaatgaa taacttctgt gatcttcttt
      361 gcttaaaaaa aactcataat tcattagtca tcagctcttt ctacgtatat tactttccat
      421 gcattcacca actagctggg gataaaactg atttcctgga tccgagtcac tgttgtcagc
      481 gattcttgtt tgtataatat acatcgtcct gtcttttttt taacattttg gagagcgttt
      541 tatttggcct ttctcgcgat caaacctgtt attagaatta tatcatttac tagtatgcat
      601 ttttattaat aaaatggtat attttatatt gttataaaaa gagcccaacc atcctac
//
LOCUS       DAYYNG010000333          654 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000333 DAYYNG010000000
VERSION     DAYYNG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.345"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..127
                     /locus_tag="ACHBFA_003031"
     tRNA            38..127
                     /locus_tag="ACHBFA_003031"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            142..213
                     /locus_tag="ACHBFA_003032"
     tRNA            142..213
                     /locus_tag="ACHBFA_003032"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Glu,seq:ttc)
     gene            229..301
                     /locus_tag="ACHBFA_003033"
     tRNA            229..301
                     /locus_tag="ACHBFA_003033"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Val,seq:tac)
     gene            311..383
                     /locus_tag="ACHBFA_003034"
     tRNA            311..383
                     /locus_tag="ACHBFA_003034"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Asp,seq:gtc)
     gene            419..494
                     /locus_tag="ACHBFA_003035"
     tRNA            419..494
                     /locus_tag="ACHBFA_003035"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Phe,seq:gaa)
     gene            496..576
                     /locus_tag="ACHBFA_003036"
     tRNA            496..576
                     /locus_tag="ACHBFA_003036"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Tyr,seq:gta)
     gene            583..653
                     /locus_tag="ACHBFA_003037"
     tRNA            583..653
                     /locus_tag="ACHBFA_003037"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Trp,seq:cca)
BASE COUNT          148 a          151 c          177 g          178 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg ccaaactgtc tcaagggttc gaatccctta
      121 ctctccgtag caaatttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      181 gcggtaacac gggttcgaat cccgtacggg tcattttaat tgaatgttgg aggtttagct
      241 cagctgggag agcatctgcc ttacaagcag agggtcagcg gttcgatccc gttaacctcc
      301 ataatgaact ggttccgtgg tgtaggggtt aacatgcctg cctgtcacgc aggagatcgc
      361 gggttcgatt cccgtcggaa ccgtcgttct tgttattcaa ttaataattt taaaatatgg
      421 cgcggtagct cagttggtag agcaacggat tgaagctccg tgtgtcggca gttcgattct
      481 gtcccgcgcc accatggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt
      541 ccttagggtt cagtggttcg aatccactct cctccatttt ataggggcat agtttaaagg
      601 tagaacagcg gtctccaaaa ccgttagtgt gggttcaatt cctgctgccc ctgc
//
LOCUS       DAYYNG010000334          643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000334 DAYYNG010000000
VERSION     DAYYNG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.346"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..469)
                     /locus_tag="ACHBFA_003038"
     CDS             complement(167..469)
                     /locus_tag="ACHBFA_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9396417.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          222 a          106 c           87 g          228 t
ORIGIN      
        1 attggagtgg tagttaatta gtgacttatt tttgatttta tagcactgat actataaaat
       61 atagatatca tcatattaca caatcttaat actaacttaa aaaatatccc ttttataagt
      121 atggtgataa aatccgttcc gggctacctt ttttaggtag cctactttaa tctttatacc
      181 tttctggatc aacgaaagta tacttcaaat agtcataacg ccgatgatcg ctccgtgcgt
      241 ctggcacatc agtcacgata tcaaacaaaa aatacacatc tttcttcatt ctggtttttg
      301 ttgccggaat tttaaagtag ttcttattag agtaatagag attgattaat aagctatctt
      361 cgattgctaa aaagaaaact tctgaatccc acaccttata aaaatctttg acaaatctat
      421 tcgaaggatc aaatttaaac cataattgtg tttttccttc catcaacata atgttcacct
      481 caaaaagagt atacgaacta atgttctttt tgtaaagtag gaatatcatt gataatatgg
      541 atggaacata tatattaaaa agtgttcttt ttgaatgcta ttttgaatgc cattttcatt
      601 catttaacga aaatctgcga aaataaaaaa tgctcaaacc ctt
//
LOCUS       DAYYNG010000335          640 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000335 DAYYNG010000000
VERSION     DAYYNG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.347"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>640
                     /locus_tag="ACHBFA_003039"
     CDS             51..>640
                     /locus_tag="ACHBFA_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9396418.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGR"
BASE COUNT          220 a          119 c          122 g          179 t
ORIGIN      
        1 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata
       61 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaatg aatcaatctg
      121 ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac gatccataaa gtatatctgt
      181 tcttcaagca aggaaaatca gccttagaat attatcaaca gtataagaaa aacaaatcaa
      241 actgtggtag acgtccgctt gttttacccg aggaacaatc agaatatatt caaagaaagg
      301 ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc agcgtttcct attagttgtt
      361 ctgctcgtac catttatcgt atgttcaaaa aggggctatt tgattcttct gacttaccga
      421 tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg tggaaaacaa actttccgcc
      481 gctctattca tgaacgtgaa aaggattata gccaattctc aaatgagttt ggtcaccttg
      541 aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt aattacctta gttgaacgat
      601 tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca
//
LOCUS       DAYYNG010000336          618 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000336 DAYYNG010000000
VERSION     DAYYNG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.349"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..467
                     /locus_tag="ACHBFA_003040"
                     /pseudo
     CDS             1..467
                     /locus_tag="ACHBFA_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          244 a           85 c          101 g          188 t
ORIGIN      
        1 aataaatcaa gagcaattga ttgaagataa agatatataa ccaggaagaa aatcaaacta
       61 gacatattcg taatttatct aatgctaaat tagaggaaga agcaagaaaa gttagtaatt
      121 ataataaaaa aatggctgca aaatatgtta acgaagagga gttttgcttc tcaaaaacaa
      181 aaatatataa acgtaatccc ttcgtatctg aatatgttaa acgacttgca aaaggtattt
      241 gtcaactttg ccaagaaaaa gggccattta taaaagacgg tgtaccttat ttacattgtc
      301 atcatataga atatttgtcg caaggtggaa aagatgtgat tgaaaactgt atcgcgttat
      361 gtcccaattg tcatgctaga attcatgaac ttgagttacc tgacgataaa gaaaaattgc
      421 tgaagatcgt tgaaaaaaga gaaaatagct tttctaaaat accgtaattt atttttatag
      481 gacaaataag ctaatgtgag ctatgccttc tatataagac taagttctat tataagtaac
      541 gaagtaattc tcgttccttc ctgtagtaat attttttaaa taacctatag gcttcttcaa
      601 aagtacatcc tccagtaa
//
LOCUS       DAYYNG010000337          611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000337 DAYYNG010000000
VERSION     DAYYNG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.350"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>611)
                     /locus_tag="ACHBFA_003041"
     CDS             complement(<1..>611)
                     /locus_tag="ACHBFA_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFM9396419.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTD"
BASE COUNT          199 a           94 c           56 g          262 t
ORIGIN      
        1 atatctgtat aatctaaacc gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac
       61 aaattttgtt cttcttctga catagtagaa taaatttctg gtgttaaaaa agttccttta
      121 atttctttat aacctagtat agataattca tcactaatat acgaatattc aatattagga
      181 atttttacat tagtttctaa atttgtattt aaaaaattat atattgcttt ttcttttgca
      241 taaccttttt tcttattagt actaaatttt gttttaaaaa tgtattcatt attaactaaa
      301 tatgccacac tatcataacc actaccgatt atttcaatac tatctacttt gaaattatca
      361 aagtaatgct caattaaata tttcattgcc ttaacatttg tggcattatc atcatatcta
      421 tattccatta aataacaatc ttcttttttg ccctcgtgta attcatgttc tggcaaatct
      481 tcaataattc taaaaccaga tttttggtat gcccttattg ctcttggatt atttttatga
      541 gggtctaaaa taactgcatt agcatttctt tcttttttca aaaattcaaa aatcaattta
      601 atatatcttg t
//
LOCUS       DAYYNG010000338          603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000338 DAYYNG010000000
VERSION     DAYYNG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.351"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..323
                     /locus_tag="ACHBFA_003042"
     CDS             <1..323
                     /locus_tag="ACHBFA_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396420.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          215 a          106 c           75 g          207 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
      421 ttttattaaa ctttttaagt ttctattcta tcaaatattt aaacaatctc tgcatccgaa
      481 aagactacct cgtatagttt tactaacaat atttttatta gcgtgttcaa atcctgattt
      541 cttgtatcaa ttattttctg ttcactttca ataagaaaaa agtctttact tccatggata
      601 ata
//
LOCUS       DAYYNG010000339          593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000339 DAYYNG010000000
VERSION     DAYYNG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.352"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACHBFA_003043"
     CDS             <1..580
                     /locus_tag="ACHBFA_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9396421.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAYYNG010000340          593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000340 DAYYNG010000000
VERSION     DAYYNG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.353"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(318..581)
                     /locus_tag="ACHBFA_003044"
     CDS             complement(318..581)
                     /locus_tag="ACHBFA_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HFM9396422.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          103 c           79 g          187 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaata ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DAYYNG010000341          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000341 DAYYNG010000000
VERSION     DAYYNG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.354"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..512)
                     /locus_tag="ACHBFA_003045"
     CDS             complement(135..512)
                     /locus_tag="ACHBFA_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396423.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          182 a          104 c          102 g          200 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaa
//
LOCUS       DAYYNG010000342          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000342 DAYYNG010000000
VERSION     DAYYNG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.355"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..>588
                     /locus_tag="ACHBFA_003046"
     CDS             171..>588
                     /locus_tag="ACHBFA_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396424.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEK"
BASE COUNT          219 a           95 c          100 g          174 t
ORIGIN      
        1 tttaaatata atatcacttt aggagagaac gaatttcttt agagaaataa aagttagaat
       61 tttctgactt gatttttaca atgaccttaa ttaaaatcgt tctttctaca tttcgtgatt
      121 aagcaaatat caactaagtc agtagaacca ttcacatagg agggtgaaca ttgaaagatt
      181 tttactcagt aaatgaatta gccgaacaat taggtgttac tacacgttct atcagaaatt
      241 atcttcatga aggaaaacta aaaggaacaa aggttggcgg gcaatggaaa ttttctgaac
      301 gtaatctttt tgagttccta tacggagatc aagcagagga agcagcaaaa gaaatgcagc
      361 gattcatgct aaatgcacca atcacgatgc gtttcaacct tcaatatcgt gattttacag
      421 caatcaatca attccgagaa caattagtcc agtaccataa cgatgtttac gcaaataaaa
      481 aagaccgtct tttacaatac gatctttata aagacaatca tgcggagata ctaatcggtg
      541 gtaacttcaa ttatgtcgta aactttagtc aatggatcaa tgaaaaat
//
LOCUS       DAYYNG010000343          555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000343 DAYYNG010000000
VERSION     DAYYNG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.356"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>555
                     /locus_tag="ACHBFA_003047"
     CDS             73..>555
                     /locus_tag="ACHBFA_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM9396425.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          208 a          102 c           90 g          155 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      481 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      541 tatgtcgcta ttttt
//
LOCUS       DAYYNG010000344          552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000344 DAYYNG010000000
VERSION     DAYYNG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.357"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACHBFA_003048"
     CDS             168..362
                     /locus_tag="ACHBFA_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396426.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          235 a           69 c           88 g          160 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat ac
//
LOCUS       DAYYNG010000345          537 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000345 DAYYNG010000000
VERSION     DAYYNG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.358"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACHBFA_003049"
     CDS             <1..365
                     /locus_tag="ACHBFA_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9396427.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
BASE COUNT          211 a           78 c          117 g          131 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacaga
//
LOCUS       DAYYNG010000346          517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000346 DAYYNG010000000
VERSION     DAYYNG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.359"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..>517
                     /locus_tag="ACHBFA_003050"
     CDS             57..>517
                     /locus_tag="ACHBFA_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9396428.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DAYYNG010000347          517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000347 DAYYNG010000000
VERSION     DAYYNG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.360"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..500)
                     /locus_tag="ACHBFA_003051"
     CDS             complement(201..500)
                     /locus_tag="ACHBFA_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9396429.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          165 a           92 c           63 g          197 t
ORIGIN      
        1 ttcataaaat acacttttaa ttaacattat tagtttatac tagctgtttt tgtgttaatt
       61 aaaggataaa atacaatata aagaaacatc tgtttcttta tttagaataa aaaaatacag
      121 actctgttca aataaatgaa cctagcctgc attatgagat tgactatact ttttaattca
      181 ttgagatgaa taactcgttt ttataaatgc ccaaaagtaa tcggtgccga agccattcct
      241 cgacctaatt ctttttcaaa ttttcctatt gttgatatct ccatataaaa cggtgatact
      301 ggtttagaca attctcccct gattgctaac ggacgtaaac tacgttccaa attatttatg
      361 actttaggat aatactgacc tttttctaag cctgtctttg cgtctatcaa aagtatacgt
      421 tccttttctt tgatctctgg atttaaaacg agatcatata attttagtac catgttttct
      481 atttcttcac ttttagacat cacgatttcc tcccaaa
//
LOCUS       DAYYNG010000348          512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000348 DAYYNG010000000
VERSION     DAYYNG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.361"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..132)
                     /locus_tag="ACHBFA_003052"
     tRNA            complement(59..132)
                     /locus_tag="ACHBFA_003052"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Ile,seq:cat)
     gene            complement(299..>512)
                     /locus_tag="ACHBFA_003053"
     CDS             complement(299..>512)
                     /locus_tag="ACHBFA_003053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396430.1"
                     /translation="NHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLN
                     VFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT          175 a           83 c           97 g          157 t
ORIGIN      
        1 gcttgtataa tactcttcaa aaaattctaa cacagaacga ttcaattggc tacattaatg
       61 taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc tgaccaactg
      121 agctaagggt agtggttgtt gccacataaa gccataaaca atcaaccagt agaatgcgtg
      181 gcaacaaacc tgttatcgca tatcttggag tgtgactatt tatgagtgat agtgaagata
      241 tgcgtctcca tatatgtatg gttgagacag ataaacatgt atgaaaaacg ttgttaaatc
      301 aacgtttttt tattttggtc aaggagagaa aagtagagaa atacaaaatt ttttgcattg
      361 attttgcatt caaaaaaaca ttcaaacatt tagaaatccc caatacgtat tcaggaaaat
      421 ctattttaag gtttacaaaa agaacttttg ttcgtacact ctttttgagg tgaactttta
      481 tgctgatgga aggaaaaaca caattatggt tt
//
LOCUS       DAYYNG010000349          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000349 DAYYNG010000000
VERSION     DAYYNG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.362"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHBFA_003054"
     CDS             complement(<1..132)
                     /locus_tag="ACHBFA_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9396431.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAYYNG010000350          480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000350 DAYYNG010000000
VERSION     DAYYNG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.364"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..470
                     /locus_tag="ACHBFA_003055"
     CDS             <1..470
                     /locus_tag="ACHBFA_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9396432.1"
                     /translation="FFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTG
                     TKLMESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDL
                     ENASFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          169 a           66 c           81 g          164 t
ORIGIN      
        1 cattttttgc ttccaatgat tttcaaagaa tggagattac cgactgtata ttcgaaaact
       61 gtgatttttc aggctgtata ttgacaaaaa gcatgttgca tcgcgtgaaa tttgtcaatt
      121 gtaaattaac aggtactaaa ttgatggaaa gttatattgg gaacacgtta ttcgagaatt
      181 gtaaaatgga ctatgtaaat ttatctggtt caaacataaa agaaagtacc tttgagcact
      241 ctgtcttatc aagcgcagat tttgtagact gttccctcac taaaataatg ttttctacta
      301 atgatttaga aaatgctagc ttttttgata ctgatttaaa aaatatcgat ttaagtagca
      361 actcctttga aatgattgaa gtacgctcgg aacatctaaa aaattgtacg cttaatcagt
      421 tgcaagcact tggttttgct agaaaatttt tacaaataaa agtagtataa tcaaaatgaa
//
LOCUS       DAYYNG010000351          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000351 DAYYNG010000000
VERSION     DAYYNG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.367"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHBFA_003056"
     CDS             <1..>468
                     /locus_tag="ACHBFA_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM9396433.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAYYNG010000352          454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000352 DAYYNG010000000
VERSION     DAYYNG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.368"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..>454
                     /locus_tag="ACHBFA_003057"
     CDS             43..>454
                     /locus_tag="ACHBFA_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9396434.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKW"
BASE COUNT          173 a           54 c           98 g          129 t
ORIGIN      
        1 atttgtaaaa ataaaaaaga tgatttttag gaagtgaaga gagtgcataa aattagtgtt
       61 atagtacccg catataacgt agaaaactat ttagaaaact gcttagaatc tatttccaat
      121 cagacacatt caaatattga aataatagta gtcgatgatg gttccccgga tcgaagtggg
      181 gaaattgcag aagaatataa aaagaaagat atgcgattaa aagtgataca tcaagtgaat
      241 ggtggtttaa gtgcggctag gaattctggt cttgattctg caacaggaga ttttgtgtgc
      301 tttattgaca gcgatgattg gatagaacca gactatcttg agttactata ttttggtacg
      361 caaacttatg gagcagatat ctcggtgcta aaaataaaaa agatttcaag tttagaacaa
      421 ctaaaagaac atacaattgc tgagagaaaa tggg
//
LOCUS       DAYYNG010000353          449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000353 DAYYNG010000000
VERSION     DAYYNG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.369"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>449
                     /locus_tag="ACHBFA_003058"
     rRNA            <1..>449
                     /locus_tag="ACHBFA_003058"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          105 a          120 c          122 g          102 t
ORIGIN      
        1 accaggtctt gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt
       61 gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa
      121 cgagcgcaac ccttattgtt agttgccatc attcagttgg gcactctagc aagactgccg
      181 gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggc
      241 tacacacgtg ctacaatggg aagtacaacg agttgcgaag tcgcgaggct aagctaatct
      301 cttaaagctt ctctcagttc ggattgcagg ctgcaactcg cctgcatgaa gccggaatcg
      361 ctagtaatcg cggatcagca cgccgcggtg aatacgttcc cgggccttgt acacaccgcc
      421 cgtcacacca cgagagtttg taacacccg
//
LOCUS       DAYYNG010000354          445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000354 DAYYNG010000000
VERSION     DAYYNG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.371"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..>445
                     /locus_tag="ACHBFA_003059"
     CDS             60..>445
                     /locus_tag="ACHBFA_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396435.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHH"
BASE COUNT          139 a          101 c           95 g          110 t
ORIGIN      
        1 gaagtccctt ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata
       61 tgaacgattt tactacagaa attctaaaga ctctagcgaa caaaggcgat ttgaatgaat
      121 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
      181 tcctcgatta cgaaaagtac gatcgcattg gttttaacac gggtaattct cgtaacggct
      241 cctatgaccg tacggtcaag accgagtacg gggaacttca tctccagatt ccgcgcgacc
      301 gcaacggcga gttcaagcaa cagactgttc ctgcttatag acggacgaat gataccttag
      361 aggagaccgt cattcacctc ttccgaaaag gtattaccat gtcggaaatc gcagacttga
      421 ttgagaaaat gtatgggcat cacta
//
LOCUS       DAYYNG010000355          442 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000355 DAYYNG010000000
VERSION     DAYYNG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.372"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>442
                     /locus_tag="ACHBFA_003060"
     CDS             <1..>442
                     /locus_tag="ACHBFA_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFM9396436.1"
                     /translation="DYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQFYFQT
                     WHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEKYVSAFNIVPSKIL
                     EVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL"
BASE COUNT          167 a           63 c           74 g          138 t
ORIGIN      
        1 agattatatt aaaagaattg attccgattc ttttttagct gccttttatt tgtatacagc
       61 taagtattgg atagacgata gtcggaaaac gattaagtat gcaaaaagga aaaaacaatt
      121 ttattttcaa acatggcacg gtacgccttt aaaaaaaatt gaatttgatg caaaagataa
      181 gttacctaaa cgttacctaa gctatgcgaa aaaagatagt gagtcaatta catatttatt
      241 atctggtaat cgctatagtt ctgagaagta tgtttcagca ttcaatatcg ttccttctaa
      301 aatattagaa gtaggaactc ctagaaacga tgaattagcc tctagtaaag agcaagtgtt
      361 tgacttgaaa aagaaacaaa tcaatgtctt atttgctcca acttttcgaa ataatattga
      421 ggacaacgga attacgcaac ta
//
LOCUS       DAYYNG010000356          431 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000356 DAYYNG010000000
VERSION     DAYYNG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.375"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>431
                     /locus_tag="ACHBFA_003061"
     CDS             52..>431
                     /locus_tag="ACHBFA_003061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9396437.1"
                     /translation="MEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSFNPRIF
                     DSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRSINILQ
                     RFEKEVKIDPDFSPKLKKLVKFLL"
BASE COUNT          166 a           58 c           61 g          146 t
ORIGIN      
        1 ttattcaata gaaatttatt tgataatata agattcccac tgggtaaatt aatggaagat
       61 aaggctacta cttatttgtt gatcgatcat tccgaaaaag ttgctgtcaa ttcatctgct
      121 aaatatcact attaccaaag ccctaaaagt atactgagag gaagcttcaa tcctagaatt
      181 tttgattctt ttgaagtcca tgaagatata ctagtattca tagataagtt ttatccggaa
      241 ttatcaatta atgttagaac tcgctatgtc tatgaagcaa taaggatgat gatgacaatg
      301 attaaaaata actataccaa taaagaagat tatttacgtt ctataaatat tttacaacgc
      361 tttgaaaaag aagtgaaaat tgatcccgac ttttcaccaa aattaaaaaa attagtaaag
      421 tttttattaa t
//
LOCUS       DAYYNG010000357          430 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000357 DAYYNG010000000
VERSION     DAYYNG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.376"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>430
                     /locus_tag="ACHBFA_003062"
     CDS             <1..>430
                     /locus_tag="ACHBFA_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM9396438.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAYYNG010000358          430 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000358 DAYYNG010000000
VERSION     DAYYNG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.377"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..329
                     /locus_tag="ACHBFA_003063"
     CDS             <1..329
                     /locus_tag="ACHBFA_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396439.1"
                     /translation="GKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVE
                     FVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCA
                     NASEAV"
BASE COUNT          131 a           83 c           95 g          121 t
ORIGIN      
        1 gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc
       61 gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt
      121 cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa ttggatttgg
      181 agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca cttggctacg
      241 agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat aatgagctcg
      301 gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc gaagatcgtc
      361 acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag aagttctgca
      421 ccttataaaa
//
LOCUS       DAYYNG010000359          420 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000359 DAYYNG010000000
VERSION     DAYYNG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.378"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..403
                     /locus_tag="ACHBFA_003064"
     CDS             74..403
                     /locus_tag="ACHBFA_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9396440.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DAYYNG010000360          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000360 DAYYNG010000000
VERSION     DAYYNG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.379"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHBFA_003065"
     CDS             complement(<1..339)
                     /locus_tag="ACHBFA_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396441.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAYYNG010000361          413 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000361 DAYYNG010000000
VERSION     DAYYNG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.380"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>413)
                     /locus_tag="ACHBFA_003066"
     CDS             complement(<1..>413)
                     /locus_tag="ACHBFA_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396442.1"
                     /translation="IDAAIQFDFIYPIVESTYSTHGRPSIDPVVLIKLVFIQYLFGIR
                     SMRQTIKDVETNVAYRWFLGYSFEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQA
                     VKAGFVTEDNLYIDSTHIKANANKHKFTKEMTYGQA"
BASE COUNT          143 a           69 c           75 g          126 t
ORIGIN      
        1 gcttgaccgt aggtcatttc ttttgtgaac ttatgtttat tggcatttgc cttaatatga
       61 gtagaatcaa tataaaggtt gtcttccgta acgaagccag cttttacagc ttgttctaga
      121 atatgagaga aaatgtcttc aaataaagtt gtttctctga atcttcgaac atagttttta
      181 ccaaaagtag agaaatgcgg gataggatcc tcgaatgaat aacctaaaaa ccaacgataa
      241 gcaacatttg tttcaacgtc tttaatagtc tgtctcatcg aacggatacc aaataaatat
      301 tggataaata ctaatttaat taagaccact ggatcaatac tgggacgacc atgggtcgaa
      361 tacgtagatt ctacaatggg ataaatgaaa tcaaactgaa tcgcagcatc aat
//
LOCUS       DAYYNG010000362          411 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000362 DAYYNG010000000
VERSION     DAYYNG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.381"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..391
                     /locus_tag="ACHBFA_003067"
     CDS             161..391
                     /locus_tag="ACHBFA_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396443.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT          157 a           53 c           61 g          140 t
ORIGIN      
        1 cattgatata taagcatttt taaaagattt tatatttttg tatgtcattc atatatcaat
       61 agctaactta atatcagtca aattatgccc cttttttgcc cctcaaatat cgctttacaa
      121 caagaacgta agttcgtata atcgttttga ggtgattttt atgatggaag aatttattag
      181 aaaaaatatc agtgatgaat atgcagactt ttatgatcaa agcagtaaaa aagataaatt
      241 ccagatggat gtttcaatct tagctatatt agctttttcc gaaaataaac aacctgtaac
      301 tgcaaaaaaa gaaacagtat tctctgaagg caaaataaaa actcgatata tattagaggt
      361 agaaactaat tttaaaaaca gatcggagta atggttatgc tttttaatga g
//
LOCUS       DAYYNG010000363          407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000363 DAYYNG010000000
VERSION     DAYYNG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.383"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..384
                     /locus_tag="ACHBFA_003068"
     CDS             <1..384
                     /locus_tag="ACHBFA_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396444.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           73 c           64 g          113 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg
       61 aataccttct tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag
      121 tcactcatag aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga
      181 accatttact caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt
      241 agaaaagagc agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat
      301 acatacaatc aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca
      361 ttagtttcaa tgtttactga gtaactaatt attttgcagg aggacaa
//
LOCUS       DAYYNG010000364          396 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000364 DAYYNG010000000
VERSION     DAYYNG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.384"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..>396
                     /locus_tag="ACHBFA_003069"
     CDS             181..>396
                     /locus_tag="ACHBFA_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003137379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396445.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIY"
BASE COUNT          143 a           65 c           65 g          123 t
ORIGIN      
        1 tttatacatg tttatcgtta ttaatagtga ataggaaaaa acctattttt gatcattgac
       61 aattaaatac aatttttgaa taacgatccc tgctattcga gctagaaact caagtacgcc
      121 atgacctgat tagcgacaaa tacttagaga tttctttttg ttctgggaaa acaaaatgcc
      181 atgacaatag cagcaaataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      241 aaattcctat gtgcttaccc aaagcaattc aaaaatttca taactactat tttaggagat
      301 gattttatag aatccagagt ctacctgaat aaaaaacaaa cctgtgcatt tctaggaatc
      361 gataataata ctctagataa gtggattcgt atatat
//
LOCUS       DAYYNG010000365          394 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000365 DAYYNG010000000
VERSION     DAYYNG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.385"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..285
                     /locus_tag="ACHBFA_003070"
     CDS             <1..285
                     /locus_tag="ACHBFA_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9396446.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DAYYNG010000366          384 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000366 DAYYNG010000000
VERSION     DAYYNG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.386"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>384
                     /locus_tag="ACHBFA_003071"
     CDS             <1..>384
                     /locus_tag="ACHBFA_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9396447.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIVC"
BASE COUNT          141 a           69 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tgca
//
LOCUS       DAYYNG010000367          357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000367 DAYYNG010000000
VERSION     DAYYNG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.387"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>357
                     /locus_tag="ACHBFA_003072"
     CDS             <1..>357
                     /locus_tag="ACHBFA_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396448.1"
                     /translation="EVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILS"
BASE COUNT          100 a           70 c           94 g           93 t
ORIGIN      
        1 ggaagtgttg agctatgcga ttgcaccaac tgaatctatc acgatttggg aagagatttt
       61 aatagacctc caggaacgtg gcgtgaaaaa cgtcctcctt ttcatcacgg atggcttaaa
      121 ggggatggca ggggccgtgc agcggttcta ccccaaagcc cgttttcaac attgctgtgt
      181 gcacgtttcc cgtaatatca gtcacaaagt gcgtgtcgat gatcgtaagg aagtctgtga
      241 tgattttaaa atggtgtatc aagcgtcatc taaaaaggcg gcattggaag cacgtggtgc
      301 ttttgcggaa aaatggaaaa ccagttatcc aaaaatggtg gaatcgatcc tttcgaa
//
LOCUS       DAYYNG010000368          351 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000368 DAYYNG010000000
VERSION     DAYYNG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.388"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>351)
                     /locus_tag="ACHBFA_003073"
     CDS             complement(<1..>351)
                     /locus_tag="ACHBFA_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396449.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAE"
BASE COUNT          131 a           58 c           42 g          120 t
ORIGIN      
        1 ttctgctaag cttattattg aaaatactga taacttataa ttatatcgtc cattgacaat
       61 caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt gttcttgatt
      121 agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta tttcttctcc
      181 tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aatgctactg gccactgata
      241 gttcaaggtg gttgctctcc aaccggtttc ccccattact gtcaatggta ttaaacagta
      301 tatagagcaa accaataata aatttttaac atttattttc tcaaaaacat a
//
LOCUS       DAYYNG010000369          340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000369 DAYYNG010000000
VERSION     DAYYNG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.390"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..164
                     /locus_tag="ACHBFA_003074"
     CDS             42..164
                     /locus_tag="ACHBFA_003074"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396450.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            137..268
                     /locus_tag="ACHBFA_003075"
     CDS             137..268
                     /locus_tag="ACHBFA_003075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396451.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          122 a           46 c           64 g          108 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc
//
LOCUS       DAYYNG010000370          326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000370 DAYYNG010000000
VERSION     DAYYNG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.391"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>326
                     /locus_tag="ACHBFA_003076"
     CDS             <1..>326
                     /locus_tag="ACHBFA_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM9396452.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSR"
BASE COUNT          119 a           54 c           62 g           91 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgt
//
LOCUS       DAYYNG010000371          323 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000371 DAYYNG010000000
VERSION     DAYYNG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.392"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBFA_003077"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBFA_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM9396453.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAYYNG010000372          319 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000372 DAYYNG010000000
VERSION     DAYYNG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.393"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>319
                     /locus_tag="ACHBFA_003078"
     CDS             <1..>319
                     /locus_tag="ACHBFA_003078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396454.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDN"
BASE COUNT          137 a           40 c           57 g           85 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagttgga gtatgggaag
      301 caaaaaaaga gagataaca
//
LOCUS       DAYYNG010000373          311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000373 DAYYNG010000000
VERSION     DAYYNG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.394"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..218)
                     /locus_tag="ACHBFA_003079"
     CDS             complement(21..218)
                     /locus_tag="ACHBFA_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9396455.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          100 a           67 c           32 g          112 t
ORIGIN      
        1 gcttagcatt ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct
       61 atagacattc ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta
      121 gtgcaataca ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt
      181 tgtgtctctt taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa
      241 tgtttttaga ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac
      301 gtagcaaaat a
//
LOCUS       DAYYNG010000374          311 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000374 DAYYNG010000000
VERSION     DAYYNG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.395"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..>311
                     /locus_tag="ACHBFA_003080"
     CDS             32..>311
                     /locus_tag="ACHBFA_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM9396456.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT          120 a           36 c           55 g          100 t
ORIGIN      
        1 tgttaatgaa caagatagga agaaacattt aatggacagt tttcagagat tgtcactagt
       61 aaatcaattt gagttattgg ctaaatttga ggaccaaaac agatcttatt atgaaagaaa
      121 aatcgaaata ttgagagaag gttacgagta tcattatgat gatgaaattt ggagtgatct
      181 ttctgagcca tttccgaaag aagattctcg atttgtatta gatgtattga acatgtatcg
      241 agatatcaat tttagtaaat caaagctaaa cttagatgaa aaccaaatta ctaattcata
      301 ctatactcat t
//
LOCUS       DAYYNG010000375          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000375 DAYYNG010000000
VERSION     DAYYNG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.396"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          124 a           44 c           48 g           92 t
ORIGIN      
        1 cacttttttt caaaaatatg gtatgcttct tagtgaattc aaatataaaa gagtttaaag
       61 cgtaatacac ttatggggaa gtggtttggg gtgcgcttta aactcttctt tattattatc
      121 tcacagcatg cttaaaaggt ctatggatta agttatgtaa aaaagtgcct ctttaaacgt
      181 cctaaaatcc aaacaaggat agcataatat acactatcgt tgatccagaa aacaggtaaa
      241 taaattttgc aaaagcaata agttaaaaac attacaaaaa agacaaggat atcacatcaa
      301 gaaaaaat
//
LOCUS       DAYYNG010000376          295 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000376 DAYYNG010000000
VERSION     DAYYNG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.397"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..>295
                     /locus_tag="ACHBFA_003081"
     CDS             76..>295
                     /locus_tag="ACHBFA_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9396457.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT          111 a           57 c           51 g           76 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt tcctg
//
LOCUS       DAYYNG010000377          292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000377 DAYYNG010000000
VERSION     DAYYNG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.399"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>292)
                     /locus_tag="ACHBFA_003082"
     CDS             complement(<1..>292)
                     /locus_tag="ACHBFA_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396458.1"
                     /translation="IERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAVL
                     HFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQ"
BASE COUNT           85 a           64 c           49 g           94 t
ORIGIN      
        1 tttgtttttg cagttctgct gcagtcagat ggatcttccc atcgtttgta tcgatcatta
       61 aaccttctgg aaacagttca accactgctc gatcccaaga ttcaagagaa agctcgatat
      121 aatcttttac tggtaattcc aattctacct taaaaaagtg caaaacagcc gacagccctt
      181 ctgcttgata gatttctcct aatgttagcc cctctagatg gaactctggt gaaaaatgca
      241 gcaatgtttt attttcctgc aattgaacat atagaaatgt agatcgctca at
//
LOCUS       DAYYNG010000378          290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000378 DAYYNG010000000
VERSION     DAYYNG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.400"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..>290
                     /locus_tag="ACHBFA_003083"
     CDS             37..>290
                     /locus_tag="ACHBFA_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396459.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           88 a           53 c           57 g           92 t
ORIGIN      
        1 atagatttat gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat
       61 ggtagaaatc aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt
      121 cgaaagattg atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac
      181 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
      241 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac
//
LOCUS       DAYYNG010000379          281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000379 DAYYNG010000000
VERSION     DAYYNG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.403"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..281
                     /locus_tag="ACHBFA_003084"
                     /pseudo
     CDS             1..281
                     /locus_tag="ACHBFA_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          111 a           56 c           54 g           60 t
ORIGIN      
        1 aaacaacaaa tcgtcgatct ctatctcgct ggtaagcctc gcgcagaaat tattcgagag
       61 tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc aacgggctca
      121 ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact aagaaagaaa
      181 aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat attcggacga
      241 aaagacaagt aattgatgcc aacaagcata aatattccat a
//
LOCUS       DAYYNG010000380          280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000380 DAYYNG010000000
VERSION     DAYYNG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.404"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>280)
                     /locus_tag="ACHBFA_003085"
     CDS             complement(<1..>280)
                     /locus_tag="ACHBFA_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9396460.1"
                     /translation="MRQTIKDVETNVAYRWFLGYSFEDPIPHFSTFGKNYVRRFRETT
                     LFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFTKEMTYGQA"
BASE COUNT           86 a           47 c           52 g           95 t
ORIGIN      
        1 gcttgaccgt aggtcatctc tttcgtgaac ttatgtttat tggcatttgc tttgatatga
       61 gtagaatcaa tataaaggtt gtcttccgta acgaagccag cttttacagc ttgttctaga
      121 atatgagaga aaatatcttc aaataaagtt gtttctctaa atcttcgaac atagttttta
      181 ccaaaagtag agaagtgtgg gataggatcc tcgaatgaat aacctaaaaa ccaacggtaa
      241 gcaacatttg tttcaacgtc tttaattgtc tgtctcatcg
//
LOCUS       DAYYNG010000381          274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000381 DAYYNG010000000
VERSION     DAYYNG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.405"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>274
                     /locus_tag="ACHBFA_003086"
     CDS             <1..>274
                     /locus_tag="ACHBFA_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9396461.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMA"
BASE COUNT           66 a           56 c           78 g           74 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggc
//
LOCUS       DAYYNG010000382          247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000382 DAYYNG010000000
VERSION     DAYYNG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.denovo.407"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>247)
                     /locus_tag="ACHBFA_003087"
     CDS             complement(<1..>247)
                     /locus_tag="ACHBFA_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9396462.1"
                     /translation="ESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQR
                     GLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT           80 a           44 c           40 g           83 t
ORIGIN      
        1 attatttcgt ttagatgcga cagattgaat aaaaaattca tctactgaat tgaaatccat
       61 agatttcaat aaaccatctc tacgtaacaa tccgttagaa ttctcgttta ggcctctttg
      121 agacggcgtt cctggatcag cgaaataaat ggcaatatca ttgacattac tgatctgttt
      181 ccaatttgaa aattcctttc cacaatcaaa agtgatggat ttgaataggt ttttcggtac
      241 agattca
//
LOCUS       DAYYNG010000383         9178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.116, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000383 DAYYNG010000000
VERSION     DAYYNG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.116"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..155
                     /locus_tag="ACHBFA_003088"
     CDS             <1..155
                     /locus_tag="ACHBFA_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9396463.1"
                     /translation="LPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDL
                     SSVENK"
     gene            complement(305..1246)
                     /locus_tag="ACHBFA_003089"
     CDS             complement(305..1246)
                     /locus_tag="ACHBFA_003089"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9396464.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1372..2493
                     /locus_tag="ACHBFA_003090"
     CDS             1372..2493
                     /locus_tag="ACHBFA_003090"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9396465.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2751..4253
                     /gene="eat(A)"
                     /locus_tag="ACHBFA_003091"
     CDS             2751..4253
                     /gene="eat(A)"
                     /locus_tag="ACHBFA_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9396466.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4320..4847
                     /locus_tag="ACHBFA_003092"
     CDS             4320..4847
                     /locus_tag="ACHBFA_003092"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9396467.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5058..6119
                     /locus_tag="ACHBFA_003093"
     CDS             5058..6119
                     /locus_tag="ACHBFA_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9396468.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6122..7018
                     /gene="murQ"
                     /locus_tag="ACHBFA_003094"
     CDS             6122..7018
                     /gene="murQ"
                     /locus_tag="ACHBFA_003094"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9396469.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7032..9014
                     /locus_tag="ACHBFA_003095"
     CDS             7032..9014
                     /locus_tag="ACHBFA_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9396470.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9079..>9178)
                     /locus_tag="ACHBFA_003096"
     CDS             complement(9079..>9178)
                     /locus_tag="ACHBFA_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9396471.1"
                     /translation="QTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3009 a         1640 c         2012 g         2517 t
ORIGIN      
        1 aattaccgga atttctgcat acaccattta agccaatcct atcagagcta ttggaatttt
       61 atgatgcaga aaattgggca aaacgattta tcactaacga ttccactatt tatgagcaat
      121 acgaatatga cctctcctct gttgaaaaca aataaaaaac ctacattttg aagagattat
      181 gtactgaaac aaacaagcac aattcttatg gcatcatgag agttgtgttt tctttttgat
      241 cttttattgg gcttaaaaaa agagaaccag ctgctgattc agctgattct catcagagag
      301 ccttttattc tgcgccaagg attccttctt ttgcatggac taattgggtc aaaaaggcac
      361 aatatggtgt agcaatatca tatttttgtc cttttcgcca aatggcacca ttgatgtagt
      421 cgatttcagt caaacgatgg tttttgatca ggtcttggta catagatggg taatgcaacc
      481 cgatgttgtc aggatcataa gtagaagcaa tatgttggta cacttcttct tgatctagta
      541 tgattccttc ttttgctgcg atagaagcaa attcagatac gattgttcga accatgctct
      601 ctgccgcttt ttgcgcgcct agttcagcaa tattacaatc caaaatcgtg cataaaccat
      661 tcaatgttcc atttacacaa gcttttcgcc aaatggaata acgaacgtta tggctataga
      721 tcggatttaa atttgcttct tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt
      781 ctggttccaa gttctctagt tcgatttctc catcacctaa caatgtgact tttccgggac
      841 cagctagacc agctgtccac atcgtgatcc caaataagat atttttcttt ggcacatatt
      901 tttccagtac atcttcatga cctaatccat ttagtaaaca caaaacataa gtattgtcag
      961 taataatgga ttggatcgaa caaaacatat catctaattg attggctttt gttaatgcta
     1021 cgatcaagtc cacgtgttcg ttgctttcga tgatttcttc tggtgaatag attggcagtt
     1081 ttgcgaccac ttctttaccg ttaaagtcag caatcaaccc atttgtacga atggcttcaa
     1141 tgtgagcagg ccaacgatcg atcaagagga cctcatttcc gctttgatgt agcatcaatc
     1201 cgattcggct gcccattgct ccggccccag ctattgcgat tttcatgttt tttctctcct
     1261 ttattcaatg agttcccttt tagtatatag aaaaatggaa gaaaatagca ttgttttgtt
     1321 tgaaaaaaat gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt
     1381 ttggcggcaa ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt
     1441 ctaaatggct gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga
     1501 gaaaatactg cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg
     1561 atggatctct tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa
     1621 aacaccgcag tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg
     1681 gatcaaacta tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg
     1741 cttcaagaag tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt
     1801 ctaggattac tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta
     1861 ttatctacta aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg
     1921 attatcgctg cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt
     1981 ggaagacaaa aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag
     2041 attgctcagc aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga
     2101 gcagcaggcg gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc
     2161 gatttagtca gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt
     2221 acaggagaag ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca
     2281 gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag
     2341 ctagaaggac tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt
     2401 ttagaagaag caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca
     2461 cttagcagat ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat
     2521 cgtaatttca aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga
     2581 tgaaagctgc attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg
     2641 attggcttgg ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct
     2701 ttagccagtt ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa
     2761 tcgaaataaa aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag
     2821 caaatctaaa ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa
     2881 agacaacttt actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc
     2941 agcaagaatt tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg
     3001 tgttaaatga tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc
     3061 aaacagatcc tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa
     3121 aagtcctact ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa
     3181 cgaatcattt ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac
     3241 aaggcttcat cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt
     3301 tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac
     3361 agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca
     3421 gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata
     3481 aaacaaagaa acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag
     3541 tgggtgctga tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga
     3601 cccagatcag cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga
     3661 tgaatagcca agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt
     3721 atgaaaatct gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga
     3781 tttcaggtcc taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca
     3841 acggcaaggt tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc
     3901 aaaattatga agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact
     3961 accaagcatt tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca
     4021 agatcgagca gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac
     4081 agccagctga actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag
     4141 aacaattaga acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc
     4201 aaaccttcct ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt
     4261 aatctcaaga acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca
     4321 tgctttatgt aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac
     4381 gggcagatgt gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg
     4441 tagtagattt gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag
     4501 atacagcaca agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg
     4561 tagaaatgga ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag
     4621 cacgtttagc gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac
     4681 ggatcgttcc tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt
     4741 gccataatgc cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt
     4801 tctttacatt catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg
     4861 ttatttaaat aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat
     4921 gacaattctt ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa
     4981 aattattgaa attttgtttc tttagatata tactctagtc atagaaatga agacggatac
     5041 agaaaaagag ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag
     5101 agaagaaaca gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca
     5161 tgcatatacc agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg
     5221 aagcgaaaaa gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag
     5281 cgggtttttc attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa
     5341 tggattatca tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt
     5401 tgaatgctag cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt
     5461 tctcacagat tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag
     5521 aatggtatca gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg
     5581 taccaggaga tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac
     5641 atcgagggat ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg
     5701 tttacatcgg agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa
     5761 aagaacagac agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat
     5821 tagggaagca tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac
     5881 gttttcgaga gattccgcct atcccagcaa gaaatacagc tacgcgtatg aaagggtcta
     5941 ttactttaga taacgaaaag tatttgcgct atatgggtga gatccaatta accaaatatg
     6001 atttgccagc ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa gagctaccat
     6061 tgatcaacca aatcaaagct ggtatgaact atcaatttat aagaaaagaa ggaagataaa
     6121 aatgagcaaa atcaatttag agaacctaac aacggaacgc agaaaccaag aaacttttgg
     6181 attagatgag atgagtattt ctcaagccgt tcaactaatg aacaaagaag atcagaaagt
     6241 ggccgctgcg gttgaaaaag aactgacaag tatcgaacca gtgattgcta acacgattga
     6301 gtcctttaaa aaaggtggac gtctgattta tatgggagcc ggaacaagtg gaagactagg
     6361 tgtgttagat gctgctgaat gcgtaccgac attcggtgta gaaccggaaa tggtcatagg
     6421 tctaattgct ggtggagaag aagcaatgac tgtagctgtc gaaggggccg aagattctta
     6481 tgagctaggg caagaagatc taatcaagct gaatttgaca aaaaatgata tagttattgg
     6541 tatcgcagct agcggtcgta caccatatgt gatcggtgga ttagattacg caaaatcgat
     6601 cggtgctgtc acaggaacga tttcttgcaa taaacaagcc gaaatcagta aacacgcgga
     6661 tttcccaatc gaagtcgatg caggtccaga atttttgaca ggctctactc gattgaagtc
     6721 aggaacagca caaaaactta tcttgaatat gatttctact atcagtatga ttggtatagg
     6781 taaagtattc aataacttga tggtcgatgt caaaccaaca aatgaaaaac taatcgaacg
     6841 ctcaaaacga atcatcatgc aagcgacaga agctgattac gaaacagctg aaaagtactt
     6901 tgaagaagca gatcaaaatg ttaagctggc tatcgtcatg attttgacaa atagtgataa
     6961 agaggaagct caagcaaaat tagctgaagc aaacggattt gtaaaaggga ccttatgagg
     7021 gggaaaagac catggcaaaa aacgaactga ctttggaaga actggcaaaa caaatttata
     7081 aaggggcagg cggcatgggc aatgtagaaa gcattgtcca ctgcatgact cgtgtgcgta
     7141 tgaagatcat caatgatgat cttgtaaatg tctctgaatt aaaagcgatt ccaggagtgc
     7201 ttggtgtagt agaagatgaa cagcttcaag tcatcatcgg acctgggaaa gtgaacaaag
     7261 tagcacagca aatggttgat ttggcaggcg tgaaattagg agaaaaactt tcttctgtaa
     7321 gtacagctac agcaaattca tcagcatctg gaaaagaccg tgtaaacgaa cgggcacaag
     7381 aaatgaaagc ggcacaaaaa tcgaaacaaa aaccatctaa aatcaaatcg gtattaaaag
     7441 acatttctaa tatctttgtg ccaatgattc ctgcatttgt cggtactggg atcgtggcag
     7501 ggattgccgc agttttagca aacttagtca cagccggaac acttgatgca gcaacatggc
     7561 aacaatatat cgatgtgatg aacatcttga aaaacgggat gtttagctac ctagttatct
     7621 atacaggtat caatgctgca caagtttttg gtgcaacacc aactcttggt ggagttatcg
     7681 gggccgttgt gatgctgaca gggatgaatc cggaagcacc actgaaaaac ttatttactg
     7741 gtgaagctct tgcagctgga caaggtggga tcctaggtgt catcttcgct gtttggctat
     7801 tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga tgcggtagat atcatcgtta
     7861 caccaactct ttcattgatt gccatcggtc tgattgaaat tttcttcatc atgcctttag
     7921 caggtttcat ttctgatggc atggtcggtg ggatcaactg ggtacttggc gttggtggag
     7981 catttgccgg cttcgttcta ggcacattat tcttgccgat ggttatgttc ggactgcatc
     8041 aaattttgac tccaatccat gtgcaaatga ttgatgaaac tggtcgtacg ctattgcttc
     8101 caattcttgc gatggctggt gcaggacaag tgggtgcagc attggcatta tggattcgct
     8161 gcaaaaaaga taaagaatta acagaaatga tcaaaggagc tttaccagtc gggatcctag
     8221 gaatcggcga accattgatt tacggagtaa cattacctct aggacgccca tttattactg
     8281 cttgtatcgg tggaggtatc ggcggggcag tgatcggaat gatcggaaat gtcggggcta
     8341 tcgctattgg accttcagga gccgctttga tcccattgat ctctgacggt aaatggttag
     8401 gctacatcct tggattattg gcagcttatg ctggtggctt cgttgctaca ttcttcttcg
     8461 gtattcctaa agaccgttta gaaaaagaag aattagcgac tgaaacagcc acagtaaatg
     8521 aagtgcctga aaaagtagta cctcaaactt cagctgaaag tgtgactatc ctctcatcag
     8581 ttgctgacgg aacagttgtt tctttagata aagcaagtga tccagttttt gcacaaaaga
     8641 tgatgggtga aggctacttt gtagaaccag aaaacggaca tatctattct ccagttactg
     8701 gggtagtcag ctctgtcttc ccaacgaaac acgctattgg gatcacaacg ccaaatggtt
     8761 tagaaatctt gcttcatatg ggaatcaaca cagttgactt aggcggtacg ccatttgatg
     8821 tgaaagtaac agaagggcag caagtgacac ctgatacgct tgtagcagaa gctgacttag
     8881 aagcgatcaa agcagcaggc aaagaaacat ccatgatcgt cttggtaacc aatatggatc
     8941 gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcaaa agcaaaagca cctgtgatgg
     9001 atgtcaaagc ttaatatgat agaaacaata gaaacgaaag aagaaactga aatctatttt
     9061 tcagcttctt cttttttatt atttattgtt ttctgctaaa atctgataaa ttcgtgtcac
     9121 atcagatggt gttccccgta attcataatc tgccaagaaa ggaaagccaa atgtttgt
//
LOCUS       DAYYNG010000384         6643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000384 DAYYNG010000000
VERSION     DAYYNG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.149"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(250..486)
                     /locus_tag="ACHBFA_003097"
     CDS             complement(250..486)
                     /locus_tag="ACHBFA_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396472.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(669..1040)
                     /locus_tag="ACHBFA_003098"
     CDS             complement(669..1040)
                     /locus_tag="ACHBFA_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM9396473.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1037..2365)
                     /locus_tag="ACHBFA_003099"
     CDS             complement(1037..2365)
                     /locus_tag="ACHBFA_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9396474.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2326..2655)
                     /gene="mobC"
                     /locus_tag="ACHBFA_003100"
     CDS             complement(2326..2655)
                     /gene="mobC"
                     /locus_tag="ACHBFA_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9396475.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2913..3283)
                     /locus_tag="ACHBFA_003101"
                     /pseudo
     CDS             complement(2913..3283)
                     /locus_tag="ACHBFA_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3428..3613
                     /locus_tag="ACHBFA_003102"
     CDS             3428..3613
                     /locus_tag="ACHBFA_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9396476.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3771..3938
                     /locus_tag="ACHBFA_003103"
     CDS             3771..3938
                     /locus_tag="ACHBFA_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396477.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4556..5215
                     /gene="vanR-B"
                     /locus_tag="ACHBFA_003104"
     CDS             4556..5215
                     /gene="vanR-B"
                     /locus_tag="ACHBFA_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9396478.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5218..6579
                     /gene="vanS-B"
                     /locus_tag="ACHBFA_003105"
     CDS             5218..6579
                     /gene="vanS-B"
                     /locus_tag="ACHBFA_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9396479.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         1652 a         1566 c         1655 g         1770 t
ORIGIN      
        1 tcttttcttt ttgcctattc ctacgcattt agaacccggt attgtcgggg ataggatgaa
       61 gtattcgctt gcccctgcaa acatgcggga gaagccttgc agcgggcggt tttcaatccc
      121 ttaaatgcgt aggttgcagc tataattaga cccctgaaac agatatagcc gcgcagcggg
      181 tgtatctgtt tcaggggtgt gcaggtgata gccgcaaagc ggcgtaagag ggtgcagccc
      241 tcttgccgtc tatctctgtt ccagcgggaa gctcggcaat gcttccagca gtttcaggga
      301 aatgcgctgc cgtatatctt cgtcaatgac tttcttcttt ttgccgtttg cctgtctgat
      361 ctccaccgtg gaaagctcgt tgatgtagtc agcataatgg gccaacactt tttccgtggc
      421 ccacctctca ccggcaacgg cggctttgat cgtttcatag tccagcagct tttcttgtcc
      481 gtccatcctg atacccccgt cagactgatt ttttctatga taccatattc ggaagcatgg
      541 aaccagagaa aacggcgctt ttctgctggt aaattctacg catttagaac agcacttcgg
      601 cggggatagg ataaaacacc cacatacccc gctgaccatg ccggaaaagc cttgaaatat
      661 aagggctttt acagctctaa gtgcgtaggt ttccagtatc ttttccgctg gcgctcggcc
      721 tctttgcggc ggcgcatccg tttggagcag gccgcgcagt atttggcccg gttagacttc
      781 ggcgtgaacg tggccccgca ctcacagcag cgtttcactt cgtcccggtc tttggtgatc
      841 tcggcataca gggccgcatc cagcgggagg acagcggcct taaaccagcg gcacagaagc
      901 gaataggaaa tgctctgtac gcaaatacat tcttcgccgt cgtccagcaa caggcagttg
      961 ccgtcacaat agttgcagca ctcatgcgtc agcttccggg ccttgcgata ttgagggtaa
     1021 ttcattcgtg gaatactcat agctcttgcg tccattcctt tcccggcgtc atacgcaaaa
     1081 tgccgtccac attctgcttg atgatgccgt attcctgcat ctgctttttc gcttcgccgt
     1141 acttctcata cagccgttcc ttttcctcgg ccaatcgctt gtactcggct ttcagggcgt
     1201 acagatcggg gagctttttc acaccctgtc cctttagggc ctttgccgca gcttcataca
     1261 aaatcaacgt gctttcatgc tcacgccgga attttgcctt atccgctgca ttgcggtatt
     1321 ccatagccac gggccagagc tgcttgtagg tagaaatatt ctttatcagc accgccatat
     1381 ccgcaagccg gtgttccata gctttgaggg tagcggcggc ctcgtcgctg gcggcggtga
     1441 tctcggccac tttgctttcc aaatccgggt aggtgtctat cccatgttca gtcaggaaat
     1501 tcaaagtctt tgcggcctgc ttcaaattgt ggagcttcgc ccacctctca tagcctgccg
     1561 actgctgggc cttaatgttg ttttccagat caatccgcaa agaaatcccc ttgcgctctt
     1621 tgggcgctct ggtgataacg cggcctttga tacgctcttt gatggcttcc tcggtgtagg
     1681 cttcccccaa tgtcttgcag cgggtaaagc gttcctgtcc gggagcgcgg aaagaaataa
     1741 actttcccgg cttgatctca tagccctgcg cctgcatgag ccgcaggaaa tcatcaaaat
     1801 cttttgcctg cgggatagcg gcgtcaatgg ctgctttgag cttcgctttc cagctcgtcc
     1861 ctttccgctg ggcgtcccac tcggcatagc tcttgccctt actctggccg ggcttcacca
     1921 cggacaatcc gttttccttg cacagcctgt cgctggtgcg ccggatatag gcatagctct
     1981 gtttgttgga aatgtacttg cgctgattta ccatatccac ggcacagaaa ataatgtgat
     2041 tgtggacgtg gcccttatca atatgggtag tcagcacata gggatatttc ccttgcagca
     2101 cttcctcggc aagctgcttc ccgatctcat gggcctgttc cggcgtggct tcgccggggg
     2161 caaaggattg tatcaggtgg tggcccagat tattgccctt ttggtatgcc tgcgccagca
     2221 gcatttcaaa ttcaatgtcc gctgtttcat aagagcaggc aaaggaggcc accagcagct
     2281 tgccgtcagt tttgtccggg ttctcgatgt agtcaagggc ttttttcagc gttgacttga
     2341 taggatgtat ctttgtaact gccatatctg cgccagcgcc ccctttatat cctcgatgtc
     2401 ctgtgcgtac accggcccca taccgttcag cctctttgca atctgattga cgttgacgcc
     2461 gatcttctgt atctcggcgg tctgcgcccg gatgtcgctg gtgtccatgt tgacgatata
     2521 gccgtcaatg agcatcttcc gggcgtaggc tgaaaagttc ttcgtatgca gcagcgccat
     2581 tttctgttca atcatagccc gttcctccgg cgtcacaggg acgcgcagca cgatttttcg
     2641 ctttctgttt gccatgcttc accgtccttt ccattcaggg gtttggggat ttccccaaca
     2701 agcaaaacgg acgcggcccg gcagggacgg ggcaggccgt ttttccggga gtggctacac
     2761 tccctatgct tgctacgtta ctgtttctcc actaacaaga cgtttccaaa gccccaaact
     2821 acccgcaaaa tagccctctg gaaagaaaaa agtgcaaaaa aatagaagct acgctgtgca
     2881 gctcccgcta aaagtaaaac agaggatgcc agttacacat cctccgtttc attcttcttc
     2941 ggcttttcaa ttagtatggc gctgtcaatc gcgccctcga tgatcggcag gtattcttcg
     3001 gggcagagtt tcagcttgtg gctgaccctt tgccgcagtt cgctttcctc ccgcatgatc
     3061 tccgggttaa agtagcgttc cacaggaagc ccgcaggctt taataagctg tatgacaacc
     3121 ggcagactgg gaatggcccg tcgttttcga tgttggcaag atagcgccag tcaatattta
     3181 ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg
     3241 cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta
     3301 cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta
     3361 tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag
     3421 gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg
     3481 atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat
     3541 ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc
     3601 ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa
     3661 gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga
     3721 taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc
     3781 gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg
     3841 tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct
     3901 ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat
     3961 accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac
     4021 atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt
     4081 ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac
     4141 cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca
     4201 aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg
     4261 ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg
     4321 ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag
     4381 gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc
     4441 atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga
     4501 aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc
     4561 gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct
     4621 ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt
     4681 ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca
     4741 tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct
     4801 gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa
     4861 gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc
     4921 gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact
     4981 ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt
     5041 gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga
     5101 ctttgagggt gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc
     5161 ggaaaatatc atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg
     5221 gaaagaaaag ggattttcat taaggttttt tcctatacga tcattgtcct gctactgctt
     5281 gtcggtgtaa cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa
     5341 ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg
     5401 cttgatattc aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat
     5461 attgaagata aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt
     5521 tttagaccca actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa
     5581 agcaaggcag gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt
     5641 gcgataatgg ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg
     5701 ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg
     5761 ccgctggagc gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc
     5821 aggctgaaag aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag
     5881 gaaacacagc gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct
     5941 acaagcgttc tgttggaggg aatgcttgaa aatatcggtg actacaaaga tcattctaag
     6001 tatctgcgcg aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg
     6061 gagcttgtca gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg
     6121 cgcacggttg ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg
     6181 ttcgtcacag atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag
     6241 gcactatcca atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg
     6301 atatggagtg agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac
     6361 attgatgata ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcga
     6421 agcagaaaaa gtgggcgaag cagaaaaagt gggcgaagcg gtttaggact tgccatcgta
     6481 caaaaaacgc tggatgccat gagcctccag tatgcgctgg aaaacacctc ggatggcgtt
     6541 ttgttctggc tggatttacc gctcacatca acattgtaaa tatttaaaac ttaaatgatt
     6601 ttgggctctt tgtcaaatag gactgataga gtgagataag aac
//
LOCUS       DAYYNG010000385         4702 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000385 DAYYNG010000000
VERSION     DAYYNG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.184"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1000
                     /locus_tag="ACHBFA_003106"
     CDS             194..1000
                     /locus_tag="ACHBFA_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9396480.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1018..1725
                     /locus_tag="ACHBFA_003107"
     CDS             1018..1725
                     /locus_tag="ACHBFA_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9396481.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1722..2693
                     /locus_tag="ACHBFA_003108"
     CDS             1722..2693
                     /locus_tag="ACHBFA_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9396482.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2686..3714
                     /gene="vanB"
                     /locus_tag="ACHBFA_003109"
     CDS             2686..3714
                     /gene="vanB"
                     /locus_tag="ACHBFA_003109"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9396483.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3720..4328
                     /locus_tag="ACHBFA_003110"
     CDS             3720..4328
                     /locus_tag="ACHBFA_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9396484.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4394..4567)
                     /locus_tag="ACHBFA_003111"
     CDS             complement(4394..4567)
                     /locus_tag="ACHBFA_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396485.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1329 a          986 c         1245 g         1142 t
ORIGIN      
        1 gatagaatga ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata
       61 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
      121 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
      181 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
      241 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
      301 ctgcacgctg tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc
      361 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
      421 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
      481 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
      541 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
      601 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
      661 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
      721 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
      781 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
      841 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
      901 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
      961 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     1021 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     1081 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     1141 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     1201 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     1261 ttaaacggct tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg
     1321 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     1381 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     1441 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     1501 caggtgttcg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     1561 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     1621 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     1681 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     1741 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     1801 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     1861 ctggcaatca atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg
     1921 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     1981 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     2041 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     2101 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     2161 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     2221 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     2281 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     2341 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     2401 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     2461 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     2521 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     2581 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     2641 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     2701 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata
     2761 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     2821 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     2881 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     2941 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     3001 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     3061 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     3121 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     3181 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
     3241 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
     3301 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
     3361 attgtcggcg aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa
     3421 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
     3481 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
     3541 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
     3601 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
     3661 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
     3721 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
     3781 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
     3841 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
     3901 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
     3961 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
     4021 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
     4081 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
     4141 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
     4201 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
     4261 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
     4321 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
     4381 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
     4441 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
     4501 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
     4561 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
     4621 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
     4681 gttgaactat ttaatggact gt
//
LOCUS       DAYYNG010000386         3732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000386 DAYYNG010000000
VERSION     DAYYNG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.211"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACHBFA_003112"
     CDS             <1..420
                     /locus_tag="ACHBFA_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9396486.1"
                     /translation="NTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYESGKMPKEVRDQ
                     QLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGFEADQLAEAF
                     GLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            774..2252
                     /gene="msr(C)"
                     /locus_tag="ACHBFA_003113"
     CDS             774..2252
                     /gene="msr(C)"
                     /locus_tag="ACHBFA_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9396487.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2299..2745
                     /locus_tag="ACHBFA_003114"
     CDS             2299..2745
                     /locus_tag="ACHBFA_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9396488.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2944..3459)
                     /locus_tag="ACHBFA_003115"
     CDS             complement(2944..3459)
                     /locus_tag="ACHBFA_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9396489.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3576..>3732
                     /locus_tag="ACHBFA_003116"
     CDS             3576..>3732
                     /locus_tag="ACHBFA_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9396490.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQL"
BASE COUNT         1208 a          619 c          782 g         1123 t
ORIGIN      
        1 aatacgagac aaaatgatac ttcttctgca atggtcatga tttttggcga tatgcaatgt
       61 tacgaatatg gcgaagaaat ctataaccaa gcttacgaat cagggaagat gccaaaagaa
      121 gtcagagatc aacaattagc tgcgatcatt ccttattaca agagtttttc aagagaagaa
      181 atgaacgatg tagtcaaagt cgattctagc ttagcggcta tgcaattcat gttagttgcg
      241 cgtgatcatg gctatgaaac aaatccaatt ggtggattcg aagcagatca gttggcagag
      301 gcatttggtt tggataaaga ccgttatgtt ccagttatta ttttatcaat aggaaaagcg
      361 gtagaagaag gttacgagtc tgttcgttta gcaccagaaa agatcactac gttcgaataa
      421 aaggttttca gttactattc agtagctatt gctttttttg aatcgtgtgt ggtaacctaa
      481 taaaaaagga ggtcggaaaa atgactgcat cgatgaaatt acgtttcgaa cttttgaata
      541 acaactaaat aaattcaaaa gacatgtata aacaacaatt tcatggatgg agtatgtcca
      601 ttttttcgaa cgacagtaga ataggtgaaa gctatgacag atttggtgat ttagtcatag
      661 ttatttcgtt cagagtagga aaagatgtga tgcagttttt ttgcaaacag atttttctgc
      721 tcttttttag tatcgataat tgttgtttta catgaaatag gagtgtaaaa catatggaaa
      781 atttagcagt aaatataaca aatctacaag tcagctttgg caaccagcta gaactatcta
      841 ttgattctct tcgtgtctat cagcaagatc gtatagggat cataggagaa aatggtgtcg
      901 gtaaatcgac tttgctcaaa ctaatagccg gtgaactttt tcccgatcat gggaaaatcc
      961 aaacagagat caccttcaac tacctgcctc aattaaccta tctcgctgaa gcaaaggacc
     1021 taaatttgga attagccagt catttccagt taagactgga agaaacttca gagcggaaat
     1081 ggagcggagg ggaagaacga aagatcgagt tgatacgtct tctttcttct tatgaacaag
     1141 ggatgcttct agacgagcca acaacccatc tagatagaaa aagtattgat cgactgattg
     1201 aagagcttcg ttattattat ggtacgctgg tttttgttag tcatgatcgc tattttctag
     1261 atgagctagc atcgaaaatc tgggaagtaa aagacggaga aatccgagag ttttcgggga
     1321 attatagtgc ctatctcact caaaaggaat tggagaaaaa gactcagcta cgagaagcag
     1381 agtcgatcat gaaagagaaa aaacgattgg aaaaatcgat ccaagaaaag aaaaaacaag
     1441 cggaaaagtt ggaaaaagtg tccagtaaaa agaaaaagca acaaatcaga ccggatcggt
     1501 tgtcttcctc taaacaaaaa gacagtgtac aaaaagccat ccaaaagaat gcgaaaacat
     1561 tagagagaag actccaaaaa ataggagaaa caaccaagcc gcagcagatg aaacaaatcc
     1621 gttttccagt accaaaatct cttgaactcc acagccgcta tcccatcatg ggacaaaatg
     1681 tccaattgga aagaagtggg agaacattac tggtaaatgg tgattttcag ttttctttag
     1741 gtaaaaaaat cgcgattgtc ggtgaaaatg gttcaggtaa gacaacctta ttagaacata
     1801 tccgcaaaca aggagaagga atccttctct ctccgaaagt aagctttcaa gtatatcagc
     1861 aaaagggtta tcaaatgaca tctgaagaat ccatcattcg ttttgtcatg agacaaacag
     1921 agttttcaga atcgcttgtc cgtagtttgc tgaatcactt agggtttgct caggaaactt
     1981 tgacgaaacc gttatgtaca ttaagtgggg gagaagcgac ccgtctgacg attgctttgc
     2041 tttttactaa gccaagtaat gtgttgctgt tagatgaacc gactaatttt attgatatgg
     2101 caacgatcga agctttagag aagctaatgc aaatatatcc gggaacgatt ttgtttactt
     2161 ctcatgattc ttactttgtc gagcgtacgg ctgatgaagt ttatgaaata aaagggcaga
     2221 aaataaaaaa agtacttacg agaaattttt aaatgtgcta cgatgatttt cgtgacaaat
     2281 ttaaaggagt ggtacagatt gcctacaaat aaaaaagaag gaataatttt tacaacaatg
     2341 atgtgttttt tgatggtctt ggggatgagc gcgtataatt tatggctgca tcaagatctt
     2401 catttatcaa gtttgttgat tggtctagtg cctggattcg tggtggcttt cattttggat
     2461 gtcttcatca tcggtgtgct ggcgaaaaaa attgccttta aactaccagt aaatccagaa
     2521 agcaaggtcc aaatgatctt aactatttca tgcttgatgg ttttaggaat ggtgacatgt
     2581 atgtctttat tcggtgtgat catggaacaa ggaatacctg atcaattatt ctctgcttat
     2641 atgaatgctt ggaaaatgaa tgtgattgcc gctttaccgt tgcagttgat catcgttggc
     2701 ccgctttcaa gaagtgtgct aaaagcaatt caaagtaatg aatgatggaa cactgttatt
     2761 tgaaaaaagg ccttggcatg ctttttgtca tagtctcttg aatgcagttc tagaaaaata
     2821 gtgatccgaa cgataggttt tggcctgtcg ttcggatcgc tttttctttt ccaactgtat
     2881 tgctctttta ttgaagataa tttgctcttc aatagatatt tctagaaagg ttctgtttat
     2941 cccttactca aaaaactgct gcatttgttc tcttacttgc tgacaccatt ggagttcctg
     3001 ctgacaaagc ataattctgc gctggatcac aacataacgt ccaaattctt gtttcttgtc
     3061 tagcgagagg tcattcaatt ttttttgatt tttttgcatg attttgttca gctgttgttc
     3121 tctagtgaat agcagtccag tagctgttgt tttatcgagc gtcgaaaaag catagatttt
     3181 tgctagaaat tcatcttttt gcgtaggagc gggagtttcc tctttgatcc attcctctac
     3241 aagagcacgt ccttcatcag tcaagtggta gatctttttt tgagagtgct cgtctttgtc
     3301 caaaacggtc acataaccct gctggtctaa cttggcaagc gttgtataga tctgactgtg
     3361 atgggcttcc caaaaaattg tgatcagctg cttcaattca tacccagaac gtggtttgga
     3421 taaaagcacg cataataata tataggctaa agtattcata aaaatcctac ctctcgtctt
     3481 attttcttta ttatatcaca gtaaagtttc cacgttttac atgtgcatat ttacatgtaa
     3541 aataaatcat gtatgatgct aatagagaga aagggatgga aatattggag aatcaaatag
     3601 atatacaaga acaaacaaat gaagaaacaa aggggaaaag ttcagcgcta gttgtagcag
     3661 ctacctgtat gatcgctttt atgggagtgg gtctagtaga cccaatctta aaaacgattg
     3721 ctgcacagtt aa
//
LOCUS       DAYYNG010000387         3604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.217, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000387 DAYYNG010000000
VERSION     DAYYNG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.217"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHBFA_003117"
     CDS             <1..1076
                     /locus_tag="ACHBFA_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9396491.1"
                     /translation="VSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSV
                     LGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTH
                     EIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFW
                     ASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKIC
                     FKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDA
                     PKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGE
                     PVCQTRRLNSRIDKVRYMFNKIT"
     gene            1174..1362
                     /locus_tag="ACHBFA_003118"
     CDS             1174..1362
                     /locus_tag="ACHBFA_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9396492.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1420..1773)
                     /locus_tag="ACHBFA_003119"
     CDS             complement(1420..1773)
                     /locus_tag="ACHBFA_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396493.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2304..2726
                     /locus_tag="ACHBFA_003120"
     CDS             2304..2726
                     /locus_tag="ACHBFA_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9396494.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2723..2953
                     /locus_tag="ACHBFA_003121"
     CDS             2723..2953
                     /locus_tag="ACHBFA_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9396495.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3451..>3604
                     /locus_tag="ACHBFA_003122"
     CDS             3451..>3604
                     /locus_tag="ACHBFA_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM9396496.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAV"
BASE COUNT         1196 a          591 c          676 g         1141 t
ORIGIN      
        1 gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat
       61 tatgtaagat tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga
      121 agttaaatag tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc
      181 cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc
      241 ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta
      301 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
      361 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
      421 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
      481 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
      541 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
      601 gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta
      661 agtacggttt atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta
      721 ttgtactgga gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt
      781 ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt
      841 gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc
      901 ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg
      961 tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc
     1021 gtcgtctaaa tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg
     1081 ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata
     1141 gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt
     1201 cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc
     1261 cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata
     1321 ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct
     1381 catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc
     1441 ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc
     1501 catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa
     1561 aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg
     1621 gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc
     1681 cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc
     1741 tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata cattttactg
     1801 ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata
     1861 gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt
     1921 tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc
     1981 gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt
     2041 aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt
     2101 ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg
     2161 tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc
     2221 ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc
     2281 acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc
     2341 agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc
     2401 tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact
     2461 ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa
     2521 gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac
     2581 ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac
     2641 taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag
     2701 aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat
     2761 tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac attatcgagg
     2821 gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt ctcatatggt
     2881 tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga ttttatcgtt
     2941 cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca cgcttcccga
     3001 tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt tgataggcta
     3061 acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc ggtaagaaaa
     3121 gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat gttagtaatt
     3181 cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa tgatactccc
     3241 gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct acatgagaac
     3301 catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta agatattctt
     3361 ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat attggagttt
     3421 tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa ggatttagtt
     3481 gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa agaattgatg
     3541 attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc tcgtgaagct
     3601 gttg
//
LOCUS       DAYYNG010000388         3293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.225, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000388 DAYYNG010000000
VERSION     DAYYNG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.225"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..788
                     /gene="dfrG"
                     /locus_tag="ACHBFA_003123"
     CDS             291..788
                     /gene="dfrG"
                     /locus_tag="ACHBFA_003123"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9396497.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(860..2812)
                     /locus_tag="ACHBFA_003124"
     CDS             complement(860..2812)
                     /locus_tag="ACHBFA_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9396498.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2819..3055)
                     /locus_tag="ACHBFA_003125"
     CDS             complement(2819..3055)
                     /locus_tag="ACHBFA_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9396499.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1021 a          568 c          543 g         1161 t
ORIGIN      
        1 ggcgtatata tgcaagtaag tagttcagtt ttggagtaca aaatgacaaa atgtacttca
       61 aaatggacta cttttttcat tttaacttac ataccaaatt acatacaaaa tgatttataa
      121 aatgcaattg aaaatgataa ttgaatgaaa atttgtgatt ttgtggtatt atgataatat
      181 aacttataga aaaaattgct tattcaacta acgggcagga taatgagaaa aggttttttg
      241 atagaaataa gaaattagat ggtaaattag ttaaaaaggc tgtgttataa atgaaagttt
      301 ctttgattgc tgcgatggat aagaatagag tgattggcaa agagaatgac attccttgga
      361 ggattcccaa ggactgggaa tatgttaaaa atactacaaa gggacatccg ataatattag
      421 gtaggaagaa ccttgaatca atcggaagag ccttacctga cagaagaaat attattctga
      481 cgagagataa ggggtttacc tttaatggtt gtgaaattgt tcattcaata gaagatgttt
      541 ttgagttatg taaaaacgaa gaagaaattt ttattttcgg aggagaacag atttataatt
      601 tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat acatcatgaa ttcgaaggag
      661 atactttttt tccagaagtg aattatgagg aatggaatga ggtatttgcc caaaaaggga
      721 taaagaatga taaaaatccg tataactact attttcatgt atatgaaaga aaaaacttat
      781 tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga taaagcacaa gagcttggga
      841 ttactccaag ctctttttgt catttgaaat aaattatatc tttccaatca aattgtttat
      901 ctgtaatccc taatctttga gcaggtgtca aaaccttttt atccgctgat ttaaaagggc
      961 gacaaaagtt gtagtaagtt cttagaattg taatcgcaaa ttgagcatac ttcggattga
     1021 agttagcgta aatatagctc ttcttatcac cacgagctgt tgttaatggt ctttcaagag
     1081 atgatatata acgtcttatc tgttgaataa agctattggt cgaatggtca ttaacattta
     1141 atagcatctt tgcgatttcg tttggttcca gagcagataa atccgttcta caatcaactg
     1201 aataaaatcc tttatcctta gtggctaatg gatgtttgat agggttatcg gcatatttgc
     1261 gatatctttc tccatcttta ctaatagcct cttcgtggaa ttgatgactt gttagtaatt
     1321 cagttagata atgagatgcc aaagtccgta gaaactttgt tttgaatcct ctgatatctc
     1381 cccaatcgag cagttctgct tttgcttgtc cgaattcttt taagcattgt tttctgcttt
     1441 tcgtcttgtt aacttggcaa ataaaatgat gtgcatcgct caaccttaat tctttcgtga
     1501 atactctata aaaggcgttt ctaatagaga agtcatcatc actaatcatt cgccattcgt
     1561 cggcattaac catttgttta attaaccaat agtgagcagt tgtagtataa ggagcatcaa
     1621 catttaaacc atcgacaaat ctactcctat tagtaatttt ccctaattcg tgaagataag
     1681 cattcctatt ttcgctatcg ttagcggatg gttcttgtgg ataataagac caatctaacc
     1741 tatcattttt acgactaaaa gtattgagat ggtcttcttt gaattttctt gtatcttcat
     1801 ttaattcatc cattgaaata ttccaatcat aggcaacatc agaacggaac acatatcttg
     1861 ataagacctc agcagataca actatatacg tttgcatatt aaggtcttca aaccccgtat
     1921 atttctttga accttgtcct ttcttacgga cgttatttag gtagtagtgc atcttgtccg
     1981 tattaagcca catctcgtta aatttctttg tttgaagggg ttgtgtctca tatcgttcta
     2041 aaaactccaa acaacgccta tacagccact ccagcttatg atagtaggtt ccaactccaa
     2101 ttcctaaaat atcacaagta cgactgactg aaacccttcc aacaaccatc ttagcgaaca
     2161 ttggaagaat ggtgttcttt tgttggtgat aagtagtggt ttcttctctt tttggtagaa
     2221 cattagtgaa cttcttacac gccttacatt gatagcgttg agatttgccc ctacttttcc
     2281 cccttttgta aaattgcttc ggctcattga atggggttga ttcttcttcc gagcagcctt
     2341 ctttatgaaa ttgataatct ggctcaacat cttggatgct attgatttct ataaggcgtt
     2401 taatttcttc aacaacagac caattagata aaggggttac tgtacagttt tgcgatacac
     2461 ctctgccaat aggattgtca ttgcagtaca tacctttatg acctttatct ttggaactac
     2521 cagtcatttt atatcgactt ggtttccctt ttacatcttt gaatttgtgc tgttctttcc
     2581 caaaattaca acaaaacgga ttgatgcagt aattatattg aatgggatat tcagaaccgt
     2641 tccatttaaa agacgttggc aagaacaaca ttttgttata ccttgttgca aatcgttttg
     2701 gagttaataa atcatattgt ttagccctat cttttaattc ttcgtttgaa atcggaattt
     2761 taacatctac aatttcatct tccttcgtgg ctaatctttt aagcttggtc attgtctatc
     2821 aacccactct atctttgata cccatttttt caacaatccg cttattactt cgcttgttgg
     2881 caatccattc aataaacttc ttatcgtcta aaatgttcaa taagaattca tccactgctt
     2941 cgctttcagt acgctctgca tattcagcgt attgcttaac tatcaccctt acttgctctg
     3001 atatttccca atccacagac ttagcgttct tattttttgg ctcaagaaat tgcataggaa
     3061 aatgcacccc tttttgctta gtaaattgta accattttaa cacaccaatt tgcattccta
     3121 caataattat ttcacgttcg catttttata tacattttaa cttacatttt gtggtgtaaa
     3181 ttaacaacaa acgttgattt aacaataaaa aacgagtatc aaattcagtt gcaatttgat
     3241 actcatttag gtttacattt tcactttttg tactccaaaa ctgaactact taa
//
LOCUS       DAYYNG010000389         1359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.293, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000389 DAYYNG010000000
VERSION     DAYYNG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.293"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..340
                     /locus_tag="ACHBFA_003126"
                     /pseudo
     CDS             261..340
                     /locus_tag="ACHBFA_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            389..472
                     /locus_tag="ACHBFA_003127"
     CDS             389..472
                     /locus_tag="ACHBFA_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM9396500.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            594..1334
                     /gene="erm(B)"
                     /locus_tag="ACHBFA_003128"
                     /pseudo
     CDS             594..1334
                     /gene="erm(B)"
                     /locus_tag="ACHBFA_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
BASE COUNT          489 a          206 c          225 g          439 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc ttttgtacat ttaacttgac
       61 ggtgacatct ctctattgtg agttattagt ggtacagttt tcaaccgttt taattataaa
      121 aaagtggtgc atttttaaat tggcacaaac aggtaacggt tattgcaggt gtatttctta
      181 tctatgggtt taacatggat tttatcatta aaatcatgag tattgtccga gagtgattgg
      241 tcttgcgtat ggttaaccct aaagttatgg aaataagact tagaagcaaa cttaagagtg
      301 tgttgatagt gcattaaaaa ttttgtataa taggaattga agttaaatta gatgctaaaa
      361 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgtt atgtagataa
      421 aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta
      481 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa
      541 caaatcgttt aacttctgta tttgtttata gatgtaatca cttcaggagt gattatatga
      601 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
      661 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
      721 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
      781 atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc
      841 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggagtattc
      901 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat gcgtctgaca
      961 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag
     1021 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct
     1081 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg
     1141 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat
     1201 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca
     1261 atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta
     1321 acgggaggaa ataattctat gagtcgcttt tgtaaattt
//
LOCUS       DAYYNG010000390         1332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.296, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000390 DAYYNG010000000
VERSION     DAYYNG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.296"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            617..1165
                     /gene="aac(6')"
                     /locus_tag="ACHBFA_003129"
     CDS             617..1165
                     /gene="aac(6')"
                     /locus_tag="ACHBFA_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9396501.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          464 a          198 c          263 g          407 t
ORIGIN      
        1 catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac
       61 tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc
      121 tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt
      181 ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt
      241 gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc ctttgctttt
      301 ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg ttttatttgt
      361 caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg aacaaacaga
      421 gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa gtgtttctgt
      481 ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac tgattggtag
      541 ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga ataagcaaga
      601 acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag tattgaaaga
      661 tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca gctcggcaga
      721 agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag accaagatga
      781 gttagtagga tttattggtg caatccctca atacggtatc acaggttggg aattgcatcc
      841 attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag tcaattactt
      901 agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg atgatttaga
      961 ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata aagtggcttc
     1021 catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt ataaaatcgt
     1081 aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg caaaaacgat
     1141 tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa tcagcttcaa
     1201 gaaataagaa ggaatttacg aaaattactt cttgaatttt tgtgaaattc caacttattt
     1261 ccgaagaagc tacttttgga acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc
     1321 gagtagtaag aa
//
LOCUS       DAYYNG010000391         1229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.301, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000391 DAYYNG010000000
VERSION     DAYYNG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.301"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1103
                     /locus_tag="ACHBFA_003130"
                     /note="aph(2'')-Ia"
     CDS             <1..1103
                     /locus_tag="ACHBFA_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFM9396502.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          529 a           95 c          201 g          404 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttacca
//
LOCUS       DAYYNG010000392         1092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.313, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000392 DAYYNG010000000
VERSION     DAYYNG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.313"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..139
                     /locus_tag="ACHBFA_003131"
     CDS             <1..139
                     /locus_tag="ACHBFA_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9396503.1"
                     /translation="IGCSEMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVYDYEKGD
                     K"
     gene            140..>1092
                     /gene="aac(6')-Ie"
                     /locus_tag="ACHBFA_003132"
     CDS             140..>1092
                     /gene="aac(6')-Ie"
                     /locus_tag="ACHBFA_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFM9396504.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDI"
BASE COUNT          458 a           86 c          187 g          361 t
ORIGIN      
        1 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
       61 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      121 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      181 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      241 tgatggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      301 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      361 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      421 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      481 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      541 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      601 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      661 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
      721 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
      781 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
      841 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
      901 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
      961 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1021 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1081 tacagatatt ag
//
LOCUS       DAYYNG010000393          714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6573
            SAMEA115708459-rid22599133.guided.340, whole genome shotgun
            sequence.
ACCESSION   DAYYNG010000393 DAYYNG010000000
VERSION     DAYYNG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708459
            Sequence Read Archive: ERR13198182
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290284.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290284.1
            Genome Coverage       :: 113x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:26:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,133
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,973
            CDSs (with protein)               :: 2,973
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 43 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 27 of 96
            Pseudo Genes (multiple problems)  :: 17 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708459-rid22599133.guided.340"
                     /isolate="VRE6573"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..403
                     /gene="fosX"
                     /locus_tag="ACHBFA_003133"
                     /pseudo
     CDS             6..403
                     /gene="fosX"
                     /locus_tag="ACHBFA_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339390.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FosX/FosE/FosI family fosfomycin resistance
                     hydrolase"
BASE COUNT          220 a          134 c          118 g          242 t
ORIGIN      
        1 ataaaatgtg ccaaagaatt aaagctatga attattcagg tttaaatact taattgttaa
       61 ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt cattatatcc
      121 agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt cgtcgaataa
      181 actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata tagattcgtt
      241 tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg
      301 cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt tacataccgg
      361 tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt aatgactagt
      421 ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt cgaattgatc
      481 tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga ttcatcacaa
      541 atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat gctcgtgtgg
      601 gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac atttttgtct
      661 cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct tggg
//
